LOCUS       DATCUN010000001         4803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_18798, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000001 DATCUN010000000
VERSION     DATCUN010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18798"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VIO83_00005"
     CDS             <1..268
                     /locus_tag="VIO83_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329707.1"
                     /translation="RPTEGYRMAGLDELRQLPHPQGQHSLYDRRGLVPGTQRLERWLL
                     WPSGVVSPGAMRQWGRHASAFVGQAHFDDPDLLGRYFPLPPTQK"
     gene            complement(313..1374)
                     /gene="lptG"
                     /locus_tag="VIO83_00010"
     CDS             complement(313..1374)
                     /gene="lptG"
                     /locus_tag="VIO83_00010"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEY8329708.1"
                     /translation="MVRLDRYIGSSVFFSILGVLGVIVGLALLFAFIDELGDLDAGYG
                     VAEAARYIAFTIPARIYELLPMAALIGCLVGLGSLASSSELTVMRAAGVSLGRIVGAV
                     MKPLLVLLVVGLLIGEYVVPWTQSEAQSGRSLAQGGGEAQTTKRGLWHRQGEEFIHIN
                     SVQPNGVLLGVTRYRFDDERRLLSASFARRAQYQGDYWQLENVATTLLHEDRSEVLQA
                     PSERWDVELTPQLLGTVVVEPEALSMTGLWRYIHYLGEQGLNNSRYWLAFWTKALQPL
                     VTATLVLLAISFIFGPLRSVTLGQRLFTGVLVGFIFRIAQDMLGPSSQVFGFPPLLAV
                     LVPAGICGLVGVWLLRRAG"
     gene            complement(1361..2530)
                     /gene="lptF"
                     /locus_tag="VIO83_00015"
     CDS             complement(1361..2530)
                     /gene="lptF"
                     /locus_tag="VIO83_00015"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011535496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEY8329709.1"
                     /translation="MIIFRYLSREVLVTFSAVSAVLLVIIMSGRFIKYLAQAAQGLLD
                     PGVLFMIMGFRLPGFLQLILPLGLFLGILLAYGRLYLESEMTVLAAAGMSRKRLLVYT
                     LAPAALVAAVVAWLSLSLAPQGVTQVERILYSQDALTEFDTLVPGRFQELKSGSRVTY
                     TEALSEDGRELGGVFISELRLSRSGNKERGISVLVAEKGRQEIRADGSRYLILENGYR
                     YDGKPGQADYRAIQYDRYGVLLPKPEVAGELNEREGIPTAELISGKSLEQIPRAGLRE
                     LQKRRHQAELQWRLSLPLLVFVVTVLAVALARANPRQGRFLKLLPAILLYMAYLALLI
                     AMRGRVEEGKLPSALGLWWVHALFLAIGLLLMCRESLRLKWMARRVAKEAARGQA"
     gene            2681..4168
                     /locus_tag="VIO83_00020"
     CDS             2681..4168
                     /locus_tag="VIO83_00020"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY8329710.1"
                     /translation="MQFLVKSARPEALKTATLVLAVGEGRKLGAAARAVDAASEGALS
                     VLLKRGDLAGKPGQTLLLHNLPGIKAERVLLVGCGKERELSDRNYRKVVSAALAVLKT
                     LGGSDAALTLGELMVQGRDLYARTRLLVEVLADGRYLFDRFKSEKADTPALKKITLLV
                     EETEIAETERGAAHGQAIASGMALTRDLGNLPPNLCHPSFLADEARALAKQFKNLKVE
                     VLDEKKLKELGMGAFLAVAQGSEQPPRLIVLEYKGGKKDEQPFALVGKGITFDTGGIS
                     LKPGAGMDEMKYDMCGAASVLGTFRALVELQLPINVVGLLACAENMPSGGATRPGDIV
                     TTMSGQTVEILNTDAEGRLVLCDTLTYAERFKPQAVIDIATLTGACIVALGSHTSGLL
                     GNHDALIKQILKAGEHAGDRAWQLPLFDEYQEQLDSPFADIANIGGPKAGTITAACFL
                     SRFAKKLHWAHLDIAGTAWISGGKDKGATGRPVPLLTQYLLERCK"
     gene            4209..4628
                     /locus_tag="VIO83_00025"
     CDS             4209..4628
                     /locus_tag="VIO83_00025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003375978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEY8329711.1"
                     /translation="MKRIDFYILPDSDAAARLTYACRLAAKAWREGHRIYLHCQDAAQ
                     CAALDERLWSFSAASFVPHQLAEDGNDAPLLLAWGPDAGGEQSLLINLASTVPAFFER
                     FARIAEVVNQDPLTRDALRTSYRFYRERGYAPQHLHL"
BASE COUNT          858 a         1763 c         1536 g          646 t
ORIGIN      
        1 caggccgacc gagggctacc gcatggccgg gctggacgag ctgcgccagc tgccccaccc
       61 ccaggggcag cacagcctgt acgaccgccg cgggctggtc ccgggcaccc agcgtctgga
      121 gcgctggctg ctgtggccct ccggggtggt ctcgccgggc gccatgcgcc agtgggggcg
      181 gcatgccagc gccttcgtcg gccaggcgca tttcgatgac ccggacctgc tggggcgcta
      241 ttttcccctg ccgccgacgc agaaatgaac aaggccggcg cagggccggc cttgttgctc
      301 gccctggcgt cgtcagccgg cccggcgcag cagccagacc ccgaccaggc cgcagatgcc
      361 cgccggcacc agcaccgcca gcagcggcgg gaagccgaac acctggctgg aagggccgag
      421 catgtcctgg gcgatgcgga agatgaagcc caccagcaca ccggtgaaca gccgctgacc
      481 gagggtcacc gagcgcaacg ggccgaagat gaaggaaatc gccagcagca ccagggtcgc
      541 ggtgaccagc ggctgcaggg ccttggtcca gaacgccagc cagtagcggc tgttgttcag
      601 gccctgctcg cccagatagt ggatgtagcg ccacagcccg gtcatcgaca gcgcctccgg
      661 ctcgaccacc acggtgccga gcagttgcgg cgtgagctcg acatcccagc gctcgctggg
      721 cgcctggagc acctcgctgc gatcctcgtg cagcagcgtg gtggcgacgt tctccagctg
      781 ccagtagtcg ccctgatact gcgcgcggcg agcaaaactg gcgctgagca ggcgccgctc
      841 gtcgtcgaag cgatagcggg tcaccccgag cagcacgccg ttgggctgca ccgagttgat
      901 atggatgaac tcctcgccct ggcgatgcca caggccgcgc ttggtggtct gcgcctcacc
      961 gccgccctgg gccagcgaac gcccgctctg cgcctcgctt tgcgtccagg gcacgacgta
     1021 ctcgccgatc agcagaccga ccaccagcag caccagcagc ggcttcatca ccgcgccgac
     1081 gatgcgcccc agcgataccc cggcagcccg catgaccgtc agctcgctgc tgctggccag
     1141 cgagccgagg ccgaccaggc agccgatcag cgccgccatc ggcagcagct cgtagatccg
     1201 cgccgggatg gtgaacgcga tgtagcgggc ggcttcggcc acaccataac cggcgtccag
     1261 atcacccagc tcatcgatga aggcgaacag cagcgccagg ccgacgatga cccccagcac
     1321 cccgaggatg ctgaagaaca cactgctacc gatataacga tcaagcctga ccacgagcgg
     1381 cctccttcgc gacgcggcgc gccatccact tcagccgcag cgactcgcga cacatcagca
     1441 acaagccgat ggcgaggaac agcgcgtgca cccaccacag gccgagcgcc gagggcagct
     1501 tgccctcctc cacccggccg cgcatggcga tcagcagggc cagataggcc atgtacagca
     1561 ggatcgccgg cagcagcttg aggaagcggc cctggcgcgg gttggcgcgg gccagcgcca
     1621 cggccagcac ggtgaccacg aacaccagca gcggcagcga cagacgccac tgcaactcgg
     1681 cctgatggcg ccgcttttgg agctctcgca gacccgcgcg gggtatctgt tccagactct
     1741 taccgctgat caactcggca gtcgggatgc cctcgcgctc gttcagctcg ccggccactt
     1801 ccggcttggg cagcagcacg ccgtagcggt cgtactggat cgcccggtag tcggcctggc
     1861 cgggcttgcc gtcgtagcga tagccgttct ccaggatcag gtagcggctg ccgtcggcac
     1921 ggatctcctg gcggcccttt tccgccacca gcacgctgat cccccgctcc ttgttgccgc
     1981 tgcgcgacaa gcgcagttcg gagatgaaca ccccgcccag ttcgcggccg tcctcggaca
     2041 gcgcttcggt ataggtcact cgcgagccgc tcttcagctc ctggaaacgc cccggcacca
     2101 gggtatcgaa ctcggtcagg gcatcctggc tgtagagaat gcgctcgacc tgggtcaccc
     2161 cctgcggcgc cagcgacagg ctcagccagg ccaccacggc cgccaccagc gccgccggcg
     2221 ccagggtgta gaccagcagg cgcttgcggc tcatgccggc ggcggccagc acggtcattt
     2281 ccgactcgag atagaggcgc ccgtaggcca gcaggatgcc gaggaacagg cccagcggca
     2341 ggatcagctg caggaagccg ggcaggcgaa agcccatgat catgaacagc acgcccggat
     2401 cgagcagccc ttgggccgcc tgcgccagat acttgatgaa gcgcccgctc atgatgatca
     2461 ccagcagcac ggcgctcacc gcactgaagg taaccagtac ttcgcgggac aggtaacgaa
     2521 agatgatcaa accaggacta ctccagggtt gtcaggctcg ggccgcaacg ctcaaactag
     2581 ccgtcaacca cgccggctgg cgcagcatcg ctgcccgcag aatgaaaata ggccggcatt
     2641 atcctgcaaa gccgaccgtt tgtcttgggg acccccgtcc atgcaatttc tcgtcaagag
     2701 cgcccgtccg gaagccctga aaaccgccac cctggtgctc gccgtcggcg agggccgcaa
     2761 gctcggagcc gccgcccgtg ccgtcgacgc ggccagcgag ggcgccctga gcgtcctgct
     2821 caagcgcggc gacctggccg gcaagccggg ccagaccctg ctcctgcaca acctgccagg
     2881 gatcaaggcc gaacgcgtgc tgctggtcgg ctgcggcaag gagcgcgaac tctccgaccg
     2941 caactaccgc aaggtagttt ccgccgccct cgccgtgctc aagaccctgg gcggcagcga
     3001 cgccgcactc accctcggcg agctgatggt gcagggccgc gacctgtacg cgcgcacccg
     3061 cctgctggtg gaagtgctgg ccgacggccg ctacctgttc gatcgcttca agagcgagaa
     3121 ggccgacacc ccggcgctga agaagatcac cctgctggtc gaggaaaccg agatcgccga
     3181 aaccgagcgc ggcgccgccc acggccaggc catcgccagc ggcatggcgc tgacccgcga
     3241 cctcggcaac ctgccaccga acctgtgcca cccgagcttc ctcgccgacg aggccagggc
     3301 gctggccaag cagttcaaga acctcaaggt cgaggtgctc gacgagaaga agctcaagga
     3361 actgggcatg ggcgccttcc tcgccgtggc ccagggcagc gagcagccgc cgcggctgat
     3421 cgtgctcgag tacaagggcg gcaagaagga cgagcagccc tttgccctgg tcggcaaggg
     3481 catcaccttc gacaccggcg gcatcagcct caagccgggt gccggcatgg acgagatgaa
     3541 gtacgacatg tgcggcgccg ccagcgtgct cggcaccttc cgcgccctgg tcgagctgca
     3601 gctgccgatc aacgtggtcg gcctgctcgc ctgtgccgag aacatgccca gcggcggcgc
     3661 cacccgcccc ggcgacatcg tcaccaccat gagcgggcag accgtggaga tcctcaacac
     3721 cgacgccgaa ggccgcctgg tgctgtgcga caccctgacc tacgccgaac gcttcaagcc
     3781 gcaggcggtg atcgacatcg ccaccctcac cggcgcctgc atcgtcgccc tcggcagcca
     3841 tacctccggc ctgctgggca accacgacgc gctgatcaag cagatcctca aggccggcga
     3901 gcacgccggc gaccgcgcct ggcagctgcc gctgttcgac gagtaccagg agcagctgga
     3961 cagcccgttc gccgacatcg ccaacatcgg cggccccaag gccggcacca tcaccgccgc
     4021 ctgcttcctg tcgcgcttcg ccaagaagct gcactgggcg cacctggaca tcgccggcac
     4081 cgcctggatc agcggcggca aggacaaggg cgccaccggt cgcccggtgc cgctgctgac
     4141 ccagtacctg ctcgaacgct gcaagtgagc cgcttccggc cggcgttcgc gccggccgga
     4201 gccgaccgat gaagcgcatc gacttctaca tcctccccga tagcgacgcc gccgcgcggc
     4261 tgacctatgc ctgccgcctg gcggccaagg cctggcgcga ggggcaccgc atctacctgc
     4321 actgccagga tgcggcgcag tgcgcggcac tggacgagcg gctgtggagc ttcagcgccg
     4381 ccagcttcgt gccgcaccag ctggccgagg acggcaacga cgccccgctg ctgctggcct
     4441 gggggccgga tgccggcggc gagcagagcc tgctgatcaa cctggccagc acggtgccgg
     4501 cgttcttcga gcgcttcgcg cgcatcgccg aagtggtcaa ccaggacccg ctgacccgcg
     4561 acgccctgcg cacgagttac cgcttctacc gcgaacgcgg ctatgctccc caacacctgc
     4621 atctgtaacg cgcgagccgc cgatggacac tcccccgctt ccaccgcaac ccgccaacct
     4681 gcccgactgg gccaatgacg aactgccgct actgaccgag acggtcggcg agccgggcat
     4741 tccgctgctg tccgaggtcg tgccggctac gccgcgcccc gccgcgagcg agccgaccag
     4801 tcc
//
LOCUS       DATCUN010000002         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_75122, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000002 DATCUN010000000
VERSION     DATCUN010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75122"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /gene="gatB"
                     /locus_tag="VIO83_00030"
     CDS             <1..1024
                     /gene="gatB"
                     /locus_tag="VIO83_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY8329712.1"
                     /translation="DLNRAGTPLLEIVSEPDMRSAKEAVAYVKTIHALVRYLGICDGN
                     MAEGSLRCDCNVSVRPKGQAEFGTRAEIKNVNSFRFIEKAINHEVQRQIELIEDGGKV
                     VQETRLYDPNKDETRSMRSKEEANDYRYFPCPDLLPVVLEDDFIEAVRASLPELPNEK
                     RARFESEHGLSAYDASVLSASRELADYFEEVQKVCGDAKLAANWVMGELSSLLNAENL
                     SIEQSPVSAAQLGGMILRIKDNTISGKLAKMVFEALAAGEGDSADAIIEAKGLKQVTD
                     SGAIEAMLDEVLAANAAQVEQYRASDEAKRGKMFGFFVGQAMKASKGKANPGQVNELL
                     KKKLEG"
     gene            1185..1559
                     /locus_tag="VIO83_00035"
     CDS             1185..1559
                     /locus_tag="VIO83_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008015285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY8329713.1"
                     /translation="MRRALPILLLVLLAGCASRDQASAPSDYRAEGQASYYSSRHHGR
                     RTASGERFDMHALTAAHRSLPFGSKVKVTNVKNQRSVVVRINDRGPYARGRIIDVSRK
                     AAERLGMLGSGVTQVRLERLPD"
     gene            1662..>2542
                     /locus_tag="VIO83_00040"
     CDS             1662..>2542
                     /locus_tag="VIO83_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEY8329714.1"
                     /translation="MTLMTFAYLIAGLILLVAGAEVLVRGAARLAAQFGIPPLIIGLT
                     VVAFGTSAPEMAVSVQSAFAGQGDIAVGNVVGSNIFNVLFILGLSALITPMLVSRQLI
                     RFDVPIMIGASLLAWFLAMDQRYGRLDGVLLFGGVLAYTGFLIVGSLRARNNGASEEF
                     EDEYGIQEPSGSFASLRHLAFIAAGLVLLVYGSGLLVDGAVSLAKALGLSELVIGLTI
                     IAAGTSLPEVATSVIAALKGERDIAVGNVVGSNIFNLLCVLGLASLVSPTPLSVSANA
                     IAFDYPVMIAVAMACLPV"
BASE COUNT          445 a          889 c          825 g          383 t
ORIGIN      
        1 cgacctgaac cgcgccggca cgccgttgct ggagatcgtg tccgagccgg acatgcgcag
       61 cgccaaggaa gccgtggcct acgtcaagac gatccacgcg ctggtgcgct acctgggcat
      121 ctgcgacggc aacatggccg agggctcgct gcgctgcgac tgcaacgtct cggtgcgccc
      181 caagggccag gccgagttcg gcacccgcgc cgagatcaag aacgtcaact cgttccgctt
      241 catcgagaag gcgatcaacc acgaggtgca gcggcagatc gagctgatcg aggatggcgg
      301 caaggtggtg caggaaaccc gcctgtacga cccgaacaag gacgagacgc gctcgatgcg
      361 cagcaaggaa gaggccaacg actaccgcta cttcccctgc cccgacctgc tgccggtggt
      421 gctggaggac gacttcatcg aggccgtgcg cgccagcctg ccggaactgc ccaacgagaa
      481 gcgtgcgcgc ttcgagagcg agcatggcct gtcggcctac gacgccagcg tgctgtcagc
      541 cagccgcgaa ctggccgact acttcgagga agtgcagaag gtctgcggcg acgccaagct
      601 ggcggccaac tgggtgatgg gcgagctgtc cagcctgctc aacgccgaaa acctttcgat
      661 cgaacagtcg ccggtctccg ccgcgcagct cggcgggatg atcctgcgca tcaaggacaa
      721 caccatctcc ggcaagctcg ccaagatggt gttcgaggcg ctggccgccg gcgaaggcga
      781 ctcggccgat gccatcatcg aagccaaggg cctcaagcag gtcaccgaca gcggcgccat
      841 cgaagccatg ctcgacgaag tgctggccgc caacgccgcg caggtcgagc agtaccgcgc
      901 cagcgacgaa gccaagcgcg gcaagatgtt cggcttcttc gtcggccagg cgatgaaggc
      961 ctcgaagggc aaggccaacc ccggccaggt caacgaactg ctgaagaaga agctcgaagg
     1021 ctgagccaac gggcaacccc tgcgttgcct tcgcaagcgc ccggccaggc atggccgggc
     1081 cggagctgtc gacaggtatc gaagtcacgg cacggatgtc cgctccagcc cgaaatcctc
     1141 ccgccgccgg actcgaccgg cggcgggcct tccggagtcc cgacatgcgc cgagcactgc
     1201 ccatcctcct cctcgtcctg ctggccggct gcgccagccg cgaccaggcc tccgccccca
     1261 gcgactaccg cgccgaaggc caggcctcgt actacagcag ccgccaccat ggccgccgta
     1321 ccgccagcgg cgaacgcttc gacatgcacg cgctgactgc cgcccatcgc agcctgccgt
     1381 tcggcagcaa ggtcaaagtc accaatgtga aaaaccagcg cagcgtggtg gtgcgcatca
     1441 acgaccgcgg cccctacgcc cgcgggcgga tcatcgacgt ctcgcgcaag gcggccgaac
     1501 gcctgggcat gctgggcagc ggcgtgacgc aggtgcgcct ggagcgcctg cccgactgaa
     1561 ccagcaatgc agcacgcggt aacaatcggc tctggcccgg ggctggcagc gcggcggcaa
     1621 aacgctaagc tggcgcgcaa cttgtccaga gcatccaagc catgaccctg atgaccttcg
     1681 cctatctgat cgccggcctg atcctgctgg tcgccggcgc cgaagtactg gtacgcggcg
     1741 ccgcgcgcct ggccgcccag ttcggcattc ccccgctgat catcggcctg accgtggtcg
     1801 ccttcggcac cagcgcgccg gaaatggcgg tcagcgtgca atcggccttc gccggccagg
     1861 gcgacatcgc cgtcggcaac gtggtcggca gcaacatctt caacgtgctg ttcatcctcg
     1921 gcctgtcggc gctgatcacg ccgatgctgg tgtcgcgcca gctgatccgc ttcgacgtgc
     1981 cgatcatgat cggcgccagc ctgctggcct ggttcctggc catggaccag cgctacggcc
     2041 gtctcgacgg cgtgctgctg ttcggcggtg tgctggccta taccggcttt ctgatcgtcg
     2101 gcagcctgcg cgcccgcaac aacggcgcca gcgaggaatt cgaggacgag tacggcattc
     2161 aggagccgag cggaagcttc gccagcctgc gccacctggc gttcatcgcc gccggcctgg
     2221 ttctgctggt gtatggctcc ggcctgctgg tggacggcgc ggtcagcctg gccaaggcgc
     2281 tgggcctgtc ggaactggtg atcggcctga ccatcatcgc cgccggaacc tcgctgccgg
     2341 aggtggccac ctcggtgatc gccgccctca agggcgagcg cgacatcgcc gtgggcaacg
     2401 tggtcggcag caacatcttc aacctgctct gcgtgctcgg tctggcctcg ctggtgtcgc
     2461 caacgccgct gagcgtgtcg gccaacgcca tcgccttcga ctacccggtg atgatcgccg
     2521 tggccatggc ctgcctgccg gt
//
LOCUS       DATCUN010000003         6679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_18834, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000003 DATCUN010000000
VERSION     DATCUN010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18834"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="VIO83_00045"
     CDS             complement(<1..1333)
                     /locus_tag="VIO83_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEY8329715.1"
                     /translation="MVRVRKLVLAIAAASALSSGMAHALGLGEISLKSSLNQPLDAEI
                     ELLEVRDLGSNEVLPSLAAVEEFSKVGVDRPFFLTDLKFTPVLKANGKSVIRVTSNKP
                     VREPYLNFLVEVLWPNGRLLREYTVLLDPPLYSPQTVVAASPRLPQAAPVAPRVSVAP
                     QAPVSRPQSVAPAAAPAAVAPAPSRIDGDQYRTDRDDTLWEVASRVRPSSAVSVHQTM
                     LAIQDLNPDAFLDGNINRLKRSQVLRLPTEQQIRSRSQADALAQVQAQNGAWREGRAV
                     QPVAARQLDATRRSEAGNAPAQAPAEDNLRLVAAASGKAASGRDNGAAGDGKALADKL
                     AVSQESLVTSQRENQELKERLTDLQSQLDKLQRLIELKNEQMAKLQGGLAADAQAMPA
                     AVAAAAATAQPAAAAPAADAAATAPAVAPAPVGVPAAADAVAPATPAPAPAA"
     gene            complement(1471..2463)
                     /locus_tag="VIO83_00050"
     CDS             complement(1471..2463)
                     /locus_tag="VIO83_00050"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018928095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY8329716.1"
                     /translation="MNETFDIAVIGATGLVGEALLMVLDEREFPVGNLFPLSAEAAVG
                     QIAAFRERNLRVRALDSFDFATVALVFVVEPLAPAQLVRIEAAGCALVDLSGSLPEWQ
                     APCVVAGVNAPAAVGRWLRSPLPAAVALASVLGNLRTVLQLRRVTATACLAVSSHGRE
                     GVEELARQTAQLLNARPVEPQLFDRQIAFNLLARASAEMPDGHARDERRLAGELLEVL
                     GEPQVPVAITCVQAPVFFGDSLAVSLLCAEPVDLPVVLHCLDDAPDLELVEQGDYPTA
                     VGDALGQDLVYVGRVRAGLHDPCELNLWIASDNVRKGSALNAVQLGELLLKPHL"
     gene            complement(2658..3770)
                     /gene="asd"
                     /locus_tag="VIO83_00055"
     CDS             complement(2658..3770)
                     /gene="asd"
                     /locus_tag="VIO83_00055"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY8329717.1"
                     /translation="MKRVGLVGWRGMVGSVLMQRMLEERDFDLIEPVFFTTSNVGGQG
                     PAVGKDIAPLKDAYSIDELKSLDVILTCQGGDYTSEVFPKLREAGWQGYWIDAASSLR
                     MNDDAVIVLDPVNRKVIDQALDNGGKNFIGGNCTVSLMLMALGGLYEAGLVEWMSAMT
                     YQAASGAGAQNMRELIKQMGAIHHAVADDLANPASAILEIDRKVAETQRSAEFPVDHF
                     GVPLAGSLIPWIDKELPNGQSREEWKAQAETNKILGRNKSPIPVDGLCVRIGAMRCHS
                     QALTIKLNKDVPLADIEGLISQHNPWVKLVPNQREISMRELSPTAVTGTLSVPVGRLR
                     KLNMGSQYLGAFTVGDQLLWGAAEPLRRMLRILLER"
     gene            complement(3831..4913)
                     /gene="leuB"
                     /locus_tag="VIO83_00060"
     CDS             complement(3831..4913)
                     /gene="leuB"
                     /locus_tag="VIO83_00060"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY8329718.1"
                     /translation="MSKQILVFPGDGIGPEIVAEAVKVLELANEKYQLGFELSYDELG
                     GAAYEKYGTPLADETLERARAADAILLGAVGGPQWDNLDRPLRPEMGLLKIRSQLGLF
                     GNLRPALLYPQLAGASSLKPEIVAGLDILIVRELTGGIYFGSPRESRVLENGERMAFD
                     TLPYSESEIRRIAKVGFDMARLRGKKLCSVDKANVLESSRLWRAVVEEVAKDYPDVEL
                     SHMYVDNAAMQLVRAPKQFDVMVTDNMFGDILSDEASMLTGSIGMLPSASLDANNKGM
                     YEPCHGSAPDIAGQGIANPLATILSVSMMLRYSFGQLEAADAIERAVGNVLDQGLRTG
                     DIWSEGCQKVGTRAMGDAVVAALRNL"
     gene            complement(4977..5624)
                     /gene="leuD"
                     /locus_tag="VIO83_00065"
     CDS             complement(4977..5624)
                     /gene="leuD"
                     /locus_tag="VIO83_00065"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY8329719.1"
                     /translation="MKAFTQHTGLVCPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFD
                     EWRYLDVGQPNQDCANRPVNKEFVLNFPRYQGASVLLARENFGCGSSREHAPWALEEY
                     GFRTIIAPSYADIFFNNSFKNGLLPIILPEAEVDELFQQCEAAEGYQLTVDLAAQTVT
                     RPDGKVLSFDIDPFRKHCLLNGLDDIGLTLQDADAIKAFEAKHQQSSPWLFGAIK"
     gene            complement(5636..>6679)
                     /gene="leuC"
                     /locus_tag="VIO83_00070"
     CDS             complement(5636..>6679)
                     /gene="leuC"
                     /locus_tag="VIO83_00070"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY8329720.1"
                     /translation="MTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLVAKKMKN
                     MQVRVEGQLPAGVTAKDIVLAIIGKIGTAGGNGHALEFAGSAIRELSMEGRMTICNMA
                     IEAGARVGLVAVDDKTIAYVEGRTFAPKGADWNKAVEQWQGLVSDADAHFDTVVELQA
                     EDIKPQVSWGTSPEMVLPVDANVPDPAQEADPVKRDSIVRALKYMGLTANQPITTIQL
                     DRVFIGSCTNSRIEDLRAAAVVAKGRKVAATVKQALVVPGSGLVKAQAEAEGLDKIFI
                     EAGFEWREPGCSMCLAMNPDRLESGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAA
                     VTGHFVDVRELLN"
BASE COUNT         1104 a         2221 c         2220 g         1134 t
ORIGIN      
        1 cagctgccgg agccggcgcc ggagtagctg gtgcgacagc atcggctgca gcaggtacgc
       61 cgacaggtgc aggagcgacc gccggggcag ttgccgcagc gtccgcagcc ggagctgcgg
      121 cagccggctg ggcagtagcg gcagcggcgg ctacagcggc aggcatggcc tgggcatcgg
      181 cggccaaacc accctgcagc ttggccattt gttcgttctt cagctcgatc agacgctgca
      241 gcttgtccag ctggctttgc aggtcggtga ggcgctcctt gagctcctga ttctcgcgct
      301 gcgaggtgac caggctttcc tgactgacgg ccagcttgtc ggccagcgcc ttgccgtcac
      361 cggcagcacc gttgtcgcga ccactggcgg ccttgccgct ggcggcggcc accaggcgca
      421 gattgtcttc ggccggcgcc tgcgccgggg cattgcccgc ctcgctgcgg cgagtggcgt
      481 cgagctggcg ggcagccacc ggctgcacgg cacgaccttc gcgccaggcg ccgttctgcg
      541 cctgaacctg agccagggca tcggcctggc tgcggctgcg gatctgctgc tcggtcggca
      601 ggcgcagcac ctggctgcgc ttcaggcgat tgatattgcc atcgaggaag gcgtccggat
      661 tgagatcctg gatcgccagc atggtctggt gcaccgaaac ggcgctgctc gggcgcacgc
      721 gactggcgac ttcccacagc gtgtcgtcac gatcggtacg gtactggtcg ccgtcgatgc
      781 ggctcggcgc cggagcgacg gcggcaggcg cggcagccgg ggcgacggac tgcggacggc
      841 tgaccggagc ctgcggcgct acgctgacac gcggcgccac cggcgcagcc tgcggcagac
      901 gcgggctggc ggcgaccacg gtctgcggcg agtagagtgg cggatcgagc agcacggtgt
      961 actcgcgcag cagacggcca ttgggccaca gcacctcgac caggaagttc agatagggct
     1021 cgcgcaccgg cttgttcgag gtgacgcgaa tcacgctctt gccattggcc ttgagtaccg
     1081 gagtgaactt caggtcggtg agaaagaaag gccgatcgac gcccaccttg ctgaactctt
     1141 cgacggcagc cagcgagggc agcacctcgt tgctacccag atcgcgcacc tcgagcagct
     1201 cgatttccgc atccagcggc tggttcaggg aggacttcag cgatatttca cccaacccca
     1261 gcgcatgcgc catgccggag gacagcgccg aagcggcggc gattgctagc accagtttgc
     1321 gaacccgaac catagcctgt attccctgtt ttttctgctg tgctcgccca gcgagtcagt
     1381 ggcatggctg gcatctgcgc ccgcgatgtg gcggcccccc agatgcacag tcaagatgaa
     1441 aaagcttaca aattttgcgg caagtatctt ttacaggtgg ggtttaagca ataactcgcc
     1501 cagctgcacg gcattgagtg ccgaaccttt tcgcacgtta tctgatgcaa tccacaaatt
     1561 gagttcgcac ggatcatgca gcccagcgcg tacccgtccg acatacacca gatcctggcc
     1621 gagcgcgtcg ccgacggcgg tgggatagtc gccctgctcg accagttcga ggtccggcgc
     1681 atcgtcgaga caatgcagca ccaccggcag gtcgaccggc tctgcacaca acaggctgac
     1741 cgccagactg tcgccgaaga acaccggcgc ctgcacgcag gtgatggcca ccggcacttg
     1801 cggctcgccc agcacctcga gcaactcgcc ggccagacgg cgctcgtcgc gggcatgacc
     1861 gtccggcatc tctgccgaag cgcgggccag caggttgaag gcgatctgcc gatcgaacag
     1921 ttgcggctcc accgggcggg cattgagcag ctgcgcggtc tgccgcgcca gctcctcgac
     1981 cccctcgcgc ccgtggctgg acaccgccag acaggccgtg gccgtcaccc ggcgcagctg
     2041 cagcaccgtg cgcaggttgc cgagtacgct ggccagcgcc accgcggccg gcagcgggct
     2101 gcgcaaccag cgcccgaccg ctgccggcgc attgaccccg gcgaccacgc agggcgcctg
     2161 ccactccggc agcgagccgc tcagatcgac cagcgcacaa ccggcggcct cgatccgtac
     2221 cagctgtgcc ggcgccagcg gctcgaccac aaacaccagc gccacagtgg cgaaatcgaa
     2281 ggagtccagc gcgcgcacgc gcaggttgcg ctcgcgaaac gcggcgatct gcccgaccgc
     2341 cgcctcggcg gacaggggga acagattgcc gaccggaaac tcgcgctcgt caaggaccat
     2401 cagcagcgcc tcgccgacca ggccggtggc accgatgacg gcgatatcga aagtctcgtt
     2461 catgaagcgg cctcctgcga aatagggccg cactttagcg agcgcgccgg caattgggta
     2521 gccagcccat gcgcaggcgg accagcggaa cgcgccgcaa ctgacctacg acaagcctta
     2581 cccacaaatg acaagacccg ccgcagcggg tcttgtcagg gttcagcagg cttagcggcg
     2641 cattacacgc cctccattca acgctccagc aggatgcgca gcatgcggcg cagcggctcg
     2701 gccgcgcccc acagcagctg gtcgccgacg gtgaaggcgc cgaggtactg cgagcccatg
     2761 ttgagcttgc gcagacggcc gaccgggacg ctgagggtac cggtcaccgc ggtcgggctc
     2821 agctcgcgca tgctgatctc gcgctggttc ggcaccagct tgacccacgg gttgtgctgg
     2881 ctgatcaggc cttcgatgtc ggccagcggc acatccttgt tcagcttgat ggtcagcgcc
     2941 tggctgtggc agcgcatggc gcctatgcgc acgcacaggc cgtcgaccgg gatcgggctc
     3001 ttgttgcgac cgaggatctt gttggtctcg gcctgagcct tccattcctc gcggctctgg
     3061 ccgttgggca gttccttgtc gatccacggg atcaggctgc cggccagcgg cacgccgaag
     3121 tggtccaccg ggaactcggc gctgcgctgg gtctcggcga ccttgcggtc gatctcgagg
     3181 atggcgctgg ccgggttggc caggtcgtcg gccaccgcat gatggatcgc gcccatctgc
     3241 ttgatcagct cgcgcatgtt ctgcgcgccg gcgccggaag cggcctggta ggtcatggcg
     3301 ctcatccact cgaccagacc ggcttcgtac aggccgccga gtgccatcaa catcaggctg
     3361 acggtgcagt tgccgccgat gaagttcttg ccgccgttgt ccagcgcctg gtcgatgacc
     3421 ttgcggttga ccggatcgag gacgatcacc gcgtcatcgt tcatgcgcag gctggaggcc
     3481 gcgtcgatcc agtagccctg ccagccggct tcgcgcagct tggggaagac ttcgctggtg
     3541 tagtcgccac cctggcaggt caggatcacg tccagggact tcagctcgtc gatgctgtag
     3601 gcatccttca gcggggcaat gtccttgccg acggccggac cctggccacc cacgttggag
     3661 gtggtgaaga acaccggctc gatgaggtcg aaatcgcgct cttccagcat ccgctgcatg
     3721 agcacggaac ccaccatacc gcgccaaccg acaagaccaa cacgcttcat cgcaactaca
     3781 cctttctcga aaaagtgggg ccgcccgcgg cggggcggct cccggacagc ttacagattc
     3841 cgcagcgcgg cgactactgc gtcgcccatc gcgcgggtac cgaccttctg gcagccttcc
     3901 gaccagatgt cgccggtgcg caggccctgg tccagcacat tgccgaccgc gcgctcgatg
     3961 gcgtcggcag cctcaagctg gccgaagctg tagcgcagca tcatcgacac cgagagaatg
     4021 gtcgccagcg ggttggcgat gccctggccg gcgatgtccg gcgccgagcc gtggcacggc
     4081 tcgtacatgc ccttgttgtt ggcgtccagc gaggccgacg gcagcatgcc gatcgaacca
     4141 gtgagcatcg aggcttcgtc ggagaggatg tcgccgaaca tgttatcggt gaccatcacg
     4201 tcgaactgct tgggcgcgcg gaccagctgc atggcggcgt tgtcgacgta catgtgcgac
     4261 agctcgacgt ccgggtagtc cttggccact tcctcgacca cggcgcgcca caggcggctg
     4321 gactccagca cgttggcctt gtccaccgag cagagcttct tgccgcgcag gcgggccatg
     4381 tcgaagccga ccttggcgat gcggcggatc tcgctctcgc tgtacggcag ggtatcgaac
     4441 gccatgcgct cgccgttttc cagcacgcgg ctttcacgcg ggctgccgaa gtagatgcca
     4501 ccggtcagct cgcggacgat gagaatgtcc aggccggcga cgatctccgg cttgaggctg
     4561 gaggcgccgg ccagttgcgg gtagagcagc gccggacgca ggttgccgaa caggcccagc
     4621 tgcgagcgga ttttcagcag gcccatttcc ggacgcagcg gacggtcgag gttgtcccac
     4681 tgcgggccgc cgacggcgcc gagcaggatg gcgtcggcgg cgcgggcgcg ctccagggtc
     4741 tcgtcggcca gcggggtgcc gtacttctcg taggcggcgc cgcccagctc gtcgtagctc
     4801 agctcgaagc ccagctggta cttctcgttg gccagctcca gtaccttgac cgcttcggcg
     4861 acgatttccg gaccaatacc atcacccggg aacaccagaa tctgcttgct cattgcatgt
     4921 ccttgtaaat gtcgaaccgc gggagcgagc cctgcccgct cccgccgaaa agggggttac
     4981 ttgatcgcgc cgaacagcca ggggctgctc tgctggtgct tggcttcgaa cgccttgatc
     5041 gcgtcggcat cctgcaaggt caggccgatg tcgtccagac cgttgagcag gcagtgcttg
     5101 cggaacggat cgatgtcgaa ggacaacacc ttgccgtccg ggcgggtcac ggtctgcgcc
     5161 gccaggtcga cggtcagctg gtagccctcg gccgcctcgc actgctggaa cagctcgtcc
     5221 acttccgctt ccggcaggat gatcggcagc aggccgttct tgaagctgtt gttgaagaag
     5281 atgtcggcgt agctcggcgc gatgatggtg cggaagccgt actcttccag cgcccacggc
     5341 gcgtgttcgc gggaggagcc gcagccgaag ttctcgcggg ccagcagcac gctggcgccc
     5401 tggtagcgcg ggaagttgag cacgaactct ttattcaccg gacggttcgc gcagtcctgg
     5461 ttcggctggc cgacgtccag gtagcgccac tcgtcgaaca ggttcggacc gaagccggtg
     5521 cgcttgatcg acttgaggaa ctgcttggga atgatctggt cggtgtcgac gttggcgcga
     5581 tccagcgggc agaccaggcc ggtgtgttgg gtaaaggctt tcattcagac gatcctcagt
     5641 tcagcagctc acgcacatcg acgaagtgac cggtcaccgc ggccgcggca gccatggccg
     5701 ggctgaccag gtgagtacgc ccaccggcac cctggcggcc ctcgaagttg cggttggagg
     5761 tggaggcgca gtgctcgcca ctctccaggc ggtccgggtt catcgccagg cacatggagc
     5821 agcccggctc gcgccactcg aaaccggcct cgatgaagat cttgtccagg ccctcggcct
     5881 cggcttgcgc cttgaccaga ccggagcccg gcaccaccag cgcctgcttg acggtggcag
     5941 cgaccttgcg gcccttggcc accacggcag cggcacgcag gtcctcgatg cgcgagttgg
     6001 tgcaggagcc gatgaacacg cggtccagct ggatcgtggt gatcggctgg ttcgcggtca
     6061 gccccatgta cttgagggcg cgaacgatgg agtcgcgctt gaccgggtcg gcttcctgcg
     6121 ccggatccgg cacgttggca tcgaccggca gcaccatctc cggcgaggtg ccccagctga
     6181 cctgcggctt gatgtcctcg gcctgcagct cgaccacggt gtcgaagtgg gcgtcggcat
     6241 cggagaccag cccctgccac tgctccacag ccttgttcca gtcggcgccc ttgggcgcga
     6301 aggtgcgacc ttcgacgtag gcgatggtct tgtcgtccac ggcgaccaga ccgacgcggg
     6361 cgccggcctc gatggccatg ttgcagatgg tcatgcgacc ttccatcgac agctcgcgga
     6421 tcgcgctgcc ggcgaattcc agggcatggc cattgccgcc ggcagtgccg atcttgccaa
     6481 tgatcgccag cacgatgtcc ttggcggtga cgccggcggg cagttggccc tccacgcgca
     6541 cctgcatgtt cttcatcttc ttggccacca ggcactgggt cgccagcacg tgctcgacct
     6601 cggaggtgcc gatgccgtgg gccagcgcgc cgaaggcgcc gtgggtggag gtgtgcgagt
     6661 cgccgcagac cacggtcat
//
LOCUS       DATCUN010000004         6317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_150210, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000004 DATCUN010000000
VERSION     DATCUN010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150210"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..739
                     /locus_tag="VIO83_00075"
                     /pseudo
     CDS             153..739
                     /locus_tag="VIO83_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726349.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
     gene            complement(820..3471)
                     /locus_tag="VIO83_00080"
     CDS             complement(820..3471)
                     /locus_tag="VIO83_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY8329721.1"
                     /translation="MPNNSFGESSCTAAASGLVQVRGARENNLKEVDVSIPRNALVVF
                     SGVSGSGKSSLAFGTIYAEAQRRYFESVAPYARRLIDQAGVPDVDAIDGLPPAVALQQ
                     QRGSSNARSSVGSVTTLSSLVRMMYSRAGAYPANQPMLYAEDFSPNTPQGACPTCHGL
                     GHVYEVTEAIMVPNPSLSIRERAIASWPPAWQGQNLRDILVSMGYDVDRPWKDLPKKD
                     RDWILFTEETPTVPVYAGFTPAETRAALKRKMEPSYMGTFTGARRYVLHTFANTQSAL
                     MRKRVSRFMEGKPCPTCHGKRLKPEALSVTFAGVDIGAFMQMPLDQLAALLEPIAQGD
                     FRAHAAGAATDKEATRRDRAERAASGRAVHAVSPDVRRTSALSEEKRLAAQRLAGGVM
                     ARLRQLRGLGLGYLTLDRATPTLSAGELQRLRLATQLSSLLFGVVYVLDEPSAGLHPS
                     DSQALYDALDRLRDAGNSVFVVEHDLDLMRRAQWLVDVGPDAGERGGRVLYSGEPDGL
                     RKIAESRTARYLFEEIPAPGSRAREATGWLELQGIHRHNLHGVDARIPLGVLTAVTGI
                     SGSGKSSLVAQALPELVLLHLGHEPEDDAAESATSEGPAVIEATGGHLAGDVDAIQRL
                     VQVDQKPIGRTPRSNLATYTGLFDHVRKLFAATPDARRRRYDAGRFSFNVAKGRCETC
                     EGEGFVSVELLFMPSVYAPCPTCHGARYNEATLKVQWNGRNIAEVLQMTVDEASEFFA
                     GEDAVARPLQLLRDIGLGYLRLGQPATELSGGEAQRIKLATELQRSQRGRSLYVLDEP
                     TTGLHASDADRLLVQLQRLVDAGNTVVMIEHDMRAVAQADWVIDVGPGAGAAGGSIVV
                     AGSPQQVARTSGSRTAPFLAQELARAE"
     gene            complement(3497..5362)
                     /gene="nhaA"
                     /locus_tag="VIO83_00085"
     CDS             complement(3497..5362)
                     /gene="nhaA"
                     /locus_tag="VIO83_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEY8329722.1"
                     /translation="MPDASLGESSASIRRRRVARYLHTESGAAVLLVIVTVVALAWAN
                     SPLSNAYFELWHLDVGFDFGPLGLHMDLHHWVNDGLMVVFFFLIGLEVRQEFAHGSLR
                     DRSRARLALIAGVAGVVLPALVYVLIVGLAGSEGLHGWGAVVGTDTAFMLGTLAIVGP
                     RLSGQLRVFLLTLTVVDDFLAVSIIGIVYSEEIRVVPLLIALASLVGLWLLGRNRQWR
                     ATPYVLIVIVLWLATVYSGIHASLAGMAAGLLIPAYATQRHKVVAARQLFRDFWQSPS
                     AASARAVDCGLSRGISVNERLHEFLRLPTALLIVPMFALANAGVDLRGGLLAEAFGSP
                     VTWGVVAGLVLGKLLGIGLITLAAVRLGLGRLPAGVGMGSVFGGAALSGIGFTVSLLI
                     IGLAFGTTSDLGRQATVGVLVSMMLATLLGRLIFKVAARRWGEETADLPMVLEPPVDP
                     EVDHIRGPEDAQLTLVEYVDFECAYCAHATGSWEDLRAHFGDDLRYVVRQLPHHPHGP
                     IAARASEAASNQGMFWPWLDFVFTRQHALEREDLIGYAVELGLDVDRFIADLDSPAVI
                     ERVERDLASAVASGAHATPTFFVEGRRLRGSYDARTVTAALEASRRGTRTQEVPS"
     gene            complement(5418..>6317)
                     /locus_tag="VIO83_00090"
     CDS             complement(5418..>6317)
                     /locus_tag="VIO83_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009876623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination factor protein RarA"
                     /protein_id="HEY8329723.1"
                     /translation="TTEHPGLAVNSALLSRAQVYTLEPLSSDELNQLYERALPHLQGV
                     TLDTDALDLLKGFADGDGRRFLNLLEQVTNAASGAGKTVVDGEFAKLSTSPSLRRFDK
                     GGDEFYWQLSAFHKSLRGSDPDAALYWLARILDGSGDVSQVTRRMVVMASEDIGNADP
                     QALQLALNAALAYDRLGSPEGEIPLAQAVTYLVLAPKSNATYAAWDQAKAFVKRSGSQ
                     PVPLHLRNAPTKLMKQMGYHEGYRYAHDEPNGYAAGQTYFPEGVARPGWYQPTDRGVE
                     RRLGERLTWLRSLDDATPPSPDA"
BASE COUNT         1141 a         2127 c         2037 g         1012 t
ORIGIN      
        1 atctgccagt cgcagcgcat aagcgatttg ctcctctgaa aacctcgacc ttttcattgc
       61 ctctccttgt gccctttggg gcctgccgga aagcaaccgt aactctagtt ttgaaaagtc
      121 cggaattcgg gggagacgtc accaccatcg tttcaccgcc cgcagcccag cccgtgcccg
      181 acgtcatgga ggatttgctc gcgatggtgg gcttgggtgt cggtcggcgt tggccaggat
      241 gccgaggagt tcctccacac ccccgcgcca gcaacagccg cgacgtccat tccatcgcct
      301 gcacctgcac ccgcgcctac gcctgggcca tcggcaacga agccatccgg ggaacagttc
      361 atggtttggc tgaagcaggg aatcgcatca cgacggctca tcatcaacga cgcgaaggca
      421 ctcgtgcata cggtgaatga cacggcctac ctacctggtc agcccgggtg tgttccaacg
      481 ctatgcgcag gagcatcccg aagtggccgc actcgccaag caggagaatc aacaggattg
      541 gcaatgggtg cagaagcgct tcgagaagct gctgcagcta catcgaaagc agcccaatgg
      601 cctgaacatt tggacctgtg aagtcacggg cccgaggaag tcccgccgac tgcatggcta
      661 cctcctcgaa gatgggtcct tggcactcgc cgaaataccg cccaacaacc gctatcttgc
      721 tctgactcag gagggatgac gcgattcggg cattgatcgc caccgtcgtg tggcgacgat
      781 caatgccccg cgaaaattgg caacatttgg cgaactgagc tactcggccc gcgccaactc
      841 ctgtgcaagg aacggagcgg ttctgctgcc agacgttcgg gccacctgct gaggggagcc
      901 cgccaccacg atgctgccgc cggcagcgcc tgcaccaggc cccacgtcga tcacccaatc
      961 ggcctgggcc accgcgcgca tatcgtgttc gatcatcact acggtattgc cggcatcgac
     1021 caggcgctgc aactgcacca gcagccggtc ggcgtccgac gcatgcagcc cggtagtcgg
     1081 ctcgtcgagc acgtacaggc ttcggccgcg ctggctgcgc tgaagctcgg tcgccagctt
     1141 gatacgctgc gcctcgccac cggaaagctc ggtggccggt tgccccaggc gcaggtagcc
     1201 cagtccgata tcgcgcagca gttgcagtgg ccttgccaca gcgtcttcac ccgcgaagaa
     1261 ttcgctggct tcgtccacgg tcatctgcag cacctcggcg atgttgcgcc cgttccattg
     1321 caccttcagc gtggcctcgt tgtagcgtgc gccatggcac gtcgggcacg gcgcgtacac
     1381 gctgggcatg aacagcagtt ccacgctgac gaagccctcg ccctcgcagg tctcgcagcg
     1441 ccccttggcg acgttgaacg agaaacggcc ggcgtcatag cggcggcggc gagcgtcggg
     1501 cgtggcggcg aacagcttgc gcacgtggtc gaacaggccg gtgtaggtag ccaggttcga
     1561 gcgcggcgtg cgcccgatcg gcttctggtc cacctgcacc aggcgctgta tggcgtccac
     1621 atcgcctgcc agatgaccgc cagtcgcttc gatcaccgcc ggcccttcgc tggtggcgct
     1681 ttcggccgca tcgtcttccg gctcgtggcc caggtgcagc agcaccaact ccggcagggc
     1741 ctgcgcgacg aggctggact tgccggagcc ggagataccg gtgacggccg tcagcacgcc
     1801 cagcggaatg cgtgcatcca cgccatgcag gttgtggcgg tggatgccct gcagttccag
     1861 ccagccggtc gcttcgcgtg cccggcttcc cggcgcgggg atctcctcga acagatagcg
     1921 ggcggtgcgc gattcggcaa tcttgcgcag gccatccggc tcgccgctgt agagcacgcg
     1981 gccgccacgc tccccagcat ccggcccgac atccaccagc cattgcgcgc ggcgcatcag
     2041 gtccaagtcg tgctccacca cgaacaccga gttgccggcg tcgcgcagcc ggtcgagcgc
     2101 gtcgtacagg gcctggctat cggaggggtg caggcccgcg gagggctcgt cgagcacgta
     2161 cacgacaccg aacagcaggg aactcaattg cgtggccagc cgcaggcgct gcaactcgcc
     2221 ggccgaaagc gtcggcgtgg cccggtccag cgtcaggtag cccaggccca gcccacgcag
     2281 ttggcgcagg cgtgccatca cgccaccggc caggcgctgc gcggcgaggc gtttttcttc
     2341 cgacagtgcg gaggtgcggc gcacgtcggg cgataccgca tggacggcac gaccggaggc
     2401 cgcgcgttcg gcacggtcgc gtcgggtcgc ttccttgtcc gtagccgccc ccgctgcatg
     2461 ggcgcggaaa tcgccctggg cgatgggttc gagcaaggcc gccaactggt ccagcggcat
     2521 ctgcatgaac gcgccgatgt ccacgccggc gaaggtgacc gacagcgcct cgggcttgag
     2581 ccgcttgccg tggcaggtgg ggcacggttt gccctccatg aaccgggaca cgcgctttct
     2641 catcaacgcg ctctgggtgt tggcaaaggt gtgcagcacg tagcgcctgg cgccggtgaa
     2701 ggtgcccatg tagctcggct ccatcttgcg cttgagcgcg gcgcgggttt cggcgggcgt
     2761 gaagccggcg tacaccggca ccgtcggtgt ttcctcggtg aacagaatcc agtcgcgatc
     2821 cttcttcggc aggtccttcc acggtcggtc aacgtcgtag cccatgctga ccaggatgtc
     2881 gcgcaggttc tggccttgcc aggcgggggg ccaggaggcg atcgcccgct cgcggatgct
     2941 cagggaggga ttgggcacca tgatggcctc agtcacctcg tacacatggc ccaggccgtg
     3001 gcaggtcggg cacgctccct gcggcgtgtt gggcgaaaaa tcctcggcgt acagcatggg
     3061 ctggttggct gggtaggcgc cggcacgcga atacatcatc cgcaccaggc tggacaaggt
     3121 ggtcacactg cccacagagg aacgcgcgtt gctggagccc cgctgctgtt gcagcgccac
     3181 cgccggcggc aggccgtcga tcgcatcgac gtccggcacg ccggcctggt cgatcagtcg
     3241 ccgcgcatag ggggccaccg actcgaagta gcgccgctgg gcctccgcat agatggtgcc
     3301 gaaggccagc gaggacttcc cggagccgga gacacccgag aacaccacca gcgcgttacg
     3361 cgggatggag acgtccacct ccttgaggtt gttctcgcgc gcgccacgca cctgcacgag
     3421 gccgctggcc gcagcggtac aagaggattc accgaaagaa ttgtttggca tgttcttatc
     3481 ctgtagttcg tcccgctcag gacgggactt cctgagttcg ggtgccgcgg cggctggctt
     3541 caagcgctgc ggtgacagtg cgggcgtcgt agctgccacg cagacgacga ccctcgacga
     3601 agaacgtcgg cgtggcgtgc gcaccgctgg cgaccgcact ggcgaggtcg cgctcgacac
     3661 gctcgatcac cgcagggctg tcgagatccg cgatgaaccg atcgacgtcg agcccaagct
     3721 ctacggcgta gccgatcaga tcctcgcgct ccagcgcgtg ctgacgggtg aacacgaagt
     3781 ccagccacgg ccagaacatc ccctgattcg atgccgcctc ggacgcacgc gcggcgatcg
     3841 gcccatgcgg gtggtgcggc aactggcgca ccacataccg caggtcgtcc ccgaagtggg
     3901 cgcgcagatc ctcccaagaa ccggtagcgt gcgcacagta cgcgcactcg aagtccacgt
     3961 actccaccag cgtgagctgc gcgtcctctg gcccgcgaat gtgatcgacc tcgggatcga
     4021 ccggcggctc aagcaccatc ggcagatcgg cggtctcctc accccacctg cgagcggcaa
     4081 ccttgaagat gagccgcccg agcaacgtgg cgagcatcat cgacacgagc acgccgaccg
     4141 tcgcctggcg gcccaggtcg gaggtcgtgc cgaacgcgag cccgatgatg agcagcgaca
     4201 cggtgaaccc gatcccggac agtgccgcac caccgaacac gctccccatt ccgacacccg
     4261 ccggcaggcg ccccaggccg aggcggaccg cggcgagagt gatgagcccg atgcccagga
     4321 gcttgccgag cacgagcccc gcgacgacgc cccaggtcac gggcgagccg aaggcctcag
     4381 cgagcagccc accacgcagg tccaccccgg cgttcgccaa ggcgaacatc ggcacgatca
     4441 gcagcgctgt cggcagccgc aggaactcgt gcagccgctc gttcaccgag ataccccggg
     4501 acagcccgca gtccactgcg cgagccgatg cggcactcgg agactgccag aagtcacgga
     4561 acaactgtct tgccgcgacg accttgtgac gttgcgttgc gtaggcgggg atcagcagcc
     4621 ccgcggccat cccggcgagg gaggcatgga tgccggagta cacggtcgcg agccacagca
     4681 cgatcacgat cagcacatag ggcgttgccc gccactggcg gttgcgccct agcaaccaca
     4741 acccgacgag actggcgagg gcgatcagca acgggacgac ccggatctcc tcgctataga
     4801 cgatgccgat gatggacacg gcgaggaagt catcgaccac ggtcagggtg agcaagaaca
     4861 cgcgcaactg accggatagc cgcgggccga cgatcgccag ggtgcccagc atgaacgccg
     4921 tatcggtacc gaccaccgcc ccccacccgt gcaggccctc acttccggcc agccccacga
     4981 tcaggacata caccagcgcg ggaagcacga cacccgcgac ccccgcgatc agagcgagac
     5041 gggcacggct gcggtccctg agcgacccgt gggcgaattc ctgacgtacc tccaggccga
     5101 tcaggaagaa gaagacgacc atcaggccgt cgttgaccca gtgatgcaga tccatgtgca
     5161 ggcccagcgg cccgaagtca aacccgacgt ccaggtgcca caactcgaaa taggcgttgg
     5221 atagcggcga gttcgcccac gccagcgcca cgaccgtgac gatcaccagc agcaccgcag
     5281 caccagactc cgtgtgcaga tagcgggcaa ctcgtcttcg tcggatactc gccgaggatt
     5341 caccgagaga agcgtctggc atgttcttat cctgtagttc gtgaggtagc gatgggccga
     5401 tttcgggggg aggtcggtca agcgtccggg cttggcggcg tcgcgtcatc gagcgagcgc
     5461 agccaagtaa gcctttctcc cagccttcgc tcgacgcccc tgtccgttgg ctggtaccag
     5521 cccggccgcg ccacgccctc gggaaaatag gtttgcccag cggcataacc gttgggctca
     5581 tcatgcgcgt agcggtagcc ttcgtgatat cccatctgct tcatcagctt cgtcggcgca
     5641 ttgcgaagat gaagcggcac aggctgggag ccgctgcgct tgacgaatgc cttggcctga
     5701 tcccaggccg cataggtggc attcgacttg ggggccagaa cgaggtaagt caccgcctgg
     5761 gccagcggta tctcgccttc gggcgagccc agcctgtcgt aggccagcgc ggcattgagt
     5821 gccagttgca gcgcctgcgg gtcggcattg ccgatgtctt cgctggccat caccaccatg
     5881 cggcgggtca cttggctcac gtcgccgctg ccgtcgagga tgcgggccag ccaatacagg
     5941 gccgcatcgg ggtcggagcc ccgtagcgac ttgtggaagg ctgagagctg ccagtagaac
     6001 tcgtcaccgc ccttgtcgaa cctgcgcagc gatggactgg tggacagctt ggcgaactcg
     6061 ccatcgacca cggtcttgcc agcgcccgac gccgcattcg tcacctgctc aagcagattg
     6121 aggaagcgcc tgccatcgcc atcggcaaag ccctttagca ggtccagcgc gtccgtgtcc
     6181 aacgtgacgc cctggagatg cggcagtgcg cgttcgtaga gctggttcag ttcgtcgctg
     6241 gacagcggtt cgagggtata gacctgcgcg cgcgagagca gtgcggaatt gaccgccaac
     6301 cccggatgct cggtcgt
//
LOCUS       DATCUN010000005         3947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_93927, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000005 DATCUN010000000
VERSION     DATCUN010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93927"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="VIO83_00095"
     CDS             <1..157
                     /locus_tag="VIO83_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253599.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HEY8329724.1"
                     /translation="MLGGLLLGVAEAQFSGLVNTDYKDVFSFSLLVLILIFRPQGLLG
                     RPQVAKV"
     gene            160..1437
                     /gene="livM"
                     /locus_tag="VIO83_00100"
     CDS             160..1437
                     /gene="livM"
                     /locus_tag="VIO83_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HEY8329725.1"
                     /translation="MPVASPLNLRHALLDSLFAGLIALVVFGPIVGVVLDGYRFNLQP
                     VRVAWIVGTVMCGRLLLSLFLQTATGRSWMQRFEGSGAGVHVLAPEHRSRLRWIVPLV
                     LLAALVFPFFASKYLLTVVILGLIYVLLGLGLNIVVGLAGLLDLGYVAFYAIGAYGLA
                     LGYEYLGLGFWSVLPLAALLAALAGALLGFPVLRMHGDYLAIVTLGFGEIIRLVLNNW
                     LSFTGGPNGMPVPAPTLLGLEFGRRAKEGGVPIHEFLGIAYDPNLKFVFIYAVLLLTV
                     LLVLRVKGRLTRMPIGRAWEALREDEIACRALGLNHVLVKLSAFMLGASTAGLAGVFF
                     ASYQGFVNPSSFTFFESALILAIVVLGGMGSTLGVVIAAFVLTVAPELLRTFADYRVL
                     LFGMLMVLMMIWRPRGLIRISRRGFAERPGVAP"
     gene            1434..2309
                     /locus_tag="VIO83_00105"
     CDS             1434..2309
                     /locus_tag="VIO83_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8329726.1"
                     /translation="MTGEHVLSVAHLSMRFGGIRALHDVSLGVQRGSITALIGPNGAG
                     KTTLFNCLTGFYRASGGSIRLYTRGRATDVLQVLGEPFQLGDFVRPRQFASRLWYKLF
                     GGTHRVNRAGLARTFQNIRLFREMSVLENLLVAQHLWLNRNLLAGVLNTPGFRRAEEQ
                     ALAHAFYWLELVDLVDCANRLAGELSYGQQRRLEIARAMCTRPQLLCLDEPAAGLNPA
                     ETAALGAIIRTLRDTHQLSVLLIEHDMGMVMGISDHIVVLDHGEVIAAGPPAAIRSDP
                     KVIAAYLGADEEEPA"
     gene            2306..3022
                     /locus_tag="VIO83_00110"
     CDS             2306..3022
                     /locus_tag="VIO83_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8329727.1"
                     /translation="MSVPLLEFRDVDLCYGPVQALRGVSLQVEEGETVCLIGANGAGK
                     SSLLMSIFGQPRASAGQILLRGEDITGKSAHHVAASGVAQSPEGRRVFPDMSVEENLL
                     MGTITIGDRHADEDLRRMFELFPRLLERRNQRAMTLSGGEQQMLAIARALMSRPRLLL
                     LDEPSLGLAPLVVKQIFQILRELAAGGMTIFLVEQNANHALKLADRGYVLVNGEIRLS
                     GTGAELLGNPEVRSAYLGGH"
     gene            complement(3185..3631)
                     /locus_tag="VIO83_00115"
     CDS             complement(3185..3631)
                     /locus_tag="VIO83_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8329728.1"
                     /translation="MPTHTVRLHRVLRAPPERIYRAYLDADALVKWLAPHGFTAKVHQ
                     LEARVGGTYRMSFTNFSTGKSITFGGEYLELVANERIRNSDHFDDPNLPGTMQTTVSL
                     KGVSCGTELTIVQEGIPEAIPAESCYLGWQESLLLLAQLVEAEIPD"
     gene            complement(3671..>3947)
                     /locus_tag="VIO83_00120"
     CDS             complement(3671..>3947)
                     /locus_tag="VIO83_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329729.1"
                     /translation="VTPGNVVPSRELLGEMLLITNQPTQALAEFERSLKRDPNRLRGL
                     YGAARAAEASGNRQAAADYYRKLLDITAKRDTERSEVVQAKAYIEGQ"
BASE COUNT          535 a         1332 c         1337 g          743 t
ORIGIN      
        1 gatgctcggc ggcctgctgc tcggcgtcgc cgaggcgcag ttctccggcc tggtcaacac
       61 cgactacaag gacgtgttca gcttctcgct gctggtgctg atcctgatct tccgcccgca
      121 aggactcctg ggccggcccc aagtggcgaa ggtgtgagca tgccggtcgc gtctcccctg
      181 aacctccgtc acgccctgct ggacagcctg ttcgccggcc tgatcgccct ggtggtgttc
      241 ggccccatcg tcggcgtggt gctcgatggc taccgcttca acctgcagcc ggtgcgggtc
      301 gcctggatcg tcggcacggt gatgtgcggt cgcctgctgc tcagcctgtt cctgcagacg
      361 gcgacagggc ggagctggat gcagcgtttc gagggcagcg gcgcgggcgt ccatgtgctg
      421 gcgccggagc accgctcgcg gctgcgctgg atcgtgccgc tggtactgct ggcggcgctg
      481 gtcttcccct tctttgccag caagtacctg ctcaccgtgg tcatcctcgg cctgatctac
      541 gtgctgctcg gcctcggcct caacatcgtg gtcggcctcg ccgggctgct ggatctgggc
      601 tacgtggcct tctacgccat cggcgcctac ggtctggcgc tgggctacga gtacctgggt
      661 ctcggtttct ggagcgtgtt gccgctggcg gcactgctgg cggcgctggc cggggcgctg
      721 ctcggtttcc cggtgctgcg catgcatggc gactacctgg ccatcgtcac cctcggcttc
      781 ggcgagatca tccgcctggt gctgaacaac tggctgtcgt tcaccggcgg gccgaacggc
      841 atgccggtgc cggcgccgac cttgctcggt ctggagttcg gtaggcgggc gaaggagggc
      901 ggcgtgccga tccacgagtt cctcggcatt gcctacgatc ccaacctcaa gttcgtgttc
      961 atctacgcgg tgctgctgct caccgtgttg ctggtgctgc gcgtcaaggg ccggctgacg
     1021 cgcatgccga tcggccgcgc ctgggaagcg ctgcgcgagg acgagatcgc ctgccgggcg
     1081 ctgggcctca accacgtgct ggtcaagctc tcggcgttca tgctcggcgc ctccaccgcg
     1141 gggctggccg gggtgttctt cgccagctac cagggcttcg tcaacccgag ttcgttcacc
     1201 ttcttcgagt cggcgctgat cctcgccatc gtcgtgctcg gcggcatggg ctcgaccctc
     1261 ggcgtggtga tcgccgcctt cgtgctgacc gtggcgccgg agctgctgcg caccttcgcc
     1321 gactaccggg tgctgctgtt cggcatgctc atggtgctga tgatgatctg gcgcccgcgc
     1381 gggctgatcc gcatcagccg ccgcggcttt gccgagcgac cgggagtggc gccatgaccg
     1441 gggagcacgt gctcagcgtc gcccatctgt ccatgcgctt cggcggcatc cgcgcgctgc
     1501 acgacgtcag cctgggcgtg caacgcggct cgatcaccgc gctgatcggc cccaacggtg
     1561 ccggcaagac cacgctgttc aactgcttga ccggcttcta ccgcgccagc ggcggcagca
     1621 tccgcctgta tacccgcggc cgggccaccg acgtgctgca ggtgctcggt gagccgttcc
     1681 agctgggcga cttcgtgcgc ccgcggcagt tcgccagccg cctgtggtac aagctcttcg
     1741 gcggcaccca ccgggtcaac cgcgccgggc tggcgcgcac cttccagaac atccgcctgt
     1801 tccgcgagat gtcggtgctg gagaacctgc tggtggccca gcacctgtgg ctgaaccgca
     1861 acctgctggc cggggtgctc aataccccgg gctttcgccg tgccgaggag caggcgctgg
     1921 cccatgcctt ctactggctg gaactggtcg atctggtcga ctgcgccaac cgcctggccg
     1981 gtgagctgtc ctacggccag cagcgtcgcc tggagatcgc ccgcgccatg tgcacccgcc
     2041 cgcagttgct gtgtctggac gagccggccg ccggcctcaa tccggcggag accgccgcgc
     2101 tcggcgccat catccgtacc ctgcgcgaca cgcaccaact cagcgtgctg ctgatcgagc
     2161 acgacatggg catggtgatg ggcatttccg accacatcgt ggtgctcgac cacggcgagg
     2221 tgatcgccgc cgggccgccg gcagcgatcc gcagcgatcc caaggtgatc gccgcctatc
     2281 tgggcgccga cgaggaggag ccggcatgag cgtgccgctg ctagaatttc gcgatgtcga
     2341 cctctgctac ggcccggtgc aggcgctgcg cggggtcagc ctgcaggtgg aggagggcga
     2401 gacggtgtgc ctgatcggcg ccaacggcgc cggcaagtcg agcctgctga tgtcgatctt
     2461 cggccagccg cgcgccagcg ccgggcagat cctcctgcgc ggcgaggaca tcaccggcaa
     2521 gtctgcccac cacgtggccg ccagcggcgt ggcccagtcc cccgaggggc ggcgggtgtt
     2581 tccggacatg agcgtcgagg agaacctgct gatgggcacc atcaccatcg gcgaccgcca
     2641 cgccgacgag gacctgcggc gcatgttcga actgttcccg cgcctgctgg agcggcgcaa
     2701 ccagcgcgcc atgaccctct ccggcggcga gcagcagatg ctcgccatcg cccgcgcgct
     2761 gatgagccgg cccaggctgc tgctgctcga cgagccctca ttgggcctgg cgccgctggt
     2821 ggtcaaacag atcttccaga ttctccgcga gctggcggct ggcggcatga ccatcttcct
     2881 ggtcgagcag aacgccaacc acgcgctcaa gctggccgac cgcggctacg tgctggtcaa
     2941 cggcgagatc cgcctttccg gcacgggcgc cgaactgctg ggcaatcccg aggtgaggag
     3001 cgcctatttg ggcgggcact gacgggcagg ctgttgaaaa actacctgcg ttgccgatgc
     3061 tgcgttaaaa acggcccggg acacggcgtc tcaaaatgct catttacagc acgtaaactg
     3121 cgcttttccg gccgtttttg ccttgcctcg gctgcctcgc cgacgttttt caacagcctg
     3181 ctagctaatc gggaatctcc gcctcgacca gctgcgccag cagcaggagc gattcctgcc
     3241 agccgagata gcaggattcc gccgggatgg cctcgggtat cccctcttgc acaatggtca
     3301 gctcggtccc gcaagacacg cccttcaggg agaccgtcgt ctgcatggtg ccgggcaggt
     3361 tcggatcgtc gaagtggtcg ctgttgcgga tgcgttcgtt ggctaccagc tcgaggtatt
     3421 cgccgccgaa ggtaatgctt ttcccggtgc tgaagttggt gaatgacatc cggtaggtgc
     3481 cgccgacccg ggcctccagc tgatgcacct tggcggtgaa accgtgcggc gccagccact
     3541 tgaccagggc gtcggcgtcc agataggcgc gatagattcg ctcgggcggc gcacgcagta
     3601 cgcggtgcag gcggacggtg tgggtcggca tggggctttt tcctgtcgtt ggcgatggct
     3661 tgtcggcggg ttactggccc tcgatgtacg ccttggcctg cacgacttcg ctacgctcgg
     3721 tgtcgcgctt ggcggtgata tccagcagct tgcggtagta gtccgccgcc gcctggcggt
     3781 tgccgctggc ctctgccgcg cgggcagcgc cgtacaggcc gcgcaggcga ttgggatcgc
     3841 gcttgagcga gcgttcgaac tccgccagcg cctgggtcgg ctggttggtg atgagcagca
     3901 tctcgcccag cagctcgcgc gagggcacca cgtttcccgg cgtgacg
//
LOCUS       DATCUN010000006         5970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_93942, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000006 DATCUN010000000
VERSION     DATCUN010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93942"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1748
                     /locus_tag="VIO83_00125"
     CDS             <1..1748
                     /locus_tag="VIO83_00125"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEY8329730.1"
                     /translation="SKPAEPTPAPAAAPAAQPAAAPAAAKPAAVKPAAVKPVATPVEV
                     YEPGLLDEVLANPLLLAAAGGALALLAGLGLTINSRRKSRKSAEQEAELFQQPDLSAV
                     QPDFTPVAGLAAQADGVQSPAALTTGLQSSDPLGEAEMYSTYGRFNQAAELLRNALND
                     EPQRADLRLKLMEVCAEMGDREGFVRQEAELGEIGGAQPQIDQLRARYPQILASGAAI
                     AAGTALAADVFDSEPEVAAPEPVAAARVEPADEFSFDDFSLDDLSLDAVPAAAPSAAA
                     EPVSALDDGFDLSLDDLEAELERELAANPAAAVEAPLQLDELSLDGLDDDFASLEPAP
                     AAEPLLEGGELDFELDLDLAAAPAGGEVAQPSAEVDDFAFELGEVQLPVVDEVLAAPQ
                     DQTEEDELLAELDQLALDAPAAPATAESDEFLLDLADLDLGESSAAPAAQAEAELAAL
                     PGLDDLELELQTLSGADAEQDLDAAFDDFLTRSELPEASAAPAPVAAVETLDELPALD
                     ELAFETTPAPALTGNEDFDFLADADETATKLDLARAYIDMGDADGARDILDEVLKEGS
                     ASQQQEAREMMGRIA"
     gene            1751..2602
                     /gene="truA"
                     /locus_tag="VIO83_00130"
     CDS             1751..2602
                     /gene="truA"
                     /locus_tag="VIO83_00130"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY8329731.1"
                     /translation="MTLQPVVPAAAEVAAAGVSRIALGVEYKGARYRGWQRQETGVPT
                     VQEVLEKALSKVAAEPVSLHCAGRTDASVHASGQVVHFDTRVERPLIAWVMGTNANLP
                     GDISVTWAQPMPAHFHARFSAVARRYRYVIYNDPVRPAHLAEEITWNHRPLDVARMRE
                     AAQALVGTHDFTSFRALKCQAKSPVKTLHHLRVIEQGRFIVLDIRANAFLHHMVRNIA
                     GVLMTIGAGEREPSWTGEILAARDRRSGGVTAHPYGLYLIQVEYPEEFQLPQRYLGPH
                     FLSAMDD"
     gene            2705..3325
                     /locus_tag="VIO83_00135"
     CDS             2705..3325
                     /locus_tag="VIO83_00135"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY8329732.1"
                     /translation="MPVVRSKICGITRIEDALLAAEAGADAIGLVFYGKSPRAVDVRQ
                     ARAIVAALPPFVTSVGLFVNASRCELAEILEVVPLDLLQFHGDETAADCAGYHRPYIK
                     ALRVRPGEDVKARCAEFPQAAGILLDTFVPGVPGGTGEVFDWSLVPQDPGCPIILAGG
                     LTPENVGAAIRQVRPWAVDVSGGVEASKGIKDAGKVRAFVEAVRAS"
     gene            3590..4486
                     /gene="accD"
                     /locus_tag="VIO83_00140"
     CDS             3590..4486
                     /gene="accD"
                     /locus_tag="VIO83_00140"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY8329733.1"
                     /translation="MSNWLVDKLIPSIMRSEVKKSSVPEGLWHKCPSCDAVLYRPELE
                     KTLDVCPKCDHHMRIDARARLNFFLDAEGREEIAAELEPVDRLKFRDSKKYKDRLTAA
                     QKDTGEKDALVAMSGTLQGLAVVACAFEFSFMGGSMGSVVGERFVRAANVALEKRCPL
                     ICFAASGGARMQEALISLMQMAKTSAVIARLREEGIPFVSVLTDPVYGGVSASLAMLG
                     DVNVAEPKALIGFAGPRVIEQTVREKLPEGFQRSEFLIEHGAIDMIVHRAELRPRLAR
                     LLAQLTHQPTPAAAPAAELLGA"
     gene            4483..5790
                     /gene="folC"
                     /locus_tag="VIO83_00145"
     CDS             4483..5790
                     /gene="folC"
                     /locus_tag="VIO83_00145"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEY8329734.1"
                     /translation="MSRSLADWLSYLEQLHPVSIDMGLERARLVQQRLGLGRPAPRVI
                     TVTGTNGKGSTCAFLASLLRASGLRVGVYSSPHLLRYNERVLLPDGEASDAALCEAFA
                     AVEAARGETSLTYFEMGTLAAFWLFERAALDAVVLEVGLGGRLDAVNLVDADLAVVTS
                     IGVDHVEWLGDSRSSVAVEKAGIFRAGKPVLCGDLDPPPVLLEQARQLGCPLLLRGRD
                     FDLAYGEQGWHWRGVDAGGQPLELTNLPLLDLPMENAALALQAFARLGLPWQAPTLVA
                     ALQQAHLAGRLDRRTLCWQGRPLNLLLDVGHNPHAAQYLAQRLAQRPPRGRRLAVFGL
                     LGDKDLAGVIEPLQGLVAHWAVAPLPTPRSRSAEELLAALQARSASADAWPSVAAAIE
                     AQAIRATADDEILVFGSFYSVADALYWLEQHGQSGSKRQDNQG"
     gene            5806..>5970
                     /locus_tag="VIO83_00150"
     CDS             5806..>5970
                     /locus_tag="VIO83_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY8329735.1"
                     /translation="MALLDRRFKQRIVGAAVLVVLVVVFLPMLFNREDEQRRVRVEAP
                     PMPSAPPVVVA"
BASE COUNT          876 a         1941 c         2101 g         1052 t
ORIGIN      
        1 agagcaagcc ggccgagccg actcctgcgc ctgccgcagc tccggctgcc cagccggccg
       61 ctgcgccggc agccgccaag cctgctgccg tcaagcctgc tgccgtcaag ccggttgcca
      121 ctccggtgga ggtctacgag cctggcctgc tcgacgaagt gctggccaat ccgctgttgc
      181 tggccgccgc tggcggtgcc ctggctctgc tggccgggtt gggcctgacg atcaattcgc
      241 gccgcaagtc gcggaagtcg gccgagcagg aggccgagct gttccagcag ccggacctgt
      301 ctgccgtgca acccgacttc actccggttg ccggtctggc ggcgcaggcg gatggcgtgc
      361 agagtccggc agcgctgacg accgggctgc aaagttccga tcccctgggc gaggccgaga
      421 tgtacagtac ctacggccgc ttcaaccagg ccgccgagtt gctgcgtaat gcgctgaacg
      481 acgagccgca gcgtgccgac ctgcgcctga agctgatgga ggtctgcgcc gagatgggcg
      541 accgtgaagg tttcgtccgt caggaggccg agctgggcga gatcggtggt gcccagccgc
      601 agatcgacca gctgcgtgcc cgctatccgc agattctcgc cagcggtgca gccatcgctg
      661 ccggtactgc cttggcggca gacgtcttcg atagcgagcc tgaagtggca gcgcccgagc
      721 ctgtagcggc tgcccgggtc gagccggccg atgagttttc cttcgatgac ttctcgctcg
      781 atgacctgtc tctcgatgcc gttccggccg ctgcgccgtc ggctgccgcc gagcctgtct
      841 ctgcactgga cgacggcttc gatctgagtc tcgacgatct ggaagccgag ctcgagcgcg
      901 agctggcggc caatccggcg gcggctgtcg aggctccgct gcagctcgac gagctgagcc
      961 tggatggtct ggatgatgat ttcgccagtc tcgagccggc gcctgccgcc gaacccctgc
     1021 tggagggtgg cgagctcgat ttcgagctgg acctggatct ggctgctgca ccggctggcg
     1081 gggaagtagc gcagccgtcc gccgaagttg acgactttgc cttcgagctg ggcgaggtgc
     1141 agctgccggt agtcgacgaa gtgctggctg ccccgcaaga ccagactgaa gaggacgagc
     1201 tgctggccga actggaccag ctggcgctgg acgctcccgc tgcgccggcg actgccgagt
     1261 ccgacgagtt cctgctggac ctggccgatc tggatcttgg cgagtcgtcc gctgcgccag
     1321 ctgcccaggc tgaggctgag ctggccgcgt tgccgggctt ggacgacctg gagctggagc
     1381 tgcagaccct gagcggcgcc gatgccgagc aggatctgga tgccgcgttc gacgacttcc
     1441 ttacccgctc cgagctgcct gaagcgagtg ctgcgcctgc accggtggcg gcggtcgaga
     1501 cgctggacga gctgccggcg ctggatgagc tggccttcga aacgaccccg gctcctgcgc
     1561 tgaccggcaa cgaggatttc gacttcctcg ccgatgctga cgaaaccgcc accaagctgg
     1621 acttggcccg tgcctacatc gacatgggcg atgccgatgg cgcccgcgac atcctcgacg
     1681 aggtgctcaa ggaaggctcc gccagtcagc agcaggaagc gcgcgagatg atgggtcgta
     1741 tcgcctgatc atgacgctcc agccagtagt gcctgcggca gccgaagtgg ctgccgcagg
     1801 cgtttccaga atcgccctcg gcgtcgaata caagggtgcc cgctaccgtg gctggcagcg
     1861 ccaggagact ggcgtgccga ccgtgcagga agtgcttgag aaggccctgt cgaaggtggc
     1921 tgccgaacct gtttccctgc actgtgccgg acgtaccgat gcctcggtgc atgccagtgg
     1981 ccaggtggtg catttcgata cccgcgtcga acgcccgctc atcgcctggg tcatggggac
     2041 caatgccaat ctgccgggcg atatcagcgt gacctgggcg cagccgatgc cggcgcattt
     2101 ccatgcgcgc ttttcggccg tggcgcggcg ctatcgctat gtgatctaca acgatccggt
     2161 gcggccggcg catctggccg aggaaatcac ctggaatcac cggccgctgg atgtggcgcg
     2221 catgcgtgag gcggcgcagg cactggtcgg cacccacgac ttcacctcgt tccgcgccct
     2281 gaagtgccag gccaagtcgc cggtcaagac cctccaccac ctgcgggtga tcgagcaggg
     2341 gcgcttcatc gtcctggaca tccgtgccaa tgccttcctg catcacatgg tgcgcaacat
     2401 cgccggagtg ctgatgacca tcggtgccgg tgagcgggaa ccctcgtgga cgggcgagat
     2461 tctcgccgcg cgcgaccggc gcagcggtgg ggtcactgct catccctacg ggctgtatct
     2521 gatccaggtc gagtaccccg aggagttcca gttgccgcag cgctatctgg ggccgcattt
     2581 cctctcggcg atggacgact gagcggcggc tgcgtggcgc tgcggtggca ggctgacgac
     2641 gctctgggca tttgttacca tcgcggcttt tccatcctga ctttgttcgc aaggggagcg
     2701 tgcgttgcca gtcgttcgca gcaagatctg cgggattacc cgcattgagg acgccctgct
     2761 cgcggccgag gccggcgccg acgccatcgg tctggtgttc tacggcaaaa gcccgcgggc
     2821 agtcgatgtg cgtcaggcaa gggcgatcgt tgccgcgctg ccgcccttcg tcaccagcgt
     2881 aggcctgttc gtcaatgcct cgcgctgcga gttggcggag atcctcgagg tcgtgccctt
     2941 ggatctgctg cagttccatg gcgatgaaac ggcggccgac tgtgccggct atcaccgacc
     3001 gtacatcaag gccctgcgcg tgcgtcccgg cgaggacgtc aaggcccgct gtgccgagtt
     3061 cccgcaggcc gcggggattc tcctggatac cttcgtcccc ggcgtgccgg gtggtaccgg
     3121 tgaggtcttc gactggtcgc tggtgccgca ggatccgggc tgcccgatca tcctcgcggg
     3181 tggcctgacc ccggaaaatg tcggtgcagc gatccgtcag gtgcgtcctt gggcggtgga
     3241 tgtcagcggc ggggtggagg caagcaaggg catcaaggat gcgggcaagg tgcgcgcctt
     3301 cgtcgaggcg gtgcgcgcca gctgagtacg ctcatgtgac ggccgccgcc gcgctgccgt
     3361 ccattagcct gatgttgccg caggcggttg cctggtcggc ggagagttta tcgcgtgacg
     3421 gcgctgcggc gaccgcgcgc accatggtcg cgagcgatgc gtaacccctc gtgccggcag
     3481 cagggcttgc cgggcgagcc gcgtcggact gcggcccaat cgtgaatctg ctcgaggacc
     3541 tgcgcggcgc acgcggtgcg cgccagtccg ggcctacgga gaacacagca tgagcaactg
     3601 gctggtagac aaactgatcc cctcgatcat gcgttccgag gtgaagaaaa gctcggtgcc
     3661 cgaggggctc tggcacaagt gcccgtcctg cgacgcggtg ctgtaccgtc ccgaactgga
     3721 aaagaccctg gatgtctgcc ccaagtgcga ccaccacatg cgcatcgacg cgcgtgcgcg
     3781 gctgaacttc tttctcgacg ccgagggccg cgaggagatc gccgccgagc tggagccggt
     3841 ggatcgcctg aagtttcgcg acagcaagaa gtacaaggat cgcctgaccg ctgcgcagaa
     3901 ggataccggc gagaaggatg cgctggtggc catgagcggt accctgcagg gactggcggt
     3961 ggtggcctgc gccttcgagt tctccttcat gggcggctcg atgggttccg tggtcggcga
     4021 gcgcttcgtg cgggcggcca atgttgccct ggaaaagcgc tgcccgctga tctgcttcgc
     4081 cgcctcgggt ggcgcacgca tgcaggaagc gctgatttcc ctgatgcaga tggccaagac
     4141 ctccgcggtc atcgcccgtc tgcgtgaaga aggcattccc ttcgtctccg tactgaccga
     4201 tccggtctac ggcggcgtct ccgccagcct ggcgatgctc ggtgacgtca acgtcgccga
     4261 acccaaggcg ctgatcggct tcgccggtcc gcgcgtgatc gagcagaccg tgcgtgagaa
     4321 gctgccggaa ggcttccagc gcagcgagtt cctgatcgag catggtgcca tcgacatgat
     4381 cgtgcatcgt gccgagctgc gtccgcgcct ggctcgcctg ctggcgcagc tgactcacca
     4441 gccgacgccg gccgccgctc cggccgcaga actgctcggc gcatgagccg cagtctcgcc
     4501 gactggctga gctatctcga gcagttgcac cccgtgtcca tcgacatggg gttggaacgc
     4561 gcccgcctgg tacagcagcg tctcggcctc ggccgcccgg cgccgcgggt gatcacggtc
     4621 accggcacca atggcaaggg ctcgacctgc gcctttctcg ccagcctgct gcgcgccagc
     4681 gggctgcgcg tcggcgtgta cagctcgccg cacctgctgc gctacaacga gcgggtgctg
     4741 ctgccggacg gcgaggccag cgatgctgcg ctgtgcgaag ccttcgccgc cgtcgaggcg
     4801 gcgcgtggcg agacctccct gacctatttc gagatgggta ccttggcggc cttctggctg
     4861 ttcgagcgcg cggcgctgga tgccgtggtg ctcgaggtcg gcctgggcgg tcgtctcgat
     4921 gcggtcaacc tggtggatgc cgatctggcg gtggtgacca gcatcggcgt cgaccacgtc
     4981 gagtggctgg gcgacagccg cagcagtgtg gcggtggaga aggccgggat cttccgtgcc
     5041 ggcaagccgg tgctgtgcgg cgatctcgac ccgccgccgg tgctgctgga acaggcgcgc
     5101 cagctgggct gtcccctgct gctgcgcggt cgcgatttcg atctggccta cggcgagcag
     5161 ggctggcact ggcgtggcgt ggatgccggc ggtcagccgc tggagctcac caatctgccg
     5221 ctgctcgatc tgccgatgga gaacgcggcc ctggcgctgc aggcctttgc ccggctgggg
     5281 ctgccctggc aggcgccgac cctggttgct gcgctgcagc aggcgcacct tgccggccgt
     5341 ctggatcgcc gcacgctatg ctggcaggga cgcccgctga acctgctgct ggatgtcggc
     5401 cacaacccgc atgcggcaca gtatctggct cagcgcctgg ctcagcgccc gccgcgcggc
     5461 cggcgcctgg cggtcttcgg cctgctgggc gacaaggatc tggccggggt catcgaaccg
     5521 ctgcaggggc tggtcgcgca ctgggccgtg gcgccgctgc cgaccccgcg cagtcgcagt
     5581 gccgaggagt tgctggcggc cctgcaggcg cgcagcgcca gtgccgacgc ctggccgtcg
     5641 gtggcggcgg ccatcgaggc tcaggccatc cgggcgacgg cggatgacga gattctcgtg
     5701 ttcggctcgt tctacagcgt ggctgatgcc ctgtactggc tggagcagca tggccagtct
     5761 ggcagcaagc gccaggacaa tcagggataa gacggagggg aagccatggc cttgctggac
     5821 cgaagattca agcagcggat tgtcggggct gccgtgctgg tggtgctggt ggtggtgttc
     5881 ctgccgatgc tgttcaatcg cgaggacgag cagcgccgcg tgcgcgtcga ggcgccgccg
     5941 atgccgtcag cgccgccggt ggtcgtggcc
//
LOCUS       DATCUN010000007         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_112706, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000007 DATCUN010000000
VERSION     DATCUN010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112706"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VIO83_00155"
     CDS             <1..801
                     /locus_tag="VIO83_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004881213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8329736.1"
                     /translation="RHPDRGGRLLLVLLPFALLLFAYFIGSAQRQADNPNDKLLPSAT
                     QMVVAVDRLAFTADKRSGDYLFWQDSGASLARLGMGIGIAALAGLCLGIAAGSLPLFG
                     APLSPLLTVLSMVPPLAILPILFIVFGLGELAKVMLIVIGITPVLARDLEQQARQIPA
                     ELLIKAQTLGASTWTLILRVVLPQLLPRLLIALRLVLGSAWLFLIAAEAIAATDGLGY
                     RIFLVRRYMAMDVILPYVLWITLLAWLMDLGLRQLTRLAFPWYEGAKA"
     gene            798..1733
                     /locus_tag="VIO83_00160"
     CDS             798..1733
                     /locus_tag="VIO83_00160"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8329737.1"
                     /translation="MSNKKLGADASESLARRPEHSNRNGHVVHEDCAHRATQPATRAA
                     GAFLEVRNVWQEYADQVVIERLNLKVAEGEFCTLVGASGCGKSTFLRLLLGQERPSRG
                     EILLDGQPLAGEPDASRGIVFQRYTVFPHLSVLDNVALGLELPRSKLLGRLFGSAKRE
                     AREQAAEMLRKVGLGHALDKYATQLSGGMQQRLAIAQALVMKPRVLLLDEPFGALDPG
                     IRKDMHALLLELWRETNLTVFMVTHDLSEGFSLGTRLLVFDKVRVDPHAPNAYGARVT
                     YDIPLNSDRRAARAAVDALPAHIGGLPSALQGVTP"
     gene            1730..2458
                     /locus_tag="VIO83_00165"
     CDS             1730..2458
                     /locus_tag="VIO83_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP1"
                     /protein_id="HEY8329738.1"
                     /translation="MTASLTLRPTLYEELVPGGGHTSFVLKRGQLLRITDLEGGANVS
                     LLLLNAAEKSERLNLPDTLKCQHTAKLTAGHCLYSDMGRVLAAITADTCGWHDSFGGV
                     LNAEEVREKYGAGRYQELRNGFYRNGVDNLLVEMGKWNLNLQDLLMTLNLFSKVTVDE
                     GGGFQFESGNSRAGDYVELYAPMDCLVVLTALQHPQDPNPVYSPKPVQLTWHRVDSDG
                     ISVLCRTSRPENGRGFHNTERLYI"
BASE COUNT          397 a          913 c          767 g          385 t
ORIGIN      
        1 cgacaccccg accgtggtgg ccgcctgctg ctggtgctgc tgccgttcgc cctgctgctg
       61 ttcgcctact tcatcggctc ggcgcagcgc caggcggaca accccaacga caagctgctg
      121 cccagcgcca cgcagatggt cgtcgccgtc gaccgcctgg ccttcaccgc ggacaagcgc
      181 agcggcgact acctgttctg gcaggacagc ggcgccagcc tggcgcgcct cggcatgggc
      241 atcggcatcg ccgcgctggc cggcctgtgc ctggggatag ccgccggcag cctgccgctg
      301 ttcggtgcgc cgctgtcacc gctgctcacc gtgctgtcga tggtgccgcc gctggcgatc
      361 ctgccgatcc tgttcatcgt cttcggcctc ggcgaactgg ccaaggtgat gctgatcgtt
      421 atcggcatta ctcccgtctt ggcccgcgac ctagagcagc aggcccggca gataccggcc
      481 gagctgctga tcaaggcgca gaccctcggc gccagcacct ggacgctgat cctgcgggtg
      541 gtgctgccgc aactgctgcc gcgcctgctg atcgccctgc gcctggtgct cggctcggcc
      601 tggctgttcc tgatcgccgc cgaggccatc gccgccaccg acggcctcgg ctaccggatc
      661 ttcctggtgc gccgctacat ggccatggac gtgatcctgc cctacgtgct gtggatcacc
      721 ctgctcgcct ggctgatgga tctcggcctg cgccagctca cccggctggc cttcccctgg
      781 tatgaaggag ccaaggcatg agtaataaaa aacttggcgc cgacgcgagc gagtcgctgg
      841 caaggcgccc ggagcacagc aaccggaatg gacacgtagt ccatgaggat tgcgcgcacc
      901 gcgcaacgca gccagcgact cgcgcagccg gcgccttcct cgaagtgcgt aacgtctggc
      961 aggaatacgc cgatcaggtg gtcatcgagc gtctcaacct gaaggtggcc gagggcgaat
     1021 tctgcaccct ggtcggcgcc tccggctgcg gcaagtcgac cttcctgcgc ctgctgctcg
     1081 gccaggagcg tcccagccgc ggcgagatcc tcctcgacgg tcagccgctg gccggcgagc
     1141 cggacgccag ccgcggcatc gtgttccagc gctacacggt gttcccgcac ctgtcggtgc
     1201 tcgacaacgt cgccctcggc ctggaactgc cgcgctcgaa gctgctcggc cgcctgttcg
     1261 gcagcgccaa gcgcgaggcc cgcgagcagg ccgccgagat gctcaggaaa gtcggcctcg
     1321 gccatgcgct ggacaagtac gccacccagc tctccggcgg catgcagcag cgcctggcca
     1381 tcgcccaggc gctggtgatg aagccgcgcg tgctgctgct cgacgaaccg ttcggcgccc
     1441 tcgatcccgg catccgcaag gacatgcacg ccctgctgct ggagctgtgg cgcgagacca
     1501 acctgaccgt attcatggtc acccacgacc tctccgaagg cttcagcctc ggcacgcgcc
     1561 tgctggtgtt cgacaaggtg cgcgtcgacc cgcacgcgcc caacgcctac ggcgcgcggg
     1621 tgacctacga catccccctg aacagcgacc gccgcgccgc gcgcgccgcc gtcgacgccc
     1681 tgcccgcgca tataggcgga ctgccatccg cgctccaagg agtaacccca tgaccgcatc
     1741 cctgaccctg cgcccgaccc tgtacgagga actcgtcccc ggcggcgggc acacctcctt
     1801 cgtgctcaag cgcggccagc tgctgcgcat caccgacctc gagggcggcg ccaacgtcag
     1861 cctgctgctg ctcaacgctg ccgagaaaag cgagcgcctc aacctgccgg acaccctcaa
     1921 gtgccagcac accgccaagc tcaccgccgg ccactgcctg tactcggaca tgggccgcgt
     1981 gctcgccgcg atcaccgccg acacctgcgg ctggcacgac agcttcggcg gcgtgctgaa
     2041 tgccgaggaa gtgcgcgaaa aatacggcgc cggccgctac caggagctgc gcaacggctt
     2101 ctaccgcaac ggcgtggaca acctgctggt cgagatgggc aagtggaacc tcaacctgca
     2161 ggacctgctg atgaccctca acctgttcag caaggtcacg gtcgacgaag gcggtggctt
     2221 ccagttcgaa agcggcaact cgcgcgccgg cgactacgtc gagctgtacg cgccgatgga
     2281 ctgcctggtg gtgctcaccg ccctgcagca cccgcaggac cccaacccgg tctactcgcc
     2341 caagccggtg cagctcacct ggcaccgcgt cgacagcgac ggcatcagcg tgctgtgccg
     2401 cacctcgcgt ccggaaaacg gccgcggctt ccacaacacc gaacgtctgt atatctgagg
     2461 ag
//
LOCUS       DATCUN010000008        10164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_75178, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000008 DATCUN010000000
VERSION     DATCUN010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10164
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75178"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VIO83_00170"
     CDS             <1..190
                     /locus_tag="VIO83_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014337586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY8329739.1"
                     /translation="LEAVAREAASKANLQPEFIKVTDMMEIMAYDLLSTPALVINEKV
                     VSSGRIPTVAEVQQWLTA"
     gene            674..1030
                     /locus_tag="VIO83_00175"
     CDS             674..1030
                     /locus_tag="VIO83_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8329740.1"
                     /translation="MVEHLTPTTVFKCLADETRARMTLLIAREGELCVCELTAALEES
                     QPKISRHLALLRSSGVLEDRRQGQWVYYRLHPHLPDWVHDMLKTILAANKNWLSPEVQ
                     RLEGMRNRPERVGACG"
     gene            1046..1516
                     /locus_tag="VIO83_00180"
     CDS             1046..1516
                     /locus_tag="VIO83_00180"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019752755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY8329741.1"
                     /translation="MKVLFLCTANSCRSILCEALFNHLAPAGLRAFSAGSQPKGFVHP
                     LTLEALARAGVPGEGLASKPSETHADLQPDLVITVCDKAAGEACPVFFGPALKAHWGL
                     ADPSEVEGSDSEIAVAFDATVARITARTQAFLALPLDQLDADALKAELARIASL"
     gene            1540..2253
                     /gene="arsH"
                     /locus_tag="VIO83_00185"
     CDS             1540..2253
                     /gene="arsH"
                     /locus_tag="VIO83_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical resistance protein ArsH"
                     /protein_id="HEY8329742.1"
                     /translation="MYDDLPNIDPDLLDLPDLGKLGLPAQPEHKPRILLLYGSNRERS
                     YSRLLVQEAARLLERFGAETRIFDPSGLPLPDDAPESHPKVQELRELVLWSEGQVWCS
                     PERHGAMSAVFKAQIDWIPLTMGAIRPSQGKTLALMQVCGGSQSFNAVNQMRVLGRWM
                     RMFTIPNQSSVAKAFLEFDEEGRMKPSSYYDRVVDVMEELVKFTLLLRGRADYLVDRY
                     SERKESAEELSRRVNQRAI"
     gene            2279..2776
                     /locus_tag="VIO83_00190"
     CDS             2279..2776
                     /locus_tag="VIO83_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclin-dependent kinase inhibitor 3 family
                     protein"
                     /protein_id="HEY8329743.1"
                     /translation="MQHPYDILGVPGCSGRLIFTPCPGSKDTSIASALDTLKAAGAEA
                     LITLMPASELEQNQAAELPALCAARNLEWFHLPVADEQVPLDDFASAWAQARERIRVL
                     LDEGRSIAIHCKGGSGRTGLIAARILIDRGLSRAEAVALVQALRPKAIQHPAHLAWIE
                     QFDAA"
     gene            2805..3809
                     /locus_tag="VIO83_00195"
     CDS             2805..3809
                     /locus_tag="VIO83_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsJ-associated glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY8329744.1"
                     /translation="MTIKVGINGFGRIGRLALRAAWDWPELEFVQINDPAADAATHAH
                     LLNFDSVHGRWQHEAGSDGDCIVIDGKRIKVTHNRAIADTDWSGCDLVIEASGKMTKV
                     ALLQAYLDQGVKRVVVSAPVKEEGALNIVMGVNDQLFDPAKHRIVTAASCTTNCLAPV
                     VKVIHEALGIRHGSITTIHDLTNTQSLLDQPHKDLRRARASLMSLIPTTTGSATAIAE
                     IFPELRGKLNGHAVRVPLANASLTDCVFEVERATTAEEVNALLKAAAEGPLKGILGYE
                     ERPLVSIDYRTDPRSSIIDALSTLVVNGTQVKIYAWYDNEWGYANRTVELARKVGLPG
                     "
     gene            4010..5239
                     /gene="arsJ"
                     /locus_tag="VIO83_00200"
     CDS             4010..5239
                     /gene="arsJ"
                     /locus_tag="VIO83_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004575548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organoarsenical effux MFS transporter ArsJ"
                     /protein_id="HEY8329745.1"
                     /translation="MHALSRLSPEIRQYLLVTGNYWAFTLTDGALRMLVVLHFHGLGY
                     SPLQIAALFLFYEIFGVVTNLVGGWLGAHLGLNRTMNIGLGLQVAALLMLTVPAAMLT
                     VPWVMAAQALSGIAKDLNKMSAKSSIKLLVADGQQGTLYKWVALLTGSKNALKGVGFF
                     LGGALLTGLGFTGAVLAMAAVLALIWLASLVTLKKDLGKAKSKPKFRDLLSKSRAINI
                     LSAARLFLFGARDVWFVVALPVYLASTFGWDFWKVGGFLAIWVIGYGIVQSFAPYFTG
                     KRSGKLPDGRAAFVWAALLAGLPAAIALGLGTELSAQTVLIGGLLLFGALFAINSSLH
                     SYLIVSYAKEDGVSLDVGFYYMSNALGRLIGTLLSGWVYQAWGLEACLWVSSAFVLLA
                     ALISIGLPRHAPIAQAS"
     gene            5305..6225
                     /locus_tag="VIO83_00205"
     CDS             5305..6225
                     /locus_tag="VIO83_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY8329746.1"
                     /translation="MAHAHHHDHHSHAHAPQRYDRAFAIGIVLNLGFVLVEAFYGWQV
                     NSLALLADAGHNLSDVGGLILAWAALAATRLRPNDRHTYGWQRGSILASFTNASILLV
                     AMGSLGWESIQRLQQPVAIVGETVMVVAGIGVLVNAATAALFLSGSRSDLNLRGAFLH
                     MAADALVSIGVVLAGGLYLWQGWSWIDPLVSLAIAVVIVLGTWSLFRQSLHLLFDGVP
                     EHVDLPAVRQYLLEQPGVCDVHDLHVWAMSTAEVALTAHMAMNPLPEDDRFLQGIAHE
                     LHEHFGISHATLQLERDLGCPLGCARLPCR"
     gene            complement(6235..6486)
                     /locus_tag="VIO83_00210"
     CDS             complement(6235..6486)
                     /locus_tag="VIO83_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329747.1"
                     /translation="MSRDVRKLQIVNRLGGARALITYMDSNRVETAKTRPAEKTEVLA
                     DFRPHGFIVLLGYDEDLWRHPDTGAQYPHAPNAWAYCPE"
     gene            6813..8957
                     /locus_tag="VIO83_00215"
     CDS             6813..8957
                     /locus_tag="VIO83_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8329748.1"
                     /translation="MSIARRFALPTLLPCLLASPAGQAADLFLDGEQLPEVLTATRLK
                     QSPAAVPGSVTVLDRALIQATGARDIPELMRLVPGMMVGYTKGNLSTVNYHGGRVSEA
                     RRLQVLVDGRSVYRPGLATVDWTDLPLAIEDIERIEVFRGPNTVSYGANALAGVISIT
                     TRRPADSQGTRLKYTRGERGVDDWYASEGFHAGSGDFRLSLSGQEDNGFHHTDEGEKY
                     RDSRRSTRFQLSASHVLNDEQSLDWQVAAKEGSNQRNYYSDEEALIRNVVPRGDTDKD
                     VVARDYAGSVRWSLDASADHNVQVQASAQRWERRQEWRACDLALSFTPEVSQLFALNP
                     DSVDTLARLLRKGRGDAVETLLKGQERALARVIRDNYTGPDGAALRAPVCGDINNNID
                     ETRFDLEVQDTLSLTDNLRLLSGASYRRDISSSETYFDGRLSNDIWRLFGHLEWYLNE
                     HIVLQGGAMYEQDKFSGDSLTPRVALNYLITPRHGLRFVYSEAVRSPDMFEDRVQWRY
                     RMENLSGDAAGRDSAYYYAYANGPKNLDQEIMRSREIGYNGQFDFGLRIDIRVFHEEI
                     HQMISEPLDITEFRPSNDNQMWFRGAETEIDWQIDSADRLRLTYAYVDFAATAKIDRR
                     LTARYSGSGGWMRDWGRGWSSSLFYYGADLLNERRFERLDLRLAKRLRLGATELELAG
                     VLQQRLDDEPLTWRENLYDDRRQLYFSAQLEF"
     gene            complement(9091..9450)
                     /locus_tag="VIO83_00220"
     CDS             complement(9091..9450)
                     /locus_tag="VIO83_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8329749.1"
                     /translation="MKVLLVDDTAFNRTLPRVLLQRYGCSVVECDNGTDALRMASSEQ
                     FDCVLLDIMMPGMSGTEVCQRLRSDPALAGLRIIAYTAHALPAEIQEIMAAGFDDILV
                     KPIDIRTLLAKVGIDPD"
     gene            complement(9447..>10164)
                     /locus_tag="VIO83_00225"
     CDS             complement(9447..>10164)
                     /locus_tag="VIO83_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8329750.1"
                     /translation="SQAKAESANRLKSEFLASMSHELRTPLNGIIGYAEYLQETAGDP
                     SSREFAGIIHKSSRHLLELVNDILDQASIEAGKMQLHLAEEDLSALVRDAIDMQWAFA
                     ESKGLTLAARLQADLPTTLRCDRTRLLQVLSNLLHNALKFTDAGHVSLLVSQADGYLC
                     LAVEDSGPGIALDQQEVIFERFRQIDTFVTRQHAGTGLGLALSRELVAIMGGSITLHS
                     APGRGSTFEVRLPLHPLENR"
BASE COUNT         1724 a         3364 c         3286 g         1790 t
ORIGIN      
        1 gctcgaagcc gtggcgcgcg aagccgccag caaggccaat ctgcaacctg aattcatcaa
       61 ggtgaccgac atgatggaga tcatggccta tgacctgctg tccacgccgg cgctggtgat
      121 caatgaaaaa gtggtcagca gtgggcgcat ccccacggtg gccgaagtgc agcagtggct
      181 gacggcttga gccagtaagc tggcgcagta accgtcacgg taggagcggg ggctctgggt
      241 agggcggcat tgctgcgctc agggcttcat gtcgttccgg ccctgatatt cggatggctg
      301 aatatgcgga cttgttttcg gcctgagcct cgccagttgt gcgcttcctt ggccttgcgg
      361 aaggtttgag caattgccct ccgatgcaga caggcgtcca taagcccccg caggaggcgg
      421 atttcgccgc tgcaggaacg ctcggtggcg gtcattcagc gacgctccga gatcttgcct
      481 tgggttctcg acacgccacc tcgctttact tgatatgcga caagcaatat attcgaaaaa
      541 tcatattcaa tggtgggccc atcatgggtc gcaatacccg ggtccagcga caaacctgac
      601 gctacgccat tcacgagatc cacgagcgct tcaagatgtt cgtgctagtg aagaacaaac
      661 attgaggact gccatggtcg agcacctgac ccccaccacg gttttcaaat gcctggctga
      721 cgagacccgg gcgcggatga ccctgctgat cgcccgcgag ggcgagctgt gcgtctgcga
      781 gctgaccgcg gcgctggagg aaagccagcc gaagatctcc cgccatctgg cactgctgcg
      841 cagctccggc gtgctggagg atcgccgcca gggccagtgg gtgtactacc gcctgcaccc
      901 gcacctgccg gactgggtgc acgacatgct gaagaccatc ctggccgcca acaagaactg
      961 gctgagcccc gaggtgcagc gcctggaagg catgcgcaac cgccccgagc gcgtcggcgc
     1021 ctgcggctga tcggtgagga aagcgatgaa ggttctgttc ctgtgcacgg ccaacagctg
     1081 ccgcagcatc ctctgcgaag cgctgttcaa ccatctggcc ccggccggcc tgcgggcctt
     1141 cagtgccggc agccagccga agggcttcgt gcatccgctc acgcttgaag ctttagcgag
     1201 agcaggagtg cctggcgaag gcctggccag caagcccagc gagacccatg ccgacctgca
     1261 gcccgatctg gttatcaccg tctgcgacaa ggcggccgga gaagcctgtc cggtgttttt
     1321 cggcccggcg ctcaaggcgc actggggatt ggcggatccc tccgaggttg aaggttcgga
     1381 tagcgaaatc gcggtggcct tcgatgccac agttgcacgg atcaccgccc gaactcaggc
     1441 ctttctcgcc ttgcccctcg atcagctgga tgccgatgcc ctgaaagccg agctggcccg
     1501 catcgccagc ctgtaacccg tttgccctgg atggacgcca tgtacgacga cctgcccaac
     1561 atcgatcccg acctgctcga cctgcctgac ctgggcaagc tcggcctacc ggcgcagccg
     1621 gaacacaagc cgcgcatcct gctgctctac ggttccaatc gcgagcgctc ctacagccgc
     1681 ctgctggtgc aggaggccgc ccgcctgctc gagcgcttcg gcgccgagac gcgcatcttc
     1741 gatccgtccg gtctgccgtt gccggacgat gccccggaaa gccatcccaa ggtccaggag
     1801 ctgcgcgagc tggtgctgtg gtcggaaggc caggtgtggt gctcgccgga gcgccacggc
     1861 gcgatgagcg cggtgttcaa ggcacagatc gactggatcc cgctgacgat gggggcgatc
     1921 cgtcccagcc agggcaagac cctggcgctg atgcaggtct gtggcggatc gcagtcgttc
     1981 aatgcggtca accagatgcg cgtgctcggc cgctggatgc gcatgttcac catccccaac
     2041 cagtcctcgg tggccaaggc cttcctcgaa ttcgacgagg agggccgcat gaagccatct
     2101 tcctactacg accgggtggt cgatgtgatg gaggaactgg tcaagttcac cctgctgctg
     2161 cgcggccgcg ccgactacct ggtcgaccgc tactccgaac gcaaggagtc agccgaagag
     2221 ctgtcccgcc gcgtcaacca gcgcgccatt tgatttgcaa tccgaaggaa tccgcatcgt
     2281 gcaacatccc tacgacatcc tcggcgtgcc cggctgctcc ggtcgcctga tcttcactcc
     2341 gtgccccggc agcaaggaca ccagcatcgc ctcggcgctg gacaccctca aggcggccgg
     2401 cgccgaggcg ctgatcaccc tgatgccggc cagcgagctg gagcagaacc aggctgccga
     2461 actgccggcg ctgtgcgccg cgcgcaacct ggaatggttc cacctgccgg tggccgacga
     2521 gcaagtgccg ctggacgact tcgccagcgc ctgggcgcag gcgcgcgaac gcatccgcgt
     2581 gctgcttgac gaggggcgca gcatcgccat ccactgcaag ggcggctcag ggcgcaccgg
     2641 gctgatcgcc gcgcgcatac tcatcgaccg cggcctgtcg cgcgccgagg ccgtcgccct
     2701 ggtgcaggca ctgcggccca aggccatcca gcatccggcg cacctcgcct ggatcgaaca
     2761 gttcgacgcc gcataaacct tcaccgggaa gagagaacaa gaccatgacc atcaaggtag
     2821 gcatcaacgg cttcggccgc atcggacgtc tggccctgcg cgccgcctgg gactggccgg
     2881 agctggagtt cgtgcagatc aacgacccgg ccgcggacgc ggcgacccac gcgcacctgc
     2941 tcaacttcga ctcggtgcac ggccgctggc agcatgaagc cggcagcgac ggcgattgca
     3001 tcgtcatcga cggcaagcgc atcaaggtca cccacaacag ggccatcgcc gacaccgact
     3061 ggtcgggctg cgacctggtg atcgaggcca gcggcaagat gaccaaggtc gcgctgctgc
     3121 aggcctacct cgaccagggc gtcaagcgcg tggtggtcag cgcgccggtg aaggaggagg
     3181 gcgcgctcaa catcgtcatg ggcgtcaacg accagctgtt cgatccggcc aagcaccgca
     3241 tcgtcaccgc ggcgtcctgc accaccaact gcctggcccc ggtggtcaag gtgatccacg
     3301 aggcgctggg catccgccat ggctcgatca ccaccatcca cgacctgacc aacacccaga
     3361 gcctgctcga ccagccgcac aaggatctgc gtcgcgcgcg tgcctccttg atgagcctga
     3421 tcccgacgac caccggctcg gccaccgcca tcgccgagat cttccccgag ctgcgcggca
     3481 agctgaacgg gcatgccgtg cgcgtgccgc tggccaacgc ctcgctgacc gactgcgtgt
     3541 tcgaggtgga gcgtgccacc accgccgagg aagtgaacgc cctgctcaag gccgccgccg
     3601 aaggtccgct caagggcatc ctcggctacg aggagcgccc gctggtgtcc atcgattacc
     3661 gcaccgaccc gcgctcgtcg atcatcgacg cgctgtcgac cctggtggtc aacggcaccc
     3721 aagtgaagat ctacgcctgg tacgacaacg agtggggcta cgccaaccgc accgtcgagc
     3781 tggcgcgtaa ggtcggtctg cctggctgaa aacgaccgcg ctcgactgcg tagggtgcgc
     3841 cctgcgcacc agggcgccgc gatcggtgcg cgcggcgcac cctacgtcga cttgcacggt
     3901 ccgaccgctg ggtgcgccgt gcgcatccag cgctccctcg cggcctgtcc acgcgggagc
     3961 gtaggcgcga tcaatgctcc agaacaccac cctgccgcac ggatttgcta tgcacgccct
     4021 gtcccgtctc tcccccgaaa tccgccagta cctcttggtc accggcaact actgggcctt
     4081 caccctcacc gacggcgccc tgcgcatgct ggtggtgctg catttccatg ggctcggcta
     4141 cagcccactg cagatcgccg cgctgttcct gttctacgag atcttcggcg tggtcaccaa
     4201 cctggtcggc ggctggctgg gtgcgcatct gggcctcaac cgcaccatga acatcggcct
     4261 gggcctgcag gtggcggcgc tgctgatgct caccgtgccg gcggccatgc tcaccgtgcc
     4321 ctgggtgatg gcggctcagg cgctgtccgg catcgccaag gacctcaaca agatgagcgc
     4381 caagagctcg atcaagttgc tggtggcgga tgggcagcag ggcaccctgt acaagtgggt
     4441 ggcgctgctc accggctcga agaacgcgct gaagggcgtc ggcttcttcc tcggcggtgc
     4501 gctgctgacc gggcttggct tcactggcgc ggtgctggcc atggccgcgg tgctggcgct
     4561 gatctggttg gccagtctgg tgaccctgaa gaaggatctg ggcaaggcca agagcaaacc
     4621 caagttccgc gacctgctgt cgaagagtcg ggcgatcaac atcctctccg ctgcgcgcct
     4681 gttcctgttc ggcgcccgcg acgtgtggtt cgtggtggcg ctgccggtgt acctggccag
     4741 caccttcggc tgggacttct ggaaggtcgg cggctttctc gccatctggg tgatcggcta
     4801 cggcatcgtg cagtcgttcg cgccgtactt caccggcaaa cgcagtggca agctgccgga
     4861 tggccgcgcg gccttcgtct gggctgcttt gctggccgga ctgccggcgg ccatcgccct
     4921 tggcttgggc actgaacttt ccgcgcagac ggtgctgatc ggcggcctgc tgctgtttgg
     4981 cgcgctattc gccatcaact cctcgctgca cagctacctg atcgtcagct atgccaagga
     5041 agacggggtg tcgctggatg tgggcttcta ctacatgtcc aacgccctgg gccggctgat
     5101 cggcaccctg ctgtcgggct gggtgtacca ggcctggggc ctcgaagcct gcctttgggt
     5161 gtccagcgcg ttcgtgctgc tcgccgcgct gatttccatc ggcctgccgc gacatgcccc
     5221 gatcgctcag gcatcctgaa cagtaagcag cgaaggaatt ggccaggcgc gcccatgccg
     5281 ggcgtgctgg cgcggaggca acacatggcg catgcacatc atcacgatca ccactctcac
     5341 gcacacgcgc cgcagcgcta cgaccgcgcc ttcgccattg gcatcgtgct gaatctcggc
     5401 ttcgtgctgg tcgaggcctt ctatggctgg caggtgaact cgctggccct gctggccgac
     5461 gccggccaca acctcagcga tgtcggtggg ctgatcctag cctgggcggc cctggcggct
     5521 acccgcctgc gccccaatga ccgccacacc tacggctggc agcgcggctc gatcctcgcc
     5581 agcttcacca atgcctcgat cctgctggtg gccatgggct cgctgggctg ggagtcgatc
     5641 cagcgcctgc aacagccggt ggcgatcgtc ggcgagacgg tgatggtggt cgccggcatc
     5701 ggcgtgctgg tcaacgccgc caccgccgcg ctgttccttt ccggcagccg cagcgacctc
     5761 aacctgcgtg gcgccttcct gcacatggcg gccgacgccc tggtgtcgat tggtgtggtg
     5821 ctggccgggg ggctgtatct gtggcagggc tggagctgga tcgatccgct ggtcagtctg
     5881 gcgatcgctg tggtgatcgt gctcggcacc tggtcgctgt tccgccagtc gctgcacctg
     5941 ctgttcgacg gcgtgccgga gcacgtcgac ctgccggcgg tgcgccagta cctgctggag
     6001 cagccgggcg tctgcgacgt gcacgatctg cacgtgtggg cgatgagtac cgccgaggtg
     6061 gcgctgaccg cgcacatggc catgaatccg ctgcccgagg atgaccgctt cctgcagggc
     6121 atcgcccacg agttgcacga gcacttcggc atcagccatg ccacgctgca actggagagg
     6181 gatctcggct gtccgctggg ctgcgcccgt cttccctgcc gttaagctgc ggcgtcactc
     6241 tgggcagtag gcccaggcgt tcggggcgtg agggtattgc gcgccggtat cggggtgccg
     6301 ccagagatct tcatcgtacc ccagcagcac gatgaagcca tgtgggcgga agtcggcgag
     6361 cacctcggtc ttctcggcgg ggcgggtttt cgccgtttcc acacggttgc tgtccatgta
     6421 ggtgatcagt gcacgtgcgc cgccgagacg attgacgatc tgcagctttc gaacgtcgcg
     6481 agacattgga ggccaacctc gttgctgtgt gttgttaccc ctattttccg tgtggatgat
     6541 ttcaatctgg cgaacagcag atgaccagtg tcttgctcgt gtttcactgc ggacctcctg
     6601 gtggcctcga agctcgagcg cctggggcga gacccgcgac aacctcgtga actcaggatt
     6661 tgctcgccga atgagcagcc ggctgtacac agcctgacct tgtccggggc cagcggttaa
     6721 cgatggtctt ggctgaacgc tttttcgtca cagactacgg cggtgcgctc ggttagaatc
     6781 ccgccatttt gtcctgcccg cgaagattgc cagtgtccat tgcccgccgt ttcgccttgc
     6841 ctaccttgtt gccctgcctg ctcgccagcc cggccgggca ggccgccgac ctgtttcttg
     6901 acggcgaaca gctgcccgag gtgctcactg ctacccggct caagcaatcc cctgcggcgg
     6961 tgccaggcag cgtcacggtg ctcgatcgtg cgctgatcca ggccaccggc gcgcgcgaca
     7021 ttcccgagct gatgcgcctg gtgccgggga tgatggtcgg ctacaccaag ggcaacctga
     7081 gcacggtcaa ctatcacggc ggccgcgtca gcgaggcccg ccgcctgcag gtgctggtcg
     7141 acggccgctc ggtgtaccgg cctggcctgg ccaccgtgga ctggacggac ctgcccctgg
     7201 ccatcgaaga catcgagcgt atcgaggtgt ttcgcgggcc gaacaccgtc agttatggcg
     7261 ccaatgccct ggccggggtc atcagcatca ccacccgccg tccagcagat agccagggca
     7321 cccgcctgaa gtacacccgc ggcgagcgcg gcgttgacga ctggtacgcc agcgaaggct
     7381 tccacgccgg cagcggcgat tttcgcttgt cgctctccgg gcaggaggac aatggcttcc
     7441 accataccga tgagggcgaa aaataccgcg atagtcggcg cagcacgcgc ttccagctga
     7501 gcgccagcca tgtcctgaac gacgagcaga gcctggactg gcaagtggcg gccaaggaag
     7561 gcagcaacca gcgcaactat tactccgatg aagaggcgct gatcagaaac gtggtcccgc
     7621 gcggtgacac cgacaaggat gtggtagccc gcgattatgc cggctccgtg cgctggagcc
     7681 tcgacgccag cgccgaccac aacgtgcagg tgcaggccag tgcccagcgc tgggaacgcc
     7741 gccaggagtg gcgggcctgc gacctcgccc tgtccttcac ccccgaggtg agccagctgt
     7801 tcgccctgaa cccggacagc gtggatacgc tggcacgcct gctcagaaaa ggccgaggcg
     7861 atgccgttga gaccttgctc aaggggcagg agcgggctct ggccagagtc atccgcgaca
     7921 actacaccgg gccagacggc gccgcactcc gcgctcccgt gtgcggcgat atcaacaaca
     7981 acatcgacga aacccgcttc gacctggagg tccaggatac gctgagcctc acggacaacc
     8041 tgcgtctgct cagcggcgcc agctaccgtc gtgacattag cagctcggaa acctacttcg
     8101 acggccgcct cagcaacgat atctggcggc tgttcggtca tctggagtgg tacctcaacg
     8161 agcacatcgt cctgcagggc ggcgcgatgt acgaacagga caagttcagt ggcgactcac
     8221 tgacgccgcg cgtggccctc aactatctga tcaccccgcg ccacggcctg cgtttcgtct
     8281 actcggaggc ggtgcgctca ccggacatgt tcgaggaccg ggtgcagtgg cgttaccgca
     8341 tggaaaacct ctccggagac gccgcgggcc gcgactcggc ctactactac gcctacgcca
     8401 acgggccgaa gaatctcgac caggaaatca tgcgttcgcg ggagatcggc tacaacggtc
     8461 agttcgactt cggtctgcgc atcgacatca gggtgttcca cgaagaaatc caccagatga
     8521 tcagtgaacc actggacatc accgaattcc ggccgagcaa cgacaaccag atgtggttcc
     8581 gcggcgcgga aaccgagatc gactggcaaa tcgacagtgc cgaccgcctg cgtctgacct
     8641 atgcctacgt cgatttcgcc gccacggcca agattgatcg ccgcctgact gcccgctaca
     8701 gcggttccgg cggctggatg cgtgactggg gacgcggctg gtcgagcagc ctgttctact
     8761 acggcgccga cctgctcaac gaacgccgtt tcgaacggct cgacctgcgg ctggccaaac
     8821 gtctccgcct gggcgcgacc gaactcgaac tggccggcgt gctgcaacag cggctggacg
     8881 acgaacccct gacctggcgg gaaaacctct acgacgaccg ccggcagctg tacttcagcg
     8941 cgcagctcga gttctagact ccggtgcttg ttccgcgcgg cttcacctgc gccagccgag
     9001 cgggggaggc gagcaaagcc gctgctccgc ttgccggctg tcaccccacg ggagcgccag
     9061 ccgatcccct tggcccgtca gcggcgtcaa tcagtccgga tcgatgccca ccttggccag
     9121 caaggtccgg atgtcgatcg gcttgaccag gatgtcgtcg aagccggcgg ccatgatttc
     9181 ctggatttcc gccggcaggg cgtgggcggt gtaggcaatg atgcgcagcc cggcgagggc
     9241 cgggtcgctg cgcaggcgct ggcatacctc ggtgccggac atgcccggca tcatgatgtc
     9301 gagcaggaca cagtcgaatt gctcgctgct ggccatccgc agggcatccg tgccgttgtc
     9361 gcactcgacc acgctgcagc catagcgctg cagcaggacc cggggcaggg tgcggttgaa
     9421 ggcggtgtcg tcgaccagca ggactttcat cggttctcca ggggatgcag cggcaggcgt
     9481 acctcgaagg tactgccccg gcccggtgcg gaatgcaggg tgatgctgcc gcccatgatg
     9541 gccaccagct cgcgtgagag ggccagcccg aggccggtgc cggcgtgctg gcgggtaacg
     9601 aaggtgtcga tctggcggaa gcgctcgaag atcacttcct gctggtccag ggcgatcccg
     9661 gggccggagt cctcgaccgc gaggcacagg tagccatcgg cctgcgacac cagcagggac
     9721 acatgaccgg cgtcggtaaa cttgagtgca ttgtgcagca ggttgctgag tacctgcagc
     9781 aggcgcgtgc ggtcgcagcg cagggtggtc ggcaggtcgg cttgcagtcg ggccgccagg
     9841 gtcaggccct tgctctcggc gaaggcccac tgcatgtcga tggcatcccg gaccagggcg
     9901 gacaggtctt cctcggcgag gtgcagctgc atcttgccgg cttcgatgct ggcctggtcg
     9961 aggatgtcgt tgaccagttc cagcaggtgc cgactgctct tgtggatgat gccggcgaac
    10021 tcgcggctgc tggggtcgcc ggcggtttcc tgcaggtatt cggcgtagcc gatgatgccg
    10081 ttgagcggcg tgcgcagttc gtgcgacatc gaggccagga attcggactt gaggcggttg
    10141 gccgactcgg ccttggcctg gctg
//
LOCUS       DATCUN010000009        12820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_150262, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000009 DATCUN010000000
VERSION     DATCUN010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 12820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12820
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150262"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VIO83_00230"
     CDS             complement(<1..458)
                     /locus_tag="VIO83_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03761 family integrating conjugative element
                     protein"
                     /protein_id="HEY8329751.1"
                     /translation="MATTNEPLQLNLGSLRSAMSLTLHTHHASRIWHGRAAAEGRPGI
                     VGLNGYIAQMNKMRRGSEQDDPYSDFWMLRIEDKLEQTKATLQSLREQVDQALASVPP
                     ALTLGENLNVQPVKLPLFVNAQLGFAAVYLLADYDDIARKLILAHHTALI"
     gene            complement(907..2160)
                     /locus_tag="VIO83_00235"
     CDS             complement(907..2160)
                     /locus_tag="VIO83_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003158609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HEY8329752.1"
                     /translation="MAVDDTAPRHGPVALADLFDAALKDLAPTPTPTRMPIPAPSPAT
                     PAASGDAFLFSGNRHETVPRRLFLDRRLTPLERNAWQVFRLMQGDDGVTAFPTYEQLR
                     PWLASMPCAGLASHETVARALTLLRLTRWLSLVRRRRDAKTGRILGNLYVLHDEPLTP
                     FEAMQLDPDYLQLVSQALGHSAKAVQIVGLHTLKEIGEDPMLAGRTLPSRLQVLAERL
                     ANQGITATESYPQDDATHNSEEGESSLLRNPDDPTSDSEAGPKPAPDGSLRNPKQART
                     VRSSRMNEVRTTAQAQAQARALGDLQWPKRFAELKAEQQAGARVALQQVDAALRQAVL
                     DEWATRCSSHGIRNPAGYLFGIIQRAIHGEFNAWAKKDAPSAHAPPNERLPAAPPPSQ
                     PQGKPVPPEVARQHIERLRNLLASK"
     gene            complement(2164..2724)
                     /locus_tag="VIO83_00240"
     CDS             complement(2164..2724)
                     /locus_tag="VIO83_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017513942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HEY8329753.1"
                     /translation="MSASHPLNQAVIAQALYDLRNGQLRRCKAMGFSEAELDALKHPA
                     MVSVLANANVSWCSVSVNREVLRRLLSQAQDVEKEIATVDRMLRLGASTEMVSRFYGL
                     THQEVALRREVLGLPKRKGRHPVLDEKQDVELWRRWKAITSSRNVDLEDETSILDAAM
                     DLAGGMDLPLSVVWAAIKSWVDQGLG"
     gene            complement(2740..4374)
                     /locus_tag="VIO83_00245"
     CDS             complement(2740..4374)
                     /locus_tag="VIO83_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HEY8329754.1"
                     /translation="MAEMTSQQMAGKLLAAGFERSGPSTPALSDPIADTPMVVTLDQL
                     RPYDHDPRKKRNPAYEDIKASIRERGLDAAPAITRRPGEDHYIIRNGGNTRLAILREL
                     WSETKDEQFFRISCLFRPWPSRGEVVMLTGHLAENELRGGLTFIERALGVEKAREFYE
                     QENGTALSQSELARRLAADGFPVQQSHISRMNDAVRYLLPAIPTVLYGGLGRHQVERL
                     SVMRKACMLAWERYAKGRTLVQDFDDFFQEVLSQFDTQADEFTPQRVQDELIGQMSEL
                     LGIDYDVLALDLTESESRHRALVSDPTPPSAPPALPEPGALARPSVAPTPSAPTPAAT
                     PGARDREGGSKTDDSPAGSPVAAPGDLLQGHIVSPAPTTERLQSIQRMVADELGDALP
                     DFSANVLQSIPVQAGGLYPISDVWHIDAGLDTPDRLRIHIAQFAREITGEAGLDQCIE
                     DRADGVGFACHASAQPPSPQGRAVLALLASLSGQSFTDADLDGGQLAAELAALLHGHG
                     DAPRRLSDTALVKLFRLLRLARRLLDLESGTAGPGA"
     gene            complement(4367..4615)
                     /locus_tag="VIO83_00250"
     CDS             complement(4367..4615)
                     /locus_tag="VIO83_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEY8329755.1"
                     /translation="MANAHELARGHKRLRALIEFAVGEGWHVKRTPGGHLKFTKAGCA
                     AIYTSSTASDHRAALNARAQIRRAEREAQSPAQGGRHG"
     gene            complement(4599..5474)
                     /locus_tag="VIO83_00255"
     CDS             complement(4599..5474)
                     /locus_tag="VIO83_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY8329756.1"
                     /translation="MQVVSIISTKGGVGKTTTAANLGGLAADAGLRVLLLDLDVQPTL
                     SSYYELGHRAPGGIYELLAFNERDLGQLVSRTIITGLDLVLSNDHRGELNTLLLHAPD
                     GRLRLRHLLPVLAPLYDLVLIDTQGARSVTLEMAVLASDLALSPVTPEILAARELRRG
                     TMQLLQDIAPYRHLGIEPPPLHLLINRVHPVSANARLIQQALRDLFQDHAGIRVLATD
                     VPAIEAYPRAATRGLPVHRVEYRQPPGRVAAAALDTMRGLAGELFPQWQHRFATVSGR
                     PPFPLDTGRSHGERT"
     gene            complement(5517..5729)
                     /locus_tag="VIO83_00260"
     CDS             complement(5517..5729)
                     /locus_tag="VIO83_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HEY8329757.1"
                     /translation="MSQAPVLPPNERRILRLEEVEAKSGFKRAHLYNLMRAGKFPKAL
                     RLGVRAVGWDSIEIDEWIAERVNSRA"
     gene            complement(5849..6595)
                     /locus_tag="VIO83_00265"
     CDS             complement(5849..6595)
                     /locus_tag="VIO83_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329758.1"
                     /translation="MNNNAPTSKVFYRPIEASIRWTGLLRFEQIILASISSPRNLPLS
                     LDCPRWGELRLYTERIYDGILNGELPFGQNGITTRDTALIDAPDLTVRHVDLKRWMQQ
                     HYPEQRPGFLFSRSERIAHPFISLETGQAMLIERLALKSALEQCKRQLHELQDKHDAL
                     LKQSTVIPACAQCPISDRAEATYLHIIGTMLELMLGQSPSGTPYSSFNSQEAIATAMI
                     AHHGELMGITDRTLQAKFAQARRKLRSAVS"
     gene            7440..7513
                     /locus_tag="VIO83_00270"
     tRNA            7440..7513
                     /locus_tag="VIO83_00270"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7472..7474,aa:Cys,seq:gca)
     gene            complement(7957..8661)
                     /locus_tag="VIO83_00275"
     CDS             complement(7957..8661)
                     /locus_tag="VIO83_00275"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8329759.1"
                     /translation="MLVEGSSHAARRGKAPRAGHAYQHLKARILEGDLGPGDRLSVLA
                     LAQALACSRVPVMEALKRLQAEGLIDIVPQVGCQVAAPTAGNVEDFFAAFAAVEGTVA
                     QLAAQRRSEADLIAFGQTLARIDQALQNAGGPQDRDPVYRRLNRDFHSAIHAMACAPA
                     ASEIAAALWDRSDFLIKVAFGSLYFSPCVRAAHAAVCAAITTGDAAAAQAAMTQHLEQ
                     VGAAVAARLREMDSGR"
     gene            8742..10028
                     /locus_tag="VIO83_00280"
     CDS             8742..10028
                     /locus_tag="VIO83_00280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8329760.1"
                     /translation="MTDKIRLGAFLLPSAGATIAQYEQGFAGQNPNFYQNTLRDSARL
                     IRQLDDIGFDFVAFSEHHFHLEGLELSNNPILLGAWAAGLTRKLRIGQMGNVLPARNP
                     LLMAEDLAMLDHFSEGRMMAGFARGYQARHVATIGQKFNAWSTAANDPKYQEHDRTNR
                     ELFAEHYDIIRRAWREPLFRHQGQHWQLPPPGIPWNHPATLDMAPGMVGADGMLEKVG
                     IAPMTLQDPATIESYIPFTMSTATLQWAARNEVIPILFTPIDELVRGCIDAFQGAAQE
                     AGRQLEWGRGLGHFREILIADTDQEAVALMENGLGYVWPRWHHWFGFNEALRNPGEQG
                     EIANTAATVRERGYSLCGSVDTVTRLLERMLKTLNTNLIVPWISVGPMPIDALLKSND
                     LLVEKVLPRLGIELERFQPTLRQEFTGDLWRDASQR"
     gene            10049..11500
                     /locus_tag="VIO83_00285"
     CDS             10049..11500
                     /locus_tag="VIO83_00285"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8329761.1"
                     /translation="MSIRTGAQFLAGLKDDREVWYDGERVRDVASFEPFAASVKSLAT
                     LYDMQHDPQHRDILSVDCPEFGERIARPFQIPRTPEQLRLKREAYRIWAEATCGMMGR
                     SPDFLNVMMASLAAKRSFFAEFSEERADAVVAYYRHVASNDLFLTHALLDPQLDKGKL
                     RHQQTDPTITLHVKERTKDGLLVHGAKRIATAAPYADELLVWPFPPTFQPGEEPYANV
                     FAVPMNTPGLKTICRPSFAHPGRRKDYPLSAQFDEMDATVVFDNVLVPWERVFLHEDI
                     GLLNRMYRDTRMRELTAHQTNVRLETKLGFVYAVLLRVAQASGIDTRKDVIEMLGEAV
                     MNLEAIRNTTRSAELQATLDPENGVLYPDLFALQCGRALGPVVYPRAVQMIRKVAGGG
                     LVQLPNSMHEFDSPIGADLERALRGAGIGGRDKAQLLRLAWDICGSEFGARHELYEMN
                     YAGEYGALLAGIQREYGRKSYYLDHLDRFMSTL"
     gene            11497..12363
                     /locus_tag="VIO83_00290"
     CDS             11497..12363
                     /locus_tag="VIO83_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8329762.1"
                     /translation="MSTHHSFWADLRAGSFSQGYLAAGGIRTRYLHAGERGMPPLILI
                     HGTGGHAEAYTRNLIAHGEHFDTYAIDLVGHGWSDKPLAPYEISVYVRHLRAVLDTLG
                     FDRVHLSGESLGGWVGARFALEYPDRVDRLVLNTTGGATLDLGVMERIKTLSTAAVEN
                     PSWETIKARLEWLMADPAVVTDDLVACRQAIYAQPAMRAAMPHILCLQEPDIRRRNNL
                     TDADWAAIKAPTLVLWTDKDPTAAVSVGQRIADLIPNARLEVMERCGHWPQFEDAPRF
                     NDLHIGFLGGRG"
     gene            12370..>12820
                     /gene="mhpB"
                     /locus_tag="VIO83_00295"
     CDS             12370..>12820
                     /gene="mhpB"
                     /locus_tag="VIO83_00295"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="catalyzes the cleavage of 3-(2,3-dihydroxyphenyl)
                     propionate into 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate;
                     part of the 3-phenylpropionic acid degradation pathway;
                     member of the protocatechuate 4,5-dioxygenase family;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HEY8329763.1"
                     /translation="MPLAALFASHTPLKDYLDPGPGIAAEVDACLAEARAWVQAFAPD
                     LVIEFAPDHFNGFFYQLMPSFCIGAAAESVGDWKTATGPLPVAGELAQRLVEAVHADG
                     VDVALSQRMTVDHGFTQLLEILFDAGKAPPLIPVFINCAAPPRPPMAR"
BASE COUNT         2117 a         4111 c         4374 g         2218 t
ORIGIN      
        1 tcgatgagtg cggtgtggtg cgcgaggatc agcttgcggg cgatgtcgtc gtagtcggcc
       61 agcagataca ccgcggcaaa gcccaactgc gcattgacga acagcggcag cttgacgggc
      121 tgcacgttga ggttctcgcc cagggtgagt gccggcggca cgctcgccag ggcctggtcc
      181 acctgctcgc gcagcgattg cagcgtggcc ttcgtctgtt cgagcttgtc ctcgatgcgc
      241 agcatccaga agtcgctgta cgggtcgtcc tgctccgagc cgcgccgcat cttgttcatc
      301 tgggcgatgt agccgttcag gccgacgatc cccggtcgtc cctcggcggc ggcgcggccg
      361 tgccaaatgc gcgaggcgtg gtgcgtgtgc agcgtcagcg acatcgcgct gcgcaaggag
      421 ccgagattca gttgcagggg ttcgttggtc gttgccatgg tgtccgctcg ttgttggaaa
      481 agagcggaca gcttcggcag gtggcggaag gcagtcagtc aacaaaccga aacccgccag
      541 tccccggttc acggcgcagc gggtgcatgc ggcgcgagct atcccctggg gatagcgcca
      601 tggagcgcca aggaacgctg cacgcctgga tcagcgtgga cagggccgat cccacggcag
      661 gtgatcgggg cttggcgtgc agcggttcga cgtcgtagct gtcagtggaa ctatcccctg
      721 gggatagttc catggagcgc caaggaacgc tgcgcgcccg ggtcaagcgc ggacaaggcc
      781 gatcccgccg caggcgatcg gggcttggcg tgcagcggtt cgacgtgtag ccgtcagtgg
      841 aactatcccc tggggatagc tctactggaa gccactggca tccacttcac cgccttgcag
      901 gcccgctcac ttgctggcga gcaggttgcg cagccgctcg atgtgctgcc tggcgacttc
      961 tggcggcacc ggcttgccct gcggctggga tggtggcggt gctgctggta gccgctcgtt
     1021 cggcggagcg tgtgccgatg gcgcgtcttt cttggcccag gcgttgaact cgccatggat
     1081 ggcccgctgg atgatgccga acagataccc cgcaggattg cggatgccgt ggctgctgca
     1141 tcgcgtggcc cattcgtcca gcacggcctg cctcagcgcg gcatcgacct gctgcaatgc
     1201 cacccgcgca cctgcctgct gctctgcctt cagttccgcg aagcgtttgg gccattgcag
     1261 gtcgcccagc gcgcgcgcct gtgcctgcgc ctgcgcggta gtacgtactt cattcatacg
     1321 actactacgt actgtacggg cctgcttcgg attccgaaga gagccgtctg gcgcgggttt
     1381 cgggcctgct tcggaatccg aagtggggtc atcaggattc cgaagaaggc tcgattcccc
     1441 ttcttcggaa ttgtgagtgg catcatcttg tggataactt tcggtcgccg tgatgccctg
     1501 gttggcgagg cgttcggcca ggacttgcag ccttgacggc agggtgcgcc cggccagcat
     1561 cgggtcttcg ccgatttcct tgagggtgtg caggcccacg atctgcacgg ctttggccga
     1621 gtggccgagc gcctggctga cgagctgcag gtagtccggg tcgagctgca tcgcttcaaa
     1681 gggcgtcagc ggctcgtcgt gcaggacata gaggttgccg aggatgcggc cggtcttggc
     1741 gtcgcgccgt cgccgcacca ggctcagcca acgggtcagg cgcagcagcg tcagcgcccg
     1801 cgccacggtt tcgtgcgatg ccagcccagc gcagggcatc gaggccagcc agggccgaag
     1861 ctgctcatac gtcgggaagg cggtcacgcc atcgtcgccc tgcatcagcc ggaatacctg
     1921 ccaggcgttg cgctccagcg gcgtcaggcg acggtcgagg aacaaccgcc gcggcacggt
     1981 ctcatgccgg ttgccgctga agaggaacgc atcgccggac gcggccggtg tcgcaggtga
     2041 tggcgcaggt ataggcatgc gcgtgggggt gggcgtgggc gcaaggtctt tcagcgccgc
     2101 atcgaacagg tcagcgagtg cgacggggcc atggcgtggt gcagtgtcgt ccacggccat
     2161 ggctcaaccc aatccctgat cgacccacga cttgatcgcg gcccagacca ccgacagcgg
     2221 caaatccatg cctccggcga ggtccatggc cgcgtcgaga atcgaggtct cgtcctccag
     2281 gtccacattc ctgctgctgg tgatggcttt ccagcgccgc cacagttcga cgtcctgttt
     2341 ctcgtccagg acagggtgac gccccttgcg cttgggcagg cccagcacct cacggcgcag
     2401 ggcgacttcc tgatgggtca ggccatagaa gcggctgacc atttccgtgc tggcccccag
     2461 gcgcaacatg cgatcgacgg tcgcgatttc cttctccacg tcctgcgcct gcgagagcag
     2521 acgccggagc acctcgcggt tcacggacac ggaacaccac gagacattgg cgttggccag
     2581 cacgctcacc atggcgggat gcttgagcgc atccagctct gcctcactga aacccatcgc
     2641 cttgcagcgg cgcagttggc cgttgcgcag gtcgtagagc gcctgggcga tgacggcttg
     2701 gttgagcggg tgcgaggcag acatgctggt ctccttcctt cacgccccag ggcctgcggt
     2761 gccggattcc aggtccagca ggcgccgggc caggcgcagc aatcggaaca gtttgaccag
     2821 cgcggtgtcg ctcaagcgcc ggggcgcatc gccatggccg tgcagcagcg ccgccagctc
     2881 ggcggcaagc tgcccgccgt ccagatcggc atcggtgaag ctctggcctg acaggctcgc
     2941 cagcaatgcc agcacggcgc ggccctgcgg tgatgggggc tgcgcgctgg catggcatgc
     3001 gaagccaaca ccgtccgcgc ggtcctcgat gcactgatcg aggccggcct cgcccgtgat
     3061 ctcacgtgcg aactgcgcga tgtggatgcg caggcgatcg ggcgtgtcca ggcccgcgtc
     3121 gatgtgccag acgtcggaaa tcgggtagag accgcccgcc tggacgggaa tggactgcaa
     3181 gacattggcc gagaaatcgg gcagcgcgtc ccccaactcg tcggcgacca tccgctgaat
     3241 ggattgcagc cgctccgtcg tcggtgcagg cgaaacgatg tgcccctgca gcaaatcacc
     3301 aggagcagcc acggggcttc ctgcgggtga gtcgtccgtc ttcgagccgc cctcgcggtc
     3361 gcgcgcccca ggcgtggccg ccggcgtcgg ggccgatggg gttggtgcga cggacgggcg
     3421 tgccaaggct ccaggctcag gcagcgcggg cggtgccgat ggtggggtcg gatcgctgac
     3481 cagagcgcga tggcggcttt cggattcggt gaggtcaagc gccaatacgt cgtagtcgat
     3541 gccgagcagt tcggacatct ggccgatcag ctcatcctgc acacgctggg gggtgaactc
     3601 gtcagcctgc gtgtcgaact gcgacagcac ctcctgaaag aagtcgtcga agtcctgcac
     3661 cagcgtgcgg cccttggcgt agcgctccca cgcgagcatg caggccttgc gcatgaccga
     3721 caggcgttcg acctggtggc ggccgagccc gccgtagagc acggtcggga tcgccggcag
     3781 gaggtagcgc accgcgtcgt tcattcggct gatatgggac tgctggaccg ggaagccatc
     3841 ggcggcgagg cggcgggcca gctcggactg gctcagggcg gtgccatttt cctgctcgta
     3901 gaactcacgc gccttctcga cgccgagggc gcgctcgatg aacgtgagcc ccccgcgcaa
     3961 ttcgttctcg gccaggtggc cggtcagcat cacgacttcg ccgcggctgg gccacggccg
     4021 gaataggcac gatatgcgga agaactgctc gtccttggtc tccgaccaca gttcgcgcag
     4081 gatcgccaga cgtgtgttgc cgccgttgcg gatgatgtag tggtcctcgc ccgggcgccg
     4141 ggtgatcgct ggcgccgcgt ccaggccgcg ctcgcggatg gatgccttga tgtcctcgta
     4201 ggccggattg cgcttcttcc tggggtcgtg gtcgtagggg cgcaactggt ccagggtcac
     4261 gaccatgggc gtgtcggcga tcgggtcgct caaggccggg gtggacgggc cgctgcgctc
     4321 gaacccggcc gcgagcagct tgccggccat ctgctgggag gtcatctcag ccatggcggc
     4381 ctccctgcgc tggagactgg gcctcgcgct cggcgcggcg aatctgcgcg cgggcgttga
     4441 gcgccgcccg gtggtcgctg gccgtcgaac tggtgtagat ggcggcgcag ccggccttgg
     4501 tgaatttgag gtggccgcct ggcgtgcgct tgacgtgcca gccctcgccg acggcgaact
     4561 cgatcagggc gcgcagccgc ttgtggcctc gggccagttc atgtgcgttc gccatgggac
     4621 ctcccggtat caagagggaa tggcgggcgg ccggacaccg tggcaaatcg gtgctgccat
     4681 tgcgggaaca gttcgccagc gaggccgcgc atcgtgtcga gcgcggcggc ggcgactctg
     4741 cccggtggct ggcggtactc gacccgatgc accggcaggc cgcgcgtcgc ggcgcgcgga
     4801 taggcttcga tggccggcac gtcggtggcc agcacgcgga tgccggcgtg gtcctggaac
     4861 aggtcgcgca gggcctgctg gatcagccgg gcgttcgcgg acaccggatg gacgcggttg
     4921 atgagcaggt gcagtggcgg cggttcgatg cccaggtgtc ggtacggcgc gatgtcctgc
     4981 agcaactgca tggtgccgcg ccgcagctcg cgggcggcga ggatttccgg ggtcacgggc
     5041 gacagcgcga gatcggaggc gagcaccgcc atctccagcg tcaccgagcg cgcgccctgg
     5101 gtatcgatca gcaccaggtc gtagagaggt gcaagcacgg gcagcaggtg ccgtagccgc
     5161 aggcgtccgt ccggcgcgtg cagcagcaag gtgttcagct cgccccggtg atcgttggag
     5221 agcaccaggt ccaggcctgt gatgatcgtg cgggacacga gctggccgag gtcgcgctcg
     5281 ttgaaggcca gcaattcata gatgccgcca ggcgcgcgat ggcccagctc gtagtaggac
     5341 gacaaggtgg gctgcacgtc gaggtcgagc agcagcacgc gcagccccgc atccgccgcg
     5401 agtccgccca ggttggcggc cgtggtggtc ttgcccacgc cgcccttcgt tgaaatgatg
     5461 gatacgacct gcatggcgtt ctccgtgtga ggatggaaag tccgcgggag aacgggtcag
     5521 gcccggctgt tgacgcgctc ggcgatccac tcatcgatct cgatggaatc ccagccaacg
     5581 gcgcgcacgc ccaggcgcag cgccttgggg aacttgccgg cgcgcatcag gttgtagagg
     5641 tgggcgcgct tgaagccgga cttcgcttcg acttcttcca ggcgcaggat gcggcgctcg
     5701 ttgggcggca gtacaggtgc ttgcgacatg gcggtcactc ctgaacgctc agtggcgttt
     5761 gttggcgtga cacctattca aatgtcggct tctgtcccag accaccgcaa atgcggtttc
     5821 cgcgaccgca gatacgggct tgcaaatatc aggagacggc ggatcgcagc ttgcggcggg
     5881 cctgtgcaaa cttggcttgc agtgtgcgat cggtaatgcc catgagttcg ccgtgatgcg
     5941 cgatcatcgc ggtagcgatg gcttcctggc tgttgaagct ggagtacggt gtgcccgatg
     6001 gggattggcc gagcatcagt tccagcatcg tgccgatgat gtgcaggtag gtggcctcgg
     6061 cccgatcgct gattgggcac tgcgcgcatg caggaatcac cgtggactgc ttgagcagcg
     6121 cgtcgtgctt gtcttgcagt tcgtgcagtt ggcgcttgca ctgttccaag gcagatttaa
     6181 gagcgagccg ttcaatcagc atggcttggc cggtctccaa cgagatgaat ggatgggcga
     6241 tgcgctcgct gcgggagaag agaaagccgg gtcgctgctc gggatagtgc tgctgcatcc
     6301 agcgcttcag atcgacgtgg cgcaccgtca gatcaggggc gtcgatcagt gcggtatcgc
     6361 gtgtcgtgat gccgttctgc ccgaagggca gttctccatt gaggatgccg tcgtagatgc
     6421 gttcggtgta cagcctcagt tcgccccaac gcgggcagtc caacgacagt ggcaggttcc
     6481 ttggcgacga aatcgaggcc aggatgattt gctcgaatcg cagcagcccg gtccagcgta
     6541 tggaggcttc gatcgggcgg tagaacacct ttgatgttgg tgcattgttg ttcatgctcc
     6601 catctccttc caggagaact acatggccga ctcctttggc gccagccgga aggttggcgt
     6661 gttgtctgtg catgaagcgg gtcccgaggt ccgcttcgtc atggggcctg agtacttctc
     6721 accggcttgt gttgcaggta gccgtcgtgt gctcgatgtg caagaattca gtgagtcgca
     6781 agatggtcgg gcctgccgct ggcagactcg aaagcgacgc gcacagtagc agcgtcataa
     6841 gtagcggcga tcttcgcaaa accgtttggg tatggcctga tatgccagcg gtttcacgag
     6901 gcgcgaaggg ctcgcaatcg ctgcccgacg tccacaagga cgcggtgaac ccccagcctg
     6961 tcatgcttgc ccagcatcct gatatagcga caaagaatcg cgcgctgacg cgccacgact
     7021 agggtgcgcg ttcttcctgc atgatttgcg cggacgagct acgcaatgcg cggtgcatgc
     7081 ggctcgtagc tggcatccga actgagccag caccgagagg gtcggcctgg acgtcatcgc
     7141 ggtagcggtg cgggcaccga gagcaggggc acaggcggcc agttcgagac cgtcgctgac
     7201 gacgttgtga cgagtgtggg tccggcgtgc tgagtgcggg tctatggacg ccagtggaac
     7261 tctttggacg atcttgttgg cgggggccaa aaactaggct acaatctagg tcttcgctgg
     7321 tcacctcggc gaagaaagtg ttcgggaaac aacgacttac gcgcctagta aatgtctgat
     7381 ttcccgctcc atgtcttttc ctggccgcgc cccgtgcgcg gccagtcgtt tccggccccg
     7441 gctaggtggc agagtggtca tgcagcggcc tgcaaagccg cgtacgtcgg ttcgattccg
     7501 gccctagcct ccacgcctcc gttcgcatag cgccgcatag cgccgaaaac ccgcgtaaaa
     7561 ccgagctttc cggcctatgc gtaggccgct actgtccgct cgcgtccatt gatagccgat
     7621 atttcgtcgg tactttcgac ggtaccgacg ccctgggccg ggggcgaaac cgtcacaggt
     7681 atttaccgga cctgacgggg gattgagccg atagccacgc tgggcgagct tcgagcgcaa
     7741 ctcgcgcgcc gaaccggcat tgacgtccat gagcaaaccc ttgtctcgac cttctgcctg
     7801 aaggtcgggt agatcgacgc gtgagcgtcc cttgatttca ccggcggtca cccggaaagc
     7861 actgagaatt ctgcgcgcct cgaaaagcag cttgccgtgc aaatgacagg atttgtacaa
     7921 tctgtgggcg gcttgcgcct gctgcagggc aaagcgtcat cgccccgagt ccatctcgcg
     7981 cagccgcgcc gccaccgcgg caccgacctg ctccagatgc tgcgtcatcg ccgcctgggc
     8041 ggcggcagcg tcgccggtag tgatggccgc gcacacggcg gcgtgcgcgg cacggacgca
     8101 cggcgagaag tagagggagc cgaacgccac cttgatcagg aaatcgctgc gatcccaaag
     8161 cgcggcggcg atttcgctcg cagccggggc acacgccatg gcgtggatgg cgctgtgaaa
     8221 atcgcgattc aggcgccggt agacggggtc gcgatcctgc gggccaccgg cgttctgcaa
     8281 tgcctggtcg atgcgcgcca gggtctggcc gaacgcgatc aagtcggcct cgctacgccg
     8341 ctgcgccgcc agctgggcaa ccgtgccttc caccgcggca aaggcggcga agaaatcctc
     8401 cacgttgccg gccgtgggcg ccgccacctg gcagccgacc tgcggcacga tgtcgatcaa
     8461 accctcggcc tgcaggcgct tgagggcctc catgaccggc acccgactgc acgcaagtgc
     8521 ctgcgccagt gccagcacgg acagccggtc gccgggacca aggtcgcctt ccaggatgcg
     8581 cgccttcagg tgctggtagg cgtggccggc gcgtggcgcc ttgccgcggc gggcggcgtg
     8641 gcttgaacct tcaactaaca tattagtatt ctaacgaaac gaatgttcgt tattttcggc
     8701 cttgccaacg ttcggctggc ggcccgtgag gaggatgcag gatgaccgac aagatcaggc
     8761 tgggcgcctt tctgctgccc agcgccgggg ccacgattgc gcagtacgag caaggcttcg
     8821 ccggccagaa cccgaatttc taccagaaca cgctgcgcga cagcgcgcgg ctgatccgcc
     8881 aactggacga catcggcttc gacttcgtcg ccttctcgga gcaccacttt cacctggagg
     8941 ggctggagct ttccaacaac ccgatcctgc tgggcgcctg ggcggcgggg ctcacgcgca
     9001 agctgcgcat cggccagatg ggcaacgtgc tgccggcgcg caatccgctg ttgatggccg
     9061 aggacctggc catgctggat cacttctcgg aaggccgcat gatggccggc ttcgcgcgcg
     9121 gatatcaggc ccgccacgtg gccaccatcg ggcagaaatt caatgcctgg tcgaccgccg
     9181 ccaacgaccc gaaataccag gaacacgacc gcaccaaccg cgagctgttc gccgagcact
     9241 acgacatcat ccgtcgcgcc tggcgcgagc cgctgttccg gcaccagggc cagcactggc
     9301 agttgccgcc gccgggcatc ccctggaatc acccggccac gctggacatg gcgccgggca
     9361 tggtcggcgc cgacggcatg ctcgagaagg tcggcatcgc gccgatgacg ttgcaggacc
     9421 cggccacgat cgagagctac atcccgttca cgatgagcac ggccaccctg caatgggcgg
     9481 ccagaaacga ggtcatcccg atcctgttca cgcccatcga cgagcttgtg cgcggctgta
     9541 tcgacgcctt tcagggcgcc gcgcaggagg ccgggcgcca gctcgaatgg ggccgcggcc
     9601 tggggcactt tcgcgaaatc ctgatcgccg acacggacca ggaagccgtc gccctgatgg
     9661 aaaacggcct cggctacgtg tggccgcgct ggcaccactg gttcggcttc aacgaggcgc
     9721 tgcgcaatcc gggcgagcag ggcgagatcg ccaacaccgc cgccaccgtg cgcgagcgcg
     9781 gctattcgct gtgcggctcg gtggacaccg tcacccgcct gctcgaacgc atgctcaaga
     9841 ccctgaacac caacctgatc gtgccgtgga tttcggtcgg cccgatgccg atcgatgccc
     9901 tgctcaagag caacgatctg ctggtggaga aagtgctgcc caggctcggc atcgagctgg
     9961 aacgcttcca gccgacgctg cgacaggaat tcaccggcga cctgtggcgc gatgcctcgc
    10021 agcgctgatc gaacgaggta gtcagacgat gagcattcga accggcgcgc agttcctggc
    10081 gggcctgaag gacgaccgcg aggtctggta cgacggcgag cgcgtgcgcg atgtggcctc
    10141 gttcgagcca ttcgcggcgt cagtcaagtc actggccacg ctgtacgaca tgcagcacga
    10201 cccgcagcac cgcgacatcc tgtccgtgga ttgcccggag tttggcgagc gcatcgcccg
    10261 gccgttccag attccgcgca cgcccgagca gctgcgcctg aagcgcgagg cctaccgcat
    10321 ctgggccgag gccacctgcg gcatgatggg ccgcagcccg gatttcctga acgtgatgat
    10381 ggcctcgctg gccgccaagc gcagcttctt tgccgagttc tccgaagaac gtgcggatgc
    10441 ggtggtggcc tactaccggc acgtggccag caacgatctg ttcctgaccc acgcgctgct
    10501 ggacccgcag ctggacaagg gcaagctgcg ccaccagcag accgacccca ccatcaccct
    10561 gcacgtgaag gagcgcacca aagacggcct gctggtgcac ggcgccaagc gcatcgccac
    10621 cgccgcgccg tacgcggacg agctgctggt gtggcccttc ccgccgacct ttcagccggg
    10681 tgaggagccg tacgccaacg tgtttgcggt gccgatgaac acgccgggcc tgaaaacgat
    10741 ctgccggccg tcgttcgcgc accccggccg gcgcaaggac tacccgttgt ccgcgcagtt
    10801 cgacgaaatg gacgccacgg tggtgttcga caacgtgttg gtgccctggg agcgggtgtt
    10861 cctgcacgag gacattgggt tgctgaaccg catgtaccgc gacacgcgca tgcgcgagct
    10921 gaccgcccac cagaccaacg tgcgcctgga aaccaagctc ggcttcgtgt acgcggtgct
    10981 gctgcgggtg gcgcaggcca gcggcatcga cacgcgcaag gacgtgatcg agatgctggg
    11041 cgaggcggtg atgaacctcg aagccatccg caacaccacc cgctcggccg agctccaggc
    11101 cacgctggac cccgaaaacg gcgtgttgta tccggacctt ttcgccctgc agtgcgggcg
    11161 cgcgctgggc ccggtggtct acccgcgcgc ggtgcagatg atccgcaagg tggccggcgg
    11221 cgggctggtg cagctgccaa actccatgca cgagttcgat tcgccgatcg gcgcggacct
    11281 ggaacgggcg ctgcgcggcg ccggtatcgg tggccgcgac aaggcgcagc tgctgcgcct
    11341 ggcctgggac atctgcggca gcgagttcgg cgcccgccac gagctgtacg aaatgaacta
    11401 cgccggcgag tacggcgcgc tgctggccgg cattcaaagg gagtacgggc gcaagtcgta
    11461 ctatctggac cacctcgacc gcttcatgag cacgctatga gcacgcacca cagtttctgg
    11521 gcggatcttc gcgccggcag cttcagccag gggtatctgg ccgccggcgg catccgcacc
    11581 cgctacctgc atgccggcga gcgcggcatg ccgccgttga tcctgatcca cggcaccggc
    11641 ggccacgccg aggcctacac gcgaaacctg atcgcccacg gcgagcactt cgacacctac
    11701 gccatcgacc tggtcggcca cggctggagc gacaagccgc ttgcgccgta cgagatcagc
    11761 gtctacgtgc ggcacctgcg tgcggtgctc gacacattgg gcttcgaccg cgtgcatctg
    11821 tccggcgagt cgctgggcgg ctgggtcggg gcgcgcttcg cactcgaata cccggaccgg
    11881 gtggaccggc tggtgctgaa caccaccggc ggcgccacgc tggacctggg cgtgatggag
    11941 cgaataaaaa cgctcagcac ggcggcggtg gaaaacccga gctgggagac catcaaggcg
    12001 cgcctggagt ggctgatggc cgacccggcg gtcgtcaccg acgatctggt cgcctgccgc
    12061 caggccatct acgcgcagcc ggccatgcgc gccgccatgc cgcacatcct gtgcctgcag
    12121 gagccggaca tccgccgccg caacaacctg accgacgccg actgggcagc catcaaggcg
    12181 cccacgctgg tgctgtggac ggacaaggac ccgaccgccg cggtgagcgt cggccagcgc
    12241 attgccgacc tgatcccgaa cgcccggctt gaagtgatgg agcgctgcgg ccactggccg
    12301 cagttcgagg acgcgccgcg cttcaatgac ctgcacatcg gctttctcgg cggccggggc
    12361 tagcgtgcca tgccgctggc cgcgctgttc gcgtcccaca cgccgctgaa ggattacctc
    12421 gatccggggc cgggcatcgc cgccgaagtc gacgcgtgcc tggccgaagc tcgtgcctgg
    12481 gtacaggcct tcgcgcccga cctggtgatc gaattcgcgc cggaccattt caacggcttt
    12541 ttctatcagc tgatgccgag cttctgcatc ggcgcggcgg ccgagtcggt cggcgactgg
    12601 aagaccgcca ccggtccgct gccggtggcc ggcgagctgg cacagcggct ggtcgaggcg
    12661 gtgcatgccg acggcgtgga cgtggccctc tcgcagcgca tgacggtgga ccacggcttc
    12721 acgcagctgc tggagatcct gttcgatgcc ggcaaggcgc cgccgctgat tccggtgttc
    12781 atcaactgcg ccgccccgcc gcgcccaccg atggcacgcg
//
LOCUS       DATCUN010000010         6072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_225486, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000010 DATCUN010000000
VERSION     DATCUN010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225486"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /gene="tssI"
                     /locus_tag="VIO83_00300"
     CDS             <1..1623
                     /gene="tssI"
                     /locus_tag="VIO83_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEY8329764.1"
                     /translation="LHFIQRLCEEEGLHYHFQHSTDDHVLVFGDDQTVFPRLGRPSAY
                     VQDSGLVAVEPVVKRFAVRLETRTSRVSRRDYDFAQPRLQLESTFASEFEPDLEDYDY
                     PGRYTERSRGKHLARRALERHRADYQLAEGDSDQPTLRSGHFLELGEHPRREWNQLWL
                     LGEVHHEGKQPQVLEESVTSEVTASDGFQQGYRNRFTATPWDVPFRPPRAHPKPRLLG
                     SQSAVVTGPAGEEIHCDQYGRVKVQFHWDREGQADDTTSCWLRVSSSWAGDRYGGIAI
                     PRVGMEVLVSFLEGAPDQPLVTGCLYHKEHVVPYDLPANKTRSLFKTLSSPGGGGYNE
                     LRIEDRQGQEQIYIHAQRDWDQNIEHDQKLRVGQQRHDSVEANAYSEFKAEEHRTTHG
                     ERRSEVRASDHLTVGASQHVKIGQGRFVEAGSEIHYYAGEKLVIDAGLEFTAKGGGSW
                     LNLDPSGVSLSGATIKINSGGSPDQGSGIAIRSPVLPGAAARDDAGELLQQASPQPLR
                     QRPRLQAPRGICLECWRKALREQHALLTGEQA"
     gene            1620..2483
                     /locus_tag="VIO83_00305"
     CDS             1620..2483
                     /locus_tag="VIO83_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HEY8329765.1"
                     /translation="MNQVAHGKSDETAWEPGHAMTPSHYAIVEQPSITEEGRRYWQGH
                     HLPRMPLMSQPEFAHLRDEGPWLVALGEQPESALATLRHDLGRHSVQGWISSPLSLAD
                     LARHLSDALIAQDQDGMTFLLRIYAPHVLAVLHQRRDCAWHAWLFAPISEWSVEDKGG
                     RLHSHTGGGLGTSPEYVPIILDAPLTEALAIDQQALALLTELERAAPEAFTITCHGER
                     LAQVEQALAEARASGLDHPDDHNLFAALYLLDGQAPSKAPHWPAAMQLVREENYELAR
                     ALETVQENDLS"
     gene            2480..2953
                     /locus_tag="VIO83_00310"
     CDS             2480..2953
                     /locus_tag="VIO83_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HEY8329766.1"
                     /translation="MKNLANNASKRWREIPKAWRFLLLALCSLGLIGTPLLWQYLTPQ
                     GAALYGHNHTDRPIFSYWVNGNWGGNGGVTCCWAIDGNVLKVAWIKDRTGAQVRQGLK
                     EEQRELRIPNPPRKRTDHYLHVHFFPGDQVRLAWSPDHDSPYEGLREPRQSKESQ"
     gene            2950..3459
                     /locus_tag="VIO83_00315"
     CDS             2950..3459
                     /locus_tag="VIO83_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HEY8329767.1"
                     /translation="MKSRVDRNAGALAQLSPAVLTRRWLQLPKALRFLLLALAWLGLL
                     GAPLLWQYLTPQGAALYGHNHTDRPIADYTVNGNWGGNGGMTCCWAIDGEVLEVVWIK
                     SMTGKQFRRGVKEEQRELRIPNPPRKRTDDTLHVHFLPRDQVRLAWSDSHTSPLKGEI
                     NALLGKEAP"
     gene            3456..5213
                     /locus_tag="VIO83_00320"
     CDS             3456..5213
                     /locus_tag="VIO83_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEY8329768.1"
                     /translation="MKACTLDKAQAIAARSGSMTGKSTVALCGRTLHVGFFFDGFARD
                     LEKDRAKNRISNIGRLFLAHPRPESDDTFNAYRKAYFSGLGADYNADGSTIANGAART
                     ASASLGDLPKDLVTDEAIEGAKDTLDGRNWWERLGRDLDELVDKPQKGLKVLKGAAIG
                     SALEGWAPLRDGSITGTLMKTGADTRLEGALARFRKDLNEVKSASDIPLRWIRVSVFG
                     FDFGATLARAFLHALLEEDTPGAELQLVFAGLFDGVDRSAASNPILEHFLPFRNDLDD
                     GGLVPKEVQTVLHLIAAHERRFYRRARLLGRLQRDWREQLMPGVSEDIGGGLAPGEQK
                     PSAELALASLHRMYRAACQAGVPFPLLEQLFEVDVVTAELFVFNDRVHDRSAATLAER
                     YLRSSGHARPGREGFTAHMRLYIRWLAGLWRDYQTSLRTLDEEEDRLHATRFSGGGIS
                     GLLGIPHESDTQRKQRLQRTRAIDEERTVLQQHHGWLEEVDAEARSLRNRHQIYGLRT
                     AGGSLEHFQMQQTLLAEWFATQPARLDPAVRALFACFMHDKLVLSTAQRTARSLSGEN
                     FFAIRGFDLPDSDDDASAA"
     gene            5247..5378
                     /locus_tag="VIO83_00325"
     CDS             5247..5378
                     /locus_tag="VIO83_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HEY8329769.1"
                     /translation="MPRLYPAALLALALCLAGCSGNYKFSDDDYRPLGDPHVENRGN"
     gene            5400..>6072
                     /gene="tagH"
                     /locus_tag="VIO83_00330"
     CDS             5400..>6072
                     /gene="tagH"
                     /locus_tag="VIO83_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014595381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HEY8329770.1"
                     /translation="MELVFEMVAAQQFGPGLLTSKTFTQAGGIIGRAEDCDWVIPDRK
                     RILSGHHAEVSYRDDAFYLTDTSSNGIQLKDSGASLTRGQPQRIEHDSVYCLGDFEIR
                     ARLVRDPALFAGDIGRPLPAGSIIPDDAFLDLDPLTALEQQERGYAEVDELSAILQPP
                     RAQAQQRDYAQIDEENLPVPELVMPRPAPPSRPAAEPERLPAAFWQRFGADLGVSLDG
                     LDDDAR"
BASE COUNT         1154 a         2108 c         1920 g          890 t
ORIGIN      
        1 ctgcacttca tccagcgcct gtgcgaggaa gagggactcc actaccactt ccagcacagc
       61 acggacgacc atgtgttggt gttcggcgac gaccagacgg tgtttccaag gctgggacgg
      121 cccagcgcct atgtgcagga cagcggcctg gtggccgtcg agccggtggt caagcgcttc
      181 gccgtgcgcc tggaaacccg caccagccgg gtcagccgcc gcgactacga cttcgcgcag
      241 ccgcgcctgc agctggagag caccttcgcc agcgagttcg aaccggacct ggaggactac
      301 gactaccccg ggcgctacac tgaacgctcg cgcggcaagc acctggcccg ccgcgccctc
      361 gagcgccacc gcgccgacta ccagctggcc gagggcgaca gcgaccagcc gaccttgcgc
      421 agcgggcact tcctcgagct gggcgagcat ccgcgccgcg agtggaacca gctgtggctg
      481 ctcggcgaag tccaccacga aggcaagcag ccgcaggtgc tggaggagtc ggtcaccagt
      541 gaggtgactg cgagcgacgg cttccagcag ggctaccgca accgcttcac cgccacgccc
      601 tgggacgtgc ccttccgccc gccgcgggcc catcccaagc cgcgcctcct cggcagccag
      661 agcgccgtgg tcaccggccc ggccggcgag gagatccact gcgaccaata cgggcgggtc
      721 aaggtgcagt tccactggga ccgcgagggc caggccgacg acactaccag ctgctggctg
      781 cgggtctcca gcagctgggc cggcgaccgc tacggcggca tcgccattcc gcgggtgggt
      841 atggaagtgc tggtcagctt cctcgaaggc gcccccgacc agccgctggt caccggctgc
      901 ctgtaccaca aggaacacgt cgtcccctac gacctgccgg cgaacaagac ccgcagcctg
      961 tttaagaccc tcagctcccc gggcggtggc ggctacaacg agctgcgcat cgaggaccgc
     1021 cagggccagg agcagatcta tatccatgcc cagcgcgact gggaccagaa catcgagcac
     1081 gaccagaagc tccgcgtcgg ccagcagcgc cacgacagcg tcgaggccaa cgcttacagc
     1141 gagttcaagg ccgaggaaca ccgcaccacc cacggcgagc gcaggagcga agtacgcgcc
     1201 agcgatcacc tgacggtcgg cgccagccag cacgtgaaga tcggccaggg ccggttcgtc
     1261 gaagccggca gcgagatcca ttactacgcc ggcgaaaagc tggtgatcga cgccggcttg
     1321 gagttcaccg ccaagggcgg cggcagctgg ctcaacctcg atccgagcgg cgtcagcttg
     1381 agcggcgcga ccatcaagat caactcgggt ggcagcccgg accagggctc ggggatcgcc
     1441 atcaggtcgc cagtgctgcc gggcgcggca gcccgggacg acgccggaga gctgctgcag
     1501 caggcaagcc cccagcccct tcgacagcgc ccgcgcctgc aggctcccag aggaatatgc
     1561 ctcgaatgct ggcgcaaggc cctgcgtgaa cagcacgccc tgctgaccgg agagcaggca
     1621 tgaaccaggt cgctcacggc aagagcgatg aaactgcatg ggagccgggt cacgcaatga
     1681 cgccaagtca ctatgccatc gttgaacaac cctccatcac cgaggaagga cgacgctact
     1741 ggcaaggcca ccacctgcca cgcatgccac tgatgagcca gccggagttc gcccatctgc
     1801 gcgacgaagg tccgtggctg gtggcactgg gcgagcaacc ggaaagtgca ctggcgaccc
     1861 tgcgccacga tctaggccgg cactcggtac agggctggat ttccagccct ctgtccctgg
     1921 ctgatctggc acgtcacctc agcgatgccc tgatcgccca ggatcaagac ggcatgacct
     1981 ttctgctgcg catttatgcg ccccatgtgc tcgcggtgct gcaccagcgc cgggactgcg
     2041 cctggcatgc ctggctgttc gccccgatca gcgagtggtc ggtggaagac aagggtggcc
     2101 gcctgcattc ccatacaggt ggcggactgg gcacctcgcc cgaatacgta ccgatcatcc
     2161 tggatgctcc gctgacggag gctctggcga tagaccagca agccttggcg ctactgacgg
     2221 agcttgagcg cgccgcaccg gaagccttca ccatcacctg ccatggcgaa cggcttgcac
     2281 aagtggaaca ggcgttggcc gaggcccgcg caagcggact ggatcacccc gacgaccaca
     2341 acctgtttgc cgctctgtat ctgctcgacg ggcaggctcc cagcaaagcg cctcactggc
     2401 cggcagccat gcaactggtg cgggaggaga actacgagct ggctcgtgcg ctggaaaccg
     2461 ttcaggaaaa cgacctctca tgaaaaatct tgcgaacaac gccagcaagc gctggcgaga
     2521 aatccccaag gcatggcgct tcctgctgct agccctgtgc tcactgggcc tgatcggcac
     2581 gcccctgctc tggcagtacc tcaccccgca gggcgcagcg ctgtatggcc ataaccatac
     2641 cgaccgaccg atcttcagtt actgggtgaa tggcaactgg ggaggtaatg gcggggtgac
     2701 ctgctgttgg gccatcgacg gcaacgtgct taaggttgcc tggatcaagg accgaaccgg
     2761 cgcgcaggtg cgccagggcc taaaggagga gcagcgcgag ctgcgcatcc ccaatccgcc
     2821 ccgcaagcgc acggatcatt acctgcacgt gcacttcttc cccggtgatc aggtacgcct
     2881 ggcctggagc ccggaccatg actcgccata cgaaggactc agggaacccc gtcagagcaa
     2941 ggagagccaa tgaaatcccg tgttgaccgc aacgccgggg ctctggcgca gctgtcccca
     3001 gccgtcctga cccggcgctg gctgcaactg cccaaagcgc tgcgcttcct gctgctggcc
     3061 ctggcctggc tggggctgct cggcgccccc ctgctctggc agtacctcac tccgcagggt
     3121 gcggcgctgt atggccacaa ccataccgac cggccgatcg ccgattacac ggtcaatgga
     3181 aactggggcg gcaatggcgg gatgacctgc tgttgggcca tcgacggcga ggtgctcgag
     3241 gttgtctgga tcaagagcat gaccggcaag cagtttcgcc ggggggtcaa ggaggagcag
     3301 cgcgagctgc gcatccccaa tccgccccgc aagcgcacgg acgacaccct gcatgtgcat
     3361 ttcctgccaa gggatcaggt acgacttgcc tggagcgaca gccacaccag tccactgaaa
     3421 ggcgaaatca acgccctgct gggcaaggag gcaccatgaa agcctgcaca ctggacaagg
     3481 ctcaggccat tgcggcacgg agcggatcga tgaccggcaa aagcactgtc gccctttgtg
     3541 gccgcaccct gcatgtgggg ttcttcttcg acggtttcgc ccgcgaccta gaaaaggacc
     3601 gggcgaaaaa ccggatcagc aatatcggtc ggcttttcct tgcccacccg cgcccggaaa
     3661 gcgatgacac cttcaatgcc taccgaaagg cctatttcag cggtctgggc gcggactaca
     3721 acgccgacgg cagcaccatc gccaacggcg ccgcccggac agcctcggcg tccctcggcg
     3781 atctccccaa agacctcgtc accgacgagg ccatcgaagg cgccaaggac accctcgacg
     3841 ggcgcaactg gtgggagcgg ctcggccgcg acctggacga gctggtcgac aagccgcaga
     3901 aaggcctcaa ggtactcaag ggcgcggcta ttggcagtgc gctagaaggc tgggcgccgc
     3961 tgcgcgacgg ctccatcacc ggcacgctga tgaagaccgg cgccgacacc cgcctcgaag
     4021 gggccctggc acgttttcgc aaagacctca atgaagtcaa aagcgccagc gacatcccac
     4081 tgcgctggat cagggtctcg gtattcggct tcgacttcgg tgccaccctg gcgcgcgcct
     4141 tcctgcacgc gttgctcgag gaagacacgc cgggcgccga actgcagctg gtgttcgccg
     4201 gcctcttcga cggtgtcgac cgcagcgcgg ccagcaaccc gattctcgag cacttcctgc
     4261 cgttcaggaa cgatctggac gatggcggac tggtacccaa ggaggtgcag acggtactgc
     4321 acctgatcgc cgcccacgaa cgtcgcttct accggcgcgc ccgcctgctg ggccgcttgc
     4381 agcgtgactg gcgcgagcag ttgatgcccg gggtgagcga ggacatcggc ggcggcctag
     4441 ccccgggcga acagaagccc agcgccgagc tggccctcgc cagcctgcac cggatgtatc
     4501 gggccgcctg ccaggccgga gtgcccttcc cacttctgga gcaattgttc gaggtggacg
     4561 tggtgacagc cgagcttttc gtcttcaacg acagggtgca cgaccgtagc gctgccacct
     4621 tggccgagcg ctacctgcgc agcagcggtc atgcccggcc tggccgagaa ggcttcaccg
     4681 cccacatgcg cctgtatatc cgctggctgg ccggactctg gcgcgactac cagacctcgc
     4741 tgaggactct ggatgaggaa gaggatcgcc tgcatgccac ccgtttttcc ggtggcggga
     4801 tcagcggcct gctcggaatt cctcacgaga gcgacacgca acgaaagcag cgcctgcagc
     4861 gaacccgggc gatcgacgag gagcgcacgg tcctgcagca gcaccatggc tggctggaag
     4921 aagtcgatgc ggaggcgcgc tcgctgcgca atcgccacca gatctatggc ctgcgcacag
     4981 ccgggggcag cctggaacat tttcagatgc agcaaaccct gctcgccgag tggttcgcga
     5041 cccagcctgc ccgactcgat ccggcagtcc gcgcgctgtt cgcctgcttc atgcacgaca
     5101 agctggtgct tagcacggcg cagcgcacgg ccaggtcgct gagcggcgaa aacttcttcg
     5161 cgatccgcgg cttcgacctg ccggatagcg acgacgacgc cagcgccgcc tgaatcgatc
     5221 aattgcccat ggaaaggaac acctgcatgc cccgcctcta ccccgccgcc ctgctcgccc
     5281 tggccctgtg cctggccggc tgcagcggca actacaagtt cagcgacgac gactaccgcc
     5341 cactgggcga tccgcacgtg gaaaaccgcg gcaactgacc gccagggagg acaacaacca
     5401 tggaactggt tttcgaaatg gtcgctgccc agcagttcgg ccccgggctg ctgaccagca
     5461 agaccttcac ccaggccggc ggcatcatcg gccgcgccga ggattgcgac tgggtgatcc
     5521 ccgatcgcaa acgcatcctc tccggccacc atgccgaggt gagctaccgc gacgacgcct
     5581 tctacctcac cgacaccagc agcaacggca tccagctcaa ggacagcggc gccagcctga
     5641 cccgggggca gccgcagcgc atcgagcacg acagcgtcta ctgcctgggc gacttcgaga
     5701 tccgcgcgcg gctggtccgc gatccggcgc tgttcgccgg cgatatcggc cgcccgctgc
     5761 cggccggcag catcattccc gacgatgcct tcctcgatct cgacccgctg accgcgctgg
     5821 agcagcagga gcgcggctat gccgaggtcg acgagctgag cgcgatcctc cagccgccgc
     5881 gggcccaggc gcagcagcgc gactacgcgc agatcgacga ggaaaacctg ccggtaccgg
     5941 aactggtgat gcccaggccc gcgccgccgt ccaggccggc cgccgagccc gagcgcctgc
     6001 ctgccgcctt ctggcagcgc ttcggcgcag acctcggcgt cagcctcgac ggcctcgacg
     6061 acgacgcccg cg
//
LOCUS       DATCUN010000011         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_94080, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000011 DATCUN010000000
VERSION     DATCUN010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94080"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VIO83_00335"
     CDS             complement(<1..333)
                     /locus_tag="VIO83_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY8329771.1"
                     /translation="MSDNNPCLACGACCAHFRVSFYWGECQSAGGIVPDELVEQITPY
                     HAAMRGTTSKPARCVSLLGDVGCGVRCTMYEQRSSPCREFEASWEHGEHNPRCDAARA
                     AHGLPPLTP"
     gene            497..1951
                     /locus_tag="VIO83_00340"
     CDS             497..1951
                     /locus_tag="VIO83_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEY8329772.1"
                     /translation="MALPTLRMIAFILGIFLITLALSMIIPIITLLSMDNPEGLSDFL
                     WPALITAGCGLAMVLPGQPKHAQLRPRDMYMLTTSSWVVVCGFAALPMVFIQHIGYTD
                     AFFETMSGITTTGSTILTGLDHASPALLIWRSMLHWLGGIGFIGMAVAVLPLLRVGGM
                     RLFQTESSDWSQKVMPRSHMVAKYILVIYVAFTLLGFLAFWWAGMTPFEAINHAMAAI
                     STGGFSTSDSSLAHWPQPAVHWVAVVFMLLGSLPFTLYVAFLRGHRKALFRDQQVRGF
                     LGFLLITCLGFGTWLWFHSEHSWLDALRIVTVNVVSVVTTTGFALGDYTLWGGFSILL
                     FFYLTFVGGCSGSTAGGLKIFRFQVAFVMLKANLQQMIHPRSVIKQQYNNHPIDDDIV
                     RSLITFSFFFTITIGVIALGLALIGLDWITALTGAATAVCNVGPGLGPIIGPAGNFAS
                     LPDSAKWLLSVGMLLGRLEIITILVVLTPAFWRH"
     gene            2109..3500
                     /locus_tag="VIO83_00345"
     CDS             2109..3500
                     /locus_tag="VIO83_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8329773.1"
                     /translation="MKLSLFARSAKQLPDPRRREYQSRVLAYLAAAASIAAGTYSQHF
                     GYDILWLVPYTLLYPHLAQRLSQRFKHSHPQQTDLALMVIDALHAGAGMTLLGYSLVP
                     SLMFALTLGFSALIIGGLRSLTIVTLGALCGAVLSFLVVSPALKLNTPPLVSAVSILF
                     TLLYLCIAAFLVHEQAIRLALAREEVKKEQEKAARLASSLAKYLSPQVWESIFSGKKS
                     VRLETQRKKLTVFFSDIKGFTELSEELEAEALTDVLNTYLNEMSKIALKYGGTIDKFI
                     GDAVMIFFGDPTTQGAKKDAVAAVSMAIAMRKHMKVLRQQWRSQGITKPLEIRMGINT
                     GYCTVGNFGADTRMDYTIIGREVNLASRLESASESGEILISHETYSMIKDVIMCRDKG
                     QISVKGFSRPVQIYQVVDFRRDLGASASYLEHELQGFSMYLDTNSIQNYDKERVILAL
                     QQAAEKLRDKVIY"
     gene            3532..>3795
                     /locus_tag="VIO83_00350"
     CDS             3532..>3795
                     /locus_tag="VIO83_00350"
                     /inference="COORDINATES: protein motif:HMM:NF036823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HEY8329774.1"
                     /translation="MSAPKPSLPACCSVPEADWPLPQVLPGLTLHSCRFDPAQLAADD
                     FARSQLTPPPGIASAARKRQSEYLAGRLCAASALRQLSGTPSFP"
BASE COUNT          616 a         1409 c         1110 g          660 t
ORIGIN      
        1 cggggtcagc ggcggcaggc cgtgggcggc gcgagcggcg tcgcagcgcg ggttgtgctc
       61 gccgtgctcc caggaggcct cgaactcgcg gcagggggag gagcgctgct cgtacatggt
      121 gcaacgcacc ccgcagccaa catcacccag caggctgacg cagcgcgccg gcttgctggt
      181 ggtgccgcgc atggccgcat ggtagggagt gatctgctcg accagttcat ccgggacgat
      241 gccgccggca gactggcatt cgccccagta gaaggacaca cgaaagtgcg cgcagcaggc
      301 gccgcacgca aggcagggat tattgtcgga catcggtgca ataccgcgga aaccggcgac
      361 tgccggcggg agccgcggat tctagcgagt ggccaatcgc gctggaaagg ccgtccggca
      421 gaattttttc ccgccgttta cagcacctgc gccgacgcgc agaatgcggc cctttcaagc
      481 ctgagcgcgc accaccatgg ccctgccgac cctgcgcatg attgccttca tcctgggcat
      541 cttcctgatc accctcgcgc tcagcatgat catcccgatc atcaccctgc tgagcatgga
      601 caacccggaa ggtctctccg actttctctg gccggcgctg atcaccgccg gttgcggcct
      661 ggccatggtc ctccccggcc agcccaagca cgcgcagctg cgcccgcgcg acatgtacat
      721 gctgaccacc tccagctggg tggtggtctg cggtttcgcc gccctgccga tggtgttcat
      781 ccagcacatc ggctacaccg acgccttctt cgagaccatg tcggggatca ccaccaccgg
      841 ctcgaccatc ctcaccgggc tcgaccacgc ctcgccggcc ctgctgatct ggcgctcgat
      901 gctgcactgg ctcggcggca tcggcttcat cggtatggcc gtggcggtgc tgccgctgct
      961 gcgcgtcggt ggcatgcgcc tgttccagac cgagtcctcg gactggtcgc aaaaggtcat
     1021 gccccgctcg cacatggtgg ccaagtacat cctcgtcatc tacgtcgcct tcaccctgct
     1081 cggcttcctc gccttctggt gggccgggat gaccccgttc gaggcgatca accacgccat
     1141 ggccgcgatc tccaccggcg gcttctccac ctccgattcc tcgctggcgc actggcccca
     1201 gccggcggtg cactgggtcg ccgtggtgtt catgctgctc ggcagcctgc ccttcaccct
     1261 gtacgtcgcc ttcctgcgcg gccatcgcaa ggcgctgttt cgcgaccagc aggtgcgcgg
     1321 cttcctcggc ttcctgctga tcacctgtct ggggttcggc acctggctgt ggttccactc
     1381 cgagcactcc tggctggacg ccttgcgcat cgtcaccgtc aacgtggtct cggtggtcac
     1441 caccaccggc ttcgccctcg gcgactacac cctgtggggc ggcttctcga tcctgctgtt
     1501 cttctacctg accttcgtcg gcgggtgctc cggctccacc gccggcggcc tgaagatctt
     1561 ccgcttccag gtggccttcg tgatgctcaa ggccaacctg cagcagatga tccacccgcg
     1621 ctcggtgatc aagcagcagt acaacaacca cccgatcgac gacgacatcg tccgctcgct
     1681 gatcaccttc tccttcttct ttaccatcac catcggcgta atcgccctgg gcctcgccct
     1741 gatcggcctg gactggatca ccgcgctgac cggcgccgcc accgcggtat gcaacgtcgg
     1801 ccccggcctc ggcccgatca tcggcccggc cggcaacttc gccagcctgc cggacagcgc
     1861 caagtggctg ctgtcggtcg gcatgctgct cggccgcctg gaaatcatca ccatcctggt
     1921 cgtgctgacc cccgccttct ggcggcacta ggccaccagt gacagcatgt cgcagctggc
     1981 gcgggtactt tgcgcccggc cggcggctcg gctaagatcc gccgtcggcc acggctcgcc
     2041 agtggcccgt ccagcctgca gcacccgaac tagaggtgcg ggcacctgtc agggaagtcc
     2101 tcggccagat gaaactctca ctcttcgcgc gttcggcaaa acaactgccg gaccctcgcc
     2161 ggcgcgaata ccaatcgcgc gtgctggcct atctggccgc cgccgccagc atcgctgccg
     2221 gcacctacag ccagcacttc ggctacgaca tcctctggct ggtgccctac accctgctct
     2281 acccgcacct ggcccagcgt ctgagccagc gcttcaagca cagccacccg cagcagaccg
     2341 acctggccct gatggtgatc gacgccctgc acgccggcgc cggcatgacc ctgctcggct
     2401 attcgctggt gcccagcctg atgttcgccc tgaccctggg cttcagcgcc ctgatcattg
     2461 gcggcctgcg ctcgctgacc atagtcaccc tcggcgccct gtgcggtgcc gtgctcagct
     2521 tcctggtggt cagccccgcg ctcaagctga acaccccgcc gctggtttcg gcggtcagca
     2581 tcctgttcac cctgctttat ctgtgcatcg ccgccttcct cgtccacgag caggccatcc
     2641 gcctggccct ggcccgcgag gaggtcaaga aggagcagga aaaggccgca cgcctggcca
     2701 gcagcctggc caagtacctg tcgccgcagg tgtgggagtc gatcttcagc ggcaagaaga
     2761 gcgtgcgcct ggaaacccag cgcaagaagc tcaccgtgtt cttctccgac atcaagggct
     2821 tcaccgagct gtccgaggag ctggaagccg aggcgctgac cgacgtgctc aacacctacc
     2881 tcaacgagat gtcgaagatc gccctgaaat acggcggcac catcgacaag ttcatcggcg
     2941 atgcggtgat gatcttcttc ggcgacccca ccacccaggg cgccaagaag gatgccgtag
     3001 cggccgtgtc catggccatc gccatgcgca agcacatgaa ggtgctgcgc cagcagtggc
     3061 gcagccaggg catcaccaag ccgctggaaa tccgcatggg catcaacacc ggctactgca
     3121 ccgtgggcaa cttcggcgcc gacacgcgca tggactacac catcatcggc cgcgaggtga
     3181 acctcgccag ccgcctggaa agcgcctccg agtccggcga gatcctcatc tcccacgaga
     3241 cctactcgat gatcaaggac gtgatcatgt gccgcgacaa gggccagatc agcgtcaagg
     3301 gcttctcccg gccggtgcag atctaccagg tggtcgactt ccgccgcgac ctcggcgcct
     3361 ccgccagcta cctggagcac gaactgcagg gcttctccat gtacctggat accaacagca
     3421 tccagaacta cgacaaggaa cgggtcatcc tcgccctgca gcaggccgcc gagaagctgc
     3481 gcgacaaggt catctactga cccggccgcg cggctgcgcc atactcaccg catgtccgcc
     3541 ccgaaaccct ccctgcccgc ctgctgcagc gtccccgaag ccgactggcc gctgccacag
     3601 gtgctgcccg gcctgaccct gcacagctgc cgtttcgacc ctgcacagct cgccgccgac
     3661 gacttcgccc gcagccagct gacgccgccg cccggcatcg ccagcgccgc ccgcaagcgc
     3721 cagagcgaat accttgccgg gcgcctgtgc gccgccagtg ccctgcgcca gctcagcggc
     3781 acacccagct tcccg
//
LOCUS       DATCUN010000012         3834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_19031, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000012 DATCUN010000000
VERSION     DATCUN010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19031"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VIO83_00355"
     CDS             <1..217
                     /locus_tag="VIO83_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450283.1"
                     /note="catalyzes the formation of pyruvate and
                     acetaldehyde from 4-hydroxy-2-ketovaleric acid; involved
                     in the degradation of phenylpropionate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HEY8329775.1"
                     /translation="RVDRDALTLGYAGVYSSFLLFAQRAEKKYGVPARDILVELGRRG
                     TVGGQEDMIEDLALDMAKARQNLKVTA"
     gene            214..1008
                     /gene="dmpH"
                     /locus_tag="VIO83_00360"
     CDS             214..1008
                     /gene="dmpH"
                     /locus_tag="VIO83_00360"
                     /EC_number="4.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047437 - 4-oxalocrotonate decarboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-3-hexenedioate decarboxylase"
                     /protein_id="HEY8329776.1"
                     /translation="MNRTLTREQVLALAEHVEGAELNVHDIPKITNDYPEMTFADAYD
                     IQWEIRRRKEARGNKTVGLKMGLTSWAKMAQMGVETPIYGFLSDYFSVPDGGVIDCSK
                     LVHPKIEAEISVVTKAPLRGPGCHIGDVIAAVDYVIPTVEVIDSRYENFKFDLISVVA
                     DNASSTRYITGGQMANLEDVDLRTLGVVMEKNGEVVEVGAGAAVLGHPLSSVAMLANL
                     LAERGEHIPAGTFIMTGGITAAVAVEPGDNVTVRYQGLGSVSARFV"
     gene            1059..1250
                     /locus_tag="VIO83_00365"
     CDS             1059..1250
                     /locus_tag="VIO83_00365"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEY8329777.1"
                     /translation="MPIAHVQIMEGRSDEQKEAMIREVSEALARTLDSPLDRVRVLIT
                     ELPKSHWGIGGTPASKLGR"
     gene            complement(1343..1603)
                     /locus_tag="VIO83_00370"
     CDS             complement(1343..1603)
                     /locus_tag="VIO83_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329778.1"
                     /translation="MTDEQYRHESLSVLREIRDGQHEILAQLAAQRALAEQQLTRSRE
                     KVDESIALQKLALQRQRTITLIAVPGILACIAAIAYLLLRYF"
     gene            complement(1634..2137)
                     /locus_tag="VIO83_00375"
     CDS             complement(1634..2137)
                     /locus_tag="VIO83_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329779.1"
                     /translation="MSKLFRNTLYLQLQSDKLRALHVESGRAFAEAPVLAWRSEKGRV
                     IPLAAGNAAQVLRGRPGVTVVNGFQHPRSLLADFSVAERTLQLLFKTFIPATLFQAAP
                     TVILHPLEHLEGGLTQVEIRGLAELLLGAGARRAFIWSGAELSRTALESLQFPAEGGT
                     LHYPEPS"
     gene            complement(2134..2577)
                     /locus_tag="VIO83_00380"
     CDS             complement(2134..2577)
                     /locus_tag="VIO83_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329780.1"
                     /translation="MLPKDPLARLLFKLGLLPLAWFAFLFWFENFAIWGSRASSDAPA
                     GGTTAHLMSLIHESGNPGYAPYGLHVFLAPAWLPLPQRFGELAFAGYCKGSAEIAWQA
                     ADRLVIRCEARKIVTPPSSRRGIRVELDSRPRSSPLHPQGNGSAR"
     gene            complement(2589..2942)
                     /locus_tag="VIO83_00385"
     CDS             complement(2589..2942)
                     /locus_tag="VIO83_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329781.1"
                     /translation="MRNLILLLALCGGGYKAWEHFSRESNAPLHAEPYLAVYGRDACG
                     YTQTTLRQLDQAGIRYQYHSVDSPAVAERLHRRMQNAGLEVRRYLLPVVDLNNRITVH
                     PDNGELVAQARQLLR"
     gene            complement(3026..3514)
                     /locus_tag="VIO83_00390"
     CDS             complement(3026..3514)
                     /locus_tag="VIO83_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016703619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8329782.1"
                     /translation="MNTDLSFRPVRDDDLPAICTFARTPEELYFLAPRASFPLDVEQL
                     ATSIALRSDSSVVYRGEQVLAFANIYKREQGACYVGNVAVSPQARRQGVARFLMQQMA
                     RLAAERYGASELRVSCFNPNTAGLLLYARLGYTPFALEERLDSRQQPIVLIHLRLPLA
                     SA"
BASE COUNT          637 a         1240 c         1345 g          612 t
ORIGIN      
        1 ccgcgtcgac cgcgatgccc tgaccctggg ctacgccggc gtgtacagct cgttcctgct
       61 gttcgcccag cgcgccgaga agaagtacgg cgtgccggcc cgtgacatcc tcgtagagct
      121 tggccgccgc ggtaccgtcg gcggccagga agacatgatc gaagacctgg ccctcgatat
      181 ggccaaggcc cgtcagaacc tgaaggtgac tgcatgaacc gtaccctgac ccgcgaacag
      241 gtgctggccc tggccgagca cgtcgaaggc gccgagctga atgtccacga cattccgaag
      301 atcaccaacg actacccgga gatgaccttc gccgacgcct acgacatcca gtgggaaatc
      361 cgtcgccgca aggaagcccg cggcaacaag accgtcggct tgaagatggg tctgacctcc
      421 tgggcgaaga tggcgcagat gggcgtggag acgccgatct acggcttcct ctccgactac
      481 ttcagcgtgc cggacggcgg cgtgatcgac tgctccaagc tggtccaccc gaagatcgag
      541 gcggaaatct ccgtggtcac caaggcgccg ctgcgcggtc cgggctgcca catcggcgac
      601 gtgatcgccg cggtcgacta cgtgatcccg accgtggaag tgatcgactc gcgctacgag
      661 aacttcaagt tcgacctgat cagcgtggtg gccgacaacg cctcttcgac ccgctacatc
      721 accggcggcc agatggccaa cctcgaggac gtcgacctgc gtaccctcgg cgtggtgatg
      781 gagaagaacg gcgaagtggt ggaagtcggc gccggcgccg cggtgctcgg ccatccgctg
      841 tccagcgtgg ccatgctggc caacctgctg gccgagcgcg gcgagcacat cccggccggc
      901 accttcatca tgaccggcgg catcaccgcc gccgtggcgg tggagccggg cgacaacgtc
      961 accgtgcgct accagggcct gggcagcgtc tccgcccgct tcgtctgagt cacctgcccc
     1021 ggccgctggc cggggccttc tttgcgagga ggtttgtcat gccgattgcc catgtccaga
     1081 tcatggaagg ccgcagcgac gagcagaagg aagcgatgat ccgcgaggtc agcgaggcgc
     1141 tggcgcgcac cctcgattcc ccgctcgacc gcgtgcgcgt actgatcacc gagctgccga
     1201 agagccactg gggcatcggc ggcacgccgg cgagcaagct cggccgctga gctgagcgct
     1261 ccaccagagc aagaccacca agcccggcga tgccgggctt tgctgtttct ggggaaggag
     1321 tgttccacgt ggaacagcat ggctagaagt agcgcagcag cagataggcg atcgccgcga
     1381 tgcaggcgag gatgcccggc accgcgatca gggtgatcgt gcgctggcgc tgcagggcca
     1441 gtttctgcag ggcgatggac tcgtccacct tctcccgcga gcgcgtgagc tgctgttccg
     1501 ccagcgcccg ctgcgcagcg agctgggcga ggatctcgtg ctggccatcg cggatctcgc
     1561 gtagcacgct gagggattcg tggcggtact gctcgtcggt catgcgctcc atccttgccg
     1621 ctcggcactg gtttcagctg ggttcgggat agtgcaatgt gccaccttcg gccgggaact
     1681 gcaggctctc cagcgccgtc cggctcaact ccgccccgct ccagatgaat gcccgccgtg
     1741 ccccggcgcc gagcagcagt tcggccaggc cgcggatctc gacctgggtc aggccgccct
     1801 cgaggtgctc cagcggatgg aggatcacgg tcggggcggc ctggaacagc gtcgccggta
     1861 tgaaggtctt gaacagcagc tgcagggtcc gctccgccac gctgaagtcc gccagcaggc
     1921 tgcgcggatg ctggaagccg ttgaccaccg tcacccctgg gcgcccacgc aggacctgcg
     1981 ccgcgttgcc ggccgccagc gggatcaccc tgcccttctc gctgcgccag gccagtaccg
     2041 gcgcctccgc gaatgcccgg ccggactcca catgcagtgc ccgcagcttg tccgactgca
     2101 gctgcaggta gagggtgttg cggaacagtt tgctcatctg gcgcttccgt tgccttgtgg
     2161 gtggaggggg gatgatcggg gccggctgtc gagctcgacg cggataccgc gccggctgct
     2221 cggcggggtc acgatcttgc gggcctcgca gcggatgacc agccggtcgg ccgcctgcca
     2281 ggcgatttcc gcgctgccct tgcagtagcc ggcaaaggcc agctcgccga agcgctgcgg
     2341 cagtggcagc cacgccgggg cgaggaacac gtgcaggcca tagggcgcat agcccgggtt
     2401 gccgctttcg tggatcaggc tcatcaggtg ggcggtcgtg ccgcctgcgg gggcgtcgct
     2461 gctggcccga cttccccaga tggcgaagtt ctcgaaccag aacaggaagg cgaaccaggc
     2521 gagcggcagc aggcccagct tgaacaggag gcgggccagc ggatccttgg gcagcatggg
     2581 tgtcccgctc agcgcagcag ctggcgcgcc tgcgccacca gctcaccgtt gtccggatgc
     2641 accgtgatcc ggttgttcag gtcgaccacc ggcagcagat agcgcctgac ctcgagaccg
     2701 gcattctgca tgcgccggtg caggcgctcg gccaccgccg ggctgtccac cgagtggtac
     2761 tggtagcgga tgccggcctg atcgagctgg cgcagggtgg tctgggtgta gccgcaggcg
     2821 tcgcggccgt agacggccag atagggctcg gcgtgcagcg gggcgttgct ctcgcgggag
     2881 aagtgctccc aggccttata gccgccgcca cacagggcga gcagcaggat gagattgcgc
     2941 atggacaagt ccgtttgtcg acggcggagg tggcactcta gcagcccggt ccggcgcggg
     3001 ctgcgcgccg gttggcagag tgcgctcagg ccgacgcgag cggcaggcgc aggtggatca
     3061 gcacgatggg ctgctggcgg ctatccagcc tttcctccag ggcaaacggc gtatagccga
     3121 gccgggcgta cagcagcagg ccggcggtgt tggggttgaa acaggacacc cgcagctcgc
     3181 tggccccgta gcgctcggcg gccaggcggg ccatctgctg catcagaaag cgcgccacgc
     3241 cctggcggcg cgcctgcggc gacaccgcga cgttgccgac gtagcaggca ccctgctcgc
     3301 gcttgtagat gttggcgaag gccagcacct gctcgccgcg gtagaccaca ctggagtcgc
     3361 tgcgcagggc gatgctggtc gccagctgct ccacgtcgag cgggaagctg gcgcgcgggg
     3421 cgaggaaata cagctcctcg ggtgtgcggg cgaaggtgca gatggcgggc aggtcgtcgt
     3481 cgcggaccgg acggaaactg aggtcggtgt tcatcctgaa ctcctgggtg ggtcgtcagt
     3541 gggaatctaa cacggcggac ggccactgat catcgccggg caatgggggg tctgtggaca
     3601 cgttttgggg cgcttatcca caggccgttt tgtctggcag gccatgcggc tgagaaagta
     3661 gttgtggata aaaaattgtc cacagcatcc ctcaccgctt gcggatcagc ccgggcgagt
     3721 ttcagggcag cggtttgtcg cggtgccgca gccagtagaa gaagccgccg atgccgccta
     3781 gcagaaagcc cagcgggccg gtgatcaaca ggccgagcag cggtccctgg ttgg
//
LOCUS       DATCUN010000013         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_56531, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000013 DATCUN010000000
VERSION     DATCUN010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56531"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..356)
                     /locus_tag="VIO83_00395"
     CDS             complement(57..356)
                     /locus_tag="VIO83_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon YbgE family protein"
                     /protein_id="HEY8329783.1"
                     /translation="MRVTAEVRPWLRRGASRAVSLLLAAPLALLLLIHPAAMLDGDGR
                     YSHGLLMLAMWGISSAFIHGVGFDPYSRVWRTVFHPLGGWLLLGLGYLLLWRAQG"
     gene            complement(340..456)
                     /gene="cydX"
                     /locus_tag="VIO83_00400"
     CDS             complement(340..456)
                     /gene="cydX"
                     /locus_tag="VIO83_00400"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HEY8329784.1"
                     /translation="MWYFTWILGVLLACSFGIVNALWLEATQRLDEGDAGDR"
     gene            complement(588..1730)
                     /gene="cydB"
                     /locus_tag="VIO83_00405"
     CDS             complement(588..1730)
                     /gene="cydB"
                     /locus_tag="VIO83_00405"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY8329785.1"
                     /translation="MFDYETLKLLWWVLIGVLLIGFALTDGFDMGAMALMPFVGKTDN
                     ERRVAINTIAPHWDGNQVWFITAGGALFAAWPMVYAVAFSGLYWAMLLVLFGLFCRPV
                     GFDYRSKLENRQWRSAWDWALFVGGAAPALLFGVAFGNLFLGLPFRLDELMRSSYEGS
                     FFALLHPFALLAGVVSLSMLCAHGGAWLMLRTDAALHARSRQATRLCALAYLLAFAGA
                     GLWLVMGIQGFSLVGGFDDLGAALNPLHKQVALDNGGWLANYDRYPLTQLAPLAGLAG
                     GLLALLGAQLNRGGIAFLGSSLAIVGTICTAGFALFPFVLPSTLDPASSLTVWDAVSS
                     RKTLGIMLVVAGIFVPLILAYTLWCYLRMWGRLNDKIIEANPHGLY"
     gene            complement(1741..>2983)
                     /locus_tag="VIO83_00410"
     CDS             complement(1741..>2983)
                     /locus_tag="VIO83_00410"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY8329786.1"
                     /translation="TFIGLFFFGWDRLSKVQHLAVTWLVALGSNLSALWILIANGWMQ
                     NPVGAEFNYQTMRMELVDFGALLFNPVAQVKFVHTVSAGYVTGAVFVLAISSYYLLKQ
                     RDLGFARRSFAIAAAFGMASILSVIILGDESGYEIGDVQKTKLAAIEAEWETEPAPAA
                     FTLFGLPNQEQQRTDYAVKIPYLMGIIATRSLDEEVKGLKDLIAEHELRIRNGIGAYA
                     LLEKLRAGDQSSETIAAFDAVKQDLGYGLLLKKYTAKVVDASEAQIRQAALDSIPHVA
                     GLFWTFRAMVFSGFLMLALFALAFWASIRRNAENKPWLLRFALCSLPLPWIAAQTGWY
                     VAENGRQPWSIAEVLPTHLSASSLSTGDLWGSLVALVAFYSLLLVIELYLMVKFARLG
                     PSSLHSGRYHFERQAAQPAHA"
BASE COUNT          541 a          990 c         1056 g          396 t
ORIGIN      
        1 cgctggagct gtggagctgc gcgccgcccg gcgccggccg ttgaatgccc ggcgcttcag
       61 ccctgcgccc gccagaggag cagatagccc agcccgagca gcagccagcc gcccagcgga
      121 tggaagacgg tgcgccacac acggctgtac gggtcgaagc cgacgccgtg gatgaaggcg
      181 ctggagatgc cccacatcgc cagcatcagc aggccgtggc tgtagcggcc gtcgccgtcg
      241 agcatcgccg ccgggtggat cagcagcagc agcgccagcg gcgcggccag cagcagggaa
      301 acggcgcggc tggcgccgcg ccgcagccag gggcggacct cagcggtcac ccgcatcgcc
      361 ctcgtcgagc ctctgggtgg cttccagcca cagcgcgttg acgatgccga agctgcaggc
      421 cagcagcacg ccgagaatcc aggtgaagta ccacatgtgt cgttctcctt ggcccaggtg
      481 ggcactgatc gagtagggtg cgccgtgcgc accgggaaac tgccgcggcc catggtgcgc
      541 atggcgcacc ctacgaactc ggcgcgggtt cgcgacagtt cgcagcctca gtacaacccg
      601 tgcgggttgg cctcgatgat cttgtcgttc agccggcccc acatgcgcag gtagcaccac
      661 agggtgtagg cgaggatcag cggcacgaag atccccgcca ccaccagcat gatgcccagg
      721 gtcttgcggc tggacaccgc gtcccacacg gtcaggctgg acgctggatc gagggtcgag
      781 ggcagcacga aggggaacag cgcaaagccc gcggtgcaga tcgtgccgac gatggccagg
      841 ctgctgccga ggaaggcgat gccaccgcgg ttgagctgcg cgccgagcaa cgccagcagg
      901 ccgccggcca ggcccgccag cggggcgagc tgggtcagcg ggtagcggtc gtaattggcc
      961 agccagccgc cgttgtccag ggcgacctgc ttgtgcagcg ggttgagcgc cgcgcccaga
     1021 tcgtcaaagc cgccgaccag gctgaagccc tggatgccca tgaccagcca caggccggcg
     1081 ccggcgaagg ccagcaggta ggccagcgcg cacagccggg tggcctggcg cgaacgggca
     1141 tgcagggcgg catcggtgcg cagcatcagc caggcgccac cgtgggcgca gagcatgctc
     1201 aggctgacca ccccggcgag cagggcgaac gggtgcagca gggcgaagaa cgagccctcg
     1261 taggacgagc gcatcagttc atcgaggcgg aacggcaggc cgaggaacag gttgccgaag
     1321 gccacgccga acagcagcgc cggggccgcg ccgccgacga acagcgccca gtcccaggcg
     1381 ctgcgccact gccggttctc cagcttgctg cggtagtcga agccgaccgg gcggcagaac
     1441 aggccgaaca gcaccagcag catcgcccag tacaacccgg agaaggccac cgcgtagacc
     1501 atcggccagg cggcgaacag cgcgccgccg gcggtgatga accacacctg gttgccgtcc
     1561 cagtgcgggg cgatggtgtt gatcgccacc cggcgctcgt tgtcggtctt gccgacgaag
     1621 ggcatcagcg ccatggcgcc catgtcgaag ccgtcggtga gggcgaaacc gatcagcagc
     1681 acgccgatca gcacccacca gagcagtttc agggtttcgt agtcgaacat gacggtttcc
     1741 tcaggcgtgg gccggctggg cggcctggcg ttcgaagtgg tagcggccgc tgtgcaggct
     1801 ggacgggccg aggcgggcga acttgaccat caggtacagc tcgatcacca gcagcaggct
     1861 gtagaaggcc accagcgcga ccagcgaacc ccacaggtcg ccggtcgaca ggctggacgc
     1921 cgacaggtgg gtcggcagta cctcggcgat cgaccacggc tggcggccgt tctcggccac
     1981 gtaccagccg gtctgcgcgg cgatccacgg cagcggcagg ctgcacaggg cgaagcgcag
     2041 cagccacggc ttgttctcgg cgttgcgccg aatcgaggcc cagaaggcca gggcgaacag
     2101 cgccagcatc aggaagccgg agaacaccat ggcgcggaag gtccagaaca ggccggcgac
     2161 gtgggggatg ctgtccagcg ccgcctggcg gatctgcgcc tcgctggcgt cgaccacctt
     2221 ggcggtgtac ttcttcagca gcaggccgta gcccagatcc tgcttcaccg cgtcgaaggc
     2281 ggcgatggtc tcgctgctct ggtcgccggc gcgcagtttt tccagcaggg cgtaagcgcc
     2341 gatgccgtta cggatgcgca gctcgtgctc ggcgatcagg tccttgaggc ccttgacctc
     2401 ctcgtccagc gagcgggtgg cgatgatgcc catcaggtag ggaatcttca ccgcgtagtc
     2461 ggtgcgctgc tgctcctggt tgggcaggcc gaacagggtg aaggcggccg gcgccggctc
     2521 ggtctcccac tcggcctcga tggccgccag cttggtcttc tgcacgtcgc cgatctcgta
     2581 gcccgactcg tcgccgagga tgatcaccga gaggatcgag gccatgccga aggccgcggc
     2641 gatggcgaag gagcggcggg cgaagcccag atcgcgctgc ttcagcaggt agtagctgga
     2701 gatggccagg acgaacaccg cgccggtgac gtagccggcc gacacggtat gcacgaactt
     2761 gacctgcgcc accggattga acagcagcgc gccgaagtcg accagctcca tgcgcatggt
     2821 ctggtagttg aactcggcgc ccaccgggtt ctgcatccag ccgttggcga tcaggatcca
     2881 cagcgccgac aggttggagc ccagcgccac cagccaggtc accgccaggt gctgcacctt
     2941 gctcaggcgg tcccagccga agaagaacag gccgatgaag gtc
//
LOCUS       DATCUN010000014         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_112978, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000014 DATCUN010000000
VERSION     DATCUN010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112978"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /gene="trpD"
                     /locus_tag="VIO83_00415"
     CDS             complement(<1..175)
                     /gene="trpD"
                     /locus_tag="VIO83_00415"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249341.1"
                     /note="Catalyzes the conversion of
                     N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to
                     anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY8329787.1"
                     /translation="MEIKEALNRVAGNLDLSTAEMQDVMRLIMTGQCSDAQIGAFLMG
                     LRMKSETIDEIVGA"
     gene            complement(175..768)
                     /locus_tag="VIO83_00420"
     CDS             complement(175..768)
                     /locus_tag="VIO83_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY8329788.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELNAEVKVVRNDELSVEQIRALNP
                     ERIVVSPGPCTPNEAGVSLAVIREFAGKLPLLGVCLGHQSIGQAFGGEVVRARVAMHG
                     KTSPVYHRDLGVFEGLANPLTVTRYHSLVVRRESLPDCLEITAWTQLEDGSVDEIMGL
                     RHKTLNVEGVQFHPESILTEQGHELLANFLKQSGGVR"
     gene            complement(781..2259)
                     /gene="trpE"
                     /locus_tag="VIO83_00425"
     CDS             complement(781..2259)
                     /gene="trpE"
                     /locus_tag="VIO83_00425"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEY8329789.1"
                     /translation="MTREEFLRLAAAGYNRIPLARETLADFDTPLSIYLKLADAPNSY
                     LLESVQGGEKWGRYSIIGLPSRSVLRVHGHQARVTVDGVETEVHDCADPLAFVEQFKA
                     RYHVPTLAGLPRFNGGLVGYFGYDSVRYVEQRLGACPNPDPLGTPDILLMVSDAVVVF
                     DNLAGKLHAIVLADPAEADAYAQGLARLDALVARLRQPITPRLGVDLNAPLGKEPAFR
                     SSFSHDDFENAVRAIKEYILAGDCMQVVISQRMSIPFQAAPIDLYRALRTINPTPYMY
                     FFNFGDFHVVGSSPEVLVRVEDGLVTVRPIAGTRPRGASEEADLALEKDLLSDTKELA
                     EHLMLIDLGRNDVGRVSSTGSVKVTEQMVIERYSNVMHIVSNVTGQLKPELSAMDALR
                     AILPAGTLSGAPKVRAMEIIDELEPVKRGVYGGAVGYLAWNGNMDTAIAIRTAVIKDG
                     ELHVQAGAGIVADSVPALEWEETLNKRRAMFRAVALAEQSAE"
     gene            complement(2326..2409)
                     /locus_tag="VIO83_00430"
                     /pseudo
     CDS             complement(2326..2409)
                     /locus_tag="VIO83_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          415 a          805 c          840 g          423 t
ORIGIN      
        1 ccgcgccgac gatctcgtcg atggtctcgc tcttcatgcg caggcccatc aggaaggcgc
       61 cgatctgcgc gtcgctgcac tggccggtca tgatcaggcg catcacgtcc tgcatttccg
      121 cggtgctcag gtcgaggttg ccggccaccc ggttgagggc ttccttgatc tccatcagcg
      181 cacgcctccg ctctgcttca agaagttggc cagcagctcg tggccctgtt cggtgaggat
      241 ggattcggga tggaactgca cgccctcgac gttgagggtc ttgtggcgca ggcccatgat
      301 ctcgtcgacg ctgccgtcct cgagttgggt ccaggcggtg atttccaggc agtccggcag
      361 gctctcgcga cgcaccacca gcgagtggta gcgggtcacg gtcagcggat tggccaggcc
      421 ttcgaacacc cccagatcgc ggtggtagac cggactggtc ttgccgtgca tggccacccg
      481 ggcgcgcacc acctcgccgc cgaaggcctg gccgatgctc tggtggccga ggcagacgcc
      541 gagcagcggc agcttgccgg cgaactcgcg aatcaccgcc agcgacacgc cggcctcgtt
      601 gggcgtgcac ggcccggggg aaaccacgat gcgctcagga ttcagcgcgc ggatctgctc
      661 cacgctcagc tcatcgttgc gcaccacctt gacctcggca ttcagttcgc cgaagtactg
      721 caccaggttg taggtgaagg agtcgtagtt gtcgatcatc agcagcatgt cagtacccct
      781 tcactctgca ctttgttcgg ccagggccac cgcgcggaac atggcgcggc gcttgttgag
      841 ggtttcttcc cactccagcg cgggcaccga gtcggcgacg atgccggcgc cggcctgcac
      901 gtgcagctcg ccgtccttga tcaccgcagt gcgaatggcg atggcggtgt ccatgttgcc
      961 gttccacgcc aggtaaccca ccgcaccgcc gtagacgccg cgcttgaccg gctccagctc
     1021 gtcgatgatc tccatggcgc ggaccttcgg cgccccggac agggtgccgg ccggcaggat
     1081 ggcgcgcagc gcgtccatcg ccgacagctc gggtttgagc tggccggtga cgttggagac
     1141 gatgtgcatc acgttcgagt agcgctcgat gaccatctgc tcggtgacct tcaccgagcc
     1201 ggtgctggac acccgaccga cgtcgttgcg gcccaggtcg atcagcatca ggtgctcggc
     1261 cagctccttg gtgtcggaca gcagatcctt ttccagcgcc aggtcggctt cctcgctcgc
     1321 gccacgcggg cgggtgccgg cgatcggccg cacggtgacc aggccgtcct cgacgcgcac
     1381 cagcacctcg ggcgagctgc cgaccacgtg gaagtcgccg aagttgaaga agtacatgta
     1441 cggcgtcggg ttgatggtac gcagcgcgcg gtacaggtcg atgggcgcgg cctggaacgg
     1501 gatcgacatg cgctgggaga tcaccacctg catgcagtcg ccggcgagga tgtactcctt
     1561 gatggcacgc accgcgttct cgaagtcgtc atggctgaag ctggagcgga acgccggctc
     1621 cttgcccagc ggggcattga ggtcgacacc caggcgcggc gtgatcggct ggcgcaggcg
     1681 ggcgaccagc gcatccagcc gggccagacc ctgggcgtag gcatccgcct cggccgggtc
     1741 ggcgaggacg atggcgtgca gcttgccggc caggttgtcg aacaccacca ccgcgtcgga
     1801 gaccatcagc aggatgtccg gggtgcccag cggatcgggg ttgggacagg cgccgaggcg
     1861 ctgctcgaca taacggacgc tgtcgtagcc gaagtagccg accaggccgc cgttgaagcg
     1921 cggcaatccg gcgagagtcg gcacgtggta gcgcgccttg aactgctcga cgaaagccag
     1981 agggtctgca cagtcgtgga cttcggtctc gacgccgtcg accgtcaccc gcgcctggtg
     2041 gccatgcacg cgcagcacgc tgcggctggg caggccgatg atcgagtaac gcccccactt
     2101 ctcgccgccc tgcaccgatt cgagcaggta ggagttgggc gcgtcggcca gcttcaggta
     2161 gatggacagc ggggtgtcga agtcggcgag ggtttcgcgg gcaagcggaa tgcggttgta
     2221 gccggcagcg gccaaacgca ggaattcttc gcgggtcatg atcagcctcg gggcctggaa
     2281 aacggtcgat atgcaaacgc gccggcaagc cggccaggga cgatgtcagg cgcgccagcg
     2341 ccagcgggcc agagccttga tgaccttcat ccacaatttg caggtgacca ccacgatctg
     2401 atctcttcgg cggggtttga acacgtgcag gcacactagc gcagcactgc gcacgaatca
     2461 accaactggc gcagatcgtc gag
//
LOCUS       DATCUN010000015        16848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_206861, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000015 DATCUN010000000
VERSION     DATCUN010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 16848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16848
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206861"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..753
                     /locus_tag="VIO83_00435"
     CDS             382..753
                     /locus_tag="VIO83_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329790.1"
                     /translation="MVTIRTRKNKDGSVMYTAQLRIKRDGVQVYQESASFSRRKAVEA
                     WAKRRETELSEPGALEREQCGGGATLVEMIERYIEESDPTNPLGKTKKATRAPWPPAT
                     WATWFAGSISTSPVSACWCGT"
     gene            1009..1209
                     /locus_tag="VIO83_00440"
                     /pseudo
     CDS             1009..1209
                     /locus_tag="VIO83_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1404..3065)
                     /locus_tag="VIO83_00445"
     CDS             complement(1404..3065)
                     /locus_tag="VIO83_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8329791.1"
                     /translation="MLSRLKIGTRLGVAFGAIALLLAAATAVGLQGLAAQKRTANEML
                     RIDVALSSNAAEVRRLSLEGRRAEKDIFLNVNNPQAVQTHKQHWDASQQALKNTLEAG
                     AALSPDAELRGLYLQSATLLDAYRNGFGTVYGRIEAGEITDPSIADMVFGQFNDEIQQ
                     LDKLAADIDQAAVKLLDGAGEQIARQHHNALIGLLAFSALALLIAAIMAVRITRSISA
                     PLHSALEATRRVADGDLTQDIFGNPHDETGQLLEAMGETNQKLSALVVSLHDSSEQVF
                     TGAHEVFLGSQDLSSRTDEQVAALQQTASSMEQITALVQQNSESTEQANRLAAAAART
                     AESGGKDVEQSIQLMQELATSSQKINDIITVIDSIAFQTNILALNASVEAARAGEQGR
                     GFAVVAAEVRMLASRSAASANEIRALIEETGSKINHGVRQAEHSGQTIRETVASIGQL
                     STLMQEIANATREQSAGIGQINTAINQLDSTTQQNATLVEQSRAAVATLENQAGQMKQ
                     LVSTFKTNAPAYREERQIDNAARSVFPSASRYTEAEPRDAMWAAC"
     gene            complement(3216..3668)
                     /locus_tag="VIO83_00450"
     CDS             complement(3216..3668)
                     /locus_tag="VIO83_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HEY8329792.1"
                     /translation="MSLAKHLARQSASVARFIQLLEAEAQQLSAGRVDGQRLSTLASD
                     KQALLDEIEQLETQRQYAQRRLGYGEDRAGATRAAEDAGCLATWQQLLEQAVRAQQLN
                     HLNGASIRNRLEHNQRMLNFLHEAAGKGLYGPDGQARRGSLGGIDSRA"
     gene            complement(3691..3972)
                     /gene="flgM"
                     /locus_tag="VIO83_00455"
     CDS             complement(3691..3972)
                     /gene="flgM"
                     /locus_tag="VIO83_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HEY8329793.1"
                     /translation="MKIDSSHSPTRPTAAEGKEASARTLRSQSKPLAESPSTVSHLSA
                     AGGKNATQDVDTARVAEIRQAILEGRLQISAERIADGLIASVREQLEQD"
     gene            complement(4091..4798)
                     /gene="flgA"
                     /locus_tag="VIO83_00460"
     CDS             complement(4091..4798)
                     /gene="flgA"
                     /locus_tag="VIO83_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEY8329794.1"
                     /translation="MPSHRRPSLARLRTLLSGVLALLALLIACHAAAEPEDLEARIHE
                     LLVGAAAGPGRQVRVAVHPGAAQLPACVNPQPFLPRGEAHMPGRVAVGVRCEGAGTHY
                     RQATVAISAEYPIARRALTAGETLTAELLELRHGDLGRLPRHAVLDMQQALGRQLTRD
                     LPQGSPLPGNALRSVPLVTRGARVRVEARASGFVISRDGTALDSGAMGKPVRIRSESG
                     EIIRARVSGRNLLSVDF"
     gene            4964..5380
                     /gene="flgB"
                     /locus_tag="VIO83_00465"
     CDS             4964..5380
                     /gene="flgB"
                     /locus_tag="VIO83_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014336499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEY8329795.1"
                     /translation="MIDKLDGALRMHQLALGLRAERQQVLANNIANADTPNFKARDFD
                     FSAELSKAAQGGQLAGGSRGLRTTDARHLTARLDSLGAEREMLYRIPAQPSMDGNTVD
                     MDFERGEFADNAVRYQASLTLMNSRIQGLKSALQPE"
     gene            5387..5794
                     /gene="flgC"
                     /locus_tag="VIO83_00470"
     CDS             5387..5794
                     /gene="flgC"
                     /locus_tag="VIO83_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014336498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEY8329796.1"
                     /translation="MSMFNIFNISSSALSAQSQRMNVTASNMANADSVTGPDGQAYRA
                     KQVIFEAQAGDSQGVGGVRVARVIEDDAPMRLEYRPGHPLADERGYVSMPNVEPVNEM
                     VNMISASRSYQANVEVMNTSKQLMLKTLTLGDS"
     gene            5806..6480
                     /gene="flgD"
                     /locus_tag="VIO83_00475"
     CDS             5806..6480
                     /gene="flgD"
                     /locus_tag="VIO83_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HEY8329797.1"
                     /translation="MTSTIDTSVINAINGGASTTASEKQKAEDLRSNFMTLLVTQLQN
                     QDPLKPMENAEMTSQLAQVNTLSGIEELNETLQGITGQIDAGQTLQASALIGRNVLVP
                     GDRVLVGSGETTPFGIELAAPVDQAKVSIHDGSGQVVRQFELGALDAGVQSFVWDGTL
                     NDGSAAPDGAYRVTVEASADEQAQQATVLSYAQVGGVSTGASGPLLDLGATLGRVGLQ
                     NIRQIL"
     gene            6547..7776
                     /gene="flgE"
                     /locus_tag="VIO83_00480"
     CDS             6547..7776
                     /gene="flgE"
                     /locus_tag="VIO83_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEY8329798.1"
                     /translation="MGFSQALSGLNAASKNLDVMGNNIANSQTIGYKSATMQFADVYA
                     GAKVGLGVSVAGVQQNFKDGNLEATGRNLDLAISGGGFLRFDQGEQIVYARNGQLTMT
                     PDGYLENAQGGRLTGYPGAAGNGGQLQQLNVPAVGLPAKATEELKATLNLDAGMAILD
                     PAAFDPLKPATYSYANGATVFDSQGTAQTLTLYFLKTGVNTWEVKAARATPEGQEFSA
                     DTTQLTFDSNGLLQNSSSINGGVAGAVSSPYESTPVTFALTGGANNLDFQLNLTGSTQ
                     FANEFELGSLNQDGFTSGSLVDISVDKKGQIIGSYSNERTQVLGTIAMANFRNPEGLS
                     PVGGAWVETSDSGQALIGEAGTGLFGGIEAGVVETSNVDLTKELVNLIIAQRNFQANA
                     QTIKLQDEVLQQAVNLR"
     gene            7789..8544
                     /locus_tag="VIO83_00485"
     CDS             7789..8544
                     /locus_tag="VIO83_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HEY8329799.1"
                     /translation="MDRILYTAMSGARASLEQQSVVSHNLANASTPGFRAQLSLMRAE
                     QVAGDGLETRTLAVASTPAADFTAGPISTTGRELDVAIRGDGWLAVQAADGSEAYTRR
                     GDLQVDANGMVSVLGHPVIGEGGALTVPLGSSVSIGNDGTLSAIGEGEAPDSISPVGR
                     LKLVSAAPGSLVRGEDGLFRQQPDATGASAALPADENVRLASGALEGSNVSPTEAMVA
                     MIDNARRYDMQMKVIGSADENAQRANSLLSLQG"
     gene            8574..9356
                     /gene="flgG"
                     /locus_tag="VIO83_00490"
     CDS             8574..9356
                     /gene="flgG"
                     /locus_tag="VIO83_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004243533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEY8329800.1"
                     /translation="MIRSLWTAKTGLESQQVQLDVIANNLANVSTNGFKRSRAIFEDL
                     LYQNLRQPGAQSSAQTNLPSGLQIGTGVRPVATERLHSQGNLQNTENSRDLAINGNGF
                     FQVLMPDDNTAYTRDGSFQIDKDGQLVTASGYPLEPSITIPENALSVTVAQDGVVSIT
                     EPGSTQSREVGQLSLATFINPPGLQSIGENLYLETDSSGPRNESTPGLNGAGRLMQGY
                     VETSNVNVVEEMVSMIQTQRAYEINSKAVETSDEMLARLTQL"
     gene            9371..10063
                     /locus_tag="VIO83_00495"
     CDS             9371..10063
                     /locus_tag="VIO83_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010567402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEY8329801.1"
                     /translation="MLSSHARIWLLAGLLPVLSLAGCAQLPRSSVVGEQEQVLIPERS
                     QTPANGAIYQARRGFQPLFEDRRPRMVGDILTVVLNEEVSASKNSASSADRAGSASLT
                     PTLVPDTRNLRLKRLGEVGFDIEGDSDFSGGGGSEANNSFTGTITVTVLDVMANGNLR
                     VRGEKQIAINQGTEFIRFSGVVNPRTITGQNTVPSTQIADARIEYVGDGYINEAQHMG
                     WLQRILINVSPF"
     gene            10086..11216
                     /locus_tag="VIO83_00500"
     CDS             10086..11216
                     /locus_tag="VIO83_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEY8329802.1"
                     /translation="MKLSVIHSLRQSLQLLGLLLGLGCAGFAQAERLRDLAEFAGVRS
                     NALVGYGLVVGLDGSGDQTMQAPFTGQSLTNMLSQLGITVPPGTNMQLRNVAAVMVTA
                     DLQAFARPGQHLDVVVSSVGNARSLRGGTLLMTPLKGVDGETYALAQGNLLVGGAGAQ
                     SGGSSVQINQQAGGRISRGAIVEREVPLQLQNERGLLELQLKQPDFGMAQRMVTAINY
                     DFGRTVASAQDGGLILLDAPRDPNGRVNFMARLESLDILPGEVAPKVIINSRSGAVVL
                     NGTVRLQNAAVAHGNLSVVIDNQPKVSQPAPFSGGETVVVPDSQITVEQQGTGLRMVA
                     PSANLLEVVKALNALGATPQDLMSILEALKAAGALRAELEII"
     gene            11216..12253
                     /gene="flgJ"
                     /locus_tag="VIO83_00505"
     CDS             11216..12253
                     /gene="flgJ"
                     /locus_tag="VIO83_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HEY8329803.1"
                     /translation="MSVADLGSQFALDLQGVQRLKQTARQDPAAGLRGASKQFEALFL
                     QMMLKSMREASPQSGLLDSQQSRFYTEMLDQQRAQQLAGHGLGLAEQLTQQLQSQAGV
                     AAAAAPPMEGALIAGIPRGTPRPLYGALQPEPDAVGRPMVESTGSSRSGPVDRGSAPA
                     ALTSAVPDGPAILVSTRGDTDSGRAHHVESFAARLQAPARAASRASGVPAELILAQAA
                     LETGWGRHEIATAKGTNSHNLFGIKAGSEWRGPTTEVRTTEYVDGQAQSRVERFRVYE
                     SFEAAFTDYARLIGNNPRYAGVVSAPDAQQAARALQRGGYATDPAYADKLIAVMATLA
                     PYDPARQLAQL"
     gene            12358..13986
                     /gene="flgK"
                     /locus_tag="VIO83_00510"
     CDS             12358..13986
                     /gene="flgK"
                     /locus_tag="VIO83_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEY8329804.1"
                     /translation="MSIFSIGVSGLHTAQTVLATTSSNISNVYTPGYNRQLPILSESF
                     VGGGVAVANIERQFNQFVSDQLNRSNGVAGALSAYHAQISQIDNLMADREAGLAPLMS
                     KFFSALEDVVASPADPASRQGVLGAAETLSGQFRAFDGYLSDMQSSLNGQIKDQVTQI
                     NNAASQVAKLNREIGMARAKTGEAPNGMLNLRDQLVAELSQRVDARLTIQDGGSYNLT
                     LSNGLSLVSGSSSFSLEAMRSSEDASRFVVGYRDPGGNLLEFADSTFSTGEIGGLMTF
                     RRETLDRSQNQLGQLAVSLAVAFNEQHAQGVNLYGDAGSAFFAIGQPVSFSNVQNTSD
                     ASLSASYDPAELGSLTASDYDVTWTQAAGYSVMRRDIGTAVAANYDEANGTLKFAGMI
                     LQVNGTPQEGDRFMVQPTRRAAGQLASNLTDAARIAAGQPEGSTGAGDNRNALKLQAL
                     QTRPVVGGQATLSQGYAAMVSDIGNRTNIAAANLSAQQGLGEQLSALQQADSGVNLDE
                     EAANLIRYQQFYQANAKVIEIGAAVFDTLLGIRA"
     gene            14006..14926
                     /gene="flgL"
                     /locus_tag="VIO83_00515"
     CDS             14006..14926
                     /gene="flgL"
                     /locus_tag="VIO83_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEY8329805.1"
                     /translation="MRISTITMYEQSLNSLNRQQGEFLKIGQHISSGRRVVNPSDDPQ
                     AAALAVGIDQAKAVDQQYANARVSARNSLSQEESVLNSTSDFIVRARTLLVQAGNGTL
                     SDVDRQAISLELQGIYEGLLGQANSADGNGRFMFGGFQDSTPPFVQGDDGKVSYVGDS
                     GVKRQQVDSARLMPSVDNGQAIFRSADDADLFATLENLIGTLATPVESETDKANLQSV
                     LATAGKELGNSFDNVLAVRAAVGARLNELDVLDVVGGNRMLNYEQVLSDRLDLDYNKA
                     ISDYTLRQVGLQAAQKAFVDVQKLSLFNQL"
     gene            complement(14927..15715)
                     /gene="fliR"
                     /locus_tag="VIO83_00520"
     CDS             complement(14927..15715)
                     /gene="fliR"
                     /locus_tag="VIO83_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017735841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEY8329806.1"
                     /translation="MVEVDFAQLQQWLAAFFWPFVRLTAFLAASPLWGHDSIPTQTKI
                     GLAVLLAVLLGPNLPPLPAVPLVSWTSLGIVIEQTLIGAAMGLTMRVILAVVQAAGEI
                     IGLQMGLGFASFFAPDTGTNTMILSRLLYMFSLLIFLALDGHLIVLEILADSFTSLPI
                     GQALDLSAGEVLVRHARIILASGLLLALPLVAALLIINLAMGILNRASPQLTVFSIGF
                     PLTMCVGIVLLMVMMNDLGRFLESLFGDGLTFLRQLVASLALAG"
     gene            complement(15717..15986)
                     /gene="fliQ"
                     /locus_tag="VIO83_00525"
     CDS             complement(15717..15986)
                     /gene="fliQ"
                     /locus_tag="VIO83_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEY8329807.1"
                     /translation="MTPESVMDLAYQGMRITLFMAGPLLLTALLVGLLISLFQAATQI
                     NEMTLSFIPKILAVFTVLVLTGPWMIKMIVDFTRDLFTSIPQMIG"
     gene            complement(16009..16713)
                     /gene="fliP"
                     /locus_tag="VIO83_00530"
     CDS             complement(16009..16713)
                     /gene="fliP"
                     /locus_tag="VIO83_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700995.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEY8329808.1"
                     /translation="MLPGLALAETGLPGIVSKPLADGSQQWSLSLQTLLLLSSMAFLP
                     AMLLMMTGFTRIIIVLGLLRTAIGTPSTPPNQVLIGLALFLTFFVMSPVLTQVHEQAW
                     QPFSRDEINFEQFIETGSRPFHAFMLGQTRETDLALFARLAKVEDLQGPEQVPMRVLL
                     PAFVTSELKTAFQIGFTIFIPFLIIDLVVASVLMALGMMMVPPATISLPFKLMLFVLV
                     DGWQLLIGSLAQSFYP"
BASE COUNT         3058 a         5294 c         5672 g         2824 t
ORIGIN      
        1 cgcgctgccc gctctggcgg cgctttgcga gtgatcattc aatggcaggc atccagcaca
       61 ttttcttcag agtttcagca gaggcttgct acatttttgc tacacgaaga acgcatgaaa
      121 tcataagcga ttgatttaga aagattatat ttaaaaatac tgccaatcca tcatcggcgc
      181 cacggagaag cgacgggacg gctcggggcg cgtggttact gggttggagc taaatttcat
      241 cagggtctca gtcgtgcgcg tttccggggt ttttcgggcg tttcagggtt tttcgggcgt
      301 ttcagggagt ttcaaggctc ggcggtaaga tttaccacct cgagaatcag gtttaccacc
      361 ggcctcgaag ggcttaccac catggtcacc atccgtaccc ggaagaacaa ggacggctcc
      421 gtgatgtaca ccgcgcagct caggatcaag cgcgatggcg tgcaagttta tcaggagagc
      481 gcaagcttca gtcgcaggaa agcggtcgag gcctgggcga aacggcgtga gacggaactg
      541 agcgagcccg gcgcgttgga gcgcgaacag tgcgggggtg gagccaccct cgtagagatg
      601 atcgagcgct atatcgagga gtcggatccg accaatccgc tcggtaagac caagaaggct
      661 actcgcgcgc cctggccgcc agctacctgg gctacctggt ttgctgggag tatctcgaca
      721 agtcccgtca gcgcctgctg gtgcgggaca tgaagagcta tggtgacaag tgggggcaac
      781 gatgtgcttt tcgatacaac gagtaagcaa aagtttctat gagaggatta gttattccca
      841 attaagcatt ttcctcatcg cctcgtgggc tgtgcctgta tccggatatc tgtacgaaac
      901 cgtccatgag gaaagcaaga tgacgatgaa ttttatggcg accctgccgg cacgcgcagc
      961 gttgggcgtg gcatccgcca gcctgctggc attttccgcc caagccacaa cctgtacgag
     1021 atccgcgacc gcaagaccga ccaggtcaag tggaccgcga cccgggcgga tctggtgttc
     1081 ggctccaact cggtgctgcg cggctacgcc gaggtctacg cccaggacga taaccaggat
     1141 aagttcgtca gggacttcgt ggccgcctgg tccaagatca tgagtgctga ccgcttcgac
     1201 ctggcctgag gccagccaca gatccccaca ttcgaagaca gggacggcga tccagtcgtg
     1261 cacctacaaa aggaagtcct ggagcggctt gatctccagg tcggcgacac ccggcacctg
     1321 ttcgaggtct ggggcggcga cggggcgtgc cctgtcctga agcaaggcag ggcgggacgg
     1381 gtgtttctgc tgatgcgtgc cgattagcac gctgcccaca tggcatcgcg gggctcggct
     1441 tcggtgtatc tggaggcgga tggaaatacg ctgcgcgcag cgttgtcgat ctgccgctcc
     1501 tcgcggtagg ccggggcatt ggtcttgaac gtcgagacca gttgcttcat ctggccagcc
     1561 tggttttcca gggtggcgac ggcggcgcgg gactgttcga ccagggtggc gttctgctgg
     1621 gtggtgctgt cgagctggtt gatcgcggta ttgatctggc cgatgccggc gctctgttcg
     1681 cgcgtggcgt tggcgatctc ctgcatcagg gtggagagct ggccgatcga ggccacggtt
     1741 tcgcggatgg tctggccgct gtgttcggcc tggcgcacgc cgtggttgat tttgctgccg
     1801 gtttcctcga tgagggcgcg tatctcgttg gccgaagcgg cgctgcggct ggccagcatg
     1861 cggacctccg cggccaccac ggcaaagccg cggccctgtt ctccggcgcg tgccgcctcg
     1921 accgacgcgt taagggcgag gatgttggtc tggaaagcga tcgagtcgat gacggtgatg
     1981 atgtcattga tcttctgcga gctggtggcg agctcctgca tcaactggat gctctgctcg
     2041 acgtccttgc caccgctctc cgcggtgcgc gccgcggcgg ccgccaggcg gttggcctgc
     2101 tcggtggatt cgctgttctg ctgcaccagc gcggtgatct gttccatgct agaggcggtc
     2161 tgctgcaggg cggccacctg ctcgtcggta cgggaagaca agtcctggct gccgaggaag
     2221 acctcgtggg caccagtgaa aacctgctca ctgctgtcgt gcagggaaac caccagcgcc
     2281 gacagcttct ggttggtttc gcccatggcc tcgagcagtt ggccggtttc gtcgtggggg
     2341 ttgccgaaga tgtcctgggt caggtcgccg tcggcgacac ggcgagtcgc ctccaatgcg
     2401 ctgtgcagcg gtgcggaaat gctgcgggtg atgcgcacgg ccatgatggc ggcgatcagc
     2461 agggccagcg cggagaatgc cagcaggccg atcagtgcgt tgtggtgttg tcgggcaatc
     2521 tgttcaccag cgccatcgag cagcttcacg gccgcctggt cgatgtcggc tgccagtttg
     2581 tccagttgct ggatctcgtc attgaactgg ccgaagacca tgtcggcaat cgacggatcg
     2641 gtaatttcgc cagcctcgat ccggccgtat acggtaccga agccgtttct gtaggcgtcc
     2701 agcaaggtgg ccgattgcag gtacagcccg cgcagttcgg catcgggcga cagcgcggct
     2761 cctgcttcga gggtgttttt cagtgcctgc tggctggcat cccagtgctg cttgtgggtc
     2821 tgcacggctt gtggattgtt gacgttgagg aagatgtcct tctcggcgcg gcgcccctca
     2881 agggacagac ggcggacttc agcggcattg ctggagaggg cgacatcgat gcggagcatt
     2941 tcgttggcgg ttcgtttctg cgcagcgagg ccctgcagtc cgacggcggt agcggcggcg
     3001 agcagcagcg cgatggcgcc aaaggcaacg ccgagacggg taccaatttt cagacgggat
     3061 agcatccaga gctccacaga caaccaagtg atggcgcatt gatatcatct ggtcgttgcg
     3121 gtttggtgac ggtgggctgc tctagggcgc gaggcgtatc gtcgagcgct tgatctggcg
     3181 gctgccggtc gttctggact gggcgctggg gggcgtcagg cccgcgagtc gatgccgccg
     3241 aggctgccgc ggcgggcctg gccatcaggg ccgtagagtc ccttgccggc ggcttcgtgg
     3301 aggaaattga gcatgcgctg gttgtgttcc aggcgattgc ggatgctcgc accgttgagg
     3361 tggttgagct gctgagcgcg cacggcctgt tcgagcagtt gctgccaggt tgccaggcag
     3421 ccggcatctt cggctgcgcg cgtggctccc gcgcggtctt cgccatagcc gagtcgccgc
     3481 tgggcatact ggcgctgggt ttccagctgt tcgatctcgt ccagcagggc ctgcttgtcg
     3541 ctcgccagtg tgctcaggcg ctgaccgtcg acccggcctg cgctcagttg ctgggcctcg
     3601 gcttcgagca gttggatgaa gcgcgccacg ctggcggatt ggcgggcgag gtgcttggcc
     3661 aggctcatgc gaactccgga gttgggttgg tcagtcttgc tcgagttgtt cgcgcacgct
     3721 ggcgatcagg ccgtcggcga tgcgctcggc gctgatctgc aggcgaccct cgaggatggc
     3781 ctggcggatc tcggccacgc gcgccgtatc gacatcctgg gtcgcgttct tgccgccggc
     3841 ggcgctcagg tggctgaccg tcgacggcga ttcggccagg ggtttgctct ggctgcgcaa
     3901 ggttctggca ctggcttcct tgccttccgc cgctgtcggg cgggtcggcg aatgagagct
     3961 gtcgattttc acgggaaatc tcccttgcat gggttctgta cgcagtatcg gccgctgacg
     4021 agccgacttg agccgttctg acggcagaag tgtgcattcc gtctcagatt atgcacgcca
     4081 tggcgatggt ctagaaatcc acgctcagca agttgcgacc gctgactcgg gcccggatga
     4141 tctcgccgga ttcgctgcgg atgcggactg gcttgcccat tgcgccactg tccagtgcgg
     4201 tgccgtcgcg gctgatgacg aagccgctgg cgcgtgcctc gacccgaacc cgtgcgccac
     4261 gggtgaccag cggcacactg cgcagggcat tgcccggtag cgggctgccc tggggtaggt
     4321 cccgggtgag ctggcggccg agagcctgtt gcatgtcgag gacggcatgg cgcggcaggc
     4381 ggccgaggtc gccgtggcgc agttccagca gctcggcagt caacgtctcg ccggcagtca
     4441 gcgcgcggcg ggcaatcgga tactcggcgc tgatcgcgac cgtcgcctgt cgatagtggg
     4501 tgccggcgcc ctcgcagcgc acgcccacgg ctacccgtcc gggcatatgc gcttcgccac
     4561 gcggcaggaa gggctggggg ttcacgcagg caggcaattg ggctgcgccc ggatgcaccg
     4621 ccaccctgac ctgccggcct gggccggcgg ccgcaccgac cagcagttcg tgtatccggg
     4681 cttccagatc ctctggctcg gcggccgcgt gacaggcgat cagaagcgcc agcagggcga
     4741 gcacgccgga cagcaacgtg cgcaaccggg cgagggaggg gcggcggtgg ctcggcatgg
     4801 cggcggctcg ggaagtgcga cgaggtggaa ttctaggcgc gcaggccatg cggcaaggct
     4861 gaaattggct ggtaaatacc ggcctgttca ggcctttgcg ttggcagggt gccccctatt
     4921 ctgtgcactg aatcactttt ccctgtgcgc ggaggtcgcc cgcatgatcg acaagcttga
     4981 cggtgcactg cgtatgcacc agctggcatt ggggctgcgt gccgagcgcc agcaggtgct
     5041 ggccaacaac attgccaacg ccgatacgcc caacttcaag gcgcgggatt tcgatttttc
     5101 cgccgagctg agcaaggctg cgcagggtgg gcagctggcc ggcggcagtc gcgggctgcg
     5161 gaccaccgat gcacgacacc tcactgcacg gctggacagc ctgggcgccg agcgcgagat
     5221 gctctatcgc atccccgcac agccgagcat ggacggcaat acggtggaca tggacttcga
     5281 gcgcggcgaa ttcgctgaca acgcggtgcg ctaccaggcc agcctgaccc tgatgaacag
     5341 tcgtatccag gggctcaagt cggccctgca gcccgagtaa tccgtcatgt cgatgttcaa
     5401 catcttcaat atttccagct ccgcactgag cgcgcagtcg cagcgcatga acgtcacggc
     5461 cagcaacatg gccaatgcgg atagcgtgac cggtcccgat ggccaggcct accgcgccaa
     5521 gcaggtgatc ttcgaggccc aggcgggcga cagccagggc gttggtggtg tgcgcgtggc
     5581 tcgggtgatc gaggacgacg cgccgatgcg actggagtac cgccccggtc atcctctggc
     5641 ggacgagcgc gggtatgtga gcatgccgaa tgtcgagccg gtcaacgaga tggtcaacat
     5701 gatctccgcc tcccgctcct accaggccaa cgtcgaggtg atgaacacca gcaagcagct
     5761 gatgctcaag accttgacgc tgggcgatag ctgaggagca ccgccatgac tagcactatc
     5821 gatacctcgg tcatcaacgc gatcaatggc ggcgcatcga cgactgccag cgagaagcag
     5881 aaggccgaag acctgcgctc caacttcatg accctgctgg tgacgcaact gcagaaccag
     5941 gatccgctca agccgatgga aaacgccgag atgacctcgc agttggctca ggtcaacacg
     6001 ctgagcggca tcgaggaact gaacgagacc ctgcagggca tcaccggaca gatcgacgcc
     6061 gggcagaccc tgcaggcctc ggcgctgatc ggacgcaatg tgctggtgcc cggcgaccgg
     6121 gtgctggtcg gcagcggaga gacgaccccg ttcggcatcg aactggcagc gccggtcgat
     6181 caggccaagg tcagcatcca tgacggcagt ggccaggtgg tgcgccagtt cgaactcggt
     6241 gcactggatg ccggcgtcca gtcgttcgtc tgggatggca cgctgaatga cggcagtgcc
     6301 gcgccggacg gcgcctaccg ggtgactgtc gaggccagtg ccgatgagca ggcccagcaa
     6361 gccacggtgc tcagctatgc gcaggtcggc ggggtcagta ccggcgccag cggcccgctg
     6421 ctggacctgg gcgccacgct ggggcgtgtc ggcctgcaga acatccgtca gatcctttaa
     6481 ccaccttttc catccggata cgcgccgcgg acctccagtc cgcgccgcca ggggagtcaa
     6541 gtcaacatgg gcttttcgca ggcactcagt ggtctcaacg cggcctccaa gaacctcgat
     6601 gtgatgggca acaacatcgc caactcgcag accatcggct acaagagcgc caccatgcag
     6661 ttcgccgatg tgtatgccgg cgccaaggtc ggtctgggtg tcagcgtggc cggggtgcag
     6721 cagaacttca aggacggcaa cctggaggcg accgggcgca acctcgatct tgccatcagc
     6781 gggggcggat tcctgcgttt tgaccaaggc gagcagatcg tctatgcgcg caacggccag
     6841 ttgaccatga ctccggacgg ctatctggag aatgcccagg gcggccgtct gaccggctat
     6901 ccgggggctg ccggcaatgg cggccagctg cagcagctga acgtcccggc cgtggggctg
     6961 ccggccaagg ccaccgagga actgaaggct accctgaacc tggatgccgg gatggcaatc
     7021 ctggatccgg cagccttcga tccgctcaag ccggccacct attcctacgc caacggtgcc
     7081 accgtcttcg attcgcaggg caccgcgcag acgctgaccc tgtatttcct caagaccggc
     7141 gtcaacacct gggaggtcaa ggcggcgcgc gcgacgcccg aagggcagga gttttccgca
     7201 gacactaccc agctgacctt cgacagcaac ggcctgctgc agaacagctc ctcgatcaac
     7261 gggggagtcg cgggtgccgt cagcagtcca tacgagtcga ctccggtcac ctttgcgctg
     7321 actggcgggg caaacaacct cgacttccag ctgaacctca ccggcagcac ccagttcgcc
     7381 aacgaattcg agctgggcag cctcaatcag gacggcttca cctccggcag cctggtggat
     7441 atttccgtcg acaagaaggg ccagatcatc ggtagctact ccaacgagcg cacccaggtg
     7501 ctgggtacca tcgcgatggc caacttccgc aatcccgagg ggctgtcgcc ggtgggcgga
     7561 gcctgggtgg aaacctccga ctccgggcag gcactgatcg gcgaggccgg cacgggcttg
     7621 ttcggcggca tcgaggccgg ggtggtggag acttccaacg tcgatctgac caaggagctg
     7681 gtcaacctga tcatcgccca gcgcaacttc caggccaacg cccagaccat caagctgcag
     7741 gacgaagtgc tgcagcaggc cgtgaacctg cgctgaggag cctgaccgat ggatcgcatc
     7801 ctctataccg ccatgagcgg cgcgcgcgcc agcctcgagc agcagtccgt ggtcagccac
     7861 aacctggcca acgcctcgac gccgggcttt cgcgcccagc tctcgctcat gcgtgccgag
     7921 caggtggctg gcgatggact ggaaacccgc acgctggcgg tagccagcac gccggctgcc
     7981 gacttcacgg cgggaccgat aagcacgacc ggtcgcgaac tggatgtcgc tatccgcggc
     8041 gatggctggc tggcggtgca ggcggccgat ggcagcgagg cctatacccg ccgcggcgac
     8101 ctgcaggtgg atgccaacgg tatggtcagt gtgctcggac acccggtgat cggcgaaggc
     8161 ggagcactga cggtaccgct gggcagctcg gtcagtatcg gcaacgacgg caccctcagc
     8221 gccatcggcg agggtgaagc accggacagc atcagtccgg tggggcgcct gaagctggta
     8281 tcggcagctc ccggcagtct ggtgcgtggc gaggacggcc tgttccgcca gcaacccgat
     8341 gccaccggcg ccagtgcagc attgccggcc gacgagaatg tgcgcctggc ctccggagct
     8401 ctggagggca gcaacgtcag cccgaccgag gcgatggtgg cgatgatcga caatgcccgc
     8461 cgctacgaca tgcagatgaa ggtcatcggc agcgccgacg aaaacgccca gcgcgccaac
     8521 agcctgctct cgctgcaggg ctgagccctg ccgaacctat tcggagaaac aagatgatca
     8581 gatcgctgtg gacggcgaaa accggcctgg agtcgcaaca ggtccagttg gacgtgattg
     8641 ccaacaacct ggccaacgtc agcaccaatg gcttcaagcg ttcgcgggca attttcgagg
     8701 acctgctgta ccagaatctg cgccagcccg gcgcccagag ttcggcgcag accaacctgc
     8761 cgtcgggcct gcagatcggc accggggtgc gtccggtggc gaccgagcgc ctgcacagcc
     8821 agggcaacct gcagaacacc gagaactcgc gggatctggc gatcaacggc aacggcttct
     8881 tccaggtgct gatgcccgac gacaacacag cctacacccg tgacggcagc ttccagatcg
     8941 acaaggacgg ccagttggtc actgccagtg gctacccgct ggaaccgtcg atcaccattc
     9001 cggaaaacgc cctgtcggtc accgttgctc aggatggcgt ggtctcgatc accgagcccg
     9061 gtagcaccca gagccgcgag gtaggccagt tgtcgctggc caccttcatc aatcccccgg
     9121 ggctgcagag catcggcgag aacctctacc tggagaccga cagctcgggc ccgcgcaatg
     9181 aaagcacgcc gggcctgaac ggggctggcc gtctcatgca ggggtatgtg gaaacctcca
     9241 acgtcaacgt ggtcgaggaa atggtcagca tgatccagac ccagcgcgcc tacgagatca
     9301 acagcaaggc ggtggagact tccgacgaaa tgcttgcccg cctgacccag ttgtaatgag
     9361 gggctcgcac atgctgtcgt cgcacgcacg catctggctg ctggccggat tgctgccggt
     9421 tctgtcgcta gccggttgtg cccagctgcc gcggtcttcg gtggtgggcg agcaggagca
     9481 ggtgctgatt cccgagcggt cgcagacgcc ggccaacggc gcgatctacc aggcccggcg
     9541 cggcttccag ccgttgttcg aggaccgccg gccgcgcatg gtcggcgata tcctcaccgt
     9601 tgtcctcaac gaggaggtca gcgccagcaa gaattcggcc tccagcgccg accgtgccgg
     9661 cagcgcgagc ctgaccccga cgctggtgcc cgatacgcgc aacctgcgtc tgaagcgcct
     9721 cggcgaggtg ggcttcgata tcgagggcga cagcgacttt tccggcggcg gcggttccga
     9781 ggcgaacaac tccttcaccg gcaccatcac cgtgacggtg ctggacgtga tggccaacgg
     9841 caacctgcgg gtacgcggcg agaagcagat cgccatcaac cagggcaccg agttcatccg
     9901 tttctccggg gtggtcaacc cccgcaccat caccgggcag aacaccgtgc cttctaccca
     9961 gatcgccgat gcccgcatcg aatatgtcgg cgacggctac atcaacgagg cccagcacat
    10021 gggctggctg caacgcatcc tcatcaatgt ctcaccgttc tgatgggcga gcgccgggaa
    10081 ctgccatgaa actctccgtt atccatagcc tgcggcagtc cctgcaactg ttggggctgc
    10141 tcctcggcct cggttgcgcg ggcttcgccc aggccgagcg cctgcgcgac ctggccgagt
    10201 tcgccggcgt gcgcagcaat gcgctggtcg gctacgggct ggtggtgggg ctggatggct
    10261 ccggcgacca gaccatgcag gcgccgttca ccggccagag cctgaccaat atgctctcgc
    10321 agctgggcat caccgtgccg ccgggcacca acatgcagct gcgcaacgtc gccgcggtga
    10381 tggtcaccgc cgatctgcag gccttcgccc ggccagggca acatctggac gtggtggtgt
    10441 cctcggtggg caacgcccgc agcctgcgtg gcggtacgct gctgatgacg cccctcaagg
    10501 gcgtggacgg cgaaacctat gcgctggccc agggcaacct gctggtcggc ggcgctggcg
    10561 ctcagtcggg tggcagcagc gtgcagatca accagcaggc cggcgggcgg atcagtcgcg
    10621 gggccatcgt cgagcgcgag gtgccgctgc agctgcaaaa cgaacgcggc ctgctcgagt
    10681 tgcagctcaa gcagccggac ttcggcatgg cccagcgcat ggtcacggcg atcaactatg
    10741 acttcggccg caccgtggcc agcgcccagg atggtgggct gatcctgctg gatgcgccgc
    10801 gcgatcccaa tggccgggtc aacttcatgg cgcggctgga gagcctggac atccttcccg
    10861 gcgaggtggc gcccaaggtg atcatcaact cgcgcagcgg cgcggtggtg ctcaacggca
    10921 cggtgaggct gcagaacgct gccgtggccc acggcaacct gtcggtggtg atcgacaacc
    10981 agcccaaggt cagccagccg gcgccgttca gcggcggcga gaccgtggtg gtgcccgatt
    11041 cgcagatcac cgtcgagcag cagggcaccg gtctgcgcat ggtcgcaccg agcgcgaacc
    11101 tgctcgaggt ggtcaaggcg ctgaatgcgc tgggtgccac gccgcaggac ctgatgtcga
    11161 ttctcgaagc actcaaggcc gccggcgcct tgcgcgcgga actggagatc atctgatgag
    11221 cgttgccgat ctgggtagtc agtttgccct cgacctgcag ggcgtgcagc gcctcaagca
    11281 gaccgcccgc caggatccgg cggcgggact gcgcggggcg agcaagcagt tcgaggcgct
    11341 gttcctgcag atgatgctga agagtatgcg cgaggcgagc ccgcagtccg gcctgctcga
    11401 cagccagcag agccgcttct acaccgagat gctcgaccag cagcgtgccc agcaactggc
    11461 cggccatggt ctgggactcg ccgagcagct gacgcagcag ctgcagagcc aggcgggcgt
    11521 cgctgcggcg gccgcgcccc cgatggaggg cgcgttgatc gccgggattc cgcgcggcac
    11581 gccgcgtccg ctgtacggcg cgctgcagcc cgagccggat gccgtcggga ggccgatggt
    11641 ggagtcgacg ggcagcagcc ggtcaggccc ggtcgatcgt ggctctgcgc cagctgcgct
    11701 gacaagcgcc gtgcccgacg ggcccgccat cctggtgagt acccgcggcg acacggacag
    11761 cgggcgggct catcacgtcg agagcttcgc cgctcgactg caggcgccag cccgcgcggc
    11821 cagccgtgcc agcggcgtgc cggccgagct gatcctggcc caggccgcgt tggaaactgg
    11881 ctggggacgc cacgagatcg ccacggccaa gggcaccaac agccacaacc tgttcggcat
    11941 caaggccggc agcgagtggc gggggccgac caccgaggtg cgcaccaccg agtatgtcga
    12001 cggccaggcg cagagccggg tcgagcgctt ccgcgtctac gagtccttcg aggcggcctt
    12061 caccgactat gcccggctga tcggcaacaa cccgcgctat gccggcgtgg tcagcgcgcc
    12121 ggatgcccag caggcggcgc gcgccctgca gcgtggcggc tatgccaccg acccggccta
    12181 tgccgacaag ctgatcgcgg tgatggcgac cctcgccccg tacgatccgg cacgccagct
    12241 ggcgcagctc tgacccggcc tcccgcggtc tgcaatgtgc ggcccgtcac gcctgcggcg
    12301 ctcaagatcg tggcggcgct gccgataacc tgagcaaggt tgaacaggaa cggcttcatg
    12361 agcatctttt ccattggcgt cagtggcctg cacaccgcgc agaccgtatt ggccaccacc
    12421 agcagcaaca tcagcaatgt ctacaccccc ggctacaatc gccagctgcc gatactgagc
    12481 gaaagcttcg tcggaggcgg tgtggcggtg gccaatatcg agcgccaatt caatcagttc
    12541 gtcagcgacc agttgaaccg cagcaatggc gtcgccgggg cgctgagcgc ctatcacgcc
    12601 cagatcagtc agatcgacaa cctgatggcc gatcgcgaag ccgggctggc accgctgatg
    12661 agcaagttct tttccgccct ggaagatgtg gtggcttcgc ccgccgatcc cgcctcccgc
    12721 cagggcgtgc tgggcgctgc agaaaccctg agcgggcagt tccgcgcctt cgacggctac
    12781 ctcagtgaca tgcagagcag cctcaacggg cagatcaagg atcaggtcac ccagatcaac
    12841 aacgccgctt cccaggttgc caagctcaac cgcgagatcg gcatggccag ggccaagacc
    12901 ggcgaggccc ccaacgggat gctcaatctg cgcgaccagt tggtcgccga gctgagtcag
    12961 cgggtcgacg cgcggctgac catccaggat ggtggcagct acaacctgac cctgagcaac
    13021 ggcttgagcc tggtcagcgg cagcagcagt ttcagtctcg aagccatgcg ttcgtccgag
    13081 gacgccagcc gcttcgtggt cggctaccgc gatccgggcg gcaacctgct ggagttcgcc
    13141 gatagcacct tcagcaccgg cgagatcggc gggctgatga cctttcgccg ggaaaccctc
    13201 gaccgatccc agaaccagct gggccagctg gcggtgtcgc tggcggtggc gttcaacgag
    13261 cagcatgccc agggtgtcaa cctctacggc gatgccggca gcgctttctt cgccatcggc
    13321 cagccggtca gtttcagcaa tgtgcagaac accagcgatg cctcgctgag tgccagctac
    13381 gaccccgcgg agctgggcag cctgaccgcc agcgattacg acgtgacctg gacccaggct
    13441 gccggttaca gcgtgatgcg tcgcgatatt ggcactgcgg tagcggccaa ctacgacgaa
    13501 gccaacggca ccctgaagtt tgccggcatg atcttgcagg tcaacggcac cccgcaggag
    13561 ggcgaccgtt tcatggtgca gcccacccgc cgcgccgctg gccagttggc gagcaatctc
    13621 actgatgccg cacggattgc cgcgggccaa ccggaaggca gcacgggggc tggggacaat
    13681 cgcaatgcgc tgaagctgca ggccctgcag acccgcccgg tggtcggtgg ccaggcgacc
    13741 ctgagtcagg gctatgccgc catggtcagc gatatcggca accgtaccaa tatcgccgcc
    13801 gccaacctca gcgcccagca ggggcttggc gagcagctca gtgcgctgca gcaggccgac
    13861 tcgggggtca acctcgacga ggaagcggcc aacctgatcc gctaccagca gttctaccag
    13921 gccaacgcca aggtcatcga gatcggcgcg gcggtcttcg acaccctgct cggcattcgt
    13981 gcctgatcga taggagacaa gcgcgatgcg catcagcacc atcaccatgt acgagcagag
    14041 cctgaactcg ctgaaccgcc agcaggggga gttcctcaag attggccagc acatctccag
    14101 cggccggcgc gtggtcaatc cttcggacga cccgcaggct gccgcgctgg cggtgggcat
    14161 cgaccaggcc aaggccgtcg accagcaata cgccaatgcc cgggtcagtg cgcgcaacag
    14221 cctgtcccag gaggagagcg tgctcaacag cacctccgac ttcatcgtgc gtgcgcgaac
    14281 cctgctggtg caggccggca acgggacgct cagcgatgtc gatcgccagg ccatcagcct
    14341 ggagttgcag ggtatctacg aagggctgct cggtcaggcc aattcggcgg acggcaatgg
    14401 acgcttcatg ttcggcggct ttcaggacag cacgccgccg ttcgtgcagg gcgatgatgg
    14461 caaggtcagc tatgtcggtg acagtggcgt caagcggcag caggtcgatt cggcgcggct
    14521 gatgcccagc gtcgacaacg gccaggcgat cttcaggagt gcggatgacg ccgacctgtt
    14581 cgccaccctg gaaaacctga ttggcaccct cgcaacgccg gtggagagcg aaaccgacaa
    14641 ggcgaatctg cagtccgtgc tcgccacggc cggcaaggag ctgggcaaca gcttcgacaa
    14701 tgtcctcgcg gtgcgcgccg cggtgggcgc gcgcctcaat gagctggacg tgctcgacgt
    14761 ggtcggcggc aaccgcatgc tcaactacga gcaggtcctt tccgatcgcc tcgatctcga
    14821 ctacaacaag gcgatttccg actacaccct gcgccaggtc ggcttgcagg cggcccagaa
    14881 ggccttcgtc gacgtgcaga agctgtcgct gttcaaccag ctgtaatcag ccggccagcg
    14941 ccagactcgc caccagttgg cgcaggaagg tcaggccgtc gccgaacagg ctttccagga
    15001 agcggcccag gtcgttcatc atcaccatca gcagcacgat gcccacgcac atggtcagcg
    15061 ggaagccgat cgagaatacg gtcagctgcg gcgaggcgcg gttgaggatg cccatcgcca
    15121 ggttgatgat cagcagcgcc gccaccaagg gcagcgccag cagcaggccg gaggccagga
    15181 tgatgcgggc atggcgcacc aggacttcgc cggccgagag gtccagcgcc tgaccgatgg
    15241 gcaggctggt gaagctgtca gcgaggattt ccagcacgat caggtggccg tcgagggcga
    15301 ggaagatcag caggctgaac atgtacagca gccgcgacag gatcatggtg ttggtgccgg
    15361 tgtccggggc gaagaagctg gcgaagccca ggcccatctg caggccgatg atctcgccgg
    15421 cggcctgcac cacggccagg atcacgcgca tggtcaggcc catggccgcg ccgatcagcg
    15481 tctgctcgat gacgatgccc aggctggtcc aggacaccag tggcaccgcc ggcagcggcg
    15541 gcaggttcgg ccccagcagt accgccagca gcacggccag gccgatcttg gtctgggtcg
    15601 gtatgctgtc gtggccccac agcggcgagg cggcaagaaa ggcggtgagg cgcacgaacg
    15661 gccagaagaa ggcggccagc cactgctgca gctgggcgaa gtcgacctcg accatgctag
    15721 ccgatcatct gcgggatact ggtgaacagg tcgcgggtga aatcgacgat catcttgatc
    15781 atccacggcc cggtcaggac cagcacggtg aacaccgcga ggatcttcgg aatgaacgac
    15841 agggtcattt cgttgatctg cgtggcggcc tggaacaggc tgatcagcag gccgaccagc
    15901 agcgcggtga gcagcagcgg gccggccatg aacagggtga tgcgcatgcc ctggtaggcg
    15961 aggtccatga ctgattcggg ggtcatagca ctgctccggt ggcttccgtc atgggtagaa
    16021 actctgcgcc agcgaaccga tcaacagctg ccagccatcc accagcacga acagcatcaa
    16081 cttgaacggc agcgagatgg tcgccggtgg caccatcatc atgcccagcg ccatcagcac
    16141 gctggccacc accaggtcga tgatcaggaa cgggatgaag atggtgaaac cgatctggaa
    16201 cgcggtcttc agctcgctgg tgacgaaggc cggcagcagc acgcgcatcg gcacctgctc
    16261 cggaccctgg aggtcttcga ccttggccag gcgcgcgaac agcgccaggt cggtctcgcg
    16321 ggtctggccg agcatgaagg catggaacgg ccggctgccg gtctcgatga actgctcgaa
    16381 gttgatctcg tcgcgggaga acggctgcca ggcttgttcg tgcacctggg tcagcaccgg
    16441 cgacatgacg aagaaggtga ggaacagcgc caggccgatc agcacctgat tgggcggcgt
    16501 cgagggcgtg ccaatggcgg tgcgcagcag gccgaggaca atgatgatgc gggtgaagcc
    16561 ggtcatcatc agcagcatgg ccggcaggaa ggccatcgag ctgagcagca gcagggtctg
    16621 caggctcagc gaccattgct ggctgccgtc ggccagcggc ttgctgacga tgccgggcag
    16681 gccggtttcg gccagggcga ggcctggcag catgagcagg gtcagcacgg ccagcagcca
    16741 agcggggcag ggcaggcgct tcatggctgg cgggacgctc cgcggcgcag gttgtgcttg
    16801 agcgcctggg ccaggcgctc ggcgaagcgg ccgtctggcg cggcggtc
//
LOCUS       DATCUN010000016         6618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_19099, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000016 DATCUN010000000
VERSION     DATCUN010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19099"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VIO83_00535"
     CDS             complement(<1..586)
                     /locus_tag="VIO83_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY8329809.1"
                     /translation="MSGLRDAWQHAPTQRRVWALAGPMILSNLSVPLVALVDSAVVGH
                     LPHAHQLGAVAVGGSLYSLLVWTFGFLRMGTTGFAAQACGRSDGGALRQVLLQGLLLA
                     VGLALLGSLLVIPLLPTLLALMQPGAALDALAREYLHIRLFGLPAALASYALIGWLLG
                     TQNARAPLLMLLTANLLNVALDLWFVLGLDWGVAG"
     gene            complement(583..876)
                     /locus_tag="VIO83_00540"
     CDS             complement(583..876)
                     /locus_tag="VIO83_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329810.1"
                     /translation="MQVESFFEWLGEALGTLIRVLVESLSGLFGLLSSAGRHFLDGLS
                     HALGMQPSLLGFLTLVIGLLLLAAAVRAFIRRAFVRGTIWLLLGLWLLSWLIQ"
     gene            complement(921..1373)
                     /locus_tag="VIO83_00545"
     CDS             complement(921..1373)
                     /locus_tag="VIO83_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016703794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8329811.1"
                     /translation="MSLPSWFLLALPLLSGAMMPLQAGVNGQLARHIDSVLSASLISF
                     LVGSLALLFITLAQRELPTGLAAFRGLSWWHWTGGLLGVLFVVSAAFAGPRIGALLFM
                     SLVLAGQLSAALLLDHFGWAGFPQASVTAGKIAGLLLIVAGVWLIRRG"
     gene            1535..4606
                     /gene="fdnG"
                     /locus_tag="VIO83_00550"
     CDS             1535..4606
                     /gene="fdnG"
                     /locus_tag="VIO83_00550"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016497753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2123..2125,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HEY8329812.1"
                     /translation="MDMNRRQFFKVCGVGLGASSLAALGMAPPPAYAETIRHFKLAST
                     RETRNTCPYCSVGCGLIMYSQGDGGKNVAQEIIHIEGDADHPVNRGTLCPKGAGLLDF
                     VHSPNRLKYPEVREAGSNQWQRVSWDEALERIARLMKTDRDANFVEKNAAGQTVNRWL
                     STGFLAASASSNEAGYITHKVVRSLGMLAFDNQARVUHGPTVAGLAPTFGRGAMTNHW
                     SDIKNADLVLIMGGNAAEAHPCGFKWVTEAKAHNKARLIVVDPRFNRSASVADLYAPI
                     RTGTDIAFLGGLINYLIANDKIQHEYVRNYTDVSFVVKEGFGFEDGLFNGYDEAKRSY
                     PDKSAWGYEIGADGYAVVDPTLSHPRCVFNLLKQHYSRYTPEVVSSICGTPQETMLKV
                     WAAIAETSQPGKVMTIMYALGWTQHTVGSQMIRTGAMVQLLLGNIGMPGGGMNALRGH
                     SNIQGLTDLGLLSNLLPGYLTLPSDGEQDYAAYIAKRTSLPLRPGQLSYWQNYEKFHV
                     SLMKAWFGKGASAENNWAYDWLPKLDVPGYDVLKVFDMMFQGQINGYFCQGFNPIAAF
                     PNKAKVGAALARLKYLVIMDPLATDTSEFWRNAGEFNDVDTASIQTTVFRLPTSCFAE
                     EDGSLVNSGRWLQWHWKAAEPPGEARTDIAIMAALFHRLRALYTTEGGAFPEPILNLD
                     WPYLRPDEPGPDELAREFSGKALADLSDPATGAVLARAGEQLAGFAQLRADGSTASGC
                     WIFSGSWTQAGNQMARRDNADPYGMGQTLGWAWAWPANRRILYNRASADPSGRPWDPV
                     KKRLVWWNGKAWGGTDVPDFKADSPPEAGMNPFIMNPEGVARLFAVDKMAEGPFPEHY
                     EPFETPIGRNPLHGNAQAISNPAARVFKNDLEVFGKAEEFPYAATTYRLTEHFHYWTK
                     HCRLAAITQPEQFVEIGETLAKDLGIKAGDRVKVSSNRGYIKAVAVVTKRLRPLTVDG
                     KTVHHVGIPIHWGFTGVAKNGFITNTLTPFVGDGNTQTPEFKSFLVNVEKV"
     gene            4608..5534
                     /gene="fdxH"
                     /locus_tag="VIO83_00555"
     CDS             4608..5534
                     /gene="fdxH"
                     /locus_tag="VIO83_00555"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HEY8329813.1"
                     /translation="MASQDIIARSATTTPMPQVRQVGEVAKLIDTSKCIGCKACQVAC
                     SEWNELRDEVGTNAGVYDNPNDLSADSWTLMRFAEYENPGTGNLEWLIRKDGCMHCAE
                     PGCLAACPSPGAIVKYANGIVDFNQDKCIGCGYCITGCPFDVPRISQKDHKAYKCTLC
                     SDRVAVGLEPACVKTCPTGAIVFGSKEDMQEHAAGRVKDLKERGFDQAGLYDPDGVGG
                     THVMYVLHHADQPSLYAGLPDAPAISPMVSLWKGLTKPLALLAMGATVLAGFFHYVRV
                     GPNRVDEEEDPGEPTVHQVDPTVHVVDPHKQP"
     gene            5642..6265
                     /locus_tag="VIO83_00560"
     CDS             5642..6265
                     /locus_tag="VIO83_00560"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEY8329814.1"
                     /translation="MKHEIQRYTPSERTNHWAVAILFFLAGLSGLALFHPALFWLSGL
                     FGGGVWTRILHPFLGLAMFALFLIMALRFAGHNRLNANDRQWLGQMGDVLNNREERLP
                     EVGRYNAGQKVLFWVLVVCLLLLLVSGIVIWREYFGQLFGVGLIRLAVLVHAVAAFVL
                     IVSIIVHIYAAIWVKGSVDAMLTGKVSRAWARRHHPGWYKQVERQRK"
BASE COUNT         1203 a         2287 c         2211 g          917 t
ORIGIN      
        1 caccagccac gccccagtcc agaccgagga cgaaccacag gtcgagcgcc acgttgagca
       61 ggttggcagt cagcagcatg agcagcgggg cgcgagcatt ctgcgtgccg agcagccagc
      121 cgatcagcgc atagctggcc agcgccgccg gcaggccgaa cagacggatg tgcaggtact
      181 cgcgggccag ggcgtccagc gcggcacccg gctgcatcag cgccagcagc gtcggcagca
      241 gcgggatgac cagcaggctg cccagcagcg ccaggccgac ggccagcagc aggccctgta
      301 gcagtacctg gcgcaacgca ccaccgtcgc tgcgaccgca ggcctgggcg gcgaagccgg
      361 tggtgcccat gcgcaggaaa ccgaaggtcc acaccagcag gctgtacagg ctgccgccaa
      421 ccgccaccgc gccgagctgg tgggcgtgcg gcaggtggcc gaccaccgcg ctgtcgacca
      481 gcgccaccag cggcaccgac aggttggaga ggatcatcgg gccagccagc gcccacaccc
      541 gacgctgggt gggggcgtgc tgccaggcgt cgcgcaggcc gctcactgga tcagccagct
      601 cagcagccag aggccgagca gcagccagat tgtcccgcgg acgaaggcgc ggcggatgaa
      661 ggcacgtacg gcggcggcca gcagcaacag gccgatcacc agggtcagga agccgagcag
      721 cgagggctgc atgcccaagg cgtgcgacag cccgtcgaga aaatgccggc cagcgctgga
      781 gagcaggccg aatagcccgc tcaggctctc gacgaggacg cggatcagcg tgcccaaggc
      841 ctcgcccagc cattcgaaga agctttcaac ctgcatgtgg tcctctgcaa aagggggacg
      901 gggggctcgg cgaggcggga tcagccgcgg cggatcagcc acaccccggc gacgatcaac
      961 agcagtccgg cgatcttgcc tgcggtcacc gaggcctggg gaaagccggc ccagccgaaa
     1021 tggtcgagca gcagcgcggc gctcagctgg ccggccagca ccagcgacat gaacagcagg
     1081 gcaccgatgc gcgggccggc gaaggcggcg ctgaccacga acagcacgcc gagcaagccg
     1141 ccggtccagt gccaccagct caggccgcgg aaggcggcga ggccagtggg cagttcgcgc
     1201 tgggccaggg tgatgaacag cagcgccagg ctgccgacca ggaaggagat cagcgaggcg
     1261 ctcagcacgc tgtcgatgtg ccgcgccagc tggccgttga cgccggcctg cagcggcatc
     1321 atggcgccgg aaagcagggg cagggcgagc aggaaccagc tgggcaagga catggacgac
     1381 tccggtcggg ggcaggcgac gcggcatagt atgccgcaat cgctcgcccc accggcggcg
     1441 atgactggca gcggcggccg gctggactat ggttaaggcg atgcggtcgc ccggtcggcc
     1501 cgggcggcct tttctttccc gagaggagag cacgatggac atgaaccgtc ggcagttctt
     1561 caaggtctgc ggtgtgggcc ttggggcgtc gagcctggcg gcgctgggca tggcgccccc
     1621 gccggcctac gccgagacga tccgccactt caagctggcc agcacccgcg agacgcgcaa
     1681 cacctgcccg tactgctcgg tcggctgcgg cctgatcatg tacagccagg gcgacggcgg
     1741 caagaacgtc gcccaggaaa tcatccacat cgagggcgat gccgaccacc cggtcaaccg
     1801 cggtaccctg tgcccgaagg gcgcgggcct gctcgacttc gtgcacagcc ccaatcgcct
     1861 caagtacccg gaagtgcgcg aggccggcag caaccagtgg cagcgggtga gctgggacga
     1921 ggcgctggag cgcatcgcca ggctgatgaa gaccgaccgc gacgccaact tcgtcgagaa
     1981 gaacgccgcc gggcagacgg tcaaccgctg gctgagcacg ggcttcctgg ccgcctcggc
     2041 gtcctccaac gaagcgggct acatcaccca caaggtggtg cgttcgctgg gcatgctggc
     2101 gttcgacaac caggcgcgtg tctgacacgg cccgacggtg gcaggtcttg ccccgacgtt
     2161 tggccgtggc gccatgacca atcactggtc cgacatcaag aatgcggacc tggtgctgat
     2221 catgggcggc aacgccgccg aagcccaccc ctgcggcttc aagtgggtga ccgaagcgaa
     2281 ggcgcacaac aaggcgcgcc tgatcgtggt cgacccgcgc ttcaaccgtt ccgcctcggt
     2341 ggccgacctg tacgcgccga tccgtaccgg caccgacatc gccttcctcg gcgggctgat
     2401 caactacctg atcgccaacg acaagatcca gcacgagtac gtgcgcaact acaccgatgt
     2461 gtcgttcgtg gtgaaggaag gcttcggctt cgaggacggc ctgttcaacg gctacgacga
     2521 ggccaagcgc agctatccgg acaagtccgc ctggggctac gagatcggcg cggacggcta
     2581 tgcggtggtc gacccgaccc tcagccaccc gcgctgcgtg ttcaacctgc tcaagcagca
     2641 ctacagccgc tatacgccgg aagtggtcag cagcatctgc ggcacgccgc aggagaccat
     2701 gctcaaggtc tgggcagcca tcgccgagac ctcgcagccg ggcaaggtga tgaccatcat
     2761 gtacgccctc ggctggaccc agcacaccgt gggctcgcag atgatccgca ccggcgccat
     2821 ggtgcagctg ctgctcggca acatcggcat gcccggcggc gggatgaacg ccctgcgcgg
     2881 ccactccaac atccagggcc tgaccgatct gggtctcttg tccaacctgc tgccgggcta
     2941 cctgaccctg ccgagcgacg gcgagcagga ctacgccgcc tacatcgcca agcgcaccag
     3001 cctgccgctg cgtccggggc agctgtccta ctggcaaaac tacgagaagt tccacgtcag
     3061 cctgatgaag gcctggttcg gcaagggcgc cagcgcggag aacaactggg cctacgactg
     3121 gctgcccaag ctggacgtgc ccggctacga cgtgctcaag gtgttcgaca tgatgttcca
     3181 ggggcagatc aacggctact tctgccaagg cttcaacccc atcgctgcct tccccaacaa
     3241 ggccaaggtc ggcgcggcgc tggccaggct caagtacctg gtgatcatgg acccgctggc
     3301 caccgacacc tcggagttct ggcgcaacgc cggcgagttc aacgacgtcg acaccgccag
     3361 catccagacc acggtgttcc gcctgccgac ctcctgcttc gccgaggagg atggctcgct
     3421 ggtcaacagc gggcgctggc tgcagtggca ctggaaggcc gcagagccgc ccggcgaggc
     3481 gcgcaccgac atcgccatca tggccgcgct gttccatcgc ctgcgcgcgc tctacaccac
     3541 ggagggcggc gccttccccg agccgatcct caacctcgac tggccctatc tgcgcccgga
     3601 cgagccgggg ccggacgagc tggcccgcga gttcagcggc aaggcactgg ccgacctcag
     3661 cgatccggcg accggcgccg tgctggccag ggccggcgag caactggccg gcttcgccca
     3721 gctgcgcgcc gatggctcca ccgccagcgg ctgctggatc ttcagcggca gctggacgca
     3781 ggccggcaac cagatggcgc ggcgcgacaa cgccgacccc tatggcatgg gccagaccct
     3841 cggctgggcc tgggcctggc cggccaaccg gcgcatcctc tacaaccgcg cctcggccga
     3901 cccctcgggc cggccgtggg acccggtgaa gaagcgtctg gtgtggtgga acggcaaggc
     3961 ctggggcggc accgacgtgc cggacttcaa ggccgactcg ccgccggaag ccgggatgaa
     4021 cccgttcatc atgaaccccg aaggggtggc gcgcctgttc gccgtcgaca agatggccga
     4081 gggcccgttc cccgagcact acgagccgtt cgagacgccg atcgggcgca acccgctgca
     4141 cggcaacgcc caggcgatca gcaacccggc ggcacgggtg ttcaagaacg acctggaagt
     4201 gttcggcaag gcggaggagt tcccctatgc ggcgaccacc taccggctga ccgagcactt
     4261 ccactactgg accaagcact gccggctcgc cgccatcacc cagcccgagc agttcgtcga
     4321 gatcggcgaa acgctggcca aggatctcgg catcaaggcc ggcgaccggg tcaaggtcag
     4381 ctccaaccgc ggctacatca aggcggtggc ggtggtgacc aagcgcctgc gaccgctgac
     4441 ggtggacggc aagaccgtgc accacgtcgg catcccgatc cactggggct tcaccggggt
     4501 ggcgaagaac ggcttcatca ccaacaccct gacccccttc gtcggcgacg gcaatacgca
     4561 gaccccggag ttcaagtcgt tcctcgtcaa cgtggagaag gtgtgacatg gccagccagg
     4621 acatcatcgc ccgttccgcc accaccacgc cgatgccgca ggtacgccag gtcggcgagg
     4681 tggccaagct gatcgacacc tccaagtgca tcggctgcaa ggcctgccag gtcgcctgct
     4741 cggagtggaa cgagctgcgc gacgaggtcg gcactaacgc cggcgtctac gacaacccca
     4801 acgacctcag cgccgactcc tggacgctga tgcgcttcgc cgagtacgag aaccccggga
     4861 cgggcaacct cgaatggctg atccgcaagg acggctgcat gcactgcgcc gagcccggct
     4921 gcctggcggc ctgtccgagc ccgggagcca tcgtcaagta cgccaacggc atcgtcgact
     4981 tcaaccagga caagtgcatc ggctgcggct actgcatcac cggctgcccg ttcgacgtgc
     5041 cgcgcatctc gcagaaggac cacaaggcct acaagtgcac cctgtgctcc gaccgcgtgg
     5101 cggtgggcct ggagccggcc tgcgtgaaga cctgcccgac cggcgccatc gtgttcggca
     5161 gcaaggagga catgcaggag catgccgccg ggcgggtcaa agacctcaag gagcgcggct
     5221 tcgaccaggc cgggctgtac gacccggacg gcgtcggcgg cacccacgtg atgtatgtgc
     5281 tgcaccatgc cgaccagccg tcgctgtatg cggggctgcc cgatgcgccg gcgatcagcc
     5341 ccatggtcag cctgtggaag ggcctcacca agccgctggc gctgctggcc atgggcgcca
     5401 ccgtgctggc cggcttcttc cactacgtgc gggtcggccc gaaccgggtc gacgaggagg
     5461 aggaccccgg cgagccgacg gtgcaccagg tcgacccgac ggtgcatgtg gtggatccgc
     5521 acaagcagcc ctgagccgtt cgccgcggct cgactcaacc acgaaacacc cccctctccc
     5581 ctcagggaga gggccggggt gagggggccg gcgccacgcc gacctcttcc gggagtcaac
     5641 gatgaaacac gagatccagc gttacacccc ctcggagcgg accaaccact gggccgtggc
     5701 catcctgttc ttcctcgccg ggctgtccgg gctggcgctg ttccatccgg cgctgttctg
     5761 gctgtccggc ctgttcggcg gcggggtgtg gacgcgcatc ctgcatccgt tcctcggcct
     5821 ggcgatgttc gcgttgttcc tgatcatggc gctgcgcttc gccgggcata accgcctcaa
     5881 cgccaacgac cgccagtggc tcggccagat gggcgacgtg ctcaacaacc gcgaggagcg
     5941 cttgccggaa gtcggccgct acaacgccgg gcagaaggtg ttgttctggg tgctggtggt
     6001 ctgcctgctg ctgctgctgg tcagtggcat cgtcatctgg cgcgagtact tcggccagct
     6061 gttcggcgtc gggctgatcc gtctggccgt gctggtccat gccgtcgcgg ccttcgtgct
     6121 gatcgtcagc atcatcgtgc acatctacgc cgccatctgg gtcaagggtt cggtggacgc
     6181 gatgcttacc ggcaaggtca gccgcgcctg ggcgcgcagg catcatccgg gctggtacaa
     6241 gcaagtggag cgccagcgca aatagccgaa aggatccggc ggatggcgta acggccaggg
     6301 cgggcgcgga gcgaagcgac agtccatcgc cgtaccactg cctgccgcgc gcaagccctg
     6361 cgaatacgcg ggtcgttccc gcacaggcct gagcgaagac cgcggctcct gcgccctact
     6421 gggcgcaagg caccgccagc cagaaatttc ccccctcccg cgccatcacg ccctgatcgc
     6481 tacccgacca ccagcgcagc gcctgcgagc gtccgaattt catacggctc cggacgcggg
     6541 tatccatgca tcgaacgtcc gggggcctgc ggaattgccg tgctctcgga gcaaaatcaa
     6601 ccatttcaat tgcttgcg
//
LOCUS       DATCUN010000017         3377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_263311, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000017 DATCUN010000000
VERSION     DATCUN010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263311"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VIO83_00565"
     CDS             <1..141
                     /locus_tag="VIO83_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003116128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329815.1"
                     /translation="ASERWLPRLLGAVLLLGAASSGLALTTETWPAWSMLAGGLYLIL
                     RR"
     gene            219..623
                     /gene="hisI"
                     /locus_tag="VIO83_00570"
     CDS             219..623
                     /gene="hisI"
                     /locus_tag="VIO83_00570"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY8329816.1"
                     /translation="MSNWLDEIHWNADGLVPAIAQDHKSGRILMMAWMNRESLQLTVD
                     EGRAIYWSRSRGKLWRKGEESGHVQKLHELRLDCDADVLVLQVEQLGGIACHTGRESC
                     FYRVYENGAWQTVDAVLKDPHAIYEHKGHSHE"
     gene            616..966
                     /locus_tag="VIO83_00575"
     CDS             616..966
                     /locus_tag="VIO83_00575"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEY8329817.1"
                     /translation="MSEHSSNDTLVRLAEVLESRKGATPDSSYVASLYHKGLNKILEK
                     VGEESVETILAAKDAAVSGDCSDLIYETADLWFHSLVMLASLGQHPQAVLDELDRRFG
                     LSGHAEKAARQPSA"
     gene            990..1235
                     /locus_tag="VIO83_00580"
     CDS             990..1235
                     /locus_tag="VIO83_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY8329818.1"
                     /translation="MGIFDWKHWLVILVVVVLVFGTKRLKTLGSDVGEAIKGFRKAVN
                     TEEGDNKPAEPQAGDTLNKPQTIEGQAQKVDTPVRKD"
     gene            1245..1643
                     /gene="tatB"
                     /locus_tag="VIO83_00585"
     CDS             1245..1643
                     /gene="tatB"
                     /locus_tag="VIO83_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEY8329819.1"
                     /translation="MFDIGFSELLLVGLVALLVFGPERLPAAARTAGLWIGRLKRSFN
                     AIKTEVEREIGADEIRRQLHNEQILEMERELKQSLQPPAPAIAPISGGTPTADTPQSA
                     APDSTPAVQSPPSAQPAVAPAAPSEPPRPT"
     gene            1640..2458
                     /gene="tatC"
                     /locus_tag="VIO83_00590"
     CDS             1640..2458
                     /gene="tatC"
                     /locus_tag="VIO83_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY8329820.1"
                     /translation="MTEPSQTDGEMPLVAHLTELRSRLLRCVVAVLLIFAGLFYFAQD
                     IYTLVSAPLRAYLPEGATMIATGVASPFLTPFKLTAWVALFLSVPVILHQIWGFIAPG
                     LYKHEKRIAVPLLVSSIFLFYAGMAFVYFLVFPLMFHFFASVTPEGVAMMTDINEYLD
                     FVLTLFFAFGVAFEIPVATFLILWIGLIDVETLKKSRPYVIVGCFVVGMVLTPPDIFS
                     QTLLAVPMWLLFEVGLFFGAMVRRPQPEEGDEEASESSAVVSRDHDDQPPAPLA"
     gene            2455..3165
                     /locus_tag="VIO83_00595"
     CDS             2455..3165
                     /locus_tag="VIO83_00595"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY8329821.1"
                     /translation="MNLLLLEDGDFVAEGRALLRGRRLTHLHEVHRAEAGDSLRVGRL
                     GGLMGEGRLLRLDADAAEIEVLSLDKSPPAKLPLTLLLALPRPKMLKRVLQTVASMGV
                     PRLVLLNSYRVEKSFWQTPFLDAAAIREQLILGLEQARDTVLPEVIVEKRFKPFVEDR
                     LPQLAAGSLGLVGHPGAYPACPRAVEQAVTLAIGPEGGWIPYEVDKLHEAGLQPVQLG
                     ERILRVETAVTALLARLF"
     gene            complement(3300..>3377)
                     /locus_tag="VIO83_00600"
     CDS             complement(3300..>3377)
                     /locus_tag="VIO83_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY8329822.1"
                     /translation="ASGRFGADMQVHLVNDGPVTFLLEV"
BASE COUNT          566 a         1224 c         1061 g          526 t
ORIGIN      
        1 gccagcgagc gctggctgcc gcgcctgctc ggcgccgttc tgctgctcgg cgccgccagc
       61 agcggcctgg cgctgactac cgagacctgg ccggcctgga gcatgctcgc cggcggcctg
      121 tacctgatcc tgcgccgata gccagcgcgc aacactgctg gcaaactgag ccgattgtga
      181 acggcgccgg caaggcgccc cggacaagcg aagaaccgat gagcaactgg ctggacgaaa
      241 ttcactggaa cgccgacggc ctggtcccgg cgattgccca ggaccacaag agcgggcgca
      301 tcctgatgat ggcctggatg aaccgcgaat ccctgcagct caccgtcgac gagggccgcg
      361 ccatctactg gtcgcgctcg cgcggcaagc tgtggcgcaa gggcgaggag tccggccacg
      421 tgcagaagct ccacgaactg cgcctggact gcgacgccga cgtcctcgtc ctgcaggtcg
      481 agcagctcgg cggcatcgcc tgccacaccg gccgcgagag ctgcttctat cgcgtctatg
      541 aaaacggcgc ctggcagacc gtcgacgcgg tcctcaagga cccgcacgcc atctacgagc
      601 acaagggcca cagccatgag tgagcacagc agcaacgaca ccctcgtccg cctggccgag
      661 gtgcttgagt cccgcaaggg cgccacgccg gacagctcct acgtggccag cctgtaccac
      721 aagggcctca acaagattct ggaaaaggtc ggcgaggagt cggtggaaac catcctcgcc
      781 gccaaggacg ccgcggtcag cggcgactgc agcgacctga tctacgaaac cgccgacctg
      841 tggttccact ccctggtcat gctcgcctcc ctcggccagc atccgcaggc ggtactcgac
      901 gaactggacc gccgcttcgg cctctccggg cacgccgaga aggccgcccg tcaaccgtcc
      961 gcctaaccat tcagcacgag gagaatttca tgggtatttt cgactggaaa cactggctgg
     1021 tcattcttgt cgtcgtcgtg ctggtgttcg gcaccaagcg cctgaagacc ctcggctccg
     1081 acgtcggcga ggcgatcaag ggctttcgca aggccgtgaa caccgaggaa ggtgacaaca
     1141 agccggccga gccgcaggcc ggcgacaccc tgaacaagcc gcagaccatc gagggccagg
     1201 cccagaaggt cgacaccccg gtgcgcaagg actgagcctg acgcatgttc gatatcggtt
     1261 tttccgagct gttgctggtc ggtctggttg ccctgctggt gttcggcccc gagcgtctgc
     1321 cggcggccgc gcgcactgcc ggcctgtgga tcggccgcct gaagcgcagc ttcaacgcga
     1381 tcaagaccga ggtcgaacgc gagatcggcg ccgacgagat ccgccgccag ctgcacaacg
     1441 aacagatcct ggagatggag cgcgaactca agcagtcgct gcagccgcca gcgcccgcga
     1501 tagcgccgat cagcggcggc acgccaacgg ctgacacgcc gcagagcgcc gcccccgaca
     1561 gcactccagc cgtccaatcc cccccgtccg cccagcccgc cgtcgcgccg gctgcgccca
     1621 gtgagccgcc gcgcccgaca tgaccgagcc ctcccaaacc gacggggaaa tgcccctggt
     1681 agcgcacctc accgagctgc gcagccgcct gctgcgctgc gtggtcgcgg tgctgctgat
     1741 cttcgccggg ctgttctact tcgcccagga catctacacc ctggtctccg cgccgctgcg
     1801 cgcctacctg ccggaaggcg cgaccatgat cgccaccggg gtggcctcgc cgttcctcac
     1861 cccgttcaag ctcaccgcct gggtcgcgct gttcctcagc gtgccggtga tcctccacca
     1921 gatctggggc ttcatcgccc ccgggctgta caagcacgaa aagcgcatcg ccgtgcccct
     1981 gctggtttcc agcatcttcc tgttctacgc cgggatggcc ttcgtctact tcctggtctt
     2041 cccgctgatg ttccacttct tcgccagcgt gacgccggaa ggcgtggcga tgatgaccga
     2101 catcaacgag tacctggact tcgtcctcac cctgttcttc gccttcggcg tggccttcga
     2161 gattccggtg gcgaccttcc tgatcctgtg gatcggcctg atcgacgtgg aaaccctgaa
     2221 gaagagccgc ccctacgtga tcgtcggctg cttcgtggtc ggcatggtcc tcaccccgcc
     2281 ggacatcttc tcgcagaccc tgctggccgt gcccatgtgg ctgctgttcg aggttggcct
     2341 gttcttcggc gccatggtgc gccgcccgca gccggaagaa ggcgacgaag aggccagcga
     2401 gagcagcgcc gtagtcagcc gcgatcacga cgaccagccg ccggcacccc tggcatgaac
     2461 ctgctgttgc tggaggacgg cgacttcgtc gccgagggcc gtgcgctgtt gcgcggccgg
     2521 cgcctcaccc acctgcacga ggtgcatcgc gccgaggccg gcgacagcct gcgcgtcggc
     2581 cgcctcggcg gcctgatggg cgagggccgc ctgctgcgcc tggacgccga cgcggcggaa
     2641 atcgaggtgc tgtcgctcga caagagcccg ccggccaagc tgccactgac cctgctgctc
     2701 gccctgccgc ggccgaagat gctcaagcgc gtgctgcaga ccgtggccag catgggcgtg
     2761 ccgcggctgg tgctgctcaa cagctaccgg gtggaaaaga gtttctggca gacgccgttc
     2821 ctcgacgcgg cggcgatccg cgaacagctg atcctcggcc tcgagcaggc acgcgacacc
     2881 gtgctgccgg aggtcatcgt cgagaaacgc ttcaagccgt tcgtcgagga ccgcctgccg
     2941 caactggccg ccggcagcct cggcctggtc ggccaccctg gcgcctaccc ggcctgcccg
     3001 cgcgcggtcg agcaggcggt gaccctggcc atcggcccgg aaggcggctg gatcccctac
     3061 gaagtggaca agctgcacga ggccggcttg cagccggtac agctcggcga acgcatcctg
     3121 cgcgtggaaa ccgccgtcac cgccctgctc gcccggttgt tctgaccaaa cgtccaccgc
     3181 tcgggccgga gcggtgatgg aaaatcgctg cgcgaatttt ccaccctacg ccaaccacca
     3241 cgccgccaac tccgcagggt ggacaacggc gccgccttgt ccagcagccc ccctcgccct
     3301 cacacctcga gcagaaaggt caccggcccg tcgttgacca ggtgcacctg catgtccgcg
     3361 ccgaagcggc cgctggc
//
LOCUS       DATCUN010000018        16981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_113188, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000018 DATCUN010000000
VERSION     DATCUN010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 16981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16981
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113188"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2141
                     /locus_tag="VIO83_00605"
     CDS             <1..2141
                     /locus_tag="VIO83_00605"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8329823.1"
                     /translation="APASAQHARGGEAPLLGALFSAVSLRQLLEEGLAAEIHDSLALE
                     LHDLEDAGSTPLYRVGTPATDSPLHASRDFRSGTWHYRLTLRPTASFLLQNPSLAAWT
                     VALPGASLSLLLGILLFVLLSQRQRALALVVRRTAELQRSREALRISEERWTLALDGT
                     GDGVWDWDLLADRLYFSPMWKALLGHAEDEIGDSPEEWRSRLHPDDAERCQHALHEHL
                     AGRTPLYRCEKRLRCKDGSWLWLLARGKVVERLADGRPKRIIGTHVDISVRKALELEL
                     RQSNGRLQGLLEAATQVAIIGVDADGRIRTFNAGAERLLGYAREDIQEQPLDPLLDCP
                     RLAASAGMDVPAGGALALLEALLGSRHQHRELETILLHRNREPLPVTLMLSAIIEPDG
                     QRGGYLLLALDIGERLRARQALEERSQLLEKLGAQVPGSIYQYRLFSDGRSCFPYASA
                     GIREVYEVEPEQVRSDAAAVLERIHPQDRERIVSSIQQSARELTRWQEDYRVLLPQRG
                     LRWLRGDSMPERLADGSVLWHGFIADISSLKQVEHELRMLTITDPLTGIYNRRYFLDH
                     LGRELARHARTRRGVSLIMLDIDHFKRVNDTWGHAVGDQVLQELCRRIGARLRRLDMF
                     CRFGGEEFVIICPETEHNQALHLAEVLRHLVQDTPFARVGQVTASFGVSGVRPGDSGE
                     SLLQRADQALYAAKDSGRNRVCGEPAAISA"
     gene            2229..2708
                     /gene="coaD"
                     /locus_tag="VIO83_00610"
     CDS             2229..2708
                     /gene="coaD"
                     /locus_tag="VIO83_00610"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY8329824.1"
                     /translation="MNRALYPGTFDPITKGHGDLIERAARLFDEVIVAVAASPKKNPL
                     FSLEQRVAMVEAVTAHLPNVRVLGFSNLLAQFAQQQQANVLLRGLRAVSDFEYEFQLA
                     NMNRQLAPQVESLFLTPSEQYSYISSSLVREIAALGGDIGKFVHPAVAQALHERYPG"
     gene            2788..3039
                     /locus_tag="VIO83_00615"
     CDS             2788..3039
                     /locus_tag="VIO83_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEY8329825.1"
                     /translation="MSLIITDDCINCDVCEPECPNGAISQGEEIYEIDPNLCTECVGH
                     YDEPQCQQVCPVDCIPLDPNHVESRDELMQKYLIISGKA"
     gene            complement(3099..4922)
                     /locus_tag="VIO83_00620"
     CDS             complement(3099..4922)
                     /locus_tag="VIO83_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003282793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HEY8329826.1"
                     /translation="MWLLAVVLAVSFWFSPGWTHLAGGLALFLFGMQCLEEGLHLLAG
                     SELERFLGKATDRAWKSLGFGIFATVVVQSSSLVSLLTIAFLSTGLIGLAAGLCIIFG
                     ANLGTTSGVWLLALAGQSASISALALPLAVFGILLGFNGPRSRGVGRVMLGVCFLFLG
                     IDLMKTGFLDAGEWLDPAQIQVPGLLGAVLFAGVGMLVTALVQSSHASLMLTLTALAA
                     GQIGLEQAFALAIGANVGSTITAVLGSLGGARAGKRLAVAHVLFNGTSAVLTLLLLGP
                     LTALTRLAAAGLGLGGSVVFELALFHTLFNGLGLLLFVPWHRGLAGWLERILPEVAEP
                     RVLIAEVSNGHAPPQLPQTRARYLDENALGSAGAAVQAVARELQHLARLSLEVICHAL
                     YLPVDQLRSVQIDEALLQASAKTAGRALDAEQLYQRHIKGVYGDLLSYMGRLEVNLDE
                     AHSRAWMASQLVALQLVDAVKDAKHLQKNLSRLLTNGEPAVSAAYADLRRQLLWVLRE
                     MRAISLLDLPAEALRARLELFDNQAASFDAAFRERLFAAVREQRMDGLRAGSLMNDLG
                     YASRIGQSLRNVLLLGLGDAHEIMPQLPFRSGDEAPLIQLE"
     gene            complement(5135..5503)
                     /locus_tag="VIO83_00625"
     CDS             complement(5135..5503)
                     /locus_tag="VIO83_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEY8329827.1"
                     /translation="MNLFRSTALGLILTTGLATLPLSASAQQDNVSGDPTYTLEAPPA
                     YAMVGDLLVARPLLIAGTIIGAAVFIVSLPFSALGGNVPEAADALIGTPGREAFVRCL
                     GCTSAEKPYRQAQQDSQGQY"
     gene            complement(5616..6428)
                     /gene="mutM"
                     /locus_tag="VIO83_00630"
     CDS             complement(5616..6428)
                     /gene="mutM"
                     /locus_tag="VIO83_00630"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY8329828.1"
                     /translation="MPELPEVETTRRGIAPYLEGHRVTRVIVRERRLRWPIPEDLDVR
                     LSGQRIERVERRAKYLLIGAECGTLISHLGMSGSLRLIEAGLPAAKHEHVDIELDSGL
                     ALRYTDPRRFGALLWSTDPLEHELLRKLGPEPLGDAFDGERLYQLSRGKRVAVKPFIM
                     DNAVVVGVGNIYATEALFAAGIDPRREAGSIARARYVRLAGEIRRILAQAIDCGGTTL
                     RDFVGGDGKPGYFQQALFAYGRGGEFCKVCGGTLREVKLGQRASVYCPRCQH"
     gene            6561..7754
                     /locus_tag="VIO83_00635"
     CDS             6561..7754
                     /locus_tag="VIO83_00635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEY8329829.1"
                     /translation="MTLPSLRLKANAERRLRAGHLWVYSNEVDVAATPLSLFAAGDQA
                     ILEMSNGKPLGIVAISPQNLICARLISRDVKHMLDKSLLVHRINVALSLRERLFDKPF
                     YRLVYGDSDLLPGLVVDRFGDYLVVQMASATMEHHKHHLIEALVQVVKPASILLKNDS
                     SARDAEGLPRYVDSAYGQVPEWVALEENGVKFEAPVQGGQKTGWFYDHRMNRARLAPY
                     VKGKRVLDLFSYIGGWGVQAAAFGASEVMCVDGSALALDGVERNAALNGVADKMACVE
                     GDVFEALKELKAAEERFDVIVADPPAFIKRKKDLKNGEAAYRRLNEAAMRLLNKDGIL
                     VSASCSMHLPEDDLQNILLQSARHLDRHIQLLERGGQGPDHPVHPAIHETRYIKSLTC
                     RILPN"
     gene            7936..9774
                     /gene="ilvD"
                     /locus_tag="VIO83_00640"
     CDS             7936..9774
                     /gene="ilvD"
                     /locus_tag="VIO83_00640"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY8329830.1"
                     /translation="MPDYRSKTSTFGRNMAGARALWRATGMKDEDFKKPIIAVANSFT
                     QFVPGHVHLKDLGQLVAREIEKAGGVAKEFDTIAVDDGIAMGHDGMLYSLPSREIIAD
                     SVEYMVNAHCADAIVCISNCDKITPGMLMAALRLNIPVVFVSGGPMEAGKTKLASHGL
                     DLVDAMVAAADDSCSDEKVAEYERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGT
                     TLATHADREQLFLRAGRLIVELCQRYYGEGDESVLPRSIANRKAFENAMTLDIAMGGS
                     TNTILHLLASAQEGEVDFDLRAIDALSRRVPQLCKVAPNIQKYHMEDVHRAGGIFSIL
                     GELARGGLLHTDVPTVHSKTMADAIAQWDITQTTDEAVHTFFKAGPAGIPSQVAFSQA
                     TRWPTLDTDRFEGCIRSVENAYSQEGGLAVLYGNIAEDGCVVKTAGVDESIHVFEGTA
                     KIYESQDAAVKGILADEVKPGNIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLGKQCAL
                     LTDGRFSGGTSGLSIGHASPEAAAGGAIGLVEEGDKILIDIPNRSINLLVGAEELHAR
                     RLHQDHKGWKPAAPRARKVSTALKAYALLATSADKGAVRNKALLED"
     gene            9890..11422
                     /locus_tag="VIO83_00645"
     CDS             9890..11422
                     /locus_tag="VIO83_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329831.1"
                     /translation="MMLVARRVQRSGEARDRTEKPEELPLSDFHHTPAWVLLGEPGAG
                     KTTAFKEEARLTDGLYLTVSDLLYTSPLPDEWKGQTLFIDALDEMRASGEQSPIRRIA
                     NQLRNLRPSAFRLSCRAADWQGASDRATLEHFSPDGQLPALQLCSLNDDDIRQILQNN
                     HNVADPQTFVEQAERHGIAPLLSNPQTLKLMAEAVSGEHWPESRSEVYQLACERLAQE
                     NNRQHRNLTRAGEVACPVILKAAGQLFAVLLLSGKAGLALDRDASNGEYHTLEAFQPE
                     DFEVTARALRSGLFMPATGHDERLIPCHRSIAEYLAARWLADKLDRESLPHDRLLNLL
                     LGFDGGVVADLRGLLAWLAQHSTSIRTRLIDVDPLGIVLYGDARPFSTADRRRLLQAL
                     RQQAERFPGFRWHLWQDMTGFGALADEALLEDFRRILQTPERDQTTQSHMDCVLDIFE
                     YGHADPCLAAPLLAIIKDASFWPRLRYGALRTWLRLATPAEIKALLSQVCSNQVDDED
                     DE"
     gene            11528..13933
                     /locus_tag="VIO83_00650"
     CDS             11528..13933
                     /locus_tag="VIO83_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329832.1"
                     /translation="MFWEHWLPERAPVDQLPALLEGLCAKYETFDGDMTEWMLSRLVG
                     RLLVRTLNELGESATDAQIFNWLGSGANKRGYFNRDHESLPKIRHWLERHPERYKAVL
                     AQCVQTAGADQRHYQHLRLHDATPQADIGLWHLQQAGTLEEAAARVHLQNAVNALMWQ
                     RGAEGLSLEAIEAWGRAFPERAEWLNDWLVCDLERESWHLEDAARTRPYRERQAEQRR
                     QRTQHITPELSAISAGTASARLLEHLAMGWLNGFSDIHGETISARFANYSDLGDELML
                     AAESGFRHCLQRPDLPSAAEVIALDQAGQRHFISQPCLLGLQLNWVDAPEQVATLPDD
                     LVERLLALYFCSPHFGLDDLDGWAAQTAQQRPELFARVLVHQASAAFSAGNVSAGMVH
                     FLGREAAYAQVAQQASPELLRQFPMNAAGQQLRNLRQLLGAALLRAPECLAGLATHKL
                     ESPQLHVEQQLLWLAIQVLTGHTEGEEEFWQRLGKAEAADVAMLLTVEIVTQGIQLQA
                     LQTPQPERILGRLIESIAPHAELERSRGGVVTTAMDLGDELRGMSDWLGAQPTAEASG
                     ELRRLLACPTLAAQRYRIQSTLEQQQAHRREAEFQFLDLKAVADVLANRGPANAADLA
                     WLTLSHLDDIAHELHSDNDDGYRAFWNVWTENKTTHKNRRGENLCRDVLLTRLRSRLN
                     AHGINIAPEFDHAGDKRADLHLDYRNQLALPIEIKRDDHDKLWTALRDQLIAQYVHAP
                     KSAGHGIYLVFWFGESSRLKSPPNREPKPATPDELKTKLEAQLTEEERRRVFVRVLDV
                     SWL"
     gene            14286..14921
                     /locus_tag="VIO83_00655"
     CDS             14286..14921
                     /locus_tag="VIO83_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008042751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329833.1"
                     /translation="MLIRTIALLLAVLCSPLAWSQPPAAPAAAAASTDAALTTRLAMR
                     DLWVGHIFWVRNYALANQSGNAPQADTAAKEVVANATAIANSLQPLYGQPAADQMLKL
                     LAGHWGAIKDYSDAGVAKDAAKQKAAMAALASNAKAIAAFLAKANPYLPEDTLVTLLS
                     AHGGHHISQIDQLAKADYAGEAKTWHAMREHILVIADALAGALVKQFPEKF"
     gene            complement(14959..15474)
                     /locus_tag="VIO83_00660"
     CDS             complement(14959..15474)
                     /locus_tag="VIO83_00660"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY8329834.1"
                     /translation="MNTTPLPLALIAALADNRVIGIDNRLPWHLPADLKHFKAVTLGK
                     PIIMGRKTWDSLGRPLPGRLNLVVSRQAGLTLDGAEVFASLPAAIARADEWARERGVD
                     ELMLIGGAELYAQGLALAQRLYLTRVHLAPEGDAFFPEFAEDDWQLLSRDEQAAAAET
                     PAHAYEVWARD"
     gene            15568..>16981
                     /locus_tag="VIO83_00665"
     CDS             15568..>16981
                     /locus_tag="VIO83_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HEY8329835.1"
                     /translation="MATLLTSLNGCPATSGERKVASLFDRLLDEDYLCWFDVPLGPRG
                     QRPDFTLLHPLRGILLLEVKDWKLSTLHKLDRKQATLILPSGPKQASNPLEQVRQYCH
                     VLAGLLQRDPQLVHRDGPRQGQLLLPWGHGVILTQISRAQFDAAGLGEAMNPAQVICK
                     DELGEDQDPETLQARLWDMFTVAFPCNLGPAQVDRVRWHLFPELRLRSSQQSLFEDAP
                     EPGQPPAAAPDLIRIMDLQQEQLARNLGEGHRVIHGVAGSGKTMILGYRALHLARLLH
                     TPTLVLCFNKALAARLALQMDGHGVGEKVQVRHFHAWCGELLRRHGLALPPRGSASAY
                     NEQLVAQTLEAVAAGRIPTGQYGAILIDEGHDFAPEWFQLATQQVDPASQSLLVLYDD
                     AQSLYQRRKFSFSSVGIQARGRTTILRLNYRNTAEILQVAARFAGDGLAEQTAEEDGI
                     PRVPPQSVGRHGPQPLWRECA"
BASE COUNT         3056 a         5858 c         5443 g         2624 t
ORIGIN      
        1 gggcaccggc cagcgcgcag cacgcccgtg gcggcgaggc gccgctgctc ggtgcgctgt
       61 tcagcgccgt cagcctgcgc cagctgctgg aggaggggct ggccgcagag atccatgaca
      121 gcctggcgct ggaactccac gacctcgagg acgccggcag cacgccgctg taccgtgtag
      181 gcacgcccgc caccgacagc ccgctacacg ccagccgcga cttccgctcc ggcacctggc
      241 actaccgcct gaccctccgg cccacggcca gcttcctgct gcagaacccc agcctggccg
      301 cctggaccgt ggcgctgccg ggcgccagcc tcagcctgct gctcggcatc ctgctgttcg
      361 tcctgctcag ccagcgccag cgcgccctgg ccctggtcgt gcggcgcacc gccgaactgc
      421 aacgcagccg cgaagccctg cgcatcagcg aggagcgctg gacgctcgcc ctggacggga
      481 ccggcgacgg cgtgtgggac tgggatctgc tcgccgaccg cctgtacttc tcgccgatgt
      541 ggaaggccct gctcggccat gccgaggacg agatcggcga cagccccgag gaatggcgca
      601 gccgcctgca tccggacgat gccgaacgct gccagcacgc cctgcacgag cacctcgccg
      661 gccgcacgcc gctgtaccgc tgcgaaaagc gcctgcgctg caaggacggc agctggctgt
      721 ggctactggc ccgcggcaag gtggtcgagc gtctggccga tggccggccc aagcggatca
      781 tcgggaccca cgtcgatatc tcggtgcgca aggccctgga gctggagctg cggcagagca
      841 acgggcgcct gcagggcctg ctcgaggcgg cgacccaggt ggcgatcatc ggcgtcgacg
      901 cggacggccg aatccgcacc ttcaatgccg gtgcggagcg cctgctcggc tacgcgcgcg
      961 aggacatcca ggagcagccg ctcgacccgc tgctggactg cccgcgcctg gccgccagtg
     1021 ccggcatgga cgtgcccgcg ggcggcgccc tagcgctgct ggaagcgctg ctcggcagcc
     1081 gacaccagca ccgcgaactg gaaaccatcc tgctgcaccg caaccgcgag ccgctgccgg
     1141 tcaccctgat gctctccgcg atcatcgagc ccgatggcca gcgcggcggc tacctgctgc
     1201 tggccctcga cattggcgag cgcctgcgcg cccgccaggc cctcgaggag cgcagccagc
     1261 tgctggaaaa gctcggcgcc caggtgcccg gcagcatcta ccagtaccgc ctgttcagcg
     1321 acgggcgcag ctgctttccc tatgccagcg ccggcatccg cgaggtctac gaggtcgagc
     1381 ctgagcaggt acgcagcgat gccgcggcgg tgctcgagcg catccacccg caggaccgcg
     1441 agcgaatcgt cagttccatc cagcagtcgg cccgcgaact gacgcgctgg caggaagact
     1501 accgcgtgct gctgccgcag cgcggcctgc gctggctgcg cggcgactcc atgccggagc
     1561 gcctggccga cggctcggtg ctctggcacg gcttcatcgc cgacatcagc agcctcaagc
     1621 aggtcgagca tgagctgcgc atgctgacca tcaccgaccc gctgaccggc atctacaacc
     1681 gccgctactt tctcgaccac ctgggccgcg agctggcccg ccatgcccgg acccggcgcg
     1741 gggtcagcct gatcatgctg gacatcgacc acttcaagcg ggtcaacgac acctgggggc
     1801 acgcggtcgg cgaccaggtg ctccaggagc tgtgccggcg catcggcgca cgcctgcgcc
     1861 ggctcgacat gttctgccgt ttcggcggcg aggaattcgt catcatctgc ccggaaaccg
     1921 agcacaacca ggccctgcac ctcgccgagg tgctgcgtca tctggtgcag gacacgccct
     1981 tcgcgcgagt cggccaggtg accgccagct tcggcgtcag cggcgtgcgc cccggcgaca
     2041 gtggcgagtc cctgctgcag cgcgccgacc aggcgctgta cgccgccaag gacagtgggc
     2101 gcaaccgggt gtgcggcgag cccgcggcga tcagcgcctg agcctcgcag gccacaggtt
     2161 tggcttggcc gcccgctcgg cgctgcgcta ccatccgggc ctccccgcct cgaaagcagg
     2221 acctcaggat gaaccgagcg ctttaccccg gcactttcga tccgatcacc aagggccacg
     2281 gtgacctgat cgagcgcgct gcacggctgt tcgacgaagt gatagtcgca gtggcggcca
     2341 gcccgaagaa gaacccgctg ttcagcctgg aacagcgcgt ggcaatggtc gaggcggtga
     2401 ccgcgcacct acccaacgtg cgggtgctcg gcttttccaa cctgctcgcc cagtttgccc
     2461 agcagcagca ggccaacgtg ctgctgcgcg gcctgcgcgc ggtgtcggac ttcgagtacg
     2521 agttccagct ggccaacatg aaccgccagc tcgcgcccca ggtggaaagc ctgttcctca
     2581 ccccgtccga gcagtactcg tacatttcct cgagcctggt gcgggaaatc gccgcgctcg
     2641 gcggcgacat cggcaaattc gtccacccgg ctgtcgccca ggccctgcac gaacgctacc
     2701 cgggttgaga cacacggacg catgcaggcc gcgcgcagtt gcggcacaat aagcacgttg
     2761 accgttcctg cgtctggagt agttcccatg tcccttatca tcaccgacga ctgcatcaac
     2821 tgcgacgtct gcgagccgga gtgccccaac ggcgcgattt cccagggaga ggagatctac
     2881 gagatcgacc cgaacctgtg caccgagtgc gtcggccact acgacgagcc gcagtgccag
     2941 caggtctgcc cggtggactg cattccgctc gaccccaacc atgtggaaag ccgcgacgag
     3001 ctgatgcaga aatacctgat catcagcggc aaggcctgat caccgctggc tgagcacaac
     3061 gccggccatc ccgcgagggg tggccggcgt tgtcgtttct actccagctg gatcagcggc
     3121 gcctcgtcgc cgctgcggaa cggcagctgc ggcatgatct cgtgagcgtc gcccagcccc
     3181 agcagcagca cgttgcgcag gctctggccg atgcgactgg cgtagcccag atcgttcatc
     3241 agcgagccgg cccgcaggcc gtccatgcgc tgctcgcgca ccgcggcgaa cagccgttcg
     3301 cggaacgccg cgtcgaagct ggccgcctgg ttgtcgaaca gctccaggcg cgccctgagc
     3361 gcctcggccg gcagatccag caggctgatc gcgcgcatct cgcgcaacac ccagagcagc
     3421 tggcggcgca ggtcggcata ggccgcgctg accgccggtt cgccgttggt cagcaggcgg
     3481 ctgaggttct tctgcaggtg cttggcatcc ttcaccgcat ccaccagctg cagcgccacc
     3541 agctggctgg ccatccaggc gcggctatgc gcctcgtcca gattgacctc cagacgcccc
     3601 atgtagctca gcaggtcgcc gtacaccccc ttgatatggc gctggtacag ctgctcggca
     3661 tccagcgcgc gcccggccgt cttcgccgag gcctgcagca gcgcctcgtc gatctgcacg
     3721 ctgcgcaact ggtcgaccgg caggtacagg gcgtggcaga tcacctccag gctcagccgt
     3781 gccaggtgct gcagctcgcg ggccaccgcc tgtaccgcag cgccggccga acccagcgcg
     3841 ttctcgtcga ggtagcgggc acgggtctgc ggcagctgcg gtggcgcatg cccattgctc
     3901 acctcggcga tcagcacgcg gggctcggcc acctcgggca ggatgcgctc cagccagccc
     3961 gccaggccac ggtgccaggg cacgaacagc agcagtccca ggccattgaa cagggtgtgg
     4021 aacagcgcca gctcgaacac gacgctaccg cccaggccaa gaccggcagc cgccagccgg
     4081 gtcaaggccg tcagcgggcc gagcagcaac agggtcagca ccgcactggt gccgttgaac
     4141 agcacatggg cgacggccag gcgcttgccg gcccgcgccc cgccgagcga gccgagcacc
     4201 gccgtgatgg tgctgccgac attggcgccg atggccaggg caaaggcctg ctcgagaccg
     4261 atctgccccg ccgccagggc cgtcagggtc agcatcaggc tggcgtggct ggactgcacc
     4321 agcgcggtca ccagcatgcc gacgccggcg aacaacacgg cgccgagcag accgggcacc
     4381 tggatctgcg cggggtccag ccactcgccg gcatcgagga agccggtctt catcaggtcg
     4441 atgccgagga acaggaagca cacgccgagc atcacccggc cgacgcccct ggagcgcggg
     4501 ccattgaaac cgagcaggat gccgaacacc gctagcggca gggccagcgc gctgatgctg
     4561 gcgctctgcc cggccagggc cagcagccag acgccactgg tggtgccgag gttggcgccg
     4621 aagatgatgc acaacccggc cgccaggccg atcagcccgg tactcagaaa ggcgatggtc
     4681 agcagcgaga ccagcgagct ggactgcacc acgaccgtgg cgaagatgcc gaagcccagg
     4741 cttttccagg cccggtcggt ggccttgccg aggaagcgtt ccagctcgct gccggccagc
     4801 aggtgcagcc cttcctccag gcactgcatg ccgaatagga acagggccag cccgccggcc
     4861 agatgggtcc accccggact gaaccagaac gatacggcca gcaccacggc cagcagccac
     4921 atcacgagcg cgttagcaga atttctggac aagactcccc ccggagcagt gtgcactcgg
     4981 cacgttcaag caatggccgt gcgcaacacc gctacgtggg cagcaatgtg gcagcagcgt
     5041 gactgccaga tggcaatcgc gtggcaattc tgcccgaaag tagcgcggga ggggggatgg
     5101 ggaaagggcg cccggacgga cgcccttcgc agactcagta ctggccttgc gaatcctgct
     5161 gcgcctgacg gtacggcttc tcggcgctgg tgcagcccag gcagcgcacg aaggcctcgc
     5221 gccccggcgt gccgatcagc gcatccgcgg cctcggggac attgccgccc agtgccgaga
     5281 acggcaggct gacgatgaac acggcggcgc cgatgatggt gccggcgatc agcagcgggc
     5341 gggcgaccag caggtcgccg accatggcat aggccggcgg ggcctccagg gtgtaggtcg
     5401 ggtcgccgct gacgttgtcc tgttgagcag acgcggacag cggaagggtg gccaggccgg
     5461 tggtcaggat caggccaaga gcggtggaac ggaacagatt catgcgcagg gtccttttgc
     5521 cgcgttgcac gtggggataa ctatatcagc gaatcgacgg ccgccagcgt gaacgccgtc
     5581 aatcgccgca gccacacctg ggtaaaagtc gcccgtcagt gctgacagcg cggacagtac
     5641 acgctggcgc gctggcccag cttgacctcg cgcagggtgc cgccacagac cttgcagaac
     5701 tcgccgccgc ggccgtaggc gaacagtgcc tgctggaagt agccgggctt gccgtcgccg
     5761 ccgacgaaat cacgcagggt ggtgccgccg cagtcgatgg cctgggcgag gatgcgcctg
     5821 atctcgccgg ccagcctgac atagcgcgcc cgcgcgatcg aaccggcctc gcggcgcgga
     5881 tcgatgccag cggcgaacag cgcctccgtg gcgtagatgt tccccacgcc gaccaccacc
     5941 gcgttgtcca tgatgaacgg cttgaccgcc acccgcttgc cgcgcgacag ctggtacagc
     6001 cgctcgccgt cgaaggcatc gcccagcggc tcgggcccca gcttgcgtag cagctcgtgc
     6061 tcgagcggat cggtgctcca cagcagcgcg ccgaagcgcc gcgggtcggt gtagcgcagc
     6121 gccagcccgg aatccagctc gatgtcgaca tgctcgtgct tggccgccgg caggccggcc
     6181 tcgatcaggc gcaggctgcc ggacatgccc agatggctga tcagggtgcc gcactcggcg
     6241 ccgatcagca ggtacttggc gcgccgctcg acccgctcga tgcgctggcc ggacaggcgc
     6301 acgtcgagat cctcggggat cggccagcgc aggcggcgct cgcggacgat cacgcgggtc
     6361 acgcgatggc cttccagata gggggcgatg ccgcggcggg tggtttcgac ttcgggcagt
     6421 tcgggcatga cggcagacac agaaaaaccg ggggcgccaa catagcagga ttgcccgtgc
     6481 ggctcacctt cgcgcgccag ccggcaggcg cccggcgccc cgccttccgc tatactcggc
     6541 gctttttgcg agagtgcccc atgaccctgc ccagcctgcg actgaaagcc aacgccgagc
     6601 gccgcctgcg cgccggccac ctgtgggtct acagcaacga ggtggacgtg gcggctaccc
     6661 cgctcagcct gttcgccgcc ggcgaccagg ccatcctcga gatgagcaac ggcaagccgc
     6721 tgggcatcgt cgcgatcagc ccgcagaacc tgatctgcgc gcggctgatc tcccgcgacg
     6781 tcaagcacat gctcgacaag tcgctgctgg tgcaccgcat caacgtcgcc ctcagcctgc
     6841 gcgagcgcct gttcgacaag ccgttctacc gcctggtcta cggcgactcc gacctgctgc
     6901 ccggcctggt ggtcgaccgt ttcggcgact acctggtggt gcagatggcc tcggcgacca
     6961 tggagcacca caagcaccac ctgatcgagg cgctggtgca ggtggtcaag ccggccagca
     7021 tcctgctcaa gaacgattcc tccgcccgcg atgccgaggg cctgccgcgc tacgtcgaca
     7081 gcgcctacgg ccaggtgccg gagtgggtcg cgctggaaga gaacggcgtc aagttcgagg
     7141 ccccggtgca gggcggccag aagaccggct ggttctacga ccaccgcatg aaccgtgcgc
     7201 gcctggcgcc ctacgtcaag ggcaagcgcg tgctcgacct gttcagctac atcggcggct
     7261 ggggcgtgca ggccgcggcg ttcggcgcct ccgaggtgat gtgcgtggac ggttcggcgc
     7321 tggcgctgga cggcgtcgag cgcaacgccg cgctgaacgg cgtcgccgac aagatggcct
     7381 gcgtcgaggg tgacgtgttc gaggcgctca aggagctgaa ggccgccgag gagcgcttcg
     7441 acgtgatcgt cgccgacccg cccgccttca tcaagcgcaa gaaggacctg aagaacggcg
     7501 aggccgccta ccgccgcctc aacgaggccg ccatgcgcct gctcaacaag gacggcatcc
     7561 tggtcagcgc cagctgctcc atgcacctgc cggaagacga cctgcagaac attctcctgc
     7621 agagcgcccg tcacctcgac cgccacatcc agctgctcga gcgcggcggc cagggcccgg
     7681 accatccggt acacccggcg atccacgaga cccgctacat caagagcctg acctgccgca
     7741 tcctgcccaa ctgaggcggg cagaccgtag ggtggaaaac tcgcgcagcg attttccacc
     7801 ttgcctttgc gatggtggca aaggcctgcg gccgttttcc accctaccgt cggccccgcc
     7861 gtccagcctg tgcgcccgcc gccaatcgcg ttatcctgaa aacttccgta tctgcctgca
     7921 ttccgggaac ccgccatgcc tgactatcgc tcgaaaacct ccaccttcgg ccgcaacatg
     7981 gccggcgccc gcgcgctgtg gcgcgccacc gggatgaagg acgaggactt caagaagccg
     8041 atcatcgccg tcgccaactc cttcacccag ttcgtgcccg gccacgtgca cctgaaggac
     8101 ctcggccagc tggtcgcccg cgagatcgag aaggccggcg gcgtggccaa ggaattcgac
     8161 accatcgccg tcgacgacgg catcgccatg ggccacgacg gcatgctcta ttcgctgccg
     8221 agccgcgaga tcatcgccga ctccgtcgag tacatggtca acgcccactg cgccgacgcc
     8281 atcgtctgca tctccaactg cgacaagatc acccccggca tgctgatggc cgccctgcgc
     8341 ctgaacatcc cggtggtgtt cgtctccggc gggccgatgg aagccggcaa gaccaagctg
     8401 gccagccacg gtctcgacct ggtcgacgcc atggtcgccg ccgccgacga cagctgctcg
     8461 gacgagaagg tcgccgaata cgagcgcagc gcctgcccga cctgcggcag ctgctccggc
     8521 atgttcaccg ccaactcgat gaactgcctg accgaggccc tcggcctgtc cctgccgggc
     8581 aacggcacca ccctggccac ccacgccgac cgcgagcagc tgttcctgcg cgccggccgg
     8641 ctgatcgtcg agctgtgcca gcgctactac ggcgagggcg acgagtcggt gctgccgcgc
     8701 agcatcgcca accgcaaggc gttcgagaac gcgatgaccc tcgacatcgc catgggcggc
     8761 tcgaccaaca ccatcctgca cctgctggcc tccgcccagg aaggcgaggt cgacttcgac
     8821 ctgcgcgcca tcgacgcgct gtcgcgtcgc gtgccgcagc tgtgcaaggt ggcaccgaac
     8881 atccagaagt accacatgga agacgtgcac cgcgccggcg gcatcttctc gatcctcggc
     8941 gagctggccc gcggcggcct gctgcacacc gacgtgccga ccgtgcacag caagaccatg
     9001 gccgacgcca tcgcccagtg ggacatcacc cagaccaccg acgaggccgt gcacaccttc
     9061 ttcaaggccg gcccggcggg catccccagc caggtggcgt tcagccaggc gacccgctgg
     9121 ccgactctcg acaccgaccg cttcgagggc tgcatccgca gcgtcgagaa cgcctactcc
     9181 caggaaggcg gcctggccgt gctgtacggc aacatcgccg aggacggctg cgtggtgaag
     9241 accgccggcg tcgacgagtc gatccacgtg ttcgaaggca cggcgaagat ctacgagagc
     9301 caggacgccg cggtgaaggg catcctcgcc gacgaggtga agccgggcaa catcgtgatc
     9361 atccgctacg aaggcccgaa gggcggcccg ggcatgcagg aaatgctcta cccgacctcg
     9421 tacctgaagt ccaagggcct gggcaagcag tgcgccctgc tcaccgacgg tcgcttctcc
     9481 ggcggcacct ccggcctgtc gatcggccac gcctcgccgg aagcggccgc gggcggtgcc
     9541 atcggcctgg tcgaggaagg cgacaagatc ctcatcgaca tccccaaccg cagcatcaac
     9601 ctgctggtcg gcgccgagga actgcacgcc cgccgcctgc accaggacca caagggctgg
     9661 aagccggccg cgccgcgcgc gcgcaaggtc agcaccgcgc tgaaggccta cgccctgctc
     9721 gccaccagcg ccgacaaggg cgcggtgcgc aacaaggcgc tgctcgagga ctgattgcag
     9781 cccgcacggc gatcacgtaa agcccggcca tgagccgggc tttacgcttt ctggggtgtc
     9841 gtcagccgct gtatcctgcc ccgcacgcag gttcagtcgg gagcaatgga tgatgctggt
     9901 agccaggcgc gtgcagaggt cgggggaagc gcgagacagg acggaaaagc cggaagagct
     9961 gccgcttagc gactttcacc acacgcctgc ctgggtgctg ctgggcgagc cgggggcagg
    10021 caagacgacc gcgttcaagg aagaggcgcg cttgaccgat gggctttatc tcactgtcag
    10081 cgatctgctc tataccagcc cgttacccga tgaatggaaa ggacagacgc tgttcatcga
    10141 cgcgctggat gagatgcgtg catcgggtga gcaaagccct attcgacgca tcgccaacca
    10201 gctgcgcaat ctgcgcccct cagcgtttcg tctctcctgc cgggctgccg actggcaggg
    10261 cgcatcagat cgggcgacgc tggagcattt cagcccggat ggccagctgc ccgccttgca
    10321 gctgtgctca ctgaatgacg atgacattcg acagatactg caaaacaatc acaacgtcgc
    10381 cgatccccag accttcgtcg aacaggccga gcggcatgga atagccccgc tgctgagcaa
    10441 cccgcagacg ctcaagctga tggccgaggc cgtcagcggt gagcactggc cagaaagccg
    10501 cagtgaggta taccagctcg cctgtgaaag gctggcgcag gaaaacaacc ggcagcaccg
    10561 taacctgacc cgtgctggcg aagttgcctg cccagtcatc ctgaaggctg ccgggcagct
    10621 gtttgctgtt ttgctgctgt ctggcaaagc cggtcttgcc cttgatcggg atgccagcaa
    10681 tggcgaatac cacacgctgg aggcgttcca gcctgaagat ttcgaggtca ccgcgagagc
    10741 cctgcgctcc gggctgttca tgccggcgac aggccatgat gagcgcctga ttccctgtca
    10801 ccgcagcatc gccgagtatc tggcagccag atggctagca gacaagctgg atcgggaaag
    10861 cctgccacac gaccgactgc tcaatctgct gcttggcttc gacggtggcg tggtggccga
    10921 cttgcgtggt ctgctcgcct ggctggccca gcacagtacc tcgatacgca cgcggctgat
    10981 cgacgtcgat ccgcttggta tcgtgctgta cggcgatgcc cgtccattca gcacagcaga
    11041 caggcgccgt ctgctacagg ccctgcggca acaggctgag cgttttccag ggtttcgctg
    11101 gcatctctgg caggacatga caggattcgg cgcactggcc gacgaggcct tgcttgaaga
    11161 cttccggcgc atcctgcaga cgcctgagcg tgatcagact actcagtccc atatggactg
    11221 tgtgctggat attttcgagt acggtcatgc cgatccctgc ctggctgctc cgttgcttgc
    11281 aatcatcaag gatgcctctt tctggccgcg gcttcggtat ggcgctctca ggacttggct
    11341 caggctggcc acccccgcag agatcaaagc cctgctgtcg caggtttgca gtaatcaggt
    11401 tgatgacgaa gatgacgagt aactggggaa gttgctggat cggctatacc cggagtacct
    11461 gataccggat gagttattcc agtgccttca ccttcccaag gattctcacc tgatcggctg
    11521 ctatcagatg ttctgggaac actggctgcc agaacgtgcg ccggtcgatc agttgccggc
    11581 tctgctggag ggactctgtg caaaatacga aaccttcgac ggggacatga cagaatggat
    11641 gctttcccgt ttggtaggcc ggctgcttgt tcgcaccctc aatgagttgg gagagtccgc
    11701 cactgacgcg cagattttca attggctggg atctggagcc aacaagcgtg gttacttcaa
    11761 ccgggatcac gaatccctgc cgaaaatccg gcactggctt gaacgacatc ctgaacgata
    11821 caaggcagtg cttgctcaat gtgttcagac ggcaggagct gaccagcgtc attaccagca
    11881 cttgcgttta catgatgcaa cgccacaagc agacataggc ctctggcatc tacagcaggc
    11941 gggcacgttg gaggaggctg cagctagagt ccacttgcag aatgcggtca acgcacttat
    12001 gtggcaacgc ggcgccgagg ggttgtcgct ggaggccatc gaggcttggg gacgggcatt
    12061 cccagagcgg gcagaatggc tgaatgactg gcttgtctgc gacctagagc gggagagttg
    12121 gcacctggag gatgcggctc gtacccggcc atacagagaa cggcaagccg agcagcgtcg
    12181 ccagcgaacc cagcacatta ctcccgagct atccgcaatc tctgcaggca cagcttcggc
    12241 acgcctgctt gagcacctgg cgatgggctg gctgaacggc ttctcggata tccacgggga
    12301 aacgatcagc gcgcgctttg ccaactacag cgatctggga gatgagctga tgctcgctgc
    12361 cgaatccggc ttccgtcatt gtctgcagcg tcctgatcta ccctcggccg cagaagtcat
    12421 cgcgctggat caggctggtc aacgccactt catcagccaa ccctgcctgc ttggcctgca
    12481 attgaactgg gtagatgcac ccgagcaggt agctacgctg cctgatgatc tggttgagcg
    12541 gctgctggcc ctctatttct gcagtccaca tttcgggttg gacgacctgg atggctgggc
    12601 cgcacagacc gcgcaacagc gtcccgagct atttgctcgt gtgctggttc accaagcctc
    12661 tgccgctttt tctgctggca atgtctctgc aggtatggtg cactttttgg gcagagaggc
    12721 tgcatatgcg caggtcgcgc aacaggcaag tcctgaatta ctgcggcagt tccccatgaa
    12781 cgctgccggg cagcagttgc gtaatctccg gcagttgctg ggggctgctc tgttgagagc
    12841 acctgaatgt cttgctgggc ttgctacgca taagctggag tcaccacagc ttcatgttga
    12901 gcaacagcta ctgtggctgg cgatacaggt gctgaccgga cacaccgaag gtgaagaaga
    12961 attctggcag cgtcttggca aggcagaggc agccgatgta gcgatgcttc tgactgttga
    13021 aatcgtgacg caaggtattc aactgcaggc actgcaaacc ccacagccag agcgcattct
    13081 gggacggttg atcgagtcga tagccccaca tgccgagcta gagcgcagca gaggtggggt
    13141 tgtcactacg gcaatggatc ttggcgacga actgcgcggc atgagcgact ggcttggtgc
    13201 gcagcctacg gcagaggcca gtggcgagtt gcggcgcctg cttgcttgcc caacgctggc
    13261 agcgcagcgc tacaggattc agagcacact ggagcagcaa caggcgcacc gacgagaagc
    13321 cgaattccaa ttccttgacc tcaaggcagt ggccgatgtg ctggccaatc gggggccggc
    13381 gaatgcggcg gatctggctt ggctgaccct gtctcacctc gatgacattg cccatgaact
    13441 acacagtgac aacgacgacg gctatcgggc gttctggaat gtctggacag agaacaaaac
    13501 cacacataaa aatcgacgcg gggaaaacct gtgccgcgat gtcctgctga cccgcctacg
    13561 ctcgcgcctg aatgctcacg gcatcaacat cgccccggaa tttgaccatg ctggagacaa
    13621 gcgcgccgat ctccatctgg actaccgcaa ccagttggcc ctaccgatcg aaatcaagcg
    13681 cgatgaccat gacaaactct ggacagcact gcgcgaccaa ctgattgccc agtatgtgca
    13741 tgcaccgaag tcggcaggcc acggcatcta tctggtgttc tggtttggtg aaagcagcag
    13801 gctgaaaagc ccccctaacc gggaaccaaa gccggcgact ccggatgagc tgaaaaccaa
    13861 actcgaagct cagctgacag aggaggagcg ccggagggtg tttgtgcgag tgctggacgt
    13921 cagttggctc taacttcagc tgacaccggc gcagcgacag gcgcggaggt cgatcgctag
    13981 cacgcagacc gccccgtttc tgcaggcgca gcagcgtgag gatattcgcc ccacctgatg
    14041 acaaacgccc cgcaatcgcg gggcgtttgt tttgtagggt ggatggcctc ccccgaggat
    14101 gtcctccccg tggaaaactc gcaccgcgat ttccaccccg ccccgacgca cccacggtag
    14161 aaaagacctg cggtcgtttt ctaccctacg actttcctgc gccgctcgtc cggcggattc
    14221 gcctttggcc aattccgacc ttcacttgtc ctcgggggac catccatcac ctgagggtta
    14281 ccgacatgct catccgaacc attgccctgc tgctggcggt gctctgctcc ccgctggcct
    14341 ggagccaacc ccccgcagct cccgccgccg cagccgccag cacggacgcc gcactgacca
    14401 cccgcctggc catgcgcgac ctgtgggtcg ggcacatctt ctgggtcagg aactacgcgc
    14461 tggccaacca gagcggcaac gccccgcagg ccgacacggc cgccaaggaa gtggtggcca
    14521 acgccacggc gatcgccaac agcctgcagc cgctgtacgg ccagccagcc gccgaccaga
    14581 tgctcaagct gctggccggc cactgggggg cgatcaagga ctacagcgac gccggcgtgg
    14641 ccaaggacgc ggcgaaacag aaagccgcca tggccgccct ggcgagcaac gccaaggcga
    14701 tcgccgcctt cctcgccaag gccaacccct acctaccgga agacaccctg gtgaccctgc
    14761 tcagcgccca cggcgggcac cacatcagcc agatcgacca gctggccaag gccgactacg
    14821 ccggcgaggc gaagacctgg cacgccatgc gtgagcacat tctcgtcatt gccgacgccc
    14881 tggccggggc gctggtcaag cagttccccg agaagttctg agtagcccgc ggggcgcctt
    14941 gcgcgccccg cggcagtttc agtcgcgcgc ccacacctcg taggcatgcg cgggcgtttc
    15001 cgccgcggcc gcctgctcgt cgcgactgag cagctgccag tcgtcctcgg caaactcggg
    15061 gaagaaggca tcgccttccg gtgccagatg cacacgggtc aggtacagac gctgcgccag
    15121 cgccagcccc tgggcgtaca gctcggcgcc gccgatcagc atcagctcgt cgacgccccg
    15181 ttcccgcgcc cattcatcgg cgcgggcgat ggccgccggc agagaggcga acacctcggc
    15241 gccgtcgagc gtgaggccgg cctggcggct gaccaccagg ttgagccgcc ccggcagtgg
    15301 ccggccgagc gaatcccagg tcttgcgacc catgatgatc ggcttgccga gggtcacggc
    15361 cttgaagtgc ttgagatccg ccggcaggtg ccagggcaga cggttgtcga tgccgatcac
    15421 gcggttgtcg gcgagcgcgg cgatcagggc gagggggagc ggtgtcgtgt tcatggcggc
    15481 gaggatagca gccggcgggc gccgctgacg atggcgcggg gcctttgcgg tatcctgcgc
    15541 agcatcagac agacacatgg agttgtgatg gccaccctgc tcacctcgct caacggctgc
    15601 ccggccacaa gcggcgaacg aaaggtcgct agcttgttcg accgcctgct cgacgaggac
    15661 tacctgtgct ggttcgacgt gccgctcggc ccgcgcggcc agcgtccaga cttcaccctc
    15721 ctgcacccgc tgcgcggcat cctgctgctg gaggtcaagg actggaagct gtctaccctg
    15781 cacaagctcg accgcaagca ggccaccctc atactgccct caggacccaa acaggccagc
    15841 aacccactgg agcaggtgcg ccagtactgc cacgtgctgg ccggcctgct gcagcgtgac
    15901 ccgcagctgg tccaccggga cggtccccgg cagggccagt tgctgctgcc ctggggccac
    15961 ggggtgatcc tcacccagat cagccgcgcg cagttcgacg cggccggcct cggcgaggcc
    16021 atgaaccccg cccaggtgat ctgcaaggac gaactgggcg aggaccagga cccggaaacc
    16081 ctgcaggccc gcctgtggga catgttcact gtcgccttcc cctgcaacct cggccccgcc
    16141 caggtcgatc gggtgcgctg gcacctgttc cccgaactgc gcctgcgcag ctcgcagcag
    16201 agcctgttcg aggacgcccc cgagcccggc cagccgccgg ccgcggcgcc cgacctgatc
    16261 cgcatcatgg acctgcagca ggagcagctg gcgcgcaacc tcggcgaagg ccaccgggtg
    16321 atccacggcg tggccggctc gggcaagacc atgatcctcg gctaccgcgc cctgcatctg
    16381 gccaggctgc tgcacacgcc gaccctggtg ctgtgcttca acaaggcgct ggccgcacgc
    16441 ctggccctgc agatggacgg ccatggcgtc ggcgagaagg tccaggtgcg ccacttccac
    16501 gcctggtgcg gcgagctgct gcgccggcat ggcctcgccc tacccccgcg cggcagcgcc
    16561 agcgcataca acgagcaact ggtggcgcaa actctcgaag ccgtcgccgc cgggcggatc
    16621 ccgaccggcc agtacggcgc catcctgatc gacgagggcc acgacttcgc cccggagtgg
    16681 ttccaactgg ccacgcagca ggtcgacccc gccagccagt cgctgctggt gctctacgac
    16741 gacgcccaga gcctgtacca gcggcgcaag ttcagcttct ccagcgtcgg catccaggcc
    16801 cgcgggcgca ccaccatcct gcgcctgaac taccgcaaca ccgccgaaat cctccaggtc
    16861 gccgcgcgct tcgccggcga cgggctggcc gagcagaccg ccgaggaaga cggcatcccg
    16921 cgggtgccac cgcagagcgt cggccgccac ggcccgcagc cgctgtggcg cgaatgcgcc
    16981 g
//
LOCUS       DATCUN010000019         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_632, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000019 DATCUN010000000
VERSION     DATCUN010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VIO83_00670"
     CDS             <1..268
                     /locus_tag="VIO83_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329836.1"
                     /translation="RSAGSEFLETRFGSRELRITVTMQPDRLVTDQALPEGDAVQVTV
                     LENPFRLTRDLTNILGSAKSGDALVLVCAHAGLYRSALKFLGVV"
     gene            complement(340..918)
                     /locus_tag="VIO83_00675"
     CDS             complement(340..918)
                     /locus_tag="VIO83_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HEY8329837.1"
                     /translation="MLRILLINDTAKKVGRLKSALTEAGFEVLDESGLTVDLPARVAA
                     VRPDVILIDSESPGRDVMEQVCLLSRDQPHPIVMFTDEHDPKVMRQAIQSGVSAYIVE
                     GIHSARLQPILDVAMARFESDQALRAQLNARDQQMAERKRIELAKGLLMKMRQCNEED
                     AYTLMRRQAMSKQQKLIQVAEQIIAMHEMLGH"
     gene            complement(936..2147)
                     /locus_tag="VIO83_00680"
     CDS             complement(936..2147)
                     /locus_tag="VIO83_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8329838.1"
                     /translation="MPDHTPAPRHDALSWVAGSDAPEKNVLDLGFMALADSASLIVAA
                     TQGFAQPYGLTLNLRRQASWAGLRDKLVSGELDAAQMLYGQVYGTHLGIGSPATPMAI
                     LMGLNQNGQSINLSTALREAGVTTGEALRERTHQSGAKLTFAHTFPTGTHALWLYYWL
                     ASHGIHPLSDVNSVVVPPAQMVTHLRAGRIDGFCAGDPWGAHAVDEGQGFTVATSQSI
                     WADHPEKVLGVTREFVEQCPNSARALTMALLEASRFIDASEENRRSTAQLLSAAEFVN
                     APVASLEPRFLGHYQDGLGHAWLDSHPLRFFAAGTVNMPYLSDGIWFLTQLRRWGLLS
                     DDPAYHEVAGEVHQLDLYREAAEALGIAVPQPLRSSTLFDGKVWDGSDPAGYARSFAL
                     HALAERQKLAL"
     gene            2372..>3707
                     /gene="xdhA"
                     /locus_tag="VIO83_00685"
     CDS             2372..>3707
                     /gene="xdhA"
                     /locus_tag="VIO83_00685"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003175495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HEY8329839.1"
                     /translation="MIQFLLNRELRCETALDLNLTVLEYLRRHLGRSGTKEGCASGDC
                     GACTVVVAEPDPAANGERLRYRSLNACLTFMSALHGKQLLSVEDLGQPGHLHGVQQAM
                     ADCHGSQCGFCTPGFVMSLFALHKGGEGYDRAQAVDALTGNLCRCTGYRPILAAAERA
                     CTNRQTDTFDRHAAETLAALHAIASQDEAGLARGERRCWLPQTLDELAEIYLAQPQAR
                     LLAGGTDLALEVTQQHRTLPLLIDLGRVPELKRIALTDSHVEIGAGVALSDCQAVLAG
                     EYPDFGALLARFASRQIRNRGTLGGNLGNASPIGDTPPLLIALGAELLLRCGPVRRTL
                     ALEEFFRDYRVTALAPGEFIECIRVPRATPGQAMRAYKVSKRLDDDISAVCVAFNLQL
                     RDGVVSEARIAFGGMAATPRRAFQCEQALLGTCGDAVAIEQACQALAQEFTPL"
BASE COUNT          544 a         1214 c         1340 g          609 t
ORIGIN      
        1 gcgctcggcc ggaagcgaat tcctcgagac ccgtttcggc agccgggagc tgcggatcac
       61 ggtgaccatg cagccggatc ggctggtcac cgaccaggcg ctgccggagg gtgacgcggt
      121 gcaggtgacg gtgctggaga acccgttccg cctgacccgc gacctgacca acatcctcgg
      181 ttcggcgaag tcgggcgatg cactggtgct ggtgtgcgcc cacgccgggc tctaccgctc
      241 ggcgctgaag tttctcgggg tggtctgaag cggagttggt cgccggtggg ccccctcagg
      301 tacggggcag ggtgcgcagc gcgcaccctg cgtgcgacgt cagtggccca gcatctcgtg
      361 catggcaatg atctgctcgg ccacctggat cagcttctgc tgcttgctca tcgcctggcg
      421 gcgcatcagc gtgtaggcgt cttcctcgtt gcactggcgc atcttcatca gcaggccctt
      481 ggccagctcg atgcgcttgc gctcggccat ctgctggtcg cgggcgttga gctgggcgcg
      541 cagggcctgg tcgctctcga agcgcgccat ggcgacgtcg aggatcggct gcaggcgcgc
      601 ggagtggatg ccttcgacga tgtaggcgct gaccccggac tggatggcct ggcgcatcac
      661 tttcgggtcg tgctcgtcgg tgaacatgac gatcgggtgc ggctggtcgc ggctgagcag
      721 gcagacctgc tccatcacgt cgcggccggg cgattcgctg tcgatcagga tcacgtccgg
      781 acgtaccgcg gcgacccgcg ccggcaggtc gacggtcagg ccggactcgt cgagcacctc
      841 gaagccggcc tcggtcagcg cactcttcag gcgtccgacc ttcttcgcgg tgtcgttgat
      901 caggaggatg cgcagcatgg acatgtctcc cgggctcaaa gggccagctt ctggcgttcg
      961 gccagcgcgt gcagggcgaa actgcgggca tagccggccg gatcgctgcc gtcccagacc
     1021 ttgccatcga agagggtgga actgcgcagc ggctgcggta cggcaatgcc cagcgcctcg
     1081 gcggcctcgc ggtacaggtc gagctgatgc acttcgccgg ccacttcatg gtatgccgga
     1141 tcgtcgctca gcaggcccca gcggcgcagc tgggtaagga accagatgcc gtcggacagg
     1201 tacggcatgt tcaccgtgcc tgcggcgaag aagcgcagcg ggtggctgtc cagccaagca
     1261 tggccgaggc cgtcctggta gtggccgaga aagcgcggtt cgaggctggc cactggggcg
     1321 ttgacgaatt ccgcggcgct cagcagctgg gcggtgctgc ggcgattctc ttcgctcgcg
     1381 tcgatgaacc ggctggcctc gagcagggcc atggtcaggg cgcgggcgct gttggggcac
     1441 tgctcgacga actcgcgggt gacgccaagg accttttccg gatggtccgc ccagatcgac
     1501 tggctggtgg cgaccgtgaa gccctggccc tcgtccacgg catgggcacc ccagggatcg
     1561 ccggcgcaga agccgtcgat gcgcccggcg cgcaggtggg tgaccatctg cgccggcggc
     1621 accaccacgc tgttgacgtc gctgagcgga tggatgccgt ggctggccag ccagtagtac
     1681 agccacaggg catgggtgcc ggtggggaag gtgtgggcga aagtcagttt cgcaccgctc
     1741 tggtgcgtgc gctcgcgcag tgcctcgcca gtggtcacgc cggcttcgcg cagcgccgtg
     1801 ctgaggttga tcgactggcc gttctggttg aggcccatga ggatcgccat gggcgtggcc
     1861 gggctgccga tacccaggtg ggtgccgtag acctggccat agagcatctg tgccgcgtcc
     1921 agttctccgc tgaccagctt gtcgcgcagg ccggcccagg aggcctggcg gcgcaggttg
     1981 agggtcaggc cgtagggctg ggcgaagccc tgggtggcgg cgacgatcag cgacgccgag
     2041 tcggccaggg ccatgaagcc gaggtcgagg acgttctttt ccggggcatc cgagccggcg
     2101 acccaggaca gggcatcgtg gcggggcgcg ggggtatggt cgggcatggc aggttccaga
     2161 gacgggcagg gcaggcgggc tgcatgtcgg caatcaagca agtcatatgc cagcagcgct
     2221 gaatggcgtg ccaacgcggg ctctagccaa ggtcgcgcgc gtgccgggca ggtgcggtca
     2281 tgtgcaccat ggcggagcgc ttgcccccat gcgccggcct cactacagtc gctgcaaggg
     2341 cttatcgaac aagccgaggc agggggccgc cgtgatccag tttctgctca accgcgagct
     2401 gcgttgcgag actgcgctcg atctgaacct gacggtgctc gaatacctgc gccgccatct
     2461 cggtcgaagc ggcaccaagg aaggctgcgc ctccggcgac tgcggtgcct gtaccgtggt
     2521 ggtcgccgag ccggacccgg ccgccaacgg cgagcgtctg cgctaccgca gcctgaatgc
     2581 ctgcctgacc ttcatgtccg cgctgcacgg caagcaactg ctcagcgtcg aggatctcgg
     2641 ccagcccggt catctgcacg gcgtgcagca ggcgatggcc gactgccatg gcagccagtg
     2701 cggcttctgt acccccggct tcgtgatgtc gctgtttgcc ctgcacaagg gcggcgaggg
     2761 ttacgaccgc gcgcaggcgg tcgatgcgct gaccggcaac ctgtgccgct gcactggcta
     2821 ccggccgatc ctcgccgctg ccgagcgggc ctgcacgaac aggcagacgg ataccttcga
     2881 ccgccatgcc gccgagaccc tggcagccct gcatgccatc gccagccagg acgaggccgg
     2941 gctggcgcgg ggcgagcggc gttgctggct gccgcagaca ctggacgagc tggcggaaat
     3001 ctatttggcc cagccgcagg cgcgcctgct ggccggcggt accgacctgg cgctggaggt
     3061 cacccagcag caccgcacct tgccgctact gatcgacctc ggccgggtgc ccgagctcaa
     3121 gcgtatcgcg ctgaccgaca gccatgtcga gatcggcgcg ggcgtggcgc tgagcgactg
     3181 ccaggccgtg ctggccggcg aatacccgga cttcggcgcg ctgctggcgc gctttgcctc
     3241 gcggcagatc cgcaatcggg gcaccctcgg cggcaatctc ggcaacgcct cgccgatcgg
     3301 cgacacgccg ccgctactga tcgccctcgg tgccgagctg ctgctgcgct gcggaccggt
     3361 gcgccgcacg ctggcgctgg aggagttctt ccgtgactac cgggtcaccg cgctggcgcc
     3421 cggcgagttc atcgaatgca tccgcgtgcc gcgcgccacg ccggggcagg cgatgcgcgc
     3481 ctacaaggtc agcaagcgcc tggacgacga catctccgcg gtctgtgtgg cgttcaatct
     3541 gcagctgcgc gatggtgtgg tgagtgaggc gcgcatcgcc ttcggcggca tggcggcgac
     3601 gcccagacgc gccttccagt gcgagcaggc cctgctcggc acctgcggcg acgcggtggc
     3661 catcgagcag gcctgccagg cgctggcgca ggagttcacc ccgctca
//
LOCUS       DATCUN010000020         1734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_75788, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000020 DATCUN010000000
VERSION     DATCUN010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75788"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VIO83_00690"
     CDS             complement(<1..891)
                     /locus_tag="VIO83_00690"
                     /inference="COORDINATES: protein motif:HMM:NF037004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail-specific protease"
                     /protein_id="HEY8329840.1"
                     /translation="MKAKRSSLLLALALLAPIALLARPDGDRVATRADAGVVPMGPTA
                     DQSTAAKLVYGILSDSRYAYRPLPLDDALSQDVYKHYLDALDPSKQFFTAKDIEGFMP
                     LATRLDEAIKSGQLTPAYDIFSVYRKRVGERVAHARSLVKDDHFDFTTDERWEYDREQ
                     APWAKDSGELDELWRKSVMNDWLRLKMAGKKPEAIVQTLDKRYANLLRSVGELDSEDV
                     FQTFLNAYTSAVDPHTDYFTPKTAEQFNQQMSLSLEGIGAVLQRQDDMVVIREIVPGG
                     PADLSRKLQPGARIVGVAQGD"
     gene            complement(1114..>1734)
                     /locus_tag="VIO83_00695"
     CDS             complement(1114..>1734)
                     /locus_tag="VIO83_00695"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016986123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY8329841.1"
                     /translation="LATTVAQMGLKYVVVTSVDRDDLRDGGAQHFADCISAIRSHSPG
                     TRVEILTPDFRGKGRMDRALEILATSPPDVFNHNIETVPDLYPNVRPGADYQWSLTLL
                     QKFKAQHRALPTKSGIMLGLGETLEQVQATLRDLRAHDVDMVTIGQYLQPTAHHHPVL
                     RYWTPEEFAELEHYGLALGFSHVASGPMVRSSYHADRQALQAGVAG"
BASE COUNT          277 a          553 c          603 g          301 t
ORIGIN      
        1 atcgccctgg gccacgccca cgatgcgcgc cccgggctgc agcttcctgg acaggtcggc
       61 cgggccgccg ggcacgatct cgcggatcac caccatgtcg tcctggcgct gcagaaccgc
      121 gccgatgcct tccagcgaca gggacatctg ctgattgaac tgctcggcgg tcttgggggt
      181 gaagtaatcg gtgtgcggat ccaccgccga ggtgtaggcg ttgaggaaag tctggaagac
      241 gtcctcgctg tccagctccc ccacgctgcg cagcaggttg gcatagcgct tgtccagggt
      301 ctggacgatc gcctcgggct tcttgccggc catcttcagg cgcagccagt cgttcatcac
      361 cgacttgcgc cacagctcat cgagctcgcc gctgtccttg gcccagggcg cctgctcgcg
      421 atcgtattcc cagcgctcat cggtggtgaa gtcgaaatgg tcgtccttca ccaggctgcg
      481 ggcatgggcc acccgctcgc cgacgcgctt gcggtagacc gagaagatgt catacgccgg
      541 agtcagctgg ccgctcttga tcgcctcgtc caggcgcgtg gccagcggca tgaagccttc
      601 gatgtccttg gccgtgaaga actgcttgct cgggtccagc gcatccaggt agtgcttgta
      661 gacgtcctgc gacagcgcat cgtccaaggg cagcggccga taggcatagc ggctgtccga
      721 gaggattccg tagaccagtt tggcggccgt ggactgatcg gcggtcggcc ccatcggcac
      781 cacgcccgcg tcggcgcggg tggccacccg gtctccgtcc ggccgcgcca gcaatgcaat
      841 gggggccagc aaggccaggg ccaacaggag ggaggagcgc ttggctttca ttcgatgggc
      901 tcgcggccgg cagggccgcc taaggcgtgc ggagaggggt gtcggtggtg cacatggacc
      961 gcatcacagt gtcgaaagtt gcgcaggctg tcatgtgcat ttcagcctag cggcgcgccg
     1021 gtatcgcaat gtgaacgcag ggcccggata caacgacgcc gccgggcctt cggccagacg
     1081 gcgcgtgaac ggatcgatgc gcgccaagcg tgctcagccg gcaacaccgg cctggagcgc
     1141 ctggcgatcg gcatggtagg acgagcgcac catcgggccg gaggcgacat ggctgaagcc
     1201 cagggccagg ccgtagtgct ccagctcggc aaattcctcc ggggtccagt agcgcagcac
     1261 cggatggtgg tgcgcggtgg gctgcaggta ttggccgatg gtgaccatgt ccacatcatg
     1321 cgcgcgcaga tcgcgcagcg tggcctgcac ctgctccagg gtctcgccca ggccgagcat
     1381 gatcccggac ttggtcggca gggcccgatg ctgggccttg aacttctgca gcagggtcag
     1441 cgaccactgg tagtcggcgc ctggacgaac gtttggatac aggtccggca cggtctcgat
     1501 gttgtggttg aacacgtccg gcgggctggt cgccaggatc tccagcgcgc ggtccatgcg
     1561 gcccttgccg cggaagtccg gggtcaggat ctccacccgg gtgccgggcg aatggctgcg
     1621 gatggcgctg atgcaatcgg cgaaatgctg ggcgccgcca tcacgcaggt cgtcgcggtc
     1681 cacgctggtc accaccacgt atttcaggcc catctgggcc acggtggtgg ccag
//
LOCUS       DATCUN010000021         4638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_19373, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000021 DATCUN010000000
VERSION     DATCUN010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19373"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2278
                     /locus_tag="VIO83_00700"
     CDS             <1..2278
                     /locus_tag="VIO83_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY8329842.1"
                     /translation="SAVVGYKRSLGADAPPCSYEDIEQSDCLLIAGSNMAYAHPVLFR
                     RLEAAKAQRPEMKVIVVDPRRTDTCELADLHLAILPGTDVALLHGILHILLWEGWIDR
                     AFIDAHTEGFDALKKLVRDYTPAAVADICGISVDNLQLCAELIGKAPSFLSLWCMGLN
                     QSSAGSAKNSALINLHLATGQIGRIGAGPFSLTGQPNAMGGRETGSLSNLLPGHREAG
                     NAAHRAEVASYWGVEQLPETTGLSAIELFEAVRVGKIKALWIACTNPAQSMPDQNKIH
                     EALAACPFVVVQEAFFTTETCRYADLLLPAASWGEKEGSVTNSERRISHVRRAVPAPA
                     EARPDWAIVCDFARRLEPQLRPGLPSLFDFSNPAALFEEYKQLTAGRDLDLSGISHAL
                     LDDLGPQQWPFPAGAVSGTPRLYGEGVFPTASGRAQFVADPYRAAQEKREARYSLTLN
                     TGRLRDQWHGMSRTGTAAQLFGHVEEATLSLHPDELQRRRLASGDLVKLRSRRGSLIL
                     PVQADDSIRPGQAFLPMHWGDRFLKGLGTNVLTLPACDPLSKQPELKHAGVEVDKVEL
                     PWQLFALIEGEVQERFEALRPLFESFAYASLTLTGRERPALLVKAARAVAPEAELLAR
                     IDALLGLTAGPVLAYDDPRRTVGKRVRIEDGRITAIRLAGETAARDWLKALWSEGKAD
                     TELRRWLLAPLSTPPGNAGACNSKTVCSCMNVSDAAIRTGIERGLDLDGLKQELKCGT
                     SCGSCVPEIKRLLARAPA"
     gene            2471..3250
                     /gene="cobA"
                     /locus_tag="VIO83_00705"
     CDS             2471..3250
                     /gene="cobA"
                     /locus_tag="VIO83_00705"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009047904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY8329843.1"
                     /translation="MSAKVWLIGAGPGDPELLTLKAVRALGEAAVVMIDDLVNPAVLE
                     HCPQARVIGVGKRGGCRSTPQEFIHRLMLRYARQGKCVARLKGGDPCIFGRGSEEADW
                     LAERGVATEIVNGITAGLAGATQCGIPLTLRGVARGVTLVTAHTQDDSSLNWSALAQG
                     GTTLVVYMGVAKLADIRDGLLAGGMRDAMPVAMIENASLAQQRECRSSLGAMQQDATA
                     FGLKSPAILVIGEVAAARMAELLPSLAEQPLPSLLAATGTR"
     gene            complement(3714..4601)
                     /locus_tag="VIO83_00710"
     CDS             complement(3714..4601)
                     /locus_tag="VIO83_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY8329844.1"
                     /translation="MLAELFAVMAPVLIVAGIGYAWVRSGQAYPTDFVARLVLNIGTP
                     CLVLSTLSRTEVDPQAFGQMALACVVVSLLMGLIGVVLSRSMAMDWKVLVPAYLFPNT
                     GNMGLPISLYAFGEQGLALAVAFFLMLSVAQFTLGMLLSGAERSWRKLLISPTIISLG
                     AALVVMLFDLELPRWLANTVNLLGGTAIPMMLITLGVSLASIRVQHLGRGMLLGALRI
                     LCGAGAGWLIGHLLDLSPLAQAVLVLQSAMPVAVFNYLFAVRANRSPEEVASLVMCST
                     LLSFVVLPLLLVWLLPTVR"
BASE COUNT          855 a         1659 c         1500 g          624 t
ORIGIN      
        1 ctcggcggtg gtcggctaca agcgcagcct cggcgccgac gccccgccct gctcctacga
       61 ggacatcgag cagagcgact gcctgctgat cgccggcagc aacatggcct acgcccaccc
      121 ggtgctgttc cgccgcctgg aagcggccaa ggcacagcgc ccggagatga aggtgatcgt
      181 ggtcgacccg cggcgcaccg acacctgcga gctggccgac ctgcacctgg cgatcctgcc
      241 cggcaccgac gtggcgctgc tccacggcat cctgcacatc ctgctgtggg aaggctggat
      301 cgaccgcgcc ttcatcgacg cccacaccga gggcttcgac gccctgaaga agctggtgcg
      361 cgactacacc ccggcggcgg tggcggacat ctgcggcatc agcgtcgaca acctgcagct
      421 gtgcgccgag ctgatcggca aggcaccgag ctttctgtcc ctgtggtgca tgggcctcaa
      481 ccagtccagc gccggcagcg ccaagaacag cgcgctgatc aacctgcacc tggccaccgg
      541 gcagatcggc cgcatcggtg ccggtccctt ctccctcacc gggcagccca acgccatggg
      601 cgggcgcgaa accggcagcc tgtccaacct gctgcccggc caccgcgagg ccggcaatgc
      661 cgcgcatcgc gccgaggtcg ccagctactg gggcgtcgag cagctgccgg agactaccgg
      721 cctgtccgcc atcgaattgt tcgaggccgt cagggttggc aagatcaagg cgctgtggat
      781 cgcctgcacc aacccggcgc agtcgatgcc cgaccagaac aagatccacg aagccctggc
      841 cgcctgcccg ttcgtggtgg tgcaggaagc cttcttcacc accgagacct gccgctacgc
      901 agacctgctg ctgcccgccg ccagctgggg cgagaaggaa ggctcggtga ccaattccga
      961 gcggcgcatc agccacgtgc gccgcgccgt gccggcgccc gcagaggcgc ggccggactg
     1021 ggcgatcgtc tgcgacttcg cccgtcgcct ggaaccccag ctgcgccccg gcctgcccag
     1081 cctgttcgac ttcagcaacc ccgccgcgct gttcgaggag tacaagcagc tcaccgccgg
     1141 ccgcgacctc gacctgtcgg ggatcagcca cgccctgctc gacgacctcg gcccgcagca
     1201 gtggccgttc cccgccggtg ccgtcagcgg cacgccgcgc ctgtacggcg aaggggtgtt
     1261 ccccaccgcc agcggccgcg cccagttcgt cgccgatccg taccgcgccg cccaggaaaa
     1321 gcgcgaggcg cgctattcgc tgaccctcaa caccggccgc ctgcgcgatc agtggcacgg
     1381 catgagccgc accggcaccg ccgcgcagct gttcggccac gtcgaggagg ccacgctcag
     1441 cctgcacccg gacgagctgc agcgccggcg tctggccagt ggcgacctgg tcaagctgcg
     1501 cagccgccgc ggcagcctga tcctgccggt gcaggccgac gacagcatcc gccccggcca
     1561 ggccttcctg ccgatgcact ggggcgaccg cttcctcaag ggcctgggca ccaacgtgct
     1621 gaccctgccg gcctgcgatc cgctgtccaa gcagccggaa ctcaagcacg ccggcgtcga
     1681 ggtggacaag gtcgagctgc cctggcagct gttcgccctg atcgagggcg aggtgcaaga
     1741 gcgcttcgag gccctgcgcc cgctgttcga gtcgttcgcc tacgccagcc tgaccctcac
     1801 cggtcgcgag cgcccggcac tgctggtcaa ggctgcccgc gccgtggctc ccgaagccga
     1861 actgctggcg cgcatcgacg ccctgctcgg ccttaccgca ggcccggtgc tggcctacga
     1921 cgacccgcgg cgcaccgtcg gcaagcgcgt gcgcatcgag gacgggcgca tcaccgccat
     1981 ccgcctggcc ggcgaaaccg ccgcccgcga ctggctcaag gcgctgtgga gcgaaggcaa
     2041 ggccgacacc gaactgcgcc gctggctgct cgccccgctg agcaccccgc cgggcaacgc
     2101 cggcgcgtgc aacagcaaga cggtgtgcag ctgcatgaac gtcagcgacg ccgcgatccg
     2161 caccggcatc gagcgcggcc tcgacctcga cggcctcaag caggaactca agtgcggcac
     2221 cagctgcggc tcctgcgtcc cggaaatcaa gcggctgctg gccagagccc cggcctgaac
     2281 cggtgatgaa ccgtagggtg ggttagaccg caggtcgtaa cccaccaacc ggccgcaagg
     2341 tcggcaatcg cgatgcctgg cggcatcgac ggtgggttac gccgcgaggc ggctaaccca
     2401 ccctacgcag accgatgaat gccttgccgg cgtggcaaca ccggcaagcg ataccagaga
     2461 ggtggtgaac atgagcgcaa aagtctggct gatcggtgcc ggcccgggtg acccggaact
     2521 gctgaccctc aaggccgtgc gcgccctggg cgaggccgcg gtggtgatga tcgacgacct
     2581 ggtcaacccg gcggtgctcg agcactgccc gcaggcgcgg gtgatcggcg tcggcaagcg
     2641 cggcggctgc cgctccacgc cgcaggagtt catccaccgc ctgatgctgc gctacgcccg
     2701 ccagggcaag tgcgtggcgc gcctgaaggg tggcgatccg tgcatcttcg gccgcggcag
     2761 cgaggaagcc gactggctgg ccgagcgcgg cgtcgccacc gagatcgtca acggcatcac
     2821 cgccggactg gctggcgcca cccagtgcgg catcccgctg accctgcgcg gtgtggcccg
     2881 cggcgtgacc ctggtcaccg cccacaccca ggacgacagc agcctcaact ggtcggcgct
     2941 ggcccagggc ggcaccacgc tcgtcgtcta catgggcgtg gccaagctgg ccgacatccg
     3001 cgacggcctg ctggccggcg gcatgcgcga cgccatgccg gtggcgatga tcgagaacgc
     3061 ctcgctggcg cagcagcgcg aatgccgcag cagcctgggc gccatgcagc aggacgccac
     3121 cgccttcggg ctgaaaagcc cggcgatcct ggtgatcggc gaggtggcag cggcgcgcat
     3181 ggcggagctg ctgccgagtc tggcggagca accgctgccg agcctgctgg cagccacggg
     3241 cacacgctga aggataggcc gcagccggca gcgcaagccc ggcatgccac gaaaccacga
     3301 aaccggacga acggtagcct caaattcagg ccaggggaac ggcctgccgc cgacgcctgc
     3361 aaaggctctg caacagggct atgcagaaga aaaccggcga ttcaagacgt acagattcag
     3421 atcaggaaac ttttttgcat caaaaacttc taaaccttta cgactacgac ctctagagta
     3481 tgctgttaca ggcaacggca agccgcaggg cattgtcgtt gatcaacgcc agcggcttgt
     3541 ttcagggtga taattccccg tttcaaccca gccaacaaca ataaaaggcg ctcgaaaatg
     3601 gtcaaattca atcccaagtg gcggcaggcg gccttggccg tgctggtcgt tacccttctg
     3661 ctggtgctac ccaatctgag ccgcatgatc agctgagccg cgtccagccg acttcagcgc
     3721 actgtgggca gcaaccagac cagcaatagt ggcaacacga cgaacgatag caacgtcgaa
     3781 cacatgacca gactcgccac ctcttcgggc gaacggttgg cgcgcacggc gaacaggtag
     3841 ttgaacacgg ccaccggcat agccgactgc agcaccaata ccgcctgcgc cagcggcgac
     3901 agatccagca gatggccaat cagccagcct gccccggccc cgcacagtat gcgcaacgca
     3961 ccgagcagca tgccccggcc aaggtgctgc acgcggatgc tcgccagcga cacgccgagg
     4021 gtgatcagca tcatcggaat ggccgtcccc cccagcaggt tgaccgtatt ggccagccag
     4081 cgcggcagtt cgagatcgaa cagcatcacc accagggccg cgcccaggct gatgatcgtc
     4141 gggctgatca gcagtttgcg ccaggagcgc tcggcgccgg aaagcagcat gcccagcgtg
     4201 aactgggcca ccgagagcat cagaaagaag gccactgcca gcgccagccc ctgctcgccg
     4261 aaggcgtaca ggctgatcgg caggcccatg ttgccggtgt tggggaacag gtaggccggc
     4321 accagcacct tccagtccat ggccatgctc cggctcagga ccacaccaat cagccccatc
     4381 agcagcgaca ccaccacgca ggccagagcc atctggccga aagcctgggg gtcgacctcg
     4441 gtgcggctga gggtcgacag caccagacag ggcgtgccga tgttcagcac caggcgggcg
     4501 acgaaatcgg tggggtaagc ctgcccggaa cggacccagg cgtagccgat cccggcgacg
     4561 atcagcaccg gcgccatcac ggcgaacaac tcggcaagca tgagccttcc ttgcggcaaa
     4621 gatggcgatg ctacgcgg
//
LOCUS       DATCUN010000022         5867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_38249, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000022 DATCUN010000000
VERSION     DATCUN010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38249"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VIO83_00715"
     CDS             complement(<1..407)
                     /locus_tag="VIO83_00715"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454362.1"
                     /note="catalyzes the S-adenosylmethionine-dependent
                     transmethylation of thiopurine compounds; may be involved
                     in selenium cycling by forming dimethylselenide and/or
                     dimethyldiselenide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HEY8329845.1"
                     /translation="MHEEFWQARWARNEIGFHRSEVNPALQEYWPALQLAAASEVLVP
                     LCGKSLDMPWLAAQGHAVLGVELAERAVQDFFAEQQLVAQVGADGALRRYQAQGIAVL
                     QGDFFAVTPAQTAGCAGYYDRAALIALPPEMRRR"
     gene            518..1285
                     /locus_tag="VIO83_00720"
     CDS             518..1285
                     /locus_tag="VIO83_00720"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019092872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEY8329846.1"
                     /translation="MLPSLFISHGSPMVALQPGDTGRQLARLARELPRPKAILVVSAH
                     WETSDLRLTSAPRQQAWHDFYSFPPALYALDYAPPGAPQLAMEILELLEKAGVPAQLD
                     PQRPLDHGAWVPLRLMYPDADIPVVQLSLPSHQGPALQTRLGRLLAGLRGQDLLLIGS
                     GSITHNLRDLDWNAGQEVVEPWAREFRDWIVEQLSSGDEAALHDYRQQAPWAVRSHPS
                     DEHLLPLFFARGAGGQFHVEHQGFDKGTLGMDIYRFG"
     gene            complement(1422..3139)
                     /locus_tag="VIO83_00725"
                     /pseudo
     CDS             complement(1422..3139)
                     /locus_tag="VIO83_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251530.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(3398..4144)
                     /locus_tag="VIO83_00730"
     CDS             complement(3398..4144)
                     /locus_tag="VIO83_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEY8329847.1"
                     /translation="MSEYHQLAEVHDDYGVIRVVQAGDYRFLEFGEDVEQSCSYVPDP
                     AWLEYDYTRAMLLGALLPERAERALFLGLGGGSLTTTCLKFLSLQAVEAVELRPAVPQ
                     LAREFLGLADDPRLTIHIGDARTRLSDCAPADLIYLDLYTDTGPAPAHIAWGFLEACR
                     AQLRPGGWLVINQWSATDGKPLGAPLLRGLFRHCYWECPVTEGNVILYVPADGEQELD
                     TERLRQRAAALAPRLGYGLSRHIDALRPAS"
     gene            4286..5632
                     /locus_tag="VIO83_00735"
     CDS             4286..5632
                     /locus_tag="VIO83_00735"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEY8329848.1"
                     /translation="MSQTWSPESWRSKPIQQQPVYPDAAHLARVEQTLAGYPPLVFAG
                     EARELRRQFAEVTQGRAFLLQGGDCAESFAEFSAGKIRDTFKVLLQMAIAMTFAAGCP
                     VVKVGRMAGQFAKPRSSGEETIDGMTLPAYRGDIVNGIGFNEKNRVPDPERLLQAYHQ
                     STASLNLLRAFAQGGFADLHQVHQWNLDFIASAELTEKYGQLADRIDETLAFMRACGL
                     ENAPQVRETSFFTAHEALLLNYEQAFSRRDSLTGDWYDCSAHMLWIGDRTRQLDGAHV
                     EFLRGVGNPIGVKVGPSMDSDELIRLIDILNPDNDPGRLNLIVRMGADKVEQHLPRLI
                     RAVQGEGRQVLWSSDPMHGNTIKASSGYKTRDFAQVLNEVKQFFQVHQAENSYPGGIH
                     IEMTGQNVTECIGGTRPITEAGLSDRYHTHCDPRLNADQSLELAFLIADTLKQIRR"
     gene            complement(5691..>5867)
                     /locus_tag="VIO83_00740"
     CDS             complement(5691..>5867)
                     /locus_tag="VIO83_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019409286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEY8329849.1"
                     /translation="TYGAHGQVDWLTGIPLAVGGLLSISWGVKLAHGLPQRLLRVLFT
                     LFLLLCALLLGLKA"
BASE COUNT         1048 a         2062 c         1867 g          890 t
ORIGIN      
        1 cgacggcgca tctccggcgg cagggcgatc agcgccgcgc ggtcgtaata gccggcacaa
       61 cccgccgtct gcgccggcgt gacggcgaag aagtcgccct gcagcacggc aatgccctgc
      121 gcctgatacc tgcgcaaggc gccatcggcg ccaacctgcg ccaccagctg ctgctcggcg
      181 aagaagtcct gcacggcccg ctccgccagc tccacgccca gcaccgcatg cccctgggcg
      241 gccagccagg gcatgtccag gctcttgcca cacagcggca ccagcacctc gctagcggcc
      301 gccagctgca gcgccggcca gtactcctgc agcgccgggt tcacctcgct gcggtgaaaa
      361 ccgatttcgt tgcgcgccca gcgcgcctgc cagaactcct cgtgcatcgg ggcctctctt
      421 tgattgcctg attcgatcct aaaggtcgaa aacttatact ggatttcgat ggttcgtctg
      481 ccgaagataa caacatcgtc atccagagac acctgtcatg ctgcccagtc tgttcatttc
      541 ccacggctcg ccgatggtcg ccctgcaacc gggcgacacc ggccgccagc tcgcccgcct
      601 ggcccgcgag ctgccacgcc ccaaggcgat cctggtggtt tccgcccact gggaaaccag
      661 cgacctgcgc ctgacctccg cgccacggca gcaggcctgg cacgacttct acagcttccc
      721 gccggcgctc tatgcactcg actacgcacc gccgggtgcg ccgcagctgg ccatggagat
      781 cctcgaattg ctggagaaag ccggcgtgcc ggcgcagctc gacccgcagc gtccgctcga
      841 tcatggcgcc tgggtgccgc tgcggctgat gtatccggat gccgacattc ccgtcgtgca
      901 gctgtcgctg cccagccacc aggggccggc gctgcagacc cgactgggcc gtctgctcgc
      961 cggactgcgc gggcaagacc tcctgctgat cggctcgggc agcatcaccc acaatctgcg
     1021 cgacctggac tggaatgccg gccaggaagt ggtcgaaccg tgggcccgcg agtttcgcga
     1081 ctggattgtc gaacagctga gcagcggcga cgaggccgcc ctgcacgact accgccagca
     1141 ggcaccctgg gcggtgcgca gccacccgag cgacgagcac ctgctgccgc tgttcttcgc
     1201 ccgcggcgcc ggcgggcagt tccacgtcga gcaccagggc ttcgacaagg gcaccctcgg
     1261 catggacatc taccgcttcg gctgaccggt tgccgcccag ccgcccagcc gccggcacag
     1321 cggatcaggg cggcacagga gcagcaggca cctgaagctc cgtcgacggc acaaacaaaa
     1381 acgccccgca ctaggcgggg cgttttgctg tcacgagatc atcactcttc gtgatagcga
     1441 cgcaggcgca gggccttgcc ggcgacacgg gtttccttca ggcgacccag aacacgctcc
     1501 aggccctctt ccggcagctc caccagactg aaggtctcgc gcacctggat gcgaccgatg
     1561 gcctcgcgca ccagcttgcc ctcgttgatg atggcgccga gcaggttctt ggcggcgata
     1621 ccgtcgcgct tgcccagcgc ggtacggcag cgcacgcggc cttcagccag cggctccatc
     1681 ggacggcgcg gacgggcttc gccgctgcgc tcgccagtac gctcgacccg atcaccggcc
     1741 ggacgctcgc gggccggcgc cggtgctgcc ggctgctcgc ggcgcacgct ttccagatcc
     1801 agggcctggc cctgggtcag cttggccagc agggcggcag ccagttgctc cggagtgcac
     1861 tccaggcgac ggcacagctc ggcaccgatc tcgccacggg cagcctcggc gccgtccagc
     1921 agcggcttga ggctgttggt cagacgctgc agacgcgctt ccagcacggt ttcggcatcc
     1981 ggcatcttga tctcggcaac cttctgccca gtcacacgtt cgatcacctg cagcatgcgg
     2041 cgctcgcgcg gggtcaccag cagcagcgcg cgaccggtac ggccggcacg gccggtacga
     2101 ccgatgcggt gcacgtagga ctccgggtcg tacggcatgt cgatgttgaa cacgtgggtg
     2161 atgcgcggca cgtcgagacc gcgggcggcg acgtcggtgg caatcacgat gtccagggtg
     2221 ccgtccttga gcgactcgac cacacgctcg cgctgggcct gggcgatgtc gccgttcagc
     2281 gcggcggcgc ggtagccgtt gcgctcgagc atctcggcga tgtcgagggt ggcctgcttg
     2341 gtgcgcacga agccgatcag cgcatcgaac tgctcgactt ccagcagacg cagcacggcg
     2401 gcctgcttct ggtcagcatg caccatcagg tgggcctgct cgatgctctc cacggtctgg
     2461 gtcttggtgg cgatcttgac gtgcttcggc gacttcaggt tgcgctcggc gatggcacgg
     2521 atcgaggccg gcagggtcgc ggagaacagc acggtctggc ggctttccgg cagggcctgg
     2581 aagatcgact cgatgtcttc catgaagccg agcttgagca tctcgtcggc ctcgtcgagg
     2641 accaggcgat cgacggtggc cagcagtttc tcatcgcgac gcaggtggtc gcacaggcga
     2701 cccggggtcg ctaccactac ctgggcaccc tggcgcaggg ccttgagctg cggccccatg
     2761 ggcgcgccgc cgtagaccgc cagcaccttc acgccaggca tgtcgctgga gtagcgctcg
     2821 aaggccgcgg cgacctgcag ggccagctcg cgggtcgggg tcaggatgag gatctgcggc
     2881 tcgcggcggg ccgggtcgag cagcgacagc agcggcaggg cgaaggccgc ggtcttgccg
     2941 gtaccggtct gcgcctggcc gatcatgtcg tgaccggcga ggattaccgg aatcgactgg
     3001 gcctgaatgg cggacggctc ttcgtaaccc accgcagtga tcgcggaaag cacagccggg
     3061 tgaagattga gcgcggcgaa gccgccaacg atagtttcgg tcatgggtat ttgcctcatg
     3121 tacatccgca aagacccatg gccagcgctg cgccagccaa aagcaagacc caaagggtca
     3181 ccctggcagc ctggtcggcg gggatttgcg aaatcgcgtg aaaaggatag ttcgtcaaga
     3241 ggtgtccgtg gagacggacg cacggcggaa gcagcgcttc ctgatggtgc agagtttgaa
     3301 cagggcgcgt caaagccggc gcgcataata acgcaaaata tgtgccgagt cgcgctattt
     3361 ttaccccaca cgtccggaaa aacccgtcgc agagcgttca gcttgccggg cgcaacgcat
     3421 caatatggcg actcaagcca taacccagcc gcggcgccag cgctgcggca cgctggcgca
     3481 gacgctcggt atccagttcc tgctcaccgt cggccggcac atacaggatg acgttgccct
     3541 cggtgaccgg acactcccag tagcagtggc ggaacaggcc gcgcagcaag ggcgccccca
     3601 atggcttgcc atccgtggcg ctccactgat tgatcaccag ccagccgccg gggcgcagct
     3661 gcgcgcggca agcctcgaga aagccccagg cgatgtgggc cggcgccggc ccggtgtcgg
     3721 tatacagatc cagatagatc aggtcggccg gcgcacaatc ggacaggcgg gtgcgcgcat
     3781 cgccgatatg gatggtcagc cgcggatcgt ccgccagccc gaggaactcg cgcgccagct
     3841 gcggcacggc cggacgcagc tcgaccgcct ccaccgcctg cagggacaag aacttcaggc
     3901 aggtcgtggt caaactgccg ccacccagac cgaggaacag cgcgcgctcc gcgcgctcag
     3961 gcagcagcgc accgagcagc atcgcgcggg tgtagtcgta ttccagccag gccggatcgg
     4021 ggacgtagct gcagctctgc tcgacatctt cgccgaactc gagaaagcga tagtcaccag
     4081 cctgcaccac acggatcacc ccgtagtcgt cgtgcacctc ggccagctga tggtattcgc
     4141 tcatttcgtc cccccggaaa tttgcccggc atcctagcag accgcggccc atcgatgcag
     4201 ccaggctatc aggaccatca cccccgaggc tgcacctgat cggttaacat ggaaaacatt
     4261 cgccatccgc aagccgagtc gttcgatgag ccagacctgg agccccgaaa gctggagaag
     4321 caagccgatc cagcagcaac ccgtttaccc cgatgccgcg catctggctc gcgtggagca
     4381 gaccctcgcc ggctatccgc cgctggtgtt cgccggcgaa gcgcgcgagc tgcgccggca
     4441 gttcgccgaa gtcacccagg gccgggcgtt cctgctgcag ggcggcgact gcgccgagag
     4501 cttcgccgag ttctccgccg gcaagatccg cgacaccttc aaggtgctgc tgcagatggc
     4561 catcgccatg accttcgccg ccggctgccc ggtggtcaag gtcgggcgca tggccggcca
     4621 gttcgccaag ccgcgctcct ccggcgaaga aaccatcgac ggcatgaccc tgcccgccta
     4681 ccgcggcgac atcgtcaatg gcatcggctt caacgagaag aaccgggtgc cagacccgga
     4741 gcgcctgctg caggcctacc accagtccac cgccagcctc aacctgctgc gcgccttcgc
     4801 ccagggcggc ttcgccgacc tgcaccaggt gcaccagtgg aacctcgact tcatcgccag
     4861 cgccgagctg acggaaaaat acggccagct ggccgaccgc atcgacgaga ctctggcctt
     4921 catgcgcgcc tgcgggctgg agaacgcccc gcaagtgcgc gaaaccagct tcttcacggc
     4981 ccatgaagca ttgctgctca actacgagca ggccttctcc cgccgcgaca gcctgactgg
     5041 cgactggtat gactgctcgg cgcacatgct gtggatcggc gaccgcaccc gccagctcga
     5101 cggcgcccac gtcgagttcc tgcgcggtgt cggcaacccg atcggggtca aggtcggccc
     5161 gagcatggac agcgacgagc tgatccgcct gatcgacatc ctcaacccgg acaacgatcc
     5221 gggccgcctc aacctgatcg tgcgcatggg cgccgacaag gtcgagcagc acctgccgcg
     5281 actgatccgc gccgtgcagg gcgaaggccg ccaggtgctg tggagttcgg acccgatgca
     5341 cggcaacacc atcaaggcca gcagcggtta caagacccgc gacttcgccc aggtgctgaa
     5401 cgaggtgaag cagttcttcc aggtccacca ggcggagaac agctaccccg gcggcattca
     5461 catcgaaatg accggccaga acgtcaccga atgcatcggc ggcacccggc cgatcaccga
     5521 ggccggcctg tcggaccgct accacaccca ctgcgacccg cgcctcaacg ccgatcagtc
     5581 gctggagcta gcattcctga tcgccgacac cctgaagcag atccgccgct gacctgccaa
     5641 gggtagcggt gcgcgtcgcg caccctaccc ttgccccaag ctggcggcac tcaggccttg
     5701 agccccagca gcaacgcaca cagcagcagg aacagggtaa acagtacgcg cagcagccgc
     5761 tgcggcagcc catgcgccag cttcacgccc cagctgatgc tgagcagacc gccgaccgcc
     5821 agggggatgc cggtcaacca gtcgacctga ccgtgggctc cgtaggt
//
LOCUS       DATCUN010000023         6283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_56993, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000023 DATCUN010000000
VERSION     DATCUN010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56993"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..365)
                     /locus_tag="VIO83_00745"
     CDS             complement(45..365)
                     /locus_tag="VIO83_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008054509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2388 domain-containing protein"
                     /protein_id="HEY8329850.1"
                     /translation="MRRTLLVAALTFFLPVGAILAADVDWEDVATSAGVSASLYSTFK
                     DEKLVLAAEDDASSFVASGGAIRGAYLEAALQQIRRDHPGLKASDEELASAMLANAQD
                     GAPR"
     gene            complement(588..664)
                     /locus_tag="VIO83_00750"
     tRNA            complement(588..664)
                     /locus_tag="VIO83_00750"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(628..630),aa:Ile,seq:cat)
     gene            complement(772..3786)
                     /locus_tag="VIO83_00755"
     CDS             complement(772..3786)
                     /locus_tag="VIO83_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004352229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8329851.1"
                     /translation="MPRLPALPVLCLAVLPAYSHAALPGWTRDPWLLLAPYAAALLLT
                     LLALGLLLRGRQRRQLERLQRALQARAAHDRELLERAPLVIWEMCRHDLRFTQISAPD
                     EALLGYPVNAWLQADFCSRTLYPPDAPAALAFLRGEGEPAQARHECRLIAADGRLIRL
                     LAIVQRRGAGDDATLQGLFIDIGENPLSEQALRQSEQQYRSAFHDCPEIMLLIERHSG
                     RILAANRAFEEATGLPADTACGHTTSELGLWRQHEQGRRVLHQLHSHDLQNVEVTLIR
                     RGNEDFTGLLSARRVELDGQPAAIVVLRDLSALHHTRQLLHASEEKFAKAFYASPDAL
                     SITRLRDGRLLEANPGFTQITGYSPSEAINHSTLELGLWVDPADRQRLMRQLEQDGSA
                     QHLSARVRTRDGQERLCEFSAQPLQIDGEDCALIIARDISERMQMQERLQQAATVFES
                     TAEGVMITDLEQRIVAINRAFSAITGYSESEALGQTPQLLASGQHGQDFEEEIRSSLE
                     RDGHWQGEIWSRRKNGESYPAWMTISAVRNGGNRISHHVAVFADITSLKHAQARLDYQ
                     AHHDPLTGLPNRLLFENRLQLALDEAQTEQRRGAVLFLDLDRFKHINDSLGHPVGDLL
                     LQGIAKRLREQLREVDTVARLGGDEFIVLMPSVSQTSDIERVASKLMSAFSTPFNADG
                     HEFFMSSSMGISLFPEHGQDVATLVKNADSAMYLAKARGRNRIEFYTADLSFLATERM
                     TLENDLRRAAERGELHLHYQPKQCLNSGRLVGAEALLRWQHPLHGDVPPERFIPLAEE
                     NGSIIELGDWVLQEACRQMSVWQAQYLAFGALAVNLAGPQLRQPQLVGRISELLASHG
                     LEPASLQLEITETFVMSQKEEALPILQALKELGLQLAIDDFGTGYSSLSYLKRLPIDT
                     LKIDQSFVAGLPDDPNDAAITRAIIALGHSMQLTVIAEGVETKSQERFLAFEGCQQIQ
                     GYVLSKPLSAEDFAARFLVSRQTLGSNAQAPL"
     gene            complement(3900..5756)
                     /gene="rpoD"
                     /locus_tag="VIO83_00760"
     CDS             complement(3900..5756)
                     /gene="rpoD"
                     /locus_tag="VIO83_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY8329852.1"
                     /translation="MSVKAQQQSRLKELIARGREQGYLTYAEVNDHLPEDISDPEQVE
                     DIIRMINDMGINVFETAPDADALLLAEGGDTDEAAAEEAAAALAAVDSDIGRTTDPVR
                     MYMREMGTVELLTREGEIEIAKRIEEGIREVMSAIAHFPGSVESILAEYQRVTSEGGR
                     LSDILSGYIDPDDGTLPAESEAVPTPVAKEVAPAAETEEDDEEAKADTSEEEGDGGPD
                     PEEAARRFGAVAEQLEKARKALKKFGRGSPQGIAELTALAELFMPIKLVPKQFDVLVT
                     MVRDALDRVRQQERAIMQLCVRDARMPRADFLKLFPSNEINLSWSEGLSGGKAKYAEA
                     IGRVQADIQRNQQILIDLENAVGLTIAEIKDINRCMSVGEAKARRAKKEMVEANLRLV
                     ISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIAD
                     QARTIRIPVHMIETINKLNRISRQMLQEMGREPTPEELGERMEMPEDKIRKVLKIAKE
                     PISMETPIGDDEDSHLGDFIEDSSMQSPIDVATVESLKEATREVLAGLTAREAKVLRM
                     RFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPTRSEHLRSFLDE"
     gene            complement(5825..>6283)
                     /locus_tag="VIO83_00765"
     CDS             complement(5825..>6283)
                     /locus_tag="VIO83_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY8329853.1"
                     /translation="VMPPRGGAGVEPPTLSALRTLLHHPQLAQKVEDAGHFAAEDDTY
                     TQLLVALLESLQKHPQQNSLQLIARWHGTEQGRLLRGLAEKEWLIQQDNLEQQFFDTI
                     TTLASRQRERSLEGLLRKARHSELSAEEKLRLRELLSRSEAPASPGPSGA"
BASE COUNT          899 a         1935 c         2249 g         1200 t
ORIGIN      
        1 gccgcccgcg ggcggctttt tcatgtgcgg cgacggtccg gaactcagcg cggcgcgccg
       61 tcctgggcgt tggcgagcat ggcgctggcc agttcctcgt cgctggcctt gaggccggga
      121 tggtcgcggc ggatctgctg cagcgcggcc tccaggtagg cgccgcggat cgcaccgccg
      181 ctggcgacga agctggaggc gtcgtcctcg gcggcaagca ccagtttctc gtccttgaag
      241 gtggaataca gcgaggcgga aacccccgcg gaggtggcga catcctccca gtccacatcg
      301 gccgccagga tggcgccgac gggcaggaag aaggtcaagg ctgcgaccag cagtgtacgg
      361 cgcatcacgg taatcctcgg caagatacaa gcatcctgtt tccatgagag cggcgttggc
      421 ggtcgagagt tcccgcgggc aggtgccatc catcgcaggc ggacagccgc gagtcagggc
      481 gggtgcgtgc tgtcggcggc tctggcggga gaagtgcaaa agacaggcgg gcagcgcacg
      541 tgtcgcggct gccatgtgcg gcaatcgccg gggcggcgaa ggggaggtgg tgggcccagc
      601 aggacttgaa cctgcgacca agcgattatg agtcgcctgc tctgaccaac tgagctatag
      661 gcccgctatc gaaccccagg ttgtatgcct gcgggggtag ttccatgcgg cgaggccggc
      721 gggcgtcctc tccaggcttg ccgcaccatg aggggcggca aggcgccgcg attatagcgg
      781 cgcctgcgcg ttggagccaa gggtttgccg ggatacgaga aagcgtgccg cgaagtcctc
      841 ggccgacaac ggcttgctca gcacgtagcc ctggatctgc tggcagcctt cgaaggcaag
      901 gaagcgctcc tgcgacttgg tctccacccc ctcggcgatc accgtcagct gcatgctgtg
      961 gccgagggcg atgatggcgc gggtgatggc cgcgtcgttg ggatcgtccg gcaggccggc
     1021 gacgaacgac tggtcgatct tcagggtgtc gatgggcagg cgcttgagat agctcagcga
     1081 cgagtagccg gtgccgaagt cgtcgatcgc cagttgcagg ccgagctcct tgagcgcctg
     1141 gaggatcggc agggcctcct ccttctggct catgacgaag gtttcggtga tttccagctg
     1201 caggctggcg ggctccagtc cgtggctggc caacagttcg ctgatccgcc cgaccagttg
     1261 cggctggcgc agttgcgggc cggccaggtt gaccgccagt gcaccgaatg ccaggtattg
     1321 cgcctgccac acactcatct gccggcaggc ttcctgcaac acccagtcgc ccagctcgat
     1381 gatgctgccg ttttcctcgg cgaggggaat gaagcgctca ggcggcacgt cgccgtgcag
     1441 cggatgctgc cagcgcagca gcgcttcggc accgaccagc cggccgctgt tcaggcactg
     1501 cttgggctgg tagtgcaggt gcagctcgcc gcgctcggcc gcgcggcgca ggtcgttttc
     1561 cagggtcatg cgctcggtgg ccaggaagct caggtcggcg gtgtagaact cgatgcggtt
     1621 gcgcccgcgg gccttggcca ggtacatcgc gctgtcggcg ttcttcacca gggtggcgac
     1681 atcctggcca tgttcgggga acaggctgat gcccatgctc gagctcatga agaactcgtg
     1741 gccgtcggcg ttgaacgggg tgctgaaggc gctcatcagc ttgctggcga cccgctcgat
     1801 gtcgctggtc tgcgagacgc tgggcatcag cacgatgaat tcgtcgccgc cgaggcgggc
     1861 cacggtgtcg acctcgcgca gctgttcgcg cagacgcttg gcgatgccct gcagcagcag
     1921 gtcgccgacc ggatggccga ggctgtcatt gatgtgcttg aagcggtcga ggtcgaggaa
     1981 cagcacggcg ccgcggcgtt gttcggtctg cgcctcgtcg agggccagct gcaggcggtt
     2041 ctcgaacagc aggcggttgg gcaggccggt cagcgggtcg tggtgggcct ggtagtcgag
     2101 gcgcgcctgg gcgtgcttga ggctggtgat gtcggcgaac accgcgacat ggtgcgaaat
     2161 ccggttgccg ccgttgcgca cggcgctgat ggtcatccag gccggatagg actcgccgtt
     2221 cttgcgccgg ctccagatct cgccctgcca gtggccgtcg cgctcgaggc tgctgcggat
     2281 ctcctcctcg aaatcctgac catgctgtcc ggaggccagc agctgcgggg tctggccgag
     2341 ggcctcgctc tcgctgtagc cggtgatggc gctgaaggcg cggttgatgg cgacgatgcg
     2401 ctgctcgagg tcggtgatca tcactccttc ggctgtgctt tcgaacaccg tggcggcctg
     2461 ctgcaggcgc tcctgcatct gcatgcgctc gctgatatcg cgcgcgatga tcagtgcaca
     2521 gtcctcgccg tcgatctgca ggggttgggc gctgaactcg cacaggcgct cctggccgtc
     2581 gcgggtgcgt acccgggcgg acaggtgctg cgcgctgccg tcctgctcga gctggcgcat
     2641 gaggcgctgg cgatcggccg gatcgaccca caggcccagc tcgagggtgc tgtggttgat
     2701 ggcttcgctg gggctgtagc cggtgatctg ggtgaagccg gggttggcct cgagcagccg
     2761 gccgtcgcgc aggcgggtga tgctcagcgc atcgggcgag gcatagaagg ccttggcgaa
     2821 cttctcctcg ctggcgtgca gcagctggcg ggtgtggtgc agggcggaaa ggtcgcgcag
     2881 caccacgatg gcggccggct gcccgtccag ctcgacgcgc ctggccgaga gcaggccggt
     2941 gaagtcttcg ttgccgcggc ggatcagagt gacctcgaca ttctgcaggt cgtggctgtg
     3001 cagctggtgg agcacccggc ggccctgctc gtgctggcgc cagaggccca gttcgctggt
     3061 ggtgtgtccg caagcggtgt ccgccggcag gccggtggct tcctcgaagg cccggttggc
     3121 ggcgaggatg cgcccgctgt ggcgttcgat cagcagcatg atctcggggc agtcgtgaaa
     3181 cgccgagcgg tattgctgct cggactggcg cagcgcctgt tcgctcagcg ggttctcgcc
     3241 gatgtcgatg aacagcccct gcagcgtggc gtcgtcaccc gcgccacggc gctggacgat
     3301 agccagcagg cggatcaggc gaccgtcggc ggcgatcagc cggcattcgt ggcgcgcttg
     3361 cgcgggctcg ccttcgccgc gcagaaaggc gagggccgct ggagcgtccg gcggatacag
     3421 ggtgcggctg cagaagtccg cttgcagcca ggcgttgacg gggtagccga gcagcgcctc
     3481 gtccggcgcc gagatctggg tgaagcgcag gtcgtgccgg cacatttccc agatgaccaa
     3541 cggggcgcgc tccagcagtt cgcggtcgtg cgcggcgcgt gcctgcaggg cccgctgcag
     3601 acgctcgagc tgtcgtcgct gccggccacg cagcagcagg cccagggcca gcagggtcag
     3661 cagcagggct gcggcatagg gggcaaggag cagccacggg tcccgggtcc atcctggcag
     3721 ggcggcgtgg ctgtaggcgg ggagcacggc caggcagagt actggcagtg ccggcaggcg
     3781 cggcatggcg atccctcgtg tgcgcgttca tcgtcaaagt gtagaggctg tgccggtcct
     3841 gcgcgcaata aaaaaccccg gagccttgcg gcgccggggt ttttcgtctt gcggtgcgct
     3901 tactcgtcga ggaacgagcg caggtgttcg ctgcgggtcg ggtggcgcag cttgcgcagg
     3961 gccttggcct cgatctggcg gatgcgctcg cgggtgacat cgaactgctt gccgacttcc
     4021 tcgagggtgt ggtcggtgtt catgtcgatg ccgaagcgca tgcgcagcac cttggcttcc
     4081 cgggcggtga ggccggcgag gacttcgcgg gtggcttcct tgaggctttc cacggtcgcc
     4141 acgtcgatcg gcgactgcat gctggagtcc tcgatgaagt cgcccaggtg cgagtcttcg
     4201 tcgtcaccga tcggggtttc catggagatc ggctctttgg cgatcttcag caccttgcgg
     4261 atcttgtcct cgggcatctc catgcgctcg cccagctctt ccggagtcgg ctcgcggccc
     4321 atctcctgga gcatctgccg ggagatgcgg ttgagcttgt tgatcgtctc gatcatgtgc
     4381 accgggatgc ggatggtgcg cgcctggtcg gcgatcgagc gggtgatcgc ctggcggatc
     4441 caccaggtgg cgtaggtcga gaacttgtag ccgcggcggt attcgaactt gtccaccgcc
     4501 ttcatcaggc cgatgttgcc ttcctggatc aggtcgagga actgcaggcc gcggttggtg
     4561 tacttcttgg cgatggagat gaccaggcgc aggttggcct cgaccatttc cttcttcgcc
     4621 cggcgcgcct tggcctcgcc gaccgacatg cagcggttga tgtccttgat ctcggcgatg
     4681 gtcaggccga ccgcgttttc gagatcgatg aggatctgct ggttgcgctg gatgtcggcc
     4741 tgcacgcggc cgatggcctc ggcgtacttg gccttgccgc cgctcagacc ctcgctccag
     4801 ctcaggttga tctcgttgct ggggaacagc ttgaggaagt cggcacgcgg catgcgagcg
     4861 tcacgcacgc acagctgcat gatggcgcgt tcctgctgac gcacgcggtc gagggcgtcg
     4921 cggaccatgg tcacgagcac gtcgaactgc ttgggcacca gcttgatcgg catgaaaagc
     4981 tcggccagcg cggtcagctc ggcgatgccc tgcgggctgc cgcggccaaa cttcttcagc
     5041 gccttgcggg ccttttccag ctgctcggcc acggcgccga agcggcgggc ggcttcttcc
     5101 ggatcgggac cgccatcgcc ttcttcttcg ctggtgtcgg ccttggcttc ctcgtcgtcc
     5161 tcttcggtct ccgccgccgg cgcgacttcc ttggcgaccg gggtcggcac ggcttcgctt
     5221 tccgccggca gggtgccgtc gtcggggtcg atgtagccgc tgaggatgtc ggacagacgg
     5281 ccaccttcgc tggtgacccg ctggtattcg gcgaggatgc tttccaccga gccggggaaa
     5341 tgggcgatgg cgctcatcac ctcacggatc ccttcctcga tgcgcttggc gatctcgatc
     5401 tcgccttcac gggtcagcag ctccacggtg cccatttcgc gcatgtacat gcgcaccggg
     5461 tcggtggtgc ggccgatgtc gctgtccacg gcggcgaggg cggcggcggc ttcctcggcg
     5521 gcagcctcgt cggtatctcc cccctcggcg agcagcaggg catccgcatc cggagctgtc
     5581 tcgaatacgt tgatccccat gtcgttgatc atgcggatga tgtcttccac ctgctccgga
     5641 tcggaaatat cttccggcag gtggtcgttg acctcggcgt aagtcaggta accctgctca
     5701 cggccacggg cgatcaactc tttgagacgg gactgctgtt gcgctttaac ggacatgttc
     5761 accctatcca ctgaaggtct tggcgggctg aaaagaagcc gagcattata ctcgatccgg
     5821 gggcttacgc accagatggg cccggggatg cgggggcttc gctacggctc agaagctcgc
     5881 gcaggcggag tttttcctcc gcgctgagtt cgctgtgccg cgctttgcgc aacaggcctt
     5941 cgaggctgcg ctcgcgctga cggcttgcca aggtagttat agtgtcgaaa aactgttgtt
     6001 caaggttgtc ctgctgaatc agccattcct tttccgctag accgcgcaac aggcgtcctt
     6061 gttccgtgcc gtgccagcgg gcgatcagct gcagcgagtt ctgctgcggg tgtttctgca
     6121 gcgattcgag cagggccacc agcagctggg tgtaggtatc gtcttcggct gcgaagtggc
     6181 cggcatcctc gaccttctgc gccagttgcg ggtggtgcag cagggtgcgc agggcgctca
     6241 gggtcggcgg ttcgacgccg gcaccgccgc gcggcggcat gac
//
LOCUS       DATCUN010000024         7014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_94862, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000024 DATCUN010000000
VERSION     DATCUN010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94862"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VIO83_00770"
     CDS             <1..138
                     /locus_tag="VIO83_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8329854.1"
                     /translation="RFHMHTSLLEGVNNRIKVIKRMAYGFRDVDYFFLKIKAAFPGKM
                     R"
     gene            complement(191..778)
                     /locus_tag="VIO83_00775"
     CDS             complement(191..778)
                     /locus_tag="VIO83_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajG family lipoprotein"
                     /protein_id="HEY8329855.1"
                     /translation="MLPRLLFGLIAAASLTLVGCAHSPQQLTVQPVVTAQLAPVGQGQ
                     PVVVRVVDGRPSPVLGTRGGLYPETSAISVSSQSLVPRLQGQAEAAVRLLGFTPTPNA
                     YNAPQLTLTVTDMKYVSPKDGAYVTEANISASIRADVQNSQRRYSGRYGSSLNQRFGM
                     APNQQTNSKLVSDVLSDALTRVFKDPTIGQLLNNQ"
     gene            complement(942..2036)
                     /gene="zapE"
                     /locus_tag="VIO83_00780"
     CDS             complement(942..2036)
                     /gene="zapE"
                     /locus_tag="VIO83_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEY8329856.1"
                     /translation="MTPLQRYQADLQRPDFFHDAAQENAVRHLQRLYDDLVAGQQDPG
                     LLGKLFGRKKPQGPIKGVYFWGGVGRGKTYLVDTFFDALPFKQKMRTHFHRFMKRVHE
                     EMRTLKGEKNPLTIIGKRFADEARVICFDEFFVSDITDAMILATLLEELFKNGVSLVA
                     TSNIVPDGLYKDGLQRARFLPAIALLKQHTEIVNVDSGIDYRLRALEQAELYHFPLDA
                     EAELSLDKSFRSLLTENCVVQDNEALLIENREIRARKVANDVAWFEFRELCDGPRSQN
                     DYIELGKIFEAVLISNVEQMGVSKDDMARRFINLVDEFYDRNVKLILSAEVELKDLYT
                     GGRLEFEFQRTLSRLLEMQSHEFLSRPHKP"
     gene            complement(2250..3626)
                     /locus_tag="VIO83_00785"
     CDS             complement(2250..3626)
                     /locus_tag="VIO83_00785"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY8329857.1"
                     /translation="MTTRILTGITTTGTPHLGNYAGAIRPAIVASRADNVDSFYFLAD
                     YHALIKCDDPLRIQRSRLEIAATWLACGLNPERVTFYRQSDIPEIPELTWLLTCVAAK
                     GLLNRAHAYKASVDQNVAKGEDPDAGVTMGLFSYPVLMAADILMFNAHQVPVGRDQIQ
                     HVEMARDIGQRFNHLFGQGREFFALPEALIEESVATLPGLDGRKMSKSYDNTIPLFGS
                     SKQLKDAVARIVTDSKLPGEPKDADNSHLFTLYQAFASAGQTTEFRAALEGGLAWGEA
                     KQRLSELLEGELGEARERYNALIAKPAELEDILLAGAAKARKIATPFLGELREAVGLR
                     SFKERLVSKGGEKERAKGKKPKFLSFKDDDGLFRFKFVTVDGEQILLSEGFDNPRDAG
                     EAIKALSGFSGDRLPLFDSETETGTLIFEVCNNGRTVAVSYEFSSEKERDAAKAVLRA
                     AFDQLHLG"
     gene            complement(3650..4285)
                     /locus_tag="VIO83_00790"
     CDS             complement(3650..4285)
                     /locus_tag="VIO83_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8329858.1"
                     /translation="MSLTETPQLLDGPCGALEALYLDRADARGLALICHPHPLFAGSM
                     HNKVVATLQRAARDAGYATLRFNFRGVGHSAGEHAHGEGEIDDAEAAARWLLAQHPGL
                     PLTLLGFSFGSCVAACLGGRLEQQGVEVERLFMLAPPVERFSVEGRLPQRCPLTVIQP
                     EDDEVVTPTAVYAWSATLQRAHELLKVAECGHFFHGKLTELKALVLPRLQS"
     gene            4398..4823
                     /locus_tag="VIO83_00795"
     CDS             4398..4823
                     /locus_tag="VIO83_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HEY8329859.1"
                     /translation="MEQSFTAWLLPALALIAGVGIGFLLARLLPGTAPTQVQHQLDEM
                     QERFEAYQSEVVSHFNTSASLMQKVAQNYQDIQDHLAEGANRLALDELSRQRLLAALS
                     DSAQPARERITAPASTEPPKDYAPGSSGALSDEEVLKKA"
     gene            complement(4978..6879)
                     /gene="cysN"
                     /locus_tag="VIO83_00800"
     CDS             complement(4978..6879)
                     /gene="cysN"
                     /locus_tag="VIO83_00800"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEY8329860.1"
                     /translation="MSHQSELISEDILAYLAQHERKELLRFLTCGNVDDGKSTLIGRL
                     LHDSKMIYEDHLEAITRDSKKVGTTGEEVDLALLVDGLQAEREQGITIDVAYRYFSTA
                     KRKFIIADTPGHEQYTRNMATGASTCDLAIILIDARYGVQTQTRRHSFIASLLGIKHI
                     VVAINKMDLMDFDQGVFDSISADYLQFAERIGLKPSSLHFVPMSALKGDNVVNKCERA
                     PWYQGPTLMEILETVEIAADRNLTDLRFPVQYVNRPNLNFRGFAGTLASGIVHKGDEV
                     AVLPSGKTSKVRSIVTFDGELEQAIPGQAVTLTLEDEIDVSRGDMLVHADNRPQVTDS
                     FDAMLVWMAEEPMLPGKKYDIKRATSYVPGNIVGIAHRVDVNSLEQLAASELKLNEIG
                     KVRVSLDAPIALDGYAHNRTTGAFIVIDRLTNGTVGAGMIIADPLAGRNTIGHHGQLA
                     HVAAEERAARFGQQPATVLFSGLSGAGKSTLAYAVERKLFDSGRAVYVLDGQNLRQDL
                     NKGLPLDRAGRAENWRRAAHVARQFNEAGLLTLAAFVAPDAEGREQARAVIGGERLIT
                     VYVQASPQACRERDPQGLYAAGEDNIPGESFAYDVPLDADLVIDTQSASVEEGVKQVL
                     ALLRSRGAI"
     gene            complement(6892..>7014)
                     /locus_tag="VIO83_00805"
     CDS             complement(6892..>7014)
                     /locus_tag="VIO83_00805"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242927.1"
                     /note="with CysN catalyzes the formation of
                     adenylylsulfate from sulfate and ATP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase small subunit"
                     /protein_id="HEY8329861.1"
                     /translation="ASLPEIIQEMLLTRTSERQGRVIDHDGAGSMEEKKRQGYF"
BASE COUNT         1177 a         2254 c         2327 g         1256 t
ORIGIN      
        1 cgattccaca tgcacaccag cctgctggag ggggtgaaca accggatcaa ggtgatcaag
       61 cgcatggcct atggcttcag ggacgtggac tacttcttcc tgaagatcaa ggctgccttt
      121 cccgggaaaa tgcgatgaac cgaaaaaaag gcggtggggg caccaccgcc tttggcgaac
      181 cgcgacgcgc ttactgattg ttcagcagct ggccaatggt cggatccttg aacacccgag
      241 tcagcgcatc gctgagcaca tcgctgacca gcttgctgtt ggtctgctgg ttgggcgcca
      301 tgccgaagcg ctgattcagc gaggaaccat agcgaccgct gtagcggcgc tggctgttct
      361 gcacgtcagc acggatgctg gcgctgatgt tcgcctcggt gacataggcg ccgtccttgg
      421 gcgacacgta cttcatgtcg gtcaccgtca gggtcagttg cggcgcattg taggcattcg
      481 gcgtcggcgt gaagccgagc aggcgcacgg ccgcctcggc ctggccttgc aggcggggca
      541 ccaggctctg actgctcacg ctgatcgcgc tggtttccgg atagaggccg ccgcgcgtgc
      601 cgagtaccgg cgagggccgg ccgtcgacca cccgcaccac caccggctgc ccctgaccga
      661 ccggcgcgag ctgcgcggtc accacgggtt gcacggtgag ttgttgcggg ctgtgggcgc
      721 aacccaccag ggtcagactg gcggcagcga tcaggccaaa cagcagacgg ggcagcatgt
      781 tcatctctcc gggggactgg atagggaaga caggaaacgg ccggcagtat acccagccgc
      841 gccgagcacc gcgacaagcg gcggcatgcc cttgcgactg ccggcggcgc cgacagttcc
      901 ccacaaacga caagggcgcc cgatcgggcg cccctgcagg attacggctt gtgcggccga
      961 ctgaggaatt cgtgggactg catctccagc aggcggctca gagtgcgctg gaactcgaac
     1021 tccagacgcc cgccggtgta caggtccttg agttccacct cggcggagag gatcagcttg
     1081 acgttgcgat cgtagaactc gtccaccagg ttgatgaagc gccgcgccat gtcgtccttg
     1141 ctcacgccca tctgctcgac gttggagatc agcaccgcct cgaatatctt gcccagctcg
     1201 atgtagtcgt tctggctgcg cgggccgtcg cacagctcgc ggaactcgaa ccaggccacg
     1261 tcgttggcca ccttgcgcgc gcggatctcg cggttctcga tcagcagcgc ctcgttgtcc
     1321 tgcaccacgc agttttccgt gagcaggctg cggaagctct tgtccaggct cagctcggcc
     1381 tcggcatcca gcgggaagtg gtacagctcg gcctgctcca gcgcccgcag gcggtagtcg
     1441 atgccgctgt cgacgttgac gatctcggtg tgctgcttga gcagggcgat cgccggcagg
     1501 aagcgcgcgc gctgcaggcc gtccttgtac aggccgtccg gcacgatgtt ggaagtcgcc
     1561 accaggctga cgccgttctt gaacagctcc tcgagcaggg tggcgaggat catcgcgtcg
     1621 gtgatgtcgg agacgaagaa ctcgtcgaag cagatcaccc gcgcctcgtc ggcgaaacgc
     1681 ttgccgatga tggtcagcgg attcttctcg cccttgaggg tccgcatttc ctcgtgcacg
     1741 cgcttcatga agcggtggaa gtgggtgcgc atcttctgct tgaacggcag tgcgtcgaag
     1801 aaggtatcga ccagataggt cttgccgcgg cccacgccgc cccagaagta gacgcccttg
     1861 atcggcccct gcggtttctt gcggccgaac agcttgccca gcaggcctgg atcctgctgc
     1921 ccggcgacca ggtcgtcgta gagacgctgc agatgacgca cggcattttc ctgcgcggca
     1981 tcgtggaaga aatcggggcg ttgcagatcg gcctggtagc gctggagcgg agtcatggcg
     2041 gcgtcggggt tggaaaacgg ggccgtcact ttaacggcgc cctcgccgct tggcaattta
     2101 cgcagatcgt tcaacacgta cctccctcat caaggggtcg caaggttcgg gaccgcttcc
     2161 ctgtccccgc ggcccggcgg ccgcaacccc gccgccgggc cgtcctcagg cttcctgggg
     2221 tgccagcgcc tcggccaggc gcgccatctt caccccaagt gaagctgatc aaatgctgcc
     2281 ctaaggactg cttttgccgc atctctttct ttctcggacg aaaactcata gctaactgct
     2341 accgtgcgcc cgttgttgca gacttcaaat attaaagtcc ctgtctccgt ctcagaatcg
     2401 aagagaggga gacgatcccc actaaaaccc gacagagcct ttatcgcctc tcccgcatcg
     2461 cgtgggttat cgaagccctc tgaaagaaga atctgctcac cgtccacagt cacaaacttg
     2521 aatctaaaaa gaccatcatc atctttaaag ctgaggaatt ttggtttctt ccctttggct
     2581 cgctccttct ctcctccctt gcttaccaag cgctccttaa acgagcgcag ccccaccgcc
     2641 tcgcgcagct cgccgaggaa cggcgtggcg atcttgcgcg ccttggcggc accggcgagg
     2701 aggatgtcct cgagttcggc cggcttggcg atcagcgcgt tgtagcgctc gcgcgcctcg
     2761 cccagctcgc cctccagcaa ctcggacagg cgctgcttgg cttcgcccca ggccaggccg
     2821 ccctccagcg ccgcgcggaa ctcggtcgtc tgcccggcac tggcaaaggc ctggtacagg
     2881 gtgaacaggt gcgagttgtc ggcatccttc ggctcgcccg gcagcttcga gtcggtgacg
     2941 atgcgcgcca ccgcgtcctt gagctgcttg cttgagccga acagcggaat ggtgttgtcg
     3001 tagctcttcg acatcttgcg cccgtcgagg cccggcaggg tcgccacgct ctcctcgatc
     3061 agcgcttccg gcagggcgaa aaactcgcgg ccctggccga acagatggtt gaagcgctgg
     3121 ccgatgtcgc gcgccatctc cacgtgctgg atctggtcgc gaccgaccgg cacctggtgg
     3181 gcgttgaaca tcaggatgtc ggcggccatc agcaccggat agctgaacag gcccatggtc
     3241 accccggcat ccgggtcctc gcccttggcc acgttctggt cgaccgaggc cttgtaggca
     3301 tgggcacggt tgagcaggcc cttagccgcc acgcaggtga gcagccaggt cagctcgggg
     3361 atctcgggga tatccgactg gcgatagaag gtcacccgct cggggttgag accgcaggcc
     3421 agccaggtcg cggcgatttc caggcgcgag cgctggatgc gcagcgggtc gtcgcacttg
     3481 atcagcgcgt ggtagtcggc gaggaagtaa aaggagtcca cgttgtccgc gcgactggcg
     3541 acgatcgccg gacggatggc gccggcatag ttgcccaggt gcggcgtgcc ggtggtggtg
     3601 ataccggtga gaatacgggt ggtcatgatc gatcgcttgt cggactgcat caggattgga
     3661 gacgcggcag caccagcgct ttcagctcgg tcagcttgcc gtgaaagaag tggccgcatt
     3721 ctgccacttt gagcagctca tgggcacgct gcagtgtcgc cgaccaggca tacaccgccg
     3781 tcggcgtgac cacctcgtcg tcttccggct ggatcaccgt caacgggcag cgctgtggca
     3841 ggcgaccctc cacgctgaaa cgctccaccg gcggggccag catgaacagg cgctcgacct
     3901 cgacgccctg ttgttccaga cgcccgccca ggcaggcggc cacgcaggag ccgaaggaga
     3961 agccgagcag ggtcagcggc aggccggggt gctgcgccag cagccagcgc gcggcagctt
     4021 cggcgtcgtc gatctcgcct tcgccatggg cgtgctcgcc agcgctgtgg ccgacgccgc
     4081 gaaagttgaa gcgcagggtg gcgtagccgg catcgcgcgc ggcgcgctgc agggtggcga
     4141 ccaccttatt gtgcatgctg ccggcgaaca gcggatgggg atggcagatc agcgccaggc
     4201 cacgggcgtc ggcgcgatcc agatacaatg cctcgagcgc gccgcagggg ccgtcgagca
     4261 gctggggggt ttcagtcaat gacacaggaa ctccgtgacc ccggcaaggg gcaactcgtc
     4321 tagcacactg ccagagctat acagcgccgg cctgacccgt taacgtaatg caaatcccgt
     4381 gtatagagga aggattcgtg gaacagtcat tcactgcctg gctgctgcca gccctggccc
     4441 tgattgccgg cgtcggcatc ggtttcctgc tggcccgcct gctgccgggc accgccccga
     4501 cccaggtcca gcaccagctg gacgagatgc aggagcgctt cgaggcctac cagagcgaag
     4561 tggtcagcca cttcaatacc tccgccagcc tgatgcagaa ggtggcgcag aactatcagg
     4621 acattcagga tcacctcgcc gaaggtgcca accgcctggc gctggacgaa ctgtcccgcc
     4681 agcgcctgct cgccgccctg agcgacagtg cccagccggc ccgcgagcgc atcaccgcgc
     4741 cggccagcac cgagccgccg aaggactacg ccccgggcag ctcgggcgcc ctgagcgacg
     4801 aggaagtcct gaagaaggcc tgatccgtca ggtcagcgaa caagccccgc cccgcgcggg
     4861 gcttcttttt gtccgcaagg cgccggctgg cgatctcgcg ccacgcgctg ccaccacccc
     4921 acaaacgaca aggccccggc atcgctgccg gggccttggc atgtcaggcg ggacggatca
     4981 gatcgcgccg cgcgaacgca gcagcgccag tacctgcttg acaccctcct ccaccgaggc
     5041 ggactgggta tcgatcacca gatcggcatc cagcggcacg tcgtaggcga aggactcgcc
     5101 ggggatgttg tcctcgccgg cggcgtacag gccctgcgga tcgcgctcgc ggcaggcctg
     5161 cggcgaagcc tgcacgtaga cggtgatcag tcgctcgccg ccgatgaccg ccctggcctg
     5221 ctcgcgaccc tcggcatccg gggcgacgaa ggcggccagg gtcagcaggc cggcctcgtt
     5281 gaactggcgg gcgacatgcg cggcccggcg ccagttctcg gcacgcccgg cgcgatccag
     5341 cggcaggccc ttgttgaggt cctggcgcag gttctggccg tccagcacgt agaccgcgcg
     5401 accactgtcg aacagcttgc gctccacggc gtaggccagg gtgctcttgc cggcgccgga
     5461 caggccgctg aacagcacgg tggccggctg ctggccgaag cgcgcggcgc gctcctcggc
     5521 agccacgtgg gccagctggc cgtggtgacc gatggtgttc cggcccgcca gcggatcggc
     5581 gatgatcatg ccggcaccga cggtgccgtt ggtcaggcgg tcgatgacga tgaaggcgcc
     5641 ggtggtgcgg ttgtgcgcgt agccgtcgag ggcgatgggc gcatccaggc tgacgcgcac
     5701 cttgccgatc tcgttgagct tgagttcgct ggcggccagc tgctcgaggc tgttgacgtc
     5761 gacgcggtgg gcgatgccga cgatgttgcc cggcacgtag ctggtggcgc gcttgatgtc
     5821 gtacttcttg cccggcagca tcggctcctc ggccatccac accagcatgg cgtcgaagct
     5881 gtcggtgacc tgcggacggt tgtcggcgtg caccagcatg tcgccgcgcg acacgtcgat
     5941 ctcgtcttcc agggtcaggg tcaccgcctg gccgggaatc gcctgctcca gctcgccgtc
     6001 gaaggtgacg atggagcgca ccttgctggt cttgcccgac ggcagcacgg cgacttcgtc
     6061 gcccttgtgc acgatgccgc tggccagggt gccggcgaag ccgcggaagt tcaggttcgg
     6121 ccggttgacg tactgcaccg ggaagcgcag gtcggtcagg ttgcggtcgg cggcgatctc
     6181 gacggtctcg aggatctcca tcagcgtcgg cccctggtac cagggcgcac gctcgcactt
     6241 gttgaccacg ttgtcgccct tcagcgccga catcggcacg aagtgcagcg agctgggctt
     6301 gagaccgatg cgctcggcga actgcaggta gtcggcactg atgctgtcga acacgccctg
     6361 atcgaagtcc atcaggtcca tcttgttgat ggcgacgacg atgtgcttga tgcccagcaa
     6421 ggaggcgatg aagctgtgcc ggcgggtctg ggtctgcacg ccgtagcggg cgtcgatcag
     6481 gatgatcgcc aggtcgcagg tcgacgcgcc ggtggccatg ttgcgggtgt actgctcatg
     6541 gccgggggtg tcggcgatga tgaacttacg cttggcggtc gagaagtagc ggtaggccac
     6601 gtcgatggtg atgccctgct cgcgctcggc ctgcaggccg tcgaccagca gggccagatc
     6661 gacctcctcg ccggtggtgc cgactttctt cgagtcgcgg gtgatcgctt cgaggtggtc
     6721 ctcgtagatc atcttggagt cgtgcagcag gcgcccgatc agggtgctct tgccgtcgtc
     6781 gacgttgccg caggttagga agcgcagcag ttccttgcgt tcgtgctggg ccaggtaggc
     6841 gaggatgtcc tcgctgatca gttcggattg atgcgacatg atgcggaaac cttagaagta
     6901 gccctgacgt ttcttttctt ccatggaacc ggcgccatcg tggtcgatga cgcggccctg
     6961 gcgctcggaa gtgcgggtca ggagcatttc ctggatgatc tcgggcaggc tggc
//
LOCUS       DATCUN010000025         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_263680, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000025 DATCUN010000000
VERSION     DATCUN010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263680"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /gene="tssC"
                     /locus_tag="VIO83_00810"
     CDS             <1..1302
                     /gene="tssC"
                     /locus_tag="VIO83_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEY8329862.1"
                     /translation="VKKAMVDRMIAEIDAKLSRQMDEILHHPQFQSLESSWRGLKLLV
                     DRTDFRENIKLELLNASKQDLLDDFEDSPEIFQSGLYKHVYTAEYGQFGGQPVGALIA
                     NYFFDPSAPDVKTLQYVASVASMAHAPFIAAAGPKFFGLESFTGLPDLKDLKDHFDGP
                     QFAKWQSFREQEEARYVGLTVPRFLLRTPYDPQENPVKTFVYQESVSASHEHYLWGNT
                     AYTFASRLSDSFAKFRWCPNIIGPQSGGAVEDLPLHHFASMGEIETKIPTEVLVSDRR
                     EYELAEEGFIALTMRKGSDNAAFFSANSVQKPKHFGISAEGKAAELNYKLGTQLPYLF
                     VVNRLAHYLKVMQREQIGSWKERSDLERELNKWIRQYVADQDNPSAEVRGRRPLRAAQ
                     IAVSEVDGEPGWYRVSLSVRPHFKYMGADFTLSLVGKLDKE"
     gene            1309..1713
                     /gene="tssE"
                     /locus_tag="VIO83_00815"
     CDS             1309..1713
                     /gene="tssE"
                     /locus_tag="VIO83_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HEY8329863.1"
                     /translation="MAYGSLFERLGGEAGQRNGWSLEVAAMASVAAHLAKMLSTRAGS
                     VQTLPDYGLPDLNDMRLSLHDSLQQARAAIERFIEAYEPRLRRVRVVSLPRDHDPLTL
                     AFAIEGWLEVDGFSRQVSFAARLDGSGQVKVS"
     gene            1725..2012
                     /locus_tag="VIO83_00820"
                     /pseudo
     CDS             1725..2012
                     /locus_tag="VIO83_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252593.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
BASE COUNT          385 a          755 c          694 g          327 t
ORIGIN      
        1 gtgaagaagg ccatggtcga ccgcatgatc gcggagatcg acgccaagct cagccggcag
       61 atggacgaga tcctccacca cccgcagttc cagtcgctgg agtcgtcctg gcgcggcctc
      121 aagctgctgg tcgaccgcac cgacttccgc gagaacatca agctcgagct gctcaatgcc
      181 tccaagcagg acctgctcga cgacttcgag gacagcccgg agatcttcca gtccggcctg
      241 tacaagcacg tctacaccgc cgagtacggc cagttcggcg gccagccggt cggcgcgctg
      301 atcgccaact acttcttcga ccccagcgcg ccggacgtca agaccctgca gtacgtcgcc
      361 agcgtcgcca gcatggccca cgcgccgttc atcgccgccg ccgggccgaa gttcttcggc
      421 ctggaaagct tcaccggcct gcccgatctc aaggacctca aggaccactt cgacggcccg
      481 cagttcgcca agtggcagag cttccgcgag caggaagagg cccgctacgt cggcctgacc
      541 gtgccgcgct tcctgctgcg cacgccgtac gatccgcaag agaacccggt gaagaccttc
      601 gtctaccagg aaagcgtcag cgccagccac gagcactacc tgtggggcaa caccgcctac
      661 accttcgcca gccgcctgtc cgacagcttc gccaagttcc gctggtgccc gaacatcatc
      721 ggcccgcaga gcggcggcgc ggtggaagac ctaccgctgc accacttcgc cagcatgggc
      781 gagatcgaga ccaagatccc caccgaggtg ctggtgtccg atcgccgcga gtacgagctg
      841 gccgaggaag gcttcatcgc cctgaccatg cgcaagggca gcgacaacgc cgcgttcttc
      901 tccgccaact cggtgcagaa gccgaagcat ttcggcatca gcgcggaagg caaggccgcc
      961 gaactgaact acaagctcgg cacccagctg ccctacctgt tcgtggtcaa ccgcctggcc
     1021 cactacctca aggtcatgca gcgcgagcag atcggctcct ggaaggagcg cagcgacctc
     1081 gagcgcgagc tgaacaagtg gatccgccag tacgtcgccg accaggacaa ccccagtgcc
     1141 gaagtgcgcg gccgtcgtcc gctgcgcgcc gcgcagatcg ccgtcagcga ggtcgacggc
     1201 gagccgggct ggtaccgcgt cagcctcagc gtgcgcccgc acttcaagta catgggggcc
     1261 gacttcaccc tgtcgctggt cggcaagctg gacaaggagt aagcggccat ggcctacggc
     1321 agcctgttcg aacgcctcgg cggcgaggcc ggccagcgca acggctggag cctggaagtc
     1381 gccgccatgg cctcggtggc tgcccacctg gcgaagatgc tcagcacccg ggcgggcagc
     1441 gtgcagacgc tgcccgacta cgggttgccc gatctcaacg acatgcgcct gtcgctgcac
     1501 gattcgctgc agcaggcgcg tgccgccatc gagcgcttca tcgaggccta cgagccccgc
     1561 ctgcgccggg tgcgggtggt ttccctgccc cgcgaccacg atccgctgac cctcgccttc
     1621 gccatcgagg gctggctgga agtggacggc ttcagtcgcc aggtcagctt cgccgcgcgc
     1681 ctggacggta gcggccaggt caaagtcagc taaggagaac aaggatgtcc ggcaagcccg
     1741 ccgcccgcct gggcgatccc accgtctgcc cgctcccggg ccacggcaac aacccgatcg
     1801 ccgccggctc gcccgacgtg ctgttcgacg gcctgcccgc cgcccgccag ggcgatgcct
     1861 ccgcctgcgg cggcgccatg agcggcaacg tgattgccaa cgtgctgatc aacggccagc
     1921 cggcggcggt gctggggtcg gtgggtagcc atgggaatgt ggtggtgggg ggatcgggga
     1981 cggtgctgat cgggaccagt gggggtgggg ccgaatttat gggtattcgg gaaatcgtgt
     2041 tgatgaggct ggaatcaggt atgcgcaatg agcgtggcga tcttcagccg gaacaactcc
     2101 cacaggcctg ggtgcatgcg cctgtcaccg gcttcccact gctgccacgt tcgcaggccg
     2161 c
//
LOCUS       DATCUN010000026        24784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_57173, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000026 DATCUN010000000
VERSION     DATCUN010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 24784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24784
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57173"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2067
                     /locus_tag="VIO83_00825"
     CDS             <1..2067
                     /locus_tag="VIO83_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8329864.1"
                     /translation="VIKVLEEYRVPIDCIAGTSMGALVGASYATGTSVAEMETILKGI
                     STELLFKEAPPRQERAMRRKEDDYGILFTPEVGLDNGEVKFGKGIVSGVQLETVLRRL
                     SKAKGHYQFDKLPIPYRAVATDLVTGKAVVFSEGELANVMRASMSVPGAVAPAEVDGM
                     ILVDGMLTSNLPVQTTRNDMGADIIIAVNVGTPLLKREQLTGIFGVSGQMLSILTEQN
                     VQASLATLTPSDVLISPDLGDFSTGDFDHLEQIAPIGEAAARKVAGRLAQLSIPAGEY
                     AALRQRQVITVVPDLRPVDEIRVENLKRVNPKTVQASMATKAGEPIDQTTLDRDMRRI
                     YGTDDFEHVNYRFIEEPGKRILAVDAVEKTWGPNYLRFGLGLSSDFSGDAFFNILGSY
                     RKTWLNDRGAEWRTDVQLGHTSGLYTELYQPFSAESRFFVAPHAAIERRTSDLYRDDD
                     RVASYEMTSMLAGVDLGSQFNRYGELRLGMVGGRLKPQLDTGPESLSPGESRIMQGAF
                     TGQLRLDQLDSVHFPRSGWRAGANIFDSTDALGADDDYTKWDGDATAVYSFGNHTLNF
                     SIKVGDKLGGDPLPRYDQFQWGGFLTGSGYATGQLLGEELKYGRVMYYHRILEGSIFE
                     GAYTGFTLEATRIGNPLVPGNTDDWLRSAAIFIAADSPLGPVYLGYGQAEDGNSSFYF
                     YLGRPF"
     gene            2147..3553
                     /locus_tag="VIO83_00830"
     CDS             2147..3553
                     /locus_tag="VIO83_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEY8329865.1"
                     /translation="MGSAKNSTSKTKTKAAEAAPIFRSRDERIAAGRALREKLPRSDH
                     AAWTPPAKHRDPIAILEKSNKDRLPELVPIRYGRMLRSPFTFLRGSAALMAHDLTTMP
                     STGIRVQVCGDCHLMNFGLFATPERNLVFDLNDFDETLPAPWEWDIKRLAASFAVAGR
                     DNRLSNGESRDAAVACVLAYRENLREYSKRNPLEVWYSRLDEETLIETAPDEKVRKLR
                     QQITDKARERVVENAFPKLAEQVAGSPRIVDQPPIIYHVADPEFMDKVHEGLAEYRAT
                     LSDERRVLLDRYRLVDAAMKVVGIGSVGTRCFITLMLSEENHPLILQFKEACHSVLEP
                     YAGESRYDNQGQRVVMGQRLMQSSSDIFLGWARGRTGRDFFVRQLRDMKMSVPVEGVS
                     AAQLKRYAGLCGLVLARAHAKSGDAATISGYLGKGDTFDEALGDFSLAYADQTEQDHA
                     ILVEAVNSGRVEALIEEE"
     gene            complement(3587..4618)
                     /locus_tag="VIO83_00835"
     CDS             complement(3587..4618)
                     /locus_tag="VIO83_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8329866.1"
                     /translation="MRRKGTLLVLLILLLLPGLGAVSANPRSAERSPLPMTIGFQANT
                     DWLLLVARDLQLFEKAGLTPNFVKFVAGPPMIAAAQEQSIDVASIGSVPFLNGLTEGV
                     DWVVVGINPENAYGEGLVARKDSGIATVADLAGKRIGFFRGSTAHFGLMMALRQLGIR
                     RDQVELFEMAPAEQLSALASKQIDAAMVWEPWMQKMVHEADGRLIVTEGAMGIYTSVS
                     VYAARREWLRNNREAAVRFLRALLMAREVMQKSPEIGIKALAREMSIKEAWAEAIYEN
                     SPPPDLTLWVDPRYRYSLVKGAEFHRRLGYLVTFLLEEEVISEEVDVRNILDVTVISE
                     ALKARDAGR"
     gene            complement(4642..5712)
                     /locus_tag="VIO83_00840"
     CDS             complement(4642..5712)
                     /locus_tag="VIO83_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329867.1"
                     /translation="MTLGVLAICTLFCGCASIGPGTVARDRLDYSEAVGDSWKSQMLL
                     NLVKLRYGDAPVFLDVGQIVAGYSFPRSFGASAEVNQTDGTPAITSSVGLAAGATFND
                     SPTVTYTPMSGERFARSLMTPIPPSVILNIIQAGNPVEEVFRTSVQSVNGIDNRRVGR
                     LFVRRANPEFYALLPNLERLQSSGDIGMRLQQQGSETKAQMIFRPKTAAAEESARRNV
                     AQLLGLDPAAREYQVTYGTVASNDREIALLTRSIYEILVDIAAYISVPDAHVAEKRVR
                     PTAEGDIGPSGAIPPLIQVATSAQPPSDAFVAVPYQGYWFYIDNRDIPSKQVFSSIMF
                     LFTFVETRDHEAAPILTIPTTR"
     gene            complement(5747..7087)
                     /locus_tag="VIO83_00845"
     CDS             complement(5747..7087)
                     /locus_tag="VIO83_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY8329868.1"
                     /translation="MTDLQIYLTLGVFSAVILAIAFDLLDMAVAALLGVGLLLALGIL
                     TDNDLVAALRIAGGPISLLFGGMVVARVLASTGLFDRIGDIYLHATGGSGKRFLILLV
                     LLVAPVCAFLPNATTVILLAPVIIRVARALKVDIVGPMVITAIVSNAAGMLTLVGDPA
                     TFLVGSSIGMSFVQYLQTVSLAGLLAVLVVVPLLPWLMPQVWKARVELPTARQPVPLE
                     RKGFALLALGVLALMVVLFLFGEQLPVRIVPPSVAIIAASLALLVVYAVHVEPVEAVL
                     RDIDWKTLVFLGAIFCLVQAFTQTGLLQGLSLKLYEWFGVEFTLVALALLAGIGLLSS
                     LLANIPVVAAALVMTKGYLVAAEAVPEIALGAGFTDWPAATLPVFIALMFGATLGGNA
                     TLIGASANIVTVGICAGQGEKVTFARFSRYGVPITLVQLAISALYVVALFWLAH"
     gene            complement(7103..8011)
                     /locus_tag="VIO83_00850"
     CDS             complement(7103..8011)
                     /locus_tag="VIO83_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8329869.1"
                     /translation="MSEGIPGFSEQDGSGRWRGLDADFCRAVAAALLGDAEKVKFVPL
                     RSSTRFPALQARKIDLLVRNTTWTLTREALLQVQFPAVLFYDGQAFMVAKSSGITRLA
                     DFREGTVCVGKGTTHEGNLADHARIHGLAWQPLVIDSAPAVAEAFFAGRCDAYTTDAA
                     ELAAMRLRAPGGADGFVILPERISKEPLGPVVQGDDPQWTSLVRWVLFALVLAEEHGV
                     TQANVETKVREFGGATGLLLSGHDRRLARALGAQDDWVIRAVRAVGNYGELYERNLGA
                     ASELRIERGLNRLWSDGGLMYAPPID"
     gene            complement(8154..9563)
                     /locus_tag="VIO83_00855"
     CDS             complement(8154..9563)
                     /locus_tag="VIO83_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY8329870.1"
                     /translation="MKGFARRPLVTAMLLSGLLLVGGCMVGPDYQRPPLDVPSAYKEP
                     APTDPAFANLDWWELFADEKLDALINEALANNRDLGVAVSRIEEAAAILRIERANQYP
                     FLDAEAGAGRSSQSRNILPTAETESSYFFNGAASFEVDLWGKLRRSTEAARADLLSSE
                     SSARNVTISLISSVATTYFQLLDLDDRLRISIRTLKSREDALNIIKARYAKGTVPELD
                     VNQAEIEAADAQASVASFDRAVRQTENALSVLVGSRPREIERGTQLAQQAMPLEVPAG
                     LPLNLLERRPDILAAEQQLAAEIARIGVARAQRLPSLSLTASFGYASRDLSDLVEGDS
                     ESWSLFGDLLAPIFNAGQLKSVEQAQRQRAEQARLGYEQAVLNGLRDVDDSLTGLRTS
                     RDEHLARQRQLAAARSAARLSRARYDGGLVSYLEVLDSERSLFQAELLESQTRQQQLS
                     SAVSLYRALGGGWSPSGDQ"
     gene            complement(9560..12727)
                     /locus_tag="VIO83_00860"
     CDS             complement(9560..12727)
                     /locus_tag="VIO83_00860"
                     /inference="COORDINATES: protein motif:HMM:NF000282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEY8329871.1"
                     /translation="MGDFFVRRPIFAMVISIIIVIVGLVAMGSLPIAQYPDITPPEIK
                     VETTYDGANAVNVEQSVATPLEQKVNGVENMLYMKSTNAGNGQMTLSVAFEVGADLDI
                     SNVLVQNRVSEGSAQLPEDVKRRGVKVKKALAFPLMLVTLRSPGESFNADFLTNYMSI
                     NVLDEIARIQGVGQVTIFGGADYAMRIWIKPDQLSRLGLTVPDIVTAVQQQNVLTPAG
                     KLGGPPAATGTEFAYAVQTRGRLETPEQFGQVVVRSNPDGSQVLLRDVARIELGAETY
                     NQIGAFGSKPAAVLAVYQLPDANGLEVAENIRAAMERMKERFPQDIEYVISLDTTKPV
                     SAGIEEIVHTLFEATLIVTVVVFIFLQNFRATLIPTLAVPVALVGTFAVFPLLGFSIN
                     TFSLLGLVLAIGIVVDDAIVVVEGVSEKMERGMPRRQATVETMKEVSGPVIATALSLT
                     AVFVPVAAMSGITGRLYQQFAITIAISVTISAIVALTLSPALSSMLLRPHDHGKKGLL
                     SGFFDRFNRILDKVTDRYIGLTSRFTHRPLRALAVLGVLVVALVLLFRTVPGGFVPEE
                     DQAYLMANLQLPDAASLERTHAAAAKVEAILAADPAIESYTTVTGYSFLSGASSTNNA
                     FFFIQLKDWDERPEAGDQATQVAHRLNGKLAAINEGIAIAFGPPAIPGLGTGSGFSMM
                     LQDRVGNSPDYLAEQTQAFIQAAAKRPEIAGLYTTYRATVPQIYLDVDTQKTMKLGVA
                     PADVNQTLGSFLGGAYVNDFNRFGRLYKVYVQAETDYRKQLADASLFYVRNSAGAMVP
                     MNTLLTDESTNGPEFTYRFNMFRAAEVTGRPAPGYSSGQALQALEEVAAEVLPSDMSY
                     AWNAMSYQEKAAEGSGSMVFVMALVFVFLILAAQYESWSLPLGVLFGTPIAICGAMFG
                     LWLARFFSSSYENNVFAQIGLVMLIGLAAKNAILIVEFARTEIEKGKAPVEAALAAAR
                     LRFRPILMTSFAFILGVIPLILASGAGAEARKIMGMTSFAGMLTATVVGVVLVPGLFV
                     FVERYLGGKKKPKAPAEPVSEEAKP"
     gene            complement(12720..13814)
                     /locus_tag="VIO83_00865"
     CDS             complement(12720..13814)
                     /locus_tag="VIO83_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8329872.1"
                     /translation="MLEVPIVTVAARDVSIPLDLIGETVGSTDVTIRARVDGFLDGIH
                     FKEGTFVDKGALLYTIDPQPLQAKLAQARAMQAQAQTSLVKTKSDLDRIRPLAAMNAV
                     SKLDLDAAVANYEAAKSYVEAARAQVELAQIELGYTRIHAPERGLIGLSEAEVGDFVS
                     QRNNGGLLNVVSRTDPIAVRVSITERGYLNAARRLADQQQGSKLASGTIGQDQRPLSL
                     ILADGSLYEQRGVPTKVDRNIDPTTGALTIEAEFPNAQGLLRPGMFARIRFDADQIKG
                     AILLPQRAVSELQSVHRVFVIKPDDTVEVRQVQVGQRLGSEWVIESGLQAGERVAVAG
                     LLRLRPGMTVKPREANASELPPVAEGGKNG"
     gene            13903..14982
                     /locus_tag="VIO83_00870"
     CDS             13903..14982
                     /locus_tag="VIO83_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329873.1"
                     /translation="MFFATGLPPSTLGAAAAFSHDSHSRPASRVNGASGCAVNVASSS
                     CDSSRLYPCFIKGSPLPAGETAFCGDSFRRHGRDAQAKRGAGGWLLTGSEPPSDGTGQ
                     RKKLHDRNWQHVVFFAYWFWMSLNTGKAGTGMATNERDGNKPTVQRVLDPVSRMSEIL
                     FGLLMVLTFTGTFSVANAGESSVRELLVASLGCNLAWGLVDGCMLLLNRLAERGHNWR
                     MVQHLKTIPLGSAQAFDVLADALPFLVTKVMKAEDVHTLHRRVLEQPIERTSLSLGMS
                     DLLAAVAVFLLVFISTLPVVLPFVLLSEPLHALRASNAIALGMLFLIGMGYGRFAGAR
                     HPGISALVFTVLGIALVAATIALGG"
     gene            complement(15094..15771)
                     /locus_tag="VIO83_00875"
     CDS             complement(15094..15771)
                     /locus_tag="VIO83_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HEY8329874.1"
                     /translation="MKRSNIATGLLLSALLLGGCASKVTQPEQYSGFLPDYSRLQQAT
                     SPTGQPVMRWMAPGVKLDSYDHVMVQSIGFYPVASPSEQVGAPALAELQAYTSQQVKA
                     AFGRRFQVHEPSTTPKGSLPGPKTLILRAAITGVDTKAEGLKPYEIIPIALVAAATTT
                     ATGARDRTTELFVEAELLDASTGQPVLQVVRKGYGKELENTEEQVTLNTLKGVIDGIV
                     RDIEKFE"
     gene            complement(15862..16767)
                     /locus_tag="VIO83_00880"
     CDS             complement(15862..16767)
                     /locus_tag="VIO83_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008367575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8329875.1"
                     /translation="MNMKPHNPISLLLAGLFAVLPMLAGADTLERVRSSNTLTLGYLA
                     NLAPFTSEEGGKAGGYAIEICQKIADRVKSGLGLSDMQVRYQPIGSGKVIEAIGRSEI
                     DLFCSPTPETLERRKVVSFSRPVYTAGMSVMVRKNAPPSLVNVLNGEAVHTGPTWRAT
                     INQGLASHTFVALKGGMSKDWVHDKMRRLGVIATLVTVTSHEEGVKMVAEGKASAFFS
                     ERMLLQNYMQEHAAAGEMMVLDRIFEIAPVSMALARGDEDFRLLVDTVISEMYHSGEL
                     LQLHTKYFGEPGEMLKILSRVYALP"
     gene            complement(16815..18149)
                     /gene="potE"
                     /locus_tag="VIO83_00885"
     CDS             complement(16815..18149)
                     /gene="potE"
                     /locus_tag="VIO83_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015095188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HEY8329876.1"
                     /translation="MAESSKKMSLFGLTTLVAVNMMGSGIIMLPSNMAQLGAVSLLSW
                     IITAIGSMAIAYCFAQCGIYCNKSGGMSAYSEEAHGKSEFFLCSFLYFLSLAIGNVAI
                     GISAVGYLTSFFPWLGSGAIPLFIGTVGLLWITTFANFGGPNITGKIGSITVWGVIIP
                     VAGLSIIGWFWFDPQVFSAAWNPNNIAVSDAITQSIPLTLWAFLGMESAAQSSDAVEN
                     PKRNVPLACLFGTLGAAVVYVLSTTVIQGIIPNADLANSSAPFAYVYAQMFNPVVGNI
                     IMALAVMACVGSLLGWQFTLAQTAKMTADQGLFPRLFSKVTAANAPVIGMIVCGVLQT
                     LLALSTISPNASEQFGKLVSLAAVTNLIPYVTALTGLLVIMHKANVSSAIYSRNVAAL
                     LIAMAYSYYAIYASGKDAVFGATIVLAIGYLLYGFIAKNFVDERPAAAAARA"
     gene            complement(18231..20531)
                     /locus_tag="VIO83_00890"
     CDS             complement(18231..20531)
                     /locus_tag="VIO83_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007996666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8329877.1"
                     /translation="MRHSQTPLPPSLAQRYPVLIVANAHGHEDSIVFHSAQAVMRALD
                     AHELGVELVGSLEDAEIAFSANPAYACVILGWGLCEQDLDLAMKVIRLIRRRTAQLPI
                     MLGMSQAHQSRVPLEFIENIDGFIWLPEDSPEFIAGRIAAAASRYLDTILPPFFGAMV
                     NFADTHEYSWHTPGHTGGTAFMKTAVGRSFLDFYGEQMLRSDLSVSVGELGSLNDHSG
                     PVAAAERYAARVFGADYTFFSVGGSSASNQMVLHSAVTDGDVVLVDRNCHKSLNYALN
                     QSGAVPLYLRPRRNARGLIGPVPQSELTPEAVARKIAESPLARDKQARPVLAVLTNST
                     YDGLCYNVQTTTRELSRTVDRIHYDEAWYAYARFNALYEGRYGMHRGERHADDATVTV
                     THSTHKLLAALSQASMIHIRSGKVPVKPALFNEAFMMHTSTSPQYSIIASIDVSAKMM
                     DDAGEYLTEESIGEAIAFRQAMVRLSNEMRQRDAGDWWFGVWQPDEVDGVPFADLDPQ
                     VLHRGDAWVLKPTAKWHGFGDLGADYCMLDPIKVTVLTPGQTLEGEMQGSGIPAPLVS
                     SFLSSRGIVVEKTEPYSILLLFSVGVTRGKWGSLVAGLMEFKNHYDANTSLEQVMPEL
                     VASHAERYAGMGLRDLAEAMHQEMLASQLLHNMDAAFSLLPDPVASPRDTYARLVRGE
                     IEQIAVRDMQDRTVAVQIVPYPPGIPLMMPGEKAGADKRAIIDYLLAMELFDGRFPGF
                     GHDNHGIEVERDAQGRLTYLVYVVKQ"
     gene            21159..22253
                     /locus_tag="VIO83_00895"
     CDS             21159..22253
                     /locus_tag="VIO83_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8329878.1"
                     /translation="MQPTPTSDQALSRGLLDVLIRAGLVAVLVMFCFQIFRPFLNLML
                     WSLIMAITLYPLHLRVRGKLGNNEGRTATLIVVIAIAILMVPIYLLGASLADSVEQAL
                     TTVRSGNFQIRPPAESVAGWPLVGKPLHAFWLQASTDLTGAAQKFTPQIKELTITLLG
                     KLAGLGMGLLVFIVALLIAGIFMAYGESGRRSAVEIASRLSGPERGPQITELCTATIR
                     AVAQGVVGIAFIQMLLIGVAFVLKGIPGAGLLALAVLLLGIMQLPATLITVPVIAFVI
                     TTEGVSTVNIVFAVYVFIAGLADNVLKPLLLGRGVNVPMPVVLIGALGGMVTNGVIGL
                     FIGPVVLAVGYQLFWQWVRDRPQGGKPGSQ"
     gene            22280..23818
                     /locus_tag="VIO83_00900"
     CDS             22280..23818
                     /locus_tag="VIO83_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY8329879.1"
                     /translation="MSPSLRPGRLLLLGALGLGGCVRLGPDFSPPGEAWVEQWHSPAI
                     EQASQQRAGPDIREWWRVFDDPLLDRLIAEADAHNSSLKIAGLRVMEARAQLGIAESG
                     RYPQLQQATADSVYLDRNQSGGRNPQDSHFWQHSLGFDIGWELDFWGRFSRAIESADA
                     AYFAAEANYEDVLVLLRAQVAETYFALRTTEARLRVARENAAHQRRSYEITERLFTSG
                     ESDELDLQQAKTQYLGTLSSLPEFEDQVARTRNALAVLIGRPPGPLTELAEREGLIPL
                     VDRAVLQDVPADLLRRRPDIRAAELTVAAQSALIGVAKADLYPSLSLLGSIVWSTSSL
                     DGAPDTLDLVGGPSLRWNIFDYGRITNNVRVQDARLQQLIEVYRDSVRQAAREADDAA
                     RGLSKALEREIILREAAISAERSLTLANAQYREGYADFQRVLDAQRALLVQQDNYLVT
                     RNSAVSSLIALYKALGGGWHSAQPRVDPASHAQMQERTNWDDLLDEPAASDAPAPDKE
                     SENE"
     gene            23811..24784
                     /locus_tag="VIO83_00905"
                     /pseudo
     CDS             23811..24784
                     /locus_tag="VIO83_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460100.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         4378 a         8242 c         8044 g         4120 t
ORIGIN      
        1 gtgatcaagg tgctcgagga gtaccgcgtg ccgatcgact gcatcgccgg caccagtatg
       61 ggcgccctgg tcggcgcctc ctacgcgacg ggcacttccg tcgcggaaat ggagacgatc
      121 ctcaagggta tctcgaccga actgctgttc aaggaggctc ccccgcgcca ggaacgcgcc
      181 atgcgccgca aggaggacga ctacggaatt ctgtttaccc ccgaggtcgg actggataac
      241 ggcgaggtga agttcggcaa gggcatcgtg tccggggtgc agctcgaaac cgtactgcgc
      301 cggctcagca aggccaaggg ccactaccag ttcgacaagc tgcccattcc gtatcgcgcc
      361 gtggccaccg acctggtgac cggcaaggcc gtggtgttca gcgagggcga actggccaac
      421 gtgatgcgcg ccagcatgtc ggtacccggg gcggtggcgc cggcggaggt cgacggcatg
      481 atcctggtcg acggcatgct caccagcaac ctgccggtac agaccacccg caacgacatg
      541 ggtgccgaca tcatcatcgc ggtcaacgtc ggtacaccgc tgctgaaacg ggagcagttg
      601 accgggatct tcggcgtatc cgggcagatg ctcagcatct tgaccgagca gaacgtgcag
      661 gcctcactgg cgacgctgac gccctccgac gtgctcatct ccccggatct aggcgatttc
      721 tcgaccgggg atttcgacca tctggagcag atcgccccca tcggcgaagc ggcggcgcgc
      781 aaggtcgccg gccgactcgc ccagctgtcc atcccggctg gcgaatacgc cgcactgcgg
      841 cagcgccagg tgatcaccgt cgtgcccgac ctgcgcccgg tcgacgaaat ccgcgtcgag
      901 aacctcaagc gcgtcaaccc gaagaccgtg caggcgtcca tggccaccaa ggccggcgag
      961 ccgatcgacc agacgacgct cgaccgcgac atgcggcgca tctacggcac cgacgacttc
     1021 gagcacgtga attaccggtt catcgaggaa ccgggtaagc gcatcctggc ggtcgatgcc
     1081 gtggagaaaa cctgggggcc gaactacctg cgcttcggcc tgggcctgtc cagcgacttc
     1141 agcggcgatg cgttcttcaa catcctcggc agctatcgca agacctggct caacgaccgt
     1201 ggcgccgagt ggcgcaccga cgtgcagctc ggccacacca gcggcctgta caccgagctc
     1261 taccagccgt tcagcgccga gagccgcttc ttcgtcgccc cccatgccgc catcgagagg
     1321 cgtacgtccg atctctaccg ggacgacgac cgcgtggcga gctacgagat gacctccatg
     1381 ctcgccggcg tggatctggg cagccagttc aaccgctatg gcgaactgcg cctcggcatg
     1441 gtgggtggac gactcaaacc gcaactggac accggaccgg aaagtctttc gcctggcgag
     1501 tcgcgcatca tgcagggggc attcactggc cagctcaggc tcgaccaact cgatagcgtg
     1561 cacttccccc gctcgggctg gcgcgccggg gcgaacatat tcgattcgac cgacgcgctc
     1621 ggcgcggatg acgactacac caagtgggac ggcgacgcca cagcggtgta ctcgttcggc
     1681 aaccacaccc tcaacttctc aatcaaggtc ggcgacaagc ttggcggcga ccccttgccg
     1741 cgctacgacc agttccagtg gggcggcttc ctgacgggct ccggctacgc caccggccag
     1801 ttgctgggcg aggagctgaa atacggtcgg gtgatgtatt accaccggat tctcgaaggc
     1861 agcatcttcg agggcgccta caccggcttt acactggagg ccactcggat cggcaatccg
     1921 ctggtcccgg gcaacaccga tgactggctc aggtccgctg ccatcttcat cgccgccgac
     1981 agcccgctcg gcccggttta tttgggctac gggcaagcag aggacggcaa cagcagcttt
     2041 tatttctatc tgggcagacc gttttaggtt ctggacgtga atgcaacggc cagctgacga
     2101 ctgctacgtc gcgcagcggt gcaacagcaa tccagcaggg gcagccatgg gcagcgcgaa
     2161 gaactcgacc agcaagacga agaccaaggc cgccgaggcc gcaccgatat tccgctcccg
     2221 ggacgagcgt atagccgctg gtcgggcgtt gcgcgaaaag ctgccgcgca gcgatcacgc
     2281 ggcctggacg ccaccggcca agcaccgcga ccccatcgcc atcctcgaga aatcgaacaa
     2341 ggaccgcctg ccggaactgg tgccgatccg ctacggccgc atgttgcgca gccccttcac
     2401 cttcctgcgc ggctccgcgg cgctgatggc ccacgatctc accaccatgc ccagcaccgg
     2461 catccgggtg caggtctgtg gcgactgcca cctgatgaat ttcggcctgt ttgccacccc
     2521 ggagcgcaac ctggtcttcg atctcaacga ctttgacgaa accctccccg cgccctggga
     2581 atgggacatc aagcggctgg ctgccagctt tgccgtcgcc gggcgtgaca accggctgtc
     2641 caatggcgag tcacgggacg cggctgtggc atgtgtgctc gcctatcggg aaaacctgcg
     2701 cgaatactcc aagaggaacc cgctggaggt ctggtactcg cgcctggacg aagaaaccct
     2761 gatcgagaca gcgcccgatg aaaaggtgag gaagctgcgc cagcagatca ccgacaaggc
     2821 ccgcgaacgg gtggtcgaga atgctttccc caagcttgcc gaacaggttg ccgggagccc
     2881 ccgcattgtc gatcaaccgc cgatcatcta ccacgttgcc gatcccgaat tcatggacaa
     2941 ggtccatgag gggttggcgg aataccgtgc gacgctctct gacgagcgcc gcgtgctcct
     3001 ggaccgctat cgcctggtgg atgctgcgat gaaagtggtg ggcatcggca gcgtcggcac
     3061 ccgctgcttc atcaccctga tgctctccga agaaaaccac ccgctgatcc tgcagttcaa
     3121 ggaagcctgt cactcggtcc tcgagcccta tgcgggggaa agccggtatg acaaccaggg
     3181 ccagcgggtc gtcatggggc aacggctgat gcagtcctcc agcgacattt tcctcggctg
     3241 ggcgcgcggc cggacggggc gtgacttctt cgtacgccaa ttgcgggaca tgaagatgtc
     3301 cgtgcctgtc gagggcgttt cagcggctca actcaaacgc tacgccgggc tctgtggcct
     3361 ggtgctggcc cgggcgcatg ccaagtcggg ggatgccgcg accatcagcg gctatctggg
     3421 caagggcgac accttcgacg aagcgctggg cgacttttcc ctcgcctatg ccgatcagac
     3481 cgagcaggac cacgcgatcc tggtcgaggc tgtgaactcc ggacgagtgg aggcgctcat
     3541 cgaggaggag taacgaccgc cccggcaaac agcgttgacc cgcccctcag cggccggcgt
     3601 cgcgcgcctt caaggcctcg ctgatgaccg tcacatcgag gatgttgcgt acatccactt
     3661 cctcgctgat gacctcctct tcgagcagga aggtcaccag atagccgagg cggcgatgga
     3721 actcggcgcc cttgaccagt gaatagcggt aacgggggtc cacccacagg gtcagatcgg
     3781 gcggcggcga gttctcgtag atcgcctcgg cccacgcctc cttgatgctc atctcccgcg
     3841 ccagcgcctt gatgccgatt tccgggctct tctgcatgac ctcgcgggcc atcagcaacg
     3901 cacgcagaaa gcgcaccgcc gcttcccggt tgttcctgag ccactcgcgc cgggcagcgt
     3961 agaccgatac cgaggtgtag atgcccatcg ccccctcggt gacgatcagc cggccgtccg
     4021 cctcatgcac catcttctgc atccagggtt cccagaccat ggcggcatcg atctgcttgc
     4081 tggccaatgc gctcagttgc tccgcgggcg ccatttcgaa cagttcgacc tggtcgcgac
     4141 ggatgcctag ctggcgcagg gccatcatca gcccgaagtg ggcagtcgag ccccggaaga
     4201 agccgatgcg tttgccggcc aggtcggcga ccgttgctat gccgctgtcc ttgcgggcga
     4261 ccagaccctc gccgtaggca ttctccgggt tgatcccgac caccacccag tcgacgccct
     4321 cagtcagccc gttgaggaag ggcacgctgc cgatggaggc cacgtcgata ctctgctcct
     4381 gcgccgcggc aatcatcggc ggcccggcga cgaacttgac gaagttcggc gtcaagccag
     4441 ccttttcgaa cagctgcaga tccctcgcca ccagcagcaa ccagtcggtg ttcgcctgaa
     4501 aaccgatggt catcggcagc gggctgcgct ccgctgagcg gggattggcg gagaccgctc
     4561 ccagacccgg cagcaacagc aggatgagca gcaccagcag cgtacccttg cgcctcattt
     4621 gcactctcct ttcagcccgt ctcagcgcgt cgtcggaatg gtcaggatgg gcgcggcttc
     4681 atggtctctg gtctccacaa aggtgaagag aaacatgatc gacgagaaca cctgcttgga
     4741 ggggatatcc cggttgtcga tgtagaacca gtagccctgg tacggcacgg ccacgaaggc
     4801 atcgcttggc ggctgggccg aagttgccac ctgaatcagc ggaggaatcg ccccgctggg
     4861 gccgatatcg ccttccgcgg tcggccgcac ccgcttctcc gccacgtgcg catcgggcac
     4921 cgaaatgtac gccgcgatgt cgacgagaat ctcgtagatc gaccgggtca gcagggcgat
     4981 ttcccgatcg ttactggcga cggtcccgta ggtgacctgg tattcccggg ccgcagggtc
     5041 cagccccagc agctgcgcca cattgcgccg cgcgctttcc tcggcggcgg cggtcttcgg
     5101 ccggaagatc atctgcgcct tcgtctcgct gccctgttgc tggagacgca tgccaatgtc
     5161 gcccgacgac tgcaaccgct cgaggttcgg cagcagggca tagaactcgg gattggcccg
     5221 gcgcacgaac aggcgaccaa cccgccggtt gtcgatgccg ttcaccgact gcaccgaggt
     5281 gcggaatacc tcctccaccg ggttgccggc ctggatgatg ttgaggatca ccgagggtgg
     5341 aatcggcgtc atcagcgagc gggcgaagcg ttcgccgctc atgggcgtgt aggtgacggt
     5401 cgggctgtcg ttgaaggtgg ctcccgccgc caggcccacg gaactggtga tggccggggt
     5461 gccgtcagtt tggtttacct ccgcgcttgc accgaaactg cgcgggaacg aataaccggc
     5521 gacgatctgc ccaacatcga ggaacaccgg cgcatcgccg tagcggagct tcaccaggtt
     5581 cagcagcatc tgactcttcc acgagtcgcc caccgcctcc gagtagtcca ggcggtcgcg
     5641 cgccaccgtg cccggcccga tgctcgcgca gccgcagaac aacgtgcaga tcgccagcac
     5701 gccgagtgtc acgccagaga atcctttcat cgtgcaagtg cccctgctag tgagccagcc
     5761 agaacagcgc caccacataa agcgccgata tcgccagctg caccagggtg atcggcacgc
     5821 cgtagcgtga gaagcgcgcg aaggtcacct tctcgccctg cccggcacag atgccgaccg
     5881 tgacaatgtt ggccgaggcg ccgatcaggg tggcgttgcc gcccagcgtg gcgccgaaca
     5941 tcaaggcgat gaataccggc agggtggcag ccggccagtc ggtgaagccc gcaccgaggg
     6001 cgatctccgg caccgcctcg gcggccacca gatagccctt ggtcatcacc agggcggcgg
     6061 ccaccaccgg gatattggcg agcaggctcg acaacaggcc gatgcccgcc agcagggcga
     6121 gcgccaccag ggtgaactcc acgccgaacc actcgtagag cttgagggac agcccctgca
     6181 gcagcccggt ctgggtgaac gcctgcacca ggcagaagat cgcgccgaga aacaccagcg
     6241 tcttccagtc gatatcgcgc aggaccgctt cgaccggctc gacatgcacc gcatagacga
     6301 cgagcagggc caggctggca gcgatgatcg ccaccgaagg cggcacgatg cgcacaggca
     6361 gctgctcgcc gaacaggaac agcaccacca tcagcgccag cacgcccagg gccagcagcg
     6421 cgaatccctt gcgctccagg ggaaccggct gacgcgcggt cggcagctcc acccgcgcct
     6481 tccacacctg cggcatcagc cacggcagca gtggaacgac caccagcaca gccagcaagc
     6541 cagccaggct gaccgtctgc aggtactgga cgaagctcat gccgatcgaa ctgccgacca
     6601 ggaaggtcgc cggatcgccc accaaggtga gcatgccggc ggcgttgctg acgatcgccg
     6661 tgatcaccat cggcccgacg atatccacct tgagcgcccg cgccacacga atgatcaccg
     6721 gcgcgagcag tatcacggtg gtggcgttgg gcaggaacgc gcacaccggg gcgaccagca
     6781 gcaccaacag gatcaggaag cgcttgccac tgccgccggt cgcatgcaga tagatgtcgc
     6841 cgatgcggtc gaacaggccg gtgctggcca gcacccgtgc caccaccatg ccgccgaaca
     6901 gcagggagat cggcccgccg gcgatacgca gggcggcgac cagatcgttg tcggtcagga
     6961 tccccaatgc gagcagcagg cccaccccca gcagcgcggc gaccgccatg tcgagcagat
     7021 cgaaggcgat cgccaggatc accgcagaga agacgccgag ggtcaggtag atctgcagat
     7081 cggtcatcaa ccctctcccc tgtcagtcga tcggtggcgc atacatcagc ccaccgtcgc
     7141 tccacagccg gttaagcccc cgctcgatac gcagctcact agcggcaccg agattgcgct
     7201 catacagctc gccatagttg ccgaccgccc gcacggcccg gatcacccag tcgtcctggg
     7261 cgcccaaggc ccgcgccagc cggcggtcat ggccgctgag caggagcccc gtcgccccgc
     7321 cgaactcccg caccttcgtt tcgacattgg cctgggtcac gccgtgctcc tccgccagca
     7381 ccagggcaaa cagcacccag cgcaccaggc tggtccactg tggatcatcc ccctgcacta
     7441 ccggcccgag cggctccttg gaaatgcgct ccggcaggat cacgaaaccg tccgcaccgc
     7501 ccggcgcgcg caggcgcatg gcggccagtt ccgcagcatc cgtggtgtag gcatcgcaac
     7561 gaccggcaaa gaaggcctcg gccaccgccg gtgccgaatc gatcaccagc ggctgccagg
     7621 cgaggccgtg gatacgggca tggtcggcca ggttgccctc atgggtggtg cccttgccga
     7681 cgcagaccgt cccctccctg aaatcggcca gccgggtgat gccggaggac ttcgccacca
     7741 tgaacgcctg gccgtcatag aacagtaccg ccgggaactg cacctgcagc aacgcctcac
     7801 gggtcagcgt ccaggtggtg ttgcgcacca gcaggtcgat cttgcgcgcc tgcagggcgg
     7861 ggaaacgggt cgaactcctg agcggcacga acttcacctt ctcggcgtcg cccagcagtg
     7921 ccgcggccac cgcgcggcag aagtcggcgt ccagaccgcg ccagcgcccc gagccatcct
     7981 gttcggaaaa gccggggatg ccttcgctca ccccgcagcg caactgatcg cgggctttta
     8041 cgctgtcgag aatttgcccc gcgtgggcgc aagctcccag ccatgcagcg aggaacaatg
     8101 caaagcgccc ccagagtcgc catcgcacgg acacaagacc tccttgccag gactcactgg
     8161 tcgccggacg gcgaccagcc accacccagc gcccggtaca ggctcaccgc cgaactcagc
     8221 tgttgctggc gggtctgcga ctccagcagc tcggcctgga acaggctgcg ctcggaatcc
     8281 agcacctcga ggtagctgac cagtccaccg tcgtagcgtg cgcgtgacag gcgcgcggca
     8341 ctacgcgcag ccgccagctg gcgctggcgg gcgaggtgct cgtcacgcga agtgcgcagg
     8401 ccggtcagcg agtcatccac gtcgcgcaga ccgttcagca ccgcctgctc gtagcccaga
     8461 cgtgcctgct cggcgcgctg acgctgggcc tgctcgaccg acttgagctg gcccgcgttg
     8521 aagatgggcg cgagaaggtc gccgaagagg ctccagcttt cgctgtcgcc ctcgaccagg
     8581 tcgctcagat cacgactcgc gtagccgaag ctggccgtca gggaaaggga tggcaggcgc
     8641 tgcgcgcggg ccacgccgat gcgtgcgatt tcggcggcca gttgctgctc ggcggcgagg
     8701 atatccggcc gacgctccag caggttcagc ggcaggccag ccggaacctc gagcggcatt
     8761 gcctgctgcg ccagctgcgt acctcgctcg atttctcgcg ggcgcgagcc gaccagcacg
     8821 ctgagggcgt tctcggtctg gcgcaccgcg cggtcgaagc tcgccaccga ggcctgggca
     8881 tcggcggcct caatctcggc ttggttgacg tcgagttccg gcaccgtgcc cttggcgtaa
     8941 cgcgccttga tgatgttcag cgcgtcttca cgcgacttca gcgtgcgaat ggagatgcgc
     9001 aggcggtcgt cgaggtccag caactggaag taggtggtgg ccaccgacga aatcagcgaa
     9061 atggtcacat tgcgtgcgct ggattcgctg ctcagcaaat ccgcccgtgc ggcctccgtc
     9121 gagcggcgca gcttgcccca caggtccacc tcgaaggagg cggcgccgtt gaagaagtag
     9181 ctgctctcgg tttcggcggt cggcaggatg ttgcggcttt gcgatgagcg accggcgcca
     9241 gcctcggcgt cgaggaacgg atactggttg gcccgctcga tgcgcaggat cgccgccgcc
     9301 tcttcgatgc gcgacaccgc cacgccgaga tcgcggttgt tggccagcgc ctcgttgatc
     9361 agggcgtcga gcttttcgtc ggcgaacagc tcccaccagt caaggttggc gaacgccgga
     9421 tcggtcggcg ccggctcctt gtaggcactc ggcacatcga gcggcgggcg ctggtagtcg
     9481 gggccgacca tgcagccgcc caccagcagc agcccggaca gcagcatggc cgtcaccagc
     9541 ggccgcctgg cgaatccctt catggcttgg cctcctcgct caccggctcg gcgggtgctt
     9601 tcggtttctt cttgccgccg agatagcgct ccacgaacac gaacagcccc ggcaccagca
     9661 cgacgccgac caccgtagcg gtcagcatgc cggcgaacga ggtcatgccc atgatcttgc
     9721 gcgcctcggc accggcgccg ctggcgagaa tcagcgggat cacaccgagg atgaaggcga
     9781 acgaggtcat caggatcggc cggaagcgca gccgcgccgc cgccagcgcc gcttccaccg
     9841 gcgccttgcc tttctcgatc tcggtgcggg cgaactcgac gatcaggatg gcgttcttcg
     9901 ccgccaggcc gatgagcatc accaggccga tctgcgcgaa cacgttgttc tcgtagctgg
     9961 agctgaagaa gcgcgccagc catagaccga acatcgcgcc gcagatggcg atgggcgtgc
    10021 cgaatagcac gccgagcggc agcgaccagc tctcgtactg ggcagcgagg atcaggaaca
    10081 cgaacaccag cgccatgacg aacaccatgc tgccggaacc ttcggcagcc ttctcctggt
    10141 acgacatggc gttccaggcg taggacatgt cgctcggcag cacctcggcc gccacctcct
    10201 ccagcgcttg caacgcctgc cccgagctgt agcccggcgc cgggcggccg gtgacctcgg
    10261 cggcgcggaa catgttgaag cgataggtga actccgggcc gttggtggac tcgtcggtga
    10321 gcagggtgtt catcggcacc atcgcgccgg cgctgttgcg cacgtagaac agcgaggcgt
    10381 cggcgagctg cttgcggtag tcggtctcgg cctgcacgta gaccttgtac aaccggccga
    10441 agcggttgaa gtcgttgaca taggcaccgc cgaggaagga gcccagggtc tggttgacgt
    10501 cggccggggc gaccccgagc ttcatggtct tctgggtatc cacgtccagg tagatctgcg
    10561 gcaccgtggc ccggtaggtc gtgtacagac cggcgatttc cggacgcttg gctgccgcct
    10621 ggatgaacgc ctgggtctgt tcggccaggt agtcgggact gttgccgacc cggtcctgca
    10681 gcatcatcga gaaaccggaa ccggtgccga ggcccgggat ggccggcggg ccgaaggcga
    10741 tggcgatgcc ctcgttgatc gccgccagtt tgccgttcag ccggtgcgcc acctgggtgg
    10801 cctgatcccc ggcctcgggc cgctcatccc agtccttgag ctggatgaag aagaacgcgt
    10861 tgttggtgga cgaggcgccg gagaggaagc tgtagccggt caccgtggtg taggactcga
    10921 tggccgggtc ggccgccagg atcgcctcga ccttggccgc ggcagcgtgg gtgcgctcca
    10981 gcgaggccgc atccggcagc tgcaggttgg ccatcaggta ggcctgatcc tcctccggca
    11041 cgaagccacc ggggacggtg cgaaacagca gcaccagcgc caccaccagc acgccgagca
    11101 cagccagtgc acgcagcggg cggtgggtga agcggctggt gaggccgatg taccggtcgg
    11161 tcaccttgtc caggatgcgg ttgaagcggt cgaagaagcc cgacagcaac cccttcttgc
    11221 cgtggtcgtg gggccgcaac agcatgctgg acagcgccgg gctcagggtc agcgcgacta
    11281 tcgccgagat agtgaccgaa atggcgatgg taatcgcgaa ctgctggtag agccggccgg
    11341 tgatccccga catggccgcc accggcacga acaccgcggt gagcgacagc gccgtggcga
    11401 tcaccgggcc gctgacttcc ttcatggttt cgacggtcgc ctgacggcgc ggcatacccc
    11461 gctccatctt ctccgacacc ccttccacca ccacgatggc gtcgtccacc acgatgccga
    11521 tggccagcac caggccaagc agcgagaagg tgttgatcga gaagccgagc agcggaaaca
    11581 ctgcgaatgt gccgaccagc gccaccggca ccgccagggt ggggatcagg gtggcgcgga
    11641 agttctgcag gaagatgaac accaccacgg tgacgatcaa ggtggcctcg aacagcgtgt
    11701 ggacgatctc ctcgatgccg gcggacaccg gcttggtggt gtccagcgag atcacgtact
    11761 cgatgtcctg cggaaaacgc tccttcatcc gctccatcgc ggcgcggatg ttctccgcca
    11821 cctccaggcc attggcgtcg ggcaactggt agaccgccag cacggcggcc ggcttggagc
    11881 cgaaggcgcc gatctggttg taggtctcgg cacccagctc tatacgcgcc acgtcgcgca
    11941 gcagcacctg cgagccgtcc ggattggagc gcacgacgac ctggccgaac tgctccggcg
    12001 tttccaggcg gccacgggtc tgcacggcat aggcgaactc ggtgccggtc gcggccggcg
    12061 gaccgccgag cttgcccgcc ggagtcagca cgttctgctg ctgcacggcg gtgacgatgt
    12121 ccggcacggt gagccccagc cggctgagct ggtccggctt gatccagatg cgcatggcgt
    12181 agtcggcgcc accgaagatg gtgacctggc cgacgccctg gatgcgggcg atctcgtcga
    12241 gcacgttgat cgacatgtag ttggtgagga agtcggcgtt gaagctctcg cccggcgagc
    12301 gcaaggtcac cagcatcagc gggaaggcca gcgccttctt gaccttcacc cctcggcgtt
    12361 tgacgtcctc gggcagctgc gcggagccct ccgagacgcg gttctgcacc agcacgttgg
    12421 agatgtcgag atccgcgccg acctcgaagg ccaccgacag ggtcatctgt ccgttgccgg
    12481 cgttggtcga cttcatgtac agcatgttct cgacgccgtt gaccttctgc tccagcggcg
    12541 tggccaccga ttgctcgacg ttcaccgcgt tggcgccgtc gtaggtggtc tccaccttga
    12601 tctcgggcgg agtgatgtcc gggtattggg cgatcggcag gctgcccatg gccaccaggc
    12661 cgacgatgac gatgatgatc gagatcacca tcgcgaagat cggccggcga acgaagaaat
    12721 cacccatttt tgccaccctc agccaccggc ggcagttcgc tggcgttggc ctcgcgtggc
    12781 ttgacggtca tgcccgggcg caggcgcagc aaacccgcca ccgcaacccg ctcgcccgcc
    12841 tgcagtccgg attcgatgac ccattcactg ccgagccgtt gccccacctg cacctggcgc
    12901 acctccaccg tgtcgtccgg cttgatgacg aacacccgat gcacggactg caactcgctg
    12961 accgcccgtt gcggcagcag gatcgcgccc ttgatctggt cggcatcgaa gcggatgcgc
    13021 gcgaacatgc cgggacgcag caggccctgg gcgttgggga actcggcctc gatggtcagc
    13081 gcgccggtgg tggggtcgat gttgcggtcg accttggtgg gcactccgcg ctgctcgtag
    13141 aggctgccgt cagccaggat cagtgacagc ggtcgctggt cctggccgat ggtgccggag
    13201 gccaacttgc tcccttgctg ctggtcggcc agccgccgcg ccgcgttcag gtaaccgcgc
    13261 tcggtgatgg aaacccgcac ggcgatgggg tcggtgcgcg ataccacgtt gagcaacccg
    13321 ccgttgttgc gctggctgac gaagtcgccc acctcggcct cggacaggcc gatcaggccg
    13381 cgctcggggg cgtggatgcg ggtgtagccc agctcgatct gggccagttc gacctgggcc
    13441 ctggccgcct cgacgtagct cttcgccgct tcgtagttgg ccaccgcggc gtccagatcc
    13501 agcttgctca cggcgttcat cgcggccagc ggccggatgc ggtccagatc cgacttggtc
    13561 ttgaccaggc tggtttgcgc ctgggcctgc atggccctgg cctgggcgag cttggcctgg
    13621 agcggctgcg ggtcgatggt gtagagcaac gcacccttgt cgacgaaggt gccttccttg
    13681 aaatggatgc cgtcgagaaa gccgtcgacg cgggcgcgga tggtcacgtc ggttgagccg
    13741 acggtctcgc cgatcagatc caggggaatg ctcacgtcac gcgccgcgac ggtcacaatc
    13801 ggaacttcca gcatggcggc cgctggcggc gcctcgcgcc cgcaaccagc gagcaatagc
    13861 tgcacgagtg cgaacgctat gccgagagac aggcgttgct tgatgttctt tgccacggga
    13921 cttccccctt caacgctcgg tgcggccgcg gctttctcgc acgactcaca cagccggccc
    13981 gcatcccgag tcaatggcgc ttccggatgc gcggtgaatg ttgcttccag ttcttgcgac
    14041 tcaagcaggc tctatccttg ctttataaaa ggtagcccac tgccagcagg cgagacggct
    14101 ttttgcgggg attcttttcg ccgtcacggc cgggacgccc aagccaaacg aggggccggg
    14161 ggatggctac tcacgggcag cgaaccgccg tccgacggca cagggcaacg caagaaactt
    14221 cacgaccgca actggcagca cgttgttttc ttcgcatact ggttctggat gtcactcaac
    14281 acgggcaagg cagggacggg tatggcgacc aacgagcgag acggcaacaa gccaacggtg
    14341 cagcgagtgc tcgatccggt atcgcgcatg tcggaaatcc tgtttggcct gctgatggtg
    14401 ctgaccttca ccggcacctt cagcgtggcc aacgccgggg aaagctcggt gcgcgaactg
    14461 ctggtcgcca gcctgggctg caacctggcc tggggcctgg tagatggctg catgctgctg
    14521 ctgaaccgcc ttgccgagcg tggccataac tggcgcatgg tgcagcacct gaagacaatc
    14581 ccgctcggct ccgcacaggc cttcgacgtg ttggccgatg ccctgccgtt tctggtcacc
    14641 aaggtgatga aagccgaaga cgtgcacaca ctgcaccgcc gtgtgctgga gcagcccata
    14701 gagcggacca gcctgagtct cggcatgagc gacctgctgg cggccgtcgc agtgttcctg
    14761 ctggtgttta tctccaccct cccggtggtg ctgccgttcg tgctgctcag tgagcccctg
    14821 catgccctgc gcgcctccaa tgcgattgcc ctgggcatgc tgtttctcat cggcatgggt
    14881 tacggccgct ttgcgggcgc ccgccacccc gggatcagcg cgctggtgtt caccgtgctg
    14941 ggcattgccc tggtggctgc aaccatcgcc ctcggcggct gagcatccgt cgaaaccagg
    15001 cgaccgagcc aagcacgtgt gggttgagcc cacgagctcc ccccactcaa cacgtcctgc
    15061 ctcggtcccc gcgcactcgc tgtggtgccg gcgctactcg aacttctcga tatcgcgcac
    15121 gatgccgtcg atcacgccct tgagggtatt cagggtgacc tgctcctcgg tgttttccaa
    15181 ttccttgcca tagcccttgc gcaccacctg cagtaccggc tggccggtgc tggcgtcgag
    15241 cagttcggcc tcgacgaaca actcggtggt gcggtcgcgt gcgccggtag cagtggtggt
    15301 ggccgccgca accagggcaa tggggatgat ctcgtagggc ttgagtcctt cggccttggt
    15361 atccacgccg gtaatcgcgg cacgcaggat cagggttttc gggccgggca gagagccctt
    15421 cggcgtggta ctcggctcat gcacctggaa gcgtcggcca aaggcggcct tgacctgctg
    15481 ggaggtgtag gcctgcaact cggccagggc cggagcgccg acctgctcgg acggactcgc
    15541 gacaggatag aagccgatcg actgcaccat cacgtgatcg tagctgtcca acttcacgcc
    15601 gggtgccatc cagcgcatga ccggctgccc ggtgggggaa gtggcctgct gcaggcgcga
    15661 gtagtcggga aggaagccgg aatactgctc gggctgggta actttcgacg cgcagccacc
    15721 gagcagcagt gcgctaagca gtagtccggt ggcaatgttg gaacgcttca tggtgttgct
    15781 cctgtgtctt gacggatcga tcaatagcca agtggtggaa ccgctgagaa catgattgcc
    15841 tcgtccttgt tgtcctctaa cctacggtag cgcatagaca cgggacagga tcttgagcat
    15901 ctcgccgggc tcgccgaagt acttggtgtg caattgcaag agttcgcccg agtggtacat
    15961 ctcgctgatc acggtatcca caagcagacg aaaatcctcg tcgccacgcg cgagggccat
    16021 ggataccggg gcaatttcga agatgcgatc cagcaccatc atctcgccag cggcagcgtg
    16081 ctcctgcatg tagttctgca gcagcatgcg ctcggagaag aacgcgctgg ccttgccctc
    16141 ggccaccatc ttcaccccct cctcgtgact ggtgacggtg accagggtgg cgatgacgcc
    16201 gagtcggcgc atcttgtcgt gcacccagtc cttgctcata ccgcccttga gcgccacgaa
    16261 ggtatggctg gccagcccct ggttgatggt cgcccgccag gtcgggccgg tatgcaccgc
    16321 ttcgccgttg agcacgttga ccagcgacgg cggggcgttc ttgcggacca tcaccgacat
    16381 gccggcggtg tataccggcc gcgagaagct caccaccttg cgccgctcca aggtttccgg
    16441 ggtcgggctg cagaagaggt cgatctcgct gcgaccgatg gcctcgatga ccttgccgct
    16501 gccgatcggc tggtagcgca cctgcatgtc gctgaggcca agaccggact tcacccggtc
    16561 ggcgatcttc tggcagatct cgatggcgta gccgccggcc ttgccgccct cctcgctggt
    16621 gaacggcgcc aggttggcca ggtagccgag ggtcagggtg ttgctgctgc gcacgcgctc
    16681 gagcgtatcg gcaccggcca gcatcggcag cacggcgaac agaccggcga gcagcagact
    16741 gatggggttg tgtggcttca tgttcatatt ctggtcccta cattcagatg tcttggcaat
    16801 ccggatgacc acacctaggc cctggcggcc gcggcggcgg gtcgctcgtc gacaaagttc
    16861 ttggcgatga agccgtagag caggtaaccg atcgccagca cgatggtggc accgaacacc
    16921 gcgtccttgc cggaggcgta gatggcgtaa tacgagtagg ccatcgcgat cagcagtgcc
    16981 gccacgttgc ggctgtagat cgccgagctg acgttggcct tgtgcatgat caccagcagg
    17041 ccggtgagcg cggtcacgta ggggatcagg ttggtcaccg ccgccaggct gaccagcttg
    17101 ccgaactgct cgctggcatt gggcgagatg gtcgacagcg ccagcagggt ctgcagcacg
    17161 ccacagacga tcatgccgat gaccggcgca ttcgctgcgg tgaccttgga gaacagcctg
    17221 gggaacaggc cctggtcggc ggtcatcttg gcggtctgcg ccagagtgaa ctgccagccg
    17281 agcagcgagc cgacgcaggc catcaccgcc agcgccatga tgatgttgcc gaccacgggg
    17341 ttgaacatct gcgcatagac gtaggcgaac ggcgcggagg agttggccaa gtcggcgttg
    17401 gggatgatgc cctggatcac cgtggtcgac agcacgtaca ccaccgcggc gccgagggtg
    17461 ccgaacaggc aggccagcgg cacgttgcgc ttgggattct ccaccgcatc ggagctctgc
    17521 gccgccgact ccatgccgag gaaggcccac agggtcagcg ggatgctctg ggtgatggcg
    17581 tcgctgacgg cgatgttgtt ggggttccag gcggcgctga acacctgcgg atcgaaccag
    17641 aaccagccga tgatgctgag gccggccacc gggatgatca ccccccagac ggtgatcgag
    17701 ccgatcttgc cggtgatgtt cgggccgccg aagttggcaa aggtggtgat ccacagcaag
    17761 ccgacggtgc cgatgaacag cggaatggcg ccactgccca gccaagggaa gaacgaggtc
    17821 aggtagccca ccgcggagat gccgatggcc acgttgccga tcgccagcga caggaagtag
    17881 aggaacgagc agaggaagaa ctccgacttg ccgtgcgcct cctcgctgta ggccgacatg
    17941 ccgcccgact tgttgcagta gatgccgcac tgagcgaaac agtaggcaat ggccatggag
    18001 ccgatcgcgg tgatgatcca cgacagcaac gacaccgccc ccagttgcgc catgttggat
    18061 ggcaacatga tgatgcccga gcccatcatg ttgaccgcca ccaatgtggt gaggccaaag
    18121 aggctcatct tcttgcttga ctcagccata caagactcct tgcgctgtgt gcgacgctgg
    18181 ccctgtaccc ggcgacctgc agcgcgccgc cgggcggggc tgatcagggg ttactgcttg
    18241 accacataga ccaagtaagt gagacgcccc tgggcatcgc gctcgacctc gatgccatgg
    18301 ttgtcatgcc cgaagccggg gaagcggccg tcgaacaact ccatcgccag caggtagtcg
    18361 atgatggcgc gcttgtcggc gccggccttt tcgcctggca tcatcagcgg gatgcccggc
    18421 ggatagggca cgatctgcac ggcgacagtg cggtcctgca tgtcgcgcac ggcgatctgc
    18481 tcgatctcac cgcgcaccag gcgggcgtag gtgtcgcgcg gcgaagccac cggatcgggc
    18541 agcaggctga aggccgcatc catgttgtgc agcagctgcg aagccagcat ctcctggtgc
    18601 atcgcctcgg ccaggtcgcg caggcccatg ccggcgtagc gctcggcatg gctggccacc
    18661 agctcgggca tcacctgctc cagcgacgtg ttggcgtcgt agtggttctt gaattccatc
    18721 aggccggcga ccagcgaacc ccacttgccc cgggtcacgc cgacgctgaa cagcagcagg
    18781 atcgagtacg gctcggtctt ctccaccacg atgccgcggc tggagaggaa ggaggacacc
    18841 agcggcgcgg gaatgccgct gccctgcatc tcgccttcca gggtctgccc cggggtcagc
    18901 accgtgacct tgatcgggtc gagcatgcag tagtcggcgc ccaggtcgcc gaagccatgc
    18961 cacttggcgg tgggcttgag cacccaggcg tcgccgcgat gcagcacctg cgggtcgagg
    19021 tcggcgaacg gcacgccatc gacctcgtcc ggctgccaga cgccgaacca ccagtccccg
    19081 gcgtcgcgct ggcgcatctc gttgctcagg cggaccatgg cctggcggaa ggcgatggcc
    19141 tcgccgatcg attcttcggt gaggtattcg ccggcatcgt ccatcatctt cgccgacaca
    19201 tcgatggagg cgatgatgct gtactgcggc gaggtcgagg tgtgcatcat gaaggcttcg
    19261 ttgaacagcg ccggcttgac cggcaccttg cccgagcgga tgtggatcat cgaggcctgc
    19321 gacagcgcgg cgagcagctt gtgggtcgag tgggtgacgg tcaccgtggc gtcgtcggca
    19381 tgccgctcgc cgcggtgcat gccgtagcgg ccctcgtaca gcgcattgaa gcgtgcgtag
    19441 gcgtaccagg cttcgtcgta gtggatgcgg tcgaccgtcc tgctcagttc gcgggtggtg
    19501 gtctgcacgt tgtagcagag gccgtcgtag gtcgagttgg tcagcaccgc cagcaccggg
    19561 cgggcctgct tgtcgcgcgc cagcgggctc tcggcgatct tcctggccac cgcctcgggg
    19621 gtcagctcgc tctgcggcac cgggccgatc aggccgcgcg cgttgcgccg cgggcgcaaa
    19681 tacagcggca ccgcgccgga ctggttcagc gcgtagttca gcgacttgtg gcagttgcgg
    19741 tcgaccagca cgacgtctcc gtcggtcacc gccgaatgca ggaccatctg gttgctggcc
    19801 gagctgccgc cgaccgagaa gaaggtgtag tcggcgccga acacccgcgc cgcgtagcgt
    19861 tccgccgcgg ccaccgggcc ggagtggtcg ttcagcgagc ccagctcgcc gaccgacacg
    19921 ctgaggtcgg agcgcagcat ctgctcgccg tagaaatcga ggaagctgcg ccccaccgcg
    19981 gtcttcatga aggcggtgcc gccggtgtgg ccgggggtgt gccaggaata ttcgtgggtg
    20041 tcggcgaagt tgaccatggc gccgaagaac ggcggcagga tggtatcgag gtaacggctg
    20101 gcagccgccg caatgcgccc ggcgatgaac tcggggctgt cctccggcag ccagatgaag
    20161 ccgtcgatgt tctcgatgaa ctccagcggc acgcggctct ggtgcgcctg actcatgccc
    20221 agcatgatcg gcagctgcgc ggtgcgccgg cggatcaggc ggatgacctt catcgccaga
    20281 tccaaatcct gctcgcacag gccccagccg aggatcacgc acgcgtaggc cggattggcg
    20341 ctgaaggcga tttccgcgtc ctccagcgag ccgaccagtt ccacgcccag ctcatgggca
    20401 tccagggccc gcatgacggc ctgggcgctg tggaacacga tgctgtcttc atggccatgg
    20461 gcattggcca cgatcagcac cgggtaacgc tgcgcgagag acgggggcaa cggcgtttgc
    20521 gagtggcgca ttgagaaact cctgctacct gttggaagac tgaccgcggc tgtcgccaag
    20581 gacatacgcg cggcagggat tgcacgtgtt ttcttcaggc accaggagta gcagtcacca
    20641 ttcttgtgca cggtggcagc aggtattcga ggtagtctcg ctcttgtcca tttccggaaa
    20701 cgcggcgtac gtccagagtt agtggcagtc gaacgactgc cgcaaatatg gcaagcgaca
    20761 tccctatgct ccgcttttgc gccctgaatt gcagagtagt agcgagcggc caatcgacaa
    20821 gcagaaagca gacaataaat cgccaatcat catgaccaac atcaaggtca cgacttttcc
    20881 gtcataaaac taccgatcag gtgcggcagc ttaagaaccg caaggacacg gtcgctttca
    20941 ttgcgccaag caacttcagg tgctgctgcc gccctccgtc cgccggagaa gtaactatgc
    21001 tgcgcttgtg gaaaccctga acaagccact taccgtcact ccgcgcaggc gggaatccag
    21061 atgctcttgt ggatgcccgc ctgcgcggga atgaccactt cttcagcgtt tccttatcgc
    21121 gcacgcagct ttgctgctta ccggccagga gagaatcgat gcagcccacc cccacttccg
    21181 accaggcgct gtcccgcggc ctgctcgatg tcctgattcg tgccggcctg gtcgccgtgc
    21241 tggtgatgtt ctgcttccag atcttccgcc cgttcctcaa cctgatgctc tggtcgctga
    21301 tcatggcgat caccctgtac cccctgcacc tgagggtgag gggcaagctg ggcaacaacg
    21361 agggccgcac cgcaacactg atcgtggtga tcgccatcgc catcctcatg gtgccgatct
    21421 acctgctggg cgcctcgctg gccgactcgg tggagcaggc cctgactacg gtcaggagcg
    21481 gcaatttcca gattcgtccg ccggccgagt cggtagccgg ctggccgctg gtgggcaaac
    21541 cgctgcacgc cttctggctg caagcctcca ccgacctgac cggtgcggcg cagaagttca
    21601 ccccgcagat caaggaactc accatcaccc tgctgggcaa actcgccggc ctcggcatgg
    21661 gcctgctggt gttcatcgtt gccctactca tcgccggcat cttcatggct tacggcgaga
    21721 gcggacgccg cagcgcggtg gagatcgcct cgcgtctctc cggcccggaa agaggcccgc
    21781 aaatcaccga actgtgcacc gccaccatcc gcgcagtggc ccagggtgtg gtcggcatcg
    21841 ccttcatcca gatgctgctg atcggcgtcg ccttcgtgct caagggcatc cccggcgccg
    21901 gcctgctggc tctggcggtg ctgctgctcg gcatcatgca gttgccggcc accctgatca
    21961 ctgtcccggt gatcgccttc gtcatcacca ccgagggggt cagcacggtg aacattgtct
    22021 tcgccgtcta cgtcttcatc gccggcctgg ccgataacgt gctcaagccg ctgttgctcg
    22081 gtcgtggcgt gaatgtgccg atgccggtgg tgctgatcgg cgccttgggc ggcatggtca
    22141 ccaatggcgt cattggcctg ttcatcggcc cggtggtgct ggcggtcggt taccagttgt
    22201 tctggcagtg ggtcagggac cgcccgcaag gtggcaagcc tggcagccag tagcagacct
    22261 gacattgcca ggcctgccca tgtcgccttc acttcgccca ggccgcctgc tcctgctcgg
    22321 cgcactcggc ctgggtggct gcgtgcgcct ggggccggac ttctcgccgc ctggcgaggc
    22381 ctgggtcgag caatggcaca gccccgccat cgagcaggcc agccagcagc gggccggccc
    22441 cgacatccgc gagtggtggc gggtgttcga tgacccgctg ctggaccgcc tgatcgccga
    22501 agccgacgcc cacaactcca gcctgaagat cgccggcctg cgggtgatgg aagcccgtgc
    22561 ccagctgggc atcgccgaga gcgggcgcta cccacagctg cagcaggcta ccgccgacag
    22621 cgtctacctg gaccgtaacc agtccggtgg ccgcaacccg caggacagcc atttctggca
    22681 gcacagcctg ggcttcgaca tcggctggga gctggacttc tggggccgtt tcagccgcgc
    22741 catcgagtcg gccgatgccg cctacttcgc cgccgaggcc aactacgagg acgtgctggt
    22801 gctgctgcgc gcccaggtgg cggaaaccta tttcgccctg cgcaccaccg aggcgcgcct
    22861 gcgcgtcgcc cgcgagaacg ccgcgcacca gcggcgcagc tacgagatca ccgagcgcct
    22921 gttcaccagc ggcgagagcg acgaactcga cctgcaacag gccaagaccc agtacctggg
    22981 caccctgagc agcctccccg agttcgagga tcaggtggcg cgcacccgca atgccctggc
    23041 tgtgctgatc ggccgcccgc ccggcccgct gacggagctg gccgagcggg aaggcctgat
    23101 cccgctggtc gaccgtgccg tgctgcagga cgtgccggcc gacctgctgc gccgccgccc
    23161 ggacatccgc gccgccgaac tcacggtcgc cgcccagtcg gcgctgatcg gcgtggccaa
    23221 ggccgatctc tacccctcgc tgagcctgct gggcagcatc gtctggtcga ccagttcgct
    23281 ggacggcgca cccgataccc tcgatctggt cggtggcccc agcctgcgct ggaatatctt
    23341 cgactacggg cgcatcacca acaacgtgcg ggtgcaggac gcccgcctgc aacaactgat
    23401 cgaggtctac cgcgacagcg tgcgccaggc ggcgcgcgag gccgacgatg ccgcccgcgg
    23461 cctgagcaag gcgctggagc gcgagatcat cctgcgcgag gcggcgatct ccgccgagcg
    23521 ctcgctgacc ctggccaacg cccagtaccg cgaaggctac gccgacttcc agcgcgtact
    23581 ggatgcccag cgcgcgctac tggtgcaaca ggacaactac ctggtgaccc gcaacagtgc
    23641 ggtgagcagc ctgatcgccc tgtacaaggc cctcggcggc ggctggcaca gcgcccagcc
    23701 gcgggtcgat ccggccagcc acgcgcagat gcaagaacgc accaactggg acgatctgct
    23761 ggacgagcca gcggccagtg acgccccggc gcccgataag gaaagcgaaa atgaatgacg
    23821 aaacgcagcc cgctcccccc gctccggcac ccaagaaggc gacgccgacc gccgacccgg
    23881 tgagcaaggg ggtgaagtgg gtggtttcgg tgatcgtcct gagcctgctc tggtacctgc
    23941 tggccgaccg cttcacgccc tacacccagc aggcgcgggt ggaggccttc gtggtgccgg
    24001 tggccgccga ggtggcgggt cgggtgacca aggtgctggt gcgcgacaac caggtggtcg
    24061 aggccggcgc ggtcctcttc gaggtagatc cgcagcagta ccagatcgcc gtcgagcgtg
    24121 cgcgcgccga cctcgagtcg acccgtcggc agatcgccgc cagcaccgcc ggcatcgact
    24181 cggcacaggc cgcgcagcgc gccgccgagg ccaacgagct gaagtcgaag caggaccggg
    24241 atcgcctcga gcggctgtac cgcgaggacc cgggcaccat ctccctgcgc cgcctcgagg
    24301 tgtctcgcgc gacctacgag cagtccatca gcaggtcgcc gcggcccgcg ccgaagtgca
    24361 gcgggcgcgc gaacagcaag gcggcagtga tgaacagaac gccctgctgc gcagcgccgc
    24421 cacctccctg gagaaggccg agctggactt ggccaatacc caggtgcggg caggctcgac
    24481 cggcctgatc accgacctgc gcaccgatgt cggccagttc gccgcggcgg gcaatccggt
    24541 gatgaccctg atcaccatcc aggacgtgtg gatcagtgca gacatgaccg agaacaacct
    24601 gggacgtctc gagcccgata ctccggtagc catagtgctg gatgtcctgc ctggcgaggt
    24661 gttcgaaggt cgggtacgca gcgtcggcta cggcgtcagc gtcgggcaga gcaccgcccc
    24721 cggcagcctg cccagcgtgg agaacagccg cgactggctg cgcccggcgc agcgcttccc
    24781 ggtg
//
LOCUS       DATCUN010000027        27448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_76178, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000027 DATCUN010000000
VERSION     DATCUN010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 27448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27448
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76178"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VIO83_00910"
     CDS             complement(<1..851)
                     /locus_tag="VIO83_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8329880.1"
                     /translation="MDESKKPTILIVDDNPNNLVLVNNLLKHDYRLKVANNGQTALRI
                     AAAQEQPDLILLDIMMPEMDGYEVCSRLKADPRLRDIPVIFLTARTSIEDEQHGLELG
                     AVDYITKPISPPVMLARIRTHLKLKASADFLRNKSAYLEQEVQRRTRETVAVQDATIE
                     MMASRAETRDNETGNHIRRTQSYVRALAERLRQHPRFADFLTTANIELLFKSAPLHDI
                     GKIGIPDSILLKPGRLDQREFDVMKTHARLGGEAIAQAERMTGRPVPFLHIAKEIAYS
                     HHEKWDG"
     gene            complement(854..3880)
                     /locus_tag="VIO83_00915"
     CDS             complement(854..3880)
                     /locus_tag="VIO83_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8329881.1"
                     /translation="MSIDTRSKLITLMIIVLALLTLGATFQHANLLQEREQAAIQLDE
                     AQAAVDLLADSSDLLTNAVRAYAVTGDEHYRQLFQSELDTFRSREKAVDRLQALGAAP
                     SELDLIAQAKHNSDQLLHLERQAFAAMGGGDRQTALTLVYGVDYVQAKDSIVSPIDEA
                     RHLIDVRLSERIALLSKQARLVDAAALGLLIVTVLAVLASLQLFYRRRLIAPLTSMTA
                     KTRRLLAGDDSVRFDESIHGAEIADLAHALEDSRQASLTIQQQASQLHQQARELASEQ
                     ERIQQTVAWFGSIIESAPDGILVVDDKDTIGLANPKVESLFGYSQGELIGRQLTTLVP
                     GDGHDLYAELRTRALKDGEYLPPMRQQGLHKGGGQFPIEVSLSPLPALGSSPATLCIM
                     IRDSSEREQTERELQMARIQAEKAAQAKSDFLANMSHEVRTPMNSIIGMTHLALQTRL
                     DAQQHDCLRQIQGASEQLMALLDDILDFSRIEAGQLMLEHEPFNLQDLLDSVSQRFEE
                     QARTKGLRFICTAAELPPRLVGDAARIAQILANYVDNAIKFTPQGEVEISMRGRRAGN
                     SELTLEVFVRDTGIGLTEEQQGALFDSFYQADASSTRKYGGTGLGLAIAKRLAELMDG
                     TVGVESQYGRGSTFWFSIPLGIASSSLQQWLPNLDLQGCRVLLAEDNEVNQRLVSKLL
                     QHVGIVVEIAENGSVAVERVRQQDYDLVLMDMQMPVLDGEGATREIRQMGPRGQLPIV
                     AISASAMPQDRQRCFAAGMNDFLPKPLKVADLYASLRQWIKTPPAAPRGADLIAQPAA
                     ETAGPALPAIAGLDTGEGLQRVLGDRTLYLDLLRRLVESEENTLRNIRSALEQNELVT
                     AERLAHTLKGLAGTVGATAVMSGAAELEQALRSQASAALTEHALTRLETRLAPLLSDL
                     QRFFVEHDEPPHAAAIDLQQLAQVCQKLAGYLAEDDAEAVTWLNQQSAALRTAFPDAY
                     PQLEHAINHFEFDKALAFLRSLMAHQQVDSSRPRTA"
     gene            4215..4859
                     /gene="uvrY"
                     /locus_tag="VIO83_00920"
     CDS             4215..4859
                     /gene="uvrY"
                     /locus_tag="VIO83_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HEY8329882.1"
                     /translation="MINVLVVDDHDLVRTGIRRMLADVDGVTVVGEADSGEAALLRAR
                     ELKPDLVLMDVKMPGIGGLEATRKIIRSHPDIRVIAVTACDEEPFPTRLLQAGASGYL
                     TKGAGLEEMIKAIRQVFAGQRYISADIAQQLALKPFQPQADATPFDLLSEREMQIALM
                     IAECQKVQSISDKLCLSPKTVNTYRYRIFDKLEITSDVELAVLAIRHGMVEVDS"
     gene            4861..6687
                     /gene="uvrC"
                     /locus_tag="VIO83_00925"
     CDS             4861..6687
                     /gene="uvrC"
                     /locus_tag="VIO83_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY8329883.1"
                     /translation="MSAVFDSSGFLAACSARPGVYRMFDAEARLLYVGKANNLKKRLA
                     SYFRKSGLAPKTAALVARIAQVETTITANETEALLLEQTLIKEWRPPYNILLRDDKSY
                     PYVFLSSADEYPRLGIHRGAKKQKGRYFGPYPSGLAIRESLNLLQKTFQVRQCEDSYF
                     KNRTRPCLQYQIKRCKGPCVGLVDREEYAEYVRRSVLFLEGRSNVLTDELSAAMETAA
                     AALDFERAAELRDQIALLRRVQDQQSMEGGTGDVDVVAAMVSPGGACVHLITVRGGRV
                     LGSKNFFPQVAIEEAAADVLAAFLGQYYLGSSERDLPNELIVNCEHEDFAALVAAIAE
                     VRGRELSISHRVRGTRARWQQLAVTNAEQALAARLANRQHVAARMEALGEALGMDEPP
                     QRLECFDISHSSGEATVASCVVFGPEGPLKSDYRRFNIEGVTAGDDYAAMHQALTRRF
                     RRLKDGEGKMPDILLVDGGKGQLNMAREVLQELAVVDLVLLGVAKGVTRKPGLETLYL
                     NDAEHEFTLPGDSPALHLIQQIRDEAHRFAITGHRARRGKARRTSSLEEVPGVGPKRR
                     RDLLKHFGGLQELARASVEEIAKAPGISKKLAELIYAVLHSE"
     gene            6721..7281
                     /gene="pgsA"
                     /locus_tag="VIO83_00930"
     CDS             6721..7281
                     /gene="pgsA"
                     /locus_tag="VIO83_00930"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY8329884.1"
                     /translation="MNIPNLLTILRVLLIPVFIGLFYLPFDWRYWAASAVFALASATD
                     WLDGYLARRLGQSTPFGAFLDPVADKLIVAVALVLLVEEHGNLWLTLPAMIIIGREIV
                     VSALREWMAELGARAQVAVSNLGKWKTTAQMVALVILLGNPPRMDFWVGLGYGLLIVA
                     AGLTLWSMVQYLRAAWPHLSNGANGK"
     gene            7346..7421
                     /locus_tag="VIO83_00935"
     tRNA            7346..7421
                     /locus_tag="VIO83_00935"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7379..7381,aa:Gly,seq:gcc)
     gene            7463..7536
                     /locus_tag="VIO83_00940"
     tRNA            7463..7536
                     /locus_tag="VIO83_00940"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7495..7497,aa:Cys,seq:gca)
     gene            7786..8265
                     /locus_tag="VIO83_00945"
     CDS             7786..8265
                     /locus_tag="VIO83_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8329885.1"
                     /translation="MKVLFLVRHAQSSWDDAMLSDRDRPLTDKGKRDAVKMGKRLAER
                     DVVVDMILSSPAKRALATAGAIARKLDFKRKNIVQDERLYAGAVDELLQLIQALDDKQ
                     NRVMLAGHNPTLSELVQRLSGKSTNMPTCALAELRFDMKSWSKIGKSKLEKLVLKYS"
     gene            8508..8939
                     /gene="umuD"
                     /locus_tag="VIO83_00950"
     CDS             8508..8939
                     /gene="umuD"
                     /locus_tag="VIO83_00950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HEY8329886.1"
                     /translation="MPFLNAIRLVPGGQPLPLYGFRIPAGFPSPAQDHLEREISLDEI
                     LDIHAPHTYLVRAAGDSMEGVGIFDGDLMVVDRSREAEPNDIVIAALNSEPLVKRLTC
                     EGRQVVLRAENPRYAPRYILEGDELTIWGVVRFSVRCHEPR"
     gene            8926..10203
                     /gene="umuC"
                     /locus_tag="VIO83_00955"
     CDS             8926..10203
                     /gene="umuC"
                     /locus_tag="VIO83_00955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017002260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HEY8329887.1"
                     /translation="MSRVEPAFALIDCNSFYASCERVFRPDLARTPIVVLSNNDGCVI
                     ARSAEAKPLVKMGEPFFQIRATLRRHGIVAFSSNYALYGDMSQRVMTLLEGMAPALEV
                     YSIDEAFADLSGMPGSLETLGRQMRTTVLRCTGIPVGVGIASTKTLAKLANHAAKRWQ
                     RQTGGVVDLRDPQRRDKLLRAVEVGEVWGIGRKLTEQLAERGIRTAWDLAQADPWTIR
                     KRFSVVVEKTARELRGTACLGLEDEPAAKQEICCSRMFGQRLHELAPIREAVATYAAR
                     ASEKLRAQGSLCKKVRVSIRTGMFNPAEPKFAKGVICELPYPTDDTRLIIRTATEGLE
                     LVYREGFAFSKAEVLLLDLRRCGEYTPDLFTAEQSAAAERVMEVMDKINAKWGRGTMR
                     PASAPAAPDWGMRRELMSQSFTTRVSELWRVGG"
     gene            10434..10521
                     /locus_tag="VIO83_00960"
     tRNA            10434..10521
                     /locus_tag="VIO83_00960"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10467..10469,aa:Leu,seq:taa)
     gene            11064..11324
                     /locus_tag="VIO83_00965"
     CDS             11064..11324
                     /locus_tag="VIO83_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329888.1"
                     /translation="MDDKMSGNHDSPQGECERWDASRAIELELEARDQERIAITKLVE
                     HAHQLIQEVISGKQSHEALAEVSGQLDKAIQILKWPLPRVPS"
     gene            complement(11495..13114)
                     /locus_tag="VIO83_00970"
     CDS             complement(11495..13114)
                     /locus_tag="VIO83_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015093829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8329889.1"
                     /translation="MLRITELKLPLDHTDDDLRAAVLQRLGIADAELLDIQLFKRSYD
                     ARKKSSELLFIYALDVSVRDEAALLAKFADDRQISPTPDMSYKFVGHAPEQLEERPLV
                     VGFGPCGIFAGLVLAQMGFRPIILERGKEVRQRTKDTWGLWRKGVLNPESNVQFGEGG
                     AGTFSDGKLYSQIKDPNFFGRKVLEEFVKAGAPEEILYVNKPHIGTFRLTGMVENMRA
                     EIIALGGEVRFEQKVSDLLIEDGQLRGVQLESGEQIASRHVVLALGHSARDTMRMLHG
                     HGVFMEAKPFSVGFRIEHPQSVIDKARLGKYAGHPKLGAADYKLVHHAKNGRTVYSFC
                     MCPGGTVVAATSEPNRVVTNGMSQYSRNERNANSGLVVGITPEQDYPDGPLAGIELQE
                     RLESLAYELGGRNYEAPAQLVGDFIAGKPSTTLGSVEPSYKPGVKLQDLAAALPPFAI
                     EAIREALPAFDKQIRGYSMHDAVLTGLETRTSSPVRITRGADLQSLNVKGLFPAGEGA
                     GYAGGILSAGVDGIRIAEAVARDLLGLPHDA"
     gene            13860..14699
                     /gene="galU"
                     /locus_tag="VIO83_00975"
     CDS             13860..14699
                     /gene="galU"
                     /locus_tag="VIO83_00975"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY8329890.1"
                     /translation="MIKKCLFPAAGYGTRFLPATKAMPKEMLPVVNKPLIQYAVEEAL
                     DAGLNEISIVTGRGKRAIEDHFDISYELEHQIRDTDKEKYLVGIRRLLSECTFSYTRQ
                     IEMKGLGHAILTGRPLIGDEPFAVVLADDLCLNLEGDGVLAQMVKLYKQFRCSIVAIQ
                     EVPREETHKYGVIAGEMIREDIYRVNAMVEKPKPEDAPSNLAIIGRYILTPDIFDLIE
                     QTAPGKGGEIQITDALMKQAQEGCVIAYKFKGKRFDCGGAEGYVEATNFCFENIYKAG
                     KAY"
     gene            14805..16163
                     /gene="gorA"
                     /locus_tag="VIO83_00980"
     CDS             14805..16163
                     /gene="gorA"
                     /locus_tag="VIO83_00980"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019751004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEY8329891.1"
                     /translation="MAFDFDLFVIGAGSGGVRAARFAAGFGARVAVAESRYLGGTCVN
                     VGCVPKKLLVYGAHVADELEQAGGFGWSVEGARFDWATLIANKNHEIQRLNGIYRNLL
                     VNSGVTLLEGHARILGAHEVEVNGQHYSAEHILVAVGGWPQVPEIPGRELAITSNEAF
                     YLEQLPKRVLVVGGGYIAVEFASIFHGLGSQTTLLYRGERFLRGFDEAVRTHLEEELR
                     KKGMDLQFSSDIAAIERQADGSLRATLRDGRVLEADCVFYATGRRPMLDNLGLESAGV
                     ALDERGFIQVDDEYRSNVPSILAIGDVTGRVQLTPVALAEGMAVARRLYRPAEYRPVD
                     YALIPTAVFSLPNIATVGLTEQQALEQGHRVQVFESRFRAMKLTLTDCQERTLMKLVV
                     DADTDRVLGCHMVGPEAGEIIQGLAVALKAGATKQVFDETLGIHPSAAEEFVTMRTPV
                     SR"
     gene            16177..16554
                     /locus_tag="VIO83_00985"
     CDS             16177..16554
                     /locus_tag="VIO83_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010678616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8329892.1"
                     /translation="MIERLDHLVLTVADIERTVDFYRRVLGMRHEVFGEGRHALAFGQ
                     QKFNLHQAGREFEPKARQLMPGAIDLCLITAWSMAQVQAQLAEQGVAVEEGPVRRTGA
                     LGPIESVYFRDPDGNLIEVSRYL"
     gene            complement(16599..17090)
                     /locus_tag="VIO83_00990"
     CDS             complement(16599..17090)
                     /locus_tag="VIO83_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003188155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY8329893.1"
                     /translation="MLIRTLSLAALLACALPSFAQDYTTGDLTISQPWSRELPPNAPA
                     GAAYFTLHNQGAQADRLIGASTSRAQKSELHTHVQQNGLMKMQQIPAVDIPAKGEVVF
                     QPGGNHVMLFGLGKPLTAGEQFPLTLEFEKAGKVEVQVEVKTADGHAGHGMPMQHGEH
                     SGH"
     gene            17471..18514
                     /locus_tag="VIO83_00995"
     CDS             17471..18514
                     /locus_tag="VIO83_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEY8329894.1"
                     /translation="MARGGINKALVQQARRALLARGEHPSIDAVRVELGNTGSKTTIH
                     RYLKELEETDIGSIKPEKSLSEQLTIQVAQLAERMQEEAHAAVAQEREALAQERAEYL
                     QRLHQSEVRIKQLEGEAEGLTTQLQEVRLAYQQDHQQLQQAMIECARLHQANQDLGVR
                     LADRESQILSLEEKHQHARDAFEHYRLASKEQREQEQRRHEAQVQQLQVETRQLQQVL
                     IIKQDELTQLNRDNERFVTEARQMRKDLAAHQTDLERQAKELASLANRLALSESGKEV
                     LQARANDLQAEVARLVDAVAVQTRQAEALQASLTAMAAQFSQMHHPAQAETANVVSDD
                     SSAEKLESPSLQD"
     gene            complement(18500..19402)
                     /locus_tag="VIO83_01000"
     CDS             complement(18500..19402)
                     /locus_tag="VIO83_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016487120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8329895.1"
                     /translation="MNRLDAMNLFVRVAELGSFSAAACQLGVARSVVTRQIAALEEHL
                     GIKLMVRSTRSLSLTSAGSAYLEKCRVILELVEEAEAGVMEERLTPSGQLRISLPLSF
                     GLRRLVPLLLEFSQAYPEISLAMDFSDRQQNLIEEGIDLSIRITGQLDPGEIVRKLGS
                     SRLLIVAAPDYLARHGRPQQPSGLSGHACLGYSPQANNRPWTFQIDGHLESFYLPYRL
                     QANNGDALAAAAAQGLGITVLPDFIAADYLAAGQLETLLEAFEPPELGIYAVLPSNRY
                     MPHRVRVLIEFLSSRLAAAQSVLE"
     gene            19522..20070
                     /locus_tag="VIO83_01005"
     CDS             19522..20070
                     /locus_tag="VIO83_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016487119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY8329896.1"
                     /translation="MSRQYTGTAKTLHWVIAILILGLLGLGFYMQGLPLSPSKLKLYS
                     WHKWAGVTTFLLVVLRLAWRAGNPPPPLPAHMPKLHQLAAHAGHFALYVLMLAIPLSG
                     WLMSSAKGFQTVWFGVLPIPDLLGRDKALGDLLLTTHLGLNLALIAVLLGHVGAALKH
                     HFVDRDDILTRMLPQTANGTEK"
     gene            20073..20639
                     /locus_tag="VIO83_01010"
     CDS             20073..20639
                     /locus_tag="VIO83_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016487118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY8329897.1"
                     /translation="MKRLALALALISSGLAPSVFAAEYTQVLADKSDIAFSYQQMGVS
                     MDGRFRTFTASQLSFDPAKPEASRAVIDVDLLSIDTGTPENDGEVAGKDWLNSQAFPT
                     ARFEVVSVKALGDNRYEAAGKLTIKGTTRDVVAPATFSEQGDSGVLEGSFTLRRGDYA
                     IGEGEWSAFDIVANDIQVSFRVAVAAGQ"
     gene            20687..21274
                     /locus_tag="VIO83_01015"
     CDS             20687..21274
                     /locus_tag="VIO83_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016487117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY8329898.1"
                     /translation="MNALTKLSAALVLAGAASVSATSAMAAPETYSVDGTHTYPRFSY
                     SHFGMSTQLSRFNKTTGTVVIDKAAKTAKVDITIDMTSVDTGFPTFNEHIQGADFLDT
                     AQFPTATFKSSKVRFEGDKPVALDGDLTIKGVTKPVTLAVSNFVSMPHPMLKKDAIGA
                     DAQVVIKRSEFNAGKYAPHVGDDVTITVALEAIKN"
     gene            21346..22065
                     /locus_tag="VIO83_01020"
     CDS             21346..22065
                     /locus_tag="VIO83_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY8329899.1"
                     /translation="MLEIRPAAARGLANFGWLHSQHSFSFGSYYDPQHMGFSDLLVIN
                     DDRVREGRGFDTHPHRDMEIFSYVLEGALEHKDSMGTGSVIRPGDVQMMSAGTGIRHS
                     EYNASREELVHFLQIWIVPNRKGVTPRYQQQHFDAAEKRGKLRLIISPDGAEGSLSVY
                     QDARVYAGLFDGAEQQRFELPANRFAYIQVARGALTVNGQRLSAGDGARLRNVREIDF
                     SAGDNAEVLLFDLRPNELPLY"
     gene            22098..22697
                     /locus_tag="VIO83_01025"
     CDS             22098..22697
                     /locus_tag="VIO83_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY8329900.1"
                     /translation="MKILQINASARSAGANSTRLADTITARLQAQNPAVVVELRDLAS
                     NPHPVLDEAALGALFTPAEQRTAEQAARVALDDALIAQVQSVDVIVLGVPMYNFGVPV
                     QLKTWLDAIARAGVTFRYTENGPEGLLKGKKVYVALARGGMYRDTPADSQVPYLKTIL
                     GFLGLTDVEFVYAEGLAMGAEGADKAFAAAEAQIGALIA"
     gene            22897..23451
                     /locus_tag="VIO83_01030"
     CDS             22897..23451
                     /locus_tag="VIO83_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020291260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY8329901.1"
                     /translation="MASTDSSSYTATAKWLHWGMAAIWIISWGLGILATHWRDELNPE
                     HGLTILHKALASTLLFLVVVRVAWRLIHPAPALPASMSPLMQRGAVIGHVLLYVIALV
                     ALPFSGWYWSSVADKPILLLGLVQLPPLVAPAPDQYDLAKMIHTWLGWFCGALVGGHL
                     LIALKHHFIDRDAVLTGMLPTCKR"
     gene            23624..24037
                     /locus_tag="VIO83_01035"
     CDS             23624..24037
                     /locus_tag="VIO83_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003250931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY8329902.1"
                     /translation="MRYSLVESQKNEIILLARVLMMILFIMAGWGKLTGFSGTVGYLE
                     SLGTPVPMLAAAIAVIMEFFGAILIVVGFYTRPLALLFALFTVGTAVIGHSFWTMVDA
                     ERAANMTQFLKNMSIVGGLLLLAITGPGKYSLDGK"
     gene            24265..25245
                     /locus_tag="VIO83_01040"
     CDS             24265..25245
                     /locus_tag="VIO83_01040"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY8329903.1"
                     /translation="MGLLVDGQWVDQWYDTESTGGKFVRSTAQFRNWVTADGRPGPSG
                     EGGFAAQPGRYHLYVSLACPWAHRTLIFRVLKGLEELISVSVVNPYMAEHGWTFTPGP
                     GVVADPVGQARYLHEIYLRACADYSGRVTVPVLWDLQRNTIVSNESSEIIRMLNSAFD
                     GVGAAPGDYAPVDLLPQIDEINAHIYDTINNGVYKVGFATDQQVYEREVVALFAALDA
                     LEARLSAQRYLLGERITEADWRLFTTLVRFDAVYHGHFKCNLRRLSGYPNLWAYIREL
                     YQWPGVAATVNLQHIKEHYYRSHGTINPNGIVPLGPVLDLARPHGRDLLG"
     gene            complement(25280..26143)
                     /locus_tag="VIO83_01045"
     CDS             complement(25280..26143)
                     /locus_tag="VIO83_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8329904.1"
                     /translation="MDIELARTFIEIVRCGSFVAAAERLHVTQTAITARIQSLEAQLS
                     SKLFVRNRAGAKLTPDGSAFIAYANQLVQTWESAQRDLPLPEGYGHRLHVGGEVSLSH
                     PLLLQWVGRLREQFPQHAIRAEIGAAAQLLEQLDQGRLDAALVHSPAYWPGLQVEPLL
                     EERLILVRAVNNPEPYVYIDWGEDFRRRHDAALPEHARATLSFNLGPLALQYLLEHGG
                     SGYFRTRVVQAFLERGLLERVTKAPEFSYPIYLVYNRERDSRELQQACELLREVSRQE
                     KDWSQRWDPLI"
     gene            26382..>27448
                     /locus_tag="VIO83_01050"
     CDS             26382..>27448
                     /locus_tag="VIO83_01050"
                     /inference="COORDINATES: protein motif:HMM:NF016843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HEY8329905.1"
                     /translation="MNQWRDNSSRLPAWLPTALHTRPLECLRAAVGVLLGVSASVWLC
                     AWLFDPAVAQMLLGPLGASAVLLFAVSSGALSQPWSILGSYPLAALSGAVAVQLLGAS
                     VASAVLAMAACLLTMFLLRCLHPPAAALSLCVVLAPGLSAQGFAVVLPVLLIAFSLLA
                     CALLFNNLTGVRYPRLSPPVGPSHLTTDPPAGQRVGIASEDLDHALDEFGAFIDVTRE
                     DLATLVRRSEQHALRRQMGELTAARIMSRDLRWVTPETTLDQALQILQYHHLKVLPVL
                     ERDVLVGVVTLADLIELIRSRAEGGLLGYLGRREVRVSRAMTQPAVSVAADTHVVELI
                     PLLSREGLHGLPVLEGGKLVG"
BASE COUNT         4809 a         8461 c         9076 g         5102 t
ORIGIN      
        1 ctgccgtccc atttctcgtg gtggctgtag gcgatttcct tggcaatgtg caggaagggc
       61 accggccggc cggtcatgcg ctcggcctgg gcgatggcct cgccgccgag acgggcatgg
      121 gtcttcatca cgtcgaactc gcgctggtcg aggcggccgg gcttgagcag gatgctgtcg
      181 ggtatgccga tcttgccgat gtcgtgcagc ggcgccgact tgaacagcaa ctcgatgttg
      241 gccgtggtca ggaagtcggc gaagcgcggg tgctggcgca ggcgttcggc caaggcgcgc
      301 acgtagctct gcgtgcggcg aatatggttg ccggtctcgt tgtcgcgggt ttctgcccgc
      361 gaggccatca tctcgatggt tgcatcctgc accgccactg tttcgcgcgt gcgccgctgc
      421 acttcctgct cgagatatgc gcttttgttg cgcaggaagt cggccgaagc cttgagcttc
      481 aggtgggtgc ggatccgggc gagcatgacc ggcgggctga tcggcttggt gatatagtcc
      541 accgcgccca gttccaggcc gtgctgctcg tcctcgatgc tggtacgtgc ggtgaggaag
      601 atcaccggaa tgtcgcgcag ccggggatcg gccttgaggc ggctgcagac ttcgtagcca
      661 tccatttccg gcatcatgat gtccagcagg atcaggtcgg gctgctcctg tgcagcggcg
      721 atgcgcagcg cggtctgccc gttgttggcg accttgagcc ggtagtcgtg cttgagcagg
      781 ttgttgacca gcaccaggtt gttggggttg tcatcgacga tcaggatcgt cggtttcttg
      841 ctctcgtcca tcgtcaggct gttctcggtc tgcttgagtc gacttgctgg tgggccatca
      901 gcgagcgtaa gaatgccagc gccttgtcga attcgaaatg attgatcgca tgctcgagct
      961 gtgggtaagc gtcgggaaag gccgtgcgca gcgccgcgga ttgctggttg agccaggtga
     1021 ccgcctcggc gtcgtcctcc gcgagataac cggccagctt ctggcagacc tgtgccagct
     1081 gttgcaggtc gatggccgcg gcgtgtggcg gctcgtcgtg ctcgacgaaa aatcgctgca
     1141 ggtcgctcag cagaggcgcc aggcgggttt ccaggcgcgt cagggcgtgc tcggtcagcg
     1201 cggccgacgc ctggctgcgc agcgcctgtt ccagctcggc cgcgccgctc atgacggccg
     1261 tggcaccgac ggtgccggcc aggcccttga gggtatgcgc caggcgttcg gcggtgacca
     1321 gttcattctg ttccagtgcg ctacggatat tgcgcagcgt gttctcctcg ctttccacca
     1381 ggcggcgcag caggtccagg tacagcgtgc ggtcaccgag gacccgttgc aatccttcgc
     1441 cggtgtcgag tccggcaatc gccggcaggg cgggccccgc tgtttcggca gcaggctgcg
     1501 cgatcaagtc ggcaccgcga ggggcggctg gcggagtctt gatccattgg cgcagggagg
     1561 catacagatc tgcgaccttg agcggcttgg ggaggaagtc attcatgccg gcggcgaagc
     1621 agcgctggcg gtcctgcggc atggcgctgg cgctgatggc tacgatcggc aactggccac
     1681 gcgggcccat ctggcggatt tcccgggtgg cgccttcgcc gtcgagcacg ggcatctgca
     1741 tgtccatcag caccaggtcg tagtcctgct ggcgcacccg ctcgactgcc acgctgccgt
     1801 tctcggcgat ctcgaccacg atgccgacat gctgcaacag cttgctgacc aatcgctggt
     1861 tgacctcgtt gtcctcggcc agcagcaccc gacaaccctg cagatcgagg ttcggcagcc
     1921 attgctgcag gctactgcta gcgatgccga gcggaatgct gaaccagaag gtgctgccgc
     1981 ggccgtactg gctttcgacg ccgaccgtgc cgtccatgag ttcggcgagc cgtttggcga
     2041 tcgccagccc gaggccggtg ccgccgtact tgcgggtgct ggaagcatcg gcctggtaga
     2101 agctgtcgaa cagtgcgcct tgctgctcct cggtcaggcc gatgccggta tcgcggacga
     2161 agacctcgag ggtgagctcg ctgttgcccg cccgtcgccc tcgcatgctg atctcgactt
     2221 cgccctgggg cgtgaacttg atcgcgttgt cgacgtagtt ggcgaggatc tgggcaatcc
     2281 ttgcggcgtc accgaccagc cgcggcggca gctccgccgc cgtgcagatg aagcgcaacc
     2341 ccttggtgcg cgcctgttcc tcgaagcgct ggctgaccga gtcgaggagg tcctgcaggt
     2401 tgaagggctc gtgctcgagc atcagctggc cggcctcgat ccgcgaaaag tcgaggatgt
     2461 cgtcgagcaa cgccatcagc tgctcgctgg cgccctggat ttgtcgcagg caatcgtgtt
     2521 gctgggcgtc caggcgggtc tgcagtgcca ggtgggtcat gccgatgatc gagttcatcg
     2581 gggtgcggac ttcatggctc atgttggcga gaaagtcgga cttggcctgg gccgctttct
     2641 cggcctggat gcgggccatc tgcagttcgc gctcggtctg ctctcgctcg gagctgtcgc
     2701 ggatcatgat gcacagcgtg gcggggctgc tacccagtgc cggcagaggg gaaagactca
     2761 cctcgatcgg gaattgtccg ccccccttgt gcagcccttg ctggcgcatc ggcggcaggt
     2821 attcgccatc ctttagcgcc cgggtgcgca gttcggcgta gaggtcatgg ccatcgcccg
     2881 ggaccagcgt ggtcagttgc cggccgatca gttcgccctg cgagtagccg aacaggcttt
     2941 cgaccttcgg gttggccagg ccgatggtgt ccttgtcgtc gacgaccagg atgccgtcgg
     3001 gagcggactc gatgatgctg ccaaaccagg ccacggtctg ctggatgcgc tcctgctcgg
     3061 aggccagctc gcgggcttgc tggtgcagct ggctcgcctg ttgctggatg gtcaggctgg
     3121 cctggcgcga gtcttcgagg gcatgcgcca gatcggcgat ttctgccccg tgaatgcttt
     3181 cgtcgaagcg cacgctgtcg tcgccggcca gcagacggcg ggtcttggct gtcatgctgg
     3241 taagcggggc gatcagcctg cgccggtaga acagctgcaa gctggccagc actgccagta
     3301 cggtgacgat cagcaggccg agcgccgcag cgtcgaccag tcgcgcctgc ttcgagagca
     3361 gcgcgattcg ctcggatagg cgtacatcga tcaggtgccg ggcctcatcg atcggcgaca
     3421 cgatggaatc cttggcctgc acataatcga cgccatagac cagcgtcagg gcggtctggc
     3481 ggtcgccccc acccatggcg gcaaaggcct gtctttccag gtggagcagt tggtcggagt
     3541 tgtgcttggc ctgcgcgatc aagtccagct cgctgggtgc cgctcccaat gcctgcaggc
     3601 ggtcgacggc cttctcgcgg gagcggaagg tgtccagctc gctctggaac agctgtcgat
     3661 agtgctcgtc gccggtcacc gcgtaggcgc gtaccgcatt ggtcagcaga tcgctgctgt
     3721 cggcgagcaa gtcgacggcg gcctgcgctt cgtcgagctg gatagcggcc tgctcgcgct
     3781 cctgcaggag gttggcgtgc tgaaaggtgg cgcccagggt gaggagggcg agcacgatga
     3841 tcatcagggt gatcagcttg ctgcgggtgt cgatgctcat ggcggaactc cgcgggggct
     3901 tctcgacgcc agcagctggg ggcgccgcgg atgtcgcgcg tcttcaaaag gttatgattc
     3961 gtcgatttta tgtgacgaga ttcgcataaa acaacaatta atcgctggtt tttaccgccc
     4021 tgcggcccgg ctgctccaga gttgtttaca gcatcggcca tgcgagcgtc gaacaactcg
     4081 cggcgcggcc tgcacagcgc cgacgatcct ccagggcggg cagggcgatc tgtcatttgc
     4141 cggttccatt tgttaaggtt cccggcgatt tcaagattcc cagacaagcc attgtggcat
     4201 taccgaggta tggcgtgatc aacgtgctcg tcgtggatga ccacgacctg gtgcgcaccg
     4261 gcatccggcg catgctggcc gatgtggacg gtgtgactgt ggtgggcgag gccgactccg
     4321 gcgaggcggc cctgctgcgt gcgcgcgaac tcaagccgga tctggtgctg atggacgtga
     4381 agatgcccgg catcggtggc ctcgaggcga cccgcaagat catccgcagt caccccgaca
     4441 tcagggtgat tgccgtcacc gcctgcgacg aggagccgtt tcccacgcgg ctgctgcagg
     4501 ccggcgcgtc gggctacctg accaaggggg cagggctgga ggagatgatc aaggcgatcc
     4561 gccaggtgtt tgccggtcag cgctacatca gcgccgatat cgcccagcag ctggcgctca
     4621 agccgttcca gccgcaggcc gatgcgacgc ccttcgacct gctctccgaa cgcgagatgc
     4681 agatcgccct gatgatcgcc gagtgccaga aggtgcagag catctccgac aagctgtgcc
     4741 tgtcgccgaa gacggtgaac acctaccgct accgcatttt cgacaagctc gagattacca
     4801 gcgatgtcga actggccgtg ctggcgattc gccacggcat ggtcgaagtc gacagctgag
     4861 atgagcgccg tgttcgacag cagcggcttt cttgccgcct gcagcgctcg cccgggcgtg
     4921 taccggatgt tcgatgccga ggcccggttg ctgtatgtcg gcaaggccaa caacctgaag
     4981 aaacgcctgg ccagctactt ccgcaagagc gggctggcgc cgaagaccgc cgcgctggtg
     5041 gcgcgcatcg cgcaggtcga gaccaccatc accgccaacg agaccgaggc gctgctcctg
     5101 gagcagacgc tgatcaagga gtggcggccg ccgtacaaca tcctgctgcg cgacgacaaa
     5161 tcctatccct acgtgttcct gtccagcgcg gacgaatacc cgcgcctggg tatccaccgt
     5221 ggcgcgaaga agcagaaggg gcgctatttc ggtccttacc ccagtggcct ggcgatccgc
     5281 gagagcctca atctgctgca gaagaccttc caggtgcgcc agtgcgagga cagctatttc
     5341 aagaatcgca cccggccctg cctgcaatac cagatcaagc gctgcaaggg gccttgcgtc
     5401 ggcctggtcg accgcgagga gtacgccgag tacgtgcgtc gttcggtgct gttcctcgag
     5461 gggcgcagca atgtgctgac cgacgaactg agtgccgcca tggagacggc cgccgccgcc
     5521 ctcgacttcg agcgtgccgc cgagctgcgt gaccagatcg cgctgctgcg tcgggtgcag
     5581 gaccagcaga gcatggaagg tggcaccggc gacgtcgatg tggtcgcggc gatggtcagc
     5641 cccggcggcg cctgcgtaca cctgatcacc gtgcgtggcg gacgggtgct gggcagcaag
     5701 aacttctttc cgcaggtggc catcgaggag gctgcggccg acgtgctggc ggccttcctc
     5761 ggccagtact atctgggcag cagcgagcgc gacctgccga acgagctgat cgtcaattgc
     5821 gagcatgagg acttcgccgc gctggttgcg gcgattgccg aggtgcgcgg ccgcgagctg
     5881 agcatcagcc accgcgtgcg cggtacccgc gcacgctggc agcagctggc ggtgaccaat
     5941 gccgagcagg cgctggcggc gcggctggcc aaccgccagc atgtcgctgc gcgcatggag
     6001 gcgctcggtg aagcgttggg tatggacgag ccgccgcagc gcctggaatg cttcgacatc
     6061 agccactcca gcggcgaggc cactgtcgcc tcctgcgtgg tattcggccc ggaggggccg
     6121 ctgaagtccg actaccgccg tttcaacatc gagggtgtga ccgccggcga cgattatgcc
     6181 gccatgcatc aagcgctgac ccggcgcttt cgccggctca aggacggcga aggcaagatg
     6241 cccgacatcc tgctggtgga cggtggcaag ggtcagctga acatggcgcg cgaggtgctg
     6301 caggagctgg cggtggtcga cctggtcctg ctcggcgtgg ccaagggcgt gacccgcaag
     6361 ccgggcctgg aaaccctcta cctgaacgat gccgagcacg aattcaccct gccgggcgac
     6421 tcgccggcgc tgcacctcat ccagcagatc cgcgacgagg cgcatcgctt cgccatcacc
     6481 ggccaccggg ctcggcgtgg caaggcgcgg cgcacctcga gtctggagga ggtgccgggg
     6541 gtcggtccca agcggcggcg cgacctgctc aagcatttcg gcgggctgca ggaactggcg
     6601 cgggccagcg tcgaggaaat cgccaaggcc ccgggtatca gcaaaaagct tgcagagttg
     6661 atttatgctg tcctgcacag tgagtagaat gacgcaactc tttgaatatc aaaggtaccg
     6721 atgaatatcc cgaatctgct gaccatcctg cgcgtgctgc tcatcccggt gttcatcggg
     6781 ttgttctatc tgccgttcga ctggcgctac tgggcggcca gtgccgtctt tgcgctggcc
     6841 agtgccaccg actggctgga tggctatctg gcccggcgcc tggggcagag cacgccgttc
     6901 ggtgccttcc tcgacccggt ggcggacaag ctgatcgtgg cggtggcctt ggtgttgctg
     6961 gtcgaagagc acggcaacct gtggctgacc ctgcccgcaa tgatcatcat cggtcgggag
     7021 atcgtggtgt cggccctgcg tgagtggatg gccgagctcg gtgcgcgtgc ccaggtggca
     7081 gtctccaatc tgggcaaatg gaagaccaca gcgcagatgg tggcgttggt catcctgctc
     7141 ggtaaccctc cgcgcatgga cttctgggtt ggtcttggct acggcctgct catcgtcgcg
     7201 gccggcctca ccctgtggtc catggtgcag tacctgcgtg ccgcctggcc tcacctcagc
     7261 aacggtgcca acgggaaata acttttttga atcaaagagt tgacagcggg tccagttcat
     7321 atagaatggc gcccgtcacc acgaagcggg aatagctcag ttggtagagc acgaccttgc
     7381 caaggtcggg gtcgcgagtt cgagtctcgt ttcccgctcc aattttcgtg atgaatgctt
     7441 gcagttaaag tgttcatttt gaggccgggt ggcagagtgg tcatgcagcg gattgcaaat
     7501 ccgtgtacgc cggttcgatt ccgacctcgg cctccagtac agaagccccg tagatcaagc
     7561 atctacgggg cttttttacg tctgcagttt gggcggtttc cgaaactttc ctctatcgtt
     7621 tccgaagcct tgctccgccg cgcagcggcg ctggggcttc cgtgtcggtc agcccccgct
     7681 cgcgggcgat gttgagtaca tggtgcctcg ccccgctgca atggcgctgt aagctgtcag
     7741 cgctagtctc agtggcaagc catcgagcca aataggatgg gtgccatgaa agtgctgttc
     7801 ctggttcgcc acgcgcagtc gagttgggat gacgccatgt tgtccgacag ggatcgccct
     7861 ttgaccgaca agggcaagag ggatgcagtg aagatgggca agcgcttggc tgagcgcgat
     7921 gtggtagtcg acatgattct ttcgagcccg gcgaaaaggg cgcttgcaac cgcgggagcc
     7981 attgccagga agctcgattt caagcgtaaa aacatcgtgc aggatgaacg gctttacgcg
     8041 ggggccgtgg atgaactgct gcagctcatc caggcgctgg acgacaagca gaaccgcgtg
     8101 atgttggccg gtcataaccc gacgctgtcg gagcttgtgc agcgcctttc aggcaaaagc
     8161 accaacatgc caacgtgtgc gctggcagag ctccggtttg acatgaagtc ctggtcgaaa
     8221 atcggcaaat ccaagctcga gaaactggtc ctcaagtatt cgtgacggcg acgtggtcgt
     8281 ccgatggtgg gcgggattga ggggcgttgc tcggaaggag cggcagcccg gtgaccccat
     8341 gcccatccca acttctcttc ctcctcggtg ccggccgcga tccgaagtcg cgggtctgac
     8401 cgataggtat ccggccggag gctgaccggc gcccgctcgt cgttggctct tgtcagtcac
     8461 ggcctggctc gaatactgta tataaataca gtattagaga tgccgccatg cctttcctca
     8521 acgccatccg tctcgtcccc ggcggccagc cattgccgct gtacggcttc cgcatccccg
     8581 ctggcttccc cagcccggcg caggaccatc tggagcggga gatctccctg gacgagatcc
     8641 tcgacatcca cgccccgcac acttacttgg tgcgcgctgc cggcgacagc atggagggcg
     8701 tcggcatctt cgacggcgac ctgatggtgg tcgatcgctc gcgcgaggcg gagccgaacg
     8761 acatcgtgat cgccgcgctg aacagcgagc cgctggtcaa acgcctcacc tgcgagggcc
     8821 gccaggtggt gctgcgcgcc gagaatccgc gctatgcgcc acgctacatc ctggaaggcg
     8881 acgagctgac catttggggt gtggtgcgct tcagcgtgcg ctgccatgag ccgcgttgag
     8941 ccggccttcg cgctgatcga ctgcaactcg ttctacgcga gctgcgagcg ggtgttccgc
     9001 cccgatctgg cgcgcacgcc catcgtggtg ctgagcaaca acgacggctg cgtgatcgcg
     9061 cgcagcgccg aggccaagcc cttggtgaaa atgggcgagc cgttcttcca gatccgcgcc
     9121 acgctgcgcc gccacggcat cgtggcgttc tcctcgaact atgcgctgta tggcgacatg
     9181 agccagcggg tgatgacgct gctcgagggc atggcgccgg cgctggaggt gtacagcatc
     9241 gacgaggcgt tcgccgacct gtcgggtatg ccgggcagcc tggagactct ggggcggcag
     9301 atgcgcacta cagtgctgcg ctgcacgggc attccggtgg gggtggggat tgccagcacc
     9361 aagacgctgg ccaagctggc caaccacgcg gccaagcgct ggcagcggca gacgggcggg
     9421 gtggtggacc tccgtgaccc gcagcgccgc gacaagctgt tgcgggcggt ggaggtgggc
     9481 gaggtgtggg gcatcggccg taagctgacc gagcagctgg cggagagggg tatccgcacg
     9541 gcgtgggatc tggcccaggc cgatccctgg acgatccgca agcgcttttc ggtggtggtg
     9601 gagaagaccg cccgtgagct gcgcggcacc gcctgcctgg gcctggagga cgagccggcg
     9661 gcgaagcagg agatctgctg cagccggatg ttcggccagc gcctgcatga gctggcgccg
     9721 atccgcgagg cggtggccac ctacgccgcc cgggccagcg agaagctgcg tgcccagggc
     9781 tcgctgtgca agaaggtacg ggtgagcatc cgcaccggca tgttcaaccc ggccgagccg
     9841 aagttcgcca agggcgtgat ctgcgagctg ccatacccga ccgacgatac ccggctgatc
     9901 atccgcaccg cgactgaggg tctggagttg gtctaccggg agggcttcgc cttcagcaag
     9961 gccgaggtgc tgctgctcga cctgcgccgg tgcggcgagt atacgcccga cctgttcacc
    10021 gccgagcagt cggccgcggc cgagcgggtg atggaggtga tggacaagat caacgccaag
    10081 tggggcaggg gtactatgcg ccctgccagc gccccggccg cgcccgactg ggggatgcgg
    10141 cgggaattga tgagccagag ctttacgaca cgggttagcg aattgtggag ggtgggggga
    10201 tagccagggc gtccagggct ttctggtgag tctccctatc aggtgtctcg tgtaggccag
    10261 aaaagagtcc cagaggttgg ctgggtcgaa gttcggtcgg gggatttcat gggtgttttg
    10321 gaaacgattt ccgtgtaagc tactgaaatc gcaggatttc agtactgcgc agttgcatat
    10381 cccgatgggg tggcaaaagg gtatggcggg ctgcaatggc ccgcagttgc agggcctggg
    10441 tggtgaaatg gtatacacag cggacttaaa ctccgccgcc gctaaaacgg catacgggtt
    10501 cgagtcccgt cccaggcacc atataccttc agctttcagc aactacccag tgctcggact
    10561 caatgctatt ccttcaaatc gcatcgtggt cctggtcagg cggatcgcct tctgtgcgac
    10621 ggcagtcagc ctgctaccta tgcaatcgtc gctcggtcca tcgcctaaac aggattaaaa
    10681 gcgtagctgc gggggggggg ctttttggct tcgtccagcg cggggcttgt cgtttccacc
    10741 cgcagtgcca ccttcctgct tggatccgca tccgttctgg ccctgtctcg ccacctcgaa
    10801 atcaaagcgt ccatatgcac tagacttcta tacgtaaaac gtcgcaatga ctcacggcaa
    10861 atcttcttag tagatttcca gatatcgtca agtggtcggc agcaaataca tggcacgcgg
    10921 cactctcggg gccaattaat tgcgggcatg cctcgcgtag gggccggcca ctgctgggct
    10981 gctggcgagc ttgacagagc ctcgagctgg cagccgcggc agcaacactc gctaaataac
    11041 aatgacaaca agagggaacc acgatggacg acaagatgag tggaaaccat gacagcccgc
    11101 agggggagtg cgagcggtgg gatgctagtc gtgcgatcga gctggagttg gaggctcgag
    11161 accaggagcg cattgccatt accaaactgg tggagcatgc gcatcagctg attcaggaag
    11221 taatcagcgg caagcaaagc cacgaggcgc tggcagaggt tagtggccag ttggacaagg
    11281 caattcagat tctcaagtgg ccactcccgc gtgtcccatc ctgaattgac ccgtcggaga
    11341 gcgtctgggc gcttctctcg acagcggtga caagcaagca tgaccggtga actgcgccag
    11401 cggggagcgc agttcaccgt acgtggctat ctgcagtcga gcctccggag gttttcagcc
    11461 tcaggaggaa gcggcttgca gacgtagtcg ccattcaggc gtcgtggggc agccccagca
    11521 ggtcgcgggc cacggcttcg gcgatgcgga tgccgtcgac cccggcggag aggatgccac
    11581 cggcatagcc ggcgccttcg ccggccggga acagaccctt gacgttgagg ctctgcaggt
    11641 cggcgccgcg ggtgatgcgc accggcgagg aggtgcgtgt ctcgaggccg gtgagcaccg
    11701 cgtcgtgcat cgagtagccg cggatctgct tgtcgaaggc cggcagggcc tcgcggatcg
    11761 cctcgatggc gaagggcggc agagccgcgg ccagatcctg cagctttacc cccggcttgt
    11821 acgacggctc gacgctgccc agggtggtgg acggcttgcc ggcgatgaag tcgccgacca
    11881 gctgcgccgg ggcctcgtag ttgcgaccgc cgagctcata ggcgagggac tccaagcgct
    11941 cctgcagctc gatgccggcc agcgggccgt ccgggtagtc ctgctcgggg gtgatgccca
    12001 ccacgagacc ggagttggcg ttgcgctcgt tgcgcgagta ctggctcatg ccgttggtca
    12061 ccacccggtt cggttccgag gtggcggcca ccacggtgcc gccggggcac atgcagaagc
    12121 tgtacaccgt gcggccgttc ttggcgtggt ggaccagctt gtagtcggcg gcgccgagct
    12181 tggggtggcc ggcatacttg cccaggcgcg ccttgtcgat caccgactgc gggtgctcga
    12241 tgcggaagcc cacggagaac ggcttggcct ccatgaacac gccgtggccg tggagcatgc
    12301 gcatggtgtc gcgggcgctg tggccgaggg ccagcaccac atgccggctg gcgatctgct
    12361 cgccgctctc cagctgcacg ccgcgcagct ggccatcctc gatcagcagg tcgctgacct
    12421 tctgctcgaa gcgcacctcg ccgccgaggg cgatgatctc cgcacgcatg ttctcgacca
    12481 tgccggtgag gcggaaggtg ccgatgtgcg gcttgttgac gtagaggatc tcttccggag
    12541 cgccggcctt gacgaactcc tccagcacct tgcgaccgaa gaagttgggg tccttgatct
    12601 ggctgtacag cttgccgtcg gagaaggttc ccgcaccgcc ttcgccgaac tgcacgttgg
    12661 actcgggatt gagcacgccc ttgcgccaca ggccccaggt gtccttggtg cgctgtcgca
    12721 cttccttgcc gcgctcgagg atgatcggac gaaagcccat ctgcgccagc accaggccgg
    12781 cgaagatgcc gcacgggccg aagcccacca ccagcggacg ctcctccagc tgctcgggcg
    12841 cgtggccgac gaacttgtaa ctcatgtccg gggtagggct gatttgccgg tcgtcggcaa
    12901 atttggcgag cagggcagct tcgtcacgca cgctgacgtc cagggcgtag atgaacagca
    12961 gctcgctgga cttcttgcgg gcgtcgtagc tgcgtttgaa cagctggatg tcgagcagct
    13021 cggcatcggc gatgcccagg cgctgcagca cggcggcgcg caggtcgtca tcggtgtggt
    13081 cgaggggcag tttgagttcg gtgatgcgca gcatgggggc gtccacgtca gggccggagg
    13141 caacccggca gcttttgaaa ggcgccgatt atacgcgcga tcggcggctt ttcggcaggg
    13201 tctgggggcg ggccaacagc acgtggagga gcctgccggt gcagcacctg caaacggatc
    13261 catgagccag agccagtggc aggcgatgca acttcagcag ctcatgagcg agaagggctg
    13321 tcctatgagg agtaccagcg tcggcatcga ctcatttatg ggtcgcaatg atcggatcgc
    13381 agcaacttat tgaagagtcc agatcaaggc gactgttccg gcgttggccg agcggatcgc
    13441 actgatccga atacgcctac ttgctcgcgg atatcctggg caagatgggg caccccaaaa
    13501 agccgacttt taaggtcggc ttttttacgc ctgaatttta actggctcgc tggtgcactg
    13561 ggcgagacgt ttggcgctgc gcttgctcag catttctgac tatgaccgga tcgcgatacg
    13621 tgtttctatg atgcgcaatt taacataatg aaatttatat ccttgcatgg atacatctaa
    13681 ccttgtgctc tccccactag tcgacatgta gaagtcgtct tcccgcgcgc atgggaaggg
    13741 tgactattga aaagaagccg cacacggggc gatatgcatc acgggcagtg attgtaaggc
    13801 gcgagcgccc tgattagact gcgcgatcag aactcccaca cctgcaaggc ttctctccca
    13861 tgatcaagaa gtgtctcttc ccggcggctg gttacggtac tcgttttctg ccagcgacca
    13921 aggcgatgcc caaggaaatg ctgccggtgg tgaacaagcc actgatccag tatgccgtcg
    13981 aggaagcgct ggatgccggc ctgaacgaga tctccatcgt gaccggccgc ggcaagcggg
    14041 cgattgaaga ccatttcgat atcagctacg agctggaaca tcagatccgc gacaccgaca
    14101 aggaaaagta cctggtcggt atccgtcgcc tgctcagcga gtgcactttc tcctataccc
    14161 gccagatcga gatgaagggt ctgggccatg cgatcctcac cggccgcccg ctgatcggcg
    14221 acgagccctt cgccgtggta ctggccgacg acctgtgcct gaacctggaa ggtgatggtg
    14281 tactggcgca gatggtcaag ctgtacaagc agttccgctg ctccatcgtc gccatccagg
    14341 aagtgccgcg cgaggaaacc cacaagtacg gcgtgatcgc tggcgagatg attcgcgagg
    14401 acatctaccg ggtcaacgcc atggtcgaga agcccaagcc ggaagacgcg ccgtccaacc
    14461 tggcgatcat cggtcgttac atcctgactc cggacatctt cgacctgatc gagcagaccg
    14521 cgccgggcaa gggtggcgag atccagatca ccgacgcact gatgaagcag gcccaggaag
    14581 gctgcgtgat cgcctacaag ttcaagggca agcgtttcga ctgcggtggc gctgagggct
    14641 atgtcgaggc gaccaacttc tgcttcgaga acatctacaa ggccggcaaa gcctactgat
    14701 tgagccgcag tatcgctgga tagctgaccg ccctgtacct aacggtgcag ggcggttttg
    14761 tttgggcggg tgatatgctt cccgcacatg gcaaggagac cgacatggca ttcgatttcg
    14821 acctgtttgt gattggcgct ggctccggcg gcgtacgtgc ggcgcgtttt gccgccggct
    14881 tcggtgcacg agtggcggtg gcagagagcc gctatctggg cggcacctgc gtgaacgtcg
    14941 gctgtgtgcc gaagaagctg ctggtctacg gcgcccacgt cgccgacgag ctggagcagg
    15001 ccggtggctt cggctggtcg gtcgagggtg cgcgcttcga ctgggcgacc ctgattgcca
    15061 acaagaacca cgaaatccag cgcctcaacg gcatctatcg caatctgctg gtgaacagtg
    15121 gggtgacgct gctggaaggg catgcgcgca tccttggcgc ccacgaggtc gaggtgaacg
    15181 gccagcatta cagcgccgag cacatcctgg tcgcggtcgg tggctggccg caggtgccgg
    15241 agatcccggg tcgcgagctg gcgatcacct ccaacgaagc cttctatctc gagcagctgc
    15301 ccaagcgcgt actggtggtc ggcggcggtt atatcgccgt cgagttcgca tcgatcttcc
    15361 acggcctggg gtcgcagacc acgctgctgt accgcggcga gcgcttcctg cgcggcttcg
    15421 acgaggcggt gcgcacccat ctggaggaag aactgcgcaa gaagggcatg gacctgcagt
    15481 tcagcagcga catcgccgcc atcgagcgcc aggccgacgg cagcttgcgg gcgactctgc
    15541 gcgacggtcg ggtgctggaa gccgattgcg tgttctacgc cactggccgc cggccgatgc
    15601 tggacaatct ggggctggag agtgccggcg tagcgctgga cgagcggggc ttcatccaag
    15661 tggatgacga atatcgcagc aacgtgccct cgatcctcgc catcggcgac gtgaccggcc
    15721 gcgtgcagct gacgccggtg gcgctggccg agggcatggc ggtggcgcgc cggctgtacc
    15781 gtcccgcgga ataccgtccg gtggactacg cgctgattcc tactgccgtg ttcagcctgc
    15841 ccaacatcgc caccgtcggc ctgaccgagc agcaggcgct cgagcagggt caccgggtgc
    15901 aggtgttcga gagtcgcttc cgcgccatga agctgaccct gaccgactgc caggagcgca
    15961 ccctgatgaa gctggtggtg gatgccgaca cggatcgcgt gctcggttgc cacatggtcg
    16021 gcccggaagc cggcgagatc atccaggggc tggcggtggc actgaaggca ggggcgacca
    16081 agcaggtgtt tgacgaaacc ctcggcatcc acccctcggc ggccgaagag ttcgtcacca
    16141 tgcgcacgcc ggtgagccgc tgagcatggg gcagccatga tcgagcgcct ggaccatctg
    16201 gtccttaccg tggcggacat cgagcgcacg gtcgatttct atcggcgcgt gctgggcatg
    16261 cgccatgagg tcttcggcga gggccggcat gccttggcat tcggtcagca gaaattcaac
    16321 ctgcaccaag ccggccgcga gttcgagccc aaggcgcggc agctgatgcc gggcgccatc
    16381 gacctgtgcc tgattacggc atggtcgatg gcgcaggtgc aggcgcagct ggcggagcag
    16441 ggtgtggctg tagaggaagg gccggtaagg cgtaccggcg cgctgggtcc gatcgagtcg
    16501 gtgtactttc gcgatccaga tggcaacctg atcgaggtca gtcgctacct ctgagcatca
    16561 gggtgcccgg cagcggcggc gctgccgggc gctaacggtc agtggccgct gtgttcgccg
    16621 tgctgcatgg gcatgccgtg accggcgtgg ccatcggcag ttttgacttc aacctgcacc
    16681 tcgaccttgc cggccttctc gaactcgagc gtcagcggga actgctcgcc cgcagtcagc
    16741 ggcttgccca aaccgaacag catcacgtga ttgcctcccg gctggaacac cacctcgccc
    16801 ttggcgggaa tgtcaacggc cggaatctgc tgcatcttca tcaaaccgtt ctgctgcaca
    16861 tgggtgtgca gctcgctctt ctgcgcgcgc gaagtgctgg ccccgatcag tcgatcggcc
    16921 tgggcgccct ggttgtgcag ggtgaaatag gcggcgccgg ccggcgcatt cggcggcagc
    16981 tcgcgcgacc acggctggct gatggtcagg tccccagtcg tgtagtcctg ggcgaagctc
    17041 ggcagggcgc aggcgagcag ggcggccagg ctcagggtgc gaatcagcat tgttggtgac
    17101 tcctcgtgat ggcggatggc ccatgggccg ttgcgcgcga ccatagcaca gccggcgcga
    17161 cgactgccgc gaccgatggg cgcagcatga ggcgttccgc gtggccgcgc aggcctgccg
    17221 gtgcttttcc tcccaagagt tgatatgcgc atcgccgttc gggcgttacg gctcgatcac
    17281 cgcaattcat cgccggcacc agccccaaac gtactcgaaa acggagctac gcacgctcca
    17341 ttgcggccta tcacttggta attcgtgatt atcaagtctc tgtagaaatt ggaaaatcgg
    17401 tatttcttgg tttaaaaatg ccatgtaatt acgtattatg taccacagta tcaaatcgta
    17461 ggggcacact atggctcggg gcggcatcaa caaggcgttg gtccagcaag cgcgacgcgc
    17521 gctgctggct cgtggcgagc atccaagcat cgatgcggtg cgtgtcgagc tgggaaatac
    17581 cggctccaag accaccatcc accgctatct gaaggagctg gaagagacgg atattggttc
    17641 gattaagccg gaaaaatcgc tgagtgagca gctaaccatc caggtcgccc agctggcgga
    17701 gcggatgcag gaagaggccc atgctgccgt ggcccaggag cgggaggcgt tggcgcagga
    17761 gcgcgctgag tacctgcagc gcctccatca gtcggaggtc cgcatcaagc agctggaggg
    17821 ggaggctgag ggcttgacca cgcagttgca ggaggtgcgt ctggcgtacc agcaagacca
    17881 tcaacagttg cagcaagcga tgattgagtg cgcccgactg catcaggcga atcaggatct
    17941 gggcgtacgg ctggccgatc gagagtcgca gatcctctcg ctcgaagaga agcaccagca
    18001 tgcccgtgat gcctttgagc actatcgact ggccagcaag gagcagcgcg agcaggagca
    18061 gcggcgtcac gaggcgcagg ttcagcagct ccaggtggaa actcgccagc tgcagcaggt
    18121 cctgatcatc aagcaggacg aattgactca gctgaaccgt gataacgagc gcttcgtgac
    18181 tgaggcaagg cagatgcgaa aggatctggc cgcccatcag accgacctgg aacgccaggc
    18241 gaaggagctc gccagtctcg cgaatcgact cgcactgtcg gagtcaggca aggaggtgct
    18301 gcaggcgcgg gctaacgatc tccaggctga agtggcgcgc ctggtggatg ccgtggcagt
    18361 gcagacgcgg caggcagaag cgctgcaggc cagcctgacc gcgatggctg cccagttcag
    18421 ccaaatgcat catccagcgc aagctgagac cgccaacgtc gtcagcgacg acagcagtgc
    18481 cgagaagctc gagtcgccat cactccaaga ctgattgcgc ggcagccagc cggctcgaca
    18541 gaaactcgat cagtacccgg acacgatgag gcatgtagcg gttgctgggc agcaccgcat
    18601 agatgccgag ctccggcggc tcgaacgctt ccagcagggt ctccagctgg cctgccgcga
    18661 gatagtcggc ggcgatgaag tccggcagca cggtgatgcc gaggccctgt gcggcggcag
    18721 cggccagcgc gtcgccgttg ttggcctgga ggcggtaggg caggtagaag gactccagat
    18781 ggccgtcaat ctggaaggtc caggggcggt tgttcgcctg tggcgagtag ccaaggcagg
    18841 catggccgct gagtccggag ggctgctgtg ggcgtccatg ccgggccagg tagtccggag
    18901 cggcgacgat cagcagccgg ctgctgccga gcttgcggac gatttccccc ggatcgagct
    18961 ggccagtgat gcgaatcgac agatcgatgc cttcctcgat caggttctgc tgccggtcgc
    19021 tgaagtccat cgccaggctg atctcggggt aggcctgcga gaactccaga agcagcggca
    19081 ccagccggcg caagccgaag ctgagcggca gactgatgcg cagttgcccg ctgggggtca
    19141 gccgctcctc catcaccccg gcctcggcct cttcgaccag ctcgagaatc acccggcact
    19201 tttccagata ggcgctgcct gccgaggtta gcgacaggct ccgcgtgctg cgcaccatga
    19261 gcttgatgcc caggtgctct tccagtgccg cgatctgccg ggtaaccacc gagcgtgcca
    19321 cgccgagctg gcaggcggcg gccgagaaac tgcccagttc ggccacccgc acgaacaggt
    19381 tcatcgcatc gagtcggttc attgttgatt ctcacgtcaa cagagtgttc ttgattgtcc
    19441 tctatctggc atcagtgcgc acgcgtaaag tttccccatc gccgatgccc acaacgcatc
    19501 gctgcaccga ggacgatgaa cgtgagccgc caatacaccg gaaccgccaa gaccctgcac
    19561 tgggtgatcg cgatcctgat cctggggctg ctggggctgg gcttctacat gcagggcctg
    19621 ccgctgtcgc cgagcaagct gaagctgtac tcctggcaca agtgggccgg ggtgaccacc
    19681 ttcctgctgg tggtgctgcg cctggcctgg cgggcaggca atccgccgcc gccgctgcca
    19741 gcccacatgc cgaagctgca ccagctggcc gcccatgccg ggcacttcgc gctgtatgtg
    19801 ctgatgctgg ccatcccgct gtcgggctgg ctgatgagct cggccaaggg cttccagacc
    19861 gtgtggttcg gcgtgttgcc gatccccgac ctgctcggtc gcgacaaggc actcggtgac
    19921 ctgctgctga ccacccacct ggggctgaac ctggcgctga tcgccgtact gctcggccac
    19981 gtcggtgccg ccctgaagca ccacttcgtc gaccgcgacg acatcctcac tcgcatgctg
    20041 ccgcagaccg ccaacggcac ggagaaatga acatgaaacg ccttgctctc gctcttgcgc
    20101 tgatctcctc cggcctggcc ccgagcgtct tcgctgccga atacacgcag gtgctggccg
    20161 acaagagcga catcgccttc agctatcagc agatgggcgt gtcgatggac ggccgcttcc
    20221 ggacctttac cgccagccag ctgagcttcg acccggccaa gccggaagcc agcagggcgg
    20281 tcatcgacgt cgatctgctg agcatcgata ccggcacgcc ggaaaacgat ggcgaagtcg
    20341 ccggcaagga ctggctcaac agccaggctt tcccgaccgc ccgtttcgaa gtggtcagcg
    20401 tcaaggcgct cggcgacaac cgttacgagg ccgccggcaa gctgaccatc aagggcacga
    20461 cccgcgatgt cgtcgccccc gccaccttca gcgaacaagg tgatagcggt gtgctggagg
    20521 gcagcttcac cctgcggcgc ggcgactacg ccatcggcga gggcgagtgg tccgctttcg
    20581 acatcgtcgc caacgacatc caggtcagct tccgcgtcgc cgtagcggct ggccagtaat
    20641 ccgctcaacc accccgaatt caacccagac accacaggaa tcccacatga acgctctgac
    20701 caaactgtcc gccgccctgg ttctcgccgg tgccgccagc gtttccgcca cctccgcgat
    20761 ggccgcgccg gaaacctaca gtgtcgatgg cacccacacc tacccgcgct tctcgtacag
    20821 ccacttcggc atgtcgaccc agctgagccg cttcaacaag accaccggca ccgtggtgat
    20881 cgacaaggcc gccaagactg ccaaggtcga catcaccatc gacatgacct cggtcgatac
    20941 cggcttcccg accttcaacg agcacatcca gggcgccgac ttcctcgaca ccgcccagtt
    21001 cccgactgcg accttcaagt cctccaaggt gcgcttcgag ggtgacaagc cggtggccct
    21061 ggacggcgac ctgaccatca agggcgtgac caagccggtc accctggccg tgagcaactt
    21121 cgtcagcatg ccgcacccga tgctgaagaa ggatgccatc ggcgccgacg cccaggtggt
    21181 catcaagcgc agcgagttca acgccggcaa gtacgccccg cacgtgggcg acgacgtgac
    21241 catcaccgtc gccctcgaag cgatcaagaa ctaacaccct gaagctgcgg ggcagggggg
    21301 ccatctgagg ctccccgtcc cacagccggc acgcaggaga acgacatgtt ggaaatcaga
    21361 cctgccgcag cgcgcggtct tgccaatttc ggctggctgc actcgcagca cagcttttcc
    21421 tttggcagct actacgaccc gcagcacatg ggcttttcag acctgctggt gatcaacgac
    21481 gaccgcgtcc gcgagggccg cggcttcgac acccacccgc accgcgacat ggaaatcttc
    21541 tcctacgtgc tcgaaggcgc gctggaacac aaggactcga tgggcaccgg ctcggtgatc
    21601 cgccccggcg acgtgcagat gatgagcgcc ggcaccggca tccggcacag cgagtacaac
    21661 gcctcgcgtg aggagctagt gcacttcctg cagatctgga tcgtgccgaa ccgcaagggc
    21721 gtcacgccgc gctaccagca gcagcacttc gatgctgccg agaagcgtgg aaagctgcgc
    21781 ctgatcattt ccccggacgg cgccgaaggc tcgctgtcgg tctaccagga tgcccgtgtg
    21841 tatgccgggc tgttcgatgg tgccgagcag cagcgcttcg agctgccggc caatcgcttc
    21901 gcctacatcc aggtggcgcg cggcgcgctg accgtcaacg gccagcgcct gagcgccggc
    21961 gacggtgcgc gcctgcgcaa cgttcgcgaa atcgacttca gcgccggcga caacgccgag
    22021 gtgctgctgt tcgacctgcg ccccaacgaa ctgccgctct attgagcacc ctaatcagtc
    22081 acccaagagg aatgaccatg aaaattctgc aaatcaacgc cagtgcccgt tctgccggcg
    22141 ccaactccac ccgcctggcc gacaccatca ccgcccgcct gcaggcgcag aacccggccg
    22201 tagtggtcga gctgcgcgac ctggccagca acccgcatcc ggtcctcgac gaggccgccc
    22261 tgggcgcgct gttcaccccg gccgagcagc gtactgccga acaggcagcc cgcgtggccc
    22321 tcgacgacgc gctgatcgcc caggtgcagt ccgtcgacgt catcgtcctc ggcgtgccga
    22381 tgtacaactt cggcgtgccg gtgcagctga agacctggct cgacgccatc gcccgtgctg
    22441 gcgtgacctt ccgctacacc gagaacggcc cggaaggcct gctcaagggc aagaaggtct
    22501 acgtggccct ggcccgcggc ggcatgtacc gcgatacccc ggccgactcc caggttccgt
    22561 acctgaagac catcctcgga ttcctcggcc tgaccgacgt cgagttcgtc tacgccgaag
    22621 gcctggcgat gggcgccgaa ggtgccgaca aggcctttgc tgctgctgaa gcccagatcg
    22681 gcgcgctgat tgcctgatcg acccaggcag cgggaggcgt ccggtgacgg acgcctcccc
    22741 acctctgcac cttccgcacg accttctcca gcgaacaccg ttggcgtgcc ctgttcctgg
    22801 aagagtgcgc ctgcgccgaa ccagtccccg cccggacgac cgacaaggca gcgcaattaa
    22861 gtagattcaa ggccggatag tggagaagga acgagcatgg caagcacaga ttcgagtagt
    22921 tacaccgcca ccgccaagtg gctgcattgg ggcatggcgg ccatctggat catcagctgg
    22981 ggcttgggca tcctggcgac tcactggcgc gacgagctga atcccgagca tggcctgacc
    23041 atcctgcaca aggcgctggc cagtaccctg ctgtttctgg tcgtcgtgcg cgtggcctgg
    23101 cgtctgattc atccggctcc cgccttgccg gccagcatga gcccgctgat gcagcgtggc
    23161 gcggtcatcg ggcacgtact gctgtacgtg attgccctgg tggccctgcc gttctccggc
    23221 tggtactgga gctcggtggc ggacaagccc atcctgctgc tcggcctcgt ccagcttccg
    23281 ccgctggtgg ccccggcccc tgaccagtat gacctcgcga agatgatcca cacctggctg
    23341 ggctggttct gcggtgcgct ggtcggcggc catctgctga ttgccctcaa gcatcacttc
    23401 atcgacaggg atgcggtgct gaccggcatg ttgccgacct gcaagcgctg aacaccgggc
    23461 tcagctgttc ctgacgtcgc ctgcgaaggc tccagacgca gatgggagtc gcttccggca
    23521 tgagagcgag cctgaattta gcaactactc tggttgcagg gcgtccagca gcacagcgca
    23581 gtcgttgtgg tgaagtcgat ttgagcagat agaggatatt gccgtgagat acagccttgt
    23641 cgagagccag aaaaacgaaa tcatcctgct ggctcgtgtc cttatgatga tcctgttcat
    23701 catggcggga tggggcaagc tgaccgggtt ttcgggaacc gttggctacc tggagtccct
    23761 gggaacgcct gtccccatgc tggctgcggc gattgccgta atcatggagt tctttggggc
    23821 catcctgatc gtcgtgggct tctatacccg tccgctcgca cttctctttg cgctgttcac
    23881 cgtgggcacc gccgtgatcg ggcatagctt ctggaccatg gttgatgccg agcgggccgc
    23941 caacatgacg cagttcctca agaacatgag catcgtgggg ggcttgttgc tgttggcaat
    24001 tacgggtcca ggcaagtact cgctggacgg caagtagaca tgcgccgtgc cacgatgcgg
    24061 caggcgactg tgtcgtggca cttcccatgt gattcgcgtg tttttgcagc gaagctgaat
    24121 agtcatttgg cttccagcca ttgccgctcg ggattccgaa agcaaaattc ataacctgac
    24181 gggggaacaa ctccgacagg cttcgaggcg ttcggccttt tgcaggctgc tgccagcaat
    24241 gttggaaaac gtaaagagat cagtatgggt ttgctcgtag atggccagtg ggtcgatcag
    24301 tggtatgaca ccgagtccac cggaggaaag ttcgtccgca gcacggcgca gtttcgcaac
    24361 tgggtcacgg ccgacggacg ccctgggccg agcggcgagg gcggtttcgc ggcgcagccc
    24421 gggcgctatc acctgtatgt atcgctggcc tgcccctggg cccatcgcac gctgatcttc
    24481 cgggtactga aagggctcga agagctgatc agtgtgtcgg tggtcaatcc gtacatggcc
    24541 gagcacggct ggaccttcac gccggggccg ggtgtggtgg ccgatccggt cgggcaggcg
    24601 cgctacctgc atgagattta cctgcgtgcc tgcgccgact acagcggtcg ggtgaccgtc
    24661 ccggtactgt gggacctgca gcgcaacacc atcgtcagca acgaatcgtc ggaaatcatc
    24721 cggatgctga attcggcttt cgacggggtt ggtgccgctc ccggcgatta cgcgccggtc
    24781 gacttgttgc cgcagattga cgagatcaac gcccacatct acgacaccat caacaatggc
    24841 gtctacaagg tcggtttcgc caccgatcag caggtgtacg agcgggaggt ggtggcgcta
    24901 tttgcggcgc tcgatgccct cgaggcacgg ctatccgcgc agcgctactt gctgggtgag
    24961 cgtatcaccg aggcggactg gcgcctgttc accaccttgg tgcgctttga cgcggtctac
    25021 cacggtcatt tcaagtgcaa tctgcggcgt ctgagtggct atccgaacct gtgggcctac
    25081 atccgcgagc tctatcaatg gcctggcgta gccgctacgg tgaacttgca gcacatcaag
    25141 gaacactact accgcagcca cggcaccatc aatccgaatg gaatcgtccc gctggggccc
    25201 gtcctggacc tggcgcggcc ccatgggcgt gacttgctgg gctgacgccg attgccagca
    25261 cctttctgcg aacagagaac taaatcagcg gatcccagcg ctgcgaccag tctttttcct
    25321 ggcgagagac ctcgcgaagc agttcgcagg cctgctgcaa ctcacgggaa tcgcgttcgc
    25381 ggttgtagac cagatagatg gggtagctga actccggggc cttggtcacc cgctcgagca
    25441 ggccgcgctc caggaaggcc tgcaccaccc gagtgcggaa gtagcccgat ccgccatgtt
    25501 caagcaggta ctgcagggcc agtgggccga gattgaagct gagcgtcgcg cgggcatgct
    25561 ccggcagggc ggcgtcatgg cggcggcgga agtcctcgcc ccagtcgatg tacacgtagg
    25621 gctccgggtt gttcaccgcg cggaccagga tcagccgttc ctccagcagt ggctcgacct
    25681 gcagccccgg ccagtaggcc gggctgtgaa ccagggcggc atccaggcgg ccttggtcga
    25741 gctgctccag caactgggcg gcggcgccga tttcggcgcg gatggcatgc tgggggaatt
    25801 gctcgcgcaa gcggcccacc cattgcagca gcagcggatg gctcaggctg acctcgccgc
    25861 cgacatggag gcggtggccg tacccctcgg gcagaggcag gtcgcgctgg gcgctctccc
    25921 acgtctgcac cagctggttg gcgtaggcga tgaacgcgct gccgtcgggg gtcagcttgg
    25981 cgccagcgcg gttgcgcacg aacagcttgc tgctgagctg cgcttccaga ctctgtatcc
    26041 gcgcggtaat cgccgtctgg gtaacgtgca gacgttcagc ggcggcgacg aagctgccac
    26101 agcggacgat ctcgatgaag gtgcgggcaa gttcgatatc catgcggagg tttccagcga
    26161 aggtggctcg acgtaccggg gcaccctggc cagggtacga gatggctcgc ccgagcgatc
    26221 catgatggtg cgcagccagt cattatgtat caattattat gaactaaaga tccattatta
    26281 ttagtttgtt aacggtttca gcgggcggca gactgtcacg gccttgctcg tgcttccttg
    26341 tggggcaatc actcgaattc tgcgtgcatc tggaactgtt gatgaatcaa tggcgagaca
    26401 attcttcgcg gctgcccgca tggttgccga cggccctgca cacccggcct ctggagtgcc
    26461 tgcgcgcggc cgtgggagtg ctgctgggcg tttcggcgag tgtctggttg tgcgcctggc
    26521 tgttcgatcc ggcggtcgcg cagatgctgc ttggtccatt gggggcctcg gcggtgctgc
    26581 tgtttgcggt gtcctccggc gcgctgtctc agccttggtc gatcctcggc agctacccgc
    26641 tcgccgctct gagtggggcg gtggccgtgc agctgctcgg ggcgagcgtg gccagtgccg
    26701 tgctggcgat ggctgcctgc ctgctgacga tgttcctgct gcgctgtctt cacccgccgg
    26761 ccgcggcgct gtcgctgtgc gtggtactgg ccccggggtt gtctgcgcag ggctttgccg
    26821 tcgtactgcc ggtactgctc atcgccttca gcctgctcgc ttgcgccttg ctgttcaaca
    26881 acctgaccgg tgtgcgctat ccccgcttga gccccccggt cgggccgtcc cacctgacca
    26941 ccgatccgcc ggccgggcag cgggtgggta tcgctagcga ggacctcgac cacgccctgg
    27001 atgagttcgg tgccttcatc gatgtgaccc gtgaagacct ggcgacgctg gtccggcgta
    27061 gcgagcagca cgccctgcgg cggcagatgg gcgagctgac cgcggcacgg atcatgtcgc
    27121 gggacctgcg ctgggtgacg cccgaaacca cgctcgatca ggccctgcag attctccagt
    27181 accaccatct caaggtgctg ccggtgctgg aacgggacgt gctggtcggc gtggtgaccc
    27241 tggctgacct gatcgagctg atacgcagcc gtgctgaagg cgggctgctc ggctacctgg
    27301 gccgccgcga ggtacgggtg tccagggcga tgacccagcc ggcggtcagc gtagcggccg
    27361 acacccatgt ggttgagctg atccccctgc tgtcccgcga ggggttgcac ggactgccgg
    27421 tgctcgaagg cggcaaattg gtgggtat
//
LOCUS       DATCUN010000028         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_1090, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000028 DATCUN010000000
VERSION     DATCUN010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..>1795)
                     /locus_tag="VIO83_01055"
     CDS             complement(329..>1795)
                     /locus_tag="VIO83_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008097870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8329906.1"
                     /translation="RFADPAWQSNAFLRALLQSYLAGQSELTRFIESTDLAPLEKSRA
                     RFVAELLADAVAPSNSPLTNPAALRKLVDTGGMSLARGIRQFGDDMLNNRGLPKQVDS
                     TPFTVGENIATAKGQVVFRNEMFELLQFAPTTENVHARPLVMSPPQINKYYAIDLSPE
                     KSLIKWIQDSGVTLFVISWRNPTREHRDWGLAEYALCLDQAVDVARQITGSPDVNMWG
                     SCSGGITLAAYLGWLAARGEGHKVANTSWAVCVLDMPSALDDTTLGLFTTPSALRAAK
                     ASSRRKGVLSGQGMARMFAWMRPNDLIWNYWVNNYLLGNKPPAFDILAWNNDTTRLPA
                     QLHADYLDLVQQNPYSNPGSLEIAGESIDMTQVKVGAYVIGGTTDHITPWQGCYGTAR
                     LFGEDATYVLSNAGHLQSLVNPPGNPKSFYYAAPAAAETPETWLQNAGERQQGSWWPH
                     WREWIGQRSGESRPAPKKLGSRKYPPLCPAPGTYVMER"
BASE COUNT          279 a          551 c          620 g          345 t
ORIGIN      
        1 tggcccagcg cggctaaacc ggcgtcctgc cggtttcccc actgcgcaat gcctgcgttc
       61 ggccatcgtg gttaatgggg cgcctcagat caaaaaccaa agcgaggcgg ccttaaagcc
      121 gacctgctcc acggctgtac ctgatcccct gtgggcgagc ctgctcgcga aaaatgtccg
      181 ggcaacgctc tcatccaggc gccccgcgtc atcgttgaag tccatcgcga gcaggctcgc
      241 tcctacaaag gtccgtgtgg aacctgtggg agcgagcctg ctcgcgaaaa atgtccgggc
      301 aacgcgttca tccaggcgcc gcgcgtaatc aacgctccat gacataggtg ccaggtgcgg
      361 ggcagagggg cgggtatttg cgcgagccaa gtttcttcgg cgccggccgg ctttcgcctg
      421 agcgctggcc gatccattcg cgccaatgcg gccaccagct gccttgctgg cgctcgcccg
      481 cgttttgcag ccaggtttcc ggggtctcgg cggcggcagg tgcggcgtag tagaacgact
      541 tcgggttgcc cggcgggttc accaggcttt gcaggtggcc ggcgttggac agtacgtagg
      601 tggcgtcctc gccaaacagt cgggcggtgc cgtagcagcc ctgccagggt gtgatgtggt
      661 cggtggtgcc gccgatcaca taggcgccga ccttcacttg ggtcatgtcg atcgactcgc
      721 cggcaatctc cagggagccc gggttgctgt aggggttctg ctggaccagg tcgaggtagt
      781 ccgcgtgcag ttgggcgggt agtcgcgtgg tgtcgttgtt ccaggccagg atgtcgaagg
      841 ccggcggctt gttgcccagc aggtagttgt tgacccagta gttccagatc aagtcgttgg
      901 gccgcatcca ggcgaacatg cgggccatgc cctggccgct cagtacgccc ttgcgccggg
      961 agctggcctt ggccgcgcgc agggccgacg gggtggtgaa caaccccagg gtggtgtcgt
     1021 ccagtgccga aggcatgtcc agtacgcaca cggcccagct ggtgttggcc accttgtggc
     1081 cttcgccacg ggccgccagc cagcccaggt aagccgccag ggtgatgccg cccgagcagg
     1141 agccccacat gttgacgtcc gggctgccgg tgatctggcg tgccacgtcc accgcctggt
     1201 cgaggcacag cgcgtactcg gccaagcccc agtcgcggtg ctcgcgggtg gggttgcgcc
     1261 agctgatgac gaagagtgtc acgccgctgt cctggatcca cttgatcagg cttttctccg
     1321 gcgacaggtc gatggcgtag tacttgttga tctgcggcgg cgacatcacc aaggggcggg
     1381 catggacatt ttcggtggtg ggggcgaact gcagcagctc gaacatctcg ttgcgaaaca
     1441 ccacttggcc cttggccgtg gcgatgtttt cgccaaccgt gaacggcgtg ctgtcgacct
     1501 gttttgggag cccacggttg ttcagcatgt catcgccgaa ctggcggatg cccctggcca
     1561 ggctcatgcc cccggtgtcc accagcttgc gcagtgccgc cggattggtc agcggcgagt
     1621 tgctcggtgc cacggcatcg gccagcagtt ccgcgacgaa ccgggcgcgg ctcttttcca
     1681 gcggggcaag gtcggtggat tcgatgaagc gggtcagttc actttggccg gccaggtagc
     1741 tctgcagcag cgcgcgcagg aaggcgttgg attgccaggc tggatcggca aagcg
//
LOCUS       DATCUN010000029         3205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_151227, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000029 DATCUN010000000
VERSION     DATCUN010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151227"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VIO83_01060"
     CDS             <1..351
                     /locus_tag="VIO83_01060"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEY8329907.1"
                     /translation="AILAGIAMPAYQDYVRQSRRIDGQTALLNLQMAQERWRANNQSY
                     SASLSDLGVSTSSSENHYTIAITSGSASAIGFTATASAKSTSPQTGDTSCSTLTLGQS
                     GNQTSMTPTACWKK"
     gene            351..872
                     /locus_tag="VIO83_01065"
     CDS             351..872
                     /locus_tag="VIO83_01065"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEY8329908.1"
                     /translation="MRKRPTTGFTLIELMVTVSIMAIIAMIAAPALQMFVDKTRLKGA
                     TDRLYADMQFARSEAVKRNSSVSISFSTGSNWCYGINAGGACNCNTANACSIKTVSHS
                     EFSGITLDGATFTASSTTFNPVLGTLSAAGLARVQSAGGKQACVHMSLIGQIRLCSPA
                     GSAKLGGYPTCSC"
     gene            860..1540
                     /locus_tag="VIO83_01070"
     CDS             860..1540
                     /locus_tag="VIO83_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8329909.1"
                     /translation="MQLLKFAPRQQRGETLVGLLVGLVVGLIVLGGASLIYLNAARAN
                     STTLQLSRSNQELKAIMDVMVRDIRRAGRSGAAIHCIGGASCTDSFVGGNEDWTVAAS
                     QIDFTYDLNANGSQDSNECHGFRRVVESGIGRIEMKSDCSPTWQPLSTPNSTHISNFT
                     TTSETRCQTAGSRQLAVRKVRIYLEAQSGGVTRRMCQTIRLKNDAIVDSCSEANLSTS
                     TPFTNCPT"
     gene            1551..2696
                     /locus_tag="VIO83_01075"
     CDS             1551..2696
                     /locus_tag="VIO83_01075"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HEY8329910.1"
                     /translation="MHASLSAQSRQRGAAVLAFAVFLVFVVSLLVISSSRSLVFAQKA
                     SGNQYWAVQAHEAAQAGLEETLAWLNSVPPPASQPTNCSTLTPWVSSCWGADATTSSG
                     TDQCLSSSSGTCSNRTVTTANGFTATVTFHRDSVPLSKLNFVEIVSQAVANSDSSITA
                     TVRQVVYLSPFTGNAAAAPNAPILMQGCITQVTGTPDICPENASGGSPCTASSGTAGV
                     AIATLSTQSASSCLQTGNSVWDALFPNLSKGQLQALSQLQANAGLTRTSTPKRTVYYY
                     TPGTFVNDDHGSPSEPVIVIVECGGNCPKLNGGTDIYGMLYFDEGDSMQGWGNTTIHG
                     VFGVEGDIEKFTANTEFHYNGALDFAMSPAAPGAGVVPRIPGSWQDY"
     gene            2717..>3205
                     /locus_tag="VIO83_01080"
     CDS             2717..>3205
                     /locus_tag="VIO83_01080"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8329911.1"
                     /translation="MHDLKRMSGFSLIEALVAFLIIGVGLLGAAKMQALLSTDSAVSQ
                     QRDEAVVVAQDVIERFRGFSALPTTSGLVAYDDILSSTTAETVGGINASYSRSWTVEE
                     CCIDQSSLANACPAANCGSNAMRWKNLSVAVSWTDKGNTSNSINLTTKISRLAPAQDA
                     ALL"
BASE COUNT          642 a         1164 c          927 g          472 t
ORIGIN      
        1 gccatcctcg ccggcatcgc catgcccgct taccaggatt acgtccggca gagccggcga
       61 atcgatggcc agacggccct gctgaatctg cagatggccc aggagcgctg gcgtgccaat
      121 aaccagagct attccgccag cctcagcgat ctcggcgtaa gcacgagttc gagcgagaac
      181 cactacacca tcgccatcac cagcggcagc gccagtgcca tcggcttcac cgcaacggcc
      241 agcgccaaga gcacctcgcc gcagaccggc gacaccagct gcagcaccct gacccttggc
      301 cagtccggca atcagacctc gatgaccccc accgcctgct ggaagaaatg atgcgcaaac
      361 gaccgaccac gggtttcacc ctgatcgaac tcatggtgac cgtctcgatc atggccatca
      421 tcgccatgat cgccgcaccg gcgctgcaga tgttcgtcga caagacgcgc ctcaagggcg
      481 ccaccgatcg cctctatgcc gacatgcagt tcgcccgctc ggaggcagtc aagcgcaaca
      541 gcagcgtttc catcagcttc tcgaccggca gcaactggtg ctacggcatc aatgccggcg
      601 gcgcctgcaa ctgcaatacc gccaacgcct gcagcatcaa gaccgtcagc cacagcgagt
      661 tcagcggcat caccctcgac ggcgccacct tcaccgccag ctcgacgacc ttcaacccgg
      721 tcctgggaac cctatccgcc gcaggactgg cccgcgtgca gtccgcgggc ggcaaacagg
      781 cctgcgtgca catgagcctg atcgggcaga tccgcctgtg ctcgccggcg ggcagcgcca
      841 agctcggggg atatccgaca tgcagttgct gaaattcgca ccccgccagc agcgcgggga
      901 aaccctggtg ggcctgctgg tcggcctggt ggtcgggctg atcgtgctgg gtggggcctc
      961 gctgatctac ctcaatgccg cacgggccaa ctcgaccacg ttgcagctca gccggagcaa
     1021 ccaggaactc aaggcgatca tggacgtgat ggtgcgcgac atccgccggg ccggacgcag
     1081 cggagccgcc atccactgca tcggcggcgc cagctgcacg gacagctttg tcggcggcaa
     1141 cgaggactgg accgtggcgg ccagccagat cgacttcacc tacgacctga atgccaacgg
     1201 cagccaggac agcaacgaat gccacggctt ccgccgcgtc gtggagtctg gcatcgggcg
     1261 catcgagatg aagagcgact gctcgccgac ctggcaaccg ctgagcaccc cgaacagcac
     1321 gcacatcagc aacttcacca ccaccagcga aacccgctgc cagacagccg gcagccgcca
     1381 gctcgccgtg cgcaaggtgc gcatctacct cgaggcgcag agtggcggcg tgacccgccg
     1441 gatgtgccag accattcgcc tgaagaacga cgccatcgtc gacagctgca gcgaggcgaa
     1501 cctttccacc agtacgcctt tcaccaactg cccaacctga ggtgccatcg atgcatgcct
     1561 ccctttccgc gcagtcccga caacgtggcg cagccgtact cgccttcgcc gtcttcctgg
     1621 tcttcgtcgt cagcctgctg gtgatcagca gctcacgcag cctggtgttc gcccagaaag
     1681 cctccggcaa tcagtactgg gcggtccagg cccatgaggc agcccaagcg ggcctggagg
     1741 agaccctggc ctggctaaac agcgtgccgc cccccgccag ccagccgacc aactgcagca
     1801 cgctcacccc ctgggtgagc agttgctggg gcgcggacgc caccaccagc tccggcaccg
     1861 accagtgcct gtccagcagc agcggcacgt gcagcaaccg cacggtcact accgccaacg
     1921 gcttcaccgc cacggtgacg ttccaccgcg acagcgtccc gctcagcaag ctcaacttcg
     1981 tcgagatcgt ctcccaggca gtcgcgaaca gcgactccag catcaccgcg acggtccgcc
     2041 aggtggtcta cctgtcgccg ttcaccggca atgcggcagc cgcccccaac gcgcccatcc
     2101 tgatgcaggg ctgcatcacc caagtgaccg gtacgccgga catctgccca gaaaacgcca
     2161 gcggcggcag cccttgcacg gcatccagcg gcacggccgg agtcgccatc gccaccctga
     2221 gcacgcagag cgcctccagc tgcctgcaga ccggcaactc ggtgtgggat gcactgtttc
     2281 ccaacctgag caaaggacag ttgcaggccc tgtcgcaact ccaggccaat gcagggctga
     2341 cccgtacctc cacccccaag cgcacggtgt actactacac gccaggtacc ttcgtgaacg
     2401 acgaccacgg ttcacccagc gagccggtga tcgtcatcgt cgagtgcggc ggcaactgtc
     2461 cgaagctcaa tggcggcacc gacatctacg gcatgctgta tttcgacgaa ggcgacagca
     2521 tgcagggatg gggcaacacc accatccatg gcgtgttcgg cgttgagggc gatatcgaga
     2581 agttcacggc gaacaccgaa ttccactaca acggcgccct cgacttcgcc atgagccccg
     2641 ccgcccccgg cgcgggcgtc gtgccccgga ttccgggcag ctggcaagac tactgagagg
     2701 acaaggagtg tccacgatgc atgatctcaa acgcatgtcg ggcttcagcc tgatcgaagc
     2761 cctggtcgcc ttcctgatca tcggggtcgg cctgctcggc gccgccaaga tgcaggccct
     2821 gctctcgacc gacagcgccg tcagccaaca gcgcgacgaa gccgtggtgg tcgcccagga
     2881 cgtcatcgaa cgcttccgcg gcttttccgc cctgccgacg accagtggtc tcgtggccta
     2941 cgacgatatc ctcagcagta ccacggcgga aacggtcggc ggcatcaacg ccagctacag
     3001 ccgcagctgg acggtcgagg aatgctgcat cgaccagagc agcctcgcca acgcctgccc
     3061 ggccgccaac tgcggcagca acgcgatgcg ctggaagaac ctgtcggtcg ccgtatcctg
     3121 gacggacaag ggcaacacct cgaacagcat caacctgacc accaagatat cccgccttgc
     3181 cccggcccag gatgccgcct tgctg
//
LOCUS       DATCUN010000030         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_245215, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000030 DATCUN010000000
VERSION     DATCUN010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245215"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..1481)
                     /locus_tag="VIO83_01085"
     CDS             complement(258..1481)
                     /locus_tag="VIO83_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8329912.1"
                     /translation="MPKPACFATRPVRCLILAALLGCLAVTPLQARQDGKSRSEQAEA
                     KQKLAELRHKMEALAKEQADTAARRDSANAELAKQANALAGAARAVRQTDAELAAKQQ
                     QLDQLQQQRTALRQTLDSQRAAIADLLRATYALGHGSDLRLLLGDEDVARIARALVYS
                     KYFQQDRVQRVQRLMADLAKLQELEDAITAEQQALQATRTQREQQAAVLARQRAAQQK
                     LAAETDAQYKDQAQRLAALKQNAQSLNNLLDKLQKAIDEAAARAAAERATAVRKHPGH
                     KPPPPVEVGGASANIRGNLPWPASGVVNSYGNGVLIKASGGSEIHAVARGRVIYAGFL
                     RGYGMLLIVNHGNGWMSMYGNNETLLHDVGDQVEAGQAIGTASPPTGVNTGVYFELRR
                     NNQPVDPRSWLSQRR"
     gene            1950..>2238
                     /locus_tag="VIO83_01090"
     CDS             1950..>2238
                     /locus_tag="VIO83_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329913.1"
                     /translation="MAASGSATLYDPAFEHDSCGFGLVAQIDGRASAALVDTAFAALA
                     KLSHRGGVNADGISGDGCGVLIHRPADWLRVLAAADGIVPHERFAAGLVFLD"
BASE COUNT          286 a          738 c          813 g          401 t
ORIGIN      
        1 cgccgccacg ctggcggcag cggccggtgc gctctgcgcg cggacatccg gcgcggccag
       61 caggaacgga acggtcagca gcagaactgc catgccgatc gtcgggctag agggggaaag
      121 acgcatgcga gtgactccgt acagcagaca gcgccgcgcc ggagcgcgac ggaaaatgtg
      181 gtcaggcatg agacaatcag atgccggggt aattcagcgt gaattcgttc ctagcctgcg
      241 atgcggctgc gttcgcgtca acggcgctgg ctcagccagc tgcgcggatc gaccggctgg
      301 ttgttccggc gcagctcgaa atagaccccg gtgttgacgc cggtgggcgg gctggcggtg
      361 ccgatcgcct ggccggcctc gacctggtcg ccgacgtcgt gcagcagggt ctcgttgttg
      421 ccgtacatgc tcatccagcc gttgccgtgg ttgacgatca gcagcatgcc gtagccgcgc
      481 aggaagccgg cgtagatcac ccggccgcgc gccaccgcgt ggatctcgct gccgccgctg
      541 gccttgatca gcacaccgtt gccatagctg ttgaccacgc cgctggccgg ccatggcagg
      601 ttgccgcgga tgttggcgct ggcgccgccg acctcgaccg gcggtggcgg cttgtgtccg
      661 gggtgcttcc tgaccgctgt ggcacgttcg gcggcggcgc gggcggcggc ttcgtcgatc
      721 gccttctgca gcttgtcgag caggttgttg agcgattgcg cgttctgctt cagcgcggcc
      781 agccgctgcg cctggtcctt gtactgggcg tcggtctcgg ccgccagctt ctgctgcgcg
      841 gcgcgctgtc tggccagcac ggccgcttgc tgctcgcgct gggtccgggt cgcctgcaga
      901 gcctgctgtt cggcggtgat cgcatcctcc agttcctgca gtttggccag gtcggccatc
      961 aatcgctgca cgcgttgcac gcggtcctgc tggaagtatt tcgagtacac cagcgcgcgc
     1021 gcgatgcgcg ccacgtcctc gtcgcccagc agcaggcgca ggtccgagcc gtggccgagc
     1081 gcataggtgg cgcgcagcag gtcggcgatc gcggcgcgct ggctgtccag cgtctgcctc
     1141 agcgcggtgc gctgttgctg cagttgatcc agctgctgct gcttggcagc cagctcggca
     1201 tcggtctggc gcaccgcgcg tgccgcaccg gccaacgcgt tggcctgttt ggccagctcc
     1261 gcgttggcgc tgtcgcgccg ggcggcggta tcggcctgct ccttggccag cgcctccatc
     1321 ttgtggcgca gctcggccag cttttgcttc gcctcggcct gctcgctccg gctcttgccg
     1381 tcctggcgcg cttgcagcgg agtgacggcc aggcagccca gcaatgcggc gaggatcagg
     1441 cagcggacgg ggcgggtggc gaagcaggcg ggtttgggca tgcggctgat tatccccgag
     1501 cggggcgggc ggagcgagac ggcgctcatg tccggctgta ccggatgtcg ggggcgcagt
     1561 ggccgcgtcg gtggcgaggt ctcctccacg gtgcgccata tgcctgaaaa tgccgcccca
     1621 cgaggcactt gaacgcagct cggcggggtg tcgagccgcg cttgcggaag gccttccccg
     1681 cgcgcccgcc gttggctgtc tgcttcgtca tggctcgatc caggttcgcc ggctgcccgg
     1741 tggccgacct ggcggttccg gctgaaaagt gcagctcggc gcgccggcct cgccagggcc
     1801 gcgcccgacg gcggtcgaca gatggaagtt tagccgtcca aacaaatatt ttgacgcggc
     1861 gcagcaattg tgttttagtc ggagccgctc ctgcgcaaaa cgattggtgc catcggcatc
     1921 gcggcgacct gcgcagcccg gaggggtaga tggcggcaag cggctcggcg acgttgtacg
     1981 acccggcttt cgaacacgac agctgcggct tcggcctggt tgcgcagatc gacgggcgcg
     2041 ccagtgccgc gctggtcgac accgcgtttg ccgcgctggc gaaactctcc catcgcggcg
     2101 gcgtgaacgc cgacggcatc agcggcgacg gttgcggcgt gctgatccat cgcccggcgg
     2161 actggctgcg cgtgctggcg gcggccgacg gcatcgtgcc gcatgaacgc ttcgcggcgg
     2221 ggttggtgtt cctcgacc
//
LOCUS       DATCUN010000031        17686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_57441, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000031 DATCUN010000000
VERSION     DATCUN010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 17686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17686
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57441"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VIO83_01095"
     CDS             <1..502
                     /locus_tag="VIO83_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003193164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY8329914.1"
                     /translation="LAFYGMSTFEGPMMAIKTVNALSHYTDWTIGHVHAGALGWVAMV
                     SIGSLYHLIPKVFGREQMHSIGLINAHFWLATIGTVLYIASMWVNGIAQGLMWRAVNE
                     DGTLTYSFVEALEASHPGFIVRAIGGAFFLIGMLLMAYNTWRTVRVAKAAEIEAAEKF
                     VVEGAH"
     gene            502..1113
                     /gene="ccoO"
                     /locus_tag="VIO83_01100"
     CDS             502..1113
                     /gene="ccoO"
                     /locus_tag="VIO83_01100"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEY8329915.1"
                     /translation="MNKHEIIEKNIGLMALLMVIAVSIGGLTQIVPLFFQKETTQPVE
                     GLKPYTALQLEGRDIYIREGCVSCHSQMVRPFRAETERYGHYSVAGESVYDHPFLWGS
                     KRTGPDLARVGGRYSDDWQRAHLYNPRNVVPESKMPAYPWLVEHKLDGKDTAAKMKAL
                     KSVGVPYTQEDMAGARDAVKGKTEMDALVAYLQVLGTSLKNKR"
     gene            1120..1305
                     /locus_tag="VIO83_01105"
     CDS             1120..1305
                     /locus_tag="VIO83_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEY8329916.1"
                     /translation="MDIGTLRGLGTALVLVAFVGLLIWAFSGRRKKGFDEAANLPFAD
                     EPKSEKREEKASGSKHE"
     gene            1302..2216
                     /gene="ccoP"
                     /locus_tag="VIO83_01110"
     CDS             1302..2216
                     /gene="ccoP"
                     /locus_tag="VIO83_01110"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEY8329917.1"
                     /translation="MTSFWNWYITILSLGTLLALTWLIFATRKGQRPDTTDQTMGHTF
                     DGIEEYDNPLPRWWFMLFVGTLIFSVAYLILYPGLGNWKGVLPGYAEGWTGVNQWKKE
                     MARADEQYGPLYAKFAAMPVAEVAKDEQALKMGARLFASNCSVCHGSDAKGAYGFPNL
                     TDHDWLYGGQADTIKTTIMGGRQAAMPAWKDILGEEGIKNVAGYVRSLSGLKNPEGLD
                     IAAGQKTFATNCAACHGAEGKGTQAMGAPNLTDGTWLYGSSFAQVQQTLRFGRNGMMP
                     AQKDMLGNDKVHLLAAYVYSLSQKAPQQ"
     gene            2456..3874
                     /gene="ccoG"
                     /locus_tag="VIO83_01115"
     CDS             2456..3874
                     /gene="ccoG"
                     /locus_tag="VIO83_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEY8329918.1"
                     /translation="MSEKIPVQDVTPPSPAKKGESVDLYAKREMIYTKAFTGLFRNIR
                     MVSGAFLFLMFFGTAWLNWGDRQAVWWNLPERKFYIFGTTFWPQDFMLLSWLLIICAF
                     GLFFITVFAGRVWCGYTCPQSVWTWVFMWAEKVTEGDRNQRMKLDKQPMSAEKFLRKS
                     AKHAIWLGVALLTALTFVGYFTPIRQLTIDLFTLNIEGWALFWVGFFTLATYGNAGWL
                     REQVCIHMCPYSRFQSVMFDKDTLIVSYDEKRGELRGPRKKEADYKAMGLGDCIDCKM
                     CVNVCPTGIDIRNGLQIACIGCAACVDACDSIMDKMGYPRGLVGYTTEHNLSGQKTHL
                     VRPRLIGYAAALTIMMAAFATALYMRPLAELDVIKDRVLYRESQGGLIENVYTLKIMN
                     KDQRDHVYTIDVSGLDGLQLEGKREVKALAGEVLSVPLELRIEPEKLPSSANEILFTI
                     QAADIPGKTFDAESRFIGPSVR"
     gene            3889..4395
                     /locus_tag="VIO83_01120"
     CDS             3889..4395
                     /locus_tag="VIO83_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEY8329919.1"
                     /translation="MPHPEITTRWYKQFWAWFIIGILAFSVVLGLGLAYISTIKPDTL
                     ISDDYYDVGKGINQSLKREQLASRLGMHATLTLDDSAGVAELRLAGMSQPPQLVLNLL
                     SPTQAERDRRIILQPVAGEAGLYKGQMQDAVSGRRFVELIGQEGGKDWRLFDEFTLEG
                     GHAIELAP"
     gene            4407..6869
                     /locus_tag="VIO83_01125"
     CDS             4407..6869
                     /locus_tag="VIO83_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY8329920.1"
                     /translation="MAAPLPCFHCGLPVPAGSPYQTRVLGEERAMCCPGCQAVAEAIV
                     AGGLESYYRHRSETAANPEALPKALADELALYDRPDVQQGLVEHQGQQAQTCLLIEGI
                     SCAACAWLIEHHLKKLPGISEASLNLSNHRLQVAWDDARLPLSQLLAELRRIGYAAHP
                     YHADAAAEQMQRENRKALRQLGVAGLLWMQVMMAAMATWPEFNIDLSADFDAILRWTS
                     LFLTTPIVFYCCGQFFRGALRDLRTRHLTMDVSVSLAIGGAYVAGLWSTISGQGELYF
                     DAVGMFALFLLAGRYLERRARERTAQVTAQLVNLLPASCLRLDAQEHSQRILLSELQV
                     GDRVQVPPGAVIPADALILSGQSSIDESLLTGEYLPQPRTAGQRVTGGTLNIEGPLTL
                     QVEALGDDTRLSAIVRLLERAQADKPRLAELADRVAQWFLIVVLLVAAVVGVIWWQID
                     SERAFWIVLALLVATCPCALSLATPTALTTATGSLHKLGLLVTRGHVLEGLNQIDTLI
                     VDKTGTLTEGRLTLREVRALGDRDSNACLSLAAALESRSEHPIARAFGRTPLLADNVC
                     SEPGKGLQGEVDGRVLRIGEPTFVSQLAGQPAPAMPREAGQWLLLGDEQGPLAWFRLD
                     DRLRQDAPQLIDAAHARGWQVLLLSGDSSPMVASVAAELGIADARGGLTPDDKLAVLR
                     QLHEDGHRVLMLGDGVNDVPVLAGADISVAMGSATDLAKTSADAVLLSNHLDSLVQAF
                     AVARRTRRNIIENLVWACLYNGLVLPFAALGWITPGWAAIGMSLSSLVVVVNALRLSR
                     VSGSRHPVAAASAHLPATASPT"
     gene            6885..7100
                     /gene="ccoS"
                     /locus_tag="VIO83_01130"
     CDS             6885..7100
                     /gene="ccoS"
                     /locus_tag="VIO83_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEY8329921.1"
                     /translation="MSALYLLIPVAVVLVGLAIWVFFWAVDSGQYDDLDGPAHSILFD
                     DEDPKHKAGVEAATKDADGPDQEPPRG"
     gene            7093..7809
                     /locus_tag="VIO83_01135"
     CDS             7093..7809
                     /locus_tag="VIO83_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8329922.1"
                     /translation="MADLLPLLVSALILGLLGGGHCIGMCGGLMGALTLVIPAEQRQR
                     RLQLLLAYNLGRIASYTLAGLLLGTAGWALASGPAATLLRTVAGLLLIVMGLYLAGWW
                     SGLTRIEGLGRILWRRIEPLARGLLPVRNARRALLLGSLWGWLPCGLVYSTLLWAASQ
                     GSALDSALLMLAFGLGTLPVLLATGLAAERLTALLRRRGVRIAGGLLVILFGIWTLPG
                     PHQGWLMGHGSGHLSTPATN"
     gene            7898..9280
                     /gene="hemN"
                     /locus_tag="VIO83_01140"
     CDS             7898..9280
                     /gene="hemN"
                     /locus_tag="VIO83_01140"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEY8329923.1"
                     /translation="MLDAIRWDTDLIRRYDLAGPRYTSYPTAVQFHDGIGPFDLQHAL
                     RNSAQAKRALSLYVHLPFCAHICYYCGCNKVITKDRGRSAPYLQRLLREAQLVSRHLD
                     PGQVVEQLHFGGGTPTFLSHDELRQLMTGLRQHFHLLDDDTGDYSIEIDPREADWATM
                     GLLRELGFNRVSLGVQDFDPAVQRAVNRLQSLEETRSIVEAARTLQFRSINIDLIYGL
                     PLQNADSFARTVDEVIALQPDRLSVFNYAHLPERFPPQRRINAEDLPSPGVKLEMLQR
                     TIEQLSTAGYRYIGMDHFALPDDELAIAQEEGTLQRNFQGYTTHGHCDLIGLGVSSIS
                     QIGDLYSQNSSDLTAYQNSLDNKELATRRGLHCNQDDRIRRAVIQQLICHFKLDFADI
                     EGPFNIDFRGYFNEVWPRLQSCAADGLIRLDDRGIAITPAGRLLVRSVCMLFDHYLPL
                     QSTQRFSRVI"
     gene            9371..10114
                     /gene="fnr"
                     /locus_tag="VIO83_01145"
     CDS             9371..10114
                     /gene="fnr"
                     /locus_tag="VIO83_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEY8329924.1"
                     /translation="MTAGQDNIKARNLPQAHCKECSLAPLCLPLSLNMEDINALDDIV
                     KRGRPLKKGEFLFRQGDSFGSVFAVRSGALKTFSVTDGGEEQITGFHLPSELVGMSGM
                     DAEAYPVSAQALETTSLCEIPFEKLDELAIQLPQLRRQLMRLMSREIRDDQQMMLLLS
                     KKTADERIATFLVNLSARFRARGYSANQFRLAMSRSEIGNYLGLAVETVSRVFTRFQQ
                     NGLLSAEGKEVQILDAVELCALAGGRPEE"
     gene            complement(10209..10808)
                     /gene="recR"
                     /locus_tag="VIO83_01150"
     CDS             complement(10209..10808)
                     /gene="recR"
                     /locus_tag="VIO83_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY8329925.1"
                     /translation="MSFSPLIRQLIDALRILPGVGQKTAQRMALQMLERDRSGALRLA
                     HALTRAMEGVGYCRRCRTLSEDELCPQCSDPRRDDALLCVVEGPMDVFAVEQTGYRGR
                     YFVLKGHLSPLDGLGPEAIGIPELEARIGQGAFTEVILATNPTVEGEATAHYIAQMLA
                     PRGLTVSRIAHGVPLGGELELVDGGTLAHAMAGRRPMGC"
     gene            complement(10944..11270)
                     /locus_tag="VIO83_01155"
     CDS             complement(10944..11270)
                     /locus_tag="VIO83_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY8329926.1"
                     /translation="MMKGGMAGLMKQAQQMQEKMQKMQEELANAEVTGQSGAGLVSVV
                     MTGRHDVKRVSLDDSLMQEDKEILEDLIAAAVNDAVRKIEQNNQDKMAGMTAGMQLPP
                     GFKMPF"
     gene            complement(11281..13362)
                     /gene="dnaX"
                     /locus_tag="VIO83_01160"
     CDS             complement(11281..13362)
                     /gene="dnaX"
                     /locus_tag="VIO83_01160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY8329927.1"
                     /translation="MSYQVLARKWRPRSFREMVGQAHVLKALINALDNQRLHHAYLFT
                     GTRGVGKTTIARILAKCLNCEKGVSSTPCGECSICREIDEGRFVDLIEVDAASRTKVE
                     DTRELLDNVQYAPSRGRYKVYLIDEVHMLSTHSFNALLKTLEEPPPHVKFLLATTDPQ
                     KLPVTILSRCLQFSLKNMPPERVVEHLTHVLTVEQIPFEADALWLLGRAADGSMRDAM
                     SLTDQAIAFGEGKVLAADVRSMLGTLDHGQVYGVLQSLLEGDARALLEAVRHLAEQGP
                     DWAGVLAEMLNVLHRVAIAQVLPDAVDNGQGDRERVLALAAALPAEDVQFYYQMGLIG
                     RRDLPLAPDARSGFEMVLLRMLAFRPAGVPSAPDTPLKSTGISQATTDSTLAPVAGAA
                     ARIAEAPASVLPAAVIAQPAPPAAPAVQQRPAVPVVAPVAAAPREASAPVMPVDVPWD
                     TAPVNRAPAAPEERRDPSPAYVAPTAAAVQDTPAAAVASLSAVPAAGQDVAAAPTLAE
                     PPASVAQEVDDEGGSEDYDEVDVDAYAYLESMQPVDEHDLPSEPAVEPTLPSAMPATG
                     LSAEWLELFPRLGLAGMTASIAANCTLVAVDGDRWHLHLDPGQSALFNASQQRRLTDA
                     LSSYHGRPLTLQIDIVRPEQETPAQAAARQRAERQREAEASIHADPLVRQMMDQFAAV
                     IRGGTIEPLND"
     gene            complement(13466..13562)
                     /gene="ffs"
                     /locus_tag="VIO83_01165"
     ncRNA           complement(13466..13562)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VIO83_01165"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            13862..16837
                     /locus_tag="VIO83_01170"
     CDS             13862..16837
                     /locus_tag="VIO83_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8329928.1"
                     /translation="MQEDKKITLRRWMWRAFVQSALIPLILVETVLIAAYLLTNGAIR
                     DTQIEHLRQAALTDLKSAAQQEAKFVSERLKAITQLTRTYGEQVQAVLLRDAYLVDDL
                     ERQRHARTEDGVLFTRTDDGRAASFYANSTPKEKQDIDKVMRLAQLDPLMKSIHASNK
                     LVSAIYFNSWDSYNRLYPWFYTPDQYPHDMVIPSYNFYYLADATHNPQRKVNWTDIYV
                     DPAGQGWMMSALAPVYRGDFLEGVVGLDITVDNILKEIVGLQVPWNGYAMLVSGDLNI
                     MALPPSGEQDFALSELTKHSYDEAIRKEIFKPTDFNLGKRPATQALAHAISQSPSGIE
                     GINLAGKAQLAAWATIPETGWKLITVVDEAEVFSQTNALASRYQQIGYLLIAGMVLFY
                     LLFFAVMWARSRQLSDRLQQPIQKIGEMMQQIGQGNWFPPRARSHIAELERMAETTSA
                     MGAQLADSEAIRQQAQNQLELVLDSTTESIWEIDLEQRRIHIQGRFIERFGLKSSDLG
                     LDDFYARIHPDDLDAVIACHEHAEQHPDTDFTIEYRFSDACSIHHWLLSRARIVAQDA
                     SSGRILRIAGTHVDIDALKATEEALVLASQQAQAANVAKTRFLSSMSHELRTPLNAVQ
                     GFAQMIQLELPDNPNESNFKQYTDEILSASDHLCLLVDDILDLARIEAENTELRLETV
                     DARRIMSECIELIRPQASEHNLHLESHLPEGALLVQAEPRRLRQILLNLLSNAIKYNR
                     PYGHLSLSYKITAESLRLIVEDTGYGISEEKQAQLFKPFQRLGHENSNIKGTGIGLVL
                     SRELAEMMNGRLDFNSESGIGSTFWVELPFSPELQKRTRRQAPASSTGTLLRTLYVED
                     NRSSQLLVQKALADLADVDILDNGLEALHWITENPPELLLLDIDLPGLQGDSLLRSLR
                     KNPRTQDLPVIVISAGALPEDFALVSDLNVARYLTKPLKIEALREAVMRIGIQRYTPS
                     LNQSQVIGASSEGQTEIDE"
BASE COUNT         3427 a         6201 c         5155 g         2903 t
ORIGIN      
        1 cctggccttc tacggcatgt ccaccttcga aggcccgatg atggccatca agaccgtcaa
       61 cgccctgtcc cactacaccg actggaccat cggccacgta cacgccggcg ccctcggctg
      121 ggttgccatg gtgtccatcg gctccctgta ccacctgatc ccgaaagtgt tcggccgcga
      181 gcagatgcac agcatcggcc tgatcaacgc ccacttctgg ctggccacca tcggcaccgt
      241 gctgtacatc gcctccatgt gggtcaacgg tatcgcccag ggcctgatgt ggcgtgcagt
      301 caacgaagac ggcaccctga cctactcctt cgttgaagcc cttgaagcca gccacccggg
      361 cttcatcgtc cgcgctatcg gcggcgcctt cttccttatc ggcatgctgc tgatggccta
      421 caacacctgg cgcaccgtgc gtgttgccaa ggcggcggaa atcgaagccg cagagaagtt
      481 cgtggttgaa ggagctcact gatgaacaag cacgaaatca tcgagaagaa catcggcctg
      541 atggcgctgc tgatggtcat cgccgtcagc atcggcggtc tgactcagat cgtcccgctg
      601 ttcttccaga aggaaaccac ccagccggtc gagggcctca agccctacac cgcgctgcag
      661 ctagaaggcc gtgacatcta catccgtgag ggctgtgtca gctgccactc gcagatggtg
      721 cgtccgttcc gcgccgaaac cgagcgctac gggcactact cggtcgctgg tgaaagcgtc
      781 tatgaccatc cgttcctgtg gggctccaag cgtaccggtc cggacctggc tcgcgtcggc
      841 ggccgctact ccgacgactg gcagcgcgcc cacctgtaca acccgcgcaa cgtagtgccc
      901 gagtcgaaga tgccggccta cccgtggctg gttgagcaca agctcgacgg caaggacacc
      961 gccgccaaga tgaaggcact gaagtccgtg ggcgtgcctt acacccagga agacatggcc
     1021 ggggcccgtg acgcagtcaa gggcaagacc gaaatggacg cgctggtcgc gtacctgcag
     1081 gtcctcggca ccagcctcaa gaacaaacgg taattcgcta tggatatcgg gactctgcgt
     1141 ggtctgggca ccgccctggt actggtcgca ttcgtaggcc tgctgatctg ggcctttagc
     1201 ggcagacgca agaaaggctt tgacgaggcg gccaacctgc ccttcgccga tgagcccaaa
     1261 tccgaaaagc gtgaagaaaa agcttctggg agtaagcacg aatgaccagt ttctggaatt
     1321 ggtacatcac catactcagc ctcggcaccc tgctggcgct gacttggctg atcttcgcga
     1381 cccgcaaggg acagcgccct gacaccaccg accagaccat ggggcacacc ttcgacggca
     1441 tcgaggagta cgacaatccg ctgccgcgct ggtggttcat gctgttcgtc ggcaccctga
     1501 tcttctccgt cgcttacctg atcctctacc cgggtctggg caactggaaa ggcgtcctgc
     1561 cgggttacgc ggaaggctgg accggtgtca accagtggaa gaaggagatg gcccgcgccg
     1621 acgagcaata cggcccgctg tatgccaagt tcgccgccat gccggtggcc gaggtagcca
     1681 aggacgaaca ggccctgaaa atgggtgcac gcctgttcgc ttccaactgc tccgtgtgcc
     1741 acggctccga cgccaagggc gcctacggct tcccgaacct gaccgaccat gactggctgt
     1801 atggtggcca agccgacacc atcaagacca ccatcatggg cggtcgtcag gcggcgatgc
     1861 cggcctggaa ggacatcctc ggcgaggaag gcatcaagaa cgtcgctggc tatgtgcgca
     1921 gcctgtccgg cctgaagaac cccgaaggct tggacatcgc ggctggccag aagaccttcg
     1981 ccaccaactg cgcggcctgc cacggcgccg aaggcaaggg cacccaggcc atgggcgcac
     2041 cgaacctcac cgacggcacc tggctgtacg gctccagctt tgcccaggtt cagcagaccc
     2101 tgcgctttgg ccgtaacggc atgatgccgg cgcagaagga catgctgggc aacgacaagg
     2161 tgcacctgct tgctgcctac gtgtacagcc tgtcgcagaa ggcaccgcag cagtaacgct
     2221 ctctgctcgg tcccaaaacg ccgctttccg ctcgccggaa agcggcgttt tgctttctag
     2281 catcagcgcc agccctcgac acagcataaa gacggccatg ccgccaccgc gcttacgcga
     2341 cctagcgtcg catgcagatg agcggcatta ggcgtaacat tatcgagaag gtgaaatgat
     2401 cccagccggc catgtaccga ccggggcgcc cattcccaag ctgtggtaga gaccgatgag
     2461 cgagaagatc cccgtccagg atgtaacccc cccgtccccc gccaagaaag gcgaaagcgt
     2521 cgatctctac gccaagcgcg agatgatcta caccaaggcc ttcactggcc tgttccgcaa
     2581 catccgcatg gtcagcggtg ccttcctgtt cctgatgttc ttcggaaccg cctggctcaa
     2641 ctggggcgac cgccaggccg tctggtggaa cctgcctgaa cgcaagttct atatcttcgg
     2701 caccaccttc tggccgcagg acttcatgct gctgtcctgg ctgctgatca tctgcgcctt
     2761 cggcctgttc ttcatcaccg tatttgccgg ccgcgtctgg tgcggctata cctgcccgca
     2821 gagcgtatgg acctgggtgt tcatgtgggc ggaaaaggtc accgagggtg atcgcaacca
     2881 gcgcatgaag ctcgacaagc agccgatgag cgccgagaaa ttcctgcgca agagcgccaa
     2941 gcacgcgatc tggctgggcg tggccctgct cacggcactg accttcgtcg gctacttcac
     3001 cccgattcgc cagctgacca tcgacctctt caccctcaat atcgaaggct gggccctgtt
     3061 ctgggtcggc ttcttcaccc tggccaccta cggcaacgcc ggctggctgc gcgagcaggt
     3121 gtgcatccac atgtgcccct actcgcgctt ccagagcgtg atgttcgaca aggacaccct
     3181 gatcgtctcc tacgacgaga aacgtggcga gctgcgcggt ccgcgcaaga aggaagccga
     3241 ctacaaggcc atgggcctcg gtgactgcat cgactgcaag atgtgcgtga acgtctgccc
     3301 gaccggcatc gacatccgca acggcctgca gatcgcctgc atcggctgcg ccgcctgcgt
     3361 cgacgcctgc gacagcatca tggacaagat gggctatccg cgaggactgg tcggctatac
     3421 caccgagcac aacctctccg gccagaagac ccacctcgtg cggccgcgcc tgatcggcta
     3481 tgccgccgcc ctgaccatca tgatggccgc cttcgccacc gcgctgtaca tgcgtccgct
     3541 ggccgaactc gacgtcatca aggaccgcgt gctctatcgc gaaagccagg gcggcctgat
     3601 cgagaacgtc tacacgctga agatcatgaa caaggatcag cgcgaccacg tctacaccat
     3661 cgacgtctcc ggcctcgatg gcctgcagct cgaaggcaag cgcgaggtca aggctctggc
     3721 tggcgaagta ctctccgtgc cgctcgagct gcgcatcgaa ccggaaaaac tgccttccag
     3781 cgccaacgag attctcttta caattcaggc cgccgacatt cccggcaaaa ccttcgacgc
     3841 cgaaagccgc ttcatcggcc caagcgtccg ctgagataga gagtgagcat gccacacccc
     3901 gagatcacca cccgttggta caagcagttc tgggcctggt tcatcattgg catcctggcc
     3961 ttctcggtgg ttctcgggct ggggctggcc tacatctcca cgatcaagcc cgacaccctg
     4021 atttccgacg actattacga cgtcggcaag ggcatcaacc agtcgctcaa gcgtgagcag
     4081 ttggcctcgc gcctgggcat gcacgccacc ctcaccctcg acgacagcgc cggcgtggca
     4141 gagctgcgcc tggctggcat gagccagccg ccacagctgg tgctcaacct gctctcgccg
     4201 acccaggcgg agcgcgaccg ccgcatcatc ctgcaaccgg tcgccggtga agccgggctg
     4261 tataaaggcc agatgcagga tgccgtcagc ggtcgccgct tcgtcgagct gattggccag
     4321 gagggcggca aggactggcg cctgttcgat gagttcaccc tggaaggcgg ccacgccatc
     4381 gaactcgcgc cctgacggtt cccgacatgg ccgctcccct gccctgcttt cactgcgggc
     4441 tgccggtacc tgccggcagc ccctatcaga cccgcgtgct cggcgaagag cgggccatgt
     4501 gctgtccagg ctgccaggcg gtggccgagg ccatcgtcgc cggcgggctg gaaagctact
     4561 atcgccaccg cagcgaaacc gccgccaacc ccgaggcact gcccaaggcg ctcgccgacg
     4621 agctggccct gtatgatcgc ccggatgttc agcaaggcct ggtggaacac cagggacagc
     4681 aggcgcagac ctgcctgctg atcgagggca tcagctgtgc ggcctgtgcc tggctgatcg
     4741 aacaccacct gaagaaactg cccggcatca gcgaggccag cctcaacctg tccaaccatc
     4801 gcctgcaggt ggcctgggat gatgcccgcc tgccgctcag ccagttgctg gccgagctgc
     4861 gccggatcgg ctatgccgcc catccctacc acgcagacgc ggcggccgaa cagatgcagc
     4921 gggaaaaccg caaggcgctg cgccagctcg gcgtggccgg cctgctgtgg atgcaggtga
     4981 tgatggccgc catggccacc tggcccgagt tcaacatcga cctcagcgcc gacttcgacg
     5041 ccatcctgcg ctggaccagc ctgttcctca ccacgcccat cgtcttctac tgctgcggcc
     5101 agttcttccg cggcgccctg cgcgatctgc gcactcgcca cctgaccatg gacgtttcag
     5161 tatcgctggc catcggcggc gcctatgtcg ccggtctgtg gtcgaccatc agcgggcagg
     5221 gcgagctgta cttcgacgcc gtcggcatgt tcgccctgtt cctgctcgcc ggtcgctacc
     5281 tggagcggcg cgcccgcgag cggacggccc aggtcaccgc ccagctggtc aacctgctgc
     5341 cggcctcctg cctgcgcctg gacgcccagg aacacagcca gcgcatcctg ctcagcgaac
     5401 tgcaggtcgg cgaccgcgtg caggttcccc ccggcgcggt cattccggcc gatgccctga
     5461 tcctcagcgg ccaatcgagc atcgacgaat cgctgctgac cggcgaatac ctgccgcagc
     5521 cgcgcaccgc cggccagcgc gtcaccggcg gcacgctgaa catcgaggga ccgctgaccc
     5581 tgcaggtcga ggcactgggt gacgataccc gcctctccgc catcgtccgc ctgctcgagc
     5641 gcgcccaggc cgacaagccg cgcctggccg aactggccga ccgggtcgcc cagtggttcc
     5701 tgatcgtggt cctgctggtc gccgccgtgg tcggcgtgat ctggtggcag atcgacagcg
     5761 aacgggcctt ctggatcgtc ctcgccctgc tggtcgccac ctgcccctgt gcgctatcgc
     5821 tggccacgcc gaccgcactg accaccgcca ccggaagcct gcacaagctc ggcttgctgg
     5881 tcacccgcgg ccacgtgctg gagggcctga accagatcga caccctgatc gtcgacaaga
     5941 ccggcacgct caccgaagga cgcctgaccc tgcgcgaagt gcgggcgctg ggcgatcggg
     6001 acagcaatgc ctgcctgagc ctcgccgccg cgctggaaag ccgctcggaa catccgattg
     6061 cccgcgcctt cgggcgcacg ccactgctcg ccgacaacgt ctgcagcgag cccggcaagg
     6121 gcctgcaggg cgaagtcgac ggccgtgtgc tgcgcatcgg cgagccaacg ttcgtcagcc
     6181 agctcgccgg gcaaccggcc cccgccatgc ctcgggaagc cggccagtgg ctgctgctcg
     6241 gcgatgaaca gggcccgctg gcctggttcc gcctggacga ccgcctacgc caagacgccc
     6301 cgcaactgat cgacgccgcc cacgcgcgcg gctggcaggt gctgctgctc tccggcgaca
     6361 gctcgccgat ggtcgccagc gtcgccgccg aactgggcat cgccgacgcc cgcggcggac
     6421 tcacgccgga cgacaagctc gccgtcctgc gtcagctgca cgaggacggc caccgcgtgc
     6481 tgatgctcgg tgacggcgtc aacgacgtgc cggtgctggc cggcgccgat atcagcgtgg
     6541 ccatgggcag cgccaccgac ctggccaaga ccagcgccga cgccgtgctg ctgtccaacc
     6601 acctcgacag cctggtccag gccttcgccg tggcgcggcg cacccgccgc aacatcatcg
     6661 agaacctggt ctgggcctgc ctgtacaatg gcctggtgct gccgttcgcc gccctcggct
     6721 ggatcacccc aggctgggcc gccatcggca tgtcgctgag ctccctggtg gtggtggtca
     6781 acgccctgcg cctgagccgg gtatccggca gccgccatcc ggtcgcagcc gccagcgctc
     6841 acctgccggc cactgccagc cccacctgac ccggagacgc ctacatgtcc gcactctacc
     6901 tgttgatccc ggtcgccgtg gtgctggttg gcctggccat ctgggtattc ttctgggccg
     6961 tcgacagtgg ccagtacgac gacctcgacg gcccggcgca cagcatcctg ttcgacgacg
     7021 aagaccccaa gcacaaagcc ggcgtcgaag ccgcgaccaa ggatgccgac ggccccgacc
     7081 aggaaccgcc gcgtggctga tctgctgccc ctgctggtgt ccgcgctgat cctcggcctg
     7141 ctcgggggtg gccactgcat cggcatgtgt ggcggtctga tgggcgccct gaccctggtc
     7201 attcccgccg aacagcgcca gcggcgcctg caactgctgc tcgcctacaa ccttggccgc
     7261 atcgcctcct ataccctggc cggcctgctc ctcggcaccg ccggctgggc cctggccagc
     7321 gggccggccg ccaccctcct gcgcaccgtc gccggcctgc tgctgatcgt catgggcctg
     7381 tacctggccg gctggtggag cggcctgacc cgcatcgaag gcctcggccg catcctctgg
     7441 cgacgcatcg agccgctggc ccgcggcctg ctgccggtac gcaacgccag gcgcgccctg
     7501 ctgctcggca gcctgtgggg ctggctgccc tgcggcctgg tctacagcac cctgctgtgg
     7561 gctgccagcc agggcagcgc cctggacagc gccctgctga tgctggcctt cggcctaggc
     7621 accctgccgg tgctgctggc tacgggcctc gcggccgaac gcctgaccgc cctgctgcgc
     7681 cgtcgcgggg tgcgtatcgc cggcggcctg ctggtgatcc tgttcggcat ctggacgctg
     7741 cccggcccgc accagggctg gctgatgggc cacggcagcg gccacctcag cacgcctgcg
     7801 accaactgaa gcggttgatg caagtcaaca cccccctggc gccccggcct tagactgaaa
     7861 caactgaaac ataagtctga taatccggga tacccgcatg ctcgacgcca tccgctggga
     7921 taccgacctg atccgtcgct acgatctggc cgggccgcgt tacacctcct atcccaccgc
     7981 cgtgcaattc cacgacggta tcgggccgtt cgacctccaa cacgccctgc gcaacagtgc
     8041 ccaagccaaa cgcgccctgt cgctctacgt gcacctgccg ttctgcgcgc acatctgcta
     8101 ctactgcggc tgcaacaagg tcatcaccaa ggaccgcggc cgcagcgcac cctacctgca
     8161 gcgcctgctg cgggaagcgc agctggtcag ccgccatctc gatcccggcc aggtggtcga
     8221 acagttgcac ttcggcggtg gtaccccgac cttcctcagc cacgacgaac tacgccagct
     8281 gatgaccggg ctgcgccagc acttccacct gctggacgac gataccggcg actacagcat
     8341 cgagatcgac ccgcgcgaag ctgactgggc gaccatgggc ctgctgcgcg agctcggttt
     8401 caaccgcgtc agcctcggcg tgcaggattt cgatcccgcc gtgcaacggg ccgtcaatcg
     8461 cctgcaaagc ctcgaggaaa cccgctccat cgtcgaggcc gcgcgcaccc tgcagttccg
     8521 ctcgatcaac atcgacctga tctatggcct gccgctgcaa aacgccgact ccttcgcccg
     8581 cacggtcgac gaagtcatcg ccctgcagcc cgaccgcctc tcggtgttca actacgccca
     8641 cctgcccgag cgctttccgc cgcagcggcg gatcaacgcc gaggatctgc ccagcccagg
     8701 cgtcaagctg gagatgctcc agcgcaccat cgagcagctg tccaccgcgg gctaccgcta
     8761 tatcggcatg gaccacttcg ccctgcccga cgacgaactg gccatcgccc aggaagaagg
     8821 cacgctgcag cgcaacttcc agggctacac gacccacggc cactgcgacc tgatcggcct
     8881 aggcgtgtcc tcgatcagcc agatcggcga cctctacagc cagaactcca gcgacctgac
     8941 cgcctaccag aacagcctgg acaacaagga gctggccacc cgccgcggcc tgcactgcaa
     9001 ccaggatgac cgcatccgcc gcgccgtgat ccagcagctg atctgccact tcaagctcga
     9061 cttcgccgac atcgaagggc cgttcaacat cgatttccgc ggctacttca acgaagtgtg
     9121 gccacgcctg cagtcctgcg cggccgacgg cctgatccgc ctggacgacc ggggtatcgc
     9181 catcaccccc gccgggcgtc tgctggtccg ttcggtatgc atgctgttcg accactatct
     9241 gcccctgcag agcactcaac gcttctcccg agtcatctga ccgcacgttc ggcgtagtgg
     9301 cacgctggca gccggtagca cgcttggggt aaccttgcgc gcataccagc tgtgtacgta
     9361 aggattcgcc atgaccgccg gccaagacaa cataaaggcg cgcaacctgc cgcaagcgca
     9421 ctgcaaggag tgcagcctcg ccccgctgtg cctgccgctg tccctgaaca tggaggacat
     9481 caacgcgctg gacgacatcg tcaagcgcgg ccggccgctg aagaaaggcg aattcctgtt
     9541 ccgccagggc gacagcttcg gttcagtgtt cgccgtgcgc tccggcgcgt tgaagacctt
     9601 cagcgtcacc gacggcggcg aggagcagat caccggcttc catctgccca gcgaactggt
     9661 cggcatgtcc gggatggatg ccgaggccta tccggtctcg gcccaggcgc tggaaaccac
     9721 ttcgctgtgc gagatcccgt tcgagaagct cgatgaactg gccatccagc tgccgcagct
     9781 gcgtcgtcag ctgatgcggc tgatgagccg cgaaatccgc gacgaccagc agatgatgct
     9841 gctgctgtcg aagaaaaccg ccgacgagcg catcgccact ttcctggtca acctctccgc
     9901 gcgcttccgc gcccgcggct actcggccaa ccagttccgc ctggccatgt cgcgcagcga
     9961 gatcggcaac tacctgggcc ttgccgtgga aaccgtatcg cgggtattca cccgcttcca
    10021 gcagaacggc ctgctcagcg ccgaaggcaa ggaagtgcag attctcgacg ctgtcgagct
    10081 gtgcgccctg gctggcggac gccccgagga gtgacctcgc gacacccgca gcccgtgcaa
    10141 atgcaacgcc cgcctcaggc ctcctgaggc gggcgttttt tcaacggcct gagcgggctg
    10201 ccgcgaactc agcagcccat cggccggcga ccggccatgg cgtgggccag ggtgccgccg
    10261 tcgaccagtt ccagctcgcc gcccagcggc acgccatggg caatgcgcga caccgtcagg
    10321 ccacgcggcg ccagcatctg ggcgatgtaa tgggccgtgg cctcaccctc caccgtcgga
    10381 ttggtggcga ggatcacctc ggtgaaggcg ccctgcccga tccgcgcctc cagctccgga
    10441 atgccgatgg cttccgggcc aagaccatcc agcggcgaca gatgcccctt gagcacgaaa
    10501 tagcgaccgc gatagccggt ctgctcgacg gcgaacacgt ccatcggccc ctccaccacg
    10561 cacagcaacg cgtcgtcgcg gcgcggatcg ctgcactgcg ggcacagctc gtcctcgctg
    10621 agcgtgcggc agcgccggca gtaaccgacc ccttccatcg cccgcgtcag cgcatgcgcc
    10681 agacgcagcg caccgctacg atcacgctcg agcatctgca gcgccatgcg ctgggcagtc
    10741 ttctgtccga cgccgggcag gatacgcagg gcgtcgatca gttggcgaat cagggggctg
    10801 aagctcatgg cgagataccg atgacacaaa ccgaaaaggg agaggcgact tcctgcggca
    10861 gaagaacccg cccgtaggag caaatttatc tgcgatcaac tgcatcgcga ataaattcgc
    10921 ccctacggtc aggccggaga cactcagaac ggcatcttga agcccggcgg caactgcatg
    10981 cccgcggtca tgccggccat cttgtcctgg ttgttctgct cgatcttgcg cacggcatcg
    11041 ttcaccgcgg cagcgatcag gtcctcgagg atttccttgt cttcctgcat caggctgtca
    11101 tcgagggaga cgcgcttgac gtcgtggcga ccggtcatca ccacgctcac cagcccggcg
    11161 ccggactgac cggtcacctc ggcattggcc agctcttcct gcatcttctg catcttttcc
    11221 tgcatctgct gggcctgctt catcagaccg gccatgccac ctttcatcat gggaatcacc
    11281 tcaatcgttc aggggttcaa tcgtgccgcc gcggatcacc gcggcgaatt ggtccatcat
    11341 ctgccgaacc agtggatcgg catgaatcga tgcctcggcc tcgcgctgtc gctccgcacg
    11401 ctggcgggca gcggcctggg ccggcgtttc ctgctcgggg cggacgatgt cgatctgcaa
    11461 ggtcaacggc cgcccatgat agctgctcag cgcatcggtc agacgacgct gctgactggc
    11521 gttgaacagc gcgctttgcc ccggatccag atgcagatgc cagcggtcgc cgtccaccgc
    11581 caccagcgta cagttggccg cgatgctcgc ggtcatgccc gccaggccca gccggggaaa
    11641 cagctccagc cattcagccg acagtcccgt ggcgggcatg gcggacggca acgttggctc
    11701 gacggccggc tcgctcggca gatcatgctc gtccaccggc tgcatgcttt cgagataggc
    11761 gtaggcatcc acgtctacct cgtcgtaatc ctcactgccg ccctcgtcat caacctcctg
    11821 cgccacgcta gcaggcggct cggccagcgt aggcgcagca gcaacatcct ggcctgctgc
    11881 aggaaccgcg cttagcgagg cgactgccgc ggctggcgtg tcctgtacgg cggcggcagt
    11941 gggcgccacg tacgcgggag acggatcccg acgctcctcc ggcgccgcag gcgcacgatt
    12001 cacgggagcg gtatcccagg gcacgtccac cggcatgacc ggcgcgctcg cttccctggg
    12061 cgccgcggca accggcgcaa cgactggcac cgccgggcgc tgttgaactg cgggcgcggc
    12121 aggcggcgca ggctgtgcga tcactgccgc cggcagtaca gaagccggag cctccgcgat
    12181 ccgcgccgca gccccggcca ccggagccag cgtggaatca gtcgtggcct gactgatccc
    12241 cgtggacttt agcggcgtgt ccggcgccga tggcacaccg gccggacgga aggccagcat
    12301 gcgcagcagc accatctcga aaccgctacg cgcatcgggc gccaacggca ggtcgcggcg
    12361 accgatcaag cccatctggt aataaaactg tacatcctcg gccggcagcg cagcggccag
    12421 ggccagcacc cgctcacggt cgccctggcc gttgtcgacc gcatccggca gcacctgagc
    12481 aatcgccacg cggtgcagca cgttgagcat ctcggccagc acgccggccc agtccggccc
    12541 ctgctcggcc agatggcgca ccgcctcgag cagcgcacgc gcatccccct ccagcagcga
    12601 ctgcaacacg ccgtagacct ggccgtgatc gagggtgccg agcatcgacc gcacatcggc
    12661 ggcgagcacc ttgccctcgc cgaaggcaat cgcctgatcg gtcagactca tggcgtcgcg
    12721 catcgagcca tcggcggcgc gccccagcag ccacagcgcg tcagcctcga acggaatctg
    12781 ctcgacggtc agcacatggg tcaggtgctc gaccacccgc tccggcggca tgttcttcag
    12841 cgagaactgc aggcagcgcg aaaggatggt gaccggcagc ttttgcgggt cggtggtggc
    12901 gagcaggaac ttgacgtggg gcggcggctc ttcgagggtc ttgagcaggg cgttgaacga
    12961 gtgcgtggac agcatgtgca cttcgtcgat caggtagacc ttgtagcggc cgcggctggg
    13021 cgcgtactgc acgttgtcca gcagctcgcg ggtgtcctcg accttggtcc ggctggcggc
    13081 gtccacctcg atcaggtcga cgaaacgccc ctcgtcgatc tcccggcata tcgagcactc
    13141 gccgcacggc gtcgagctga cgcccttctc gcaattcagg cacttggcca ggatgcgtgc
    13201 gatggtggtc ttacccacgc cacgggtgcc tgtgaacaga taggcatggt gcagccgctg
    13261 attgtccagc gcattgatca gggccttgag cacatgggcc tggccgacca tttcgcggaa
    13321 cgagcgcgga cgccacttac gggcaagaac ctgataactc atcgaatcgt ttcgctggga
    13381 gcagttgagc gtggggggca atgctagcga aggacggtgc aaattgcact cgcgagggat
    13441 tttgcggggg cagaaatgga ggcggcccca ccagccacac cccggcacac gatgtcaccg
    13501 ctgtggctgc tcccttccgg gcctgaccag gttaacggct aatcattgcg gggggaccaa
    13561 tggggccacc ataacgagcc cgccattcga cgagccgcgc cattgtagcg gctcgagcga
    13621 aagttacaac cttaaccctg gaaaattctg cccacgcacc gaacaccctg atcgagtcgg
    13681 gaaaaaagat ggagcacgca atacccccgg cattttcatg tgaatctcat cacaaaaaca
    13741 gactcttgca ttatgattcc gcaccgctat gcgccaccta ttaccccgcg gcacaatcac
    13801 gacccaacaa aacggccatt ttgtccgcgc gagccccaaa cagagcgttc gagacccagg
    13861 tatgcaggaa gacaagaaaa tcaccttgcg ccgctggatg tggcgcgcgt tcgtgcaaag
    13921 cgccctgatt ccgctgatcc tggtcgagac ggtgctgatc gcggcctatc ttttgaccaa
    13981 cggcgcgatt cgcgacaccc agatcgaaca cctgcgccag gcggcactga ccgacctcaa
    14041 gagcgcagcc cagcaggaag ccaagttcgt cagcgagcgc ctcaaggcca ttacccagct
    14101 gacccggacc tacggcgagc aggtgcaggc cgtactcctg cgcgacgctt atctggtgga
    14161 cgacctcgag cgccagcgcc acgcccgcac cgaagacggc gtgctattca cccgcaccga
    14221 cgatggccgc gccgcctcct tctacgccaa tagcacgccg aaggaaaagc aggacatcga
    14281 caaggtcatg cgcctagccc agctcgatcc gctgatgaag agcatccacg ccagcaacaa
    14341 gctggtcagc gccatctatt tcaacagctg ggacagctac aaccgcctct acccgtggtt
    14401 ctacaccccc gaccagtatc cgcacgacat ggtcatcccc agctacaact tctattacct
    14461 cgccgatgcc acccacaatc cgcagcgcaa ggtgaactgg accgacatct acgtcgaccc
    14521 ggccggccag ggttggatga tgtcggcatt ggccccggtc tatcgcggcg actttctcga
    14581 gggggtggtc ggactcgata tcaccgtcga caacatcctc aaggaaatcg tcgggctgca
    14641 ggtgccctgg aacggctacg ccatgctggt cagcggcgac ctcaacatca tggccctgcc
    14701 cccttccggc gagcaggact tcgccctcag cgagctgacc aaacactcct acgacgaagc
    14761 catccgcaag gaaatcttca agcccaccga tttcaacctc ggcaagcgcc ccgccactca
    14821 agccctggcc cacgccatca gccagagccc atcgggtatc gaggggatca acctggccgg
    14881 caaggcgcaa ctggccgcct gggccaccat cccggagacc ggctggaagc tgatcacggt
    14941 cgtcgacgaa gccgaggtct tcagccagac caacgccctc gccagccgct accagcagat
    15001 cggctacctg ctgatcgccg gcatggtcct gttctacctg ctgttcttcg ccgtcatgtg
    15061 ggctcgctcg cggcagctca gcgaccgcct gcagcaaccc atccagaaaa tcggcgagat
    15121 gatgcagcag atcggccagg gcaattggtt ccctccccgc gcccgctcgc acattgccga
    15181 actggaacgg atggccgaaa ccaccagcgc catgggtgcc cagctggccg acagcgaggc
    15241 gatccgccag caggcgcaga accagctgga actggtgctc gactcgacca ccgaaagcat
    15301 ctgggaaatc gatctggagc agcggcgcat ccatatccag gggcgcttca tcgaacgctt
    15361 cggtctcaag agcagcgacc tgggactgga cgacttctac gcccgaatcc atcccgacga
    15421 cctcgacgcc gtgatcgcct gccacgaaca tgcagagcaa caccccgaca ccgacttcac
    15481 gatcgaatac cgcttcagcg atgcctgcag catccatcac tggctgctca gtcgcgcccg
    15541 gatcgtggcg caggacgcca gcagcgggcg catcctgcgg attgccggca cccatgtcga
    15601 catcgatgcg ctcaaggcca ccgaggaagc cctggtcctt gccagccagc aggcgcaggc
    15661 cgccaacgtc gccaagaccc gcttcctctc cagcatgagt cacgagctgc gcaccccgct
    15721 caacgccgtg cagggctttg cccagatgat ccagctcgaa ctgcctgata acccgaacga
    15781 aagcaatttc aagcagtaca ccgacgagat cctctccgcc agcgaccacc tgtgcctgct
    15841 ggtggacgac atcctcgacc tcgcccgcat cgaagcggaa aacaccgagc tgcgcctgga
    15901 aaccgtggat gcccgacgga tcatgagcga gtgcatcgag ctgatccgcc cccaggccag
    15961 cgagcacaac ctgcacctgg aaagccacct gccggaagga gccctgctgg tgcaggccga
    16021 accccgcagg ctgcggcaga tcctgctcaa cctgctgagc aacgccatca agtacaaccg
    16081 cccctacgga cacctgtcgc tcagctacaa gatcactgcc gaaagcctgc gcctgatcgt
    16141 cgaggacacc ggctacggca tcagcgagga aaaacaggcc cagctcttca aaccgttcca
    16201 gcgcctcggc cacgagaaca gcaatatcaa gggcacaggc atcggcctgg tgctcagccg
    16261 cgagctggcg gagatgatga acggacggct cgacttcaac agcgagtccg gtatcggcag
    16321 caccttctgg gtagaactgc cgttcagccc cgaattgcag aagcgcacac gccggcaggc
    16381 acccgccagc agcaccggca ccctgctgcg cacgctgtat gtcgaggaca accgctcgag
    16441 ccagctgctg gtccagaaag cactagcaga tctcgccgac gtggacattc tcgacaacgg
    16501 actggaggcc ctgcactgga tcaccgaaaa cccgccggag ctgctgctgc tggacatcga
    16561 cctgccggga ctgcagggcg acagcctgct gcgctccctg cgcaagaacc cccggaccca
    16621 ggacctgccg gtgatcgtca tcagcgccgg cgcgctaccg gaagacttcg ccctggtcag
    16681 cgacctcaat gtcgcccgct acctgaccaa accgctgaag atcgaggccc tgcgcgaggc
    16741 ggtgatgcgg atcggcattc agcgttacac acccagcctc aaccagtcgc aggttatcgg
    16801 cgcaagttcc gaggggcaga ccgagatcga cgagtgaacg aatgctatat gccaaggcat
    16861 atagcattcc attttggaag tttggcgagt acgtagcgac atgctcggcg ccaccacgca
    16921 gaggctgaat atattcatga catccgctac gaatattatt ttctcaagct aatcaacagg
    16981 ttgagtcaaa gcctccacac tcccccaaac tgcgaaaaac tgcccaacca caaaaaacat
    17041 cacgaaaaat caaaacaaaa aaataaaaaa tcacaccacc cacaacaata cgacgagacc
    17101 aataaatctc cgcccgcaca tacagaatca aacaaaaacc caaatatggt agaaaaatca
    17161 acaaacccaa cttgaacaac aaaaataaaa caaaacacca accatattac gaatctctta
    17221 ctggtaaaaa taagcacgca tatattttgc gtgcgtcgag ctccaaacca cccctgcgcc
    17281 atcccccgcg agaaagtagc caagcccctg ttttaaaagc aaaaactcac tactcaggcg
    17341 caactatcac gcccgctcaa agcccatccc tcatgcctga tcggaagtcc tgacagactt
    17401 cattacccct cgagtgatgc cctcatcccc ttccccctca catatccatg cttcccaagc
    17461 agaatagtct agcgcacgtc aaaaagcagg ccaatcacgg cgccgacaca aaaaggcaac
    17521 accaaggcgt cgactttacg actcgaaaag ccgcactaca ctttcgacca caggcacacc
    17581 atccgctatt ccctgatggt gaagccgtta actctcaaga aatctcgtat tcaagaaaca
    17641 acggaaaatc ttggggcggt agcgtgcctg aatgacgttg tcaaac
//
LOCUS       DATCUN010000032         3458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_170358, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000032 DATCUN010000000
VERSION     DATCUN010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170358"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VIO83_01175"
     CDS             complement(<1..642)
                     /locus_tag="VIO83_01175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8329929.1"
                     /translation="MPTTARLTLVLLSVFAVAAGAAPPAAAERRPYRSSQQILEASPA
                     SHWRMLDPDRTLYLELASGRVVIELAPQFAPEHVANITTLAREHFWDGTSIYRAQDNF
                     VVQFGDADGEDPAKAKPLGTARTHLPAEFHRSAKGLDFQKIPDSDGWAPQVGFVDGFP
                     AARDGAHGKAWLAHCYGTLGAGRNNADDSSIGAELYVTIGQSPRQLDDNITVVG"
     gene            complement(994..3015)
                     /locus_tag="VIO83_01180"
     CDS             complement(994..3015)
                     /locus_tag="VIO83_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HEY8329930.1"
                     /translation="MLRLLTSVFLLCSSLAALAAPLHVESPGKALSVEIDTDPDGRPS
                     YRVLRDGKPVIASSRLGFMLADAAKLERNLVLGNPRTRSFDETWEQPWGERRLIRNHY
                     NQLRVTLTEKVAPGRSLDVVFRVYDDGVGFRYEFPDQPQLKEVKISEELTEFNIAGEA
                     TAWWNQAFGSNRAEYLYQRTPIEQVGDAQTPITLRTIDGLHLSIHEAALVDYSGMTLT
                     RSEGRRLKAALTPGSGTFAKVERSAPFNTPWRTIQIGQRAGDLVESSLILNLNAPNTL
                     GDVSWVKPMKYVGVWWAMHLGKKTWGSGPDHGATTANVMRHIDFAARHHLGGVLVEGW
                     NIGWDGDWFGNGQDFDFSKPYPDYDLTRLAAYARSKGIRLIGHNETSADAAHYEAQME
                     AAFGLYQKLGIDAIKTGYVAAAGQARVIGRDGKVHYGWHEGQDMVNHAQRVVDAAARH
                     HIAVDAHEPVKDTGLRRTYPNFLTREGSRGMEYNAWGEPGNPPEHEVNLVFTRLLAGP
                     MDFTPGIFRMQTRPGARVSTTLAKQLALYVVLYSPLQMAADLLDNYEANPEPFKFIED
                     VAVDWEQTRVLNGEVGDYVTIVRKQRGAEDWFLGSITDEQPRTLQARLDFLTPGRRYT
                     AQIYRDADDAHWAKNPEAVKIQSRQVTSADTLTLKLAPGGGQAIRFVAH"
     gene            complement(3062..>3458)
                     /locus_tag="VIO83_01185"
     CDS             complement(3062..>3458)
                     /locus_tag="VIO83_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329931.1"
                     /translation="ALALVWIGARRRRVVAAAALLFWIVCTLAGALMLFIWFGTSHIA
                     GWANRNLLLFDPLCVLLLPAAVRALRGRALKAWSRWLLRGVAALALLAWMLHWFPFNV
                     QANMAWVALLVPVHLALAYVFTVQGSLSR"
BASE COUNT          541 a         1127 c         1197 g          593 t
ORIGIN      
        1 accgaccacg gtgatgttgt cgtccagctg gcgcggcgac tggccgatgg tcacgtacag
       61 ctcggcgccg atgctgctgt catcggcgtt gttgcggccc gcgcccagcg tgccgtagca
      121 gtgagccagc caggccttgc catgggcgcc atcgcgcgcg gccgggaagc cgtcgacgaa
      181 gccgacctgc ggggcccagc cgtcgctgtc ggggatcttc tggaaatcca gtcccttcgc
      241 gctgcgatga aactcggccg gcaggtgggt cctggccgtg cccagcggct tggccttggc
      301 cgggtcctcg ccatcggcat cgccaaactg gaccacgaag ttgtcctgcg cgcggtagat
      361 gctggtgccg tcccagaaat gctcgcgggc gagggtcgtg atgttggcca cgtgctcggg
      421 cgcgaactgc ggcgccagtt cgatcaccac ccggccgctg gccaattcca ggtacagcgt
      481 gcgatccggg tcgagcatgc gccagtggct ggccggcgag gcctccagga tctgctggct
      541 gctgcggtag ggccgccttt cggcggcggc cggcggggca gcgccggcag cgaccgcgaa
      601 cacggacagc aggacaaggg tcaggcgcgc ggtcgtcggc atgcggggct cgggatcaag
      661 gagcgagcga tcgattctga ctgcaggttt ccccgggcgc cagcagcatg gcttgcggcg
      721 gcgcgtgcca agttagttgt cgcctcagtc atgcagccgg ggactgtttt tctgccccct
      781 cccttgcgcg ttttttgcga aggggagggc cggggagggg ttgcttgtaa gcgcttgccc
      841 gaagcaacgg caacagcacc cctcccaacc ctcccctgct gcgcaaggga gggctcaaca
      901 gcacgcttcc caacttcccc tgctgcgcaa ggcagggcgc aacggcgatg gctgtcttgc
      961 aggctgggtc agggccggcg cggtgttcgc cgtttaatgc gcgacgaagc ggatcgcctg
     1021 gccgccgccg ggggccaact tcagggtcag cgtgtcggca ctggtgacct ggcgcgactg
     1081 gatcttcacc gcttccggat tcttggccca gtgtgcatcg tcggcatcgc gatagatctg
     1141 cgcggtgtag cgcctgccgg gcgtcaggaa atccagcctg gcctgcagcg tgcgtggctg
     1201 ctcgtcggtg atcgaaccga ggaaccagtc ttcggcgcca cgctgcttgc gcacgatggt
     1261 cacgtagtca ccgacctcgc cgttgagcac gcgggtttgc tcccagtcca cggccacatc
     1321 ctcgatgaac ttgaacggct cgggattggc ctcgtagttg tccagcaggt cggcggccat
     1381 ctgcagcggg ctgtagagga ccacgtacag cgccagctgc ttggccaggg tggtcgatac
     1441 gcgcgcgccc ggcctggtct gcatcctgaa gatgcccggg gtgaagtcca tcggcccggc
     1501 cagcagacgg gtgaacacca ggttgacctc gtgctcgggc ggattgcctg gctcgcccca
     1561 ggcgttgtat tccatgccgc gcgaaccttc gcgggtgagg aagttgggat aggtacggcg
     1621 caggccggtg tccttgaccg gttcatgcgc atccacggcg atgtggtgcc gggccgctgc
     1681 atcgaccacg cgttgggcgt ggttgaccat gtcctggcct tcgtgccagc cgtagtggac
     1741 cttgccgtcg cggccgatca cccgggcctg gccggcagcg gcgacatacc cggtcttgat
     1801 cgcgtcgatg ccgagtttct ggtacagccc gaaggcggct tccatctgcg cctcgtaatg
     1861 tgccgcgtcg gccgaggtct cgttgtggcc gatcaggcgg atgcccttgg acctggcgta
     1921 ggcagccagc ctggtcaggt cgtagtccgg gtacggtttg ctgaaatcga agtcctggcc
     1981 gttgccgaac cagtcgccat cccagccgat gttccagcct tccaccagca ccccgcccag
     2041 gtgatgcctg gcggcgaagt cgatgtggcg catcacgttg gcggtggtcg caccgtggtc
     2101 gggaccggag ccccaggtct tcttgcccag gtgcattgcc caccagaccc cgacgtactt
     2161 catcggcttg acccaggaca catcgcccag tgtgttgggc gcgttgaggt tgaggatcag
     2221 gctggactcg accaggtcgc cggcgcgctg gccgatctgg atcgtgcgcc acggcgtgtt
     2281 gaacggcgca ctgcgctcga ccttggcgaa ggtgccgctg cccggcgtca gcgcggcctt
     2341 gagccggcga ccttcgctgc gggtgagggt catgcccgaa taatccacca gcgccgcttc
     2401 gtggatggac aggtgcaggc catcgatggt gcgcagggtg atcggggtct gtgcatctcc
     2461 gacttgttcg atcggcgtgc gctggtagag gtactccgcg cggttcgagc cgaaggcttg
     2521 gttccaccag gcggtggcct cgccggcgat gttgaactcg gtcagctcct cgctgatctt
     2581 gacctctttg agctgcggct ggtcggggaa ttcgtagcgg aagccgacgc catcgtcgta
     2641 cacgcggaac accacgtcca ggctgcgccc gggcgcgacc ttctcggtca gggtgacgcg
     2701 cagctggttg tagtgattgc ggatcaggcg gcgctcgccc cagggctgtt cccaggtctc
     2761 atcgaagctg cgcgtgcgcg ggttgcccag gaccaggttg cgttcgagct tggccgcatc
     2821 ggccagcatg aaacccagcc gcgaggacgc gatgaccggc ttgccgtcgc gcagcacccg
     2881 atagcttggg cggccatcgg gatcggtgtc gatctccact gacagggcct tgccggggga
     2941 ctcgacatgc agcggcgcag ccagcgccgc cagcgacgaa cagagcagga agacagaagt
     3001 gagaagacgc agcatgcggg gggctccagc tggaagtgac cgacatgctc gcccgcgatg
     3061 atcagcgcga caagctgccc tgcacggtga atacgtatgc aagcgccaga tgcaccggca
     3121 ccagcagcgc gacccaggcc atgttggcct gcacgttgaa cgggaaccag tgcagcatcc
     3181 acgccagcag cgccagcgcc gccacgccgc gcagcagcca gcgtgaccag gccttcagcg
     3241 cccgtccgcg cagcgcccgc accgcggccg gcagcagcag cacgcacagc ggatcgaaca
     3301 gcaacaggtt gcggttagcc cagccggcga tgtggctggt gccgaaccag atgaacagca
     3361 tcagcgcgcc agccagtgtg cacacgatcc agaacagcag cgccgcggct gcgaccaccc
     3421 gccgccggcg cgcaccgatc cacaccaggg ccagggcg
//
LOCUS       DATCUN010000033         8462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_207845, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000033 DATCUN010000000
VERSION     DATCUN010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207845"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VIO83_01190"
     CDS             complement(<1..405)
                     /locus_tag="VIO83_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY8329932.1"
                     /translation="MDTQAIRAQMPTLVVGHVPSNVRSFKFNIFDGQPKVSTLGFHID
                     PKPFEGKVIATTDEAIVVKTGRAEFAVLDRTLVTEVPDEGAKVQVEPYVRRRFDGQRA
                     DTPEEQTEFTADGQPYTVKRLVLGSAPAKLPIP"
     gene            complement(696..1412)
                     /locus_tag="VIO83_01195"
     CDS             complement(696..1412)
                     /locus_tag="VIO83_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8329933.1"
                     /translation="MSHSKKPFVRGYDGLSVQRLLAISYDDDCPLSYLPLHVSQSHLP
                     DSQVERHACVFCDDFALITEGQNVPPELGAQCPSHGIARNLVYAVMAEEAGKPLHVGD
                     TYSEEAAREVVRRLRFETGFYGRAWEISSAHITEEAGRFLAELADIATPSGFLFVAFR
                     IPYSPAVGMKLIATPWTDANLQHVKGITAEELRQEHRAKGVPESLVEVLHLAALADVR
                     MLVFDADAPVLDGLTLYDDE"
     gene            complement(1663..2055)
                     /locus_tag="VIO83_01200"
     CDS             complement(1663..2055)
                     /locus_tag="VIO83_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329934.1"
                     /translation="MDLSLPEDVLDKMALEQAHFEAAPQAFFEAWKRGAQIAGHEWFG
                     DGTREGLQRATTKWDLRPNMLMLNDALGVLSSGQRMFLSAMVSFYNSREGGAMLKRCG
                     FEGLSDFGGLDLERRQVIADLTLHYNGW"
     gene            complement(2081..2317)
                     /locus_tag="VIO83_01205"
     CDS             complement(2081..2317)
                     /locus_tag="VIO83_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329935.1"
                     /translation="MSEPSASSSATAVRSGALALAWTGKRLPLQVLRSAAGHYIGTQG
                     DEGPVSRESVEYFPTRMAAQRALDTDAWTQRAHP"
     gene            complement(3097..5112)
                     /locus_tag="VIO83_01210"
     CDS             complement(3097..5112)
                     /locus_tag="VIO83_01210"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016852000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEY8329936.1"
                     /translation="MRVFLCEKPSQGKDIARVLGAVQRGNGCYSGAGVVVTWCIGHLV
                     EAVPPEGYGAQYKRWAIEQLPILPERWRLEPKAATAAQFEVVQQLVAKAGELVIATDA
                     DREGEMIAREIIDLCDYRGPIQRLWLSALNDASIRKALGALKPSAQTLPLYFSALARS
                     RADWLIGMNLSRLFTLLGRQAGYPGVLSVGRVQTPTLKLVVDRDREIARFVSAPYWTV
                     DVLLSHAGQSFTASWIPPEGSTDAAGRCLQQPVAQHAADRIRAACDAQVVSVDTERVR
                     EAPPLPFDLGTLQEVCSRQLGLDVQETLDIAQALYETHKATTYPRSDSGYLPESMLAE
                     VPAVVDALLATDPSLRPLIGQLDRNQRSRAWNDGKVTAHHGIIPTLEPAKLPAMSEKE
                     LAVYRLIRGRYLAQFLPHHEFDRTVAQLACDGQPLVAVGKQIAVSGWRQVLATPEPDD
                     ADGEDAQRSQVLPPLASGSCCGVEHVELKVLKTLPPKPYTQGELVKAMKGVAKLVTDP
                     RLKQKLKDTTGIGTEATRASTIKGLLDRGYLVKKGRAVRASDAAFTLVDAVPSAIADP
                     GTTAVWEQALDMIEAGQMTPDTFIAKQSAWVAQLVRQYRGATLSIGLPPAPACPQCGA
                     PVRQRTGKGGAFWSCSRYPDCKGTLPIESPTGRRSAPRKRRAASKAS"
     gene            complement(5391..5840)
                     /locus_tag="VIO83_01215"
     CDS             complement(5391..5840)
                     /locus_tag="VIO83_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY8329937.1"
                     /translation="MSTHFVGEGNIGSAPDYREFPNGNDEPRRLLRLNVYFDNPIPKK
                     DGEFEDRGGFWAPVELWHRDADHWKDLYQKGMRVLVEGRTVKDEWEDADENERVTFKV
                     EARRVGILPYRLQAVTLSPRTLEPEAAVPLPKETKATKAPKESKPKG"
     gene            complement(5837..6388)
                     /locus_tag="VIO83_01220"
     CDS             complement(5837..6388)
                     /locus_tag="VIO83_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3158 family protein"
                     /protein_id="HEY8329938.1"
                     /translation="MMASPPTGQPGRFAPLEQADFQRLEHAGYLKGLLQPFKGKGSLE
                     TWANQCTALRDELIALAQRRVLKQARAYPFTLLDVQLAQQATGAGTTFLRWRNHDRSS
                     MGVALWESLLDRPATPASLIDDLYAMELQRIALNMQISLTHSIARQALECASKAAQAE
                     AVYLRRVHGRTATVSFTTPKESS"
     gene            complement(6385..7158)
                     /locus_tag="VIO83_01225"
     CDS             complement(6385..7158)
                     /locus_tag="VIO83_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03761 family integrating conjugative element
                     protein"
                     /protein_id="HEY8329939.1"
                     /translation="MANEPLQLNLGSLRSAMSLTLHTHHASRIWHGRAPAEGRPGIIG
                     LNGYISVMNKMKRGAEQDDPYSDWWMLRIEDKLTQTKASLQALREQVDQALADVPPAL
                     TLGENMNVQPVKLPLFVNAQLGFMAVYLLADYDDLARKLILAHHTALIDRSTLERWLN
                     DGAHALRSLFSLAQQYRYSGTRRDDFAAKNAAARAALEKFGELPQDVLEGTRRSRFAP
                     PVARRTGKPGTPAAAPAADAAAPASTDRAADAAFEDASP"
     gene            complement(7357..8211)
                     /locus_tag="VIO83_01230"
     CDS             complement(7357..8211)
                     /locus_tag="VIO83_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OST-HTH/LOTUS domain-containing protein"
                     /protein_id="HEY8329940.1"
                     /translation="MSELADGDLQDHQREVQRLLGRCMLRLQQYERLIKAIVAYHDLT
                     GPAHALESIRAARIADAAGKTLGALVGQFLGSYVVTDGADTSADALGKAPAEVTSFSM
                     RMHLSLSAEDYAQTQDGLRDLVSLRNNLVHHFIDQHDLWSSDGCREAHEALAAAYARI
                     DQHFERLRGWAEHMDQARRLAAEFVQSDAFRDLVIHGITPDGSVDWPAAGIVRALREA
                     SVELAVEGWAPVASAGRWITQRYPDQQPARYGCASWRQVLHESRLFELRYRDVDGRRA
                     AWYRARTH"
     gene            complement(8208..>8462)
                     /locus_tag="VIO83_01235"
     CDS             complement(8208..>8462)
                     /locus_tag="VIO83_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003294157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY8329941.1"
                     /translation="TRDRWGTEHADRYITGLFEAFDGIQTHRTSSRPIPADFGIEGYF
                     FRYERHFVYWRRLSNGDIGIVTILHERMHQIDRFRDDFGS"
BASE COUNT         1407 a         2700 c         2925 g         1430 t
ORIGIN      
        1 gggaatcggc agcttcgccg gtgcggaccc gagcaccaac cgcttcaccg tgtagggctg
       61 gccgtcggcg gtgaactcgg tctgctcctc gggcgtgtcc gcccgctgac cgtcgaagcg
      121 gcgtctgaca tagggttcga cctgcacctt ggcgccctcg tcgggcactt cggtcacgag
      181 cgtgcgatcg agcaccgcga actcggcccg tcccgtcttg acgacgatgg cctcgtcggt
      241 ggtggcgatg accttgccct cgaaaggctt cgggtcgatg tgaaagccca gcgtcgaaac
      301 cttgggctgg ccgtcgaaga tgttgaattt gaacgaacgg acgttggaag gcacatgacc
      361 gacaacgagc gtcggcatct gtgcgcgaat ggcttgggta tccatgggga gactccttgt
      421 ggcaaccaag ggactcccgc ccgcaaggga ggtctgtccc ttgagggtga ggtggatgga
      481 cgcgggtgcg cccggagaac gaagcaacca gcacggcgaa ccgcgccgca gtcttgaagg
      541 tctcgactgc ctgggcatgc tgccggcaac gccagcgaac atgcggccag cgtgcggcac
      601 atggcggcga ccgtccgttg ggaatcggca atgcgccggc accgcgttcc gcgaaaaaga
      661 agagccccga cgtgggggct cgaatgggtt gcgggttact cgtcgtcgta gagcgtcagc
      721 ccgtccagca ctggcgcgtc ggcatcgaac accaacatgc gaacgtcggc gagcgcagcc
      781 aggtgcaaca cttccacgag ggactccggc acgcccttgg cccggtgctc ctgccgcagc
      841 tcttcggcgg tgatgccctt gacatgctgc aggttcgcat ccgtccaggg cgtggcgatc
      901 agcttcatgc cgaccgccgg gctgtaggga atgcggaagg ccacgaacag aaaaccgctc
      961 ggcgtggcga tgtccgccag ttcggcgagg aagcgaccgg cctcctcggt gatgtgcgcc
     1021 gaactgattt cccaggcacg gccgtagaac ccggtctcga agcgcaggcg gcgtaccacc
     1081 tcgcgcgcgg cttcctcgga gtaggtgtcg ccgacgtgca gcggcttgcc ggcttcttcg
     1141 gccatgacgg cgtagacgag gtttcgggcg ataccgtggc tggggcactg cgcgcccagc
     1201 tcgggcggca cgttctggcc ctcggtgatc agcgcgaagt cgtcgcagaa gacgcaggca
     1261 tggcgctcga cctggctgtc cggcaggtgc gactgcgaga cgtgcagcgg cagatagctc
     1321 agcgggcagt cgtcgtcgta ggaaatcgcc agcagccgct gcacggacag gccatcgtag
     1381 ccgcggacga agggtttttt ggaatgagac atgggattcc tccagcagag gatggccgag
     1441 gcaatcccct gccggggatt gaaccccagc gagtggatga agtggcagcc ggtcagccgg
     1501 ccgcggcgtc tggccgccct ggtggcgggc ggtgcaggtc agtggaagat gctgattcgg
     1561 gacatggccg ctcctgcgaa aggaaggagg gacatggccc cgaacgggga cgcatgtccc
     1621 tcgtggggtg ggatgaaaag acggcgggtt gccaggcgcg gctcaccagc cgttgtagtg
     1681 cagcgtgagg tcggcgatga cctggcgccg ttccagatcg aggccgccga agtcggacag
     1741 cccctcgaag ccgcaacgct tgagcatcgc gccgccctcg cgggagttgt agaagctcac
     1801 catcgcggac aggaacatgc gttgaccgct gctcagcaca cccagggcgt cgttgagcat
     1861 cagcatgttg ggccgcaggt cccacttggt ggtggcacgc tgcagacctt cgcgtgtgcc
     1921 gtcgccgaac cactcgtggc cggcgatctg tgcgccgcgc ttccaggcct cgaagaaggc
     1981 ctgcggtgca gcctcgaagt gcgcctgttc cagcgccatc ttatcgagca cgtcttcggg
     2041 taaagacaga tccatgagag aactccttga agggtgggaa tcaggggtga gcgcgctgtg
     2101 tccaggcatc ggtatccaga gcgcgctgtg ctgccatgcg agtcgggaaa tactcgacgg
     2161 actcccgcga caccgggcct tcgtcgcctt gtgtgccgat gtagtggccg gccgcgctgc
     2221 gcagcacctg tagcggtaga cgcttgccgg tccaggccag cgccagtgca ccactgcgca
     2281 cagcggttgc agaggaagat gcggaaggtt cagacatgcc gtgctccttg gtgggtcgga
     2341 gagcgcgcat cccgcagggg atggggttcc cctcgggtga gtgagaaaag ggcatcgtcg
     2401 ccaggccggc gagcgtccgc ggtgggcgat gcgaagcgga ctggatcggt caacgcggcg
     2461 aaccgcgctg cagtcttgaa gaccgggact gccaggggca tgccgctgac gactgtcagc
     2521 aatgcatggc gtgaggatgg gcaccaggca tgactgccgt cgcagggaaa accgactcgc
     2581 ggacggcccg ctttaggcca agtccgcgtc acgggacaag gcgaggacca gggcgccgaa
     2641 ggccacgcgg ccttcggctg gagaggaagg aaaaccacct gtgggcggcg tcagcgtagg
     2701 ccgtcgtcaa agccatccgc tgcgtcagca atcgcacccg gcccgtttcg tggctggggc
     2761 cggaaatctc tggcgtgcat ccacacacaa agggagcccg ttgttccgcc ggcgggcacc
     2821 ggcacggaat accctcggcc caaggggcct cctcacggct cgcgcgccgg caagatgtca
     2881 ggaaggccct cgtggccgag gaaaggcaca ccgatcaagg gaatggcggc ggacgcgatt
     2941 cgtggagcaa tgaccttccc gccccgtcgc ccggtggtgg gcagacgggg cgcgcacacc
     3001 gttgcagaag gagacgcgcg ctttggagtc ccacgcggtg cgggacgttt gcctcgccgc
     3061 cagtgaagtg gccggcgcgg caggggcaag cgaggatcag gacgccttgg aggcagcgcg
     3121 ccgcttgcgc ggtgcgctgc gccggcccgt gggagactcg atcggcaacg tgcccttgca
     3181 gtctgggtag cgcgagcaag accagaatgc gccgcccttt ccggtacgct ggcgcactgg
     3241 tgcaccgcac tgcgggcagg ccggcgccgg cggcagcccg atggaaagcg tcgcgccccg
     3301 atactggcgc acgagctggg ccacccaggc ggattgcttg gcgatgaagg tgtccggcgt
     3361 catctggccg gcctcgatca tgtcgagcgc ctgctcccac accgccgtgg tgccggggtc
     3421 ggcgatggcc gagggcaccg cgtcgacgag cgtgaatgcc gcgtcggaag cacggacggc
     3481 gcggcccttc ttgaccaggt agccgcgatc gagcaggccc ttgatcgtgc tggcgcgcgt
     3541 cgcctcggtg ccgatacccg tggtgtcctt cagcttctgc ttcaggcgcg ggtcagtcac
     3601 gagcttggcg acgcccttca tggccttgac cagctcgccc tgggtgtagg gcttcggcgg
     3661 cagcgttttc aacaccttca gctcgacatg ctcgacccca cagcacgatc cgctcgccag
     3721 cggcggcagc acctggctgc gctgcgcatc ctcgccatcg gcatcgtccg gctccggtgt
     3781 cgccagtacc tggcgccagc cgctcacggc gatctgcttg cccaccgcca cgagcggctg
     3841 cccgtcgcaa gcgagctgcg ccaccgtgcg gtcgaattca tggtgtggaa gaaactgcgc
     3901 gagatagcgc ccgcggatga gccggtagac cgccagttct ttctccgaca tggccgggag
     3961 cttggccggc tccagcgtgg ggatgatgcc gtggtgcgcc gtcaccttgc cgtcgttcca
     4021 ggcgcgcgaa cgctggttgc ggtcgagctg gccgatcaag ggccgcaggc ttgggtcggt
     4081 ggcgagcagt gcatcgacca ctgctggcac ttccgcgagc atgctttcgg gcagatagcc
     4141 cgagtcactg cgcggatatg tcgtcgcctt gtgcgtctcg tacagcgcct gcgcgatgtc
     4201 caacgtctcc tgcacatcga ggccaagctg acgcgaacac acttcctgca aggtgcccaa
     4261 gtcgaacggc agcggcggtg cctcgcgcac acgctcggtg tccaccgaca cgacctgcgc
     4321 atcgcatgcc gcgcgaatgc gatccgcggc atgctgcgcc accggctgct gaaggcagcg
     4381 acccgctgca tcggtgctgc cttcgggcgg tatccagctc gcagtgaagg actggccggc
     4441 atgggacagc agtacgtcca cggtccaata cggcgcagag acgaagcgcg cgatctcgcg
     4501 gtcccgatcc acgaccagct tcagcgtggg cgtttgcacg cggcccaccg acagcacgcc
     4561 gggatagccc gcctggcgcc cgagcagggt gaacaagcgg ctcaggttca ttccgatcag
     4621 ccagtcggcg cgcgaccggg cgagcgcaga gaaatacagc ggcagggtct gggcggacgg
     4681 cttgagcgca cccagagcct tgcggatcga cgcatcgttg agcgccgaca gccacaggcg
     4741 ctgaatcggc ccgcggtagt cgcacaggtc gatgatctcg cgggcgatca tctcgccctc
     4801 gcggtcggcg tcggtcgcga tcaccagctc gcccgccttg gctacaagct gctgcacgac
     4861 ctcgaattgc gccgcggtcg ccgccttggg ctcgagacgc cagcgctcag gaagaatggg
     4921 cagttgctcg atggcccagc gcttgtattg cgcgccgtag ccttcgggcg gaaccgcctc
     4981 caccagatga ccgatgcacc aggtcacgac gacacccgcg ccgctgtagc agccgttgcc
     5041 gcgttgaacg gcacccagca cacgggcgat gtccttgccc tgggagggtt tctcgcacag
     5101 gaacacacgc atgaagcctc cttggaaatg tgcgactcgt cggttgggcg ccagcttggc
     5161 ggggccggga gatgccggca gcaaggaagc cgactggtgc agacaccgct ttgtgatcgg
     5221 caggcgatgc gttgccgcag cggcatgcgc ggcagtgcaa cggctggcgc aacgggggcg
     5281 tcgtttcggg agggcggctg gaattcagtg gacgaccctg gcgctatccc ctggggatag
     5341 ttggtgcatg acgggcgtct gacggttgcc acagccgccc ctgtagggaa tcagcccttg
     5401 ggcttgcttt cctttggcgc cttcgtcgcc ttggtttcct tcggcagcgg tactgccgcc
     5461 tcgggttcca gcgtccgggg gctgagggtc acggcctgga ggcggtacgg caggatgccc
     5521 acgcgccggg cctcgacctt gaacgtgacg cgctcgttct cgtcagcgtc ctcccactcg
     5581 tccttcacgg tccggccttc caccagcacg cgcatgcctt tctggtacag gtccttccag
     5641 tggtcggcgt ctcgatgcca cagctccacc ggcgcccaaa agccgccgcg gtcctcgaac
     5701 tcgccgtcct tcttcgggat cgggttgtcg aagtagacgt tcaggcgcag caaacggcgc
     5761 ggctcgtcat tgccgttggg gaattcgcga tagtcaggcg ctgaaccgat attgccctcg
     5821 ccgacgaagt gcgtgctcat gatgactcct tgggggtggt aaatgaaacg gtcgcggtac
     5881 gtccgtggac acgccgcagg taaaccgctt cggcctgggc ggccttgctg gcgcattcca
     5941 gggcttggcg agcgatgctg tgggtcaggc tgatctgcat gttcagcgcg atgcgctgca
     6001 actccatcgc gtacaggtcg tcgatcagcg aggccggcgt cgccgggcgg tcgagcagcg
     6061 actcccacag cgccacgccc atggaagaac ggtcgtggtt gcgccagcgc aggaaggtcg
     6121 tccctgcgcc agtggcctgc tgggccagtt gcacgtcgag cagggtgaag ggataggccc
     6181 gcgcctgctt cagcacacgc cgctgcgcca gcgcgatcaa ctcgtctcgc agcgccgtgc
     6241 attggttggc ccaggtctcc agacttccct tacctttaaa gggctgtaaa aggcctttta
     6301 ggtagccggc atgttccaga cgttggaagt ctgcctgttc cagcggcgcg aagcgtcccg
     6361 gttggccggt cgggggcgat gccatcatgg gctggcatcc tcaaacgcgg catcggctgc
     6421 gcgatccgtg gaagctggtg ccgccgcatc agcggccggc gcagcagccg gtgtgccagg
     6481 cttgcctgtg cggcgcgcga cgggtggcgc aaagcgcgaa cggcgcgtgc cttccagcac
     6541 gtcctgcggc agctctccaa acttctccag cgccgcacgc gccgcagcat tctttgccgc
     6601 gaaatcgtcg cgcctcgtgc cggagtagcg gtactgctgg gccagcgaga acaggctgcg
     6661 cagcgcgtgc gccccatcgt tgagccagcg ctccaaggtg ctgcggtcga tcagcgcggt
     6721 gtggtgggcg aggatcagct tgcgcgccag gtcgtcgtag tcggccagca ggtacaccgc
     6781 catgaaaccg agctgcgcat tgacgaacag cggcagcttg accggctgca cgttcatgtt
     6841 ctcgcccagc gtcagcgccg gaggcacatc ggccagcgcc tggtccacct gctcgcgcag
     6901 ggcctgcaag ctggccttgg tctgcgtcag cttgtcctcg atgcgcaaca tccaccaatc
     6961 cgagtacggg tcgtcctgct cggcgccgcg tttcatcttg ttcatcacgc tgatgtagcc
     7021 gttgaggccg atgattccgg gccggccctc ggccggcgct cggccgtgcc agatgcgcga
     7081 agcgtgatgc gtatgcagcg tcagcgacat cgcgctgcgc agcgatccga gattcagttg
     7141 caggggttcg ttggccatgg gtgacgactc gcggtaaggg agcgagtcgc cagcattggc
     7201 atcggccaga aggccgtcag tcaacaaacc gcagcccata tgtgcccggt ttgttcgctg
     7261 ccggaaggct ggctgtaccg gctatcccct ggggatagcc tgtgagatga cagcaggacg
     7321 gggaagccgc cgatgcggca ccatgacctc gactggtcag tgggtgcgag ccctgtacca
     7381 ggcggcacgc cgcccgtcca cgtcacggta gcgcagttcg aagaggcgcg actcgtgcag
     7441 cacctgccgc caactggcgc agccgtacct ggcaggttgc tggtcgggat accgttgcgt
     7501 gatccatcgt cccgcgctgg caacgggtgc ccagccttcc acggccagct cgaccgaggc
     7561 ctcgcgcagg gcgcggacga tgcctgctgc cggccaatcc accgagccat ccggggtgat
     7621 gccatggatc accaggtcac ggaaggcgtc ggactgcacg aactcggcag ccaggcgcct
     7681 ggcctggtcc atgtgttcgg cccagccccg gaggcgctcg aagtgctggt cgatgcgagc
     7741 gtaggcggca gccagcgcct cgtgcgcttc acgacatccg tccgagctcc acaggtcgtg
     7801 ctggtcgatg aagtggtgca ccagattatt gcgcagcgac accaaatccc tcaggccgtc
     7861 ctgggtctgg gcatagtctt ccgcggacag gctcaggtgc atccgcatgc tgaacgaggt
     7921 gacttctgcc ggggccttgc ccaaggcatc ggcggatgtg tcggctccat cggtgacgac
     7981 atacgatccc aggaactggc ccaccagggc gccgagcgtc ttgccagcgg catcggcaat
     8041 gcgcgccgcg cggatcgact ccagtgcgtg ggccggtccc gtgagatcgt ggtacgccac
     8101 gatggccttg atcaggcgct cgtactgctg gaggcggagc atgcagcggc cgagcaagcg
     8161 ctggacttca cgctggtggt cctgcagatc gccgtcggcc aattcgctca tgaaccgaaa
     8221 tcgtcccgga atcggtcgat ctggtgcatc cgctcgtgca ggatggtcac gatgccgatg
     8281 tcgccattgg agagccggcg ccagtagacg aagtgccgtt cgtagcggaa gaagtagcct
     8341 tcgatgccga aatcggcggg aatgggccta gacgatgtcc ggtgcgtctg gatgccgtcg
     8401 aaggcctcga acaggccggt gatgtagcgg tcggcgtgtt cggtgcccca gcgatcgcgc
     8461 gt
//
LOCUS       DATCUN010000034         5260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_189106, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000034 DATCUN010000000
VERSION     DATCUN010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189106"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..3148
                     /locus_tag="VIO83_01240"
     CDS             8..3148
                     /locus_tag="VIO83_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8329942.1"
                     /translation="MSRFFIDRPIFAWVIALVIMLAGGLSILKLPINQYPSIAPPAVG
                     IQVSYPGASAQTVQDTVVQVIEQQLNGIDGLRYISSESNSDGSMTITATFNQGVDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGIRVTKAVKNFLLVIGLVSEDGSMDKNDLADYIVA
                     NIQDPLSRTKGVGDFQVFGAQYAMRVWLDPAKLNNFALTPVDVKSAIQAQNVQISSGQ
                     LGGLPASPGQQLTATIIGKTRLQTPEQFREILLKVNADGSQVRLKDVARVELGGENYS
                     ISAEYNGLPATGIAVKLATGANALDTVAAVRETLDELEPFFPPGMKAVYPYDTTPVIS
                     ASIMGVVTTLFEAIGLVFLVMYLFLQNFRATLIPTLAVPVVLLGTFGILAAFGFSINT
                     LTMFAMILAIGLLVDDAIVVVENVERVMSEEGLSPLEATRKSMGQIQGALVGIALVLS
                     AVFVPMAFFPGSTGVIYQQFSITIVSAMVLSVLVALIFTPALCATLLKPIAKGEHHHK
                     GGFFGWFNRSFQRGVDGYERGVKAVLRRKAPYLLLYLVIVAIMAWMFTRIPTAFLPEE
                     DQGVLFSQVQTPAGASAERTYEVVKQMREYLLTEEKDVVKSVFTVTGFNFAGRGQSSG
                     MAFINLRPWSERSAPGQSVFELAQRAQMRFFGFRDAMVFAFAPPAVMELGNATGFNVF
                     LQDRAGVGHQVLTQARDQFLQLASQSPKLAAVRMNGLRDEPQYQLLIDDEKARAQGVS
                     LAEIDQTLSIAWGGSYVNDFIDRGRVKKVYLQGTAGARMNPEDLDKWFVRNAAGQMVP
                     FSAFASGQWIYGPPKLARYNGVPAMEIMGAAAPGYSSGEAMAEVEKLAEQLPPGVGLA
                     WTGLSYEERLAGGQAPALYALSLLVVFLCLAALYESWSIPFSVMLVVPLGIIGALLAT
                     YGRGLSNDVYFQIGLLTTIGLSARNAILIVEFAKSLHAQGMSLVEAAAQACRMRLRPI
                     VMTSLAFILGVVPLAISSGAGAGSKHAIGTGVIGGMLSALVLAIFWIPLFYVLVCSLT
                     ERKRRPGVQAEKESAQ"
     gene            3145..4539
                     /gene="adeC"
                     /locus_tag="VIO83_01245"
     CDS             3145..4539
                     /gene="adeC"
                     /locus_tag="VIO83_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AdeC/AdeK/OprM family multidrug efflux complex
                     outer membrane factor"
                     /protein_id="HEY8329943.1"
                     /translation="MKKSILSLSIVLALGGCSLIPDYQRPDAPVANAWPQGEAYAASA
                     TAAAALPEWRSFFRDPALQQLIGQALENNRDLRAAALNVEAYQALYRIQRAELFPQLD
                     ATGSGTRQRLPADLTRTGQAETNGEYRASLGVTDWEVDLFGRLRSLNQAALEQYFATE
                     QAQRATQISLVASVANAWLVLQADSAQLKLSQETLQTYEQSYNLTRRSFDVGVASALE
                     LQQSRTVVESARSSVAQFTRLLAQDRNALQVLLGGPLPADLPEQQGLEGALLAELPVG
                     LPSELLQRRPDILQAEHALQAANANIGAARAAFFPSISLTARAGTTSAELSGLFKGGS
                     GTWLFQPQINLPIFTAGSLKANLDYSRLQKDVQVAQYERAIQVAFSEVADGLAARGTY
                     QQQLQAQRDLVEASAEYYRLAETRYRTGVDSRLTLLDAQRQLFSARQQLIDVRLAQLG
                     SEIDLFKALGGGVE"
     gene            complement(4951..>5260)
                     /locus_tag="VIO83_01250"
     CDS             complement(4951..>5260)
                     /locus_tag="VIO83_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019360412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8329944.1"
                     /translation="PSLPPAWLHKPFADWCRDLALLKQLPAPGARDWSALEAAGWQRL
                     AEVRNLELLRGLFRRPLEVWLLLDRALYLEEQGYRVQLGQFCDYQLTPRNLLLLAERD
                     "
BASE COUNT          834 a         1789 c         1790 g          847 t
ORIGIN      
        1 ttcgctgatg tctagattct ttatcgaccg gccgatcttc gcctgggtga tcgccctggt
       61 gatcatgctg gccggcgggc tgtcgatcct caagctgccg atcaaccagt acccgagcat
      121 cgcgccgccg gcggtgggca tccaggtcag ctacccgggc gcttcggcgc agacggtgca
      181 ggacaccgtg gtgcaggtga tcgagcagca gctcaacggt atcgacgggc tgcgctacat
      241 ctcctcggag agcaactccg acggcagcat gaccatcacc gccaccttca accagggcgt
      301 cgacccggac atcgcccagg tgcaggtgca gaacaagctg cagctggcca ccccgttgct
      361 gccgcaggaa gtgcagcagc agggcatccg cgtgaccaag gcggtgaaga acttcctgct
      421 ggtgatcggc ctggtgtccg aagacggcag catggacaag aacgacctgg ccgactacat
      481 cgtcgccaac atccaggacc cgctgtcgcg gaccaagggc gtcggcgact tccaggtgtt
      541 cggcgcgcag tacgccatgc gcgtctggct cgatccggcc aagctgaaca acttcgcgct
      601 gaccccggtg gacgtgaaga gcgcgatcca ggcgcagaac gtgcagatct cctccggcca
      661 gctcggcggc ctgccggcct cgccgggcca gcagctgacc gccaccatca tcggcaagac
      721 ccgcctgcag actcccgagc agttccgcga gatcctgctc aaggtcaacg ccgacggctc
      781 gcaggtgcgc ctcaaggacg tcgcccgggt cgagttgggc ggcgagaact acagcatcag
      841 cgccgagtac aacggcctgc cggccaccgg catcgcggtc aagctggcca ccggcgccaa
      901 cgcgctagat accgtggcgg cggtgcgcga gaccctcgac gagctggagc cgttcttccc
      961 gccgggaatg aaggcggtct atccgtacga caccacgccg gtgatctcgg cgtcgatcat
     1021 gggggtggtg accaccctgt tcgaggccat cggcctggtg ttcctggtga tgtacctgtt
     1081 cctgcagaac ttccgcgcca ccctgatccc gaccctggcg gtgccggtgg tgctgctcgg
     1141 caccttcggc atcctcgcgg cgttcggttt cagtatcaac accctgacca tgttcgccat
     1201 gatcctggcc atcggcctgt tggtcgacga cgccatcgtg gtggtggaga acgtcgagcg
     1261 ggtgatgagc gaggagggcc tgtcgccgct ggaggccacg cgcaagtcga tggggcagat
     1321 ccagggcgcg ctggtcggca tcgccctggt gctgtcggcg gtgttcgtac ctatggcgtt
     1381 cttccccggc tccaccgggg tgatctacca gcagttctcg atcaccatcg tctcggccat
     1441 ggtcctctcg gtgctggtgg cgctgatctt caccccggcg ctgtgcgcca ccctgctcaa
     1501 gcccatcgcc aagggtgagc atcaccacaa gggcggcttc ttcggctggt tcaaccgcag
     1561 cttccagcgc ggggtcgacg gttacgagcg cggcgtcaag gcggtgctca ggcgcaaggc
     1621 gccctacctg ctgctgtacc tggtgatcgt cgcgatcatg gcctggatgt tcacccgcat
     1681 tcccaccgct ttcctcccgg aggaggacca gggcgtgctg ttcagtcagg tgcagacccc
     1741 ggccggcgcc agcgccgagc gcacctacga ggtggtcaag cagatgcgcg agtacctgct
     1801 gaccgaggaa aaggacgtgg tcaagtcggt gttcaccgtt accggcttca acttcgccgg
     1861 tcgcgggcag agctcgggca tggccttcat caacctgcgg ccgtggagcg agcgcagcgc
     1921 gccggggcag agcgtgttcg agctggcgca gcgcgcgcag atgcgcttct ttggcttccg
     1981 cgacgccatg gtgttcgcct tcgccccgcc ggcggtgatg gagctgggca acgccaccgg
     2041 cttcaacgtg ttcctccagg accgcgccgg tgtcggccat caggtgctga cccaggcccg
     2101 cgaccagttc ctccagctgg ccagccagag cccgaaactg gcggcggtgc ggatgaacgg
     2161 tctgcgcgac gagccgcagt accagctgct gatcgacgac gagaaggcgc gcgcccaggg
     2221 cgtgtcgctg gcggagatcg accagaccct gtcgatcgcc tggggcggca gctacgtcaa
     2281 cgacttcatc gaccgcggcc gggtgaagaa ggtctacctg cagggcacgg ccggggcgcg
     2341 gatgaacccc gaggatctgg acaagtggtt cgtgcgtaat gccgccgggc agatggtgcc
     2401 gttcagcgcc ttcgccagcg gccagtggat ctacggcccg cccaagctgg cgcgctacaa
     2461 cggcgtgccg gcgatggaga tcatgggcgc cgcggcgccg ggctacagct cgggcgaggc
     2521 gatggccgag gtcgagaagc tcgccgagca gctgccgccg ggcgtgggtc tggcctggac
     2581 cgggctgtcc tacgaggaac gcctggccgg cggccaggcg ccggcgctgt atgcgctgtc
     2641 gctgctggtg gtgttcctct gcctggcggc gctgtacgag agctggtcga ttccgttctc
     2701 ggtgatgctg gtggtgccgc tggggatcat cggcgcgctg ctggccacct acggtcgcgg
     2761 gctgtccaac gacgtgtact tccagatcgg cctcttgacc accatcggcc tgtcggcgcg
     2821 caacgcgatc ctcatcgtcg agttcgccaa gtcgctgcac gcgcagggca tgagcctggt
     2881 cgaggccgcg gcgcaggcct gccgcatgcg tctgcggccg atcgtgatga cctcgctggc
     2941 gttcatcctc ggcgtggtgc cgctggcgat ttccagcggc gccggcgccg gcagcaagca
     3001 cgccatcggc accggggtga tcggcggcat gctctccgcg ctggtactgg cgatcttctg
     3061 gatcccgctg ttctatgtac tggtctgctc gctgaccgag cgcaagcgcc ggccgggcgt
     3121 tcaggccgag aaggagtccg cgcagtgaag aagtcgatcc tgtccctgag tatcgttctg
     3181 gccctgggcg gctgctcgct gatccccgac taccagcgcc cggatgcccc tgtggccaat
     3241 gcctggccgc agggcgaagc gtatgccgcc agtgccacgg ccgcggcagc cctgcccgag
     3301 tggcgcagct tcttccgcga tccggcgctg cagcagctga tcggtcaggc gctggagaac
     3361 aatcgcgacc tgcgggcggc ggcgctgaac gtcgaggcct accaggcgct gtaccgcatc
     3421 cagcgcgccg agctgttccc gcagctcgac gccacgggca gcggcactcg ccagcgtctg
     3481 ccggccgacc tgacccgcac cggccaggcc gagaccaatg gcgaataccg cgccagcctc
     3541 ggcgtcaccg actgggaagt cgacctgttc gggcgcctgc gcagcctcaa ccaggcggcc
     3601 ctggagcagt atttcgccac cgagcaggcg cagcgcgcga cgcagatcag cctggtggcc
     3661 agcgtggcca atgcctggct ggttctgcag gccgatagcg cgcagctcaa gctgagccag
     3721 gagaccctgc agacctacga gcagagctac aacctgaccc ggcgcagctt cgacgtcggc
     3781 gtggcctcgg cgctggaatt gcagcagtcg cgtaccgtgg tggaaagcgc gcgcagcagc
     3841 gtggcgcagt tcacccgcct gctggcccag gatcgcaacg ccctgcaggt gctgctcggc
     3901 ggcccgctac cggcggatct gccggagcag caggggctgg agggtgcgct gctggccgag
     3961 ctgccggtgg ggttgccgtc cgagctgctg cagcgccgcc cggacatcct ccaggccgag
     4021 cacgcgctgc aggcggccaa cgccaatatc ggcgcggcgc gggcggcgtt cttccccagc
     4081 atcagcctga ctgctcgggc gggcactacc agcgccgagt tgtccggcct gttcaagggc
     4141 ggcagcggca cctggctgtt ccagccgcag atcaacctgc cgatcttcac cgccggcagc
     4201 ctcaaggcca acctcgacta cagccggctg cagaaggacg tccaggtggc gcagtacgag
     4261 cgggccatcc aggtcgcctt cagcgaagtc gccgacggcc tggcggcacg cggcacctac
     4321 cagcagcagc tccaggcgca gcgcgatctg gtcgaggcca gcgccgagta ctaccgcctg
     4381 gccgagaccc gctaccgcac cggcgtcgac agccggctga ccctgctcga cgcgcagcgc
     4441 cagctgttca gcgcccgcca gcaactgatc gacgtccgcc tggcccagct gggcagcgag
     4501 atcgacctgt tcaaggcgtt gggcggcggg gtggagtagg ggcgagactc accctgcgac
     4561 aaggtccggg cgtagggtag aagactcgcg cagcggtctt ctaccgatgc gtccacgcag
     4621 tctgcccggc aggtaatcgc tgcgcgagtt accgacccta cggcttcaaa cgccgcagcc
     4681 cgcgggttac ggcgattggc gctcggcccg gcgtagggta gaagactcgc gcagcgatct
     4741 tctaccgatg cttccacgcg gtctgcccgg taggtaatcg ctgcgcgagt taccgaccct
     4801 acggcttcaa acgccgcagc ccgcgggtta cggcgatggg cgctcggccg ggcgtagggt
     4861 agaagactcg cgcagcgatc ttctaccgat gctttcacgc ggtctgcccg gtaggtaatc
     4921 gctacgcgag ttacctaccc tacgcctggt tcagtcgcgc tccgccagca gcagcaggtt
     4981 gcgcggggtc agctggtagt cgcagaactg gccaagctgc acccggtagc cctgctcctc
     5041 caggtacagc gcacgatcca gcagcagcca cacttccagc ggccggcgga acaggccgcg
     5101 tagcagttcc aggttgcgca cctcggccag acgctgccag ccggcagcct ccagggcgga
     5161 ccagtcgcgg gcgcccggcg ccggcagctg cttgagcagg gcgaggtcgc ggcaccagtc
     5221 ggcgaacggc ttgtgcagcc aggccggcgg cagcgagggt
//
LOCUS       DATCUN010000035         7016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_151587, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000035 DATCUN010000000
VERSION     DATCUN010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151587"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VIO83_01255"
     CDS             complement(<1..398)
                     /locus_tag="VIO83_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEY8329945.1"
                     /translation="MTSMVFYEKPGCASNRRQKQQLRAAGFELQVRDLLGEPWTRELL
                     RPFFAERPVAEWFNPAAPAVKSGAIDPQQLDAEQALALLLAQPLLIRRPLISCGSLRM
                     AGFDPQALNALLPEGDRLEVLAASPEGCAHG"
     gene            complement(411..689)
                     /locus_tag="VIO83_01260"
     CDS             complement(411..689)
                     /locus_tag="VIO83_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY8329946.1"
                     /translation="MALKIVESCVNCWACVDVCPSEAISQGSTHFKISAHKCTECDGD
                     FTEQQCASICPIEGAILLADGSPANPPGSLTGIPPERLAEAMREIQAR"
     gene            complement(727..2238)
                     /gene="nifB"
                     /locus_tag="VIO83_01265"
     CDS             complement(727..2238)
                     /gene="nifB"
                     /locus_tag="VIO83_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HEY8329947.1"
                     /translation="MELSVLGQNDAEQHGAGGCSSGSCGSSDDQLAHLPEHIRDKVHN
                     HPCYSEEAHHYFARMHVAVAPACNIQCHYCNRKYDCANESRPGVVSEVLDPLQAVKKV
                     KAVAATIPQMTVLGIAGPGDPLANPERTFETFRMLSEQAPDIKLCVSTNGLALPDCVD
                     ELAKHNIDHVTITINCVDPEVGAKIYPWIYWNNKRIRGVKAAKILIERQQKGLEMLVA
                     RGILVKVNSVLIPGVNDEHLKEVSRIVKAKGAFLHNVMPLIAEAEHGTFYGVMGQRSP
                     EPEELQDLQDACAGDMNMMRHCRQCRADAVGLLGEDRGDEFTLDKIEEMDIDYAAAMV
                     KRAAIHAAIKEELDDKAAKKARLASLAVPRLEAAQGKAPRPVLMAVATSGGGLINQHY
                     GHATEFLVYEASANGVRFIGHRKVDQYCVGNDSCGEKESALAGSIRALKGCEAVLCSK
                     IGFEPWGLLEEAGIQPNGEHAMEPIEEAVLAVYQEMLQAGRLQQDEAPLRANA"
     gene            complement(2544..4103)
                     /gene="nifA"
                     /locus_tag="VIO83_01270"
     CDS             complement(2544..4103)
                     /gene="nifA"
                     /locus_tag="VIO83_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HEY8329948.1"
                     /translation="MNVTLAKTAPPSRTELYDHQLQAMARISRTLSRVQQIEEILAQI
                     LAVLHNDLGLEHGLVSIGDPQHGALQVGAVHTDSEAVVRACESVRYRIGEGVIGNIFK
                     HGNSVVLGRIAGEPRFLDRLALYDLELPFIGVPIKSHEGNTIGVLVAQPQVRDELLPE
                     RARFMEIVANLLAQTVRLVVNLEDSREVADERDELRREVRGKYSFENMVVGNTPSMRR
                     VFDQVRRVGKWDSTVLVLGESGTGKELIASAIHYNSPRAHQPFVRLNCAALPETLLES
                     ELFGHEKGAFTGAVKQRKGRFEQADGGTLFLDEIGEISPMFQAKLLRVLQEGEFERVG
                     GNQTVRVNVRIVAATNRDLESEVDKGKFREDLYYRLNVMAIRIPALRERSADIPELAE
                     FLLAKIGRQQGRKLSITDGAIRLLMGHRWPGNVRELENCLERSAIMSEDGSIGRDVVS
                     LSGLDHDSLPLAPLPEIDLADEELGDRERVVAALEQAGWVQAKAARLLGMTPRQIAYR
                     IQTLNIHMRKI"
     gene            complement(4121..5686)
                     /gene="nifL"
                     /locus_tag="VIO83_01275"
     CDS             complement(4121..5686)
                     /gene="nifL"
                     /locus_tag="VIO83_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation negative regulator NifL"
                     /protein_id="HEY8329949.1"
                     /translation="MTQAQPTCSTATPAIPAACAELLPEVFRQTAEHAPIAISITDLK
                     ANILYANRAFSSITGYSSEEVIGKNEALLSNGTTPRLVYQALWSRLAQKKPWSGVLVN
                     RRKDGSLYLAELTVAPVFDEHGETLYYLGMHRDSSDLHELEQRVSNQRLMIEAMVNAA
                     PAAMVVLDHQQRVALSNPSFCRLARELTRDGSSEGLVALLRENLASPFAALESQGRAF
                     AGKEISFDLGGHAPRWLSCHGMAIHIEDERAQVFFAPGEERYLLLTLNDISELRQKQQ
                     DSHLNALKALLAEEELLAGMRETFSGAIHRLQGPVNLISAALRMLERRLGDQAGSDPV
                     LAAMREASAAGMEALDSLSGAMPLRPTEAKLPVNVNQLIREVISLCTDRLLAQGIVVD
                     WQPALRLPWVMGVESRLRSMIKQLVDNAIDAMSHNQISHRELLVSTRVEHQQVVRLEI
                     SDSGPGIEPALLLKVFEPFFSTKPPYQAGHGMGLAMVQEIVTEHAGTVHIDSDYHDGC
                     RVVVELPFSAAGN"
     gene            6149..6721
                     /gene="rsxA"
                     /locus_tag="VIO83_01280"
     CDS             6149..6721
                     /gene="rsxA"
                     /locus_tag="VIO83_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HEY8329950.1"
                     /translation="MDYLLFLIGTVLVNNVVLARFLGLCPFMGVSGKLDPSIGMGVAT
                     TLVMTLGGISSWLLEHYVLLPLGIGFLRILAYIVVIAAMVQLIEMIIRKASPELYRAL
                     GIYLPLITTNCAVLGVPLISVREGHDLVQAGLFGFGSALGFTLVMAIFAGLRERLALA
                     SVPAAFSGPPIAFVTAGLLALAFMGFGGLI"
     gene            6765..>7016
                     /locus_tag="VIO83_01285"
     CDS             6765..>7016
                     /locus_tag="VIO83_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEY8329951.1"
                     /translation="MLMATLALAVMGLLLGSGLGVAARKFAVTDANPLLKEIEGLMPG
                     SQCGQCGFPGCAGAAAALVEGNAAVTCCPPGGVALAEKLA"
BASE COUNT          951 a         2346 c         2468 g         1251 t
ORIGIN      
        1 ccgtgcgcgc agccttcggg actggcggcg agcacctcca gtcgatcgcc ctccggcagc
       61 agggcgttga gcgcttgcgg gtcgaagccg gccatgcgca ggctgccgca actgatcagc
      121 ggccggcgga tcagcagcgg ctgggccagc agcagcgcca gcgcctgctc ggcatccagc
      181 tgctgcgggt cgatcgcgcc ggacttgacc gccggcgccg cggggttgaa ccactcggcc
      241 accggccgct cggcaaagaa cggccgcagc agttcgcggg tccagggttc gccgagcagg
      301 tcgcgcacct gcagctcgaa gccggcggcg cgcagctgct gcttctgccg gcggttgctg
      361 gcacagccgg gcttttcgta gaacaccatg ctggtcatgc ccggtccccc ttagcgcgcc
      421 tggatttcac gcatggcctc ggccaggcgt tccgggggga tgccggtcag cgagcccggc
      481 ggattggccg ggctgccgtc ggcgagcagg atggcaccct cgatcgggca gatgctcgcg
      541 cactgctgct cggtgaagtc gccgtcgcac tcggtgcact tgtgcgcact gatcttgaaa
      601 tgcgtgctgc cctgggagat cgcctcgctc gggcagacat ccacgcaggc ccagcagttg
      661 acacaggatt ccactatctt cagcgccatc gcgcacctcc tcgaatcgaa ctgtcgcggg
      721 ccgcgctcag gcgttggccc gcagcggcgc ctcgtcctgc tgcaggcggc cggcctgaag
      781 catttcctga tagaccgcca gcaccgcttc ctcgatgggc tccatggcgt gttcgccgtt
      841 gggctggatg ccggcctcct ccagcaggcc ccacggctcg aagccgatct tcgagcagag
      901 caccgcctcg cagcccttca gcgcgcggat gctgccggcc agcgcgcttt ccttctcgcc
      961 gcagctgtcg ttgccgacgc agtactggtc gaccttgcgg tggccgatga agcgcacgcc
     1021 gttggccgag gcctcgtaga ccaggaactc ggtggcgtgg ccgtagtgct ggttgatcag
     1081 gccgccgccg ctggtggcca ccgccatcag caccgggcgc ggcgccttgc cctgggcggc
     1141 ctcgaggcgc ggcaccgcca ggctggccag gcgcgccttc ttggccgcct tgtcgtccag
     1201 ttcttccttg atcgccgcgt ggatggccgc gcgcttgacc atggcggctg cgtagtcgat
     1261 gtccatctcc tcgatcttgt cgagggtgaa ctcgtcgccg cggtcctcgc cgagcaggcc
     1321 gaccgcgtcg gcgcggcact ggcggcagtg gcgcatcatg ttcatgtcgc cggcgcaggc
     1381 gtcctgcagg tcctgcagct cttccggctc gggactgcgc tggcccatca ccccgtagaa
     1441 ggtgccgtgc tcggcctcgg cgatcagcgg catgacgttg tgcaggaagg cgcccttggc
     1501 cttgacgatg cggctgacct ccttgaggtg ctcgtcgttg acgccgggga tcagcaccga
     1561 gttgaccttc accaggatgc cgcgcgccac cagcatctcc aggcccttct gctggcgctc
     1621 gatgaggatc ttcgccgcct tcacgccgcg gatgcgcttg ttgttccagt agatccacgg
     1681 atagatcttg gcgccgacct ccgggtccac gcagttgatg gtgatggtca cgtggtcgat
     1741 gttgtgcttg gccagctcgt cgacgcagtc cggcagggcc aggccgttgg tggagacgca
     1801 cagcttgatg tccggcgcct gctcgctgag catgcggaag gtctcgaagg tgcgctcggg
     1861 gttggccaag ggatcgccgg ggccggcgat gccgagcacg gtcatctgcg ggatggtcgc
     1921 cgccaccgcc ttgaccttct tcaccgcctg cagcggatcg agcacttcgg acaccacgcc
     1981 gggacgcgac tcgttggcgc agtcgtactt gcggttgcag tagtggcact ggatgttgca
     2041 ggccggcgcc accgccacgt gcatgcgcgc gaagtagtgg tgcgcctcct ccgagtagca
     2101 ggggtggttg tgcaccttgt cgcggatgtg ttccggcagg tgcgccagct ggtcgtcgct
     2161 gctaccgcag gagccggaag agcaaccgcc agcaccgtgc tgctccgcat cgttctgccc
     2221 aagtacgctc agttccatgg tcggtctccg gaggaaagag ggattcacca ttagctgagc
     2281 aagtgccgtg ccgttcgttt atctcattgt ttttaaagaa ttatttgtgg agcttcgagc
     2341 ctgtttgtca ggctgccgac aggggcgcgg gcatggcttt gtcggatagg cgacaagggg
     2401 gcgtcgatat cggggcatgg gcgcggagcg cccgcagccg ggttcccacg cggagcatgg
     2461 gaaccagcga ccgtgaggag agatgatcgc tcccacgctc ccgcgtggga gcgccgcccg
     2521 gcacgctccg gcgcaccgtt gtttcagatc ttgcgcatat ggatgttgag ggtctggatg
     2581 cggtaggcga tctgccgcgg ggtcatgccc agcaggcgcg cggccttggc ctgcacccag
     2641 ccggcctgct cgagggcggc gaccacccgc tcgcggtcgc ccagctcctc gtcggcgagg
     2701 tcgatctccg gcagcggggc gagcggcagg ctgtcgtggt cgagaccgct cagcgagacc
     2761 acgtcgcggc cgatgctgcc gtcctcgctc atgatcgccg agcgctccag gcagttctcc
     2821 agctcgcgca cgttgcccgg ccagcggtgg cccatcagca agcggatggc gccgtcggtg
     2881 atgctcagct tgcggccctg ctggcggccg atcttggcga ggaggaattc cgccagctcg
     2941 gggatgtcgg cactgcgctc gcgcagcgcc gggatgcgga tggccatgac gttgagacgg
     3001 tagtagaggt cctcgcggaa cttgcccttg tccacctcgc tttccaggtc gcggttggtg
     3061 gcggcgacga tgcgcacgtt gacccgcacc gtctggttgc cgccgacccg ttcgaactcg
     3121 ccctcctgca gcacgcgcag cagcttggcc tggaacatcg gcgagatctc gccgatctcg
     3181 tcgaggaaca gggtgccgcc gtcggcctgc tcgaagcggc ccttgcgctg cttgaccgcg
     3241 ccggtgaagg cgcccttctc gtggccgaac agctcggatt cgagcagggt ttccggcagc
     3301 gcggcgcagt tcaggcgcac gaacggctgg tgggcgcgcg gcgagttgta gtggatggcg
     3361 ctggcgatca gctccttgcc ggtgccggac tcgccgagca ccagcaccgt gctgtcccac
     3421 ttgccgaccc ggcgcacctg gtcgaacacc cggcgcatcg acggcgtgtt gcccaccacc
     3481 atgttctcga agctgtactt gccgcgcacc tcgcggcgca gctcgtcgcg ctcgtcggcc
     3541 acctcgcggc tgtcctcgag gttgaccacc aggcgcaccg tctgcgccag caggttggcg
     3601 acgatctcca tgaagcgcgc gcgctcgggc agcagctcgt cgcgcacttg cggctgggcg
     3661 accagcacgc cgatggtgtt gccctcgtgg ctcttgatcg gcacgccgat gaacggcagc
     3721 tcgaggtcgt acagcgccag gcggtcgaga aagcgcggct cgccggcgat gcgcccgagc
     3781 accacgctgt tgccgtgctt gaagatgttg ccgatcaccc cctcgccgat gcggtagcgc
     3841 acgctctcgc aggcgcgtac cacggcttcc gagtcggtgt gcacggcgcc gacctgcagg
     3901 gcgccgtgct gcgggtcgcc gatcgacacc aggccgtgct ccaggccgag gtcgttgtgc
     3961 agcacggcga ggatctgcgc gaggatctcc tcgatctgct gcacccggct gagggtgcgc
     4021 gagatgcgcg ccatggcctg cagctggtgg tcgtacagct cggtccggga cggcggggcg
     4081 gtcttggcaa gcgtcacatt catcggtagg gcctcccgga tcagttgccg gcggccgaga
     4141 agggcagctc gaccaccacc ctgcagccgt cgtggtagtc gctgtcgata tgcaccgtgc
     4201 cggcgtgctc ggtgacgatt tcctggacca tggccaggcc catgccgtgg ccggcctggt
     4261 acggcggctt ggtgctgaag aacggctcga acaccttgag cagcagggcg ggctcgatcc
     4321 ccggcccgct gtcgctgatt tccaggcgca ccacctgctg gtgctccacc cgggtgctga
     4381 ccaacagttc gcggtggctg atctggttgt ggctcatcgc atcgatggcg ttgtcgacca
     4441 gctgcttgat catgctgcgc aggcggcttt ccacccccat cacccagggc aggcgcagcg
     4501 ccggctgcca gtcgacgacg atgccctgcg ccagcaggcg gtcggtgcac aggctgatca
     4561 cttcgcgaat cagctggttg acgttgaccg gcagcttggc ctcggtcggg cgcagcggca
     4621 tggcgccgct gaggctgtcc agcgcctcca tgccggcggc gctggcctcg cgcatggcgg
     4681 ccagcaccgg gtcgctgccg gcctggtcgc cgaggcgccg ttcgagcatg cgcagcgccg
     4741 cgctgatcag gttgaccggc ccctgcaggc ggtggatggc gccgctgaag gtctcgcgca
     4801 tgccggccag cagctcttcc tcggcgagca gcgccttcag cgcattgagg tgcgagtcct
     4861 gctgcttctg gcgcagctcg gagatgtcgt tgagggtgag cagcaggtag cgctcctcgc
     4921 cgggggcgaa gaacacctgg gcgcgctcgt cctcgatgtg gatcgccatg ccgtggcagg
     4981 acagccagcg cggcgcgtgg ccgccgaggt cgaaggagat ctccttgccg gcgaaggcgc
     5041 ggccctggct ttccagcgcg gcgaaggggc tggcgaggtt ctcgcgcagc agcgccacca
     5101 ggccctcgct gctgccatcg cgggtcagct cgcgggccag gcggcagaag ctcgggttgg
     5161 acagcgccac ccgctgctgg tggtcgagca ccaccatcgc cgccggggcg gcgttgacca
     5221 tcgcctcgat catcaggcgc tggttgctga cgcgctgctc cagctcgtgc aggtcgctgc
     5281 tgtcgcggtg catgcccagg tagtagagcg tctcgccgtg ctcgtcgaac accggcgcca
     5341 cggtcagctc ggccaggtac aggctgccgt ccttgcgccg gttgaccagc acgccggacc
     5401 acggcttctt ctgcgccagg cggctccaca gcgcctggta gaccagccgc ggcgtggtgc
     5461 cgttggacag cagcgcctcg ttcttgccga tcacctcctc gctgctgtag ccggtgatgc
     5521 tgctgaaggc gcggttggca tagaggatgt tggccttgag gtcggtgatc gagatggcga
     5581 tgggcgcgtg ctccgccgtc tgccggaata cctcggggag cagctcggcg caggcggcgg
     5641 ggatcgcggg cgtcgcggtg ctgcaggtcg gttgggcctg ggtcatgcgc tttttcctca
     5701 tcactcggcg tcgggtcggg tctgtgcagg gagccggcga acgcgaaaca caccagccgc
     5761 cagggcgtgg ccggcaaagg ccatacgggt catgagggtt ttgcaaacac tgtgccgctg
     5821 cgcaaaaccg ccgcgcggcg cgcgcgggcg ggcaaaagcc gcagggcaga gcggtttcgg
     5881 cgcggttgcc gcggtttttc tgtccgaaag cccacggttg gccggcaatg gccgccgctg
     5941 cctgtcgcaa agccgacaaa agttcgccgc ggccgtcgct gcggcgcggc ctggcgtgcg
     6001 gttccgttga tctggatcag tcttttccgg gcctgggcgg tcgccagggc gctgcgcccg
     6061 gccatggcac gcttgctgca aaaccaccct gcagcaggtg agtgcccgac aagggtgcac
     6121 gccagagtca accgagtggg gtggggcaat ggactatctg ttgtttctga tcggcaccgt
     6181 gctggtcaat aacgtggtgc tggcgcgctt tctcggcctg tgcccgttca tgggcgtgtc
     6241 cggcaagctc gatccgtcga tcggcatggg cgtggccacc accctggtga tgaccctcgg
     6301 cggcatcagt agctggctgc tcgaacacta cgtgctgctg ccgctgggca tcggcttcct
     6361 gcgcatcctc gcctacatcg tggtgatcgc cgcgatggtg caactgatcg agatgatcat
     6421 ccgcaaggcc agcccggagc tgtaccgcgc gctgggcatc tacctgccgc tgatcaccac
     6481 caactgcgcc gtgctcggcg tgccgctgat cagcgtgcgc gagggccacg acctggtgca
     6541 ggccgggctg ttcggcttcg gctcggcgct cggcttcacc ctggtgatgg ccatcttcgc
     6601 cggcctgcgc gagcggctgg cgctggccag cgtgccggcg gcgttttccg gcccgccgat
     6661 cgccttcgtc accgccggcc tgctggcgct ggcgttcatg ggcttcggcg gcctgatctg
     6721 acctgcaact gcgatggccg gccgctgccg gcgaggagag cgagatgctg atggcaaccc
     6781 tggccctggc ggtgatgggc ctgctgctgg gcagcggtct gggcgtggcg gcgcgcaagt
     6841 tcgcggtcac cgacgccaac ccgctgctca aggaaatcga agggctgatg cccggcagcc
     6901 agtgcggcca gtgcggcttt cccggctgcg ccggcgccgc ggcggcgctg gtcgagggca
     6961 atgccgcggt cacctgctgc ccgcccggcg gcgtggccct ggcggagaag ctcgcc
//
LOCUS       DATCUN010000036         4885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_132608, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000036 DATCUN010000000
VERSION     DATCUN010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132608"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VIO83_01290"
     CDS             <1..141
                     /locus_tag="VIO83_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY8329952.1"
                     /translation="RVAPAALDTMRALASELFPQWQDRLAQVSGRPQRPLDPGRPHGE
                     RT"
     gene            125..394
                     /locus_tag="VIO83_01295"
     CDS             125..394
                     /locus_tag="VIO83_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329953.1"
                     /translation="MANAHELARGHKRLRALIEFAVGEGWHVKRTPGGHLKFTKAGCA
                     AIYTSSTASDHRAELNARAQIRRAEREARMAPAGGRGDCEGCGHG"
     gene            387..2066
                     /locus_tag="VIO83_01300"
     CDS             387..2066
                     /locus_tag="VIO83_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HEY8329954.1"
                     /translation="MADMTSQDMAGKLLAAGFERSGPAATALSDPIADTPMVVTLDQL
                     RPYDHDPRMKRNPAYEEIKASIRERGLDAAPAITRRPGEDHYIIRNGGNTRLAILREL
                     WSETKEERFFRISCLFRPWPERGEIVALTGHLAENELRGGLTFIERALGVEKAREFYE
                     QESGAALSQSELARRLAADGFPVQRSHITRMADAVRYLLPAIPTVLYGGLGRHQVERL
                     SVMRTACKRTWEHYAKDRSLPLDFDSFFQEVLAQFDTQGDEFAPQRVQDELIGQMSEL
                     LGIGYDVLALDLTESESRHRALVSDPTPPAAQPALPSPTAGTPTPPGAASSIATPAAA
                     AGPPPASPPASEPTLRHDDTAVGEAATGSQPAAPGDLLREHIVSPAPTTERLQSIQRM
                     VADQLGDALPPDFSASVLQSIPVQAGGLYPISDVWYIDAGLDTPDRLRIHIAQFAREI
                     AGEAGLDECIEDRADGIGFACRARGQAPSPLGRAVLTLLTSLTGQPVAEAGLDGAQLA
                     ADLPTLLHGQSQGHGSGARRLSDTALVKLFRLLRLARRLLDLEAGTTAPGT"
     gene            2081..2641
                     /locus_tag="VIO83_01305"
     CDS             2081..2641
                     /locus_tag="VIO83_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017513942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HEY8329955.1"
                     /translation="MSTSHPLNQAVIAQALYDLRNGQLRRCKAMGFGEAELDALKHPA
                     LISVLANASVSWCSVTVNREVLQRLLNQAQDVEKEIATVDRMLRLGASTEMVSRFYGL
                     THQEVALRREILGLPKRKGRHPVLDETQDTELWRQWKAVTSSRNVDLEDETSILDAAM
                     DLAEGMSLPLSVVWAAIRNWVDQGLG"
     gene            2645..3883
                     /locus_tag="VIO83_01310"
     CDS             2645..3883
                     /locus_tag="VIO83_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003158470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HEY8329956.1"
                     /translation="MAVDDTAPRAQRPGPIALADLFDAALKDLAPKSSSGAPASLPAP
                     TPTPPTSGDAFLFSGNRHESVPRRLFLDRRLTPLERNAWQVFRLMLNDDGVTAFPTYE
                     QLRPWLASMPCAGQASHETVARALTLLRLTRWLSLVRRRRDPKTGRILGNLYVLHDEP
                     LTPFEAMQLDADYLALVSQSLGHSAKAVQVVGLNTLQEIADDPMLSGRTLPSRLQVLA
                     ERLADQGITASESYPQEDAVHDSEEGPASLLRNGDRPSSESEAGPKPAPDGALRNSKQ
                     DRTVRSSRIDEVRTTAQARALGDLQWPKRFAELKAEQQAGAKMALQQVDAALRQAVLD
                     EWAARCGKHGIRNPAGYLFGIIQRAMRGEFNAWAKQTGPAPPTPSAARAPPTEPPRNV
                     VPPEVARQHIERLRDLLRKS"
     gene            4313..>4885
                     /locus_tag="VIO83_01315"
     CDS             4313..>4885
                     /locus_tag="VIO83_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03761 family integrating conjugative element
                     protein"
                     /protein_id="HEY8329957.1"
                     /translation="MATSNEPLQLNLGSLRSAMSLTLHTHHASRIWHGRAAAEGRPGI
                     VGLNGYIAVMNKMKRGSEQDDPYSDWWMLRIEDKLDQTRTTLQTLRDQVDQALAGVPA
                     ALTLGENLNVQPVKLPLFVNAQLGFAAVYLLADYDDIARKLILAHHTALIDRSTLERW
                     LNEGAHALRSLFSLAQQYRYSGCTRDDFAAK"
BASE COUNT          847 a         1729 c         1602 g          707 t
ORIGIN      
        1 agagtcgccc ctgccgcgct cgacacgatg cgcgcgctcg ccagcgagtt gttcccgcaa
       61 tggcaggacc gactggctca agtatccggc cgcccgcagc gacctcttga tcctgggagg
      121 ccccatggcg aacgcacatg agctggcccg cggccacaag cggctgcgcg ccctgatcga
      181 gttcgcggtc ggtgagggct ggcacgtcaa gcgcacgccg ggcgggcacc tcaagttcac
      241 caaggccggc tgcgcggcga tctacacgag ttcgacagcg agtgaccacc gggccgaatt
      301 gaacgcccgt gcgcagatcc gccgcgccga gcgagaggcc cgcatggcgc cggccggcgg
      361 tcgtggggac tgcgaggggt gcggccatgg ctgacatgac ctcgcaggac atggcaggca
      421 agctgctcgc ggccggcttc gagcgcagcg gcccagcggc tacggccttg agcgacccga
      481 tcgcggacac gcccatggtc gtgacgcttg accagttgcg gccctacgac catgacccgc
      541 gcatgaagcg caacccggct tacgaggaaa tcaaggcatc catccgcgag cgcggcctgg
      601 acgcggcgcc ggccatcacg cgcaggcccg gcgaggacca ctacatcatc cgcaacggcg
      661 gcaacacgcg actggcgatc ctgcgcgaac tctggtcgga gaccaaggaa gaacgcttct
      721 tccgcatatc gtgcctgttc cgaccatggc cggagcgcgg tgaaatcgtc gcgctgaccg
      781 ggcatcttgc cgagaacgaa ctgcgcggtg gcctcacgtt catcgagcgc gcgctcggcg
      841 tcgagaaagc acgggagttc tacgagcagg agagcggcgc cgccctgagc cagtcggaac
      901 tggcccgccg cctggcggcc gatggcttcc ccgtccagcg gtcgcacatc acccgcatgg
      961 ccgacgcggt gcgctacctg ctgcccgcca ttcccactgt gctctatggc ggcctggggc
     1021 gccaccaggt cgagcggctg tcggtcatgc gcacggcctg caaacgcacc tgggagcact
     1081 acgccaagga ccgttcgctg ccgctggact tcgacagttt ctttcaggag gtgctggcgc
     1141 agttcgacac gcagggcgac gagttcgcgc cgcagcgcgt gcaagacgag ctgatcggcc
     1201 agatgtccga gctgctgggc atcggctacg acgtgctggc gctggacctg accgaatcgg
     1261 aaagccgcca ccgggcgctg gtcagtgacc cgacgccacc tgcggcgcag ccggcgttgc
     1321 cttcgcccac cgccggcacg ccgactccgc cgggcgcagc atcgtccatc gccacgcctg
     1381 cagcggcggc cggtcctcca cccgccagcc ctccggcgtc cgagcccacc ttgcgccacg
     1441 acgacacggc ggttggcgaa gcggccacgg gaagccagcc ggccgcaccg ggcgatctgc
     1501 ttcgcgagca catcgtctcg ccagcaccga cgacagaacg gctccaatcg atccagcgca
     1561 tggtcgccga ccagttgggc gatgcgctgc cgcccgactt ctcggcgagc gtgctgcagt
     1621 ccatcccggt gcaagccggc ggactctatc cgatctccga cgtctggtac atcgacgccg
     1681 gcctggacac gcccgatcgg ctgcgcatcc acatcgcgca gttcgcgcgc gagatcgccg
     1741 gggaggcagg cctggacgag tgcatcgagg atcgcgccga tggcatcggg tttgcgtgcc
     1801 gcgcccgcgg ccaagccccg tcgccgctcg gccgcgcggt gctgacgctc ctcacttccc
     1861 tgacgggtca gcccgtcgcc gaagcgggcc tggacggcgc gcagctcgcc gccgacctgc
     1921 cgaccctgtt gcacggccag agccaaggcc atggcagcgg cgcaaggcgc ttgagcgaca
     1981 cggcgttggt caagctcttt cggctgctgc gcctcgcccg gcgtctgctg gacctggaag
     2041 ccggcaccac ggcgcccggg acgtgagcga gggaggccgc atgtccacat cgcatccgct
     2101 caaccaggcc gtgattgccc aggcgctgta tgaccttcgc aacggccaac tgcgccgctg
     2161 caaggccatg ggcttcggcg aggccgagct ggatgcgctc aagcaccccg cgctgatcag
     2221 cgtgctggcc aacgccagcg tctcgtggtg ctcggtcacg gtcaaccgcg aagtgctgca
     2281 gcgcctgctc aaccaggcac aggacgtgga gaaggaaatc gccacggtcg atcgcatgct
     2341 gcggctgggg gccagcacgg agatggtcag ccgcttctat ggcctgaccc atcaggaagt
     2401 cgccctgcgg cgggagatcc tcggcctgcc caagcgcaag gggcgccacc ccgtcctgga
     2461 cgagacccag gacaccgaac tgtggcggca atggaaagcc gtgaccagca gcaggaacgt
     2521 cgatctggag gacgagacct cgattctcga tgcggccatg gacctggccg agggcatgtc
     2581 gctgccgctc tcggtggtct gggccgcgat caggaactgg gtcgatcagg gattgggcta
     2641 ggccatggcc gtggacgaca ccgccccacg agcccagcgc cctggcccca tcgcactcgc
     2701 cgacctgttc gacgctgcgc tgaaggacct cgcgcccaag tccagctctg gcgctcccgc
     2761 gtccctgcct gctccaacgc ccacgcctcc tacgtccggc gacgccttcc tgttcagtgg
     2821 caatcggcac gagagcgtgc cgcggcggct gttcctcgac cgccgcctga caccgctgga
     2881 gcgcaacgcc tggcaggtgt tccgattgat gctcaacgac gatggcgtca cggcatttcc
     2941 cacctatgag cagttgcgcc cctggctggc gtcgatgccc tgcgcaggcc aggcctccca
     3001 tgagaccgtg gcgcgggcgc tgacgctgct gcgcctgacg cgctggctga gcctcgtgcg
     3061 acgacggcgc gaccccaaga ccggtcgcat cctcggcaac ctctacgtcc tgcacgacga
     3121 accgctgacg ccgttcgagg cgatgcaact cgacgccgac tacctggccc tggtcagcca
     3181 gtcgctgggg cattcggcca aggccgtcca ggtggtggga ctcaacaccc tccaggagat
     3241 cgcggacgac ccgatgctgt ccggacgcac cttgccgtcg cggctgcaag tgctcgccga
     3301 gcgcctggcc gaccagggca tcaccgcctc cgaaagttat ccacaggagg atgcggtcca
     3361 cgattccgaa gaagggcccg cgagccttct tcggaatggc gatcgcccat cttcggaatc
     3421 cgaagcaggg ccgaaacccg cgccagacgg cgctcttcgg aattcgaagc aggaccgtac
     3481 agtacgtagt agtcgtattg atgaagtacg tactaccgcg caggcgcgtg cgctgggcga
     3541 cctgcaatgg cccaagcgct tcgcggaact gaaggcggaa cagcaggccg gagccaagat
     3601 ggcgttgcag caggtggacg ctgccctgcg gcaggccgtg ctggacgaat gggccgcacg
     3661 gtgcggcaag catggcatcc gcaaccccgc cggttatctg ttcggcatca tccagcgcgc
     3721 catgcgcggc gagttcaatg cctgggccaa gcagaccggc ccggcaccgc caacaccatc
     3781 ggcggcgcga gcgccaccaa ccgagccgcc gcgcaacgtc gtgccgcccg aggtggcccg
     3841 gcagcacatc gagcgcctgc gcgatctgct gcgcaaatcc tgagcacagg gcttgtcaaa
     3901 gccgtgaagt agatctccat ggcggtcggc agaaacggtc ccctggggac ggctgtgcgg
     3961 tgaagcgccg ggcgacggcg cgacatgctg cgcaaaacct gagtagagcg catgcaagac
     4021 catgaagcag acctccatgg cgctcaacgg aacggtcccc tggggacggc tgcgcggcga
     4081 aggatcgtgt cgaacggcag cgcgacgtgc ggtgcagcac gcataccgtc aacaaccgag
     4141 gacgggaacg ccgatgtgag tgcccatctg cggagcggtc cccaggggac cgttgcgcgc
     4201 tgcgccgcca gcgcgcacag aaccgggggt cggctcattt cggtttgttg actgactgcc
     4261 ttccgctgca tgcggatgct ggcggctccc tgtccaccag cgagcgatca ccatggcaac
     4321 cagcaacgaa ccattgcaac tcaacctcgg ctccctgcgc agcgcgatgt cgctgacgct
     4381 gcacacccac cacgcctcgc gcatctggca tggccgcgcc gccgccgagg ggcgaccggg
     4441 catcgtcggc ctgaacggct acatcgccgt catgaacaag atgaagcgcg gctcggagca
     4501 ggacgaccca tacagcgact ggtggatgct tcgcatcgaa gacaagctcg accagaccag
     4561 gaccacgctg cagacgctgc gcgaccaggt ggaccaggcc ctggccggcg tgcccgccgc
     4621 gttgaccctg ggcgagaacc tcaacgtgca gcccgtcaag cttccgctgt tcgtcaatgc
     4681 gcagttgggc tttgccgccg tctatctcct ggccgactac gacgacatcg cgcgcaagct
     4741 gatcctcgcc caccacaccg cgctgatcga tcgctcgacg ttggagcgct ggctcaacga
     4801 aggtgcccac gcgctgcgca gcctgttcag cctggcccag caataccgct actcgggctg
     4861 cacccgcgac gacttcgcgg ccaag
//
LOCUS       DATCUN010000037         2919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_132731, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000037 DATCUN010000000
VERSION     DATCUN010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132731"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VIO83_01320"
     CDS             complement(<1..139)
                     /locus_tag="VIO83_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HEY8329958.1"
                     /translation="MTESFVTQCPHCQTRFRVTRAQLGMARGAVRCGACLQVFNAAEQ
                     LA"
     gene            complement(202..1080)
                     /gene="prmA"
                     /locus_tag="VIO83_01325"
     CDS             complement(202..1080)
                     /gene="prmA"
                     /locus_tag="VIO83_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY8329959.1"
                     /translation="MPWLQVRLAITPHQAAVLEDQLLELGAVSVTFMDAEDQPIFEPD
                     LGTTPLWTHTHLLALFEDGTDADAVLAHLKLLRGGELPEYQVERIEDQDWERSWMDNF
                     HPMRFGRRLWIVPSWHEAPEPDAVNLLLDPGLAFGTGTHPTTALCLEWLDGQDLAGCR
                     VLDFGCGSGILGIAALLLGAESALGTDIDPQALEASLDNAGRNGLPADAFPVYLPADL
                     PPQPVDVVVANILAGPLVQLAPTITALVKSGGRLALSGILAEQAEEVRAAYADAFDLD
                     PTAVKEGWVRISGVRR"
     gene            complement(1291..2640)
                     /gene="accC"
                     /locus_tag="VIO83_01330"
     CDS             complement(1291..2640)
                     /gene="accC"
                     /locus_tag="VIO83_01330"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY8329960.1"
                     /translation="MLQKVLIANRGEIALRVLRACKELGIKTVAVHSTADRDLMHVSL
                     ADESVCIGPASSAQSYLSIPAIIAAAEVTGADGIHPGYGFLAENADFAEQVEKSGFTF
                     IGPTADVIRLMGDKVSAKDAMKKAGVPTVPGSAGPLPEDEEEALKIAREVGYPVIIKA
                     AGGGGGRGMRVVHKEEDLIDSAKLTRNEAGSAFGNPMVYLEKFLTNPRHVEIQVLSDG
                     QGNAVHLGDRDCSLQRRHQKVIEEAPAPLIDEEARRKVQAHCVQACIDIGYRGAGTFE
                     FLYENGSFYFIEMNTRVQVEHPVTEMVTGVDIVKEMLRIGGGEKLSITQDDVVIRGHA
                     IECRINAEDPKTFMPSPGKVKHFHAPGGNGVRVDSHLYDGYSVPPYYDSLIGKLITFG
                     ATRDEALGRMRNALDELIVDGIKTNAPLHAELVRDPGFCKGGVNIHYLEKKLGMDKH"
     gene            complement(2659..>2919)
                     /gene="accB"
                     /locus_tag="VIO83_01335"
     CDS             complement(2659..>2919)
                     /gene="accB"
                     /locus_tag="VIO83_01335"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY8329961.1"
                     /translation="AAPAAPALAGHVVRSPMVGTFYRASSPDAKPFADVGQTVKKGDI
                     LCIVEAMKMMNHIEAEASGTIGSVLVENGHPVEFDQPLFTIV"
BASE COUNT          447 a          920 c         1057 g          495 t
ORIGIN      
        1 tggccagctg ctcggcggcg ttgaacacct gcaggcaggc gccgcagcgc acggcgccac
       61 gggccatgcc cagctgcgcg cgggtgacgc ggaagcgggt ctggcaatgg gggcactggg
      121 tgacgaatga ctcggtcatg ctgcgctatt ccggaacagg tcaggggttg attctagcgc
      181 agcgcagccg gcctcggcag ctcagcggcg cacaccgctg atgcgcaccc agccttcctt
      241 caccgcggtc gggtcgaggt cgaaggcatc ggcgtaggcg gcgcgcactt cctcggcctg
      301 ctcggcgagg atgccggaca gcgccaggcg cccgccgctc ttgaccaggg cggtgatggt
      361 cggcgccagc tgcaccagcg ggccggcgag gatgttggcc accaccacgt cgaccggctg
      421 cggcggcagg tcggccggca ggtagaccgg gaaggcgtcg gccggcaggc cgttgcgccc
      481 ggcattgtcc agggaagcct ccagcgcctg cgggtcgatg tcggtgccca gcgccgattc
      541 ggcgccgagc agcagcgcgg cgatgccgag gatgccggag ccgcagccga agtcgagcac
      601 gcggcagcca gcgaggtcct ggccgtccag ccactccagg cacagcgcgg tggtcgggtg
      661 ggtgccggtg ccgaaggcca ggcccggatc gagcagcagg ttgaccgcgt ccggctccgg
      721 cgcttcgtgc cagctcggca cgatccacag gcggcggccg aagcgcatcg ggtggaagtt
      781 gtccatccag ctgcgctccc agtcctggtc ctcgatgcgc tcgacctggt actcgggcag
      841 ctcgccgccg cgcagcagct tgaggtgggc gagcaccgcg tcggcgtcgg tgccgtcctc
      901 gaacagggcg agcaggtggg tatgggtcca cagcggggta gtgcccaggt ccggctcgaa
      961 gatcggctgg tcctcggcgt ccatgaaggt cacggagacc gcacccagct ccagcagctg
     1021 gtcttccagc acggcggcct ggtggggagt gatggcgagt cggacttgca gccagggcat
     1081 ggcggaggcc tcttgaagat gggcgggcga aggcgggcaa gcttactgga gggggcgggg
     1141 gctgtgccag catgaaggta gcccgctgta ggggcgaatt cattcgcctg gcagggccgc
     1201 gttggcgaat gaattcgccc ctacggcaat gtcccgtgaa acgacagagg ccgcccgaag
     1261 gcggcctctg tgcaagcagc gagtcgagac tcagtgcttg tccataccca gttttttttc
     1321 caggtagtgg atgttgacgc cgcccttgca gaagccgggg tcgcgcacca gctccgcgtg
     1381 cagcggggcg ttggtcttga tgccgtcgac gatcagctcg tcgagggcgt tgcgcatgcg
     1441 gcccagggcc tcgtcgcggg tggcgccgaa ggtgatcagc ttgccgatca gcgaatcgta
     1501 gtacggcggt accgagtagc cgtcgtacag gtgcgaatcg acgcgcacgc cgttgccgcc
     1561 cggagcgtgg aagtgcttga ccttgcccgg gctgggcatg aaggtcttcg ggtcctcggc
     1621 gttgatccgg cactcgatgg catgaccgcg gatcaccacg tcgtcctggg tgatcgacag
     1681 cttctcgccg ccgccgatgc gcagcatctc cttgacgatg tcgacgccgg taaccatctc
     1741 ggtgaccggg tgttccacct gcacgcgggt gttcatctcg atgaaataga aactgccgtt
     1801 ttcatagagg aactcgaagg tgccggcgcc gcggtagccg atgtcgatgc acgcctgcac
     1861 gcagtgggcc tggaccttgc ggcgggcttc ctcgtcgatc agcggcgcag gcgcctcctc
     1921 gatcaccttc tggtggcggc gctgcagcga gcagtcgcgg tcaccgaggt gcacggcgtt
     1981 gccctggccg tcggacagga cctggatttc cacgtggcgc ggattggtca ggaacttctc
     2041 gaggtagacc atcgggttgc cgaaggcgga accggcttcg ttgcgggtca gcttggccga
     2101 gtcgatcagg tcctcttcct tgtggaccac gcgcatgccg cgaccaccgc cgccgccagc
     2161 ggccttgatg atcaccggat agcccacttc gcgggcgatc ttcagcgctt cttcctcgtc
     2221 ttccggcagc gggccggcgg agccgggcac ggtcggtacg ccggccttct tcatggcgtc
     2281 cttggccgag accttgtcgc ccatcaggcg gatcacgtcg gcggtcgggc cgatgaaggt
     2341 gaagccggat ttctccacct gctcggcgaa gtcggcgttc tcggcgagga agccgtagcc
     2401 cgggtggata ccgtcggcgc cggtgacttc ggcggcggcg atgatcgccg ggatgctcag
     2461 gtaggactgg gcggaggacg ccgggccgat gcagacagac tcatcggcca gcgacacgtg
     2521 catcaggtcg cggtcggcag tcgagtgcac agccacggtc ttgatgccca gttccttgca
     2581 cgcgcgcagc acgcgcaggg cgatctcgcc gcggttggca atcaggactt tttgcagcat
     2641 cgcaggaatc tccgcgtgtc agacgatggt gaacagcggc tggtcgaact cgaccggatg
     2701 gccgttttcc accagtaccg agccgatggt gccgctggct tcggcttcga tgtggttcat
     2761 catcttcatc gcttcaacga tgcacaggat gtcgcccttc ttcacggtct ggccgacgtc
     2821 ggcgaacggc ttggcgtccg gcgaggaggc gcggtagaag gtgccgacca tcggcgagcg
     2881 caccacgtgg ccggccaggg ccggggcagc gggggcggc
//
LOCUS       DATCUN010000038         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_170470, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000038 DATCUN010000000
VERSION     DATCUN010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170470"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1558
                     /gene="vgrG"
                     /locus_tag="VIO83_01340"
     CDS             <1..>1558
                     /gene="vgrG"
                     /locus_tag="VIO83_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HEY8329962.1"
                     /translation="RIFFNHTRHKFAIRWFDAAFDVLAFEGEEHLSQPFTYRIEFTCS
                     EQDLSAEKMLNKDASFSLYPPPAKPPYPGLPVPEVKPLRSLYGMVNSFQRLSASRDEA
                     RYEITLQPRLALLDRGRQYRLYQQQSAPEIVEHILRSRHDFLGQHFLFQLVREYPRRE
                     QVMQYGESDLAFIERLLAEVGIWYRFTHDDRLRIDVVEFHDDQRHYQFGVKLPLRSPA
                     GFASGEDGVWGLQTRHQVVEKSINFRAYHPLDARAFLDGEVDQTRGTTTSTYGEAYHY
                     AEPYRVLGDKLDQDEDLLSESGFFYARLRHERYLNSQTHLSGASSSATLAPGQVLKIA
                     GAPQAFAPGAVITGLRTRAARDRNIEVHFEAIPYAEMVCFRPKVPPKPIIAGTLPARL
                     SSPERNDRYAEIDLEGRYRVRFLFDRDIWKPGQESTWLRLARPYGGDTHGLHLPMLAG
                     TEVAVAFEQGDPDRPYIAHALHDSEHPDHVTLRRRDHTRNVLRTPANNKLRMEDLRGQ
                     EHVKLSTEYGG"
BASE COUNT          305 a          578 c          429 g          246 t
ORIGIN      
        1 cgcgcatctt cttcaaccac acccgtcaca aattcgcgat ccgctggttc gacgccgcgt
       61 tcgatgtcct cgccttcgaa ggcgaagaac acctcagcca acccttcacc taccgcatcg
      121 aattcacctg cagcgagcaa gacctcagcg ccgagaagat gctcaacaag gacgccagtt
      181 tcagcctcta tccgccgccg gcaaagcctc cttaccctgg cctgccagtg cccgaggtca
      241 agccgttgcg cagcctgtat gggatggtca atagcttcca gcgcctgtcc gcctcccgcg
      301 acgaagcgcg ctatgaaatc accctgcaac cgcgccttgc cctgctcgat cgtggccgtc
      361 agtaccgcct gtaccagcag cagtcggcgc cggagatcgt cgagcacatc ctgcgcagcc
      421 gccacgattt cctcggccag cacttcttgt tccaattggt gcgcgaatac ccccggcgcg
      481 agcaggtcat gcagtacggc gaaagcgacc ttgcgttcat cgagcggctg ctggccgagg
      541 tcggcatctg gtaccgcttc acgcatgacg accgcctgcg gatcgacgtc gtcgagttcc
      601 acgatgacca gcgccactac cagtttggcg tgaaattgcc actgcgctca ccggccggtt
      661 tcgccagcgg tgaggacggc gtgtggggtt tgcagacccg ccaccaggtg gtggaaaaat
      721 ccatcaactt tcgcgcctat cacccccttg atgcccgcgc ctttctcgat ggcgaggtcg
      781 accagacccg tggcaccacg accagcacct acggcgaggc ctaccactac gccgaaccct
      841 atcgcgtgct cggtgacaaa ctggaccagg acgaagacct gctaagcgaa agcggctttt
      901 tctacgcgcg cctgcgccac gagcgctacc tcaacagcca gacccatctc agcggcgcca
      961 gcagcagcgc caccctggcg ccaggccagg tgctgaaaat cgccggcgcg ccccaggcct
     1021 ttgcccctgg cgcggtgatc accggcctgc gcacccgtgc cgcccgcgac cgcaacatcg
     1081 aagtgcattt cgaagccatt ccctacgccg agatggtctg cttccgccca aaggtcccgc
     1141 ccaaaccgat catcgccggc accctgccag cccggctgag cagccccgag cgcaacgacc
     1201 gctacgccga aatcgatctc gaaggccgct accgcgtgcg cttcctgttc gaccgcgaca
     1261 tctggaaacc cggccaggaa agcacctggc tgcgcctggc ccggccttac ggcggcgaca
     1321 cccacggcct gcacctgcca atgctcgccg gcaccgaagt ggcggtggct ttcgaacagg
     1381 gcgacccgga ccgcccctac atcgcccacg ccctgcacga cagcgaacac ccggaccacg
     1441 tgaccctgcg caggcgcgac cacacccgca atgtgctgcg tacaccggcg aataacaagc
     1501 tgcgcatgga agacctgcgg gggcaggagc acgtcaagct cagcaccgaa tatggcgg
//
LOCUS       DATCUN010000039         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_208082, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000039 DATCUN010000000
VERSION     DATCUN010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208082"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..687
                     /locus_tag="VIO83_01345"
     CDS             271..687
                     /locus_tag="VIO83_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY8329963.1"
                     /translation="MRTCHRIVSGVLAATLLAQLAGCGTLFYPERRGQLNGQIDPAIA
                     ALDAIGILFYVIPGLIAFAVDFGTGAIYLPEGRYSVAPERLQDALGSDGRVDHVRLRA
                     ILQEELARDLPLDDPRLIRHSGNLEQLAAYGLRPSA"
     gene            699..1571
                     /locus_tag="VIO83_01350"
     CDS             699..1571
                     /locus_tag="VIO83_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY8329964.1"
                     /translation="MNLSREHGRLMRLASGAALATALVLIAAKAFAWWLSGSVSLLAG
                     LTDSLLDGAASLLNLIAVNYALRPADDDHRYGHGKAEALAGLGQAVFIAISAVLVGIR
                     GFERLQAPQEITATGIGVAVMLLSLAMTALLLVFQRHVVKVTGSTAIRADSLHYASDL
                     LLNTSILAALLLAAYGWQRADALFGIAIAAYILWSALTIVRESAAVLMDKELPLEISE
                     RMTQLALNVPGVLGAHDLRTRMSGTRWFVQLHLDLPASLSLHEAHDKCVAVEQIIREE
                     FPRAEVLVHADPQH"
     gene            1948..>2319
                     /locus_tag="VIO83_01355"
     CDS             1948..>2319
                     /locus_tag="VIO83_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY8329965.1"
                     /translation="MSKLDRYDLRILAELQRDARISNQELAERIGLSPSPCSRRVKQL
                     EDDGYILRQVALLDRKKLGLTLTVYVLVGMDRHTPERFEHFESAIRQCPQVLECSLVT
                     GMEADYQLKVVVPDMDHYQQFL"
BASE COUNT          361 a          839 c          747 g          372 t
ORIGIN      
        1 gcggcacgcg ggtggtcttg ccggcgccgg gcggcgcctc cagcaccact tcgtggcggc
       61 gggcgagcgc ctcgcgcagc gcgggcagga cggcatcgat gggcagggcg gacagactca
      121 tgggggctcg caacggcaga cggcaagggc ggcgattata gcggcgaagc gccagcgcct
      181 tccgggtgtc atggccgaac gcttacacta tgcccccgcg tctggcaccg cactgccacg
      241 ctgcagcagc gccacccaag gagaagatgc atgcgcacct gtcaccggat cgtcagcggc
      301 gtcctcgccg ccaccctgct cgcccagctc gcaggctgcg gcaccctctt ctatccggag
      361 cgccgcggcc agctcaacgg gcagatcgac ccggccatcg cggcgctgga tgccatcggc
      421 atcctgttct atgtgatacc cgggctgatc gccttcgccg tcgacttcgg caccggcgcc
      481 atctacctgc cggagggccg ctactccgtg gcgcccgagc gcctgcagga tgccctcggc
      541 agcgacggcc gtgtcgacca cgtccgcctg cgcgccatcc tgcaagagga gctggcccgc
      601 gacctgccgc tggacgaccc gcgcctgatc cgccatagcg gcaatctcga acaactggcc
      661 gcctacggcc tgcgcccaag cgcctgagga gaactgcaat gaacctgagc cgcgaacacg
      721 gccgtctgat gcgcctggcc tcgggcgccg ccctggccac cgcgctggtc ctgatcgccg
      781 ccaaggcctt cgcctggtgg ttgagcggct cggtcagcct gctcgccggc ctcaccgatt
      841 cgctgctcga cggtgccgcc tcgctgctca acctgatcgc ggtgaactac gcgctgcgcc
      901 cggcggacga tgaccaccgc tacggtcatg gcaaggccga ggcgctggcc ggcctcggcc
      961 aggcggtgtt catcgccatc agcgcggtgc tggtcggcat ccgcggcttc gagcgtctgc
     1021 aggccccgca ggagatcact gccaccggca tcggcgtggc ggtgatgctg ctgtcgctgg
     1081 ccatgaccgc cctgctgctg gtattccagc gccatgtggt caaggtcacc ggctccaccg
     1141 cgatccgcgc cgactcgctg cactacgcct ccgacctgct gctcaacacc agcatcctcg
     1201 ccgccctgct gctggccgcc tacggctggc agcgcgccga tgcgctgttc ggcatcgcca
     1261 tcgccgccta catcctgtgg agcgccctga ccatcgtccg cgagtcggcg gcggtgctga
     1321 tggacaagga gctgccgctg gaaatcagcg agcgcatgac ccagctggcc ctcaacgtgc
     1381 ccggcgtgct cggcgcccac gacctgcgta cgcgcatgtc cggcacccgc tggttcgtcc
     1441 agctgcacct tgacctgccg gccagcctga gcctgcacga ggcgcacgac aagtgcgtgg
     1501 cggtcgagca gatcatccgc gaggaattcc cccgcgccga agtgctggtg cacgccgacc
     1561 cgcaacattg agccatcccc ccttctgccc cctgacgcta ccggtctggc cggccgcgct
     1621 ggcgggcagc aatcacggga cataaaaaag gaggccttgc ggccccctcg ggatgtttcg
     1681 tccggatcgc gactgcggta acagcctgct cggcgcaacg cctggtgggc agtgcgggcc
     1741 tggggccgca accgggtggg ttgccggcag cgcggtgcct gattgggctc tgcgcgggag
     1801 tgagcgtccg ggccttgaaa ctgattcaaa ctgtacgcgc tttcgtgcag caggagtttg
     1861 cgaagattgt cccctgaaac atgacttgcg caagcaaatg ccaatccagc atgcttctgc
     1921 gcaatttcat caaaccggcc ggccctcatg agcaaactcg atcgctacga cctgcgcatc
     1981 ctcgccgagc tgcagcgcga cgcgcggatt tccaatcagg agctggccga gcgcatcggc
     2041 ctgtcgccct cgccctgttc gcgacgggtc aagcagctgg aggacgacgg ctacatcctg
     2101 cgccaggtcg ccctgctcga ccgcaagaag ctcggcctga ccctcaccgt gtacgtgctg
     2161 gtgggcatgg accgccacac cccggagcgc ttcgagcact tcgagtcggc catccgccag
     2221 tgcccgcagg tgctcgaatg cagcctggtg accggcatgg aggcggacta ccagctcaag
     2281 gtggtggtgc cggacatgga ccactaccag cagtttctg
//
LOCUS       DATCUN010000040         8827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_264470, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000040 DATCUN010000000
VERSION     DATCUN010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8827
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264470"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VIO83_01360"
     CDS             complement(<1..1193)
                     /locus_tag="VIO83_01360"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEY8329966.1"
                     /translation="MSKGLKRGLAALVLAPLLYALLGFFLLPWAGLKLANQKLAEYAT
                     LPASLERIEFNPFSLELTLHQLRLGAPGAEQLAFSRLYANLQVDSLWQGALHLLELQL
                     EQPHVELLFATDRTFNLLQLFRLPPAASAAEAQPAGELFPLHVDRLQLSGGSLHLNDL
                     RPQPSVEVRYENLAIELLDFGTRAEDQGQLRLTASGSSGASVSLQALLSLQPLRAEGH
                     LSITDIALKSWWPYVRRLLPLELEQGVASLASDYRLELKDGLQLQLDNSQLQLNGLQV
                     HEADGRPLLDLQQLEATAERLEFHPGPQLQLSNGELRLATLTLHGLAEQPPLKLDSLA
                     VTGTALNLAERQVVIGQLHSQGLEARAAREADGRLDWQALLDRQLAALEQHRAPRAQT
                     LDQPAV"
     gene            complement(1346..2362)
                     /locus_tag="VIO83_01365"
     CDS             complement(1346..2362)
                     /locus_tag="VIO83_01365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8329967.1"
                     /translation="MSALNQALCAALERRQALFAELHAQGTDCYRLFHGSQEGAPGLT
                     VDRYGPQLLVQSFHQPLERDALLQLLDSCQQALATPLLPVYNDRSQPHSRIDRRDPVY
                     QATPEALADLVGHEWGLNYRVRARHAGQDPLLFLDLRNARGWVKRHSAGKSVLNLFAY
                     TCGVGLCAAAGGARSVTNLDFAEGNLAVGRENAALNPDLASMRFVASDYFPAIRQFAG
                     LPVARRHGQRLPDYPRLGAEQFDLVFLDPPAWARSAFGTVDLLRDYQSLLKPALLATA
                     EGGVLVCCNNLAKVELDEWREQVLRCASKSGRPVREAEVLTPAADFPSHDGRHPLKTL
                     VLHL"
     gene            complement(2435..4372)
                     /gene="acs"
                     /locus_tag="VIO83_01370"
     CDS             complement(2435..4372)
                     /gene="acs"
                     /locus_tag="VIO83_01370"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY8329968.1"
                     /translation="MFDIRVHPVAAATYQHAFLHADDYRRLYRQSIEQPEIFWAEQAR
                     RFLDWFKPWRSVHHGDFAKGQASWFKGGQLNVAYNCLDRHLAQRGEQTAIIWEGDDPA
                     QSSRLTYRQLHEQVCRLANALKHLGVNKGDRVCIYLPMIPEAACAMLACARIGAVHSV
                     VFGGFAPDALRDRILDADCRLVITADESLRGGKHIALKANVDHALQDCPNVASVLVVA
                     HTHAGIDWFAGRDHWYHEALGQVDADCPAEPMDAEDPLFILYTSGSTGKPKGVMHSTA
                     GYLLATAMTFRYVFDYHDGEVFWCTADIGWVTGHSYGIYGPLANGATTLMYEGVPTYP
                     DAARPWQVVDKHQVNIFYTAPTALRALMREGEAPVQRTSRASLRLLGSVGEPINPEAW
                     EWYYHVVGDSRCPIVDTWWQTETGAIMISPLPGATPLKPGSATRPFFGVQPVLLDEQG
                     HEVEGPGAGMLALKASWPSQIRSVYGDHPRMLDTYFKPYPGYYFTGDGARRDEDGDYW
                     ITGRVDDVLNVSGHRLGSAEVESALVLHDAVAEAAVVGYPHDLKGQGVYAFVTLMNAA
                     QPSEALRQELADLVAGEIGGFARPERIQWAAGLPKTRSGKIMRRILRKIACNELDNLG
                     DLSTLADPSVVDALIEQRLQR"
     gene            complement(4483..6147)
                     /gene="pgi"
                     /locus_tag="VIO83_01375"
     CDS             complement(4483..6147)
                     /gene="pgi"
                     /locus_tag="VIO83_01375"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY8329969.1"
                     /translation="MEHYLKPLDATQQPAWQALRAQRELMQQFNLRQAFAADPQRFRR
                     YSLSQGGLFLDYSKNLIDERTLGLLVELAEQMKLSQAIGQLFSGAPVNASERRPALHT
                     ALRRPVGEQVLVDGVDVMPQVHRVLAQMTELVQRIHNGLWRGYTEKPITDVVNIGIGG
                     SFLGPQLVSEALLPFAQRGVRCHYLANIDGSEFHEVTANLRAETTLFIVSSKSFATLE
                     TLKNAQAARRWVLDQGCAVADLHRHFIAVSSNRQAAVEFGIAAENIFPMWDWVGGRYS
                     LWSAIGLPIALAIGMGNFKELLSGAYAMDQHFQSASFEQNMPVLLALLGVWYGDFWGA
                     HSHAILPYDHHLRNFNQHLQQLDMESNGKSVRQDGSPVSCGTGPVIWGGVGCNGQHAY
                     HQLLHQGTQLIPADFIVPVTSYNPLADHHQWLYANCLSQSQALMCGKTRAEAEAELRD
                     QGLSEDEVQRLAPHKVIPGNRPSNTIVLERVSPRRMGALVALYEHKVFVQSVIWGNNA
                     FDQWGVELGKELGKNLYGRLTGEDATPAADGSTQGLIDFFRARHRG"
     gene            complement(6230..6610)
                     /gene="panD"
                     /locus_tag="VIO83_01380"
     CDS             complement(6230..6610)
                     /gene="panD"
                     /locus_tag="VIO83_01380"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEY8329970.1"
                     /translation="MHSIMLKAKLHRAQVTHSVLDYEGSCAIDGEWLDLAGIREYEQI
                     QLYNVDNGERFTTYAIRAEDGSKTISVNGAAAHKADVGHRLIICTYAQYSEAELAQHK
                     PQMLYFNPGNQLSHTSQAIPVQVA"
     gene            complement(6624..7484)
                     /gene="panC"
                     /locus_tag="VIO83_01385"
     CDS             complement(6624..7484)
                     /gene="panC"
                     /locus_tag="VIO83_01385"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008015101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY8329971.1"
                     /translation="MITVKTVRELRAAVARARSEGKRIGLVPTMGNLHSGHVALVAKA
                     AQRADFVVASIFVNPLQFGAGEDLDNYPRTLAADQEKLLEAGCHLLFAPNVEEMYPDG
                     MAGQTIVSVPGVSEGLCGGSRPGHFDGVSTVVTKLFHMVQPDLAIFGEKDFQQLAVIR
                     KLVRDLDLPIQIIGEPIVRAADGLALSSRNGYLSEAQRASAPALYRVLSDLAAALRSG
                     RHDYPALLEGANARLSEAGFVPDYLEIRTPLTLQPATPADRQLVILAAARLGTTRLID
                     NLLVDLDATA"
     gene            complement(7481..8281)
                     /gene="panB"
                     /locus_tag="VIO83_01390"
     CDS             complement(7481..8281)
                     /gene="panB"
                     /locus_tag="VIO83_01390"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY8329972.1"
                     /translation="MPDVTLTTLQGYKQRGEKIAMLTCYDATFAKVACEAGVEMLLIG
                     DSLGMVLQGHDSTLPVSVADMAYHTACTKRGNRGALIVADMPFMANATLAQALDNAAT
                     LMRAGAHMVKLEGGAWLGETVRRLADNGIPACVHLGLTPQSVNVFGGYKVQGRGEAQA
                     RQLLADAVALEQAGAAMLLLECVPSTLAAEVTRAVGIPVIGIGAGAGTDGQVLVLHDA
                     LGLSLSGHVPKFVKNFMTGQSSIPDALAAYVRAVKDGQFPAPEHGFSA"
     gene            complement(8386..>8827)
                     /gene="folK"
                     /locus_tag="VIO83_01395"
     CDS             complement(8386..>8827)
                     /gene="folK"
                     /locus_tag="VIO83_01395"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY8329973.1"
                     /translation="AALGRLPRCRLVAQSSLYASDPLGPPDQPRYVNAVAALDCELAP
                     LELLDALQAIEREQGRVRKAERWGPRTLDLDILLFGNLQLDEERLRVPHYHMHARAFV
                     LYPLAEIAPQLVLPDGRTLQALLAACPPQGLERLPAAANPAVTT"
BASE COUNT         1290 a         2793 c         3313 g         1431 t
ORIGIN      
        1 acggccggct ggtccagcgt ttgggcgcgg ggggcacgat gctgctccag ggccgccaac
       61 tggcggtcca gcagggcctg ccagtcgagg cggccgtcgg cctcgcgcgc cgcgcgggct
      121 tccagcccct ggctgtgcag ctggccgatg accacctggc gctcggcgag gttgagggcg
      181 gtcccggtca ctgccaggct gtccagcttg agtggcggct gctcggcgag accgtgcagg
      241 gtgagcgtgg ccaggcgcag ttcgccgttg ctcagctgca gctgcgggcc ggggtggaac
      301 tccaggcgct cggcggtagc ctccagctgc tgcagatcga gcagtggccg gccatccgcc
      361 tcgtgcactt gcaggccatt cagctgcagc tggctgttgt ccagctgcag ctgcaggccg
      421 tccttcagct ccaggcggta gtcgctggcc aggctggcca cgccctgttc cagctccagc
      481 ggcaacagcc ggcgcacata gggccaccag ctcttcaggg cgatgtcggt gatgctcagg
      541 tggccttcgg cacgcagtgg ctgcaggctc agcagcgcct gcagcgacac gctggcgcca
      601 ctcgagccgc tggcggtgag acggagctgg ccctggtcct cggcgcgggt gccgaagtcg
      661 agcagctcga tggcgaggtt ctcgtagcgc acctcgacgc tcggttgcgg gcgcaggtca
      721 ttgaggtgca ggctgccgcc gctcagctgc aggcggtcga catgcagagg aaacagctcg
      781 ccggcgggct gcgcttcggc tgcgctcgct gccggcggca gcctgaacag ctgcagcagg
      841 ttgaacgtcc ggtccgtggc gaacagcagc tcgacatgcg gctgctccag ctgcagctcc
      901 agcaggtgca gcgcgccctg ccacaggctg tcgacctgca ggttggcgta caggcggctg
      961 aaggccagtt gctcggcacc gggggcaccg aggcgcagct ggtgcagggt cagttcgagg
     1021 ctgaagggat tgaactcgat gcgctcgagg ctggccggca gcgtggcgta ctcggccagc
     1081 ttctggttgg ccagcttcag gcccgcccag ggcagcagga agaagccgag cagggcatac
     1141 agcagcgggg cgagtaccaa ggcggcaagg ccgcgtttca atcctttgga catggctggg
     1201 cgcttcggtc aatgcatgaa gtgccttgga gtatggcatg cgctgccggt tccgaatgtg
     1261 gcgggctccg ccagttcggc aagggcggca ggcggcggga tagcgtcccg accgcgagtc
     1321 gggcgcgatc ccgcaggcta cggcgtcaga ggtgcaggac cagcgtcttc agcggatgac
     1381 ggccgtcgtg ggaggggaag tcggcggccg gggtcagcac ctcggcctcg cgcaccggtc
     1441 ggccgctctt gctggcgcag cgcagcacct gctcgcgcca ctcatccagt tcgaccttcg
     1501 ccaggttgtt gcagcacacc aggacaccgc cctcggcggt ggccagcagg gccggcttga
     1561 gcaggctctg gtagtcgcgc agcaggtcga cggtgccgaa ggcgctgcgc gcccaggccg
     1621 gcgggtcgag gaacaccaga tcgaactgct cggcgcccag ccgcgggtag tccggcaggc
     1681 gctggccgtg gcggcgggcc accggcaggc cggcgaactg gcggatggcg gggaagtagt
     1741 cggaggcgac gaagcgcatc gacgcaaggt ccggattgag cgcggcattc tcgcgaccga
     1801 ccgccaggtt gccctcggcg aagtccaggt tggtgacgct gcgcgcgccg ccggccgcgg
     1861 cgcacaggcc gacgccgcag gtgtaggcga acaggttgag caccgacttg ccggcgctgt
     1921 ggcgcttgac ccagccgcgg gcgttgcgca ggtcgaggaa cagcagcggg tcctggccgg
     1981 catggcgggc gcgcacgcgg tagttgaggc cccattcgtg gccgaccaga tcggccagtg
     2041 cctccggcgt cgcctggtac accgggtcgc ggcggtcgat gcgcgagtgg ggttgcgagc
     2101 ggtcgttgta caccggcagc agcggcgtgg ccagggcctg ctggcagctg tccagcagtt
     2161 gcagcagggc gtcgcgctcg agcggctggt ggaagctctg caccagcagc tgcgggccgt
     2221 agcggtcgac ggtcaggccc ggcgcgcctt cctggctgcc gtggaacagc cggtagcagt
     2281 cggtgccctg ggcgtgcagt tcggcgaaga gggcctggcg gcgctcgagg gcggcgcaca
     2341 gcgcctggtt caaggcggac atgcgcgtct cctcggaaga tggcgcggca gtttaccagc
     2401 cctgtcgcct cgacgcccgt gccgagcagg ccgctcagcg ctgcaggcgc tgctcgatca
     2461 gcgcgtccac caccgacgga tcggcgaggg tcgacaggtc gccgaggttg tccagctcgt
     2521 tgcaggcgat cttgcgcagg atgcgccgca tgatcttgcc cgagcgggtc ttcggcaggc
     2581 ccgctgccca ctggatccgc tcggggcggg caaagccgcc gatctcgccg gccaccaggt
     2641 cggccagctc ctggcgcagc gcctcgctgg gctgggcggc gttcatcagg gtgacgaagg
     2701 cgtacacgcc ctggcccttg aggtcgtggg gatagccgac caccgccgcc tcggccaccg
     2761 cgtcgtgcag caccagcgcg ctttccacct cggcgctgcc caggcggtgg ccggagacgt
     2821 tgagcacgtc gtcgacccgc ccggtgatcc agtagtcgcc gtcctcgtcg cggcgcgcgc
     2881 cgtcgccggt gaagtagtag ccgggataag gcttgaagta ggtgtcgagc atgcgcgggt
     2941 gatcgccgta gacgctgcgg atctgactcg gccagctggc cttgagcgcc agcatgccgg
     3001 cgccgggacc ctcgacctcg tgcccctgtt cgtccagcag caccggctgc acgccgaaga
     3061 acggccgggt tgccgagccg ggcttgaggg gggtggcgcc gggcagcggg ctgatcatga
     3121 tggcgccggt ctcggtctgc caccaggtgt cgacgatcgg acagcggctg tcgccgacca
     3181 cgtggtagta ccactcccag gcttccggat tgatcggctc gcccaccgag ccgagcaagc
     3241 gcaggctggc gcgcgaggtg cgctgcaccg gcgcttcgcc ttcgcgcatc agcgcgcgca
     3301 gggcggtggg ggcggtgtag aagatgttca cctggtgctt gtcgaccacc tgccaggggc
     3361 gcgcggcgtc cggataggtc ggcacgccct cgtacatcag ggtggtggcg ccgttggcca
     3421 gcgggccgta gatcccgtag ctgtggccgg tgacccagcc gatatcggcg gtgcaccaga
     3481 acacctcacc gtcgtggtag tcgaacacgt agcggaaggt catcgccgtg gccagcaggt
     3541 agccggcggt cgaatgcatc acccccttgg gtttgccggt ggagccggag gtgtagagga
     3601 tgaacagcgg gtcttcggcg tccatcggct cggccgggca gtcggcgtcg acctggccga
     3661 gcgcctcgtg gtaccagtgg tcgcggccgg cgaaccagtc gatgccggca tgggtatggg
     3721 cgaccaccag cacgctggcg acgttcgggc agtcctgcag ggcgtggtcg acattggcct
     3781 tgagggcgat gtgcttgccg ccgcgcaggc tctcgtctgc ggtgatcacc aggcggcagt
     3841 cggcatcgag gatgcggtcg cgcagggcat ccggggcgaa gccgccgaac accaccgagt
     3901 gcacggcacc gatgcgcgcg caggcgagca tggcgcaggc cgcctcggga atcatcggca
     3961 ggtagatgca tacccggtcg cccttattca cgcccaggtg cttgagcgca ttggccaggc
     4021 ggcagacctg ctcgtgcagt tggcgatagg tcaggcggct ggactgggcc ggatcgtcgc
     4081 cctcccagat gatcgccgtc tgttcgccgc gctgcgccag gtggcggtcg aggcagttgt
     4141 aggcgacgtt gagctggccg cccttgaacc agctggcctg gcccttggcg aagtcgccgt
     4201 ggtgcacgct gcgccagggc ttgaaccagt cgaggaagcg gcgcgcctgc tcggcccaga
     4261 agatctccgg ctgctcgatg gactggcggt acaggcgccg gtagtcgtcg gcatgcagga
     4321 aggcgtgctg gtaggtggcg gcagcgaccg ggtgaacacg gatgtcgaac atggcgcagc
     4381 tcctgggagg ctgtccgcga tgtccttcag cgtggacccg gagggagggg cttgcaagtc
     4441 acggcccgcc accgctcggg gcgggccgca gggggcgggg gatcagccgc gatggcgggc
     4501 gcggaagaag tcgatcagac cctgggtcga gccgtccgcg gccggcgtcg cgtcctcgcc
     4561 ggtgaggcgg ccgtagaggt tcttgcccag ctccttgccc agctcgacgc cccactggtc
     4621 gaaggcgttg ttgccccaga tcacgctctg cacgaacacc ttgtgttcgt agagggcgac
     4681 cagcgcgccc atgcgccgcg ggctgacgcg ttcgaggacg atggtgttgc tcggccggtt
     4741 gccggggatg accttgtgcg gcgccaggcg ctgcacttcg tcctcgctca ggccctggtc
     4801 gcgcagctcg gcctcggctt ccgcgcgggt cttgccgcac atcagtgcct ggctctgcga
     4861 caggcagttg gcgtacagcc actggtggtg gtcggccagc gggttgtagc tggtcaccgg
     4921 gacgatgaag tcggcgggaa tcagctgggt gccctggtgc agcagctggt ggtaggcgtg
     4981 ctggccgttg cagccgacgc cgccccagat caccggcccg gtgccgcagc tgaccgggct
     5041 gccgtcctgg cgcacgctct tgccgttgga ctccatgtcc agctgctgca ggtgctggtt
     5101 gaaattgcgc aggtggtggt cgtagggcag gatcgcgtgg ctgtgggcgc cccagaagtc
     5161 gccgtaccag acgccgagca gggccagcag caccggcatg ttctgctcga aggaggcgct
     5221 ctggaaatgc tggtccatgg cgtaggcgcc ggacagcagc tccttgaagt tgcccatgcc
     5281 gatggccagg gcgatcggca ggccgatcgc cgaccacagc gagtagcgcc cgccgaccca
     5341 gtcccacatc gggaagatgt tttccgcggc gatgccgaac tcgaccgcgg cctggcggtt
     5401 gctcgacacg gcgatgaagt ggcggtgcag gtccgccacg gcgcagccct ggtcgagcac
     5461 ccagcggcgc gcggcctggg cgttcttcag ggtctccagg gtggcgaagg atttcgacga
     5521 gacgatgaac agggtggttt ccgcgcgcag gttggcggtc acctcgtgga actcgctgcc
     5581 gtcgatgttg gccaggtagt ggcagcgtac gccgcgctgg gcgaacggca gcagggcctc
     5641 ggagaccagc tgcgggccga ggaaggagcc gccgatgccg atgttgacca cgtcggtgat
     5701 cggcttctcg gtgtagccgc gccacaggcc gttgtggatg cgttgtacca gctcggtcat
     5761 ctgggcgagg acgcggtgca cctgcggcat cacgtcgacg ccgtcgacca gcacctgctc
     5821 gccgaccggg cggcgcaggg ctgtgtgcag cgccgggcgg cgctcggagg cgttcaccgg
     5881 cgcgccagag aacagctgcc cgatcgcctg cgacagcttc atctgttcgg ccagctcgac
     5941 cagcaggccg agggtgcgct cgtcgatcag gtttttcgaa tagtcgagga acaggccacc
     6001 ctggctcagc gagtagcggc gaaagcgttg cgggtcggcg gcgaaggcct gacgcaggtt
     6061 gaactgctgc atgagctcgc gctgcgcgcg cagggcctgc cacgccggtt gctgggtggc
     6121 gtccagcggt ttgaggtagt gttccatcga ctgatccggt gtctggtgat ggctatagga
     6181 aaaagacaag cccggaaagt tccgggcttg tcgggcgacg catagcaggt caggcgacct
     6241 gcacgggaat cgcctggctg gtgtggctga gctggttgcc cgggttgaaa tacagcatct
     6301 gcggcttgtg ctgggccagt tcggcttcgc tgtactgggc gtaggtgcag atgatcaggc
     6361 ggtggccgac gtcggccttg tgcgcggcgg cgccgttcac cgagatggtc ttcgagccgt
     6421 cctcggcgcg gatcgcatag gtggtgaagc gctcgccgtt gtcgacgttg tacagctgga
     6481 tctgctcgta ttcgcggatg ccggccagat ccagccattc gccgtcgatg gcacaggagc
     6541 cttcgtagtc cagtacggaa tgggtcactt gggcgcggtg cagcttggct ttgagcatga
     6601 tgctatgcat ggcgggttcc tcttcaggcc gtggcgtcga gatccacgag cagattgtcg
     6661 atcagccgag tcgtgccaag gcgggctgcg gccaggatca ccaactggcg gtcggcaggg
     6721 gtggccggct gcagggtgag gggggtgcgg atttccaggt agtccggcac gaaaccggcc
     6781 tcgctcaggc gggcattggc cccctcgagc agggccgggt agtcgtgccg cccgctgcgc
     6841 agcgcggcgg ccaggtcgct cagcacccgg tacagcgcgg gggcgctggc gcgctgcgcc
     6901 tcgctgaggt agccgttgcg cgaggacagc gccaaaccgt cggcggcacg cacgatcggc
     6961 tcgccgatga tctggatcgg caggtcgaga tcgcggacca gcttgcggat caccgccagt
     7021 tgctggaagt ccttctcgcc gaagatcgcc agatcgggct ggaccatgtg gaacagcttg
     7081 gtcaccaccg tggacacgcc gtcgaagtgg ccggggcggc tgccgccgca gaggccttcg
     7141 gagacgcccg gcacgctgac gatggtctgc ccggccatgc cgtcgggata catttcctcg
     7201 acgttcggcg cgaacagcag gtgacagccg gcctcgagga gtttctcctg gtcggcggcg
     7261 agggtgcgcg gatagttgtc gagatcctcg ccggcgccga actgcagcgg gttgacgaag
     7321 atgctggcga ccacgaaatc ggcgcgctgg gcggccttgg ccaccagcgc gacatggccg
     7381 ctgtgcaggt tgcccatggt gggcaccagg ccgatgcgct tgccttcgct gcgtgcgcgg
     7441 gctacggcgg cgcgcagttc gcgaacggtc ttgacggtga tcatgcggag aaaccatgtt
     7501 cgggggcggg gaactggccg tccttgaccg cccgtacgta ggcagccagg gcatccggga
     7561 tgctgctctg gccagtcatg aagttcttga cgaatttggg cacgtggccg gacaggctca
     7621 ggccgagggc gtcgtgcagc accagtacct gaccgtcggt gccggcgccg gcaccgatgc
     7681 cgatcaccgg aatgccgacg gcgcgggtga cttccgcggc cagggtgctg ggcacgcact
     7741 ccagcagcag catggcggcg ccggcctgtt cgagggccac ggcatcggcg agcagttgcc
     7801 gggcctgggc ctcgccgcgg ccctggacct tgtagccgcc gaagacgttg accgactgcg
     7861 gggtgaggcc aaggtggacg caggccggaa tgccgttgtc ggccagccgg cgcacggttt
     7921 cgccgagcca ggcgccgcct tccagcttga ccatgtgcgc gccggcacgc atcagcgtgg
     7981 cggcgttgtc cagcgcctgc gcgagggtgg cattggccat gaacggcatg tcggcaacga
     8041 tcagcgcgcc ccggttgccg cgcttggtgc aggcggtgtg gtaggccatg tcggcgacgc
     8101 tgaccggcag ggtactgtcg tggccctgca ggaccatgcc cagcgagtcg ccgatcagca
     8161 gcatctcgac gccggcttcg caggctacct tggcgaaggt ggcgtcatag caggtgagca
     8221 tggcgatctt ctcgccacgc tgcttgtaac cctgcagggt ggtgagggtg acatcaggca
     8281 ttgtggttat cctcgttggc gccaacctgt accggcgcgg ggcaaacggt tgcttatagt
     8341 cctgatgggg ggggatgaag tcaattacgc ggtgttaccg gcgcgttacg tcgttaccgc
     8401 cgggttggcc gccgcgggca ggcgctccag cccctgcggc gggcaggcgg cgagcagtgc
     8461 ctgcagcgtg cggccgtccg gcagcaccag ctgcggggca atctccgcca gcgggtagag
     8521 cacgaaggcc cgcgcgtgca tatggtagtg cggcacgcgc aggcgctcct cgtccagctg
     8581 caggttgccg aacagcagga tgtccagatc gagggtgcgc ggcccccagc gctcggcctt
     8641 gcgcacgcgg ccctgctcgc gttcgatggc ctgcagcgca tcgagcagtt ccagcggcgc
     8701 cagctcgcag tccagcgcgg ccacggcgtt gacgtagcgc ggctggtcgg gcgggccgag
     8761 cgggtcgctg gcatacagtg aggactgcgc caccagccgg cagcgcggca ggcggccgag
     8821 ggcggcg
//
LOCUS       DATCUN010000041         5527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_20229, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000041 DATCUN010000000
VERSION     DATCUN010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20229"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1736
                     /locus_tag="VIO83_01400"
     CDS             75..1736
                     /locus_tag="VIO83_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HEY8329974.1"
                     /translation="MPKAARKDRRRAGKPPYLRELLAVAVALSSGAVSALPTVELGES
                     TTLESSLTVNYTASMRTEKADDEYLNDLNADDGTRNFDRGSLITNRVSLFGEALLRHD
                     NLGAMLRASHFYDAAYRSSNDNDSPLTVNKIGAHDHFTEKSRERSGGEFRLLDAFVFG
                     SWDLDGRYLSAKAGRHLVAWGESLFWPNISQGQAPVDATKFNVPGTEAKDAYLPVGQV
                     SASFTLTDELTLVGFWQYKWEETLLSPVGDYFGSDYFGPGAQFFRLAPGMISGLPDQS
                     FRVVNYAGEVEPDDDGQWGLGLRYQLAQNTEVGLYHYRYHDRVASLFFDFTGNTQYSS
                     LKSVGRGTGAGNAPAFQLGYFDDIALTGVSLSTKLGDAVQIGSDLSYREDAAVYLSNG
                     APTRGNLIQGNLNAVYMLGPSALAHQTTLMGEVMHQRIDSVDTLHVSGGMPGENGSFD
                     DFEYDGQTRGSTLVGVGAMFDYPSLFSGWDLTTKVYWNQNVSGSALSGLGRDEKRITV
                     GGDFKYLGNFQVGMTYVGYLGSADIANGRTLADRDYVSLNAKYTY"
     gene            1752..3128
                     /locus_tag="VIO83_01405"
     CDS             1752..3128
                     /locus_tag="VIO83_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HEY8329975.1"
                     /translation="MKKHTQLASLLACGALFALATPALAKTAEAAQLDSSTYTCTGAE
                     RAGNADGSIPEYSGKWLGAPPHVNFQGTGNHPLDPYADEKPLFTITAANMAEHEQHLT
                     DGMKALFRQYPDTFSMPVYPTHRDFRFNDSVCEATKANVGFARLTDDGEGVVAKTGGT
                     PFPMPKNGLELLKNSSLFTIRAWTEEYVSDNAYVLRDGKVNWGRVHSKNLAPHLEPGR
                     IGDTNGHSAFYLNQTLLPLRDKGEVNTGVEFWNDKTEPRQSWRYDPGTRRVRQAPGYG
                     FDMAFPGTGGSITVDEVRLFNGSGQRYDWKIVEKKELYIPYNAYRIHSPELKYADLLK
                     PGHIDPTHMRYEKHRVWVLEGTLKTDYRHLYGKRRLYIDEDTWFPIAADNYDSRGELW
                     RTSMLNFFYAYETQTMQAGVGLYHDLLENTYLAFNLINEQPKGFKLNVAGFRPAMFGP
                     EAARRGGL"
     gene            3128..4285
                     /locus_tag="VIO83_01410"
     CDS             3128..4285
                     /locus_tag="VIO83_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8329976.1"
                     /translation="MDGAGGACAPCLAAGTSYAELQGLIGLVYDGLLEDKPWNALLDA
                     LRRQFGANYASLTIRLPSAEDRGLVVFAGEARPHIHALYSSSLYAVDPFVNLPRDTVV
                     MLEELIDEREWLASVIYRDFLEPLQVRYMMGADIRSDDGREREFRLRLSRPPQSGPFS
                     ETERALCGLLLPHLKRALRLYAHLDRVESACRLYVGTLERMGIGSILLDEAGGICRIN
                     RAAEAILAEGDSLQLHNGMLEAVAPRDDRRLWRLIKQAAAGRNGSGLVEAIALDRGGQ
                     PGGLSVLVRSIPLAAEAGRAAAVELILRDSNRPAQPSEPLLRQLYQLTPAEASLAVLL
                     GEGLTLEESAARLGISRNTARSHLRAVFAKTGVKRQTALVQLLLCSVVAMD"
     gene            complement(4302..5432)
                     /locus_tag="VIO83_01415"
     CDS             complement(4302..5432)
                     /locus_tag="VIO83_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY8329977.1"
                     /translation="MEQPRWIWQQPDWPHFRWQAERLAPLLRDCTQAQGRLLGMAGAA
                     GGEFGAQSELDALLQNIVTSSAIEGEQLNVGSVRSSLARRLGLETEAQARVSPRSEGL
                     AELMLDATQQFELPLTPQRLLHWHALLFPEQDLDLLPRRIRVGALRGEEPMQVVSGRL
                     DRPTVHFEAPPREGLEEQLAAFLDWFTASRNDATLDPLLRAGIAHFWFVTLHPFDDGN
                     GRLTRAITDLALAQGERQAIRFYAMSASILDDRRGYYRILESSQKAGLDITDWLEWFL
                     HTLLRSLQQALARIDRVLAKSRFWQQHRGQPLSAEQIKVLNRLLDGGERGFEDGISAA
                     QYQAVAKVSKATATRHLADLLDRGCLCKLPGGGRSTRYRIAE"
BASE COUNT          953 a         1896 c         1853 g          825 t
ORIGIN      
        1 atggacctgc ccgcgtggtg gcgttgcccg agcgcgggcc tgcaatgcca gaacaacaat
       61 aatccggagg cagtatgccc aaggcagcaa gaaaggatcg ccggcgcgcc ggcaagccac
      121 cgtacctgcg cgagctgctc gccgtggccg tggcactgag cagcggggcg gtgagcgccc
      181 tgccgaccgt cgagctgggc gagtcgacca ccctcgaatc gtcgctgacg gtcaactaca
      241 ccgcctcgat gcgcacagag aaggccgacg acgagtatct caacgatctc aacgccgacg
      301 acggcacgcg caacttcgac cgcggctcgc tgatcaccaa ccgggtcagc ctgttcggcg
      361 aggcgctgct gcgccatgac aacctcggtg cgatgctgcg cgccagccac ttctacgatg
      421 cggcctaccg gagcagcaac gacaacgact cgccgctgac cgtcaacaag atcggcgccc
      481 acgaccattt caccgagaag agccgcgagc gcagcggcgg cgaattccgt ctgctggacg
      541 ccttcgtgtt cggcagctgg gacctcgacg gccgctacct gtcggccaag gctggccgcc
      601 acctggtggc ctggggcgag agcctgttct ggccgaacat cagccagggc caggcgccgg
      661 tggacgccac caagttcaac gtgccgggca ccgaggccaa ggacgcttac ctgccggtcg
      721 gccaggtgtc ggcgtccttc acgctgaccg acgagctgac cctagttggc ttctggcagt
      781 acaagtggga agaaaccctg ctcagcccgg tgggcgacta cttcggcagc gactacttcg
      841 ggcccggcgc gcagttcttc cgcctggcgc cagggatgat aagcgggttg ccggaccaga
      901 gcttccgggt ggtcaactac gccggcgaag tggagccgga cgacgacggc cagtggggcc
      961 tgggcttgcg ctaccagctg gcccagaaca ccgaggtcgg cctctaccac taccgctacc
     1021 acgaccgggt ggcgtcgctg ttcttcgact tcaccggcaa cacccagtac tcctcgctca
     1081 agagcgtcgg ccgcggtacc ggggcgggca acgcgccggc cttccagctc ggctatttcg
     1141 acgacatcgc cctcaccggg gtgagcctga gcaccaagct cggcgatgcg gtgcagatcg
     1201 gcagcgacct cagttaccgc gaggacgccg cggtgtacct gtccaacggc gcaccgaccc
     1261 gcggcaacct gatccagggc aacctcaacg ccgtctacat gctcggcccg agcgcgctgg
     1321 cccaccagac cacgctgatg ggcgaggtga tgcaccagcg catcgacagc gtcgacaccc
     1381 tgcacgtcag cggcggcatg ccgggcgaga acggcagctt cgacgacttc gagtacgacg
     1441 gccagacccg cggcagcacg ctggtcggtg tcggcgcgat gttcgactac ccgagcctgt
     1501 tcagcggctg ggacctgacc accaaggtgt actggaacca gaacgtctcc ggcagcgctt
     1561 tgtccggcct gggccgcgac gagaagcgca tcaccgtcgg cggcgacttc aagtacctgg
     1621 gcaacttcca ggtcggcatg acctacgtcg gctatcttgg ctcggccgac atcgccaacg
     1681 gccgcaccct ggcggatcgc gactacgtgt cgctgaacgc caagtacacc tactaagaag
     1741 gggagggacc catgaagaag cacacgcaac tcgccagcct gctcgcctgc ggcgcgctgt
     1801 tcgcgctcgc cacgccggcg ctggccaaga ccgctgaagc ggcccagctg gacagcagca
     1861 cctacacctg cacgggcgcc gagcgcgccg gcaacgccga cggcagcatt ccggagtatt
     1921 ccggcaagtg gctgggcgcg ccgccgcacg tgaacttcca gggcaccggc aaccatccgc
     1981 tcgatcccta tgcggacgag aagccgctgt tcaccatcac tgcggcgaac atggccgagc
     2041 acgagcagca cctcaccgac ggcatgaagg cgctgttccg gcagtatccc gataccttca
     2101 gcatgccggt ctatccgacg catcgcgatt tccgcttcaa cgacagcgtg tgcgaggcga
     2161 ccaaggccaa cgtcggcttc gcccggctga ccgatgacgg cgagggggtg gtggccaaga
     2221 ccggcggtac gccgttcccg atgccgaaga atggtctgga gctgctgaag aactcctcgc
     2281 tgttcaccat acgcgcgtgg accgaggaat acgtctcgga caacgcctac gtgctgcgcg
     2341 acggcaaggt caactggggt cgcgtgcact cgaagaacct ggcgccgcac ctggagccgg
     2401 gccgcatcgg cgacaccaac ggccactcgg ccttctacct caaccagacc ctgctgccgc
     2461 tgcgcgacaa gggcgaggtg aacaccggcg tggagttctg gaacgacaag accgagccgc
     2521 ggcagagctg gcgctacgac ccgggcaccc gccgcgtgcg ccaggccccc ggctatggct
     2581 tcgacatggc gttccccggc accggcggct cgatcaccgt ggacgaggtg cgcctgttca
     2641 acggttccgg ccagcgctac gactggaaga tcgtcgaaaa gaaggagctg tacatcccct
     2701 acaacgccta tcgcatccac tcgcctgagt tgaagtacgc cgacctgctc aagccgggcc
     2761 atatcgaccc gacccacatg cgctacgaga agcaccgcgt gtgggtgctg gaaggcaccc
     2821 tcaagaccga ctaccgccac ctgtacggca agcgtcgtct gtacatcgac gaggacacct
     2881 ggttccccat cgccgccgac aactacgaca gccgcggcga gctgtggcgc acctcgatgc
     2941 tcaacttctt ctacgcctac gagacccaga cgatgcaggc cggggtcggc ctgtaccacg
     3001 atctgctgga aaacacctac ctggcgttca acctgatcaa cgagcagccc aagggcttca
     3061 agctgaacgt ggcgggcttc cgcccggcga tgttcggacc ggaggcggca cgccggggcg
     3121 gtctgtgatg gacggggcag ggggcgcgtg cgccccttgc ctcgctgcag gcaccagcta
     3181 cgccgagctg caggggctga tcggcctggt ctacgacggc ctgctcgagg acaagccgtg
     3241 gaacgccctg ctcgacgccc tgcgccggca gttcggggcc aactacgcct cgctgaccat
     3301 ccgcctgccg agtgccgagg atcgcggtct ggtggtgttc gccggcgagg cgcggccgca
     3361 catccatgcg ctgtacagca gctcgctgta cgcggtcgat ccgttcgtca acctgccgcg
     3421 cgacaccgtg gtgatgctcg aggagttgat cgacgagcgc gagtggctgg ccagcgtgat
     3481 ctaccgcgat tttctcgaac cgctgcaggt gcgctacatg atgggcgccg acatccgcag
     3541 tgacgacggc cgcgagcgcg aattccgcct gcgcctgagc cgtccgccgc agagcgggcc
     3601 attcagcgag accgagcggg cgctgtgcgg cctgctgctg ccgcacctca agcgcgcgct
     3661 gcgcctgtac gcccacctcg accgggtgga gagcgcctgc cggctgtacg tcggcaccct
     3721 ggagcgcatg ggcatcggct cgatcctgct cgacgaggcc ggtggaatct gccggatcaa
     3781 ccgggccgcc gaggccatcc tcgccgaagg cgacagcctg caattgcaca acggcatgct
     3841 cgaagccgtc gccccgcgcg acgaccgccg cctgtggcgg ctgatcaagc aggccgccgc
     3901 cggacgcaac ggcagcggcc tggtggaagc catcgccctc gaccgcggcg gccagcctgg
     3961 cgggctgagc gtgctggtgc gcagcatccc gctggccgcc gaagccggcc gcgccgccgc
     4021 cgtcgaactg atcctgcgcg acagcaaccg cccggcgcag ccctccgaac cgctgctgcg
     4081 tcagctctac cagctcaccc cggccgaggc ctcgctggcc gtgctgctcg gcgaaggcct
     4141 gaccctcgag gaaagcgccg cgcgcctggg catcagccgc aacacggcgc gcagccacct
     4201 gcgcgcggtg ttcgccaaga ccggcgtgaa gcggcagacc gcgctggtgc agctgttgct
     4261 gtgcagcgtg gtggcgatgg attgagtcgc gaccggcagg gctattccgc aatccggtaa
     4321 cgggtgctgc gcccgccacc gggcagcttg cacaggcagc ccctgtcgag caggtcggcc
     4381 agatggcggg tggcggtggc cttggagacc ttggcgacag cctggtactg agcagcgctg
     4441 atgccgtcct cgaagccgcg ttcgccgccg tcgagtaggc ggttgagcac cttgatctgt
     4501 tcggcggaca gcggttgacc ccggtgctgc tgccagaagc gcgacttggc cagcacgcgg
     4561 tcgatgcgcg ccagagcctg ctgcaggctg cgcagcaggg tgtgcaggaa ccattcgagc
     4621 cagtcggtga tgtccagccc ggccttctgg ctgctctcca ggatgcggta gtagccgcga
     4681 cggtcgtcga ggatgctcgc cgacatggcg tagaagcgga tcgcctggcg ttcgccttgg
     4741 gccaaggcca ggtcggtgat ggcgcgggtc aggcggccgt taccgtcgtc gaaggggtgc
     4801 agggtgacga accagaagtg cgcgatgccg gcgcgcagca gtgggtcgag ggtggcatcg
     4861 ttgcggctgg cggtgaacca gtcgagaaag gccgccaact gctcctccag gccctcgcgc
     4921 ggcggtgcct cgaaatgcac ggtgggacgg tcgaggcgcc cggacaccac ctgcatcggc
     4981 tcctcgccgc gcagggcgcc gacgcggatg cgccgtggca gcaggtcgag atcctgctcg
     5041 gggaacagca gtgcgtgcca gtgcagcagg cgctgcggcg tcagcggcag ctcgaactgc
     5101 tgggtggcgt ccagcatcag ctcggccagc ccctcgctgc gcggactgac gcgggcctgt
     5161 gcctcggtct ccaggcccag gcggcgggcc agcgaggaac gcaccgaacc gacgttgagc
     5221 tgttcaccct cgatggccga ggaggtgacg atgttctgta gcagggcatc cagctcgctc
     5281 tgcgcgccga actcgccacc ggcggcgccg gccatgccga gcagccggcc ctgggcctgc
     5341 gtgcagtcgc gcaacagagg cgccagacgc tcggcctgcc agcggaaatg cggccagtcg
     5401 ggctgctgcc agatccagcg gggttgttcc atcgtctgcc tgctcgcggg tggtgagccg
     5461 attatgggat ttattcggct catttggtga gccgattatg gcggctattc ggctcagggg
     5521 ccagtcg
//
LOCUS       DATCUN010000042         9132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_114366, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000042 DATCUN010000000
VERSION     DATCUN010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114366"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2278
                     /gene="lon"
                     /locus_tag="VIO83_01420"
     CDS             <1..2278
                     /gene="lon"
                     /locus_tag="VIO83_01420"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY8329978.1"
                     /translation="FFPAQVLPIMVNPQHWAETLQRVAKTEHHCVALFYMDNPPDSAE
                     DFDPDSLPTHGTVVRVHHASKQDDTLQFVAQGLARVRIRNWLSRTPPYLVEVDYPRTP
                     ADPSDEVKAYGMALINAIKELLPLNPLYSEELKNYLNRFSPNDPSPLTDFAAALTTAP
                     GSDLQAVLDTVPVLKRMEKVLPLLRREVEVAKLQNEISAEVNSTIGKRQREFFLREQL
                     KVIQKELGITKDDKSADSEEFRKRLQGKTLPAAAQKRIDEELNKLSVLETGSPEYAVT
                     RNYLDWATSVPWGVLGKDKLDLKHARKVLDKHHAGMDDIKERIIEFLAVGAFKGEIAG
                     SIVLLVGPPGVGKTSIGKSIAESLGRPFYRFSVGGMRDEAEIKGHRRTYIGALPGKLV
                     QALKEVEVMNPVIMLDEIDKMGSSYQGDPASALLETLDPEQNVEFLDHYLDLRLDLSK
                     VLFVCTANTLDSIPGPLLDRMEVIRLSGYIGEEKLAIAKRHLWPRQLDKAGVPRQRLS
                     ISDAALRLVIEGYAREAGVRQLEKQLGKIVRKAVVKLLEDPAATLKVGPKELEDYLGM
                     PVFRKEQHLEGVGIITGLAWTSMGGATLPIEATRIHTLNRGFKLTGKLGEVMKESAEI
                     AYSYISSHLKKYKGDPTFFDQAFVHLHVPEGATPKDGPSAGISMASALLSLARNQAPK
                     TDLAMTGELTLTGQVLAIGGVREKVIAARRQKIFELILPEANRGDFEELPDYLKEGLT
                     VHFARRYADVAKVLFPAT"
     gene            2386..2793
                     /locus_tag="VIO83_01425"
     CDS             2386..2793
                     /locus_tag="VIO83_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HEY8329979.1"
                     /translation="MPILPALRLLPLAGLILLTACAQKAPSTLSLDEQPRYCDPLELH
                     EGQEFNLRLPSNPTTGFRWVMRENAAPQLKQLGPEVYTTPEEAGVVGSAGVSTWRFRA
                     VARGEARLELDYRRPWENGVAAAKNFDCRISVK"
     gene            2907..3476
                     /locus_tag="VIO83_01430"
     CDS             2907..3476
                     /locus_tag="VIO83_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017148769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329980.1"
                     /translation="MNKYGRQLLLSMTALLSACGSLLPSEQATVSSVFRDYGDAESRY
                     SQAVPGVTNRPELFSLGFDPLANGNGRLLSFLDVRTMFIQPNIPIDYLPDSLVTCLQA
                     KERCTGYAFEFTQTDTQRVGSFWADVLNFRKNREMKGWTFRAVFVLVDDKLVHKVSNG
                     EPQIRRMQVERNPLGPLQGAGEYLSDQLK"
     gene            3510..4286
                     /gene="cmoA"
                     /locus_tag="VIO83_01435"
     CDS             3510..4286
                     /gene="cmoA"
                     /locus_tag="VIO83_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008016734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HEY8329981.1"
                     /translation="MPGFSMPEARAVKQTPDRIYATPQEQVADFVFNEAVVRVFPDMI
                     KRSVPGYPAIVENIGVIASQFAQPHSTLYDLGCSLGAVSQALRRHVQAEDCRVIAVDN
                     SNAMVERCREYLHAQDSMFQELLPVEVLEADICQLEFRPASVVALNFTLQFIPREQRL
                     ELLQRIRQALRPGGALILSEKLRFEDAEEQELLTDLHIAFKRANGYSELEIAQKRSAI
                     ENVMKPDSLAEHRERLLAAGFSKVIPWFQCLNFASLIALP"
     gene            4283..5260
                     /gene="cmoB"
                     /locus_tag="VIO83_01440"
     CDS             4283..5260
                     /gene="cmoB"
                     /locus_tag="VIO83_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HEY8329982.1"
                     /translation="MTDLATRANLNALIDTLRGTPLAAWAASLPALLAGKLADGHGDL
                     PRWQAAVDALPPLTAQQRELAECFALAGACTAEQRTALEQALKGLIPWRKGPFELFGV
                     HVDTEWHSDWKWARVAPHLDLAGKRVLDVGCGNGYYQWRMLGAGADCVVGVDPNWLFF
                     CQFLAMKNYLADLPAWHLPLTLEELPEKLEGFDTVFSMGVLYHRRSPIDHLLALKDCL
                     VRGGELVLETLVVEGDVNTVLVPEDRYAQMRNVWFLPSVAALELWLRRAGFVDVRCVD
                     VSVTSIEEQRSTEWMKFQSLPEFLDPQDQRRTVEGLPAPMRAVVLARKP"
     gene            complement(5477..6220)
                     /gene="pdxJ"
                     /locus_tag="VIO83_01445"
     CDS             complement(5477..6220)
                     /gene="pdxJ"
                     /locus_tag="VIO83_01445"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY8329983.1"
                     /translation="MTEANRILLGVNIDHIATLRQARGTRYPDPVKAALDAEEAGADG
                     ITVHLREDRRHIQDRDVRVLREVLQTRMNFEMGVTEEMLAFAEEIRPAHACFVPETRQ
                     ELTTEGGLDVAGQEARIRAAVERLAAIGCKVSLFIDADPRQIEAAARIGAPAIELHTG
                     RYADAHTPAETADELARIRDGVECGLRHGLIVNAGHGLHYHNAEAVAAIQGINELNIG
                     HAIVAHALFVGFKQAVAEMKQLIVAAAAR"
     gene            complement(6213..6911)
                     /gene="recO"
                     /locus_tag="VIO83_01450"
     CDS             complement(6213..6911)
                     /gene="recO"
                     /locus_tag="VIO83_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY8329984.1"
                     /translation="MIAAAHPAFVLHSRPYKETSALVDFLTPEGRLRAVLRGARGKAG
                     TLARPFVPLELELRGRGELPNVARLESAGIPHLLSGHALFSGLYLNELLVRLLPAQDP
                     HPALFDHYAATLAALAGGRPLEPLLRAFEWRLLDDLGYGFALDRDCQGQPVDPATLYQ
                     WLPELGFQPVAQLQPGLFHGDELLALAEANWAQPGALSAAKRLMRQALAPHLGGRPLV
                     SRELFINRKESRRD"
     gene            complement(7006..7908)
                     /gene="era"
                     /locus_tag="VIO83_01455"
     CDS             complement(7006..7908)
                     /gene="era"
                     /locus_tag="VIO83_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011535347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY8329985.1"
                     /translation="MTESLPSRCGYVAIVGRPNVGKSTLLNHILGQKLAITSRKPQTT
                     RHTLLGIKTEGPVQAIYVDTPGMHKDNDKALNRYMNRSASAALKDVDVVIFVVDRTRW
                     TEEDQMVLDRVKYVEGPLLVVVNKIDRLEDKGELLPHLKWLAEQLPNAEIVPISAQHG
                     QNLETLEAQVAKHLPEGEHFFEEDQLTDRSSRFLAAELVREKVMRQLGAEVPYQVAVE
                     IEQFQQQGRVLHIHALILVERDGQKKIIIGDKGERIKRIGQEARRDMEELFGSKVMLN
                     LWVKVKGGWSDDERALRSLGYGDI"
     gene            complement(7901..8590)
                     /gene="rnc"
                     /locus_tag="VIO83_01460"
     CDS             complement(7901..8590)
                     /gene="rnc"
                     /locus_tag="VIO83_01460"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY8329986.1"
                     /translation="MSNPLQRLERRLGYKFQDPELMVLALTHRSFAGRNNERLEFLGD
                     AILNFVVGEALFKRFPQAREGQLSRLRARLVKGETLARLALVFDLGEYLRLGSGELKS
                     GGFRRESILADATEALIGAIYLDTGMEAARDRVLAWLAPYFDELTLVDTNKDPKTRLQ
                     EFLQSRGCELPRYDVVDIQGEPHCRTFFVECEIALLNQKTRGQGASRRIAEQVAAAAA
                     LVALGVENGND"
     gene            complement(8587..8961)
                     /locus_tag="VIO83_01465"
     CDS             complement(8587..8961)
                     /locus_tag="VIO83_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HEY8329987.1"
                     /translation="MMFARSQKGLSMLSWLVVLCIVGFIASVFLKVFPHYMDFYSLQK
                     IITSAATESAHQIRSPNDFYAHMGRGMEVNNLDFDLKEMMEVSMENDEFYVHLNYEKR
                     EPLIENLDLVVHFDREYRVRAP"
     gene            complement(8993..>9132)
                     /locus_tag="VIO83_01470"
     CDS             complement(8993..>9132)
                     /locus_tag="VIO83_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY8329988.1"
                     /translation="PAIPLALHGMVPDRNIVGKAFAVWMSWPEPKLSHLPTFSRVGLI
                     H"
BASE COUNT         1600 a         3083 c         2960 g         1489 t
ORIGIN      
        1 gttcttcccg gcccaggtgc tgccgatcat ggtcaacccg cagcactggg cggaaaccct
       61 gcagcgcgtg gccaagaccg agcaccactg cgtggcgctg ttctacatgg acaatccgcc
      121 ggacagcgcc gaagacttcg acccggacag cctgcccacc catggcaccg tggtgcgcgt
      181 gcaccacgcc agcaagcagg acgacaccct gcagttcgtc gctcagggcc tggcgcgggt
      241 gcgcatccgc aactggctga gccgcacgcc gccctatctg gtggaggtcg actacccgcg
      301 cacgccggcc gatcccagcg acgaggtcaa ggcctacggc atggcgctga tcaatgcgat
      361 caaggagctg ctgccgctca acccgctgta cagcgaggag ctgaagaact acctcaaccg
      421 cttcagcccc aacgacccct cgccgctgac cgacttcgcc gccgctctca ctaccgcgcc
      481 gggcagcgac ctgcaggcag tgctcgacac cgtgccggta ctcaaacgca tggagaaggt
      541 gctgccgctg ctgcgccgcg aggtggaggt ggcgaagctg cagaacgaga tcagcgccga
      601 ggtcaacagc accatcggca agcgtcagcg cgagttcttc ctgcgcgagc agctcaaggt
      661 gatccagaag gagctgggca tcaccaagga cgacaagagc gccgacagcg aggagttccg
      721 caagcgcctg caaggcaaga cgctgccggc ggcggcgcag aagcgcatcg acgaggagct
      781 gaacaagctg tcggtcctgg agaccggctc gccggaatac gccgtcaccc gcaactacct
      841 ggactgggcc accagcgtgc cctggggcgt gctgggcaag gacaagctcg acctcaagca
      901 cgcgcgcaag gtgctcgaca agcaccacgc cggcatggac gacatcaagg aacgcatcat
      961 cgagttcctc gccgtcggcg ccttcaaggg cgagatcgcc ggctccatcg tgctgctggt
     1021 cggcccgccc ggggtgggca agaccagcat cggcaagtcg atcgccgagt cgctgggccg
     1081 gccgttctac cgcttcagcg tcggcggcat gcgcgacgag gcggagatca agggccaccg
     1141 ccgcacctac atcggtgccc tgccgggcaa gctggtgcag gcactgaagg aggtcgaggt
     1201 gatgaacccg gtgatcatgc tcgacgagat cgacaagatg ggcagcagct accagggcga
     1261 cccggcctcg gcgctgctgg aaaccctcga cccggagcag aacgtcgaat tcctcgacca
     1321 ctacctcgac ctgcgcctgg acctgtccaa ggtgctgttc gtgtgcactg ccaataccct
     1381 cgactcgatc cccggcccgc tgctcgaccg catggaggtg atccgcctgt ccggctacat
     1441 cggcgaggag aagctggcca tcgccaagcg ccacctgtgg ccgcgccagc tggacaaggc
     1501 cggcgtgccc cggcagcgcc tgagcatcag cgatgcggca ctgcgcctgg tgatcgaggg
     1561 ctatgcccgc gaggcgggtg tgcgccagct ggagaaacag ctgggcaaga tcgtgcgcaa
     1621 ggcggtggtc aaactgctgg aagaccccgc agcgacgctg aaggtcgggc cgaaggagct
     1681 cgaggactac ctgggcatgc cggtgttccg caaggaacag catctggagg gtgtcggcat
     1741 catcaccggc ctggcctgga ccagcatggg cggcgccacc ctgccgatcg aggcgacgcg
     1801 catccatacc ctcaaccgcg gcttcaagct gaccggcaag ctcggcgagg tgatgaagga
     1861 gtcggcggag atcgcctaca gctacatcag ctcgcacctg aagaagtaca agggcgaccc
     1921 gaccttcttc gaccaggcct tcgtccacct gcacgtgccg gagggcgcca cgcccaagga
     1981 cggccccagc gccggcatca gcatggccag tgccctgctc tcgctggcgc gcaaccaggc
     2041 gcccaagacc gacctggcca tgaccggcga gctgaccctc accggccagg tgctggccat
     2101 cggcggggtg cgcgagaagg tgatcgccgc gcggcggcag aagatcttcg agctgatcct
     2161 cccggaggcc aaccgcggcg acttcgagga gctgccggac tacctgaagg aaggcctgac
     2221 cgtgcacttc gcccgtcgct acgccgacgt ggccaaggtg ctgttccccg cgacgtgagc
     2281 ggcagagcct ggactgcgga cgccaccgca gcccaggctc ggaaaacgtc atcgcaatcg
     2341 gctaggctgg cgccttgtca tcctgccact ttccggagcc cgccgatgcc catcctgcct
     2401 gccctgcgcc tgctgccgct cgccggcctg atcctgctca ctgcctgcgc gcagaaggca
     2461 ccgagcaccc tgagcctgga cgagcaacca cgctattgcg atccgctgga gctgcacgaa
     2521 ggccaggagt tcaacctgcg cctgcccagc aatcccacca ccggctttcg ctgggtcatg
     2581 cgcgaaaacg ctgcgccgca gctgaaacag ctcggcccgg aggtctatac gaccccggag
     2641 gaagccggcg tggtcggcag cgccggcgtc tcgacctggc gcttccgcgc ggtcgcccgt
     2701 ggcgaagcgc gcctggagct ggactatcgt cgcccctggg aaaacggcgt ggctgccgcc
     2761 aagaacttcg actgccgtat ctcggtcaag tagtcccgcg ccccccggca tccccagctc
     2821 accattcgtc tccagagcgg gcaggccatc gccaaatcgc ctatagcaat agtaacggct
     2881 gtgtgtcgtg gggggggatg gacatcatga acaagtatgg gcggcaactg ttgctcagta
     2941 tgacggcgct gctttccgcc tgcggcagcc tgctccccag cgagcaagca accgtgagtt
     3001 cggtgttccg cgactatggg gatgccgaaa gccgctactc gcaggccgtc cccggggtca
     3061 ccaaccgccc cgagctgttt tctctgggct tcgaccccct ggccaatggc aacggtcgcc
     3121 tgctgtcgtt tctcgacgtg cgcaccatgt tcatccagcc gaacatcccc atcgactacc
     3181 tgccggacag tctggtgacc tgcctgcaag ccaaggagcg ctgcaccggc tatgccttcg
     3241 agttcaccca gactgacacc cagcgggtcg gcagtttctg ggccgacgtg ctgaacttcc
     3301 gcaagaaccg cgagatgaag ggctggacgt tccgcgcggt attcgtgctg gtggacgaca
     3361 agctggtgca caaggtcagc aacggcgagc cgcagatccg ccgcatgcag gtcgaacgca
     3421 acccgctagg cccgctgcag ggcgccggcg agtacctttc cgaccagctg aagtgaaggt
     3481 gctgcgaggc agcccggcag tccgctagaa tgccgggctt ttccatgccg gaagcgcgcg
     3541 ccgtgaagca gacccccgac cgcatctacg ccactcccca ggagcaggtc gccgacttcg
     3601 tgttcaacga ggcggtggtg cgggtgttcc ccgacatgat caagcgctcg gtgccgggct
     3661 atcccgccat cgtcgagaac atcggcgtga tcgccagcca gtttgcccag ccgcacagca
     3721 cgctgtacga cctcggctgc tcgctgggtg cggtcagcca ggcgctgcgc cgccatgtgc
     3781 aggccgagga ctgccgggtg atcgcggtgg acaactccaa cgccatggtc gagcgctgcc
     3841 gcgagtacct gcatgcccag gactcgatgt tccaggagct gttgccggtc gaggtcctcg
     3901 aggccgacat ctgccagctc gagttccggc cggcctcggt ggtagccttg aacttcaccc
     3961 tgcagttcat cccgcgcgag cagcgtctcg aactgctgca acgcatccgc caggcgctgc
     4021 ggcccggtgg tgcactgatc ctctcggaga agctgcgctt cgaagatgcc gaggagcagg
     4081 aactgctcac cgacctgcac atcgccttca agcgggccaa cggctacagc gaactggaaa
     4141 tcgcccagaa gcgcagcgcc atcgagaatg tgatgaaacc ggacagcctc gccgaacacc
     4201 gcgagcgcct gctcgccgcc ggcttctcga aagtcatccc ctggttccaa tgcctgaact
     4261 tcgcctcgct gatcgccctg ccatgaccga cctcgccacc cgcgccaacc tcaacgccct
     4321 gatcgacaca ctgcgcggca cgccgctggc tgcctgggcc gccagcctgc ccgccctgct
     4381 cgccggcaag ctggccgacg gccacggcga cctgccgcgc tggcaggctg cggtcgatgc
     4441 tctgccgccg ctgactgcgc agcagcgcga gctggccgaa tgctttgccc tcgccggcgc
     4501 ctgcacggcc gagcaacgca cggcgctgga acaggcgctc aaggggctga tcccctggcg
     4561 caaggggccg ttcgagctgt tcggcgtgca cgtcgacacc gaatggcact cggactggaa
     4621 atgggcgcgg gtcgcccccc atctggacct ggccggcaag cgcgtgctgg atgtcggctg
     4681 cggcaacggc tactaccagt ggcgcatgct cggcgccggc gccgactgcg tggtcggcgt
     4741 cgaccccaac tggctgttct tctgccagtt cctcgccatg aagaactacc tggcggatct
     4801 gcctgcctgg cacctgccgc tgactctgga ggagctgccg gaaaagctcg agggcttcga
     4861 cacggtgttt tccatgggcg tgctctatca ccgccgctcg cccatcgacc acctgctggc
     4921 actgaaggac tgcctggtca ggggcggcga actggtcctg gaaaccctgg tggtggaggg
     4981 cgacgtcaac accgtgctgg tgccggaaga ccgctatgcg cagatgcgca acgtctggtt
     5041 cctgccctcg gtggcggcgc tggagctatg gctgcgccgc gccggcttcg tcgacgtgcg
     5101 ctgtgtggac gtcagcgtca ccagcatcga ggagcagcgc tccaccgagt ggatgaagtt
     5161 ccagtcgctg ccggaattcc tcgacccgca ggaccagcgc cgcacggtgg aaggcctgcc
     5221 ggcgccgatg cgcgccgtgg tgctggcgcg caagccgtaa tcatggtgcg cacggcgcac
     5281 cctacgagca ccactgcatt gatggttctc acgctcccgc gtgggaaccc gagcctgggc
     5341 gcagagcgcc cccggatgcg tgcccacgca ggagcgtggg cacgatcatg gatcttctac
     5401 cgactcctca gcggcagcct gcccggtagg caatcgctgc gcgagttgcc taccctacat
     5461 ctcgaccgcc tgcggctcag cgcgccgccg cggcgacgat cagctgtttc atctccgcca
     5521 ccgcctgctt gaagccgacg aacagcgcgt gggcgacgat ggcgtggccg atgttcagct
     5581 cgttgatgcc ctggatggcc gccaccgctt cggcattgtg gtagtgcagg ccatgaccgg
     5641 cattgacaat caggccgtgg cgcaggccgc actcgacgcc gtcgcggatg cgcgccagtt
     5701 cgtcggcggt ttccgccggg gtgtgcgcgt cggcataacg accggtgtgc agctcgatgg
     5761 ccggcgcacc gatgcgcgcg gcggcctcga tctgccgcgg atcggcgtcg atgaacagcg
     5821 acaccttgca gccgatggcc gccagtcgct cgaccgcggc gcgaatgcgc gcctcctgcc
     5881 cggccacgtc gagaccacct tcggtggtca gttcctggcg ggtctccggc acgaagcagg
     5941 cgtgcgccgg acggatctct tcggcgaagg cgagcatctc ctcggtgacg cccatctcga
     6001 agttcatccg cgtctgcagc acctcgcgca gtacgcgcac gtcgcggtcc tggatatgtc
     6061 ggcgatcctc gcgcaggtgc acggtgatgc cgtcggcgcc ggcttcctcg gcgtccagcg
     6121 cggccttcac cgggtcggga tagcgggtgc cgcgggcctg gcgcagggtg gcgatgtggt
     6181 cgatgttgac acccagcagg atgcggttgg cttcagtcac ggcgggactc cttgcgattg
     6241 atgaacagct cgcggctgac cagcggccgg ccgcccagat gcggcgccag cgcctggcgc
     6301 atcagccgct tggccgcgct cagggcgccc ggctgcgccc agttggcctc ggccagcgcc
     6361 agcagctcgt cgccgtggaa cagccccggc tgcagttggg cgaccggctg gaaacccagc
     6421 tcgggcaacc actggtacag cgtggcggga tcgaccggct gcccctggca gtcgcggtcg
     6481 agagcgaaac catagcccag atcatccagc agccgccatt cgaaggcgcg cagcagcggt
     6541 tccagcggcc gcccacccgc cagcgcggcg agggtcgcgg cgtagtggtc gaacagggcg
     6601 ggatgcgggt cttgcgccgg cagcaggcgc accagcagct cgttgagata caggccgctg
     6661 aacagcgcat ggcccgagag cagatgggga atgccggcac tttccaggcg ggcgacgttc
     6721 ggcaactcgc cgcggccacg caactccagt tccagcggca cgaacggccg cgccagcgtg
     6781 ccggccttgc cgcgcgcacc gcgcaacacc gcgcgcagac gcccttccgg agtcaggaaa
     6841 tccaccaagg cgctggtttc cttgtacggg cgggagtgca ggacgaaggc gggatgggcg
     6901 gcagcgatca tgtcaaccgt agggtgcgcc atgcgcaccg gcgaaagcct ggacagcggc
     6961 acaggtgcgc atggcgcacc ctaccacagc ggttgcaaac gccggtcaga tgtcgccgta
     7021 gcccagcgag cgcagggcgc gttcgtcgtc ggaccagccg cccttcacct tgacccacag
     7081 gttgagcatg accttgctgc cgaacagctc ttccatgtcg cggcgggctt cctggccgat
     7141 gcgcttgatg cgctcgccct tgtcgccaat gatgatcttc ttctgtccat cgcgctcgac
     7201 caggatcagc gcgtggatgt gcagcacgcg gccctgttgc tggaactgtt cgatttccac
     7261 ggcaacctgg tagggcacct cggcacccag ctggcgcatg accttctcgc gcaccagctc
     7321 ggcggcgagg aaccgcgagc tgcggtcggt gagctggtct tcctcgaaga agtgctcgcc
     7381 ttccggcagg tgcttggcca cctgggcctc gagggtctcg aggttctgcc cgtgttgcgc
     7441 ggagatcggc acgatctcgg cattgggcag ctgctcggcc agccacttca ggtgcggcag
     7501 cagctcaccc ttgtcctcga ggcggtcgat cttgttgacc accaccagca gcgggccttc
     7561 gacgtacttg acccggtcga ggaccatctg gtcctcctcg gtccagcgcg tgcggtcgac
     7621 gacgaagatc accacgtcga cgtccttcag cgccgcgctg gcgctgcggt tcatgtagcg
     7681 gttgagcgcc ttgtcgttat ccttgtgcat gccgggagtg tcgacgtaga tcgcctgcac
     7741 cgggccttcg gtcttgatgc cgagcagggt gtgccgggtg gtctgcggct tgcgcgaggt
     7801 gatcgccagc ttctgtccga ggatatggtt gagcagggtc gacttgccga cattcggccg
     7861 gccgacgatg gccacgtagc cgcaacggct cggcagggat tcagtcattg ccattctcca
     7921 cgcccaaggc caccagtgct gcagcggccg ccacctgttc ggcgatacga cggctggcac
     7981 cctggccacg ggtcttttga ttgagcaggg caatttcgca ttcgacgaag aaggtccggc
     8041 aatgcggctc accctggata tccaccacat cgtagcgcgg cagttcgcag ccacgcgatt
     8101 gcaggaactc ctgcaggcgg gttttcggat ccttgttggt atcgaccagg gtcagctcgt
     8161 cgaaataggg tgccagccag gccagcacgc gatcgcgcgc cgcctccatg ccggtgtcca
     8221 ggtagatggc gccgatcagc gcttcggtgg cgtcggcgag gatcgactcg cggcggaagc
     8281 cgccgctctt cagctcgccg gagccaaggc gcaggtattc gccgaggtcg aagaccagcg
     8341 ccaggcgcgc cagggtctcg cccttcacca gccgcgcacg caggcgtgac agctggcctt
     8401 cgcgggcctg gggaaagcgc ttgaacagcg cctcgccgac cacgaagttg aggatggcat
     8461 cgccgaggaa ctccagccgc tcgttgttgc ggccggcaaa gcttcggtgg gtcagggcca
     8521 ggaccatcag ctcagggtcc tggaatttat agccgagccg acgctcgagt cgttgcaggg
     8581 gattactcac ggggcacgga cacggtattc gcggtcaaag tggacaacca ggtcgaggtt
     8641 ttcgatcagc ggctcgcgct tctcgtagtt gaggtgaacg tagaactcgt cgttttccat
     8701 gctcacctcc atcatttcct tcaggtcaaa atcaagattg ttgacctcca ttccacgccc
     8761 catatgagcg taaaagtcgt tcggcgagcg aatctggtgg gcactttccg tagcggcgga
     8821 ggtaatgatt ttctgcagag agtagaagtc catgtagtgg ggaaaaacct ttagaaaaac
     8881 gctggcgata aatcccacga tgcaaaggac caccagccag ctcaacatcg acaagccttt
     8941 ctgcgaacgt gcaaacatca tgatcgacct cgtatccggg agtattcaac actcaatgga
     9001 tcagcccgac ccgcgagaac gtcggcaggt ggctgagctt gggctccggc cagctcatcc
     9061 acacggcgaa cgccttgccg acgatgttgc gatccggaac catgccatgc agcgcaagcg
     9121 gaatcgccgg at
//
LOCUS       DATCUN010000043         5213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_264552, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000043 DATCUN010000000
VERSION     DATCUN010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264552"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VIO83_01475"
     CDS             complement(<1..1259)
                     /locus_tag="VIO83_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8329989.1"
                     /translation="MINVDLQQLVQALDAATRRDLEGAAERCVARGGSKILVEDLLLG
                     LLERPDGLLARALQDAGVDAGELARVLQPRGEASASRNPVFATELVQWLQDALLAASL
                     ELGQSQIDQAALLLALLCSPLRHAGTRYQQLLSRLSTERLRDFARSQQPHPQPAAGQP
                     VADGESALARFTHNFTQQARDGKLDPVLCRDGAIRQMIDILARRRKNNPIVVGEAGVG
                     KTALVEGLALRIAGGEVPEALKGVELLGLDLGLLQAGASVKGEFERRLQGVIDGVKAA
                     PRPMILFIDEAHTLIGAGGQAGGSDAANLLKPALARGELRTIAATTWSEYKKYFEKDP
                     ALARRFQPVQLLEPTVAEAVTILRGLAPVYAQSHGIYLRDDAVVAAAELSARYLAGRQ
                     LPDKAVDVLDTACARVRISLASAPAAL"
     gene            complement(1256..2278)
                     /gene="tssG"
                     /locus_tag="VIO83_01480"
     CDS             complement(1256..2278)
                     /gene="tssG"
                     /locus_tag="VIO83_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEY8329990.1"
                     /translation="MDAAHGAAAPALNRLGRSIREYSLFQGVQRVLNRLQAAHPQLDE
                     QALYERLEFQANPSLGFPGCDIERVQFFEEHGELRARLCINLVGLCGAGSPLPAFYGE
                     QALGDSATDNPTRHFLDLFNSRLQRLLLPIWQKYRYHARFASGARDPLSAQLFALIGL
                     GGEQIRNASELNWKRLLPYLGLLSLRAHSAALIESVLRYYFKHAELHIEQCLERRVTI
                     LDEQRNRLGRANSQLGESLVLGERVRDRGGKFRIHIRQLGWQGFHDFLPLGDGYQPLC
                     ALVRFTLRDPLDYDIRLELRRDEIRELRIGAANPCRLGWTSWLGRDRADGLVTLGSRI
                     NQGAMK"
     gene            complement(2242..4032)
                     /gene="tssF"
                     /locus_tag="VIO83_01485"
     CDS             complement(2242..4032)
                     /gene="tssF"
                     /locus_tag="VIO83_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEY8329991.1"
                     /translation="MSFNHYYQSELNALRQLGKRFAERSPALAPFLGQSGRDPDVERL
                     LEGFAFLTGRLRQKLDDELPELTHSLMHLLWPNYMRPLPAFSMLQFEPLKRPGPALTV
                     PRHTPVESKPIQGATCRFRTAYATEVLPLALSALDYAVQGEGALLSLRLTMSADGHLG
                     ELDLRRLRLHLSGERYISQLLYLGLLRHLDGIQLVALDADGRPLRDAASEPLPPLRLD
                     PTQVQPVGFAEDEALIPYPLNTFRGYRYLQEYFAFQDKFLFVDLHGLEVLRRLPDEQL
                     KQTRGLELRFDIRQAGVQRIRPTLDNLRLYCTPVVNLFAHDATPIRLDGRQDQYLLRP
                     AEYDPQHCAVFSVDRVTGWRPGGMGYEEYVPFESFEHDASFDVPHARPHYSVRQQPSL
                     LGDGLETWLSFGARQADQQETLSIELTCTNQNLPRQLGIGDICRPCEGTPEFLAFRNI
                     GAVTPSYAPPLHRDYLWKLISNMSLNYLSLANVEELKAILETYDLPRHYDQHAEKVSR
                     RLLGGLKQIDHRHVDRLHRGLPVRGVRTELVMAADCYLGEGDLFLFASVLNEFFALYA
                     SLNSYHELRVQSTQGEVYQWTPRMGQQPLL"
     gene            complement(4061..4294)
                     /locus_tag="VIO83_01490"
     CDS             complement(4061..4294)
                     /locus_tag="VIO83_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329992.1"
                     /translation="MERHEVQMLMSIQGYPSVLADSDLFGCLAILSFLAGCSGLFARI
                     RNTRTHRALTFACLMAVPALHAVGTFFLNQAPA"
     gene            complement(4391..5023)
                     /locus_tag="VIO83_01495"
     CDS             complement(4391..5023)
                     /locus_tag="VIO83_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329993.1"
                     /translation="MNPFAFLHRKPSAKQLLHQGRRAELQELISVYLPRSLALPKQGL
                     GYPCVLSLKVDGLGAYLLDVHLDTEHLTNIEQLIERLGFNRQQPTAKFHTFSRQCPAP
                     EAVSIQRQKLGQGTALLLISNSIELLQNLPELAPPKPWEVFPEVDADGLGSLQGSLEH
                     WWNHYWWPYWLNLTPKQRASWLDDARHPEGWREYIRLQDELNGTNREPQA"
     gene            complement(5026..>5213)
                     /locus_tag="VIO83_01500"
     CDS             complement(5026..>5213)
                     /locus_tag="VIO83_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HEY8329994.1"
                     /translation="IRQAGGWGAAWAGFKVGGAAGAALGIETGPGAVISGLIGGIVVG
                     AAGYFGADWVADHIYEN"
BASE COUNT          825 a         1643 c         1876 g          869 t
ORIGIN      
        1 tccagcgccg cgggcgcgct ggccaggctg atgcgcaccc gcgcgcaggc ggtgtccagc
       61 acgtcgactg ccttgtccgg cagctgacgg ccggcgaggt agcgggccga cagttcggcg
      121 gcggccacca cggcatcgtc gcgcaggtag atgccgtggc tctgcgcgta gaccggcgcc
      181 aggccgcgca ggatggtcac cgcctcggcc accgtcggct cgagcagctg caccggttgg
      241 aagcggcgag ccagcgccgg gtccttctcg aagtacttct tgtattcgct ccaggtggtg
      301 gcggcgatgg tgcgcagctc gccacgcgcc agcgccggct tgagcaggtt ggcggcgtcc
      361 gagccgccgg cctggccacc ggcgccgatc agggtgtgcg cctcgtcgat gaacaggatc
      421 atcggcctgg gcgcggcctt caccccgtcg atcaccccct gcaggcggcg ttcgaactcg
      481 cccttgacgc tggcgccggc ctgcagcaga ccgagatcga ggccgagcag ctcgacgccc
      541 ttgagcgcct ccggcacctc gccgccggcg atgcgcaggg ccaggccctc gaccagcgcg
      601 gtcttgccga cgccggcctc gccgaccacg atggggttgt tcttccgccg gcgggcgagg
      661 atgtcgatca tctggcggat cgcgccgtcg cggcacagca ccgggtcgag cttgccgtcc
      721 cgggcctgct gggtgaagtt gtgggtgaag cgtgccagcg ccgattcgcc gtccgccacg
      781 ggctggccgg cggccggctg cggatgcggc tgctggctgc gggcgaagtc gcgcaggcgc
      841 tcggtgctca gccgggacag cagctgctgg tagcgggtgc cggcatggcg cagcgggctg
      901 cacagcaggg cgaggagcag ggcggcctgg tcgatctggc tctggccgag ctccaggctg
      961 gcggccagca gggcatcctg cagccactgc accagttcgg tggcgaatac cgggttgcgc
     1021 gacgcgctgg cctcgccgcg cggctgcagc acgcgggcca gttcgccggc atccaccccg
     1081 gcatcctgca gggcgcgggc cagcaggccg tccgggcgct cgagcaggcc gagcagcaga
     1141 tcctcgacca ggatcttgct gccgccgcgg gccacgcagc gctcggcggc gccttccagg
     1201 tcgcggcggg tcgcggcatc gagggcctgg accagctgtt gcaaatcgac gttgatcatt
     1261 tcatcgctcc ttgattgatg cggctgccca gggtgaccag gccgtcggcg cggtcgcggc
     1321 cgagccagct ggtccagccc aggcggcagg ggttggcggc gccgatgcgc agctcgcgga
     1381 tttcgtcccg gcgcaattcc aggcggatgt cgtagtcgag cgggtcgcgc agggtgaagc
     1441 gcaccagcgc acacagcggc tggtagccgt cgcccagcgg caggaaatcg tggaagcctt
     1501 gccagccgag ctggcggatg tggatgcgga acttgccgcc gcggtcgcgc acccgctcgc
     1561 cgagcaccag gctttcgccc agctggctgt tggcgcggcc gaggcggttg cgctgttcgt
     1621 cgaggatggt cacccggcgc tccaggcact gttcgatgtg cagctcggcg tgcttgaagt
     1681 agtagcgcag caccgattcg atcagcgctg ccgaatgggc gcgcaggctg agcaggccga
     1741 ggtagggcag caggcgtttc cagttcagct ccgatgcgtt gcggatctgc tcgccgccga
     1801 ggccgatcag cgcgaacagc tgggcggaca gcggatcgcg ggcgccgctg gcgaagcgcg
     1861 cgtggtagcg gtacttctgc cagatcggca gcagcaggcg ctgcaggcgg ctgttgaaca
     1921 ggtcgaggaa gtggcgggtc gggttgtcgg tggcgctgtc gcccagcgcc tgttcgccgt
     1981 agaacgccgg cagcggcgag ccggcaccgc acaggccgac caggttgatg cacaggcgcg
     2041 cgcgcagctc gccgtgctcc tcgaagaact gcacgcgctc gatgtcgcag ccgggaaagc
     2101 ccaggctggg gttggcctgg aattccaggc gctcgtacag cgcctgctcg tccagctgcg
     2161 gatgggcagc ctgcaggcgg ttcagcaccc gctgcacgcc ctggaacagg ctgtactcgc
     2221 ggatgctccg gccgagccgg ttcagagcag gggctgctgc cccatgcgcg gcgtccattg
     2281 gtacacctct ccctgagtgc tctgcacgcg cagctcgtgg tacgagttga ggctggcgta
     2341 cagggcgaag aattcgttga gcaccgaggc gaagaggaac aggtcgccct cgcccaggta
     2401 gcagtcggcg gccatgacca gctctgtgcg cacgccgcgc accggcaagc cgcggtgtag
     2461 gcggtcgacg tgccggtggt cgatctgctt gagcccgccg agcaggcgcc ggctgacctt
     2521 ttccgcgtgc tggtcgtagt ggcgcggcag gtcgtaggtc tcgagaatgg ccttgagctc
     2581 ctcgacgttg gccagcgaca ggtagttgag cgacatgttg gaaatcagct tccacaggta
     2641 gtcgcggtgc agcggcggtg cgtagctggg ggtcaccgcg ccgatgttgc ggaacgccag
     2701 gaactccggg gtgccctcgc agggccggca gatgtcgccg atccccagtt ggcgcggcag
     2761 gttctggttg gtgcaggtca gctcgatgga cagcgtctcc tgctggtcgg cctggcgcgc
     2821 gccgaagctc agccaggtct ccaggccgtc gccgagcagc gagggctgct ggcgcacgct
     2881 gtagtgcggg cgggcgtggg gcacgtcgaa gctggcgtcg tgctcgaacg actcgaacgg
     2941 cacgtattcc tcgtagccca tgccgccggg ccgccagccg gtgacccggt ccaccgagaa
     3001 caccgcgcaa tgctgcgggt cgtattcggc cgggcgcagc aggtactggt cctggcgacc
     3061 gtcgaggcgg atcggcgtgg cgtcgtgggc gaacaggttg accaccgggg tgcagtacag
     3121 gcgcaggttg tccagggtcg ggcggatccg ctgcacgccg gcctggcgga tgtcgaagcg
     3181 cagctccagg ccgcgcgtct gcttgagttg ctcgtccggc aggcgtcgga ggacctctag
     3241 cccgtgcagg tcgacgaaca ggaacttgtc ctggaaggcg aagtattcct gcaggtagcg
     3301 atagccgcgg aaggtgttga gcgggtaggg aatcagcgcc tcgtcctcgg cgaagccgac
     3361 cggttgcacc tgcgtgggat ccaggcgcag gggcggcagc ggctcgctgg cggcatcgcg
     3421 cagcggccgg ccgtcggcgt cgagggcgac cagctggatg ccgtcgaggt ggcgcagcag
     3481 gccgaggtag agcagctggc tgatgtagcg ctcgccactc aggtgcaggc gcagccggcg
     3541 caggtccagc tcgccgagat ggccgtcggc gctcatggtc aggcgcaggc tgagcagcgc
     3601 gccctcgccc tgtaccgcgt agtcgagggc gctcagcgcc agcggcaaca cctcggtggc
     3661 gtaggcggtg cggaagcggc aggtcgcgcc ctggatcggc ttcgactcca ccggcgtgtg
     3721 gcggggtacc gtcagcgccg gaccgggacg cttgagcggc tcgaactgca gcatgctgaa
     3781 cgccggcagc gggcgcatgt agttcggcca cagcaggtgc atcagcgagt gggtcagctc
     3841 cggcaactcg tcgtcgagct tctggcgcag gcgcccggtg aggaaggcga agccctcgag
     3901 caggcgctcg acatccggat cgcgcccgct ctggccgagg aacggcgcca gcgccgggct
     3961 gcgctcggcg aagcgcttgc cgagctggcg cagggcgttg agttcgctct ggtagtagtg
     4021 gttgaaggac atcaggccta tccctggctt attgattgga tcaggctggt gcctgattca
     4081 ggaaaaatgt tccaactgca tgcaaggctg gaacggccat caagcaggca aaggtcagag
     4141 ctctgtgcgt tctggtgttg cgtatccggg cgaaaagacc agagcagcca gcaagaaagc
     4201 tcagaatggc cagacagccg aacagatcac tgtcggccag tacggagggg tagccttgaa
     4261 tgctcatcag catttgcacc tcatggcgtt ccatcgaggg ttcgcgccag cgccgggcaa
     4321 cgtccggcga aacgcttgcc gagcgggcgc tgggcggaga gttcgcgctg gtagtagtgg
     4381 ttgaagggca tcaggcctgt ggctccctgt tggtgccgtt cagctcatcc tgtagacgga
     4441 tatattcgcg ccagccttcg ggatgccggg cgtcatcgag ccagcttgca cgctgcttcg
     4501 gagtgaggtt cagccagtac ggccaccagt agtggttcca ccagtgttcg aggctaccct
     4561 gcaggctgcc aagcccatcg gcatctacct cggggaatac ttcccagggc tttggcggcg
     4621 ctagttctgg caggttttgc aggagttcga tgctgttgct gataagcagc agggccgttc
     4681 cctggcctaa tttttgcctt tgaatcgaaa cggcctcggg ggccggacat tggcggctga
     4741 acgtgtggaa cttcgcggta ggctgttgcc gattgaatcc gagcctctca atcaattgtt
     4801 caatattggt gaggtgctcc gtgtccagat gcacatccag cagataagcc ccaaggccgt
     4861 cgaccttgag cgacagcaca caggggtagc ccagaccttg cttaggcagt gccaaactcc
     4921 gaggcagata caccgaaatc agttcctgca gttctgctcg tcgtccctga tgaagcaatt
     4981 gcttagccga tggcttcctg tgcaggaagg caaatgggtt catgttcaat tctcgtagat
     5041 gtggtcggcg acccagtcag caccgaaata gccagctgcg ccgacaacga tgcccccgat
     5101 caggccgcta ataactgcac ctggaccggt ttctataccc agagctgcac cggcggcacc
     5161 accgaccttg aaaccagccc aagctgcacc ccatccaccg gcctggcgga tag
//
LOCUS       DATCUN010000044         1778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_151911, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000044 DATCUN010000000
VERSION     DATCUN010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151911"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /gene="ssrA"
                     /locus_tag="VIO83_01505"
     tmRNA           <1..140
                     /gene="ssrA"
                     /locus_tag="VIO83_01505"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            469..966
                     /locus_tag="VIO83_01510"
     CDS             469..966
                     /locus_tag="VIO83_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEY8329995.1"
                     /translation="MPGLSLSAPAAARIARLLLAGLLALALSACAALGSREPINMHVV
                     GFDPLPGEGLEMRFAVKLRLQNPNTTPIEYDGIALELEVGGRALATGVSDQRGSVPRF
                     GEKLLVVPVSISAFSAVRQALGLAETDRLDKVPYVLHGKLSGGAFGSRRFSEKGTLNL
                     SRVGR"
     gene            complement(973..1164)
                     /locus_tag="VIO83_01515"
     CDS             complement(973..1164)
                     /locus_tag="VIO83_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8329996.1"
                     /translation="MSTLTELAQQIALLYPLRDKTAGKRYRIVNELAGLTELEEIGGK
                     PRYIPTRALQDRKIWDLAG"
     gene            complement(1272..>1778)
                     /locus_tag="VIO83_01520"
     CDS             complement(1272..>1778)
                     /locus_tag="VIO83_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY8329997.1"
                     /translation="GVTRGALYWHFENKAHLFQAMLEQVRLPPEQLAERLSTCGDRDP
                     LQVLREMCSEAITSLARNPQKRRIFTILLHRCEFTDELRHVEERHEAFVNQFIDLCEE
                     LFERADRQGRLNPGQTPRLAARSLHALIIGLFSDWLRDPELFDAERDAPQMIDALLRG
                     LARDWPTG"
BASE COUNT          263 a          534 c          634 g          347 t
ORIGIN      
        1 aacggctaaa actcatgcag ctcgctccaa gcaccctgcc agtcgggtcg cgaggagtta
       61 actcaataga catggctaag catgtagaac cgatagtgga gagctggcgg acgggggttc
      121 aaatcccccc ggctccacca aaacctgaca caaagccccg aactccgggg ctttgtgctg
      181 tctgggcctg ccattttcgg caggtgtgcc actgacaggt cacaccgacg tggggcatcg
      241 gcaggtgccc agcatgaagc ctgccccttc ctacctggcc cggaaccgcc acggaatctt
      301 ccatttccgc atggtgattc cgtcgaagct cttcttctcc cgctgctgtt tctgctccgg
      361 cgcctggctt ggccgggtcg gtggtggaat attgctggca ggtgccgcgc aggttgcgtt
      421 cggcaggccg atacttggcc ttatccgccc gcatcaggac gtcatgccat gcccggtctt
      481 tctctttctg ctccggccgc tgcccgtatc gcccgcctcc tgctggccgg gctgctggcg
      541 ttggcgttga gtgcctgcgc cgcgctcggc agtcgcgagc cgatcaacat gcatgtggtg
      601 ggcttcgatc ccttgccggg ggagggcctg gagatgcgct ttgcggtgaa gctgcgcctg
      661 caaaacccga acaccacgcc gatcgagtac gacggcattg cgctggagct ggaggtcggc
      721 ggccgcgcgc tggccacggg ggtgagcgat cagcgtggca gtgtgccgcg cttcggtgag
      781 aagctgctgg tggtgccggt cagcatctcg gcgttttccg ccgtgcgtca ggcgctgggg
      841 ctggccgaga ctgatcgctt ggacaaggtg ccctacgtgc tgcatggcaa gctgtccggt
      901 ggggcgttcg gcagtcggcg cttctctgaa aagggcacgc tgaatctgtc gagggtgggg
      961 cgctaggggc gatcagccgg ccagatccca gatcttgcga tcctggaggg cgcgggtcgg
     1021 gatgtagcgc ggtttgccgc cgatctcctc cagctcggtc aggccggcga gttcgttgac
     1081 gatgcgatag cgcttgccgg ccgtcttgtc cctgagtggg tacagcaggg cgatctgttg
     1141 ggccagttcg gtgagggtgg acatgtcgct gctccgtgaa tcgatggcag ctgtttaccg
     1201 gatgcgggtt gcaggcgtgt gaacgtcggc gaaagccgtg ggctgcaggg catccggccg
     1261 aggcgggtgg ctcagccggt cggccagtcg cgcgccagtc cgcgcagcag ggcatcgatc
     1321 atctgcggtg cgtcgcgttc ggcgtcgaac agttcggggt cgcgcagcca gtcgctgaac
     1381 aggccgataa tcagcgcgtg caggctgcgg gcggccaggc gcggagtctg gccggggttc
     1441 aggcggccct ggcggtcggc gcgctcgaaa agctcctcgc agagatcgat gaactgattg
     1501 acgaaggctt cgtggcgctc ctcgacgtgg cgcagttcgt cggtgaactc gcagcggtgc
     1561 agcaggatgg tgaagatccg ccgcttctgc gggttgcgcg ccaggctggt gatggcttcg
     1621 ctgcacattt cgcgcaggac ctgcagcgga tcgcggtcgc cgcaggtgga caggcgctcg
     1681 gccagctgct ccggcggcag gcgcacttgc tcgagcatgg cctggaacag gtgggccttg
     1741 ttctcgaagt gccagtagag cgcgccgcgg gtcacgcc
//
LOCUS       DATCUN010000045         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_76949, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000045 DATCUN010000000
VERSION     DATCUN010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76949"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1016)
                     /locus_tag="VIO83_01525"
     CDS             complement(51..1016)
                     /locus_tag="VIO83_01525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020289880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8329998.1"
                     /translation="MNADAIATLQQQVIAALGAVPDETRRLFHGRGRRWPGLEHVTVD
                     WLQGVLLVALFREPEAAELAMLKQMLMALSQTAEWRHSQAHSLLLQHRYRLDSSTEWL
                     LGDEVEEWLISEDGLRFKLDLGKKQNTGLFLDMRYGRRWVREQAAGKRVLNLFAYTCG
                     FSVAALAGGAEQVVNLDMAKAALNRGRDNHRLNQQDLSQVSFLGHELFKSWSKVKKYG
                     PYDLIIIDPPSFQKGSFALTKDYQKILRRLPDLLTAQGVVLACINDPAIGPDFLIEGM
                     ASEAPSLTFEMRLENQPEFADIDPDAGLKALVFKHNPLVAATPVA"
     gene            1129..1554
                     /locus_tag="VIO83_01530"
     CDS             1129..1554
                     /locus_tag="VIO83_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEY8329999.1"
                     /translation="MSNNPSYGVGDASYQAAGGIDGLRRLVDDFYRIMDESPAAADLR
                     RMHPESLDASRDKLACFLSGWLGGPKLFAAKYGSIVIPAFHAQWPIDEAGSAAWLDCM
                     AQAIARQGYAPEFAEYLLGQLRVPAQRIVQASRARHGQS"
     gene            complement(1562..3571)
                     /gene="rep"
                     /locus_tag="VIO83_01535"
     CDS             complement(1562..3571)
                     /gene="rep"
                     /locus_tag="VIO83_01535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEY8330000.1"
                     /translation="MSRLNPRQQEAVHYISGPLLVLAGAGSGKTSVITRKIAYLIQQC
                     GIRAQNIVAVTFTNKAAREMKERVSSLLRGSEGKGLTVSTFHNLGLNIIRKEHAHLGY
                     KPGFSIFDEQDVKALLADIMQKEYQGDDGVDEIKNLIGAWKNDLILPEQALEQARNPR
                     EQTAAIVYLHYQRTLKAYNAVDFDDLILQPVKLFQDHPEVLQKWRNKVRYMLVDEYQD
                     TNASQYLLVKLLVQERAQFTVVGDDDQSIYAWRGARPENLMLLKDDFPSLKVVMLEQN
                     YRSTSRILKCANVLIANNPHVFEKQLWSEMGHGDEIRVIRCRNEDAECERIAMEILTQ
                     HLRTDRPYSEFAILYRGNYQAKLMELKLQHHQIPYRLSGGTSFFARQEVKDLMSYFRL
                     LVNPDDDNAFLRVINVPRREIGSATLEKLGNYATARKISMYDASDEIGLAEHLDSRYC
                     ERLARFKHFIDRVRRECAQDDPIAALRGMIADIDYENWLRENSSTDKAADYRMSNVWF
                     LLEALKNTLEKDEDGDMTIEQAIAKLVLRDMLERQQEEEDGAEGVQMMTLHASKGLEF
                     PCVFIMGMEEEILPHRSSIEADTVEEERRLAYVGITRAKQNLAITFAAKRKQYGEVID
                     CAPSRFLDELPAEDLQWEGLEDTPVEVKAVRGNSALADIRALLKR"
BASE COUNT          518 a         1146 c         1239 g          698 t
ORIGIN      
        1 gcgagtcgcc agccacggtt gcccgagcgc tgcccggccg cgcggcgcgg ctacgcgacc
       61 ggagtggcgg cgacgagggg gttgtgtttg aacaccaggg ccttcagccc ggcgtcgggg
      121 tcgatgtcgg caaactcggg ctggttttcc aggcgcatct cgaaggtcag gctcggcgcc
      181 tcgctggcca tgccttcgat caggaagtcg ggaccgatgg caggatcgtt gatgcaggcc
      241 agtaccaccc cctgcgcggt gagcaggtcg ggcaggcggc gcaggatctt ctgatagtcc
      301 ttggtcaggg caaagctgcc cttctggaac gacggcgggt cgatgatgat caggtcgtag
      361 gggccgtatt tcttcacctt gctccatgac ttgaacagct catggccgag gaagctgacc
      421 tgactgaggt cctgctggtt cagccggtga ttgtcgcgcc cgcggttgag ggcggccttg
      481 gccatgtcca gattgaccac ctgctcggcg ccaccggcaa gcgccgccac cgagaaaccg
      541 caggtatagg cgaacaggtt gagcacgcgc ttgcccgcgg cctgttcccg tacccagcgc
      601 cggccatagc gcatgtcgag aaacaggccg gtattctgct tcttgcccag atccagcttg
      661 aagcgcaggc cgtcctcgct gatcagccac tcttccacct cgtcgcccag cagccactcc
      721 gtgctgctgt ccagccggta gcggtgctgc agcaacaggc tgtgcgcctg gctgtgccgc
      781 cactcggcgg tttggctcag ggccatgagc atctgcttga gcatggccag ttccgccgcc
      841 tccggctcgc ggaacagcgc caccagcagc acgccctgca gccagtccac ggtgacatgc
      901 tcgagccccg gccagcgccg cccgcggccg tggaacaggc gccgggtctc gtcgggaacg
      961 gcgcccaggg cggcaatgac ctgctgctgc agggtggcta tggcatcggc attcatggct
     1021 agtcgtcagt gatcggtgat ggcgcatttt aagcgctgcg tcgggcggct gaggacaaaa
     1081 tcctttccgg ctaagatggg cctttccgta cccagtgaga gcgcccccgt gagcaacaat
     1141 ccctcctatg gtgtcggcga cgcctcgtac caggccgccg gcggtatcga cggcctgcgc
     1201 cgactggtcg acgacttcta ccggatcatg gacgagtcgc ccgccgccgc cgacctgcgc
     1261 cgcatgcacc ccgagagtct cgatgcctcg cgcgacaagc tggcctgctt cctcagcggc
     1321 tggctgggcg ggccgaagct gtttgccgcg aagtacggct cgatcgtgat tcccgccttc
     1381 catgcgcagt ggccgatcga cgaggccggc agcgcggcct ggctggactg catggcgcag
     1441 gccatcgccc gccagggcta tgcgccggag ttcgccgagt acctgctggg gcagctgcgc
     1501 gtgccggcgc agcgcatcgt ccaggccagc cgcgcgcgcc acggccagtc ctgagcgcgc
     1561 ctcagcgctt gagcagcgcg cggatgtccg ccagcgcgct gttgccgcgc accgccttga
     1621 cctccaccgg ggtgtcctcc aggccttccc actgcaggtc ctcggccggc agttcgtcga
     1681 ggaagcggct gggcgcgcag tcgatcacct cgccgtactg cttgcgcttg gcggcgaagg
     1741 tgatggcgag gttctgcttg gcgcgggtga tgccgacgta ggccaggcgg cgctcttcct
     1801 cgacggtgtc ggcctcgatg ctggagcggt gcgggaggat ttcctcctcc atgcccatga
     1861 tgaacacgca ggggaactcc aggcccttgg aggcgtgcag ggtcatcatc tgcacgccct
     1921 cggcgccgtc ctcctcctcc tgctggcgtt cgagcatgtc gcgcagcacc agcttggcga
     1981 tggcctgctc gatggtcatg tcgccgtcct cgtccttctc cagggtgttc ttcagcgcct
     2041 cgaggaggaa ccagacgttg ctcatccggt agtcggcggc cttgtcggtg gagctgttct
     2101 cgcgcagcca gttctcgtag tcgatatcgg cgatcatccc gcgcagcgcg gcgatcgggt
     2161 cgtcctgggc gcactcgcgg cgcacccggt cgatgaagtg cttgaagcgc gccaggcgct
     2221 cgcagtagcg gctgtcgagg tgctcggcca ggccgatctc gtcgctggcg tcgtacatgc
     2281 tgatcttgcg cgcggtggcg tagttgccga gcttctccag ggtcgccgag ccgatctcgc
     2341 ggcgtggcac gttgatcacc cgcaggaagg cgttgtcgtc gtccgggttg accagcagac
     2401 ggaagtagct catcaggtcc ttcacctcct gacgggcgaa gaagctggtg ccgccggaca
     2461 gtcgataggg aatctggtgg tgctgcagct tcagctccat cagcttggcc tggtagttgc
     2521 cgcggtagag gatggcgaac tcgctgtacg ggcggtcggt gcgcaggtgc tgggtgagga
     2581 tctccatggc gatgcgctcg cactcggcgt cctcgttccg gcagcggatc acgcggatct
     2641 cgtcgccgtg acccatctcg ctccacagct gcttctcgaa cacgtgcggg ttgttggcga
     2701 tcagcacgtt ggcgcacttg aggatgcggc tggtcgagcg gtagttctgc tcgagcatca
     2761 ccaccttgag gctggggaaa tcgtccttga gcagcatgag gttttccggc cgcgcgccgc
     2821 gccaggcgta gatcgactgg tcgtcgtcgc cgaccacggt gaactgcgca cgctcctgga
     2881 ccagcagctt gaccagcagg tactggctgg cgttggtgtc ctggtattcg tcgaccagca
     2941 tgtagcgcac cttgttgcgc cacttctgca gcacctcggg gtggtcttgg aacagcttga
     3001 ccggctgcag gatgaggtcg tcgaagtcca ccgcgttgta ggccttgagg gtgcgctggt
     3061 agtgcaggta gacgatggcg gcggtctgct cgcgcgggtt gcgcgcctgc tccagggcct
     3121 gctcggggag gatcaggtcg ttcttccagg cgccgatcag gttcttgatc tcgtcgacgc
     3181 cgtcgtcgcc ctggtactcc ttctgcatga tgtcggcgag cagcgccttg acgtcctgct
     3241 cgtcgaagat cgagaagccc ggcttgtagc ccaggtgggc gtgttccttg cggatgatgt
     3301 tgaggccgag gttgtggaag gtcgacaccg tcaggccctt gccctcgctg ccgcgcagca
     3361 ggctggagac gcgctccttc atctcgcggg cggccttgtt ggtgaaggtc accgcgacga
     3421 tgttctgcgc gcggatgccg cactgctgga tcaggtaggc gatcttgcgg gtgatcacgc
     3481 tggtcttgcc ggatccggcg ccggccagca ccagcagcgg gccgctgatg tagtggacgg
     3541 cttcctgctg gcggggattg agtcgggaca tgcgggcagg ggtgtctggc tgcgaaggag
     3601 g
//
LOCUS       DATCUN010000046         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_152221, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000046 DATCUN010000000
VERSION     DATCUN010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152221"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1263
                     /locus_tag="VIO83_01540"
     CDS             19..1263
                     /locus_tag="VIO83_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY8330001.1"
                     /translation="MAQVINTNTMSLNAQRNLSTSGSSLATTIQRLSSGSRINSAKDD
                     AAGLAISERFGTQIRGTDVAIRNANDGISLAQVAEGSLTEIGNNLQRVRELSVQASNA
                     TNSASDRKALQAEVTQLVSEIDRVAKQSDFNGTKLLDGSFSSQLFQVGANAGQAIAID
                     KVVDAKASSLGNTTFANAYKGTALDAAGTAGVNEDLATADTVFTGLQVSITPKDGTAA
                     AINIGDISVKAGESISKATAAAVNAKLGETGVMASVDSSGVISLSSVKNGQTFELAAG
                     GPATAGATAVAADFGNIGLEESSTDGGTMTGATASVLSSVDISTVEGAQKALEIVDSS
                     LSAINNTRADLGAIQNRFTSVVANLQTSTENLSASRSRIRDTDFAKETAELTRTQILQ
                     QAGTAMLAQANQVPQNVLSLLR"
     gene            1345..>2427
                     /locus_tag="VIO83_01545"
     CDS             1345..>2427
                     /locus_tag="VIO83_01545"
                     /inference="COORDINATES: protein motif:HMM:NF006467.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY8330002.1"
                     /translation="MAQVINTNTMSLNAQRNLSTSGSSLATTIQRLSSGSRINSAKDD
                     AAGLAISERFGTQIRGTDVAIRNANDGISLAQVAEGSLTEIGNNLQRVRELAVQSSNA
                     TNSASDRKALQAEVTQLVSEIDRVAKQSDFNGTKLLDGSFSSQLFQVGANAGQAIAID
                     KIVDATTAKLGTSTFATGKVADATAALTADATLTGTIKVGSATVTLDKIELKSGATVS
                     DQNKAAVTAINSKLGETGVLAELDDTGKIVVTQVKDGVAMTAADITLTGAAGFAHTAD
                     PAVTTANVANVDVSTVTGAQKALSLVDNALSSINNTRADLGAVQNRFTSVVANLQTST
                     ENLSASRSRIRDTDFAKETAELTRTQI"
BASE COUNT          503 a          871 c          690 g          363 t
ORIGIN      
        1 gctcaaggac ctcttcccat ggcacaagtc atcaatacca acacgatgtc gttgaatgct
       61 cagcgtaacc tgagcaccag cggcagctcc ctggccacca ccatccagcg cctgtcgtcg
      121 ggttcgcgca tcaacagcgc caaggacgat gcggctggcc tggccatctc cgagcgtttc
      181 ggcacccaga tccgcggtac cgacgtggcc atccgcaatg ccaacgacgg catctcgctg
      241 gcccaggtcg ccgaaggctc gctgaccgaa atcggcaaca acctgcagcg cgtccgcgaa
      301 ctgtcggtgc aggcgtccaa cgccaccaac tcagctagcg accgcaaggc cctgcaggcc
      361 gaagtcaccc agctggtcag cgaaatcgac cgcgtggcca agcagtccga cttcaacggc
      421 accaagctgc tggacggctc gttctccagc cagctgttcc aggtcggcgc caacgccggc
      481 caggccattg cgatcgacaa ggtcgtagac gccaaggcca gctcgctggg caataccacg
      541 ttcgccaatg cctacaaggg taccgccctc gatgccgccg gcaccgcggg tgtcaacgaa
      601 gacctggcca cggccgatac ggtgttcacc ggccttcagg tatcgatcac cccgaaggac
      661 ggcactgccg ccgccatcaa tatcggcgat atctcggtaa aggccggtga gtcgatctcc
      721 aaggctaccg ccgccgcggt caacgccaag ctgggcgaaa ccggcgtgat ggccagcgtt
      781 gacagctcgg gtgtgatcag cctcagctcg gtcaagaatg gccagacctt cgaactggct
      841 gctggtggtc cggctacggc aggcgcaacc gccgtggctg ctgactttgg caacatcggc
      901 ctggaagaga gcagcaccga tggcggcacc atgaccggtg caaccgccag cgtgctgtcc
      961 tcggtagaca tctccaccgt cgaaggtgca cagaaggcgc tggagatcgt ggacagctcg
     1021 ctgagcgcga tcaacaacac ccgtgctgac ctgggcgcca tccagaaccg cttcacctcg
     1081 gttgtggcca acctgcagac ctccacggag aacctgtcgg cctcgcgtag ccgcatccgc
     1141 gataccgact tcgccaagga aaccgcagag ctgacccgca cccagatcct gcagcaggcc
     1201 ggtacggcca tgctggcaca ggccaaccag gtgccgcaga acgtgctcag cctgctgcgc
     1261 tgatccagcc gctccacaac ggcgccgggc tgaccggcgc cgtgtccacc gcctcacgtt
     1321 tcacccaagc ctgaaggaat ccccatggca caagtcatca acaccaacac gatgtcgttg
     1381 aatgctcagc gtaacctgag caccagcggc agctcgctgg ccaccaccat ccagcgcctg
     1441 tcgtccggtt cgcgcatcaa cagcgccaag gacgacgcgg ctggtctggc catctccgag
     1501 cgtttcggca cccagatccg cggcaccgac gtcgccatcc gcaatgccaa cgacggcatc
     1561 tcgctggccc aggtcgccga aggttcgctg accgaaatcg gcaacaacct gcagcgcgtc
     1621 cgcgaactgg ccgtgcagtc atccaacgcc accaactcgg ccagtgaccg caaggccctg
     1681 caggccgaag tgacccagct ggtcagcgaa atcgaccgcg tcgccaagca gtccgacttc
     1741 aacggcacca agctgctgga cggttcgttc tccagccagc tgttccaggt cggcgccaac
     1801 gccggccagg cgatcgccat cgacaagatc gtcgatgcca cgaccgccaa gctgggtacg
     1861 tcgaccttcg ctaccggcaa ggtggctgat gcgacggccg ccctgaccgc cgatgccacc
     1921 ctgaccggca ccatcaaggt cggttcggcc accgtcacgc tggacaagat cgaactgaag
     1981 agcggcgcca ccgtttccga tcagaacaag gctgccgtca ccgccatcaa ctccaagctc
     2041 ggcgaaaccg gcgtgctggc agagctggat gacaccggca agatcgtggt cacccaggtc
     2101 aaggacggcg tcgcaatgac cgccgccgac atcaccctga ccggtgctgc tggttttgcg
     2161 cacactgccg accctgcggt taccaccgcc aacgtcgcca atgtggacgt ctccactgtc
     2221 accggtgcgc agaaggcact gtcgctggtc gacaacgcgc tgagctcgat caacaacacc
     2281 cgcgcagacc tgggcgccgt gcagaaccgc ttcacctcgg tcgttgccaa cctgcagacc
     2341 tccacggaga acctgtcggc ctcgcgtagc cgcatccgcg ataccgactt cgccaaggaa
     2401 accgcagagc tgacccgcac ccagatc
//
LOCUS       DATCUN010000047        12159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_39717, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000047 DATCUN010000000
VERSION     DATCUN010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 12159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12159
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39717"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="VIO83_01550"
     CDS             complement(<1..149)
                     /locus_tag="VIO83_01550"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019124.1"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7 phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate, tyrosine
                     sensitive; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY8330003.1"
                     /translation="MNVSASARLATPQSPARRSAQPLPSPALLRQRLPLSDTLAARIQ
                     TDRNA"
     gene            complement(613..1437)
                     /gene="ttcA"
                     /locus_tag="VIO83_01555"
     CDS             complement(613..1437)
                     /gene="ttcA"
                     /locus_tag="VIO83_01555"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEY8330004.1"
                     /translation="MGTLSVNQNKLQKRLRRLAGEAVTDFNMIEDGDKVMVCLSGGKD
                     SYTMLDVLLYLQKVAPIKFEIVAVNMDQKQPGFPEHVLPEYLKSIGVDYHIIEKDTYS
                     VVKEKIPEGKTTCSLCSRLRRGTLYTYADEIGATKMALGHHRDDILETFFLNMFYGGT
                     LKAMPPKLLSDDGRNVVIRPLAYCSEKDIEAYAQLKEFPIIPCNLCGSQENLQRQVVK
                     EMLHEWERKSPGRTEIMFRALQNVVPSQLADRNLFDFQSLKIDDDATPRFIDVMSL"
     gene            1614..2207
                     /locus_tag="VIO83_01560"
     CDS             1614..2207
                     /locus_tag="VIO83_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-J related domain-containing protein"
                     /protein_id="HEY8330005.1"
                     /translation="MNSLFDPDLELVEQLLTLLRQSPDGLSEHQLIHLLKSGHCTHIP
                     NLPLRDRLVLFRTHFLVFNALYRLRDRLWAEQSAHLEINPLLIRLLPYRDGAQGVVEH
                     DALRAYYLDETHLRDTSEADVEHLLQSFWTRMHHGEEQQAALELFGLDARTDYPAIRL
                     RYRQLVSQHHPDRGGSTTRLQSINKAMEILDRYYNPR"
     gene            2365..2964
                     /locus_tag="VIO83_01565"
     CDS             2365..2964
                     /locus_tag="VIO83_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEY8330006.1"
                     /translation="MINHVWGLFTHPDQEWQEIRGEHESISHMYLSHVLILAAIPAVS
                     AYIGTTQVGWSIGGGEAVRLTAGSALQMTILSYIAMLVGVAVMGAFIHWMARTYDANP
                     SLSDCMVFAAYCATPLFIGGLAALYPNMWLSMAVGTAAVCYTAYLLYVGIPSFMNIPK
                     DEGFMFSSSVLAVGLVVLVAIMATSVVLWGFGVGPVYAR"
     gene            3079..3570
                     /locus_tag="VIO83_01570"
     CDS             3079..3570
                     /locus_tag="VIO83_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEY8330007.1"
                     /translation="MPEQLMARVEACYQQAEAFFARRFLRPEVSLRLRGLKAGVAHLS
                     ENRLRFNAQLYRENREHFLRQTVAHEVAHLVAHQLFGSGIRPHGGEWQQVMAKVYGLP
                     AERCHSYAVKRRQRPYMYRCQCPDGEFTFTPQRHALVLRGRRYLCRRCRATLVFSGEQ
                     RLP"
     gene            complement(3545..4870)
                     /locus_tag="VIO83_01575"
     CDS             complement(3545..4870)
                     /locus_tag="VIO83_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEY8330008.1"
                     /translation="MPRQPFYKSLYVQVLVAIAIGVALGHFYPETGAAMKPLGDGFVK
                     LIKMAIAPIIFCTVVSGIAGMQDMKAVGKTGGIALLYFEIVSTVALVIGLVVVNLAQP
                     GAGMHIDPATLDASSIAAYTKAGAEQSTVAFLLNVIPNTVVGAFAEGNILQVLFFAIL
                     FAFALQRMGKLGKPVLEFIDGIAHVMFGIIGVIMKLAPLGAFGAMAFTIGKYGVGSLV
                     QLGQLMLCFYITCVFFVLVVLGAIARANGFSILRFVSYIREELLIVLGTSSSESALPR
                     MLNKMEKLGCHKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATDTPMDLTQQLTLL
                     AVLLISSKGAAGVTGSGFIVLAATLSAVGGLPVAGLALILGIDRFMSEARALTNLIGN
                     GVASVVVAKWVGQLDNAQLQRELAKPSRTPVELPLVAEPAELTAAAARR"
     gene            5188..5976
                     /locus_tag="VIO83_01580"
     CDS             5188..5976
                     /locus_tag="VIO83_01580"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY8330009.1"
                     /translation="MRSSLIGVLLAFSGLSHAAIQTQEIPYQSPDGTQLVGYYAWDDA
                     VSGKRPGVVVVHEWWGLNDYAKRRARDLAALGYSALAIDMYGEGRNTTHPADAKSFMQ
                     AATADADAARKRFLAGLDLLKQQPRTDKKKLAAIGYCFGGKVVLDMARQGVPLAGVVS
                     FHGPLATTTPATPGSVKARILVEHGAADSMVDAKQVDDFKAEMYKADIDYRFVSLPGA
                     KHGFSNPDADAYQAQGLDLGYQKAADERSWDDMQEFFKELFGQR"
     gene            complement(5951..6997)
                     /locus_tag="VIO83_01585"
     CDS             complement(5951..6997)
                     /locus_tag="VIO83_01585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY8330010.1"
                     /translation="MRLLIVSDAWAPQVNGVVTSLRALSTELQALGHEVCVLSPQDFR
                     TLPCPGYAAIPLAWDVWKVGGKIRAFAPDAVHLATEGPLGWAARRWLKREGLAFSTAI
                     HTRFPEYVSERWSWIPLAWGYAWLRAFHRPSQQVLVSTERLRDEFAQRDFPRLALWRK
                     GVDLQQFQPRLDTEEGAAPVFLYAGRIAPEKNLEAFLDLDLPGEKQVVGDGPLRAELE
                     RRYPQVRFFGYLDATGLAEAYRRASVLVFPSRTDTLGLVMLEALACGTPVAAFPVCGP
                     LDVLRQGVSGVMAEDLRQACLQALTLDREQCAAEAQRHSWPASALEFLACQPRLDGAP
                     CLPDALFSADRTAP"
     gene            complement(6994..7821)
                     /locus_tag="VIO83_01590"
     CDS             complement(6994..7821)
                     /locus_tag="VIO83_01590"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011268528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY8330011.1"
                     /translation="MTSVQALPPKGKQYIRTLWISDVHLGTRDCQAERLDAFLKRYAP
                     ERVYLVGDIIDGWKLRSGVYWPQSHTNVIRRLINMSRRGTEVIYVTGNHDECLRRYAD
                     LVLGNIRLVDEAEHLTADGRRLLVLHGDQFDVITRYHKWLAFLGDSAYEFTLTLNRWL
                     NHWRQRWGYGYWSLSAWLKHKVKGAVSYISEFEEALAHECTRRGFHGVVCGHIHHAEI
                     RSVGAIEYLNCGDWVESCSALIEHADGHIELYRLAADADAGVSVEDDPVEPLAEPSA"
     gene            complement(7902..8948)
                     /locus_tag="VIO83_01595"
     CDS             complement(7902..8948)
                     /locus_tag="VIO83_01595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY8330012.1"
                     /translation="MRLLIVSDTWEPRVSGVATCLRALVDELQALGHEVRVLSPQDFH
                     ALPCPGYPEIALVWDLWRVADKIRAFAPDAVHLAAEGPLGWVARCWLQREGLVFSTAI
                     HTRFAEFTQARWPLTPLSWGYAWLRVFHRPSAAVLVSTVRMQSEFARLGFAHLRLWRK
                     GVDLRQFRPRLEPQGEGGPVFLYVGRLAPEKNLEAFLDLDLPGDKQVVGDGPLRAELE
                     RRYPRVRFLGYRRGEALAEAYRRASVLVFPSRSDTLGLVMLEALACGTPVAAFPVAGP
                     LDVLRQGVSGVMDEDLRRACLQALSLDRHACLAEAQRQSWRASALEFLAHQPRLDGRP
                     CLSVKPHGVERSIP"
     gene            complement(9216..9509)
                     /locus_tag="VIO83_01600"
     CDS             complement(9216..9509)
                     /locus_tag="VIO83_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016781616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HEY8330013.1"
                     /translation="MSTPKPEIVITYCTQCQWLLRAAWLAQELLSTFSDDLGRVALEP
                     GTGGVFRITCDGVEIWERKADGGFPEAKVLKQRVRDRIDPQRDLGHSDRPRSD"
     gene            complement(9565..10962)
                     /locus_tag="VIO83_01605"
     CDS             complement(9565..10962)
                     /locus_tag="VIO83_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY8330014.1"
                     /translation="MGHDLDSLQARFGRHYPRWLLALLIVSTMTMVLSSTSINVALPA
                     IMADFAIGRPLAQWLVTGVLAAMTSGLLLSSWAQARFGPRATLLTSFGLFLLTSLVAP
                     FVTSIWALIAIRIVQGLCAGIAQPLSMVLIFRAYPGAGRGLALGLFGLGAMMAPALGP
                     TLAGFLVDHFGWHAVFWLPAPVCILSLLGGALLLPTLRSLTPPRLDVPGFILLNLALF
                     GILAALAEAQRFGWLHDATLVIGAVGLLGLAAFLWRCTHHPAPLLPLRLWRNASFRHT
                     SWVSMVLGVSLFGITYLIPLYVQTVEGYSASDAGLLLLPTGLVLGVAVFAGGWLSDRL
                     AARWLLVGGLGLLTLSTAGQALMGTGASFWAICAWASLGRLGIGGIMPGVSTGAVQDL
                     PAEELAPATGTISFMRQLGGALGINLLTFFLEWRHVAEGASAAGDAQAFVQSFWLLTV
                     LFALALWPAWLLRNR"
     gene            complement(10964..11395)
                     /locus_tag="VIO83_01610"
     CDS             complement(10964..11395)
                     /locus_tag="VIO83_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8330015.1"
                     /translation="MAHTDHRLGAQVAQLARTWRAELDRRLNHLGLSQARWLVLLHLA
                     RQPDAPTQSELAAMVGVECPTLARLLDGLEAQQLVRRVTVAEDRRAKRVELLPQTGEI
                     ITRIEAIADQLRSEILANVDQQELEVFQRVLGQMQANLERR"
     gene            complement(11555..>12159)
                     /locus_tag="VIO83_01615"
     CDS             complement(11555..>12159)
                     /locus_tag="VIO83_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEY8330016.1"
                     /translation="AQRSSGGPSQASQLVRGDERPLWEALRTLRRKLAEEHSVPPYVI
                     FPDATLLEMLRSQPQSLSDMARVSGVGARKLERYGQAFLDVLTDSPAAPAAPVADLRH
                     ELVSLARSGMTPQQIARQLDCSEKNVYSLLAEAIARQQLSLEQALDLPEELLGEIHDA
                     FLDGDAELPPVAAIAEAFDGRVALGVLHCVRAALESELGA"
BASE COUNT         2133 a         4136 c         4077 g         1813 t
ORIGIN      
        1 atggcgttgc gatcagtctg gatacgggcg gcgagcgtgt cgctcagcgg caggcgctgc
       61 cgcaacaggg cggggctggg cagcggctgg gcgctgcggc gggccgggga ttggggggtg
      121 gcgagtcggg cggaagcgga gacattcatg atctggcggt cctttggccg gcggcaatgc
      181 cgcgcggcgc tagcgaattg ttcggtcgag aggtagcgaa gcggcggtgc aacggctaaa
      241 tcgccagtcg tagcggcagt agcggtaaca ggtgcggtag ctgttcatcg gtggatcctc
      301 gttgcgtcgg gccttgtccg gccggaaaaa caaaaccccc ggttgggttg ccaaccgggg
      361 gtttggggga gctgctgggt ggcgaccctg ttactcgggc gcctgtgggg tatcaggcgc
      421 tcaggcagct aaaccaatac ccataaaaag aatacgcgcc agcgacgacc agcggcgcgc
      481 acgccagcgc ggcgcaagcg gcaccggtgc gggcgagggg ctgatggtcg aggctgagcg
      541 gcatgtcgtt ctccagaagg atgctggaac cttactgcag gggtggccgg ctgacaagcc
      601 ggccgcatcc actcagaggc tcatcacgtc gatgaagcgc ggggtagcat cgtcgtcgat
      661 cttcaggctc tggaagtcga acaggttgcg gtcggccagc tgcgagggca ccacgttctg
      721 cagggcgcgg aacatgatct cagtacgccc cggcgacttg cgttcccact cgtggagcat
      781 ctccttgacc acctgacgct gcaggttctc ctgcgagccg cacaggttgc acgggatgat
      841 cgggaactcc ttgagctggg cataagcctc gatgtccttc tcgctgcagt aggccagcgg
      901 acggatcacc acgttgcggc cgtcgtcgga gagcagcttg ggcggcatgg ccttgagggt
      961 gccgccatag aacatgttga ggaagaaggt ctcgaggatg tcgtcgcgat ggtggccgag
     1021 agccatcttg gtcgcaccga tctcgtcggc ataggtgtac agggtgccgc ggcgcaggcg
     1081 cgagcacagc gagcaggtag tcttgccttc cgggatcttc tccttgacca ccgagtaggt
     1141 gtccttctcg atgatgtggt agtcgacgcc gatggacttc aggtactccg gcagcacgtg
     1201 ctcggggaag cccggctgct tctggtccat gttcaccgcc acgatctcga acttgatcgg
     1261 cgcgaccttc tgcagataga gcagcacgtc gagcatggtg tagctgtcct tgccgccgga
     1321 caggcagacc atgaccttgt cgccgtcctc gatcatgttg aagtcggtga cggcttcgcc
     1381 ggccaggcga cgcaggcgtt tctgcagctt gttctggttg accgaaaggg tgcccatggt
     1441 ggtgtggagt ccgggggagt tgcgaaaaag gcgctcattt tacgcgcaaa cgcgcgatca
     1501 ccccagccca atcgagacca ccgatcgcgg atgtcggcgc ggcgctgcga ggcggatcac
     1561 gccctgcgcc gcagcccggc aatcgccaca cgggcgcatg ctaggctcac cccatgaaca
     1621 gccttttcga tcccgacctc gagcttgtcg agcaactgct gacgctgctg cgccaatccc
     1681 ccgacggcct cagcgaacac cagctcatcc atctgctcaa gagcggacac tgcacgcaca
     1741 tccccaatct gccgctgcgc gacaggctgg tgctgtttcg cacccatttc ctggtgttca
     1801 acgccctcta ccgtctacgc gaccgcctgt gggccgagca aagcgcgcac ctggaaatca
     1861 accccctgct gatccgcctg ctgccctatc gggatggcgc gcagggcgtc gtcgagcacg
     1921 atgcgctgcg cgcctactac ctcgacgaga cgcacctgcg cgacaccagc gaagcggatg
     1981 tcgaacacct gctgcagagt ttctggaccc gcatgcacca cggcgaggag cagcaggcgg
     2041 cgctggagct gttcggcctg gacgcgcgca ccgactaccc ggccatccgc ctgcgctatc
     2101 gccagctggt cagccagcac cacccggatc gcggcggcag caccacacgc ctgcagtcga
     2161 tcaacaaggc aatggaaatt ctggatcgct attacaaccc tcgctgagag gcgaaatcgc
     2221 tctaccctcc cccgctgcag ccggcgacca gcacctatac tgcgacataa ggtcgcatag
     2281 gccgacccgg caccggacgg cactggccac gtgcctccgg cgcctcgtct ggggcgatcc
     2341 gcatataaga aggaggtatt gctcatgatc aaccacgttt gggggctctt cacccatcct
     2401 gatcaggaat ggcaagagat tcgtggcgag cacgaaagca tcagccacat gtatttgagc
     2461 catgtcctga tactggctgc cataccggcc gtttccgcct acatcggcac cacccaggtc
     2521 ggctggtcga tcggcggcgg cgaggcggta cgcctgactg ccggcagcgc actgcagatg
     2581 accatactgt cctatatcgc catgctggtc ggtgttgcgg tgatgggtgc cttcatccac
     2641 tggatggccc gcacctacga tgccaacccc agcctgagcg actgcatggt gtttgctgcc
     2701 tactgcgcca cgcccctgtt catcggcggc ctggccgccc tgtatccgaa catgtggctg
     2761 agcatggcgg tcggcacggc agcggtgtgt tacacggcct acctgctgta tgtcggcatc
     2821 ccatccttca tgaacattcc caaggatgaa ggcttcatgt tctccagctc ggtgctggcg
     2881 gtgggactgg tggtcctggt ggcgatcatg gccacctcgg tcgtcctgtg gggatttggc
     2941 gtgggtcccg tctatgcccg ttgaataagc acggaccctt cacttgaggc cgcctgcggg
     3001 cggccttgtt gtttgtgccg cgcacgctgc cggccagttt gggcataatc ggcgccacac
     3061 ttccggaacc gcccgctcat gcccgaacag ctcatggccc gcgtcgaggc ctgctaccag
     3121 caggccgaag cctttttcgc ccgccgcttc ctgcgccccg aggtcagcct gcgcctgcgc
     3181 ggcctcaagg ccggggtcgc ccacctgagc gagaaccgcc tgcgcttcaa tgcccagctg
     3241 taccgggaaa accgcgagca tttcctgcgc cagaccgtag cccacgaggt ggcacatctg
     3301 gtcgcccacc agctgttcgg tagcggcatt cgcccgcacg gcggcgagtg gcaacaggtc
     3361 atggccaagg tctacggcct gccggccgag cgttgccatt cctacgcagt gaagcgccgg
     3421 cagcggcctt atatgtaccg ctgccagtgc ccagatgggg aatttacctt caccccgcag
     3481 cgccatgcgc tggtgctgcg ggggcgccgt tacctgtgcc ggcgctgcag ggcgacgctg
     3541 gtgttcagcg gcgagcagcg gctgccgtga gttcggccgg ctcggccacc agcggcagct
     3601 ccacgggcgt gcggctcggc ttggccagct cgcgctgcag ttgcgcgttg tccagctgac
     3661 cgacccactt ggccaccacc acgctggcca cgccgttgcc gatcaggttg gtcagcgcgc
     3721 gcgcctcgga catgaagcgg tcgatgccga ggatcagcgc cagaccggcc accggcaggc
     3781 caccgacggc ggacagggtg gcagccagca cgatgaagcc actgccggtc accccagcgg
     3841 cgcccttgga agagatcagc agcaccgcca gcagggtcag ctgctgggtc aggtccatcg
     3901 gcgtatcggt ggcctgggcg atgaacaccg cggccatggt caggtagatc gaggtgccgt
     3961 cgaggttgaa ggagtagccg gtcgggatca ccaggccgac caccgacttg tggcaaccga
     4021 gcttctccat cttgttcagc atgcgcggca gcgccgactc tgaggaagag gtgccgagca
     4081 cgatcagcag ctcctcacgg atgtagctga cgaagcgcag gatgctgaag ccgttggcgc
     4141 gggcgatggc gccgagcacc accagcacga agaacacgca ggtgatgtag aagcacagca
     4201 tcagctggcc gagctgcacc agcgaaccga cgccgtactt gccgatggtg aaggccatgg
     4261 cgccgaaggc cccgagcggc gccagcttca tgatcacgcc gatgatgccg aacatcacat
     4321 gggcgatgcc gtcaatgaac tccagcaccg gcttgccgag cttgcccatg cgctgcaggg
     4381 cgaaggcgaa caggatggcg aagaacagca cctggaggat gttgccctcg gcgaaggcgc
     4441 cgaccacggt gttcgggatc acgttcagca ggaaggccac ggtgctctgc tcggcaccgg
     4501 ccttggtata ggcggcgatg ctgctggcat ccagagtggc gggatcgata tgcatgccgg
     4561 cgcccggctg cgccaggttg accaccacca gaccgatgac cagcgccacc gtggagacga
     4621 tctcgaaata cagcagggca atgccaccgg tcttgcccac cgccttcatg tcctgcatgc
     4681 cggcgatgcc gctgaccacg gtacagaaga tgatcggcgc aatggccatc ttgatcagct
     4741 tgacgaagcc gtcgcccagc ggcttcatcg ccgcgccggt ttccggatag aaatgaccca
     4801 gcgcaacgcc gatggcgatc gcgaccagca cctggacgta caggctcttg tagaacggtt
     4861 gacgaggcat gactgttttt ctcccgggcg accgtcttcc agctccttgc ggcagacttc
     4921 accaagcgcg gtagccccct atccggggga cttgttgtac ccctcgtcca ggacgcaggg
     4981 gcagatggat acagcaagcc ccgtgccagc ctcgaaaaaa cccagaaaac cctctttaat
     5041 caatgacttg accaattcgc aaggcttcca gccgccctcc tccggcggga atccgccgcc
     5101 cacccggcgg ggaatccgcc agccttgcct tcacagccgt gattcggccg cctacgcttg
     5161 tgagtcagtc aacccggagg acttgtcatg cgttccagcc tgatcggcgt attgcttgcg
     5221 ttcagcggcc tgagccacgc cgccatccag acccaggaaa tcccctacca gagtcccgac
     5281 ggcacgcagc tggtcggcta ctacgcctgg gacgacgctg tttccggcaa acgccccggg
     5341 gtggtggtgg tgcacgagtg gtgggggctc aacgactacg ccaagcgccg cgcccgcgac
     5401 ctcgcggccc tcggctacag cgccctggcc atcgacatgt atggcgaagg caggaacacc
     5461 acgcacccgg ccgacgccaa gagtttcatg caggcggcga ctgccgacgc cgatgccgcc
     5521 cgcaagcgct tcctcgccgg cctcgacctg ctcaagcagc agccgcggac cgacaagaag
     5581 aagctggcgg ccatcggcta ctgcttcggc ggcaaggtgg tgctggacat ggcccgccag
     5641 ggcgtgccgc tggccggcgt ggtgagcttc cacggccccc tcgccaccac caccccggca
     5701 acccccggct cggtgaaggc ccggatcctg gtggagcacg gcgccgccga tagcatggtc
     5761 gacgccaagc aggtcgatga cttcaaggca gaaatgtaca aggccgacat cgactaccgc
     5821 tttgtcagcc tgcccggcgc caagcacggt ttcagcaatc ccgacgccga cgcctaccag
     5881 gcccagggcc tcgatctcgg ctaccagaag gccgccgacg agcgctcctg ggatgacatg
     5941 caggagttct tcaaggagct gttcggtcag cgctgaacag cgcatccggc agacagggcg
     6001 cgccatccag ccgcggctgg cacgccagga actccagcgc cgaggctggc cacgagtggc
     6061 gctgcgcctc cgcggcacac tgctcccggt ccagggtcag ggcttgcagg caggcctggc
     6121 gcaggtcctc ggccataacg ccgctgacac cctggcgcag cacgtccagc ggcccgcaga
     6181 ccggaaacgc cgccaccggc gtgccgcagg ccagcgcctc gagcatcacc agaccgaggg
     6241 tgtcggtgcg cgaggggaaa accagcacgc tggcgcgccg ataggcctcg gccagcccgg
     6301 tggcatccag gtagccgaag aagcgcacct gcggatagcg ccgctccagc tccgcgcgca
     6361 gcggcccgtc acccaccacc tgcttctcgc ctggcagatc gagatcgagg aacgcctcga
     6421 ggttcttctc cggtgcgatc cgcccggcat acagaaacac cggcgccgcc ccctcctcgg
     6481 tgtcgagacg cggctggaac tgctgcagat ccacgccctt gcgccacagc gccagacggg
     6541 ggaagtcgcg ctgggcgaac tcgtcgcgca gccgctcggt actgaccagc acctgctggc
     6601 tgggtcggtg gaaggcccgc agccaggcat agccccaggc cagcggaatc cacgaccagc
     6661 gctcgctcac atactcggga aagcgcgtgt ggatcgccgt ggaaaacgcc agcccctcgc
     6721 gcttgagcca gcgccgcgcg gcccagccga gcggcccttc ggtggccagg tgcacggcat
     6781 ccggggcaaa cgcccgaatc ttcccgccga ccttccagac atcccaggcc agcgggatcg
     6841 ccgcatagcc cggacagggc agcgtgcgga agtcctgcgg cgacagcacg cacacctcgt
     6901 ggccgagcgc ctgcagctcc gtgctcagcg cccgcaggct ggtgaccacg ccgttgacct
     6961 gcggcgccca ggcatcgctg acgatcagca gcctcatgcg cttggctccg ccaggggctc
     7021 gaccgggtca tcttccaccg acacccccgc gtcggcatcg gcggccaggc ggtacagctc
     7081 gatgtggccg tcggcatgct cgatcagcgc cgagcaggac tccacccagt cgccgcagtt
     7141 gaggtactcg atggcgccta cgctacggat ctcggcatgg tggatgtggc cgcagaccac
     7201 gccgtggaag ccgcgcctgg tgcactcgtg ggccagcgcc tcctcgaact cgctgatgta
     7261 gctgaccgcg cccttgacct tgtgcttgag ccaggccgac agcgaccagt agccgtagcc
     7321 ccagcgctgg cgccagtggt tgagccaacg attgagcgtg agggtgaact cgtaggccga
     7381 atcgccgagg aaggccagcc acttgtggta gcgggtgatc acgtcgaact ggtcgccgtg
     7441 cagcaccagc aggcggcggc cgtcggcggt cagatgctcg gcctcgtcga ccaggcggat
     7501 attgcccagc accaggtcgg catagcggcg caggcattcg tcgtggttgc cggtgacgta
     7561 gatcacctcg gtgccgcgcc gactcatgtt gatcagccgg cggatgacgt tggtatggct
     7621 ctgcggccag tacacgccgc tgcgcagctt ccagccatcg atgatgtcgc ccaccaggta
     7681 gacgcgctcg ggggcataac gcttgaggaa ggcgtccagg cgctcggcct ggcagtcgcg
     7741 ggtccccagg tgcacgtcgg agatccacag ggtacggatg tactgcttgc ccttgggcgg
     7801 gagcgcttgg acgctggtca tcgacggact ccgttttttc cgatcttcgg cggcgtcgat
     7861 gaaggcggcg tgacagcgcc gcggcagtgc ggtgacagga ctcagggaat gctgcgttcg
     7921 accccgtgcg gcttgaccga caggcagggc ctgccgtcca gccgcggctg atgggccagg
     7981 aactccagcg ccgaggcccg ccacgactgg cgctgcgcct cggcgagaca ggcgtggcgg
     8041 tccaggctca aagcctgcag gcaggcgcga cgcaggtcct cgtccatcac cccgctgacg
     8101 ccctggcgca gcacgtccag cggcccggcc acgggaaagg cggccaccgg cgtgccgcag
     8161 gccagcgcct cgagcatcac caggccgagg gtgtcgctac gcgaggggaa caccagcacg
     8221 ctggcacggc ggtaggcctc ggccagggcc tcgccgcgcc ggtagccgag aaagcgcacc
     8281 cgtggatagc gccgctccag ctccgcgcgc agtgggccgt cgccgaccac ctgcttgtcg
     8341 cccggcaggt cgagatcgag gaacgcttcg agattctttt ccggggccag gcggccgaca
     8401 tagagaaata ccggcccgcc ctccccctgc ggctcgaggc gcggccgaaa ctggcgcagg
     8461 tccacgccct tgcgccacag ccgcagatgg gcgaaaccga gccgggcgaa ctcgctctgc
     8521 atgcgcacgg tgctgaccag caccgcggcg ctcggccgat ggaacacccg caaccaggca
     8581 tagccccagc tcagcggcgt cagcggccag cgcgcctggg tgaactcggc aaaacgcgtg
     8641 tggatggcgg tggaaaacac cagcccctca cgctgcagcc agcagcgcgc cacccagcca
     8701 agcggacctt cggccgccag gtgcacggca tccggggcga acgcgcggat cttgtcggcc
     8761 actcgccaga gatcccagac cagggcgatt tccgggtagc ccggacaggg cagcgcatgg
     8821 aaatcctgcg gcgacagcac gcgcacctca tggccgagcg cctgcagctc gtccaccagc
     8881 gcgcgcaggc aggtggccac gccactgacc cgcggctccc aggtatcgct gacgatcagc
     8941 agccgcatgc ctgcgcctcg gcccgccagg aggcgaagcg gctggcaagc cggaaacggc
     9001 acggctgcgg gctgttacgc gacgggcacg gcggggcgct gtccatcgac aatctccggc
     9061 aggctggagc ggtagtcgca aaccgcgcag accagcagca ttggccactg ccgcacacgc
     9121 agactacagc atggttaccg cagcatagcc cagctcaggc aagagccctg ccggcagcgg
     9181 cccgccgaat cggtgccgaa cagcgccggg gcgactcagt cgctgcgcgg ccggtcgctg
     9241 tgtccgaggt cgcgctgcgg atcgatgcgg tcgcgcaccc gctgcttgag caccttggcc
     9301 tcggggaaac cgccgtcggc cttgcgctcc cagatctcca cgccgtcgca ggtgatgcgg
     9361 aacaccccgc cggtgccggg ctccagcgcc acccggccga ggtcgtcgct gaaggtggac
     9421 agcagttcct gggccagcca ggccgcgcgc agcagccatt ggcactgggt gcagtaggtg
     9481 atgacgattt cgggcttggg ggtggacatg ccggagacat cctcaacgtg atacgggcgg
     9541 gacgccatga tagcgcgccg cgcgtcagcg attgcgcagc agccaggccg gccagagggc
     9601 cagggcgaac agtacggtca gcagccagaa gctctgcacg aaggcctggg catcccccgc
     9661 ggcgctcgcc ccttcggcga catggcgcca ctcgaggaag aaggtcagca ggttgatgcc
     9721 cagcgccccg ccgagctgac gcatgaagct gatggtgccg gtggccggcg ccagctcctc
     9781 cgcgggcagg tcctgcaccg cgccggtact cacccccggc atgatgccgc cgatgccgag
     9841 gcgacccagc gaggcccagg cgcagatcgc ccagaagctc gcgccggtcc ccatcagcgc
     9901 ctgcccggcg gtggacaggg tgagcagacc gaggccgccg accagcagcc agcgcgcggc
     9961 cagcctgtcg ctcagccagc cgccggcgaa cacggcgaca cccagcacca ggccggtggg
    10021 cagcaacagc aggccggcgt cgctggcgct gtagccctcc accgtctgca cgtacagcgg
    10081 gatcaggtag gtgatgccga acagactcac tcccagcacc atcgacaccc agctggtatg
    10141 gcggaagctg gcattgcgcc acaggcgcag tggcagcagc ggcgcggggt ggtgggtgca
    10201 gcgccagagg aaggcggcca ggccgagcag gccgaccgcg ccgatcacca aggtggcgtc
    10261 gtgcagccag ccgaagcgct gcgcctcggc cagcgcggcg aggatgccga acagcgccag
    10321 gttgagcagg atgaagccgg gcacgtcgag gcgcggcggc gtcagcgagc gcagggtggg
    10381 cagcagcagc gcaccgccga gcaggctgag gatgcacact ggcgccggca gccagaacac
    10441 cgcgtgccag ccgaaatggt cgaccaggaa gccggccagg gtcggcccca gggccggcgc
    10501 catcatcgcc cccaggccga acaggccgag ggccaggccg cgccccgcgc ccggataggc
    10561 gcggaagatc agcaccatgg acagcggctg ggcgataccg gcgcacagcc cctggacgat
    10621 gcggatggcg atcagcgccc agatcgaggt gacgaagggc gccaccagcg aggtgagcag
    10681 gaacaggccg aagctggtca gcagggtcgc ccgcggaccg aagcgcgcct gcgcccagga
    10741 cgacagcagc aggccggagg tcatcgcggc gagcacgccg gtgaccagcc actgggccag
    10801 cggccggccg atggcgaagt ccgccatgat cgccggcagc gccacgttga tgctggtgga
    10861 cgacagcacc atggtcatgg tcgagacgat cagcagggcc agcagccagc gcgggtaatg
    10921 cctgccgaag cgggcctgca ggctgtcgag gtcgtggccc atgtcagcgc cgctccaggt
    10981 tcgcctgcat ctggcccagc acgcgctgga acacctccag ctcctgctgg tcgacgttgg
    11041 caaggatctc gctgcgcagc tggtcggcga tggcctcgat gcgggtgatg atctccccgg
    11101 tctgcggcag cagctcgacg cgcttggccc gccggtcctc ggccaccgtc acccggcgca
    11161 ccagttgctg cgcctcgaga ccgtcgagca ggcgcgccag ggtcggacac tcgacaccga
    11221 ccatggcggc cagctcgctc tgcgtcggcg cgtccggctg ccgcgccagg tgcagaagca
    11281 ccagccagcg cgcctgcgac agccccaggt ggttgagccg gcgatccagc tcggcacgcc
    11341 aggtgcgcgc cagctgggcg acctgggcac ccaggcggtg atcggtatgg gccatggaca
    11401 agcctccgga taatcaatga gctaattatt agctgactaa acattagctc gcaacctacc
    11461 tagccgaaga gcgccgaaag gaaggatgac agggcgctgc ggggcagcgc cggctcgcgt
    11521 gctcgcgagc caaggtcagg ccatgagcgc gccctcaggc gcccagctcg gactccagcg
    11581 ccgcccgcac gcaatgcagc acgccgagcg ccacgcggcc gtcgaacgcc tcggcaatcg
    11641 ccgctaccgg cggcagttcg gcgtcgccgt cgaggaaggc atcgtggatc tcgccgagta
    11701 gttcctcggg cagatccagc gcctgctcga ggctgagctg ctggcgagcg atggcttcgg
    11761 ccagcaggct gtagacgttc ttctcgctgc agtcgagctg gcgggcgatc tgctgcgggg
    11821 tcatcccgga gcgggccagg ctgaccagct cgtggcgcag gtcggccacc ggcgcggccg
    11881 gcgccgccgg gctgtcggtg agcacgtcga gaaaggcctg gccgtagcgc tccagcttgc
    11941 gcgcgccgac gccgctgacc cgcgccatgt cggacagcga ctgcggctgg ctgcgcagca
    12001 tctccagcag ggtggcgtcg gggaagatca cgtagggcgg cacgctgtgc tcctcggcca
    12061 gcttgcggcg cagggtgcgc agcgcctccc acagcggccg ctcgtcgccg cgcaccagct
    12121 ggctggcctg gctcggcccg ccggaactgc gctgggcac
//
LOCUS       DATCUN010000048         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_114795, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000048 DATCUN010000000
VERSION     DATCUN010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114795"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /gene="metG"
                     /locus_tag="VIO83_01620"
     CDS             complement(<1..1700)
                     /gene="metG"
                     /locus_tag="VIO83_01620"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY8330017.1"
                     /translation="MTRTALVTTALPYANGPLHLGHLVGYIQADIWVRARRMSGGKTW
                     FVCADDTHGTPIMLAAEKAGVTPEAFIANIQASHERDFAAFGVAFDHYDSTNSAANKA
                     LTEAFYLKLEAEGHIARRSVAQFYDPAKGMFLPDRYIKGICPVCSSPDQYGDNCEVCG
                     ATYAPTELKDPRSVISGATPEMRDSEHFFFEVGHFDAFLREWLAGDVALPGVKAKLGE
                     WLNADGGLRAWDISRDAPYFGFEIPGQPGKYFYVWLDAPIGYLSSFKTLCDKNGEDFD
                     AHLRAGTSTELHHFIGKDIVNFHGLFWPAVLHGTGHRAPTRLHVNGYLMVDGAKMSKS
                     RGTFVMARTYLDVGLEAEALRYYFAAKSGGGVEDLDLNLSDFTARVNADLVGKFVNLA
                     SRCAGFISKRFDGRLADSLPDAAQYDRFVAALAPIREAYERNDPAAAVRLTMTLADEA
                     NRYIDDQKPWVIAKQEGADAQLQAVCTQGLNLFRVLVTALKPILPATAAQADAFLAAP
                     VQDWTDIARPLTAHTIAVYAPLFTRIDPKMIDAMTDASKDTLAAAAPAAPAKPAKPAA
                     A"
     gene            complement(2387..>2597)
                     /locus_tag="VIO83_01625"
     CDS             complement(2387..>2597)
                     /locus_tag="VIO83_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEY8330018.1"
                     /translation="ANKNKPVKGMTILWNLKADGANAWSGGTILDPANGKTYKSKVKL
                     QTGGAKLDVSGCIAFICRSQTWVRE"
BASE COUNT          410 a          733 c         1007 g          447 t
ORIGIN      
        1 gcggcagcgg gcttggcggg cttggccggg gcagccgggg cggcagcggc gagggtgtcc
       61 ttggaggcgt cggtcatggc gtcgatcatt ttcgggtcaa tccgggtgaa cagcggggca
      121 tacacggcaa tggtgtgcgc ggtgagcggg cgggcgatgt cggtccagtc ctgcacgggg
      181 gcggccagga acgcgtcggc ctgggcggcg gtggccggca ggatcggctt gagcgcggtg
      241 accagcacgc ggaacaggtt caggccctgg gtgcagacgg cctgcagctg cgcgtcggcg
      301 ccctcctgct tggcgatcac ccacggcttc tggtcgtcga tgtagcggtt ggcttcgtcg
      361 gccagggtca tggtcaggcg cacggcggcg gccgggtcgt tgcgctcgta cgcctcgcgg
      421 atgggggcga gggcggcgac gaagcggtcg tactgggcgg catcgggcag gctgtcggcc
      481 aggcggccgt cgaagcgctt gctgatgaag ccggcgcagc ggctggccag gttgacgaac
      541 ttgcccacca ggtcggcgtt gacgcgtgcg gtgaagtcgc tcaggttcag atcgaggtct
      601 tcgacgccgc cgccggactt ggcggcgaag tagtagcgca gggcctcggc ttccaggccg
      661 acgtcgaggt aggtgcgggc catgacgaat gtgccgcgcg acttggacat cttggcgccg
      721 tcgaccatca ggtagccgtt gacgtgcagg cgggtgggcg cgcggtggcc ggtgccgtgc
      781 aggacggccg gccagaacag gccgtggaag ttgacgatgt ccttgccgat gaaatggtgc
      841 agttcggtgc tggtgccggc gcgcaggtgg gcgtcgaagt cttcgccgtt cttgtcgcac
      901 agggtcttga agctggacag gtagccgatc ggggcatcca gccagacgta gaaatacttg
      961 ccgggttggc cggggatttc aaagccgaag tagggggcat cgcgggagat gtcccaggcg
     1021 cgcaggccgc cgtcggcatt gagccattcg ccgagcttgg ccttgacgcc gggcagggcg
     1081 acgtcgccgg cgagccactc gcgcaggaag gcgtcgaagt ggccgacttc gaagaagaag
     1141 tgttcggagt cgcgcatttc cggggtggcc ccggagatga ccgagcgcgg gtctttcagt
     1201 tcggtggggg cgtaggtggc gccgcacact tcgcagttgt cgccgtactg gtccgggctg
     1261 ctgcagaccg ggcagatgcc cttgatgtag cggtcgggca ggaacatgcc tttggcagga
     1321 tcgtagaact gggccacgga gcggcgcgcg atgtggcctt cggcctcaag cttgaggtag
     1381 aaggcttcgg tgagcgcttt gttggcggcg gagttggtcg agtcgtaatg atcgaacgcg
     1441 acgccgaagg cggcgaagtc gcgctcgtgg ctggcctgga tgttggcgat gaaggcttcc
     1501 ggggtgacgc cggccttttc ggcggcgagc atgatggggg tgccgtgggt gtcatcggcg
     1561 cagacgaacc aggttttgcc gccgctcatt cgacgcgcac gcacccagat gtccgcctgg
     1621 atgtagccga ccaggtggcc caggtgcagg gggccgttgg cgtagggcag tgcggtggtg
     1681 actagagcgg tgcgggtcat ggtctgcgtt gttgccttgg ggaccgggga ttatcgcatg
     1741 gagggggtgg gcgcctgcgg cgggcttttt tttcgaagcc ggagccaggg ccagggccag
     1801 agccggagcc acagccacag ccacagccac tgccgaagcc actgccgaag ccacagccga
     1861 agcctgttga ccggtctgct gggggagggc tggggtgggt gaggccgcgg ggcacgctgc
     1921 aagtacgtcc gtgtaagctc cgccaccgca tccatgcggt ggagggcccc gcaacctcac
     1981 ccaccccagc ccttccacag ttggctggtg tgcatgggaa cggcgggggg caggggcaga
     2041 ggccgaagac taagcgaagc gaagcgaacc gggccgatca aaccgaaccg aaccgaaccg
     2101 aaaggagctt ctgcgtgccg gagccggaga tagagcggtt tggttcgggg tgtgggtggg
     2161 agttgaaatg caaaaggccg aagcggcgca gccgcttcgg ccttcttctt cctcccaacc
     2221 ggcgatgcac atgcctggag ggcgaggcgg gttcaacccc accgggaccg tccacggcat
     2281 ggatgccgtg gacgagcccc cagggatggg ttcacggcgt gtcccggtgg ggttgaaccc
     2341 gcctcgacca caccgcagca atgactagct aaaccaacgc cgaaatttac tcccgcaccc
     2401 atgtctggga gcggcagatg aaggcgatgc agccggagac gtcgagcttg gcgccaccgg
     2461 tctggagctt gaccttggac ttgtaggtct tgccgttggc cgggtccagg atggtgccgc
     2521 cggaccaggc gttggcgccg tcggccttca ggttccacag gatggtcatg cccttgaccg
     2581 gcttgttctt gttggcg
//
LOCUS       DATCUN010000049        10352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_264904, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000049 DATCUN010000000
VERSION     DATCUN010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10352
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264904"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1561)
                     /gene="gdhA"
                     /locus_tag="VIO83_01630"
     CDS             complement(224..1561)
                     /gene="gdhA"
                     /locus_tag="VIO83_01630"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEY8330019.1"
                     /translation="MSQSLDAFLTQLKQRDPNQPEFHQAVEEVVRSLWPFLQSEPRYM
                     KAAILERMVEPERAILFRVPWVDDQGVVRVNRGYRVQMSSAIGPYKGGLRFHPSVNLG
                     VLKFLAFEQVFKNSLTSLPMGGGKGGSDFDPKGKSDNEVMRFCQSFMTELYRHVGANL
                     DVPAGDIGVGGREIGFLFGQYKRLANEFTSVLTGKGMSYGGSLIRPEATGYGCVYFAQ
                     EMLKTRHEGFDGKRVAISGSGNVAQYAAQKVMELGGKVISLSDSAGTVLIEAGLDKEQ
                     FDYLMDLKNVRRGRIEEMAAHFGLQYLEGQRPWNLACDIALPCATQNELDADDARTLL
                     KNGCFCVAEGANMPSTLAAVDLFIEAGILYAPGKASNAGGVAVSGLEMSQNSMRLLWT
                     DGEVDAKLHSIMQNIHHACVSYGEENGRINYVKGANIAGFVKVADAMLAQGVV"
     gene            complement(1928..3271)
                     /locus_tag="VIO83_01635"
     CDS             complement(1928..3271)
                     /locus_tag="VIO83_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8330020.1"
                     /translation="MAQRALIVDDEPDIRELLEITLGRMKLDTRCARNVKEAREWLAR
                     EPFDLCLTDMRLPDGTGLELVQLIQQRYPQTPVAMITAYSSLDTAISALKAGAFDFLT
                     KPVDLARLRELVATALRLRSPQDESDGSVDSRLLGDSPPMRALRKQIAKLSRSQAPVY
                     ISGESGSGKELVARLIHELGPRTEQPFVPVNCGAIPSELMESEFFGHKKGSFTGAVED
                     KPGLFKAADGGTLFLDEVADLPLPMQVKLLRAIQEKAVRAVGGQQELAVDVRILSATH
                     KDLASEVTAGRFRQDLYYRLNVIELNVPPLRERREDIALLAERALQRLAVDCGMPPAR
                     LSSEALEKLKSYRFPGNVRELENMLERAYTLCEDDQIEAGDLRLGDAPAQGGGDGASG
                     NLAQIDDLEDFLENIERQAIMQALEETRWNRTAAAQRLGLSFRSMRYRLKKLGID"
     gene            complement(3274..4869)
                     /locus_tag="VIO83_01640"
     CDS             complement(3274..4869)
                     /locus_tag="VIO83_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8330021.1"
                     /translation="MNSADFWSPGTAQGQRILRLYHLYRLSVGLALVLLITSDMHREL
                     LDMVDARLFRHASWAYLATNVVLGVLVQQANRPLTLFGLALFDVTLLSTLFYAAGGLG
                     SGLAPLLVISVAMSNSILRGRLGLLIAALASSGLIYLTFYISLSRPAAAGYYVQAGAL
                     GALCFVAALIIQALSRRLQQSETLARRHAADVADLQVLNALILQRMRTGILALDPQHR
                     VLLANASSQSLLGQYPLENQQLEQCCPALLERLQQWQENPALRPPTLQAFAEGPVLQP
                     SFVPLRRKGERDILIFLEDVSKLAQQAQQLKLASLGRLTAGIAHEIRNPLGAISHAAQ
                     LLQEAEDLSAPDRRLTQIILDHSRRMNLVVENILQLSRRRQAEPQLLDLRYWLHRFAG
                     ELRETQRGDSTIHLQTEAGSIQTRIDPQQLTQVLTNLVQNGLRYSARKHGQGQVWLNL
                     FRDSDTQLPVLEIRDDGDGVPADQQGNLFEPFFTTENKGTGLGLYISRELCESNRARL
                     DYAPREGSGACFRITFAHPKTTS"
     gene            complement(4856..5089)
                     /locus_tag="VIO83_01645"
     CDS             complement(4856..5089)
                     /locus_tag="VIO83_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEY8330022.1"
                     /translation="MARLLTLIALFAIGWWLWRRFVRPPQDTRPPAAPKTQPMVRCAH
                     CGVHVPQNEALAHDNRWYCSRAHLEQDDSPEQR"
     gene            5275..6909
                     /locus_tag="VIO83_01650"
     CDS             5275..6909
                     /locus_tag="VIO83_01650"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019334670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY8330023.1"
                     /translation="MSQVLRVAMAQLNLRVGDIHGNVERIIEAAGRAREQWQADVIVF
                     PELSLCGYPPEDLLLRDSMQLRIEAALERLCREVRGIRLLLGFPWVEGERRYNACALL
                     DDGVLVARYHKQKLPNYQVFDEKRYFDAGDQACVVDIAGVPVALSICEDIWHAEPMAA
                     AHAAGARLMLNLNASPFHVGKQLQREETLAARAGEGGMPIVYVNQVGGQDELVFDGGS
                     CVVAASGHVLQRAPAYEEGLFLAELQVDEELVQPQPGACAVLADEEASVYDALVLGVR
                     DYVRKNGFKGVVLGLSGGIDSALTLAVAVDALGADKVEAVMMPYRYTAQISLEDAEAE
                     ARTLNVAYSVLPIEPMVEAFLGTLAPVFAGLGRDTTEENLQARCRGTLLMAISNKKGY
                     LVLTTGNKSEVAVGYCTLYGDMAGGFDVLKDVPKTLVFRLCEYRNSLGGEVIPQRVID
                     RPPSAELAPDQKDEDSLAPYPVLDEILRLYVEEDLSADAIIARGFAADTVRKILRLVD
                     LNEYKRRQAAIGPRITQRGFGRDRRYPITSGWRLGE"
     gene            complement(7050..8066)
                     /locus_tag="VIO83_01655"
     CDS             complement(7050..8066)
                     /locus_tag="VIO83_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY8330024.1"
                     /translation="MQVKHLLLIATLALTAACSSKQVIDENLSETELYTQAQEHLDNK
                     SYTSAVDTLKALESRYPFGSYAEQAQLELIYAYYQNQEPEAARAAADRFIRLHPQHPD
                     ADYAYYLKGLASFDKDRGLMARFLPLDMTKRDPGAARDSFNEFAQLVNRYPNSRYAPD
                     AKARMIYLRNLLAAYEIHVANYYLKRQAYVAAANRGRYVVENFQETPAVGDGLAVMTE
                     AYQRLGLDDLSASSLATLKLNYPEHPTLESGEFVPREEEADTRSWLSKATLGLIDNEA
                     PLPPGQTRTSQDMQRQYQEAVDAIPEELQPENAEETEAEEIEEDADAPKRSWWNRLTF
                     GLFD"
     gene            8213..9175
                     /gene="rluD"
                     /locus_tag="VIO83_01660"
     CDS             8213..9175
                     /gene="rluD"
                     /locus_tag="VIO83_01660"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEY8330025.1"
                     /translation="MSEIIQLRAEVPSELGGQRLDQVAAQVFPDHSRSRLAGWIKDGR
                     LTVDGAVLRPRDTVYGGAVLQLEAERDAQVEYLAQDIELNIVYEDEHILVLDKPAGLV
                     VHPAAGHADGTLLNALLHHAPELASVPRGGIVHRLDKDTTGLMVVAKTLEAHTNLVAQ
                     LQARSVSRIYEAIVLGVVTAGGKVDAPIGRHSQQRQKMAVSSTGKPAVSHYRVLERFR
                     AHTHVRVKLETGRTHQIRVHMAHVNFPLVGDPVYGGRFRIPPAANPVLVQALRDYPRQ
                     ALHARFLELDHPVTGERMGWESALPDDFVELLNLLRGDREAFIG"
     gene            9258..10055
                     /gene="pgeF"
                     /locus_tag="VIO83_01665"
     CDS             9258..10055
                     /gene="pgeF"
                     /locus_tag="VIO83_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY8330026.1"
                     /translation="MSIPKPISTQHGRRAADREQALGRGDLLIPDWPAPANVRACVTT
                     RAGGVSQVPFDSFNLGDHVGDDPVAVAENRRRLQALLGCRAAWLSQVHGVSVVEADPA
                     QVAEADAGWSATPGVACAIMTADCLPVLFCDRAGSHVAAAHAGWRGLAAGVLEASVAA
                     LDCPADQVLVWLGPAIGPQAFEVGAEVRAAFVEQHAEAAEAFVPSVNAGRFMADLYRL
                     ARIRLAAIGVTAVYGGGLCTFSDAARFYSYRREPRTGRLASLIWLER"
BASE COUNT         1590 a         3217 c         3640 g         1905 t
ORIGIN      
        1 ggtgtgaaag agttccatga ttatcctcgt catgctggct gagcttgttg gcggtcgtcg
       61 tccgcgcctt ccatggataa aaatatacgc tcattgagcg catatcgcaa gccccatttc
      121 tacacgattc cccgaatacc cataaaaaac gcaggcaaag aaaaagccgg cacagtggcc
      181 ggcttgttct gtactgctca ggcagtgtcg cgaggcagga gcctcagacc acgccttggg
      241 ccagcatggc gtcggcaacc ttgacgaagc cggcgatgtt ggcgcccttg acgtagttga
      301 tgcggccatt ctcctcgcca tagctgacgc aggcgtggtg gatgttctgc atgatgctgt
      361 gcagcttggc gtccacttcg ccgtcggtcc acagcaggcg catggagttc tggctcattt
      421 ccagacccga cacggccacg ccgccggcgt tggaggcctt gcccggggcg tagaggatgc
      481 cggcttcgat gaacaggtcg accgccgcga gggtggacgg catgttggcg ccttcggcca
      541 cgcagaaaca gccgttcttc agcagggtgc gtgcgtcgtc ggcgtccagt tcgttctggg
      601 tggcgcacgg cagggcgatg tcgcacgcca ggttccacgg acgctggcct tccagatact
      661 gcaggccgaa gtgagcagcc atctcttcga tgcgaccgcg acggacgttc ttcaggtcca
      721 tcaggtagtc gaactgttcc ttgtccaggc ccgcctcgat cagcacggtg ccggcagagt
      781 cggacaggga gatgaccttg ccgcccagct ccatgacctt ctgcgcggcg tactgggcca
      841 cgttgcccga gccggagatt gccacgcgct tgccgtcgaa gccctcgtgg cgggtcttga
      901 gcatttcctg ggcgaagtac acgcagccgt agccggtcgc ttccggacgg atcaggctgc
      961 cgccgtagct catgcccttg ccggtcagca ccgaggtgaa ctcgttggcc aggcgcttgt
     1021 actgaccgaa caggaagccg atttcacgac caccgacgcc aatgtcaccg gccggtacgt
     1081 cgaggttggc gccgacatga cggtacagct cggtcatgaa cgactggcag aagcgcatca
     1141 cttcgttgtc gctcttgccc ttcggatcga agtcggaacc gcccttgccg ccgcccatgg
     1201 gcagggaggt cagcgagttc ttgaacacct gctcgaaggc gaggaacttc agcacgccca
     1261 ggttgaccga cgggtggaag cgcaggccgc ccttgtacgg gccgatggca ctgctcatct
     1321 gcacgcggta gccacggttg acgcggacca cgccctgatc gtcgacccac ggtacgcgga
     1381 acaggatggc gcgttccggc tcgaccatgc gctcgaggat ggcggccttc atgtagcgcg
     1441 gctcgctttg caggaagggc cacaggctgc ggaccacctc ttccacggct tggtgaaatt
     1501 cgggctggtt ggggtcgcgc tgcttgagct gcgtcaggaa ggcgtcaaga gactgggaca
     1561 tggagaggcc tcggaagttg gctacgaata aatctggccg tgttcggcag ggggtgaaat
     1621 ttagcagggc atgcgcgcct cttcatagtg caaaatgacg cctttatgaa tctttttggt
     1681 gcctttttgt aagtgctggc cagaatgatg tttttttagg ctaatttcgt ctgttgagtg
     1741 cactgttttg gttatatatg cgcgctttgt tgtcttatct tggtgcgtac cggtttttcg
     1801 acctcctgcc cacaggcggg cgaatttttc ctggatcaag gcggggcctt cgccgtcggt
     1861 ggtgccgagc gaattcgtct gccagagcgt gccggaagcc ccggacggca gtgccgactg
     1921 ggggatgtca gtcgatgccg agctttttca gccggtagcg catggagcgg aatgacaggc
     1981 cgagccgctg ggcggcggcg gtgcgattcc agcgggtttc ctcgagggcc tgcatgatcg
     2041 cctgccgctc gatgttctcg aggaagtcct cgaggtcgtc gatctgcgcc aggttgccgc
     2101 tggcgccgtc accgccgccc tgcgccggtg catcgccgag gcgcaggtcg ccggcctcga
     2161 tctggtcgtc ctcgcacagg gtgtaggcgc gctcgagcat gttttccagc tcgcgcacgt
     2221 tgccggggaa gcggtagctc ttcagctttt ccagcgcctc gctgctcagg cgtgccggcg
     2281 gcatgccgca gtcgacggcc aggcgctgca acgcgcgctc ggcgaggagg gcgatgtctt
     2341 cgcggcgttc gcgcagcggc ggtacgttca gttcgatgac gttgaggcgg tagtagaggt
     2401 cctggcggaa acgcccggcg gttacttcgc tggccagatc cttgtgggtg gcgctgagga
     2461 tgcgcacgtc gacggcgagc tcctgctggc cgccgaccgc gcgcacggcc ttctcctgga
     2521 tcgcgcgcag cagcttgacc tgcatgggca gcggcaggtc ggccacctcg tctaggaaca
     2581 acgtgccgcc gtcggcggcc ttgaacagcc ccggcttgtc ttccaccgcg ccggtgaagc
     2641 tgcctttctt gtggccgaag aactcgcttt ccatcagctc ggaagggatg gcgccgcagt
     2701 tgaccggcac gaacggctgc tcggtgcgcg ggccgagctc gtggatcagc cgtgcgacca
     2761 gctccttgcc actgcccgat tcgccgctga tgtacaccgg tgcctggctg cgcgacagct
     2821 tggcgatctg cttgcgcagg gcgcgcatcg gcggcgagtc gccgagcagg cggctgtcca
     2881 ccgagccatc gctttcgtcc tgggggctgc gcaggcgcag ggcggtggcg accagctcgc
     2941 gcaggcgggc aaggtccacc ggtttggtga ggaagtcgaa ggcgccggcc ttgagggcac
     3001 tgatcgcggt gtccaggctg ctgtaggccg tgatcatcgc caccggggtc tgcggatagc
     3061 gctgctggat cagttgcacc agttccagac cggtaccgtc gggcaggcgc atgtcggtca
     3121 ggcacaggtc gaacggttcg cgcgccagcc actcgcgggc ttccttgacg ttgcgcgcgc
     3181 agcgggtgtc cagcttcatg cggccgaggg tgatttccag cagctcgcgg atatcgggtt
     3241 cgtcgtcgac gatcagggct cgttgcgcca tggtcagctc gtggtcttcg gatgggcgaa
     3301 ggtgatgcga aagcaggcgc cgctgccttc gcgaggcgcg tagtccaggc gggcacggtt
     3361 gctttcacac aactcgcggg agatgtacag gccgaggccg gtgcccttgt tttcggtggt
     3421 gaagaacggc tcgaacaggt tgccctgctg gtcggcggga acgccgtcgc cgtcgtcgcg
     3481 gatttccagc accggcagct gcgtgtcgct gtcgcggaac aggttcagcc agacctggcc
     3541 ctggccgtgc ttgcgggcgc tgtagcgcag gccgttctgc accaggttgg tcagcacctg
     3601 ggtcagctgc tgggggtcga tgcgggtctg gatcgagccg gcttcggtct gcaggtggat
     3661 cgtcgagtca ccgcgctggg tttcgcgcag ctcgccggca aagcggtgca gccagtagcg
     3721 caggtcgagc agctgcggtt cggcctggcg ccgccgcgac agctgcagga tgttttccac
     3781 caccagattc atccgtcgcg aatggtcgag aatgatctgg gtcaggcggc ggtccggtgc
     3841 gctcaggtcc tcggcctcct gcagcagttg ggcggcgtgg ctgatggcgc ccagcgggtt
     3901 gcggatctcg tgggcgatgc cggcggtcag gcggcccagg gaggccagct tgagctgctg
     3961 cgcctgctgg gcgagtttcg agacatcctc gagaaagatc aggatgtcgc gctcgccctt
     4021 gcgccgcagc gggacgaagc tgggctgcag caccggccct tcggcgaagg cctgcagggt
     4081 tggcgggcgc agcgccgggt tttcctgcca ttgctgcagg cgctcgagca gcgctggaca
     4141 gcattgttcg agctgctggt tttccagtgg gtactggccg agcagggact gactgctggc
     4201 gttggccagc aacacgcgat gctgcgggtc gagggccagg atgccggtgc gcatgcgttg
     4261 caggatcagc gcgttgagga cctgcaggtc ggccacgtcg gcggcgtgcc ggcgggccag
     4321 ggtttcgctc tgctgcaggc gccggctcag cgcctggatg atcagcgcgg caacgaagca
     4381 cagcgcgccg agagccccgg cctggacgta gtagccggcg gctgccggcc ggctgaggct
     4441 gatgtagaac gtcaggtaga tcagcccgct gctggccagc gccgcgatca gcaggcccag
     4501 gcgcccgcgc aggatgctgt tggacatcgc caccgaaatc accagcagcg gcgccaggcc
     4561 gctgccgagg ccgccggcgg cgtagaacag ggtgctgagc agggtcacgt cgaacagggc
     4621 cagcccgaac agggtcagtg gccggttggc ctgttgcacc agcacgccga gcaccacgtt
     4681 ggtggccagg taggcccagc tggcgtggcg gaacaggcgg gcatcgacca tgtccagcag
     4741 ttcacggtgc atgtcgctgg tgatcagcag gaccagggcc aggccgacgc tgaggcgata
     4801 gagatggtac aggcgcagga tgcgctgccc ctgtgcggtg cccggcgacc agaagtcagc
     4861 gctgttcagg ggaatcgtct tgttccaggt gggcacggct gcaataccag cggttgtcgt
     4921 gggcgagagc ttcgttctgc ggcacatgca cgccgcagtg ggcgcagcgc accatcggct
     4981 gggtcttggg cgcggcgggg gggcgggtgt cctgtggcgg ccggacaaag cgtcgccaca
     5041 gccaccagcc gatggcgaac agagcgatga gggtcaacag gcgagccata atgctctcgt
     5101 gcgaggcggg aaagggcgcc agtgtagcca agccggcgtc acgcgcccag caccgtcgcc
     5161 cacgactgtg cgttgtgtcg gctgtggccg ttgcgggaga atgcggctcg tgcatcggcg
     5221 atgcgggcgg ctccggtggc tgtcggcagc cgcttcttcc agatggagag cagaatgagt
     5281 caagtcctgc gggtcgcgat ggcccagctc aaccttcgcg tcggcgatat tcacggcaac
     5341 gtcgagcgca tcatcgaggc ggccggacgc gcccgcgaac agtggcaggc ggatgtgatc
     5401 gtgtttcccg agctgtcgct gtgcggttat ccgccggagg acctgctgct gcgtgacagc
     5461 atgcagctgc gcatcgaggc ggcgctcgag cggctgtgtc gcgaggtacg tggcatccgc
     5521 ctgctgctcg gctttccctg ggtggagggc gagcggcgct acaacgcctg tgcgctgctc
     5581 gacgacggcg tgctggtggc ccgctaccac aagcagaagc tgcccaatta ccaggtgttc
     5641 gacgagaagc gctacttcga tgccggcgac caggcctgcg tggtggacat cgccggcgta
     5701 ccggtggcgc tgagcatctg cgaggacatc tggcatgccg agccgatggc cgccgcgcat
     5761 gccgccggcg cgcggctgat gctgaacctc aatgcctcgc cgttccacgt gggcaagcag
     5821 ctgcagcgtg aggaaaccct ggcggcgagg gcgggcgagg gcggcatgcc gatcgtctac
     5881 gtcaaccagg ttggcggcca ggacgagctg gtgttcgacg gcggctcctg tgtggtggct
     5941 gccagcggcc atgtgttgca gcgtgcaccg gcctatgagg agggcctgtt cctcgccgag
     6001 ctgcaggtgg acgaggagct ggtgcagccg cagcccggcg cctgcgcggt gctggccgat
     6061 gaggaggcca gcgtgtacga cgcgctggtg ctcggcgtgc gcgactatgt gcgcaagaac
     6121 ggcttcaagg gcgtggtgct tggcctgtcc ggtggcatcg actcggcgct gaccctggcg
     6181 gtggcggtgg atgcgctggg ggccgacaag gtcgaggccg tgatgatgcc gtaccgctac
     6241 accgcgcaga tcagcctgga ggatgccgag gccgaggcgc gcacgctgaa cgtggcctac
     6301 agcgtgctgc cgatcgaacc gatggttgag gcctttctcg gcaccctggc gccggtgttc
     6361 gccggcctgg gccgcgacac caccgaggag aacctgcagg cccgctgccg cggcacgctg
     6421 ctgatggcga tctccaacaa gaagggctac ctggtgctga ccaccggcaa caagagcgag
     6481 gtggcggtcg gctactgcac cctgtacggc gacatggccg gcggcttcga cgtgctcaag
     6541 gacgtgccca agaccctggt gttccgcctg tgcgagtacc gcaacagcct gggcggcgag
     6601 gtcattccgc agcgggtcat cgaccgtccg ccgtcggccg agctggcgcc cgaccagaag
     6661 gacgaggatt cgctggcgcc ctatccggtg ctggacgaga tcctccgcct gtacgtcgag
     6721 gaggacctgt cggccgacgc catcatcgcc cgcggcttcg ccgccgatac ggtgcgcaag
     6781 atcctgcgcc tggtcgatct caacgagtac aagcgccgcc aggccgccat cggcccgcgc
     6841 atcacccagc gcggcttcgg ccgcgaccgc cgctatccga tcacttcggg ctggcgcctc
     6901 ggcgagtgag gccgggcgta cgtcggcggg ctgggggcag gcagtaatcc accgcccgga
     6961 cggcagagca ctggcaacag tgctgccgtg ctgttgacgg acatgaaaaa gggaggccgc
     7021 agcctccctt tttcgttgcc ggtcgtgcct cagtcgaaca gaccgaaggt caggcgattc
     7081 caccaggaac gcttcggcgc gtcggcgtct tcctcgattt cttcggcctc ggtctcttcc
     7141 gcgttttccg gctgcagttc ctcggggatg gcgtccacgg cttcctggta ctggcgctgc
     7201 atgtcctggc tggtgcgggt ctggcccggc ggcagcggcg cttcgttgtc gatcaggccg
     7261 agggtggcct tgctcagcca ggagcgggta tcggcttctt cttctcgcgg cacgaactcg
     7321 ccgctttcca gggtcggatg ctcggggtag ttgagcttca gggtggccag gctgctggcc
     7381 gacaggtcgt ccaggccgag gcgctggtag gcttcggtca tcaccgccag tccgtcgccc
     7441 acggccgggg tttcctggaa gttttccacc acgtagcggc cgcggttggc ggcggcgacg
     7501 taggcctggc gcttcaggta gtagttcgcg acatggatct cgtaggcggc cagcaggttg
     7561 cgcagataga tcatgcgcgc cttggcatcg ggagcgtagc ggctgttggg gtagcggttg
     7621 accagttggg cgaactcgtt gaacgagtcg cgggcggcgc ccgggtcgcg cttggtcatg
     7681 tccagcggca ggaagcgcgc catcaggccg cggtccttgt cgaaggaggc cagaccttta
     7741 aggtagtagg cgtagtcggc gtcagggtgc tgcgggtgca ggcgaatgaa ccgatcggcg
     7801 gcggcacggg cggcctccgg ctcctggttc tggtagtagg cgtagatcag ttcgagctgc
     7861 gcctgctcgg cgtagctgcc gaacgggtag cgcgactcca gggccttgag tgtgtcgacg
     7921 gcgctggtgt agctcttgtt gtccaggtgc tcctgcgcct gcgtgtacag ctcggtctcg
     7981 ctgagattct cgtcgatgac ctgcttggac gagcaggccg cggtgagagc gagggtggcg
     8041 atcagcagca ggtgtttcac ttgcatggcg cttgggttcc ctggggccgg gtgcgtgaca
     8101 ggggccggcc tgatatgatt ggtgccccag aatcctctgg ggaaaattcg ccgtatttaa
     8161 ccacaagcct gtggctgaaa ccaaagccgc acggccctcc ccgatgtccg ccatgtccga
     8221 aatcattcaa ttgcgtgccg aggtgccgtc cgaactgggc ggacaacgtc ttgaccaggt
     8281 cgccgcccag gtctttcccg atcattcgcg ctcgcgcctg gctggctgga tcaaggatgg
     8341 ccggctgacc gtcgacggtg ccgtgctgcg tccgcgtgac accgtctatg gcggtgccgt
     8401 gctgcagttg gaggccgagc gcgacgcgca ggtcgagtac ctggcgcagg acatcgagct
     8461 gaacatcgtc tacgaggacg agcacatcct ggtgctggac aagccggccg ggctggtcgt
     8521 gcatccggct gccgggcatg ccgacggcac cctgctcaac gccttgctgc accatgcccc
     8581 cgagctggcc agcgtgccgc gcggcggcat cgtccaccgc ctcgacaagg acaccaccgg
     8641 cctgatggtg gtagccaaga cactcgaggc gcacaccaat ctggtcgccc agctgcaggc
     8701 gcgcagcgtc agccgcatct acgaggccat cgtcctcggc gtggttaccg ccggtggcaa
     8761 ggttgatgcg cccatcggcc gccactcgca gcagcgccag aaaatggcgg tgagctccac
     8821 cggcaagccg gcggtcagcc actaccgcgt gctcgagcgc ttccgtgcgc atactcatgt
     8881 gcgggtcaag ctggaaaccg ggcgcaccca ccagattcgc gtgcacatgg cccacgtcaa
     8941 tttcccgctg gtcggcgatc cggtctacgg cgggcgcttc cgcattccgc cggcggccaa
     9001 tccggtgctg gtccaggccc tgcgcgacta tccgcgccag gcgctgcatg cgcgcttcct
     9061 cgagctggac catccggtca ccggcgagcg catgggctgg gaatcggcgc tgccggacga
     9121 cttcgtcgaa ctgctcaacc tgctgcgcgg tgatcgcgag gcgtttatcg gatgagtcga
     9181 agagccggct cacgatctcg cgagctaagg tcaggcaagg cgaaatcggt tgaggacgcg
     9241 gagtttacga ggtgtaaatg agcattccga aaccgatttc aacgcagcat ggccgacgcg
     9301 cagcagatcg tgaacaggct ctgggccggg gcgacctgct gattcccgat tggcctgcac
     9361 cggccaacgt gcgcgcctgc gtgaccaccc gtgcgggcgg cgtcagccag gtgccgttcg
     9421 acagcttcaa tctcggcgac catgtcggtg acgatcccgt cgccgtggcg gagaatcgtc
     9481 gtcgcctgca ggcgctgctc ggctgccggg cggcctggct cagtcaggtg cacggcgtga
     9541 gcgtggtcga ggccgatccg gcacaggttg ccgaggccga tgccggctgg agtgccactc
     9601 ccggcgtggc ctgcgccatc atgaccgccg actgcctgcc agtgctattc tgcgaccgcg
     9661 ccggcagtca cgtggcagcg gcgcatgccg gctggcgcgg gctggctgcc ggcgtgctgg
     9721 aggccagcgt tgcggcgctg gattgtccgg ctgatcaggt gctggtctgg ctgggtccgg
     9781 ccattggccc gcaggccttc gaggtcggcg ccgaggtgcg tgctgccttc gtcgagcagc
     9841 atgccgaggc cgccgaggcc ttcgtgccca gcgtcaatgc cgggcggttc atggccgatc
     9901 tgtaccggct ggcgcgcatt cgcctggcgg ccatcggcgt caccgccgtg tacggtggcg
     9961 ggctgtgcac cttcagcgac gccgcgcgct tctattccta ccgccgcgaa ccgcgtaccg
    10021 ggcgcctggc cagcctgatc tggctggagc gttagcgccg taggagccag cttgctggcg
    10081 atcaacgccg accaaggatc gccagcaagc tggctcctac gggtctctgt ccgccggctt
    10141 gaccccggtc aagttcccgc cccttgaatc gcctgtaatc gtcctcatct atgctgcaac
    10201 cgaatcccta ggcggactgt tggtgttgtc gggtagcggc tgcccggtcc gctgttgctt
    10261 gcgaaggacg aacccatgcg tatcgaccgt ttgaccagca aactgcaact ggcgctttcc
    10321 gacgcccagt ccctggccgt gggccgcgat aa
//
LOCUS       DATCUN010000050         4636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_39783, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000050 DATCUN010000000
VERSION     DATCUN010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39783"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VIO83_01670"
     CDS             complement(<1..438)
                     /locus_tag="VIO83_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY8330027.1"
                     /translation="MTVPSPQPRRGRPPKVDRDNRETREALLHTGMRLLTEQGFLVTG
                     IDAVLKQVGVPKGSFYHYFASKEAFGRAVLERYAGYFAAKLDRWLLDESRPPLARLED
                     FVADARTGMARHGYRRGCLVGNLGQEVTQLPEGFREQLEAVLRD"
     gene            complement(538..2346)
                     /gene="oadA"
                     /locus_tag="VIO83_01675"
     CDS             complement(538..2346)
                     /gene="oadA"
                     /locus_tag="VIO83_01675"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HEY8330028.1"
                     /translation="MNKKITVTDTILRDAHQSLLATRMRLEDMLPICAKLDKVGYWSL
                     EVWGGATFDACVRFLKEDPWERLRQLRAALPNTRLQMLLRGQNLLGYRHYSDDVVKAF
                     VAKAAVNGIDVFRIFDAMNDVRNLRTAIEAVKAAGKHAQGTISYTTSPVHTVASFVEL
                     GKRMQEKGVDSIAIKDMAGLLTPYATAELVQALKAEIDLPIFIHSHDTAGMGAMCQLK
                     AIEAGADHIDTAISSFAWGTSHPGTESMVAALRGTPFDTGLDLELLQEIGLYFYGVRK
                     KYHQFESEFTSVDTRVQVNQVPGGMMSNLANQLKEQGALHRINEVFAEIPRVREDLGF
                     PPLVTPTSQIVGTQAVFNVLAGERYKTITNEVKLYLQGRYGRAPGKVNEHLRFQAIGS
                     EEVIDVRPADLLKPEMARLREEIGSLAKSEEDVLTFAMFPDIGRKFLEERAAGTLTPE
                     ALLPPPDAAARPAAQEGVPTEFVVDVHGESYRIDITGVSVKTDGKRHFYLAIDGMPEE
                     VVFEPLNAFVGGAASGKRKQTAAPGDVSTTMPGNIVDVLVKEGDVVKAGQPVLITEAM
                     KMETEVQAPIAGTVTAIHVVKGDRVNPGEVLIEIGS"
     gene            complement(2358..3773)
                     /locus_tag="VIO83_01680"
     CDS             complement(2358..3773)
                     /locus_tag="VIO83_01680"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY8330029.1"
                     /translation="MIRKILIANRGEIAVRIVRACAEMGIRSVAVYSEADRHALHVKR
                     ADEAYNLGAEPLAGYLNPRKLVNLAVETGCDALHPGYGFLSENAELADICAERGIKFI
                     GPSAEVIRRMGDKTEARRSMIKAGVPVTPGTEGNVADLDEALREGERIGYPVMLKATS
                     GGGGRGIRRCNSQEELEQAYPRVISEATKAFGSAEVFLEKCIVNPKHIEAQILADSFG
                     NTVHLFERDCSIQRRNQKLIEIAPSPQLTPEQRAYIGELAVKAAKAVGYENAGTVEFL
                     LAEGEVYFMEMNTRVQVEHTITEEITGIDIVREQIRIAAGLPLSVKQEDIIHRGYAIQ
                     FRINAEDPKNNFLPSFGKITRYYAPGGPGVRTDTAIYTGYTIPPYYDSMCLKLIVWAL
                     TWEEALDRGLRALDDMRVLGVKTTALYYQEILRNPEFRSGVFNTSFVEAHPELINYSV
                     KRKPEELALAIAAAIAAHAGL"
     gene            3933..>4636
                     /locus_tag="VIO83_01685"
     CDS             3933..>4636
                     /locus_tag="VIO83_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8330030.1"
                     /translation="MRKYLMRLTLRQLQVFRAVCETRSYSRAADEMALTQPAVSQQIR
                     QLEELLDQPLFEYVGKKLYLTDAAEALQRASADIFGRLESLDMQLSDLRGSLQGQLSL
                     TVESSAEYFVPHLFAAFRQQHPEVGLQLTVTSHAQALRRLGVSRDELMILSQVPTNMN
                     LEFNPFLTNHIVAVAPAEHPLCQHERLSLQELTAWPLLVREPGSGTRQACEAFCHDKR
                     AHFAQTLQLGSQEAQRA"
BASE COUNT          703 a         1484 c         1602 g          847 t
ORIGIN      
        1 gtcgcgcagc accgcttcca gctgttcgcg gaagccctcg ggtagctggg tcacttcctg
       61 gccgagattg ccgaccaggc agccgcgccg gtaaccgtgg cgggccatgc cggtacgggc
      121 gtcggcaacg aagtcctcca gacgcgccag cggcgggcgc gactcgtcga gcagccagcg
      181 atccagcttg gcggcgaagt agccggcgta gcgctccagc acggcgcggc cgaaagcctc
      241 cttgctggcg aagtagtggt agaaggagcc cttgggcacg ccgacctgct tgagcaccgc
      301 gtcgatgccg gtgaccagga agccctgctc ggtgagcagg cgcatgccgg tatgcagcag
      361 cgcctcgcgg gtttcgcggt tgtcgcggtc gaccttgggc gggcggccgc ggcgcggctg
      421 gggggatggg acagtcatgg gctgaatatt agaccgaccg tctagaatgt caatcgcgcg
      481 cgagggcgcg atcgaggaga ataaaaaagg ggagccttgc ggctcccccc ggggggctta
      541 acttccaatc tcgatgagta cttcaccggg attgaccctg tcgcccttaa ccacatggat
      601 ggccgtgacg gtgccggcga tcggcgcctg tacctcggtc tccatcttca ttgcttcggt
      661 gatcagtacc ggctggccgg ccttgaccac gtcgccttcc ttcaccagca cgtcgacgat
      721 gttgcccggc atggtggtgc tgacgtcgcc cggtgccgcg gtctgcttgc gcttgccgct
      781 ggcggcgccg ccgacgaagg cgttgagcgg ttcgaacacc acttcttccg gcatgccgtc
      841 gatggccagg tagaagtggc gcttgccgtc ggtcttgacg ctgacgccgg tgatgtcgat
      901 gcggtagctc tcgccgtgca cgtcgaccac gaactcggtc ggcacgcctt cctgggcggc
      961 cgggcgggcg gcggcatccg gcggcggcag cagtgcttcc ggagtcagag tgccggcggc
     1021 gcgttcctcg aggaacttgc ggccgatgtc ggggaacatg gcgaaggtca gcacgtcctc
     1081 ttcggacttg gccaggctgc cgatttcctc acgcaggcgg gccatttccg gcttgagcag
     1141 gtcggccgga cggacgtcga tgacttcctc gctgccgatg gcctggaagc gcaggtgctc
     1201 gttgaccttg cccggcgcgc ggccgtagcg gccctgcagg tacagcttca cctcgttggt
     1261 gatggtcttg tagcgctcgc cggccagcac gttgaacacc gcctgggtgc cgacgatctg
     1321 cgaggtcggg gtgaccagcg gggggaagcc gaggtcttcg cgcacgcgcg ggatttccgc
     1381 gaacacttcg ttgatgcggt gcagggcgcc ctgctccttc agctggttgg ccaggttgga
     1441 catcatgccg cccggcacct ggttgacctg cacgcgggtg tccaccgagg tgaactcgct
     1501 ctcgaactgg tggtacttct tgcgcacgcc gtagaagtac agaccgatct cctgcagcag
     1561 ctccagatcc aggccggtgt cgaacggagt gccacgcagg gcggcgacca tcgactcggt
     1621 gcccgggtgg ctggtgcccc aggcgaagct ggagatggcg gtgtcgatgt ggtcggcacc
     1681 ggcctcgatg gccttgagct ggcacatcgc gcccatgccg gcggtgtcgt gggagtggat
     1741 gaagatcggc aggtcgatct cggccttcag cgcctgcacc agttcggcgg tggcgtaggg
     1801 ggtcagcagg ccggccatgt ccttgatggc gatcgagtcg acgcccttct cctgcatgcg
     1861 cttgcccagc tcgacgaacg aggcgacggt gtgcaccggg ctggtggtgt agctgatggt
     1921 gccctgggcg tgcttgccgg cggccttgac cgcctcgatg gcggtgcgca ggttgcgcac
     1981 gtcgttcatc gcatcgaaga tgcggaacac gtcgatgccg ttgaccgcgg ccttggcgac
     2041 gaaggccttg accacgtcgt cgctgtagtg gcggtagccg agcaggttct ggccgcgcag
     2101 gagcatctgc aggcgggtgt tgggcagcgc ggcgcgcagc tggcgcaggc gctcccacgg
     2161 gtcttccttg aggaagcgca cgcaggcgtc gaaggtggcg ccgccccaga cttccagcga
     2221 ccagtagccg accttgtcga gcttggcgca gatcggcagc atgtcctcga ggcgcatgcg
     2281 ggtggccagc agcgactggt gcgcatcacg caggatggta tcggtgacgg tgatcttctt
     2341 gttcatctct caattcctca caggccggcg tgggcggcaa tggcggcggc gatggccagg
     2401 gccagctctt cgggcttgcg cttgaccgag tagttgatca gttccgggtg ggcttcgacg
     2461 aagctggtgt tgaacacgcc gctgcggaac tccggattgc ggaggatttc ctggtagtag
     2521 agcgcggtgg tcttcacgcc gagcacgcgc atgtcgtcca gcgcgcgcag gccgcggtcc
     2581 agtgcctctt cccaggtcag tgcccagacg atcagcttca ggcacatcga gtcgtagtac
     2641 ggcggaatgg tgtagccggt gtagatcgcc gtgtcggtgc gcacgccggg gccgccgggc
     2701 gcgtagtagc gggtgatctt gccgaaggac ggcaggaagt tgttcttcgg gtcctcggcg
     2761 ttgatgcgga actggatcgc atagccgcgg tggatgatgt cttcctgctt gaccgacagc
     2821 ggcaggcccg cggcgatgcg gatctgctcg cggacgatgt cgatcccggt gatttcctcg
     2881 gtgatggtgt gttccacctg cacccgggtg ttcatctcca tgaagtacac ctcgccctcg
     2941 gcgagcagga actccacggt accggcgttc tcgtagccga ccgccttggc cgccttgacc
     3001 gccagctcgc cgatgtaggc gcgctgctcg ggggtcagct gcgggctggg ggcgatctcg
     3061 atcagcttct ggttgcggcg ctggatcgag cagtcgcgct cgaacaggtg cacggtgttg
     3121 ccgaagctgt cggcgaggat ctgcgcctcg atgtgcttgg ggttgacgat gcacttctcg
     3181 aggaacacct cggcgctgcc gaaggccttg gtggcttcgg agatcacccg cgggtaggcc
     3241 tgctccagct cttcctgcga gttgcagcga cggatgccgc ggccgccgcc gccggaggtg
     3301 gccttgagca tcaccgggta gccgatgcgc tcgccttcgc gcagcgcctc gtcgaggtcg
     3361 gcgacgttgc cttcggtgcc cggagtgacc ggcacgccgg ccttgatcat gctgcgccgc
     3421 gcctcggtct tgtcgcccat gcggcgaatg acctcggccg acgggccgat gaacttgatc
     3481 ccgcgctcgg cgcagatatc cgccagttcg gcgttctcgg agaggaagcc gtagcccggg
     3541 tgcagggcgt cgcaaccggt ctcgacggcc aggttgacca gcttgcgcgg gttcaggtag
     3601 ccggccagcg gctcggcacc gaggttgtag gcttcgtcgg cgcgcttgac gtgcagcgcg
     3661 tggcggtcgg cttcggaata cacggccacc gagcggatgc ccatctcggc acaggcgcgc
     3721 acgattcgca cggcaatctc gccacggttg gcgatgagga tttttcttat cacggaggtc
     3781 tccctcggct gggctggctg accggccggt caggccgcag tacaaatacc ctagcgccga
     3841 gagatggatt actaaaaatg aataattatt gggttgagca taagtgctga cttatacttg
     3901 tgcgaaaagc ccgtcccaga ggccttcccg ctatgcgcaa gtacctgatg cgcctgaccc
     3961 tgcgccagct gcaggtgttc cgcgcggtgt gcgagacgcg ctcctacagc cgcgccgccg
     4021 acgaaatggc gctgacccag ccggcggtca gccagcagat ccgccagctc gaggagctgc
     4081 tcgaccagcc gttgttcgag tacgtcggca agaagctcta cctcaccgac gccgccgagg
     4141 ccctgcagcg cgccagcgcg gacatcttcg gccgcctgga aagcctcgac atgcagctct
     4201 ccgacctgcg cggctcgctg cagggccagc tcagcctgac cgtggaatcc agcgccgaat
     4261 acttcgtgcc gcacctgttc gccgcgtttc gccagcagca ccccgaagtc ggcctgcagc
     4321 tcaccgtcac cagccatgcc caggcgctgc gccgcctcgg cgtcagccgc gacgaactga
     4381 tgatcctctc ccaggtgccc accaacatga acctggagtt caatccgttc ctcaccaacc
     4441 acatcgtcgc cgtggccccg gccgagcacc cgctgtgcca gcacgagcgc ctgagcctgc
     4501 aggagctgac cgcctggccg ctgctggtgc gcgagccagg ctcgggcacg cgccaggcct
     4561 gcgaggcgtt ctgccacgac aagcgcgcgc acttcgccca gaccctgcag ctcggctcgc
     4621 aggaggcgca gcgcgc
//
LOCUS       DATCUN010000051         7099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_227311, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000051 DATCUN010000000
VERSION     DATCUN010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227311"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VIO83_01690"
     CDS             <1..318
                     /locus_tag="VIO83_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY8330031.1"
                     /translation="TQFKERIAHGMLTGALISAAIACTLPGPGTIYLGQNLRFTRPVK
                     LGDALTVELEVLEKLPKNRVRIATRVLNQQGKSVVDGEAEVMAPQDKLSIALTELPPI
                     SVG"
     gene            complement(448..567)
                     /gene="ccoM"
                     /locus_tag="VIO83_01695"
     CDS             complement(448..567)
                     /gene="ccoM"
                     /locus_tag="VIO83_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit CcoM"
                     /protein_id="HEY8330032.1"
                     /translation="MFMDEVVVAGIIIVVLTAAFLGGFGYFVWKDSQKGKKPH"
     gene            complement(833..991)
                     /locus_tag="VIO83_01700"
     CDS             complement(833..991)
                     /locus_tag="VIO83_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330033.1"
                     /translation="MSDDTLIDFAAERERRIHDLHEKRLQDVRQAFEQVLPLSARKKK
                     AKGKPKKR"
     gene            1192..4041
                     /gene="rapA"
                     /locus_tag="VIO83_01705"
     CDS             1192..4041
                     /gene="rapA"
                     /locus_tag="VIO83_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005732270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HEY8330034.1"
                     /translation="MAQYQPGQRWISDSEAELGLGTILAQDGRLLTVLYPATGDTRQY
                     ALRNAPLTRVRFSPGDEITHFEGWKLTVQEVEDVDGLLVYRGLNAQHESCTLPETQLS
                     NFIQFRLASDRLFAGQIDPMSWFALRYQSLEHASRLLQSPLWGLAGARAQPIAHQLHI
                     AREVADRASPRVLLADEVGLGKTIEAGLILHRQLLSGRARRALILVPENLQHQWLVEM
                     RRRFNLQVALFDAERFVESDADNPFEDCQLALVALDWLRDDVRAQQAVLEAEWDLLVV
                     DEAHHLVWHPEQASAEYRLVEELAANIPGVLLLTATPEQLGLESHFARLRLLDPDRFH
                     DLEAFRAESAQYQPVAAAVQELLDHGKLSAAARAAIHGFLGNEGDDLLANVEGGNEEA
                     RARLIRELLDRHGTGRVLLRNTRAAVQGFPERELHAYPLANPAEYMELPVGEHADLYP
                     EVGYQAQLGDDADDSQRWWRIDPRVEWLIDTLKMLKKVKVLVICAHAETAMDLEEGLR
                     VRAGTPATVFHEGMSILERDRAAAYFADEEFGAQVLICSEIGSEGRNFQFAHHLVLFD
                     LPAHPDLLEQRIGRLDRIGQQHTIQLHVPYLETSAQERLFQWYHQALNAFLNTCPTGN
                     ALQHQFGPRLLPLLEGGDDGEWSQLLGEGQAARESLEAELHSGRDRLLELNSGGAGEG
                     EALVEAILEQDDEFALPIYMEALFNAFGIDSEDHSENALILKPSEKMLDAGFPLGDDE
                     GVTVTYDRNQALVREDMQFLTWEHPMVQGGMDLVISGTMGNTSVALIKNKALKPGTVL
                     LELLYISEVVAPRALQLGRYLPPAALRCLLDANGNDLASRVSFETLHDQLESVPRASA
                     NKFVQAQRDVLAKLIGSGEGKIAPRHAERVAEASRRFAAELEEELARLEALRAVNPSV
                     RESELDSLRQRREQGQALLDKASLRLEAIRVLVAG"
     gene            complement(4237..5019)
                     /locus_tag="VIO83_01710"
     CDS             complement(4237..5019)
                     /locus_tag="VIO83_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY8330035.1"
                     /translation="MKLCANLSLLFSELPLLERIQAAADAGFAGVEIQFPYEVPAAEL
                     QDALQRAAMPLVLINLPAGDLMTGGPGLAAVPQRQGEFDTALQQALAYAAVVRPQCVN
                     VLPGRLAEGLDRAAALATLTANLRRAAEAFAPLGIRVLAEAINPIDMPGFLVNTPDHL
                     LALIAAVEHANFAAQLDFYHMARQDLDLPTCIAALAGHIGHVQFADCPGRGAPGTGAV
                     DFLAARQALAASGYVGWLGAEYRPGGEGTAASLGWLAEWRSW"
     gene            complement(5016..5906)
                     /locus_tag="VIO83_01715"
     CDS             complement(5016..5906)
                     /locus_tag="VIO83_01715"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8330036.1"
                     /translation="MSTLPSLAFAGIGLMGLPMSRRLLAAGFPLSVWNRNPAKCETLV
                     DAGAQVLVSPGDLCREADIVMLCLADTAAVREVVFGPGGIVEQGLPGQLLVDFSSCEP
                     AATREMAVELEERCGMRWVDAPVSGGTPGAEAGSLAIMAGGAEDDIERLRPILAHLGQ
                     RLTRMGEVGAGQVTKVCNQMIVACNALVIAEVVALAEQAGVDASLLAPALYGGFADSK
                     PLQILAPQMAESCFEPVKWHVRTLLKDLDTAVKLSREQGSATPMSGLAAQLMRLHGSQ
                     GNLERDPATLVELYRQEGEA"
     gene            6027..6878
                     /locus_tag="VIO83_01720"
     CDS             6027..6878
                     /locus_tag="VIO83_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8330037.1"
                     /translation="MLKRTLALAAAIALSVSALAAQAADVLKVSAIPDEAPTELQRKF
                     KPLGAYLEKELGMPVQFVPVSDYAAVVEGIAADRVDLAWLGGFTFVQTRLKTGNAIPL
                     VQREQDEKFTSKFISADANVKSLQDLKGKTFAFGSVSSTSGSLMPRYFMQKDGIVPEQ
                     FFSRVAFSGAHDATVAWVQAGKVDAGVLNASVWDKLVAAGKVDTAKVKVFATTPTYYD
                     YNWTVRGTLDPKLTAKIKAAFLALDPANPEHKAILDLQAASRFIETKPENYAGIEEAA
                     RAAGLLK"
     gene            6875..>7099
                     /locus_tag="VIO83_01725"
     CDS             6875..>7099
                     /locus_tag="VIO83_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8330038.1"
                     /translation="MSVRLTGVGMTHANGRRALQEVDLTVSRGERLAIIGPSGAGKTT
                     LLRLLATSLQPTEGRVELLGAEPWQLAAGAR"
BASE COUNT         1208 a         2559 c         2306 g         1026 t
ORIGIN      
        1 acccagttca aggagcgcat cgcccacggc atgctcaccg gtgcgctgat cagcgcggcc
       61 atcgcctgca ccctgccggg gccgggcacc atctacctcg gccagaacct gcgcttcacc
      121 cgtccggtga agctcggcga cgccctgacc gtggagctgg aagtactgga gaagctgccg
      181 aagaaccgcg tgcgcatcgc cacccgcgta ctcaaccagc agggcaagtc cgtggtggat
      241 ggcgaagccg aggtcatggc gccgcaggac aagctcagca tcgccctgac cgagctgccg
      301 ccgatcagcg tcggctgagg ccagcggcgg aaagcacaac gcccacgaaa tcgtgggcgt
      361 tgtgctttcc ggggcttttc tgcagacaaa aaaaacgggc gaccgaagtc gccccaattg
      421 ccttgcgtgc tcgtgagtcg ggaaggatca gtgcggcttt ttgcccttct gcgaatcctt
      481 ccagacgaaa tatccaaacc cacccagaaa cgccgctgtc agaacgacga tgatgatgcc
      541 tgcgactact acctcgtcca taaacatggc ttgtctccac gtgcgtcttt gctgctgttg
      601 aagccagaat acccatgccc gaccagggga aattgaccgg gatcaacagg cgccaacgcc
      661 caggtgggtg aaggcaggca aaccctgacg cagatcaacg gaagccgcag gccgggcgag
      721 cgccagcgct gcgcggcgag ggtgggaagg gggcaggacg ggaaaaccac ccgaccggca
      781 gccaggcgag agccatcggc gccaggccgt caggcgggag cgaatggtcg ggctagcgct
      841 ttttcggctt gcccttggcc ttcttctttc tggccgacag cggcagtacc tgctcgaagg
      901 cctggcgcac gtcctgcaga cgcttctcgt gcaggtcgtg gatgcgccgc tcgcgctcgg
      961 cggcaaagtc gatcagggtg tcgtcactca tggcggcgct ccttggcagc gggcgaaacg
     1021 ggcggaagtc gacgccccgg atatctggca atgatggggg cggatggcgc cagtgaccag
     1081 cccctgcgcg ggccaccccg acgatcgccg cgcaccggcg gcgcttgtct ttggcctgcc
     1141 tggcgacctt ccgctaacat aggcacttta ttttcgtgtc tggagtcatg catggcgcag
     1201 tatcaaccgg ggcaacgctg gatcagtgac agcgaggcgg aactggggct gggaaccatc
     1261 ctcgcgcagg acggccgcct gctcaccgtc ctctatcctg cgaccggcga tacccggcag
     1321 tacgcactgc gcaacgcacc gctgactcgc gtgcgcttct cgccgggcga cgagatcacc
     1381 cacttcgaag gctggaagct gaccgtgcag gaagtcgagg acgtcgacgg cctgctggtc
     1441 taccgcggcc tcaacgccca acacgaaagc tgcaccctgc cggaaaccca gctgtccaac
     1501 tttatccagt tccgcctggc cagcgaccgt ctgttcgccg ggcagatcga cccgatgagc
     1561 tggttcgccc tgcgctacca gagcctcgaa cacgccagcc gcctgctgca gtcgccgctg
     1621 tggggcctgg ccggcgcgcg cgcccagccg atcgcccacc agctgcacat cgcccgcgaa
     1681 gtggccgacc gcgccagccc ccgcgtgctg ctggccgacg aggtgggtct gggcaagacc
     1741 atcgaggccg gcctgatcct gcatcgccag ctgctcagcg gccgcgcccg ccgcgcactg
     1801 atcctggtcc cggaaaacct gcagcaccag tggctggtgg agatgcgccg gcgcttcaac
     1861 ctgcaggtcg ccctgttcga cgccgagcgc ttcgtcgaga gcgatgccga caacccgttc
     1921 gaggactgcc agctggcgct ggtcgccctc gactggctgc gtgacgacgt gcgcgcccag
     1981 caggccgtgc tggaggccga gtgggacctg ctggtggtcg acgaggcgca ccatctggtc
     2041 tggcacccgg agcaggccag cgccgaatac cggctggtcg aggagctggc ggccaacatc
     2101 cccggcgtcc tgctgctcac cgccaccccc gagcagctcg gcctggaaag ccatttcgcc
     2161 cgcctgcgcc tgctcgatcc cgaccgtttc cacgacctcg aggcctttcg cgccgaaagc
     2221 gcgcagtacc agcccgtggc ggctgcggtg caggagctgc tcgaccacgg caagctgtcc
     2281 gccgctgcgc gtgccgcgat tcacggcttc ctcggcaacg agggcgacga tctgctggcc
     2341 aacgtcgagg gcggcaacga ggaggcccgc gcacgcctga tccgcgagct gctcgaccgc
     2401 catggcaccg gccgcgtgct gctgcgcaac acccgcgccg ccgtgcaggg cttccccgag
     2461 cgcgagctgc atgcctaccc gctggccaac ccggcggagt acatggaact gccggtcggc
     2521 gagcatgccg acctctaccc ggaagtcggc taccaggcgc aactgggcga cgacgccgac
     2581 gacagccagc gctggtggcg catcgacccg cgcgtcgagt ggctgatcga caccctgaag
     2641 atgctcaaga aggtcaaggt gctggtgatc tgcgcccacg ccgagaccgc gatggacctg
     2701 gaggaaggcc tgcgcgtgcg cgccggcacc ccggccacgg tgttccacga gggcatgagc
     2761 atcctcgagc gcgaccgcgc cgccgcctac ttcgccgacg aggagttcgg cgcgcaggtg
     2821 ctgatctgct cggaaatcgg cagcgagggc cgcaacttcc agttcgccca ccatctggtg
     2881 ctgttcgacc tgccggccca cccggacctg ctcgagcagc gcatcggccg tctcgaccgc
     2941 atcggccagc agcacaccat ccagctgcac gtgccctacc tggaaaccag cgcccaggag
     3001 cgcctgttcc agtggtacca ccaggccctg aatgccttcc tcaatacctg cccgaccggc
     3061 aatgccctgc agcatcagtt cggcccgcgc ctgctgccgc tgctcgaagg cggcgacgac
     3121 ggcgagtgga gccagctgct gggcgagggc caggcggccc gcgaaagcct ggaggccgag
     3181 ctgcacagcg gccgtgaccg cctgctggag ctcaactccg gtggcgccgg cgagggcgag
     3241 gcgctggtcg aggcgatcct cgagcaggat gacgagttcg ccctgccgat ctacatggaa
     3301 gcgctgttca acgccttcgg catcgacagc gaggaccatt cggaaaacgc gctgatcctc
     3361 aagcccagcg agaagatgct cgacgccggc ttcccgctgg gcgacgacga gggcgtcacc
     3421 gtcacctacg accgcaacca ggcgctggtc cgcgaggaca tgcagttcct cacctgggag
     3481 cacccgatgg tgcagggcgg catggacctg gtgatttccg gcaccatggg caacacctcg
     3541 gtggcgctga tcaagaacaa ggcgctgaaa cccggcaccg tgctgctcga actgctctac
     3601 atcagcgaag tggtggcgcc gcgcgccctg cagctcggcc gctacctgcc gccggcggcg
     3661 ctgcgttgcc tgctcgacgc caacggcaac gacctcgcct cgcgcgtcag cttcgagacc
     3721 ctgcacgacc agctggaaag cgtgccgcgc gccagcgcca acaagttcgt ccaggcccag
     3781 cgcgacgtgc tggccaagct gatcggcagc ggcgaaggca agatcgcccc gcgccacgcc
     3841 gagcgcgtgg ccgaggccag ccgccgcttc gccgccgaac tcgaggaaga actggcacgc
     3901 ctggaagccc tgcgcgcggt caaccccagc gtgcgcgaga gcgagctgga cagcctgcgc
     3961 cagcgccgtg aacagggcca agccctgctg gacaaggcct cgctgcgtct ggaagcgatc
     4021 cgggtactgg tggccggcta aaccacgcca cgcccgcgcc gactcccgcg cgcccgcctg
     4081 ggagtcggcg ccgcaatgaa aaaggccgtg ccgggacacc ccgcacggcc ttttgctatt
     4141 catcgctttc agactacccg atggctccca cgccggagcg tgggaaccat cgggatcacg
     4201 ctttcccgta gggtgcgcca tgcgcaccct acacgctcac cacgagcgcc actcggccag
     4261 ccagcccagg ctggccgcgg tgccctcccc gcccggccgg tactcggcac ccagccagcc
     4321 gacgtagccg ctcgccgcca gcgcctgccg cgcagcgagg aaatccaccg cacccgttcc
     4381 cggcgcaccg cgccccggac aatcggcaaa ctgcacatgg ccgatgtgcc cggccagcgc
     4441 ggcgatgcag gtcggcaggt cgaggtcctg gcgggccatg tggtagaaat cgagctgggc
     4501 ggcaaagttg gcatgctcca ccgccgcgat cagcgccagc agatggtccg gcgtgttgac
     4561 caggaagccc ggcatgtcga tcgggttgat cgcctcggcc agcacgcgga tgcccagcgg
     4621 agcgaaagcc tcggcggcgc gacgcaggtt ggcggtcagg gtcgccagtg cagccgcgcg
     4681 gtcgaggccc tcggccagcc gccccggcag cacgttgacg cactgcgggc gcacaaccgc
     4741 cgcataggcc agcgcctgct gcagcgccgt gtcgaattca ccctggcgtt gcggcaccgc
     4801 cgccagtccc gggccgcccg tcatcaggtc gccggccggc aggttgatca gcaccagtgg
     4861 cattgccgcg cgctgcaggg cgtcctgcag ttcggcggcc ggcacttcgt agggaaactg
     4921 gatctccact ccggcgaaac cggcatccgc cgccgcctgg atgcgctcga gcagcggcag
     4981 ctcactgaac agcagactca ggttggcaca gagcttcatg cctcgccctc ctgacggtac
     5041 agctcgacca gggtggccgg atcgcgctca aggttgccct ggctaccgtg caggcgcatc
     5101 agctgggcgg ccaggccgct catcggcgtg gccgaaccct gctcgcggga cagcttgacc
     5161 gcggtatcga ggtccttgag caaggtacgc acgtgccact tgaccggctc gaaacagctc
     5221 tcggccatct gcggggcgag gatctgcagc ggcttggagt cggcgaagcc gccgtacagc
     5281 gccggcgcca gcaggctggc atccaccccg gcctgctcgg ccagcgccac cacctcggcg
     5341 atcaccaggg cgttgcaggc gacgatcatc tggttgcaca ccttggtcac ctggccggca
     5401 ccgacctcgc ccatgcgcgt caggcgctgg ccgagatggg cgaggatcgg ccgcagccgc
     5461 tcgatgtcgt cctccgcgcc gccggccatg attgccaggc tgccggcctc ggcgcccggg
     5521 gtgccgccgg acaccggcgc gtccacccag cgcatgccgc agcgctcctc cagctcgacg
     5581 gccatctcgc gggtggccgc cggctcgcag ctggagaagt ccaccagcag ctgacccggc
     5641 aggccctgct cgacgatgcc gccggggccg aacaccacct cgcgcaccgc cgcggtgtcg
     5701 gccaggcaga gcatgacgat gtcggcctcg cggcacaggt cgccggggct gaccagcacc
     5761 tgcgcgccgg catccaccag cgtctcgcac ttggccgggt tgcggttcca cacgctgagg
     5821 ggaaagccgg cggccagcag gcggcggctc atcggcaggc ccatcaggcc gataccggcg
     5881 aaggcaagag aaggcagggt cgacatcgcg agctccagtg ggtataaggt gccgccatct
     5941 taaccctagg gtcgccgtac caacatccga ccagcgcagc cctatactca cattcgcttt
     6001 ttccgctatt gaaccggaga actcccatgc tcaagcgcac cctggcgctc gccgccgcta
     6061 ttgccctgtc cgtttccgcc ctggccgcgc aggccgccga tgtcctcaag gtttccgcca
     6121 tccccgacga ggcgcccacc gagctgcagc gcaagttcaa gccgctgggt gcctacctgg
     6181 agaaggagct gggcatgccg gtacagttcg tcccggtctc cgactacgcc gccgtggtcg
     6241 agggcatcgc cgccgatcgc gtcgacctgg cctggctggg cggcttcacc ttcgtgcaga
     6301 cccgcctgaa gaccggcaac gccatcccgc tggtgcagcg cgagcaggac gagaagttca
     6361 ccagcaagtt catcagcgcc gacgccaacg tgaagtcgct gcaggacctc aagggcaaga
     6421 ccttcgcctt cggctcggtg tcctccacct ccggcagcct gatgccgcgc tacttcatgc
     6481 agaaggacgg catcgttccc gagcagttct tctcccgcgt ggccttctcc ggcgcccatg
     6541 acgccaccgt cgcctgggtg caggccggca aggtcgacgc cggtgtgctg aacgcctcgg
     6601 tgtgggacaa gctggtcgcc gcgggcaagg tcgacaccgc caaggtcaag gtgttcgcca
     6661 ccacgccgac ctactacgac tacaactgga ccgtgcgtgg caccctcgat cccaagctga
     6721 ctgccaagat caaggccgcc ttcctcgccc tcgatccggc caacccggag cacaaggcga
     6781 tcctcgacct gcaggccgcc agccgcttca tcgagaccaa gccggaaaac tacgccggca
     6841 tcgaggaagc cgcccgcgcc gccggcctgc tgaagtgagc gtgcgcctga ccggcgtggg
     6901 catgacccac gccaacggtc ggcgcgccct gcaggaagtc gatctgacgg tcagccgcgg
     6961 cgagcggctg gccatcatcg gcccgtccgg tgccggcaag accaccctgc tgcgcctgct
     7021 ggccaccagc ctgcaaccga ccgagggacg ggtggagctg ctcggtgccg agccctggca
     7081 actggccgcc ggtgcgcgc
//
LOCUS       DATCUN010000052         7719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_77300, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000052 DATCUN010000000
VERSION     DATCUN010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77300"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VIO83_01730"
     CDS             complement(<1..203)
                     /locus_tag="VIO83_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019409165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEY8330039.1"
                     /translation="MTTLNRDTHSKTIGYLLWLFGFLGAHRFYYGRPVTGTLWFCTLG
                     LLGIGWLIDLFLIPGMDRQADLR"
     gene            complement(284..1432)
                     /locus_tag="VIO83_01735"
     CDS             complement(284..1432)
                     /locus_tag="VIO83_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY8330040.1"
                     /translation="MEFEKLLRLMVEKGGSDLFITAGVPPSMKINGKILPVTKSPMAP
                     EQTRETVLSVMNEQQRRELAETHECNFAISARGIGRFRVSAFYQRNLIGMVLRRIETS
                     IPTFEDLNLPDVLKQLSMTKRGLVLFVGATGAGKSTSLAAMIGYRNKNSQGHIISIED
                     PIEFIHQHQGCIVTQREVGIDTESYDVALKNTLRQAPDVIMIGEVRTRETMDYAVAFA
                     ETGHLCLATLHANNANQALDRIIHFFPADRHEQVWMDLSLNLRAIVAQQLVPTPDGKG
                     RRAVIEVLINTPLMADLIRKGEVHELKPLMKRSAEQGMQTFDQALYQLYSNGEITYED
                     ALAHADSANDLRLMIKLGSETDAEHLGNLTQSLSLERSEEDQPGLRRR"
     gene            complement(1508..2542)
                     /locus_tag="VIO83_01740"
     CDS             complement(1508..2542)
                     /locus_tag="VIO83_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008019959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEY8330041.1"
                     /translation="MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMEH
                     KQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVARFRVNAFNQNRGAGAVFRTIPSR
                     VLTMEDLGMGEVFKKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
                     PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAA
                     ETGHLVFGTLHTTSAAKTIDRIVDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGR
                     VAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGALGMQTLDACLKTLVSKGLVTRDAA
                     KEKAKAPESL"
     gene            2614..3321
                     /locus_tag="VIO83_01745"
     CDS             2614..3321
                     /locus_tag="VIO83_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY8330042.1"
                     /translation="MSTIHDNIAKVRTRIREAAQASGRDPHSVQLLAVSKTKPAAALR
                     AAFACGQRDFGENYLQEALAKQAELADLDLAWHFIGPIQSNKTKPLAEHFAWVHSVDR
                     LKIAERLSAQRSPHLPPLNICLQVNVSGETSKSGCNPAELPALAAAVAALPNLRLRGL
                     MAIPEPTADVAAQRAAFARLRELKDSLPLPLDTLSMGMSDDLEAAIAEGATWVRIGSA
                     LFGARDYPQTSSARDAR"
     gene            3356..4174
                     /gene="proC"
                     /locus_tag="VIO83_01750"
     CDS             3356..4174
                     /gene="proC"
                     /locus_tag="VIO83_01750"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003142635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY8330043.1"
                     /translation="MNHPRIAFIGAGNMATSLIGGLIARGVPAERLTASEPQAEQRQR
                     IAREHGIAVQEDNAAAVAGAEIVVLAVKPQVMKDVCLALAPALPAGVLVVSIAAGIPC
                     ASLERWLGEQAIVRCMPNTPALLHEGASGLYANARTSAQQREQAEALLDAVGLVLWLE
                     EEQLIDAVTAVSGSGPAYFFLLMEAMTAAGERLGLPAETASLLARQTAFGAARMALVS
                     DFDPAELRRRVTSPNGTTEAAIKAFQAGGFEALVDQALHAAAHRSAEMAEQLGQ"
     gene            4184..4777
                     /locus_tag="VIO83_01755"
     CDS             4184..4777
                     /locus_tag="VIO83_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY8330044.1"
                     /translation="MNGLNTAAVYVLQTLGSLYLLIVLLRFILQLVRADFYNPLSQFA
                     VKATKPLLNPLRKVIPGFGGLDLASLVLALLVQLLLMIFTLLLMGAFDVALLPQLLIW
                     AVIGVTALFVKIFFFALIASVILSWVAPHSHNPGAQLVNQLCEPLLMPIRRILPNLGG
                     LDISPIFAFIALNLVDMLVIRNLAAMSRMPQLLSPFL"
     gene            4777..5082
                     /locus_tag="VIO83_01760"
     CDS             4777..5082
                     /locus_tag="VIO83_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEY8330045.1"
                     /translation="MSWFRWDGEDLVLACHLQPKASKDEFAGLHGERLKIRLTAPPVE
                     GKANAHLLAFLGKAFGVAKSQVSLESGELNRQKRVRIHAPQKLPAELAAELQAGRQA"
     gene            5274..6413
                     /locus_tag="VIO83_01765"
     CDS             5274..6413
                     /locus_tag="VIO83_01765"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY8330046.1"
                     /translation="MPTVFAEDSVGLVSPQVLHFKAPLPLACGRQLADYELVYETYGT
                     LNAEHSNAVLICHALSGHHHAAGYHSPDDRKPGWWDSCIGPGKPIDTRKFFVVSLNNL
                     GGCNGSTGPGSRNPDTGRAYGADFPVLTVEDWVHSQARLADALGIEQWAAVVGGSLGG
                     MQALQWSISYPERVRHCLVIASAPKLSAQNIAFNEVARQAILTDPDFHGGHFQEQGTI
                     PKRGLMLARMVGHITYLSDDAMGEKFGRELKTDQLNYDFHSVEFQVESYLRYQGEEFS
                     GRFDANTYLLMTKALDYFDPAAAHGDDLVKTLEPAQANFCVISFSTDWRFSPARSREI
                     VDALVAAKKNVSYLEIESAHGHDAFLIPSPRYIQGFGRYMNRIAV"
     gene            6429..7019
                     /gene="metW"
                     /locus_tag="VIO83_01770"
     CDS             6429..7019
                     /gene="metW"
                     /locus_tag="VIO83_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEY8330047.1"
                     /translation="MRADLEIIQEWIPAGSRVLDLGCGNGELLAWLREHKQVHGYGLE
                     RDPDKIAQCLDRGVNVIEQDLDLGLDHFGDDSFDVVVMTQTLQAVHYPDRILRDMLRV
                     GKTCIITFPNFAHWRCRWYLARKGEMPRSDFLPYTWYNTPNIHFCTFRDFEALCREQR
                     LRVLDRLAVDRDHRLHALTRLWPNLLGEIGIYRVTR"
     gene            7037..7468
                     /locus_tag="VIO83_01775"
     CDS             7037..7468
                     /locus_tag="VIO83_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008092975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HEY8330048.1"
                     /translation="MRRLAILLLTLGLAFPAFAERLQRFGDLEVHYNVFNSSFLQPDV
                     ATASKLTRSKTLGVVTVAVMRAGKAVPAASVSGTVKNLLGQSKALNFQRVTEGEGDTQ
                     AVYHLAQFTMTDREVLTFDLKVQEGGSSQSLTFNQEVFPDE"
     gene            7465..>7719
                     /locus_tag="VIO83_01780"
     CDS             7465..>7719
                     /locus_tag="VIO83_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEY8330049.1"
                     /translation="MKALTELVLASHNAGKLKELQAMLGDAVRVRSVAEFSSHEPEET
                     GLSFVENAILKARHAARVSGLPALADDSGLAVDALGGAPGI"
BASE COUNT         1265 a         2701 c         2486 g         1267 t
ORIGIN      
        1 aagcgcaggt cggcctggcg atccatgccg gggatcagga acaggtcgat cagccagccg
       61 atgccgagca ggccgagggt gcagaaccac agggtgccgg tcaccgggcg cccgtagtag
      121 aagcggtgcg caccgagaaa gccgaacagc cacaacaggt agccgatggt cttgctgtgg
      181 gtgtcccggt tcagtgtcgt catgctgcct cctggcaagg gtcagagggt gacagccagg
      241 gccatcacct ggttcgaccg gcagatcggg cctgcgttcc ccctcagcgg cggcgcaggc
      301 ccggctggtc ctcttcgctg cgctcgaggc tcaggctctg ggtcaggttg ccgaggtgct
      361 cggcgtcggt ttccgagccc agcttgatca tcaggcgcag gtcgttggcc gagtcggcgt
      421 gggccagggc atcctcgtag gtgatctcgc cattgctgta tagctggtac agcgcctggt
      481 cgaaagtctg catgccttgc tcggcggagc gcttcatcag cggcttgagc tcgtgcacct
      541 cgcccttgcg aatcaggtcg gccatcagcg gggtgttgat cagcacctcg atcaccgcgc
      601 ggcggccctt gccgtccggg gtcggcacca gctgctgggc gacgatggca cgcaggttga
      661 gcgacaggtc catccacacc tgctcgtgac gatccgccgg gaagaagtgg atgatgcggt
      721 ccagcgcctg gttggcgttg ttggcgtgca gggtggccag gcacaggtgg ccggtctcgg
      781 cgaaggccac ggcgtagtcc atggtctcgc gggtgcgcac ctcgccgatc atgatcacgt
      841 ccggcgcctg gcgcagggtg ttcttcagcg ccacgtcgta ggactcggta tcgatgccca
      901 cctcgcgctg ggtgacgatg caaccctggt gctggtggat gaactcgatg gggtcctcga
      961 tggaaatgat gtggccctgg gagttcttgt tgcggtagcc gatcatcgcc gccagcgagg
     1021 tcgacttgcc cgcgccggtg gcgccgacga acagcaccag gccgcgcttg gtcatcgaca
     1081 gctgcttgag cacatcgggc aggttgaggt cttcgaaggt ggggatgctg gtctcgatgc
     1141 ggcgcagcac catgccgatc aggttgcgct ggtagaaggc gctgacgcgg aagcgcccga
     1201 tgccacgggc gctaatggcg aagttgcact cgtgggtctc ggccagctcg cggcgctgct
     1261 gctcgttcat caccgacagc acggtctcgc gggtctgctc cggcgccatc gggctcttgg
     1321 tcaccggcag gatcttgccg ttgatcttca tcgacggcgg cacgcccgcg gtgatgaaca
     1381 ggtcggagcc ccccttctcg accatcaggc gcagcagttt ctcgaattcc atggtgttcc
     1441 ctcgatgctt tccgctgccc aggcagcgtg acgggccggt cccccgacgg ggcgaccggc
     1501 cgaacgatta caggctttcc ggcgccttgg ccttttcctt ggccgcgtcg cgggtgacca
     1561 gccccttgct gaccagagtc ttcaggcagg catcgagggt ctgcatgccc agcgcgccac
     1621 cggtctggat cgacgaatac atctgcgcca ccttgtcctc gcggatcagg ttacggatcg
     1681 ccggagtgcc gatcatgatc tcgtgggccg ccacccggcc gccgccgacc ttcttcagca
     1741 gggtctgcga gatcaccgct tgcagcgatt cggaaagcat cgagcggacc atcgacttct
     1801 cctccgccgg gaacacgtcg acgatccggt cgatggtctt ggccgccgag gtggtgtgca
     1861 gggtgccgaa caccaggtga ccggtttccg cggcggtcag cgccaggcgg atggtctcca
     1921 ggtcgcgcat ctcgccgacc aggatgatgt ccgggtcctc acgcagggcc gagcgcagcg
     1981 cctcggagaa gccgagggtg tcgcggtgca cctcgcgctg gttgaccagg cacttcttcg
     2041 actcgtggac gaattcgatc gggtcctcga tggtcaggat gtggtggtac ttggtgttgt
     2101 tcaggtagtc gagcatcgcc gccagggtag tcgacttgcc cgagccggtc gggccggtga
     2161 ccagcaccag accgcgcggc acgtcggtga ttttcttgaa cacctcgccc atgccgaggt
     2221 cttccatggt cagcacgcgc gagggaatgg tccggaacac ggcgccggca ccgcggttct
     2281 ggttgaacgc gttgacccgg aagcgcgcga cgccaggcac ctcgaaggag aagtcggtct
     2341 cgaggaactc ctcgaagtcc ttgcgctgct tgtcgttcat gatgtcgtag atcagcgcat
     2401 gcacctgctt gtgctccatc ggcggcaggt tgatgcggcg cacgtcgcca tcgacgcgga
     2461 tcatcggcgg cagtccggca gagaggtgca ggtccgatgc gccctgcttg gcgctgaagg
     2521 ccagcagctc ggtaatatcc atgggggtcc ccaatcacaa acaagcgggt agaatgccgc
     2581 gacaggcagc ggccgggcaa aaagcggaac ccaatgtcca cgatccacga caatattgca
     2641 aaggtccgca cacgcatccg tgaggcggcg caagcatccg gccgcgatcc gcacagcgtg
     2701 cagctgctgg cggtgagcaa gaccaagccg gcagccgccc tgcgtgcggc gttcgcctgc
     2761 ggccagcgcg acttcggcga gaactatttg caggaagcgc tggccaagca ggccgagctg
     2821 gccgacctgg acctcgcctg gcacttcatc gggccgatcc agtcgaacaa gaccaagccg
     2881 ctggccgagc acttcgcctg ggtgcactcg gtggatcgcc tgaagatcgc cgaacgcctg
     2941 tccgcccagc gttcgccgca cttgccgcca ctgaacatct gcctgcaggt caacgtcagc
     3001 ggcgagacca gcaagtccgg ctgcaacccc gcggagctgc cggcgctggc cgccgcggtg
     3061 gccgcgctgc ccaacctgcg cctgcgcggg ctgatggcca tccccgagcc gaccgccgac
     3121 gtggccgccc agcgcgccgc cttcgcccgc ctgcgcgagc tgaaggacag cctgccgctg
     3181 cccctcgaca ccctgtcgat gggcatgagc gacgacctgg aggccgccat cgccgagggc
     3241 gcgacctggg tacgcatcgg cagcgcgctg ttcggcgccc gcgactaccc tcagacatca
     3301 tccgcgcgtg acgcccgctg aatcggcgcc ccgcccaccg acaaggaatc gcctcatgaa
     3361 tcatccccgc attgctttca tcggcgccgg caacatggcc accagcctga tcggcggcct
     3421 gatcgcccgc ggcgtgcccg ccgaacgcct caccgccagc gagccgcagg ccgagcagcg
     3481 ccagcgcatc gcccgcgaac acggcatcgc ggtgcaggag gacaacgccg ccgccgtggc
     3541 cggcgccgag atcgtggtgc tggcggtcaa gccgcaggtg atgaaggacg tctgcctggc
     3601 cctggcgccg gcgctgccgg ccggcgtgct ggtggtgtcc atcgctgccg gcatcccctg
     3661 cgccagcctc gagcgctggc tgggcgagca ggccatcgtg cgctgcatgc ccaacacccc
     3721 agcgctgctg cacgaaggcg ccagcggcct gtacgccaac gcgcgcacca gcgcgcagca
     3781 gcgcgagcag gccgaggccc tgctggatgc ggtcggcctg gtgctgtggc tggaggaaga
     3841 gcagctgatc gacgcggtga ccgcggtctc cggcagcggc ccggcctact tcttcctgct
     3901 catggaggcg atgaccgccg ccggcgaacg cctcggcctg cccgccgaaa ccgccagcct
     3961 gctggcccgc cagaccgcct tcggcgccgc gcgcatggcg ctggtcagcg acttcgaccc
     4021 ggccgaactg cgccgtcggg tgacctcgcc caacggcacc accgaggcgg cgatcaaggc
     4081 cttccaggcc ggcggtttcg aggctctggt cgaccaggcc ctgcacgccg ccgcccaccg
     4141 ctcggccgag atggccgagc agctcggcca ataaggagcc ccgatgaacg gactcaacac
     4201 tgccgccgtc tacgtgctgc agaccctcgg cagcctgtac ctgctgatcg tcctgctgcg
     4261 tttcatcctg cagctggtgc gcgccgactt ctacaacccg ctcagccagt tcgcggtcaa
     4321 ggccaccaag ccgctgctca acccgctgcg caaggtgatt cccggcttcg gcggcctcga
     4381 cctggcctcc ctggtgctgg cgctgctggt gcagctgctg ctgatgattt tcaccctgct
     4441 gctaatgggc gccttcgacg tcgccctgct gccgcagctg ctgatctggg cggtgatcgg
     4501 cgtcaccgcg ctgttcgtga agatcttctt cttcgccctg atcgccagcg tgatcctctc
     4561 ctgggtcgcc ccgcacagcc acaatcccgg cgcccagctg gtcaaccagc tctgcgagcc
     4621 gctgctgatg ccgatccggc gcatcctgcc caatctcggc gggctggaca tctcgccgat
     4681 cttcgccttc atcgcgctga acctggtgga catgctggtg atcaggaatc tcgccgccat
     4741 gagccgcatg ccgcaactgc tcagcccgtt cctctgatga gctggttccg ctgggacggc
     4801 gaggacctgg tcctcgcctg ccacctgcag cccaaggcga gcaaggacga gttcgccgga
     4861 ctgcacggcg agcgcctgaa gatccgcctc accgcgccgc cggtggaagg caaggccaac
     4921 gcccacctgc tggccttcct cggcaaggcc ttcggcgtgg ccaagagcca ggtcagcctg
     4981 gaaagcggcg aactcaaccg gcagaagcgc gtgcgcatcc atgcgccgca gaagctgccg
     5041 gcggagctgg ccgccgagct gcaggccggg cgccaagcgt aggggggggt tagccgcaaa
     5101 gcggcgtaac ccaccaagga tgccgccaga catcctcccg ctgccggcct gacggccggt
     5161 tggcgggtta cggcctgcgg cctaacccag cctacgccca ccacaggcgc gcccttgccc
     5221 ctgtgggcag cgctctttag actggcgcac ttcatttttc gagagcaggg tcgatgccca
     5281 ccgtcttcgc cgaagattcc gtcggcctgg tcagccccca ggtactgcac ttcaaggccc
     5341 ccttgccgct cgcctgcggg cgccaactgg ccgactacga gctggtctac gaaacctacg
     5401 gcaccctcaa tgccgagcac agcaacgccg tgctgatctg ccatgccctc tccggccacc
     5461 atcacgctgc cggctaccac agccccgacg accgcaagcc gggctggtgg gacagctgca
     5521 tcggccctgg caagccgatc gacacccgca agttcttcgt cgtcagcctc aacaacctcg
     5581 gcggctgcaa cggctccacc ggccccggca gccgcaaccc ggacaccggc cgcgcctacg
     5641 gcgccgactt cccggtgctg accgtggagg actgggtgca cagccaggcc cgcctggccg
     5701 acgccctcgg catcgaacag tgggccgcgg tggtcggcgg cagcctcggc ggcatgcagg
     5761 ccctgcagtg gagcatcagc tatcccgagc gcgtgcggca ctgcctggtg atcgcctcgg
     5821 cgcccaagct gtcggcgcag aatatcgcct tcaacgaagt ggcgcgccag gccatcctca
     5881 ccgacccgga cttccacggc ggccacttcc aggagcaggg caccatcccc aagcgcggcc
     5941 tgatgctggc gcgcatggtc ggccacatca cctacctgtc cgacgacgcc atgggcgaga
     6001 aattcggccg cgagctgaag accgaccagc tcaactacga cttccacagc gtcgagttcc
     6061 aggtcgaaag ctacctgcgc taccagggcg aggagttctc cgggcgcttc gacgccaaca
     6121 cctacctgct gatgaccaag gcgctggact acttcgaccc ggccgccgcc cacggcgacg
     6181 atctggtgaa gaccttggag ccggcgcagg cgaacttctg cgtgatctcc ttctccaccg
     6241 actggcgctt ctcgccggcg cgctcgcggg agatcgtcga tgcgctggtg gcggcgaaga
     6301 agaacgtcag ctacctggag atcgagtcgg cccacggcca cgacgccttc ctcatcccga
     6361 gcccgcgcta catccagggt ttcggtcgct acatgaaccg tattgccgtg taaggacagc
     6421 tgccgaacat gcgtgccgat ctggaaatca ttcaggaatg gatccccgcc ggcagccggg
     6481 tcctcgacct cggctgcggc aatggcgagc tgctggcctg gctgcgcgag cacaagcagg
     6541 tgcacggcta cggcctggag cgcgacccgg acaagatcgc ccagtgcctg gaccgcgggg
     6601 tcaacgtcat cgagcaggat ctcgacctcg gtctcgacca cttcggcgac gacagcttcg
     6661 acgtcgtggt gatgacccag accctgcagg cggtgcacta tcccgaccgc atcctcaggg
     6721 acatgctgcg ggtcggcaag acctgcatca tcaccttccc caacttcgcc cactggcgct
     6781 gccgctggta cctggcgcgc aagggcgaga tgccgcgttc ggacttcctg ccctacacct
     6841 ggtacaacac gccgaacatc cacttctgca ccttccgcga cttcgaggcg ctgtgccgcg
     6901 agcagcgcct gcgggtcctc gatcgcctgg cggtggaccg cgaccaccgg ctgcatgcac
     6961 tcacgcgcct gtggcctaat ctgttgggtg agatcggtat ctaccgagtc acccgctgac
     7021 cccaaggaga actcccatgc gtcgcctcgc catcctcctg ctcaccctgg gtctggcctt
     7081 cccggccttt gccgaacgcc tgcagcgctt cggcgacctc gaggtgcact acaacgtgtt
     7141 caactccagc ttcctgcagc cggatgtggc caccgcgtcc aaactgaccc gcagcaagac
     7201 cctgggcgtg gtcactgtcg cggtcatgcg tgccggcaag gccgtgcccg cggccagcgt
     7261 ctccggcacg gtgaagaacc tgctcggcca gagcaaggcg ctcaacttcc agcgcgtcac
     7321 tgaaggtgaa ggcgacacgc aggcggtcta ccacctggcg cagttcacca tgaccgaccg
     7381 cgaggtgctg accttcgacc tcaaggtgca ggaaggcggc agcagccaaa gcctcacctt
     7441 caaccaggaa gtattccccg acgaatgaaa gccctcaccg aactggtact ggccagccac
     7501 aacgccggca aactcaagga actgcaggcc atgctgggcg acgccgtgcg cgtgcgctcg
     7561 gtggccgagt tcagcagcca tgagccggaa gagaccggcc tgagcttcgt cgagaacgcc
     7621 atcctcaagg cccgtcacgc cgcgcgggtg tccggcctgc cggcgctggc cgacgactcg
     7681 ggcctggcgg tggacgccct cggcggcgcc ccgggcatc
//
LOCUS       DATCUN010000053         7278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_20876, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000053 DATCUN010000000
VERSION     DATCUN010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20876"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..709
                     /locus_tag="VIO83_01785"
     CDS             125..709
                     /locus_tag="VIO83_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY8330050.1"
                     /translation="MQALDALINRVSVARLGEPAPSAQQRELLFRAALRAPDHGQLRP
                     WRFLTVEGEAREALGELFARAQCVRDPQAPAALLDKARAMPLRAPLLIVVIASPKAHP
                     KVPESEQLLAAGCAAHGVLLAAHAQGIGAIWRTGELTGDATVKAGLGLAAHEQIIGFL
                     YLGTPLGELRTPQALEPADFVAAWSSDLGAVGQG"
     gene            complement(680..2011)
                     /locus_tag="VIO83_01790"
     CDS             complement(680..2011)
                     /locus_tag="VIO83_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020477370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8330051.1"
                     /translation="MRSLFWRIFATFWLAIALVAGLSLLLGQALNQDRWILAHHPGVR
                     DVAAEWTARFEQQGPMAAQDFLVQHKHRQHADVQVLDETGDQLVRGTFPPRAAAFEAR
                     QHNQKRLPWRRLTEEYRSPASGQIYLFIYRIPHQELAAWHRDSLLWPLSALLIAVVVL
                     SLLSVLLTLSITRPLDRLRGAVHDLGQAAYQQNSLARLSTRRDELGTLARDFNRMGAR
                     LQDLIASQRQLLRDVSHELRSPLARLRVALALAERGGDEERARLWPRLVQECDRLEAL
                     ISEILLLARVDAESSKAQPVDLPALLGKLREDARLLAPQQEVRLDIEAGLRLQGWPEL
                     LEQALDNLLRNALRFNPAGQPLEISAHRDGQRLLLEVRDHGPGAASEHLAKLGEPFFR
                     APGQDSPGHGLGLAIARRAAQRHGGQLVLANHPGGGFVARLELPLTDSAEV"
     gene            complement(2083..2547)
                     /locus_tag="VIO83_01795"
     CDS             complement(2083..2547)
                     /locus_tag="VIO83_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTXXQ domain protein"
                     /protein_id="HEY8330052.1"
                     /translation="MRKTLTALLLAASLPTLAIAAPGEDDMGPYHMRGEGYHDGHMMR
                     GDGHHGGSMMGGMDLTPEQRQKVRSAMREQMQTHRDITQRYLDKLPAADKAAMQKEQE
                     ANRDKHQKAIRDTLSAEQQKQFDQMQKDMEKRRAERREFEAWKAERDKKAAQ"
     gene            complement(2652..3338)
                     /locus_tag="VIO83_01800"
     CDS             complement(2652..3338)
                     /locus_tag="VIO83_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010450018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8330053.1"
                     /translation="MNDLLLIDDDRELTDLLAAWLGQEGFRVRACHDGVSARQALAEQ
                     APDAVVLDVMLPDGSGLELLKQLRSEHSELPVLMLSARGEPLDRILGLELGADDYLAK
                     PCDPRELTARLRAVLRRVQPGGGSASNTRLELGDLSYSPNRGVAMLGEQEITLTVSES
                     HILEALLKQPGEPLGKQALAQLALGRKLTLYDRSLDMHVSNLRKKLGLHADGRPRILA
                     SRGRGYYYAP"
     gene            complement(3381..3680)
                     /locus_tag="VIO83_01805"
     CDS             complement(3381..3680)
                     /locus_tag="VIO83_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY8330054.1"
                     /translation="MLYAIIASDAANALEARLANRPAHLERLNALKDAGRLVLAGPHP
                     AIDSNDPGPAGFTGSLVVAEFDSLEAARAWADADPFCAAGVYAEVVVKPFRQTLP"
     gene            complement(3682..4278)
                     /locus_tag="VIO83_01810"
     CDS             complement(3682..4278)
                     /locus_tag="VIO83_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEY8330055.1"
                     /translation="MKQFIDFIPLILFFITYKMEPRTLELAGHSLSVGGVYSATAVLI
                     GASLVVYGLIFATQRKLDKGQWITLLACLVFGGMTLTFHSETFLKWKAPAVNWLFALG
                     FAASQFIGDKPLIQRIMGHAVALPQPLWTRLNLAWVAFFIFCGLANLYVAFTFHEYWV
                     DFKVFGSLGLTLLFMLGQGVFLARHMQDAASEKIAPKD"
     gene            4345..5205
                     /locus_tag="VIO83_01815"
     CDS             4345..5205
                     /locus_tag="VIO83_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003314714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY8330056.1"
                     /translation="MYVDLHCHSTASDGMLTPAGVVARAHERGVGVLALTDHDTLDGL
                     PEARVEAERRGIELIDGIELSCTWNGATIHVLGYAFAPEAPVLQRAIAALHEGRWARS
                     EEIDRRLEAKGMPGCLEGARMVQQERGDSGNAPSRPHFAEFMVRAGFVKDHAQAFQRW
                     LGAGKLGDVKQHWPTLESTLETLRDSGASISLAHPCHYDFTRTKRRKLVAAFVEGGGH
                     ALEVSNGPQVDSQVGMLANLAREFGLLATAGSDFHGPRQWSELGSYRGLPADVTLLWP
                     RLARMEHPTS"
     gene            5215..5844
                     /locus_tag="VIO83_01820"
     CDS             5215..5844
                     /locus_tag="VIO83_01820"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY8330057.1"
                     /translation="MSQFFQIHAENPQARLVKQAVDIIREGGVAVYPTDSSYALGCQI
                     GNKSAIDRIRHLRRLDDKHNFTLLCRDLSQLGEYAKVDTSAFRLLKAHTPGPYTFILD
                     ATREVPRMLLHPKRRTIGLRVPGNPITHALLAELGEPLMSVSLIMPGDEQPLSDPYEI
                     RDRLDKLVDLIIDGGYGSLEASTVISLLGGEPEVIREGCGDPEPFREPA"
     gene            5981..6745
                     /locus_tag="VIO83_01825"
     CDS             5981..6745
                     /locus_tag="VIO83_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEY8330058.1"
                     /translation="MYIPPDALEVFLEAFEGPLDLLLYLIRKQNIDILDIPVAEITRQ
                     YMGYVELMKSVHLELAAEYLVMAAMLAEIKSRMLLPRSSEAVDDEEDPRAELIRRLQE
                     YERFKQAAEELDALPRLGRDLHTVLLPAPEARARTLLPEVSLDEVLLAMAEVMRRAEL
                     FESHQVTRELLSTRDRMSDVLARLQGGGFVAFIALFTVEEGKLGVVVTFMAILELVKE
                     SLVELVQNEPFTPIHVRLRGESEAVEQSLVVESVAD"
     gene            complement(6789..>7278)
                     /locus_tag="VIO83_01830"
     CDS             complement(6789..>7278)
                     /locus_tag="VIO83_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HEY8330059.1"
                     /translation="LSRKQRGSKNRAKARQKVARLHAKIADTRLDGLHKLSRRLINEN
                     QVVCVESLAVKNLIRNRCLSKSIADAGWGELVRQLEYKAAWAGRQLIKIDRWIPSSKR
                     CSCCGHTLASLPLKVRDWTCPACTTEHDRDLNAAINIKAAGLAVLALGENVSGIGRVP
                     VS"
BASE COUNT         1109 a         2324 c         2620 g         1225 t
ORIGIN      
        1 gccgccgctg accccgccgc tggagccgga tatggccccg gaccgcgtgg cctgaggtcc
       61 ggccgggcga cggcgaaaca ttcggcgcta gaatgcgctc tttgccatcc gacaggagaa
      121 ttccatgcag gctctcgacg ccctgatcaa tcgtgtttcg gtggcgcgcc tgggggaacc
      181 ggcgccttcg gcgcagcagc gcgagctgct gttccgggcc gccttgcgcg cccctgatca
      241 tggtcagcta cggccctggc gctttctcac cgtggaaggc gaggcccgcg aggcgctcgg
      301 cgagctgttt gcccgcgccc agtgcgtgcg cgacccgcag gcgccggcgg cactgctgga
      361 caaggcccgc gccatgccgc tgcgtgcgcc actgctgatc gtggtgatcg ccagcccgaa
      421 ggcgcacccc aaagtgccgg agtcggagca gttgctggcg gccggctgtg cggcgcatgg
      481 tgtgctgctg gccgcgcatg cccagggcat cggcgccatc tggcgcaccg gcgagctgac
      541 cggcgatgcc acggttaagg ccgggctggg gctggccgcg cacgagcaga tcatcggctt
      601 cctttatctg ggcacgccgc tgggcgaact gcgcacgccg caagcgctgg agccggcgga
      661 cttcgttgcc gcctggtcgt cagacctcgg cgctgtcggt caggggtagt tcgaggcggg
      721 caacaaagcc gccgccggga tgattggcca gcaccagctg gccgccatgg cgctgcgccg
      781 cgcggcgggc gatggccaga cccaggccat gacccggact gtcctggccg ggggcgcgga
      841 agaacggttc acccagcttg gccaggtgtt cgctggccgc gccgggacca tgatcgcgca
      901 cttccagcag caggcgctgt ccgtcgcggt gggcgctgat ttccagtggc tggcccgcag
      961 ggttgaagcg cagggcgtta cgcagcaggt tgtccagcgc ctgctcgagc agctccggcc
     1021 agccttgcag gcgcagtccc gcctcgatgt cgaggcggac ttcctgctgc ggtgccagca
     1081 gccgcgcatc ctcgcgcagc ttgccgagca gtgccggcag gtccaccggc tgcgccttgc
     1141 tgctctcggc atccacccgc gccagcagca gaatctcgct gatcagtgct tccagtcgat
     1201 cgcattcctg caccaggcgc ggccacagcc gtgcgcgttc ctcgtcgccg ccgcgctcgg
     1261 ccagcgccag ggctacgcgc aggcgcgcca gcggcgagcg cagttcgtgg gacacgtcgc
     1321 gcagcagctg gcgctggctg gcgatcaggt cctgcaggcg ggcgcccatg cggttgaagt
     1381 cgcgcgccag cgtgcccagc tcgtcgcgtc gggtggacag gcgagccagg ctgttctgct
     1441 ggtaggcggc ctggccgagg tcgtgcaccg caccgcgcag gcggtcgagc gggcgggtga
     1501 tggacagggt cagcagcacg ctgagcaggc tcagcaccac cacggcgatc aacagggcgc
     1561 tgagcggcca cagcaggctg tcgcggtgcc aggccgccag ctcctggtgc gggatgcggt
     1621 agatgaacag gtagatctgg ccgctggccg ggctgcggta ctcctcggtc agccgtcgcc
     1681 agggcaggcg tttctggttg tgctggcgcg cctcgaaggc cgcggcgcgg ggcgggaagg
     1741 tgccgcggac caactggtcg ccggtttcgt cgagcacctg cacgtcggcg tgttggcgat
     1801 gcttgtgctg caccaggaaa tcctgggcgg ccatcggacc ttgctgctcg aagcgcgcgg
     1861 tccattcggc ggcgacgtcg cgtacgccgg gatgatgggc gaggatccag cgatcctggt
     1921 tgagggcctg gccgagcagc agcgacaggc cggccaccag ggcgatggcc agccagaagg
     1981 tggcgaagat gcgccagaac agtgagcgca tgggcgcagc tccgtcgttg cacgtacggg
     2041 gataaaaaaa ccgggacgtc ccgctggacg ccccggccgg ggttactgag cggctttctt
     2101 gtcgcgctcg gccttccagg cttcgaactc gcggcgctcg gcgcggcgct tttccatgtc
     2161 cttctgcatc tggtcgaact gcttctgctg ctctgcgctc agggtgtcgc ggatggcttt
     2221 ctggtgcttg tcgcggttgg cttcctgctc cttctgcatg gcggccttgt cggcagccgg
     2281 cagcttgtcc aggtagcgct gggtgatgtc gcggtgggtc tgcatctgct cgcgcatggc
     2341 gctgcgcact ttctggcgct gctcgggcgt caggtccatc ccccccatca tggagccgcc
     2401 gtggtggccg tcgccgcgca tcatgtggcc gtcgtggtag ccctcgccgc gcatgtggta
     2461 cgggcccatg tcgtcctcgc cgggcgcagc gatagccagg gtcggcaggc tggcggcgag
     2521 gagcagggcg gtcagggtct tgcgcatgat ctgtttcctt ctcgatgacg gcgtggtcgc
     2581 cggatgagtt cagtctaggg cgcgcaaggt caaccgtggt cagggcaggg taaagactgg
     2641 gtaaaggccg ctcagggggc gtaatagtag ccgcgcccac ggctggcgag gatgcgcggg
     2701 cggccgtcgg cgtgcaggcc gagcttcttg cgcaggttgc tgacgtgcat gtcgaggctg
     2761 cggtcgtaga gggtcagctt gcggccgagg gcgagctggg cgagggcctg cttgcccagc
     2821 ggctcgcccg gctgcttgag cagggcctcg aggatgtggc tttccgagac ggtcagggtg
     2881 atttcctgtt cgccaagcat ggccacgccg cggttcgggc tgtagctcag gtcgcccagc
     2941 tccaggcggg tgttgctggc gctgccgccg cccggctgta cccggcgcag cacggcccgc
     3001 agacgggcgg tcagttcgcg cgggtcgcag ggcttggcca ggtagtcgtc ggcaccgagc
     3061 tccaggccga ggatgcggtc cagcggttcg ccgcgcgccg aaagcatcag caccggcagt
     3121 tccgagtgct cgctgcgcag ctgcttgagc agctccaggc cgctgccgtc ggggagcatg
     3181 acatccagca cgactgcgtc cggcgcctgt tcggccagcg cctggcgggc acttaccccg
     3241 tcgtggcagg cccggacacg aaagccttcc tggccaagcc aggcagccag caggtcggtg
     3301 agctcgcggt cgtcgtcgat cagcagcagg tcgttcatgg ggcgctcggc tggcgcgccg
     3361 gacaagggcg cgccggtggc tcagggcagg gtctggcgga acggcttgac taccacctcg
     3421 gcgtagacgc cggcggcaca gaaggggtcg gcgtcggccc aggcgcgggc agcttcgagg
     3481 gagtcgaatt cggcgacgac caggctgccg gtgaagccgg ccggacccgg atcgttgctg
     3541 tcgatggccg gatgcgggcc ggcgagtacc aggcggccgg cgtctttcag cgcgttgagg
     3601 cgttcgaggt gggccgggcg gttggccagg cgggcttcga gggcgttggc agcgtcgctg
     3661 gcgatgatgg cgtagagcat gtcagtcctt gggggcgatt ttttcggagg cggcgtcttg
     3721 catgtggcgg gcgaggaaca cgccctggcc gagcatgaac agcagggtca ggccgaggct
     3781 gccgaagact ttgaagtcga cccagtattc gtggaaggtg aaggccacgt agaggttggc
     3841 caggccgcag aagatgaaga acgccaccca cgccaggttc aggcgggtcc acagcggctg
     3901 gggcagggcc acggcgtggc ccatgatgcg ctggatcagc ggtttgtcgc cgatgaactg
     3961 gctggcggcg aagccgaggg cgaacagcca gttgacggcg ggcgccttcc acttgaggaa
     4021 agtctcgctg tggaaggtca gggtcatgcc gccgaacacc aggcaggcga gcagggtgat
     4081 ccactggccc ttgtccagct tgcgctgggt ggcgaagatc aggccgtaga ccaccaggga
     4141 ggcgccgatc agcacggcgg tggcgctgta cacgccgccg acgctcaggc tgtgcccggc
     4201 gagctcgagg gtgcgcggct ccatcttgta ggtgatgaag aacaggatca gcgggatgaa
     4261 gtcgatgaat tgtttcacgg cacacgccac aggcaggatg agggggcata ataacagacc
     4321 aacaaagacg gaaaagcctc gcccatgtac gttgatctgc actgccacag taccgcctcc
     4381 gacggcatgc tcacgcctgc cggagtggtg gcgcgggcgc atgagcgcgg cgtgggcgtg
     4441 ctggctctga ctgaccacga taccctcgat ggcctgcccg aggcgcgtgt cgaggccgag
     4501 cgtcggggta tcgagctgat cgacggcatc gagctgtcct gcacctggaa cggcgcgacc
     4561 atccatgtgc tcggctatgc cttcgcgccc gaggcgccag ttctgcagcg ggccatcgcg
     4621 gcactgcacg aggggcgctg ggcacgctcc gaggagatcg accggcgcct ggaggccaag
     4681 ggcatgcccg gctgcctgga aggcgcccgg atggtgcagc aggagcgcgg cgacagcggc
     4741 aatgcgccgt cgcggccgca cttcgccgag ttcatggtgc gcgccggctt cgtcaaggat
     4801 catgcccagg ccttccagcg ctggctgggg gccggcaagc tgggcgacgt caagcagcat
     4861 tggccgacgc tggagagcac gctggaaacc ctgcgcgact ccggcgcctc gatcagtctg
     4921 gcgcatccct gtcattacga ctttactcgg actaaaagac gcaagttggt ggcggcattc
     4981 gtcgaggggg gcgggcatgc gctggaagtg tccaacggcc cgcaagtgga cagtcaggtc
     5041 ggcatgctgg ccaacctcgc ccgcgagttc ggcctgctgg ccactgccgg cagtgatttt
     5101 cacgggccgc ggcagtggtc cgaactgggc agctaccgtg gtttgcccgc cgatgtgacg
     5161 ctgttgtggc cgcgtttggc ccggatggaa caccctacca gttaaggagc ggccatgagc
     5221 cagttcttcc agattcatgc ggaaaacccg caggcacggc tggtcaagca ggccgtggac
     5281 atcattcgcg agggcggcgt ggctgtctat ccgaccgatt cctcctatgc cctcggctgc
     5341 cagatcggca acaagagcgc gatcgaccgc atccgccacc tgcgccggct ggatgacaag
     5401 cacaacttca ccctgctgtg ccgcgacctg tcgcagctcg gcgagtacgc caaggtcgac
     5461 accagcgcct ttcgcctgct caaggcgcac acgccggggc cctacacctt catcctcgac
     5521 gccacccgcg aagtgccgcg catgctgctg catcccaagc gccgcaccat cggcctgcgc
     5581 gtgcccggca accccatcac ccacgcgctg ctggccgagc tgggcgagcc gctgatgagc
     5641 gtgagcctga tcatgccggg tgacgagcag ccgctgagcg atccctacga gatccgcgac
     5701 cgcctggaca agctggtcga tctgatcatc gatggcggct acggcagcct cgaagcctcc
     5761 acggtgatca gtctgctcgg cggcgagcct gaggtgatcc gcgaaggctg cggcgatccc
     5821 gagccgttcc gggaaccggc ctgatgcgcg ctgattgttg caggctggtc gggtgagcga
     5881 gcaggcattg cccgacgcgg cggtggcggc cgaggccatc cccgagcagc tgccactggc
     5941 gctggtctat ggcgcggcct tcaccgagct gccgcaggac ctgtacatcc cgccggatgc
     6001 cctcgaggtg ttcctcgagg ccttcgaggg gccgctggac ctgttgctgt acctgatccg
     6061 caagcagaac atcgatatcc tcgacatccc ggtggccgaa atcacccgcc agtacatggg
     6121 ctatgtcgag ctgatgaagt cggtgcacct ggagctggcg gccgaatacc tggtgatggc
     6181 ggcgatgctc gccgagatca agtcgcgcat gttgctgcca cgctccagcg aggcggtgga
     6241 cgacgaggaa gatccgcgcg ccgagctgat ccgccggctg caggaatacg agcgcttcaa
     6301 gcaggccgcc gaggagctgg atgccctgcc gcggctgggt cgcgacctgc acaccgtgct
     6361 gctgccggcg ccggaggcac gggcgcgcac gctgctgccg gaggtgagcc tggacgaggt
     6421 cctgctggcg atggccgagg tgatgcgccg cgccgagctg ttcgagagcc accaggtcac
     6481 ccgcgagctg ctgtccaccc gcgatcgcat gagcgatgtg ctggcgcgct tgcagggcgg
     6541 cggcttcgtc gccttcatcg cgctgttcac cgtcgaggag ggcaagctcg gggtggtggt
     6601 gaccttcatg gcgatcctcg agctggtcaa ggagtcgctg gtcgagctgg tacagaacga
     6661 gccgttcacg cccatccatg tgcgcctgcg cggtgaaagt gaagcggttg aacagagtct
     6721 ggttgtggag agcgttgccg attgactgct ctccgccctg aagggcggag attcccaatt
     6781 catcgagatc aggacaccgg tacacgaccg atgccactta cgttctctcc aagggctaac
     6841 accgccagcc cggcggcttt gatgttgatc gcggcgttca ggtcgcggtc gtgttccgtg
     6901 gtgcaggccg gacaggtcca gtcgcggact ttgagtggca gcgaggccag ggtgtgaccg
     6961 cagcacgagc agcgcttgct gctggggatc cagcggtcaa tcttgatcag ctggcgccca
     7021 gcccatgccg ccttgtactc gagctggcgc accagctcac cccagccggc atcggcgatg
     7081 gacttgctca ggcaccggtt gcggatcagg ttcttcaccg ccaacgactc gacgcagacc
     7141 acttggtttt cgttgatcag tctgcgcgac agcttgtgca agccgtcgag tcgggtgtcg
     7201 gcgattttcg cgtgcaggcg agcgaccttc tgccgggcct tggcccggtt cttcgaaccg
     7261 cgctgcttgc ggctcagc
//
LOCUS       DATCUN010000054        11160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_189905, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000054 DATCUN010000000
VERSION     DATCUN010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11160
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189905"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="VIO83_01835"
     CDS             complement(<1..1335)
                     /locus_tag="VIO83_01835"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEY8330060.1"
                     /translation="MYVYDEYDQRIIEERVAQFRDQTRRFLAGELAEEEFRPLRLQNG
                     LYVQRHAPMLRVCVPYGLLSAPQLRMLAKLARDYDKNYAHISTRQNVQYNWPKLEDVP
                     DMLAELASVQMHAIQTSGNCIRNVTTDQFAGVAADEVIDPRPWCEIIRQWATFHPEFA
                     HLPRKFKIAVNGSAADRAAIEVHDIGLEPVRNEAGELGFRVLVGGGLGRTPIVGSFIN
                     EFLPWQHLITYLEAILRVYNRYGRRDNKYKARIKILVKALTPEVFAERVAAEWAHLKD
                     GPSTLTEAELQRVAQHFVDPAYQALEDQGAALAALDAEHPGFARWRSRNVVAHKKPGY
                     AAVTLSLKPTGIAPGDVTDKQFDAIADLAERYSFGEARTSHEQNIILADVEQAQLFAL
                     WNELRELGLATPNIGLLTDIICCPGGDFCSLANAKSIPIAEAIQRRFDDLDYV"
     gene            complement(1547..3145)
                     /locus_tag="VIO83_01840"
     CDS             complement(1547..3145)
                     /locus_tag="VIO83_01840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019751999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY8330061.1"
                     /translation="MRFSPMFRVDLRRLILLLSITAALLTLINSFYVGYQVQRDLLIQ
                     QTLEANRVYAAKLAESTEGLLASARQQLAYSAARLPALLEQPQQLAAETERLKQQTDT
                     FNSAYIVRDDLTLLAISPAMLLPAGSLLNSAGARESLEKRQPLVSAPFVSTAGKLVVM
                     ISQPIIAADGRYLGYVGGSIYLRERSILNSLLGEHFYRDGSYIYVVDQHGQLLYHRDA
                     ERVGEPIHSNPAVAAVIAGQAGSQQIVNSRDIDMLAGYAPVASTGWGIVAQRPTANTL
                     AELDGLMLSILRNALPFSLLSLLGIWWLARLIAQPLWQLASSARQMDSQTAPGEIRQV
                     GAWYFEAAELKRALIAGLAQFDRKIGKLNLDTLTDPLTGLYNRRGLQLTLEQWQARTQ
                     PFAAVALDIDHFKQVNDNHGHAVGDLQLQHLARLMRESSRGGDVLCRVGGEEFLILLP
                     DVDLATATQVAERLRLRMETCTALPCGPVTLSIGVAHWPDSAAAIDRVLEMADQALYT
                     AKRSGRNLVVVAPSERQPSDASLS"
     gene            3315..3437
                     /locus_tag="VIO83_01845"
     CDS             3315..3437
                     /locus_tag="VIO83_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330062.1"
                     /translation="MNQQVKTQVASIADSRADAWAALSLIMIGVTTVILWVSQQ"
     gene            complement(3487..3729)
                     /locus_tag="VIO83_01850"
     CDS             complement(3487..3729)
                     /locus_tag="VIO83_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HEY8330063.1"
                     /translation="MSEQQPQRNDDQDESPLTFRQMLQSVLAAAFGVQSGKNRARDFS
                     RGKPSHFILLGLLFTGVFILLLYSVVRLVLQLSGVK"
     gene            complement(3798..5117)
                     /locus_tag="VIO83_01855"
     CDS             complement(3798..5117)
                     /locus_tag="VIO83_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY8330064.1"
                     /translation="MPQRLRQILILGLPIIGGMLSQSLINLVDAAMVGHLGETALAGV
                     GIGSYANFMAIALVMGLGSGVQALVARRRGEGRLAETGGPLTAGLLLAVLVGLPLGLG
                     GWLAAPWIIGLLSNDPAVAAIGSDYFQWRVLAVAAVGMNFAFRGYWNGSHRSGLYLRL
                     LLGIHLFNAALSYCLVHGLLGLPAMGAQGAGLGTCIAQYAGSLIFASQTWRVAPAAGF
                     RWQRPPPVEIQRLSRLSLPNSLQQLFFATGITLLFWIIAQIGTAELAVAHVLVNLALL
                     LILPAVGLGMAATTLVSHSLGAGDVDAAHRWGWDVVRVAIGGLALLGTPFWLFPEAIL
                     GLFVSAPQLIELGTWPLRLTGLGMVLDAGALVLAQALLGAGASRTVMLVTLGNQWLVV
                     LPLTYLLGPVLGYGLLAIWSLYILQRGLASAIFVLMWQRRAWARIEI"
     gene            5309..6718
                     /locus_tag="VIO83_01860"
     CDS             5309..6718
                     /locus_tag="VIO83_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY8330065.1"
                     /translation="MTHEPQHPGELQRSLKNRHIQLIALGGAIGTGLFLGSAGVLQSA
                     GPSMILGYAIAGWIAFLIMRQLGEMIVEEPVAGSFSHFAHRYWGGFAGFLSGWNYWAL
                     YILVGMAELTAVGKYIQFWWPDVPTWATAAAFFVLINAINLTNVKFFGEAEFWFAIIK
                     VVAVLGMIVLGLWLLLSGTGGEQAQVSNLWAHGGFFPHGVGGLVMALAFIMFSFGGLE
                     LVGITAAEAAEPKKVIPKAINQVVYRILIFYVGALGVLLCLYPWDALLAALNASGDPY
                     SSSPFVQIFSLLGSEVAANILNFVVLTAALSVYNSGVYCNSRMLYGLAEQGNAPRAFL
                     RIDKRGVPVIAIGASALITLLCVLINYTLPKGALELLMSLVVAALVINWAMISLIHLK
                     FRAAKQREGVQLEFKALWYPFGNYLCLAFVLLILGVMWQLEGIRLSVYAIPLWLLFIG
                     LCYRLKGAFGAAPSPRIVR"
     gene            complement(6906..7250)
                     /locus_tag="VIO83_01865"
     CDS             complement(6906..7250)
                     /locus_tag="VIO83_01865"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY8330066.1"
                     /translation="MNPLSQAHCEACRAGAPQVSDAELAELIRQIPDWNIEVRDGVMQ
                     LEKVFSFPDFQRALNFTNAVAAIAEVEGHHPALLTEWGKVTVTWWTHKIGGLHRNDFI
                     MAARTDQLAKTA"
     gene            complement(7330..8646)
                     /locus_tag="VIO83_01870"
     CDS             complement(7330..8646)
                     /locus_tag="VIO83_01870"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat-containing protein kinase
                     family protein"
                     /protein_id="HEY8330067.1"
                     /translation="MHTLEQLRRGELAGIRRLDLSAGLTELPEEIFDLADSLEVLNLS
                     GNQLSQLPAELPRLRKLRILFCSDNPFTTVPEVLGDCARLEMVGFKSCQVQQVPAAAL
                     PPLLRWLILTDNRLQRLPEQLGECRRLQKLALAGNRLDALPASLADCQNLELLRIAAN
                     RLDALPGWLTELPRLAWLAFAGNPFSDSREAQTLAEHPLPPIARSSIALQEVLGQGAS
                     GVIHRAEWHQPAQPPQAMAAKLFKGNVTSDGLAHSEMAACIAAGEHANLIRVAGPLAA
                     QPDTAPGLLLELIAPTFQPLAGPPSLASCTRDCYATERRFGVDEALHIARGVASAVAH
                     LHERGILHGDLYAHNLLVNAAGDCLLGDFGAASFFDPGSASGQALQRIEARAFGYLLE
                     ELLQRCPADEQIGRQQRLRQLQEQCVTEPIEQRPDFASLDACLAAI"
     gene            complement(8654..9307)
                     /gene="grxB"
                     /locus_tag="VIO83_01875"
     CDS             complement(8654..9307)
                     /gene="grxB"
                     /locus_tag="VIO83_01875"
                     /inference="COORDINATES: protein motif:HMM:NF007702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HEY8330068.1"
                     /translation="MKLYLYDHCPFCVRAEMVAHYKQVPVEQVYLLNDDEASCFALIG
                     AKQVPILQFDDGRAMGESLDIARKLDELGNPAQVIRPHGDYLAIQEHMNQVRLANWCL
                     LFPRDIALGLPEFATQSARDYFQAKKEQIIQRPFAQALAETAEHRAVVEQMLASLPPL
                     DLPSAHGNTLGWDDVLLYPGLRNLSMVKGLAFPPAVRAYVEEVARITATQTYFERAV"
     gene            complement(9340..9801)
                     /locus_tag="VIO83_01880"
     CDS             complement(9340..9801)
                     /locus_tag="VIO83_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEY8330069.1"
                     /translation="MLLNMLVGLPTMLLCLLMEAYIVVLCLRYYVGYHTRPSGNGAGN
                     KAILWDLLVLGVVMLLMMLGYFVQITLWAGLFMLLGEFDEFATAIYYSGVTFATLGYG
                     DIVLSKPWRLLGALEAGNGVLMLGISTGVMTAAVKEVIKHNIERLKPGLNG"
     gene            complement(9812..10846)
                     /locus_tag="VIO83_01885"
     CDS             complement(9812..10846)
                     /locus_tag="VIO83_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008015031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2955 domain-containing protein"
                     /protein_id="HEY8330070.1"
                     /translation="MATPREPRAQRALRLASGVALCLTVSYGLGLELSFIAPIFALFL
                     LAKLNQPLSFKAGLGLTLVVMLTTGSGLLLIPLLRYAPVSGVLLVGLCLFLAFRHGLS
                     GGNSLVTTFLVVGLTMISAAGTAEFALALKVISALVQGLLLAVLMLPVSHWLFPEPAG
                     AQAPPAAPALPAQETGWVALRAALVVMPAYLLALIDPASYMAIILKSVGLGQQSCTAT
                     VRDAARELIGSTLLGGLLAMLFWWALGLFVHLWMFFLWMLLFGLLVARKLYGFSPTRQ
                     SPGFWLNSLVTMIILLGLSVQDSANGKDVYSAFVVRMALFVAISLYAWLMVYLLDQYR
                     QRRAIRQPGC"
     gene            complement(10855..>11160)
                     /locus_tag="VIO83_01890"
     CDS             complement(10855..>11160)
                     /locus_tag="VIO83_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY8330071.1"
                     /translation="VLPGEVFEGRVRSVGYGVSVGQSTAPGSLPSVENSRDWLRPAQR
                     FPVIVEFAAGEAARLAKLRGIRVGGQADVMAFPTEGNPLNPLGRLFVHLMSWLSYAY"
BASE COUNT         1873 a         3506 c         3943 g         1838 t
ORIGIN      
        1 cacgtagtcg aggtcgtcga agcggcgctg gatggcttcg gcgatcggga tcgacttggc
       61 gttggccagc gagcagaagt cgccgcccgg gcagcagatg atgtcggtca gcaggccgat
      121 gttcggcgtg gccaggccca gctcgcgcag ctcgttccac agggcgaaca gctgagcttg
      181 ctcgacgtcg gcgaggatga tgttctgctc gtggctggtg cgcgcctcgc cgaagctgta
      241 gcgctcggcc aggtcggcga tggcgtcgaa ctgcttatcg gtgacgtcgc cgggggcgat
      301 gccggtcggc ttgagcgaca gggtcacggc ggcgtagccc ggcttcttgt gggcgaccac
      361 gttgcgcgag cgccagcggg cgaagcccgg atgctcggcg tccagggcgg cgagggcggc
      421 gccctggtct tccagggcct ggtaggccgg gtcgacgaag tgctgggcga cgcgctgcag
      481 ctcggcctcg gtcagggtgc tcgggccgtc cttgaggtgc gcccactcgg cggcgacgcg
      541 ctcggcgaac acttccgggg tcagcgcctt gaccaagatc ttgatgcgcg ccttgtactt
      601 gttgtcacgg cgaccgtaac ggttgtacac gcgcaggatg gcttccaggt aggtgatcag
      661 gtgttgccag ggcaggaatt cgttgatgaa gctgccgacg atcggggtac ggccgaggcc
      721 accgccgacc agcacgcgga agcccagctc gccggcttcg ttgcgtaccg gctccaggcc
      781 gatgtcgtgg acttcgatgg cggcacggtc ggccgcggag ccgttcacgg cgatcttgaa
      841 cttgcgcggc aggtgggcga attccgggtg gaaggtggcc cactggcgga tgatctcgca
      901 ccacggacgc gggtcgatca cctcgtcggc agcgacgccg gcgaactggt cggtggtgac
      961 gttgcggatg cagttgccgc tggtctggat ggcgtgcatc tgcacgctgg ccagctcggc
     1021 gagcatgtcc ggcacgtctt ccagcttcgg ccagttgtac tgcacgttct ggcgggtgct
     1081 gatgtgcgcg tagttcttgt cgtagtcgcg ggccagcttg gcgagcatgc gcaactgcgg
     1141 agcggacagc aggccgtagg gcacgcagac gcgcagcatg ggcgcgtggc gctggacgta
     1201 gaggccgttc tgcaggcgca gcgggcggaa ttcctcttcg gccagttcgc cggcgaggaa
     1261 gcggcgggtc tggtcgcgga actgcgcgac gcgctcctcg atgatccgtt gatcgtactc
     1321 gtcgtatacg tacatgtaat tctctgctct caggctgtca gcgaaccccg gcggctgtgg
     1381 ccggcccgca tggtgcatct gcggcgcgca cggctgcgca ctccgctgga gtgagggcac
     1441 cttagcagct cgtttttatg caagaaagtg aagaataact atatgtacat aaccaaaatc
     1501 gataaggttt cgaggcgctc cgcgcaggcc aggccgcgat gagggttcag ctgagcgaag
     1561 cgtcgcttgg ctggcgctcg gacggggcaa ccacgacgag gttgcggccg ctgcgcttgg
     1621 cggtatacag cgcctgatcg gccatttcca gtacgcggtc gatggcggcg gcgctgtccg
     1681 gccagtgggc aacgccgatc gacagggtga ccggaccaca gggcagggcc gtgcaggttt
     1741 ccatgcgcag gcgcaggcgc tcggccacct gggttgcggt cgcgaggtcg acatccggca
     1801 gcaggatgag gaactcctcg ccgccgacgc ggcagagcac gtcaccgccg cgcgagcttt
     1861 cgcgcatcag gcgggccaga tgctgcagct gcaggtcgcc gaccgcgtgg ccatggttgt
     1921 cgttgacctg cttgaagtgg tcgatatcga gggcaacggc ggcaaagggt tgtgtgcgag
     1981 cttgccactg ctccagggtc agttgcaggc cgcggcggtt gtagaggccg gtgagcggat
     2041 cggtgagggt gtcgagattg agcttgccga tcttccggtc gaactgggcc aggccggcga
     2101 tcagcgcgcg cttgagttcg gccgcctcga agtaccaggc gcccacctgg cggatttctc
     2161 cgggggcggt ctgcgagtcc atctgccgcg cactgctggc cagctgccac agcggctggg
     2221 cgatcaggcg cgccagccac cagatgccca gcagggagag cagcgagaac ggcagcgcat
     2281 tgcgcaggat gctcagcatc aggccgtcca gctccgccag ggtattggct gttgggcgct
     2341 gggcgacgat accccagccg gtactggcga cgggggcata gccggcgagc atgtcgatgt
     2401 cgcgcgagtt gacgatctgc tggctgcccg cctggccggc gatgacggct gcgacggccg
     2461 ggttgctgtg gatgggctcg cccacccgct cggcatcccg gtgatagagc agttgtccgt
     2521 gctggtcgac gacatagata taggagccgt cgcggtagaa gtgctcgccg agcaggctgt
     2581 tgaggatgct cctttcgcgc agatagatgc tgccgccgac atagccgagg taacgcccgt
     2641 ctgcggcgat gatcggctgc gagatcatca ccaccagctt gccggcggtg gagacgaacg
     2701 gtgcgctgac cagtggctgg cgcttttcca gggactccct ggcccccgcg gagttgagca
     2761 ggctcccggc aggaagcagc atcgctgggg aaatggccag cagcgtgagg tcgtcgcgca
     2821 cgatgtaggc ggagttgaaa gtgtcggttt gctgcttgag tcgttccgtt tccgcggcca
     2881 gctgctgcgg ctgctcgagg agggcgggca gtcgcgctgc gctataggcc agctgctggc
     2941 gcgcggaggc gagcaggccc tcggtgcttt ccgccagttt ggccgcatac acgcggttgg
     3001 cttccagggt ctgctggatc aacaagtcgc gctgcacctg atagccgacg tagaagctgt
     3061 tgatcagggt gagcagggcg gcggtgatgc tcagcagcag gatcaggcgg cgcagatcga
     3121 ctctgaacat gggagagaag cgcatgttgc gacgccaaaa aattcagtta tgtgaactat
     3181 ataacaaact actggcgcat ggctcatgct cgaagcgtcc gcaaaaaaag agatgcgcga
     3241 acgatgccgg gttgccttga gtttgcccgg gggctgggct tgactgcaga taggcacggc
     3301 agaggagagt cgcgatgaac cagcaggtca agacgcaagt agccagcatt gcagacagtc
     3361 gggcggatgc ctgggcggcc ctgagcctga tcatgatcgg ggtgacgacg gtcatcctgt
     3421 gggtgagcca gcaatgaccg gcggggtgca gaccccgcac tttccccccg cctgagcggc
     3481 cggcggctac ttcacgccgc tcagttgcag caccaggcgg accacgctgt acagcagcag
     3541 gatgaagacc ccggtgaaca gcaggccgag caggatgaaa tggctgggct tgccacggga
     3601 gaagtcacgc gcccggtttt taccgctctg cacgccaaaa gccgcagcca gcacgctctg
     3661 cagcatttgc cggaaggtga ggggggactc gtcctggtcg tcgttgcgtt ggggctgctg
     3721 ctcgctcatg gccggctcca cgggggggga tgctgcaagc tttgtccgca gaccgtcgtt
     3781 gtgcaagccg cggctcgcta gatttcgatg cgtgcccagg cgcggcgttg ccacatcagc
     3841 acgaaaatcg ccgatgccag gccgcgctgc aggatgtaca ggctccagat cgccagcagg
     3901 ccatagccca gcacggggcc gagcaggtag gtcagcggca gcaccaccag ccactggttg
     3961 ccgagggtca ccagcatcac cgtgcggctg gcgccggcgc cgagcagggc ctgggcgagg
     4021 accagcgcgc cggcatccag caccatgccc agcccggtca ggcgcaacgg ccaggtgccc
     4081 agctcgatca gttgcggggc gctgacgaac aggccgagaa tcgcctcggg aaacagccag
     4141 aacggtgtcc ccagcagggc caggccgccg atggcgaccc gcaccacgtc ccagccccag
     4201 cggtgggcgg cgtcgacatc gccggcgccg aggctgtggc tgaccagggt ggtggcggcc
     4261 atgcccaggc cgaccgccgg caggatcagc agcagcgcca gattcaccag cacatgcgcc
     4321 accgccagct cggcggtgcc gatctgggcg atgatccaga acagcagggt gatgccggtg
     4381 gcgaagaaca gttgctgcag cgagttgggc agtgacaggc gcgacagtcg ctggatctcg
     4441 accggcggcg ggcgctgcca gcggaagccg gccgccggtg ccacccgcca ggtctggctg
     4501 gcgaaaatca gcgagccggc gtactgggcg atgcaggtgc ccagtcccgc gccttgcgca
     4561 cccatggccg gcaggccgag caggccatgc accaggcaat agctgagcgc ggcattgaac
     4621 aggtggatgc ccagcaggag gcgcaggtac aacccggaac ggtggctgcc gttccagtag
     4681 ccgcgaaagg cgaagttcat ccccaccgcg gccaccgcca gcacccgcca ctggaaatag
     4741 tcgctgccga tggcggcgac ggcgggatcg ttgctgagca ggccgatgat ccacggcgcc
     4801 gccagccagc cgcccagccc cagcggcagg ccgaccagca cggccagcag cagcccggca
     4861 gtcaatggcc caccggtttc cgccaggcgt ccttcgccgc ggcgccgcgc caccagcgcc
     4921 tgcaccccgg agccgaggcc catgaccagg gcgatggcca tgaagttcgc ataactgccg
     4981 atgcccacgc cggccagtgc ggtttcgccc agatggccga ccatggccgc atccaccaga
     5041 ttgatcaggc tctggctgag catgccgccg atgatgggca ggccgaggat caggatctgg
     5101 cgcaggcgct ggggcattgg cagtgggtct cgggcagggg cgcgggcatt ctaacccgga
     5161 aatccggggc tgcaggctgc ccccggatgc cggtgtacaa tgcctgttac agggctgtgc
     5221 caagggccag tccgtggttt tacatcgccg tgtcacgtgc gtggcgacct tacgatacgg
     5281 cagcaaaagc catgccgaca ggtgattgat gacccacgaa ccccagcatc cgggcgagct
     5341 ccagcgcagc ctgaagaacc gccacatcca gctgatcgcc ctgggcggcg ccatcggcac
     5401 cggccttttc ctcggctcgg ccggggtgct gcagagcgcg ggaccgtcga tgatcctcgg
     5461 ctatgccatc gccggctgga ttgccttcct gatcatgcgc cagctcggtg agatgatcgt
     5521 cgaggagccg gtggccggct cgttcagcca cttcgcgcac aggtactggg gcggcttcgc
     5581 cggctttctc tccggctgga attactgggc gctgtacatc ctggtgggca tggccgagct
     5641 gaccgcggtg ggcaagtaca tccagttctg gtggccggac gtgccgacct gggccacggc
     5701 ggcggcgttc ttcgtgctga tcaacgcgat caacctgacc aacgtgaagt tcttcggcga
     5761 ggcggagttc tggttcgcca tcatcaaggt ggtggcggtg ctcggcatga tcgtgctcgg
     5821 cctgtggctg ctgctcagcg gcaccggcgg cgagcaggcg caggtcagca acctgtgggc
     5881 gcacggcggc ttcttccccc acggcgtcgg cgggctggtg atggcgctgg cgttcatcat
     5941 gttctcgttc ggcggcctgg agctggtggg catcaccgcg gccgaagcgg ccgagccgaa
     6001 gaaggtgatc cccaaggcga tcaaccaggt cgtctatcgc atcctgatct tctacgtcgg
     6061 cgccctgggc gtgctgctct gcctgtatcc ctgggatgcg ctgctggccg ccctcaacgc
     6121 ctccggcgac ccctacagca gcagcccgtt cgtgcagatc ttctcgctgc tcggcagtga
     6181 ggtggcggcc aacatcctca acttcgtggt gctgaccgcg gcgctgtcgg tctacaacag
     6241 cggcgtctac tgcaacagcc ggatgctcta tggcctggcc gagcagggca atgcaccgcg
     6301 cgcgttcctt aggatcgaca agcgcggcgt gccggtgatc gccatcggcg cctcggcgtt
     6361 gatcaccctg ctgtgcgtgc tgatcaacta caccctgccc aagggggcgc tggagctgct
     6421 gatgtcgctg gtggtggcgg cgctggtgat caactgggcg atgatcagtc tgatccacct
     6481 caagttccgc gccgccaagc agcgcgaggg cgtgcagctg gagttcaagg cgctgtggta
     6541 tccgttcggc aactacctgt gcctggcctt cgtgctgctg atcctcggcg tgatgtggca
     6601 gctcgagggc attcgcctgt cggtgtacgc gatcccgctg tggctgctgt tcatcggcct
     6661 gtgctaccgg ctcaaaggtg cgttcggtgc ggccccgagc ccgcgcatag tccgctgagc
     6721 gaggctgcgc ggcgcgccag cccgcagggc aatcgcatga acggtgtagg agcgaattta
     6781 ttcgcgacct tggccgcgca atcgcgaata aattcgctcc tacagtaagg aatacagcgg
     6841 attcacccca cgcatggtgg ttttttcagc ttcgtgcgat tgtcctgggt ccgcccgaac
     6901 ggtggctacg ccgtcttcgc cagctgatcg gtgcgggccg ccatgatgaa gtcgttgcgg
     6961 tgcagcccgc cgatcttgtg cgtccaccag gtcaccgtga ccttgcccca ttcggtgagc
     7021 agcgccgggt gatggccctc cacctcggcg atggcagcga ccgcattggt gaagttcaac
     7081 gcccgctgga agtcgggaaa gctgaagact ttctccagct gcatgacgcc gtcgcgcacc
     7141 tcgatgttcc agtccgggat ctggcggatc agctcggcca gttcggcgtc ggagacctgc
     7201 ggcgcgccag cgcggcaggc ttcgcagtgg gcttgggaca gggggttcat ggcgggcacc
     7261 tcgacgtttg ctgattgcat gagtgtaggg tgctctcgcc aggttgccgt cgtctgccca
     7321 tgcaggccgt cagatggccg cgaggcaggc gtcgaggctg gcgaagtccg ggcgctgctc
     7381 gataggttcg gtcacgcact gctcctgcag ttggcgcagg cgctgctgcc ggccgatctg
     7441 ttcgtccgcc gggcagcgct gcagcagctc ctcgagcaga tagccgaagg cacgcgcctc
     7501 gatgcgttgc agggcctggc cgctggcact gcccggatcg aagaacgagg cggcgccgaa
     7561 gtcgccgagc aggcagtcgc ccgccgcatt gaccagcagg ttgtgggcgt acaggtcgcc
     7621 atgcaggatg ccgcgctcgt gcaggtgcgc caccgccgat gccacgccgc gggcgatgtg
     7681 cagggcctcg tcgacgccga agcgccgctc cgtggcgtag cagtcgcggg tgcagctggc
     7741 cagcgacggc ggcccggcca ggggctggaa ggtcggggcg atcagctcca gcaacagccc
     7801 cggcgcggtg tctggctgcg cggccagcgg cccggccacg cggatcaggt tggcatgctc
     7861 gccagccgcg atgcaggcgg ccatttcgct gtgcgccagg ccgtcgctgg tcacattgcc
     7921 cttgaacagc ttggccgcca tggcctgtgg cggctgggcg ggctggtgcc actcggcgcg
     7981 gtggatgacc ccggaggcgc cctggcccag cacttcctgc agggcgatgc tgctgcgcgc
     8041 aatgggcggc agcggatgct cggccagtgt ctgcgcctcg cggctgtcgc tgaacgggtt
     8101 gccggcaaag gccagccagg ccaggcgcgg caattccgtc agccagccgg gcagggcgtc
     8161 gaggcggttg gcggcgatgc gcagcaattc caggttctgg caatccgcca ggctggccgg
     8221 cagcgcgtcg agccggttgc cggccagcgc cagtttttgc aggcgccggc actcgcccag
     8281 ttgctcgggc agtcgctgca gccggttgtc ggtgaggatc agccagcgca gcagcggcgg
     8341 cagggcggcg gctggcacct gctgcacctg gcaggacttg aagccgacca tctccagtcg
     8401 ggcgcagtcg ccaagcactt ccggcaccgt ggtgaacgga ttgtcagagc agaacaggat
     8461 gcgcagtttg cgcaggcgcg gcagctctgc cggcagctgg ctcagctggt tgccggagag
     8521 gttgagcacc tccaggctgt cggcgagatc gaagatctcc tcgggtaact ccgtcagccc
     8581 ggcagacagg tccaggcgcc ggatgccggc cagttcgccg cgacgcagtt gttcgagagt
     8641 atgcatggcg aattcagact gcccgctcga agtaggtttg cgtggcggtg atgcgtgcca
     8701 cctcttcgac ataggcgcgc accgccggcg ggaaggccag gcccttgacc atgctcaggt
     8761 tgcgcaggcc ggggtagagc agcacgtcgt cccagcccag ggtgttgcca tgggcgctgg
     8821 gcaggtcgag tggcggcagg ctggccagca tctgctcgac cacggcccgg tgctcggcgg
     8881 tttccgccag ggcctgggcg aaggggcgct ggatgatctg ctccttcttg gcctggaagt
     8941 agtcgcgcgc cgactgggtg gcgaactcgg gcaggccgag ggcgatgtcg cgggggaaca
     9001 gcaggcacca gttcgccagg cgtacctggt tcatgtgctc ctggatcgcc aggtagtcgc
     9061 cgtgcgggcg gatcacctgt gccgggttgc ccagctcgtc cagcttgcgg gcgatgtcca
     9121 ggctctcgcc catggcgcgg ccatcgtcga actgcaggat cggcacctgc ttggcaccga
     9181 tcagcgcgaa gcagctcgcc tcgtcgtcgt tgagcaggta gacctgctcg accggcacct
     9241 gcttgtagtg ggcgaccatc tcggcgcgca cgcagaacgg gcagtggtcg tacagataga
     9301 gcttcatgca gcctccttgg tctgggcagg ctgcagagtt taaccgttca atccgggctt
     9361 cagcctctct atgttgtgct tgatgacctc tttcaccgcc gcggtcatta ccccggtgga
     9421 gatcccgagc atgagcacgc cgttgccggc ctccagcgcg ccaagcaggc gccagggttt
     9481 ggagaggacg atgtcgccat agccgagggt ggcgaaggtc acccccgagt agtagatcgc
     9541 cgtggcgaac tcgtcgaact cgccgagcag catgaacagg ccggcccaca gggtgatctg
     9601 cacgaagtag ccaagcatca tcagcagcat cacaacgccg agcaccagga gatcccacag
     9661 gatggccttg ttgcccgccc cattgccgct ggggcgggtg tggtagccca cgtagtagcg
     9721 caggcatagg accacgatgt acgcctccat gagcaggcag agcagcatgg tgggaaggcc
     9781 gacgagcata ttgagcagca tggggatctc ctcagcagcc gggctgccgg atggcgcgac
     9841 gttggcggta ttggtcgagc agatagacca tcagccaggc atacagactg atggcgacga
     9901 acagcgccat gcgcacgacg aaggcgctgt agacatcctt gccgttggcg ctgtcctgca
     9961 ccgacaggcc gagcaggatg atcatggtca ccaggctgtt cagccagaag ccggggcttt
    10021 gccgggtcgg gctgaagccg tagagcttgc gcgccaccag caggccgaac agcagcatcc
    10081 acaggaaaaa catccacagg tgcacgaaca ggcccagcgc ccaccagaac agcatggcca
    10141 gcaggccgcc gagcaaggtc gagccgatca gctcgcgcgc ggcatcgcgc accgttgccg
    10201 tgcagctctg ctgaccgagg ccgaccgact tgaggatgat cgccatgtag ctggccgggt
    10261 cgatcagcgc cagcaggtag gccggcatca ccaccagggc agcgcgcaac gccacccagc
    10321 cggtctcttg cgcaggcagg gccggcgcgg ctggcggcgc ctgcgccccg gctggctcgg
    10381 gaaacagcca atgactgacg ggcaacatca gcacggcgag cagcaggccc tgcacgaggg
    10441 cgctgatgac cttgagcgcc agggcgaatt cggcggtccc ggcggccgaa atcatcgtca
    10501 ggccgaccac cagaaaggtg gtgaccagac tgttgccgcc gctcaggcca tggcgaaaag
    10561 ccaggaacag gcacaggccg accagcagca cgccgctgac gggggcgtag cgcagcaacg
    10621 ggatcagcag caggccgctg ccggtggtga gcatcaccac cagggtcagg cccagcccgg
    10681 ccttgaacga cagcggttga ttgagcttgg cgagcaggaa cagggcgaat atgggagcaa
    10741 tgaaggacag ctccagcccc aggccgtagc tgaccgtcag acacagcgca acaccgctgg
    10801 ccagacgcag cgcacgctgg gcgcggggct cacgcggggt ggccatggcc gcactcagta
    10861 ggcataggac agccagctca tcagatgcac gaacaggcga ccgagcggat tgagcggatt
    10921 gccctcggtg gggaacgcca tgacatcggc ctggccgccc acgcggatgc cgcgcagctt
    10981 ggccaaccgg gccgcctcgc ccgcggcgaa ctcgacgatc accgggaagc gctgcgccgg
    11041 gcgcagccag tcgcggctgt tctccacgct gggcaggctg ccgggggcgg tgctctgccc
    11101 gacgctgacg ccgtagccga cgctgcgtac ccgaccttcg aacacctcgc caggcaggac
//
LOCUS       DATCUN010000055        33219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_246262, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000055 DATCUN010000000
VERSION     DATCUN010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 33219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33219
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246262"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VIO83_01895"
     CDS             complement(<1..184)
                     /locus_tag="VIO83_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003180624.1"
                     /note="with YejAEF is involved in resistance to microcin
                     C; Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin ABC transporter permease"
                     /protein_id="HEY8330072.1"
                     /translation="MLAYIFRRLLLIVPTLFGILLINFIIIQAAPGGPVEQMIAKLEG
                     FDAAAGGATGRIAGGGA"
     gene            complement(185..2017)
                     /locus_tag="VIO83_01900"
     CDS             complement(185..2017)
                     /locus_tag="VIO83_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8330073.1"
                     /translation="MTVFQRCTRLFAGGVLMLAVAVNATAAARHALTLYDEPPKYPAD
                     FQHFAYVNPDAPKGGTLRQAGFGGFDSLNPFINKGVAADEISLLYDTLTRRSLDEPFT
                     EYGLLAQSIEKAPDNRWVRFTLRPEARFHDGQPVTAADVVFTFETLMKDGAPQYRAYY
                     ADVEKAVAEGERRVRFDFRHNGNRELPLIVGQLPVLPKHAWAGREFARTSLDAPLGSG
                     PYQVAEVQAGRSIRYERVKDWWAKDLPVNRGQYNFDSLTVDYYRDNTVALEALKAGQF
                     DFWVESSAKNWATAYDSPAIHDGRLIKEEIANHNPTGMQGFIFNLRRPQFQDARVREA
                     LGLLFDFEWTNRQLFHSAYVRTRSYFENSELAASGLPSPAELKLLEPWRNRLPPQMFS
                     AEFQPPYSDGSGVLRDQRRRAFQLLQEAGWRLEQDRMVDASGKPVTIEFLLAQVEFER
                     VLLPFKRNLAGLGIELQIRRADISQYINRLRSRDFDLIVGGFAQSSSPGNEQREYWHS
                     SSADNPGSRNLIGLKDPAIDALVEGLIKADSRESLIAHTRALDRVLQWGYYVIPNWHI
                     KTWRVARWNRFERPAVAPLYDIGLYTWWTKPGEQSAPVAAGESR"
     gene            complement(2014..3843)
                     /locus_tag="VIO83_01905"
     CDS             complement(2014..3843)
                     /locus_tag="VIO83_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8330074.1"
                     /translation="MSRLLALLLGLAACLPTSAAVTERHGYAQFGALKYPANFSHFDW
                     VNPDAPKGGTVRLMASGTFDTLNPYTLKGTSPAGTGNFLQYGVSELNAPLMAGTGAYD
                     PSGDEPTSSYGLIASSVEYSKDRSWVVFNLRPEARFHDDKPITAYDVAFSYRLLSRDG
                     HPQYRTALQEIQRVDILNRHRVRFVFKRSGNPLLILRLGELPVLPQHYWSKRDFKATS
                     YEPPLGSGPYRIAQVAPGRRVVFERVKDWWGKDLPVNRGKYNFDRVEVEFYRDNHVAF
                     EAFKAGEFDFYIENQAKNWANGYNIPAVERGDIVKAEIPHQIPTQTQALFMNSRRAHF
                     ADARVREAMGLLFDFEWSNRTLFNNAYSRATSYYPNSEFSARDIPQGEEWLLLSPFRE
                     QLPAELFSAAPQIPQTDGSGLPRETLRRALDLLAQAGWKSAGGRLLNSRGQPLRFEIL
                     LVNPSLERILQPYRDNLARIGVEASLRTVDRAQYKQRIDGFDYDMVLMTLPQTLSPGL
                     EQWQYFHSSQVHVRGSKNYAGISHPVVDALLDKLLAAQTRDQQVAAVRALDRVLLSQH
                     YSIPNWYSDHHRLAWRNRFDHVSTPPYTLGLRAWWLKTPEASR"
     gene            complement(3947..5524)
                     /locus_tag="VIO83_01910"
     CDS             complement(3947..5524)
                     /locus_tag="VIO83_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010794048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY8330075.1"
                     /translation="MPPPTPQSSVRTAASRALQLLLVGLLVVVAGCQSRGGAEPENTV
                     RASRQIEWTKTPPPQEEQHGDIWERIRAGYKLQDQIGTNPRIERQRFGFASHPASIQR
                     TAERSGPYIHYIVERLEENDMPLELALLPMIESSYNPMAYSPAHAAGLWQFIPSTGRH
                     YNLRQTDWYDGRRDITASTNAAINYLKRLHDMFNGDWLLALAAYNAGEGTVSRAIERN
                     QSRNLPTDYWNLPLPRETQDYVPKLLAVSQIIQAPTSYGVALAPIANQPYFEKVAIRH
                     QLDLSRVAAMADVDKDELLQLNPAFKQGITLDGPRHLLVPVAKADGLSESLARLQPDS
                     LMQWQRYQVRRGDSLDSIAKRHGVASSTLRDLNQLPDRQVRTGQALLIPQLPGNAVQE
                     ERSAPVVAARAETTTKPALRRYRVRKGDTLWSVAKQHNVAVADLQRWSKLKGKSIHAG
                     QVLTLHEDRPRAAAKAKSGGDTRQSVTYYKVRRGDSLSDIAQRFAVGTHHLKQWNPSL
                     GRTLKPGQTLTLYLGRR"
     gene            complement(5601..6380)
                     /gene="gloB"
                     /locus_tag="VIO83_01915"
     CDS             complement(5601..6380)
                     /gene="gloB"
                     /locus_tag="VIO83_01915"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014603047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEY8330076.1"
                     /translation="MIQITALPAFSDNYIWLLQDAAQRRCAVVDPGDSAPVLAWLATH
                     ADWQLTDILVTHHHHDHTGGILALKEHTGARVLGPALEAIPGRDVALEDNQRIRLLDH
                     DWQVLHVPGHTAGHIALFGGDDVQPLLFCGDTLFSAGCGRLFEGTAAQMHASLQRLAA
                     LPAQTRVYCTHEYTLSNLRFALAVEPDNPALHQRAAEAASLREQGLPTLPSTLAIERA
                     TNPFLRVNEAGVRQQGEQRAGHSLDSPSAVFAELRSWKDNF"
     gene            6458..7222
                     /locus_tag="VIO83_01920"
     CDS             6458..7222
                     /locus_tag="VIO83_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8330077.1"
                     /translation="MNEQPCGNAASDWLALQREAQAWFAGPLGRLLLDSQQALLEQQL
                     GSHFGSFLLHYGFPGGDLPPQRQITHYVRLGPPLPGVEIVCEEWAWPVLEHAADLVVL
                     QNALDFAASPHQLLREAARCVRPGGHLLIVGIHPWSLWGARHLLASDALAQARCISPA
                     RLADWLHLLGFALEKRRFGCYCPPLSSQRWQARLGAIAPWAERLQLPGGGFYLLAARK
                     LVAGLRPLPQPRRAAVALLRPVPVAKVGRHPGKKES"
     gene            7219..7674
                     /gene="rnhA"
                     /locus_tag="VIO83_01925"
     CDS             7219..7674
                     /gene="rnhA"
                     /locus_tag="VIO83_01925"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016979720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY8330078.1"
                     /translation="MTDSVEIYTDGACKGNPGPGGWGALLIYKGVERELWGGESPTTN
                     NRMELTAAIRALETLNRHCRVQLTTDSEYVMKGIKEWLPNWKKRGWKTAARQPVKNAD
                     LWQALDEQVNRHEVHWHWVRGHNGHPGNERADQLANRGVGEVNGSRQSV"
     gene            7725..8486
                     /gene="dnaQ"
                     /locus_tag="VIO83_01930"
     CDS             7725..8486
                     /gene="dnaQ"
                     /locus_tag="VIO83_01930"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020288765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEY8330079.1"
                     /translation="MRYVVLDTETTGLEAREGHRIIEIGCVEVIGRRLTGRHFHVYIN
                     PEREVDEGALAVHGISTEFLADKPRFGEVVDEFFEFIKGAELIIHNAAFDIGFINHEF
                     ARLKDRPQLGEVLDHCSVLDTLLMARERHPGQRNSLDALCKRYGVDNSGRELHGALLD
                     AEILADVYLAMTGGQTSLSLAGQNGEGEGGNGPQVAEIRRLPADRPLTRVIRASEAEL
                     ELHAARLAAVAKAAGGPALWQQLEQAAEDVASTLH"
     gene            8567..10828
                     /locus_tag="VIO83_01935"
     CDS             8567..10828
                     /locus_tag="VIO83_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8330080.1"
                     /translation="MYKDLRFPVLIVHRDIKADSVAGERVRGIIHELEQDGFTILSTA
                     SSREGRIVASSQHGLACILVAAEGAGDNPRLLQDVVELIRAARVRAPQLPIFALGEQI
                     TIENAPPEAMAELNQLRGILYLFEDTVPFLARQVARAAHNYLDGLLPPFFRALVQHTA
                     ESNYSWHTPGHGGGVAHRKSPVGQAFHQFFGENTLRSDLSVSVPELGSLLDHTGPLAE
                     AERRAARNFGADHTFFVINGTSTANKIVWQAMVGRDDLVLVDRNCHKSVVHALIITGA
                     IPLYLNPARNELGIIGPIPLAEFSRESIAAKIAASPLARGREAKVKLAVVTNSTYDGL
                     CYNAELIKRELADSVEVLHFDEAWYAYAAFHEFYAGRYAMGTSRDEGGREPLVFSTHS
                     THKLLAAFSQASMIHVQDGGQRQLDVALFNEAFMMHTSTSPQYGILASLDVASAMMEG
                     AAGRSLIQEAFDEALAFRRAMANLRRNLPAADWWFDIWQPERGEAALQVQTSDWLLAP
                     QAPWHGFGEVAEDYVLLDPVKVTLVMPGLFADGRLDREGIPAAVVGKFLWERGVVVEK
                     TGLYSLLVLFSLSITKGKWSTLLTELLEFKRLYDGNAPLGEALPSIAHAKPARYAGMG
                     LRDLCQQLHACYRDNAVPLALRRMYTRLPEAVLKPADAYDQLVRGAVEAVPLDALEGR
                     IAAVMLVPYPPGIPLIMPGERFTEDSRPLIDYLTFTGRFERQFPGFAADVHGLQRRGG
                     AKGKWHTVDCIRG"
     gene            11088..12776
                     /locus_tag="VIO83_01940"
     CDS             11088..12776
                     /locus_tag="VIO83_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEY8330081.1"
                     /translation="MVSGATSLGLVRSELFATIEQAEQSLEQFIAERHNVSLLQQAVE
                     YLQQIRGILKLIELVGAELLAQEVLLLATDIPAGAGQERDGQLSALGKALFVLRRYLE
                     GLDNHRQEIPELLLPMINELRQSTGQPSLPESYFFSVRLDQPRPARRNVEIQPGVANR
                     DGTRLRQMYQLGLMNVLREQNLGAALKLMSRSLSRLDDLLGARERSRLCWIGAAALEA
                     MAEAQLRPTKTRKQLLARIDRDLRQLLSNPQFEAPRSLLKELLYLVALAGSQGERASE
                     MRRVFGLASLPFTDHLLDEESRRLSGPGQEVMRSLSLAIREELVAVKDILDLIERGAA
                     QVESKVELHAQIGKLAKTLGMIGLSSAANALQAQVATVGSWVERSDNPAAAELLRLAD
                     ALLYVESMVATLEQGDQRQRQVNVAQANGQGDGESFATHQLFEAHVVVMDEAQAGLAL
                     AKRAITAYLESNGDKLNLANVPTSLQAVRGGLWFLGLARAAELVGACADYIQKHMIEI
                     TQMPSEQMLETLADALTSLEFFLEGGANLRPEGQQDVLDLAADSVRALGMQVAA"
     gene            12776..13615
                     /gene="nudC"
                     /locus_tag="VIO83_01945"
     CDS             12776..13615
                     /gene="nudC"
                     /locus_tag="VIO83_01945"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEY8330082.1"
                     /translation="MGGDLWQPAVIDPAQAGGWAVAHCHQRFLGDSSGVLFPREWLKA
                     RLPAALAEHGIGYFQGQSVFVLELAEPVDGLLPGGQWKGLRQFLLEGDISTYRMLGYA
                     AQIGTWARQHRFCGSCGGPLQPLSGERAMLCPACDLHLYPRLSPSIIVLISRGEELLL
                     ARSPRFAAGLYSTLAGFVEPGESIEECVRREVREEVALEVDNLRYIASQNWPFPHSLM
                     LGFHADYAGGEIVPQAEEIEDARWFRVDDLPQLPPHGAISRYLIELYLARRSGVAEPL
                     LPG"
     gene            13815..14462
                     /locus_tag="VIO83_01950"
     CDS             13815..14462
                     /locus_tag="VIO83_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003239994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330083.1"
                     /translation="MQYNGLAWATALAALMVLAIAGRILGNRSWFLGWLRGTCGLALL
                     ALALLAGVVAYDLHSYAPLPRQGQALLTLSFQAEGAQRYRATVREGGRERSVILSGDL
                     WQLDARVFDWKGLASLIGLQPGYRLELLAGRYLSAEQQAAAEPSRIALASSPYGIDVW
                     RWLREGRHDLFLFDARGARVTFLPMADGAVYSVSLGSAGLVAEPQTQAARAALSR"
     gene            complement(14577..16172)
                     /locus_tag="VIO83_01955"
     CDS             complement(14577..16172)
                     /locus_tag="VIO83_01955"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEY8330084.1"
                     /translation="MSAYQNDIKAIAALKEQFGSSWSAINPESVARMRAQNRFKTGLE
                     IAQYTADIMRKDMAEYDADSSVYTQSLGCWHGFIGQQKLISIKKHLKTTNKRYLYLSG
                     WMVAALRSEFGPLPDQSMHEKTAVSNLIEELYTFLRQADSRELDLLFTALDAAREAGD
                     QAKAAEIQSQIDNYETHIVPIIADIDAGFGNPEATYLLAKRMIEAGACCIQIENQVSD
                     EKQCGHQDGKVTVPHADFLAKVAAVRYAFLELGIDNGVIVARTDSLGAGLTKQIAVTK
                     QPGDLGDQYNSFLDCEEITEAELGNGDVVIKREGKLLRPKRLPSNLFQFRKGTGEARC
                     VLDSITSLQNGADLLWIETEKPHVGQIAAMMDEVRKVIPNAKLVYNNSPSFNWTLNFR
                     QQVFDAMAAEGKDVSAYDRAKLMSVEYDETELAQIADEKIRTFQRDGSAHAGIFHHLI
                     TLPTYHTAALSTDNLAKGYFAEEGMLAYVKGVQRQELRQGIACVKHQNMAGSDIGDNH
                     KEYFAGEAALKASGKDNTMNQFS"
     gene            complement(16599..17411)
                     /locus_tag="VIO83_01960"
     CDS             complement(16599..17411)
                     /locus_tag="VIO83_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEY8330085.1"
                     /translation="MNLRPWLALLGLVCSLALQAAPRTEILALQYRTAEELLPLVQSV
                     LGHEGRVSAYGNQLIVNAEPAKLAELQELLQQLDTRPRRLLISVDSQQDSSSNRQGYR
                     VDGSIDAGNVEISGGRGEQHGRDELRIINRSSRGSSGSLQQVQATEGYPALIQTGQSV
                     PLTRIQRDAYGYPREYTEYRDLNRGFEVVASVHGEMVQLSIRSQQDRLGSRPGVIDTQ
                     DTDTRINGRLGEWIELGGVSESVSGNRSAILSQDARNARQNQALRLKVELLD"
     gene            complement(17408..18040)
                     /locus_tag="VIO83_01965"
     CDS             complement(17408..18040)
                     /locus_tag="VIO83_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase HPA2"
                     /protein_id="HEY8330086.1"
                     /translation="MSNAPEQANDDASELPAVEFRSPGRFAIHNPPAATREDAGHLEP
                     APFLLGAHAPVETCTAREQLRSHALALLGQARRDLCLYTPDLEPWLYDHPAIAEACSR
                     FLRGHPRNRLRILLRDSSLAVRNGHHLLRLARQLSSNCQIRKLHPDYPAEDLAYLLVD
                     DCGLLLRTAPLLPASQVHYQAAARVRQLRRQFDQAWNTSLGDPNLRSLLL"
     gene            complement(18050..18475)
                     /locus_tag="VIO83_01970"
     CDS             complement(18050..18475)
                     /locus_tag="VIO83_01970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8330087.1"
                     /translation="MNKISVRVADWHADNADLRRIREAVFIVEQGVSPELEWDADDTD
                     AVHFLAFEGDFAVGASRLLPDGQIGRVSVLKDWRGLHIGEALMQATIAEAERRDLKQQ
                     KLTAQAHAIRFYEQLGFRVASEEFLEAGIPHVEMVRDSA"
     gene            complement(18468..19637)
                     /locus_tag="VIO83_01975"
     CDS             complement(18468..19637)
                     /locus_tag="VIO83_01975"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8330088.1"
                     /translation="MAPDTPLQLLGGLTPREFLRDYWQQKPLLIRQAIPGYQSPISPD
                     ELAGLSLEEEVESRIVLEHGNSPWELRRGPFAEDAYQHLPERDWTLLVQAVDQFVPDM
                     RELMEQFLFLPSWRIDDLMISYAAPGGGVGPHFDNYDVFLLQAHGQRRWKLGQMCDSD
                     SPLLDHPDLRILADFEQTDEWVLEPGDMLYLPPRLAHYGIAEDECMTFSVGFRAPSAS
                     EVLTHFTDFLGQFLPDEERYSDAGCQPAGDPNQIQRDALERLRSLIDRHMSDEKLLLT
                     WFGQFMTEPRYPEMVAGEEIDEAEFFTALGDGAVLVRNPSARLAWSEVDDYLVLFASG
                     NSRRVDASLRDLLKLVCSADALHLENLEPWLESDEALTLIHELVKQGSLEFAADE"
     gene            complement(19731..21101)
                     /gene="purB"
                     /locus_tag="VIO83_01980"
     CDS             complement(19731..21101)
                     /gene="purB"
                     /locus_tag="VIO83_01980"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY8330089.1"
                     /translation="MQLSSLTAVSPVDGRYGSKTNALRPIFSEFGLIRCRVQVEVRWL
                     QRLAAHAGIPEVAPFSAEANALLNELAENFQLEHAQRIKDIERTTNHDVKAVEYLLKE
                     QAAKLPELAKVSEFIHFACTSEDINNLSHALMLREGRDDVLLPLMRQVAESIRTLAVQ
                     FADVPMLSRTHGQPASPTTMGKELANVVYRLERQIAQVAAVPLLGKINGAVGNYNAHL
                     SAYPEIDWEANAREFIEGDLGLAFNPYTTQIEPHDYIAELFDAIARFNTILIDFDRDV
                     WGYISLGYFKQKTVAGEIGSSTMPHKVNPIDFENSEGNLGIANALLQHLASKLPVSRW
                     QRDLTDSTVLRNLGVGLAHSLIAYEASLKGIGKLELNAQRLAADLDACWEVLAEPIQT
                     VMRRYGIENPYEKLKELTRGKGITPEALREFIDTLAIPEQAKSELRTMTPASYIGNAV
                     AQAKRI"
     gene            complement(21268..21888)
                     /gene="hflD"
                     /locus_tag="VIO83_01985"
     CDS             complement(21268..21888)
                     /gene="hflD"
                     /locus_tag="VIO83_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015648976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HEY8330090.1"
                     /translation="MTREQERLIALAGVFEAAALVDRLAKTGQAQEGPLACMLGSLLV
                     RNPKDTLEVYGGDHLNLRDGLRALAGALERNPGTLPRESLRYALSLLTLQRQLDKRGD
                     MLQVIGNRLQQIDSQSQMFGITHDNVISACAALYQDTLSTFRQRIQVHGEMQHLQNSR
                     NAEKIRALLLAGIRSAMLWHQLGGRRWQLLFSRRKLLDQLYPLLRD"
     gene            complement(21885..23012)
                     /gene="mnmA"
                     /locus_tag="VIO83_01990"
                     /pseudo
     CDS             complement(21885..23012)
                     /gene="mnmA"
                     /locus_tag="VIO83_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015636291.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
     gene            complement(23072..23521)
                     /locus_tag="VIO83_01995"
     CDS             complement(23072..23521)
                     /locus_tag="VIO83_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8330091.1"
                     /translation="MKRFIPHVTVATVVEDQGRFLLVEEHAEGRMVLNQPAGHLEANE
                     SLLQAALRETLEETGWEVELTGVTGIYLYTAPANGVTYQRVCFAARPLRHRPELPLDH
                     GIIGPRWLTREELEARHEQWRSDLVPRCIDDYLAGGSYPLELIRTPA"
     gene            complement(23627..25855)
                     /locus_tag="VIO83_02000"
     CDS             complement(23627..25855)
                     /locus_tag="VIO83_02000"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY8330092.1"
                     /translation="MSTRSKIIYTFTDEAPALATYSLLPIIQAFTASSGISVETRDIS
                     LAGRILASFPEYLNEDQKIGDHLAELGKLATTPEANIIKLPNISASVPQLKAAIKELQ
                     QQGYKLPDYPEAPASDTEKDVRGRYDKIKGSAVNPVLREGNSDRRAPLSVKNYARKHP
                     HKMGAWSADSKTHVAHMNSGDFYGTEKGALIEAAGSVKIELIAADGSSKVLKEKTAVL
                     AGEIIDASIMSQKALRSFVAAEIEDAKKQGVLLSAHLKATMMKVSDPIMFGYIVAEYY
                     KDALEKHADVLKEIGFNLNNGIGDLYARIKTLPAEKQAEIEADIQAVYAQRPPLAMVN
                     SDKGITNLHVPSDVIVDASMPAMIRDSGKMWGADGKLHDTKALIPDRCYAGVYQVVID
                     DCKKHGAFDPVTMGSVPNVGLMAQQAEEYGSHDKTFQIPSNGVVRVTDDKGQVLLEQA
                     VETGDIWRMCQAKDAPIQDWVKLAVNRARASNTPAVFWLDPARAHDALVIEKVQQYLK
                     DHDTSGLDIRIMSPEEATQFSIDRIRAGLDTISVTGNVLRDYLTDLFPIMELGTSAKM
                     LSIVPLMSGGGLFETGAGGSAPKHVQQFLEEGHLRWDSLGEFLALAASLEHVGTTCAN
                     AKAKVLAKTLDQANGQFLDSDKSPSRKVGQLDNRGSHFYLALYWAQALAAQTEDKELQ
                     AQFTNLAKALADNEEKIVAELNAAQGKPVDIGGYYHADTELTSQAMRPSATFNAALAA
                     LA"
     gene            complement(26283..26522)
                     /gene="cspD"
                     /locus_tag="VIO83_02005"
     CDS             complement(26283..26522)
                     /gene="cspD"
                     /locus_tag="VIO83_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008156 - negative regulation of DNA
                     replication [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HEY8330093.1"
                     /translation="MLSGKVKWFNNAKGYGFILAEGRDEELFAHYSAIQSDGYKTLKA
                     GQSVNFEILQGPKGLHAVNIQPLPVSTTPPRSSGY"
     gene            26748..27110
                     /gene="clpS"
                     /locus_tag="VIO83_02010"
     CDS             26748..27110
                     /gene="clpS"
                     /locus_tag="VIO83_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY8330094.1"
                     /translation="MHARSQIRLTFNQDSPDPSGGEDSGVAIQEAKPALKAPPMYKVL
                     LLNDDYTPMDFVVEVLESFFGMNRETATRIMLAVHTEGRAVCGVYTRDIAETKAMQVN
                     QYARECQHPLLCEIEKDE"
     gene            27152..29422
                     /gene="clpA"
                     /locus_tag="VIO83_02015"
     CDS             27152..29422
                     /gene="clpA"
                     /locus_tag="VIO83_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEY8330095.1"
                     /translation="MLNRELEVTLNLAFKEARTKRHEFMTVEHLLLALLDNGAAATVL
                     RACGANMDKLRHDLREFIDSTTPLIPLSEDERETQPTLGFQRVLQRAVFHVQSSGKRE
                     VTGANVLVAIFSEQESQAVFLLKQQSVARIDVVNYIAHGISKVPGESEPHEQEQESQD
                     EEGGESPVSGNPLDAYASNLNEQARQGRIDPLVGREPEVERVAQILARRRKNNPLLVG
                     EAGVGKTAIAEGLAKRIVDGQVPDLLANSVVYSLDLGALLAGTKYRGDFEKRFKALLN
                     ELRRRPHAILFIDEIHTIIGAGAASGGVMDASNLLKPLLSSGEMRCIGSTTFQEFRGI
                     FEKDRALARRFQKVDVLEPSVEDTVGILRGLKSRFEQHHKISYTDEALRAAAELAARY
                     INDRHMPDKAIDVIDEAGAYQRLQPESRRAACVDVPQIEDVVAKIARIPPKHVTTSDK
                     ELLRNLERDLKLTVFGQDAAIDALSTAIKLSRAGLKAPDKPVGSFLFAGPTGVGKTEV
                     ARQLAKALGVELVRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAITKTPHCVLL
                     LDEIEKAHPEVFNLLLQVMDHGTLTDNNGRKADFRSVILIMTTNAGAETAARASIGFT
                     LQDHSSDAMEVIRKSFTPEFRNRLDTIIQFGRLDHTIIKSVVDKFLTELQAQLEDKRV
                     LLDVSEAARGWLAERGYDAQMGARPMARLIQDKIKRPLAEQILFGELAEQGGTVHIDV
                     REGELVFDYETVALPA"
     gene            complement(29494..29712)
                     /gene="infA"
                     /locus_tag="VIO83_02020"
     CDS             complement(29494..29712)
                     /gene="infA"
                     /locus_tag="VIO83_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011217896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY8330096.1"
                     /translation="MSKEDSFEMEGTVVDTLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVRVELTPYDLSKGRITYRAR"
     gene            complement(29812..30519)
                     /locus_tag="VIO83_02025"
     CDS             complement(29812..30519)
                     /locus_tag="VIO83_02025"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011334947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEY8330097.1"
                     /translation="MTELAHLKFYATQPHDCSYLSGQQATTLFLDPRHPFDAGMYAGL
                     SELGFRRSGEHLYRPHCQHCQACLPARIPTASFQASQQQKRILKRNRDLSVHAVRPEF
                     REEYYDLYARYIEQRHADGDMYPPSREQFTTFLVSDLPFCVFFEFRLQGRLLAVAVTD
                     VLPNGLSAVYTFFDPLEERRSLGRVAILWQVAEASRRALPAVYLGYWIRNCRKMNYKS
                     EYRPLELYVNQHWVTLD"
     gene            complement(30572..31261)
                     /gene="aat"
                     /locus_tag="VIO83_02030"
     CDS             complement(30572..31261)
                     /gene="aat"
                     /locus_tag="VIO83_02030"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY8330098.1"
                     /translation="MLNWLARDSLKFPPLEQALREPDGLLAAGGDLSPERLISAYRHG
                     CFPWFQEGQPILWWSPDPRMVLFPAELHVSHSLRKLMRQGRFEVSFDRDFPAVIRACA
                     APRSYADGTWITRAMQAAYLELHRRGIAHSVEVWQEDQLVGGLYGLALGRLFFGESMF
                     SRTDNASKIGFVTLVEQLRECGYVLIDCQMHTQHLSSFGAREIPRADFAGYLERYLDQ
                     SGPTSWASTPA"
     gene            complement(31271..32218)
                     /gene="trxB"
                     /locus_tag="VIO83_02035"
     CDS             complement(31271..32218)
                     /gene="trxB"
                     /locus_tag="VIO83_02035"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY8330099.1"
                     /translation="MSDAKHSRLIILGSGPAGYTAAVYAARANLKPLLITGLQAGGQL
                     TTTTEVDNWPGDVEGLTGPALMERMREHAERFATDIVFDHIHTAELQQRPFVLKGDSG
                     TFTCDALIIATGASAQYLGLPSEEAFMGKGVSACATCDGFFYRNQVVCVIGGGNTAVE
                     EALYLSNIAKEVHLIHRRDKLRAEKILQDKLLEKAATGNIRLHWNQALDEVLGDASGV
                     TGVRLKDVNNGATQELPLTGVFIAIGHKPNTELFTDQLAMHDGYLKVKGGGEGNATAT
                     SIEGVFAAGDVADHVYRQAITSAGAGCMAALDAERFLEQ"
     gene            32431..>33219
                     /locus_tag="VIO83_02040"
     CDS             32431..>33219
                     /locus_tag="VIO83_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY8330100.1"
                     /translation="MKSSANTAKADALRQQLHYRLKEAALIALGALCIYFWMALLSYS
                     PMDPGIDVSTSAGHVQNAAGRVGAWVASFFLFGFGYSAYLLPLLMGIKVLELFRTRHE
                     PFRWNGWLFSWRIIGLFFLLTSTAALASIQFQALEMNPQAPAGGILGKSLGEWAEDAL
                     NVQGSTLMFIALFLFGLTVFTDLSWFRVMDLTGKITLDLFELIQGAGHRWWEGRQTHR
                     QLKLKLRESDEAVEPAAEPVSGRPRSAASAPAAPVARAVPAGVAA"
BASE COUNT         5237 a         9996 c        11729 g         6257 t
ORIGIN      
        1 cggcgccgcc gccggcgatc cgcccggtgg ccccgccggc ggcggcatcg aagccctcga
       61 gcttggcgat catctgctcc accgggccgc cgggagcggc ctggatgatg atgaagttga
      121 tcagcaggat gccgaacagg gtcggcacga tcagcagcag gcggcggaaa atgtaggcca
      181 gcattcagcg agactccccg gcggcgaccg gcgcgctctg ctcgcccggc ttggtccacc
      241 aggtgtacag gccgatgtcg tacagcggtg ccacggccgg tcgctcgaag cggttccagc
      301 gcgccacccg ccaggtcttg atgtgccagt tggggatcac gtagtacccc cattgcagca
      361 cgcggtcgag ggcgcgggta tgggcgatca ggctctcccg gctgtcggcc ttgatcaggc
      421 cttccaccag cgcgtcgatg gccggatcct tgaggccgat caggttgcgg ctgccggggt
      481 tgtcggcgct gctggagtgc cagtattcgc gctgctcgtt gcccggcgag ctggactggg
      541 cgaagccgcc gacgatcagg tcgaagtcgc gcgagcgcag gcggttgatg tactgcgaga
      601 tgtcggcgcg gcggatctgc agttcgatgc cgagaccggc caggttgcgc ttgaacggca
      661 gcagcacgcg ctcgaactcg acctgggcga ggagaaactc gatggtcacc ggcttgccgc
      721 tggcgtcgac catgcggtcc tgctccaggc gccagccggc ctcctgcaac agctggaagg
      781 cgcggcgccg ctggtcgcgc agcacgccgc tgccgtcgct gtacggcggc tggaattccg
      841 cgctgaacat ctgcggcggc agccggttgc gccacggttc gagcagcttc agctcggcgg
      901 gcgagggcag cccgctggcc gccagctcgg agttctcgaa gtagctgcgg gtgcgcacgt
      961 aggcgctgtg gaacagctgg cggttggtcc actcgaagtc gaacagcagg cccagcgcct
     1021 cgcgcacgcg ggcatcctgg aactgcgggc gacgcaggtt gaagatgaag ccctgcatgc
     1081 cggtggggtt gtggttggcg atctcctcct tgatcagccg gccgtcatga atggccgggc
     1141 tgtcataggc ggtggcccag ttcttcgcgc tgctttccac ccagaagtcg aactgcccgg
     1201 ctttgagggc ttcgagggcc acggtgttgt cgcggtagta gtcgaccgtc aggctgtcga
     1261 agttgtattg gccacggttt accggcaggt ccttggccca ccagtccttg acccgttcat
     1321 agcggatgct gcgcccggcc tgtacctcgg ccacctggta ggggccgctg ccgagcggcg
     1381 catccaggct ggtgcgggcg aattcgcggc ccgcccaggc gtgcttgggc agcaccggca
     1441 attggccgac gatcagcggc agttcgcggt tgccgttgtg gcggaagtcg aagcgcacgc
     1501 gccgctcgcc ttcggccacg gccttctcca cgtcggcgta gtaggcgcga tactgcggcg
     1561 cgccatcctt catcagggtc tcgaaggtga acaccacgtc ggcggcggtc accggctggc
     1621 catcgtggaa gcgcgcctcg gggcgcaggg tgaagcgtac ccagcggttg tccggcgcct
     1681 tctcgatgct ttgcgccagc aggccgtatt cggtgaaggg ctcgtcgaga ctgcggcggg
     1741 tcagggtgtc gtagagcagg ctgatctcgt cggcggccac gcccttgttg atgaacggat
     1801 tgaggctgtc gaaaccgccg aaaccggcct ggcgcagggt gccgcccttg ggcgcgtcgg
     1861 ggttgacgta ggcgaaatgc tggaagtcgg ccgggtactt cggcggctcg tcgtacaggg
     1921 tcagggcatg gcgggcggcc gccgtggcat tgactgccac ggcgagcatc aggacaccgc
     1981 cggcgaacag gcgggtgcag cgctggaaga cggtcatcgg gaagcctccg gggtcttgag
     2041 ccaccaggcg cgcaggccta gggtgtaggg cggcgtgctg acgtggtcga agcggttgcg
     2101 ccaggcgagc cggtggtggt cgctgtacca gttggggatg ctgtagtgct gtgacagcag
     2161 cacccggtcg agcgcgcgca cagcagcgac ctgctggtcg cgggtctgcg cggcgagcag
     2221 cttgtcgagc agcgcgtcga ccaccgggtg gctgatcccg gcgtagttct tgctgccgcg
     2281 cacgtggacc tggctggaat ggaagtattg ccactgctca aggccgggac tcagcgtctg
     2341 cggcagggtc atcagcacca tgtcgtagtc gaagccgtcg atgcgctgct tgtactgggc
     2401 gcgatcgacg gtgcgcaggc tggcctcgac gccgatgcgc gcgagattgt cgcggtacgg
     2461 ctggaggatg cgctccagac tcgggttgac cagcaggatc tcgaagcgca gcggctggcc
     2521 gcgactgttg agcaggcgcc cgccggcgga tttccagccg gcctgagcga gcaggtcgag
     2581 ggcgcggcgc agcgtctcgc gcggcaggcc gctgccatcg gtctgcggta tctgcggcgc
     2641 tgcgctgaac agttcggccg gcagctgctc gcggaagggc gagagcagca gccattcctc
     2701 gccctgcggg atgtcgcggg cggagaactc gctgttgggg tagtagctgg ttgcccggct
     2761 gtaggcattg ttgaacagcg tgcggttgct ccactcgaaa tcgaacagca ggcccatggc
     2821 ctcgcgtacc cgggcgtcgg cgaagtgggc acggcggctg ttcatgaaca gcgcctgggt
     2881 ctgggtcggg atctggtggg ggatctccgc cttgacgata tcgccgcgct ccaccgccgg
     2941 gatgttgtag ccgttggccc agttcttcgc ctggttctcg atgtagaaat cgaattcgcc
     3001 cgccttgaac gcttcgaagg cgacatggtt gtcgcggtag aactccacct cgacgcggtc
     3061 gaagttgtac ttgccgcggt tgaccggcag gtccttgccc caccagtcct tcacccgctc
     3121 gaacaccacg cgccggcccg gggccacctg ggcgatgcgg taggggccgc tgcccagcgg
     3181 cggctcgtag ctggtggcct tgaagtcgcg cttgctccag tagtgctgcg gcagcaccgg
     3241 cagctcgccg aggcgcagga tcagcagcgg attgccgctg cgcttgaaca cgaagcgcac
     3301 ccggtggcgg ttgaggatgt cgacccgctg gatttcctgg agggcggtgc ggtattgcgg
     3361 atggccatcg cggctgagca ggcggtagga gaaggccacg tcgtaggcgg tgatcggctt
     3421 gtcgtcgtgg aagcgtgcct cggggcgcag gttgaacacc acccagctgc ggtccttgct
     3481 gtactccacc gaggaggcga tcaggccgta gctggaggtc ggctcgtcgc ccgacggatc
     3541 gtaggcgcca gtgccggcca tcagcggggc attcagttcg gagacgccgt actgcaggaa
     3601 gttgccggtg ccggccgggc tggtgccctt tagggtatag gggttgaggg tatcgaaggt
     3661 gccggaggcc atcaggcgta ccgtgccgcc cttgggggcg tccggattga cccagtcgaa
     3721 atggctgaag ttggccgggt acttgagcgc gccgaactgg gcgtagccgt gccgttcggt
     3781 gacggcagca ctggtcggca gacaggccgc cagaccgagc agcagggcga ggagacgact
     3841 catcaggcga gggtccggtc cgtggcaatc ttgtaggttg agaaagctac agtaacagct
     3901 tgtatcggca ggaaaaagag tggacccggg aggtcgggcc gctggctcag cgccgcccga
     3961 ggtagagggt cagggtctgc ccgggcttga gggtgcggcc gagggagggg ttccactgtt
     4021 tcaggtgatg ggtgccgacc gcgaagcgct gggcgatgtc cgacagcgag tcgccgcggc
     4081 gcaccttgta gtaggttacc gactggcgcg tgtcgccccc gctcttggcc ttcgcggcgg
     4141 cacggggacg gtcttcgtgc agggtcagca cctgtccggc gtggatggat ttgcccttga
     4201 gcttgctcca gcgctgcagg tcggccaccg cgacgttgtg ctgcttggcc acgctccaca
     4261 gcgtatcgcc cttgcgcacc cggtagcggc gcagcgccgg tttggtcgtc gtttcggctc
     4321 tggcggcgac caccggcgcg ctgcgctcct cctgcacggc gttgccgggc agctgcggaa
     4381 tcagcagcgc ctggccggtg cgcacctgcc tgtcgggcag ctggttgagg tcgcgcaggg
     4441 tgctgctggc gacgccatgg cgcttggcga tgctgtccag gctgtcgccg cggcgcacct
     4501 ggtagcgctg ccactgcatc aggctgtccg gctgcagacg ggcgaggctc tcgctcagcc
     4561 cgtcggcctt ggccaccggc accagcaggt ggcgcgggcc atcgagggtg atgccctgct
     4621 tgaaggccgg gttgagctgg agcagctcgt ccttgtcgac atccgccatg gcggcgactc
     4681 gcgacaggtc gagctggtgg cggatggcga ctttttcgaa gtagggctga ttggcgatgg
     4741 gcgcgagcgc caccccatag ctggtcggtg cctggatgat ctgcgacacg gccagcagct
     4801 tgggcacgta gtcctgggtt tcccgcggca gcggcaggtt ccagtagtcg gtcggcaggt
     4861 tgcggctctg attgcgctcg atggcgcggc tgaccgtgcc ttcgccggca ttgtaggcgg
     4921 ccagggcgag cagccagtcg ccgttgaaca tgtcgtgcag gcgcttgagg tagttgatcg
     4981 cggcgttggt cgaggcggtg atgtcacgac ggccgtcgta ccagtcggtc tggcgcaggt
     5041 tgtagtggcg gccggtggag gggatgaatt gccagagacc ggcggcgtgg gccggcgagt
     5101 aggccatcgg gttgtaggag ctctcgatca tgggcagcag cgccagctcc agcggcatgt
     5161 cgttctcttc caggcgctcg acgatgtagt ggatataggg cccgctgcgt tcggcggtgc
     5221 gctggatcga cgccggatgg ctggcgaagc cgaagcgctg gcgctcgatg cgcgggttgg
     5281 tgccgatctg gtcctgcagc ttgtagccgg cgcggatgcg ctcccagatg tcgccgtgct
     5341 gctcttcctg gggcggtggc gtcttggtcc actcgatctg ccggctggct ctgacggtgt
     5401 tctcgggctc ggcgccgccg cggctctggc agccggccac gaccaccagc aagcccacca
     5461 gcaacagctg gagggcccgg gaagcggcgg tgcgaacgga actctggggc gttggcggtg
     5521 gcatcggctg gcgattttcc gggcaaaaag tcgggggatt ctaggaaccg tcccggaagt
     5581 ggtcaagttt tcaccagtta ctagaagtta tctttccagc tacgcaactc ggcaaaaact
     5641 gcactggggg agtccaggga atgcccggcc cgttgctcgc cttgctggcg caccccggct
     5701 tcgttgaccc gcaggaaggg gttggtcgcc cgctcgatgg ccagtgtcga gggcagcgtg
     5761 ggcaggccct gctcacgtag gctggcggct tcggcggcgc gttggtgcaa ggccggattg
     5821 tcgggttcca ccgccagggc gaagcgcaaa ttgctcaagg tgtactcgtg ggtgcagtac
     5881 acgcgggtct gcgccggcag ggcggccagg cgctgcagcg aggcgtgcat ctgcgcggcg
     5941 gtgccctcga acaggcggcc gcagcctgcg gagaacaggg tgtcgccgca gaacagcagc
     6001 ggctgcacgt cgtcgccacc gaacagggcg atgtgccctg cggtgtgtcc gggcacgtgc
     6061 agcacctgcc agtcgtggtc gaggaggcgg atgcgctggt tgtcctcgag ggcgacatcg
     6121 cggccgggaa tcgcttccag tgccgggccg agcacccgcg cgccggtgtg ctccttgagc
     6181 gcgaggatgc cgccggtatg gtcgtggtgg tggtgggtga ccaggatgtc ggtcaattgc
     6241 cagtccgcat gggtcgccag ccaggccagc accggcgcgc tgtcgccggg atcgaccacg
     6301 gcacagcgcc gctgcgcagc atcctgtaac agccagatat agttgtcgct gaaggcgggc
     6361 agggcggtga tctgtatcat gggcgggcga accactatgc tgagtcgatc cggcatctta
     6421 ggtcggcagg cgcgtctggc gccagacggg aggagtcatg aacgagcagc cctgcggcaa
     6481 cgccgccagc gactggctgg ccctgcagcg cgaagcccag gcctggttcg ccggaccgct
     6541 cggccgcctg ctgctggaca gtcagcaggc cctgctggag cagcagctgg gcagccactt
     6601 cggcagcttt ctgctgcact acggcttccc cggcggtgat ctgccgccgc aacggcagat
     6661 cacccactac gtccgtctcg gtccaccgct gccgggggtg gagatcgtct gcgaggagtg
     6721 ggcctggccg gtgctcgagc atgccgcgga tctggtcgtg ctgcagaatg ccctggattt
     6781 tgccgcctcg ccgcaccaat tgctgcgcga ggcggcgcgc tgcgtgcgtc cgggcgggca
     6841 tctgctgatc gtcggcattc atccctggag cctgtggggc gcgcggcatc tgctggccag
     6901 cgatgcactg gcgcaggcgc gctgcatctc gccggcccgt ctggccgact ggctgcacct
     6961 gctcggcttt gcgctggaga aacgccggtt cggatgctat tgtccgccgc tttcctcgca
     7021 gcgctggcag gcgcggctgg gtgccatcgc gccgtgggcc gagcgcctgc agctacccgg
     7081 cggcggcttc tacctgctgg cggcgcgcaa gctggtcgcc ggcctgcgcc cgctgccgca
     7141 gccccgacgt gccgccgttg cgctgctgcg tccggttccc gtggccaagg tcggccgtca
     7201 tccaggcaag aaagagtcat gactgacagc gtcgagatct ataccgatgg tgcctgcaag
     7261 ggcaatcccg gccccggtgg ctggggggcc ttgctgatct acaagggcgt cgagcgcgag
     7321 ctgtgggggg gggagtcgcc caccaccaac aaccgcatgg agctgaccgc ggccatccgt
     7381 gccctggaaa ccctcaaccg gcattgccgg gtgcagctga ccaccgattc cgaatatgtc
     7441 atgaagggca tcaaggagtg gctgccgaac tggaagaagc gcggctggaa gactgccgcg
     7501 cgccagccgg tgaagaacgc cgacctgtgg caggcgctgg acgagcaggt caatcgtcac
     7561 gaggtgcact ggcattgggt gcgcgggcac aatggccatc cgggcaacga gcgcgccgac
     7621 cagctggcca accggggcgt cggcgaggtc aacggctccc gccagtccgt gtgagcgctg
     7681 tcgcgtcctt cccttccgat ccactttcga acaggtcatc tcccatgcgc tatgtcgtcc
     7741 tcgacaccga gactaccggc cttgaggccc gtgaagggca ccgcatcatc gagatcggtt
     7801 gtgtcgaggt gatcggccgg cgtctgaccg ggcggcattt ccatgtctac atcaatcccg
     7861 aacgcgaggt cgacgagggg gcgctggcgg tccacggcat cagcaccgag ttcctcgccg
     7921 acaagccgcg ctttggcgag gtggtcgacg agttcttcga gttcatcaag ggcgccgagc
     7981 tgatcatcca caacgcggcg ttcgacatcg gcttcatcaa tcacgagttc gcccgcctca
     8041 aagaccgtcc gcagctgggc gaggtgctcg accattgcag cgtgctcgac accctgctga
     8101 tggcgcgcga gcggcatccc ggccagcgca acagtctcga cgccctgtgc aagcgctacg
     8161 gcgtcgacaa ctcggggcgc gagttgcacg gcgcactgct cgacgccgag attctcgccg
     8221 acgtctacct ggcgatgacc ggcggccaga ccagtctgtc gctggccggg cagaacggcg
     8281 aaggcgaggg cggcaacggg ccgcaggttg cggagattcg ccggctgccg gccgaccggc
     8341 cgctcactcg ggtgatccgc gccagcgaag cggaactgga gctgcacgcc gcgcgtctgg
     8401 ccgcggtggc caaggccgcg ggcgggccgg cgctctggca acagctggag caggccgccg
     8461 aggacgtggc gtcgaccctg cattgacggc aggtttcgcc cggcaggcgc agctactacc
     8521 ctgaaggcat cggcaggtgg agcctgtctg tttcgggggc tggagcatgt acaaggatct
     8581 gcgctttccg gtactcatcg tccaccgcga catcaaggcc gacagcgtgg ccggcgagcg
     8641 ggtgcgcggc atcatccacg aactggagca ggacggcttt accatcctgt cgacggccag
     8701 ttcccgcgaa gggcgcatcg ttgcttcctc gcagcacggg ctggcctgca tcctggtcgc
     8761 tgccgaaggc gcgggtgaca acccccgcct gctgcaggat gtggtcgagc tgatccgtgc
     8821 ggcacgggtg cgcgcgccgc agctgccgat cttcgccctc ggcgagcaga tcaccatcga
     8881 gaacgcgccg ccggaggcga tggccgagct caaccagctg cgcggcatcc tttacctgtt
     8941 cgaggacacc gtaccgtttc ttgctcgcca ggtggcgcgc gcggcgcaca actacctgga
     9001 cggcttgttg ccaccgttct tccgtgcgct ggtgcagcac accgccgaat ccaactactc
     9061 ctggcatacg ccgggtcacg gtggcggcgt ggcccaccgc aagagtccgg tggggcaggc
     9121 cttccaccag ttcttcggcg agaacaccct gcgctccgac ctgtcggtgt cggtgccgga
     9181 gctgggctcg ctgctcgatc acaccgggcc gctggccgag gccgagcgcc gcgcggcgcg
     9241 caatttcggt gccgaccaca ccttcttcgt gatcaacggc acctccacgg ccaacaagat
     9301 cgtctggcag gcgatggtcg gtcgcgacga cctggtgctg gtcgatcgca actgccacaa
     9361 gtcggtggtg catgcgctga tcatcaccgg cgccatcccg ctgtacctca atccggcgcg
     9421 caacgagctg ggcatcatcg ggccgattcc gctggccgag ttcagtcgcg agtcgatcgc
     9481 cgcgaagatc gccgccagcc cgctggcacg ggggcgcgag gcgaaggtca agctggcggt
     9541 ggtcaccaac tcgacctacg acggcctgtg ctacaacgcc gagctgatca agcgcgagtt
     9601 ggctgacagc gtcgaggtgc tgcatttcga cgaggcctgg tacgcctatg ccgccttcca
     9661 cgagttctat gccgggcgct acgccatggg cacctcgcgc gacgagggcg ggcgcgagcc
     9721 gctggtgttc agcacccact ccacccacaa gctgctggcc gccttcagcc aggcctcgat
     9781 gatccatgtg caggacggcg gccagcgcca gctggacgtg gcgctcttca acgaggcgtt
     9841 catgatgcac acctccacct cgccgcagta cggcatcctc gcctcgctgg atgtggcctc
     9901 ggcgatgatg gagggcgccg ccgggcgctc gctgatccag gaagccttcg acgaggcgct
     9961 ggccttccgc cgggccatgg ccaacctgcg gcgcaacctg ccggcagcgg actggtggtt
    10021 cgacatctgg cagccggaga ggggcgaggc ggcgctgcag gtgcagacca gtgactggct
    10081 gctggccccg caagcgccct ggcacggttt cggcgaggtg gccgaggatt acgtgctgct
    10141 cgatccggtc aaggtcaccc tggtcatgcc cggactgttc gccgacggcc gactggaccg
    10201 ggagggtatt cccgctgcgg tagttggcaa gttcctctgg gagcgtgggg tggtggtgga
    10261 gaagaccggg ctgtactcgc tgttggtgct gttctccctg agcatcacca agggcaagtg
    10321 gagcacgctg ctcaccgagt tgctggagtt taagcgcctc tacgacggca atgcgcccct
    10381 gggcgaggcg ctgccctcga ttgcccatgc caagcctgcg cgctatgccg gcatgggctt
    10441 gcgcgacctg tgccagcaac tgcacgcctg ctaccgtgac aacgcggtgc cgctggcctt
    10501 gcgacggatg tatacccgtc tgccggaggc ggtgctgaag ccggccgacg cctacgatca
    10561 gctggtgcgc ggtgcggtcg aggcggtgcc gctggatgcc ctggaggggc gcatcgcggc
    10621 ggtgatgctg gtgccctatc caccgggcat tccgctgatc atgcctggcg agcggttcac
    10681 cgaggacagc cggccgctca tcgactacct gaccttcacc ggccgcttcg aacggcagtt
    10741 tcccggcttt gctgccgatg tccatggctt gcagcgtcgc ggcggagcga agggcaaatg
    10801 gcacacggtg gattgcattc gcggctaagg ggcggtggtc gtgacctggg tcactatggt
    10861 cgattcgact tttaagggat gcttgatata gtgcagcaac tcaaagaagg gcagcacaat
    10921 cagctgcact cagtcgaaca accgccagga gccgaatctg tgtcgcgatg gatcgagacg
    10981 ggcatgccgg gcatgggcag ggaaggaagt tgccagcatg gtgatgaaca ggctttggat
    11041 gttgtcggac tgatggattc ctatcgcgtg ggaggcgcac aaggcgcatg gtaagtggag
    11101 ccacgtccct ggggctggtg cgcagcgagc tgttcgccac catcgagcag gcggaacaga
    11161 gtctcgagca gttcatcgcc gagcggcaca acgtcagcct gctgcaacag gcggtcgagt
    11221 acctgcagca aattcgcggc attctcaagc tgatcgagct ggttggtgcc gaactcctgg
    11281 cccaggaggt tctcctgctg gccaccgata ttccggctgg cgccggccag gagcgcgacg
    11341 gtcagttgtc cgcgctgggc aaggcgctgt tcgtcctgcg ccgctacctc gagggactgg
    11401 acaatcatcg ccaggaaatt cccgagctgc tgctgccgat gatcaacgag ctgcgccagt
    11461 ccaccgggca gccgagcctg ccggaaagct atttcttcag cgtccgcctg gatcagccgc
    11521 gccccgcacg gcgcaatgtg gagatccagc ccggcgtggc gaaccgcgac ggcacccgtc
    11581 tgcgccagat gtatcagctg ggcctgatga acgtcttgcg cgagcagaac ctgggcgcgg
    11641 cgctgaagct gatgtcgcgc tcgctgagcc gtctggatga tctgctgggg gcgcgcgagc
    11701 gttcgcggtt gtgctggatc ggcgctgcgg cgctggaggc catggccgag gcgcagctgc
    11761 gcccgaccaa gacgcgcaag cagttgttgg cgcgcatcga ccgcgatctt cggcagctgt
    11821 tgtccaatcc gcagttcgag gcgccgcgca gcctgctcaa ggagttgttg tacctggtgg
    11881 ccctggccgg cagccagggc gagcgcgcca gcgagatgcg ccgcgtgttc gggctggcct
    11941 cgctgccgtt taccgaccac ctgctggacg aggagtcccg gcgcctgagc ggcccaggtc
    12001 aggaggtcat gcgctcgctg tcgctggcca ttcgcgagga gctggtggcc gtcaaggata
    12061 tcctcgacct gatcgagcgc ggtgctgcgc aggtggagag caaggtcgag ctgcatgcgc
    12121 agatcggcaa gctggccaag acgctcggca tgatcggcct gagttcggcg gccaatgccc
    12181 tgcaggcgca ggtggcgacg gtcggttcct gggtcgagcg cagcgacaat ccggcggctg
    12241 ccgaactgct gcgtctggcc gatgccttgc tctatgtgga aagcatggta gcgaccctgg
    12301 agcagggcga tcagcgccag cgtcaggtga atgtggcgca ggcgaatggg cagggcgatg
    12361 gcgagtcgtt cgccacgcac cagctgttcg aggcgcatgt cgtggtcatg gacgaggctc
    12421 aggccggtct ggctctggcc aagcgtgcca ttaccgccta tctggaatcc aatggcgaca
    12481 agctcaacct ggccaacgtg ccaaccagcc tgcaggcggt gcgcggcggc ctgtggttcc
    12541 tcggtctggc gcgtgccgcc gagctggtcg gggcctgtgc cgactacatc cagaagcaca
    12601 tgatcgaaat cacccagatg ccgtccgagc agatgctgga aaccctggcc gatgcgctga
    12661 ccagtctgga gttcttcctc gaggggggcg ccaatctgcg gcctgaaggt cagcaggatg
    12721 tgctcgatct cgctgcggac agcgtgcgcg cactgggcat gcaggtcgct gcctgatggg
    12781 gggcgacctc tggcagccgg cggtcatcga ccccgcgcag gccggagggt gggccgtggc
    12841 gcattgccac cagcgcttcc tgggtgacag cagtggtgtg ctgtttcccc gcgagtggct
    12901 gaaggcccgg ttgccggctg cactggccga acacgggatc ggctatttcc aggggcagtc
    12961 ggtgttcgtc ctggagctgg cggagcctgt cgacggcctg ctgcccggcg gccagtggaa
    13021 gggactgcgt cagttcctgc tcgagggcga catttctacc taccgcatgc tgggctacgc
    13081 cgcgcagatc ggcacctggg cccgacagca tcgtttctgc ggcagttgcg gcggcccgtt
    13141 gcagccgttg tccggcgagc gggcgatgct ctgcccggcc tgcgacctgc atctgtatcc
    13201 ccggctgtcg ccgagcatca tcgtgttgat cagtcgcggc gaggagcttc tgcttgcgcg
    13261 ttcgccgcgc tttgccgccg gtctctacag caccctggcc ggcttcgtcg agccggggga
    13321 gtcgatcgag gaatgcgtgc gccgcgaggt gcgcgaggag gtggcgctgg aggtcgacaa
    13381 cctgcgctac atcgccagtc agaattggcc gtttccccat tcgctgatgt tgggcttcca
    13441 tgccgattac gccggcggcg agatcgtccc ccaggcggag gaaatcgagg atgcgcgctg
    13501 gttccgggtc gacgatctgc cgcagttgcc gccacacggc gccatttccc gctacctgat
    13561 tgagctgtac ctggcgcgtc gttcaggcgt ggccgagccg ctgctgcccg gctagcgccc
    13621 agccaggcct cgcttcatcc agacctcccc ccttgccacg gcgttggcgt acttgagcat
    13681 ggcttttcgc cgcatttctg ccgtcctgag gcataacaaa tcttgatctg ttagcgattg
    13741 tgcgcagctc gcgctggccg ctcgtggacg cgcgtccgca taatgccggc atctttcgtc
    13801 ggagaactcc tgtcatgcaa tacaacggac tggcctgggc gaccgccctg gcggcactga
    13861 tggtgctggc gatagccggg cgcatcctgg gcaatcgcag ctggtttctc ggctggttgc
    13921 gcggtacctg cggactggcc ttgctggcgc tggccctgct ggccggcgtg gtggcttacg
    13981 atctgcacag ctatgcgcct ttgccgcggc aggggcaggc gttgctgacc ctgagctttc
    14041 aggccgaggg cgcgcagcgc tatcgtgcca cggtgcgtga aggcgggcgc gaacgcagcg
    14101 tgatcctgtc cggtgatctc tggcagctgg atgcacgcgt gttcgactgg aaggggctgg
    14161 cgagcctgat cggcctgcag cccggctatc gtctggaact gctggccggg cgttacctgt
    14221 ccgcggagca gcaggccgct gccgagccga gtcgcatcgc cttggcgtcc agcccttatg
    14281 gtatcgacgt gtggcgctgg ctgcgcgagg gccggcatga cctgtttctg ttcgatgccc
    14341 gcggggcgcg ggtgaccttc ctgccgatgg ccgacggtgc ggtctattcg gtcagcctgg
    14401 gttcggccgg cctggtggcc gagccgcaga ctcaggctgc gagggctgcg ctgtcccggt
    14461 agaggttgcg cgccgcggct gttttttcga atcccaaaaa gaaaaacccc ggtcaaagtg
    14521 accggggttt ttctttttag cagtctcggc cccgaggggc cgagtgagcc ggttccttag
    14581 gagaactggt tcatggtgtt gtctttaccg cttgccttca gagcagcttc gccagcgaag
    14641 tactccttgt ggttgtcgcc gatgtcggag ccagccatgt tctggtgctt gacgcaggcg
    14701 atgccctgac gcagttcctg acgctgaacg cccttcacgt aggccagcat gccttcttcg
    14761 gcgaagtagc ccttggccag gttgtcggtg gacagcgcgg cggtgtggta ggtcggcagg
    14821 gtgatcaggt ggtggaagat gccggcatga gcggaaccgt cgcgctggaa ggtgcggatc
    14881 ttctcgtcag cgatctgagc cagttcggtt tcgtcgtact cgacgctcat cagcttggcg
    14941 cggtcgtagg cggaaacgtc cttgccttcg gcggccatgg cgtcgaacac ctgctgacgg
    15001 aagttcaggg tccagttgaa cgacgggctg ttgttgtaca ccagcttggc gttcgggatc
    15061 accttgcgaa cttcgtccat catcgcagcg atctggccaa cgtgcggctt ctcggtttcg
    15121 atccacagca ggtcggcgcc gttctgcagc gaggtgatgc tgtccaggac gcagcgagct
    15181 tcgccagtac ccttgcggaa ctggaacagg ttgctcggca gacgcttcgg acgcagcagc
    15241 ttgccttcac gcttgatcac gacgtcgccg ttgcccagtt cggcttcggt gatttcttcg
    15301 caatccagga aggagttgta ctggtcgccc aggtcgcccg gctgcttggt tacggcgatc
    15361 tgcttggtca ggccggcacc cagggagtcg gtacgagcaa cgatcacgcc gttgtcgatg
    15421 cccagctcga ggaaggcgta acgaacagca gcaaccttgg ccaggaagtc ggcgtgcgga
    15481 acggtcactt tgccgtcttg gtggccgcac tgcttctcgt cggaaacctg gttttcgatc
    15541 tggatgcagc aagcgcctgc ttcgatcatg cgcttggcca gcaggtaggt ggcttccggg
    15601 ttaccgaagc cagcgtcgat gtcggcgatg atcggtacga tgtgggtttc gtagttgtcg
    15661 atctgcgact ggatttcggc tgccttggcc tggtcgccag cttcacgagc ggcgtccaga
    15721 gcggtgaaca gcaggtccag ctcgcgggag tcggcctggc gcaggaaggt gtacagctct
    15781 tcgatcaggt tggaaacggc agtcttctcg tgcatcgact ggtccggcag cgggccgaac
    15841 tcagaacgca gggcagcaac catccagccg gacaggtaca ggtagcgctt gttggtggtc
    15901 ttcaggtgct tcttgatgga gatcagcttc tgctgaccga tgaagccgtg ccagcaaccc
    15961 agcgactggg tgtagacaga ggagtcggca tcatactcgg ccatgtcctt gcgcatgatg
    16021 tcggcggtgt actgggcgat ttccaggccg gtcttgaagc gattctgagc gcgcatgcga
    16081 gcgacggact cagggttaat cgcgctccag ctgctgccaa attgttcttt cagggcggca
    16141 atggccttga tgtcgttttg ataagcggac atggtcaatc cttcaaaaag agtgtttcgt
    16201 tgagcaccga cattcccatt caacacgtgg gtgttgacgg tagtgttgca agagctcacc
    16261 gcagagcgtc gaggtggaga ctggtacggc aggaggccgt tgagagggga tgtagtgacg
    16321 accggtgggg tgactacaac agccggatag gttgtgttcg tcatgccggt tgaaaccttg
    16381 cttccccgtc cctcgggaca acttcgttcc agtcgcaacc ttgtcgcacc gccgtgtggg
    16441 tggtactgac acgaaccgat tccagggatg gtctggactg cgatccggaa gccatttcgg
    16501 gcctctggtc agcgggagcg aggtcatgat gctaggagcg cggcggggcg tcaatggctt
    16561 ttgtagtttt tttttggagc tactacaaaa gttctagatc agtcgagaag ctcgaccttg
    16621 aggcgcagcg cctggttttg ccgggcgttg cgggcgtcct gcgagaggat ggcggagcgg
    16681 ttgccggaga cgctttcgct gaccccaccc agctcgatcc attcgccgag gcggccgttg
    16741 atgcgggtat cggtgtcctg ggtgtcgatc acgccgggcc ggctgcccag gcgatcctgc
    16801 tgactgcgga tgctgagctg gaccatctcg ccgtgcacgc tggccaccac ctcgaagccc
    16861 cggttgagat cgcggtattc ggtgtattcg cgggggtagc cgtaggcgtc gcgctggata
    16921 cgggtcagcg gcacgctctg gccggtctgg atcagcgccg gatagccttc ggtggcctgg
    16981 acctgctgca gcgagccgct gctgccgcgg ctgctgcggt tgatgatgcg cagctcgtcg
    17041 cggccgtgct gttcgccacg cccgccgctg atctcgacgt tgccggcatc gatactaccg
    17101 tcgacccggt agccctggcg gttgctgctg ctgtcctgct ggctgtccac gctgatcagc
    17161 aggcggcgcg ggcgggtgtc cagttgctgc agcagttcct gcagttcggc gagcttggct
    17221 ggctcggcat tgacgatcag ctggttgccg taggcgctga cgcggccctc gtggcccagt
    17281 acggattgca ccagcggcag cagttcttcg gcggtgcggt actgcagcgc caggatttcg
    17341 gtacgcggcg cggcctgcag ggcgagactg cagaccagcc cgaggagcgc cagccatggc
    17401 cgcaggttca tagcagcagg ctccgcaggt tgggatcgcc gaggctggtg ttccaggcct
    17461 ggtcgaattg gcgccgcagc tggcgcacac gcgccgcggc ctggtagtgg acctggctgg
    17521 cgggcagcag cggtgccgta cgcagcagca ggccgcagtc gtcgaccagc aggtaggcga
    17581 ggtcttcggc ggggtagtcg gggtgcagct tgcggatctg gcagttgctc gacagctggc
    17641 gggcgagtct gagcaggtga tggccgttgc gcacggccag gctgctgtcg cgcagcagga
    17701 tgcgcaggcg gttgcgcgga tggccacgca ggaagcgact gcaggcctcg gcgatggcgg
    17761 gatgatcgta cagccagggc tcgagatccg gcgtgtacag gcacaggtcg cggcgtgcct
    17821 ggccgagcag ggcgagggca tggctgcgca gttgctcgcg tgccgtgcag gtttccacgg
    17881 gcgcatgtgc gccgaggagg aagggcgccg gctctaggtg gccggcgtct tcgcgcgtcg
    17941 ccgctggcgg gttgtggatg gcaaaacgcc ccggagagcg gaattcgaca gcgggcagtt
    18001 cgctggcgtc atcgttggct tgctccgggg cgttgctcat gggggcctct caggcgctgt
    18061 cgcgcaccat ttcgacgtgg gggatgccgg cctcgaggaa ttcctcgctg gccacccgga
    18121 agcctagctg ttcgtagaaa cgaatggcat gggcctgggc ggtcagtttc tgttgcttga
    18181 ggtcgcggcg ctcggcctcg gcgatggtcg cctgcatcag tgcctcgccg atgtgcaggc
    18241 cgcgccagtc cttgagtacc gacacccggc cgatctggcc gtccggcagc aggcggctgg
    18301 cgccgacggc aaagtcgccc tcgaaggcca ggaagtgcac ggcgtcagtg tcgtcggcat
    18361 cccattccag ctccggcgac acgccctgtt cgacaatgaa caccgcctcg cggatgcgcc
    18421 gcaggtcggc gttgtcggcg tgccagtcgg caacccgtac gctgatctta ttcatcggcg
    18481 gcaaactcca ggctgccctg cttgaccagt tcgtgaatca gcgtcagcgc ctcgtcgctt
    18541 tccagccagg gctcgaggtt ttccaggtgc agggcgtcgg cactgcacac cagcttgagc
    18601 aggtcgcgca gcgaggcgtc gacgcgccgg ctgttgccgc tggcaaacag caccaggtag
    18661 tcgtcgactt cgctccaggc caggcgggcg ctggggttgc gcaccagcac ggcgccgtcg
    18721 ccgagggcgg tgaaaaactc cgcttcgtcg atctcttcgc cggcgaccat ttccgggtaa
    18781 cgcggttcgg tcatgaactg gccgaaccag gtcagcagca gtttctcgtc gctcatgtgg
    18841 cggtcgatca gactgcgcag gcgctcgagg gcgtcgcgct ggatctggtt cgggtcgccc
    18901 gcgggctggc agccggcgtc gctgtagcgt tcctcgtcgg ggaggaactg gccgaggaag
    18961 tcggtgaagt gggtcagtac ttcgctggcg ctgggcgccc ggaagccgac cgagaaggtc
    19021 atgcactcgt cctcggcaat gccgtagtgg gccaggcgcg gcggcaggta gagcatgtcg
    19081 ccgggctcca gcacccactc gtcggtctgc tcgaagtcgg cgaggatgcg caggtccggg
    19141 tggtcgagca gcgggctgtc gctgtcgcac atctggccca gcttccagcg gcgctggccg
    19201 tgggcctgca gcaggaacac gtcgtagttg tcgaagtgcg ggccgacgcc gccgcccggg
    19261 gcggcatagc tgatcatcag gtcgtcgatg cgccagctgg gcaggaacag gaactgctcc
    19321 atcaattcgc gcatgtcggg cacgaactgg tcgaccgcct gcaccagcag ggtccagtcg
    19381 cgctccggca ggtgctggta ggcgtcttcg gcgaacgggc cgcggcgcag ctcccagggg
    19441 ctgttgccgt gctcgaggac gatgcgcgac tcgacttcct cctccagcga caggccagcc
    19501 agctcgtccg gggagatcgg gctctggtag ccggggatgg cctgacggat cagcagcggc
    19561 ttctgctgcc agtagtcgcg gagaaattcg cggggcgtca ggccgcccag cagttgcagc
    19621 ggggtatcgg gtgccatatc caactcttca ataatgaaaa cgcccgacct cgagaccggc
    19681 ttggtggctg ccagtctgga cggtcgggcg aagggccgtt caggctggga tcagatgcgc
    19741 ttggcctggg ccaccgcgtt gccgatgtag ctggcggggg tcatggtgcg cagttcgctc
    19801 ttggcctgct ccgggatcgc cagggtgtcg atgaactcgc gcagcgcctc gggggtgatg
    19861 cccttgccgc gagtcagctc cttgagcttc tcgtaggggt tctcgatgcc gtagcgacgc
    19921 atcacggtct ggatcggctc ggccagcact tcccagcagg cgtcgaggtc ggcggccagg
    19981 cgctgggcgt tgagctccag cttgccgatg cccttgaggc tggcctcgta ggcgatcagg
    20041 ctgtgggcca ggccgacacc gaggttgcgc agtacggtgg agtcggtcag gtcgcgctgc
    20101 cagcgggaca ccggcagctt gctggccagg tgctggagca gggcgttggc gatgccgagg
    20161 ttgccctcgg agttctcgaa gtcgatcggg ttgaccttgt gcggcatggt cgaggagccg
    20221 atctcgccgg ccacggtctt ctgcttgaag tagccgaggg agatgtagcc ccagacgtcg
    20281 cgatcgaagt cgatcaggat ggtgttgaag cgcgcgatgg cgtcgaacag ctcggcgatg
    20341 tagtcgtgcg gctcgatctg ggtggtgtag gggttgaacg ccaggcccag gtcgccttcg
    20401 atgaattcgc gggcgttggc ttcccagtcg atctccgggt aggccgacag gtgggcgttg
    20461 tagttgccca cggcgccgtt gatcttgccc agcagcggta cggcggcgac ctgggcgatc
    20521 tgccgctcga ggcggtagac cacgttggcc agttccttgc ccatggtggt cggcgaggcc
    20581 ggctggccgt gggtacgcga cagcatcggc acgtcggcga actgcacggc gagggtgcgg
    20641 atcgactcgg ccacctggcg catcagcggc agcagcacgt cgtcgcggcc ttcgcgcagc
    20701 atcagggcgt gggacaggtt gttgatgtcc tcgctggtgc aggcgaagtg gatgaactcg
    20761 ctgaccttgg ccagttccgg cagcttggcg gcctgctcct tgagcaggta ctccacggcc
    20821 ttgacgtcgt ggttggtggt gcgctcgatg tccttgatgc gctgggcatg ctcgagctgg
    20881 aagttttccg ccagctcgtt gagcagggcg ttggcttcgg cggagaacgg cgccacttcc
    20941 gggatgccgg cgtgggcggc caggcgctgc agccagcgca cttccacctg cacgcggcaa
    21001 cggatcaggc cgaattcgct gaagatcgga cgcagggcgt tggttttgct gccgtagcgg
    21061 ccatcaacgg gggataccgc agtgagcgag gagagctgca tggggacgtt ctcggacagt
    21121 cgggctactg gaaaggggcg gaattataca cggaaagacg gcggccggtc cgtgctcggg
    21181 ctgagcggcg gcgcagcgga ggatcaggct ggcggccagg agcggtgcca cgccccggcc
    21241 tgggctgccg ggccgggggc ttggggctca gtcgcgcagc agcggataca gctggtcgag
    21301 cagcttgcgc cggctgaaca gcagctgcca gcggcgaccg ccgagctggt gccagagcat
    21361 ggcgctacgg atgccggcga gcagcagggc gcggatcttt tcggcgttgc ggctgttctg
    21421 caggtgctgc atctcgccgt gcacctggat gcgctggcgg aaggtgctga gggtgtcctg
    21481 gtacagggca gcgcaggcgg agatcacgtt gtcgtgggtg atgccgaaca tctggctctg
    21541 gctgtcgatc tgctgcaggc ggttgccgat cacctgcagc atgtcgccgc gcttgtccag
    21601 ctggcgctgc agggtgagca gggacagggc atagcgcagc gattcgcgtg gcagggtgcc
    21661 cgggttgcgc tcgagggccc cggccagggc gcgcaggccg tcgcgcaggt tgaggtggtc
    21721 gccgccgtag acttccaggg tgtccttggg gttgcgcacc agcaggctgc cgagcatgca
    21781 ggccagcggg ccttcctggg cctggccggt cttggccagg cggtcgacca gcgcggcggc
    21841 ttcgaacacg ccggccaggg cgatcaggcg ttcctgttcg cgggtcatgg gcgctggatc
    21901 tccgcatccc agggggaggc ggtctcgatc acgccgccgc ccaggcagat ttcgccgtcg
    21961 tagaacacca ccgactggcc tggggtcacg gcgcgctgcg gctggtcgaa gatcgcgtgg
    22021 tagccgttgg cggtccgttc cagggtgcag gcctggtcct gctggcggta gcgcaccttg
    22081 gcggtgaggc ggcgcggctg gtcgaggtcg acggggttga cccagaagat ctgcgaggct
    22141 tccaggctgc gcgagaacag ccagggatgc tcgttgccct ggccgaccac cagtacgttg
    22201 cgggtcatgt tcttggccag cacgtaccag gcgtcgtcgc cggcatcctt catgccgccg
    22261 atgccgaggc cctggcgctg gccaatggtg tggtacatca ggccgtggtg gcggccgatc
    22321 acctggccgt cggtggtctc gatgtcgccg ggctgggccg gcaggtactg cttgaggaag
    22381 tcgctgaagc gccgctcgcc gatgaagcag atgccggtgg agtccttctt cttcgcggtg
    22441 gccaggccgt actgttcggc gatggcgcgt accgccggct tctccagctc gccgaccggg
    22501 aacagggttc tgccgatctg ctcgccgccg acggcgtgca ggaagtagct ctgatccttg
    22561 ttcgggtcga ggcccttgag cagctcgctg cggcagtcga cgtcgcggcg gcgcacgtag
    22621 tggccggtgg cgatcaggtc ggcaccgagc atcagggcgt agtcgaggaa cgccttgaac
    22681 ttgatttccc ggttgcacag gatgtccggg ttgggcgtgc ggccggcctt gtactcggcg
    22741 aggaagtgct cgaacacgtt gtcccagtac tcggcagcga agttggcggt atgcagctgg
    22801 atgcccagct tgtcgcagac ggcctgggcg tcggccagat cttccctggc ggtgcagtat
    22861 tcggtgccgt cgtcctcttc ccagttcttc atgaacaggc cttcgacctg atagccctgc
    22921 tgctgcagga gcagggccga gacggaagag tccacgccgc cggacatgcc gacgatcacg
    22981 cgggtacggg ctgggtcacg catagtcatc cggagaaacc gttgctgggt ggcgaaacgc
    23041 caattctatc aggcaggggc atgggcggcg atcaagctgg cgtgcggatc agttccagcg
    23101 gatagctgcc gccagccaga tagtcgtcga tgcagcgggg taccagatcg ctgcgccact
    23161 gctcgtggcg ggcttccagc tcttcgcggg tcagccagcg cgggccgatg atgccgtggt
    23221 cgagcggcag ttccgggcgg tggcgcagcg ggcgggccgc gaagcacacg cgctggtagg
    23281 tgacgccgtt ggccggggcg gtgtacaggt agatgccggt cacgccggtc aattcgacct
    23341 cccagccggt ctcctcgagg gtttcgcgca gggcggcctg gagcaggctc tcgttggcct
    23401 ccaggtggcc ggccggctgg ttcagcacca tgcgcccttc ggcgtgctcc tccaccagga
    23461 gaaagcggcc ctggtcttcg accacggtgg ccacggtaac gtgcggaata aagcgtttca
    23521 tgcgggactc cggtttcagt cttgcggata caagaacccc ggcgctaggc cggggttctt
    23581 gtggtgctaa tggcgactcg cgtcgccagt caggcagtcg tcgcgcttat gccagtgcgg
    23641 ccagggccgc attgaaggtg gcgctcgggc gcatggcctg gctggtcagc tcggtatcgg
    23701 catggtagta accgccgatg tccaccggct tgccctgggc ggcgttcagc tcggcaacga
    23761 tcttttcctc gttgtcggcc agcgccttgg ccaggttggt gaactgcgcc tgcagctcct
    23821 tgtcctcggt ctgcgcggcc agggcctggg cccagtacag tgccagatag aagtggctgc
    23881 cgcggttgtc gagctggccg accttgcgcg acggcgactt gtcgctgtcg aggaactggc
    23941 cgttggcctg gtccagggtc ttggccagta ccttggcctt ggcgttggcg caggtagtgc
    24001 cgacatgctc gagggaggcg gcgagagcga ggaactcgcc gagcgaatcc cagcgcaggt
    24061 ggccttcttc caggaactgc tggacgtgct tgggtgccga accgccggcg ccggtttcga
    24121 acaggccgcc gccactcatc agcggaacga tcgacagcat cttggcgctg gtgcccagtt
    24181 ccatgatcgg gaacaggtcg gtcaggtagt cgcgcaggac gttgccggtc acagagatgg
    24241 tgtccaggcc ggcgcggatg cggtcgatgg agaactgggt ggcttcctcc ggcgacatga
    24301 tgcggatgtc gaggccgctg gtgtcgtggt ccttcaggta ctgctggacc ttctcgatga
    24361 ccagggcgtc gtgggcgcga gccgggtcca gccagaatac ggccggggtg ttgctggcgc
    24421 gggcgcggtt gacggccagc ttgacccaat cctggatcgg tgcgtccttg gcctggcaca
    24481 tgcgccagat gtcgccggtc tcgacggcct gctcgagcag gacctggccc ttgtcgtcgg
    24541 tcacgcggac cacgccgttg gacgggatct ggaaggtctt gtcgtgcgag ccgtactctt
    24601 cggcttgctg ggccatcagg ccgacgttgg gcacgctgcc catggtgacc gggtcgaagg
    24661 caccgtgctt cttgcagtcg tcgatgacga cctgatagac gccggcgtag cagcggtcag
    24721 ggatcagggc cttggtgtcg tgcagcttgc cgtcggcgcc ccacatcttg ccggagtcgc
    24781 ggatcatggc cggcatcgag gcgtcgacga tgacgtcgct cggcacgtgc aggttggtga
    24841 tgcccttgtc ggagttcacc atggccagcg gcggacgctg ggcatagacg gcctggatgt
    24901 cggcctcgat ctcggcctgc ttctcggcgg gcagggtctt gatgcgcgcg tacaggtcgc
    24961 cgatgccgtt gttgaggttg aagccgattt ccttgaggac atcggcgtgc ttctcgaggg
    25021 cgtccttgta gtactcggcg acgatgtagc cgaacatgat cgggtcggag accttcatca
    25081 tggtggcttt caggtgggcc gacagcagga cgccttgctt cttggcatct tcgatctcgg
    25141 cggccacgaa gctgcgcagg gccttctggc tcatgatcga agcgtcgatg atttcaccgg
    25201 ccagaacggc ggttttttcc ttgaggacct tgctgctgcc gtctgcggcg atcagttcga
    25261 tcttgacgct gccggcggcc tcgatcaggg cgcctttttc ggtgccgtag aagtcgccgc
    25321 tgttcatgtg ggcgacgtgg gtcttggagt ctgcactcca ggcacccatc ttgtgggggt
    25381 gcttgcgggc gtagttcttg accgagagcg gggcgcgacg gtcggagttg ccttcgcgca
    25441 ggaccgggtt cacggcgctg cccttgatct tgtcgtagcg gccgcggacg tccttttcgg
    25501 tgtcgctggc gggggcttcg gggtagtcag gcagcttgta gccctgctgc tgcagttcct
    25561 tgatggcggc cttgagctgc ggcaccgagg cgctgatgtt cggcagcttg atgatgttgg
    25621 cttctggcgt ggtggccagt ttgcccagct cggcgaggtg gtcgccgatt ttctggtctt
    25681 cgttcaggta ttcgggaaag cttgcgagga tgcgacctgc gagggagatg tcccgtgttt
    25741 caacggagat gcccgaggag gcggtgaagg cttggatgat cggaagcagg gagtaggttg
    25801 ccagggcagg tgcttcgtcg gtaaaggtgt agatgatctt tgagcgagtg gacatctttt
    25861 aatctaaccc tcagttttgc tgtggttaca gaaagtcttg atgcgcgcaa gaggatgctt
    25921 cttgaggcga tttcgggcgc atcggctttt ggccatgggc ggctattatt aagtggcgta
    25981 gaccaacacg cacactggac tataggcgca gaacccccac tccagtgcag ctcagtcccc
    26041 tgtgccaccg cagtcgcggc gctaggatat cacgatatgc cgctggcttt catgagggct
    26101 gatcggcgtc tagggggctt cctgccggct tttcatgcac tggcgagggc agcagatcgt
    26161 ttgcgtgcat ggggccgcca gacctgtccg agaggggcgt tccggccctt gatattgatg
    26221 aaaaaaaggc cggtcctggt tggtggtcgg ccttttgtcg ccgtacgccg gcgtgcctcg
    26281 gctcagtacc cgctgctgcg cggtggtgtg gtgctcacag gcagcggctg gatgttgacg
    26341 gcatgcagcc ccttggggcc ttgcaggatc tcgaagttga ccgattggcc ggccttgagg
    26401 gtcttgtagc catcgctctg gatggccgag tagtgcgcga acagttcttc atccctgcct
    26461 tcggccagga tgaagccata gcccttggcg ttgttgaacc acttgacctt gccgctcagc
    26521 atgctgactt ccctctgcaa aggactccat cgctggagta tcatccgtca tgaattacgc
    26581 ggatggatgg ggcgctccgc gcttcaatcc ccttcaccct ttatgggtat gcctttctgt
    26641 ttgtaacact gttttgccga gagtcaaggc gaggcggcat tcgccggccg aatcgcgcaa
    26701 actggttaca gccattccat tcaccagcca gtggtgacca cgccagcatg catgcacgca
    26761 gccagattcg actaacattc aatcaggact cgccagatcc gtctggaggc gaggattcgg
    26821 gtgtcgcaat ccaggaggcc aagccggcac tcaaggcgcc gccgatgtac aaggtcctcc
    26881 tgctcaatga cgattacact ccgatggact tcgtggtcga agtactggag agcttcttcg
    26941 ggatgaaccg ggaaacggca acccggatca tgctggcggt gcataccgaa gggcgggcag
    27001 tgtgcggggt ctatacccgg gacattgccg agaccaaggc gatgcaggtc aatcagtacg
    27061 ctcgcgagtg ccagcatccg ctgttgtgcg agatcgagaa agacgagtga catggcctat
    27121 ggcttgggct tttgaggaat gaggtgaagt tatgttgaac cgcgaacttg aagtcaccct
    27181 caacctcgcc ttcaaggagg ctcggaccaa gcgtcatgag ttcatgaccg tcgagcacct
    27241 cttgctggcc ctgctggata acggtgccgc cgcgaccgtg ctgcgtgcct gcggcgccaa
    27301 catggacaag ctgcggcatg acctgcgtga gttcatcgac tcgaccacgc cgctgatccc
    27361 gctgagcgag gatgaacgcg aaacccagcc gaccctgggc ttccagcgcg tgttgcagcg
    27421 cgcggtattc catgtgcaga gctccggcaa gcgcgaggtg accggggcga atgtgctggt
    27481 ggcgatcttc agcgagcagg agagccaggc ggtattcctg ctcaagcagc agagtgtcgc
    27541 gcgcatcgat gtggtcaact acatcgccca tggcatttcc aaggtgccgg gtgaaagcga
    27601 gccgcacgaa caggagcagg aatcccagga cgaggagggt ggagagagtc cggtgtccgg
    27661 caatccgctg gatgcctacg ccagcaatct caacgagcag gcgcggcagg ggcgtatcga
    27721 tccgctggtc ggccgcgagc ctgaggtcga gcgcgttgcg cagattctcg cgcgccgccg
    27781 caagaacaat ccgctgctgg tcggtgaggc cggtgtcggc aagacggcga ttgccgaggg
    27841 cttggccaag cgcatcgtcg atggccaggt ccccgacctg ctggccaata gcgtggtcta
    27901 ctcgctcgac ctgggtgcat tgctggccgg gaccaagtat cgcggcgact tcgagaagcg
    27961 cttcaaggcg ctgctcaacg aattgcgccg gcggccgcat gcgatcctgt tcatcgacga
    28021 gatccacacc atcattggcg ccggtgcggc atccggtggg gtgatggatg cttcgaacct
    28081 gctcaagccg ctgctgtcct ctggcgagat gcgctgcatc ggttcgacta ctttccagga
    28141 attccgcggc atcttcgaga aggatcgggc cttggctcgg cgcttccaga aggtcgacgt
    28201 gctcgagccc tcggtggagg ataccgtcgg catcctccgt gggctgaaga gccgcttcga
    28261 gcagcaccac aagatcagct acaccgacga ggcgctgcgt gccgccgctg agctcgcagc
    28321 gcgctatatc aacgaccggc acatgccgga caaggccatc gatgtgatcg atgaggctgg
    28381 cgcctaccag cggctgcagc cggagagccg gcgtgccgcc tgcgtcgacg tgccgcagat
    28441 cgaggatgtg gtggcaaaga ttgcccgcat tccgcccaag catgtcacca cttcggacaa
    28501 ggagctgctg cgcaatctgg agcgcgatct caagctgacg gtgttcggcc aggatgcggc
    28561 gatcgatgcg ctgtccaccg ccatcaagct gtcgcgggcc ggcctcaagg cgccggacaa
    28621 gccggtcggc tcgttcctct ttgccggccc gaccggcgtc ggcaagaccg aggtggcgcg
    28681 ccagctggcc aaggcgctgg gggtggagct ggtgcgtttc gacatgtccg agtacatgga
    28741 gcggcacacc gtgtcgcgcc tgatcggcgc gccaccgggg tacgtcggct tcgatcaggg
    28801 tggcctgctg accgaagcga tcaccaagac accacactgc gtgctgctgc tggacgagat
    28861 cgagaaggcg catccagagg tgttcaacct gcttttgcag gtcatggatc atggcacgct
    28921 gaccgacaat aacgggcgca aggccgattt ccgcagtgtg attctgatca tgaccaccaa
    28981 cgcgggggcg gaaacggcgg cacgcgcgtc gatcggcttc accctgcagg accactccag
    29041 tgacgccatg gaagtgatca ggaaaagctt caccccggaa ttccgcaatc gcctggatac
    29101 catcattcag ttcggccggc ttgaccatac gatcatcaag agcgtggtcg acaagttcct
    29161 caccgagttg caggcacagc tggaggacaa gcgggtgctg ctggatgtca gcgaggcggc
    29221 gcgtggctgg ctggccgagc gcggctacga tgcgcagatg ggcgcgcggc cgatggcgcg
    29281 gctgatccag gacaagatca agcgaccgct ggccgagcag atcctgttcg gcgaattggc
    29341 cgagcagggt ggcaccgtgc acatcgatgt gcgggagggt gagctggtgt tcgattacga
    29401 gacggtggcc ttgccagcct gacagtgcgg aaggctgtaa aaacgacaac gcccggcaat
    29461 gccgggcgtt gtcgtttcat gaccttgcgt cgatcaacga gcgcgatagg tgatgcgacc
    29521 cttgctcagg tcgtagggcg tcagttccac gcggaccttg tcgccggtca ggatacggat
    29581 gtagttcttg cgcatcttgc cggagatgtg cgcggtgacg acgtgcccgt tttccaactc
    29641 cacgcggaac atggtgttgg gtagggtgtc gacgacagtg ccttccattt cgaagctgtc
    29701 ttctttcgac atgcagtaaa gccctcaatt acctaaggat ggcccgattg acgtgctcgg
    29761 gcaaaatcgc ctggcattgt gcccgaaaaa cccctccgca gccaagtagc ctcagtcgag
    29821 ggtcacccag tgctggttca cgtacagctc cagcggacga tactcgctct tgtaattcat
    29881 cttgcggcag ttgcggatcc agtagccgag atagacggcc ggaagcgctc ggcgggatgc
    29941 ttcggctacc tgccagagaa tcgccacgcg cccgagactg cgcctttcct cgagtggatc
    30001 gaagaaggta tagaccgcag acaggccgtt gggtaggacg tcggtcacgg cgacggccag
    30061 cagtcgtccc tgcaggcgaa actcgaagaa cacgcagaac ggcaggtcac tcaccaaaaa
    30121 ggtggtgaac tgttcgcggc tgggtggata catgtcgccg tccgcgtggc gctgctcgat
    30181 atagcgggcg tagaggtcgt aatactcctc gcgaaattcc ggacgaacgg cgtgcacgct
    30241 gaggtcccga ttgcgcttga ggatgcgttt ctgttgctgg ctggcctgaa agctggcggt
    30301 gggaatgcgc gccggaaggc aggcctggca atgctggcaa tggggccggt acagatgttc
    30361 gccgctgcgc cggaagccca gttccgacag gccggcatac atgccggcat cgaacgggtg
    30421 gcgcggatcg agaaacaggg tggtcgcctg ctgtccggag aggtagctgc agtcgtgcgg
    30481 ctgggtcgca tagaacttca gatgggccag ttcggtcatg acgtaacccc cggtagtgca
    30541 ggtcgagcgg gatcgccttt accagtgtaa gtcacgccgg ggtgctcgcc caggatgtcg
    30601 ggccggactg gtcaaggtag cgctcgaggt agccggcaaa atcggcacga ggtatctcgc
    30661 gggctccgaa gctggacaga tgctgggtgt gcatctggca gtcgatcagc acgtagccgc
    30721 attcgcgcag ctgttcgacc agggtgacga agccaatctt cgaggcgttg tccgtgcggc
    30781 tgaacatcga ctcgccgaaa aacaggcgcc ccaaggccag tccgtagaga ccgccgacca
    30841 gttggtcttc ctgccagacc tcgaccgagt gggcgatgcc gcggcgatgc aattccagat
    30901 aggcagcctg catcgctcgg gtgatccagg tgccgtcagc atagctgcgt ggcgcggcac
    30961 aggcgcgaat caccgcggga aagtcacgat caaaactgac ctcgaagcgt ccctggcgca
    31021 tcagcttgcg caggctgtgc gagacgtgca gctctgcggg gaacaggacc atgcgtgggt
    31081 cgggcgacca ccagaggatg ggctgccctt cctggaacca gggaaagcag ccgtgccggt
    31141 aggcgctgat cagtcgctcg gggctaaggt cgccgcctgc ggcgagcagg ccgtccggct
    31201 cgcgcagtgc ctgctccagc ggggggaact tcagggagtc gcgtgccagc cagttgagca
    31261 tgctggggga ttactgctcg aggaagcgtt cggcgtcgag tgccgccatg cagccggcgc
    31321 cggcggaggt gatggcttgg cggtagacgt ggtcggccac gtcaccggcg gcaaataccc
    31381 cctcgatgct ggtggcagtg gcattgcctt cgccgccacc ctttaccttc aggtagccgt
    31441 cgtgcatcgc cagctggtcg gtgaacagct cggtgttggg cttgtggccg atggcgatga
    31501 aaacgccggt cagcggcagc tcctgggtgg cgccgttgtt cacgtccttg aggcgcacgc
    31561 cggtcacgcc gctggcgtcg ccgagcactt cgtccaaggc ctggttccag tgaaggcgga
    31621 tattgccggt ggcggccttt tccagcagct tgtcctggag gatcttctcg gcgcgcagct
    31681 tgtcgcgacg atggatcagg tggacttctt tggcgatgtt ggacaggtac agcgcctctt
    31741 cgacggcggt gttgccaccg ccgatcacgc agaccacctg gttgcgatag aagaagccat
    31801 cgcaggtggc gcaggcggat acacccttgc ccatgaaggc ttcttcggag ggcaggccca
    31861 ggtactgggc agaagcgcca gtggcgatga tcagggcatc gcaggtaaag gtgccgctgt
    31921 cgcccttgag cacgaacggg cgctgttgca actcggcggt gtggatgtgg tcgaacacga
    31981 tatcggtggc aaagcgttcg gcatgctcgc gcatgcgctc catcagcgcc ggtccggtga
    32041 ggccttcgac gtcgccgggc cagttgtcga cctcggtggt ggtggtcagc tggccgccag
    32101 cctgcaggcc ggtgatcagc agcggcttga ggttggcgcg cgcagcgtag acggcagccg
    32161 tgtagccggc cgggccggaa ccgaggatga tcagccgcga atgcttggcg tcactcataa
    32221 aacaccccat aagtctttgt cacgaaagag aatgcatgct ccaattgagc tgccatgcgg
    32281 tcaatgcgga gcatgctaca cccctctggc tggggcggca aaacccggcg ttgacggcct
    32341 tcatggcatc ctccgggtaa agtcttacaa tagtctctga ttttttctct accgatttcg
    32401 agcgccggac tagcgcagga aaaacacgtt ttgaagagct ctgccaatac cgccaaagcc
    32461 gatgccttgc gccagcagct gcattaccgt ctgaaggaag cagcgctgat tgcgctgggg
    32521 gcgctgtgca tctatttctg gatggctctg ttgagctact ccccgatgga tccgggcatc
    32581 gatgtctcca ccagtgcggg ccatgtgcag aatgcggccg ggcgggtagg ggcctgggtg
    32641 gccagtttct tcctgttcgg cttcggctat tcggcctacc tgctgccgct gctgatgggt
    32701 atcaaggttc tcgagctgtt ccgcacgcgc catgagccgt tccgctggaa tggctggctg
    32761 ttttcctggc ggatcatcgg cctgttcttc ctgctgacct cgacggcagc cctggcttcc
    32821 atccagttcc aggcgctgga aatgaatccg caggcgccgg ccggcgggat tcttggcaag
    32881 agcctcggcg agtgggccga ggacgccttg aatgtgcagg gcagcacgct gatgttcatc
    32941 gcgctgttcc tcttcgggct gaccgtgttc accgatctgt cgtggttccg ggtcatggac
    33001 ctcactggca agatcaccct tgacctgttc gagctgattc agggtgccgg ccaccgctgg
    33061 tgggagggcc gccagacgca tcggcagctg aagctgaagt tgcgagagtc tgacgaggcg
    33121 gtcgagcccg cggcggaacc ggtatccggg cggccacgct cggcagccag tgccccggct
    33181 gcgcccgtcg ccagggcggt gccggcaggt gtcgcggcg
//
LOCUS       DATCUN010000056         7296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_77429, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000056 DATCUN010000000
VERSION     DATCUN010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77429"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..1334
                     /locus_tag="VIO83_02045"
     CDS             396..1334
                     /locus_tag="VIO83_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017136244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HEY8330101.1"
                     /translation="MAGSSLLALIDDIATILDDVSVMTKVAAKKTAGVLGDDLALNAQ
                     QVTGVNADRELPVVWAVAKGSFINKLILVPAALLISALAPWAVTPLLMLGGAFLCFEG
                     FEKLAHKFLHSQAEEAGKHAELEVALANPAVDMVAFERDKIKGAVRTDFILSAEIIAI
                     TLGAVAGAPFMQQVVVLAGIAIVMTIGVYGLVAGIVKLDDLGLHLSRRASSAAQAVGG
                     LILRAAPYLMKGLSVVGTAAMFMVGGAILTHGMAPVHHLIENAAEATGAVAGIGALLQ
                     ALTPTLLNALFGIAAGGVVLAAVNLFGALRSRLKQA"
     gene            complement(1385..2728)
                     /locus_tag="VIO83_02050"
     CDS             complement(1385..2728)
                     /locus_tag="VIO83_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEY8330102.1"
                     /translation="MKNIKLSYLLLLIVLSLLWLVADNVLTAPYQFFALRSSLVNYTG
                     ILAIGVMSFGMFLAIRPISIEPFLGGLDKSYRLHKWLGITALVLSVVHWLLIKAPKWL
                     IGWGWMEKPVRKAATAAGPSEPIPAIFQFFRSQRGLAEDLGEWAFYAVVALIVLALLK
                     RFPYRYFFKTHRLLAVVYLVLVFHSLVLMKTAYWSSPIGPLVAVLMLVGTLAAFVSLL
                     RRVGHTRRAVGVIEQLVHHRDNRVLGVEIKLKDRWFGHAAGQFAFVTFDPAEGPHPFT
                     ISSSWHNDGKLRFHIKGIGDYTATLPQTLKVGDLVKVEGPYGTFNFRSKQPRQIWVAG
                     GIGITPFIARMQSRERHPEERSIDLFYSTSAPDQGFIDKLQQLAKRANVQLHVLVSGK
                     DGRLTADRLCQMVPEWQSASVWFCGPAGFGQSLRQDLLARGLAAADFHQELFDMR"
     gene            complement(2853..5939)
                     /locus_tag="VIO83_02055"
     CDS             complement(2853..5939)
                     /locus_tag="VIO83_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8330103.1"
                     /translation="MSFPNLSALAVRERSVTLFFLILAVLSGIYAFLALGRAEDPAFS
                     VRATVVTAVWPGATPEELQTQVVDRLEKRIQEVEYLYRIETTIRPGQANLQIEFHDYS
                     PQAKIPDLLYQVRKRMQEEAANLPPGVIGPIVNDDFSDVYFSLIAITAPGLPMRELTR
                     DAEAIRDRLQRVAGVHKAMLLGERTERVYLEFDNARLINLGIAPQAVFDAIDASNRLL
                     PAGRMETDGPRLHLRLDADLADLEQLATVPVRIGERVLKLADIATIRRGYEDPPSYLV
                     RSRGQDALLLGVVMAKGENGLELGERLADFLDAERQRLPLGMALDVLTNQAEAISGAV
                     NLFQVKFLVAVAVVVAVSMLAIGLRAGLIVGIAVPLTLGLTFLVMMLMGINLDRITLG
                     ALIIALGLLVDDAIIAVEMMLVKMEEGWDRVRAAAHAWSVTAAPMLFGTLVTVAGFFP
                     IGFARSSVGEYAGNIFWVLAIALVVSWLVAVVFAPWLGVRMLPDFPKAHDHAGAYRTP
                     LYRRLRSVVTWCVTRRKTVVGATVALLVLSIAGMALLVQKQFFPSSDRPEVLVAVYLP
                     QGSSVAATDRTIKKLEAILAPLPEVKSLSAYVGAGAPRFFISANPEPPSPAFAKIVAV
                     AQDPKARDKVMATLQQHIDRGEFPEARVRVYQLLFGPPVIWPVAFRVIGPDPVELRGI
                     AHRVRTVMAADPHVVDPHLEWDERAPALHLAMDEERLRLLGLTPQEVAQQLQFQLDGV
                     PVTQLRQDIRSVVVIARGSSGGRRLDADSLDTLEVLTRDGRKLPFSQLGQVEVRFEDP
                     VIKRYNREPFLTVQADIRDAQPNDVTAAVWQAMEDVRGRLPPGYRIDIAGSVEESGKA
                     DASIQKLQPAMMAFMLILIMLQMRSFSGTFIVLATAPLGLIGAVIALLLFDQPFGFVA
                     LLGLTGLAGILMRNTLILTQQVADNFDAGMAAFEGVVEAAVQRARPVMLTALAAVFAF
                     VPLTQDSFWGPLAYVLIGGVAVGTAITLLFVPALYALWFRLAPAASAEGSLPSSGM"
     gene            complement(5936..6901)
                     /locus_tag="VIO83_02060"
     CDS             complement(5936..6901)
                     /locus_tag="VIO83_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8330104.1"
                     /translation="MPPFVKTVAVLGSSAATLGLSGTVRAQVESPLAFQVGGRIAKRA
                     IDAGQSVRAGQVLFELDTRDLTQAVSAAEADLAAATAALATAEADLVRHRQLEARNFV
                     SAQALERVQLARREAQTRRDASTAHLAQARNALGYGRLRAPAAGVLIEVTGEAGQVVA
                     AGLPVALLAQADAREIEVYFPEGVTPPDRGEVLAADGTTLPLRLRETAGAVDPQGRTL
                     RARYTVLEQPDALVLGTVLRTRFAAQAANEASFSVPIGAIDERSQGPRVWRVREGRAV
                     SVSVSVLALDGETARIRGPLVGDDRVVALGTHLLSENMPVRELGQ"
     gene            7071..>7296
                     /locus_tag="VIO83_02065"
     CDS             7071..>7296
                     /locus_tag="VIO83_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8330105.1"
                     /translation="MSAKAGRPTDHQKDRDILHAARELVFAEGPQALTMDKVASLAGI
                     SKVTLYARYANRYALLQAVVSSEAFGIHKAL"
BASE COUNT         1278 a         2346 c         2502 g         1170 t
ORIGIN      
        1 cgccagtagc gagaatttct ctacccggcg gcgcggggcg ggattctatc gaagatggtt
       61 caaaaggtcg gcagctcgta tctctttatg gctgctcatt tttttcttga gtataggagg
      121 gtggcgggca tcctcaaggc agctgcggat ttgcctgact cggtacgctg ctaaatacgc
      181 tatttaacat aatgaaatga tgctgtttct tgcctattcc cgtggccttg ttgcgatcga
      241 acaggcgatc aatggtgagc cctggcttgc atcgaagagg agtgcatccc tgtcggcaga
      301 tgggaaattt tgtctgcgcg gaaaatgacc ggtaacggct tagccgttat gctcgcgcga
      361 ttccatccgg aaattgccat gcagggagaa ccaacttggc tggaagtagc ttgcttgcac
      421 tgatcgatga catcgccacg atcctggacg acgtatcggt aatgaccaag gtggcggcga
      481 agaagaccgc cggagtgctc ggtgatgacc tggcgctgaa cgcccagcag gtcaccggcg
      541 tcaatgccga ccgcgagctg ccggtggtct gggccgtggc caagggctcg ttcatcaaca
      601 agctgatcct ggtgccggcg gcgctgctga tcagcgcgct ggctccctgg gcggtcactc
      661 cgctgctgat gctcggcggc gcgttcctgt gtttcgaggg cttcgagaag ctcgcgcaca
      721 agttcctgca cagccaggca gaggaggctg gcaaacacgc cgagctggag gtggccctgg
      781 ccaatcccgc ggtcgacatg gtggcgttcg agcgggacaa gatcaaaggc gcggtgcgca
      841 cggacttcat cctgtccgcg gagatcatcg caatcaccct gggcgcggtc gccggtgcgc
      901 cgttcatgca gcaggtcgtg gtgctggcag gaatcgccat cgtcatgacc atcggcgtct
      961 acggcctggt ggccggtatc gtcaagctcg atgacctggg cctgcacctc agtcgtcggg
     1021 cctcgagcgc ggcgcaggcg gtcggcgggc tgatcctgcg tgccgcccct tatctgatga
     1081 aggggctgtc ggtggtcggt accgcggcca tgttcatggt cggtggcgcc atccttaccc
     1141 acgggatggc gccggtgcac cacctcatcg aaaacgccgc cgaggcaacc ggcgccgtgg
     1201 cgggtattgg cgcactgctg caggcgctga ccccgacgtt gctcaatgca ctgttcggca
     1261 ttgccgccgg tggcgtggtg ctggctgccg tcaatctgtt cggggctctg cgcagtcgct
     1321 tgaaacaggc ctgactcact gcggcctgcc gcctgcgcgt tggcgggcgg caggcgcggc
     1381 gctctcagcg catgtcgaac agctcctgat ggaagtccgc ggccgccaga ccgcgggcga
     1441 gcagatcctg acgcagcgac tggccgaagc cggccggccc gcagaaccag acgctggccg
     1501 attgccattc gggaaccatc tgacacaggc gatcagccgt cagccggccg tccttgccgc
     1561 tgaccagcac gtgcagctgc acgttcgcgc gtttggccaa ttgctgcagc ttgtcgatga
     1621 agccctggtc cggcgcgctg gtgctgtaga acaggtcgat gctgcgttct tccggatgac
     1681 gctcacggct ctgcatgcgg gcgatgaacg gggtgatgcc gataccgcca gccacccaga
     1741 tctgtcgcgg ctgcttgctg cggaagttga aggtgccgta agggccctcg accttgacca
     1801 ggtcgccgac cttgagggtt tgcggcaggg tcgcggtgta gtcgccgatg cccttgatgt
     1861 ggaagcgcag cttgccgtcg ttgtgccatg acgacgaaat ggtgaagggg tgcgggcctt
     1921 cggccggatc gaaggtgacg aaggcgaatt gcccggcggc atgcccgaac cagcggtctt
     1981 tcagcttgat ctcgacgccc agcacgcggt tgtcgcgatg gtgcaccagt tgctcgatca
     2041 cgccgaccgc gcgacgcgta tggcccaccc ggcgcaacag cgagacgaag gcggcgaggg
     2101 tgccgaccag catcagcacc gccaccagcg ggccgatggg cgaactccag taggcggtct
     2161 tcatcagcac cagcgagtgg aacaccagca ccaggtagac caccgccagc aatcgatggg
     2221 tcttgaagaa gtagcgatag ggaaagcgct tgagcagcgc caggacgatc agggcgacca
     2281 ccgcatagaa cgcccactcg ccgaggtctt cggccagacc acgctggctg cggaagaact
     2341 ggaaaatggc cgggatcggt tcgctcggcc ccgcggccgt ggctgccttg cgcaccggct
     2401 tttccatcca gccccagcca atcaaccact tcggcgcttt gatcagcagc cagtgcacga
     2461 cggaaagcac cagggcggtg atgcccagcc acttgtgcag ccggtaggac ttgtccagcc
     2521 cgcctagaaa cggctcgatg ctgatcgggc gaatcgccag gaacatgccg aagctcatga
     2581 cgccgatggc gagaatgccg gtgtagttga ccagcgacga gcgcagggcg aagaactggt
     2641 agggcgcggt cagcacgttg tcggccacca gccagagcag gctgaggacg atcagcagca
     2701 ggagatagct cagcttgatg ttcttcatag ggcaaatcct ttcaatcctt ttcgaagagg
     2761 tgtgcgttgt atagcgtaac cgctcaagga cggtgttgcg ctgctgcaag aggacgcagt
     2821 gcgagcccgc aatccatggc agccacgagc gtctacatcc cggagctcgg caagctgcct
     2881 tccgcgctgg cagcaggcgc gagacggaac cacagcgcat agagcgccgg cacgaacagc
     2941 agggtgatcg ccgtaccgac cgccacgccg ccgatcagca cgtaggctag cggcccccag
     3001 aaactgtcct gggtgagggg gacgaaggcg aacacggcgg ccagcgccgt caacatcact
     3061 gggcgggcgc gctgcacggc cgcctctacc accccctcga acgccgccat gcccgcgtcg
     3121 aaattgtcgg ccacctgctg ggtcaggatc agggtgttgc gcatcaggat gccggccagg
     3181 ccggtcaggc cgagcagggc gacgaagccg aacggctggt cgaacagcag cagggcgatg
     3241 accgcgccga tcaggcccag cggggcggtc gccaggacga tgaaggtgcc ggagaaggag
     3301 cgcatctgca gcatgatgag aatcagcatg aacgccatca tcgctggctg cagtttctgg
     3361 atcgaggcat cggccttgcc cgactcctcg accgagccgg cgatgtcgat gcggtagccc
     3421 ggcggcaagc ggccgcgcac atcttccatg gcctgccaca cggcagcagt cacgtcgttg
     3481 ggctgggcat cgcgaatgtc ggcctgcacg gtcaggaacg gctcgcggtt gtagcgcttg
     3541 ataaccgggt cctcgaagcg cacctcgacc tgccccagct ggctgaacgg cagcttgcgg
     3601 ccgtcgcggg tcagcacctc cagggtgtcc agactgtcgg cgtcgagccg gcggccgccg
     3661 ctgctgccac gggcgatgac caccacgctg cggatgtcct ggcgcagctg ggtgaccggc
     3721 acgccgtcga gctggaactg cagttgctgc gccacttcct gcggggttag ccccagcagg
     3781 cgcaggcgct cctcgtccat ggccagatgc agcgccgggg cgcgctcgtc ccattccagg
     3841 tgcgggtcga ccacatgggg atcggccgcc atcacggtgc ggacccgatg ggcgatgccg
     3901 cgcagctcga ccggatcggg gccgatgacg cggaaggcga ccggccagat caccggcggg
     3961 ccgaacagca gctggtacac ccgcacccgc gcctcgggga actcgccccg gtcgatgtgc
     4021 tgctgcaggg tggccatgac cttgtcccgc gccttgggat cctgcgccac ggcgacgatc
     4081 ttggcgaagg cgggcgaggg cggctcgggg ttggcgctga tgaagaagcg cggcgccccg
     4141 gccccgacat aggcggacag gctcttgacc tccggcagcg gggcgaggat ggcttcgagc
     4201 ttcttgatcg tgcgatccgt ggcggcgacg ctgctgccct gcggcaggta gaccgcgacc
     4261 agtacttccg ggcggtccga gctggggaaa aactgcttct gcaccagcag ggccatgccg
     4321 gcgatggaca gcaccagcaa cgctacggtg gcgccgacca ccgtcttgcg ccgggtcacg
     4381 caccaggtca cgacgctgcg caggcgccgg tacagcgggg tccgataggc gccggcgtgg
     4441 tcgtgggcct tgggaaaatc cggcagcatc cgcacgccca gccagggcgc gaacaccacc
     4501 gcgaccagcc aggacaccac cagcgcgatg gccaacaccc agaagatgtt gccggcgtat
     4561 tcgccgacgc tcgagcgggc gaagccgatg gggaagaagc cggccacggt caccagggtg
     4621 ccgaagagca tgggtgcggc ggtgaccgac caggcatggg cggcggcgcg cacgcggtcc
     4681 cagccttcct ccatcttcac cagcatcatt tccacggcga tgatggcgtc gtcgaccagc
     4741 aggcccaggg cgatgatcag cgcgcccagg gtaatgcggt cgaggttgat gcccatcagc
     4801 atcatcacca ggaaggtgag gcccagggtc agcggcacgg cgatgccaac gatcagcccg
     4861 gcgcgcaggc cgatggccag catgctgacc gccaccacca cggccacggc gacgaggaac
     4921 ttgacctgga acaggttgac cgccccgctg atggcctcgg cctggttggt cagcacgtcg
     4981 agcgccatgc ccagcggcag tcgctggcgt tcggcgtcga ggaagtcggc gagccgctcg
     5041 cccagctcca ggccgttttc gcccttggcc atgaccacgc cgagcagcag cgcatcctgc
     5101 ccgcgcgagc gcaccaggta gctgggcggg tcctcgtagc cgcgcctgat ggtggcgatg
     5161 tcggcgagct tgagcacccg ctcgccgatg cgcaccggca ccgtggccag ctgctcgaga
     5221 tcggcgaggt cggcgtcgag gcgcagatgc aggcgcgggc cgtcggtctc catgcgtccc
     5281 gccggcagca ggcgattgct ggcgtcgatg gcgtcgaaca ccgcctgcgg ggcgatcccc
     5341 aggttgatca agcgggcatt gtcgaactcg agatacaccc gctcggtgcg ctcgcccagc
     5401 agcatggcct tgtgcacccc ggcgacgcgc tgcaggcgat cgcggatcgc ctcggcgtcg
     5461 cgggtcagtt cgcgcatcgg caggcccggg gcggtgatgg cgatcaggct gaagtacaca
     5521 tcggagaagt cgtcgttgac gatcgggccg atcaccccgg gcggcaggtt ggcggcctct
     5581 tcctgcatgc gcttgcgcac ctggtagagc aggtcgggga tcttggcctg cggcgagtag
     5641 tcgtggaact cgatctgcag gttggcttgc ccgggacgga tggtggtctc gatccggtag
     5701 aggtactcca cctcctggat gcgcttctcc aggcggtcga ccacctgggt ctgcagttcc
     5761 tcgggcgtgg cgccgggcca gaccgcggtg acgaccgtgg cccgcacgct gaaggcgggg
     5821 tcttccgccc ggccgagggc caggaaggcg tagatgccgg acagcacggc gaggatcagg
     5881 aaaaacagcg tcaccgagcg ctcgcgcacg gccagggcgg acaggttggg gaagctcatt
     5941 ggccgagttc ccgcacgggc atgttttcac tcagcagatg ggtgcccagc gccacgacgc
     6001 ggtcatcgcc taccagcggg ccgcggatgc gcgccgtttc gccatccagc gccagcacgc
     6061 tgaccgaaac ggacactgca cgaccctcgc ggactcgcca gacccgcggt ccctggctgc
     6121 gctcatcgat ggcgccgatg ggcaccgaga aactggcttc attcgccgcc tgcgcggcga
     6181 agcgggtacg caataccgtt ccgagcacca gggcatccgg ctgctccagc accgtgtagc
     6241 gggcgcggag ggtgcgtccc tgcggatcga cggcgccggc cgtttcgcgc aggcgcagcg
     6301 gcagggttgt cccgtcggca gccaagacct cgccgcggtc cggcggtgtc acgccctccg
     6361 gaaagtacac ctcgatctcg cgcgcatcgg cctgggcgag gagggccaca ggcaggcctg
     6421 cggccaccac ctggccggcc tcgccggtta cctcgatcag cacgccggca gccggcgccc
     6481 gcagtcggcc gtagccgagg gcattgcggg cctgggccag gtgcgcggtg ctggcgtcac
     6541 gccgggtctg ggcttcgcgg cgggcaagct ggacacgctc cagggcttgc gcgctgacga
     6601 agttccgcgc ctccagctgg cgatggcgga ccaggtcggc ttcggcagtg gcgagcgcgg
     6661 cagtggcggc ggccaggtcc gcttcggccg cgctgaccgc ctgggtcagg tcacgggtat
     6721 ccagctcgaa cagcacctgc ccggcgcgca cggactgccc ggcatctatc gcgcgcttgg
     6781 caatccgccc gcccacctgg aaggctagcg gcgactcgac ctgcgcgcgc accgtacccg
     6841 acaggcccag cgtcgctgcg gaactgccga gtaccgctac tgtcttgacg aagggcggca
     6901 tggctgccgc ggtcttgggc tcagggctat cattgcaagc tgcgagaaat atcgagcatg
     6961 cagccagcaa aagtagcgaa atacgcatga caagtcgttc ccctgattag tgaacacgaa
     7021 tgtacagaaa tcatttttgc tcatccagca ccgagtgcag gcgcgaaacc atgtcagcaa
     7081 aagccggacg ccccaccgat caccagaagg atcgcgacat tctccacgct gcccgggagc
     7141 tggtgttcgc tgaggggccg caagcgctca ccatggacaa ggtggcgagc ctggcgggca
     7201 tctccaaggt gacgctgtat gcacgctacg ccaaccgcta tgcgctgttg caggcggtgg
     7261 tgagcagcga ggcgttcggc atccacaagg cactcg
//
LOCUS       DATCUN010000057         8928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_171422, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000057 DATCUN010000000
VERSION     DATCUN010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171422"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /gene="xseA"
                     /locus_tag="VIO83_02070"
     CDS             complement(<1..541)
                     /gene="xseA"
                     /locus_tag="VIO83_02070"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY8330106.1"
                     /translation="MIKDPFARLGLDREVLTVSQLNQRARHLLEDVFPQVWVEGEISN
                     LARPSSGHVYFTLKDAGAQVRCALFRSSAARVRQALRDGLAVKVRGKVSLFEGRGDYQ
                     MILDTLEPAGDGALRLAFEALKDKLAGEGLFVAEGKRALPAHPQRIGIVSSPSGAVIR
                     DIISVFRRRAPQVQLTLIPT"
     gene            640..1191
                     /locus_tag="VIO83_02075"
     CDS             640..1191
                     /locus_tag="VIO83_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATPase"
                     /protein_id="HEY8330107.1"
                     /translation="MADQQLIIVPQLSSVPAHEAKARQLLNWLVKREIVEALPTTCGR
                     GGNGMAYAIAPGARRVVEHPQLLPFGQPHNGLEIVTQRCIYTPTRDFLEEAGCPHCRQ
                     EIGEPLFASLDEWWPGYTDNFTCPECGFEDDINGFLFLQPCGFSNLGFIFNGWAEAGL
                     RQDFLAAFAERLGFKIRVVQVQL"
     gene            1269..1850
                     /locus_tag="VIO83_02080"
     CDS             1269..1850
                     /locus_tag="VIO83_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY8330108.1"
                     /translation="MAAKKILMLVGDYVEDYEVMVPFQALTMVGHLVHAVCPGKSAGQ
                     SVRTAIHDFEGDQTYSEKPGHNFVLNFDFAKVEAADYDALLVPGGRAPEYLRLNGQVI
                     ELVQALAQAGKPIAAICHGAQLLAAAGVLKGRECSAYPACAPEVGLAGGRYVEIPVDQ
                     AHTDGNLVSAPAWPAHPAWLAAFLKVLGTRIEL"
     gene            1984..2292
                     /locus_tag="VIO83_02085"
     CDS             1984..2292
                     /locus_tag="VIO83_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEY8330109.1"
                     /translation="MCELYVKADPILYESRSRSLRIRGVVTTLRLENQFWDILREIAE
                     VDGMTTNQLIAKLYEEVMDYRGEVVNFASFLRVSCTRYLAQRRDKVAELSLVAARAVS
                     "
     gene            2383..3852
                     /gene="guaB"
                     /locus_tag="VIO83_02090"
     CDS             2383..3852
                     /gene="guaB"
                     /locus_tag="VIO83_02090"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY8330110.1"
                     /translation="MLRISQEALTFDDVLLIPGYSEVLPKDVSLKTRLTRGIELNIPL
                     VSAAMDTVTESRLAIAMAQEGGIGIIHKNMTIEQQAAEVRKVKRHETAIVRDPETVTP
                     ETKISELLRKADELGFSGFPVVSGKELVGIVTGRDLRFTPNTGDSVAAIMTPKDQLVT
                     VQEGTGLEEIKTKLYEHRIEKMLVVDSQFHLRGLVTFRDIEKAKTYPLASKDDQGRLR
                     VGAAVGTGADTGDRIAALAAAGVDVVVVDTAHGHSKGVIDRVRWVKQNFPEVQVIGGN
                     IATAEAALALVEAGADGVKVGIGPGSICTTRIVAGVGVPQISAIANVATALAAVGVPM
                     IADGGIRFSGDLSKAIVAGANAVMMGSMFAGTEEAPGEVELFQGRSYKAYRGMGSLGA
                     MAQAQGSSDRYFQDSSAGAEKLVPEGIEGRVPYKGALSAIIHQLMGGLRSSMGYTGCA
                     NIEEMRTRPQFVRITGAGMAESHVHDVQITKEAPNYRVG"
     gene            4077..5657
                     /gene="guaA"
                     /locus_tag="VIO83_02095"
     CDS             4077..5657
                     /gene="guaA"
                     /locus_tag="VIO83_02095"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY8330111.1"
                     /translation="MAHHDIHAHRILILDFGSQYTQLIARRVREIGVYCEIHPFDMDD
                     AAIRAFAPRGIILAGGPESVHEADSPRAPQAVFELGVPLFGICYGMQTMAEQLGGKVQ
                     GSDLREFGYARVDLVGKSRLLDGIEDHMADDGMLALDVWMSHGDKVTALPEGFHIVAS
                     TPSCPIAAMADDSRAYYGVQFHPEVTHTKQGGRILSRFVLDLCGCEALWTPANIVDDA
                     IANVRAQVGDSKVLLGLSGGVDSSVVAALLHRAIGDQLTCVFVDNGLLRLHEGDQVMT
                     MFAENMGVKVIRANAEDKFLGRLAGISDPEEKRKIIGRSFIEVFDEEATKLQGIDFLA
                     QGTIYPDVIESAGAKTGKAKVIKSHHNVGGLPEDMQFKLVEPLRELFKDEVRKIGLEL
                     GLPYDMVYRHPFPGPGLGVRILGEVKKEYADLLRRADHIFIEELRNFDWYHKTSQAFV
                     VFQPVKSVGVVGDGRRYAWVVALRAVETIDFMTARWAHLPYELLEKVSNRIINEIEGI
                     SRVTYDVSSKPPATIEWE"
     gene            6049..6567
                     /locus_tag="VIO83_02100"
     CDS             6049..6567
                     /locus_tag="VIO83_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HEY8330112.1"
                     /translation="MPTPAYLSIEGTKQGLITAGTFTEGSVGNIFQEGHEDQILVEAF
                     QHQVIIPRDPQSGQPTGQRVHKPLMITKVFDKSSPLLFNALTSGERLTKCRLEWYRTS
                     ATGTQEHYFTIELHDAIIVDVQSRMPNCQDPNMAHFTHLEDVYFTYRKIVWTHEVSGT
                     SGSDDWRTPISV"
     gene            6892..8133
                     /locus_tag="VIO83_02105"
     CDS             6892..8133
                     /locus_tag="VIO83_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003300877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEY8330113.1"
                     /translation="MKALALGLLLLAALLYALATHLLALHPAWGYLAAFAEAAMVGAI
                     ADWFAVTALFRHPLGLPIPHTAIIPRKQARLGRNLADFICTHFLATPQVMAKLEAFDA
                     AGRLASWLRQPANADAVGRQLVGVARYGIGALRDERVRQFIQRTATAKLREVDLACVG
                     GQLLDMLTHQRRHQAMLDEVLARLDELLQDEAVQQRIAAGLSSEFSALRFRLFGKDIG
                     LDEKAGQWSADKVVRRVSALIGEISRDSQHELRGKFDACVAVLIGKLKDDPDFRLKGE
                     QIREQLLAHPAVAGYLRKLWDDLVDWLQADLADADSTIRRRLIRLCQRLGEALAADEA
                     MRRWLNDQLLEVAPTLVERHREDIRRYIAERVEKWNSDELVAQLEYNVGKDLQFIRIN
                     GTLVGGLVGLVLHAVTRLLAS"
     gene            8298..>8928
                     /locus_tag="VIO83_02110"
     CDS             8298..>8928
                     /locus_tag="VIO83_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY8330114.1"
                     /translation="MSFTRRQVLTGLAGLGVLGLAGGGARYWLGRPAATTTHDYELIA
                     APLDVELVPGHQTRAWAYGGQLPGQELRVRQGDWLRVRFINRLEVPSTIHWHGIRLPL
                     AMDGVPYVSQLPVLPGEYFDYRFQVPDAGSFWYHPHTASGEQLGRGLVGPLIVEEREP
                     SGFRHERTLSLKSWHVDEQGAFSDFSVPREAARGGTAGRLSTVNGEHAPT"
BASE COUNT         1483 a         3039 c         2929 g         1477 t
ORIGIN      
        1 ccgtggggat caaggtgagt tgcacctgcg gcgcgcggcg gcggaacacg ctgatgatgt
       61 cgcggatcac cgcgccgctc ggcgagctga cgatgccgat gcgctgcgga tgagcgggca
      121 gggcgcgctt gccctcggcg acgaacaggc cctcgccggc cagcttgtcc ttgagcgcct
      181 cgaaggccag gcgcagcgcg ccgtcgccgg ccggctccag cgtgtcgagg atcatctggt
      241 agtcgccgcg gccctcgaac agcgagacct tgccgcgcac cttcaccgcc aggccgtcgc
      301 gcagcgcctg gcgcacgcgc gccgcgctgg agcggaacag cgcgcagcgc acctgggcgc
      361 cagcgtcctt gagggtgaag tagacgtgcc cggaagaggg ccgcgccagg ttggaaatct
      421 cgccctcgac ccacacctgc gggaacacgt cctcgagcag gtggcgggca cgctggttga
      481 gctggctgac ggtcagcacc tcgcgatcga ggccgagccg tgcgaacggg tctttgatca
      541 tggccggcat gataaaggct ttgccgggac taccataggc attgacctgc gccggccgcc
      601 gattaagctg cgcggctttc ttcacgccga gggcattcca tggccgacca gcagctcatc
      661 atcgtcccgc agctttccag cgtccccgcc cacgaggcga aggcgcggca gctgttgaac
      721 tggctggtca agcgcgagat cgtcgaggcg ctgccgacca cctgcgggcg cggcggcaac
      781 ggcatggcct atgccatcgc tccgggagcg cggcgggtgg ttgagcatcc gcagctgttg
      841 ccgttcggcc agccgcacaa cggcctggaa atcgtcaccc agcgctgcat ctatacgccg
      901 actcgcgact ttctcgagga ggccggctgt ccgcactgcc gccaggaaat cggcgagccg
      961 ctgttcgcca gcctggatga gtggtggccg ggatataccg acaatttcac ctgcccggaa
     1021 tgcggcttcg aggacgacat caacggtttc ctgttcctgc agccgtgcgg cttctccaat
     1081 ctgggcttca tcttcaacgg ctgggcggag gcgggcctgc gccaggactt cctcgctgcc
     1141 ttcgccgagc gcctgggctt caagatccgc gtggtgcagg tacagctgta gtaggcggct
     1201 gttacccgtt tgctgcgggc tggacgatac tgggtgcact tcaatcaccc ggaacaggga
     1261 gcaagtccat ggctgcgaag aagatcctca tgctggtcgg cgactacgtc gaagactatg
     1321 aagtgatggt gccgttccag gcgctgacga tggtcggtca cctggtgcat gcggtgtgtc
     1381 cgggcaagag cgccgggcag agcgtgcgca ccgcgatcca cgacttcgag ggcgaccaga
     1441 cctacagcga gaagccgggg cacaacttcg tcctcaactt cgacttcgcc aaggtcgagg
     1501 ccgccgacta cgacgccctg ctggtgccgg gcggccgcgc gccggagtac ctgcgcctga
     1561 acggtcaggt gatcgaactt gtgcaggccc tcgcccaggc cggcaagccg atcgccgcca
     1621 tctgccacgg cgcgcagctg ctggctgctg cgggcgtgct caagggccgc gaatgcagcg
     1681 cctacccggc ctgcgcgccg gaagtcggcc tggccggcgg gcgctacgtg gagatcccgg
     1741 tcgaccaggc gcacaccgac ggcaatctgg tcagcgcccc ggcctggccg gcgcatccgg
     1801 cctggctggc ggcgttcctc aaggtgctcg gcacccgtat cgaactgtaa tccgcgccgc
     1861 gggacggtag aaaatcgctg cgcgattttt ctaccctacg gaggctgcgc cgttagctgg
     1921 tagggtggac aacggcgcag ccttgtccac caccccggcg atgccaatga ggggaaaaca
     1981 gtcatgtgcg agctgtacgt caaggccgat ccgatcctct acgagtcgcg ctcgcgctcc
     2041 ctgcgcatcc gcggggtggt caccaccctg cgcctggaga accagttctg ggacatcctc
     2101 cgcgagatcg ccgaggtcga cggcatgacc accaatcagc tgatcgccaa gctgtacgag
     2161 gaggtcatgg actaccgcgg cgaggtggtc aacttcgctt ccttcctgcg cgtcagctgc
     2221 acccgctacc tggcccagcg tcgcgacaag gtcgcggagc ttagtctggt cgctgcccgg
     2281 gcggtgtcct gagcggcgag ttaacgacca gccgcgttga gcgcgggcgc tgggccgggt
     2341 atactgccgc gcttccattt ttcccgtccg ggagcccccg ccatgctgcg tatcagccaa
     2401 gaagccctga ccttcgacga tgttctattg atccccggtt actccgaggt tctgccgaag
     2461 gatgtctccc tcaagacccg tctgacccgc ggtatcgaac tgaacatccc gctggtttcc
     2521 gcagccatgg ataccgtgac cgagtcccgc ctggccatcg ccatggccca ggaaggcggc
     2581 atcggcatca tccacaagaa catgaccatc gagcagcagg ctgccgaggt gcgcaaggtc
     2641 aagcgtcacg agaccgccat cgtgcgcgat ccggagaccg tgaccccgga aaccaagatc
     2701 agcgagctgc tgcgcaaggc cgacgagctg ggcttctccg gcttcccggt ggtgtccggc
     2761 aaggaactgg tgggcatcgt caccggccgt gacctgcgct tcaccccgaa taccggcgac
     2821 agcgtcgcgg cgatcatgac gccgaaggac cagctggtca ccgtgcagga aggcaccggc
     2881 ctggaagaga tcaagaccaa gctctacgag caccgcatcg agaagatgct ggtggtcgac
     2941 agccagttcc acctgcgtgg cctggtgacc ttccgcgaca tcgagaaggc caagacctac
     3001 ccgctggcgt ccaaggatga ccagggtcgc ctgcgcgtcg gcgccgccgt cggcaccggt
     3061 gccgacaccg gcgaccgcat cgccgcgctg gctgccgccg gcgtcgacgt ggtggtggtg
     3121 gataccgccc acggccactc caagggcgtg atcgaccgcg tgcgctgggt caagcagaac
     3181 ttccccgaag tgcaggtgat cggcggcaac atcgccaccg ccgaagccgc cctggcgctg
     3241 gtcgaagccg gcgccgacgg cgtcaaggtc ggcatcggcc cgggctcgat ctgcaccacc
     3301 cgcatcgtcg ccggtgtcgg cgtgccgcag atcagcgcca tcgccaacgt cgccaccgcc
     3361 ctggccgcgg tcggcgtgcc gatgatcgcc gacggcggca tccgcttctc cggcgacctg
     3421 tccaaggcca tcgtcgccgg cgccaacgcg gtgatgatgg gctcgatgtt cgccggtacc
     3481 gaagaagcgc cgggcgaggt cgagctgttc cagggccgct cctacaaggc ctaccgcggc
     3541 atgggctcgc tgggcgccat ggcccaggcc cagggttcct cggaccgcta cttccaggac
     3601 tcctcggccg gtgccgagaa gctggtcccg gaaggcatcg aaggccgcgt accgtacaag
     3661 ggtgccctga gcgcgatcat ccaccagctg atgggtggcc tgcgttcctc catgggttac
     3721 accggctgcg ccaacatcga agagatgcgc acccgcccgc agttcgtgcg catcaccggc
     3781 gccggcatgg ccgagtcgca cgtgcacgat gtgcagatca ccaaggaagc ccccaactac
     3841 cgcgttggtt gaaaaatcga tttgacgctg ctgcgcttgg ccatgctgcg ttggaaagag
     3901 gttcggaatg ctcatttgct ccagcaaact ccgcttcctc acctctttcc gccttgcctg
     3961 tcctgcgctc gcgacgcggc aaatcgattt ttgcctgatt gagaaagacc cggggttgcc
     4021 gctgtaacag gcgggcagcc ccgagtcgtt tgtgaattcc gctacgagag acggctatgg
     4081 cccaccacga cattcacgcc caccgtatcc tgattctgga cttcggctcc cagtacaccc
     4141 agctgatcgc ccgccgcgtg cgcgagatcg gcgtgtactg cgagatccac ccgttcgaca
     4201 tggacgacgc ggccatccgc gccttcgcgc cgcgcggcat catcctcgcc ggtggtccgg
     4261 agtcggtgca cgaagccgac agcccgcgtg cgccgcaggc ggtgttcgag ctgggcgtgc
     4321 cgctgttcgg catctgctac ggcatgcaga ccatggccga gcagctgggc ggcaaggtgc
     4381 agggctccga cctgcgcgag ttcggctacg cccgcgtcga cctggtcggc aagagccgcc
     4441 tgctcgacgg catcgaagac cacatggccg acgacggcat gctggcgctg gacgtgtgga
     4501 tgagccacgg cgacaaggtc accgcgctgc cggaaggctt ccacatcgtc gccagcaccc
     4561 cgagctgccc gatcgctgcc atggccgacg acagccgcgc ctactacggc gtgcagttcc
     4621 acccggaagt gacccacacc aagcagggcg gccgcatcct gtcgcgcttc gtgctggacc
     4681 tctgcggctg cgaagcgctg tggaccccgg ccaacatcgt cgacgacgcc atcgccaacg
     4741 tgcgcgccca ggtcggcgac tccaaggtgc tgctcggcct gtccggcggc gtcgactcct
     4801 cggtggtggc cgcgctgctg catcgcgcca tcggcgacca gctgacctgc gtgttcgtcg
     4861 acaacggcct gctgcgcctg cacgagggcg accaggtgat gaccatgttc gccgagaaca
     4921 tgggcgtcaa ggtgatccgc gccaatgccg aagacaagtt cctcggtcgc ctggccggca
     4981 tcagcgatcc ggaagagaag cgcaagatca tcggtcgcag cttcatcgag gtgttcgacg
     5041 aagaggccac caagctgcag ggcatcgact tcctcgccca gggcaccatc tacccggacg
     5101 tgatcgagtc ggccggcgcc aagaccggca aggccaaagt gatcaagtcg caccacaacg
     5161 tcggcggcct gccggaagac atgcagttca agctggtcga gccgctgcgc gagctgttca
     5221 aggacgaggt gcgcaagatc ggcctcgaac tcggcctgcc ctacgacatg gtctaccgcc
     5281 acccgttccc gggcccgggc ctgggcgtgc gcatcctcgg cgaggtgaag aaggagtacg
     5341 ccgacctgct gcgtcgtgcc gaccacatct tcatcgagga gctgcgcaac ttcgactggt
     5401 accacaagac cagccaggcc ttcgtggtgt tccagccggt gaagtcggtc ggcgtggtcg
     5461 gcgacggccg tcgttacgcc tgggtcgtcg ccctgcgcgc ggtggaaacc atcgacttca
     5521 tgaccgcacg ctgggcgcac ctgccctacg agctgctgga gaaggtctcc aaccgcatca
     5581 tcaacgagat cgaaggcatc tcccgcgtca cctacgacgt gtcgagcaag ccgccggcga
     5641 ccatcgagtg ggagtgatcc ctcggcttcg ttgaggcagt gaacacaagg gcagcctagg
     5701 ctgcccttgt gctgtccaga gggtgtgagg gcgagggcgc tggccatggc gactgtcgcg
     5761 cgtgccccgg ctgcgcagct ccttgcacag ccaaagcgcc atgcaaggct agaaaacgct
     5821 gcagaccccg ctacccgtgg cgttgcgatg accgcgcaag atcctgcgca gcagtgcgca
     5881 aggagttgcg ccgcttttgg tgacaggctt cacgccacgc cgcgcacgcc gatctaccta
     5941 tatcctaagt aattgatatt taaagaataa atatggctgg cacggctctt gtacccgtca
     6001 tggctcccgg acctccaccg gtccatgacc caggcaagga gagcactcat gccaacaccc
     6061 gcgtacctgt ccatcgaagg caccaagcaa ggtctgatca ccgcaggcac cttcaccgag
     6121 ggctcggtcg gcaacatctt ccaggaaggt catgaggacc agatcctcgt cgaggcattc
     6181 cagcatcagg tcatcattcc gcgtgacccg cagtccggtc agccgaccgg ccagcgcgtg
     6241 cacaagccgc tgatgatcac caaggtgttc gacaagtcct cgccgctgct gttcaacgcg
     6301 ctgacctccg gcgagcgcct gaccaagtgc cgcctggagt ggtatcgcac ctcggccacc
     6361 ggcacccagg agcactactt caccatcgaa ctgcacgacg ccatcatcgt cgacgtgcaa
     6421 tcgcgcatgc cgaactgcca ggacccgaac atggcgcact tcacccacct ggaagacgtc
     6481 tacttcacct accgcaagat cgtctggacc cacgaagtct ccggcacttc cggctccgac
     6541 gactggcgca cgccgatctc cgtgtaaggc gggagccgcg caccacccca tcggccaggt
     6601 ctcctggccg atgctttttc tgcacgggcg agaagcagcg gatgttcgct gtggtcaggc
     6661 gctggagggc tggctggcgt gggctgggga agggcggttg agccggtggc cggcgcggcg
     6721 gctttgccgc atgatgcctg tgcttcacgt tcattcgcgg tgttgctccc ggttgcggca
     6781 gttgtccctg tcgactgtcg caaccggggc gacggtctga cggtctgcct gggatttcct
     6841 gtgccaatgc ttccacgtcg ctctcctgtc ggattctccc cgctggcccg catgaaggcc
     6901 ctggccctcg gcctgctgct gctggcggcg ctgctgtacg ccctggccac ccacctgctg
     6961 gctctgcatc cggcctgggg ttatctggca gccttcgccg aggcggcgat ggttggcgcg
     7021 attgccgact ggttcgccgt cacggccctg ttccgccacc ccctggggct gccgattccg
     7081 cacacggcga tcattccccg caagcaggcc cgtctcggcc gcaacctggc cgacttcatc
     7141 tgcacccact ttctcgccac gccgcaggtg atggccaagc tggaggcgtt cgacgccgcc
     7201 ggccggctgg ccagctggtt gcgtcagccg gccaacgccg atgcggtcgg tcggcagctg
     7261 gtcggcgtgg cccgctatgg catcggcgcc ctgcgcgacg agcgggtacg ccagttcatc
     7321 cagcgcaccg ccaccgccaa gctgcgcgag gtggatctgg cctgcgtcgg cggccagttg
     7381 ctcgacatgc tgacccacca gcgccgccat caggcgatgc tcgacgaggt gctggctcgg
     7441 ctggacgagc tgctgcagga cgaggcggtg cagcagcgca tcgccgccgg cctgtccagc
     7501 gagttcagcg ccctgcgttt tcgcctgttc ggcaaggaca tcgggctgga cgagaaggct
     7561 gggcagtggt cggcggacaa ggtggtgcgc cgcgtctcgg ccctgatcgg cgagatcagc
     7621 cgcgacagcc agcacgagct gcgcggcaag ttcgatgcct gtgtcgccgt cctgatcggc
     7681 aagctcaagg acgatcccga cttccgcctc aagggcgagc aaatccgcga gcagctgctc
     7741 gctcatccgg cggtggccgg ctacctgcgc aagctgtggg acgatctggt ggactggctg
     7801 caggccgatc tggccgacgc cgactcgacc atccgccggc ggctgatccg cctctgccag
     7861 cgtctgggcg aggcgctggc ggcggacgag gcgatgcgcc gctggctcaa cgaccagctg
     7921 ctggaggtgg cgccaaccct ggtggaacgc caccgcgagg acatccgccg ctacatcgcc
     7981 gagcgggtgg agaagtggaa cagcgacgaa ctggtcgccc agctggagta caacgtcggc
     8041 aaggacctgc agttcatccg catcaacggc accctggtcg gcggtctggt cggcctggtg
     8101 ctgcatgccg tgacccgcct gctcgccagc tgatccgccg cgccgccctg acgaggcgac
     8161 ggctggcagc gggggagcgc tgttgtgtcg caggcggggc tcgcatcgag cgcagagaaa
     8221 atgagaaact ctgtcatctg cgtttgcggc ccgactacct gcggcggcgc gccatctttc
     8281 gcaagcttgg gggcccgatg tcctttaccc gcagacaagt cctcaccggc ctggctggcc
     8341 tcggcgtgct cggcctcgcc ggcggcggcg cgcgctactg gctggggcgt ccggccgcaa
     8401 cgactaccca cgactatgag ttgatcgccg cgccgctgga tgtggagctg gtgcccgggc
     8461 accagacccg cgcctgggcc tacggcggcc agctgcccgg gcaggaactg cgcgtgcgtc
     8521 agggcgattg gctgcgggtg cgcttcatca accggctcga ggtgccgagt accatccact
     8581 ggcacggcat ccgtctgccg ctggccatgg acggcgtgcc ctacgtgtcg cagctgccgg
     8641 tgctgcccgg cgaatacttc gactaccgct tccaggtgcc ggatgctggc agcttctggt
     8701 accacccgca caccgccagc ggcgagcagc tggggcgcgg gctggtcggt ccgctgatcg
     8761 tcgaggagcg tgagccgagc ggcttccgcc acgagcgcac gctgagcctg aagagctggc
     8821 atgtggacga gcagggcgcc ttcagcgact tcagcgtgcc gcgcgaagcc gcccgcggcg
     8881 gcaccgcggg gcggctgagc acggtcaacg gcgagcatgc gccgaccc
//
LOCUS       DATCUN010000058        17138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_115067, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000058 DATCUN010000000
VERSION     DATCUN010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 17138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17138
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115067"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VIO83_02115"
     CDS             complement(<1..297)
                     /locus_tag="VIO83_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY8330115.1"
                     /translation="MSKLRISALLFALLSGFSVAATAATAAEVPAAQPLAVQAPVVAP
                     LARVNLNSADAETLARELNGIGEAKARAIVEYREAKGAFTSVDELLEVKGIGAAT"
     gene            506..739
                     /locus_tag="VIO83_02120"
     CDS             506..739
                     /locus_tag="VIO83_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008097290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330116.1"
                     /translation="MQTPKDLLREQLAQAAEQFKSEQNGEVVIYAAQPAPEKRPWRKR
                     PSIHDEVFAKELDSMRKQKAETEQGDSSRAPSA"
     gene            complement(983..2131)
                     /locus_tag="VIO83_02125"
     CDS             complement(983..2131)
                     /locus_tag="VIO83_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HEY8330117.1"
                     /translation="MRTKRAFKYRFYPTPEQDALLARTFGCVRSVYNTILAWRTEAFQ
                     HEKTRIGYKDASAQLTALKKDPARAYLNEVSSVPLQQCLRHQQQAFTNFFEKRAGYPT
                     TKSKKHRQSAEFTASAFRFRDGQLFLAKCKEPLVIRWTKGRETLPSAPSTVTVSRDTA
                     GRYFVSMLCEVETQSLPVSPRTTGIDLGLKALFVTADGEQIDNPRHTAKYAARLAKAQ
                     RALSRKQRGSKNRAKARLKVARLHAKIADTRRDGLHKLTHRLIHENQVVCVESLAVKN
                     LIRNRSQSKAIADAGWGELVRQLEYKAAWAGRQLVKIDRWFPSSKRCSCCGHTLTSLP
                     LKARDWTCPACAAEHDRDLNAAINIKAAGLAVLALGENVSGIGRVPLS"
     gene            2270..3061
                     /gene="scpB"
                     /locus_tag="VIO83_02130"
     CDS             2270..3061
                     /gene="scpB"
                     /locus_tag="VIO83_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY8330118.1"
                     /translation="MNLADPRQLATVLEAILLAAGKPLPLERLAELFDEHERPSPKLL
                     REALGVLGSSCLGRGFELSEVASGYRLQVRERFSPWVGRLSEERPQRYSRALLETLAL
                     IAYRQPITRGEIEEVRGVAVNSNIVKTLLEREWIRVVGYREVPGRPAMFATTREFLDN
                     FNLKNLDELPPLAALRELEPRLPAEDLDPEVPAGLQARADLALAEGNEEASGEPRSET
                     NFRSLLAELDAMEQGLKSDFDDLPQLQLDDEDAAAQTAASAERAD"
     gene            3196..4302
                     /locus_tag="VIO83_02135"
     CDS             3196..4302
                     /locus_tag="VIO83_02135"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY8330119.1"
                     /translation="MNEEYSSAESAEEYRPAGEKLQKVLARMGVGSRRDVEEWIAQGR
                     IKVNGVDATLGQRIGYKDAIALDGRLLRREEDEVVRRVLIYNKPEGEVCTRSDPEGRP
                     TVFDRLPRPKSGRWINIGRLDINTTGLLMFTTDGELANRLMHPSYEMDREYAVRVRGE
                     VTDEMLENLKNGVVLEDGPARFTDVKEAPQGDGFNRWFHCVVMEGRNREVRRLWESQG
                     LVVSRLKRVRFGPVFLTADMSVGRWREMDQVEVDILAGEVGLRPMALPGMTGKARDKL
                     DRQQRKSAKPVAGERSRSSRGDSRDEGRGQGSQLAERPADVKRNQRPGKARDDRSSPN
                     LTERPSRKPAAAAQPAKRRSQPAGEGQRPGFRRK"
     gene            complement(4378..4695)
                     /gene="sugE"
                     /locus_tag="VIO83_02140"
     CDS             complement(4378..4695)
                     /gene="sugE"
                     /locus_tag="VIO83_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008365971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEY8330120.1"
                     /translation="MPWLILLLAGLLEVGWAIGLKYTDGFTRPLPTILTVAAMLASIA
                     LLGLAMKELPLGTAYAIWTGIGAVGTVLAGILLFGESLTPLRLCSVLLIVAGLVGLKL
                     AAS"
     gene            complement(4838..5758)
                     /gene="rdgC"
                     /locus_tag="VIO83_02145"
     CDS             complement(4838..5758)
                     /gene="rdgC"
                     /locus_tag="VIO83_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEY8330121.1"
                     /translation="MWFRNLLVYRLTQDLQLDAEKLEAALASKPARPCASQELATYGF
                     VAPLGKGADAPLVHASQDFLLIAARKEERILPGSVVRDALKEKIDEIEEQQMRKVYKK
                     ERDQLKDEIVQTFLPRAFIRRNMTFAAIAPQQGLILVDASSPKKAEDLLSTLREALGS
                     LPVRPLSVKVAPTATFTDWMKIQQASNGLQLLDECELRDTHEDGGIVRCKRQDLGSDE
                     VQNHLAAGKLVTKLSLAWSDKLSLLIDDKLALKRLRFEDLLQDQAEQDGGDDALAQQD
                     ASFTLMMLTFVEFLPQLIEALGGEEIPQGI"
     gene            5950..6025
                     /locus_tag="VIO83_02150"
     tRNA            5950..6025
                     /locus_tag="VIO83_02150"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5983..5985,aa:Val,seq:tac)
     gene            6041..6117
                     /locus_tag="VIO83_02155"
     tRNA            6041..6117
                     /locus_tag="VIO83_02155"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6075..6077,aa:Asp,seq:gtc)
     gene            6303..7010
                     /locus_tag="VIO83_02160"
     CDS             6303..7010
                     /locus_tag="VIO83_02160"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY8330122.1"
                     /translation="MKHHRLAAAVALVGLVLAGCDKQAAEKAQELKTPAEKASYGIGL
                     NMGRSLTQEGMNDLDSKAVALGIEDAMAKKEQRLTDEQLMEAFGFLQKRAEERMAALG
                     DENAKAGKKFLEENGKREGVVTTASGLQYEVLKKADGAQPKADDVVSVHYEGKLVDGT
                     VFDSSIKRGAPIDLPVGGVIPGWVEGLQLMHVGEKYKLYIPSELAYGAQSPSPMIPAN
                     SVLVFELELLSIQKKPE"
     gene            7508..7837
                     /locus_tag="VIO83_02165"
     CDS             7508..7837
                     /locus_tag="VIO83_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8330123.1"
                     /translation="MGVQVIECDGQPEYAVLPWAEYQELLAAAGRQPVARSAAPLVQE
                     PPVFNLANLRSLREARNLAPEQLARAVGISPHYLSLIESGERQPDQAIQRALTRLFAA
                     PPEGSSD"
     gene            7834..8202
                     /locus_tag="VIO83_02170"
     CDS             7834..8202
                     /locus_tag="VIO83_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330124.1"
                     /translation="MTLRISRQHWQGLLEALEEARCQRHLLTYRSLIERLQLPAPAMT
                     TLAAALEYLAALDAQAGQPLRSALVISQGASRLPRPGFFECAGRLGRFSGAPDGTAAA
                     AWHAAEVVRVFEFEFVGFSE"
     gene            8202..8675
                     /locus_tag="VIO83_02175"
     CDS             8202..8675
                     /locus_tag="VIO83_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEY8330125.1"
                     /translation="MQLFWRLRARMGYLLARRLFHWHWLVGQPRAWRWMEGQFARMAN
                     LGDARAQDFYGHLLLFRGQGLGAREEGLRLLRLAAAQGQVKAAFQLGLQALKGSAQQA
                     PNAAAALPYWRQAAAGGHPLAAHKLAELLREGAPGVEADPAEAERLAMRARELGL"
     gene            complement(8719..9447)
                     /locus_tag="VIO83_02180"
                     /pseudo
     CDS             complement(8719..9447)
                     /locus_tag="VIO83_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003223117.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(9567..11648)
                     /locus_tag="VIO83_02185"
     CDS             complement(9567..11648)
                     /locus_tag="VIO83_02185"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HEY8330126.1"
                     /translation="MKRSLSCSALALVLTTSVLPLTNAAARTNPWDGLQPDREQTVAS
                     LNVVELLKRHHYNKPPLNDARSSQIFDDYLKSLDPARSYFTAADIASFEPWRYRFDDY
                     LKSGELQPGFVIYKRQLERMQERLEFALSLLDQGVDKFDFSRDESLQTDREKAPWAAD
                     NKALDELWRKRVKDEVLRLKLAGKEPKAIQELLSKRYKNQLARLAQTRSEDIFQAYIN
                     AFTQTYDPHTNYLSPENAENFDINMSLSLEGIGAVLQSDNEHVKVVRLVPAGPADKSK
                     QLKPADRIIGVAQGDKEMVDVVGWRLDEVVKLIRGPKDSVVRLEVIPASNAANDQTSK
                     VVPITREAVKLEEQAAKKSVLKLEHDGHDYKLGVIEIPAFYLDFKAYRGGDPNYKSTT
                     RDVRKLLSELQQEKVDGVVIDLRNNGGGSLQEATELTGLFIDQGPTVLVRNSDGRVDV
                     LADEDQGTYYKGPMAVLVNRLSASASEIFAGAMQDYHRALILGGQTFGKGTVQTIQPL
                     NHGELKLTLAKFYRVSGQSTQHQGVLPDIAYPAIVDVKEIGESALPAAMPWDRIAPAI
                     QPAGDPFRPFLAQLKALHEARTAKNPDFEFTRKSLELSQQLMQNTEVSLNEDKRKAQR
                     NDIEGRQLALENARRSAKGEAPLKELKDEEELADASEEDKLPPEKDAFLAESGRILLD
                     YLTLNSAVAKH"
     gene            11830..12792
                     /locus_tag="VIO83_02190"
     CDS             11830..12792
                     /locus_tag="VIO83_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEY8330127.1"
                     /translation="MNALQGIEGQLEWGAQPRPECDVGQIRIRVAAAGLNRADLLQRN
                     GLYPPPPGVTPVLGLECSGVVSEVGAGSRWRVGDRVCALLAGGGMAEEVVVDAGHALP
                     VPEGMSLQEAAVLPEVYATAWLNLFQLGRLLPGEKVLLHAGASGVGSAGIQLCKAFGS
                     PCWVSVGSAERLAYCEALGAQGGVIRGESLDGLRDFAPFDVILDPVGASYAELNLKLL
                     GQDGRWVIIGLMGGRKAQLDLALLLGKRIQMIGSTLRNRDAESKAQLLAELEQHVWPL
                     FGEGRLKPQLERSYPISDAEAAFAALASNEVQGKVVLVVDASLS"
     gene            complement(12845..13498)
                     /locus_tag="VIO83_02195"
     CDS             complement(12845..13498)
                     /locus_tag="VIO83_02195"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8330128.1"
                     /translation="MALAIFDLDDTLIGGNSPSLWLEHLADIGWLDRESFIPREQELV
                     ALYAALQVSLEDYMAFCLQPLVGRSREEVEYVAGPFVEEFIEPLIHSDGMRAIANHRA
                     AGDRILVISASPTFLVQAIAARLGIAEVLATELELQHGHYSGRISGIPAAREGKIQRL
                     AQWLEAQGEVLSGAHFYSDSRNDLPLLCHVEHPHAVNPDAALREHAERQGWEILHWR"
     gene            complement(13513..14802)
                     /locus_tag="VIO83_02200"
     CDS             complement(13513..14802)
                     /locus_tag="VIO83_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HEY8330129.1"
                     /translation="MREALNQGLIDFLKASPTPFHATASLAARLEAAGYQRLDEREPW
                     QAQPGGRYYVTRNDSSLIALQLGRRAPLEGGLRLVGTHTDSPCLRVKPQPELARQGYW
                     QLGVEVYGGMLLAPWFDRDLSLAGRVTFKHDGKLHSRLIDFKAPIAVIPNLAIHLNRE
                     ANQGWEINKQTELPPILAQLATGEQRDFRTLVAKQLEHEHGIQVEAVLDFELSFYDTQ
                     SAAVIGLEQEFIAGARLDNLLSCYAGLEALLAAGDQESCVLVCTDHEEIGSCSACGAD
                     GPLLEQVLRRLLGDEETFVRCIQRSLLVSADNAHGVHPNYADKHDGNHGPSLNGGPVI
                     KVNNNQRYASNSETAGFFRHLCLEHAVPVQCFVSRSDLGCGSTIGPITASKLGVPTID
                     IGLPTFAMHSIRELAGSQDLAHLVKVLTAFYQSPELA"
     gene            complement(14862..15497)
                     /locus_tag="VIO83_02205"
     CDS             complement(14862..15497)
                     /locus_tag="VIO83_02205"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY8330130.1"
                     /translation="MPLSNIQIVHQDAALLVINKPTLLLSVPGRAEDNRDCLVTRLQD
                     NGYPEARIVHRLDWETSGLIVLARDADSHRELSRQFHDRETEKAYTALCWGQPATDSG
                     RIELPLRYDPPNKPRHIVDHELGKHALTFWRIVERCGDWCRVELTPITGRSHQLRVHM
                     LAIGHPLLGDRLYAHEQALAAHERLCLHASLLCLTHPQTGERMRFESPAPF"
     gene            complement(15561..15815)
                     /gene="minE"
                     /locus_tag="VIO83_02210"
     CDS             complement(15561..15815)
                     /gene="minE"
                     /locus_tag="VIO83_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEY8330131.1"
                     /translation="MSIFDFLLSRKKTTSASIAKERLQIIVAHERGSRGQPDYLPALQ
                     EELIAVIRKYVPIDQDQVQVALENQGTCSILELNITLPDR"
     gene            complement(15812..16627)
                     /gene="minD"
                     /locus_tag="VIO83_02215"
     CDS             complement(15812..16627)
                     /gene="minD"
                     /locus_tag="VIO83_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEY8330132.1"
                     /translation="MAKIIVVTSGKGGVGKTTSSAAIGTGLALRGHKTVIVDFDVGLR
                     NLDLIMGCERRVVYDFINVIHNEATLSQALIKDKRLDNLYVLAASQTRDKDALNKEGV
                     ERVIEELKKDFEYILCDSPAGIETGAHLAMYFADIAIVVTNPEVSSVRDSDRILGLLA
                     SKSQRAEKGEEPIKEHLLLTRYNPERVARGEMLSVDDVEEILSIGLIGVIPESQAVLK
                     ASNQGVPVILDEQSDAGQAYGDAVDRLLGKEVPHRFLEVQKKGLLQRLFGGRE"
     gene            complement(16682..>17138)
                     /gene="minC"
                     /locus_tag="VIO83_02220"
     CDS             complement(16682..>17138)
                     /gene="minC"
                     /locus_tag="VIO83_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEY8330133.1"
                     /translation="GLPLLPPASGRERPLAEPVEPATPEPALAAPAEAAQPAEPEVKP
                     TLVITRPVRGGQQIYAHGGDLVVLSAVSPGAELIADGNIHVYGPLRGRVLAGAKGNAE
                     ARIFCQQLTAELLSIAGRYKVAEDLRRSPQWGKAVQVSLSGDVLNITRL"
BASE COUNT         2820 a         5295 c         5918 g         3105 t
ORIGIN      
        1 cgtcgccgca ccgatgccct tcacctccag cagttcatcc accgaagtga aggccccctt
       61 ggcttcgcga tattcgacaa tggcacgcgc cttggcctcg ccgataccat tcagctcgcg
      121 ggccagggtt tcggcatcgg cgctattcag attgactctg gccagcggcg caacgacggg
      181 ggcctgcaca gccagcggct gggcggcagg cacttcggcg gcagtggcgg cagtggcggc
      241 aacagaaaag cccgagagca gagcgaacaa cagagcggaa atacgcaatt tcgacatgat
      301 cgaccatccc tgtgagagta ggaaaatgcc gccttatccc gaggcagccc agcgaaccta
      361 aatcacgcca gcgaaatagc caatagtccc catttcgatt ggtaaaaggt ttattgcgca
      421 aaagcgaccc aattcacatt tgcgaccggc aagcgcggct ggcaaaattt gctccaccct
      481 actcacaaag acccacccca gatatatgca gacccccaaa gacctgttgc gcgaacaact
      541 ggctcaggcc gccgaacagt tcaagagcga gcagaacggc gaagtcgtta tctacgccgc
      601 ccagccagcg ccggaaaagc gcccgtggcg caagaggccg agcatccacg acgaagtatt
      661 cgccaaggaa ctcgacagca tgcgcaagca aaaggccgag acggaacagg gcgacagcag
      721 ccgcgccccg agcgcgtgaa acacgccaag agtcctacct gccgtgcccc tgtaatctaa
      781 tagcgcctgc tgcccagcag gcctgcgcgt ctcgcccaac gcccatggtc tagagttctt
      841 tctgcgcccc gcgtaatcgc cgcaccgctg cggcggcggg gcgcgctgga cgtgatcccc
      901 accccgcacg cattccgatc agcagggcca gcgcattgac tgctctccgc cctgaaggac
      961 ggagattccc aattcatcga gatcaggaca gcggtacacg accgatgcca cttacgttct
     1021 ctccaagggc taacaccgcc agcccggcgg ctttaatgtt gatcgcggcg ttcaggtcgc
     1081 gatcgtgttc cgcggcgcag gccggacagg tccagtcgcg ggccttcagc ggcaacgagg
     1141 tcagggtgtg accgcagcac gagcagcgtt tgctgctggg gaaccagcgg tcaatcttga
     1201 ccagctggcg cccggcccac gccgccttgt actcgagctg gcgcaccagc tcgccccagc
     1261 cggcatcggc aatggccttg ctctggctgc ggttgcggat caggttcttc accgccaacg
     1321 actcgacaca gaccacttgg ttttcgtgaa tcagtctgtg cgtcagcttg tgcaagccgt
     1381 cgcgtcgggt gtcagcgatt ttcgcgtgca gtcgagcaac cttcagccgg gccttggccc
     1441 ggttcttcga tccccgctgt ttgcggctca acgcccgctg ggctttcgcc aaccgggcgg
     1501 cgtacttcgc ggtatgccgt gggttgtcga tctgttcgcc gtcggcggtg acgaacagcg
     1561 ccttcaggcc aaggtcgatg ccggtcgtcc gcggcgacac cggcagcgac tgggtctcga
     1621 cctcgcacag catcgaaacg aaataccggc ccgcggtgtc gcgggaaacc gtcactgtcg
     1681 acggcgccga gggcaatgtt tcacgcccct tcgtccagcg gatcaccaac ggttccttgc
     1741 acttggcgag gaacagctgg ccgtcgcgaa atcggaaggc cgaggctgtg aattcggccg
     1801 actggcggtg ttttttcgac ttcgtcgtcg ggtagccggc gcgtttctca aagaagttcg
     1861 tgaacgcctg ttgctggtgc cggaggcact gctgcagggg aacgctggac acctcgttga
     1921 gataggcccg cgccgggtct ttcttcaatg cggtcagctg ggcactggcg tccttgtagc
     1981 caatgcgcgt tttctcgtgc tgaaacgcct cggtacgcca ggccaggatc gtgttgtaga
     2041 cggaacgcac gcagccgaac gtccgcgcca gcagggcatc ctgctcgggc gtcgggtaga
     2101 aacggtactt gaacgcacgc ttcgtcctca tacgtataac tgtatgcata tccagcaaag
     2161 cgatcaaccc aagatagccc aatccaagga gaagcgagaa caggggcacc ctgcgggcgc
     2221 cacgctatcc ctccccggca tgaatgccga ggtttctcgc gcaaatctga tgaatcttgc
     2281 cgatccgcgc cagttggcga cggtgctcga ggcgatcctg ctggcggccg gcaagccgct
     2341 gccgctggag cgcctggccg agctgttcga cgagcatgag cggccctcgc ccaagctgct
     2401 gcgcgaggcc ctcggggtgc tgggcagctc ctgcctgggg cgtggcttcg agctgagcga
     2461 ggtggcctcc ggctaccgcc tgcaggtgcg cgagcgcttc tcgccctggg tcggccggct
     2521 gtcggaggag cgtccgcagc gctactcgcg cgcgctgctg gagaccctgg cgctgatcgc
     2581 ctaccgccag ccgatcaccc gtggcgagat cgaggaggtg cgcggtgtgg cggtcaacag
     2641 caacatcgtc aagaccctgc tcgagcgtga gtggatccgc gtggtcggct accgcgaggt
     2701 gccgggtcgg ccggcgatgt tcgccaccac ccgggagttc ctcgacaact tcaacctgaa
     2761 gaatctcgac gagctgccgc cgctggccgc actgcgcgag ctggagccgc ggctgccggc
     2821 cgaggatctc gatcccgagg tgccggccgg tctgcaggcg cgggccgatc tggccctggc
     2881 cgaaggcaac gaggaggcct ctggcgagcc gcgcagcgaa accaacttcc gcagcctgct
     2941 ggccgagctg gatgcgatgg agcagggctt gaagagtgat ttcgacgacc tgccgcagct
     3001 gcagctggac gatgaagacg ctgccgcgca gaccgctgcc agcgctgagc gggccgactg
     3061 acgcgctaca gccgtaccgc aggtccggtc tgtcgcctgc ggggtgcgcg ttttcggtca
     3121 tcggcgtatg atgcgtgccc gctcttttag atagagccag ttcacagaat ccggttacac
     3181 cgggaggtgc ccagaatgaa cgaagaatac agttccgccg agtccgccga agagtaccgc
     3241 cctgctggcg aaaagctgca gaaggtgctg gcgcgcatgg gcgtcggctc gcgccgtgac
     3301 gtcgaggagt ggatcgccca gggccgcatc aaggtcaacg gcgtcgacgc caccctcggt
     3361 cagcgcatcg gctacaagga tgccatcgcc ctcgacggcc gcctgctgcg tcgcgaggaa
     3421 gacgaagtgg tacgccgcgt gctgatctac aacaagccgg aaggcgaggt ctgcacccgt
     3481 tccgatccgg aaggccggcc gaccgtgttc gaccgcctgc cgcggccgaa gagcggtcgc
     3541 tggatcaaca ttggccgtct cgacatcaac accaccggcc tgctgatgtt caccaccgac
     3601 ggcgagctgg ccaaccgcct gatgcacccg tcctacgaga tggaccgcga atacgccgtg
     3661 cgcgtgcgcg gcgaagtcac cgacgagatg ctcgagaacc tgaagaacgg cgtggtgctc
     3721 gaggatggcc cggcgcgctt caccgacgtc aaggaagccc cccagggcga cggcttcaac
     3781 cgctggttcc attgcgtggt gatggaaggc cgcaaccgcg aggtgcgccg cctgtgggag
     3841 tcccaggggc tggtggtcag ccgtctgaag cgcgtgcgct tcggtccggt gttcctcacc
     3901 gccgacatgt cggtaggccg ctggcgcgaa atggaccagg tcgaggtgga tatcctcgcc
     3961 ggcgaagtcg gcctcaggcc gatggccctg ccggggatga ccggcaaggc gcgcgacaag
     4021 ctcgatcgcc agcagcgcaa gtcggccaag ccggtagcgg gtgagcgcag ccgttcgtcg
     4081 cgcggcgaca gccgggatga gggccgcggc cagggttcgc agctggccga gcgtccggcc
     4141 gacgtcaagc gtaaccagcg tccgggcaag gcgcgtgacg accgttcctc gccgaacctg
     4201 accgagcgcc cgtcgcgcaa gccggccgcc gcagcgcagc cggccaagcg tcgttcgcag
     4261 ccggcgggtg aaggccagcg tccgggcttt cgccggaagt aagccgccgc cagtcggcgt
     4321 ggctgaatga agaaggcgac ccgtgggtcg ccttcttttt gtctgtgatt ttccgagtca
     4381 gctggcggcc agcttcagac cgaccaggcc ggcgacgatc agcagcacgc tgcacagccg
     4441 cagcggggtc agtgattcgc cgaacagcag gatcccggcg agcacggtgc cgaccgcacc
     4501 gatgccggtc cagatcgcat aggcggtgcc cagcggcagc tctttcatcg ccaggccgag
     4561 cagggcgatg ctggcgagca tggcggcaac ggtcaggatg gtgggcagcg ggcgagtgaa
     4621 gccgtcggtg tatttcaggc cgatggccca gccgacctca agcaggccgg cgagcagcag
     4681 gataagccaa ggcatgggaa acctcctcca gtggcaagga aggggccgtc cccgggtggt
     4741 cactcggcaa gtgctgaggt cgtcctcacg tgcagcgcat tatccatgtg cacccgtttg
     4801 gggcaagcga cggtcgtcgc cggccccgtg gcggaactca gatcccctgc gggatttctt
     4861 cgccgccgag ggcctcgatc agctgcggca ggaactcgac gaaggtcagc atcatcaggg
     4921 tgaagctggc gtcctgctgg gccagggcat cgtcgccgcc atcctgttcg gcctgatcct
     4981 gcagcaggtc ctcgaagcgc aggcgcttga gggccagctt gtcgtcgatc agcagggaca
     5041 gcttgtccga ccaggccagg ctcagcttgg tcaccagctt gccggcggcc aggtggttct
     5101 gtacttcgtc gctgccgaga tcctggcgct tgcagcgcac gatgccgccg tcctcgtggg
     5161 tgtcgcgcag ctcgcactcg tcgagcagtt gcaggccgtt gctggcctgc tggatcttca
     5221 tccagtcggt gaaggtggcg gtgggggcga ccttcacgct cagcgggcgc acgggcaggc
     5281 tgcccagggc ttcgcgcagg gtggaaagca ggtcttcggc cttcttcggg ctggaggcgt
     5341 cgaccaggat caggccctgc tggggggcaa tggcggcgaa ggtcatgttg cggcggatga
     5401 aggcgcgcgg caggaaggtc tgcacgatct cgtccttgag ctggtcacgc tccttcttgt
     5461 agaccttgcg catctgctgc tcttcgatct cgtcgatctt ctccttcagc gcgtcgcgca
     5521 ccacgctgcc gggcagaatg cgttcctcct tgcgcgcggc gatcagcagg aagtcctggc
     5581 tggcgtgtac cagcggcgca tcggcaccct tgcccagggg ggctacgaag ccgtaggtgg
     5641 ccagctcctg gctggcgcag gggcgggcgg gtttgctggc cagggccgcc tcgagttttt
     5701 cggcgtccag ttgcagatcc tgggtaaggc ggtagacaag caggttgcgg aaccacatgg
     5761 cgacgaggca ctccatcaag caaagaaggg cattattcct gctggcgcgc tcgccggcca
     5821 ccggcgattg tgccaagtgg ttggtttgcc tcgaattttt tttgaaaaaa acgcttgcca
     5881 aggtgaagcg acctgcctag aatgcgcacc acttcgagag caaacgctaa caagcgaatg
     5941 caactgaatg ggtgattagc tcagctggga gagcatctgc cttacaagca gagggtcggc
     6001 ggttcgatcc cgtcatcacc caccactctc gaggttacgc gcagcggtag ttcagtcggt
     6061 tagaataccg gcctgtcacg ccgggggtcg cgggttcgag tcccgtccgc tgcgccattt
     6121 ttctccccaa agtgacaatt caagtcaaat atccccaaaa gttcaacgcg gagcatcgcg
     6181 atttgaactt ttcgtcgttt ctgcattcca aaaaattcca gccataggca gcaacgccgt
     6241 cagcctgcta agctcgcgtt ttccgagtat gccgctgtgg cacctatcat taaggaatat
     6301 gcatgaaaca tcatcggttg gccgctgcgg tggctctggt cggcctggtc ctcgctggct
     6361 gtgacaagca ggcggcagag aaggcgcagg agctcaagac cccggcagag aaagcctcct
     6421 acggtatcgg cctgaacatg ggtcgcagcc tgacccagga aggcatgaac gatctcgact
     6481 ccaaggcggt cgccctcggc atcgaggatg ccatggcgaa gaaagagcag cgtctgaccg
     6541 acgaacagct gatggaagcc ttcggtttcc tgcagaagcg tgccgaggag cgcatggcgg
     6601 cgctcggcga cgagaacgcc aaggctggca agaagttcct cgaggaaaat ggcaagcgcg
     6661 aaggcgtggt gaccaccgct tccggtctgc agtatgaagt cctcaagaag gccgacggtg
     6721 cgcagcccaa ggccgacgac gtggtcagcg tgcactacga gggcaagctg gtcgatggca
     6781 cggtgttcga cagctcgatc aagcgtggcg ctcccatcga cctgccggtc ggcggcgtga
     6841 ttccgggttg ggtcgaaggc ctgcagctga tgcatgtcgg cgagaagtac aagctgtaca
     6901 tccccagcga gctggcctac ggcgcgcaga gcccgagccc gatgattccg gccaactcgg
     6961 tgctggtgtt cgagctggag ctgctgagca tccagaaaaa gcccgaataa gctggtagcc
     7021 ggttttccca ccaacgcccc gcttgtcggg gcgttggtgt ttctacgtgt gcggtccttg
     7081 acggtcggag tggaaaaccc gtcgggccgg gtgggtgtgc tgtggttata cacatctgga
     7141 tatgcactcg gcagtgctgt caagagctgc atcgggcctg tcatgaataa ttttaacttt
     7201 ctgatttgta aagggttttt tgtttgaaca aaaaatgccc gatctgtctg gaggccgcat
     7261 tccatgggcg tcctgtcgac tcttccagat attgcccaca aggttatcca caggatttgt
     7321 gggtaacgcc cgcaagccca gtaaccatgc gccttggctg ggtgtgggga taagtgacag
     7381 ggtaaaacgc accgccgctg cgacaaaaag tcctgccgcg gggcgcttgc cggcagccct
     7441 gatcgcggtg ctgccatggc agttcgcgca ggcctgttaa actgaaacaa aagggagtga
     7501 tgacctcatg ggtgtacaag tgatcgagtg cgacggccag ccggaatatg cggtgctgcc
     7561 gtgggccgaa tatcaggagc tgctggctgc cgccgggcgc cagcctgtcg cgcgttcggc
     7621 agcgccgttg gtgcaggagc cgccggtctt caatctggcc aacctgcgca gcctgcgcga
     7681 ggcgcgcaat cttgccccgg agcagctggc gcgcgcagtg ggcatcagcc cgcattacct
     7741 gagcctgatc gaaagcggtg agcgccagcc agatcaggcc atccagcgcg cgctgacgcg
     7801 gctgttcgcg gcgccacccg aaggatcttc ggattgaccc tgcgcatcag tcggcagcac
     7861 tggcagggcc tcctggaggc cttggaggag gcgcgttgcc agcgtcacct gctgacctat
     7921 cgctcgctga tcgagcgtct ccagctgccg gcgccggcga tgaccaccct ggctgcggca
     7981 ctcgagtatc tggcggcact ggacgcgcag gcggggcagc cactgcgcag tgcgttggtg
     8041 atcagccagg gtgccagccg tttgccgcgc ccgggattct tcgagtgcgc ggggcgcctc
     8101 ggccgcttca gtggcgcgcc ggatggcacg gctgccgcag cctggcatgc cgccgaggtg
     8161 gtgcgggtct tcgaattcga gtttgtgggg ttctccgagt gatgcagctg ttctggcgcc
     8221 tgcgggcgcg catggggtat ctgctggccc gtcgcctgtt ccactggcat tggctggtgg
     8281 gtcagccgcg tgcctggcgc tggatggagg ggcagttcgc gcgcatggcc aacctcggcg
     8341 atgcccgggc ccaggatttc tacggtcatc tgctgctgtt ccgcgggcag ggcctcggag
     8401 ctcgcgagga aggtctgcgc ctgctgcgtc tggctgcggc ccaggggcag gtcaaggcgg
     8461 ccttccagct tggcctgcag gccctcaagg gcagtgccca gcaggcgccg aatgccgccg
     8521 cggcgctgcc ctactggcgg caggcggcag ccggcgggca tccgctggct gcgcacaagt
     8581 tggccgaatt actgcgcgaa ggggcgcccg gcgtggaggc tgatccggct gaagccgagc
     8641 gcctggcgat gcgggcgcgc gagttgggcc tgtgacgggt caggcgcaga cctcgaggtc
     8701 gcgcagcttt tcgctggcgg gcttttcctg cgggcggccg agcaggtagc cctgcaccag
     8761 gtccacgccc atcccggcca gtacctgcaa ctcctctagc tgttccacgc cttcggcgat
     8821 cactcgggcg cgggcggttt gggccatgcg caggatggag ccgacgaact cgcgcttgag
     8881 cgggtcgagg tggatgccgt cgatgaagtg gcggtcgatc ttgacgatat ccggccgcag
     8941 ctccgaccac aggcgtaggc tcgagtagcc ggcgccaagg tcgtccaggg cgatggagaa
     9001 gcccatggcc cggtagtgac gcagggcgcg ctggagcagg tcgaagtcct cgatcggggt
     9061 ctgctcggtc aattcgatca ccacccgcgc cggctcgatg ccgcagtcct gcagcaactg
     9121 cagcgtgcgc ccggactggt gccccggctc gaccagcagc tccgggcaga cattcaggta
     9181 gagcgtgccg gggaagcgct ggcggctgaa ctgggcgcag gcgcgggccc ggctggccat
     9241 ttccagctcg ttcagccgtc cggcattgcg ggccagggaa aacagcgtca gcggcgcatg
     9301 cagcgggctg ttggaggggc cgcgggtgag ggcttcgtaa ccgtggatgc gcttgctggc
     9361 gagcgagacg atgggctgga acagggtgtt gagcaggttg tggccgagga ttcgctccag
     9421 ttcgctcaac tgttcggtgg tggtcatgca gggctcggca gtcaatgaaa aaaacgcccc
     9481 ggacagctgc cggggcgggg tgatttcacg actaaattat gacgatttga tgtcggcagg
     9541 tggtcgggcg accacctgcc gtcgcctcaa tgtttggcga ccgccgaatt gagggtgagg
     9601 tagtccagca ggatgcgtcc cgattcggcg aggaaagcgt ccttttccgg cggcagcttg
     9661 tcttcctcgc tggcgtcagc cagctcttcc tcgtccttga gttccttcag cggcgcctcg
     9721 cccttggcgc tgcggcgtgc gttctccagg gccagctggc gtccttcgat gtcgttgcgc
     9781 tgcgccttgc gcttgtcctc gttgaggctg acctcggtgt tctgcatcag ctgctggctc
     9841 agctccaggc tcttgcgggt gaactcgaag tccgggttct tggccgtgcg cgcctcgtgc
     9901 agggccttga gttgggcgag gaacgggcgg aacgggtcgc cggcaggctg gatcgccggg
     9961 gcgatgcggt cccacggcat ggcggccggc agggcgcttt cgccgatctc cttgacgtcg
    10021 acgattgccg ggtaggcgat gtccggcagc acaccctggt gctgggtgct ctgcccggag
    10081 acccggtaga acttggccag ggtcagcttt agctcgccat ggttgagcgg ctggatggtc
    10141 tgcacggtgc ccttgccgaa ggtctggccg ccgaggatca gggcgcggtg atagtcctgc
    10201 atggcgccgg cgaagatttc cgaggccgag gccgacaggc gattgaccag tactgccatc
    10261 ggccccttgt agtaggtgcc ctggtcctcg tcggccagca cgtcgacgcg gccgtcgctg
    10321 ttgcgtacca gcacggtcgg cccctggtcg atgaacaggc cggtcagctc ggtggcctcc
    10381 tgcagcgagc cacccccgtt gttgcgcagg tcgatgacca caccatcgac cttctcctgc
    10441 tggagttcgc tgagcagctt gcgcacgtcg cgggtggtac tcttgtagtt cgggtcgccg
    10501 ccgcggtagg ccttgaagtc caggtagaag gccgggatct cgatcacccc gagcttgtag
    10561 tcgtggccgt cgtgttcgag cttgagcacc gacttcttcg ccgcctgctc ttccagcttg
    10621 accgcttcgc gggtgatcgg taccaccttg ctggtctggt cgttggccgc attgctggcc
    10681 gggatcactt ccaggcgcac caccgaatct ttcgggccgc ggatcagctt gaccacttcg
    10741 tccaggcgcc agccgaccac gtcgaccatt tccttgtcgc cctgggcgac gccgatgatg
    10801 cggtcggccg gtttgagctg cttgctcttg tccgccggtc cggcgggcac caggcgcacg
    10861 accttgacgt gctcgttgtc gctctgcaac accgcgccga tgccttccag cgacaggctc
    10921 atgttgatgt cgaagttttc cgcgttttcc ggcgacaggt agttggtgtg cgggtcgtag
    10981 gtctgggtga aggcgttgat gtaggcctgg aaaatgtcct cgctgcgcgt ctgcgccagg
    11041 cgcgcgagct ggttcttgta gcgcttgctc agcagctcct ggatcgcctt gggctccttg
    11101 ccggccagct tcaggcgcag cacctcatcc ttgacccgct tgcgccacaa ttcgtcgagc
    11161 gccttgttgt cggcggccca gggggctttc tcgcggtcgg tttgcaggct ttcgtcgcgg
    11221 ctgaaatcga acttgtcgac gccctggtcg agcagcgaca gggcgaactc cagtcgctcc
    11281 tgcatgcgct cgagctgacg cttgtagatg acgaagccgg gctgcagctc gccgcttttc
    11341 aggtagtcgt cgaagcggta acgccagggt tcgaaggagg cgatgtcggc ggcggtgaaa
    11401 tagctgcgcg ccgggtcgag gctcttcagg tagtcatcga agatctgcga cgaacgggca
    11461 tcgttcagcg gcggcttgtt gtagtgatgg cgcttgagca gctccaccac gttgagactg
    11521 gcgacagtct gctcgcggtc gggctgcagg ccatcccagg gattggtgcg tgcagccgca
    11581 ttggtcagcg gcagaacgct ggtggtcagg acaagagcga gggcggagca ggacagtgag
    11641 cgtttcatca gattttcgcg tcgcagggag tcacgccaat taggcaacat aaaagacaca
    11701 tggttccatg gtgccagtat agggcgtcgg aggagggatg ctgacgtgaa gccagtttcc
    11761 gggtaacaat ccggccgcag actccggcgc gctcaacggg caccgggtat tcaacaatat
    11821 ggaggtagcg tgaacgcatt gcaaggtatc gaggggcagc tggagtgggg cgcgcagccg
    11881 cgcccggaat gcgatgtcgg gcagatccgc attcgcgtgg cggccgccgg tctcaaccga
    11941 gccgacctgc tgcagcgcaa cggcctgtac ccgccgccgc cgggcgtgac tccggtgctg
    12001 ggtctggagt gctccggggt ggtcagcgag gtgggtgccg gcagtcgctg gcgggtgggc
    12061 gaccgggtgt gcgcgctgct ggccggcggt ggcatggccg aggaggtggt ggtggacgcc
    12121 ggccacgccc tgccggtgcc cgaggggatg agcctgcagg aggccgcggt gctgccggag
    12181 gtctacgcca ccgcgtggct caacctgttc cagctgggcc gcctgctgcc gggcgagaag
    12241 gtgctgctgc atgccggcgc cagcggtgtc ggttccgccg gcatccagct gtgcaaggcg
    12301 tttggcagcc cgtgctgggt cagcgtcggc tcggccgaac ggctggccta ttgcgaggcc
    12361 ctgggcgccc agggtggggt gattcgcggc gagagcctcg acggcctgcg tgatttcgcg
    12421 ccgttcgacg tgatcctcga tccggtgggc gccagctacg ccgagctgaa cctcaagctg
    12481 ctcggccagg acgggcgctg ggtgatcatc ggtctgatgg gcgggcgcaa ggctcagctg
    12541 gatctggccc tgctgctggg caagcgcatc cagatgatcg gctcgaccct gcgcaaccgc
    12601 gacgccgagt ccaaggcgca gctgctggcc gagctggagc agcacgtctg gccgctgttc
    12661 ggcgagggtc ggctgaagcc gcagctggag cgcagctacc cgatcagcga tgccgaggcg
    12721 gccttcgccg cactggccag caacgaggtg cagggcaagg tggtgctggt ggtggatgcc
    12781 agcctgagct gagtggttcc gaggctgggg acgcgatgcg tccccggtcg ccgatggcgg
    12841 ccgctcagcg ccagtgcagg atctcccagc cctgtcgctc ggcatgctcg cgcagcgcgg
    12901 catcagggtt gaccgcatgg ggatgttcga cgtggcagag cagcggcagg tcgttgcgcg
    12961 agtcggagta gaagtgcgcc ccgctcagca cctccccctg cgcctccagc cactgcgcga
    13021 ggcgctggat cttgccctcg cgggccgcgg ggatgccgct gatgcggccg ctgtagtgac
    13081 cgtgctgcag ttccagctcg gtggccagta cctcggcgat gcccaggcgt gcggcgatgg
    13141 cctgcaccag gaaggtcggc gaggcggaga tcaccaggat gcggtcgcca gcggcgcgat
    13201 ggttggcgat tgcgcgcatg ccgtcgctgt gaatcagcgg ctcgatgaac tcctcgacga
    13261 aggggccggc gacgtactcg acctcttcgc gcgaacggcc gaccagcggc tgcaggcaga
    13321 aggccatgta gtcctccagg ctgacctgca gcgcggcata cagcgccacc agctcctgct
    13381 cgcggggaat gaaggactcg cggtcgagcc agccgatgtc ggccaggtgt tccagccaca
    13441 ggctggggct gttgccgccg atcagggtgt cgtcgaggtc gaagatcgcc aatgccacgc
    13501 tatgtgctcc gctcaggcca gttcggggct ctggtagaag gcggtcagca ccttcaccag
    13561 gtgggccagg tcctgactgc cggccagctc gcggatcgag tgcatggcga aggtcggcag
    13621 gccgatgtcg atggtgggca cgccgagctt gctggcggtg atcggcccga tggtcgagcc
    13681 gcagccgagg tcgctgcgcg acacgaagca ctgtaccggc accgcatgct ccaggcacag
    13741 atggcggaag aagccggcgg tttcgctgtt gctggcgtag cgctggttgt tgttgacctt
    13801 gatcaccggg ccgccgttga ggctcgggcc gtggttgcca tcgtgcttgt cggcatagtt
    13861 cggatgcacg ccgtgcgcat tgtcggcgga gaccagcagg gagcgctgga tgcagcggac
    13921 gaaggtttcc tcgtcgccaa gcaggcggcg cagcacctgc tccagcagcg ggccatcggc
    13981 gccgcaggcc gagcaggagc cgatttcctc gtggtcggtg cacaccagca cgcagctttc
    14041 ctggtcgccg gcggccagca gtgcctcgag gccggcatag caggacagca ggttgtccag
    14101 ccgcgcgccg gcgatgaatt cctgttccag gccgatcact gcggcactct gcgtatcgta
    14161 gaagctcagc tcgaagtcga gcacggcctc cacctggatg ccgtgttcat gctcgagttg
    14221 cttggcgacc agggtgcgga aatcgcgctg ctcgcccgtc gccagttggg cgaggatggg
    14281 cggcagttcg gtctgcttgt tgatttccca gccctggttg gcctcgcggt tgaggtggat
    14341 ggccaggttg gggatcaccg cgatcggtgc cttgaagtcg atcaggcgac tgtgcagctt
    14401 gccgtcgtgc ttgaaggtga cgcgcccggc cagcgacagg tcgcggtcga accagggcgc
    14461 cagcagcatg cccccgtaga cttccacgcc cagttgccag tagccctgac gcgccagttc
    14521 cggctgcggc ttgacccgca ggcaggggct gtcggtatgc gtgccaacca ggcgcagtcc
    14581 gccctccagc ggggcgcggc ggccgagctg gagggcgatc agcgaggaat cgttgcgggt
    14641 gacgtagtag cgtccgccgg gctgtgcctg ccacggctcg cgctcgtcga gacgctggta
    14701 gccggcggcc tcgaggcggg cggccaggct ggcggtggcg tggaaggggg tcggcgaggc
    14761 cttgaggaaa tcgatcaggc cctggttgag tgcttcgcgc atgacggctc cggcggcagt
    14821 gatgcgcgga gtctaccgga atatcgccgc tgggaccgtg ctcagaacgg cgccgggctc
    14881 tcgaagcgca tccgctcgcc ggtctgcgga tgggtcaggc acagcaggct ggcgtgcagg
    14941 cacagccgct catgggcggc cagcgcctgt tcgtgggcgt acaggcggtc gccgagcagc
    15001 gggtgaccga tggccagcat gtgcacgcgc agctggtggg aacggccggt gatcggggtc
    15061 agttcgaccc ggcaccagtc gccgcagcgt tcgacgatgc gccagaaggt cagggcatgc
    15121 ttgcccagct catggtcgac gatatgccgc ggcttgttcg gcgggtcgta gcgcagcggc
    15181 agctcgatgc gcccgctgtc ggtggccggc tggccccagc acagggcggt gtaggccttt
    15241 tccgtctcgc ggtcgtggaa ctggcgcgac agttcgcggt ggctgtcggc atcgcgggcc
    15301 agcacgatca gcccggaggt ttcccagtcc aggcggtgga cgatgcgtgc ctccggatag
    15361 ccgttgtcct gcaggcgggt caccaggcag tcgcggttgt cctcggcgcg gccgggcacc
    15421 gagagcagca gggtcggctt gttgatcacc agcagggcgg cgtcctggtg gacgatttgg
    15481 atattcgaca gcggcatggg gaattccaca aatgacaacg gcggcctgct agggccgccg
    15541 tatggcacac cttgtcccga tcagcggtcg ggcagggtga tgttgagctc caggatcgag
    15601 caggtgccct ggttttccag ggcgacctgc acctgatcct ggtcgatggg cacgtacttg
    15661 cggatcaccg cgatcaactc ttcctgcagg gccggcagat agtcgggctg gccacggctc
    15721 ccgcgctcat gggcgacgat gatctgcagg cgttccttgg cgatcgaggc agaggtggtc
    15781 ttcttgcgcg agaggaggaa gtcgaagatg ctcattcacg gcctccaaac agacgttgca
    15841 gcagtccctt tttctgcact tcgaggaaac gatgcggcac ttccttgccc agcaggcgat
    15901 cgaccgcgtc gccgtaggcc tggccggcat cgctctgctc gtcgagaatc accggcacgc
    15961 cctggttgga ggccttgagc actgcctggg attcggggat cacgccgatc aggccgatgg
    16021 agaggatctc ctcgacgtcg tcgacgctga gcatttcgcc gcgggccacg cgctcggggt
    16081 tgtagcgggt cagcagcagg tgctccttga tcggctcctc gcccttttcc gcgcgctgcg
    16141 acttgctggc gagcaggccg aggatgcggt cggagtcgcg taccgaggac acttccgggt
    16201 tggtcaccac gatggcgatg tcggcgaagt acatcgccag gtgggcgccc gtctcgatgc
    16261 cggccgggga gtcgcagagg atgtattcga agtccttctt caactcctcg atgacccgct
    16321 cgacgccttc cttgttgagg gcgtccttgt cgcgggtctg gctggcggcc agtacgtaga
    16381 ggttgtcgag gcgcttgtcc ttgatcaggg cctggctcag ggtcgcctcg ttgtggatca
    16441 cgttgatgaa gtcgtaaacc acgcgacgtt cacagcccat gatcaggtcg aggttacgca
    16501 ggccgacgtc gaagtcgacg atcacggtct tgtgcccgcg cagggcgagg ccggtgccga
    16561 tggcggcgct ggaagtcgtc ttgcccacgc cacccttgcc ggaagttaca acaatgattt
    16621 tcgccaaggt gcttacccca gatgaatggt ttcaggtccc tgaattggcc gggagtatcc
    16681 gttaaaggcg ggtgatgttc aacacgtcgc ccgacaggct gacttgcacg gctttgcccc
    16741 actgcggact gcgccgcaga tcctcggcga ccttgtagcg gccggcgatg gagagcagtt
    16801 cggcagtcag ttgctggcag aagattcgcg cctcggcatt gcccttcgca ccggccaata
    16861 cgcgcccgcg cagcgggccg tatacgtgga tgttgccgtc ggcgataagt tccgcccccg
    16921 ggctgaccgc cgacagcacc accagatcgc cgccgtgggc atagatctgc tgaccgccgc
    16981 ggaccggacg ggtgatgacc agggtcggtt tcacctcggg ctcggccggt tgggctgcct
    17041 cggccggcgc tgccagtgcc ggttcggggg tcgctggctc gacgggctcg gccagtggcc
    17101 gttcgcgtcc gctggccggg ggcagcaacg gcaggccc
//
LOCUS       DATCUN010000059        10490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_152731, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000059 DATCUN010000000
VERSION     DATCUN010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10490
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152731"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="VIO83_02225"
     CDS             complement(<1..1353)
                     /locus_tag="VIO83_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY8330134.1"
                     /translation="MAVQRSLERFNLRSRLRFNIDDGQIWLDESRMLLLHAKALGALR
                     RELIDSLGAKRAHGLLFRMGFAAGQEDAERASKLLGAGENYDVFQIGPELHAFEGLVK
                     STITEAQIDWEQGTFFGEVVCENSWEAESHLQQYGIGEETACWTLAGYASGYVTRFFR
                     RFIVFRETQCTCCGDPHCVLVGKPVEAWGDDAYLEYCRPAEMDTAYQELAQELWQLRG
                     RQREQMPSGKLVGGSPAFRTAFDLLSKAAHSPITVLLLGETGVGKEVFAQWLHENSDR
                     ANQPFVAVNCAAIPNDLIESELFGVQKGAFTGAQQSRPGRFERADGGTLFLDELGDLS
                     PSAQVKLLRVLQTGEVERLGDDKTRKVNVRLVAATNVNLQQAIADGRFRADLYYRLAT
                     YPVTIPPLRDRKADIPILVSSLLEKYAPTYNKTLNGVTDRAMQALMAYNWPGNVRELE
                     N"
     gene            complement(1513..3936)
                     /locus_tag="VIO83_02230"
     CDS             complement(1513..3936)
                     /locus_tag="VIO83_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY8330135.1"
                     /translation="MSHAQSLPAEAVIADLKEFDQSSGILAERLLFNNRLAIVVICLF
                     TTLVLGYQALGLSLNAAFEKMIPTGHPYIANFLENRKQLAGMGNTLRIAVEAKEGSIF
                     DAEYLDTVRKLSDEVFLLPGVDRPYMKSLWAPAVRWTGVTEDGLDGGPVIPDDYDGSA
                     QSLEQVRINVERSGEIGQLVAANYKSSIVLVPLQERIAETGERIDYHALSQRIEQLRA
                     KYESDKVSIHVTGFAKVVGDLIDGLRQVLVFFAAAIVICTAVLYWYTRCLRSTLLVVL
                     CSMIAVVWLLGLLPTLGYELDPYSVLVPFLVFAIGMSHGAQKMNGIMQDIGRGTHRLV
                     AARYTFRRLFLAGMTALLADAVGFAVLMVIDIQVIQDLAITASIGVAVLIFTNLVLLP
                     ILLSYSGVSPVAAQRSLKAELMEANDTEHKKHPFWAFLDLFTRTPWAGLAVGLGLLLG
                     AIGLTISFQLKIGDTDPGAPELRADSRYNQDNAFMVSNYAASSDVYIVMVKTPQYACA
                     HYSTLRAVDALERELQQLPGVEATSSLAGLAKVANAGMNEGSLKWFEMPRSQEMLNAI
                     ITRAPREMFNQNCDLLTVYAYLKDHKADTLTSVVNTVEAFAAQYGSDDIRFLNAAGNA
                     GIEAATNIVVKKANVQMLFLVYAAVIVLAFITFRSWRAVVCAVVPLMITSVLCEALMV
                     WLNIGVKVATLPVIALGVGIGVDYALYVMTVTLARLKEGMSLSEAYYKALIFTGKVVV
                     LTGITLGIAVATWVWSPIKFQADMGILLAFMFVWNMLGALILLPALAHFLLRPSQTQA
                     QLQAKSVTV"
     gene            complement(3936..4949)
                     /locus_tag="VIO83_02235"
     CDS             complement(3936..4949)
                     /locus_tag="VIO83_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010567302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEY8330136.1"
                     /translation="MPDLLDLPARESARAQHRLQLAVARAGERLVAVGERGAVLLSDD
                     AGRSWRQAKSVPVSVALTDVHFASATHGWAVGHSGVVLHSADGGETWQRQLDGNQAAV
                     LVRDDARIRVEAGEEGAAAALRSAEYLVSDGPDKPFLGVRFTDELHGYVVGAYGLALH
                     TADGGKSWQSLVGRVPNPRGKHLYQVQVSGDEVLIAGEQGALFHSADAGASFAEIRTP
                     YAGTFFGALALDGDALLAFGLRGNAWRSADGGANWQRIELGQPLTISSGRQLQDGSVL
                     LADESGRLLRSTDGARSFVALAVQPGTGLTGIAEAADGALILSGARGLSRVEPDAIVS
                     GVK"
     gene            complement(5188..6543)
                     /locus_tag="VIO83_02240"
     CDS             complement(5188..6543)
                     /locus_tag="VIO83_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HEY8330137.1"
                     /translation="MHKKTALWAALAAVLMGAQGAHAAVSAEEAAALKGNLTPLGGEK
                     AANKDGSIPAWTGAQGVATSGAKVGDIPVQPFPGEKRVVQITAQNMAQYADKLSEGTQ
                     ALLKKYPDSFRVDVYPTHRTAVAPQYVYDNTAKNATQCQTKDDGKSLQGCYGGIPFPI
                     PKAGIEVVWNHLLRVENEATDVTNFKNIVLTADGSRTLATQNDQSNQYPYYYKDGSAD
                     KWSGEYFLMRFATTAPPFKAGESLVFRDSIDVAQSRQAWQYLVGQRRVRRAPTVAYDT
                     PDFVASGANYFDEVMGLMGHIDRFDWKLVGKKEMYVPYNANEVVTAKLDEAYGQHHLN
                     PDKVRWELHRVWEVEATVAGGKRHAVAKRKFYFDEDSWMVLLMDGYDSEGKLWRTTQI
                     TPFVLPSTPMLALKPAHIFNLQANTMSTAQSLNEATYTVVERKPETHYTGDAVAAEGV
                     R"
     gene            complement(6576..8228)
                     /locus_tag="VIO83_02245"
     CDS             complement(6576..8228)
                     /locus_tag="VIO83_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HEY8330138.1"
                     /translation="MDHSASLSKFRLKKGFLPIAGVCAGLSVSSAAFAFQIDSGNPDF
                     RMSWDNTVKYSAAWRVRDVDSSVADNSIGPQANTNDGDLNFDKGLISNRLDLLSEFDL
                     RYKRNYGLRLSGAAWYDDVYNKSNDNSCALGGALVNSRSADCDEFTDDTEKLHGRKAE
                     LLDAFVYGSFTPADMNLNAKAGRFTQLYGESLFFGSNGIAAAQTSLDLIKALSVPNSQ
                     FKEILRPIGQLSGQLQINSNVSVGAYYQLEWRKSRLPGAGSYFSFTDFVDEGGDSMIL
                     APGVSALRGDDIEARDSGQGGVQVKFKSGDTEYGIYAAQFHDKMPQFYVYDNGFYEQV
                     YAEDIRTVGFSFSTLIGETNVAGEMSFRDDMPLVATGNTVILPASAGADGGDNAAFPK
                     GRTMHVNLSAISVLSATPFWDGASFVGEFAFNRRLSVTDNKAQLDPEATRDAGALQFV
                     FTPEYFQVMPGVDLQVPIGLSYGMFGRSSVNGALFPANHGGNVSVGLKAEYQKTWQAS
                     VGITHYYGPDGSIIKEMGPPQLSYNNFHGDRDFVSMSIQRTF"
     gene            complement(8540..9898)
                     /locus_tag="VIO83_02250"
     CDS             complement(8540..9898)
                     /locus_tag="VIO83_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HEY8330139.1"
                     /translation="MYKKATLGVVLVGVMLGAPQGALAAVSAEEAAKLRNGTLTPLGA
                     ERAGNADGSIPPWDGGITKPVPGNMLGDIPTQLFANEKPLFQVTARNMAEYAALLSDG
                     TKALLQKYPDSFRLDVYPGHRTAAAPQVVYDNIAKNALDCSIKEGWDTIKGCIGGIPF
                     PIPQRGVEVMWNHLLRVEAPAIDYRFRNIVGGADGVHTLATRNEIAFQYPPYYQGANA
                     KDWSGEYAMFRYTTIEPPYKSGESLVARDSINGKYPRQAWQYMVGQRRVRRAPTVSYD
                     TPDFVSSGANYFDEVQGFFGALDRYEWKLVGKREMLIPYNTNGLLGAKVSEALDKHHL
                     NPDHLRWERHRVWEVEASVAEGRRHAVPTRRYYIDEDTWAIALVDGFDKKGTLWRTTQ
                     GIPYVVPAIPATLLRTSVVFNLEGNTMSVVQMLNEEDFKVVSPKSESYFTGNAVAAEG
                     VR"
     gene            10039..>10490
                     /locus_tag="VIO83_02255"
     CDS             10039..>10490
                     /locus_tag="VIO83_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330140.1"
                     /translation="MAVVRTTPKNILLIFRSQLRAIHQARIIARESSNSIIGIDLNDE
                     PRLLPEQLNTPRKSAPYSVKLDSEQLAPPPCLPLENRSCYCKFPFFVSCSDVPLPIQK
                     NEPEIYCPNGLASNTIKTSSCYRKGIKRPVSSHKVVQHGSASSPLLHRR"
BASE COUNT         1844 a         3328 c         3407 g         1911 t
ORIGIN      
        1 gttctccagc tcgcgcacgt tgcccggcca gttgtaggcc atcagcgcct gcatggcgcg
       61 gtcagtcacg ccattcaggg tcttgttgta ggtcggcgcg tacttctcca gcagcgagct
      121 gaccaggatc gggatgtccg ccttgcggtc gcgcagcggc gggatcgtca ccggataggt
      181 ggccaggcgg tagtagaggt cggcgcggaa gcgcccatcg gcaatcgcct gctgcaggtt
      241 gacgttggtc gccgccacca ggcgcacgtt gaccttgcgg gtcttgtcgt cgccgaggcg
      301 ctccacctca ccggtctgca gcacgcgcag cagcttgacc tgcgcggacg gtgacaggtc
      361 gcccagttcg tcgaggaaca gggtgccgcc gtcggcgcgc tcgaagcgac cgggtcgcga
      421 ctgctgagcg ccggtgaagg cccccttctg cacgccgaac agctcggact cgatcaggtc
      481 gttggggatc gccgcgcagt tgaccgccac gaacggctgg ttggcgcggt cgctgttctc
      541 atgcagccac tgggcgaaca cttccttgcc gaccccggtc tcgccgagca gcagcacggt
      601 gatcgggctg tgggcggcct tgctcagcag gtcgaaggcg gtgcggaacg ccggcgaacc
      661 acccaccagc ttgccgctcg gcatctgctc gcgctggcgg ccccgcagct gccagagttc
      721 ctgagccagc tcctggtagg cagtgtccat ctcggccgga cggcagtatt cgaggtaggc
      781 atcgtcgccc caggcctcga ccggcttgcc gaccagcacg cagtgggggt cgccgcagca
      841 ggtgcactgg gtttcacgaa agacgatgaa gcgacggaag aaacgggtga cgtagccgga
      901 ggcatagccg gccagcgtcc agcaggcggt ttcctcgccg atgccgtact gctgcaggtg
      961 ggattcggct tcccaggagt tctcgcagac cacctcaccg aaaaaggtgc cctgttccca
     1021 gtcgatctgc gcctcggtga tggtcgattt gaccaggccc tcgaaggcgt gcagctccgg
     1081 gccgatctgg aacacgtcgt agttttcgcc tgcgcccagc agcttggagg cacgctcggc
     1141 atcctcctgc ccggcggcaa agcccatgcg gaatagcagg ccgtgggccc gcttggcgcc
     1201 gagcgaatcg atcagctcgc ggcgcagggc accgagggcc ttggcgtgga gcagcaacat
     1261 gcggctctcg tccagccaga tctgcccgtc atcgatgttg aagcgcagtc tcgaacgcag
     1321 attgaagcgc tcgagcgagc gttgtactgc catgtcagtc atcccccgac cggctcgcga
     1381 atgggcaagg cgtgtcactg cgctcccctg cccgaccctt ggccggcttt gtcttgtttt
     1441 ggtcccgtct ggaaacggaa caggcggccg acaacttaag tcatcggccg cccggatacc
     1501 actagcgctt ggtcagaccg ttacggactt agcttgcagc tgggcctgag tctgactcgg
     1561 tcgcagcagg aagtgggcca gcgccggcag caggatcagc gcgccgagca tgttccagac
     1621 gaacatgaac gccagcagga tgcccatatc ggcctggaac ttgatcggcg accagaccca
     1681 ggtggcgacg gcgataccca gggtgatgcc ggtcagtact accaccttgc cggtgaagat
     1741 caacgccttg tagtaggcct cggacaggct catgccttcc ttcagccgcg ccagggtcac
     1801 agtcatcacg tacagcgcgt agtccacgcc gatgccgacg ccgagggcga tcaccggcag
     1861 ggtcgctacc ttcacgccga tgttcagcca gaccatcagc gcctcgcaga gtaccgaggt
     1921 gatcatcagc ggcaccaccg cgcagaccac ggcacgccac gaacggaagg tgatgaaggc
     1981 cagcacgatc accgccgcgt agaccaggaa cagcatctgc acgttggcct tcttcaccac
     2041 gatgttggtg gcggcctcga tgccggcgtt accggcggcg ttgaggaagc ggatgtcgtc
     2101 gctgccgtac tgcgcggcga aggcttccac ggtattgacc acgctggtca gggtgtcggc
     2161 cttgtggtcc ttgaggtagg cgtagacggt cagcaggtcg cagttctggt tgaacatctc
     2221 acgcggcgcg cgggtgatga tggcgttgag catctcctgc gaacgcggca tctcgaacca
     2281 cttgaggctg ccttcgttca tcccggcgtt ggccaccttg gccagtccgg ccagcgagct
     2341 ggtcgcctcg acgcccggca gctgctgcag ctcgcgctcc agcgcatcca ccgcgcgcag
     2401 ggtgctgtag tgggcgcagg cgtattgcgg ggtcttcacc atgacgatgt agacgtcgct
     2461 gctcgccgca tagttggaca ccatgaaggc gttgtcctgg ttgtagcgcg agtcggcacg
     2521 cagctcgggc gcgcccgggt cggtgtcacc gatcttcagc tggaagctga tggtcaggcc
     2581 gatggcaccc agcagcaggc cgagacccac ggccaggccg gcccacggcg tgcgggtgaa
     2641 caggtcgagg aaggcccaga acgggtgctt cttgtgctcg gtgtcgttgg cctccatcag
     2701 ctcggccttg aggctgcgct gggcggcgac cgggctgacg ccggagtagg acagcaggat
     2761 cggcagcagc accaggttgg tgaagatcag cacggccacg ccgatgctgg cggtgatggc
     2821 caggtcctgg atcacctgga tgtcgatcac catcagcacc gcgaagccca cggcgtcggc
     2881 cagcagcgcg gtcatcccgg cgaggaacag gcggcggaag gtgtagcgcg ccgcgactag
     2941 ccggtgggtg ccgcggccga tgtcctgcat gatgccgttc atcttctgcg cgccatggct
     3001 catgccgatg gcgaacacca ggaagggcac cagcaccgag taggggtcca gctcgtagcc
     3061 gagggtcggc agcaggccga gcagccagac caccgcgatc atcgaacaca gcaccaccag
     3121 cagggtgctg cgcaggcagc gggtgtacca gtagagcacc gcggtgcaga tgacgatggc
     3181 ggcggcgaag aacaccagca cctggcgcag gccgtcgatc aggtcgccga ccaccttggc
     3241 gaagccggtg acgtggatgg agaccttgtc cgactcgtac ttggcacgca gttgctcgat
     3301 gcgctgcgac agcgcgtggt agtcgatgcg ctcgccggtt tcggcgatgc gctcttgcag
     3361 cggcaccagc acgatgctcg acttgtagtt ggcggccacc agctggccga tctcgccgga
     3421 acgctcgacg ttgatgcgca cctgctcgag gctctgcgcc gagccgtcgt agtcgtccgg
     3481 aatcaccggg ccgccgtcca ggccgtcctc ggtcacgcca gtccagcgca ccgccggcgc
     3541 ccacagcgac ttcatgtacg ggcggtcgac acccggcagc aggaacactt cgtcgctgag
     3601 cttgcgcacg gtgtcgaggt actcggcgtc gaagatgctg ccttccttgg cctccacggc
     3661 gatgcgcagg gtgttgccca tgccggccag ctgcttgcgg ttctccagga agttggcgat
     3721 gtacgggtgg ccggtgggga tcatcttctc gaacgccgcg ttgagcgaca gcccgagcgc
     3781 ctggtaaccc agcaccaggg tggtgaacag gcagatgacc acgatcgcca ggcgattgtt
     3841 gaacagcagc cgctcggcga ggattccgga gctctggtcg aactccttca gatcggcgat
     3901 gactgcctcg gcgggaaggg actgggcgtg gctcatcatt tgactcccga aacaatagcg
     3961 tccggctcga cgcgggacag tccgcgcgcg ccggaaagaa tcagcgcacc atcggcggct
     4021 tcggcgatac cggtcaggcc ggtgcccggc tgtacggcca gggcgacgaa gctgcgtgca
     4081 ccatcggtgc tgcgcagcag gcgaccactc tcgtcggcga gcagcacgga gccatcctgc
     4141 agctggcgcc cggaactgat ggtcagcggc tggccgagct cgatccgctg ccagttggcg
     4201 ccaccgtcgg cgctgcgcca ggcgttgcca cgcaggccaa aggccagcag cgcatcgccg
     4261 tcgagagcga gcgcgccaaa gaaggtgccg gcgtagggcg tgcggatctc ggcgaagctg
     4321 gcgccggcgt cggcggaatg gaacagggca ccctgctcac cggcgatcag cacctcgtcg
     4381 ccactgacct gtacctggta caggtgcttg ccgcgcggat tgggaacccg cccgaccagc
     4441 gactgccagc tcttgccgcc atcggcggta tgcagggcca ggccgtaggc gccgaccacg
     4501 tagccatgca gctcatcggt aaagcgcacg ccgaggaagg gcttgtccgg accgtcgcta
     4561 accagatact cggcactgcg cagcgcagcg gcggcaccct cctcgcccgc ttcgacgcgg
     4621 atccgggcat cgtcgcgaac cagcacggcc gcctggttgc cgtcgagctg gcgctgccag
     4681 gtttcgccgc catcggcgct gtgcagcacc acgccgctat gccccaccgc ccagccgtgg
     4741 gtcgccgagg cgaaatgcac gtcggtcagc gccacgctca ccggcacgct tttggcctgg
     4801 cgccaggagc gcccggcgtc gtcggacaac agcacggcac cgcgctcacc tactgccacc
     4861 agtcgctcac cggcgcgagc gacggcgagc tgcaggcggt gttgcgcccg cgcgctttcc
     4921 cgcgcgggga ggtcgagcag gtcaggcacg gtcggcccgc ttgctgcgtt ggaagggcct
     4981 gccaggcagg cgagtgagag catggcgacg gccagggccg agcgccggcg gagcggattt
     5041 agggcgtgca tggtgcgtac tcgaatccga ctccccgcct ccgctgcgcc gtcaagcacg
     5101 gcgcctgcgg ggtgggagag tcatgagagg gaaatccggc accccgcctg gggctgaagt
     5161 gccggaactg cggccggaag gcgcgtctca gcgcacgcct tcggcggcta ccgcatcacc
     5221 ggtgtagtga gtctccggct tgcgctcgac aaccgtgtag gtggcctcgt tgagcgactg
     5281 ggcggtgctc atggtgttgg cctgcaggtt gaagatgtgc gccggcttga gggccagcat
     5341 cggcgtggaa ggcagcacga acggggtgat ctgcgtggtg cgccacagct tgccctcgct
     5401 gtcgtagccg tccatcagca ggaccatcca ggagtcctcg tcgaagtaga acttgcgctt
     5461 ggccacggcg tgacgcttgc cgccggcaac ggtcgcttcc acttcccaga cgcggtgcag
     5521 ctcccagcgc accttgtccg gattgaggtg gtgctggccg taggcctcgt ccagcttggc
     5581 ggtgaccacc tcgttggcgt tgtagggcac gtacatttcc ttcttgccga ccagcttcca
     5641 gtcgaagcgg tcgatatggc ccatcagacc catcacctcg tcgaagtagt tggcgccgga
     5701 ggcgacgaag tccggggtgt cgtaggccac ggtcggcgca cggcgcacac ggcgctggcc
     5761 gaccaggtac tgccaggcct ggcgcgactg ggcaacgtcg atgctgtcgc ggaacaccag
     5821 cgattcgcca gccttgaacg gcggggcggt ggtggcgaag cgcatcagga agtactcgcc
     5881 gctccacttg tccgcagatc cgtccttgta gtagtaggga tactggttgg actggtcgtt
     5941 ctgggtcgcc agggtgcgcg aaccatcggc ggtgaggacg atgttcttga agttggtgac
     6001 atcggtggct tcgttctcga cccgcagcag gtggttccac accacctcga tgcccgcctt
     6061 gggaatcggg aacgggatgc cgccgtagca accctgcaac gacttgccgt cgtccttggt
     6121 ctggcactgg gtggcgttct tcgccgtgtt gtcatacacg tactgcggcg ccacagcggt
     6181 gcggtgagtc gggtagacgt cgacgcggaa gctgtccggg tacttcttca gcagcgcctg
     6241 ggtgccttcg gagagcttgt cggcgtactg cgccatgttc tgcgcggtga tttgcacgac
     6301 ccgcttctcg ccggggaacg gctgcaccgg gatgtcgccg accttggcgc cggaggtggc
     6361 cacgccctgc gcgccggtcc aggccgggat gctgccgtcc ttgttggcgg ccttctcgcc
     6421 gcccagcggg gtgaggttgc ccttcagcgc cgctgcttct tccgcgctca ctgcagcgtg
     6481 cgcgccttgg gcacccatca gtacggcagc cagcgctgcc cacaaagccg ttttcttgtg
     6541 cattgttatt ctcctcgact cggggatatt ccgactcaga aagtgcgttg gatcgacatc
     6601 gaaacgaagt cgcggtcgcc gtgaaagttg ttgtaggaca gctggggcgg gcccatctct
     6661 ttgatgatcg agccgtccgg accgtagtag tgggtgatgc ccacgctggc ctgccaggtc
     6721 ttctggtatt cggccttgag gccgacgctg acgttgccgc cgtggttggc ggggaacagc
     6781 gcgccgttga ccgaggagcg accgaacatg ccgtagctca ggccgatcgg cacctgcagg
     6841 tcgacgccgg gcatgacctg gaagtactcg ggagtgaaca cgaactgcag cgccccggca
     6901 tcgcgggtgg cttcggggtc gagctgggcc ttgttgtcgg tgacgctcag gcggcggttg
     6961 aaggcgaact cgccgacgaa ggaggcgccg tcccagaacg gggtggccga cagcacgctg
     7021 atcgccgaca ggttgacgtg catggtgcgc cctttgggga aagcggcgtt gtcgccgccg
     7081 tcggccccgg cgctggccgg caggatcacc gtgttgccgg tggccaccag cggcatgtcg
     7141 tcgcggaacg acatctcgcc ggcgacgttg gtttcgccga tcagggtgct gaagctgaag
     7201 ccgacggtac ggatgtcctc ggcatacacc tgctcgtaga agccgttatc gtacacatag
     7261 aactgcggca tcttgtcgtg gaactgcgcc gcgtagatgc cgtactcggt atcgcccgac
     7321 ttgaacttga cctgcacgcc gccctgcccc gaatcgcggg cctcgatatc gtcgccccgc
     7381 agcgccgaga cgccgggcgc cagaatcatc gaatcgccgc cctcgtcgac gaagtcggtg
     7441 aagctgaagt agctgccggc acccggcaga cggctcttgc gccactcgag ctgatagtag
     7501 gcaccgaccg agacgttgga gttgatctgc agctggccgg acagctgacc gatcggacgg
     7561 agaatttcct tgaactgcga gttgggcacc gacagcgcct tgatcagatc cagcgaagtc
     7621 tgcgccgcgg cgatgccgtt ggagccgaag aacaggctct cgccatacag ctgggtgaaa
     7681 cggccggcct tggcgttcag gttcatgtcc gccggggtga agctgccgta gacgaaggca
     7741 tccagcagct cggccttgcg cccgtgcagc ttctcggtgt cgtcggtgaa ttcgtcgcag
     7801 tcggcagaac gggagttcac cagcgcgccc cccagcgcgc aggagttgtc gttggacttg
     7861 ttgtagacgt cgtcgtacca ggctgcaccg ctgaggcgca ggccgtagtt gcgcttgtag
     7921 cgcaggtcga attcggagag caggtcgagg cggttggaga tcagcccctt gtcgaagttg
     7981 aggtcgccgt cgttggtgtt ggcctgcgga ccgatggagt tgtcggcgac gctggaatcg
     8041 acatcacgca cacgccaggc ggcgctgtat ttgacggtgt tgtcccagga catgcggaaa
     8101 tccggattgc cgctgtcgat ctggaaggcg aatgccgcac tgctgacaga gaggccggca
     8161 cacacgccag cgatagggag aaatcccttt ttcaaacgaa acttcgacag actggcgctg
     8221 tggtccatgt cttgatcctc gaaaatcttt gttgttgttg tgctttcggt gccctggctt
     8281 tcttgatgcg ctgtttctgc agctcgcccc catagccttc ggacatggct gagaaactat
     8341 tacccagacg acttaccgtc caataccata aaaatgccca gccaatacct aaaaggtatc
     8401 accatgctca gccagaaacg cgcaagcctg gccgcgacag ctcaggccct tgcgcgccgc
     8461 tacctcgcca ctgctccttg tcccagagcg tccgcagggc agtcccggga tgaggtctcc
     8521 cgggcgcagt gctagcggat cagcgcacgc cctcggcagc caccgcattg ccggtgaaat
     8581 agctctcgga cttgggagac accaccttga aatcctcttc gttgagcatc tggaccacgc
     8641 tcatggtgtt tccctccaga ttgaagacca ccgaagttct cagcagcgtg gccgggatgg
     8701 caggcaccac ataggggatc ccctgggtgg ttcgccacag cgtccccttc ttgtcgaagc
     8761 catccaccag ggcgatcgcc caggtatcct cgtcgatgta gtagcgacgg gtcggcaccg
     8821 catggcgccg cccttcggcc acgctcgcct cgacctccca cacccgatga cgctcccagc
     8881 gcaggtggtc gggattgagg tggtgcttgt ccagcgcctc gctgaccttg gcgccaagca
     8941 gcccgttggt gttgtaggga atcaacattt cccgcttgcc gaccagcttc cactcgtagc
     9001 gatccagggc accgaagaac ccctgaacct cgtcgaaata gttggccccg gacgagacga
     9061 agtcgggggt gtcgtaggaa acggtcggcg cacggcgcac ccgccgctgg ccgaccatgt
     9121 attgccaggc ctggcgcgga tacttgccat tgatactgtc gcgggcgacc agcgactcgc
     9181 cggatttgta gggcggctcg atggtcgtat aacggaacat ggcgtactcg cccgaccagt
     9241 ccttggcatt ggcgccctgg taatacggcg gatactggaa ggcgatctcg ttgcgggtgg
     9301 ccagcgtatg tacgccatcg gcaccgccga cgatattcct gaagcgatag tcgatcgcag
     9361 gagcctcgac tcgcagcagg tgattccaca tcacctcgac gccgcgctga ggaataggga
     9421 aaggaattcc accgatgcag cccttgatgg tgtcccatcc ttccttgatg ctgcagtcca
     9481 gcgcattctt ggcgatattg tcgtagacaa cctgcggagc cgccgccgtt cggtggccag
     9541 gataaacatc gagccggaag ctgtcgggat acttctgcag caatgccttg gtcccgtcgg
     9601 acaacagggc agcgtattcg gccatgttcc tggcagtgac ctggaaaagc ggtttttcat
     9661 tggcaaatag ctgcgtcggg atgtccccca gcatgttgcc cggcaccggc ttggtaatgc
     9721 ccccatccca aggcggaata ctgccgtccg cattcccggc cctctcggcg cccaacgggg
     9781 tcagtgtgcc attgcgcagc ttggccgcct cctccgcgct gacagcggcc agcgcaccct
     9841 gtggcgctcc cagcatgacg ccgaccagca ccacgccaag tgttgctttc ttgtacattg
     9901 caatatcctc gactcggaga tattcgagga gaaagaggca tcggcacgac agtcaaacga
     9961 atcccggggg cgcgtaattt attcaggccg aacgcaggca tagcgccatt cacttgaagg
    10021 cactttttcc gaccataaat ggcggtagtc aggacaacac cgaaaaatat tctgctgatt
    10081 tttcgcagcc agctgcgagc catccatcag gcaaggataa tcgccagaga atcgagcaac
    10141 agcattatcg gcatcgacct gaacgacgaa ccgcgcttat tgccggaaca gctgaatact
    10201 ccacggaaat ccgccccgta ctccgttaaa ctggactccg aacaactggc gccacctcca
    10261 tgtcttcccc tcgaaaatcg ctcttgttat tgtaagtttc cgttctttgt ttcttgcagc
    10321 gatgttccgc tccccataca gaagaacgaa ccagaaatct attgcccgaa tggcttagcg
    10381 tccaatacca taaaaacgtc atcttgctac cgcaaaggta tcaagcgacc agtttcctcc
    10441 cacaaggtcg tccagcatgg atctgcgtca tctccgctac ttcatcgccg
//
LOCUS       DATCUN010000060         2710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_208957, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000060 DATCUN010000000
VERSION     DATCUN010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208957"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VIO83_02260"
     CDS             <1..374
                     /locus_tag="VIO83_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012052203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEY8330141.1"
                     /translation="ALPAVLAELAGRAAAAAGFVDFCPDACLVNRYVPGARMGLHQDR
                     DERDLRQPIVSISLGLPALFLFGGLQRSERALRVPLGHGDVLVWGGPARLRYHGVLAL
                     KEGEHPRLGAQRINLTLRRAG"
     gene            complement(417..713)
                     /locus_tag="VIO83_02265"
     CDS             complement(417..713)
                     /locus_tag="VIO83_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330142.1"
                     /translation="MLSRISLVIALLAGLALFIQAPAQASDRDGYYSHDRHAEVHRAP
                     MHYERPRYYRPAPVYYAAPRYRHERYRDDRYRHEYRHHHYRRHHRDYGYRDRRW"
     gene            complement(838..1218)
                     /locus_tag="VIO83_02270"
     CDS             complement(838..1218)
                     /locus_tag="VIO83_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009042176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY8330143.1"
                     /translation="MTDSHDQGLKVRREVMGDAFVDRAMGNATDFTAPLQEFVNAHAW
                     GGVWTREGLPRKTRSLITLAALTALKCPQELKGHVRGALNNGCTVEEIREALLHCAVY
                     AGVPAAIDAFRAAQEVIDSYQAES"
     gene            complement(1245..1646)
                     /locus_tag="VIO83_02275"
     CDS             complement(1245..1646)
                     /locus_tag="VIO83_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8330144.1"
                     /translation="MAKQTYSISDLAQELDVTTRTIRFYEEQGMLAPARRGQERIYSP
                     KDRVALKLIVRGKRLGLSLAECKTLIELYDPKTGNQKQLNIMLGMIAERRQQLEQQLL
                     DIQQMQLELDSAEERCRAALAETVGKTAAAK"
     gene            complement(1766..2395)
                     /locus_tag="VIO83_02280"
     CDS             complement(1766..2395)
                     /locus_tag="VIO83_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HEY8330145.1"
                     /translation="MAWTREQMAQRAAQELQDGFYVNLGIGLPTLVANYIPAGMDVWL
                     QSENGLLGIGPFPSEEQVDPDLINAGKQTITTLPGSSFFDSAASFAMIRGGHVNLSIL
                     GAMQVSAQGDLANWMIPGKMVKGMGGAMDLVAGVKRVVVLMEHTAKGGAHKILERCDL
                     PLTGVGVVDRIITDLAVLDVTEQGLQLVELAEGVSFEELQAATGCPIRR"
     gene            complement(2399..>2710)
                     /locus_tag="VIO83_02285"
     CDS             complement(2399..>2710)
                     /locus_tag="VIO83_02285"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004971542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY8330146.1"
                     /translation="TRQFDGEWYVMERSLTADVALVKAWKADKAGNLVFRKTARNFNP
                     LAAMAGKVCVVEVEELVETGALDADQIHLPGIYVQRIVVNASPEKRIEQRTLSNAKAG
                     S"
BASE COUNT          354 a          875 c          984 g          497 t
ORIGIN      
        1 cggcgctgcc ggcggtgctc gccgagttgg ccgggcgggc ggccgccgcg gcgggtttcg
       61 tcgacttttg cccggatgcc tgcctggtca atcgctatgt gcccggtgcg cgcatgggcc
      121 tgcatcagga ccgcgacgaa cgcgacctgc gccaaccgat cgtgtcgatt tccctcggcc
      181 tgccggcgct gttcctgttc ggcggcctgc agcgcagcga gcgggcgctg cgcgtgccgc
      241 tggggcacgg tgacgtactg gtgtggggtg gtccggcgcg cctgcgctat cacggcgtgc
      301 tggcgctcaa ggagggcgag catccgcgcc tcggcgcgca gcgcatcaat ctgaccctgc
      361 gccgggcggg ttagcgcggc gcgggtcgag atcgcctgcc gggtcacccc gcacggctac
      421 cagcggcggt cgcgatagcc gtagtcgcgg tggtggcggc ggtagtggtg atggcgatac
      481 tcatggcggt agcggtcatc gcggtagcgc tcgtggcggt agcggggcgc ggcgtagtag
      541 accggcgccg ggcggtagta gcgcgggcgc tcgtagtgca tcggcgcccg atgcacctcg
      601 gcgtggcggt cgtggctgta gtagccatcg cggtcggagg cctgggccgg cgcttgaatg
      661 aacagcgcca gaccggcgag cagggcaatg acgagggaaa tacgcgaaag cattaacggg
      721 tctcctgagg tgccggtatt gccggtcact cagtaggacc acgtcgcagg gcggaaatct
      781 cagtttgccg atgtaaaact ttgtgtcggc gccgccgccc ggctcggcgt gcccgcttca
      841 gctttcggcc tggtagctgt cgatcacttc ctgcgcggcg cggaaggcgt cgatggcggc
      901 cggcacgccg gcgtacaccg cgcagtgcag cagcgcctcg cggatctcct cgaccgtgca
      961 gccgttgttc agcgcgccgc gcacgtggcc cttgagttcc tgcgggcatt tcagcgcggt
     1021 gagggcggcg agggtgatca ggctgcgggt cttgcgcggc aggccttcgc gggtccacac
     1081 cccgccccag gcgtgggcgt tgacgaactc ctgcagcggc gcggtgaagt cggtggcgtt
     1141 gcccatggcg cggtcgacga aggcgtcgcc catcacttcg cggcgtacct tgaggccctg
     1201 gtcgtggctg tcggtcattg ctggaatcct cgagggttgg ggaattactt ggccgcagcc
     1261 gtcttgccga cggtttccgc cagcgccgcg cggcagcgtt cctcggcgct gtccagttcc
     1321 agctgcatct gctggatgtc cagcaactgc tgctccagct gctggcggcg ctcggcgatc
     1381 atgccaagca tgatgttgag ctgcttctgg ttgccggtct tggggtcgta cagttcgatc
     1441 agggtcttgc actcggccag cgacaggccg aggcgcttgc cgcgcacgat cagcttgagg
     1501 gccacgcggt ccttgggact gtagatgcgc tcctggccgc ggcgggccgg ggcaagcatg
     1561 ccctgctcct cgtagaagcg gatggtgcgg gtggtcacgt ccagctcttg ggccaggtcg
     1621 gagatgctgt aggtctgttt cgccatggga gattccggat tcgtcggcat tcgcgcccgg
     1681 cgccgagggg cgccgggcgt gtggcagtca ggagcgttcg atgcgcaagg ctacaccgtc
     1741 gccgccgcgg ctgcacaggc ggcgctcagc gccggatcgg gcagccggtg gcggcctgca
     1801 gttcctcgaa gctgacgccc tcggccagtt cgaccagttg cagaccctgc tcggtgacat
     1861 ccagcacggc caggtcggtg atgatgcggt cgaccacgcc gacgccggtc agcggcaggt
     1921 cgcagcgctc gaggatcttg tgcgcgccgc ccttggcggt gtgctccatc agcaccacca
     1981 cgcgcttgac gccggccacc aggtccatcg ccccgcccat gcccttgacc atcttgccgg
     2041 ggatcatcca gttggccagg tcgccctgcg ccgacacctg catggcgccg aggatcgaca
     2101 ggttgacgtg gccgccgcgg atcatcgcga agctcgccgc gctgtcgaag aagctgctgc
     2161 cgggcagggt ggtgatggtc tgcttgccgg cgttgatcag gtcggggtcg acctgctcct
     2221 cgctggggaa ggggccgatg ccgagcaggc cgttctcgct ctgcagccag acgtccatgc
     2281 cggcggggat gtagttggcc accagggtcg gcaggccgat gccgaggttg acgtagaagc
     2341 cgtcctgcag ctcctgggcg gcgcgctgcg ccatctgttc gcgggtccag gccatgattc
     2401 agctccctgc ctttgcgttg gaaagcgtgc gttgttcgat gcgtttttcc gggctggcgt
     2461 tgaccacgat gcgctgcacg tagatccccg gcaggtggat ctggtcggcg tccagtgcgc
     2521 cggtctcgac cagctcctcg acctcgacca cgcagacctt gccggccatc gccgccagcg
     2581 ggttgaagtt gcgcgcggtc ttgcggaaca ccaggttgcc ggccttatcg gccttccacg
     2641 ccttgaccag cgccacgtcg gcggtcagcg agcgctccat cacgtaccac tcgccgtcga
     2701 actggcgggt
//
LOCUS       DATCUN010000061         5970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_227698, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000061 DATCUN010000000
VERSION     DATCUN010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227698"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VIO83_02290"
     CDS             <1..514
                     /locus_tag="VIO83_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330147.1"
                     /translation="LGLGQLHAVDTRHELEWRLARKEAFDSLSALVQATQRSLSEPRA
                     VRPPIAPLEHLQAHSYQLLAQLSAVKSMLVLRRDRLTTADIEGPLERTAARIEAAIGS
                     TPIAGSNLPASPPATTLAGAVPLPDPFDNDVSPWLLRRLDLATAISSQLRDDAARILQ
                     SLDHPETNAP"
     gene            531..1625
                     /locus_tag="VIO83_02295"
     CDS             531..1625
                     /locus_tag="VIO83_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY8330148.1"
                     /translation="MTKDMLLGHPWFPPLLSALIAICLALIVHRLASLVLQRVTRHMP
                     VLHALLVNIKGPAQALLPLVALQAVWQAAPDDLRFIAGIRHLNGLLLIAMATWVLARA
                     VNGFADGLIAKHPYDAADNLQARRVLTQTRVLARSANSVLLVAGASMMLMTFPGARQV
                     GASLLASAGVIGIVAGLAAKPVFSNLIAGLQIALAQPIRIDDVLVVEGEWGRVEEITG
                     TFVVLRIWDDRRLILPLSYFIEKPFQNWTRTGSQLLGSVFIYADYGMPLAPLREEVER
                     LVKAAPQWDGRFFNLQVTDTTERTMQLRVLCTAANSSLAFDLRCAVREGVIGYMQRDY
                     PQFLPRLRVEDGDASPRPKAAPPDGMPQSA"
     gene            complement(1626..2546)
                     /locus_tag="VIO83_02300"
     CDS             complement(1626..2546)
                     /locus_tag="VIO83_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8330149.1"
                     /translation="MVDLNDIALFVQVVRSGSFAGAARRLGVPSNTVSRRVQALEAQL
                     GTRLLQRSTRKLTLTSAGEAFQERCASAVDGLIDAGQELMTGHHEPSGLVRVAAPADF
                     FDFFLMEWVADFLAAHPRVRLDFVLSDARADLIAERIDVAFRGGALRDSGYVGRQVLR
                     EGNDVLVASPTYLAARGSPDTLQDLKNHDCVSFAHPSGYATWRLMGSDGVDEEVQVAA
                     RFSGNTAQALRRATVAGLGIALLPHTITAFDLQADRLVPVLPHYRCVGYGLNVLYPSR
                     RHLPLAVSAFIDLVVEKLNALDASAVSQRP"
     gene            2608..3078
                     /locus_tag="VIO83_02305"
     CDS             2608..3078
                     /locus_tag="VIO83_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY8330150.1"
                     /translation="MVAILFFVHRPQKAFIYRTRHHAMTTSQPLSLTQRRIVWGLRIL
                     LALAFGAAGATKLAGVPQMVQLFDAVGFGQWFRYLTGAVEVIGAILLLVPATGFVGGL
                     LLTATMVGGVGTHLLLIGGNPAPAIVLGLLSAFVAWKLRPASLPAPSTLSSATR"
     gene            3098..3952
                     /locus_tag="VIO83_02310"
     CDS             3098..3952
                     /locus_tag="VIO83_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY8330151.1"
                     /translation="MTIATRIDTRAIVHRTRGDAHGPITRLMSPGDLGAFLKPFVFLD
                     LVRSSALTGQNFGMHPHSGIATLTFLREGDVNYADTTGKKGVLPAGGVEWMRAGNGVW
                     HTGAPVKGSPMLGFQLWVALPAAEENAPAESLYLAPADIPQEGPARVLLGRYGAAKSP
                     VPAPADMNYLAIHLKNGERWTYATPEGHTVAWLAIDTGRLDAGGMVATGELAVFEESA
                     QAIDVVAHGDTSFVLGSAVKHPHDLVMGRYSVHTSKAALAQGEQEIRRIGAELRNSGR
                     LPGTRQTN"
     gene            3997..5094
                     /locus_tag="VIO83_02315"
     CDS             3997..5094
                     /locus_tag="VIO83_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009048649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY8330152.1"
                     /translation="MTSTHLAPLFAPTQVGPLPLKNHLVMAPMTRSRAGEGDVATSTT
                     AEYYRQRAGAGLIISEGSQVSAQAKGYPRTPGIFTPAQVAGWKRVTDAVHAEGGRIFL
                     QLWHVGRLSHSSVQASGALPVAPSAIKADGELYTPTGLQPYETPRALTLAEIPGVVAD
                     FRRGAENAKAAGFDGVELHGANGYLIDQFLRDSTNARTDAYGGSVQNRIRFLQDLVEA
                     VTPVFGADRVGVRLSPLFDGFAKKDSDPQATYGHAAEMLKTYGLAYLHVMQLGTGSFD
                     FTELKRRFGGKYIANGGYDAERAAAALRSGSADLVAFGTPFLANPDLVERFRNGAALN
                     AADPSTFYQGEERGYTDYPTLHGATATQQEG"
     gene            complement(5113..>5970)
                     /locus_tag="VIO83_02320"
     CDS             complement(5113..>5970)
                     /locus_tag="VIO83_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011533915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8330153.1"
                     /translation="GSFTAAAERLGITKAVVSQQVARLEREFRTSLLVRTTRKVQATE
                     VGRAFYERCAVVLREAEHAFDGLAERSAEPSGMLRLTAPFDYGVGVVVPAIAAFTKRY
                     PACKVDAVLSDQTLDLMAGNFEMAIRVGWLAETGEQARKIGAFRQLLVASSSMRREVA
                     ALERPTDMAALPFVANTALRDHLRWRFSLDGTEDQVVQVQASIFLDATLAVREAVRQG
                     AGLSVLPDYAVAEDLAAGRLIEVLPRWHLPSGGIHAVFPAARYRPAKVRAFVDLLAER
                     EQKRQGVGR"
BASE COUNT          999 a         2147 c         1910 g          914 t
ORIGIN      
        1 gctcggcctc ggccagctgc atgcggtcga cacccgccac gaactcgaat ggcggctggc
       61 gcgcaaggag gctttcgaca gtctctcggc gctggtgcag gcgacccagc gttcgctctc
      121 cgaaccacgc gccgtgcggc cgcccatcgc accgctggaa catctgcagg cccacagcta
      181 ccagctgctg gcgcagctca gtgccgtgaa gtcgatgctg gtgttgcggc gcgaccggct
      241 cacgaccgcg gacatcgaag ggccgctgga acgcacggcc gcacgcatcg aagcggcgat
      301 cggcagcacg ccgatcgccg gcagcaatct gcccgccagc ccgcccgcca cgacgctggc
      361 cggggcggtc cccttgccgg acccgttcga caacgatgtg agtccctggc tgctgcgcag
      421 gctcgacctc gccacggcca tctcgagcca gctgcgcgac gatgcggcac gcatcctgca
      481 atccctcgac caccccgaaa ccaacgctcc ttgaaaggac cccctgaacg atgaccaaag
      541 acatgctgct cggccacccg tggttcccgc ccctcctctc tgccctgatc gccatctgcc
      601 ttgcgctgat cgtgcatcga ctggccagcc tggtgctcca gagggtgaca cgccacatgc
      661 ccgtgctgca tgctctgctg gtcaacatca aggggcccgc gcaggcgctg ctgccgctgg
      721 tcgcccttca ggcggtgtgg caggcggcac ctgacgacct gcgcttcatc gccggcattc
      781 gccacctcaa cggcctgctg ctgatcgcca tggcgacctg ggtgctggcg cgcgccgtca
      841 acggcttcgc cgacggcctc attgccaagc acccgtacga tgcggccgac aacctgcagg
      901 cgcgccgcgt gctcacccag acgcgcgtgc tggcgcgctc cgccaacagc gtgctgctgg
      961 tggcgggtgc atcgatgatg ctgatgacct tcccgggcgc gcgccaggtc ggtgccagcc
     1021 tgctggcctc ggcgggcgtg atcggcatcg tggcgggcct ggcggccaag ccggtcttca
     1081 gcaacctgat cgccggcctg cagatcgcgc tggcgcagcc gatccgcatc gacgacgtgc
     1141 tggtggtcga aggcgaatgg ggccgtgtgg aagagatcac cggcaccttc gtcgtgctgc
     1201 gcatctggga cgaccggcgg ctgatcctgc cgctgagcta cttcatcgag aagccgttcc
     1261 agaactggac gcgcacgggc tcgcaactgc tgggctcggt cttcatctac gccgactacg
     1321 gcatgccgct cgcgccgctg cgcgaagagg tcgagcgcct ggtgaaggcg gccccgcaat
     1381 gggacggccg cttcttcaac ctgcaggtga ccgacaccac cgagcgcacc atgcagctgc
     1441 gcgtgctgtg cacggcggcc aattcgagcc tggccttcga cctgcgctgc gccgtgcgcg
     1501 aaggtgtgat cggctacatg cagcgcgact acccgcagtt cctgccgcgc ctgcgcgtgg
     1561 aggacggcga tgcctcgccg aggccgaagg ccgcgccgcc ggacgggatg ccgcagtctg
     1621 cctgatcacg ggcgctgcga caccgccgat gcatccagcg cgttgagctt ctcgaccacc
     1681 agatcgatga atgccgacac ggccagcggc agatgccgtc ggctggggta cagcacgttc
     1741 aggccgtagc ccacgcatcg atagtgcggc agcaccggca ccagccggtc cgcctgaagg
     1801 tcgaaggccg tgatcgtgtg cggcagcagg gcgatcccga gcccggccac cgtcgccctg
     1861 cgcagcgcct gcgcggtgtt gccgctgaag cgggcggcga cctgcacttc ttcgtcgacg
     1921 ccatcggacc ccatcaagcg ccatgtcgca tacccgctgg gatgggcgaa gctcacgcag
     1981 tcatggttct tgaggtcctg cagcgtgtcg ggtgaaccgc gtgcagcgag gtaggtcggg
     2041 ctggccacca gcacgtcatt gccttcgcgc agcacctgac ggccgacgta gcccgagtcc
     2101 cgcagcgcgc cgccacggaa cgcgacgtct atccgctcgg caatcaggtc ggcccgcgcg
     2161 tcgctgagca cgaagtccag tcggacccgc ggatgcgcgg ccagaaagtc agcgacccat
     2221 tccatcaaga agaaatcgaa gaaatcggcg ggcgccgcga ctcgcaccag gccgctgggc
     2281 tcgtggtgac cggtcatcaa ttcctggccc gcgtcgatca atccatccac cgcgctggcg
     2341 cagcgttcct ggaatgcctc gcccgcactg gtgagggtga gcttgcgggt ggagcgctgc
     2401 agcagccgcg tgccgagttg cgcctcgagc gcctggacgc gtcggctcac cgtgttggac
     2461 ggcacgccga gccggcgcgc ggcccccgca aagctgccgc tgcgcacgac ctgcacgaac
     2521 agggcgatgt cgttcaaatc caccatggca cgcagattac tttgaaaaac ggatcagttc
     2581 aatccaattg catcacctag tcattcaatg gtggcgatcc tattcttcgt ccatcggcca
     2641 cagaaggcct tcatttaccg aacaagacat cacgccatga ccacgtcaca accgctttcc
     2701 ctcacccagc gccgcatcgt ctggggcctc cggattcttc tggccctggc cttcggcgcg
     2761 gccggcgcca ccaagctggc cggcgtgccg cagatggtgc aactgttcga cgctgtcggc
     2821 ttcggccaat ggttccgcta cctcacgggc gccgtcgaag tgatcggcgc catccttctg
     2881 ctcgtgcctg ccaccggttt cgtcggcggc ctgctcctga cggcgaccat ggtcggcggc
     2941 gtggggaccc atctgctgct gatcggcggc aaccccgcgc cagcgatcgt gctgggactg
     3001 ctctcggcct tcgtcgcctg gaaattgcgc ccggcttcgc tgcccgcccc ttccaccctt
     3061 tcaagtgcca cccgctgaca ccagcaggag caacgcaatg accatcgcaa cacgcatcga
     3121 cacgcgcgcc atcgtccacc ggacccgcgg cgacgcgcac ggcccgatca cccggctcat
     3181 gagcccgggc gacctcggcg cattcctcaa gcccttcgtg tttctggacc tggtccgctc
     3241 ctcggcgctg acgggacaga acttcggcat gcacccccat tcgggcatcg ccacgctgac
     3301 cttcctgcgc gaaggcgacg tgaactatgc ggacacgacc ggcaagaagg gcgtgctgcc
     3361 cgccggcggc gtggagtgga tgcgcgcggg caacggcgtg tggcacaccg gtgccccggt
     3421 caagggttcc cccatgctgg gctttcagct ttgggtggcc ctgcccgcgg cagaagaaaa
     3481 cgcacccgcg gagagcctct acctggcgcc ggccgacatc ccccaggaag gtccggcacg
     3541 cgtgctgctc ggacgctacg gcgcagcgaa aagcccggtg cccgcccccg cggacatgaa
     3601 ctacctggcc atccacctga aaaacggcga acgctggacg tacgcgacac cggaaggcca
     3661 caccgtggcc tggctggcga tcgacacggg tcggctcgac gcgggcggca tggtcgcgac
     3721 gggtgaactc gccgtgttcg aggaatcggc gcaggccatc gacgtcgtgg cgcacggcga
     3781 cacgtccttc gtgctgggct ccgccgtgaa gcatccgcac gacctggtca tgggccgcta
     3841 ctcggtgcac acgagcaagg ccgcccttgc acagggcgaa caggagatcc ggcgcatcgg
     3901 cgccgagctt cgcaacagcg ggcgactgcc cggcactcgc cagacgaact gaacgcagtt
     3961 cgagcgctcg atcgatccag tcaccagaaa gcatccatga cctccaccca tctcgccccc
     4021 ttgttcgccc ccacgcaggt gggcccactg ccattgaaaa accacctggt gatggcgccg
     4081 atgacgcgca gtcgcgccgg tgaaggtgac gttgcgacgt cgacgaccgc cgagtactac
     4141 cgccagcgcg ctggcgccgg cctcatcatt tcggaaggct cgcaggtctc ggcacaagcg
     4201 aagggctacc cgagaacgcc cggcatcttc accccagccc aggtcgccgg ctggaagcgt
     4261 gtgaccgacg cggtccacgc ggagggtgga cgcatcttcc tgcaactctg gcacgtcggc
     4321 cggctgtcgc attcctcggt ccaggccagc ggcgcgttgc ccgtggcgcc gtctgcgatc
     4381 aaggcggatg gcgagctcta cacccccacg ggcctccaac cttacgagac cccgagggcg
     4441 ctgacgctgg ccgagattcc aggcgtcgtc gccgactttc gccgaggcgc cgagaacgcc
     4501 aaggcggccg gcttcgatgg cgtggagctc cacggcgcga atggctacct gatcgatcag
     4561 tttctgcgcg acagcaccaa tgcccggacc gacgcctacg gcggatcggt gcaaaaccgc
     4621 atccgcttcc tccaggacct ggtcgaagcg gtgacgccgg ttttcggggc ggaccgtgtc
     4681 ggcgtccgtc tgtcgcccct cttcgatggc ttcgccaaga aagacagcga tccgcaggcg
     4741 acctacggcc acgcagccga gatgctcaag acctatgggc tggcgtacct gcatgtcatg
     4801 caactgggca cgggctcgtt cgacttcacc gaactgaagc gccgcttcgg cggcaagtac
     4861 atcgccaatg gcggctacga cgccgagcgt gctgccgcgg cacttcgttc gggttcggcc
     4921 gatctggtcg cattcggcac accgttcctg gccaatccgg acctggtcga gcggttcagg
     4981 aacggcgctg cgttgaatgc ggccgatcct tcgaccttct accagggcga agagcgcggc
     5041 tacaccgact accccaccct tcacggcgcc accgccacgc agcaagaggg ctgagcccat
     5101 cgaccggcag catcagcggc cgacgccctg ccgcttctgc tcccgctccg ccaacagatc
     5161 gacgaacgcg cgaaccttcg ccgggcgata acgcgccgcc ggaaacacgg catggatgcc
     5221 tccggaaggc aggtgccagc gcggcagcac ttcgatgagt cgcccggcgg caagatcctc
     5281 ggcaaccgcg tagtcgggga gcaccgacag ccccgcaccc tggcgcacgg cctcgcggac
     5341 ggcgagcgtc gcgtccagaa agatggaggc ctgcacctga acgacctggt cctcggtccc
     5401 gtccagggag aaacgccatc ggaggtgatc ccgcaaggcg gtgttggcca cgaacggcaa
     5461 ggcggccatg tccgtggggc gctcgagcgc cgcgacctcc cgccgcatgg acgacgacgc
     5521 gacgagcaac tggcggaagg cgccgatctt tcgcgcctgc tcgcccgtct cggccagcca
     5581 gccgacacgg atcgccatct cgaagttgcc ggccatcagg tccagcgtct ggtcgctcag
     5641 cacggcatcg accttgcacg cgggatagcg cttcgtgaac gcggcgatgg caggcacgac
     5701 cacgccgacg ccatagtcga aaggcgcggt gagccgcaac atgcccgagg gctcggccga
     5761 ccgctcggcc aggccatcga acgcatgctc cgcctcgcgc aggaccacgg cgcaccgctc
     5821 gtagaaggcc cggcccacct ccgtggcctg caccttgcgc gtggtgcgca ccagcaggga
     5881 ggtccggaac tcgcgctcca gccgagccac ctgctggctg accacggcct tggtgatgcc
     5941 gaggcgttcc gcggcggcgg tgaacgaccc
//
LOCUS       DATCUN010000062         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_152774, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000062 DATCUN010000000
VERSION     DATCUN010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152774"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VIO83_02325"
     CDS             <1..871
                     /locus_tag="VIO83_02325"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEY8330154.1"
                     /translation="YLRRLQQRIELRERRTLDAARGHDRLQAARQQAGEQIRRVLIGR
                     TLPRLIHELLESGWRDVLTLTHLRQGEQSDEWRRACLVADQLAWSCTPLDHSGRERLQ
                     RERLELLEQLRTGLQQLGSLPEDRIRRLLQDVVTCQHAVQAQQPELAARLSAQLPDSP
                     LGAMLQGQPEADLSAHVTAPGDLPKELEARLQQLQSLPFGTLFGFHEDGQQRRLRLSW
                     FSPATRHYLFIDPSGEHSRSWPAAHLAQALQDGSVRLLGEPTDTPLMERALQAIYRVL
                     QRMEDGKALPADS"
     gene            complement(1023..3587)
                     /gene="clpB"
                     /locus_tag="VIO83_02330"
     CDS             complement(1023..3587)
                     /gene="clpB"
                     /locus_tag="VIO83_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY8330155.1"
                     /translation="MRIDRLTSKLQLALSDAQSLAVGRDNSAIEPLHLMSALLEQQGG
                     SIRPLLLQVGFDIAALRQALNQALDDLPKIQNPTGDVNMSQDLARLLNQADRLAQQKG
                     DQFISSELVLLAALESGTKLGKLLLAQGVSKKALENAISNLRGGEAVNDPNAEESRQA
                     LDKYTVDMTKRAEEGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPDGLKDKRLLSLDMGALIAGAKFRGEFEERLKAVLNELGKQEGRVILF
                     IDELHTMVGAGKAEGSMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVLVDEPSEEDTIAILRGLKERYEVHHGVTITDGAIIAAARLSHRYITDRQLPDKAID
                     LIDEAGSRIRMEIDSKPEELDRLDRRLIQLKIEREALKKETDEATKKRLAKLEEDIAK
                     LEREYADLEEIWKSEKAEVQGSAQIQQQLEQARADMEAARRRADLQRMAELQYGTIPE
                     LERKLATVDEREPREHQLLRNRVTEEEIAEVVSKWTGIPVAKMMEGERDKLLRMESEL
                     HKRVIGQHEAVVAVSNAVRRSRAGLADPNRPSGSFLFLGPTGVGKTELCKALAEFLFD
                     TEEAMVRIDMSEFMEKHSVSRLIGAPPGYVGYEEGGYLTEAVRRKPYSVILMDEVEKA
                     HPDVFNVLLQVLEDGRLTDSQGRTVDFKNTVVVMTSNLGSAQIQELVGDPEAQRAAVM
                     DAVSHHFRPEFINRIDEVVVFDPLGKEQIGGIAEIQLGRLRSRLAERELSLELTPEAL
                     EKLVAVGYDPVYGARPLKRAIQRWIENPLAQQILAGEFAPGATIHAKVEGEEIVFA"
BASE COUNT          535 a         1266 c         1165 g          635 t
ORIGIN      
        1 gtacctgcgt cgcctgcagc agcgcataga gctacgcgag cggcgcaccc tggatgccgc
       61 gcgcggccac gaccgcttgc aggccgcccg acagcaggcc ggcgagcaga tccgtcgggt
      121 actgatcggc cggacgctgc cacgcctgat tcacgagctg ctggagagcg gctggcgcga
      181 cgtactgacc ctgactcatc tgcgccaggg cgagcagagc gatgaatggc gacgcgcctg
      241 cctggtcgcc gatcaactgg cctggagctg tacaccactg gaccacagcg gccgcgagcg
      301 cctgcagcgc gaacgcctgg agctgctgga gcaactgcgc acaggcctgc agcaactggg
      361 cagcctgccc gaagaccgca tccgccgcct gctgcaggat gtggtcacct gccagcatgc
      421 ggtgcaggca cagcagccgg agctggccgc ccgcctgagc gcgcagctgc cggacagccc
      481 gcttggcgcc atgctgcagg gacagccgga agccgacctg tccgcccacg tcaccgcgcc
      541 cggagacctg ccgaaggaac tggaggctcg cctgcaacag ctgcagtcac tgccgttcgg
      601 cactctgttt ggcttccatg aggacggcca gcagcgccgc ctgcgcctgt cctggttcag
      661 cccggcgacc cgacactacc tgttcatcga ccccagcggc gaacacagcc gcagctggcc
      721 cgccgcccac ctggcccagg cgttgcagga cggcagcgtg cgcctgctcg gcgaaccgac
      781 agatacgccg ctgatggagc gcgccctgca ggcgatctat cgggtcctgc aacggatgga
      841 agacggcaaa gcattaccgg ccgatagctg accccgtcga cctgccgccc ccacaaacaa
      901 caagggcacg acgccccaaa gcctcgtgcc cttattgctt gcatccacac cttgacgcgc
      961 cggagcgacc cacctccatc gccacagctt ggcgcccgag gggcgccaag ctgtgttcgc
     1021 ggtcaggcga acacgatctc ctcgccctcg accttggcat ggatagtcgc ccccggcgcg
     1081 aactcgccag cgaggatctg ctgcgccagt gggttctcga tccagcgctg gatcgcgcgc
     1141 ttgagcgggc gcgcgccgta caccgggtcg tagccgacgg cgaccagttt ctccagcgcc
     1201 tccggggtca gctccaggct cagctcgcgc tcggccaggc gcgaacgcag gcgaccgagc
     1261 tggatctcgg cgatgccgcc gatctgctcc ttgccgagcg ggtcgaacac caccacctcg
     1321 tcgatgcggt tgatgaattc cggacggaag tggtggctca ccgcatccat caccgccgcg
     1381 cgctgcgctt ccggatcgcc gaccagctcc tggatctgcg ccgagccgag gttggaggtc
     1441 atcaccacca cggtgttctt gaagtccacg gtgcggccct gactgtcggt caggcggccg
     1501 tcctcgagca cctgcagcag cacgttgaac acatccggat gggccttctc cacctcgtcc
     1561 atgaggatca ccgagtaggg cttgcggcgc accgcctcgg tcaggtaacc gccctcctcg
     1621 tagccgacgt agcccggcgg ggcgccgatc aggcgggaca ccgagtgctt ctccatgaac
     1681 tcggacatgt cgatgcgcac catggcctcc tcagtatcga agaggaactc ggccagcgcc
     1741 ttgcatagct cggtcttgcc cacgccggtc gggccgagga acaggaagga gccgctcggc
     1801 cggttgggat cggccagccc ggcgcgcgaa cggcgtaccg cgttggacac cgccaccacc
     1861 gcctcgtgct ggccgatcac ccgcttgtgc agttcgctct ccatgcgcag cagcttgtcg
     1921 cgctcgccct ccatcatctt ggccaccggg atgccggtcc acttggacac cacctcggcg
     1981 atttcttcct cggtgacgcg gttgcgcagc agctggtgct cgcgcggttc gcgctcgtcg
     2041 accgtggcca gcttgcgctc cagctcgggg atggtgccgt actgcagctc ggccatgcgc
     2101 tgcaggtcgg cgcggcggcg cgccgcctcc atgtcggccc tggcctgctc cagctgctgc
     2161 tggatctgcg ccgagccctg cacttcggcc ttctccgatt tccagatctc ctcgaggtcg
     2221 gcgtactcgc gctccagctt ggcgatgtcc tcttccagct tggccaggcg cttcttggtc
     2281 gcctcgtcgg tttccttctt cagcgcctcg cgctcgatct tcagctggat caggcggcga
     2341 tccaggcggt ccagctcctc aggcttggag tcgatttcca tgcggatgcg gctgccggcc
     2401 tcgtcgatca ggtcgatggc cttgtccggc agctggcggt cggtgatgta gcgatgggag
     2461 agcctggcag cggcgatgat cgcgccgtcg gtgatggtca ccccgtggtg cacctcgtag
     2521 cgctctttca agccacggag gatggcgatg gtgtcctcct cgctcggctc gtcgaccagc
     2581 accttctgga agcggcgctc cagcgcggcg tccttctcga tgtactggcg gtactcgtcg
     2641 agggtggtgg caccgacaca gtgcagctcg ccgcgcgcca gcgccggttt gagcatattg
     2701 ccggcatcca tcgagccctc ggccttgccg gcgccgacca tggtgtgcag ctcgtcgatg
     2761 aacaggatga cccggccttc ctgcttgccc agttcgttga gcaccgcctt gaggcgctcc
     2821 tcgaactcgc cgcggaactt ggcgccggcg atcagcgcgc ccatgtccag cgacagcagg
     2881 cgcttgtcct tgaggccgtc gggcacctcg ccgttgacga tgcgctgggc caggccctcg
     2941 acgatcgcgg tcttgcccac gcccggctcg ccgatcagca ccgggttgtt cttggtgcgc
     3001 cgctgcagga cctggatggt acggcggatc tcgtcgtcgc gaccgatcac cgggtcaagc
     3061 ttgccctcct cggcgcgctt ggtcatgtcg acggtgtact tgtcgagcgc ctgacgcgac
     3121 tcctcggcat tgggatcgtt gaccgcctcg ccgccgcgca ggttgctgat ggcattttcc
     3181 agcgccttct tgctcacgcc ctgggccagc agcagcttgc ccagcttggt gccgctttcc
     3241 agcgcagcga gcagcaccag ctcgctggag atgaactggt cgcccttctg ctgggccagg
     3301 cgatcggcct gattgagcag gcgcgccaga tcctgcgaca tgttcacgtc gccggtgggg
     3361 ttctggatct tcggcaggtc gtcgagagcc tggttcagcg cctggcgcag ggcggcgatg
     3421 tcgaagccga cctgcagcag cagcgggcgg atcgagccac cctgctgctc gagcagagcg
     3481 gacatcaggt gcagcggctc gatggcgctg ttatcgcggc ccacggccag ggactgggcg
     3541 tcggaaagcg ccagttgcag tttgctggtc aaacggtcga tacgcatggg ttcgtccttc
     3601 g
//
LOCUS       DATCUN010000063        28563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_190250, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000063 DATCUN010000000
VERSION     DATCUN010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 28563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28563
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190250"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..3593)
                     /locus_tag="VIO83_02335"
     CDS             complement(54..3593)
                     /locus_tag="VIO83_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8330156.1"
                     /translation="MNRLFTLRGLLLSAFLGFGLLVSVYQLVSVHRHSLQEAMSELET
                     TTDFIGHFLAGGLELNGSASIDQLGHLFAEHLNHPALVYAAVLDSGGRMRSLSLPQGV
                     TRMPLSVPAIERLLGERAGLEGSHLHWLEEGDLLLGGYPLGTSTGLAGQGVPDMVVLA
                     MRTDTLRAHAWSRTGKQLLLLLLPLLLLALLLAWLLKRLLTDRIEELLQDVRRMSSGR
                     LGTLPLLGNDEVGQLAAEFAALMQRVVTARDYNEYLLNCIPSPVWRVDAERTCVYVNA
                     AWLQFTGMSRTDSLLEGWWQAVHEEDRHLLEPLLRAGGDGFGKGFSSRLRLRSRTGHF
                     HWMQLHGEPLVGHDGRFHGYIGSCFDMQAELDAGAELMAKEARFRGLVERSLVGVYVL
                     RDGHFEYVNPRMAEWFGYQADDLVARALLDLVGEGSRAEVGSMLARCESGERASVHFE
                     FRALTRDGREFMAEAFGTRIELDGLPAIIGTLLDVSQRYRDRETIQAVVDAVVASPMV
                     LWRRKLENGWPVQYVSDNVERWGYSAEQLLDGSRWFTTLIHPDDLALVLGEVERCIHE
                     RCPEFVLEYRIRTGVGSYIWIEDYSRLHRDTAGEVQYLEGLLIDISARKATDEKLRHA
                     AAVFTNAREGVVITDTSPRIIAVNRAFCDITGYEEHEVLGCNPSIVSSGRQSKEFYRD
                     MWAALLDEGHWQGEVWNRRRSGEVFPQWHSISAVRDDKGKLTNYVAVFSDISQLKESE
                     AQLERLAHYDPLTGLPNRLLVQSHLNGAIERARRLHCKVAVLFLDLDGFKRVNDSLGH
                     SVGDELLVLIARRLRQRIRAEDMFARLGGDEFLLVLESIHHPEEAATVAQDLINLLQQ
                     PFRAGSGHELFVGASIGISLFPDDGQEMAELIRCADVAMYQAKENGRNSYHFHTKELT
                     RAAENRQALESRLHRAITAQEFVLYYQPQVDVASGRILGFEALVRWQPESGEPVSPGV
                     FIPLLEETGLILALGDWVLHEACRQALRWHEAGLFDVPVSVNLSPHQFQHLDVVELVR
                     RALEHSGLPGALLELEITEGALMAQDLTTQARLSELKALGVRLAVDDFGTGYSSLAYL
                     SRFPLDKLKIDKSFIDGLGQDERADKIVATIIAMGQGLDLQVIAEGVESAGQLQLLRG
                     LGCDAVQGYLCGRPLPAEQAAARLAQERQALPA"
     gene            complement(3819..3897)
                     /locus_tag="VIO83_02340"
     tRNA            complement(3819..3897)
                     /locus_tag="VIO83_02340"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3859..3861),aa:Arg,seq:cct)
     gene            4194..4511
                     /locus_tag="VIO83_02345"
     CDS             4194..4511
                     /locus_tag="VIO83_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HEY8330157.1"
                     /translation="MKQSPASRSSRTADKFVVRLPEGMRSRIAEVARHYHRSMNSEII
                     ARLEKTLLAEGNLPDAAANAPVHGELHPHEHQLLRHFRQLPQPQQSALIALLGSSPGT
                     QKS"
     gene            complement(4577..6019)
                     /gene="mgtE"
                     /locus_tag="VIO83_02350"
     CDS             complement(4577..6019)
                     /gene="mgtE"
                     /locus_tag="VIO83_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY8330158.1"
                     /translation="MSEAEAKKPQESLQDRLAQVIDLLHRQRLVEQLAHRQEGGHHEL
                     VENLVHRQNLAELQRKLDDLHPADIAHILEALPLDDRLTVWQLVKAERDGDILLEVSD
                     AVRETLIADMDDHEILAATRDLDADELADLAPELPRDVVHELMETLDAQQRERVRSAL
                     SYEEDQVGALMDFEMVTIRDDVTLEVVLRYLRRLKELPGHTDKLFVVDYDGMLQGVLP
                     IKRLLVNDPDKKVAEVMATDPVGFQPDEDAYEAAQAFERYDLISAPVVDKSGKLIGRL
                     TIDEMVDLIREESESEVLNMAGLREEEDIFASVWKSVRNRWAWLALNLITAFVASRVI
                     GLFEGSIEKLVALAALMPIVAGIGGNSGNQTITMIVRAMALDQVGTGNTMRLLRKELG
                     VGLLNGVVWGGVIGSVAWYLYGSWSLGAVMTGAMTLNLLLAALMGVLIPMTLARFGRD
                     PAMGASVMITAVTDSGGFFIFLGLATLFLL"
     gene            complement(6340..6416)
                     /locus_tag="VIO83_02355"
     tRNA            complement(6340..6416)
                     /locus_tag="VIO83_02355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6380..6382),aa:Arg,seq:acg)
     gene            complement(6481..6557)
                     /locus_tag="VIO83_02360"
     tRNA            complement(6481..6557)
                     /locus_tag="VIO83_02360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6521..6523),aa:Arg,seq:acg)
     gene            complement(6670..6760)
                     /locus_tag="VIO83_02365"
     tRNA            complement(6670..6760)
                     /locus_tag="VIO83_02365"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6724..6726),aa:Ser,seq:gct)
     gene            complement(6833..7018)
                     /gene="csrA"
                     /locus_tag="VIO83_02370"
     CDS             complement(6833..7018)
                     /gene="csrA"
                     /locus_tag="VIO83_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEY8330159.1"
                     /translation="MLILTRRVGETLMVGDDVTVTVLGVKGNQVRIGVNAPKEVAVHR
                     EEIYQRIQKEKDEEPSH"
     gene            complement(7205..8440)
                     /locus_tag="VIO83_02375"
     CDS             complement(7205..8440)
                     /locus_tag="VIO83_02375"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY8330160.1"
                     /translation="MALIVQKFGGTSVGSVERIAQVAEKVKKFREAGDDIVVVVSAMS
                     GETNRLIDLAKQIMPQPDPRELDAIISTGEQVTIGLLAMALIQRGVPAVSFTGNQVRI
                     LTDSAHTKARILEIDDQRLRAELKAGRVVVVAGFQGVDETGAITTLGRGGSDTTGVAL
                     AAALKADECQIYTDVDGVYTTDPRVVQRARRLEKITFEEMLEMASLGSKVLQIRSVEF
                     AGKYNVPLRVLHSFQEGPGTLITIDEEESMEQPIISGIAFNRDEAKLTIRGVPDTPGV
                     AFKILGPISAANVEVDMIVQNVAHDNTTDFTFTVHRNDYDKALGVLKQTAAAMGAREV
                     SGDTEIAKVSIVGVGMRSHAGVASRMFEALAKENINIQMISTSEIKVSVVVEEKYLEL
                     AVRALHTAFDLDAPARQGE"
     gene            complement(8540..11164)
                     /gene="alaS"
                     /locus_tag="VIO83_02380"
     CDS             complement(8540..11164)
                     /gene="alaS"
                     /locus_tag="VIO83_02380"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY8330161.1"
                     /translation="MKSAEIREAFLRFFEEKGHTRVASSSLIPANDPTLLFTNAGMNQ
                     FKDCFLGLEKRAYTRATTSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RDAISYAWEFLTSPKWLNLPADKLWVTVYASDDEAYDIWTKDVGVPAARMVRIGDNKG
                     APYASDNFWAMGDTGPCGPCTEIFFDHGEHIWGGPPGSPEEDGDRYIEIWNNVFMQFN
                     RTADGVLHPLPAPSVDTGMGLERISAVLQHVNSNYEIDLFQSLLNASAAAIGCANEGQ
                     ASLKVVADHIRSCGFLVADGVTPSNEGRGYVLRRIVRRACRHGNKLGAKGSFFHKIVA
                     ALAAEMGEAYPELRQQQAHIERVLKAEEEQFAKTLEQGLKILEQDLAELQGKIIPGDV
                     VFKLYDTYGFPVDLTADIARERELSIDEEGFEREMQAQRERARSASAFDLDYNSLIKV
                     EGETRFTGYAGTRGAGRVLALFKEGMNVDSLNAGEEGVVVLDQTPFYAESGGQAGDCG
                     YLEANGLRFDVRDTRKAGGAFLHHGILDAGSLSVGDQVEAVVDATVRGATALNHSATH
                     LLHAALRQILGEHVGQKGSLVDSQRLRFDFSHFEAIKPEQLKALEELVNAEIRKNSEV
                     ETEETDIDTAKAKGAMALFGEKYGDSVRVLSMGGGFSVELCGGTHVSRTGDIGLFKIV
                     SEGGVAAGVRRIEAVTGAQALDYLNAAEEQLKDAAALVKGSRDNLLDKLGGLLERNRQ
                     LEKELEQLKAKAASAAGDDLAGAAIEVAGVKVLATRLDGLDGKALLALVDQLKNKLGR
                     AVVLLGGEFDGKVVLVAGVTQDLTAKLKAGELMKQAAAVVGGKGGGRPDMAQGGGVDA
                     GKLDEALALVEPFVAAAF"
     gene            complement(11326..12333)
                     /gene="astE"
                     /locus_tag="VIO83_02385"
     CDS             complement(11326..12333)
                     /gene="astE"
                     /locus_tag="VIO83_02385"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017902910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HEY8330162.1"
                     /translation="MLALGKLLELTLAGREPSEKIQVCASGARLQWKAEGVLLIDPPA
                     GRDTGLDLLLSAGIHGDETAPVELLERLLHDIASCRLQPVVRLLVVLGNPAALRRGQR
                     YVEYDLNALFGGGEPSVSGADALRAVELEQQVELFFSPPGRRRMHYDLHAAIRASRIE
                     RFALRPRSAQPLSRGQLAGLQAAGLQALLLQSEPAATFSAFTSERFAAEAFTFELGQA
                     RPLGQGGEVELSRLEAWLRGLLEGREPAMDGDPAALPLFRIAREVIKHSDAFHLHLGE
                     DVASFTELLPGSLLAEDAEGRRWLVEEQGARIVFPSPRVASGQCAGLIVVPVAVDEVC
                     S"
     gene            complement(12398..12688)
                     /locus_tag="VIO83_02390"
     CDS             complement(12398..12688)
                     /locus_tag="VIO83_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY8330163.1"
                     /translation="MSESLPLILEDAHGERRETHSARIGVRLEGRELWLEADGMGGVT
                     IYAESVEGETEIPLLVVRPQAVNQLSLSVELEAAEAHEHDHEHDADCGCDHH"
     gene            complement(12804..13832)
                     /gene="astA"
                     /locus_tag="VIO83_02395"
     CDS             complement(12804..13832)
                     /gene="astA"
                     /locus_tag="VIO83_02395"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEY8330164.1"
                     /translation="MIVRPVRSTDLPALIAMARSTGAGLTSLPASEDRLAQRVAWAEK
                     SFHGEAERADADYLFVLETDAGEVVGISAMNGAVGLREPWYNFRVGLTVSASRALGIH
                     RQNPTLFLANDLTGNTEVCSLFLDANYRHSGLSGRLLSKARFLFIAEFPELFGDKVIA
                     EMRGISDDNARAPFWESLGRHFFKMEFSQADYLTGVGNKAFIAELMPKFPLYTCFLSE
                     EARAVIGRVHRDTEPALTMLRAEGFTYQGYIDIFDGGPALECETRSIRAVRDSQALVL
                     AIGTPGEEAPQFLIHNRKREDCRITVGKARPASGTLVVDAATAKRLRLSAGAQVRAVP
                     LSAQVGWR"
     gene            complement(13947..14963)
                     /gene="aruF"
                     /locus_tag="VIO83_02400"
     CDS             complement(13947..14963)
                     /gene="aruF"
                     /locus_tag="VIO83_02400"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007971023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/ornithine succinyltransferase subunit
                     alpha"
                     /protein_id="HEY8330165.1"
                     /translation="MLIMRPARMGDLPEVQRLAADSPVGVTSLPDDRERLAEKIRASE
                     ASFAAEVSFNGEESYFFVLEDTASGKLVGCSGIVATAGFSEPFYSFRNETFVHASREL
                     QIHNKIHVLSMCHDLTGNSLLISFYLQPELESTPWAELNSRGRLLFVASHPERFAESM
                     VVEVVGYSDDKGDAPFWDAIGRNFLAIGYAEAERLCGLKGRTMLAELMPSYPIYVPLL
                     PDSAQEAMGQVHPRSQVSFDILMREGFESEHYVDIFDGGPTVHARTAGLRSIAQSRLV
                     PVRLGAPAQSGRAYLVANNQLQDFRALIVELDWEVGQPLTLTPALAEALGVGEGGSVR
                     VVAL"
     gene            complement(14995..16215)
                     /locus_tag="VIO83_02405"
     CDS             complement(14995..16215)
                     /locus_tag="VIO83_02405"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019093134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY8330166.1"
                     /translation="MSVPHAPVQRADFDQLMVPVFAPAAFVPVRGEGSRVWDQSGREL
                     VDFTGGIAVNVLGHAHPALVEALTEQAQKLWHVSNVFTNEPALRLAKLLTEATFADRV
                     FFANSGAEANEAAFKLARRYGHEVAGPQKCEIISALNSFHGRTLFTVSVAGQPKYSSG
                     FGPQIEGISHVPYNDLEALKAQISANTCAVVLEPIQGEGGVLPADQAYLEGVRALCDE
                     HNALLIFDEVQTGVGRTGQLFGYMHYGVTPDILTSAKSLGGGFPISAMLTTDAVAKHF
                     GVGVHGTTYGGNPLACAVGEAVISIVNTPEVLSGVGARSAHFTRELLRLGEQYGVFSQ
                     VRGMGLLLGAVLTEAWKGRAGEVMAAAQQQDLMILQAGPDVVRFAPSLVISEADIDEG
                     MARLERALASLTAA"
     gene            complement(16459..17436)
                     /locus_tag="VIO83_02410"
     CDS             complement(16459..17436)
                     /locus_tag="VIO83_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015885478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEY8330167.1"
                     /translation="MNPVHLAFLYWPDVRPLTLTLIDEALQLAMRLHPEALYTVSFLQ
                     AAPVEGDGWALPGQPWQERLEGSERLFLLAEEPPELAPELATALRQLARHGGLLGAVG
                     AGLYPLAQLGLLDGHRVALHWRWQDDFSERFPLVGATNHLFEWDRERLTACGGLAVLD
                     MLLAMLTRDHGAELAGAIAEELVLERIREGGERQRVPLRNRLGAAHPKVTQAVLLMEA
                     NIEEPLTTDAIAQHVCVSRRQLERIFKQFLNRVPSQFYLELRLNRARQLLQQTSTSII
                     QIGLSCGFSSGPHFSSAYRNFFGVTPREDRNLSRAAATGEVGGGLGEHG"
     gene            complement(17547..17960)
                     /gene="arfB"
                     /locus_tag="VIO83_02415"
     CDS             complement(17547..17960)
                     /gene="arfB"
                     /locus_tag="VIO83_02415"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY8330168.1"
                     /translation="MLEISNSVHLPDSEVELTAIRAQGAGGQNVNKVSSAVHLRFDIH
                     ASSLPAFYKERLLALRDSRITGDGVIVLKAQQYRTQEQNRADALERLVELIRSVAKVE
                     KARRPTRPTFGSKQRRLEGKSKRAVIKSGRGKVDF"
     gene            complement(18141..19643)
                     /gene="mqo"
                     /locus_tag="VIO83_02420"
     CDS             complement(18141..19643)
                     /gene="mqo"
                     /locus_tag="VIO83_02420"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HEY8330169.1"
                     /translation="MMQTGSDQPDLVFVGAGVMSATLAVLLKELDPSLKIEVLEQLES
                     GAAESSFPWNNAGTGHAALCELNYTPEGPDGSVGIAKAVSINTMFEVSRQLWAYLVEK
                     GYFKSPKDFISPVPHMSFVRGEKGVSFLRKRFDAMKAHHCFSDDLEYTEDRQTIGQWA
                     PLLMQGRTTNEPLAATRAMGGTDVNFGALTKKLLQHLEQSEGCKVAYNQKITDLRREA
                     DGRWRLSVDDVARGGSREVTARFVFLGAGGAALTLLQKSGIEEGKGFGGFPVSGQWLR
                     CDNPEIVKQHQAKVYSQAEIGAPPMSVPHLDTRVVDGGKSLLFGPFAGFSTKFLKQGS
                     FLDLPLSIRPNNIGPMLAVARDNMDLTKYLIKEVLQSEDQRLEELRKFFPAAKKEDWR
                     LEIAGQRVQIIKKDAKKGGVLQFGTEIVNAADGSLAALLGASPGASVSVSVMLEVIER
                     CFPEQYRSAAWQERLGQIFEADEETLQKDAELLRRVRARTAKVLELNQPG"
     gene            complement(19985..20290)
                     /locus_tag="VIO83_02425"
     CDS             complement(19985..20290)
                     /locus_tag="VIO83_02425"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY8330170.1"
                     /translation="MFLAMNRFKIVRGHEETFIDHWRQRESYLDEVPGFLRFHLLKGP
                     QTEEYTLFSSYSEWESAQSFEDWTHSESFRKAHANAGKSPREIYLGPPQLELFDSVL"
     gene            complement(20358..22154)
                     /locus_tag="VIO83_02430"
     CDS             complement(20358..22154)
                     /locus_tag="VIO83_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8330171.1"
                     /translation="MPDGGDSRDRLSWAEIRRLALGHRRELLIANLVAVLATLCSVPI
                     PLLLPLLVDEVLLGQGDAALQVMNRLLPVAWQQAAGYIGLMLLATLLLRGASLAFNVL
                     QARLFAGLSKDVVYRLRLRLIERLRRISLAEYESLGSGTVTAHLVTDLDTLDKFVGDT
                     LSRLLVALLTLAGTSAILIWMHWQLALLILLFNPLVIYATVQLGKRVKHLKKLENDST
                     ARFTQALGETLEAIQELRAGNRQGFFLGRLGTRAREVRDYAVASLWKSDAATRASGLL
                     FQFGIDVFRAAAMLTVLFSDLSIGQMLAVFSYLWFMIGPVEQLLGLQYAYYAAGGALS
                     RLNELLARADEPQYPGGADPFAGQTTVGIEVQGLRFAYRDEPVLDGLDLRIAPGEKVA
                     IVGASGGGKSTLVQLLLGLYAPQAGTIRFGGATLEEIGLERVREQVAVVLQHPALFND
                     TVRANLSMGREGDDGACWRALEIAQLADTVRALPQGLDSVVGRSGVRLSGGQRQRLAI
                     ARMVMAEPKVVILDEATSALDAATEYALHQALGRFLQGRTTLIIAHRLSAVKQADRVL
                     VFDGGRVAEEGGHQELIAEGGLYARLYGNLQH"
     gene            complement(22147..22782)
                     /locus_tag="VIO83_02435"
     CDS             complement(22147..22782)
                     /locus_tag="VIO83_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018929864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY8330172.1"
                     /translation="MNARLLYVMDPMCSWCWGFAPVFDALAALAQSADVSLHLVAGGL
                     RPGTAQALDGASRDYILKHWRAVAERTGQAFRFDGALPEGFVYDTEPASRALVALRSL
                     AADRVWPLVQAIQQAFYVEGRDVTRPAVLVELAEQLGVPRQAFAAAFDSAAQRAATQA
                     DFAWAQNLGIAGFPTLLAERNGQLALLTNGYQPLETLAPLLGRWLERRAYA"
     gene            complement(22872..23804)
                     /locus_tag="VIO83_02440"
     CDS             complement(22872..23804)
                     /locus_tag="VIO83_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009407537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEY8330173.1"
                     /translation="MSQNIVVAALYKFVTLEDYVALREPLLAAMLENGVKGTLLLAAE
                     GINGTVSGSRAAIDALLAWLHADPRLVDIDHKESYCSEQPFYRTKVKLKQEIVTLGVP
                     GVDPSQAVGTYVEPQDWNALISDPEVLLIDTRNDYEVAIGTFAGAIDPQTKSFREFPE
                     YVQQHFDPAKHKKVAMFCTGGIRCEKASSYMLGQGFAEVFHLKGGILKYLEEVPQAQS
                     LWRGDCFVFDNRVTVRHDLSEGDYDQCHACRNPVSVADRQNEHYVAGISCPHCWDSLS
                     EKTRASARERQKQIDLARARHQPHPIGRNPRQAD"
     gene            complement(23936..24250)
                     /locus_tag="VIO83_02445"
     CDS             complement(23936..24250)
                     /locus_tag="VIO83_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEY8330174.1"
                     /translation="MSMQEQIQQALAALEPQHLEVLNESHMHSRGQESHFKAVVVSPL
                     FAGLNAVKRHQKVYASLGALMGSFHALALHTYTPEEWAAQGGAPDSPTCMGGSKHDHH
                     SH"
     gene            24413..25807
                     /locus_tag="VIO83_02450"
     CDS             24413..25807
                     /locus_tag="VIO83_02450"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY8330175.1"
                     /translation="MSRTETDSLGPIEVATEAYWGAQTQRSLINFAIGSERMPLAVIH
                     ALALIKKAAARVNARSGDLPADIAALIEQAADEVLAGRHDDQFPLVVWQTGSGTQSNM
                     NVNEVIAGRANELAGQGRGGKQPVHPNDHVNRSQSSNDCFPTAMHIAVVLALHEQLLP
                     ALHELQGGLQAQATRHAALVKTGRTHLMDATPITFGQELSAFVAQLDYADRALHAALP
                     AVCELAQGGTAVGTGLNAPEGFAEAMARELSELAEQLFVSAPNKFAALAGHEPLVALS
                     GALKTLAVALMKLANDLRLLGSGPRAGFAEVRLPANEPGSSIMPGKVNPTQCEALSML
                     ACQVLGNDATVSFAASQGHLQLNVFKPVIVHNLLQSIRLLGDGCRNFNQHCIAGLEPD
                     AARMAEHLERGLMLVTALNPHIGYDKAAQIAKKAYAEGTSLRQAALALGYLSNEDFDN
                     WVRPEQMLAPGAQG"
     gene            25970..26176
                     /locus_tag="VIO83_02455"
     CDS             25970..26176
                     /locus_tag="VIO83_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HEY8330176.1"
                     /translation="MYGKRQYDPAPATYYRTDRISTVNGQYFFATREGTLEGPFFTRF
                     DAQQGASRYSRLHSSKAPPSPRRH"
     gene            complement(26190..27677)
                     /gene="pap"
                     /locus_tag="VIO83_02460"
     CDS             complement(26190..27677)
                     /gene="pap"
                     /locus_tag="VIO83_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HEY8330177.1"
                     /translation="MFESAEIGHQIDKETYDAAVPALREALLEAQFELRQQARFPVII
                     LLNGIEGAGKGETVKLLNEWMDPRLIQVETFDQQTDEELARPPFWRYWRRLPPKGRTG
                     VFFGNWYSQMLQGRVHQRIDDAQLDQAIGTTVRFERMLCDEGALIFKFWLHLSKRQMK
                     ARLKTLQDDPLHSWRISSLDWQQSKTFDDFVRYGERVLRRTSRDYAPWYVVEGGDERY
                     RSLTVGRILLEGLQAALRLKERPRRQPHAAPVAASIDNRGLVDSLDMTQTLDKDSYQQ
                     QLVAEQARLARLLRDKRMRRHALLAVFEGNDAAGKGGAIRRVTGALDPRQYHIVPVAA
                     PTEEEQAQPYLWRFWRHMPARGRFTMFDRSWYGRVLVERVEGYCSQADWLRAYSEIND
                     FEEQLTDAGVVLVKFWMAIDQQTQLERFQEREKTAFKRFKITEDDWRNCDKWTQYVDA
                     VNDMVDRTSTEIAPWTLIEANDKRFARVKVLRTLNEALEAAFARK"
     gene            27805..>28563
                     /gene="mnmD"
                     /locus_tag="VIO83_02465"
     CDS             27805..>28563
                     /gene="mnmD"
                     /locus_tag="VIO83_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HEY8330178.1"
                     /translation="MTEQAHAQIDWDEQGQPLSRTFGDVYFSRTSGVEESRYVFLAHN
                     HLAERFAALPAGGRLVIGETGFGTGLNFLCAWQLFEQTAPDTAHLHFVSAEKYPLTRS
                     DLERALALWPELAPQAGQLLAQYMAVHEGFQRFVFAGGRVVLTLLVGDVLDTLPQLDA
                     RIEAWFLDGFSPAKNPQMWTPELFAQLARLSAPGATLATFASTGFVRRGLIEAGFAMA
                     RLKGFEHKWEMLAGAFLGAPPAGGKPWFARPPRPS"
BASE COUNT         4819 a         9832 c         9293 g         4619 t
ORIGIN      
        1 gacgtcaacg cctggctgcg cgccctgctg ccgggctgat cccctcttgc cattcaggcc
       61 ggcagggcct gccgctcctg cgccaggcgg gcagccgcct gctccgctgg cagcgggcgg
      121 ccgcacagat agccctgcac cgcgtcgcaa cccagcccgc gcaacagttg cagctgcccc
      181 gcgctctcca cgccttcggc aatcacctgc agatccagcc cctggcccat ggcgatgatc
      241 gtggcgacga tcttgtcggc gcgctcgtcc tggcccaggc cgtcaatgaa gctcttgtcg
      301 atcttcagct tgtcgagagg gaagcgactg agataggcca gcgacgaata gccggtgccg
      361 aaatcgtcca ccgccagacg cacgcccagc gccttgagct cactcagacg cgcctgggtc
      421 gtcaggtcct gcgccatcaa cgcgccctcg gtgatttcca gctcgagcag cgcccccggc
      481 aggccgctgt gctccagcgc ccggcggacc agctcaacca catccagatg ctggaactgg
      541 tgcggcgaca ggttgacgct gaccggcacg tcgaacaagc ccgcctcgtg ccagcgcagg
      601 gcctggcgac aggcctcgtg cagcacccag tcgccgagcg ccaggatcag cccggtttcc
      661 tcgagcaaag ggatgaacac ccccggcgag accggctcgc cgctctccgg ctgccagcgc
      721 accagcgcct cgaagccgag tatccgtccg ctggcgacat ccacctgcgg ctggtagtac
      781 agcacgaact cctgggcggt gatcgctcga tgcaggcggc tttccagagc ctgacggttc
      841 tccgcggcgc gggtcagctc cttggtgtgg aaatggtagc tgttgcgccc gttctccttg
      901 gcctgataca tggccacatc cgcacagcgg atcagctcgg ccatttcctg gccgtcgtcg
      961 gggaacaggc tgatgccgat gctggcgcca acgaacagct catggccact gccggcgcgg
     1021 aacggctgct gcaggaggtt gatcagatcc tgcgccaccg tcgccgcctc ttcgggatgg
     1081 tggatggact ccagcaccag caggaactcg tcgccgccca ggcgggcgaa catgtcctcg
     1141 gcgcggatgc gctggcgcag acggcgggcg atcaacacca gcagctcgtc cccgaccgaa
     1201 tgaccgagac tgtcgttgac ccgcttgaaa ccatccagat cgaggaacag caccgccacc
     1261 ttgcaatgca ggcggcgggc ccgctcgatg gcgccattga ggtgcgactg caccagcagg
     1321 cgattgggca ggccggtcag cgggtcgtag tgcgccaggc gctcgagctg ggcctcggac
     1381 tccttgagct gggaaatatc gctgaacacc gcgacatagt tggtcagctt gcccttgtcg
     1441 tcacgtaccg cgctgatgct gtgccattgc gggaacacct cgccgctcct gcgccggttc
     1501 cacacctcgc cctgccagtg cccctcgtcg agcagcgccg cccacatgtc gcggtagaat
     1561 tccttgctct gtcgaccgga gctgacaatg ctggggttgc acccgagcac ctcgtgctcc
     1621 tcgtagccgg tgatgtcgca gaacgcgcgg ttgaccgcga tgatccgcgg cgaggtgtcg
     1681 gtgatcacca ccccctcacg ggcattggtg aacaccgccg cggcatgccg cagcttctcg
     1741 tccgtcgcct tgcgcgccga gatgtcgatc agcagcccct cgagatactg cacctcgccg
     1801 gccgtgtcgc ggtgcagccg cgagtagtcc tcgatccaga tatagctgcc gactccggta
     1861 cggatgcggt attcgaggac gaactccgga cagcgctcgt gaatgcagcg ctccacctcg
     1921 cccagcacca gcgccagatc gtccggatgg atcagggtgg tgaaccagcg cgatccgtca
     1981 agcaactgct cggcactgta gccccagcgc tcgacgttgt ccgaaacgta ctgcaccggc
     2041 cagccgttct cgagcttgcg gcgccacagc accatagggc tggccaccac ggcatccacc
     2101 accgcctgaa tggtttcgcg gtcccggtag cgctggctga catccagcag cgtgccgatg
     2161 atcgccggca ggccatccag ctcgatgcgc gtgccgaatg cctcggccat gaactcccgg
     2221 ccgtcacggg tcagggcgcg gaactcgaag tgcaccgaag cccgttcgcc gctctcgcaa
     2281 cgggctagca tgctgccgac ctcggcgcga ctcccctcgc cgaccagatc cagcagcgcc
     2341 cgcgccacca gatcgtccgc ctggtagccg aaccattcgg ccatgcgcgg attgacgtac
     2401 tcgaagtgcc cgtcgcgcaa cacgtagacg cccaccagcg agcgctcgac caggccgcgg
     2461 aagcgcgcct ccttggccat cagttcggcg ccggcgtcca gctcggcctg catgtcgaag
     2521 cagctgccga tgtagccgtg gaaacggcca tcgtgcccga ccagcggctc gccgtgcagc
     2581 tgcatccagt ggaagtggcc cgtccgcgag cgcaggcgca ggcgcgacga gaagcccttg
     2641 ccgaaaccat cgccgccggc cctcagcagg ggctcgagca gatgacggtc ctcctcatgg
     2701 accgcctgcc accagccctc cagcaggctg tccgtccggc tcatgccggt gaactgcagc
     2761 caggcggcat tcacgtacac acaggtgcgc tcggcatcaa cccgccagac cgggctgggg
     2821 atgcagttga gcagatattc gttgtaatcc cgcgccgtca ccacccgctg catcagcgcg
     2881 gcgaactcgg cagccagctg gcccacctcg tcgttgccca gcagcggtag cgtgcccagt
     2941 cgcccgctgc tcatgcgccg aacatcctgc agcaactcct cgatgcggtc ggtgagcagg
     3001 cgtttgagca gccaggccaa cagcagggcc agcagcagca acggcaacag cagcaggagc
     3061 aactgcttgc cggtgcgcga ccaggcatgg gcgcgcagcg tgtcggtacg catggccagc
     3121 accaccatgt ccggtacgcc ttgccccgcc agcccggtgc tcgtccccag cgggtaaccg
     3181 ccgaggagca ggtcgccctc ctccagccag tgcaggtggc tcccctccag gccggcccgc
     3241 tcacccagca ggcgctcgat ggccgggaca ctcagcggca tacgcgtgac accctgcggc
     3301 agactgagcg agcgcatccg cccgccactg tccagcaccg cggcgtagac cagcgccgga
     3361 tggttgagat gctcagcgaa cagatggccg agctgatcga tcgaagcgga accgttcagc
     3421 tccaggccgc cggccaggaa atggccgatg aaatcggtcg tggtttccag ctcgctcatc
     3481 gcctcctgca gcgaatggcg atgcaccgac accagctgat acacggacac cagcaggccg
     3541 aaaccgagaa aggcgctcag cagcaagccg cgcagggtga ataaccgatt catccgcaat
     3601 cactcaagag acagcagttt cgccaggggt aacgaggaca acccaccctc gagcctgagc
     3661 ccggagcagt actgccaggc aagggagccg gcggtttgag gagcagcatg gaccgcccca
     3721 tagggcgcca tgacaacagc ttactgtgca tggacctgca gcagaaatgc aaaagggtta
     3781 cgccagaaga cgtaaccctt tgctgtgcat caacaatatg gtgcgcgagg ggggattcga
     3841 acccccgacc taccgcttag gaggcggtcg ctctatccag ctgagctaca ggcgcgctgg
     3901 gtcggcgatg ttaaacgagg atctgcggct tgtcatgccc ctgcacgact tctctgccgc
     3961 agccggacag cgcgcagatg tagaaccatt cgcaattccg ccgccgacaa ctggccatga
     4021 tcgccggcag cccgcacact gcccacagca gcaatcgcca ggaagcacct gcgcaccgac
     4081 agaaaacccg gaccaggaga acaccgctga acggacacgc caaagcgtgg tcataccgtt
     4141 gccacattta tatatcgatc tgcataccct gataaatcgg tcatcgagag cccatgaaac
     4201 agagccccgc ctcccgctcc agccggactg ccgacaagtt cgtcgtgcgc ctgccggaag
     4261 gcatgcgttc gcgcatcgcc gaagttgccc gccactacca tcgcagcatg aactccgaga
     4321 tcatcgcgcg cctggaaaag accctgctcg ccgaaggcaa cctgcctgac gctgccgcca
     4381 atgcgccggt ccatggcgaa ctgcatcccc acgagcatca gctgctgcgc cactttcgcc
     4441 agctgcccca gccgcagcag agcgcactga tcgccctgct cggcagcagc cccggcactc
     4501 agaaaagctg aacaacgccc aatgaaaagc cgcctcgaaa ggcggctttt atctatgaac
     4561 agcagcccag cggcagctac agcaggaaca gggtcgccaa gccgagaaag atgaagaagc
     4621 cgccactgtc ggtgaccgcc gtaatcatca cactggcgcc catcgccgga tcgcgcccga
     4681 aacgcgccag ggtcatcgga atcagcacgc ccatcagcgc ggccagcagc aggttgaggg
     4741 tcatcgcgcc cgtcatgacc gcgcccagcg accagctgcc atacagatac caggccacgg
     4801 agccgatcac cccgccccac accacaccgt tgagcagacc aacgcccagc tccttgcgca
     4861 gcagacgcat ggtgttgccg gtacccacct gatcaagcgc catcgcgcgc acgatcatgg
     4921 tgatggtctg attgccggag ttgccgccga tacccgcgac gatgggcatc agcgccgcca
     4981 gtgccaccag cttctcgatg gagccctcga acagaccgat cacccgcgag gcgacgaagg
     5041 cggtgatcag attgagcgcc agccaggccc agcggttgcg caccgacttc cacaccgagg
     5101 cgaagatgtc ttcctcctcg cgcagaccgg ccatgttcag cacttcgctt tcgctttcct
     5161 cgcggatcag gtcgaccatc tcgtcgatgg tcaggcggcc gatcagcttg ccgctcttgt
     5221 ccaccaccgg cgcggaaatc aggtcatagc gctcgaaggc ctgggccgcc tcgtaggcgt
     5281 cctcgtcagg ctggaagccg accggatcgg tggccatcac ttcggccacc ttcttgtccg
     5341 ggtcgttgac cagcagccgc ttgatcggca gcacgccctg cagcatgccg tcgtaatcga
     5401 ccacgaacag cttgtcggta tgccccggca actccttgag ccggcgcagg taacgcagca
     5461 ccacctcgag ggtcacatcg tcacggatgg tgaccatctc gaagtccatc agcgcaccga
     5521 cctgatcctc ctcgtaggac agcgccgaac gcactcgctc gcgctgctgc gcgtcgagcg
     5581 tctccatcag ttcgtggacc acatcgcgcg gcagttccgg cgccagatcg gccagttcgt
     5641 cggcatccag gtcgcgggtg gcggcaagaa tctcgtgatc gtccatgtcg gcgatcaggg
     5701 tttcgcgcac cgcatcggac acttcgagga ggatgtcgcc gtcgcgctcg gccttgacca
     5761 gctgccagac ggtcaggcga tcatccagag gcagggcttc gaggatatgg gcgatgtcgg
     5821 ccgggtgcag gtcgtcgagc ttgcgctgca gttcggccag attctggcgg tgcaccagat
     5881 tttccaccaa ctcgtggtgg ccgccttcct ggcggtgggc cagttgctcg accagtcgct
     5941 gccggtgcag caggtcgatc acttgagcca ggcgatcctg caggctttcc tgcggctttt
     6001 tcgcttcagc ttcgctcatg atggcgcact ccctgtcccc cagcggcagg acggcgcacc
     6061 gagggtgatc aatctttcaa gatgctgacg aacaatcgag gttcggtagg tctactgggt
     6121 aagtccatga ctggatgtcc acgcaccccg ctgggggcag ataagcgcaa tggtagcatt
     6181 cgctcacccc gccacaggca aaaaatctcg acctgcagcg gagtccgtcg cctgcaccgg
     6241 cgagcgttgc gggcactcgc ggtgcatcgc caccgggccg aagtccgatg gcgaatgggc
     6301 aaaaaaaaag gcctgcatga ctgcaggcct ttttcgatat ggtgcactca ggaggattcg
     6361 aacctccgac cgcccggttc gtagccgggt actctatcca gctgagctat gagtgcatct
     6421 gtgatgttta gccagttcac ctctggattg aactgacagc cttggctgac agctcgaata
     6481 tggtgcactc aggaggattc gaacctccga ccgcccggtt cgtagccggg tactctatcc
     6541 agctgagcta tgagtgcact tgacggtgcg cattataggc tctgtcacgc gacagtcaag
     6601 atgtttttct ttaattttca atcacttacg tgatcgaaca acatctctac acctggtaat
     6661 gcaaaataat ggcggagaag gggggattcg aacccccgac acccttttga ggtgtactcc
     6721 cttagcaggg gagcgccttc ggccactcgg ccacctctcc gcaacacggg gcgtactata
     6781 cccaagcaaa ccccgtttgc aaagccctga ttcgataaaa atcgaaaaag aatcaatggc
     6841 ttggttcttc gtccttctcc ttctggatgc gctgatagat ttcttcgcga tgcacggcga
     6901 cttccttcgg ggcattgaca ccaatgcgca cctgattgcc cttcacgccc agcacggtca
     6961 cggtcacatc gtcaccgacc atcaaggtct ctcctacccg gcgagtcaga attagcattc
     7021 catttctcct tacgtctgtt tcaggacagc tgtctgcaag ataaaaaaaa acggtcacac
     7081 agccgcaggc attaaacctg ctggctatcg cccaagtatt gaccagcccc gccagaataa
     7141 aaggctgccg ggcggccaaa aacgacaaaa ggcgcggaaa cccgcgcctt ttgctcagtg
     7201 aacgtcactc accctgtcgg gccggagcgt ccagatcgaa agcggtatgc agggcgcgca
     7261 cggccagctc gagatacttc tcctcgacga ctacgctgac cttgatttcc gaggtggaga
     7321 tcatctggat attgatgttc tccttggcca gggcttcgaa catgcggctg gccacgccgg
     7381 cgtgggagcg catgccgaca ccgacgatgg acaccttggc gatctcggta tcaccggaca
     7441 cttcgcgcgc gcccatcgcg gcggcggtct gtttcagcac acccagcgcc ttgtcgtagt
     7501 cgttgcggtg caccgtgaag gtgaagtcgg tggtgttatc gtgcgccacg ttctgcacga
     7561 tcatgtccac ttccacgttg gcggcactga tcgggccgag gatcttgaac gccacgccgg
     7621 gggtatccgg gacgccgcgg atggtcagct tggcttcgtc gcgattgaag gcgatgccgg
     7681 agatgatcgg ctgttccatg gattcctctt cgtcgatggt aatgagggtg cccggaccct
     7741 cctggaagct gtgcagcacg cgcagcggaa cgttgtactt gccggcgaac tcgaccgaac
     7801 ggatctgcag caccttggag ccgaggctgg ccatttccag catctcctcg aaggtgattt
     7861 tctccagccg gcgggcacgc tgcaccaccc gcgggtcggt ggtgtagacg ccgtcgacgt
     7921 cggtgtagat ctggcactcg tcggccttca gcgccgccgc cagggccacg ccggtggtat
     7981 cggagccgcc gcgaccgagg gtggtgatgg cccccgtctc gtcgacgccc tggaagccgg
     8041 cgaccaccac cacgcgaccg gccttcagct cggcacgcag gcgctggtcg tcgatctcga
     8101 ggatgcgcgc cttggtgtgg gcactgtcgg tgaggatgcg cacctggttg ccggtgaagg
     8161 acaccgccgg cacgccgcgc tggatcagcg ccatggccag cagaccgatg gtgacctgct
     8221 cgccggtgga aatgatcgca tccagctcgc gcggatccgg ctgcggcatg atctgcttgg
     8281 ccaggtcgat cagacggttg gtctcgccgc tcatggccga caccacgacc acgatgtcat
     8341 cgcccgcctc gcggaacttc ttcaccttct cggccacctg cgcgatgcgc tcgacgctgc
     8401 cgaccgaggt gccgccgaat ttctgtacga tcaatgccat ttcgctgccg cctaaccgta
     8461 aaaaagtcgc ccattaaacc tgtcaggtct gtacctgcca agaacccgcc gggcgcgggg
     8521 cgggctccat ggggagaggt cagaacgcgg cggcgacgaa cggctcgacc agcgccagcg
     8581 cctcgtccag tttgccggcg tccacgccgc ccccctgggc catgtccggg cgaccgccgc
     8641 ccttgccgcc gaccacggca gccgcctgct tcatcagctc gcccgccttg agcttggcgg
     8701 tcaggtcctg ggtcactccg gccaccagca ccaccttgcc gtcgaactcg ccgcccagca
     8761 gcaccacggc gcgacccagc ttgttcttca gctggtcgac cagcgccagc agggccttgc
     8821 catctaggcc atcgagacgc gtggccagca ccttgacccc ggccacctcg atggccgcgc
     8881 cggccaggtc gtcgccggcg gcgctggcgg ccttggcctt gagctgttcc agctcctttt
     8941 ccaactggcg gttgcgctcg agcaggccgc ccagcttgtc cagcaggttg tcgcggctgc
     9001 ccttgaccag cgcggcggcg tccttcagct gctcctcggc agcgttgagg tagtccagcg
     9061 cctgcgcgcc ggtcaccgcc tcgatgcggc gcacgccggc agccacaccg ccttcgctga
     9121 cgattttgaa caggccgatg tcgccggtac gcgaaacgtg ggtgccgccg cacagctcga
     9181 cggagaaacc gccgcccatg ctcagcacgc gcacgctgtc gccgtacttc tcgccgaaca
     9241 gcgccatggc gcccttggcc ttggcggtgt cgatgtcggt ttcctcggtc tcgacctcgc
     9301 tgttcttgcg gatctcggcg ttgaccagtt cctccagcgc cttgagctgc tcgggcttga
     9361 tcgcctcgaa gtggctgaag tcgaagcgca gacgctggct gtcgaccagc gaacccttct
     9421 gaccgacgtg ctcgccgagg atctggcgca gcgcggcgtg cagcaggtgg gtggccgagt
     9481 ggttgagcgc ggtggcgccg cgcaccgtgg catcgaccac cgcctcgacc tgatcgccga
     9541 cgctcaggct gccggcatcg aggatgccgt ggtgcaggaa ggcgccgccg gccttgcggg
     9601 tgtcgcgcac gtcgaagcgc aggccattgg cttccagata gccgcagtcg ccggcctggc
     9661 cgccggactc ggcgtagaac ggcgtctggt cgaggaccac cacgccctcc tcgccggcgt
     9721 tcaggctgtc gacgttcatg ccttccttga acagcgccag cacgcggcca gcgccgcggg
     9781 tgcctgcgta gccggtgaag cgggtctcgc cttccacctt gatcaggctg ttgtagtcga
     9841 gatcgaaggc gctggccgag cgcgcgcgct cgcgctgggc ctgcatctcg cgctcgaagc
     9901 cttcctcgtc gatggagagc tcgcgctcgc gggcgatatc ggcggtcagg tcgaccggga
     9961 agccgtaggt gtcgtacagc ttgaacacca cgtcgccggg aatgatcttg ccctgcagtt
    10021 cggccaggtc ctgctcgagg atcttcaggc cctgctccag ggtcttggcg aactgctcct
    10081 cctcggcctt gaggacacgc tcgatgtgcg cctgctgctg gcgcagctcc ggataggcct
    10141 cacccatctc agcggccaac gcggcgacga tcttgtggaa gaagctgccc ttggcgccca
    10201 gcttgttgcc gtggcggcag gcgcgacgca cgatgcggcg cagcacgtag ccgcggccct
    10261 cgttggacgg ggtcacgccg tcggcgacca ggaagccgca ggagcggata tggtcggcga
    10321 ccaccttgag cgaggcctgg ccttcgttgg cgcagccgat ggcggcggcg gaggcgttga
    10381 gcaggctctg gaacaggtcg atctcgtagt tcgagttgac gtgctgcagc acggcgctga
    10441 tgcgctccag gcccatgccg gtgtccacgc tcggcgccgg cagcgggtgc agcacgccgt
    10501 ccgcggtgcg gttgaactgc atgaacacgt tgttccagat ctcgatgtag cggtcgccgt
    10561 cctcttccgg gctgccgggc gggccgcccc agatgtgctc gccgtggtcg aagaagatct
    10621 cggtgcacgg gccgcacggg ccggtgtcgc ccatcgccca gaagttgtcg gaggcgtagg
    10681 gcgcgccctt gttgtcgccg atgcgcacca tgcgcgcggc cggcacgccg acgtccttgg
    10741 tccagatgtc gtaggcctcg tcgtcgctgg cgtagacggt gacccacagc ttgtcggccg
    10801 gcaggttcag ccacttgggc gaggtgagga attcccaggc gtagctgatg gcgtcgcgct
    10861 tgaagtagtc gccgaagctg aagttgccca gcatctcgaa gaaagtgtgg tggcgcgcgg
    10921 tgtatccgac gttctccaga tcgttgtgct tgccgccggc gcgcacgcac ttctggctgg
    10981 tggtggcgcg ggtgtaggcg cgcttctcca ggccgaggaa gcagtccttg aactggttca
    11041 tgccggcgtt ggtgaacagc agggtcggat cgttcgccgg gatcagcgaa ctggaggcca
    11101 cgcgggtatg ccccttctct tcgaagaagc ggaggaaggc ttcacggatt tctgcgcttt
    11161 tcatggattg ttctcggaaa actgggccgc agcggcaatt gcaagagtgc cgcgcgtagt
    11221 gccggcaaag acgggcatta tataaacaac tggcccccgc ttgcatcgcc gggcgcacta
    11281 ttagcgctat tagagaacgc ggatgagccg gcgcgggcac acgactcagg agcagacctc
    11341 gtcgacagcg accggcacca cgatcagccc ggcgcactga ccgctggcca ccctgggact
    11401 ggggaagacg atgcgcgccc cctgctcctc caccagccag cgccgcccct cggcatcctc
    11461 ggccagcaga ctgcccggca gcagctcggt gaaactggcc acgtcttcgc ccagatgcag
    11521 gtgaaaggcg tcgctgtgct tgatcacctc gcgcgcgatg cgaaacagcg ggagggccgc
    11581 cggatcgcca tccatggccg gctcgcgacc ctcgagcagc ccccgcagcc aggcttccag
    11641 ccggctcagc tcgacctcgc cgccctgccc cagcggccgc gcctggccca gttcgaaggt
    11701 gaaggcctcg gccgcgaagc gctcgctggt gaaggcgctg aaggtcgccg caggctcgct
    11761 ctgcagcagc agggcctgca ggccggccgc ctgcaggccc gccagctgac cgcgcgaaag
    11821 aggctgcgcg ctgcgcgggc gcagggcgaa gcgctcgatc cgcgaagcgc gaatggccgc
    11881 atgcaggtcg taatgcatgc gccggcgccc cggcgggctg aagaacaact cgacctgctg
    11941 ctccagctca acggcacgca gggcgtccgc gccgctgacc gacggctcgc caccgccgaa
    12001 caaggcattg aggtcgtatt cgacatagcg ctggccacgg cgcagtgccg ccggattgcc
    12061 cagcaccacc agcagccgca cgaccggctg cagccggcag gaagcgatgt cgtgcagcag
    12121 acgctcgagc agttcgaccg gcgcggtctc gtcgccatga atgccggccg acaacagcag
    12181 gtcgaggccc gtgtcgcgcc ccgctggcgg gtcgatcagc agcacccctt cggccttcca
    12241 ctgcaggcgg gcgccggaag cacagacctg gatcttctcc gacggctcgc gcccggccag
    12301 agtcagctca agcagtttgc ccagagcaag catggcaggc cctccctacg accggcgacc
    12361 atgcctccag catagccgcc aggcagtcgc cgcggcgtca gtgatgatcg cagccgcagt
    12421 cggcgtcatg ctcgtggtcg tgctcatggg cctcggcggc ctccagctcg accgacagcg
    12481 acagctggtt gaccgcctgc gggcgcacca ccagcagcgg aatctcggtt tcgccttcca
    12541 ccgattcggc gtagatggtc acgcccccca tgccgtcggc ctccagccac agctcgcggc
    12601 cttcgaggcg cacgccgatg cgcgcgctgt gggtttcccg gcgttcgccg tgggcatctt
    12661 cgaggatcag gggcaggctt tcgctcatgg catttctcgc tcagacagac tgaaaaggat
    12721 aaagggaacc cagcttaagg ccttgcgccg ccgcgtccag cagcccgcgc cggctcgccg
    12781 cccggcggcc aagcggacga cactcagcgc cagccgacct gtgccgacag cggcaccgcg
    12841 cgcacctggg caccggcaga caggcgcagg cgcttggcgg tggcggcatc caccaccagg
    12901 gtgccggagg ccgggcgcgc cttgccgacg gtgatccggc agtcctcgcg cttgcggttg
    12961 tggatgagga actgcggcgc ctcctcgccc ggcgtgccga tcgccagcac cagggcctgg
    13021 ctgtcgcgca ccgcgcggat gctgcgggtc tcgcactcca gcgccgggcc gccgtcgaag
    13081 atgtcgatgt agccctggta ggtaaaaccc tcggcgcgca gcatggtcag cgccggctcg
    13141 gtgtcgcggt gcacgcgccc gatcaccgcg cgcgcctcct cggagaggaa gcaggtgtac
    13201 agcgggaact tgggcatcag ctcggcgatg aaggccttgt tgccgacgcc ggtgaggtag
    13261 tcggcctggg agaactccat cttgaagaag tgccgaccga ggctttccca gaacggtgcg
    13321 cgggcgttat cgtcggaaat tccgcgcatc tcggcgatca ccttgtcacc gaacagctcg
    13381 gggaattcgg cgatgaacag gaagcgcgcc ttggacaaca ggcgcccgct gagaccgctg
    13441 tgccggtagt tggcatccag gaacagcgag cagacctcgg tgttgccggt caggtcgttg
    13501 gccaggaaca gcgtggggtt ctgccggtgg atgcccagcg cgcgagaggc gctcacggtc
    13561 agcccgacgc ggaagttgta ccagggctcg cgcaggccca ccgcgccgtt catggcgctg
    13621 atgccgacca cctcgccggc atcggtctcc agcacgaaca ggtagtcggc gtcggcccgc
    13681 tcggcctcgc cgtggaagga tttttccgcc caggcgacgc gctgggccag gcgatcctcg
    13741 ctggccggca gcgaagtcag accggcaccg gtgctgcgcg ccatggcgat cagcgcgggc
    13801 aggtcggtgc tgcgaacggg acggacgatc atggtgcctc ctgtagacgg tcccgctgtg
    13861 ccggggccgg gccgcgctga ccggcgcggc atcggtggcg aaggccgcag cctccgcgat
    13921 ggattctgct cagcggccgg cgcgactcac aacgccacca ctcgcacgct gccgccctcg
    13981 ccgaccccca gcgcctcggc cagcgccggc gtgagggtca gcggctggcc gacttcccag
    14041 tccagctcga cgatcagcgc acggaaatcc tgcagctggt tgttggccac caggtaggcg
    14101 cgcccggact gcgccggcgc acccaggcgc accggcacca gacggctctg ggcgatggag
    14161 cgcagacccg cggtgcgcgc atgcacggtg ggtccgccgt cgaagatgtc gacgtagtgt
    14221 tcgctctcga agccttcgcg catcaggatg tcgaaggaca cctgcgagcg cgggtgcacc
    14281 tgccccatcg cctcctgggc gctgtcgggc agcagcggca cgtagatcgg gtagctcggc
    14341 atcagctcgg cgagcatggt gcgacccttc aggccacaca ggcgctcggc ctcggcgtag
    14401 ccgatggcaa ggaaattgcg gccgatggca tcccagaacg gcgcatcccc cttgtcgtcg
    14461 ctgtagccga ccacctcgac caccatcgac tcggcgaaac gctccgggtg gctggcgacg
    14521 aacagcagac ggccgcgcga gttgagttct gcccagggcg tgctctccag ctccggctgc
    14581 aggtagaagc tgatcagcag gctgttgccg gtcaggtcgt gacacatcga cagcacatgg
    14641 atcttgttgt ggatctgcag ctcgcgcgag gcgtgcacga aggtctcgtt gcggaagctg
    14701 tagaacggct cggaaaagcc ggcggtggcg acgatccccg agcagccgac cagcttgccg
    14761 ctggccgtgt cctcgaggac gaagaaatag ctctcctcgc cattgaagct cacctcggcg
    14821 gcgaacgagg cctccgaggc gcggatcttc tcggccagac gctcgcggtc gtccggcagc
    14881 gaggtgacac cgaccggact gtccgccgcc agccgctgga cttccggcag gtcgcccatc
    14941 cgggcggggc gcatgatcag catcggtgtc tcctcgtcgg gcagtggtcg ccggtcaggc
    15001 ggcggtcagg ctggccaggg cgcgctcgag acgggccatg ccctcgtcga tgtcggcctc
    15061 gctgatcacc aggctggggg cgaagcgcac cacgtccggg ccggcttgca ggatcatcag
    15121 atcctgctgc tgggcagcgg ccatcacctc gccggcgcgg cccttccagg cctcggtcag
    15181 caccgcgccg agcagcaggc ccatgccgcg cacctggctg aacaccccgt actgctcacc
    15241 gaggcgcagc agctcgcggg tgaagtgcgc gctgcgcgcg ccgacgccgg acagcacctc
    15301 gggagtattg acgatgctga tcaccgcctc gcccaccgcg caggccagcg ggttgccgcc
    15361 gtaggtggtg ccgtgcacgc cgacgccgaa gtgcttggcc accgcgtcgg tggtgagcat
    15421 ggcgctgatc gggaagccgc cacccaggct cttggcgctg gtgaggatgt ccggggtcac
    15481 gccgtagtgc atgtagccga acagctggcc ggtgcggccg acgccggtct gcacctcgtc
    15541 gaagatcagc agcgcgttgt gctcgtcgca cagcgcgcgc acgccttcca gataggcctg
    15601 atcggccggc agcacgccgc cctcgccctg gatcggctcc agcaccaccg cgcaggtgtt
    15661 ggcggagatc tgcgccttca gcgcttccag gtcgttgtac ggcacatggc tgatgccttc
    15721 gatctgcggg ccgaagccgg aagagtactt gggctggccg gcgacgctga cggtgaacag
    15781 ggtacgaccg tggaagctgt tgagcgcgga gataatctcg cacttctgcg ggccggcgac
    15841 ttcgtggcca tagcggcgcg ccagcttgaa cgcggcctcg ttggcctcgg cgccggagtt
    15901 ggcgaagaac acgcggtcgg cgaaggtggc ctcggtcagc agcttggcca ggcgcagggc
    15961 cggctcgttg gtgaacacgt tggacacgtg ccagagcttc tgcgcctgct cggtcagcgc
    16021 ctcgaccagc gccgggtggg catggccgag cacgttgacg gcgatgccgc cggtgaagtc
    16081 caccagctcg cggccgctct gatcccagac tcggctgccc tcgccacgca ccggaacgaa
    16141 ggcggcaggg gcgaaaacgg gaaccatcag ctggtcaaaa tcggcgcgtt gcaccggcgc
    16201 gtgcggaacg gacatcgaac tctcctgcct gggcggcaaa tcggattgaa cagaaaggga
    16261 ttgtaaagac aaagaccggc gcggcattgc aggcatgcga catcttcgta cagttgtcgc
    16321 cggcgcgatt gcacaaccgg atgcgccgac accctgccgg cggcgaaaag ggtgcagtgt
    16381 atagctcggc ccggccgctc gaaagtccgc cgtggcgcca gcggacgaat ccactcgccg
    16441 caggcccatg gcggcgtctc agccgtgctc gcccagcccg ccaccgacct cgcccgtcgc
    16501 cgcggcccgg ctcaggttgc ggtcctcgcg cggggtgacc ccgaagaagt tgcggtaggc
    16561 gctggagaag tgcgggccgg aagaaaagcc gcaggacagg ccaatctgga tgatcgaggt
    16621 actggtctgc tgcaacagct ggcgcgcgcg gttcaggcgc agctccaggt agaactggct
    16681 gggcacgcga ttgaggaact gcttgaagat ccgctccagc tggcggcgcg acacgcacac
    16741 atgctgggcg atcgcgtcgg tggtcagcgg ctcctcgatg ttcgcctcca tcagcagcac
    16801 cgcctgggtc accttcgggt gcgccgcgcc gaggcggttg cgcagcggca cccgctggcg
    16861 ctcgccgccc tcgcggatgc gctccagcac cagctcctcg gcaatcgcgc cggccagttc
    16921 ggcgccgtgg tcgcgggtca gcatggccag cagcatgtcc agcacggcca gaccgccaca
    16981 ggcggtcagg cgctcgcgat cccactcgaa cagatggttg gtggcgccga ccagagggaa
    17041 acgctcgctg aaatcgtcct gccagcgcca gtgcaacgcc acccggtggc catccagcag
    17101 gccgagctgg gccagcggat acaggccagc gcccaccgcg ccgagcaatc cgccgtggcg
    17161 cgccagctgg cgcaaggcgg tcgccagttc gggggccagc tccggtggct cctcggccag
    17221 caggaacagc cgctcgctac cttccagacg ctcctgccag ggctggccag gcagcgccca
    17281 gccgtcgccc tccacgggcg cggcctgcag gaagctgacc gtgtagagcg cctccggatg
    17341 cagacgcatc gccagctgca gggcttcgtc gatcagggtc agggtcagcg gacgcacgtc
    17401 cggccagtag agaaatgcga gatgtacggg gttcatggac ggcagtctgg ccggattgct
    17461 tgcgcaaggc taggcacgga atgtggagga cgcggcaatt taaccggaac cacgccgcca
    17521 cgcaccaaaa aggtgcataa atccactcaa aagtccacct tgccgcgccc gctcttgatc
    17581 accgcgcgct tgctcttgcc ctccaggcgg cgctgcttcg agccgaacgt cgggcgggtc
    17641 ggccggcgcg ccttctccac cttggccacg ctgcggatca gctcgaccag gcgctcgagg
    17701 gcgtcggccc gattctgctc ctgggtccgg tactgctggg ccttgaggac gatcaccccg
    17761 tcgccggtga tgcggctgtc gcgcagcgcc agcaggcgct ccttgtagaa ggccggcagc
    17821 gatgaggcgt ggatgtcgaa gcgcaggtgc acggcactgg aaaccttgtt gacgttctgc
    17881 ccgccggcgc cctgggcgcg gatggccgtc agctcgacct cgctgtccgg cagatggacg
    17941 ctgttggaaa tttccagcat ggggcatgac ctgtcagtgg cgactcgcag gatagcgccg
    18001 ggccaggcgc ctgtcccggc acaaacaaca acgccgcgcc cgccgcgaca cccttgcggg
    18061 tgcccggcgg aacgcggcgt tgtcaacgaa gcgctcccgc cagccatgcc ggcggcggga
    18121 gcgcttcacg gtacagacac tcagcccggc tggttcagct cgaggacctt ggcggtgcgc
    18181 gcacgaacgc gacgcagcag ctcggcatcc ttctgcaggg tctcctcgtc ggcctcgaag
    18241 atctgtccca ggcgctcctg ccaggcagcg ctgcggtact gctcggggaa gcagcgctcg
    18301 atcacttcca gcatgaccga caccgacacc gaggcgcccg gggaggcgcc cagcagcgcg
    18361 gcgagggagc catcggccgc gttgacgatc tcggtgccga actgcaggac cccgcccttc
    18421 ttggcgtcct tcttgatgat ctgcacgcgc tgaccggcga tctcgaggcg ccaatcttcc
    18481 ttcttcgccg ccgggaagaa cttgcgcagc tcttccagac gctggtcctc ggactgcagc
    18541 acctccttga tcaggtactt ggtcaggtcc atgttgtcgc gcgccacggc cagcatcgga
    18601 ccgatattgt tcgggcggat cgacagcggc aggtcgagga aggaaccctg cttgaggaac
    18661 ttggtggaga aaccggcgaa gggtccgaac agcagcgact tgccgccatc gaccacgcgg
    18721 gtatccaggt gcggcacgga catgggcggc gcaccgattt cggcctggct gtagaccttg
    18781 gcctggtgct gcttgacgat ttccgggttg tcgcaacgca gccactggcc gctgaccggg
    18841 aagccgccga agcccttgcc ctcttcgatg ccggacttct gcagcagggt cagggccgcg
    18901 ccgccggcgc cgaggaacac gaagcgggcg gtcacttcac ggctgccgcc acgcgccacg
    18961 tcgtcgacgg acaggcgcca gcggccgtcg gcctcgcgac gcaggtcggt gatcttctgg
    19021 ttgtaggcga ccttgcagcc ttcgctctgc tcgagatgct ggagcagctt cttggtcagc
    19081 gcaccgaagt tgacgtcggt accgcccatg gcacgcgtcg ccgccagcgg ctcgttggtg
    19141 gtgcggccct gcatcagcag cggcgcccac tggccgatgg tctgacgatc ctcggtgtac
    19201 tccaggtcgt cgctgaagca gtgatgcgcc ttcatggcgt cgaagcgctt gcgcaggaag
    19261 gacacgcctt tctcgccacg cacgaagctc atgtggggaa cggggctgat gaaatccttc
    19321 ggcgacttga agtagccctt ctccaccagg taggcccaca gctgacggga cacctcgaac
    19381 atcgtgttga tgctcaccgc cttggcgata cccaccgaac catccggacc ttccggcgtg
    19441 tagttcagtt cacagagtgc agcgtggccg gtccccgcgt tgttccacgg gaaagaactc
    19501 tcggcagccc cggattcgag ttgttccagg acttcgatct tcaagctcgg gtcgagctct
    19561 ttcagcagta ccgcgagggt tgcgctcatg acgcctgccc cgacgaatac cagatccggc
    19621 tgatcactac cagtttgcat cattcatgct cctgagacac aggtttcgca ttttacctcg
    19681 gatcgtcgac agtcgcgtac tgtagtccgt ccgacaatgg tactacacgc tcccggaata
    19741 ccccccgata agggggcagg cgaaggcggc gcaatgctaa cgatgtgcgc cgcgcagcgc
    19801 cagtgccgcc tgttgctggc ccgaaaaaag tcgcaggagc cggcaactgc gcggcaaccc
    19861 gtgccgcgcc gggacagccg acggcatccg gtaggggacg gcgggcagac actacagctc
    19921 cgcgggtcag cccccgcgca ggcggcgccc gagcgcgatt gtcgacactt cgccgctgcg
    19981 gccatcagag caccgaatcg aacagttcca gctgcggcgg ccccagatag atctcgcgcg
    20041 ggctcttgcc ggcgttggcg tgcgccttgc ggaacgattc ggagtgggtc cagtcctcga
    20101 agctctgcgc cgactcccac tcggagtagg aggagaacag cgtgtactcc tcggtctgcg
    20161 gccccttgag cagatggaag cgcaggaagc ccggcacctc gtcgaggtag ctttcgcgct
    20221 ggcgccagtg gtcgatgaag gtttcttcgt ggccgcggac aatcttgaag cggttcatgg
    20281 caaggaacat gcttttggct ccgggtgata aagcagtcgc cattctagcc cccgccccgg
    20341 cccgtgcgca tggctcatca atgctgcagg ttgccgtaca gccgggcata cagcccgccc
    20401 tcggcgatca gctcctggtg gccgccctcc tccgccacgc gcccgccatc gaacaccagc
    20461 acgcggtcgg cctgcttcac cgcggacagg cggtgggcga tgatcagcgt ggtccgcccc
    20521 tgcaggaagc gccccagcgc ctgatgcagg gcgtactcgg tggccgcatc cagtgccgag
    20581 gtggcttcgt cgaggatcac caccttgggc tcggccatga ccatgcgggc gatcgccagg
    20641 cgctggcgct ggccgccgga gaggcgcacg ccggagcggc cgaccacgct gtccaggccc
    20701 tggggcagcg cccgcacggt gtcggccagc tgggcgatct ccagggcgcg ccagcaggcg
    20761 ccatcgtcgc cctcgcggcc catgctcagg ttggcgcgca ccgtgtcgtt gaacaacgcc
    20821 ggatgctgca gcaccaccgc cacctgctcg cgcacccgct ccaggccaat ctcctcgagg
    20881 gtcgcgccgc cgaagcggat ggtgccggcc tgcggcgcat agaggccgag cagcaactgc
    20941 accagggtgc tcttgccgcc ccccgaggcg ccgacgatgg ccaccttctc gccgggcgca
    21001 atgcgcaggt cgaggccgtc gagcaccggc tcatcgcgat aggcgaagcg caggccctgc
    21061 acctcgatgc cgaccgtggt ctgcccggcg aagggatcgg cgccgccggg gtattgcggc
    21121 tcgtcggccc gagcgagcaa ctcgttgagc cggctcagcg cgccgccggc cgcatagtag
    21181 gcgtactgca ggccgagcag ctgctcgacc gggccgatca tgaaccacag gtagctgaac
    21241 accgccagca tctggccgat gctcaggtcg gagaacagca cggtgagcat ggccgcggcg
    21301 cggaacacgt cgatgccgaa ctggaacagc aggccgctgg cccgggtggc ggcgtcgctc
    21361 ttccacagcg aggccaccgc gtagtcgcgc acctcgcggg cgcgagtacc gaggcggccg
    21421 aggaagaagc cctggcggtt gccggcgcgc agctcctgga tcgcctcgag ggtttcgccg
    21481 agcgcctggg tgaagcgcgc ggtgctgtcg ttttccagct tcttcaggtg cttgacccgc
    21541 ttgcccagct gcaccgtggc gtagatcacc agcgggttga acaacaggat cagcagcgcc
    21601 agctgccagt gcatccagat caggatcgcc gaggtgccgg ccagggtcag cagcgccacc
    21661 agcaagcggc tgagggtatc gccgacgaac ttgtcgaggg tatcgagatc ggtgaccaga
    21721 tgggcggtca ccgtgccgct gccgaggctc tcgtactcgg cgagggaaat gcgcctgagc
    21781 cgctcgatca ggcgcaggcg cagccggtag accacatcct tggacagccc ggcgaacagc
    21841 ctggcctgca gcacgttgaa cgccagcgag gcgccacgca gcagcagggt cgccagcagc
    21901 atcaggccga tatagcccgc cgcctgctgc caggcgacgg gcagcaggcg gttcatcacc
    21961 tgcagcgcgg cgtcgccctg gcccagcagc acctcgtcga ccagcagcgg cagcagcagc
    22021 ggaatcggca cgctgcacag ggtggccagc acggccacca gattggcgat cagcagttcg
    22081 cgacgatgcc ccagcgccag ccgacggatt tccgcccagc tcagccggtc gcggctgtcg
    22141 ccgccatcag gcataggcac ggcgctccag ccagcggccg agcagcggcg ccagcgtctc
    22201 cagcggctgg tagccgttgg tcagcagcgc cagctggccg ttgcgctcgg ccagcagggt
    22261 gggaaagccg gcgatgccga ggttttgcgc ccaggcgaaa tcggcctggg tggccgcgcg
    22321 ctgggccgcg ctgtcgaagg ccgcggcgaa ggcctggcgc ggcacgccga gctgttcggc
    22381 cagctcgacc aggaccgccg gacgggtgac atcgcgcccc tcgacataga acgcctgctg
    22441 aatcgcctgc accagcggcc agaccctgtc cgcggccagg ctgcgcagcg ccaccagggc
    22501 gcggctggcc ggctcggtgt cgtagacgaa gccttccggc aaggccccgt cgaaacggaa
    22561 agcctggccg gtgcgctcgg ccaccgcacg ccagtgcttg aggatgtagt cgcggctcgc
    22621 gccatccagc gcctgcgcag tgccgggacg caggccgccg gcgaccaggt gcaggctcac
    22681 gtcggcggac tgcgccaggg cagccagggc atcgaacacc ggggcgaagc cccagcacca
    22741 agaacacatc ggatccatca catagagcaa gcgggcgttc atccgggctc ctgttggccg
    22801 ggcagcggcc agtaaggtgc gccacgcgca ccggaaaggg gacgggccgg cgcgcggccc
    22861 accatcgcgc gtcagtcggc ctggcgcgga ttgcgaccga tcgggtgcgg ctggtgacgc
    22921 gcgcgggcca ggtcgatctg tttctgccgc tcgcgggcgc tggcgcgggt cttctcgctc
    22981 aggctgtccc agcagtgcgg acagctgatg cccgccacgt agtgctcgtt ctggcgatcc
    23041 gccaccgaca ccgggttgcg gcaggcatga cactggtcgt agtcaccctc actgagatcg
    23101 tggcgcacgg tgacccggtt gtcgaacacg aagcagtcgc cgcgccacag gctctgcgcc
    23161 tgcgggactt cctccagata cttgaggatg ccgcccttga ggtggaacac ctcggcgaag
    23221 ccctggccga gcatgtagct ggaggccttc tcgcaacgga tgccaccggt acagaacatc
    23281 gccaccttct tgtgcttcgc cgggtcgaag tgctgctgca cgtactcggg gaactcgcgg
    23341 aacgacttgg tctgcgggtc gatggcgcct gcgaaggtgc cgatggccac ctcgtagtcg
    23401 ttgcgcgtgt cgatcagcag cacctccggg tcggagatca gcgcattcca gtcctgcggc
    23461 tcgacatagg tgccgaccgc ctggctcgga tcgacgccgg gcacgcccag ggtgacgatc
    23521 tcctgcttga gcttgacctt ggtacggtag aacggctgct cgctgcaata cgattccttg
    23581 tggtcgatgt cgaccagccg cggatcggcg tgcagccagg ccagcagcgc gtcgatggcg
    23641 gcgcggctgc cggacacggt gccgttgatg ccttccgccg ccagcagcag ggtgcccttg
    23701 acgccgtttt ccagcatcgc cgccagcagc ggctcgcgca gggcgacgta atcctcgagg
    23761 gtgacgaact tgtacagcgc cgccaccacg atgttctgac tcatgacgct ctccagtggt
    23821 cgcccgcgca aggggcggac cggatcttga agggttccag aaacgacaac gccggcggga
    23881 gccggcgcgg cgcggattct agcaaaggcg ccggccgccg cggcacgcca gccgctcagt
    23941 gggagtgatg gtcatgcttg ctgccgccca tgcaggtcgg cgaatccggc gcgcctccct
    24001 gcgccgccca ctcctcgggg gtgtaggtat gcagcgccag cgcatggaaa ctgcccatca
    24061 gtgcgccgag actggcatag accttctggt ggcgcttgac cgcattcagg ccggcgaaca
    24121 gcgggctgac caccaccgcc ttgaagtggc tttcctgacc acggctgtgc atgtggctct
    24181 cgttgagcac ctcgaggtgc tgcggctcga gggcggccag cgcctgctgg atctgttcct
    24241 gcatcgacat cggtgttctc cgcaatggtt cgggcggccg cccgctaatg gagccattct
    24301 gcgcagaacc tccccgccgc tcaagcacag gactgtcgca gcggctggac ggacggcggt
    24361 ctgccctgcc cgccgcggcc tagactgagg aaaatcctgc ggagaccgtc cgatgagccg
    24421 taccgaaacc gacagcctgg gccccatcga ggtggcgacc gaggcctact ggggcgcgca
    24481 gacccagcgc tcgctgatca acttcgccat cggcagcgag cgcatgccgc tggcggtgat
    24541 ccacgccctg gcgctgatca agaaagccgc cgcgcgggtc aatgcgcgca gcggcgatct
    24601 gccggcggac atcgccgccc tgatcgagca ggccgccgac gaggtgctgg ccggccggca
    24661 cgacgaccag ttccccctgg tggtgtggca gaccggcagc ggcacgcaga gcaacatgaa
    24721 cgtcaacgag gtgatcgccg gacgcgccaa cgagctggcc ggccagggcc gcggcggcaa
    24781 gcagccggtg caccccaacg accacgtcaa ccgttcgcag agctccaacg actgcttccc
    24841 caccgccatg cacatcgccg tggtgctggc gctgcacgag cagctgctgc cggcgctgca
    24901 cgagctacag ggcggcctgc aggcacaggc cacgcggcat gccgcgctgg tcaagaccgg
    24961 ccgcacccac ctgatggacg ccacgccgat cacctttggc caggagctgt cggccttcgt
    25021 cgcccagctc gactacgccg accgggccct gcacgccgcc ctgccggcgg tctgcgagct
    25081 ggcccagggc ggcactgccg tgggcaccgg actgaatgcg ccggagggct tcgccgaggc
    25141 catggctcgc gagctgagcg agctggccga acagctgttc gtcagcgcgc ccaacaagtt
    25201 cgccgccctc gccggccacg agcccctggt cgccctctcc ggcgcactga agaccctggc
    25261 ggtggcgctg atgaagctgg ccaacgacct gcgcctgctc ggctccggcc cgcgcgccgg
    25321 gtttgccgag gtgcgcctgc cggccaacga gccgggcagc tcgatcatgc ccggcaaggt
    25381 caacccgacc cagtgcgagg cgctgtcgat gctcgcctgc caggtgctcg gcaacgacgc
    25441 cacggtgagc ttcgccgcca gccagggtca cctgcagctc aacgtgttca agccggtgat
    25501 cgtgcataac ctgctgcagt cgatccgcct gctcggcgac ggctgccgca acttcaacca
    25561 gcactgcatc gccggcctgg aaccggatgc cgcccgcatg gccgagcacc tggagcgcgg
    25621 cctgatgctg gtcaccgcgc tcaacccgca catcggctac gacaaggcgg cgcagatcgc
    25681 caagaaggcc tacgccgagg gcaccagcct gcgccaggcg gcgctggcgc tgggttatct
    25741 cagcaacgag gacttcgaca actgggtgcg cccggagcag atgctcgcgc ccggcgcgca
    25801 aggctgagcg tcaacgcgcg cgccggcgct gcagcagggg cggcccaaag cgaccggctc
    25861 acgccggccc tgctgcagcg tgggttgccg ctggtcgcgc gcgccgtccc tgtcgccggg
    25921 gggaactaga cttcagacgt ctatcctttt ttccggggga gaacgctcca tgtacggaaa
    25981 acgccagtac gatccggcac ccgccaccta ctaccgtacc gatcgcatca gcacggtcaa
    26041 tggccagtac ttcttcgcca cccgcgaagg caccctggaa ggaccgttct tcacccgctt
    26101 cgatgcccag cagggtgcca gtcgctacag ccggctgcac tccagcaagg cgccaccgtc
    26161 gccacgacgc cactgagcca gcggcttgat cacttgcggg cgaaggccgc ctccagcgcc
    26221 tcgttgaggg tgcgcagcac cttgacccgg gcgaagcgct tgtcgttggc ctcgatcagc
    26281 gtccaggggg cgatttcggt actggtgcgg tcgaccatgt cgttcaccgc atcgacgtat
    26341 tgcgtccact tgtcgcagtt gcgccagtcg tcctcggtga tcttgaagcg cttgaacgcg
    26401 gtcttctcgc gctcctggaa gcgctccagc tgggtctgct ggtcgatggc catccagaac
    26461 ttcaccagca ccacgccggc atcggtgagc tgctcctcga agtcgttgat ctcgctgtag
    26521 gcacgcagcc agtcggcctg cgagcagtag ccctcgaccc gctccaccag cacccggccg
    26581 taccaggagc ggtcgaacat ggtgaagcgc ccgcgtgccg gcatgtgccg ccagaagcgc
    26641 cacaggtagg gctgggcctg ttcctcctcg gtcggcgccg ctaccggcac gatatggtac
    26701 tggcgcgggt ccagcgcccc ggtcacccgg cggatcgccc cgcccttgcc cgccgcgtcg
    26761 ttgccctcga acacggccag cagcgcatgg cggcgcatgc gcttgtcgcg cagcaaccgg
    26821 gccagacgcg cctgctcggc caccagctgc tgctgatagc tgtccttgtc cagggtctgg
    26881 gtcatgtcca ggctgtcgac tagaccgcga ttgtcgatgc tggccgccac cggcgcggca
    26941 tggggctggc gcctggggcg ctccttgagg cgcaacgccg cctgcaggcc ctcgagcagg
    27001 atgcgcccga cggtgaggct gcgatagcgc tcgtcgccgc cctcgaccac gtaccagggc
    27061 gcatagtcgc ggctggtacg ccgcagcacc cgctcgccgt agcgcacgaa gtcgtcgaag
    27121 gtcttcgact gctgccagtc cagcgaactg atccgccaac tgtgcagcgg atcgtcctgc
    27181 agggtcttga ggcgggcctt catctgccgc ttcgacaggt gcagccagaa cttgaagatc
    27241 agcgcgccct cgtcgcagag catgcgctcg aagcgcacgg tagtgccgat ggcttggtcg
    27301 agctgggcat cgtcgatgcg ctggtgcacc cgcccctgca gcatctggct gtaccagttg
    27361 ccgaagaaca cgccggtacg ccccttgggc ggcaggcgcc gccagtagcg ccagaacggc
    27421 ggacgcgcca gctcctcgtc ggtctgctga tcgaaggtct cgacctggat caggcgcggg
    27481 tccatccatt cgttgagcag cttgaccgtc tcgcccttgc cggccccctc gatgccgttg
    27541 agcaggatga tgaccggaaa gcgcgcctgc tggcgcagct cgaactgcgc ctcgagcagc
    27601 gcctcgcgta acgcgggcac ggcggcgtcg taggtttcct tgtcgatctg gtggccgatt
    27661 tcggcggatt cgaacatgcg ggctctccct ggcgatagct gcagcctagc cgatcgccgg
    27721 gggtggcacg caagggtttt gcctgccggc agcggtcgat cggctagaat ggccgcccct
    27781 tccccctttg ccgcccccgc accgatgacc gaacaagcgc acgcccagat cgactgggac
    27841 gaacagggcc agccgctgtc ccgcaccttt ggcgacgtgt acttctcgcg cacctcgggc
    27901 gtggaggaaa gccgctacgt gttcctcgcc cacaaccacc tggccgagcg cttcgccgcc
    27961 ctgcccgccg gcgggcgcct ggtgatcggc gagaccggct tcggcaccgg gctgaacttc
    28021 ctctgtgcct ggcagctgtt cgagcagacg gcgccggaca ctgcccacct gcacttcgtc
    28081 agcgcggaga agtatccgct gacccgcagc gacctcgaac gcgccctggc cctgtggccg
    28141 gaactcgcgc cgcaggccgg acagctgctg gcgcagtaca tggcggtgca cgagggcttc
    28201 cagcgcttcg tcttcgccgg cgggcgagtg gtcctcaccc tgctggtggg ggacgtgctc
    28261 gacaccctgc cgcaactgga tgcacgcatc gaggcttggt tcctcgacgg cttttcgccg
    28321 gcgaagaacc cgcagatgtg gacgcccgaa ctgttcgccc agctcgcccg cctgtcggcg
    28381 cccggcgcga ccctcgccac cttcgccagt accggctttg tccgccgcgg actgatcgag
    28441 gccggcttcg ccatggcccg cctcaagggc ttcgaacaca agtgggagat gctcgccggc
    28501 gccttcctcg gcgcgccgcc ggccggcggc aaaccctggt tcgcccgccc gccgcggccc
    28561 agc
//
LOCUS       DATCUN010000064         4836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_152860, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000064 DATCUN010000000
VERSION     DATCUN010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152860"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2796
                     /locus_tag="VIO83_02470"
     CDS             <1..2796
                     /locus_tag="VIO83_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HEY8330179.1"
                     /translation="QAGAPEPGAAVQGRRPATVADEQALYDVAPSFAELLPWVEFLPQ
                     SKSMLLEDGQSVAAFYELVPLGTEGREPGWLAHARDALENALQDSFDELDENPWVLQL
                     YAQDEPSFDQYMQTLRDYVQPRARDTAFTEFYLRFFSHHLRAVAKPGGLFEDTVVTRL
                     RWRGQTRRVRMVIYRRATGQASRRGQTPEQMLNIVCDRLCGGLANAGIQARRMVAADV
                     HDWLLRWFNPRPAMLGPSAEDRERFYALARYPDSTEESEDGEIELASGRDFSQRLFFG
                     QPRSDVEHGTWYFDGMPHRVLITDRLRMPPGTGHLTGETRKGDAINTLFDQMPEDTLM
                     CLTMVATPQDVLEADLNHLAKKAVGETLASEQTLKDVQEARSLIGSAHKLYRGTLAFY
                     LRGRDEAELDRRGLDLANVMLNAGLQPVREDDEVAPLNSYLRWLPCCYNPGQDRRKWY
                     TQLMFAQHAANLSPVWGRAQGTGHPGITMFNRGGGPITFDPLNRLDRQMNAHLFLFGP
                     TGSGKSATLNNLLNQVTAIYRPRLFIVEAGNSFGLFSDFARRLGLTVNRVKLAPGSGI
                     SLAPFADARRLIETPSDVQTLDADALDEDLPPDASAMEADEQRDVLGELEITARLMIT
                     GGEDKEEARMTRADRSLIRQCILDAAEHCHGGGGSEKRTVLTRDVRNALRTRGQDPTL
                     PEMRRVRLLEMADAMDMFCQGTDGEMFDRDGTPWPEADITLVDLATYAREGYNAQLSI
                     AYISLISTVNNIAERDQYLGRPIINVTDEGHVITKNPLLAPYVVKITKMWRKLGAWFW
                     LATQNIDDLPRAAEPMLNMIEWWICLSMPPDEVEKIARFRELNASQKALMLSARKEAG
                     KFSEGVILSKSMEVLFRAVPPSLYLAMAMTEPEEKAERFQLMQRHGISELDAALRVAE
                     KIDRARGIEPLALDTLA"
     gene            2810..3550
                     /locus_tag="VIO83_02475"
     CDS             2810..3550
                     /locus_tag="VIO83_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY8330180.1"
                     /translation="MRMPSSARRHPRIGLLIVATIGVALMLLRVPHPATESMPPAAAG
                     SKAPKPAGPPWLYGRADARFTVVGYADLECPYCRAHFPALKRWIDAHPEVNWQWRHLP
                     LSMHEPAATVEARLAECAGEAGGHAAFWQAVAWLYANTRGDGQGLPEGLRYPALTPAM
                     QACLDSERPDAVIRAQAVEAAQQGIAATPTLLLRDRDTGKTLLLHGPMDGDALLSALD
                     LLAAGDTASAESSHPPDMPAGVAGDMPR"
     gene            3746..4240
                     /gene="radC"
                     /locus_tag="VIO83_02480"
     CDS             3746..4240
                     /gene="radC"
                     /locus_tag="VIO83_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY8330181.1"
                     /translation="MSLVIADSRPESLTLIAAQHEDWIIRQAITLLENRVFKAGPALG
                     SPAAVRDYLRLKLVAEPNEIFAVVFLDNQHQVLSFEPLFKGTVDQTSVYPRVVVQRAL
                     ALNASALILAHQHPSGTTEPSAADRAITERLKSALATVDVRVLDHFIVGKGSPYSFAE
                     AGLL"
     gene            4393..>4836
                     /locus_tag="VIO83_02485"
     CDS             4393..>4836
                     /locus_tag="VIO83_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HEY8330182.1"
                     /translation="MPAHSLNLPAASRRPLATRLAAGLCAALLGSIAAAADVLVVTDS
                     RHPVQAPAGVRIIELDQATRIEVELAAHLPADPQQAAAAVRQRLHDGGEALQRRIGHA
                     YQGVADAWGLGIAKIPAVVVDRRYVVYGEPDVSRAVARINAYRSTQ"
BASE COUNT          810 a         1682 c         1580 g          764 t
ORIGIN      
        1 caggccggcg cccccgaacc cggcgcggcg gtccaaggcc gcaggccggc gaccgtggcc
       61 gacgagcagg cgctgtacga cgtcgcgccg tcgttcgcgg aactgctgcc ttgggtggag
      121 ttcctgccgc agtcgaagtc catgctgctg gaggacgggc agtcggtggc ggccttctac
      181 gagctggtgc cactgggcac cgagggccgg gaacccggct ggctcgccca tgcccgcgat
      241 gccttggaga acgcgcttca ggactcgttc gatgaactgg acgagaaccc ctgggtactc
      301 cagctctacg cccaggacga acccagcttc gaccagtaca tgcagaccct gcgcgactac
      361 gtgcagccgc gcgcccgtga tacggccttc accgagttct atttgcgctt ctttagccac
      421 catttgcgcg cggtcgcgaa gccgggcggg ctgttcgagg acacggtggt cacgcggctg
      481 cgctggcgcg ggcagacgcg gcgcgtgcgc atggtcatct atcgccgtgc gaccggacag
      541 gccagccgcc gcggccagac gcccgagcag atgctgaaca tcgtctgcga ccgcctgtgc
      601 ggcgggctgg cgaacgccgg catccaggca cggcgcatgg tcgcagccga cgtccacgac
      661 tggctgctgc gctggttcaa tccgcgcccc gcaatgctcg ggccgagtgc agaggaccgg
      721 gagcgcttct acgcactggc gcgctatccc gacagcaccg aggaaagcga ggacggcgag
      781 atcgagctgg cgagcgggcg ggatttcagc cagcggttgt tcttcggcca gccacgttcc
      841 gacgtggagc acggcacctg gtacttcgac ggtatgccgc accgcgtgct gatcaccgac
      901 cggctgcgca tgccgcccgg caccggacac ctgaccggcg agacccgcaa aggggatgcc
      961 atcaacacgc tgttcgacca gatgccggaa gacaccctga tgtgtctcac gatggtcgcg
     1021 acgccgcagg atgtgctgga agcggatctc aaccacctgg cgaagaaggc cgtgggcgag
     1081 acgctggcgt cggagcagac gctcaaggac gtgcaggaag cccgctccct gatcggcagc
     1141 gcacacaagc tctaccgggg aacgctggcg ttctacctgc gcgggcgcga tgaggcagaa
     1201 ctggaccggc gcggcctgga cttggcgaac gtgatgctca acgccggctt gcagccggtg
     1261 cgcgaggacg acgaggtggc accgctcaac agctacctgc gctggctgcc gtgctgctac
     1321 aaccccggcc aggatcggcg caagtggtac acccaactga tgttcgccca gcacgcggcg
     1381 aacctgtcgc cggtgtgggg ccgcgcccag ggtacggggc accccggcat cacgatgttc
     1441 aatcgcggtg gcgggccaat caccttcgat ccgctcaacc gcctggaccg acagatgaac
     1501 gcccatctgt tcttgttcgg ccccacgggt tctggcaaga gcgccacgct caacaacttg
     1561 ctgaaccagg tcacggccat ctaccgcccg cggctgttca tcgtggaggc cggcaacagc
     1621 ttcggcctgt tcagcgattt cgcacgcagg ctcgggctaa cagtgaaccg ggtcaagctg
     1681 gcccctggct cgggcatcag cctcgcacca ttcgccgacg cacgccggct gatcgaaacg
     1741 ccgagcgacg tgcagacgct cgatgccgac gcgctggatg aagacctgcc accggatgcc
     1801 tcggccatgg aggcggacga gcagcgcgac gtgctgggcg aactggaaat cacggcgcgg
     1861 ctgatgatca cgggcggcga ggacaaggaa gaagcccgga tgactcgggc cgaccgctca
     1921 ttgatccgcc agtgcatcct cgacgccgcc gagcactgcc acggtggagg tggtagcgag
     1981 aagcgcaccg ttctcacgcg cgacgtgcgc aacgcgctgc gcacccgcgg ccaggacccg
     2041 acgttgcccg agatgcggcg cgtgcggctg ctggagatgg cggacgcaat ggacatgttc
     2101 tgccaaggca cggacggcga gatgttcgac cgcgatggca caccgtggcc cgaggccgac
     2161 atcactctgg tcgatctggc gacatacgcc cgcgagggct acaacgccca gctctccatc
     2221 gcctacatca gcctgatcag cacggtgaac aacatcgccg agcgcgacca gtacctggga
     2281 cgcccgatca tcaatgtgac cgacgaaggc cacgtgatca ccaagaaccc gctgctcgcg
     2341 ccctacgtgg tcaagatcac gaagatgtgg cgcaagctcg gtgcatggtt ctggctggcc
     2401 acgcagaaca tcgacgatct gccacgcgcc gcagagccga tgctcaacat gatcgagtgg
     2461 tggatctgcc tgtcgatgcc acccgatgaa gtcgagaaga tcgcgcgctt ccgcgaactc
     2521 aacgcttcgc agaaggcgct gatgctctcg gcacgcaagg aggccggcaa gttcagcgag
     2581 ggcgtcatcc tgtccaagtc gatggaagtg ctgttccgcg ctgtgccgcc cagcctctac
     2641 ctggcgatgg cgatgaccga gcccgaggag aaggccgaac gcttccagtt gatgcagcgg
     2701 cacggcatca gcgagctgga tgccgcgttg cgcgtggccg agaagatcga ccgtgcgcgg
     2761 ggcatcgaac cgctggcgct ggacacgctc gcctgacggg cgcgacacga tgcgcatgcc
     2821 ctcatccgcc cgccgtcatc cgaggatcgg cctgctgatc gtggcgacga ttggggttgc
     2881 gttgatgctg ctgcgcgtgc cgcatccggc aaccgagtcc atgcccccgg ccgccgccgg
     2941 gtccaaagcg cccaaaccgg ccggcccgcc ctggctgtat ggccgtgccg atgcgcgctt
     3001 caccgtggtc ggctacgccg acctggagtg tccgtactgc cgggcccact tcccggcgct
     3061 caagcgctgg atcgacgccc atcccgaggt gaactggcag tggcgccacc tgccgctgtc
     3121 catgcacgag ccggccgcga ccgtcgaggc acgcctggcc gagtgcgcgg gcgaagccgg
     3181 cggacatgcc gcgttctggc aggccgtggc atggctctac gcgaacaccc gtggcgacgg
     3241 ccagggcctg ccggagggcc tgcgctatcc cgccctcacg ccagccatgc aggcttgtct
     3301 cgacagcgag cgccccgatg ccgtcatccg tgcccaggcg gtggaagcgg cacagcaggg
     3361 catcgcggcc acgccgaccc tgctactgcg cgatcgcgac actggcaaga ccctcctgct
     3421 acacggcccc atggacggcg acgccttgct ctcggcgctc gacctgctgg ctgccggcga
     3481 cacggcgagc gccgagtcct cacatccccc agacatgccc gccggcgttg ccggtgacat
     3541 gcccaggtag ccatcgatct tcgaggctac ggcgcggctc gtccgcgttg atcgaaaccc
     3601 gttcgcatcg catcccttga cgcgaacagc caccgcatcc gcggtggtgg atgcccgctc
     3661 gtcacctgtt ccatccccat cgtccccagg agggtttgcc ctccaggggc aggcgccctc
     3721 cgatctcatc cattggaggt tcgccatgtc tctcgtcatc gccgactccc gcccggagtc
     3781 gctcaccctg atcgccgccc agcacgaaga ctggatcatc cggcaggcca tcaccctgct
     3841 ggagaaccgc gtgttcaagg ccggtccagc gctgggcagt cccgccgccg tgcgcgacta
     3901 cctgcgcctg aaactggtcg cggagccgaa cgaaatcttc gccgtcgtgt ttctcgacaa
     3961 ccagcaccag gtgctctcct tcgagccgct gttcaagggc acggtcgacc agacttccgt
     4021 gtatccgagg gtggtggtcc agcgtgcgct ggcgctcaac gcttcggcgc tgatcctggc
     4081 gcatcagcac ccctcgggga ccaccgagcc atcggccgct gatcgtgcga tcaccgagcg
     4141 gctgaagagt gccctggcca ccgtcgatgt ccgggtgctg gatcacttca tcgtcggcaa
     4201 gggcagcccg tactcgttcg ccgaagccgg cttgttgtag cgcactcatt tcatcaccac
     4261 gggagccctt ggctcccgtt tcttttcgcc ggtgcgcagg aaatcgggac tgaccggttt
     4321 cggtttcttt gtcgtcccct gatctgggtt gcgctcgact acgagtctgc atcgactccg
     4381 cggagacgcg acatgccggc tcattccctc aaccttcctg ccgcttcgcg gcgccccctg
     4441 gccacgaggc tggccgctgg actgtgcgcc gcgctgctcg gttccatcgc ggcagccgcc
     4501 gatgtgctcg tcgtcaccga cagccgtcat ccggtgcagg ccccggccgg cgtgcggatc
     4561 atcgagctgg accaggccac gcgcatcgag gtcgaactcg cggcgcacct gccggccgac
     4621 ccgcaacagg ccgcagcggc ggtgcggcag cggttgcatg acggcggcga ggcgctgcag
     4681 cgccgcatcg gccatgccta ccaaggtgtc gcggatgcct ggggactggg catcgccaag
     4741 attcccgccg tggtggtcga tcgacgctac gtggtctatg gcgagcccga cgtgtcccgc
     4801 gccgtcgcgc gcatcaacgc ctaccggagc acgcag
//
LOCUS       DATCUN010000065        38229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_58653, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000065 DATCUN010000000
VERSION     DATCUN010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 38229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38229
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58653"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VIO83_02490"
     CDS             complement(<1..316)
                     /locus_tag="VIO83_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8330183.1"
                     /translation="MLELTPRALRLMDGYDDGEEARHFRLRIERPQAEDRAVIPGQFF
                     MLGLPGLGEAPFTYVSPPNGPGEFSALIRRSGRLTAQLFERTPGAVLGYRGPFGKGWP
                     LLL"
     gene            complement(304..1338)
                     /locus_tag="VIO83_02495"
     CDS             complement(304..1338)
                     /locus_tag="VIO83_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY8330184.1"
                     /translation="MTAVEPACYALDSVERLRAHLAGGRTLYEVREDGQGGRHWQSLA
                     ADDAQPFVPQREPPPFSAKAFFFAEREAIFRFEGDRFVACTPEVPAQVLFGLHACDLA
                     AIAYQDQFFAADAHYQQRRAATLLVGLDCRSSCSQGFCTLVDSGPDVRAGTADLVLLP
                     TDAGWLLLSASAAGAAAVADLGLPAASLDWQAQRAANRQRVASEQGDGQWLQDGIAAI
                     NAGQVDAATWDSLGLRCLVCSGCTTVCPTCSCFAPLERQLPGGAVQHERVWDSCLYEG
                     FQKEASGHNPAAMPGQRVQRFWQHKFGEGFRREFGRYGCIGCGRCDQVCPGGIGVHAV
                     MHKVGKPCSN"
     gene            complement(1325..3439)
                     /gene="fdhF"
                     /locus_tag="VIO83_02500"
     CDS             complement(1325..3439)
                     /gene="fdhF"
                     /locus_tag="VIO83_02500"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEY8330185.1"
                     /translation="MAGGPAMQVVPSICTFCGVGCGMGLRVERGRVIGVEPQPGHPVS
                     QGQLCSKGWSTSFAIDTADRLTRPLIREAGGFRPASWDEALARVAAAFGEALVEGGPT
                     AVGVISCARATNEDNYAAQKFARAVLGTHNIDHCARICHSPTVAGLSLTLGSAAMTGS
                     IADVDAADLILLIGTDTTENHAMIGARILRAQARGARLVVVDPRQTRLARLADQHIPL
                     KLGSNIALLNGLLQIIFANGWENRAFLEARCENVEALRQHVASLTPELSSAQTGVPVA
                     ELEKLAYAVSHAKAAFIAYGMGITQFQSGTNNVVAVSNLALVCGQIGRPGAGIFPLRG
                     QNNVQGACDMGCLPKVYPGYQLVDSAEVQDGFARAWGVSLPQAPGLTSMGMTKAALAG
                     DFKALMIMGEEPVVTDPDQHQVEHALRALDFLVVVELTMTETAKLADVVLPAASFAEK
                     DGTFTNCERRVQRIRRAIAPPGEAWTDWQILGALAARMGYLGMIWADAEAIFDEMAAL
                     APLFSAMSYPALDTHHGLHWPCDTAHPQGSPLLHAQAFPRGRGRLLPVTQVDPDECPD
                     ADYPFFFTTQRLHFHYGGGSMTRKSPLLERETPAGLLFIHPQDAADLRLEDGQGVCLR
                     SRRGTLETRAQITDDVPPGTLSMPYHFREAPCNILTNSAQDPQSKMPELKACAVAIEP
                     LPPGVAPRLANALDQEVADDRR"
     gene            3571..4338
                     /gene="fnr"
                     /locus_tag="VIO83_02505"
     CDS             3571..4338
                     /gene="fnr"
                     /locus_tag="VIO83_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEY8330186.1"
                     /translation="MLENSGLQPSGRCQRERISCRDCAIRPHCFPANLHGSELQQLDD
                     IIQRSRPLHKGEHLFAAGESLQQLHAVRTGTLKTYLLSSEGEEQITGFHLPGDVLGLD
                     GLGAFSHPSFAVALETSMVCSIPLTRLEELAGSIPRLRAELLRVMSAAIHAEHEQLRL
                     TRKGAEERLGTFLLELSQRFRQRGYSAQNFILPMSRCEIANYLGLTSETVSRLLARYR
                     DRGLVAISGREVRLTDIRRLQQLCGLGAAELADGPAA"
     gene            4439..4993
                     /locus_tag="VIO83_02510"
     CDS             4439..4993
                     /locus_tag="VIO83_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY8330187.1"
                     /translation="MAQPENKTENQIPVQTEDKAAPQNPLATALRPLENLRRQVDRLF
                     EDFDRGWHLPFARNTAFDREPFWRRELSFSGMPAVDIVEKSDAFEISAELPGMDEKNI
                     EIKLSNGTLTIKGEKKDEREEKKKDYYLSERHYGSFQRTFGLPEGVDPEKIEAHFSKG
                     VLTLHLPKKPDAQKPEKTIPIKTS"
     gene            5122..7677
                     /gene="mgtA"
                     /locus_tag="VIO83_02515"
                     /pseudo
     CDS             5122..7677
                     /gene="mgtA"
                     /locus_tag="VIO83_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018928918.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
     gene            7773..10193
                     /locus_tag="VIO83_02520"
     CDS             7773..10193
                     /locus_tag="VIO83_02520"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8330188.1"
                     /translation="MPAPHELTAQQLYHACDVAELGCDSTSDLPDLEASLGQERALAA
                     LNFGVGIRHEGYNLYVMGSPGLGKHAIVRHLLGQQAAQIEPPSDWCYINNFRTPHKPR
                     VLRLPAGTGSGLQADMRALVRSLLTALPAAFDSDDYRNAMQALKDKFKAREEELFGSV
                     DSQARASGIILLRTPAGFTLAPLKDGEVLDAEAFDKLSAEEKQQIEEHTERIRQVLRQ
                     ALQQGAAASREHDQQVEQLNEAVTRATVERQFIDLEAHYSRLAEVAAFLAEVKLDIVE
                     KGLEIFGSAPAEKLLDRQRLTPFNRYFVNVLVDNGGSKGAPLVYEDYPSYQNLIGRIE
                     HQAHMGTLHTDFTLIKGGALHRANGGYLLLDVRKLLTTPFAWEALKRALQARELRIQS
                     LEQILSLASTISLEPDPIPLEVKVVLIGDRLLYYLLTQYDPEFPLLFKVEADFAEDLP
                     RSADNTRLYAQLIATLQRREQLRPLDGPAVARLIEQAAREAADGEKLSLHLQDLLDLM
                     READYWAGQQQRTQVRREDVEQAVRERLHRADQLRERLLEAALRDLRHIDTDGQCVAQ
                     INGLAVLQLGAHAFGHPARISATARLGDGKLIDIEREVELGGALHSKGVLILSAYLAS
                     RFGGSQPLSFSASLVFEQSYGGIDGDSASAAELCVLQSALADLPLRQDLAITGSVDQH
                     GRMQVIGAVNEKIEGFFDLCAARGLSGSQGVIIPHGNIKHLMLRREVVAAVAAGHFHV
                     HAVEHAEQAMALLTGLPAGEVDAQGQFPAGTINGAVQRRLHELSELRQRYAHPQSDGP
                     AGNGQADM"
     gene            10255..11382
                     /locus_tag="VIO83_02525"
     CDS             10255..11382
                     /locus_tag="VIO83_02525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008018111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEY8330189.1"
                     /translation="MASVENPLSLFLLGDVMPARGIDQILPHPGDPRLYEDYVHDARD
                     YVVLAEQRCGPIERPVDFAYVWGDALGELQRRQPQIRLINLESAVTRHGRPEPKGINY
                     RMTPENFPLLRAAGINCCTLANNHVLDWGEVGLRDTLDTLTAHGMPWAGAGLDRASAE
                     APAVLALPDGRRLLVFAFGLPDSGIPAHWAAGPHQAGVARLDDLSPRSLAHVAGLIHA
                     SRQTGDRVLVSLHWGDNWDFAVPAEHRAFAHGLIDQAGVDLVNGHSSHHIKGIEVHRE
                     RLILYGCGDRLNDYEGIEGHADFRGELGLLYFVRLHDDGRLLGLDMVPTRLQRLRINL
                     AEGVDRRWLFDTLARECATFGGGIRQQIDGSFALTWPRGAP"
     gene            11379..13097
                     /gene="polX"
                     /locus_tag="VIO83_02530"
     CDS             11379..13097
                     /gene="polX"
                     /locus_tag="VIO83_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEY8330190.1"
                     /translation="MSVSNAEIAAIFEEIADILELEAANPFRIRAYRNAARTLQAFAP
                     DIAGLLASGQSLPKLPGIGEDLAAKIGEIAATGSCAILPRLRSEVPAGLRELLKVSTL
                     GPRRVARLWHELGIQTPAELQQAARAGRIRQLHGFGEQTEARLLQAVELLLHTPQRWL
                     LSRVAPIAERLVAELGKVPGVIEVASAGSFRRGRDTVGDLDILVNARRGSAVIQRFTS
                     DAEVGRVLAAGTTRASVLLNSGLQVDLRLLSPPAWGAGLLYLTGSKAHNIALRRMAQE
                     RGLKISEYGVYRGKQRIAGSSEDSMYQALGLPWIAPELREDRGEIEAALAGTLPALVE
                     LGDLRGDLHAHTRASDGHDTLEEMAAAAQAAGLEYLAITDHSSRMAITHGLDADGLAR
                     QIEQIDALNERLSGITLLKGIEVDILEDGSLDLPDAILGRLDLVVGAVHSAFNLSKEQ
                     QTRRLLRALEHQHFSILAHPSGRLLGERPPINCDFAALLAAARQRGCALELNAQAERL
                     DLDDLHCREARDAGVPIAISSDAHRTLDFAGLRFGIGQARRGWLAAKDVLNTRPLAEL
                     RRWLKH"
     gene            complement(13196..15100)
                     /gene="htpG"
                     /locus_tag="VIO83_02535"
     CDS             complement(13196..15100)
                     /gene="htpG"
                     /locus_tag="VIO83_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEY8330191.1"
                     /translation="MSVETRKETLGFQTEVKQLLQLMIHSLYSNKEIFLRELISNASD
                     AADKLRFEALAKPELLEGGDPLKIRVSFDKDARTVTLDDNGIGMSRDDVISHLGTIAK
                     SGTADFLKNLSGDQKKDSQLIGQFGVGFYSAFIVADQVDVYSRRAGLPASEGVHWSSK
                     GEGEFDIAAIDKAERGTRIVLHLKSDETEFADGWRLRNIIKKYSDHIALPIELPKEHF
                     GEEKDKPAEVEWETVNRASALWTRPRSEVKDEEYQEFYKHVAHDFENPLSWSHNKVEG
                     KLEYTSLLYVPGKAPFDLYHREAPKGLKLYVQRVFIMDQADQFLPLYLRFIKGVVDSN
                     DLSLNVSREILQSGPVIDSMKSALTKRVLDMLEKLAKDKPDDYQTFWKAFGQVLKEGP
                     AEDFANKEKIAGLLRFASTSDASGEQSVALADYLGRLKDGQDKVYYLTGESYAQVKNS
                     PHLEVFRKKGIEVLLLTDRIDEWLMSYLTEFDGKQLIDVARGDLDLGKLDSEEDKKAQ
                     EEVAKAKEGLVERLKKALGEQVAEVRVSHRLTDSPAILAIGEQDLGLQMRQILEASGQ
                     KVPESKPIFEINPAHPLIEKLDAEADESRFADLSHILFDQAALAAGDSLKDPAAYVQR
                     LNKLLVELSA"
     gene            complement(15202..16449)
                     /locus_tag="VIO83_02540"
     CDS             complement(15202..16449)
                     /locus_tag="VIO83_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8330192.1"
                     /translation="MDALLIVGGLLLILFGMVWLVVQAFSTSLLWGVGSLLPPLPLLF
                     IVRHWRRARSSVTLMGMGCIPLVVGLTLLASHDADRLAAILSLRWLQPEAKVARELDI
                     QLRGEFNGKPFAPELGELIDGTLILREGDDFFARRELSIRLPSQPSGDLQLNVLPEDR
                     DNLPAIELSWLLPDHDQPDTRRITSGYTLHLDLKSAEPNLLRGDFHLVLPSGYRTSLS
                     GKVELYTSHLRYRNGRVDTRHNSQATLEWLIVDYLQRNARTRAVELAPLPPLDLDSHQ
                     LDLDVQARIAGVAKRMPLTLARSDLHGWRVQGDQAPALPPPSEDDLLRTAPVPTTVVR
                     GEERPLDRRIGFSLESLLDRPGRYLGARVRVMTERGRSAEGRFDGINPEGRLVIQHAL
                     GGQGEASFLLRSSEVTQIELLEP"
     gene            16787..17092
                     /locus_tag="VIO83_02545"
     CDS             16787..17092
                     /locus_tag="VIO83_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330193.1"
                     /translation="MQKPLTYALLLAATLGLAACGDQADKKAESAAGSAPQPTQSMGE
                     AAKKATDAAKEAGAVVEQKAAEVTEQAAGAVKDAGAAVEQKAAEVQQEAGKVEEEKK"
     gene            complement(17228..17959)
                     /locus_tag="VIO83_02550"
     CDS             complement(17228..17959)
                     /locus_tag="VIO83_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 family protein"
                     /protein_id="HEY8330194.1"
                     /translation="MPFEQHLPHFIAVSWFVLCWWGYTRYAIWKGGSTPCLASVLHLY
                     REDWMRRLLMRDNRIADANVIGNLERNASFFASSTLIILAGILTVLGSTERAVSVLAE
                     LPFVQAVSREIAEVKIIAVAVVFIYSFFTFTWCMRQYNFAAMMVGAAPMLTEERVTEQ
                     ERKSYAERTARVISMAANQFNFGLRSYYFGLATLAWFINPWFYMLATAGVVFVLYRRE
                     FHSDVLAVMVYSPVELGQSAKQQGG"
     gene            complement(18082..19389)
                     /gene="brnQ"
                     /locus_tag="VIO83_02555"
     CDS             complement(18082..19389)
                     /gene="brnQ"
                     /locus_tag="VIO83_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEY8330195.1"
                     /translation="MNHLKTADVLGLGFMTFALFLGAGNIIFPPSVGMAAGASVWSAA
                     LGFLLTAVGLPLLTVVALARVGGGMARLTAPLGRRAGLLFAVAVYLAIGPLFATPRTA
                     VVSFEMGVASFTGNAPLPLGLYTLAYFAAVLFLVLNPGRLLERIGKMITPVLIIGLLL
                     LGGAAVFAPAGEVGNSIAPAYREAPLLQGFLQGYLTMDTLGALVFGIVIATAIRDHGI
                     DMAHRVTRYSILAALIAATGLALVYLALFRLGATSQGIAAGAQNGVQILTTYVRYTFG
                     MPGELLLALVITLACLTTAVGLIAACGEFFRSQFGLPYRTVVVLTSLFSLLLANQGLT
                     QLIGVSLPVLVGLYPLAIVLVLLSLADRLWRHPPRVFVPVMAVALVFGVSDGLVAAGF
                     KAAVPAFLARLPLAEHSLGWLLPVLATLLLVAVIDRALGRPQA"
     gene            complement(19488..20369)
                     /gene="sucD"
                     /locus_tag="VIO83_02560"
     CDS             complement(19488..20369)
                     /gene="sucD"
                     /locus_tag="VIO83_02560"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017132983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY8330196.1"
                     /translation="MSILIDKDTKVICQGFTGSQGTFHSEQALEYGTRMVGGVTPGKG
                     GTTHLGLPVFDTVKDAVAATGATASVIYVPAPFCKDSILEAAFGGIQLIICITEGIPT
                     LDMLEVKVKCDELGVRLIGPNCPGVITPGQCKIGIMPGHIHLPGKVGIVSRSGTLTYE
                     AVKQTTDAGFGQSTCVGIGGDPIPGSSFIDILKLFEQDPQTEAIVMIGEIGGSAEEEA
                     AAFIKANVSKPVVSYIAGVTAPSGKRMGHAGAIISGGKGTADEKFAALQDAGVKTVRS
                     LADIGKALAEATGWTMK"
     gene            complement(20366..21535)
                     /gene="sucC"
                     /locus_tag="VIO83_02565"
     CDS             complement(20366..21535)
                     /gene="sucC"
                     /locus_tag="VIO83_02565"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY8330197.1"
                     /translation="MNLHEYQGKQLFAEYGLPVSKGYAVDTPKEAAEACDKIGGTAWV
                     VKAQVHAGGRGKAGGVKLVRNKEEAKAFAQQWLDQRLVTYQTDANGQPVSKILVEACT
                     DIAKELYLGAVVDRSSRRIVFMASTEGGVDIEKVAHETPEKILKVSIDPLVGAQPFQG
                     RELAFQLGLQGDQVRQFAQIFVGLAQLFEDYDLALLEVNPLVIKADGNLHCLDAKLNI
                     DGNALYRQPRLREMQDPSQDDPREAHAAKWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     NLHGGKPANFLDVGGGATKERVVEAFKIILSDSNVKAVLVNIFGGIVRCDMIAEGIIG
                     AVREVGVQVPVVVRLEGNNAELGAGVLAESGLNIMAATSLTDAAQKVVKAAEGKQ"
     gene            complement(21748..23184)
                     /gene="lpdA"
                     /locus_tag="VIO83_02570"
     CDS             complement(21748..23184)
                     /gene="lpdA"
                     /locus_tag="VIO83_02570"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY8330198.1"
                     /translation="MTQKYDVVVIGGGPGGYVAAIRAAQLGLKTACIEKYKGKEGKVV
                     LGGTCLNVGCIPSKALLDSSWKYHEAKESFGVHGIEVKGVSMDVPAMVERKASIVKNL
                     NTGVAGLFKANGVTLVEGHGKLLAGKQVEVTAEDGSVQVLEATHVIIASGSKPIDIPP
                     APADHDIIVDSTGALEFQSVPKKLGVIGAGVIGLELGSVWARLGAEVTVLEAQDKFLA
                     AVDEQIAKEALKLLTKQGLDIRLNARVTGSEVKKKQVTVHFTDAAGEQQLTFDKLIVA
                     VGRRPVTQDVLAADSGVTLDERGFIFVDDQCATSVPGVYAIGDVVRGPMLAHKASEEG
                     VMVAERIAGHKAQVNYDLIPSVIYTHPEIAAVGKTEQQLKAEGVAINVGTFPFAASGR
                     AMAANDTHGLVKVIADANTDRVLGVHVIGPAAAELVQQGAIGMEFGTSAEDLGMMVFS
                     HPTLSEALHEAALAVNGHAIHVANRKKR"
     gene            complement(23254..24459)
                     /gene="odhB"
                     /locus_tag="VIO83_02575"
     CDS             complement(23254..24459)
                     /gene="odhB"
                     /locus_tag="VIO83_02575"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEY8330199.1"
                     /translation="MAIEIKAPTFPESVADGTVATWHKKPGDAVKRDELIVDIETDKV
                     VMEVLAEVDGVLAEIVANEGDTVLSGQLLGKLNEGAVAAAPAAAAAAPAAAAPAAAEE
                     AILSPAARKIAAENDLDRNAIPGTGKGGRVTKEDAVAAVDAKKAAPAPAAKPAAPAAD
                     APIFAAGDRVEKRVPMTRLRAKIAERLVEAQSSMAMLTTYNEVNMKPIMDLRAKYKDL
                     FEKKHNGVRLGFMSFFVKAATEALKRFPGVNASIDGGDIVYHGYSDVGVAVSSDRGLV
                     VPVLRNAELMSLAEIEGTIGAYGRKAKEGKLTIEEMTGGTFTISNGGVFGSLLSSPIV
                     NPPQTAILGMHKIQDRPMAVNGQVVILPMMYLALSYDHRLIDGKEAVSFLVAIKDLLE
                     DPARLLLDV"
     gene            complement(24541..27372)
                     /locus_tag="VIO83_02580"
     CDS             complement(24541..27372)
                     /locus_tag="VIO83_02580"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY8330200.1"
                     /translation="MHESVMQRMWNSAHLSGGNAAYVEELYELFLHDPNAVPEEWRTY
                     FQKLPTGGNQSPDVSHATVRDHFVLLAKNQRRAQPVSAGAVSSEHEKKQVEVLRLIQA
                     FRMRGHQASRLDPLGLWQRQAPADLSIHHYGLTDADLDTVFRTGDLYIGRQEATLREI
                     RDGLQATYCGTIGAEFTHIVDAEQRNWFAQRLESVRGRPEFSVEAKSHLLERLTAAEG
                     LEKYLGTKFPGTKRFGLEGGESMVPMVDEIIQRSGSYGAKEIVIGMAHRGRLNLLVNA
                     LGKNPRDLFDEFEGKRVEGLSSGDVKYHQGFSSNVMTPGGEVHLAMSFNPSHLEIVSP
                     VVEGSVRARQDRRGDAVGDKVVPISIHGDAAFAGQGVVMETFQMSQIRGFKTGGTIHI
                     VVNNQVGFTISNTEDSRSTEYCTDPAKMIQAPILHVNGDDPEAVLFVTQMAVDYRMQF
                     KRDVVIDLVCYRRRGHNEADEPSGTQPLMYQKIAKQRTTRELYADALVQAGALSTAQV
                     EAKVEEYRTALDNGQHVVKSLVKEPNAELFVNWTPYLGHAWTAQHDTSFDLKTLQELN
                     AKLLQIPEGFVVQRQVAKIYEDRGRMGVGAMPINWGCAETLAYATLLKEGHPVRITGQ
                     DVGRGTFSHRHAALHSQKDASRHIPLQHLFDGQPKFELYDSFLSEEAVLAFEYGYATT
                     QPNALVIWEAQFGDFANGAQVVIDQFITSGEHKWGRLCGLTMLLPHGYEGQGPEHSSA
                     RLERYLQLCAEQNIQVCVPTTPAQVYHMLRRQVIRPLRKPLVALTPKSLLRHKLAIST
                     LEDLAQGSFQPVLPEIDSLDPKKVERLVLCSGKVYYDLLEKRRAEGREDTAIVRIEQL
                     YPFPEEELAEVMAAYKNLKHVVWCQEEPMNQGAWYCSQHHMRRVITAYKKTLGLAYAG
                     RDASASPACGYASMHAEQQEKLLQDAFTI"
     gene            complement(27623..28327)
                     /locus_tag="VIO83_02585"
     CDS             complement(27623..28327)
                     /locus_tag="VIO83_02585"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY8330201.1"
                     /translation="MLQVSVYRYNPEKDAAPFMQDFQVNTDGKDLMVLDVLALIKEQD
                     VTFSYRRSCREGVCGSDGMNISGKNGLACITPLSAVVKNGKLVIRPLPGLPVIRDLVV
                     DMSIFYKQYEKVQPFLQNDTPAPAIERLQSPEQREKLDGLYECILCACCSTSCPSFWW
                     NPDKFLGPAALLQAYRFLADSRDTKTEERLASLDDPFSVFRCRGIMNCVNVCPKGLNP
                     TKAIGHVRNMLLQSGT"
     gene            complement(28339..30111)
                     /gene="sdhA"
                     /locus_tag="VIO83_02590"
     CDS             complement(28339..30111)
                     /gene="sdhA"
                     /locus_tag="VIO83_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEY8330202.1"
                     /translation="MANIPTLSFDAIIIGGGGAGMRAALQLSQSGHKTAVVTKVFPTR
                     SHTVSAQGGITCAIASADPNDDWRWHMYDTVKGSDYIGDQDAIEYMCSVGPEAVFELE
                     HMGLPFSRTEQGRIYQRPFGGQSKGPDNPTQAARTCAAADRTGHALLHTLYQANLKNG
                     TTFLNEWFAVDLVKNQDGAIVGIIAICIETGETAYIRAKATVLATGGAGRIYASTTNA
                     LINTGDGIGMALRAGVPVQDIEMWQFHPTGIAGAGVLVTEGCRGEGGYLINKHGERFM
                     ERYAPNAKDLAGRDVVARSMVKEIIAGNGCGPDGDHVLLKLDHLGEEVLHSRLPGICE
                     LSKTFAHVDPVTAPVPVVPTCHYMMGGVATNIHGQAITQDANGNDKIIDGLFAVGEVA
                     CVSVHGANRLGGNSLLDLVVFGRAAGLHLEKALKEGIEHRGATESDLEASLKRLSGVN
                     ERTTGEDVAPLRKELQNCMQNYFGVFRTGEYMQKGIAQLAELRERIANVKIADKSQAF
                     NTARTEALELQNLLEVAEATAIAAEARKESRGAHAREDFEDRDDENWLCHSLYFPGEK
                     RVTKRGVNFSPKTVPAFEPKVRTY"
     gene            complement(30115..30483)
                     /gene="sdhD"
                     /locus_tag="VIO83_02595"
     CDS             complement(30115..30483)
                     /gene="sdhD"
                     /locus_tag="VIO83_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEY8330203.1"
                     /translation="MVTNVTNFSRSGLYDWMAQRVSAVVLAAYFLFLLGYLVASPSLT
                     FSDWHGLFSQNWMRIFSLLGIVALSVHAWVGMWTISTDYLTPMALGKAATAVRFLFQA
                     VCGIAMFAFFVWGVQILWGI"
     gene            complement(30477..30851)
                     /gene="sdhC"
                     /locus_tag="VIO83_02600"
     CDS             complement(30477..30851)
                     /gene="sdhC"
                     /locus_tag="VIO83_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007244768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEY8330204.1"
                     /translation="MNSKRPVNLDLRTIQLPVTAYTSIAHRISGVILFLGIAVLLYAL
                     DLSLASEEGFEQVKACLTSPLAKLVIWGLLSALLYHLVAGIRHLIMDMGIGETLEGGK
                     LGSKIVIAVAAVLIVLAGVWVW"
     gene            31224..32510
                     /gene="gltA"
                     /locus_tag="VIO83_02605"
     CDS             31224..32510
                     /gene="gltA"
                     /locus_tag="VIO83_02605"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY8330205.1"
                     /translation="MADKKAQLIIEGAAPVELPILSGTMGPDVIDVRSLTNQGVFTYD
                     PGFMSTAACESKITFIDGDKGVLLHRGYPIEQLAEKSDYLETCYLLLNGELPNAEQKA
                     HFTGLVKNHTMVHEQMKSFLNGFRRDAHPMAVMCGMVGALSAFYHDSLDNNNPQHREI
                     SAIRLIAKMPTIAAMAYKYSAGQPIMYPRNDMGYAENFLHMMFSTPCEDKPVNPVLAR
                     AMDRIFVLHADHEQNASTSTVRLAGSTGANPFACIAAGIAALWGPAHGGANEAVLTML
                     DEIGSVENIEKFIAKAKDKNDPFKLMGFGHRVYKNFDPRAKVMKKTCDEVLQELNIND
                     PKLELAMKLEEIARNDPYFKERNLYPNVDFYSGIILKAIGIPTSMFTVIFALARTAGW
                     ISQWKEMLSTPYKIGRPRQIYTGYPTRDYAPLNSRK"
     gene            32699..32893
                     /locus_tag="VIO83_02610"
                     /pseudo
     CDS             32699..32893
                     /locus_tag="VIO83_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
     gene            complement(32978..33229)
                     /locus_tag="VIO83_02615"
     CDS             complement(32978..33229)
                     /locus_tag="VIO83_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020799973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY8330206.1"
                     /translation="MQCRAGCGACCVAPSISSPIPGMPQGKPAGVRCVQLDEHNLCRL
                     FGQPERPAVCSRFSADHEVCGETDAQAVRLLTELEQLTA"
     gene            33358..33702
                     /locus_tag="VIO83_02620"
     CDS             33358..33702
                     /locus_tag="VIO83_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEY8330207.1"
                     /translation="MPVWMQTAALLSLSNIFMTFAWYGHLKTLNGKPWIIAALISWGI
                     ALFEYLIMVPANRIGYSEMSVGQLKIMQEVITLSIFVPFSVLYMQQPLKLDYLWAGLC
                     LLGAVYFIFRSA"
     gene            complement(33722..34600)
                     /locus_tag="VIO83_02625"
     CDS             complement(33722..34600)
                     /locus_tag="VIO83_02625"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8330208.1"
                     /translation="MAKVAFLGLGVMGYPMAGHLARHGHELCVYNRTAETAQRWVAEH
                     GGRSAPTPREAVAGAEFVMSCVGNDDDLRSVVLGADGAFAGMAPGSVLVDHTTASASV
                     ARELAALAAERELEFLDAPVSGGQAGAQNGVLTVMVGGEPAVFARAEPVIQGYARMVR
                     LMGHAGSGQLTKMVNQICIGGLLQGLSEALHFAQCAGLDGNAAMEVISKGAAQSWQLE
                     NRHASMLEGRFDFGFAVDWMRKDLGILLDEARSNGAQLPVTALVDQFYADVQAAGGGR
                     WDTSSLITRLKPQVRS"
     gene            34700..35299
                     /locus_tag="VIO83_02630"
     CDS             34700..35299
                     /locus_tag="VIO83_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEY8330209.1"
                     /translation="MKHWLVIDLEATTDKNGWPVEEMEIIEIGASIVDEQGREQAHFQ
                     SFVQPTRHPQLTAFCRELTHIRQSDVDSAPTLRQLHATFEPWLQQHLGNLQGWLSWGD
                     YDRQQFEIEWRKQQIASCLASLPHFNLKKLFHRQFKGVVGRKRVGLNRALEMTGLTFL
                     GTQHRGLDDARNIARLLPATLPVLAAARLEAQQGQQARQ"
     gene            35496..>38229
                     /gene="metH"
                     /locus_tag="VIO83_02635"
     CDS             35496..>38229
                     /gene="metH"
                     /locus_tag="VIO83_02635"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY8330210.1"
                     /translation="MSDRAARLQALQNALKERILILDGGMGTMIQSYKLQEEDYRGER
                     FADWPSDVKGNNDLLLLSRPDVIQAIEKAYLDAGADILETNTFNATRVSQADYGMEAL
                     AYELNVEGARLAREVADAKTAETPDKPRFVAGVVGPTSRTCSISPDVNNPGYRNVTFD
                     ELVENYTESTRGLIAGGADLILIETIFDTLNAKAAIFAVQQVFEEEGVELPIMISGTI
                     TDASGRTLSGQTTEAFWNSVRHANPISVGLNCALGAKDLRPYLEELATKADTHVSAHP
                     NAGLPNAFGEYDETPAQMAAVVEEFAASGFLNIIGGCCGTTPPHIQAIAEAVAKYPPR
                     VIPEIPKACRLSGLEPFTIDRNSLFVNVGERTNITGSARFARLIREENYTEALEVALQ
                     QVEAGAQVIDINMDEGMLDSKAAMVTFLNLIAGEPDISRVPIMIDSSKWEVIEAGLKC
                     IQGKGIVNSISMKEGVEAFKHHARLCKRYGAAVVVMAFDEAGQADTAARKREICQRSY
                     DILVNEVGFPPEDIIFDPNIFAVATGIDEHNNYAVDFIEACAYIRDNLPYALSSGGVS
                     NVSFSFRGNNPVREAIHSVFLYHAIQNGLTMGIVNAGQLEIYDEIPKQLRDAVEDVVL
                     NRHAGSTEALLAIADQYKGDGSVKEAETEEWRSLPVGKRLEHALVKGITAFIVEDTEE
                     FRQQCARPIEVIEGPLMSGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAHLIPFIEAE
                     KGDKPEAKGKILMATVKGDVHDIGKNIVGVVLGCNGYDIVDLGVMVPAEKILQVAKEE
                     KCDIIGLSGLITPSLDEMVHVAKEMQRQGFTLPLMIGGATTSKAHTAVKIEPQYSNDA
                     VVYVTDASRAVGVATTLLSKELKPDYVAKLKTEYAEVRERTAARSSRTERLSYDK"
BASE COUNT         6898 a        12911 c        12097 g         6323 t
ORIGIN      
        1 cgagcagcag cggccagccc ttgccgaacg ggccgcgata gccgagcacg gcgccgggcg
       61 tccgctcgaa cagctgcgca gtcagccgac cgctgcgccg gatcagcgcg ctgaactcgc
      121 ccgggccatt cggcgggctg acataggtga acggcgcctc gcccagcccc ggcagaccga
      181 gcatgaagaa ctgcccggga atcaccgccc gatcctccgc ttgcggccgc tcgatgcgca
      241 ggcgaaagtg cctggcctcc tcgccgtcgt cgtagccatc catcaggcgc agggcgcgcg
      301 gcgtcagttc gagcatggtt tacctacctt gtgcatgacc gcatgcacgc cgatgccacc
      361 ggggcagacc tgatcgcagc gcccacagcc gatgcagccg tagcgaccga attcgcgacg
      421 aaagccctcg ccgaacttgt gctgccagaa acgctggacc cgctgccccg gcatggccgc
      481 gggattgtgc ccgctggctt ccttctggaa gccctcatac aggcaggagt cccatacccg
      541 ttcgtgttgc acggccccgc ccggcagctg gcgctccagg ggggcgaagc aggagcaggt
      601 cggacacacc gtggtgcagc ccgagcacac cagacagcgc agaccgagac tgtcccaggt
      661 cgcggcatcc acctggccgg cattgatcgc ggcgatgcca tcctgcagcc attgcccgtc
      721 gccctgctcg ctcgccacgc gctggcgatt ggccgcccgc tgcgcctgcc agtcaagact
      781 ggcagctggc aggccgagat cggcgaccgc cgcggcaccg gccgccgagg cgctcagcag
      841 cagccagccg gcatcggtcg gcagcagaac caggtcggcc gtgccggcgc gcacgtccgg
      901 gccgctgtcg accagcgtgc agaagccctg gctgcaactg ctacgacaat caagtcccac
      961 cagcagggtc gccgcgcggc gctgctggta atgggcatcg gcggcaaaga actggtcctg
     1021 ataggcgatg gcggccagat cgcaggcatg caggccgaac agcacctgcg ccggcacctc
     1081 gggcgtgcag gcgacgaagc gatcgccctc gaagcggaat atcgcttcgc gctcggcgaa
     1141 gaagaacgcc ttggccgaga acggcggcgg ctcacgctgg ggcacgaaag gctgcgcatc
     1201 gtccgctgcc aggctctgcc agtgacgccc gccctggccg tcttcgcgga cctcgtagag
     1261 cgtgcgcccg cccgccagat gggcacgcag gcgctcgacg ctgtccagcg cgtagcaggc
     1321 aggctcaacg gcggtcatca gcgacctcct ggtccagtgc attagccagc cgcggcgcca
     1381 cacccggcgg cagcggctcg atggccaccg cgcaggcctt cagctccggc atcttgctct
     1441 gcgggtcctg ggcgctgttg gtgagtatgt tgcagggcgc ctcgcggaag tgatacggca
     1501 tcgacagggt gccgggcggc acgtcgtcgg tgatctgcgc gcgggtttcc agcgtgccgc
     1561 gccgcgaacg caggcacacg ccctggccgt cctctaaacg caggtcggcg gcatcctgcg
     1621 ggtggatgaa cagcagcccc gccggggttt cccgctccag cagcggcgac ttgcgggtca
     1681 tcgagccgcc gccgtagtgg aagtgcaggc gctgggtggt gaagaagaac gggtagtcgg
     1741 catccgggca ctcgtcggga tcgacctggg tcaccggcag cagccgcccg cgcccgcgcg
     1801 gaaaggcctg tgcatgcagc agcggcgagc cctgcggatg ggccgtgtca cacggccagt
     1861 gcaggccgtg atgggtatcc agcgccgggt agctcatcgc ggagaagagc ggcgccagcg
     1921 ccgccatctc atcgaagatg gcctcggcgt cggcccagat catccccagg taacccatgc
     1981 gtgccgccag cgcgcccaag atctgccagt cggtccaggc ctcgcccggc ggcgcgatcg
     2041 cgcggcggat gcgctgcacg cggcgctcgc agttggtgaa ggtgccgtcc ttttccgcaa
     2101 aggacgccgc gggcagcacc acgtcggcca gcttcgccgt ctcggtcatg gtcagctcga
     2161 ccaccaccag gaagtccagg gcgcgcaggg cgtgctcgac ctggtgctgg tccgggtcgg
     2221 tgaccaccgg ctcctcgccc atgatcatca gcgccttgaa gtcgccggcc agcgccgcct
     2281 tggtcatgcc catcgaggtc aagcccggcg cctgcggcag cgacacaccc caggccctgg
     2341 cgaacccgtc ctgcacctcg gcgctgtcga ccagctgata gccgggatag accttgggca
     2401 ggcagcccat gtcgcaggcg ccctgcacgt tgttctgccc gcgcagcggg aagatgccgg
     2461 caccggggcg gccgatctga ccgcacacca gcgccaggtt ggagaccgcc accacattgt
     2521 tggtgccgct ctggaactgg gtgatgccca tgccgtaggc gatgaaggcg gccttggcat
     2581 gactcaccgc atacgccagc ttctccagtt cggccaccgg cacgccggtc tgcgcactgc
     2641 tcagctccgg cgtcaggctg gcgacatgct ggcgcagcgc ctcgacgttc tcgcagcgcg
     2701 cctcgaggaa ggcgcggttc tcccagccat tggcgaagat gatctgcagc aggccgttga
     2761 gcagggcaat gttgctgccc agcttgagcg ggatgtgctg gtcggccagc cgggcgaggc
     2821 gcgtctgccg cggatcgacc accaccagcc gtgcgccgcg ggcctgggcg cgcaggatgc
     2881 gcgcgccgat catggcgtgg ttttccgtgg tgtcggtacc gatcagcagg atcaggtcgg
     2941 cagcgtcgac atcggcgatg ctgccggtca tcgccgcgga gccgagggtc agcgacaggc
     3001 ccgccaccgt gggactgtgg cagatgcgtg cgcagtggtc gatgttgtgg gtgccgagca
     3061 ccgcgcgggc gaatttctgc gcggcgtagt tgtcctcgtt ggtcgcccgc gcacagctga
     3121 tcacccccac cgccgtcggg ccgccctcga ccagcgcctc gccgaaggct gcggccaccc
     3181 gcgccagcgc ctcgtcccaa ctggccggac gaaagccccc cgcttcgcgg atcagcgggc
     3241 gggtcaggcg gtcggccgtg tcgatggcga agctggtcga ccagcccttg ctgcacagct
     3301 ggccctggct gaccgggtgg cccggctgcg gctcgacgcc gatcacccgc ccgcgctcca
     3361 cgcgcagccc catgccacag cccaccccgc agaacgtgca aatgctcgga accacctgca
     3421 tcgccggccc tcccgccatg aaatcctgtt gaccctagac cgcctgacca ctcgcgaact
     3481 tgatcatgat caggaacttg cattgttcgc caatggccta gactggtgtc tagttcatcg
     3541 cttcgccagc agtccgggag aacgtcctcg atgcttgaga acagtggtct gcagccttcc
     3601 ggccggtgcc agcgcgagcg catcagttgt cgcgactgcg cgatccgccc gcattgcttt
     3661 ccggccaacc tgcacggcag cgagctgcag caactcgacg acatcatcca gcgcagccgg
     3721 ccgctgcaca aaggcgagca cctgttcgct gccggcgaat ccttgcagca actgcatgcc
     3781 gtgcgcaccg gcaccctcaa gacctatctg ctcagcagcg agggcgagga gcagatcacc
     3841 ggctttcacc tgcccggcga tgtgctcggc ctcgacggcc tgggcgcctt cagccacccg
     3901 agcttcgccg tcgccctgga aaccagcatg gtctgcagca tcccgctgac gcgcctggag
     3961 gagctggccg gcagcatccc gcgcctgcgc gccgagttgc tgcgggtcat gagcgcagcc
     4021 atccatgccg agcacgaaca actgcgcctg acccgcaaag gcgccgaaga gcgcctgggc
     4081 acctttctgc tcgagctctc gcagcgcttt cgccagcgcg gctattccgc gcagaacttc
     4141 atcctgccga tgagccgctg cgagatcgcc aactatctcg gcctgaccag tgaaaccgtc
     4201 agccgcctgc tcgcccgcta ccgcgaccgc gggttggtgg ccatcagcgg ccgcgaagtg
     4261 cgcctcaccg acatccgccg cctgcagcag ctgtgcggac tgggcgcggc cgaactggcg
     4321 gatggcccgg cggcatgagg cttgatcaat atcaaggtgc cactcccggc gctactggct
     4381 actagccact aaagttgtat gcaccgtcca agtcggaccc aatagggaga aaaccaagat
     4441 ggctcaaccc gaaaacaaaa ccgaaaacca gatcccggtg caaaccgaag acaaggcggc
     4501 cccgcaaaat cccctcgcca ccgccctgcg cccgctggag aacctgcggc gccaggtcga
     4561 tcgtctgttc gaggatttcg accgtggctg gcatctgccg tttgcccgca acaccgcctt
     4621 cgaccgcgaa cccttctggc gccgcgagct gagcttttcc ggcatgccgg cggtggatat
     4681 cgtcgagaag agcgacgcct tcgagatctc cgccgaactg ccgggcatgg acgagaagaa
     4741 catcgagatc aagctgagca acggcaccct caccatcaag ggcgagaaga aggacgagcg
     4801 cgaggagaag aagaaggact actacctctc cgagcgccac tacggctcct tccagcgcac
     4861 cttcggcctg cccgagggcg tggatccgga gaagatcgag gcacacttca gcaagggcgt
     4921 gctgaccctg catctgccga agaagcccga cgcgcagaag ccggaaaaga ccatcccgat
     4981 caagaccagc tgatcgatcc cgcaagccct gcctcgcgca gggctttctt taccgccgcc
     5041 ttcggccttt cgggtatcgc cgccatgtca gcgctacacc ctttccgcac agcggcgagc
     5101 ccgcctgacg gggcagatca cccggcctac tggagccaac cggccagcga gctgctggcc
     5161 gaactgggca gttccgcgca gggtctcagc gcggcgctgg cggcccagcg cctggccgcc
     5221 aatcgcccgc cactggccga gcgagcgccc ggcttctcgc gcctgctgct ggagcagttg
     5281 cgcagcccgc tggtgctgat cctgatcttt ggcgccgtcg tcgccctggt ggtacgggac
     5341 tggctggatg ccgtgatcat cctcgccatc gtgctgtgca gcgctctgct cggcgcctgg
     5401 cacgaacgtc gcgcctccca tgccatcgaa caactgcgcc agcgcatcgc cgtgcgcgcg
     5461 caggtgcgcc gcaacggcca ggtacaagag attcccgccg ccgaggtggt gccgggcgat
     5521 gtcgtcgagc tgtccgccgg cagcctgatc ccggccgatg gcgtgctgct ggaggcgcac
     5581 cactgtttcg tcagccaggg cctgctgacc ggggaaacct tcccggtgga gaagcagccg
     5641 ggcacggtcg ctgccggcgc cgccctggcc gagcgcaaca actgcctgtt catgggcacg
     5701 tcgctgcgca gcggcagcgc cagcctgctg gtggtccgca gcagcaccga cagcgagttc
     5761 ggccgcattt ccgccagcct ggcgctgcgc cccccggaaa ccgagttcga gcgcgggctg
     5821 cgccacttcg gcaccctgct gctgcgggtg atgctggtga tgaccgtgct ggtgctgggg
     5881 gccaacatcc tgctgcagcg cccgaccatc gacaccctgc tgttcaccat cgccctggcg
     5941 gtgggcctgt cgccggagct gctgccggcg atcctcagca tcaccctggc caagggcgcg
     6001 cagcgcatgg cccaggccgg agtgatcgtg cgtcacctca acgccatcga gaacctcggc
     6061 tcgatggacg tgctgtgcac cgacaagacc ggcaccctga cccgtggcgt ggtgcagctg
     6121 gacggcgcgc tggatgccac cggcaatccc gacgccggcg tgctgcatct ggcctggctg
     6181 aatgcgcatc tgcagagcgg cctgcgcaac ccgctggacg aggccatcgt tgccgccgcc
     6241 ggcaagcagc cgaacgatta ccgcaagatc gacgagatcc cctacgactt cacccgcaag
     6301 cgcctcagcg tgctggtccg cgtcggcgac gccggcccgc tgcagaccat caccaagggt
     6361 gccctggacg gcgtgctgga ggtttgcagc tgggtgcgca acggctcgcc ggacaagccg
     6421 ctcgacggca ccaccctggc cgccatccgc gaccgctatg cgcagtggag cgccaagggc
     6481 tatcgggtgc tcggcctggc ctgcaagacg atggccgccg acgcccggaa ggccgaggaa
     6541 agcggcctga ccttcgtcgg cttcctgctg ttcttcgatc ctcccgagcc cggtgtgcaa
     6601 cagaccctcg ccgccctgca ccgcctcggc gtgcaggtca aggtcatcac cggcgacaac
     6661 cagctggtgg cgcggcacgt cgccgaagcg gtgggcatcg ccgtggagcg catcgtcacc
     6721 ggcgccgagc tggcgcagat gaaggacgag gcgctgctca acctggcgcc gcgggtggca
     6781 ctgttcgccg aggtcgatcc gaaccagaag gaacgcatca tcctcgccct gcagaagacc
     6841 ggccatgtgg tcggctttct cggcgacggc atcaacgacg cgccggccct gcactgtgcc
     6901 gatgtcggca tctcggtcga caatgcggtg gatgtggcca aggaagccgc cgacttcgtg
     6961 ctgctcaagc acgacctcga cctgctgcgc cagggcatcg acgagggccg gcacaccttc
     7021 gccaacaccc tcaagtacat cttcatcacc accagcgcca acttcggcaa catgatcagc
     7081 atggcggtgg cctcgctgtt cctgcccttc ctgcccctgc tggccaagca gatcctgctc
     7141 aacaacttcc tctccgacat cccggcagtg ggcatcgccg gcgatcgggt cgaccgcgag
     7201 tgggagcgca cgccacaccg ctgggacctg cgcatggtgc gcaacttcat gatcggcttc
     7261 ggcctggtca gcagcctgtt cgacctgctc accttcggcg tgctgctgtg gttcgccggc
     7321 gaggtcgccg agctgttccg caccggctgg ttcgtcgagt cgctgctcac cgaaatcctg
     7381 atcatcttcg tggtgcgcac ctggaagccc tgctggcgca gccgtcccgg ccgcctgttg
     7441 ctgggcagct cgctggcgat catcgccgtc accctcgccc tgccctacag cccgttggcg
     7501 gtctggttcg gcctggtgcc gctgccaccc acgctgctgc tggccctgct cggtatctgc
     7561 ctgctgtacg ccggcgcctc cgagctgttc aagcactact tctaccggca ccaccagcat
     7621 ccctccggtc gcgcccaccg ccagcgcgga cagccgccgc cggcgcgacg ccgatagcca
     7681 cgaaccgatc gggggacctc tcgaaatgcg ggtggtgatc tttaatcaat cagtgacgac
     7741 caccgcaaag cccaaggcaa aggaggcgca ccttgccggc tccgcacgaa ctgaccgccc
     7801 aacagctgta ccacgcctgc gacgtggcgg agctgggctg cgacagcacc agcgacctgc
     7861 ccgacctgga ggccagcctc ggccaggagc gggctctggc ggcgttgaac ttcggcgtcg
     7921 gcatccggca cgagggctac aacctgtatg tgatgggctc gccgggactg ggcaagcacg
     7981 ccatcgtccg tcacctgctc ggtcagcagg ccgcgcagat cgaaccgccc agcgactggt
     8041 gctacatcaa caatttccgc accccgcaca agccccgcgt gctgcgcctg ccagccggca
     8101 ccggcagcgg cttgcaagcc gatatgcgcg cgctggtgcg cagcctgctc accgctctgc
     8161 cggcggcgtt cgacagcgac gactaccgca acgccatgca ggcgctcaag gacaagttca
     8221 aggcccgcga ggaggaactg ttcggcagcg tcgacagcca ggcccgcgcc agcgggatca
     8281 ttctgctgcg caccccggcc ggcttcaccc tggcgccgct caaggacggc gaggtgctgg
     8341 atgccgaggc cttcgacaag ctgtcagccg aggagaagca acagatcgag gaacacaccg
     8401 agcgcatccg ccaggtactg cgccaggccc tgcagcaggg cgccgccgcc agccgagagc
     8461 acgaccagca ggtcgagcag ctcaacgagg cggtcacccg tgccacggtc gagcgccagt
     8521 tcatcgatct ggaagcgcac tactcaagac tcgccgaagt cgctgccttc ctcgccgagg
     8581 tcaagctcga catcgtcgag aaggggctgg agatcttcgg ctcggcgccg gcggagaaac
     8641 tgctcgaccg ccagcgcctg accccgttca accgctactt cgtcaacgtg ctggtggaca
     8701 acggcggcag caagggcgcc ccgctggttt acgaggacta cccgagctac cagaacctga
     8761 tcgggcgcat cgagcaccag gcgcacatgg gcaccctgca caccgacttc actctgatca
     8821 agggcggcgc cctgcaccgc gccaacggcg gctacctgct gctcgatgtg cgcaagctgc
     8881 tgaccacgcc gttcgcctgg gaggcgctca agcgcgcgct gcaggcccgc gagctgcgca
     8941 tccagtcgct ggaacagatc ctcagcctgg ccagcaccat ctccctggag cccgacccca
     9001 tcccgctgga ggtgaaggtg gtgctgatcg gcgaccgcct gctctactac ctgctgaccc
     9061 agtacgatcc cgagttcccc ctgctgttca aggtcgaggc cgatttcgcc gaggacctgc
     9121 cgcgcagcgc ggacaacacc cggctgtacg cgcagctgat cgccaccctg cagcgccgcg
     9181 agcaactgcg tccgctggac ggccccgcgg tggcgcggct gatcgagcag gccgcgcgcg
     9241 aagcagctga cggcgagaag ctgtcgctgc acctgcagga cctgctcgac ctgatgcgcg
     9301 aggccgacta ctgggccggc cagcagcagc ggacgcaggt gcgccgcgag gacgtcgaac
     9361 aggcggtgcg cgaacgcctg caccgcgccg accagttgcg cgagcgcctg ctcgaggccg
     9421 ccctgcgcga cctgcgccac atcgacaccg atggccagtg cgtggcgcag atcaacggcc
     9481 tggcggtgct gcaactgggc gcccacgcct tcggccatcc ggcgcggatc agcgccacgg
     9541 cgcgcctggg cgacggcaag ctgatcgaca tcgagcgcga ggtggaactg ggcggcgcgc
     9601 tgcactccaa gggcgtgctg atcctctccg cctacctggc cagccgcttc ggcggcagcc
     9661 agccactgtc gttctcggcc agcctggtgt tcgagcagtc ctatggcggc atcgatggcg
     9721 acagcgcctc ggccgccgag ctgtgcgtgc tgcagtcggc gctggccgac ctgccgctgc
     9781 gccaggacct ggcgatcacc ggctcggtgg accagcacgg gcgcatgcag gtgatcggcg
     9841 cggtcaacga gaagatcgag ggcttcttcg acctgtgcgc cgcccgcggc ctctccggca
     9901 gccagggggt gatcatcccg cacggcaaca tcaagcacct gatgctgcgc cgcgaagtgg
     9961 tggccgcggt ggccgccggg cacttccacg tgcacgcggt agagcacgcc gagcaggcca
    10021 tggcgctgct caccggcctg cccgccggcg aggtcgacgc ccagggccag ttccccgccg
    10081 ggacgatcaa cggcgcagtg cagcggcggc tgcacgagtt gagcgagctg cgtcagcgct
    10141 atgctcatcc acagtcagac ggccctgccg gcaacgggca ggccgatatg tgaaccgagg
    10201 gagataccca ctccgaagtc cggcggcggg cttaccgccg caaggtaggc agccatggcc
    10261 agcgtggaaa acccgctcag cctgttcctc ctcggcgatg tcatgccggc ccgtggcatc
    10321 gaccagatcc tgccgcaccc cggcgatccg cgactgtacg aagactacgt gcacgacgcc
    10381 cgcgactacg tggtgctggc cgagcagcgc tgcggtccca tcgagcggcc ggtggacttc
    10441 gcctacgtct ggggcgacgc cttgggcgag ctgcaacgcc gccagccaca gatacgcctg
    10501 atcaacctgg aaagcgcggt gacccgccac ggccggccgg agcccaaggg aatcaactac
    10561 cgcatgaccc cggagaactt cccgctgctg cgcgccgccg ggatcaactg ctgcaccctg
    10621 gccaacaacc atgtgctgga ctggggtgag gtcggcctgc gcgacaccct ggataccctc
    10681 acggcccatg gcatgccctg ggccggcgcc ggccttgacc gagccagtgc cgaagcaccg
    10741 gccgtgctgg cgctgccgga tgggcggcgc ctactggtat tcgccttcgg tctgccggac
    10801 agcggcatcc ctgcgcactg ggccgccggt ccacaccagg ccggcgtggc gcggctggac
    10861 gacctgtcgc cacgcagcct ggcccatgtg gccggcctga tccacgcctc gcggcagacg
    10921 ggcgaccgcg tgctggtatc gctgcactgg ggcgacaact gggacttcgc cgtccctgcc
    10981 gaacaccgcg ccttcgccca cggcctgatc gaccaggccg gtgtcgacct ggtcaacggc
    11041 cactcctcgc accacatcaa gggcatcgag gtgcaccgtg agcggctgat cctctatggc
    11101 tgcggcgacc gcctcaacga ctacgaaggc atcgagggac acgcggactt tcgcggcgaa
    11161 ctggggctgc tgtatttcgt ccgcctgcat gacgacggcc gcctgctcgg cctggacatg
    11221 gtgccgacgc gcctgcagcg cctgcgcatc aacctggccg aaggcgtcga ccgccgctgg
    11281 ctgttcgaca ccctcgcccg cgagtgcgcc accttcggcg gcggcattcg tcagcagatc
    11341 gacggcagct tcgccctgac ctggccacgg ggcgcgccat gagcgtgagc aacgccgaga
    11401 tcgccgccat cttcgaggaa atcgccgaca ttctcgaact cgaggccgcc aacccgttcc
    11461 gcatccgcgc ctaccgcaat gccgcgcgca ccctgcaggc cttcgccccg gacatcgccg
    11521 gcctgcttgc cagtggccag tcgctgccca agctgccggg catcggcgag gacctggcgg
    11581 ccaagatcgg cgaaatcgcc gccaccggca gttgcgccat cctgccgcgt ctgcgcagcg
    11641 aagtaccggc cggcctgcgc gaactgctca aggtatctac gctggggcca cggcgggtcg
    11701 cccgcctgtg gcacgaactg ggcatccaga ccccggccga gctgcagcag gccgcccgcg
    11761 ccgggcgcat ccgccagctg cacggcttcg gcgagcagac cgaggcgcgc ctgctgcaag
    11821 cggtcgaact gctgctgcat actccgcagc gctggctgct gagccgggtc gcgccgattg
    11881 ccgaacggct ggtcgccgag ctgggcaagg tgcccggggt gatcgaggta gcgagcgccg
    11941 gcagctttcg ccgcggccgc gataccgtcg gcgatctgga cattctggtc aacgcacggc
    12001 gcggcagtgc ggtgatccag cgcttcacca gcgatgccga ggtcggccgc gtgctggccg
    12061 ccggcaccac ccgggccagc gtgttattga actctggcct gcaggtcgac ctgcgcctgc
    12121 tttcaccacc ggcctggggg gccggcctgc tctacctgac cggcagcaag gcgcacaaca
    12181 tcgccctgcg gcgcatggcc caggaacgcg ggctgaagat cagcgaatac ggcgtctatc
    12241 gcggcaagca gcgcatcgca ggcagtagcg aggactcgat gtaccaggcc ctcggcctgc
    12301 cgtggatcgc ccccgagctg cgcgaggacc gcggcgagat cgaggcagcg ttggccggca
    12361 cgctgcccgc gctggtggaa ctgggtgatc tgcgcggcga cctgcacgcc catacccgcg
    12421 ccagcgacgg gcacgacacg ctggaggaaa tggccgccgc cgcccaggcc gccgggctgg
    12481 agtacctggc gatcaccgac cactccagcc gcatggccat cacccacggc ctggatgccg
    12541 acgggctggc ccggcagatc gagcagatcg acgcgctcaa cgaaagactc tccggcatca
    12601 ccctgctcaa gggcatcgag gtggacatcc tcgaggacgg cagcctggac ctgccggacg
    12661 ccatcctcgg ccgcctcgac ctggtggtcg gcgccgtgca cagtgccttc aatctctcca
    12721 aggaacagca gacccgccgc ctgctgcgcg ccctggagca ccagcacttc agcatcctcg
    12781 cccaccccag cgggcggctg ctgggcgagc ggccgccgat caactgcgat ttcgccgccc
    12841 tgctcgccgc cgctcgccag cgtggctgcg cgctggagct caacgcgcaa gccgaacgcc
    12901 tcgatctcga cgacctgcac tgccgcgagg cccgcgatgc cggcgtgccc atcgccatca
    12961 gctccgacgc ccaccgcacg ctggatttcg ccgggctgcg cttcggcatc ggtcaggccc
    13021 gccgcggctg gctggcggcg aaggacgtcc tcaacacccg gccactggct gagctgcgcc
    13081 gctggctcaa gcactgagac cgcccctcgg ccggtgcgct gcgccatgaa aaatgccgct
    13141 cacccatgac aggcgagcgg catcttcaga acaaccgcga gtgggagtca gccgatcagg
    13201 ccgacagttc caccagcagc ttgttcaggc gctgcacgta ggcggccgga tccttcaggc
    13261 tgtcgccggc agccagcgct gcctggtcga agaggatgtg cgacaggtcg gcgaagcggc
    13321 tctcgtcggc ctcggcatcc agcttctcga tcagcgggtg ggccgggttg atctcgaaga
    13381 tcggcttgga ttcgggcacc ttctgcccgc tggcttcgag gatctggcgc atctgcaagc
    13441 cgagatcctg ctcgccgatg gcgaggatcg ccggcgagtc ggtcaggcgg tgggatacgc
    13501 gcacctcggc cacctgctcg cccagcgcct tcttcagccg ctcgaccagc ccttccttgg
    13561 ccttggccac ctcttcctgg gccttcttgt cctcctccga gtccagcttg ccgaggtcca
    13621 gatcgccgcg ggcgacgtcg atcagctgct tgccgtcgaa ctcggtgagg tagctcatca
    13681 gccactcgtc gatgcggtcg gtgagcagca gcacctcgat gcccttcttg cggaacacct
    13741 ccaggtgcgg gctgttcttc acctgggcat agctctcgcc ggtgaggtag tagaccttgt
    13801 cctggccgtc cttcagacga ccgaggtagt cggccagcgc cacactctgc tcgccgctgg
    13861 cgtcggaagt cgacgcgaag cgcagcagcc cggcgatctt ctccttgttg gcgaagtctt
    13921 ccgccggacc ttccttgagc acttggccga aggccttcca gaaggtctgg tagtcgtccg
    13981 gcttgtcctt ggccagcttc tccagcatgt ccagcacgcg cttggtcagc gccgacttca
    14041 tcgagtcgat caccgggccg gactgcagga tctcgcggga gacgttcagc gacaggtcgt
    14101 tggagtcgac cacgcccttg atgaagcgca ggtacagcgg caggaactgg tcggcctggt
    14161 ccatgatgaa cacgcgctgc acatagagct tgaggccctt gggcgcttcg cggtggtaca
    14221 ggtcgaacgg cgccttgccc ggcacgtaga gcagcgaggt gtactcgagc ttgccctcga
    14281 ccttgttgtg gctccaggac agcgggtttt cgaagtcgtg ggcgacgtgc ttgtagaact
    14341 cctggtactc ctcgtccttc acctcgctgc gcggacgggt ccacagcgcg ctggcgcggt
    14401 tgacggtttc ccactccacc tcggccggct tgtccttctc ctcaccgaag tgttccttgg
    14461 gcagctcgat gggcagggcg atgtgatcgg agtacttctt gattatgttg cgcaggcgcc
    14521 agccgtcggc gaactcggtt tcgtcggact tcaggtgcag gacgatgcgg gtgccgcgct
    14581 cggccttgtc gatggcggcg atgtcgaact cgccctcgcc cttcgagctc cagtgcacgc
    14641 cctcgctggc cggcaggccg gcgcggcgac tgtagacgtc gacctggtcg gcgacgatga
    14701 acgccgaata gaagccgacg ccgaactggc cgatcagctg cgaatccttc ttctggtcgc
    14761 cggagaggtt cttgaggaag tcggcggtgc cggacttggc gatggtgccg aggtgcgaga
    14821 tgacatcgtc gcggctcatg ccgatgccgt tgtcgtcgag ggtgacggtg cgggcgtcct
    14881 tgtcgaagct gacgcggatc ttcagcgggt cgccgccttc gagcagttcc ggcttggcca
    14941 gcgcctcgaa acgcagcttg tcggcggcgt cggaggcgtt ggagatcagt tcccgcagga
    15001 agatctcctt gttcgaatac agggaatgga tcatcagctg aagcagctgc ttcacttcag
    15061 tctgaaaacc cagggtctct ttgcgagtct ccacgctcat gaatgcgaac tccacggatg
    15121 acaggccgcg tgtgcggcga atgccatgga tatggagact tcgtccggga tttcaagggc
    15181 ttggcacccg cggccggaaa atcagggctc cagtagctcg atctgcgtga cttcgctgga
    15241 gcgcagcagg aagctggcct cgccctgccc gcccagggca tgctggatca ccagccgccc
    15301 ctccggattg atgccgtcga agcgcccctc cgcgctgcgg ccgcgctcgg tcatcacccg
    15361 cacccgcgcg cccaggtaac gccccggacg atccagcaga ctctcgaggg aaaaaccgat
    15421 acgccggtcg agcggccgct cctcaccacg caccaccgtc gtcggcaccg gcgccgtgcg
    15481 cagcaggtcg tcctcgctgg gcggcggcag ggccggcgcc tgatcgccct gtacccgcca
    15541 gccatgcagg tcgctgcgcg cgagggtgag cggcatccgc ttggccactc cggcaatgcg
    15601 cgcctgcacg tcgagatcga gctggtggct gtccagatcc aggggtggta gcggggcgag
    15661 ttcgacagcc cgggtccgcg cattgcgctg cagatagtcg acgatcagcc actcgagggt
    15721 tgcctgcgaa ttgtgccggg tgtcgacgcg tccgttgcga taacgcagat gactggtata
    15781 gagctccacc ttgccactca gcgaggtgcg gtagccggac ggcagcacca gatggaagtc
    15841 gccacgcagc aggttcggct cggcactctt gaggtccagg tgcagggtgt agccactggt
    15901 gatccgtcgc gtgtcgggct gatcgtggtc gggcagcagc cagctgagtt cgatcgccgg
    15961 caggttgtcg cgatcctcgg gcagcacgtt gagctgcaga tcgccgctcg gctggctggg
    16021 caggcggatg ctcagctcgc ggcgggcgaa gaaatcgtcg ccttcgcgca ggatcagggt
    16081 gccgtcgatc aactcgccca gttcgggggc gaagggcttg ccgttgaatt cgccgcgcag
    16141 ctggatgtcc agttcgcggg ccaccttggc ctccggttgc agccagcgca ggctgaggat
    16201 ggcggcgaga cggtcggcgt cgtggctggc cagcagggtc aggccgacca ccagaggaat
    16261 acagcccatg cccatcagcg tcaccgagct gcgcgcgcga cgccagtgac gcacgatgaa
    16321 cagcaagggc agtggcggca gcagactgcc cacgccccac agcaggctgg tggaaaaggc
    16381 ctgcaccacc aaccagacca tcccaaacag gatcagcagc aggccaccga cgatcagcag
    16441 agcatccatc agacgaacat ccacaaacga cgaaaacaaa ggaaggcgcg catcacgact
    16501 cttcaacctt taagtgttga tggtgacgga tgatacccgc cagcttcgac cggccaaagt
    16561 ggccattact cacaaatgct cgacccgccg aacttttatc cgtcatgaca ctccaatagt
    16621 gtgattgaag tattcagtga aatccgactg ggttcatgaa ctgcatgaat ctccatcgga
    16681 tgactggccg taggaatacc ggttttgcaa aagcgccaat gctgcgctac atacttaatc
    16741 ggtactcgca atcctcatga catggacact ccctaggagc gacaaaatgc aaaaaccgct
    16801 gacctacgcc ctgctgctcg ccgccaccct cggtctggcc gcctgcggcg accaagccga
    16861 caagaaagcg gaatcggcag ctggctcggc cccgcaacct acccagtcga tgggcgaggc
    16921 tgccaagaag gccactgatg ccgcgaagga ggccggtgcc gtcgtcgagc agaaggctgc
    16981 cgaagtgacc gagcaagcag ctggcgccgt gaaagacgcc ggcgctgccg tcgaacagaa
    17041 agccgctgaa gtccagcagg aggctggcaa ggtcgaagaa gagaagaaat aaggccctga
    17101 cacaaaaacc gccctgccct gcccgaagaa acggtgcagt ggcagggcgg ttttttttgc
    17161 ctgcagaaaa ggaccggccg ctatctgcgc gcgatcggga gtggtacttg tcccctgccc
    17221 ccgctttcta tccgccctgc tgcttcgccg actggcccaa ctcgaccggg ctgtagacca
    17281 tcacggccag cacatccgag tgaaattcgc ggcgatacag gacaaatacc actccagcgg
    17341 tggccagcat gtagaaccag gggttgatga accaggccag ggtcgccaga ccgaaatagt
    17401 aggaacgcag accgaagttg aactggttgg ccgccatcga gatcacccgc gccgtgcgct
    17461 cggcatagga cttgcgctcc tgctcggtga cccgctcctc ggtgagcatt ggtgccgcgc
    17521 cgaccatcat ggccgcaaag ttgtactggc gcatgcacca ggtgaaggtg aagaaggagt
    17581 agatgaacac caccgccaca gcaatgatct tgacctcggc gatctcgcga ctgaccgcct
    17641 gcacgaaggg caattcggcc agcaccgaca ccgcccgctc ggtggagccg agcacggtga
    17701 ggataccggc gaggatgatc agggtgctgg aggcgaagaa cgacgcgttg cgctccaggt
    17761 tgccgatcac gttggcgtcg gcgatgcggt tgtcgcgcat cagcaggcgg cgcatccagt
    17821 cttcccggta caggtgcagg acgctggcca ggcagggcgt gctcccgccc ttccagatcg
    17881 cgtagcgggt gtacccccac caacacagga cgaaccagga aaccgcgatg aagtggggca
    17941 agtgttgctc gaatggcatg acaactcctt gaaagtccgg gcaattatat ctggccgaaa
    18001 taccgcggtc agcgcccgaa acaaccacgc cctgcgctcg cgagccggcg aagtgacagc
    18061 tgcccacatg aactagcgag ctcaggcctg cggccggccc agcgcccggt cgatcaccgc
    18121 caccagcagc agcgtggcca acaccggcaa cagccagccc aggctgtgct cggccagcgg
    18181 caggcgagcg aggaaagccg gcactgccgc cttgaatcct gccgcgacca acccatcgct
    18241 tacgccgaac accagggcca ccgccatcac cggcacgaac acccgcgggg ggtgacgcca
    18301 cagccgatcg gcgaggctga gcagcaccag cacgatggcc agcgggtaga gaccgaccag
    18361 taccggcagc gacacgccga tcaactgggt cagcccctgg ttggccagca gcaggctgaa
    18421 caggctggtc agcaccacca ccgtgcggta gggcagcccg aactggctgc ggaagaactc
    18481 gccgcaggcg gcgatcagcc ccaccgcggt agtcaggcat gccagggtga taaccagtgc
    18541 cagcagcaac tcgcccggca tgccgaaggt gtaccgtaca taggtggtga gtatctgcac
    18601 gccgttctgc gccccggcgg cgatgccctg gctggtggcg ccgagacgga acagcgccag
    18661 atacaccagg gccaggccgg tagcggcgat cagcgcggcc aggatcgagt agcgcgtgac
    18721 ccgatgcgcc atgtcgatgc cgtggtcgcg gatagccgtg gcgatcacga tgccgaacac
    18781 cagcgcgccc agggtatcca tggtcaggta gccctggaga aagccctgca gcagcggcgc
    18841 ctcccgatag gcaggagcaa tgctgttgcc aacctcgccg gcgggagcga acaccgccgc
    18901 gcctcccagc aacagcaggc cgataatcag taccggggtg atcatcttgc cgatgcgctc
    18961 gagcagccgg cccggattga ggacgagaaa cagcacggcg gcaaaatacg ccagcgtata
    19021 cagccccagc ggcagggggg cgttgccggt gaaggacgcc acgcccatct cgaacgacac
    19081 caccgcggtg cgcggggtgg cgaacagcgg gccgatggcc agatacaccg ccacggcaaa
    19141 tagcagcccg gcgcgccgcc ccaggggcgc agtgagccgc gccatgccgc cacctacccg
    19201 cgccagtgcc accacggtaa gcagcggcag accgaccgcc gtgagcagga agcccagcgc
    19261 cgcactccac accgatgcgc cggcggccat gccgacactc ggcggaaaga tgatgttgcc
    19321 cgccccgagg aacagcgcga aggtcatgaa gccgagaccc aggacgtcgg cggttttcag
    19381 atgattcatg cactcaacca gatagagaag tcgtgacccg gcaggagccg gaaacggaag
    19441 gagccacccg caggtggctc cttccgtcag gcctgcagca gcggatccta tttcatggtc
    19501 cagccagtcg cttcggcaag cgccttgccg atgtcggcca gcgagcgcac ggtcttcacc
    19561 ccggcgtcct gcagcgcggc gaacttctcg tccgcggtgc ccttgccgcc ggaaatgatc
    19621 gccccggcgt ggcccatgcg cttgccggac ggtgcggtca cgccagcgat gtaggacacc
    19681 accggcttgc tcacgttggc cttgatgaag gctgccgcct cttcctcggc actgccgccg
    19741 atctcgccga tcatcacgat ggcttcggtc tgcggatcct gctcgaaaag cttgaggatg
    19801 tcgatgaagc tcgagcccgg gatcggatca ccgccgatgc ccacgcaggt ggactggccg
    19861 aagccggcat cggtggtctg cttcacggcc tcgtaggtca gggtgccgga gcgcgacacg
    19921 atgccgacct tgcccggcag atggatgtgg ccgggcatga tgccgatctt gcactggccc
    19981 ggggtgatca ccccggggca gttcggcccg atcaggcgca cgcccagctc gtcgcacttc
    20041 accttgacct cgagcatgtc gagggtcggg ataccctcgg tgatgcagat gatcagctgg
    20101 atgccgccga aggccgcctc gaggatcgag tccttgcaga acggcgccgg cacgtagatc
    20161 accgaggcgg tggcgcccgt ggcggccacg gcgtccttga cggtatcgaa caccggcagg
    20221 ccgaggtggg tggtcccgcc cttgccggga gtcacgccgc cgaccatgcg ggtgccgtat
    20281 tcgagggcct gctccgaatg gaaggtaccc tgactgccgg tgaagccctg gcagatgacc
    20341 ttggtgtcct tgtcgatcag gatgctcatt gcttgccctc cgcggccttg acgaccttct
    20401 gcgcggcgtc ggtcagactg gttgccgcca tgatgttcag accgctttcc gccagcaccc
    20461 cggcacccag ttcggcattg ttgccttcca gacgaaccac taccggcacc tgcacaccga
    20521 cttcgcggac cgcaccgatg atgccttcgg cgatcatgtc gcagcgcacg atgccaccga
    20581 agatgttgac cagcaccgcc ttcacgttgc tgtcggagag gatgatcttg aacgcctcga
    20641 ccacccgttc cttggtcgcg ccgccaccga cgtcgaggaa gttggccggc ttgccgccat
    20701 gcaggttgac gatatccatg gtacccatgg ccaggccggc gccattgacc atgcagccga
    20761 tgttgccgtc cagcgccacg tagttgagct cccacttggc cgcatgggcc tcgcgcggat
    20821 cgtcctgcga ggggtcctgc atctcgcgca gcctgggctg gcggtacagg gcattgccgt
    20881 cgatgttgag cttggcatcc aggcagtgca ggttgccgtc ggccttgatc accagcgggt
    20941 tgacctcgag cagcgccagg tcgtagtcct cgaacagctg ggccagaccg acgaagatct
    21001 gcgcgaactg cctgacctgg tcaccctgca ggccgagctg gaaggccagt tcgcggccct
    21061 ggaacggctg ggcaccgacc agcggatcga tgctgacctt gaggatcttc tcgggggttt
    21121 cgtgggcgac tttctcgatg tccacgccgc cctcggtgga ggccatgaac acgatgcgcc
    21181 gcgaggagcg gtcgactact gcacccagat acagctcctt ggcgatatcg gtgcaggcct
    21241 cgaccagaat cttgctgacc ggttgcccgt tggcgtcggt ctggtaggtc accagacgct
    21301 gatccagcca ctgctgggca aaggccttgg cctcttcctt gttacgaacc agcttgacgc
    21361 cgcccgcctt gccacgaccg ccggcatgca cctgggcctt caccacccag gcagtgccgc
    21421 cgatcttgtc acaggcttcg gctgcctctt tcggagtatc aactgcatag cctttggaga
    21481 ccggcagtcc atactcggca aacaactgct taccctgata ttcgtgaaga ttcatgtttc
    21541 cataccactt tagttgtaag tattggcatc atggatctta agcgggaaag tgttgcagtg
    21601 actagagtgc gtagcacgga tttccgcgac cgtcacgtga ggcagaaatg aaaacggccc
    21661 cggtcgcggg aagtccaaga ctccctcgac cggggccgct ggggccgcac gtctgaagcc
    21721 aggcttcaga cgtgcggcag gcgcggatta gcgcttcttg cggttggcaa cgtgaatggc
    21781 atggccattc actgccaggg cagcttcatg cagcgcctcc gacagggtcg ggtgcgagaa
    21841 caccatcatg cccaggtctt cggcactggt gccgaattcc atgccgatgg cgccctgctg
    21901 caccagctcg gcagctgccg ggccaatcac gtgcacaccg agcacgcggt cggtgttggc
    21961 atcggcgatg accttgacca gaccgtgggt atcgttggca gccatggcac ggccgctggc
    22021 ggcgaacggg aaggtgccga cgttgatggc aacgccttcg gctttcagct gctgctcggt
    22081 cttgccgacc gcggcgattt ccgggtgggt gtagatgacc gacgggatca ggtcgtagtt
    22141 cacctgcgcc ttgtggccgg cgatgcgctc ggcaaccatc acgccctctt ccgaggcctt
    22201 gtgggccagc atcgggccac gcaccacgtc accgatcgcg tacacgcccg gcacgctggt
    22261 ggcgcactgg tcgtcgacga agatgaagcc gcgctcgtcc agggtcacgc cgctgtcggc
    22321 agccagcacg tcctgggtca ccgggcgacg gccgaccgcg acgatcagct tgtcgaaggt
    22381 cagctgctgc tcgccggcag cgtcggtgaa gtgcacagtg acctgcttct tcttcacttc
    22441 cgaaccggtc acgcgggcat tcagacggat gtccaggccc tgcttggtca gcagcttcag
    22501 ggcttccttg gcgatctgct cgtcgacagc cgcgaggaac ttgtcctggg cttcgagcac
    22561 ggtcacttcg gcacccagac gggcccacac cgagcccagc tccaggccga tgacgccggc
    22621 gccgatcacg cccagcttct tcggtacgct ctggaattcc agggcgccgg tggagtcgac
    22681 gatgatgtcg tggtcagccg gagccggcgg aatatcgatc ggcttggaac cggaggcgat
    22741 gatcacgtgg gtggcttcca gcacctggac cgaaccgtcc tcggcggtaa cttccacctg
    22801 cttgccggcc agcagcttgc cgtggccttc taccagggtc acgccgttgg ccttgaacag
    22861 gccggcaacg ccggtgttca ggtttttcac gatggacgcc ttgcgctcga ccatcgccgg
    22921 cacgtccatg ctcacgccct tgacctcgat gccatggacg ccgaagcttt ccttggcttc
    22981 gtggtacttc caggagctgt ccagcagcgc cttggacggg atgcagccga cgttcaggca
    23041 ggtaccacca aggacgacct tgccttcctt gcccttgtac ttctcgatgc aggcagtctt
    23101 caggccgagc tgagctgcac ggatggccgc aacatagcca ccggggccac cgccaatcac
    23161 cactacgtcg tatttctggg tcataactct caatcctttt gggataaacc gggcggcctc
    23221 cgtgtcagag accgccgtgg gagaaacgcg ggatcagacg tccagcaaca gacgagccgg
    23281 atcttccagc agatccttga tggccaccag gaagctcacc gcttccttgc cgtcgatcag
    23341 acggtggtca taggacaggg ccaggtacat catcggcagg atcacgacct gaccgttaac
    23401 ggccatcgga cgatcctgga tcttgtgcat gccgaggatg gcggtctgcg gcgggttgac
    23461 gatcgggctg gacagcaggg agccgaacac gccgccgttg gagatggtga aggtgccgcc
    23521 ggtcatctct tcgatggtca gcttgccttc cttcgccttg cggccgtagg cgccgatggt
    23581 gccctcgatc tcggccaggc tcatcagctc ggcgttgcgc aggaccggaa ccaccaggcc
    23641 acggtcgctg gacacggcca cgccgacgtc cgagtagccg tggtagacaa tgtcgccgcc
    23701 gtcgatcgag gcgttgacgc ccgggaagcg cttgagcgct tcggtggcgg ccttgacgaa
    23761 gaaggacatg aagcccaggc gcacaccgtt gtgcttcttc tcgaacaggt ccttgtactt
    23821 ggcgcgcagg tccatgatcg gcttcatgtt gacttcgttg taggtagtca gcatggccat
    23881 ggaggactgg gcttcgacca gacgctcggc gatcttggcg cgcaggcggg tcatcggcac
    23941 gcgcttctcg acgcggtcac ccgcggcgaa gatcggggcg tcggcagccg gagcggccgg
    24001 cttggcggcc ggagccggag cagctttctt ggcatcgacg gcggcaacgg cgtcttcctt
    24061 ggtgacacga ccgcccttgc cggtacccgg gatggcgttg cgatccagat cgttctcggc
    24121 ggcgatcttg cgtgcagccg gcgacaggat ggcttcttcg gcggcggccg gagcagcggc
    24181 agccggggcg gcagcagcgg cagccggcgc agcggcgaca gcgccttcgt tcagcttgcc
    24241 gagcagctga ccgctgagca cggtgtcacc ctcgttggcg acgatctcgg cgagaacgcc
    24301 atcgacttcg gccaggactt ccatgaccac tttgtcggtt tcgatgtcga caatcagctc
    24361 gtcacgcttg accgcgtcgc ccggcttctt gtgccaggta gcaacggtac cgtcagcgac
    24421 ggattccggg aaagtcgggg ctttgatctc gatagccatt atctgcattt cctattgatt
    24481 cggggtcttc atgggggcac ctgccggagc gggtccggca gggccacttg cctgaaggca
    24541 ttaaatggtg aaggcgtcct gcagcagttt ttcctgctgc tcggcgtgca tcgatgcgta
    24601 gccacaggcc ggagatgccg aagcatcgcg accggcgtaa gccagaccga gggtcttctt
    24661 gtacgcagtg atgacgcggc gcatgtgatg ctggctgcag taccaggcgc cctggttcat
    24721 cggctcttcc tggcaccaaa ccacgtgctt gaggttcttg tacgcggcca tgacttcggc
    24781 cagctcttct tccgggaacg gatagagctg ctcgatgcgc acgatggcgg tgtcctcgcg
    24841 accttcggca cggcgcttct ccagcaggtc gtagtagacc ttgccgctgc acaggaccag
    24901 acgctcgacc ttcttcggat cgaggctgtc gatttccggc agcaccggct ggaacgagcc
    24961 ctgggccaga tcttccagag tcgagatggc cagcttgtgg cgcagcagcg acttcggcgt
    25021 cagagccacc agcggcttgc gcagcgggcg gatcacctgg cggcgcagca tgtggtagac
    25081 ctgggccgga gtggtcggca cgcagacctg gatgttctgc tcggcgcaca gttgcaggta
    25141 acgctccaga cgcgcggagg agtgctccgg accctggcct tcgtagccgt gcggcagcag
    25201 catggtcaga ccgcacagac ggccccactt gtgctcgccg ctggtgatga actggtcgat
    25261 caccacctgg gcaccgttgg cgaagtcacc gaactgggct tcccagatca ccagcgcgtt
    25321 cggctgggtg gtcgcgtagc cgtattcgaa cgccagcacg gcttcctcgg agaggaagga
    25381 gtcgtacagc tcgaacttcg gctgaccgtc gaacaggtgc tgcagcggaa tgtggcggct
    25441 ggcgtccttc tggctgtgca gcgcggcatg gcggtgcgag aaggtgccac ggccgacgtc
    25501 ctgaccggtg atgcgcaccg gatggccttc cttcagcagg gtggcgtagg ccagggtttc
    25561 cgcgcagccc cagttgatcg gcatggcacc gacgcccatg cgaccgcgat cttcgtagat
    25621 cttggcaacc tggcgctgga ccacgaaacc ttccggaatc tgcagcagct tggcgttcag
    25681 ttcctgcagg gtcttcaggt cgaagctggt gtcgtgctgg gcagtccagg cgtggcccag
    25741 gtacggcgtc cagttgacga acagctcggc gttgggttcc ttgaccaggc tcttgaccac
    25801 gtgctggccg ttgtccagcg cggtacggta ctcctcgacc ttggcttcga cctgagcagt
    25861 gctcagggca ccggcctgca ccagggcatc ggcgtacagc tcacgggtgg tgcgctgctt
    25921 ggcgatcttc tggtacatca gcggctgggt gccgctcggc tcgtcggcct cgttgtggcc
    25981 gcgacgacgg tagcagacca ggtcgatgac cacgtcacgc ttgaactgca tgcggtagtc
    26041 gacggccatc tgggtcacga acagcacggc ttccggatca tcgccgttga cgtgcaggat
    26101 cggcgcctga atcatcttcg ccggatcggt gcagtactcg gtggagcgcg agtcttcggt
    26161 attgctgatg gtgaaaccga cctggttgtt gaccacgata tggatcgtgc caccagtctt
    26221 gaagccgcgg atctgcgaca tctggaaggt ttccatgacc acgccctgac cggcgaaggc
    26281 cgcgtcaccg tggatggaaa tcggtaccac cttgtcgccc acggcatcgc cgcgacggtc
    26341 ctgacgggca cgcacggagc cctcgaccac cggggaaacg atttccaggt gcgacgggtt
    26401 gaacgacatg gccaggtgca cttcgccacc cggggtcatg acgttggagg agaagccctg
    26461 gtggtacttc acgtcaccgg agctgaggcc ttcgacacgc ttgccctcga actcgtcgaa
    26521 caggtcacgc gggttcttgc ccagcgcgtt gaccagcagg ttcaggcggc cgcggtgggc
    26581 catgccgatg acgatttcct tggcgccgta ggagccggag cgctggatga tctcgtcgac
    26641 catcggcacc atgctctcgc caccttccag accgaagcgc ttggtgcccg ggaacttggt
    26701 acccaggtac ttctccaggc cttcggcggc agtcagacgc tcgagcaggt ggctcttggc
    26761 ttcgacggag aactccggac gaccacgcac gctttccagg cgctgggcga accagttgcg
    26821 ctgctcggca tcgacgatgt gggtgaattc cgcgccgatg gtgccgcagt aggtagcctg
    26881 caggccatcg cgaatttcgc gcagggtcgc ttcctggcgg ccgatgtaca gatcgccggt
    26941 gcggaagacg gtgtcgagat cagcgtcggt cagaccgtag tgatggatcg acagatcagc
    27001 cggagcctgg cgctgccaga ggcccagagg gtcgaggcgg gaagcctgat ggccgcgcat
    27061 gcggaaggcc tgaatcagac gcagaacttc gacctgcttc ttctcgtgct cgctgctcac
    27121 agcaccggcg gacaccggct gggcgcgacg ctgattcttg gccagcagga cgaagtgatc
    27181 gcggaccgtg gcatgcgaaa cgtcgggaga ctggttgccg ccggttggca gcttctggaa
    27241 gtaggtgcgc cactcttcgg gcacagcgtt gggatcgtgc aggaagagct cgtagagttc
    27301 ttcgacgtag gcagcgttac caccggacag gtgggcactg ttccacatcc gctgcattac
    27361 gctttcatgc atgctaggtc accctcggta aggggacacc acctggcgtg gagaaccgca
    27421 ccatggctcg aaagtcctga gcagcgactt cggtaaagcc actacggatc ccgcagatgg
    27481 ttcgggtttt aacccgatcg cccctgctgg tcgtttttta atcagaaacc gcggctttgt
    27541 gggctacggc tggctttgca actgcgctgg ccggccatcc agccagcgca ggtttcgttg
    27601 aatcaccgcc agaagcggat catcaggtac cgctttgcag cagcatgtta cgcacgtggc
    27661 cgatagcctt ggtcgggttc agacccttcg ggcatacgtt cacgcagttc atgatgccgc
    27721 ggcaacggaa cacgctgaac ggatcgtcca gcgaggccag acgctcttcg gtcttggtgt
    27781 cgcggctgtc ggccaggaaa cgataggcct gcagcagtgc ggccggaccg aggaacttgt
    27841 ccgggttcca ccagaaggac gggcaggaag tcgagcagca agcgcacagg atgcactcgt
    27901 acaggccatc cagcttctcg cgctgttccg gcgactgcag acgctcgatg gccggagccg
    27961 gagtatcgtt ctgcaggaac ggctgcacct tctcgtactg cttgtagaag atgctcatgt
    28021 cgaccaccag gtcacggatg accggcagac ctggcagcgg acgaatgacc agcttgccgt
    28081 tcttcaccac cgccgacagc ggggtgatgc aggccaggcc gttcttgccg ctgatgttca
    28141 tgccgtcgga accgcatacg ccttcgcggc aggagcgacg gtaggagaag gtgacgtcct
    28201 gctccttgat cagggccagc acgtccagca ccatcaggtc cttgccatcg gtgttgacct
    28261 ggaagtcctg catgaacgga gcagcgtctt tttccgggtt atagcgataa acactcactt
    28321 gcaacatggc ggctaccctt aataagtacg aaccttgggt tcaaaggccg gaacggtctt
    28381 cggcgagaaa ttgacaccac gcttggtcac gcgcttctca cccgggaagt acagggagtg
    28441 gcacagccag ttctcgtcgt cgcgatcctc gaagtcttcg cgggcatgcg caccacggga
    28501 ctctttgcga gcctcggcag cgatcgccgt ggcttcggcc acttcgagga ggttttgcag
    28561 ttccagcgct tcagtacgcg cagtgttgaa agcctggctc ttgtccgcga tcttgacgtt
    28621 cgcgatgcgt tcccgcagtt cggccagttg ggcaataccc ttctgcatgt attcgccggt
    28681 acggaataca ccgaagtagt tctgcatgca gttttgcagc tctttacgca gcggagccac
    28741 gtcttcgcca gtggtgcgct cgttgacgcc ggacaggcgc ttgagcgaag cctcgaggtc
    28801 ggactcggtg gcaccacggt gctcgatgcc ttccttgagg gctttttcca ggtgcagacc
    28861 agcggcacga ccgaacacca ccaggtcgag cagcgagttg ccgcccagac ggttggcgcc
    28921 gtgtacggat acgcaggcga cttcacctac ggcgaacaga ccgtcgatga tcttgtcgtt
    28981 gccgttggcg tcctgggtga tggcctggcc atggatgttg gtggctacgc cgcccatcat
    29041 gtagtggcag gtcggcacga ccggaaccgg agcagtcacc ggatcgacgt gagcgaaggt
    29101 cttggacagt tcgcagatgc ctggcaggcg gctgtgcaac acttcctcgc ccaggtgatc
    29161 gagcttcagc agcacgtgat cgccatccgg accacagccg ttgccggcga tgatttcctt
    29221 gaccatggag cgcgcaacca cgtcgcggcc ggccaggtcc ttggcgttcg gcgcataacg
    29281 ctccatgaag cgttcgccgt gcttgttgat caggtagcca ccctcgccgc ggcaaccttc
    29341 ggtcaccagc acgccggcac cggcgatgcc ggtcgggtgg aactgccaca tttcgatgtc
    29401 ctgcaccggc acgccggcac gcagggccat accaatgccg tcaccggtat tgatcagggc
    29461 gttggtggtg gacgcgtaga tgcgaccagc accgccggtg gccagcacgg tggccttggc
    29521 gcggatgtag gccgtctcgc cggtctcgat gcagatggcg atgatgccga cgatggcgcc
    29581 gtcctggttc ttcaccagat cgaccgcgaa ccactcgttg aggaaggtgg taccgttctt
    29641 caggttggcc tggtacaggg tgtgcagcag ggcgtgaccg gtacggtcgg ccgcggcaca
    29701 ggtacgagca gcctgggtcg gattatccgg acccttggac tggccgccga acggacgctg
    29761 gtagatgcga ccctgttcgg tacgggagaa cggcagcccc atgtgctcga gctcgaacac
    29821 ggcttccggg ccgacggagc acatgtattc gatagcgtcc tggtcaccga tgtagtcgga
    29881 acccttgacg gtatcgtaca tgtgccagcg ccaatcgtcg ttcgggtcgg ccgaagcgat
    29941 tgcgcaggtg atgccaccct gggcggatac ggtgtgcgaa cgggtcggga agaccttggt
    30001 caccacggca gtcttgtgtc ccgactggga cagctgcagc gcagcgcgca tgccggcacc
    30061 gccgccaccg atgatgatgg cgtcgaagga aagagtagga atgttagcca tggatcagat
    30121 accccaaaga atctgcacac cccagacaaa gaacgcgaac atggcgatgc cgcagactgc
    30181 ctggaacagg aaacgcacag cggtggccgc tttgcccagc gccatcggcg tcaggtagtc
    30241 agtggagata gtccacatac cgacccacgc atgcacgctc agggcaacga tgcccagcag
    30301 gctgaagatg cgcatccagt tctgggagaa caggccatgc cagtcggaga aagtcagact
    30361 ggggctggct acgagatatc ccaacagaaa gaggaaataa gccgcgagaa ccacagcgga
    30421 aacccgctgc gccatccagt cgtacagacc cgaacgggag aagttagtga cgttggttac
    30481 catacccaca cccctgcaag aacgatcagc accgcggcaa cggcgatgac aattttggag
    30541 cccagctttc cgccttccag cgtttcacca atgcccatat ccatgatcag atggcgaata
    30601 ccggctacca gatggtacag cagcgcggac aacaggcccc aaatcacgag cttggccaga
    30661 gggctggtca gacacgcctt cacctgctcg aagccttcct cggacgccag ggacaggtcg
    30721 agtgcgtaca gcagcacagc gatgcccaag aacaaaatga cgccggaaat gcggtgagcg
    30781 atggacgtgt aagcagtgac tgggagttgg atggtcctaa ggtctaggtt tacgggtcgt
    30841 ttgctattca cggcttcttt tcacactgag agccccaacc agcagggcaa gttgtcggga
    30901 agtgcacttg tcaggtaccc atcaccaggg agtgacaaca gccagaacca aggactggca
    30961 tagcctctgg cggtcggctg tgaagtatag acagttaggg tatcgatgac aacgcgcacc
    31021 cctagcctaa aggacggatt gtcgcagggg aaaaaagggc gtaaacaggc gctcttcatg
    31081 gccaaaagtg tcggcacaca gcccggacaa acatcctttt ggacaaattg acattcgatt
    31141 ttatcgctct atagtggcac cggccctgcc tggggggctg ctgtatagat gatgatttca
    31201 cgcataattt ttaggaggcc ggtatggctg acaagaaagc acagttgatc atcgagggcg
    31261 ctgcccccgt cgagctgccc atcctctctg gcaccatggg ccccgacgtg atcgacgtcc
    31321 ggagcctgac caaccagggc gtcttcacct atgatcctgg cttcatgtcc accgctgctt
    31381 gcgagtcgaa gatcaccttc atcgacggtg acaagggtgt tctgctgcac cgcggctacc
    31441 cgatcgagca actggccgag aagtccgact atctcgagac ctgctacctg ctgctgaacg
    31501 gcgaactgcc gaacgcagag cagaaggctc acttcaccgg cctggtgaag aaccacacca
    31561 tggttcacga gcagatgaag tcgttcctca acggcttccg tcgcgatgct cacccgatgg
    31621 ccgtgatgtg cggcatggtc ggcgccctgt ccgccttcta ccatgactcg ctggacaaca
    31681 acaatccgca gcaccgcgaa atctctgcga tccgcctgat cgccaagatg ccgaccattg
    31741 ccgccatggc gtacaagtac tccgccggcc agccgatcat gtatccgcgc aatgacatgg
    31801 gctacgcgga aaacttcctg cacatgatgt tcagcacccc gtgcgaggac aagccggtca
    31861 acccggtact ggcccgcgcc atggatcgca tcttcgttct gcacgccgac catgagcaga
    31921 atgcatccac ctccaccgtg cgcctggcag gttccaccgg tgccaacccg ttcgcctgta
    31981 tcgccgcggg catcgctgcc ctgtgggggc cggctcacgg cggcgccaac gaagccgtgc
    32041 tgaccatgct cgacgagatc ggcagcgtcg agaacatcga gaagttcatc gccaaggcca
    32101 aggacaagaa cgacccgttc aagctgatgg gcttcggtca ccgcgtgtac aagaacttcg
    32161 acccgcgcgc caaggtcatg aaaaagacct gcgacgaagt actgcaggaa ctgaacatca
    32221 acgatccgaa actcgaactg gccatgaagc tggaagagat tgcgcgcaac gatccttact
    32281 tcaaggagcg caacctgtac ccgaacgtcg acttctactc gggcatcatc ctgaaggcca
    32341 tcggcattcc gaccagcatg ttcaccgtga tcttcgccct ggcacgtacc gccggctgga
    32401 tctcccagtg gaaggaaatg ctctccactc cttataagat cggccgcccg cgtcagatct
    32461 acaccggcta tccaacccgc gactacgcgc cgctgaacag ccgcaagtaa gaactgcgca
    32521 aacacaaaag ggctgcttcg gcagcccttt tgcttttcag cgccatcaac cccgcactgc
    32581 ctctcccctc ctcctgcacg cagacgcacg ggtcatagcc ttgctcgcgc gccgtcacgc
    32641 cgacacgcgt ctatagccac ctgccaccta cactccgcgc atgcactgtc attctgcggc
    32701 gatcatggag tcggtctccg tgatgaccgg caagcagccc taccaagaca tgatcaagtt
    32761 cttgggcaag ctgttcggca tcaacttcgc cctcggcgac atcttcggcg cgccgctggc
    32821 catcgaggga ctgatggcgt tcctccccgc gccgaccttc atcggcctgt ccttcttcgg
    32881 ctggaaccgc ctgatcctcc acccgttggg cggctggctg ctgatggggc tgggctacct
    32941 catccgctgg cgcgccagcc acgactgatc gcgccgatca ggcggtcagt tgctccagct
    33001 cggtgagcag acggacagcc tgggcatccg tttcgccaca cacctcgtga tcggcggaga
    33061 atcgcgagca gaccgctggc cgctcgggct ggccgaacag ccggcagaga ttgtgctcat
    33121 ccagctgtac gcagcgcaca ccggccggct tgccctgcgg catgccggga atcggcgagg
    33181 aaatcgacgg ggcgacgcag caggccccac aaccggctcg acattgcatg acgactacca
    33241 tccgaaaagt ccaggacaca ggagcggcgc gtgacatcga ccgcaaggcg cgcgagcata
    33301 gccgccgctc cggcactcgc caagagcaac ccatcgtcat cccgtggagt cagccccatg
    33361 ccggtttgga tgcagaccgc cgccctgctc agcctgtcca acatcttcat gaccttcgcc
    33421 tggtacggcc acctgaagac cctcaacggc aagccgtgga tcatcgccgc gctgatcagc
    33481 tggggaatcg ccctgttcga gtatctgatc atggtgccgg ccaaccgcat cggctacagc
    33541 gagatgtcgg tcggccagct gaagatcatg caggaagtca tcaccctgag catcttcgtg
    33601 ccgttcagcg ttctgtacat gcagcagccg ctgaaactgg actacttgtg ggccggcctg
    33661 tgcctgctcg gcgcggtgta cttcatcttc cgcagcgcct aaacgcgccg gcccggctgg
    33721 atcaggagcg cacctgcggc ttcaggcgcg tgatcaggct cgaagtatcc cagcgcccac
    33781 cgccggcagc ctgcacgtcg gcatagaact gatcgaccag tgcggtgacc ggcagttgcg
    33841 cgccgttgct gcgcgcctcg tcgagcagga tgcccagatc cttgcgcatc cagtccaccg
    33901 cgaaaccgaa gtcgaagcgc ccctccagca tgctggcatg acggttctcc agctgccagg
    33961 actgcgccgc gcccttgctg atcacctcca tcgccgcatt gccgtcgaga ccggcacact
    34021 gagcgaaatg cagcgcctcg gacaggccct gcagcaggcc gccgatacag atctggttga
    34081 ccatcttggt cagctggccg ctgccggcgt gccccatcag gcgcaccatc cgcgcatagc
    34141 cctggatcac cggctcggcg cgggcgaaca ccgccggctc gccaccgacc atcaccgtca
    34201 gcacgccgtt ctgcgccccg gcctggccgc cggaaaccgg cgcatcgaga aactccagct
    34261 cgcgttcggc cgccagggct gccagctcgc gggccacgct ggccgacgcc gtggtgtgat
    34321 cgaccagcac actgcccggc gccataccgg caaaggcgcc gtccgcgcct agcacgaccg
    34381 aacgcaggtc atcgtcgttg cccacgcagc tcatcacgaa ctcggcaccc gccaccgcct
    34441 cgcggggggt cggcgcactg cgcccgccat gctcggccac ccagcgctgc gccgtctccg
    34501 cggtgcggtt gtacacgcac agctcatgcc cgtgccgcgc cagatgaccg gccatcggat
    34561 agcccattac ccccaacccc agaaacgcga ccttcgccat cattcacctc cagtgagcga
    34621 gttcccgagc ctagcacagg ggtttgccgc cacagtaggc tgcggccata ctcgaggcgc
    34681 acagaccaag gagcggagca tgaaacactg gctggtaatc gacctggaag ccaccacgga
    34741 caagaacggc tggccggtcg aggaaatgga gatcatcgag atcggcgcct cgatcgtcga
    34801 cgaacagggg cgcgagcagg ctcacttcca gagtttcgtc cagccgaccc gccacccgca
    34861 gctcaccgcc ttctgccgcg aactgaccca catccgccag tccgacgtgg acagcgcgcc
    34921 gaccctcagg caactgcacg cgaccttcga gccctggctg caacagcatc tgggcaacct
    34981 gcagggctgg ctcagctggg gcgactacga ccgccagcag ttcgagatcg aatggcgcaa
    35041 gcagcagatc gccagctgcc tggccagcct gccgcacttc aacctgaaga agctgttcca
    35101 ccgccagttc aagggcgtgg tcggccgcaa gcgggtcggc ctgaacaggg ctctggagat
    35161 gaccggcctg acgtttctcg gtacccagca ccgcggcctc gatgatgcgc gcaacatcgc
    35221 ccgcctgctg cccgccacgc tgccggtact ggccgccgcg cgcctggaag cccagcaagg
    35281 gcaacaggcg cggcagtagc agctgccttc atctcttcgg cctgcgcgcc cggcttgtgc
    35341 acctgcggct cttcagcacc tgaagggccg gcaggcgccg ccaggacctc cccgcctgca
    35401 tgaacaaaat tcatgcaaac cccactgcga cgccaatccc ctgcagcttt gctatcattc
    35461 ccgcctttac gatccaccct ctccaggtag tccccatgtc cgatcgcgcc gcccgcctcc
    35521 aggccctcca gaacgccctc aaggaacgca tcctgatcct cgacggcggc atgggcacca
    35581 tgatccagag ctacaagctg caggaagagg actaccgcgg cgaacgcttt gctgactggc
    35641 cgagcgacgt caagggcaac aacgacctgc tgctactgtc gcgcccggac gtgatccagg
    35701 ccatcgagaa ggcctacttg gacgccggcg ccgacatcct cgagaccaac accttcaacg
    35761 ccacccgcgt gtcccaggcc gactacggca tggaagcgct ggcctacgag ctgaacgtgg
    35821 aaggcgcgcg cctggcccgt gaagtggccg acgccaagac cgccgagacc ccggacaagc
    35881 cgcgcttcgt cgccggcgtg gtcggcccga ccagccgcac ctgctcgatc tccccggacg
    35941 tgaacaaccc cggctaccgc aacgtcacct tcgacgagct ggtggagaac tacaccgaat
    36001 ccacccgcgg cctgatcgcc ggcggcgccg acctgatcct gatcgagacc atcttcgaca
    36061 cccttaacgc caaggcggcg atcttcgccg tgcagcaggt gttcgaggaa gaaggcgtcg
    36121 aactgccgat catgatttcc ggcaccatca ccgacgcctc cggccgcacc ctgtccgggc
    36181 agaccaccga ggcgttctgg aactcggtgc gccacgccaa cccgatctcc gtaggcctca
    36241 actgcgccct cggcgccaag gacctgcgcc cgtacctgga agagctggcc accaaggccg
    36301 acacccacgt gtccgcccac cccaacgccg gcctgcccaa cgccttcggc gaatacgacg
    36361 agaccccggc acagatggcc gcggtggtcg aggagttcgc cgccagtggc ttcctcaaca
    36421 tcatcggcgg ctgctgcggc accaccccgc cgcacatcca ggcaatcgcc gaggcagtgg
    36481 ccaagtaccc gccgcgcgtc attccggaaa ttcccaaggc ctgccgcctg tccggcctcg
    36541 agccgttcac catcgaccgc aacagtctgt ttgttaacgt cggcgagcgc accaacatca
    36601 ccggctcggc ccgcttcgcc cgcctgatcc gcgaggagaa ctacaccgag gccctcgaag
    36661 tcgccctgca gcaggtggaa gccggcgccc aggtgatcga catcaacatg gacgagggca
    36721 tgctcgactc gaaggcggcg atggtcacct tcctcaacct gatcgccggc gagccggaca
    36781 tctcgcgcgt gccgatcatg atcgactcct ccaagtggga agtgatcgag gccggcctca
    36841 agtgcatcca gggcaagggc atcgtcaact cgatctccat gaaggaaggc gtcgaggcct
    36901 tcaagcacca cgcccgcctg tgcaagcgct acggcgccgc cgtggtggtg atggccttcg
    36961 acgaggccgg ccaggccgac accgccgcgc gcaagcgcga gatctgccag cgcagctacg
    37021 acatcctggt caacgaagtc ggcttcccgc cggaagacat catcttcgac ccgaacatct
    37081 tcgccgtggc caccggcatc gacgagcaca acaactacgc ggtcgacttc atcgaggcct
    37141 gcgcctacat ccgcgacaac ctgccctacg ccctgagctc gggcggcgtg tccaacgtgt
    37201 cgttctcgtt ccgcggcaac aacccggtgc gcgaggcgat ccactcggta ttcctctacc
    37261 acgccatcca gaacggcctg accatgggca tcgtcaacgc cggccagctg gagatctacg
    37321 acgagatccc caagcagctg cgtgatgcgg tcgaggacgt ggtactcaac cgccacgctg
    37381 gcagcaccga agcgctgctg gccatcgccg accagtacaa gggcgacggc tcggtcaagg
    37441 aagccgagac cgaggaatgg cgcagcctgc cggtcggcaa gcgcctggag cacgcgctgg
    37501 tcaagggcat caccgccttc atcgtcgagg acaccgagga gttccgccag cagtgcgcgc
    37561 ggcccatcga ggtcatcgaa ggcccgctga tgagcggcat gaacgtggtc ggcgacctgt
    37621 tcggcgccgg caagatgttc ctgccccagg tagtcaagtc cgcgcgggtg atgaagcagg
    37681 cggtggcgca cctgatcccc ttcatcgagg cggaaaaggg cgacaagccg gaagccaagg
    37741 gcaagatcct catggccacc gtgaagggcg acgtccacga catcggcaag aacatcgtcg
    37801 gcgtggtgct cggctgcaac ggctacgaca tcgtcgatct cggcgtgatg gtgccggcgg
    37861 agaagatcct ccaggtcgcc aaggaagaga agtgcgacat catcggcctg tccggcctga
    37921 tcaccccgtc gctcgacgag atggtccacg tcgccaaaga aatgcagcgc cagggcttca
    37981 ccctgccgct gatgatcggc ggcgccacca cctccaaggc gcacaccgcg gtgaagatcg
    38041 agccgcaata cagcaacgac gcggtggtct acgtcaccga cgcctcgcgc gccgtgggcg
    38101 tggccaccac cctgctgtcc aaggaactca agcccgacta cgtggccaag ctgaagaccg
    38161 aatacgccga agtgcgcgaa cgcaccgccg cacgcagctc gcgcaccgag cgcctgagct
    38221 acgacaagg
//
LOCUS       DATCUN010000066         2761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_152894, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000066 DATCUN010000000
VERSION     DATCUN010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152894"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            963..>2761
                     /locus_tag="VIO83_02640"
     CDS             963..>2761
                     /locus_tag="VIO83_02640"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEY8330211.1"
                     /translation="MTERVQAGGLQVAKVLFDFVNNEAIPGTGVSAEQFWQGADALIH
                     DLAPKNKALLAKRDALQAQIDAWHQARAGQAHDAVAYKAFLQEIGYLLPEPADFAVTT
                     ENVDDEIARMAGPQLVVPVMNARFALNAANARWGSLYDALYGTDAISEEDGAEKGKGY
                     NPVRGAKVIAFARDFLNEAAPLEGASHVDSTGYRIDGGKLVVSLANGSTAGLKNPAQL
                     IGFQGEASAPQAVLLKNNGIHFEIQIDPTSMIGKTDAAGVKDVLMEAALTTIMDCEDS
                     VAAVDADDKVIAYKNWLGLMKGDLAEEVAKGGTTFTRTMNPDRVYTAANGGELTLHGR
                     SLLFVRNVGHLMTNDAILDKDGNEVPEGIIDGLVTSLIALHNLKGNTSRKNTRTGSVY
                     IVKPKMHGPEEAAFTNELFGRVEDVLGMTRNTLKVGIMDEERRTTVNLKACIKAASER
                     VVFINTGFLDRTGDELHTSMEAGAFIRKGDIKNAAWIKAYENWNVDIGLKAGLSGRAQ
                     IGKGMWAMPDLMAGMLEQKIAHPLAGANTAWVPSPTAAALHALHYHKVDVFARQQELA
                     KRERASLDDILTIPLAPSISWSAEEIQNELDNNA"
BASE COUNT          584 a          995 c          790 g          392 t
ORIGIN      
        1 actggcgccg gccaacctgc tggctttcgc ctgcatctgg gcagccctgc tggtgtacag
       61 cctggacatc tggcgagtcc tccgccgccg ctgatcattt tctgcacgat gccctgcagg
      121 tcacgccagc cgtggcctgc agggcattgc cccaaggtta tccacacact gatccccgcc
      181 agttgtgcac aagcaggctc caggccgctg tgcaaaaaac acccagaaca ccgccagagc
      241 ccatacaggg cggggcctgg cgatccatcc cgagcttatc cacaggctgg tcccccgcaa
      301 cggtggacat cttcacgcca aattccgcca aggacgctgg tcaaaatccg accaccgcgc
      361 cacaagcccc gccaggcatg gcctgcagcg atgtacccgc agcttgtcca caccctggtg
      421 cacgccctgt gtggacaacc ctgcacttgt gcacaaggac atatccccag cgccaacgac
      481 ttttccccaa gcgggggggc aagcacccgt acaaaaaacc accaatcgcc gccagggccc
      541 gccaggcctg gcctgcagac agggctccag agcttatcca caggcgcata caccctaccg
      601 gtgcataacc cgcaaagacc tgatcaaaaa acacccacaa gcctgtaacc cacgccgcac
      661 tagggctgca gcactgtacc caaggcttgt ccacaggcgg gtccacggca aacggggaca
      721 actgtggaaa accgtcatgc aggcgccaca cacccgctcc ccgcccgctg tggacaaccc
      781 gtccgccgcg gctatcctca agcagcgccc cctacttggg ccgcactttt ccacgacttt
      841 tgtcgaaagc cgtcctgccc ttcatacatt tctcatacgg catcatgtag tatccttcga
      901 tcagtactac acgaacggcg ggccgcagtc cgatgcggct cccaagcaga gtgaggcaag
      961 caatgactga acgcgttcaa gccggtggcc tgcaggtcgc caaagtcctg ttcgacttcg
     1021 tcaacaacga agccattccc ggcaccggcg tcagcgccga gcagttctgg cagggtgccg
     1081 acgccctgat tcatgacctg gctccgaaga acaaggccct gctggccaag cgcgatgccc
     1141 tgcaggcgca gatcgacgcc tggcaccagg cgcgtgccgg ccaggcccac gatgccgttg
     1201 cctacaaggc cttcctgcag gagatcggct acctgctgcc ggagccggcg gacttcgccg
     1261 tcaccaccga aaacgtcgac gacgagatcg cccgcatggc cggcccgcag ctggtagtgc
     1321 cggtgatgaa cgcgcgcttc gccctcaacg ccgccaacgc ccgctggggt tcgctgtacg
     1381 acgccctgta cggcaccgac gcgatcagcg aagaagacgg cgccgagaaa ggcaaaggct
     1441 acaacccggt gcgcggcgcc aaggtcatcg ccttcgcccg cgacttcctc aatgaagccg
     1501 caccgctgga aggcgcttcc cacgtcgact ccaccggcta ccgcatcgac ggcggcaagc
     1561 tggtcgtcag cctggccaac ggcagcaccg ccggcctgaa gaacccggcc cagctgatcg
     1621 gcttccaggg cgaagccagc gcgccgcagg ccgtgctgct gaagaacaac ggcatccact
     1681 tcgagatcca gatcgatccg accagcatga tcggcaagac cgacgcggcc ggcgtgaagg
     1741 acgtgctgat ggaagccgcg ctgaccacca tcatggactg cgaggactcg gtcgccgcgg
     1801 tcgatgccga cgacaaggtc atcgcctaca agaactggct gggcctgatg aagggcgacc
     1861 tggccgagga agtggccaag ggtggcacca ccttcacccg caccatgaac ccggaccgcg
     1921 tctacaccgc ggcgaacggc ggcgaactga ccctgcacgg ccgttccctg ctgttcgtgc
     1981 gcaacgtcgg tcacctgatg accaacgacg ccatcctcga caaggacggc aacgaggtgc
     2041 cggaaggcat catcgacggc ctggtcacca gcctgatcgc cctgcacaac ctcaagggca
     2101 acaccagccg caagaacacc cgcaccggct cggtgtacat cgtcaagccg aagatgcatg
     2161 gcccggaaga agccgccttc accaacgagc tgttcggccg cgtcgaggac gtcctcggca
     2221 tgacccgcaa caccctcaag gtcggcatca tggacgagga gcgccgcacc accgtcaacc
     2281 tcaaggcgtg catcaaggcc gccagcgagc gcgtggtgtt catcaacacc ggcttcctcg
     2341 accgcaccgg tgacgagctg cacacctcca tggaagccgg cgcgttcatc cgcaagggcg
     2401 acatcaagaa cgccgcctgg atcaaggcct acgagaactg gaacgtcgac atcggcctga
     2461 aggccggcct gtccggccgt gcgcagatcg gcaagggcat gtgggccatg cccgacctga
     2521 tggccggcat gcttgagcag aagatcgccc acccgctggc cggcgccaac accgcctggg
     2581 taccgtcgcc gaccgccgcc gcgctgcacg ccctgcacta ccacaaggtc gacgtgttcg
     2641 cccgtcagca ggagctggcc aagcgcgagc gcgcctcgct ggacgacatc ctgaccatcc
     2701 cgctggcgcc gagcatcagc tggagcgccg aagagatcca gaacgaactg gacaacaacg
     2761 c
//
LOCUS       DATCUN010000067         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_246858, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000067 DATCUN010000000
VERSION     DATCUN010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246858"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VIO83_02645"
     CDS             <1..708
                     /locus_tag="VIO83_02645"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HEY8330212.1"
                     /translation="LNFGCPAKTVNKSRGGAVLLKEPELLHAIAREVSATLPAGVPLT
                     AKMRLGYDSPDGALDCARALVDGGATMLVVHARTKLDGYKPPAHWEWVARVQEAVKVP
                     VYANGEIWTVDDWRRCREVSGADNIMLGRGLVSRPDLARQIAAASAGREPEPLSWGEL
                     QPLLQLFWRKARQKLAPRYAPGRLKQWLAMLTRSYPEAQALFDSIRRDNDCARLDALF
                     GLRAGELSQSAVEAIPG"
     gene            complement(823..1710)
                     /locus_tag="VIO83_02650"
     CDS             complement(823..1710)
                     /locus_tag="VIO83_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8330213.1"
                     /translation="MDLASLDTFIAIAETGSFSAAGERLHLSQPAVSKRIASLEQQLE
                     VRLFDRIGREVSLTEAGRALLPRAWQILNVLDDTRRALSNLNGDVSGRLTLATSHHIG
                     LHRLPPLLRAFTRAHPQVALDIRFLDSEVAYEEVLHGRTELAVITLAPHTAAPVRAVP
                     VWDDPLDFVAAPEHPLAHGGPVTLAEVAQHAAVFPGENTFTHHIVQRLFEEAGLKPNV
                     SMSTNYLETIKMLVSIGLAWSVLPRTMLDDQVARLALQGIQLTRQLGYIVHTERTLSN
                     AARAFMQLLDRAGAEAQAS"
     gene            1834..>2355
                     /locus_tag="VIO83_02655"
     CDS             1834..>2355
                     /locus_tag="VIO83_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY8330214.1"
                     /translation="MAGKTLYDKLWDMHLVKQRDDGSALIYIDRQILHEVTSPQAFEG
                     LRLAGRKPWRVDANIATPDHNVPTTKAERQGGLESIHDEVSRLQVQTLDENCDDFGIL
                     EFKMNDRRQGIVHVVGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLA
                     TQCLVAKKMKNMQV"
BASE COUNT          394 a          764 c          833 g          364 t
ORIGIN      
        1 ctcaatttcg gctgcccggc caagacggtg aacaagtcgc gtggcggtgc ggtgctgctc
       61 aaggagcccg agctgctgca tgccatcgcc cgcgaagtca gtgccacgct gccggccggg
      121 gtgccgctga cggcgaagat gcgcctaggc tacgacagcc ccgacggcgc gttggactgc
      181 gcacgggccc tggtcgacgg cggtgcgacc atgctggtgg tgcatgcaag gaccaagctc
      241 gacggctaca agccgccggc gcactgggag tgggtggcgc gggtgcagga ggcggtcaag
      301 gtgccggtct atgccaatgg cgaaatctgg acggtggacg actggcggcg ctgtcgcgag
      361 gtcagcgggg cggacaacat catgctcggc cgcggactgg tgtcgcgccc ggacctggcc
      421 cggcagatcg ccgccgccag cgccggccgc gaacccgaac cgctgagctg gggcgagctg
      481 cagccgctgc tgcagctgtt ctggcgcaag gcccgccaga agctcgcccc gcgctacgcg
      541 ccagggcgac tcaagcagtg gctggccatg ctgacgcgca gctatcccga ggcgcaggcg
      601 ctgttcgaca gcattcgccg cgacaacgac tgcgcgcggc tggatgcatt gttcgggttg
      661 cgcgccgggg agctgtccca gtctgcggtc gaggccattc cggggtaggg tgggttaggc
      721 cgcaggccgt aacccaccga ccgggccgca ggctcggcgc ttttgcaatg cgtgatcttg
      781 cggtcgcgat ggtgggttac gccgctgcgc ggctaaccca ccctacgaag cttgcgcctc
      841 ggccccggct cggtccagca actgcatgaa cgcccgcgcc gcgttggaca gggtgcgctc
      901 ggtgtgcacg atgtagccga gctggcgggt cagctggata ccctgcagcg ccaggcgcgc
      961 cacctggtcg tcgagcatgg tgcgcggcag cacgctccag gccaggccga tcgacaccag
     1021 catcttgatc gtctccaggt agttggtgct catgctcacg ttgggcttca ggccggcctc
     1081 ctcgaacagg cgctggacga tgtggtgggt gaaggtgttc tcgccgggga acaccgccgc
     1141 atgctgcgcc acctcggcca gggtcaccgg cccgccgtgg gccagcgggt gttcgggggc
     1201 ggcgacgaag tctagcgggt catcccatac cggcaccgcg cgtaccggcg cggcggtatg
     1261 cggggcgagg gtgatcaccg ccagctcggt gcgcccgtgc agcacctcct cgtaggccac
     1321 ctccgaatcg agaaagcgga tgtccagcgc cacctgcgga tgggcgcggg tgaaggcgcg
     1381 caacagcggc ggcaggcggt gcaggccgat gtgatgactg gtcgccaggg tcaggcggcc
     1441 actgacatcg ccgttgagat tgctcagcgc gcggcgggtg tcgtccagca cattgagaat
     1501 ctgccaggcg cgcggcagca gggcgcggcc ggcctcggtc aggctgacct cgcggccgat
     1561 gcggtcgaac aggcggactt ccagttgctg ttcgaggctg gcgatgcgct tgctcaccgc
     1621 cggctggctg aggtgcaggc gttcgccggc ggcggaaaag ctgccggttt cggcgatggc
     1681 gatgaaggtg tcgaggctgg ccagatccat gctcggattc cattaggtta tccaaagcat
     1741 gaaaaatatg aatttgtgtt atccgaagca agtccctagc atcgaccgca tacgccctgt
     1801 gggcattcag caacaaccga tgaggagatc cccgtggccg gcaagacgct ttacgacaag
     1861 ctgtgggaca tgcacctggt caagcagcgc gacgatgggt cggcgctgat ctacatcgac
     1921 cgccagatcc tccacgaagt gacctccccg caggccttcg aaggcctgcg cctggccggc
     1981 cgcaagccgt ggcgcgtgga tgccaacatc gccaccccgg accacaacgt gccgaccacc
     2041 aaggccgagc gccagggcgg tctcgagtcg atccacgacg aagtgtcgcg cctgcaggtg
     2101 cagaccctcg acgagaactg cgacgacttc ggcatcctcg aattcaagat gaacgaccgt
     2161 cgccagggca tcgtccacgt ggtcggcccg gagcagggcg ccaccctgcc ggggatgacc
     2221 gtggtctgcg gcgactcgca cacctccacc cacggcgcct tcggcgcgct ggcccacggc
     2281 atcggcacct ccgaggtcga gcacgtgctg gcgacccagt gcctggtggc caagaagatg
     2341 aagaacatgc aggtg
//
LOCUS       DATCUN010000068         4980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_40543, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000068 DATCUN010000000
VERSION     DATCUN010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40543"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="VIO83_02660"
     CDS             <1..1235
                     /locus_tag="VIO83_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HEY8330215.1"
                     /translation="PLKHFLIEALIPYEADEVTRLIIDTHDAAAFAPVSHLTVGGLRD
                     WLLGDAADEACLRALAPGLTPEMAAAVSKIMRVQDLILVAQKIRVVTRFRNTQGLRGR
                     MSTRLQPNHPTDDPAGIAASTLDGLLYGNGDAMIGINPATDSMGSICTLVEMLDAVIQ
                     RYEIPTQSCVLTHVTSSIAAIERGAPLDLVFQSIAGTEAANASFGINLKVLQEGYEAG
                     MSLKRGTLGNNLMYFETGQGSALSANAHHGVDQQTCETRAYAVARHFKPFLVNTVVGF
                     IGPEYLYNGKQIIRAGLEDHFCAKLLGVPMGCDICYTNHAEADQDDMDMLLTLLGAAG
                     INFIMGIPGSDDVMLNYQTTSFHDALYARQLLGLRAAPEFEDWLARMGILHQQGGRVR
                     LGDELPPAFRQALAELGP"
     gene            1368..2177
                     /gene="eutC"
                     /locus_tag="VIO83_02665"
     CDS             1368..2177
                     /gene="eutC"
                     /locus_tag="VIO83_02665"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HEY8330216.1"
                     /translation="MPDKSPATENPWQQLRGLTPARIALGRAGTSLPTAAQLDFQFAH
                     AQARDAVHLPFDPALLAEGLRQRGLDSLALHSAARDRDTYLQRPDLGRRLHADCAAQL
                     REHAAVHGGGYDLAIVIADGLSALAVQRHSLPLLDRLLEQVRAEGWSLAPVSLVEQGR
                     VAVADEVGELLGARMTVILIGERPGLSSPDSLGLYFTWAPKVGLTDAYRNCISNVRLE
                     GLSYGMAAFRLLYLMREACRRQLSGVDLKDEAEVPSLDGGGKGNFLLEGGR"
     gene            complement(2261..4162)
                     /locus_tag="VIO83_02670"
     CDS             complement(2261..4162)
                     /locus_tag="VIO83_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEY8330217.1"
                     /translation="MSATPINSSHEQAHSSSALGLLVAATGVVYGDIGTSPLYTLKEV
                     FAGHYGVQVNHDGVLGILSLIFWSLLWVVSLKYVLFILRADNGGEGGVIALTALARRA
                     TAAHPRLSSGLVLLGLFGAALFYGDSMITPAISVLSAVEGLELAYEGVSHWVIPLSLA
                     VLIGLFLIQKHGTARIGILFGPVMVAWFVVLGALGVYGIQRNPEVLLALNPWWGLNFF
                     LAHPGIGVAILGAVVLALTGAEALYADMGHFGRKPIARAWFILVLPGLLLNYFGQGAL
                     LLDNPEAARNPFYLLAPEWALLPMVGLATLATIIASQAVISGAFSLTQQAIQLGYVPR
                     MQIQHTSSAEQGQIYIGTVNWALMVGVVLLVLGFETSGALAAAYGVAVTGTMLITTLL
                     VAAVMLLLWKIPRALAIPLLLGFLLVDSLFFAANIAKIFQGGAFPVVAGIALFTLMTT
                     WNRGKQILFARLDETALPLPVFIDSIRLQPPHRVEGTAVFLTARPDAVPHALLHNLLH
                     NQVLHEQVVLLTVVTEDSPRLPVERRFEVEHYGEGFFRIILHFGFMEEPDVPAALRLC
                     RLDGLDFSPMRTTYFLSRETVIPSKRIGMANWREGLFAFMQRNSNSSMRYFQLPVNRV
                     IELGTQVEI"
     gene            complement(4159..4869)
                     /locus_tag="VIO83_02675"
     CDS             complement(4159..4869)
                     /locus_tag="VIO83_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8330218.1"
                     /translation="MTERQQTVLVVDDEAQIRKFLRISLAAHGYRVLEAGSGREGLDL
                     AQQAKPDLLVLDLGLPDMDGKAVLGELRRGSSMPVLVLSVRAGDSEKVLCLDGGANDY
                     VTKPFSIPEFLARVRVLLRQPAASGVQEASVRCGPLQIDLASRRVSLDDREIGLTRKE
                     FAVLATLARQLGRVVTQQQLLTDIWGPTHVADSHYLRVVVGHLRQKLGDNPADPYFII
                     TEAGIGYRLRERPSGASQ"
BASE COUNT          818 a         1740 c         1673 g          749 t
ORIGIN      
        1 tgccgctcaa gcacttcctc atcgaggcgc tgattcccta cgaggccgac gaggtcaccc
       61 ggctgatcat cgacactcac gatgccgccg ccttcgcccc ggtcagccac ctgaccgtcg
      121 gcggtctgcg cgactggctg ctcggcgatg cggccgacga ggcctgcctg cgcgcgctgg
      181 cgccggggct gaccccggag atggccgccg cggtgtcgaa gatcatgcgc gtgcaggacc
      241 tgatcctggt ggcgcagaag atccgcgtgg tcactcgttt ccgcaacacc cagggcctgc
      301 gcgggcgcat gtcgacccgc ctgcagccca accacccgac cgacgatccg gccggcatcg
      361 ccgccagcac cctcgacggc ctgctctacg gcaacggcga cgcgatgatc ggcatcaacc
      421 cggccaccga cagcatgggc tcgatctgca ccctagtgga gatgctcgac gcggtgatcc
      481 agcgttacga gatccccacc cagtcctgcg tgctgaccca cgtcaccagc tcgatcgccg
      541 ccatcgagcg cggcgcgccg ctggacctag tgttccagtc gatcgccggc accgaggcgg
      601 ccaacgccag cttcggcatc aatctgaagg tgctgcagga gggctacgag gccgggatgt
      661 cgctcaagcg cggcaccctc ggcaacaacc tgatgtactt cgagaccggc cagggcagcg
      721 cgctgtcggc caacgcccac cacggcgtcg accagcagac ctgcgagacc cgcgcctacg
      781 cggtggcccg ccacttcaag ccgttcctgg tcaacaccgt ggtcggcttc atcggcccgg
      841 agtacctgta caacggcaag cagatcatcc gcgccggcct cgaggaccac ttctgcgcca
      901 agctgctcgg cgtgccgatg ggctgcgaca tctgttacac caaccatgcc gaggccgacc
      961 aggacgacat ggacatgctg ctgaccctgc tcggcgcggc ggggatcaac ttcatcatgg
     1021 gcatccccgg ctccgacgac gtgatgctca actaccagac cacctcgttc cacgacgccc
     1081 tgtacgcccg ccagctgctc ggcctgcgcg ccgcgccgga gttcgaggac tggctggcgc
     1141 gcatgggcat cctgcaccag cagggcggcc gcgtgcgcct gggcgacgag ctgccgccgg
     1201 cgttccgcca ggcgctggct gagctcggtc cataagcctg ggacgacgtg cccggtccac
     1261 gcctgtaggg gcgaattcat tcgccatggc gcctgccgca aggcgaatga attcgcccct
     1321 acaggtggac tacccaaccc aacccaaccg gcacccggag gtttccgatg cccgacaagt
     1381 cccccgccac cgagaacccc tggcagcaac tgcgcggcct gaccccggcg cgcatcgccc
     1441 tcggccgtgc cggcaccagc ctgcccaccg ccgcgcagct ggacttccag ttcgcccacg
     1501 cccaggcgcg cgatgccgtg cacctgccgt tcgatcccgc tctgcttgcc gaagggttgc
     1561 gccagcgcgg cctggatagc ctggcgctgc acagcgccgc cagggaccgc gacacctacc
     1621 tgcagcgccc cgatctcggc cgccgcctgc atgccgactg cgccgcgcaa ttgcgcgagc
     1681 acgccgctgt ccatggcggc ggctacgatc tggccatcgt catcgccgat ggcctgtccg
     1741 cgctggcggt gcaacggcac agcctgccgc tgctcgaccg cctgctcgaa caggttcgcg
     1801 ccgaaggctg gagcctggcg ccggtgagcc tggtcgagca gggccgggtg gcggtggccg
     1861 acgaggtggg cgagctgctt ggcgcgagga tgacggtgat cctgatcggc gagcgccccg
     1921 gcctcagctc gccggacagt ctcggcctgt acttcacctg ggcgccgaag gtcggcctga
     1981 ccgatgccta tcgcaactgc atctccaacg tgcgcctgga gggcctcagc tacggcatgg
     2041 ccgccttccg cctgctgtac ctgatgcgcg aagcctgtcg ccggcagttg tccggtgtgg
     2101 atctcaagga cgaggccgag gtgccgagcc tggacggcgg cggcaagggc aatttcctgc
     2161 tcgaaggcgg acgctgagag cctgtcccaa ggctctggct cgcgagatcg tgaacaatcg
     2221 ctcggtgtgg agtgaggggc gccggtcggc gccctctgct tcagatctcc acctgcgtcc
     2281 ccagctcgat cacccggttc accggcaact ggaagtagcg catgctgctg ttggagttgc
     2341 gctgcatgaa ggcgaacagc ccttcgcgcc agttggccat gccgatgcgt ttgctcggga
     2401 tcacggtttc ccggctgagg aagtaggtgg tgcgcatcgg gctgaagtcc agcccgtcga
     2461 gacggcacag gcgcagcgcg gcgggcacgt ccggctcctc catgaagccg aagtgcagga
     2521 tgatgcggaa gaaaccctcg ccgtagtgct ccacctcgaa gcgccgctcc acgggcagcc
     2581 gcgggctgtc ttcggtcacc acggtcagca gcaccacctg ctcgtgcagc acctggttgt
     2641 gcagcaggtt gtgcagcagc gcgtgcggta cggcgtccgg ccgcgcggtg aggaacaccg
     2701 cggtgccctc gacccgatgc ggcggctgca ggcggatgct gtcgatgaac accggcagtg
     2761 gtagggcggt ttcgtccagc cgtgcgaaca ggatctgctt gccacggttc caggtggtca
     2821 tcagggtgaa cagggcgatg ccggccacta ccgggaaggc gccgccctgg aaaatcttgg
     2881 cgatgttggc ggcaaagaac aggctgtcga ccagcaggaa gcccagcagc agcggaatcg
     2941 ccagagcgcg cgggatcttc cacagcagca gcatcaccgc ggccaccaat agcgtggtga
     3001 tcagcatggt gccggtcacc gccacgccgt aggccgcggc cagcgcaccg gaggtttcga
     3061 agccgagcac cagcaatacg acgccaacca tcagcgccca gttcaccgtg ccgatgtaga
     3121 tctgcccctg ctcggcgctg gaagtgtgct ggatctgcat gcgcggcaca tagccaagct
     3181 ggatggcctg ctgggtcagg gagaacgccc cggaaatcac cgcctgcgag gcgatgatgg
     3241 tggccagggt ggccaggccg accatcggca gcagcgccca ctccggcgcc agcaggtaga
     3301 aggggttgcg cgccgcctcg gggttgtcca gcagcagcgc gccctggccg aagtagttga
     3361 gcagcaggcc cggcagcacc aggatgaacc aggcgcgggc gatcggcttg cggccgaaat
     3421 ggcccatgtc ggcgtacaac gcctcggcgc cggtcagcgc cagcaccacc gcaccgagaa
     3481 tcgccacgcc gatgcccggg tgggcgagga agaagttcag cccccaccag gggttgagcg
     3541 ccagcagcac ttccgggttg cgctggatgc cgtacacgcc gagcgcgccc agcaccacga
     3601 accaggccac catgaccggg ccgaacagga tgccgatgcg tgcggtgccg tgcttctgga
     3661 tcaggaacag gccgatcagt accgccagcg acagggggat cacccagtgg ctgacgcctt
     3721 cgtaggcgag ttccaggcct tcgacggcgg acagcacgga aatcgccggg gtgatcatgc
     3781 tgtcgccgta gaacagcgcg gcgccgaaca ggccgagcag caccagtccc gaactcagcc
     3841 gcggatgggc cgcggtcgcg cggcgggcca gggcggtcag cgctatcacc ccgccttcgc
     3901 ccccgttgtc ggcgcgcagg atgaacagca cgtacttgag cgacaccacc cacagcagcg
     3961 accagaagat cagcgagagg atgccgagca cgccgtcgtg gttgacctgc acgccgtaat
     4021 gcccggcgaa cacctccttg agggtgtaca gcgggctggt gccgatatcg ccgtagacca
     4081 cgccggtggc agcgaccagc aggcccagcg cggaggagga gtgcgcctgc tcgtggctgg
     4141 agtttatggg ggttgcgctc attggcttgc tcctgagggc cgctcgcgca gccggtagcc
     4201 gatgccggct tcggtgatga tgaaatacgg atcggctggg ttgtcgccga gtttctggcg
     4261 cagatggccg accaccacgc gcaggtagtg gctgtccgcg acgtgggtcg gtccccagat
     4321 atcggtcagc agttgctgct gggtgaccac gcggcccagc tggcgggcca gcgtggccag
     4381 caccgcgaac tccttgcggg tcaggccgat ttcccggtca tccaggctca cccggcggct
     4441 ggcgaggtcg atctgcagcg gcccgcagcg cacgctcgct tcctgcacac cggaagctgc
     4501 cggctggcgc agcagcacgc gcacgcgggc gaggaactcg gggatgctga acggcttggt
     4561 cacgtagtcg ttggcgccgc cgtccaggca cagcaccttc tcgctgtcgc cggcgcgcac
     4621 cgagagcacc agcaccggca tcgacgaccc gcggcgcagt tcgccgagca cggccttgcc
     4681 gtccatgtcc ggcaggccga ggtcgagcac cagcaggtcg ggcttggctt gctgcgccag
     4741 gtccaggccc tcgcggccgc tgccggcttc cagtacccgg tagccgtggg cggcgaggct
     4801 gatgcgcagg aacttgcgga tctgcgcctc gtcgtcgacg accagcaccg tctgttgcct
     4861 ctcggtcatt gcgcctctcc ctggtcttcc cggtcggcgt cgtcgagccg cggctgcgcc
     4921 agcagcggca ggtgcagggt cagcgtggtg ccgcgcccgt ccaggccgtc gccgacgctg
//
LOCUS       DATCUN010000069         5574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_247188, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000069 DATCUN010000000
VERSION     DATCUN010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247188"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VIO83_02680"
     CDS             complement(<1..139)
                     /locus_tag="VIO83_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017001525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY8330219.1"
                     /translation="MDNAFTTLLGLRHPLIQAPMAGGATTPELVAAVSNAGALGSFAA
                     AT"
     gene            complement(191..673)
                     /locus_tag="VIO83_02685"
     CDS             complement(191..673)
                     /locus_tag="VIO83_02685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8330220.1"
                     /translation="MGKHDIAAVLAIQEESYASELLESTEIIRQRLLAFPDLAWVAED
                     AHGVCAYLFGYRSHAGKVTPLDGAFAEPDAPDCLYLHDLAVARRAAGRGIGPALVQRN
                     LEHGLASRLKYSALVSVQDSAAFWSRLGYAAEAALDSAQQRNLASYGVPAVYMVKPLH
                     "
     gene            complement(820..1437)
                     /locus_tag="VIO83_02690"
     CDS             complement(820..1437)
                     /locus_tag="VIO83_02690"
                     /inference="COORDINATES: protein motif:HMM:NF017816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HEY8330221.1"
                     /translation="MRNLHCTSAKRAGVAHLHCATSFLALALLAPVAGAEVSATLAYT
                     DYLIQGDQRGSLARLLEDTSPIHADGQTYHAFTTWQVSWRYSWISEASGACRIAQVAT
                     AVHSTIKLPRLEGGSPSLRERFERYLGALHEHELGHHQIGHQAALEVDRQLQGMPPMA
                     DCQRLNTAANKLAQRTVNQFKEEERTYDLITVHGRLQGARLDYDY"
     gene            1444..1641
                     /locus_tag="VIO83_02695"
     CDS             1444..1641
                     /locus_tag="VIO83_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330222.1"
                     /translation="MSRAVSGVAAVPVGGRKIPSARNVTGKRNALSDQWILVLANLKL
                     AVELKVISGGIYDALRFETVD"
     gene            2234..2572
                     /locus_tag="VIO83_02700"
     CDS             2234..2572
                     /locus_tag="VIO83_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEY8330223.1"
                     /translation="MATISPNDISTTVLRRMKEGGFDFARIHPIEFYAIFPDEERARQ
                     AARSFRGESLKAEVTQRDGDWHLQVSKVMYATHAGIDDFEHDLEVLVGPLGGVLDGWG
                     VTQEVGEARP"
     gene            2846..4285
                     /locus_tag="VIO83_02705"
     CDS             2846..4285
                     /locus_tag="VIO83_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY8330224.1"
                     /translation="MVRSTYDEMYNAQGDCRAHYQAFARWLAATPAEQLEQRRREADL
                     LFHRAGITFTLYGDEQGTERLIPFDTIPRSIPAREWQQIEQGCIQRVRALNMFLTDLY
                     HDQRIIKAGIIPAEQVLANEGYQLAMQGLDLPHNLYAHIAGIDLVRDGDGTYYVLEDN
                     LRTPSGVSYMLENRKMMMRLFPELFAGQRIAPVNHYPNLLLDTLKSSSLVDQPSVVVL
                     TPGRYNSAYFEHAFLAREMGVELVEGADLFVRDERVFMRTTAGPKAVDVIYRRLDDAF
                     LDPLAFNPDSMLGVPGLLAAYRRGNVVLANAIGTGVADDKSIYPYVPEMIRFYLDEEP
                     ILANVPTWQCRKPAELSHVLANLEQLVVKETQGSGGYGMLVGPAASQAEIETFRARLK
                     ANPAAYIAQPTLSLSTCPTFVDSGIAPRHIDLRPFVLSGQQVRLVPGGLTRVALREGS
                     LVVNSSQGGGTKDTWVVEEPTAAPESREV"
     gene            4298..5242
                     /locus_tag="VIO83_02710"
     CDS             4298..5242
                     /locus_tag="VIO83_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEY8330225.1"
                     /translation="MLSRTAADLYWMSRYLERAENMARMLDVSHSLSLMPQGGRGDGL
                     EELAMPLLITGTLDAYLERHGELDAERLLHFFALDADNPASLYSCLRAARVNAHAVRG
                     RITADMWENINATWLEMRSIAAQAPADFNASGFCEWVKERSHLFRGATEGTIMRANAY
                     HFIRLGTFIERADNTLRLLDARHQLMGDELPSDSSARAYFQWSALLRALTAYEAHAAL
                     YRGSPKVAQVAELLLLRGDVPRSLRSCIAALSQILAEIPGTNGRAAQRMAAELDARLR
                     FTGIEEVLDEGLKAWIHDYILLVRQLGNAIHSAYLEAV"
     gene            5239..>5574
                     /locus_tag="VIO83_02715"
     CDS             5239..>5574
                     /locus_tag="VIO83_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HEY8330226.1"
                     /translation="MKLAIHHDTTYRYADRVRASIQYLRLTPQDSARQRVLDWQLELP
                     QAAQAMRDAHGNILHVLTLDEPHQAVVIGAHGHVEIDESCESESEAGSPLPYLRSTAL
                     TAADAALREF"
BASE COUNT          932 a         1795 c         1868 g          979 t
ORIGIN      
        1 gggtggcggc ggcgaaggag cccagcgcgc cggcattgct gaccgcggcg accagctccg
       61 gcgtggtcgc cccgccggcc atgggcgcct ggatcagcgg gtggcgcagg ccgaggaggg
      121 tggtgaaggc gttgtcgatc aggggcatga gggggggctt ccagcaacga aacgagcccc
      181 cagtctgccc tcaatgcagc ggcttgacca tgtacaccgc cggcacgccg taggaggcca
      241 ggttgcgctg ctgggcgctg tccagcgccg cctcggcggc gtagcccagt cggctccaga
      301 acgccgccga gtcctgtacc gacaccagcg ccgagtactt gagccggctg gccaggccgt
      361 gttccaggtt gcgctgcacc agtgccgggc cgatgccgcg cccggcggcg cgccgggcca
      421 cggccagatc gtgcagatac aggcagtcgg gcgcgtccgg ctcggcgaag gcgccgtcca
      481 gcggcgtcac cttgccggcg tgcgagcggt aaccgaacag gtaggcgcac acgccgtggg
      541 catcttcggc cacccaggca aggtcaggga aggccagcag ccgctggcgg atgatctcgg
      601 tgctttccag cagttcgctg gcgtaggact cttcctggat ggcgagcacg gcggcaatgt
      661 cgtgtttgcc catcaggcgc aggtgatagc tcatgggggc gttcgaaggg gatcgctcga
      721 aggaatgaga ggcggattgg ccgggcagca ttattccaga tcggagcgaa cccgtggccg
      781 cccgccgacc gcctgcgcat gggcggccgg ccgcgcagct cagtagtcgt agtccaggcg
      841 cgcgccctgc aggcggccat gcaccgtgat caggtcgtag gtgcgctcct cttccttgaa
      901 ctggttgacg gtccgttgcg cgagcttgtt ggccgccgtg ttcagccgct ggcaatcggc
      961 catcggcggc atgccctgca actgccggtc gacctccagg gcggcctggt ggccgatctg
     1021 gtggtggccc agttcgtgct catgcagcgc gcccagatag cgctcgaagc gttcgcgcag
     1081 gctggggctg ccgccttcga gcctgggcag cttgatggtg ctgtgcacgg ccgtggcgac
     1141 ctgggcgatc cggcaggcgc cgctggcttc gctgatccag ctgtagcgcc agctgacctg
     1201 ccaggtggtg aaggcatggt aggtctgccc gtcggcgtgg atcggcgaag tgtcttcgag
     1261 gagtctcgcc agcgatccgc gctggtcacc ctggatcagg tagtcggtgt aggccagcgt
     1321 tgcgctgacc tcggcgccgg caacgggcgc cagcagagca agggccagga aggaagttgc
     1381 acaatgcagg tgcgcaactc ccgcccgctt tgcagaagtg caatgcaggt ttcgcatgga
     1441 gtcatgtctc gtgccgtttc aggagttgcc gcagtccctg ttggcgggcg caagattcct
     1501 tctgcaagaa acgtcactgg aaaacgcaat gcattgtccg atcagtggat tcttgtactt
     1561 gcaaacttga aactagcagt cgagttgaag gttatttctg gcgggatata tgacgcgttg
     1621 cgatttgaaa cggtggatta atttggattt attgggtcgc catgtttcgt cgagaatctg
     1681 gcggcaatgg tttgttttac cgcgggtctc gcctagcccg tcgctggcgc aggccttgcc
     1741 tgcgggtgcc ggggcggctg tgcgcgtcgg ggcatggcga gcgccgaagt tgccgatcct
     1801 gggccgctga caaggctgca tccgctggcg ccggaacaac ctggcagtcc aggtgacggg
     1861 agtcggcgac gagttgtcgg gaaattctgc gccagtgaat agttggtagg gctattggtt
     1921 tttgacgctg ttggttggcg gttatctggc gtgctgctgg ttgttaatat tgaccgttgg
     1981 tcgggttgtc cggttttgca tgaattcgtc actgcgtgga tgaaagtttc attggtccgc
     2041 ctataaataa aacccacgtc tcgatcccgt tcgcgcgggg gaactcgcga agccagccga
     2101 tggttgaggg gaggggacgg cttgcgcggc gcagcggccg gcatcagtgg ctatacagcg
     2161 ccaggcgttt ggggctagtc tgggcgtgct ggtgaaaatt caaatgatgg ctgagaaaag
     2221 aggagtgcgt gcaatggcga cgatttctcc aaacgatatc agcaccaccg ttttgcgccg
     2281 gatgaaagag ggcggtttcg attttgcccg gattcatccc atcgagtttt atgccatttt
     2341 cccggacgaa gaacgggcac ggcaggcggc aaggagtttt cgcggcgagt ccttgaaagc
     2401 cgaggtcacc cagcgcgatg gcgactggca tctgcaagtc agcaaggtga tgtacgccac
     2461 ccacgccgga atcgacgact tcgagcatga cctggaggtc ctggtcggcc cgttgggcgg
     2521 ggtgctggat ggctggggtg tcacccagga agtaggcgaa gcgcgtccct gatccactgc
     2581 tgttgctggc aaagaagccc accgctcacc cggtgggctt cttttttgtg cagccggcgc
     2641 gcgccgtggc aggcggatag cgccatggca ctgccaggct gcctcgtttt gaggcgcgct
     2701 gcgcaggaag gtgcgaagtc ggtgcaccgt cttggcgcga caaatgctgc agatcgctcc
     2761 aatgccttga tttacagggc gttacaaaag tggcgcgggc cttgcaatgt ctctggcaag
     2821 aggcacagac agggagcccg cgggcatggt tcgcagcact tacgatgaaa tgtacaacgc
     2881 ccagggcgat tgccgggcac attatcaggc ctttgcccgc tggctggcag ccacgccggc
     2941 cgagcagctg gagcagcgcc gacgcgaggc cgatctgctg ttccaccgcg cggggatcac
     3001 cttcaccctg tacggcgacg agcagggcac cgagcgcctg attcccttcg acaccatccc
     3061 gcgcagcatc ccggcgcgcg agtggcagca gatcgagcag ggctgcatcc agcgcgtgcg
     3121 cgcgctgaac atgttcctca ccgacctcta tcacgaccag cgcatcatca aggccggcat
     3181 catcccggcc gagcaggtgc tggccaacga gggctaccag ctggccatgc agggcctcga
     3241 tctgccgcac aacctctatg cgcatatcgc cgggatcgat ctggtgcgcg acggcgacgg
     3301 cacctactac gtgctggaag acaacctgcg cacccccagc ggggtttcct acatgctgga
     3361 aaaccgcaag atgatgatgc gcctgttccc cgagctgttc gccggccagc gcatcgcccc
     3421 ggtcaaccac tacccgaacc tgctgctgga caccctgaag agctccagcc tggtggacca
     3481 gcccagcgtg gtggtgctga ctcccggccg ctacaacagc gcctacttcg agcacgcctt
     3541 cctcgcccgc gagatgggcg tcgagctggt ggagggcgcc gacctgttcg tgcgcgacga
     3601 gcgcgtgttc atgcgcacca ccgccgggcc gaaggcggtg gatgtgatct accggcgcct
     3661 cgacgacgcc ttcctcgatc cgctggcctt caacccggac tccatgctcg gcgtgcccgg
     3721 cctgctggcg gcctaccgcc gcggcaacgt ggtgctggcc aacgccatcg gcaccggggt
     3781 ggccgacgac aagtcgatct atccctacgt gccggagatg atccgcttct acctggacga
     3841 ggagccgatc ctcgccaacg tgccgacctg gcagtgccgc aagccggccg agctgtccca
     3901 cgtgctggcc aacctcgagc agctggtggt caaggaaacc cagggctccg gcggctacgg
     3961 catgctggtc ggcccggccg ccagccaggc cgagatcgag accttccgcg cccgcctcaa
     4021 ggccaacccg gcggcctaca tcgcccagcc gaccctcagc ctgtcgacct gcccgacctt
     4081 cgtcgacagc ggcatcgccc cgcggcacat cgacctgcgt ccgttcgtgc tgtccggcca
     4141 gcaggtgcgc ctggtccccg gcggcctgac ccgcgtggcc ctgcgcgagg gctcgctggt
     4201 ggtcaactcg tcgcagggcg gcggcaccaa ggacacctgg gtggtcgagg aaccgaccgc
     4261 cgcccccgaa tcccgcgaag tctgagcgag aaccgtcatg ctgagtcgta ctgctgccga
     4321 tctgtactgg atgtcgcgct acctggagcg cgcggaaaac atggcgcgga tgctggatgt
     4381 cagccattcg ctgtcgctga tgccccaggg cggacgcggc gatggcctcg aggaactggc
     4441 catgccgctg ctgatcaccg gcaccctgga tgcctacctg gagcgccacg gcgagctgga
     4501 cgccgagcgc ctgctgcact tcttcgccct cgatgcggac aatccggcga gcctgtacag
     4561 ctgcctgcgc gcggcgcggg tcaatgccca tgcggtgcgt gggcggatca ccgccgacat
     4621 gtgggagaac atcaacgcca cttggctgga gatgcgcagc atcgccgccc aggccccggc
     4681 ggacttcaat gccagcggct tctgcgagtg ggtcaaggag cgctcgcacc tgttccgcgg
     4741 tgccaccgaa ggcaccatca tgcgcgccaa tgcctaccac ttcatccgtc tcggcacctt
     4801 catcgagcgc gccgacaaca ccctgcgcct gctcgatgcc cgtcaccagc tgatgggcga
     4861 cgaactgccc agcgactcat cggcgcgcgc ctatttccag tggagcgcgc tgctgcgcgc
     4921 gctgacggcc tacgaggcgc atgccgcgct gtatcgcggc tcgccgaagg tcgcccaggt
     4981 cgccgaactg ctgctgctgc gcggcgacgt gccgcgttcg ctgcgctcgt gcatcgccgc
     5041 gctgagccag atcctcgccg agattcccgg taccaatggc cgtgccgccc agcgcatggc
     5101 cgctgaactg gatgcgcggc tgcgcttcac cggcatcgag gaagtgctcg acgaggggct
     5161 caaggcctgg attcacgact acatcctgct ggttcgccag ctgggcaatg ccatccacag
     5221 tgcctatctg gaggctgtat gaaacttgcc atccatcacg acaccaccta tcgctacgcc
     5281 gaccgcgtgc gcgccagcat ccagtacctg cgcctgaccc cgcaggacag cgcgcgccag
     5341 cgcgtgctcg actggcagct ggagctgccg caggcggcgc aagccatgcg cgatgctcac
     5401 ggcaacatcc tccacgtgct gaccctcgac gagccgcacc aggccgtggt catcggcgcg
     5461 cacggccatg tggagatcga cgagagctgc gaaagcgaga gcgaagcggg ctcgccgctg
     5521 ccatacctgc gcagcaccgc gctgaccgcg gccgacgcgg cgctgcgcga gttc
//
LOCUS       DATCUN010000070        11067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_40807, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000070 DATCUN010000000
VERSION     DATCUN010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11067
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40807"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1066)
                     /locus_tag="VIO83_02720"
     CDS             complement(107..1066)
                     /locus_tag="VIO83_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8330227.1"
                     /translation="MNKLFLDRPAQRLARLLFSSRGLVWLAYLGVVLIWATTPLAIQW
                     GTLDTGFAFAAVTRMFIALVCCLLVLGLCGIRLPRHRRALLSYLVAGGGLFVAMLAVY
                     WAARRIDSGLMSVIFGLSPLVTSLLAAFWLEERLLNPTRLLGMVLGVAGLGVVFIDDG
                     LQLAEGATAGIAVLLFAVLVNAATLVGLKRIADDSAPLATTAGSLLVAAPLFLLAWWL
                     DGAALPAQLSWRGGLSILYLALFGSVLGFALYYYIVRRLDSAQVALILVITPLLALLV
                     GQRFNGEQLPAQVWLGAVVIGLGLLLHQRGNRAVAAPQGLSDR"
     gene            complement(1152..2114)
                     /locus_tag="VIO83_02725"
     CDS             complement(1152..2114)
                     /locus_tag="VIO83_02725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007940326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEY8330228.1"
                     /translation="MDSTYLEVRIARKAAEALDIVTLELTSTSGEALPAFSAGSHIDV
                     EIRPGLVRQYSLCNDPTEQHRYIIGVLRDPISRGGSIAVHDALEEGQTIRISPPRNHF
                     PLVPAGRHVLFAGGIGVTPILCMAERLASSGADFVMHYSARSAERAAFVERIGSSAYA
                     GKVQFHFDDGAAEQKLDLPAVLAAEPADSHLYVCGPGGFIDFVANTARAQGWSDERIH
                     FEYFAAKAVDTSADQSFEVQVASTGKVYVIAEDQSIVSVLEADGVEISVSCQQGICGA
                     CITRVLDGQADHRDQVLTDKERDEENWFTPCCSRSRSPRLVLDI"
     gene            complement(2137..3771)
                     /locus_tag="VIO83_02730"
     CDS             complement(2137..3771)
                     /locus_tag="VIO83_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8330229.1"
                     /translation="MRKDISGRQVRLDEAILARYAGCSEWQNQTIAERAAAMAARDPQ
                     QVTHVFEGVPCRVGELLGDARLLAGALQRRGLVAGDVISFQLPNWRETAVIDLAASLL
                     GLVVAPIVPIYRDAEVAFMLADSAIKAAFFPGSYRNFDYAAMMQRLAPDLPQLRLLCP
                     VRCETAGADSYEAMLAEGKGPASLPEVDANAVKLILYTSGTTGRPKAVLHSHNTGPTR
                     LKRAFEHWCGSGAETLLMASPVTHVTGLSGLELPFYRNARTVFMDRWNAAEALEIIER
                     EQVTVSLGATPFLHELIHQAEQHGRRLPSLKAFACGGAEVPPALIYKAREVFDNCRAF
                     RVFGSSEAPLVTLGFVGDGQLEQAATTDGEICDYEVRLLDEEGQPVAAGQEGEICVRG
                     AAMFLGYADPEQTAASFDADGFFATGDLGRITADNAIVITGRKKDLINRGGEKISAKE
                     VEDILHQHPAIDEAAVVSMPHQRLGETLCAYVILKPGQDLTLETLLGSIGQAGVARQK
                     LPERLQVVADFPRTASGKIRKDQLRADIRQRLAAQP"
     gene            complement(3810..4904)
                     /locus_tag="VIO83_02735"
     CDS             complement(3810..4904)
                     /locus_tag="VIO83_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8330230.1"
                     /translation="MTTDIETISMLRDSLERYTAEQYDFLKRWRVLEQPAGYSEAAWQ
                     AYAEFGWLALRLPEEEGGLDGDAVAQGAVMEVVGKRLLMEPLLASALVGTGLLVKLGT
                     ASQQAALLPALAEGRLKLALAHEEPLDGQTCRVEAGRLSGAKQGVLHGDVANRLLVSA
                     RDEQGEQQVYLVDAAGAGVERRSYRLVDGRGAANLRFAGAPAERLGDAGGVAAAEALA
                     EACDEASVALCAEAFGAATCLVDITNEYLKVRRQFGNSLASNQALQHRMADLYMLKEE
                     IRALTRAAQQAMLLPAEERARLVSGAKAYISHAARQIANEAIQMHGGVGVTEELEVSH
                     YFRRLMVNSALFGSRDWHFSRFVESTLKSA"
     gene            complement(4901..6094)
                     /locus_tag="VIO83_02740"
     CDS             complement(4901..6094)
                     /locus_tag="VIO83_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8330231.1"
                     /translation="MNKTLRDRAQRLREEVREFCKHEMPAALRDKSHRHQVLDKDDYL
                     QWMRLLDAKGWVVGHWPQAYGGRDWSSLERFVFEDELARHGCPWIIPFGVKYVGPVIY
                     TYGSEEQKRRFLPGIVKTEEFWAQGYSEPGAGSDLAGLRTQAVRDGDHYVVNGQKVWT
                     TYAQWADWLFCLVRTSNDGKPQSGISFLLIDMRSPGVTVKPIHTMDGYHHVNEVWLEN
                     VRVPAVNLVGEEGAGWTYAKFLLKNERTAGAIVGLAWHVLERLKKLARDTQVEGGRLI
                     DQPLMRNRIGKFELRFMALQEVAYQAVESMVEGTDNGGEASLIKVRGTELYQEVAEAL
                     VEALGSAGIAYDVAALHSAGLPPLGDDDVGGILKEHFYNRAATVFGGSSEIQRNIVAK
                     AVLGL"
     gene            complement(6167..7561)
                     /locus_tag="VIO83_02745"
     CDS             complement(6167..7561)
                     /locus_tag="VIO83_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY8330232.1"
                     /translation="MNNPKHAKFTVLPPEEVGDPNLFQQLLKLETVPVPEHLQPNHPG
                     FSDEDLSVERYSSRAFHELEAEKLWTKAWQMVCREEDIQKPGDHVVYDIVNTSIIVMR
                     GQDHEIRGFHNSCLHRGRALRMSSGAVNQLRCPYHGFAWDLQGELKTMPTTWDFKHLK
                     PCDQKLPQVKVETWAGFVFINPDLDAPSLREHLDVLPEHFKDYNFEHSVSIAHVQRRM
                     ACNWKVAQEAFMESMHTRATHPQIMTFTGDIDSQYDMYGDNISRSVTPMAVTSSNLKD
                     VEEALILQDILDESGRMAESDSSKHVLPEGMTTRQYIGELNRKIFAEAAGVDLSHATL
                     AELQDAILYSIFPNTQVWAGYFGNIVYRAIPDGDNHEACLFDVWLLGRYPAGTQCPPG
                     AKLNRLPDDLEFSAAAELGALGPVFDQDMSNLDAMTKGLKASKKGAVSLASYQESRIR
                     HHHLTLDKYLGLAK"
     gene            complement(7605..8753)
                     /locus_tag="VIO83_02750"
     CDS             complement(7605..8753)
                     /locus_tag="VIO83_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY8330233.1"
                     /translation="MEDIAIIGVGLHPFGRTDGLSGQAQGAYAVREALRDAGVEWHQV
                     GVAYGGSQDGGNADALANHLGLTGIPFVNVANGCATGGSALASVIAALRSGMAEIGVA
                     VGFDKHPRGAFNPKPADWGLEDWYGETGLMLTTQFFALKTQRYLHEHGIAEEALVHVA
                     QRAFRNGALTPHAWRQRELSFAEIAESMPVNLPLRKYMFCNPAEGGAALVLCRASEAH
                     KYSRNPVLVRAAVMRTRHHGSFEVFSPGLAAEQSPAPTVTASRAAFELAGVGPSDIDV
                     IQLQDTDSGSEIIHLAENGFCEHGEQGHLLCRGETDIGGRLPVNTDGGCLANGEPVGA
                     SGLRQVIEVCTQLRQQAGARQVPGRPRLGYTHVYGAPGVSAVTILEAP"
     gene            complement(8753..9202)
                     /locus_tag="VIO83_02755"
     CDS             complement(8753..9202)
                     /locus_tag="VIO83_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY8330234.1"
                     /translation="MSDLMQTNLAGGHLLRAGAQGPILLGSRCRQCGECYFPIADNCT
                     RCSATEFERCEIGRQGRLWSWTIQGFLPKAPYNSGESEVDFQPYGVGYVEMPCGVKVE
                     SRLTVADPQQLHIGMPMELTLVRYGRNAVGEPLHTFAFAPLAAEGDE"
     gene            complement(9297..9920)
                     /locus_tag="VIO83_02760"
     CDS             complement(9297..9920)
                     /locus_tag="VIO83_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8330235.1"
                     /translation="MRTQILDATMQCLIGIGYTQTTTEKIAKQAGVSRGAMTHHFKSR
                     AEVFAAAAEYITDIRVREYDAAIREVSRRAGGQPTQESMLETLKVLQEYFMQPSFLAF
                     HELVRGARTDAELHAVMAPLEQSLDERISQSIEAHFPIWAEAGMRETGECLRDLIMHA
                     LRGVALDPSSYLHGDRLHRLHELLAKTAMAEYMEALKRKVQLQAQAN"
     gene            complement(10113..10904)
                     /locus_tag="VIO83_02765"
     CDS             complement(10113..10904)
                     /locus_tag="VIO83_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8330236.1"
                     /translation="MNNNNSANVQDQFSLEGRVALVTGAGTGLGAHFARVLARAGAKV
                     ACVARRLDRVEAVAADIRAQGGEAFACVLDVTDGASIKQACDSIERELGLIDVLVNNA
                     GLSDPAPFEEMSRTQWTSLLDANLSGPFFVAQEMAKRLIVAGKPGSIINIASILGHLA
                     KDKFVNYGTTKAGLIHMTQYMALDLIPYSIRVNALAPGYFPSEMTNPFYESEVGKREI
                     ASLPPKRLGRHEELDGPLLLLASNASSYMSGSVVTVDAGHSIRLS"
     gene            complement(10925..>11067)
                     /locus_tag="VIO83_02770"
     CDS             complement(10925..>11067)
                     /locus_tag="VIO83_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015096483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEY8330237.1"
                     /translation="LVDGQVVLKHLLPLSLSYDHRVINGADAARFCRFVAEQLADPTL
                     LV"
BASE COUNT         1830 a         3697 c         3656 g         1884 t
ORIGIN      
        1 ttcgccggcc agctggaggc ggaaaaggcg gccttcagcg cctgcctgca gaccgaggat
       61 ttccgcgagg ccgtggccgc cttcctggaa aagcgccccg ccaccttcag cggtcgctga
      121 gcccctgcgg cgcggcaacc gcccggttgc cgcgctgatg caacagcaag cccagaccga
      181 tcaccaccgc gcccagccac acctgcgccg gcagctgctc gccgttgaag cgctgaccga
      241 ccagcagcgc cagcagcggc gtgatcacca ggatcagcgc cacctgcgcc gagtccagac
      301 gccgcacgat gtagtagtac agggcgaagc ccagcaccga gccgaacagc gccagataga
      361 ggatcgacag cccaccgcgc cagctcagct gcgccggcag cgccgcgcca tccagccacc
      421 aggccagcag gaacaacggc gccgccacca gcagggagcc ggcggtggtg gccagcggcg
      481 cgctgtcgtc ggcaatgcgc ttgagaccga ccagggtcgc ggcgttgacc agcaccgcga
      541 acagcaacac ggcgatgccc gccgtcgccc cctcggcgag ctgcaggcca tcgtcgatga
      601 agacgacgcc gagaccggcc actcccagca ccatgcccag caggcgtgtc gggttgagca
      661 ggcgctcctc cagccagaag gccgccagca ggctggtgac cagcggcgaa agcccgaaga
      721 tcaccgacat cagccccgaa tcgatgcgcc gcgccgccca atagaccgcg agcatggcga
      781 cgaacagccc gccgccggcc accagatagc tgagcaacgc gcgccgatgc cgcggcaggc
      841 ggatgccaca caggcccagg accagcaggc agcaaaccag ggcgatgaac atgcgggtca
      901 ccgcggcgaa ggcgaagccg gtatccagcg tgccccactg gatggccagc ggcgtggtcg
      961 cccagatcag cacgaccccc agataggcca gccagaccag cccccgacta ctgaacagca
     1021 gacgtgccaa ccgctgagcg ggccgatcca gaaacagctt gttcatcgca gaggaccttt
     1081 gccggcatga aaaaaaaggc acgccaggga tacggcgtgc cgaagggaca tccccacccc
     1141 agggacaccg gtcagatatc gagcaccagc cgcgggctcc gcgagcgcga gcagcacggc
     1201 gtgaaccagt tttcctcgtc gcgctccttg tcggtcagca cctggtcgcg gtggtccgcc
     1261 tggccgtcca gcacccgggt gatgcaggcg ccgcagatgc cctgctggca ggacacgctg
     1321 atctccacgc cgtcggcctc gagcaccgag acaatcgact ggtcctcggc gatcacatag
     1381 accttgccgg tgctggccac ctgcacctcg aagctctggt ccgccgaggt atccaccgcc
     1441 ttggcggcga aatactcgaa atggatgcgc tcgtcgctcc aaccctgcgc cctggcggtg
     1501 ttggcgacga agtcgatgaa gccgcccggg ccgcacacgt acaggtggct gtccgccggc
     1561 tcggccgcca gcacggcggg gaggtcgagc ttctgctcgg cggcgccgtc gtcgaagtgg
     1621 aactgcacct tgccggcata ggcggagctg ccgatgcgct cgacgaaggc cgcgcgctcg
     1681 gccgagcggg ccgagtagtg catgacgaag tcggcgccgc tgctggccag acgctcggcc
     1741 atgcacagga tcggggtgac gccgatgcct ccggcaaaca gcacatggcg cccggccggc
     1801 accagcggga agtggttgcg cggcgggctg atgcggatgg tctgcccttc ctcaagggcg
     1861 tcgtgcacgg cgatcgagcc gccgcgcgaa atcgggtcgc gcagcacacc gatgatgtag
     1921 cgatgctgct cggtcgggtc attgcacagc gagtactggc gcaccagacc cgggcgaatt
     1981 tccacgtcga tgtgcgaacc cgcactgaaa gccggcaggg cctctccgct ggtcgaggtc
     2041 agctcgagcg tcacgatatc cagcgcttcg gccgccttgc gggcgatgcg cacttcaaga
     2101 taggtagagt ccatactggc catctccgaa ttgttattag ggttgcgccg ccaggcgctg
     2161 gcggatgtcg gcgcgcagct ggtccttgcg gatcttgccc gaggcggtgc gcggaaaatc
     2221 agccaccacc tgcaggcgct cggggagctt ctgccgggcc acgccggcct ggccgatgct
     2281 gcccagcagc gtctccaggg tcagatcttg gccgggcttg agtatcacat aggcgcacag
     2341 ggtttcgccg aggcgctggt gcggcatgga caccaccgcc gcctcgtcga tggccgggtg
     2401 ctgatggagg atgtcctcga cctccttggc gctgatcttc tcgccgccgc ggttgatcag
     2461 gtccttcttg cgcccggtga tgacgatggc gttgtcggcg gtgatccgcc ccaggtcgcc
     2521 ggtggcgaag aagccgtcgg catcgaagct ggcggcggtc tgctccggat cggcgtagcc
     2581 gaggaacatg gcggcgccgc gcacgcagat ctcgccttcc tggcccgcag cgacgggctg
     2641 gccctcctcg tcgagcaggc gcacctcgta gtcgcagatc tcgccgtcgg tagtggccgc
     2701 ctgctccagc tggccgtcgc cgacgaagcc cagggtcacc agcggcgcct cgctggagcc
     2761 gaacacgcgg aaggcgcggc agttgtcgaa cacttcgcgg gccttgtaga tcagcgccgg
     2821 cggtacttcg gcacccccgc aggcaaaggc cttgaggctc ggcaaacggc gtccgtgctg
     2881 ctcggcctgg tggatcagct cgtgcaggaa cggcgtcgcc ccgaggctga cggtgacctg
     2941 ctcgcgctcg atgatctcca gcgcctcggc ggcgttccag cgatccatga acactgtgcg
     3001 cgcgttccgg tagaacggta gctcaaggcc gctgaggccg gtgacgtggg tcaccggcga
     3061 ggccatcagc agggtttccg cgccgctgcc gcaccagtgc tcgaaggcgc gcttgaggcg
     3121 ggtcggcccg gtgttgtgcg aatgcagcac ggccttgggc cgtccggtgg tgcccgaggt
     3181 gtagaggatc agcttgaccg cgttggcatc cacttccggc agcgaagccg gacccttgcc
     3241 ctcggcgagc atcgcctcgt agctgtcggc accagccgtc tcgcagcgca ccgggcagag
     3301 caggcgcagc tgtggcagat ccggcgccag gcgctgcatc atcgcggcgt agtcgaagtt
     3361 gcgatagctg cccggaaaga acgccgcctt gatcgccgaa tcggcgagca tgaaggcgac
     3421 ctcggcatcc cggtagatcg gcacgatcgg cgccaccacc aggcccagca ggctggcggc
     3481 caggtcgatg accgcggtct cgcgccagtt gggcagctgg aagctgatca cgtcgccggc
     3541 caccaggcca cggcgctgca gggcgccggc cagcaggcgg gcatcgccga gcagctcgcc
     3601 gacccggcag ggcacgccct cgaagacatg ggtgacctgc tgcgggtcgc gtgcggccat
     3661 ggccgcggcc cgctcggcga tggtctggtt ctgccactcg ctgcagccgg catagcgggc
     3721 caggatcgcc tcgtccaggc gcacctgccg gccactgata tctttgcgca tgcttacatc
     3781 ctcttgttgg ctacttatgg atgcgtcttt caagcggact tcagcgtcga ctcgacgaag
     3841 cggctgaaat gccagtcacg gctgccgaac agcgccgagt tgaccatcag gcggcggaaa
     3901 taatggctga cctccagctc ttcggtgacg ccgacgccac cgtgcatctg gatcgcctcg
     3961 ttggcgatct ggcgcgcggc atggctgatg taggccttgg cgccgctgac cagccgcgcc
     4021 cgctcctcgg ccggcagcag catcgcctgc tgggcggcac gggtcagcgc acggatctcc
     4081 tccttgagca tgtacaggtc ggccatgcgg tgctgcagcg cctggttgct ggccagcgaa
     4141 ttgccgaact gcctgcgcac cttgaggtac tcgttggtga tgtcgaccag gcaggtcgcc
     4201 gcgccgaagg cttcggcgca cagcgccacg ctggcctcgt cgcaggcttc ggccagcgcc
     4261 tcggctgccg ctacgccgcc ggcgtcaccc aagcgctcgg ccggcgcacc ggcaaatcgc
     4321 aggttggccg cgccgcgacc atcgaccagg cggtagcttc ggcgctcgac gcctgcaccg
     4381 gcagcatcga ccagatacac ctgctgctcg ccctgctcgt cgcgggcgct caccagcagt
     4441 cggttggcca catcgccgtg cagcacgccc tgcttggccc ccgacaggcg cccggcctcg
     4501 acccggcaag tctggccgtc gagcggctcc tcgtgggcca gcgccagctt caggcggcct
     4561 tcggccagcg ccggcagcag cgcggcctgc tggctggcgg tgcccagctt gaccaacagc
     4621 ccggtgccga ccagcgcact ggccagcagc ggctccatca gcaggcgctt gccgaccact
     4681 tccatgaccg cgccctgcgc cacggcatcg ccgtccaggc cgccctcctc ctcgggcagg
     4741 cgcagcgcca gccagccgaa ctcggcatag gcctgccagg ccgcctcgct gtaaccggcg
     4801 ggctgctcga gcacgcgcca gcgcttgagg aagtcgtact gctcggcggt atagcgttcc
     4861 aggctgtcac gcagcatgga gatggtttca atgtccgtag tcatagtccg agcaccgcct
     4921 tggccacgat attgcgctgg atctccgacg agccgccgaa gaccgttgcc gcgcgattgt
     4981 agaaatgttc cttgagaatg ccgccgacat cgtcgtcgcc cagcggcggc aagccggcgc
     5041 tgtgcagcgc cgcaacgtcg taggcgatcc ccgccgaacc caatgcctcg accagggcct
     5101 ctgccacttc ctggtacagc tcggtgccgc ggaccttgat cagcgaggcc tcgccgccgt
     5161 tgtcggtgcc ctcgaccatc gactcgaccg cctggtaggc gacttcctgc agcgccatga
     5221 agcgcagctc gaacttgccg atgcggttgc gcatcagcgg ctggtcgatc aggcggccac
     5281 cctcgacctg ggtgtcgcgt gccagcttct tcaggcgttc cagcacgtgc caggccaggc
     5341 cgacgatggc gccggcggtg cgctcgttct tcagcaggaa tttggcgtag gtccagcccg
     5401 cgccctcctc gccgaccagg ttgaccgccg gcacgcgcac attctccagc cacacctcgt
     5461 tgacgtggtg gtagccgtcc atggtgtgga tcggcttgac cgtcacgccg gggctgcgca
     5521 tgtcgatcaa caggaaggag atgccgctct gcggcttgcc gtcgttgctg gtgcgcacca
     5581 gacagaacag ccagtcggcc cactgcgcgt aggtggtcca gaccttctgg ccgttgacca
     5641 cgtagtggtc gccgtcgcgt accgcctggg tgcgcagacc ggccaggtcg gaaccggcgc
     5701 ccggctcgga gtagccctgg gcccagaact cctcggtctt gacgatgccg ggcaggaagc
     5761 gccgcttctg ctcctcgctg ccgtaggtgt agatcaccgg gccgacgtac ttgacgccga
     5821 aggggatgat ccacgggcag ccatggcggg ccagctcgtc ctcgaacacg aagcgctcca
     5881 gcgacgacca gtcgcgtccg ccgtaggcct gcggccagtg accgacgacc cagcccttgg
     5941 cgtcgagcag gcgcatccac tgcaggtagt cgtccttgtc gaggacctgg tgacgatggg
     6001 atttgtcgcg cagggctgcc ggcatctcgt gcttgcagaa ctcgcggacc tcttctcgca
     6061 ggcgttgcgc ccgatcgcgt agcgtcttgt tcatctcgct tcccttgttc tatgtccgcg
     6121 ccgctgtggg tggcgcgccg cagcggccac cccgggcaac gggcgtctac ttcgccaggc
     6181 ctaggtactt gtcgagggtc aggtggtgat ggcgaatgcg gctttcctgg tagctggcca
     6241 ggctgacggc gcccttcttc gaggccttga ggcccttggt catggcgtcg aggttgctca
     6301 tgtcttggtc gaagaccggg cccagcgcac ccagctcggc cgcggcggag aactccaggt
     6361 catcgggcag gcggttgagc ttggcgccgg gcgggcactg tgtgccggcc gggtagcgac
     6421 cgagcagcca gacatcgaac aggcaggcct cgtggttgtc gccgtcggga atggcgcggt
     6481 agacgatgtt gccgaagtag ccggcccaca cctgggtgtt ggggaagatc gaatagagga
     6541 tggcgtcctg cagctcggcc aacgtggcat gggacaggtc gaccccagcg gcttcggcga
     6601 agatcttgcg gttcagctcg ccgatgtact ggcgggtggt catgccctcg ggcagcacgt
     6661 gcttgctgct gtccgactcg gccatgcgcc cggactcgtc gaggatgtcc tgcaggatca
     6721 gggcttcctc gacatccttg aggttggagc tggtcaccgc catcggcgtg acgctgcggc
     6781 tgatgttgtc gccgtacatg tcgtactggc tgtcgatatc gccggtgaag gtcatgatct
     6841 gcggatgggt ggcccgggtg tgcatggact ccatgaaggc ttcctgggcg accttccagt
     6901 tgcacgccat gcgccgctgc acgtgggcga tggacaccga gtgctcgaag ttgtagtcct
     6961 tgaagtgctc cggcagcacg tcgaggtgtt cgcgcagcga gggcgcatcc aggtccgggt
     7021 tgatgaacac gaagccggcc caggtttcca ccttcacctg cggcagcttc tgatcgcacg
     7081 gcttgaggtg cttgaagtcc caggtggtgg gcatggtctt cagctcgccc tgcaggtccc
     7141 aggcgaagcc gtggtacggg cagcgcaact ggttgaccgc gccgctgctc atgcgcaggg
     7201 cacggccgcg gtgcaggcag gagttgtgga agccgcggat ctcgtggtcc tggccacgca
     7261 tgacgatgat cgaggtgttg acgatgtcgt acaccacatg gtcgcccggc ttctggatgt
     7321 cctcctcgcg gcacaccatc tgccaggcct tggtccacag cttctcggct tccagctcgt
     7381 ggaaggcgcg cgagctgtag cgctcgaccg acaggtcctc gtcgctgaag ccggggtgat
     7441 tcggctgcag atgctccgga accggcacgg tttccagctt caacaactgc tggaacaggt
     7501 tgggatcgcc cacctcctcc ggcggcaata cggtgaactt ggcgtgcttc ggattgttca
     7561 tgctagtgcc ctcgttattt ttattgagct ggtcgagcgg atggttacgg cgcttcgaga
     7621 atggtcacgg cgctgacacc cggcgcaccg tagacatggg tgtagccaag tcgcggccgg
     7681 cccggcacct gccgggcgcc ggcctgctgg cgcagctggg tgcagacttc gatgacctgg
     7741 cgcaggccgg aggcgcccac cggctcgcca ttggccaggc agccaccgtc ggtattcacc
     7801 ggcaggcggc cgccgatatc ggtttcgccg cggcacagca gatggccctg ctcgccgtgc
     7861 tcgcagaagc cgttttcggc gagatggatg atctccgagc cgctgtcggt gtcctgcagc
     7921 tggatcacgt cgatgtcgga cggaccgacg cctgccagct cgaaggccgc ccgcgaagcg
     7981 gtgaccgtcg gcgccggcga ctgctcggcc gccaggcccg gactgaatac ctcgaaggag
     8041 ccgtgatgac gggtacgcat caccgccgcg cgcaccagca ccgggttgcg gctgtacttg
     8101 tgcgcctcgc tcgcccggca cagcaccagc gccgcaccgc cctcggccgg attgcagaac
     8161 atgtacttgc gcagcggcag gttgaccggc atcgactcgg cgatctcggc gaagctcagc
     8221 tcgcgctggc gccaggcatg gggagtcagg gcgccgttgc ggaacgcgcg ctgggcgacg
     8281 tggaccagcg cctcctcggc gatgccgtgc tcgtgcaggt agcgctgggt cttgagggcg
     8341 aagaactggg tggtcagcat caggccggtt tcgccgtacc agtcctcgag gccccagtcg
     8401 gccggcttcg ggttgaaggc gccacgcggg tgcttgtcga agcccaccgc cacgccgatc
     8461 tcggccatgc cgctgcgcag cgcggcaatc acgctggcca gggcgctgcc gccggtggcg
     8521 cagccgttgg ccacgttgac gaacgggatg ccggtcaggc cgaggtggtt ggccagcgcg
     8581 tcggcattgc cgccatcctg gctgccgccg taggccacgc cgacctgatg ccactcgacc
     8641 cccgcgtccc tgagcgcctc gcgcaccgca taggcaccct gggcctggcc ggacaggccg
     8701 tcggtacgcc cgaaggggtg caggccgacg ccgatgatgg cgatatcttc catcactcgt
     8761 ctccttccgc ggcaagcggt gcaaaggcga aggtatgcag cggctcgccg actgcgttgc
     8821 gaccgtagcg gaccagggtg agctccatcg gcatgccgat gtgcagctgc tggggatcgg
     8881 cgacggtcag gcggctttcg accttgaccc cgcacggcat ctccacataa cccacaccgt
     8941 agggctggaa atcgacttcg ctttcgccgc tgttgtaggg cgccttgggc aggaagccct
     9001 ggatggtcca ggaccacagc cggccttggc gaccgatctc gcagcgctcg aactcggtgg
     9061 cgctgcagcg ggtgcagttg tcggcgatgg ggaagtagca ctctccgcac tggcggcagc
     9121 gactgcccag caggatcggg ccctgcgcgc cagcgcggag caggtgcccg ccggcgaggt
     9181 tggtctgcat caggtcgctc attgttgttt tcaacctttt ttaaaccatc aagattgttt
     9241 ttatttaagc gcagagtgac aaagcgcaga cggtttggca accgtctgcg cagaatttaa
     9301 ttggcttgtg cttgcagctg gaccttgcgc ttgagggctt ccatgtactc ggccatggcc
     9361 gtcttggcca gcaactcgtg cagccggtgc aggcggtcgc cgtgcaggta gctggacggg
     9421 tccagggcca cgccgcgcag ggcatgcatg atcagatcgc gcagacattc accggtttcg
     9481 cgcatgccgg cctcggccca gatcgggaag tgggcctcga tgctttgcga gatgcgctcg
     9541 tccagcgact gctccagggg agccatcacc gcatggagct cggcatcggt tcgcgcaccg
     9601 cgaaccagct cgtgaaacgc taggaaggag ggctgcatga agtactcctg aagcaccttg
     9661 agggtctcca gcatgctttc ctgggttggc tgaccaccgg cacgccgact gacctcgcgg
     9721 attgcggcgt cgtactccct gacccggata tcggtgatgt actcggctgc cgcggcaaac
     9781 acctcggcgc gcgacttgaa gtgatgggtc atggccccac gggatacgcc cgcctgcttg
     9841 gcgatcttct ccgtggtggt ctgggtatag ccgatgccga tcagacactg catggtggca
     9901 tcgagaatct gcgtgcgcat caaatggctt ttctcggctt gccaactgcc gtgctctgat
     9961 cgtttgcgcc gaacaggttg ttgcatatcg ccttcccctg aacctacaga aatatacccg
    10021 ttcattttat tctaccttca acattggaaa agcgggggac atgatccatc agaagattgc
    10081 ggacacgtcc cctgctcgcc cgcgattacc catcaggaca gacgaatcga atgtcctgca
    10141 tccacggtca cgacgctgcc actcatatag gaagaagcgt tgctggccag cagcagcaac
    10201 ggaccatcca gctcttcatg gcggccaagg cgcttgggcg gcaggctggc gatttcgcgc
    10261 ttgccgactt ccgactcata gaagggattg gtcatttcgc tggggaaata ccccggggcc
    10321 agagcgttga ctctgatgga gtagggtatc agatccagcg ccatgtactg agtcatgtga
    10381 atcaggcccg ccttggttgt gccatagttc acaaacttgt ccttggccag atggcccagg
    10441 atcgaggcga tattgatgat cgagccgggt ttgccggcaa cgatcaagcg cttagccatt
    10501 tcctgagcca cgaagaacgg gccggagagg ttggcatcca gcaaggacgt ccactgcgtg
    10561 cgactcattt cctcgaaggg cgccggatcg gacagcccgg cattgttgac cagcacgtcg
    10621 atcaggccaa gctcgcgctc gatactgtcg caggcctgct tgatggaggc gccatccgtg
    10681 acatccagta cacaggcgaa agcctcaccg ccctgggcgc ggatatccgc cgccaccgcc
    10741 tcgacccggt ccagacgccg cgccacgcag gccaccttgg cccctgccct ggccagaacc
    10801 cgggcaaagt gggcacccag cccggtgcct gctccggtca ccagggctac gcggccctcc
    10861 agagagaact ggtcctgcac gtttgcactg ttgttattgt tcattgcttg cactccatta
    10921 cccttcagac cagcagcgtg ggatctgcca gttgctcggc gacgaaacgg cagaagcgcg
    10981 ccgcatcggc cccattgatg acccgatggt cgtagctcag cgacagcggc aacagatgct
    11041 tcagcaccac ctggccatcc accagcg
//
LOCUS       DATCUN010000071        25732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_40825, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000071 DATCUN010000000
VERSION     DATCUN010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 25732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25732
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40825"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..841
                     /locus_tag="VIO83_02775"
     CDS             341..841
                     /locus_tag="VIO83_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY8330238.1"
                     /translation="MDCITRLAGQLGEALLSRQLQVSTAESCTGGGIAEAITRIAGSS
                     QWFEAGYITYSNRQKSQQLGVPETLFDTVGAVSREVVEAMARGALQRSGAQLAVAVSG
                     IAGPDGGTLDKPVGTVWLAWQDGEHGFSSRQQFAGDRQAVREQTVVCALRGLLRLAAG
                     EDPVEG"
     gene            927..1976
                     /gene="recA"
                     /locus_tag="VIO83_02780"
     CDS             927..1976
                     /gene="recA"
                     /locus_tag="VIO83_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY8330239.1"
                     /translation="MDENKKRALAAALGQIERQFGKGAVMRMGDHERQAIPAISTGSL
                     GLDIALGIGGLPKGRIVEIYGPESSGKTTLTLSVIAEAQKLGATCAFVDAEHALDPDY
                     AAKLGVNVDDLLVSQPDTGEQALEITDMLVRSNAVDVVIIDSVAALVPKAEIEGEMGD
                     THVGLQARLMSQALRKITGNIKNANCLVIFINQIRMKIGVMFGNPETTTGGNALKFYA
                     SVRLDIRRIGAVKEGDEVVGSETRVKVVKNKVAPPFRQAEFQILYGKGIYRNGEIIDL
                     GVQQGLIEKSGAWYSYQGSKIGQGKANAARYLEDNPEVRQTIEDAIRAQLLGSPANLK
                     ALAASEEDVDAEVDI"
     gene            1983..2444
                     /gene="recX"
                     /locus_tag="VIO83_02785"
     CDS             1983..2444
                     /gene="recX"
                     /locus_tag="VIO83_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEY8330240.1"
                     /translation="MAVMLDSPVAVRRAAMDLLARREHSRVELARKLRQRGAAVELID
                     SALERLSEEGLLSDERFLESFIRSRAQAGYGPLRIRDELNQRGLPRAVVERALAESPI
                     DWATNLRELWRRRFAALPADAREKAKQGRFLLYRGFAHDAVSRLLRGGGEE"
     gene            complement(2517..3605)
                     /locus_tag="VIO83_02790"
     CDS             complement(2517..3605)
                     /locus_tag="VIO83_02790"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEY8330241.1"
                     /translation="MSYESDDYLSRHFQTSGIDVGLKVDELVSISVPPGSPNQALYRE
                     MLMTVVRMAEADRNRWDAKIMMQTLREMENAFSMLEQFKRRRKVTVFGSARTQADHPL
                     YLLARDLGRLLTHHELMTVTGAGGGIMAAAHEGAGLEHSLGLNISLPFEQRANDVVHG
                     TGNLLPFHFFFLRKLFFVKEADALVLCPGGFGTLDEALEVLTLIQTGKSPIVPVVLLD
                     APEGRYWKDALHFIREQLEENGYILPSDMNLLKLVTSAEEAVVEITHFYRNYHSSRWL
                     KGLFAIRMNRPLNAHALAYLHEEFTDLCLEGSFAQQPYSELEQDEPEFSHLVRLAFHF
                     NGRDHGRLRELINFINMPENWQRELPHV"
     gene            complement(3634..5040)
                     /locus_tag="VIO83_02795"
     CDS             complement(3634..5040)
                     /locus_tag="VIO83_02795"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8330242.1"
                     /translation="MALLTFLGAIQQVTGSCYLIETRDGTRVLLECGMRQGRREDEAH
                     NSEAFPFDPLKLDAVVISHAHIDHSGLLPRLAAQGYRGPIYATDATCDLLQLMLMDSA
                     HIQENDAEWENKWRARLGREPVVPLYTEADAELALKLRRPVLYGNKVEVARGISVTFH
                     DAGHILGSAIVELEVHDHGLTRRMVFSGDLGNTCTPLMRDPTPLSEADVVLMESTYGD
                     RDHRCDEDTLEELADILQQAHRDGGNVLIPAFAVGRTQDLIFHLGRFYQQGRVPQQAV
                     FLDSPMAIQANAIYHRFHEQLDPKDRAEMRSNGGGPRVEDWLPILRCTPKAEDSMAIN
                     RIQSGAIIIAGSGMCNGGRIVHHFKHNLWRKECHIVFPGFQAQGTLGRAIVDGAPTVR
                     VLRQRIAVKAQVHTLGGFSAHAGQSQLIDWVKHFDHRPELYLVHGELEKMKVLQQALH
                     DRLQWTANIPEPGEQIAL"
     gene            5164..7569
                     /locus_tag="VIO83_02800"
     CDS             5164..7569
                     /locus_tag="VIO83_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose 5-phosphate 3-epimerase"
                     /protein_id="HEY8330243.1"
                     /translation="MPQLLPSAEELHAHALAEPAFAEWARGCGPLEHRPATQAAVFRL
                     AHRLVQAGQQPDLAGVYRLLQALDRLTCTGLWLVAHMTYARRVRLDGAPLLAEDFKAR
                     PEGHTGGALNMVPAYAGYLALNALSGQTRGWTMGQGHCVAAIEALNVLLGNLHPEQAA
                     RYGCDEAGLSRLVADFYSYAQASDGLPGVPLGSHVNPHTAGGIAEGGYLGFAELQYAH
                     QPLPGETLVAFLSDGAAEEQRGSDWMPRWWRAEDCGVALPVMIANGRRIEQRTELGTA
                     EGLADFAEHLRHCGFDPMHFDGRDPAAFVCTLHEMEQHLAHRVAELARGILHYPLPIP
                     YAIAETVKGFGFYGAGVNAAHNLPLPGNPHVDAEARRLFNESAAALWVAPDELQQAVD
                     CFAALRGGRPLERDHALAVRHPALPRLPELHFHAQACSPMSAIDRFFVDLVAANPQLR
                     PRVGNPDELASNRLGGVLQALKHRACAPENDSEALDGAIITVLNEEAVVSACLANQGG
                     LNLVASYEAFCVKMLGAVRQTLTFARQQREVGRAAGWLGWPLVATSHTWENGKNQQSH
                     QDPSFCEALLGEMSDGIRVLFPADHNSTLALLPQIYQSRGRLACMVVPKRERPAVFNA
                     EQARQLAHDGAIVVAERVGKEPLLLIANGSYQLTEMQRAARRLEEAACAYRLIYLQEP
                     ARFRAPRDLLEQQVSADEALRERLFPSALRRRVLLTHMRPEVARGHLWPILPDASQCA
                     ALGYRNRGGTLDGAGMLFANRACWGNVVAACARLLDMPRTALLTAEEAAALAGKGDPA
                     ILR"
     gene            complement(7634..8035)
                     /locus_tag="VIO83_02805"
     CDS             complement(7634..8035)
                     /locus_tag="VIO83_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004397588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing-regulated virulence factor family
                     protein"
                     /protein_id="HEY8330244.1"
                     /translation="MRRIYVLLAGLLAASATQAASLQDFELNKLLEDVARQSSVGTPR
                     AINADILDQGYTVEGNQLINHLSVRASHATEMRGNPDAVRQQLRASVCRNPGYRQLLS
                     KGAVLSYAFSEHKSNRPVATERFQSSDCGLK"
     gene            8247..8960
                     /locus_tag="VIO83_02810"
     CDS             8247..8960
                     /locus_tag="VIO83_02810"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016562190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HEY8330245.1"
                     /translation="MSNSVARLRGERLARSTKPFVTRGSRMPRCPQCRLEQSYCMCAW
                     RPRVPARAGVCLLMHDIEALKPSNTGWLIADVVADNSAFSWSRVEVDERLLALLDDPQ
                     WQPYIIFPAEYAAPERVVETVAPASGKRPLFILLDATWTEARKMFRKSPYLDRFPVLS
                     LTPEQHSRYRLRRSTRDEHLCTAEVAALCLELAGDSRAAEALDAYLDVFSERYLGFKR
                     SLPLDEQSAAHQRLRPFVA"
     gene            9219..9869
                     /gene="erdR"
                     /locus_tag="VIO83_02815"
     CDS             9219..9869
                     /gene="erdR"
                     /locus_tag="VIO83_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ErdR"
                     /protein_id="HEY8330246.1"
                     /translation="MAAYEILIADDHPLFRSALQQALSMGLGDEAHLVEVASIAELET
                     RLNEKRDWDLVLLDLNMPGAYGFSGLVLLRGQYPQIPVVMVSAQEEAAVVQRSREFGA
                     SGFIPKSSPLETIQQAVRAVLDGDAWWPPQLEEAAALSEEARAASEGLASLTPQQFRV
                     LTMVCEGLLNKQIAYELSVSEATVKAHVTAIFRKLGVRTRTQAALLLQQMESMPAA"
     gene            9986..10354
                     /locus_tag="VIO83_02820"
     CDS             9986..10354
                     /locus_tag="VIO83_02820"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020304558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEY8330247.1"
                     /translation="MLSPFKGQTGLKRILNAAGYSWDGLRAAFGGEAAFRQLVLLNAV
                     LVPLAFFLDVSRGERAILVAVCLLALIVELLNSAVEAAIDRISLERHPLSKNAKDMGS
                     AAQLVALSMIGLVWGIVLLG"
     gene            complement(10367..11293)
                     /locus_tag="VIO83_02825"
     CDS             complement(10367..11293)
                     /locus_tag="VIO83_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8330248.1"
                     /translation="MRFTLRQLEVFVAVARLESVTRAAESLALSQSAASTSLAELERH
                     YDSPLFDRIGKRLALSALGEELLPKAVALLDRAGEIEQLLQGHSGFGSLQVGATLTVG
                     NYLATLLIGHFMQRHPECRVRLHVQNTSHVVQGVAQHNLDLGLIEGDCQHTDIHVQPW
                     IEDELVVFCAPQHPLARRGTASLEELLQEAWILREQGSGTRLTFDQAMRHHPSSLNIR
                     LELEHTEAIKRAVESGLGIGCISRLALRDAFRRGSLVAVETPELDLRRQFCFIWHKQK
                     YQSSAMREFVELCRSFTAGMQRSDQIHLQEIP"
     gene            11421..12197
                     /gene="fpr"
                     /locus_tag="VIO83_02830"
     CDS             11421..12197
                     /gene="fpr"
                     /locus_tag="VIO83_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-NADP reductase"
                     /protein_id="HEY8330249.1"
                     /translation="MSNLMSERVVSVHHWNDTLFSFKTTRNQGLRFENGQFVMIGLEA
                     NGRPLMRAYSIASPNYEEHLEFFSIKVPDGPLTSRLQHLQPGDELVVSRKPTGTLVLG
                     DLLPGKHLYLLSTGTGLAPFMSVIQDPETYERFDKVILVHGVRWVSELAYADFITNEL
                     PNNEFFGEAVREKLIYYPTVTREPFRNQGRQTDLMRSGKLFSDIGLPPINPQDDRAML
                     CGSPSMLKETSEVLDSFGLKVSPRMGDPGDYLIERAFVEK"
     gene            complement(12344..13042)
                     /gene="tsaA"
                     /locus_tag="VIO83_02835"
     CDS             complement(12344..13042)
                     /gene="tsaA"
                     /locus_tag="VIO83_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003198370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEY8330250.1"
                     /translation="MPYSVSPVGYLRSCFKEKFAIPRQPQLAPAARGVLELVAPFDTG
                     EAVAGLEQVSHVWLLFLFHQALEDTPRLKVRPPRLGGNRSLGVFASRATHRPNGIGQS
                     VVKLERVEPGRLHLSGIDLLDGTPILDIKPYVPYADIVSEAHNAIADAPPPAIPVEWS
                     DTTLQQAREHALRLAEPVVELIEQCLAQDPRPAYQVPTPERRYGVLFWDVQVRWHYPE
                     PGRIRVLEVVHDQP"
     gene            complement(13164..14372)
                     /gene="trpB"
                     /locus_tag="VIO83_02840"
     CDS             complement(13164..14372)
                     /gene="trpB"
                     /locus_tag="VIO83_02840"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY8330251.1"
                     /translation="MSASHRFAPMPDARGYFGSYGGQLVPPHLKQVMDEIDAAYEEIR
                     QREDFQQELASLFADYVGRPSPIFHARRLSEKLGGAQIHLKREDLNHTGAHKINHCLG
                     EALLAKFMGKSKVIAETGAGQHGVALATACALVGIPCEIHMGQVDIEKEHPNVTKMKI
                     LGCTLVPVTRGAATLKEAVDSAFEEYLKDPHNFIYAIGSVVGPHPFPKMVRDFQSIIG
                     TEARAQFLAKHGRLPDHVTACVGGGSNAMGMFTAFLDDEAVKLVGVEPAGEGLDKPGR
                     HSATLSMGKPGELHGMACYVLEDAEGHPAAVHSIASGLDYPGVGPQHSYLKDIGRVSY
                     QTASDQECLDAFMTLSRVEGIIPALESAHAVAWAMRVAPTLAKDQHILVNLSGRGDKD
                     ADYVAKLLGL"
     gene            complement(14507..15181)
                     /locus_tag="VIO83_02845"
     CDS             complement(14507..15181)
                     /locus_tag="VIO83_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8330252.1"
                     /translation="MQQLKLISSRNEAGNIKSFVFETGGLTWTAGQNQAFVLPQAGAD
                     EADNLRWFTLASAPSEQVVQVSTRVGPSAFKQALDRLQPGDSIGVRDLGGDFTWEEES
                     DTPVVLVAGGIGVTPFRSMLMERDARGKPLNAILLYFSRDAQIAFRDELDQLAARHPE
                     LDVRYLIGELISAERILDEEPLATQLPVYLSGPEPMVESVGNALKQRGVSLRQDWFPG
                     YDERNY"
     gene            complement(15248..15964)
                     /locus_tag="VIO83_02850"
     CDS             complement(15248..15964)
                     /locus_tag="VIO83_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation transporter"
                     /protein_id="HEY8330253.1"
                     /translation="MNVWLHILGITLLPGLAMPLGALIASREHVHPRWLETELRHAVL
                     AFGAGALLAAVALVLVPEGMRQLPVWLTTLCFAAGGVAFMQIDRLLAAHRTTASQAID
                     MLFDYLPETLALGATVALGSPAGLLLALLMALQNLPEGFNAYRELHRSSHLANWRIIG
                     LFTLIALLGPLAGVCGYLWLAPYPQVVGAIMLFASGGILYSVFQDIAPQVPLQRHWAP
                     PLGAVAGFLLGMIGQLLTAQ"
     gene            complement(16081..17406)
                     /gene="rimO"
                     /locus_tag="VIO83_02855"
     CDS             complement(16081..17406)
                     /gene="rimO"
                     /locus_tag="VIO83_02855"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY8330254.1"
                     /translation="MSTATPKVGFVSLGCPKATVDSERILTQLRMEGYQIVPSYEDAD
                     VVVVNTCGFIDSAKAESLDAIGEALAENGKVIVTGCMGVSEDSIRNVHPSVLAVTGPQ
                     QYEQVVNAVHEVVPLSTSHDPFVDLVPPQGIKLTPRHYAYLKISEGCNHSCAFCIIPS
                     MRGKLVSRPVGEVLSEAERLVKAGVKELLVISQDTSAYGVDLKYKLDFWNGQPVKTRM
                     LELCEELGKLGVWVRLHYVYPYPNVDDVIPLMAAGKILPYLDIPFQHASPKVLKAMKR
                     PAFEDKTLARIKQWREICPELTIRSTFIVGFPGETEEDFQYLLDWLTEAQLDRVGCFQ
                     YSPVEGAPANDLGLEPVADEIKQERWERFMAHQQAISAARLQLKIGKELEVLIDEVDE
                     EGAIGRSAADAPEIDGNVYVDSEQPLKPGDKVRVRVTDADEYDLWAEVI"
     gene            complement(17555..18097)
                     /locus_tag="VIO83_02860"
     CDS             complement(17555..18097)
                     /locus_tag="VIO83_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY8330255.1"
                     /translation="MSTASSRYSPINIALHWVMLLLIAAVYACIELKGNFVRGSEIRE
                     GLKHWHFMLGLAIFALVWLRLLGRLIGKTPAIQPAPPSWQTGLSHLMHLALYVLMIGM
                     PLVGWLILNAEGKPAPFFGFELPMLVQKDHGLAESLEGLHEFGGIAGYWLIGLHAVAG
                     LLHHYVIRDNTLSRMLPARG"
     gene            complement(18239..18315)
                     /locus_tag="VIO83_02865"
     tRNA            complement(18239..18315)
                     /locus_tag="VIO83_02865"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18279..18281),aa:Met,seq:cat)
     gene            18568..19617
                     /gene="dinB"
                     /locus_tag="VIO83_02870"
     CDS             18568..19617
                     /gene="dinB"
                     /locus_tag="VIO83_02870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY8330256.1"
                     /translation="MRKIIHIDCDCFFAAIEMRDDPRLAGRPMAVGGAPDRRGVIATC
                     NYEARAFGVRSALSSRQALALCPQLLIVRPRFEAYREASLQIRSILQDYTELIEPLSL
                     DEAYLDVSDSAHFAGSATRIAQDIRRRVREQLEITVSAGVAPNKFLAKVASDWHKPDG
                     LCVITPAEVDEFVAVLPVARLHGVGKVTAERLARLGIQRCGDLREWQRLALVREFGSF
                     GERLWQLARGIDERPVQVDRRRQSVSVEHTFDRDLPDLAACTAELPALLEQLERRLTR
                     LDSGYRVGKPLVKLKFHDFTQTTLEQAGVSRDADSYRRLLAAAFTRGGKPVRLIGVGV
                     RLLDLREGGEQLELF"
     gene            complement(19619..20035)
                     /locus_tag="VIO83_02875"
     CDS             complement(19619..20035)
                     /locus_tag="VIO83_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEY8330257.1"
                     /translation="MLHRTALVTRPTVLALALTGCASAPLALEQERTYRVEWIDERPL
                     LEGSHLTITFGADDRAYGTGGCNHWFAGYGVEGEILRFSQIGSTRKACAPALMEQEAR
                     FFQALEHVRRWDLSPLEQLRLWPEQGKPIRLQPEEG"
     gene            complement(20035..20472)
                     /locus_tag="VIO83_02880"
     CDS             complement(20035..20472)
                     /locus_tag="VIO83_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330258.1"
                     /translation="MTEHDFRYNLLNPQHTLTECRALAPGRYQVTGNGGSIQAGDGLL
                     VTLKGSRDLSMRLVVEKVRHLINPPGQWLAVAKGPVFNELSILQWGVDCNGCGAHLDF
                     EFAVDAKLGAAARTPAAEARITELGWRHQAGHHLCPRCIAKEA"
     gene            complement(20520..21509)
                     /locus_tag="VIO83_02885"
     CDS             complement(20520..21509)
                     /locus_tag="VIO83_02885"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEY8330259.1"
                     /translation="MRITLFSSKPYDRDSFATANQPHGHELHFLDARLDADTAALAAG
                     SAVVCAFVNDDLSAPVLERLRAGGTRLLALRSAGYNHVDLSAAQALDLPVVRVPAYSP
                     HAVAEHAVALVLALNRRLHRAFNRTREGDFSLHGLTGFDLHGKCVGVVGCGQIGAVFA
                     RIMLGFGCRVRLHDPQPDAALEALGARFVALDELLASSDIISLHCPLNPATRHLIDGA
                     SLQRMKRGAMLINTGRGALVDTPALIDALKSGQLGYLGLDVYEEEADLFFEDRSDLPL
                     QDDVLARLLTFPNVIVTAHQAFLTREALAAIATTTLDNIAAWQAGRAANRVQA"
     gene            complement(21524..24301)
                     /locus_tag="VIO83_02890"
     CDS             complement(21524..24301)
                     /locus_tag="VIO83_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8330260.1"
                     /translation="MFDKLGIKGRVLLLSLLPTSLLALVLGSYSTWTHLSQMHSQLVE
                     RGQLVIEQLAPLSARLLVARDIPLLERLAGQILEQPDLRAITLLSPQGERLVHAGPRM
                     RTSAPPRDPQQLSLVSSAEATRFLLPVYGLHRQLGGHAPVEEDGRLLGWIELELSHQG
                     TLLQGYRSLLTSLLLTVAGLIVTALLALRMSRTISRPLQQISHGVAQIRDGQLQTRLP
                     ALGSHELDEVASGINRMAESLQSAQEELQHNVEQATEDLRNTMETIEVQNIELTMARK
                     EALEASRIKSEFLANMSHEIRTPLNGILGFTNLLHKTGLDSRQQDYLLTIQKSADNLL
                     AIINEVLDFSKIEAGKLVLEQAPFNLRDLIQDTLTILAPAAHEKNLELVCLIYRDTPQ
                     ALVGDALRLKQVLTNLVSNAIKFTHSGSIVVRAMLEEDLVSHAQLRISVQDSGVGLSE
                     SEQRSLFQAFNQVDNSSSRQAGGTGLGLVISKRLIEQMGGEIGVTSEKDEGTEFWLQL
                     TLPKAAEERDELPRALLGGRCAALLESHQLARQSLRHQLEDCGLQVIEFANLDALIEE
                     AALRQHGSAPLAIALIGINAREHPPERLSRQLWELNQQGCQALLLCPTTEQALYHASL
                     PESHGQLQAKPACTSKLQRTLIDLLSPRVQRVEASGATNPAASRAPRLLCVDDNPANL
                     MLVQTLLGDMGAQVKAVDNGYDALRAVQQETFDLVFMDVQMPGMNGRETTEAIRQWES
                     EHGQPPLPIVALTAHAMANEKRALLQCGMDDYLTKPISERQLSQVVLKWTGLALHNGE
                     VREPLLPERGSAATELPILDPAEGLRLSAGKADLAADMLSMLLDSLPSDQAVIRQARA
                     SNDSTALLERIHRLHGATRYCGVPQLRAACQRSESLLKQNAPANVALDELDAAITRLA
                     EQAARPVPLPT"
     gene            24522..25427
                     /gene="cysM"
                     /locus_tag="VIO83_02895"
     CDS             24522..25427
                     /gene="cysM"
                     /locus_tag="VIO83_02895"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HEY8330261.1"
                     /translation="MTVQYPTIADCVGNTPLVRLQRLPGKTSNTLLVKLEGNNPAGSV
                     KDRPALSMIGHAELRGDIQPGDTLIEATSGNTGIALAMAAAIKGYRMILIMPDNMSAE
                     RKAAMSAYGAELIQVSKAEGMEGARDLALKLQAEGRGKVLDQFGNQDNPQAHYESTGP
                     EIWRQTGGTITHFISSMGTTGTIMGTSRYLKEQNPAVQIVGLQPQEGAAIPGIRRWPQ
                     EYLPKIFDASRVDRVIDMAQGEAEDVMRRLAREEGIFCGVSSGGAVAAMLRLSQEVEN
                     AVMVAIICDRGDRYLSTGVYDFPQG"
     gene            25512..>25732
                     /locus_tag="VIO83_02900"
     CDS             25512..>25732
                     /locus_tag="VIO83_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEY8330262.1"
                     /translation="MAKRNTGLRFQPSGGVRTAQLPVGKKQLLSIERLAHDGRGIAHE
                     GGRTWFVAGALAGEQVEARVLAARSQVVEA"
BASE COUNT         4083 a         8209 c         9065 g         4375 t
ORIGIN      
        1 gggggggggg ggaggactct aagccagcgg cctaacggct tcaagatgcg aggctattgt
       61 ctgagaggct ggcattgcgc ctggtgattg atggggcatt tttggggcaa accgaacgcc
      121 aaaccatgcc aaccaatgcc aattcggcta catgcgcgag gtgtgcgatt cgtcgcgaaa
      181 gccgcgccgg ctgcggcctt ccgctggttt tcagcgtgct acacctacac aatcggcgtg
      241 tgggcggaaa aatcgctgtt gctcatcgcg gtccggcttc aagtcgcaaa agaagctagt
      301 gtacggtatc gcccggacag gcttaacccc ggagacactc atggattgca tcacccggct
      361 ggcgggacaa ttgggcgaag cattgctgag caggcagttg caggtcagta ccgccgagtc
      421 ctgcaccggc ggcggtatcg ccgaggcgat cacgcgcatc gccggcagct cgcagtggtt
      481 cgaggccggc tacatcacct attccaatcg gcagaagagc cagcagcttg gggtgcccga
      541 gacgctgttc gacactgtcg gcgcggtcag tcgcgaggtg gtcgaggcca tggcccgggg
      601 tgctttgcag cgcagcggcg cccaactggc ggtggcggtc agcggtatcg ccgggccgga
      661 tggcgggacc ctggacaagc cggtgggcac cgtctggctg gcctggcagg atggcgagca
      721 cggtttcagt tcccgccagc agtttgccgg tgaccgccag gcggtacgcg agcagacagt
      781 ggtctgcgcg ctgcgcggct tgttgcgact ggcggcaggg gaagatcctg tcgaggggta
      841 ggcgggtgcc ttaccctgtg aaataatact ggctaattat acagttgatc gtggccggct
      901 ggccgctctg aaacgagagg acttcaatgg acgagaacaa gaagcgtgcc ctggccgcgg
      961 ccctgggtca gatcgagcgt cagttcggta agggcgcggt catgcgcatg ggcgaccacg
     1021 agcgccaggc gatcccggcc atttccaccg gttcgctggg tctggatatc gccctcggta
     1081 tcggcggtct gcccaagggc cggatcgtcg agatctacgg tccggagtcc tcgggcaaga
     1141 ccaccctgac cctgtcggtg atcgccgagg cacagaagct gggcgccacc tgcgcctttg
     1201 tcgatgccga gcacgcgctg gacccggact acgccgccaa gctcggtgtc aatgtcgacg
     1261 acctgctggt ctcgcagccg gacaccggcg agcaggcctt ggagatcacc gacatgctgg
     1321 tgcgctccaa tgcggtcgat gtagtcatca tcgactccgt ggcggcgctg gtgcccaagg
     1381 cggaaatcga gggcgagatg ggtgacaccc atgttggcct gcaggcacgt ctgatgtccc
     1441 aggccctgcg caaaatcacc ggtaacatca agaatgccaa ctgcctggtg attttcatca
     1501 accagatccg catgaagatc ggcgtgatgt tcggcaaccc ggaaaccact accggcggca
     1561 atgcgctgaa gttctacgcc tcggtacgcc tggatatccg ccgtatcggt gcggtcaagg
     1621 aaggcgacga ggtggtcggc agcgaaaccc gggtcaaggt agtgaagaac aaggtggccc
     1681 cgccgttccg tcaggcagag ttccagatcc tctatggcaa aggcatctat cgcaacggcg
     1741 agatcatcga cctcggtgtg cagcaggggc tgatcgagaa atccggcgct tggtacagct
     1801 accagggcag caagatcggc cagggcaagg ccaatgccgc gcgctacctc gaggacaatc
     1861 cggaggtgcg tcagaccatc gaggatgcca tccgcgccca gctgctgggc tcgcccgcca
     1921 acctcaaggc gctggccgcc agcgaggaag atgtcgacgc ggaagtggac atctaagcga
     1981 gcatggcggt catgctcgac agccccgtgg ccgtgcggcg ggcagccatg gatctgctcg
     2041 ccaggcgcga gcacagccgt gtcgagctgg cgcgcaaact gcgccagcgt ggggccgcgg
     2101 tcgaactgat cgacagtgcc ctcgaacgac tgagcgaaga ggggctgctc agcgacgagc
     2161 gctttctgga aagcttcatt cgcagtcggg cgcaggccgg ctacggcccg ctgcgtatcc
     2221 gcgacgaact gaaccagcgc ggcctgccgc gtgcggtcgt cgagcgggcg ctggcggaga
     2281 gcccgatcga ctgggcgaca aacctgcgcg agctgtggcg gcgacgcttt gccgctctgc
     2341 cggccgatgc ccgcgagaag gcgaaacagg gccgtttcct gctttatcgc ggctttgccc
     2401 atgacgcggt gagtcgcctg ctgcgcggtg gcggcgagga ataatccggt ctccgcatga
     2461 aaaacgcccc ggctcatcgc gagctcgggg cgttttgctg tagggcggat agccgctcag
     2521 acgtgtggca gctcgcgctg ccagttctcc ggcatgttga tgaagttgat cagctcgcgt
     2581 aggcggccgt gatcgcgacc gttgaagtgg aaggccaggc ggaccaggtg gctgaactcc
     2641 ggttcgtcct gctccagctc gctgtacggt tgctgggcaa agctgccctc caggcacagg
     2701 tcggtgaact cctcgtgcag gtaggcgagg gcatgggcgt tgagcggccg gttcatgcgg
     2761 atggcgaaca gccccttgag ccagcggctg gagtggtagt tacggtagaa gtgggtgatt
     2821 tccaccaccg cctcttcggc cgaggtgacc agcttcagca ggttcatgtc gctgggcagg
     2881 atgtagccgt tttcctccag ctgctcgcgg atgaagtgca gcgcatcctt ccagtagcgg
     2941 ccttccggcg cgtcgagcag caccaccggc acgatcgggc tcttgccggt ctggatcagg
     3001 gtcagcactt ccagcgcctc gtccagagtg ccgaagccgc ccgggcagag caccagggca
     3061 tcggcctcct tgacgaagaa cagcttgcgc aggaagaaga agtggaaggg cagcaggttg
     3121 cccgtgccgt gcaccacgtc gttggcacgc tgttcgaatg gcagggaaat gttcagtccc
     3181 aggctatgct cgaggccggc accctcgtga gcggcggcca tgatcccgcc gccggcaccg
     3241 gtcactgtca tcagctcgtg atgggtcagc aggcgaccca ggtcacgggc cagcaggtag
     3301 agtgggtggt ccgcctgggt gcgcgcggag ccgaacacgg tgaccttgcg acggcgtttg
     3361 aactgctcga gcatgctgaa cgcgttctcc atctcgcgca gcgtctgcat catgatcttg
     3421 gcatcccagc ggttgcggtc ggcctcggcc atgcgtacca cggtcatcag catttcacgg
     3481 tacagggctt gattggggct tccgggcggc acgctgatgc tgacaagttc gtcgaccttg
     3541 aggccgacat cgatgccgct ggtctggaag tgtctcgaca ggtagtcatc cgactcgtag
     3601 gacatgtgag tactccttgt tgttatctgt cgctcagagt gcgatctgct cccccggttc
     3661 cgggatgttg gcagtccact gcaggcgatc atgcaatgcc tgttgcagca ccttcatttt
     3721 ttccagctcc ccatggacta aatatagctc cggtcgatga tcgaagtgct tcacccagtc
     3781 gattagttgc gactggccgg catgggcgga gaagcctccc agggtgtgta cctgggcctt
     3841 gacggcaata cgctggcgca gcacgcgtac ggtcggcgcg ccatcgacga tggcacggcc
     3901 gagggtgcct tgcgcctgaa agccggggaa cacgatgtgg cactccttgc gccacaggtt
     3961 gtgcttgaag tggtgaacga tacgcccgcc gttgcacatg ccgctgccgg cgatgatgat
     4021 cgcgccgctc tggatgcggt tgatcgccat cgagtcctcc gccttgggcg tgcagcgcag
     4081 gatcggcagc cagtcttcga cgcgggggcc gccgccgttg ctgcgcatct cggcgcggtc
     4141 cttggggtcg agctgttcgt ggaagcgatg gtagatggcg ttggcctgga tcgccatcgg
     4201 gctgtcgagg aacaccgcct gctgcggaac ccgaccctgc tggtagaagc ggccgagatg
     4261 gaagatcagg tcctgggtgc ggccgacggc gaaggcgggg atcagcacgt tgccgccgtc
     4321 gcgatgggcc tgttgcagga tgtcggccag ctcctcgagg gtgtcctcat cgcagcgatg
     4381 gtcgcggtca ccgtaggtcg actccatcaa caccacgtcg gcctcgctca gtggggtcgg
     4441 atcgcgcatc agcggggtgc aggtattgcc gaggtcgccg gagaacacca tgcgccgggt
     4501 caggccgtgg tcatgcactt ccagttcgac gatggccgag ccgaggatgt gcccggcatc
     4561 gtggaaggtc acgctgatgc cgcgcgccac ttctaccttg ttgccgtaca gcaccgggcg
     4621 acgcagcttg agcgccagtt cggcgtcggc ttcggtgtac agcggtacca ccggctcgcg
     4681 gccaaggcgt gcgcgccact tgttttccca ctcggcgtca ttttcctgaa tgtgcgcgga
     4741 atccatcagc atcagctgca gcaggtcgca ggtggcatcc gtcgcataga tcgggccgcg
     4801 gtagccctgc gcggccaggc gggggagcag gccgctatgg tcgatatggg catgggagat
     4861 caccacggca tccagcttca ggggatcgaa ggggaaggcc tcgctgttgt gggcttcgtc
     4921 ttcgcggcgg ccctgtcgca tgccgcactc aagcaggacg cgtgtgccgt cacgggtctc
     4981 gatcaggtag caggagccgg tgacctgctg gatggcgccg agaaaggtaa gcagagccat
     5041 gaaaaactcc ttgttgcgaa tgtcgcgacc gcctgccaag gggcggcagg gccttgatac
     5101 aaatcagctg tgatgctcca tgtcgttcta gactaccccc aagcctgcct tggagtcttg
     5161 ttcatgcccc agcttttgcc cagcgccgaa gaactgcacg cccatgccct cgccgagccg
     5221 gcctttgccg aatgggctcg gggctgcgga ccgctggagc accggccagc cactcaggcc
     5281 gcggtgttcc gtctggcgca tcgtttggtg caggctggcc agcagcccga tctggctggc
     5341 gtctatcgct tgctgcaggc gctcgatcgc ctgacctgta ccggcctgtg gctggtggcg
     5401 cacatgacct atgcccgccg cgtgcgtctc gatggtgctc cgctgctggc cgaggacttc
     5461 aaggcgcggc ccgaggggca taccggcggc gccctgaaca tggtgccggc ttatgccggc
     5521 tacctggcgc tgaacgccct gagtgggcag acccgcggct ggaccatggg ccaaggccat
     5581 tgcgtggccg ccatcgaggc gctcaacgtg ctgctcggca atctgcaccc ggaacaggcc
     5641 gcccgctacg gctgcgatga agccggcttg agccggctgg tggccgattt ctacagctac
     5701 gcccaggcct ccgacggcct gcccggcgtg ccgctgggca gccacgtcaa cccgcatacc
     5761 gcgggcggca tcgccgaggg tggctatctc ggttttgccg agctgcagta cgcccaccag
     5821 ccgctgccgg gcgaaaccct ggtggccttc ctctccgacg gcgccgcgga ggagcagcgc
     5881 ggcagcgact ggatgccgcg ctggtggcgt gccgaggatt gcggcgtagc cctgccggtg
     5941 atgatcgcca acggccggcg catcgagcag cgcaccgaac tgggcaccgc cgaggggctg
     6001 gccgactttg ccgagcatct gcgccactgc ggcttcgacc cgatgcactt cgacggccgc
     6061 gacccggccg ccttcgtctg caccctgcac gagatggagc agcacctggc acatcgggtc
     6121 gccgagctgg cgcgtggcat cctgcactac ccgctgccca tcccctatgc catcgctgaa
     6181 acggtgaagg gcttcggttt ctacggcgcc ggtgtgaacg cggcgcacaa cctgccactg
     6241 ccgggcaatc cgcacgtcga cgccgaggcg cgccggttgt tcaacgaaag cgccgcggcg
     6301 ctctgggtgg cgccggacga gttgcagcag gcagtggatt gctttgccgc cctgcgcggc
     6361 gggcgccccc tcgaacggga tcatgcgctg gcggtacgcc accccgcgct gccacgcctt
     6421 cccgagttgc acttccatgc ccaggcctgc tcgccgatga gtgccatcga ccgcttcttc
     6481 gtcgacctag tggcggccaa cccgcagctg cgtccgcgcg tcggcaaccc cgacgagctg
     6541 gccagcaacc gtctgggcgg cgtattgcag gcgctcaagc accgggcgtg tgccccggag
     6601 aatgacagcg aggcgctgga tggcgcgatc atcaccgtgc tcaacgagga ggcggtggtg
     6661 tccgcctgcc tggccaacca gggtggtctc aacctggtgg ccagctacga ggcgttctgc
     6721 gtgaagatgc tcggcgccgt gcgccagacc ttgaccttcg cccgccagca gcgggaggtc
     6781 ggccgtgccg ccggctggct cggctggccg ctggtggcca cctcgcacac ctgggaaaac
     6841 ggcaagaacc agcagtcgca tcaagatccc agcttctgcg aggcgctgct cggcgagatg
     6901 agcgacggca tccgcgtcct gttccccgcc gatcacaaca gcaccctggc gctgctgccg
     6961 cagatctacc agagtcgtgg ccggctggcc tgcatggtgg tccccaaacg tgagcgacct
     7021 gccgtgttca acgccgaaca ggcccgccaa ctggctcacg atggcgccat cgtggtggcg
     7081 gagcgggtcg gcaaagagcc gctgctgctg attgccaacg gcagctacca gctgacggaa
     7141 atgcagcggg cggccaggcg tctggaggag gccgcctgtg cctaccggct gatctacctg
     7201 caggaaccgg cacgcttccg cgccccgcgc gacctgctgg agcagcaggt cagcgccgac
     7261 gaggccctgc gcgagcgcct gttcccgtcc gccctgcgcc gccgcgtgct gctcacccac
     7321 atgcgtccgg aagtggcgcg gggccacctg tggccgatcc tgccggatgc cagccagtgt
     7381 gccgcgctgg gctaccgcaa tcgcggaggc acccttgacg gggccggcat gctgttcgcc
     7441 aaccgtgcct gctggggcaa cgtggtggcc gcctgcgcac ggctgctgga catgccgcgc
     7501 accgcgctgc tgaccgccga ggaggcggcg gcgctggccg gcaagggcga tccggcgatc
     7561 ctgcgctgag gcgccgaaga caacgctccg ccgctgcagg gcagcggcgg agcgttgctg
     7621 cctatggagg tgcctacttg aggccgcagt cgctggactg gaagcgctcc gtggctaccg
     7681 ggcgattgct cttgtgctcg ctgaaggcgt agctcagtac ggcgcccttg ctcagcaatt
     7741 ggcggtagcc gggattgcgg cagacactgg cgcgcaactg ctggcgtacc gcgtccgggt
     7801 tgccgcgcat ctcggtggca tgactggcgc gcacgctgag gtgattgatc agctggttgc
     7861 cttcgacggt atagccctga tcgaggatgt ccgcgttgat cgcccgcggg gtgccgacgc
     7921 tgctttgccg ggcgacgtcc tcgagcagtt tgttcagttc aaagtcttgc agtgaagcgg
     7981 cctgggtggc actcgccgcc agcaggccgg cgagcaggac atagatacgg cgcatggaaa
     8041 gtctcctgaa aaacctgcct gttcgacccc cgccagcacg gcaggttcgc atggccgcca
     8101 tcatacgcgc ctgcactgga tcgcgagcag ggctctgcta gactgccgcg ccttcgacca
     8161 accggccaat cccgtggagt tcgcatgctt cgatcctctg ttgcgccccg ctcgccgctg
     8221 ctacccgccg aaccggggag cggtcgatga gcaacagcgt cgcccgcctg cgcggggagc
     8281 gtctcgcccg cagcaccaag ccctttgtca cccgcggctc gcgcatgccg cgttgcccgc
     8341 agtgccgtct ggagcagagc tactgcatgt gcgcctggcg gccgcgggta ccggcgcgcg
     8401 ccggggtgtg cctgctgatg cacgacatcg aggccctcaa gccgagcaat accggttggt
     8461 tgatcgccga tgtggtcgcc gacaacagtg cctttagctg gtcgcgcgtc gaggtggacg
     8521 agcgtctgct ggcgctgctc gacgatccgc agtggcagcc ctacatcata ttcccggccg
     8581 aatatgccgc gcccgagcgc gtggtggaga cggtcgcgcc ggcgtccggc aagcgtccgc
     8641 tgttcatcct gctggatgcc acctggaccg aggcgcgcaa gatgttccgc aagagtccct
     8701 acctggaccg ctttccggtg ctcagcctga cgcccgagca gcactcgcgc taccgcctgc
     8761 gccgctcgac gcgcgacgag cacctgtgca ccgccgaggt ggcggcgctg tgcctggagc
     8821 tggccggcga cagccgcgcg gccgaggcgc tggatgccta cctcgacgtg ttcagcgagc
     8881 gctacctcgg cttcaagcgc agcctgccgc tggacgagca gagtgcggcg caccagcgcc
     8941 tgcgtccctt cgtcgcctga gccgggcccg cgtcggcgcc gtccaggcgc tgacagaacg
     9001 actggctcgc gggcactgcc aatgcctgcc gggctgctcg gaaaaaaagg cgaaaaccat
     9061 tcacaaaagg tagaattccg gaacttgcac ggcagggact tgtcgcccag ccaaagccct
     9121 acggcttttc cgggtacttt tcttgaccgg gggggagctg ctcggcatcc tgtgcgccgc
     9181 ttgagccccg cccgttcaga acaacaacag gatttatcat ggccgcctac gaaatactga
     9241 ttgccgacga ccacccgttg ttccgcagcg ccctgcagca ggcgttgagc atggggctgg
     9301 gggacgaggc gcatctggtg gaagtggcca gcatcgccga gctggagacg cggctgaacg
     9361 agaagcgcga ctgggatctg gtcctgctcg acctgaacat gccgggcgcc tacggtttct
     9421 ccggtctggt gctgctgcgt ggccagtatc cgcagatccc ggtggtgatg gtctccgccc
     9481 aggaagaggc cgctgtggtg cagcgctcgc gcgaattcgg cgccagcggc ttcatcccca
     9541 agtccagccc gctggaaacc atccagcagg ccgtgcgcgc ggtgctcgat ggcgatgcct
     9601 ggtggccgcc gcagctggaa gaagccgcgg ccctgtccga ggaggcgcgt gccgccagcg
     9661 aggggctggc cagcctgacg ccgcagcagt tccgcgtgct gaccatggtc tgcgaaggcc
     9721 tgctgaacaa gcagatcgcc tacgagctga gcgtttccga ggccacggtc aaggcccacg
     9781 tcacggccat cttccgcaag ctcggggtgc gtacccgcac ccaggctgcc ctgctgctgc
     9841 agcagatgga gtcgatgccg gccgcttgag cggcctgcat agcgggatag tcggcgcggt
     9901 cgacagcggc cgtcggcgtg gtttgacgtt tttttcaggc cgtctctggc agactggccc
     9961 gctttcagct tccggacgct tgcacatgct gtcgccattc aagggccaga ccggcctcaa
    10021 acgcatcctc aacgccgccg gctattcctg ggatggcctg cgtgccgcct tcggcgggga
    10081 ggcggcgttc cgtcagttgg tgttgctcaa tgccgtgctg gtgccgctgg ccttcttcct
    10141 cgacgtcagc cgcggcgagc gtgccattct ggtggcggtg tgcctgctgg cgttgattgt
    10201 cgagctgctc aactcggcgg tggaagccgc catcgaccgc atctccctcg agcgccatcc
    10261 gttgtcgaag aacgccaagg acatgggcag cgccgcccag ctggtggcgc tgtcgatgat
    10321 cggcctggtg tggggtatcg tcctgctcgg ctgaggcggt cggcgctcag gggatctcct
    10381 gcaggtgaat ttggtcgctg cgctgcatcc cggcggtgaa gctgcggcac agctcgacga
    10441 actcgcgcat ggcgctgctc tgatacttct gcttgtgcca gatgaagcag aactgccggc
    10501 gcaggtccaa ctccggggtt tccaccgcca ccaggctgcc acggcggaac gcgtcgcgca
    10561 gggccaggcg ggagatgcag ccgatgccca gccccgactc cacggcgcgc ttgatcgcct
    10621 cggtatgctc cagctcgagg cggatattca gcgaggacgg atgatggcgc atggcctggt
    10681 cgaaggtcag gcgcgtaccg gaaccctgct cgcgcagaat ccaggcttcc tgcagcagct
    10741 cttcgaggct cgccgtgccg cgccgggcca gcgggtgctg cggtgcgcag aacaccacca
    10801 gctcgtcctc gatccagggt tgcacatgga tatccgtgtg ctggcaatcg ccctcgatca
    10861 ggcccagatc gagattgtgt tgcgccacgc cctgcaccac gtggctggtg ttctgcacat
    10921 gcaggcgcac ccggcattca ggatggcgct gcatgaagtg gccgatcagc aaggtcgcca
    10981 ggtagttgcc caccgtcagg gtggcgccga cctgcagcga gccgaagccg ctgtgtccct
    11041 gcagcaactg ctcgatctcg ccggcgcggt cgagcagggc gaccgccttg ggcagcagct
    11101 cctcgccgag ggcgctgagc gccaggcgct tgccgatgcg gtcgaacagc gggctgtcgt
    11161 agtgccgctc gagttccgcc agcgaggtgc tggctgccga ctgcgacagc gccagcgact
    11221 cggcggcacg ggtgacgctc tccaggcggg cgacggcgac gaagacttcc agctgtctga
    11281 gagtaaagcg catatctata taaccgatta tctatatttt aattattcat ttagtggatg
    11341 ttttgtgcgg gtatagaata ttgctcatat agagccatcc ggcctcttcc tcgtttcagc
    11401 ttcccgcaag gagttatctc gtgagcaacc tgatgagtga gcgcgtagtc agcgtacacc
    11461 actggaacga caccctgttc agcttcaaga ccacccgtaa ccaaggcctg cgctttgaga
    11521 acggccagtt cgtgatgatc ggtctggaag cgaacggtcg tccgctcatg cgagcctaca
    11581 gcattgccag ccccaactac gaagagcatc tcgagttctt cagcatcaag gtgccggacg
    11641 gcccgctgac ctcgcggctg cagcacctgc agccgggcga cgagctggtg gtcagccgca
    11701 agcccaccgg cacgctggtt ctcggcgatc tgctgccggg caagcacctg tatctgctga
    11761 gcaccggtac cggcctggcg ccgttcatga gcgtgatcca ggaccccgag acctacgagc
    11821 gcttcgacaa ggtgatcctg gtccacggcg tgcgctgggt cagcgagctg gcctatgccg
    11881 acttcatcac caacgagctg ccgaacaacg agttcttcgg cgaggcggtc cgcgaaaagc
    11941 tgatctacta cccgaccgtg acccgcgagc cgttccgcaa ccaggggcgt cagaccgacc
    12001 tgatgcgcag cggcaagctg ttcagcgaca tcggcctgcc gccgatcaat ccgcaggacg
    12061 accgcgcgat gctctgcggc agcccgagca tgctcaagga aaccagcgaa gtactggaca
    12121 gcttcggcct caaggtctcc ccgcgcatgg gcgatccggg cgactacctg atcgagcgcg
    12181 ccttcgtcga gaagtaaggc cgggggcatc aaaaagcatc aagaaacggc ggagtcccgg
    12241 cagcgggccc cgccgttttg cattgtgcgg gggacgatgg gcagcggccg caggtcttgg
    12301 cccgcgacgc gggcggtgga acctcactgc gcgagtttcc cccttacggt tggtcgtgta
    12361 cgacttccag cacgcggatc cgccccggct ccgggtagtg ccagcgcacc tgcacgtccc
    12421 agaacaatac cccgtagcgc cgttccggcg tcggtacctg gtaggccggg cgcgggtcct
    12481 gggccaggca ctgttcgatc agctcgacta ccggctcggc caggcgcagc gcgtgctcgc
    12541 gggcctgctg cagggttgtg tcgctccatt cgaccgggat ggccggcggc ggtgcgtcgg
    12601 cgatggcgtt gtgtgcctcg ctgacgatat ccgcgtaggg cacgtagggc ttgatgtcga
    12661 gtatcggcgt gccgtccagc aggtcgatgc cggacaggtg caggcgtccc ggctcgaccc
    12721 gctccagctt gaccaccgac tggccgatgc cgttggggcg gtgggtggcg cggctggcga
    12781 acacgcccag cgagcggttg ccgcccaggc gcggcgggcg caccttgagg cgtggcgtgt
    12841 cctccagcgc ctggtggaac aggaacagca gccagacatg actgacctgc tccagcccgg
    12901 ccaccgcctc gccagtgtcg aacggcgcca ccagttccag cacgccgcgc gcggccggag
    12961 ccagctgcgg ttggcggggg atggcgaact tctccttgaa gcaggaacgc aggtaaccga
    13021 cggggctgac gctgtagggc atggcgggag tcttgcgcgg gcgggggagt ggcgatgata
    13081 cgcgccgggc agcgccagcg gcgagggctg caggggatgc ccaagcccct cttcccggca
    13141 gtcggaagag gggcgcgccg tgcttacagc ccgagcagct tggccacgta gtcggcatcc
    13201 ttgtcgccgc ggccggacag gttgaccagg atgtgctgat ccttcgccag cgtcggcgcc
    13261 acgcgcatgg cccaggccac cgcgtgggcg ctttccagcg ccgggatgat gccttcgacc
    13321 cgcgacaggg tcatgaacgc gtccaggcat tcctggtcgc tggcggtctg gtagctgacc
    13381 cggccgatgt ccttcaggta gctgtgctgc gggccgacgc cggggtagtc gaggccggag
    13441 gcgatcgagt gcaccgccgc cgggtggccc tcggcgtcct ccagcacgta gcaggccatg
    13501 ccgtgcagtt cgccgggctt gcccatcgac agggtggccg agtggcggcc gggcttgtcg
    13561 aggccttcgc cggccggctc gacgccgacc agcttgaccg cttcatcgtc gaggaaggcg
    13621 gtgaacatgc ccatggcgtt ggagccgccg ccgacgcagg cggtgacgtg gtcgggcagg
    13681 cggccgtgct tggcgaggaa ctgcgcgcgg gcctcggtgc cgatgatcga ctggaagtcg
    13741 cggaccatct tcgggaacgg atgcgggccg accaccgagc cgatggcgta gatgaagttg
    13801 tgcgggtcct tgaggtactc ctcgaaggcg ctgtccaccg cctccttgag ggtcgccgca
    13861 ccacgggtca ccggcaccag cgtgcagccg aggatcttca tcttggtgac gttggggtgt
    13921 tccttctcga tgtccacctg gcccatgtgg atctcgcagg ggatgcccac cagcgcgcag
    13981 gcggtggcca gcgccacgcc gtgctggccg gcgccggtct cggcgatcac cttgctcttg
    14041 cccatgaact tggccagcag cgcctcgccc aggcagtggt tgatcttgtg cgcgccggtg
    14101 tggttgagat cctcgcgctt gaggtggatc tgcgcaccgc cgagtttttc gctcaggcgg
    14161 cgggcatgga agatcgggct ggggcggccg acatagtcgg cgaacaggct ggccagctcc
    14221 tgctggaaat cctcgcgctg gcggatctcc tcgtaggcgg cgtcgatctc gtccatcacc
    14281 tgcttgaggt gcggcggcac cagctggccg ccgtaggagc cgaagtagcc gcgggcgtcg
    14341 ggcatggggg caaaacgatg ggaagcgctc atgcggtagg tactcatggg gaagggcgcg
    14401 cgacaggacg tcgccggccg caaatacatg agcatattac cgacgtggcg gtggctgcaa
    14461 aactccgccg cgctcggcaa ccatcaccgg ccgtgccgca gcgagctcaa tagtttcgct
    14521 cgtcataccc cgggaaccag tcctgcctga ggctgacgcc gcgttgcttg agggcattgc
    14581 cgacgctttc caccatgggt tcgggaccgg atagatagac cggtagctga gtcgccagcg
    14641 gctcctcgtc cagtatccgc tcggcgctga tcagctcgcc gatcaggtag cggacgtcga
    14701 gctccgggtg ccgggcggcc agttggtcga gttcgtcgcg gaaggcgatc tgcgcgtcgc
    14761 ggctgaagta cagcaggatg gcgttcagcg gcttgccgcg ggcgtcgcgt tccatgagca
    14821 tgctgcgaaa cggcgtcacg ccgatgccgc cggcgaccag caccaccggg gtgtccgact
    14881 cctcctccca ggtgaagtcg ccgcccaggt cgcgtacgcc gatgctgtcg cccggttgca
    14941 ggcggtcgag ggcctgcttg aaggcgctgg ggccgacgcg ggtggacacc tgcacgacct
    15001 gctcgctggg cgccgaggcg agggtgaacc agcgcaggtt gtcggcctcg tcggcgccgg
    15061 cctgcggcag gacgaaggcc tggttctggc cggcggtcca ggtcaggccg ccggtttcga
    15121 agacgaagct cttgatgttg ccggcttcgt tgcgtgacga gatcagtttg agctgttgca
    15181 tgtcggcgtc ctccgtttgc ccagcatagc agccggccag gacgttgccg gggcagggcc
    15241 ggcgtgctca ctgggcggtc agcagctgcc cgatcatgcc cagcagaaag ccggccaccg
    15301 cgcccagcgg tggcgcccag tggcgctgca gggggacttg cggagcgatg tcctggaaca
    15361 ccgagtagag gatgccgccg gaggcgaaca gcatgatggc gccgaccact tgcggatatg
    15421 gcgccagcca caggtagccg cagacgccgg ccagagggcc gagcagggcg atcagggtga
    15481 acaggccgat gatgcgccag ttggcgaggt gcgaagagcg atgcagttcg cggtaggcat
    15541 tgaagccttc cggcaggttc tgcagggcca tcagcagggc gagcagcagg ccggccgggc
    15601 tgccgagggc gactgtcgcg ccgagggcaa gggtttccgg gaggtagtcg aacagcatgt
    15661 cgatcgcctg gctggcggtg gtgcggtggg cggcgaggag acgatcgatc tgcatgaacg
    15721 ccacgccgcc agcggcaaag cacagggtcg tcagccacac cggcagctga cgcatgccct
    15781 cgggaaccag caccagggcc acggcggcga gcagcgcgcc ggcgccgaag gccagcacgg
    15841 catggcgcag ctcggtttcc agccagcgcg ggtggacatg ctcgcgactg gcgatcagcg
    15901 cgccaagcgg catggccagg ccgggcagca gggtgatgcc gagtatgtgc agccagacgt
    15961 tcattgcgag catccccatc cgtagaaagc ggctccatta cagcctatcc ggcagcgtta
    16021 gcccagagac gacgaacccg gcacggggcc gggttcgtct gtactgcgca gcgggtcggg
    16081 tcagatgact tcggcccaga ggtcgtactc gtcggcgtcg gtgacgcgca cgcgcacctt
    16141 gtcgcccggc ttgagcggct gctcgctgtc gacgtagacg ttgccgtcga tctccggggc
    16201 gtcggcggcg gagcggccga tggcgccctc ctcatcgact tcgtcgatca gcacctcgag
    16261 ctccttgccg atcttcagct gcaggcgcgc ggcgctgatc gcctgctggt gggccatgaa
    16321 gcgctcccag cgctcctgct tgatctcgtc cgccaccggc tcgaggccga ggtcgttggc
    16381 cggggcaccc tcgaccggcg agtactggaa gcagccgacg cgatccagct gggcctcggt
    16441 cagccagtcg agcaggtact ggaagtcctc ctcggtctcg ccggggaagc cgacgatgaa
    16501 ggtcgagcgg atggtcagtt ccgggcagat ctcgcgccac tgcttgatgc gcgccagggt
    16561 cttgtcctcg aaggccggac gcttcatggc cttgagcacc ttcggactgg cgtgctggaa
    16621 ggggatgtcc aggtacggca ggatcttgcc ggcggccatc agcgggatca cgtcgtcgac
    16681 gttggggtac gggtagacgt agtgcaggcg cacccacacg cccagcttgc cgagttcctc
    16741 gcacagctcg agcatgcgcg tcttcaccgg ctggccgttc cagaagtcca gcttgtactt
    16801 gaggtcgacg ccgtaggcgc tggtgtcctg ggagatcacc agcagttcct tgaccccggc
    16861 cttgaccagg cgctcggcct cgctgagcac ctcgccgacc gggcggctga ccagcttgcc
    16921 gcgcatcgag gggatgatgc agaaggcaca gctgtggttg cagccttcgg aaatcttcag
    16981 gtaggcgtag tggcgcgggg tcagcttgat gccctgcggc gggaccagat cgacgaaggg
    17041 atcgtggctg gtgctcagcg gcaccacctc gtgcacggcg ttgaccacct gctcgtactg
    17101 ctgcgggccg gtgaccgcca gcacgctggg gtgcacgttg cggatgctgt cctcggacac
    17161 gcccatgcag ccggtgacga tcaccttgcc gttctcggcc agtgcctcgc cgatggcgtc
    17221 caacgactcg gccttggcgc tgtcgatgaa gccgcaggtg ttgaccacca ccacgtcggc
    17281 atcctcatag gaggggacga tctggtagcc ctccatgcgc agctgggtga ggatgcgttc
    17341 ggagtcgacg gtcgccttcg ggcaaccgag ggaaacaaag ccgactttgg gcgtggcggt
    17401 ggacatgcag gctaacctcg aagggcgccg ggttggcgcc tctgatcaaa aagtgcgcaa
    17461 ttctagcgat gccggccgcg cttgaccagt gccgcgccgg caaacaaaag ccccgcgcgc
    17521 ggagcgggcg gggctggcga gcggcaggcg cggttcagcc gcgggccggc agcatgcgcg
    17581 agagggtgtt gtcgcggatg acgtaatggt gcagcaggcc ggcgaccgca tgcaggccga
    17641 tcagccagta gccggcgatg ccgccgaact cgtgcaggcc ttcgaggctt tccgccaggc
    17701 cgtgatcctt ctgcaccagc atcggcagct cgaagccgaa gaacggtgcc ggcttgcctt
    17761 cggcattgag gatcagccag ccgaccagag gcatgccaat catcagcacg tacagagcca
    17821 ggtgcatcag gtgcgacagg ccggtctgcc aggacggcgg cgccggctgg atcgccgggg
    17881 tcttgccgat caggcggccg agcagacgca gccagactag ggcgaagatg gccaggccga
    17941 gcatgaagtg ccagtgcttg agtccttcac ggatctcgct gccgcggacg aagttgccct
    18001 tgagttcgat gcaggcgtag accgcggcga tcagcagcag catgacccag tgcagggcaa
    18061 tattaatggg gctgtagcgg gaagaggcgg tgctcatgca gtgctcctgg cgcatccgag
    18121 taaagtcgat ggccggcagt ctagggcagc aagcttaagc caagctgagg aaggcgcgcg
    18181 ttgggctttc ttcgcgacaa atgaaaaagg ccattccgaa gaatggcctt ttcaaatttg
    18241 gttgcggggg caggatttga acctacgacc ttcgggttat gagcccgacg agctaccaga
    18301 ctgctccacc ccgcgtctgt gggcgccatt ctacgcaaaa gtgccggggt gtcaagcgcg
    18361 ggctcgaaaa aacctcaagt ggatcagggg gttagtgtcg agcccgcggg ggctggcgtc
    18421 ctgcagggca cgccgagcgg gggctgcagc gcaatggtgg tcccctggcc gggccggcga
    18481 gcggggctgc tccgccaggg ggcgcgccgg gtagtacact gcgcctctgt cgtttatttc
    18541 ccggagcctg ccgcctggcg gtgtgtcgtg cgcaagatca tccatattga ctgcgactgc
    18601 ttcttcgccg ccatcgagat gcgcgacgac ccgcggctgg ccgggcgtcc catggccgtg
    18661 ggcggtgcgc cggaccggcg cggagtgatc gccacctgca actacgaggc acgcgccttc
    18721 ggcgtgcgct cggccttgtc ctcgcggcag gcgctggcgc tgtgcccgca gttgctgatc
    18781 gtgcggccgc gcttcgaggc gtaccgcgag gcgtcgctgc agatccgcag catcttgcag
    18841 gactacaccg agctgatcga gccgctgtcg ctggacgaag cctatctgga cgtgtccgac
    18901 tcggcgcact ttgccggcag cgccacgcgc atcgcccagg acatccgccg gcgggtgcgc
    18961 gagcagctgg agatcacggt gtcggccggg gtggcaccga acaagttcct cgccaaggtg
    19021 gccagcgact ggcacaagcc ggatggcctg tgcgtgatca ccccggccga ggtggacgaa
    19081 ttcgtcgcgg tgttgccggt ggcgcgcctg cacggggtgg gcaaggtcac cgccgagcgc
    19141 ctggcgcgcc tcggcatcca gcgctgtggc gacctgcgcg agtggcagcg gctggcgctg
    19201 gtgcgcgagt tcggcagttt cggcgagcgg ctgtggcagc tggcgcgcgg catcgacgag
    19261 cggccggtgc aggtcgaccg gcgccgccag tcggtcagcg tcgaacacac cttcgatcgc
    19321 gacctgccgg acctggccgc ctgcacggcc gagctgccgg ccttgctgga gcagctggag
    19381 cgccgcttga cgcggctgga cagcggctac cgggtgggca agccgctggt caagctgaag
    19441 ttccacgact tcacccagac caccctggag caggccggcg tcagccgcga tgcggacagc
    19501 taccggcgcc tgctggcggc cgccttcacc cgcggtggca agccggtgcg cctgatcggc
    19561 gtcggcgtgc gcctgctcga cctgcgcgag ggcggcgaac agctcgagct gttctagctc
    19621 agccctcttc cggctgcagg cggatcggct tgccctgttc cggccacagg cgcagttgct
    19681 ccagtggcga caggtcccag cggcgcacat gctcgagcgc ctggaagaag cgggcttcct
    19741 gctccatcag cgccggcgca caggccttgc gcgtgctgcc gatctggctg aaacgcagga
    19801 tctcgccttc cacgccgtag ccggcgaacc agtggttgca gccgccggtg ccgtaggcac
    19861 ggtcgtcggc gccgaaggtg atggtcaggt ggctgccctc gagcagcggg cgctcgtcga
    19921 tccattcgac gcggtaggtg cgttcctgct ccagggccag cggggcgctg gcgcaaccgg
    19981 tcagggcgag ggcgaggacg gtcgggcggg tgaccagggc ggtacggtgc aacatcaggc
    20041 ctccttggcg atgcagcgcg ggcaaagatg gtggccggcc tggtggcgcc agcccagctc
    20101 ggtgatgcgt gcttcggcgg ccggggtgcg ggcggcggcg ccgagcttgg cgtcgacggc
    20161 aaactcgaag tccaggtgcg cgccacagcc gttgcagtcg accccccact gcagaatgct
    20221 cagttcgttg aacaccggcc ccttggccac cgccagccac tggccgggcg ggttgatcag
    20281 gtggcggacc ttctccacca ccaggcgcat ggacaggtcg cggctgccct tgagggtgac
    20341 cagcaggccg tcgccggcct ggatcgagcc gccgttgccg gtgacctgat agcggccggg
    20401 cgccagcgca cggcattcgg tgagggtgtg ttgcggattg agcaggttgt agcggaaatc
    20461 gtgttcagtc atggagcctc cgggcagtgt gctcatgcta gcacgctgcc tattacgctt
    20521 caggcctgca ctcggttggc ggcgcgcccg gcctgccagg cggcgatgtt gtccagggtg
    20581 gtggtggcga tcgcggccag cgcttcgcgg gtgaggaagg cctggtgcgc ggtgacgatc
    20641 acgttgggga aggtgagcag gcgcgccagc acatcgtcct gcagcggcag gtcggagcgg
    20701 tcctcgaaga acaggtcggc ctcctcctcg tagacatcca ggccgagata gccgagctgg
    20761 ccgctcttca gcgcgtcgat cagcgccggc gtatcgacca gcgcgccgcg gccggtgttg
    20821 atcagcatgg cgccgcgctt catccgctgc aggctggcgc cgtcaatcag gtggcgggtg
    20881 gccgggttca gcgggcagtg caggctgatg atgtcgctgc tggccagcaa ctcgtcgagc
    20941 gcgacgaagc gggcgccgag ggcctccagg gcggcatcgg gctgcggatc gtggagccgc
    21001 acccggcagc cgaagccgag catgatgcgg gcgaacaccg cgccgatctg gccgcaaccg
    21061 accacgccga cgcacttgcc gtgcaggtcg aagccggtca gcccgtgcag ggagaagtcg
    21121 ccttcgcggg tgcggttgaa ggcgcggtgc aggcgccggt tgagcgccag caccagggcc
    21181 acggcatgct cggccaccgc gtgcggcgag taggctggca cgcgcaccac cggcaggtcg
    21241 agggcctggg cggcgctcag gtcgacgtgg ttgtagccgg ccgagcgcag ggcgagcagg
    21301 cgggtgccgc cggcgcgcag gcgctcgagt accggcgcgg acaggtcgtc gttgacgaag
    21361 gcgcagacca ccgcgctgcc cgcggcgagg gcggcggtgt cggcatccag gcgcgcgtcg
    21421 agaaagtgca gctcatgacc gtggggctgg ttggccgtgg cgaagctgtc gcggtcgtag
    21481 ggcttgctgc tgaacagggt gatgcgcatg caatctccgg tcattacgtc ggcaggggga
    21541 ccggccgggc ggcctgttca gccaggcggg tgatggcggc gtccagctcg tcgagggcga
    21601 cgttggccgg ggcgttctgc ttgagcaggc tttcgctgcg ctggcaggcg gcgcgcagct
    21661 gcggcacccc gcagtagcgg gtggcgccat gcaggcgatg gatgcgttcg agcagcgcgg
    21721 tgctgtcgtt gctggcgcgg gcctggcgga tcacggcctg gtcgctgggc agcgagtcga
    21781 gcagcatgct cagcatgtcg gcggcgaggt cggccttgcc ggcggacagg cgcaggcctt
    21841 cggcggggtc gaggatcggc agttcggtgg ccgcgctgcc gcgctcgggc agcagcggct
    21901 cgcgcacttc gccgttgtgc agcgccaggc cggtccattt cagcaccacc tggctcagct
    21961 ggcgttcgct gatcggcttg gtcaggtagt cgtccatgcc gcactgcaac agggcgcgct
    22021 tctcgttggc catggcgtgg gccgtcagcg cgacgatcgg cagcggcggc tggccgtgct
    22081 cgctttccca ctggcggatt gcctcggtgg tttcgcggcc gttcatgccg ggcatctgca
    22141 cgtccatgaa caccaggtcg aaggtctcct gctgtacggc gcgcagggcg tcgtagccgt
    22201 tgtcgacggc cttgacctgg gcgcccatgt cgccgagcag ggtctgcacc agcatcaggt
    22261 tggccgggtt gtcgtcgacg cacagcaggc gcggcgcgcg gctggcggca gggttggtgg
    22321 cgccgctggc ttcgacccgc tgcacgcgcg ggctgagcag gtcgatcagg gtgcgctgca
    22381 gcttgctggt gcacgccggc ttggcctgca gctggccgtg gctctccggc aggctggcgt
    22441 ggtacagcgc ctgttcggtg gtcgggcaca gcagcagcgc ctggcagccc tgctggttga
    22501 gttcccacag ctggcggctc aggcgttccg gcgggtgttc gcgggcattg atgccgatca
    22561 gggcgatggc cagcggcgcg ctgccgtgct ggcgcagggc tgcttcctcg atcagtgcgt
    22621 cgagattggc gaactcgatc acctgcaggc cgcagtcctc cagctggtgg cgcagcgact
    22681 ggcgggccag ctggtggctt tccagcagcg cggcgcagcg gccaccgagc agcgcgcgcg
    22741 gcagttcgtc gcgctcctcg gcggccttgg gcagggtgag ctgcagccag aactcggtgc
    22801 cttcgtcctt ctcgctggtc acgccgatct cgccgcccat ctgctcgatc aggcgcttgg
    22861 agatcaccag gccgaggccg gtaccgccgg cctggcgcga gctggagttg tcgacctggt
    22921 tgaaggcctg gaacagcgag cgctgctcgc tctcggacag gccgacgccg ctgtcctgca
    22981 cgctgatgcg cagctgggcg tgactgacca ggtcttcctc gagcatggcg cgcaccacga
    23041 tgctgccact gtgggtgaac ttgatcgcgt tgctcaccag gttggtcagc acctgcttga
    23101 ggcgcagcgc gtcaccgacc agcgcctgcg gggtgtcgcg gtagatcagg cagaccagct
    23161 cgaggttctt ctcgtgggcg gcgggggcga ggatggtcag ggtgtcctgg atcaggtcgc
    23221 gcaggttgaa cggcgcctgc tcgagcacca gcttgccggc ctcgatcttg gagaagtcga
    23281 ggacctcgtt gatgatcgcc agcaggttgt cggccgactt ctggatggtc agcaggtagt
    23341 cctgctgacg gctgtcgagg ccggtcttgt gcagcaggtt ggtgaagccg aggatgccgt
    23401 tgagcggggt gcggatctcg tggctcatgt tggcgaggaa ctcggacttg atccggctgg
    23461 cctccagggc ctccttgcgc gccatggtca gctcgatgtt ctgcacctcg atggtttcca
    23521 tggtgttgcg caggtcttcg gtggcctgtt cgacgttgtg ctgcagctcc tcctgggcac
    23581 tttgcagcga ctcggccatg cggttgatgc cgctggccac ctcgtccagt tcatggctgc
    23641 cgagcgccgg caggcgggtc tgcagctggc cgtcgcggat ctgcgcgacg ccgtggctga
    23701 tctgttgcag tggccggctg atcgtgcggc tcatgcgcaa ggcgagcagg gcggtgacga
    23761 tcagtccggc gacggtcagc agcaggctgg tcagcaggct gcggtagccc tgcagcaaag
    23821 tgccctggtg ggacagctcc agctcgatcc agccgagcag gcggccatcc tcctcgacgg
    23881 gcgcatggcc gccgagctgg cggtgtaggc cgtagaccgg cagcaggaag cgcgtggcct
    23941 cggcactgct gaccaggctg agttgctgag gatcgcgcgg cggtgcgctg gtgcgcatgc
    24001 gtggacccgc atgcaccagg cgctcgcctt gcggggagag cagggtgatg gcgcgcaggt
    24061 cgggctgctc gaggatctgg cccgccaggc gttcgagcag ggggatatcg cgcgccacca
    24121 gcaggcgcgc actcagcggc gccagctgct cgatcaccag ttggccgcgc tcgacgagct
    24181 ggctgtgcat ctgcgacaga tgggtccagg tggagtagct accgagcacc agcgccaaca
    24241 ggctggtggg cagcagggac agcagcagga cgcggccctt gatcccgagt ttgtcgaaca
    24301 cgcagcgcct ccgcggcaag gggatggcgg cagtgtagcg tcttgccggg ggcgaacaag
    24361 ggtgcagttc acgggcggcg gtcgaggcga cgaggcgtcc gccgcgctgt gcttcttatt
    24421 atagctacaa ggaatatcga ccagccttgc cgtgttatcg gcccggcgcg tttgccgcta
    24481 tcatgggcgc cccctgtccg cgctgcgaag agctggctcc catgaccgtg cagtatccca
    24541 ccatcgccga ctgcgtcggc aacaccccgc tggtgcgcct gcagcgcctg ccgggcaaga
    24601 cctcgaacac ccttctggtc aagctggaag gcaacaaccc ggccggctcg gtgaaggatc
    24661 gcccggcgct gtcgatgatt ggccatgccg agctgcgcgg cgacatccag cccggcgaca
    24721 cgctgatcga ggccacttcc ggcaacaccg gcatcgccct ggccatggct gcggcgatca
    24781 agggctaccg gatgatcctg atcatgccgg acaacatgag cgccgagcgc aaggcggcga
    24841 tgagcgccta cggcgccgag ctgatccagg tgagcaaggc cgagggcatg gaaggcgccc
    24901 gcgatctggc gctgaagctg caggccgagg gccgcggcaa ggtgctcgac cagttcggca
    24961 accaggacaa tccgcaggcc cactacgaga gcaccggtcc ggaaatctgg cgccagaccg
    25021 gcgggaccat cacccacttc atcagctcga tgggcaccac cggcaccatc atgggcacct
    25081 cgcgctacct caaggagcag aacccggcgg tgcagatcgt cggcctgcag ccgcaggaag
    25141 gcgcggcgat tcccggcatc cgccgctggc cgcaggagta cctgccgaag atcttcgatg
    25201 ccagccgcgt cgaccgggtg atcgacatgg cgcagggcga ggccgaggac gtcatgcgcc
    25261 gcctggcgcg cgaggagggc atcttctgcg gcgtctcctc cggcggggcg gtggcggcga
    25321 tgctgcgcct gtcgcaggaa gtggagaacg cggtgatggt ggcgatcatc tgtgatcgcg
    25381 gcgaccgcta cctgtccacc ggggtctacg acttcccgca gggctgagcg tgacggcgca
    25441 gccgcaaggc tgcgcaacga cttccttccg gtgcgcctgg cgcaccctac actgaatctg
    25501 caggtcgcct gatggccaaa cgcaacacag ggctgcgctt ccagccgagc ggcggtgtac
    25561 gcaccgcgca actgccggta ggcaagaagc agctcctctc catcgagcgc ctggcccacg
    25621 acggtcgcgg tatcgcccac gaaggcgggc gcacctggtt cgtcgccggc gcgctggctg
    25681 gcgagcaggt cgaggcccgg gtgctggcgg cgcgcagcca ggtggtggag gc
//
LOCUS       DATCUN010000072        12903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_228257, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000072 DATCUN010000000
VERSION     DATCUN010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 12903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12903
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228257"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1791)
                     /gene="dnaK"
                     /locus_tag="VIO83_02905"
     CDS             complement(<1..1791)
                     /gene="dnaK"
                     /locus_tag="VIO83_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY8330263.1"
                     /translation="MGKIIGIDLGTTNSCVSVLENGVAKVIENAEGGRTTPSIIAYTN
                     DGEILVGQSAKRQAVTNPHNTLYAVKRLIGRRFEEDVVQKDIKLVPYKIAKADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAYGMDKAKGDHTIIVYDLGGGTFDVSVIEIAEVDGEHQFEV
                     LATNGDTFLGGKDFDIRLIDYLVDEFKKETGMDIKGDPLAMQRLKEAAEKAKIELSSS
                     QQTDVNLPYITADATGPKHLNVKISRAKLEALVEDLVARTIEPCRVALKDAGIDASKI
                     DDVILVGGQTRMPLVQQKVTEFFGKEARKDVNPDEAVAMGAAIQGAVLAGDVKDVLLL
                     DVSPLTLGIETLGGVMTALIEKNTTIPTKKSQVFSTADDNQGAVTIHVLQGERKQAGQ
                     NKSLGRFDLADIPPAPRGVPQIEVTFDIDANGIVNVSAKDKGTGKEQQIRIQASGGLS
                     DNDIEQMVRDAEQFAEEDKKRRAGAEAKNNAESLIHTTERQLQEHGDKVDAGLKSEIE
                     GAVAEAKAAVEGGDADAMNQKAQALAQVAMK"
     gene            complement(1913..2452)
                     /gene="grpE"
                     /locus_tag="VIO83_02910"
     CDS             complement(1913..2452)
                     /gene="grpE"
                     /locus_tag="VIO83_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY8330264.1"
                     /translation="MADEHKLDPQNPEAELQGEAETAGDDLAQRVQVLEEQLAAAQDQ
                     ALRAAAELHNVRRRAEQDVEKAHKFALEKFAADLLMVVDSLERGLELSDPADEAIRPM
                     REGMELTLKAFHDTLKRYQVEALDPHGEPFNPEQHQAMAMQPSASVEPGSVLKVFQKG
                     YLLNGRLLRPAMVVVSKAE"
     gene            2600..4285
                     /gene="recN"
                     /locus_tag="VIO83_02915"
     CDS             2600..4285
                     /gene="recN"
                     /locus_tag="VIO83_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003148709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY8330265.1"
                     /translation="MLVHLSIRNYAIVEQLDLELRAGMSVITGETGAGKSIMLDALGL
                     ALGDRTDSGVVRPGADKADILASFDLGSIAEAQHWLAERDLDNDGPCILRRVITAEGR
                     SRAYINGTPCPLADLKHLGELLIDIHSQHEHQSLLKPDTHRRLLDAYAGGGELARQVQ
                     LAAQRWRQTRSELERLSRSSEEQHARQQLLSYQLEELDNLALGSHELEQLEQEHKTLG
                     NAESLLGACRQVLELCSESDSGNVLGALTSSLQRLNGGPGQPAALEEAVNLLASAQIQ
                     VEEAVSSLNRFLDHFEADPQRLQQIEERLDSIYSLARKHRILPTELHALQEQLRGELQ
                     ALHADDAAVERLGAELEAYARHYREKAGELSALRREAAGRLAAAVETEMQRLGMPGGR
                     FAIALQPSDSAELQPQGQEQVEFQVSANPGQPLKGLARVASGGELSRISLAIQVITAQ
                     TSRVPTLVFDEVDVGIGGPTAEVVGQLLRRLGERGQVITVTHLPQVAAQGHQHLFVHK
                     ERGSSETRTAVAQLGHNQRIEEVARMLGGLDLTRESLAHARKMVQGAQRAAAA"
     gene            complement(4359..4763)
                     /gene="fur"
                     /locus_tag="VIO83_02920"
     CDS             complement(4359..4763)
                     /gene="fur"
                     /locus_tag="VIO83_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEY8330266.1"
                     /translation="MVENNELRKVGLKVTLPRVKILQMLDSAGQRHMSAEDVYKALME
                     AGEDVGLATVYRVLTQFEAAGLVERHNFDGGHAVFELSDGAHHDHMINVDTGEVVEFI
                     DADIEKLQREIAAQHGFDLVDHKMVLYVSKRK"
     gene            4860..5438
                     /locus_tag="VIO83_02925"
     CDS             4860..5438
                     /locus_tag="VIO83_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEY8330267.1"
                     /translation="MQKAKLMLTGLTFLGLAALAGCSLPGVYKIDIQQGNVITQDMID
                     QLRPGMTRRQVRFIMGNPLITDTFHANRWDYLYSLQPGGGTRQQERISLLFDSNDQLT
                     GLAGDFMPGVSRDEAIRGDSGSTAVTEAPTTEEGASTEEAVPAQAEPEAPPAPGSLLE
                     QLQREVDEARPVAVPTPAPLEVEPTPEAEPAE"
     gene            complement(5506..5829)
                     /locus_tag="VIO83_02930"
     CDS             complement(5506..5829)
                     /locus_tag="VIO83_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEY8330268.1"
                     /translation="MDKPDTIAVEVVYALADKQKLLRLSVPYGTSMREAAERSGIAGL
                     FPGLDLATAPMGIFGKAVARPEERVLEEGERVEIYRPLIADPKEARKQRAAKVAKVKA
                     ESGDE"
     gene            complement(5822..6256)
                     /locus_tag="VIO83_02935"
     CDS             complement(5822..6256)
                     /locus_tag="VIO83_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEY8330269.1"
                     /translation="MSTRIQRSALLPYPAQALYDLVNDVASYPQFLPWCSAAQVLESS
                     PAKMRATLKVGKAGISQQFTTCNTLVPGESIEMTLEQGPFRALHGVWSFQALNEKACK
                     ITLDLSFDYSGALVKATLGPLFNQAAATMVDAFCQRAKQLYG"
     gene            6367..6849
                     /gene="smpB"
                     /locus_tag="VIO83_02940"
     CDS             6367..6849
                     /gene="smpB"
                     /locus_tag="VIO83_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY8330270.1"
                     /translation="MANQKKHPAGTIALNKKALHDYYIEQRFEAGIALAGWEIKSLRA
                     GKGQLTDSYVLLKDGEAWLLGSHITPLNAASTHVIADPTRTRKLLLHGKELDKLHGAV
                     QQKGFACVALALYWKKHLVKCEIALGKGKKEYDKRAVEKERDSNREIQRAVRSKGKDE
                     "
     gene            complement(6898..7674)
                     /locus_tag="VIO83_02945"
     CDS             complement(6898..7674)
                     /locus_tag="VIO83_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8330271.1"
                     /translation="MGFGPVRQRRLSDDIVERLEAMILEGSLKAGERLPAERVLAEQF
                     GVSRPSLREAIQKLAARGLLSSRQGGGTFVAEGLGSAFSDPLLQLLEGSEEGQRDLLE
                     FRHTLEGACAFYAAQRATPVDHLRLREAFERLQQCYVASVPGGGAEEGLADASFHLAI
                     AEASHNAVLLHTIKGLFDLLKRNVVTNIGGMYAQRGETRDMLMRQHRELFEAIVAGRA
                     EDARAISSRHIDYVQEVLAEVQQEGRREARARRRQSVQEG"
     gene            7984..9678
                     /locus_tag="VIO83_02950"
     CDS             7984..9678
                     /locus_tag="VIO83_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEY8330272.1"
                     /translation="MQPGILALLAFSPILLAAILLVGLRWPAKRAMPLVYLLTAGIGL
                     YAWDMSLNRVLASTVQGLIITVGVLWIIFGAILLLNTLKHSGGITAIRAGFAVISPDR
                     RIQAIIIAWLFGCFIEGASGFGTPAAIAAPLLVAIGFPALAAVMLGMLVQSTPVSFGA
                     VGTPIIIGVNSGLDTATLGARLVEQGSSWEVFLQLITSNVAIIHAIVGTVMPLIMVLM
                     LTRFFGKEKSWKAGFEVLPFAVFAGLAFTLPYAATGVFLGPEFPSLAGGLIGLAIVTT
                     AARFGFLMPKSTWDFADAKDWPSEWLGSVEMKLDQLTAKPMGTLRAWLPYVLVGLLLV
                     ISRVFPEVSAALKSVVLVFPDLLGEKGIKADFQPLYLPGGILVAVVIATFFLHGMKLR
                     DLGTAVGESSKVLLGAGFVLLFTVPMVRILINSGVNASELPSMPIAMAQWVADSVGGI
                     YPLLAPSVGALGAFIAGSNTVSNMMLSQFQFGVAESLGISGALIVAVQAVGAAAGNMV
                     AIHNVVAASATVGLLGREGTTLRKTVWPTFYYILFTGLIALIAIYVLGVSDPLVAG"
     gene            9746..10891
                     /gene="lldD"
                     /locus_tag="VIO83_02955"
     CDS             9746..10891
                     /gene="lldD"
                     /locus_tag="VIO83_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004457 - lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent L-lactate dehydrogenase LldD"
                     /protein_id="HEY8330273.1"
                     /translation="MIISASTDYRAAAQRRLPPFLFHYIDGGANAEYTLKRNVEDLAG
                     IALRQRVLKNMSELNLGTELFGEQLALPVALAPVGLTGMYARRGEVQAARAAASKGIP
                     FTLSTVSVCPIEEVAPAIDRPMWFQLYVLKDRGFMKNALERAKAAGVTTLVFTVDMPV
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPHWALDVGLLGKPHDLGNISAYRGNPTGLE
                     DYIGWLGNNFDPSISWKDLQWIRDFWDGPMVIKGILDPEDARDAVKFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGELKILVDSGIRTGLDVVRMLALGADSVMLGRAFVY
                     ALAADGEAGVSNLLELIDKEMRVAMTLTGAKSIAEISRDSLVRELGA"
     gene            10959..>12903
                     /locus_tag="VIO83_02960"
     CDS             10959..>12903
                     /locus_tag="VIO83_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEY8330274.1"
                     /translation="MSLPAAFLAAVERLIPSERRFDDPLSTLAFGTDASFYRLIPKLV
                     LRVESEREVVELLKLAHAQQVAVTFRAAGTSLSGQAISDSVLIVLGDHWNGRDIRAGG
                     AQIRLQPGVIGANANAVLAPFQRKIGPDPASINAAKIGGIVANNSSGMCCGTAQNSYQ
                     TLAGLRLVLADGTVVDSEDPASVAAFRQSHAALLEQLAQLGRETRANSALADKIRHKY
                     RLKNTTGFSLNALVDFDEPLDILNHLMVGSEGTLGFISAVTYDTVPDYPHKASALVVF
                     PDVENCCRAVPLLKQQPVSAVELLDRRSLRSVENMQGMPVWVRELSSGACALLIESRA
                     ASQSLLHEQIAWIMASIADFPVEKQVDFSEDPAVYNQLWKIRKDTFPAVGAVRQTGTT
                     VIIEDVTFPVERLAEGVNRLLELLDRHHYDEAILFGHALEGNLHFVFTQGFDDPAQVA
                     RYEAFMGEVAQLVAVEFGGALKAEHGTGRNMAPFVELEWGSEAYALMWQIKRLLDPKG
                     ILNPDVVLSEDPQIHLKHLKPLPAADEIVDKCIECGFCEPVCPSNGLTLTPRQRIVIW
                     RDIQAKKRAGIDTTELEQAYHYYGLDTCAATGLCAQRCPVGINTGDLVRKLRAAEAGH
                     AGSATWLARHFATAMQGTRLTLAA"
BASE COUNT         2096 a         4649 c         4043 g         2115 t
ORIGIN      
        1 cttcatcgcg acctgggcga gcgcctgcgc cttctggttc atcgcatcgg cgtcgccgcc
       61 ctcgaccgcg gccttggcct cggccaccgc gccctcgatc tcggacttga ggcccgcatc
      121 gaccttgtcg ccatgctcct ggagctggcg ctcggtggtg tggatcaggc tctcggcgtt
      181 gttcttcgcc tcggcccctg cgcggcgctt cttgtcctct tcggcgaact gctcggcgtc
      241 gcggaccatc tgctcgatgt cgttgtcgct gagcccaccc gacgcctgga tgcggatctg
      301 ctgctccttg ccggtgccct tgtccttggc gctgacgttg acgatgccgt tggcgtcgat
      361 gtcgaaggtg acctcgatct gcggcacgcc gcgcggcgcc ggcgggatgt cggccaggtc
      421 gaagcggccc agcgacttgt tctgcccggc ctgcttgcgc tcgccctgca gcacgtggat
      481 ggtcaccgcg ccctggttgt cgtcggcggt ggagaacacc tgcgacttct tggtcgggat
      541 ggtggtgttc ttctcgatca gcgcggtcat cacgccaccg agggtctcga tgcccagggt
      601 cagcggcgac acgtcgagca gcagcacgtc cttgacatcg ccggccagca cggcgccctg
      661 gatggcagcc cccatggcca cggcttcgtc cgggttgacg tccttgcgcg cttccttgcc
      721 gaagaactcg gtgaccttct gctgcaccag cggcatgcgg gtctggccgc cgaccaggat
      781 cacgtcgtcg atcttgctgg cgtcgatgcc ggcatccttc agcgccacgc ggcacggctc
      841 gatggtgcgc gcgaccagat cctcgaccag cgcttccagc ttggcgcggg agatcttcac
      901 gttgaggtgc ttcggaccgg tggcgtcggc agtgatgtac ggcaggttga cgtcggtctg
      961 ctggctggag gacagctcga tcttggcctt ctcggcggct tccttcaggc gctgcatggc
     1021 cagggggtcg cccttgatgt ccatgccggt ttctttcttg aactcgtcga caaggtagtc
     1081 gatcagacgg atgtcgaagt ccttgccacc gaggaaggtg tcgccgttgg tagccagcac
     1141 ttcgaactgg tgctcgccgt cgacctcggc aatctcgatc accgacacgt cgaaggtccc
     1201 gccgcccagg tcatagacga tgatggtgtg gtcgcccttg gccttgtcca tgccgtaggc
     1261 cagcgccgcc gcggtcggct cgttgatgat gcgcttgacg tccagaccgg cgatgcgacc
     1321 ggcgtccttg gtggcctggc gctggctgtc gttgaagtag gccggcacgg tgatcaccgc
     1381 ttcggtgacc ggttcgccga ggtagtcttc ggcggtcttc ttcatcttct tcagcacttc
     1441 ggcgctgatc tgcggcggcg ccatcttctg gcccttcact tccacccagg cgtcaccgtt
     1501 gtcggccttg gcgatcttgt agggcaccag cttgatgtcc ttctgcacca cgtcttcttc
     1561 gaagcgacga ccgatcaggc gcttcaccgc gtacagggtg ttgtgcgggt tggtcacggc
     1621 ctggcgcttg gcgctctggc cgaccaggat ctcgccatcg ttggtgtagg cgatgatcga
     1681 cggcgtggta cggccgcctt cggcgttctc gatgaccttg gccacgccgt tctccagcac
     1741 ggacacgcag gagttggtgg tgcccaggtc gataccgatg attttgccca tattactctc
     1801 ccgaaattct gactcggagc gacgcttctg gctgccgccc ggatgactgg atgcttggca
     1861 ctacagatgg gggtgccccg acgactttca agggcattcc cggcaattca cttcactcgg
     1921 ccttgctcac caccaccatg gcgggacgca gcaggcggcc gttgagcagg tagcccttct
     1981 ggaacacctt gagcacgctg cccggctcca cgctggcgct cggctgcatg gccatggcct
     2041 ggtgctgctc ggggttgaac ggctcgccgt gcggatcgag cgcctcgacc tggtagcgct
     2101 tgagggtgtc gtggaacgcc ttgagggtca gctccatgcc ttcgcgcatc ggacggatgg
     2161 cctcgtcggc cggatcggac agctcgaggc cgcgctccag actgtcgacc accatcagca
     2221 ggtcggcagc gaacttttcc agggcgaact tgtgcgcctt ctcgacgtcc tgctcggcac
     2281 gccgacgcac gttgtgcagc tcggccgcag cccgcagggc ctgatcctgg gcggccgcca
     2341 gctgctcctc gagcacctgc acgcgctgcg ccagatcgtc gccggcggtc tcagcctcac
     2401 cctgcagctc ggcttcgggg ttctgcggat caagcttgtg ttcgtcagcc atgggggtct
     2461 ccttgaaagc aacggtcggg cactttggcc cttgattcag ccgcctatat ggggggcctc
     2521 ggcgccggct tcaaggggga ttggcagaac gaaaaaatgt actgtataaa taaccagaca
     2581 acttcctcgg gagcccgcca tgctggtcca tctgtcgatc cgaaactacg ccatcgtcga
     2641 gcagctcgat ctggagctgc gcgccggcat gagcgtcatc accggcgaaa ccggcgccgg
     2701 caagtcgatc atgctcgacg ccctcggcct cgccctgggc gaccgcaccg acagcggcgt
     2761 ggtccgcccc ggtgcggaca aggccgacat cctcgccagc ttcgacctcg gcagcatcgc
     2821 cgaagcgcag cactggctgg ccgagcgcga cctcgacaac gacggcccgt gcatcctgcg
     2881 ccgagtgatc accgccgagg gccgctcacg cgcctacatc aacggcactc cctgcccgct
     2941 ggccgacctc aagcacctcg gcgagctgct gatcgacatc cacagccagc acgaacacca
     3001 gtcgctgctc aagcccgaca cccatcgccg cctgctcgac gcctacgccg gcggcggcga
     3061 actggcccgc caggtgcagc tggccgcgca gcgctggcgc cagacccgca gcgagctgga
     3121 gcgcctcagc cgcagcagcg aggagcagca cgcccgccag caactgctca gctaccagct
     3181 ggaagaactg gacaacctcg ccctcggcag ccacgaactg gagcagctgg aacaggagca
     3241 caagaccctg ggcaacgccg agagcctgct cggcgcctgc cgccaggtgc tcgaactgtg
     3301 cagcgagagc gacagcggca acgtgctcgg cgccctgacc agcagcctgc agcgcctgaa
     3361 cggcggcccc ggccagcccg ccgccctgga agaagcggtg aacctgctgg ccagcgcaca
     3421 gatccaggtc gaggaagcgg tcagcagcct caaccgcttc ctcgaccact tcgaagccga
     3481 cccgcagcgc ctgcagcaga tcgaggaacg cctggacagc atctacagcc tggcgcgcaa
     3541 gcaccgcatc ctgcccaccg agctgcacgc cctgcaggaa cagttgcgcg gcgaactgca
     3601 ggccctgcat gccgacgacg ccgccgtcga gcgcctcggc gcggaactgg aagcctacgc
     3661 ccgccattac cgggaaaagg ccggcgaact gagcgcactg cgccgcgagg ctgccggccg
     3721 cctggcggcc gcagtggaaa cggaaatgca gcgcctgggc atgcccggcg gacgcttcgc
     3781 catcgccctg cagccgagcg acagcgcgga gctgcaaccg cagggccagg aacaggttga
     3841 attccaggtc agcgccaacc ccggccagcc gctcaagggc ctggccaggg tcgcctccgg
     3901 cggcgagctg tcgcgcatca gcctggctat ccaggtgatc accgcgcaga cctcgcgcgt
     3961 gccgaccctg gtgttcgacg aagtggacgt cggcatcggc ggccccaccg ccgaggtggt
     4021 cggccagctg ctgcgtcgtc tcggcgaacg cggccaggtg atcaccgtca cccacctgcc
     4081 acaggtcgcc gcccagggcc accagcacct gttcgtgcac aaggagcgcg gcagcagcga
     4141 gacccgcacg gcggtggccc agctcggcca caaccagcgc atcgaggagg tggcgcgcat
     4201 gctcggcggc ctcgacctca cccgcgagtc actggcccac gcacgcaaga tggtgcaggg
     4261 cgcgcagcgc gctgcggccg cctgagtcga ccgctcccca ctgacccata aacgccaacg
     4321 gcgacccgca ggtcgccgtt ggcttgcctt cgccagcctt acttgcgctt gctgacgtac
     4381 agcaccatct tgtgatcgac cagatcgaag ccgtgctggg cggcaatctc gcgctgcagc
     4441 ttctcgatat cggcatcgat gaactcgacg acctcacccg tatcgacgtt gatcatgtga
     4501 tcgtggtgcg caccgtcgga cagctcgaag accgcatggc cgccatcgaa gttgtgccgc
     4561 tccaccaggc cggccgcctc gaactgggtc agcacccggt acacggtagc caggccgaca
     4621 tcctcgcccg cctccatcaa cgccttgtag acatcctcgg cactcatgtg ccgctgtccg
     4681 gcggagtcca gcatctgcag gatcttgacc cgtggcaggg tgaccttcag ccccaccttg
     4741 cgcaactcgt tgttctctac catgttcagc tttctcgcgg aggaatgctt cgcagcctcc
     4801 ctcaatgcag gtatgatccc ggttctgttg cccgccaaga tagtggaaga gatccaccga
     4861 tgcaaaaagc caagctcatg ctgaccggcc tcaccttcct gggactcgcc gcactcgccg
     4921 gctgttcact gccgggggtc tacaagatcg atatccagca gggcaacgtg attactcagg
     4981 acatgataga ccagttgcgc cccggaatga cccggcgcca ggtgcggttt atcatgggta
     5041 acccgctgat caccgacacc ttccacgcca atcgctggga ttacctgtac agcctccaac
     5101 cgggcggcgg tactcgccag caggagcgca tcagcctgct gttcgacagc aacgaccaac
     5161 tgaccggtct ggctggcgac ttcatgccgg gcgtgagccg cgacgaggcc atccgcggcg
     5221 acagcggcag caccgccgtc accgaggcac cgaccaccga ggaaggcgcc agtaccgaag
     5281 aagcagttcc agcccaggcc gaaccggaag ccccccctgc tccgggctcg ctgctggagc
     5341 aactgcagcg cgaagtcgac gaggcccggc ccgtcgcggt accgacgccg gcacctctgg
     5401 aagtcgaacc gactccggaa gccgaaccgg ccgaataagc gccaggacag caaaaagccc
     5461 gagcatagac tcgggctttt tcgttgccgc ggggaaaacc gcgcctcact cgtcgccgct
     5521 ttccgccttg accttcgcca ccttggccgc gcgctgcttg cgcgcctcct tggggtcggc
     5581 gatcagcggc cggtagattt ccacccgttc gccctcctcc agcacccgct cctcgggcct
     5641 ggccaccgcc ttgccgaaga tgcccatcgg cgccgtggcc agatccagcc ccgggaacaa
     5701 cccggcaatt cccgaacgct cggcggcctc gcgcatgctg gtgccgtagg gcacgctcag
     5761 gcgcaacagt ttctgcttgt cggcgagggc gtagaccacc tcgacggcga tggtgtccgg
     5821 cttatccata tagctgcttg gctctctggc agaaggcgtc gaccatggtg gcggcggcct
     5881 gattgaacag cggaccgagg gtcgccttga ccagcgcccc ggaatagtcg aaggacaggt
     5941 cgagggtgat cttgcaggcc ttctcgttca gcgcctggaa gctccacacg ccatgcaggg
     6001 cgcggaacgg cccctgctcc agggtcatct cgatcgactc gcccggcacc agggtgttgc
     6061 aggtggtgaa ctgctggctg atccccgcct tgcccacctt gagcgtcgcg cgcatcttgg
     6121 ccgggctgct ctccagcacc tgcgccgccg aacaccaggg caggaactgc gggtagctgg
     6181 ccacatcgtt gaccaggtca tacagcgcct gcgccggata gggcagcagg gccgagcgtt
     6241 ggatgcgcgt gctcatgcag ggactcgcca gaaaatgtcc acacattgtc cggaattcgc
     6301 cgcagcggct caagcacacc gacacgcatg gcgcggcgcc aggcaagtcc ctataatgcg
     6361 cagcctatgg ctaatcagaa aaaacacccc gccggcacca tcgcgctgaa caagaaggcg
     6421 ctgcacgact actacatcga gcagcgcttc gaggccggca tcgccctggc cggatgggaa
     6481 atcaagagtc tgcgcgccgg caagggccag ctcaccgaca gctacgtgct gctcaaggac
     6541 ggcgaggcct ggctgctcgg cagccacatc accccgctga acgcggccag cacccacgtc
     6601 atcgccgacc cgacgcgtac gcgcaagctg ctgctgcacg gcaaggagct ggacaagctg
     6661 cacggcgccg tccagcagaa gggcttcgcc tgcgtggccc tggcgctgta ctggaagaag
     6721 cacctggtca agtgcgagat cgccctgggc aagggcaaga aggaatacga caagcgcgcc
     6781 gtggagaagg agcgcgactc caaccgcgag atccagcgcg cggtacgcag caagggcaag
     6841 gacgagtaac tcgccagccc cttggcgccg gcactcgctg ccggcgcacc cctcgcctca
     6901 cccctcctgc acgctctgcc gccgccgcgc ccgcgcctcg cgccgcccct cctgctgcac
     6961 ctcggccagc acctcctgca cgtagtcgat gtgccggctg gaaatcgccc gcgcatcctc
     7021 ggcacgccct gcgacgatcg cctcgaacag ttcgcgatgc tggcgcatca gcatgtcgcg
     7081 ggtttcgccg cgctgcgcgt acatgccgcc gatgttggtc accacgttgc gcttgagcag
     7141 gtcgaacagc cccttgatgg tgtgcagcag caccgcattg tggctggcct cggcgatcgc
     7201 caggtggaag ctggcatcgg ccaggccctc ctccgcacca ccgcccggca ccgaggcgac
     7261 gtagcactgc tgcagacgct cgaaggcctc gcgcaggcgc aggtgatcca ccggcgtcgc
     7321 ccgctgcgcc gcgtagaagg cacaggcgcc ctccagggtg tgacgaaact ccagcaggtc
     7381 gcgctgaccc tcttcgctgc cctccagcag ttgcagcagc gggtcgctga acgccgagcc
     7441 caacccttcg gcgacaaagg ttccgccacc ctggcgactg ctcagcaggc cgcgcgccgc
     7501 cagcttctgg atcgcctcgc gcagtgacgg ccgcgacacg ccgaactgct cggccagcac
     7561 gcgctcggcc ggcaagcgct cgccggcctt gagcgagccc tcgaggatca tcgcctcgag
     7621 gcgctcgacg atgtcatccg acagacggcg ctgccgaacc ggaccaaatc ccattgcctt
     7681 gcctccactg gtttgaccac cacgaccctt gcccgggcgc accgcccgca agcctaacgc
     7741 cgccaccggg aaccttacag tgcagcacct gccgaccaaa gtcgtaggag cctggattga
     7801 cagctccacg gccgactttt accctgaccc acgccgattg taaattggtc ttaccaatta
     7861 acaccaacaa atccaataac cggccgacga ggtcccgcca tgcccgctcc gcttacccgt
     7921 cacacctgcc gtacccccct tgcctgctcg cgcgtcgcgc gccactgatc cgaggtcatc
     7981 gtcatgcaac ccggcattct cgcgctactc gcgttttccc cgatcctgct cgccgccatc
     8041 ctgctggtcg gcctgcgctg gcccgccaag cgcgccatgc ccctggtcta cctgctcacc
     8101 gccggcatcg gcctgtacgc ctgggacatg agcctcaacc gggtgctcgc ttccaccgtg
     8161 cagggcctga tcatcaccgt cggcgtgctg tggatcattt tcggcgccat cctgctgctc
     8221 aacaccctca agcactccgg cggcatcacc gcgatccgcg ccggcttcgc cgtgatcagc
     8281 cccgaccggc gcatccaggc gatcatcatc gcctggctgt tcggttgctt catcgagggc
     8341 gcctccggct tcggcacccc cgccgccatc gccgccccgc tgctggtcgc catcggcttc
     8401 ccggcgctgg ccgcggtgat gctcggcatg ctggtgcaga gcaccccggt atccttcggc
     8461 gcggtcggca cgccgatcat catcggcgtc aacagcggcc tggataccgc cacgctgggc
     8521 gcgcgcctgg tcgagcaggg ctccagctgg gaagtcttcc tgcagctgat caccagcaac
     8581 gtggcgatca tccacgccat cgtcggcacc gtgatgccgc tgatcatggt gctgatgctg
     8641 acccgcttct tcggcaagga gaaaagctgg aaggccggct tcgaggtgct gccgtttgcc
     8701 gtgttcgccg gcctggcctt caccctgccc tacgccgcca ccggcgtgtt cctcggcccc
     8761 gagttcccct cgctggccgg cggactgatt ggcctggcca ttgtcaccac cgccgcccgc
     8821 ttcggcttcc tgatgcccaa gagcacctgg gacttcgccg atgccaagga ctggccgagc
     8881 gagtggctgg gcagcgtgga aatgaagctc gaccagctga ccgccaagcc gatgggcacc
     8941 ctgcgcgcct ggctgcccta tgtgctggtc ggcctgctgc tggtgatcag ccgcgtgttc
     9001 ccggaagtca gcgccgcgct gaaatcggtg gtgctggtgt tccccgacct gcttggcgag
     9061 aaaggcatca aggcagactt ccagccgctg tacctgccgg gcggcatcct ggtcgccgtg
     9121 gtcatcgcca ccttcttcct gcacggcatg aagctgcgcg acctcggcac cgcggtcggc
     9181 gagtcgagca aggtgctgct cggcgccggc ttcgtgctgc tgttcaccgt gccgatggtg
     9241 cgcatcctga tcaactccgg ggtcaacgcc agcgagctgc cgagcatgcc gatcgccatg
     9301 gcgcagtggg tggccgacag cgtcggcggc atctacccgc tgctggcgcc gagcgtcggt
     9361 gccttgggcg ccttcatcgc cggctccaac acggtgagca acatgatgct cagccagttc
     9421 cagttcggcg tggcggaaag cctgggcatc tccggcgcgc tgatcgtcgc cgtgcaggcc
     9481 gtcggcgccg ccgccggcaa catggtggcg atccacaacg tggtggccgc ttccgccacc
     9541 gtcggcctgc ttggccgcga gggcaccacc ctgcgcaaga ccgtgtggcc gactttctac
     9601 tacatcctgt tcaccggcct gatcgccctg atcgcgatct acgtcctcgg cgtcagcgac
     9661 ccgctggtgg ccggctaagc caaagcccga ccccgccccg gcccgcccgg ggcggcgtcc
     9721 ctgcgaacct accgggaact gccccatgat catctccgcc tctaccgact accgcgccgc
     9781 cgcccagcgg cgcctgccgc cgttcctgtt ccactacatc gacggcggcg ccaatgccga
     9841 atacaccctc aagcgcaacg tcgaggatct ggccggtatc gccctgcgcc aacgggtgct
     9901 gaagaacatg tccgagctga atctcggcac cgagctgttc ggcgagcagt tggccctgcc
     9961 ggtggcgctg gcgccggtcg gcctgaccgg catgtacgcg cggcgcggcg aagtgcaggc
    10021 ggcacgtgcc gcggcgtcca agggcattcc cttcaccctc tccaccgtgt cggtctgccc
    10081 gatcgaggaa gtggcgccgg ccatcgaccg gccgatgtgg ttccagctct acgtgctcaa
    10141 ggaccgcggc ttcatgaaga acgccctgga gcgcgccaag gccgccgggg tcaccaccct
    10201 ggtgttcacc gtcgacatgc cggtgcccgg cgcccgctac cgcgatgccc actccggcat
    10261 gagcggcccc aacgccgcca tgcgccgcta cctgcaggcg gtcacccacc cgcactgggc
    10321 actggacgtc ggcctgctcg gcaagccgca cgacctgggc aacatctccg cctatcgcgg
    10381 caaccccacc ggcctcgagg actacatcgg ctggctgggc aacaacttcg acccgtcgat
    10441 ctcctggaag gatctgcagt ggatccgcga cttctgggac ggcccgatgg tgatcaaggg
    10501 tatcctcgac ccggaagacg cccgcgacgc ggtcaagttc ggcgccgacg gcatcgtcgt
    10561 ctccaaccac ggcggccgcc agctcgacgg cgtgctgtcc agcgcccgcg ccctgccggc
    10621 gatcgccgac gcggtgaagg gcgagctgaa gatcctcgtc gactccggca tccgcaccgg
    10681 cctcgacgtg gtgcgcatgc tcgccctcgg cgccgacagc gtgatgctcg gccgcgcctt
    10741 cgtctacgcc ttggccgccg acggcgaggc cggggtgagc aacctgctgg agttgatcga
    10801 caaggaaatg cgcgtggcca tgaccctgac cggcgccaaa tcgatcgcag agatcagccg
    10861 cgactcgctg gtccgcgaac tgggcgcctg acgccaccgc ctccaccgca ggagccagcc
    10921 agctggcgcc tgcaagtacc gtgattgccg gagtccccat gagcctgccc gccgccttcc
    10981 ttgccgccgt cgaacgcctg atcccgagcg agcggcgctt cgacgacccg ctgtccaccc
    11041 tggccttcgg caccgacgcc agcttctacc ggctgatccc caagctggtg ctgcgcgtcg
    11101 agtccgagcg cgaggtggtc gagctgctca agctggccca cgcccagcag gtggcggtga
    11161 ccttccgcgc cgccggcacc agcctgtccg gccaggcgat cagcgactcg gtgctgatcg
    11221 tcctcggcga ccactggaac ggccgcgaca tccgcgccgg cggcgcgcag atccgcctgc
    11281 agccgggggt gatcggcgcc aacgccaacg ccgtgctggc gccgttccag cgcaagatcg
    11341 gcccggaccc ggcgtcgatc aacgcggcga agatcggcgg catcgtcgcc aacaactcca
    11401 gcggcatgtg ctgcggcacc gcgcagaaca gctaccagac cctcgccggc ctgcgcctgg
    11461 tgctggccga cggcacggtg gtggacagcg aagacccggc cagcgtcgcc gccttccggc
    11521 agagccacgc cgcgctgctc gagcaactgg cccaactggg tagagagacc cgcgccaaca
    11581 gcgcgctggc cgacaagatc cgccacaagt accggctgaa gaacaccacc ggcttctcgc
    11641 tcaacgcgct ggtcgacttc gacgagccgc tggacatcct caaccacctg atggtcggct
    11701 ccgagggcac gctgggtttc atcagcgcgg tgacctacga caccgtgccc gactacccgc
    11761 acaaggccag cgcgctggtg gtgttccccg acgtggaaaa ctgctgccgc gcggtgccgc
    11821 tgctcaagca gcagccggtg tctgcggtgg agctgctcga ccgccgcagc ctgcgctcgg
    11881 tggaaaacat gcagggcatg ccggtctggg tacgggagct gtccagcggc gcctgcgcgc
    11941 tgctgatcga gtcgcgcgcc gccagccaga gcctgctgca cgagcagatc gcgtggatca
    12001 tggcgtccat cgccgacttc ccggtggaga agcaggtcga cttcagcgag gacccggcgg
    12061 tctacaacca gctgtggaag atccgcaagg acaccttccc ggcggtcggc gcggtgcgcc
    12121 agaccggcac cacggtgatc atcgaggacg tcaccttccc ggtcgagcgc ctggccgagg
    12181 gcgtcaaccg cctgctcgag ctgctcgacc ggcaccacta cgacgaggcg atcctgttcg
    12241 gccatgcgct ggagggcaac ctgcacttcg tgttcaccca gggcttcgac gacccggccc
    12301 aggtggcgcg ctacgaggcg ttcatgggcg aggtggcgca gctggtggcg gtggagttcg
    12361 gcggcgcgct gaaggccgag cacggcaccg ggcgcaacat ggcgccgttc gtcgagctgg
    12421 agtggggcag cgaggcctac gcgctgatgt ggcagatcaa gcgcctgctc gaccccaagg
    12481 gcatcctcaa cccggacgtg gtgctctcgg aggatccgca gatccacctc aagcacctca
    12541 agccgctgcc ggccgccgac gagatcgtcg acaagtgcat cgagtgcggc ttctgcgagc
    12601 cggtgtgccc gtccaacggc ctgaccctga ccccgcgcca gcgcatcgtc atctggcgcg
    12661 acatccaggc gaagaagcgc gccggcatcg acaccacgga gctggagcag gcctaccact
    12721 actacggcct cgatacctgc gccgccaccg gcctgtgcgc ccagcgctgc ccggtgggca
    12781 tcaataccgg cgacctggtg cgcaaactgc gcgccgccga agcaggacac gccggcagcg
    12841 ccacctggct ggcgcgccac ttcgccacgg cgatgcaggg cacccgcctg accctggccg
    12901 ccg
//
LOCUS       DATCUN010000073         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_59220, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000073 DATCUN010000000
VERSION     DATCUN010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59220"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="VIO83_02965"
     CDS             complement(<1..111)
                     /locus_tag="VIO83_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8330275.1"
                     /translation="MIDLRTLRQFVSVAEHESISRAAEALHISASPLSRTI"
     gene            278..1621
                     /locus_tag="VIO83_02970"
     CDS             278..1621
                     /locus_tag="VIO83_02970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8330276.1"
                     /translation="MTSSPLQTAQRKVIRRLIPILMLMYTMAYLDRINIGTAALRMNA
                     ELGITQQVFGLAAGLFFVGYLLMEIPSNIVLHRVGARVWLTRIMVTWGLISACTAFVQ
                     NGTQLILIRMLLGIAEAGLFPGLIYCLALWFPPNLRGRMLVIAVLPWAVIVGTPMSAA
                     IVQYADGLFGFAGWRIMLFVEGLLPVLLGFYSIRYLTSKPEEADWLTPTEQQLIIAAR
                     DADDTAAQAGAEPPLRSTLTQPHLILLSLAYATAQMGFYASLIFTPQIVQEFGRLFGT
                     TLSTIQVGLLSAIPSIFGMVMYWFWVAHSDKTRERVWHAIASFVTAAVGIGILLSVHS
                     LYASIAGLALLTAGLVSGPICIWQLTGIGVRGKTAAVVFAYVNCFGIVGSMFSPYLIG
                     LLKDRTGSYDAGLMFLAGAALCGAVFVFLASRLMQQKQLLRRPDATPGTHDAVAH"
     gene            1675..2376
                     /locus_tag="VIO83_02975"
     CDS             1675..2376
                     /locus_tag="VIO83_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8330277.1"
                     /translation="MIHFDHVCMAGPNVYESTFRLSQETGLGNYDGGFFPHYGLGHRV
                     VPLAPDIYIEVEGLVDQRPVRQGQPLAVFMERQTRDGDRFVGWCLRCDTLEELETFAA
                     HHGTTVDPNTTGRELGRQMMDGSRGFALQAPSATTTWPTGMPNMYFKPGGGVHAASLP
                     VVPGSGSVTPKGLAWIEIGGSRSDLATWLGDVGSPADFPFDVRYNGGSPGLYGMGVDT
                     DRGTVDIRRPPVLID"
BASE COUNT          388 a          923 c          786 g          476 t
ORIGIN      
        1 gatggtgcgg ctcagcggcg aggccgagat gtgcagcgcc tcggcggcgc gggaaatgct
       61 ctcgtgctcg gccacgctga cgaattggcg gagcgtcctc aggtcgatca ggtgcatgca
      121 gcttcctcgt gacgggcgcc gtgagtctac gaattcagac atttcaggtc aaagatcttg
      181 agaaatattg tccttttcag tccatcaagg cgtcacgatc atccgcttca gtcgccgaat
      241 tcttcctcgc cacaaccgcc atccactccg ttccgccatg acttccagcc ccctccagac
      301 cgcgcagcgc aaggtgatcc ggcgcctgat tccgatcctg atgctcatgt acaccatggc
      361 ctatctcgac cgcatcaaca tcggcaccgc ggcgctgcgg atgaacgccg aactcggcat
      421 cacgcagcag gtcttcgggc tggccgcggg gctcttcttc gtgggctatc tgctgatgga
      481 gatcccgagc aacatcgtcc tgcatcgcgt gggcgcgcgt gtctggctca cccgcatcat
      541 ggtcacgtgg ggcctcattt cggcatgcac cgccttcgtc cagaacggca cgcagctcat
      601 cctcatccgc atgctgctgg gcatcgccga ggcgggcctg tttccggggc tcatctactg
      661 tttggcgctg tggttcccgc cgaacctgcg ggggcgcatg ctggtcatcg ccgtgctgcc
      721 ctgggccgtg atcgtcggca cgccgatgtc ggccgccatc gttcagtacg ccgacggcct
      781 ctttggcttc gcgggctggc ggatcatgct gttcgtcgaa ggcctgctgc cggtgctgct
      841 gggcttctat tccatccgtt acctcacctc gaagcccgaa gaggccgact ggctcacgcc
      901 gaccgagcag caactgatca tcgcggcgcg cgatgccgac gacaccgccg cgcaagccgg
      961 tgccgaacca cccctgcgca gcacgctcac gcagccgcac ctgatcctgc tgtcgctcgc
     1021 ctacgccacg gcgcagatgg gcttctacgc cagcctcatc ttcacgccgc agatcgtgca
     1081 ggagttcggc cgccttttcg gcaccacgct ctcgaccatc caggtcggcc tgctgtcggc
     1141 catccccagc atcttcggca tggtgatgta ctggttctgg gtcgcgcatt cggacaagac
     1201 ccgggagcgc gtgtggcacg ccatcgcgtc cttcgtcacc gcggcggtgg gcatcggcat
     1261 cctgctgtcg gtgcacagcc tgtatgcgtc gatcgccgga ctcgcgctgc tgacggccgg
     1321 cctggtgagt ggcccgatct gcatctggca gctcaccggc atcggcgtgc gcggcaagac
     1381 cgccgcggtc gtcttcgcct acgtcaactg cttcggcatc gtgggctcga tgttcagccc
     1441 ctacctcatc ggcctgctga aggaccgcac cggctcctac gacgccggcc tgatgttcct
     1501 cgcgggtgcc gccctgtgcg gcgccgtctt cgtgttcctc gcgagccggc tgatgcagca
     1561 gaagcaactg ctgcggcgcc ccgatgcgac gccggggacg cacgacgcgg tcgcgcactg
     1621 agcgacggcc gccctcctct ccagttcctt cccttcaaca ccaaagcgaa cccgatgatt
     1681 cacttcgatc atgtctgcat ggccggaccc aacgtctacg agtccacctt ccgcctgagc
     1741 caggaaaccg gcctcggcaa ctacgacggc ggcttcttcc cgcactacgg cctcggccac
     1801 cgcgtggtgc cgctcgcgcc cgacatctac atcgaggtcg aaggcctggt cgaccagcgc
     1861 ccggtgcgcc agggccagcc gctcgccgtc ttcatggagc gccagacgcg cgacggcgac
     1921 cgcttcgtcg gctggtgcct gcgctgcgac acgctggaag aactcgagac cttcgccgcg
     1981 caccacggca ccacggtcga cccgaacacc accggccgcg aactgggccg ccagatgatg
     2041 gacggctcgc gcggcttcgc gctgcaggcg cccagcgcca ccacgacctg gccgaccggc
     2101 atgccgaaca tgtacttcaa gccgggcggc ggcgtgcatg cggccagcct gccggtcgtg
     2161 cccggctcgg gcagcgtgac cccaaagggc ttggcatgga tcgagatcgg cggcagccgg
     2221 tcggacctgg ccacgtggct gggcgatgtc ggcagccctg ccgacttccc cttcgacgtt
     2281 cggtacaacg gcggcagccc cggcctctac ggcatgggcg tcgataccga ccgcggcacg
     2341 gtcgacatcc gccggccccc ggttctgatc gactgacgtg ggtcttgttc tggtgggtgc
     2401 cctcctcgat ggcgtcctac acgcccgcgc ccatcacctg ccgtactacc gacaggagtc
     2461 gatcaatgcg cgctctaacg tgcgccagcc gctgcacagc ggtgagcgaa gcctgtgttg
     2521 gcatggcgtg tgaactgctg aatcagttcg gctaccgtcg ccccttctct agc
//
LOCUS       DATCUN010000074        30026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_78164, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000074 DATCUN010000000
VERSION     DATCUN010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 30026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30026
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78164"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /gene="secA"
                     /locus_tag="VIO83_02980"
     CDS             complement(<1..592)
                     /gene="secA"
                     /locus_tag="VIO83_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242999.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY8330278.1"
                     /translation="MFAPLLRKLFGSKNEREVKRMAKAVQAVNVLEEQMVALSDEQLK
                     AKTEEFKARLTQGETLDKLLPEAFAVAREAGKRVMGMRHFDVQLIGGMTLHEGKIAEM
                     RTGEGKTLVGTLAVYLNALEGKGVHVVTVNDYLARRDGNWMRPLYEFLGLTVGIVSPF
                     QDPDEKRAAYAADITYGTNNEFGFDYLRDNMAFSMQE"
     gene            complement(708..1631)
                     /locus_tag="VIO83_02985"
     CDS             complement(708..1631)
                     /locus_tag="VIO83_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8330279.1"
                     /translation="MHIIFMSRRHGAARSLTLAPRQGLTLATMLLGCTLAVGVALGSW
                     VRPQPLPMVPESVGAASGIRPQELVEARAAAQRQLDALGVHLADLQARMTRLDALGER
                     LTELSGLEGGEFDFSQPVGQGGPDELLDAEPFSGNEVLGSLDALAARLDNREQQLEVM
                     QQVLAERRLDDSRLIAGRPVASGYQSSEFGRRRDPFHGGARMHKGVDFAARRGTEIVA
                     VAAGVVTWAGRKSGYGWVVEVSHADGYVTLYGHNQRNLVQVGELVQRGQTLALVGSSG
                     RSTGPHVHFEVQKDGLQIDPATYIARAASDE"
     gene            1706..2161
                     /locus_tag="VIO83_02990"
     CDS             1706..2161
                     /locus_tag="VIO83_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEY8330280.1"
                     /translation="MTFRPLPARAPATLLRGTKTLKHLFGEAQRLGQLQQLLESQLQP
                     AARPHCRVAAFRDGSLLLIVTDGQWATHLRYQQKRLLRQLRNFKEFVNLANILFKVQP
                     TTAERHATGRNPTLSSSAAETLRTAASGIDDPRLRAALERLASHTRPPD"
     gene            complement(2235..3146)
                     /gene="lpxC"
                     /locus_tag="VIO83_02995"
     CDS             complement(2235..3146)
                     /gene="lpxC"
                     /locus_tag="VIO83_02995"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY8330281.1"
                     /translation="MIKQRTLKNIIRATGVGLHSGEKVYLTLKPAPVDTGIVFCRTDL
                     DPVVEIPARAENVGDTTLSTTLVKGDIKVDTVEHLLSAMAGLGIDNAYIELSAPEVPI
                     MDGSAGPFVFLIQSAGLQEQEAPKKFIRIKREVTVHEGEKSATFLPFDGFKVSFEIDF
                     DHPVFRGRTQRACVDFSSTSFVKEVSRARTFGFMRDIEFLRSQNLALGGSVDNAIVVD
                     EFRVLNEDGLRYEDEFVKHKILDAIGDLYLLGTSLIGEFRGHKSGHALNNRLLRTLIA
                     EKDAWEVVTFADASTAPISYMRPAAAV"
     gene            complement(3256..4440)
                     /gene="ftsZ"
                     /locus_tag="VIO83_03000"
     CDS             complement(3256..4440)
                     /gene="ftsZ"
                     /locus_tag="VIO83_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY8330282.1"
                     /translation="MFELADNVQSSAVIKVIGVGGGGGNAVNHMLRCNIEDIDFICAN
                     TDAQALKKVEAKTVLQLGQGITKGLGAGTNPEVGRQAALEDRERIAEILQGANMVFIT
                     TGMGGGTGTGAAPIIAEVAKEMGILTVAVVTRPFPFEGRRRMQIADEGIRALSEHVDS
                     LITIPNEKLLTILGKDASLLSAFAKADDVLAGAVRGISDIMQRPGLMNVDFADVRTVM
                     SEMGMAMMGTGHASGPNRAREATEAAIRNPLLEDINLQGARGILVNITAGLDLSLGEY
                     TEVGSIIEQFTSDHATVKIGAVIDPEMRDELHVTVVATGLGARMEKPAKVVDNGVQAA
                     PSVAPSMAQQGAAVNYRDLDRPTVMRSNPSTGGAAPAVKVNTQEDLDYLDIPAFLRRQ
                     AD"
     gene            complement(4495..5733)
                     /gene="ftsA"
                     /locus_tag="VIO83_03005"
     CDS             complement(4495..5733)
                     /gene="ftsA"
                     /locus_tag="VIO83_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY8330283.1"
                     /translation="MASAHSGKMIVGLDVGTSKVVALVGEVASDGQLEIVGIGTHPSR
                     GLKKGVVVNIESTVQAIQHAIDEAQQMAGCRIHSAFVGVAGSHIRSLNSHGIVAIRDR
                     EVSQADIERVLDAAQAVAIPADQRVLHTLAQDYVIDNQEGVREPLGMSGVRLEAKVHV
                     VTCAVNAAQNIEKCVRRCGLEVDDIILEQLASSSSVLTDDEKELGVCLVDIGGGTTDI
                     AIFTEGAIRHSAVIPIAGDQVTNDIAMALRTPTQYAEEIKIRYACALAKLAGAGQTIK
                     VPSVGDRPPRELSRQALAEVVEPRYEELFCLVQGELRRSGFEDLIPAGIVLTGGTAKM
                     EGAVELAEEIFHMPVRLGLPQGVRGLDDVVRNPAYATGVGLLLYGLRKQGSATFASGV
                     VEESKPPMFERFKRWVQGSF"
     gene            complement(5755..6612)
                     /locus_tag="VIO83_03010"
     CDS             complement(5755..6612)
                     /locus_tag="VIO83_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEY8330284.1"
                     /translation="MIALLRHQRPAPVGPLRKPVPRGASRLVVREPLRKRLPRPSLSG
                     LKRLAWPLLLLGLGFGAYELGQRLLPYADQPIAQVNVQGDLQYVSRAAVAAKVAPFIH
                     SSFFNVDLASMRSELEQMPWIAEAEVRRVWPDQVLIRLEEQLPVARWGDEALLNNQGQ
                     AFAAGEAGGYAHLPQLWGPQRAQQQIMQQYQMLSQLLRPLEFTVARLELRERGSWFLT
                     TGQGLELLLGRDHVVEKLRRFVSVYEKALKEHNTNIARVDLRYPNGLAVAWREQPAAP
                     APGTTGNPQ"
     gene            complement(6617..7558)
                     /locus_tag="VIO83_03015"
     CDS             complement(6617..7558)
                     /locus_tag="VIO83_03015"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020306456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY8330285.1"
                     /translation="MSLQANLDPAIFGRVAVLFGGKSAEREVSLKSGQAVLAALQGAG
                     VDAFGIEVGDDLLQRLAGEKIDRAFIVLHGRGGEDGSMQGLLECLGIPYTGSGVLASA
                     LAMDKLRTKQVWQSLGLPTPRHAVLASAADCERAAAELGFPLIVKPAHEGSSIGMARV
                     ESFEQLLAAWQDARRYDPQVLVEQWISGPEFTVAMLRGEVLPPIRLGTTHSFYDYEAK
                     YLADDTRYQVPCGLDADKENELKALTARACEAVGVRGWARADVMQDAGGQFWLLEINT
                     VPGMTDHSLVPMAARAAGRDFQQLVLEVLADSLEARS"
     gene            complement(7555..9006)
                     /gene="murC"
                     /locus_tag="VIO83_03020"
     CDS             complement(7555..9006)
                     /gene="murC"
                     /locus_tag="VIO83_03020"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY8330286.1"
                     /translation="MADALRGKNSAELRRMRRIRRIHFVGIGGVGMCGIAEVLLNLGY
                     QVSGSDLKASPVTERLESFGAQIFIGHRAENAEQADVLVVSSAINPANPEVANALDRR
                     IPVVPRAEMLAELMRYRHGIAVAGTHGKTTTTSLIASVFAAAGLDPTFVIGGRLNAAG
                     TNAQLGASRYLVAEADESDASFLHLQPMVSVVTNIDADHMSTYGGDFNKLKKTFVEFL
                     HNLPFYGLAVLCVDDPVVREILPQVKRPIVTYGFDEGADLRAIDVRQEGMRTFFTVLR
                     KDREPLEVSVNMPGNHNVLNALATIAIATDEGIDDAAIVAGLSGFQGVGRRFQVYGDL
                     PVDGGSVMLVDDYGHHPREVAAVIKAVRGGWPERRLVMVYQPHRYSRTRDLYDDFVQV
                     LGEANVLLLLEVYPAGEEPIPGADSRQLCHSIRQRGVLDPIYVERGADLAPLLKPLLR
                     AGDILLCQGAGDIGSVAPQLIKHPLFVLQGESK"
     gene            complement(8999..10069)
                     /gene="murG"
                     /locus_tag="VIO83_03025"
     CDS             complement(8999..10069)
                     /gene="murG"
                     /locus_tag="VIO83_03025"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY8330287.1"
                     /translation="MPGNVLIMAGGTGGHVFPALACAREFQARGYSVHWLGTPRGIEN
                     ELVPQAGLPLHRIEISGLRGKGVLSLLKAPLQIAKALWQARKVLRELQPVCVLGMGGF
                     VTGPGGIAARLAGVPLIIHEQNAVAGTANRGLAPFASRVCEAFPETFAASAKRRTTGN
                     PVREELFLATPRQNLARRQARLLVLGGSLGAEPLNKLLPAALAQVSGELRPQVYHQAG
                     KQHAEVTAERYRAAAVDAEVVPFIKDMARAYAWADLVICRAGALTVSELAAAGLPSLL
                     VPLPHAIDDHQTRNAEYLAKAGAAVLLPQHATDAATLAAQLTEVLMHSDKLAAMGATA
                     RRLAKPDATRTVVDICLEVAHG"
     gene            complement(10059..11273)
                     /gene="ftsW"
                     /locus_tag="VIO83_03030"
     CDS             complement(10059..11273)
                     /gene="ftsW"
                     /locus_tag="VIO83_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY8330288.1"
                     /translation="MFSLLRLSPSPLLSRRGVDLDFPLLAGCLALLGLGFVMVSSASS
                     EVAAAQSGNSLYYMIRHLIYLAIGFAAGGLVLLVPVASWQKNGGKLLIAAFILLVLVL
                     VPGIGREVNGARRWIGFGIFNIQPSELAKLFTAIYLGGYLVRRQADVRSSWKGLFMPL
                     IVLGLMAGLLLSEPDFGATVVLVGAGIVMLFLGGVSLWRFLPMVVGVLIIGGVVMTSQ
                     AYRLKRLTNFIDPWADQFGAGYQLSQALIAFGRGEWLGVGLGNSVQKQFYLPEAHTDF
                     VFAVLAEELGMVGALLTVALFVVVTLRALHIGLCAEKEQQYFSAYVAYGVAIQWIGQF
                     LINIGVNVGLLPTKGLTLPFLSYGGSSLVLCCVSLALLLRIDWERRNLLGNAEMEFTE
                     EDFAEEEPRHAR"
     gene            complement(11273..12634)
                     /gene="murD"
                     /locus_tag="VIO83_03035"
     CDS             complement(11273..12634)
                     /gene="murD"
                     /locus_tag="VIO83_03035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY8330289.1"
                     /translation="MTLIASDQFRIVVGLGKSGMSLVRFLARQGVPFAVADTRMSPPE
                     LATLQAEFPQVEVRCGALDVEFLCRASEVYVSPGLPLATAALREAAARGVKLSGDIDL
                     FVRHAKAPIVAITGSNAKSTVTTLVGEMAAAAGKKVAVGGNLGTPALDLLADDIELYV
                     LELSSFQLESTERVNAEVATCLNVSDDHMDRYSGLQTYHLAKHRIFRGARQVVVNRQD
                     PLSRPLIADQVPCWSFGLDKPDFKSFGILEEGGEKFLAFQFSKLMPVRELKIRGAHNQ
                     ANALAALALGHAVGLPLAPMLDTLRRFTGLAHRCQWVRERSGVTFYDDSKATNVGAAL
                     AAIDGLGAEIAGKLLLIAGGDGKGADFTPLRASVAKYCRAVVLLGRDAELIAAALKDA
                     DGRDVVPLLRVATLDEAVQRCAELAAAGDAVLLSPACASLDMFKSFEERGRLFAQAVE
                     ELP"
     gene            complement(12640..13722)
                     /gene="mraY"
                     /locus_tag="VIO83_03040"
     CDS             complement(12640..13722)
                     /gene="mraY"
                     /locus_tag="VIO83_03040"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY8330290.1"
                     /translation="MLLLLAEYLQQFYKGFAVFQYLTLRGILGVLTALTLSLWLGPWM
                     IRTLRSRQIGQSVRDDGPQSHLSKKGTPTMGGALILSAIGISTLLWADLSNRYVWVVL
                     AVTLLFGAVGWVDDYRKVIEKNSRGLPSRWKYFWQSVFGLGAAVFLYVTASTPVETTL
                     ILPLLKTIEIPLGIFFVVLTYFVIVGSSNAVNLTDGLDGLAIMPTVLVGGALGIFCYL
                     SGNAKFAEYLLIPYVPGSGELIVFCGALIGAGLGFLWFNTYPAQVFMGDVGALALGAA
                     LGTIAVIVRQEVVLFIMGGVFVMETLSVVIQVASFKLTGRRVFRMAPIHHHFELKGWP
                     EPRVIVRFWIITVILVLIGLSTLKLR"
     gene            complement(13723..15099)
                     /gene="murF"
                     /locus_tag="VIO83_03045"
     CDS             complement(13723..15099)
                     /gene="murF"
                     /locus_tag="VIO83_03045"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019693561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY8330291.1"
                     /translation="MLEAWMLSSVLGALKGKLVGADAFFNGVSIDSRSIEPGQLFVAL
                     SGERFDGHDYLEQVASKSAAAALVEHEVEEVELPQLVVADTRLALGQLGALNRGAFRG
                     RVAAVTGSSGKTTVKEMLASILRADHGGDSEAVLATRGNLNNDLGAPLTLLELAPQHR
                     SAVIELGASHLGEIAYTVGLARPHVAIITNAGTAHVGEFGGPEKIVEAKGEILEGLAA
                     DGVAVLNRDDPAFATWQQRAGGRRVLSFALHAAADFTARELARDARGCPAFTLASPAG
                     ELRIQLNLLGEHNVSNALAAAAAAHALGVGLEAIRAGLESLQPVKGRAVAQLAPSGMR
                     VIDDSYNANPASIKAAIDILAGFSGRTVLVLGDMGELGAWAEASHREVGAYARDKVSA
                     LYAVGAQMAHAVNAFGAGARHFADQAELIAALTAEQSPETTLLIKGSRSAAMENVVAA
                     FCACAGGH"
     gene            complement(15092..16558)
                     /locus_tag="VIO83_03050"
     CDS             complement(15092..16558)
                     /locus_tag="VIO83_03050"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY8330292.1"
                     /translation="MPMSLLKMFPEARRDALIRELSLDSRTLRPGDLFLAVPGHQSDG
                     RAYIGDAIARGASAIAYEAEGAVDVPQSGAVVMIPVKGLAAQLSAIAGRFYGDPSHNL
                     ELVGVTGTNGKTSVSQLLAQALDLLGQRCGLIGTLGIGFHDNLQCGRHTTPDPLAVQA
                     QLALLTQAGAKAVAMEVSSHGLDQGRVEALDFDVAVFTNLSRDHLDYHGDMQTYGAAK
                     ARLFAWPGLCTRVINLDDLFGRELAAREAPSRLISYSQIDPQATLYCTEAEFSEEGVR
                     ARLITAQGDGLLVSPLLGRFNLSNLLAVVGALMGLNHPLDEILRVLPQLQGPLGRMQR
                     LGGGEQPLVVVDYAHTPDALEKVLEALRPHARGRLLCLFGCGGDRDRGKRPLMAAAVE
                     GLADGVWVTDDNPRSEPAEQIVADIRAGFREPERVTFVAGRGAAIRQLIASASPEDVI
                     LLAGKGHEDYQEIAGVRQPFSDIEQAASALAEREVQDA"
     gene            complement(16558..18294)
                     /locus_tag="VIO83_03055"
     CDS             complement(16558..18294)
                     /locus_tag="VIO83_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011535543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY8330293.1"
                     /translation="MKLDGALYPWRFRLVIVLLLGMVAVICWRIVDLHVFDHDFLKAH
                     GDARSVRHIPIPAHRGLITDRNGEPLAVSTPVTTVWANPRELSAARQHWPALAVALGQ
                     DPQVLSQKLGQNAEREFLYLARGLTPEQGEAVLALKIPGVYSLEEFRRFYPAGEVTAH
                     LIGFTDVDDRGREGIELAFDQWLAGVPGSRQVLKDRRGHLIKDVGVARNAKPGKALAL
                     SIDLRLQYLASRELRNALVENKAKAGSLVILDVQTGEVLAMANQPTYNPNNRRNLQPA
                     AMRNRAMIDVFEPGSTVKPISMLAALESGRWKPGDTVEVYPHSLRIGRFTIRDVSRGG
                     GPVLDLAGILIRSSNVGISKIAFDIGGESIYRVMQQVGLGQDTGLGFPGEQVGNLPNH
                     RKWPQAETATLSYGYGLSVTAIQLAHAYATIANNGRMVPLSLARVDQPANSTQVVPDE
                     VARTVQGMLQQVVEAPGGAHRAQVPGYHVAGKSGTARKTSAGSKGYQQNAYRSLFAGF
                     GPTNNPRLAAVVVIDEPGGEGYYGGLVSAPVFSKVMGGALRLMNIAPDNLPPPLPEAQ
                     QAATVAGNGGRG"
     gene            complement(18294..18587)
                     /gene="ftsL"
                     /locus_tag="VIO83_03060"
     CDS             complement(18294..18587)
                     /gene="ftsL"
                     /locus_tag="VIO83_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEY8330294.1"
                     /translation="MIRRARDSMPSGSLLMLVLFGLVLGSSVAVSWSAHWNRKLLNQQ
                     YAELSVRDKAQAEWGRLILEQSTWTAHSRIEALASEQLQMRIPEPGEVRMVAP"
     gene            complement(18584..19528)
                     /gene="rsmH"
                     /locus_tag="VIO83_03065"
     CDS             complement(18584..19528)
                     /gene="rsmH"
                     /locus_tag="VIO83_03065"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY8330295.1"
                     /translation="MNAATFNHVTVLLREAVEALAVRADGCYLDGTFGRGGHSREILG
                     ELGAEGRLLGFDKDPLAIATGLELAAADSRFQIVQRSFAELGEELAVRGLQGKVDGVL
                     LDLGVSSPQLDDPERGFSFMNDGPLDMRMNPDAGVSAAEWIASAAEEEIARVFYEYGE
                     ERFSRRMARAIVQRRAERPFTRTADLAEVITVANPAWEKGKNPATRAFQGLRIHINNE
                     LGDLERGLEAALEALAVGGRLVVISFHSLEDRIVKQFMRRQVKGEADNLPRGLPVQVA
                     KFDPRLRLLGKPQYASAAELKANPRARSAVMRVAEKLR"
     gene            complement(19530..19985)
                     /gene="mraZ"
                     /locus_tag="VIO83_03070"
     CDS             complement(19530..19985)
                     /gene="mraZ"
                     /locus_tag="VIO83_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003103101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY8330296.1"
                     /translation="MFRGASAITLDAKGRLTMPSRYRDEFMARCAGQLIVTIDAVDPC
                     LSVYPLTEWELIEAKLRELPSLREENRRLQRLLIGNAVDLELDAAGRFLVPPRLRDYA
                     GLDKHAVLVGQLNKFQLWDENGWDARCAEDLKAIKEPGGLPDELRNLTL"
     gene            complement(20293..20640)
                     /gene="rnpB"
                     /locus_tag="VIO83_03075"
     ncRNA           complement(20293..20640)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VIO83_03075"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(20681..21535)
                     /gene="rsmI"
                     /locus_tag="VIO83_03080"
     CDS             complement(20681..21535)
                     /gene="rsmI"
                     /locus_tag="VIO83_03080"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY8330297.1"
                     /translation="MNTPGTLYVVATPIGNLEDISARALRVLREVKLIAAEDTRHSAR
                     LMQHFGIGTPLVACHEHNEREQGSRLVQRLLAGDDLALVSDAGTPLISDPGYHLVRNA
                     RAAGVRVVPVPGACALIAALSASGLPSDRFVFEGFLPARSTARRQRLQAVVEEPRTLI
                     FYEAPHRLLECLADLRELFGAERPAVLGRELTKTFETIKGAPLGELHDWVAADSNQQR
                     GECVLLVEGWQEPVGEEALSAEALRVLDLLLAELPLKRAAAVAAEITGVRKNLLYQQA
                     LLRQNAED"
     gene            21658..23466
                     /locus_tag="VIO83_03085"
     CDS             21658..23466
                     /locus_tag="VIO83_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019372146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEY8330298.1"
                     /translation="MIARLRPLSVLCLAGLLSACAGQQTSGLGELPGKPQASAEQLLL
                     QAQQGKPEQAALLRLSAADQALSQRNLAQAQQILDLVKLDELLPAQQIFASTLTAELA
                     LARNKPKAALQALQHPSFARLGELPVGQQVRSQLARANALQADQQPLAAARERVFIAP
                     LLEGQASANNHEAIWTLVAGLPSSQLSSAGTPGDDLAGWLELSRLARGSATPAQQVAS
                     IAEWQARNPQHPAARQLPQSLVKLQEIATRPLTRIALLLPEQGQLASVGKALRDGFVA
                     AHYQSGDSALNISLHDSSKLTDLDAFYRQAQADGVQLVVGPLEKPLVSQLSQRQQLPI
                     PTLALNYNDGRAEAPAQLFQFGLAAEDEAREVAQRAWEDGMRRAVALVPTGEWGNRVL
                     AAFRKDWEARGGTLIATEHVDQPVRLAQQIADLLKLRDSEARAKRLQSTLGSAVEAQP
                     ARRQDIDFVFLAATPAQARQIKPTLAFQYAGDLPIYATSHLFTGSSDPSQDLDLDGIR
                     FCETPWLLDSSDPLRNQVIGEWPAAAGSLGRLYAMGVDAYRLAPRLSQLQALPSTRLD
                     GLTGSLSLAPGQRIVRRLPWAEYRNGQVQRLESTAQ"
     gene            23463..23837
                     /locus_tag="VIO83_03090"
     CDS             23463..23837
                     /locus_tag="VIO83_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY8330299.1"
                     /translation="MSAPTRQAMGQAAEGWAQTHLEEHGLRLLTRNWRCRLGELDLVM
                     LDGDTVVFVEVRYRRHSAWGGAEGSVDARKRARVSAAAQVFLQQQSRWAHHPCRFDVV
                     AVAPADNSSPPRLNWIRGAFDA"
     gene            23863..24456
                     /locus_tag="VIO83_03095"
     CDS             23863..24456
                     /locus_tag="VIO83_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEY8330300.1"
                     /translation="MDMQSRIRQLFQDSIDTKRQAMEVLIPHIEQASQVMVNALLSEG
                     KILSCGNGGSAGDAQHFSSELLNRFERERPSLPAVALTTDSSTITSIANDYSYNEIFS
                     KQIRALGQPGDVLLAISTSGNSGNVIQAIQAAHDREMTVVALTGRDGGGMASLLLPED
                     VEIRVPARVTARIQEVHLLAIHCLCDLIDRQLFGSEE"
     gene            24453..24986
                     /locus_tag="VIO83_03100"
     CDS             24453..24986
                     /locus_tag="VIO83_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8330301.1"
                     /translation="MKRPTLLLATLGLSLLLAGCGARSIGNKIDDQFIPGRVASEIHS
                     SHADLNDTTSHIVVTSYNGVVLLAGQTPRADLKTLAEQAARRAQGVKLVHNHLQVMQP
                     SSPLARSSDAALTTQIKTAMLADNTVPGSKIKVVTENGMVYLLGVVTRAEANRATAVV
                     QGVGGVQGIVRLFEYVD"
     gene            complement(25059..25457)
                     /locus_tag="VIO83_03105"
     CDS             complement(25059..25457)
                     /locus_tag="VIO83_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEY8330302.1"
                     /translation="MNSSRPYLLRALYEWIVDNNCTPHILVAAEYPGARVPAGYAKDG
                     QVVLNVSPSAVRYLEMGNEVLTFEGRFGGVAQSLYVPVAAVLAIYARENGEGMAFELD
                     EPEEGGPDGGPSGDDEPPRPSGRPSLKVVK"
     gene            complement(25469..26086)
                     /locus_tag="VIO83_03110"
     CDS             complement(25469..26086)
                     /locus_tag="VIO83_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8330303.1"
                     /translation="MAAINRLACYSDPADHYSHRVRIVLAEKAVSAQIIEVASGQCPP
                     QLAEVNPYGSLPTLVDRDLALYEPAVVMEYLDERYPHPPLLPAYPVARANSRLLMHRI
                     QRDWCSLVDRVLDARTPESARVQARKELRESLTGVAALFADKPFFLSEEFSMVDCCLL
                     PILWRLPQLGIELPRQAKPLLDYMERLFARDSFQASLSATERAMR"
     gene            complement(26176..26958)
                     /locus_tag="VIO83_03115"
     CDS             complement(26176..26958)
                     /locus_tag="VIO83_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEY8330304.1"
                     /translation="MKKHFAALILAVLPALSFAAAPLEYPLDKVNVDLTDKAALQDGA
                     RTFANYCMGCHSAQFQRYERVAKDLGIPEKVMLENLVFTGAKIGEHMKTGMQPQDAKV
                     WFGAPPPDLTLVARVRGEDWVYTYLRTFYEDPARPWGVNNKVFPNVGMPNVLASLQGR
                     QVIGCKQVQVVEHGKKVFDPLTGTPVTKEGCDQLTIVPKTGKLSEAEFDEKIKNVVSF
                     LSYSANPVKLESQRIGTYVLLFLAFFFVFAYLLKREYWKDVH"
     gene            complement(26958..28169)
                     /locus_tag="VIO83_03120"
     CDS             complement(26958..28169)
                     /locus_tag="VIO83_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEY8330305.1"
                     /translation="MSKFMEWVDARFPATKMWEDHLSKYYAPKNFNFWYFFGSLALLV
                     LVNQILTGIWLTMSFEPSAEGAFASVEYIMRDVEYGWILRYMHSTGASAFFVVVYLHM
                     FRGLMYGSYQKPRELVWIFGMLIYLALMAEAFMGYLLPWGQMSYWGAQVIISLFGAIP
                     VIGADLTQWIRGDYLISGITLNRFFALHVIALPIVILGLVVLHILALHEVGSNNPDGV
                     DIKKKKDANGIPLDGIPFHPYYTVKDIVGVVVFLFVFCTVIFFFPEMGGYFLEKPNFE
                     AANPFKTPLHIAPVWYFTPFYAILRAVPDKLLGVIAMGGAIAVLFVLPWLDRSPVRSM
                     RYKGWLSKIWLLVFCVAFVILGVLGVLAPTPGRTLLSQVCTVLYFAFFILMPFYTRME
                     KTKPVPERVTG"
     gene            complement(28169..28762)
                     /gene="petA"
                     /locus_tag="VIO83_03125"
     CDS             complement(28169..28762)
                     /gene="petA"
                     /locus_tag="VIO83_03125"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEY8330306.1"
                     /translation="MSNDGVNVGRRRFLVAATSVVGAAGAVGAAVPFVGSWFPSAKAK
                     AAGAPVKVNVGKIEPGQQIVAEWRGKPVFIMNRTDEILANLAKLEGVVADPESKVSDQ
                     PTYVDPKVRSIKPGLLVLEGLCTHLGCSPSFRPEVAPADLGAEWLGGYFCPCHGSRYD
                     LAGRVYKAQPAPLNLPVPPHSYESDDVIVIGVDPEKA"
     gene            complement(29012..29404)
                     /gene="rpsI"
                     /locus_tag="VIO83_03130"
     CDS             complement(29012..29404)
                     /gene="rpsI"
                     /locus_tag="VIO83_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY8330307.1"
                     /translation="MSATQNYGTGRRKTATARVFLRPGTGKISINNRSLDQFFGRETA
                     RMVVRQPLELTANVEKFDIFVTVAGGGVSGQAGAIRHGITRALIEYDETLRSPLRKAG
                     YVTRDAREVERKKIGLRKARKRPQYSKR"
     gene            complement(29420..29848)
                     /gene="rplM"
                     /locus_tag="VIO83_03135"
     CDS             complement(29420..29848)
                     /gene="rplM"
                     /locus_tag="VIO83_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY8330308.1"
                     /translation="MKTFSAKPETVKRDWYVVDAAGKTLGRLATEIASRLRGKHKPEY
                     TPHVDTGDYIVVINAEQVRVTGAKSSDKMYYHHSGFPGGIKSINFEKLIAKAPERVLE
                     TAVKGMLPKNPLGRDMYRKLKVYAGANHPHTAQQPQELKI"
BASE COUNT         5376 a        10196 c         9610 g         4844 t
ORIGIN      
        1 tctcctgcat gctgaacgcc atgttgtcgc gcaggtaatc gaagccgaat tcgttgttgg
       61 tgccgtaggt aatgtcggcg gcgtaggcgg cgcgcttttc gtccgggtcc tggaacgggc
      121 tgacgatgcc caccgtcagg ccgaggaatt cgtacagagg gcgcatccag ttaccgtcgc
      181 ggcgagcgag gtaatcgttg accgtgacca cgtgcacgcc cttgccttcg agggcgttga
      241 gatacaccgc cagggtaccc accagggtct tgccttcgcc ggtacgcatc tcggcgatct
      301 tgccctcgtg cagggtcatg ccaccgatca gctgcacgtc gaagtgacgc atgcccatta
      361 cccgcttgcc ggcctcgcgg gccacggcaa aggcttccgg caacagtttg tcgagggtct
      421 cgccctgggt caggcgcgcc ttgaactctt cagtcttggc tttgagctgc tcgtcggaca
      481 gcgcgaccat ctgctcttcc aggacgttga ccgcctgaac tgccttggcc atgcgtttga
      541 cttcacgctc gttcttgctt ccaaagagtt ttctcaacaa aggcgcaaac atatcgggag
      601 ggtcttccat ctgtggaaat ggagggcact cccggggagt gcccagtgcc gtcactacgg
      661 cagcgaaggg ggcattctac tcggaaacgg cggcgagaaa agacgcctca ctcatcactg
      721 gcagcacgtg cgatataagt ggcgggatcg atctgcaggc cgtctttttg cacctcgaaa
      781 tgcacgtgcg gaccggtgga tcgtccgctg ctgccgacca gggcaagggt ctgaccgcgc
      841 tgcaccagct cgccgacctg caccaggttg cgctggttgt gcccgtacag ggtgacatag
      901 ccatcggcat ggctgacttc gacgacccag ccgtagccgg acttgcgtcc ggcccaggtg
      961 accacgccgg cggccaccgc gacgatctcg gtgccgcgcc gggcggcgaa gtccacgccc
     1021 ttgtgcatgc gcgcaccacc gtggaacgga tcgcggcggc ggccgaactc cgaggactgg
     1081 taaccgctgg ccaccggccg cccggcaatc aggcgcgagt catccagtcg gcgctcggcg
     1141 agcacctgct gcatcacctc cagctgctgt tcgcgattgt cgaggcgcgc cgccagggca
     1201 tcgagactgc cgagcacctc gttgccgctg aagggctcgg catccagcaa ctcgtccgga
     1261 ccgccctgcc cgaccggctg ggagaagtcg aactcgccgc cctccagccc gctcagctcg
     1321 gtcaggcgct cacccagggc gtcgagccgg gtcatgcgcg cctgcaggtc ggccagatgc
     1381 acgccaaggg cgtccagctg ccgctgtgcc gcggcgcggg cctccaccag ctcctgcgga
     1441 cggatcccgc tcgcagcgcc gaccgactcg ggaaccatcg gcaagggctg gggtctcacc
     1501 caagagccca gtgcgacgcc gactgccagc gtgcagccga gcagcatcgt ggccagggtc
     1561 agcccctggc gcggggcgag agtcagcgaa cgtgccgcgc cgtggcggcg actcatgaag
     1621 atgatatgca tggtgcatcc cgctcaagga atcagggcag ccgtttgcta agatggccgc
     1681 tcggtttttc tccaggcgtc ctgccatgac ttttcgtccc ctgccggccc gcgctcccgc
     1741 cacactgctg cgcggaacga aaacgctcaa gcatctgttc ggtgaggcgc agcggctcgg
     1801 ccagctgcag caactgctgg aaagccagct gcaaccggcc gcacgaccgc actgccgggt
     1861 cgccgccttc cgcgacggca gcctgctgct gatcgtcacc gacggccagt gggccaccca
     1921 cctgcgctac cagcagaaac gcctgctgcg ccagctacgg aacttcaagg aatttgtgaa
     1981 cttagcgaac atcctgttca aggttcaacc aacaaccgcc gaaaggcatg ccaccggtcg
     2041 aaatccgacc ctctcttcca gtgccgccga gaccctgcgt accgccgcca gcggtatcga
     2101 cgacccgcgc ctgcgcgccg ccctcgaacg cctggccagc cacacccgac cgcctgactg
     2161 acgcgcccag aaaaaacgcg gacgaaaaaa aggccaccgc aggggtggcc tcaagagaaa
     2221 gggagagtct tgcttcagac agcagctgcc gggcgcatgt aggaaatggg cgcggtgctg
     2281 gcatctgcga aagtcaccac ttcccaggca tccttctcgg cgatcagggt gcgcaggagg
     2341 cggttgttca gggcgtggcc ggacttgtgg ccgcggaact cgccgatcag gctggtgccg
     2401 agcaggtaca ggtcaccgat ggcgtccagg atcttgtgct tgacgaactc gtcctcgtag
     2461 cgcaggccgt cctcgttgag cacgcggaac tcgtcgacca cgatggcgtt gtccacgctg
     2521 ccacccagtg ccagattctg cgagcgcagg aactcgatat cacgcatgaa gccgaaggtc
     2581 cgcgcgcggc tgacctcctt gacgaacgag gtgctggaga aatcgacgca ggcacgctgg
     2641 gtgcgaccgc ggaacaccgg gtgatcgaag tcgatctcga agctcacctt gaagccgtcg
     2701 aacggcagga aggtggcgct cttctcgccc tcatgcacgg tcacttcgcg cttgatgcgg
     2761 atgaacttct tcggcgcttc ctgctcctgc agaccagcgg actggatcag gaacacgaag
     2821 ggacccgcgc tgccatccat gatcggcact tccggtgccg acagctcgat gtaggcgtta
     2881 tcgataccca ggccagccat ggccgacagc aggtgctcaa cggtatccac cttgatatca
     2941 cccttgacca gcgtggtcga cagggtggta tcaccgacgt tctcggcccg ggccggaatt
     3001 tccaccaccg gatcgagatc ggtgcggcag aacacgatcc cggtatccac aggagccggc
     3061 ttcagggtga ggtagacctt ctcccccgaa tgcaaaccga cgccagtggc ccggatgatg
     3121 ttcttcaaag tgcgttgttt gatcatggct ttggccgtat ctgcacaagt tgtgaactgt
     3181 tctcagtagg cggggataat agcagagccc gcctttgctg aaaaccaatc acgtcaatac
     3241 ctctgatata gcgaatcaat cagcctgacg ccgcaggaag gccgggatat ccagatagtc
     3301 cagatcctcc tgggtattga ccttgaccgc cggagcggcg ccaccggtgc tcgggttgct
     3361 gcgcatcacg gtcggacggt cgaggtcccg atagttgacc gctgcgcctt gctgcgccat
     3421 cgacggggcg acggacggag cagcctgcac gccgttgtcg accaccttgg ccggcttctc
     3481 catgcgcgca ccgagaccgg tggcgaccac ggtgacatgc agctcatcgc gcatttccgg
     3541 atcgatgacc gcaccgatct tgacggtggc atggtcggaa gtgaactgct cgatgatgct
     3601 gccgacttcg gtgtactcac ccagcgacag gtcgagaccg gcggtgatgt tgaccaggat
     3661 accgcgggcc ccctgcaggt tgatgtcctc cagcagcggg ttgcggatcg ccgcctcggt
     3721 ggcctcgcgg gcgcggttcg ggccgctggc gtggccggtg cccatcatcg ccatgcccat
     3781 ctcgctcatc acggtacgca cgtcggcaaa gtcgacgttc atcaggccgg gacgctgcat
     3841 gatgtcggag atgccgcgta cggcaccggc cagcacgtcg tcggccttgg cgaaggcgga
     3901 cagcaggctg gcatccttgc cgaggatggt cagcagcttc tcgttgggaa tggtgatcag
     3961 cgagtcgaca tgctcggaca gggcacggat gccctcgtcg gcgatctgca tgcggcgacg
     4021 gccctcgaag gggaacgggc gggtgaccac ggccacggtg aggatgccca tttccttggc
     4081 cacctcggcg atgatcggcg cggcgccggt gccggtgccg ccacccatgc cggtggtgat
     4141 gaacaccatg ttggcgccct ggaggatctc ggcgatgcgc tcgcgatctt ccagcgcagc
     4201 ctgacggccg acttccggat tggtcccggc acccaatccc ttggtgatgc cctggccgag
     4261 ctggaggaca gtcttggcct cgaccttctt caacgcctgg gcgtcggtgt tggcacagat
     4321 gaaatcgatg tcttcgatgt tgcagcgcag catgtggttg accgcgttgc cgccgccacc
     4381 gccgacaccg atgaccttga tgactgcgct cgattggacg ttatccgcta attcgaacat
     4441 attccctctc ctctgccctg ttcttgcttt ttagccgcca gcggcgcttt taactcaaaa
     4501 actgccctgt acccagcgct tgaagcgctc gaacatgggc ggcttgcttt cttcgacaac
     4561 accgctggcg aaggttgccg agccctgctt gcgcaggccg tacagcaaca atccgacccc
     4621 cgtcgcgtag gcggggttgc gcacgacatc gtccaggccc ctgacgccct gcggcaggcc
     4681 cagacgtacc ggcatgtgaa aaatctcctc ggccagctcg accgcacctt ccatcttcgc
     4741 ggtgccaccg gtgagcacga tcccggccgg gatcaggtcc tcgaaaccac tgcggcgcag
     4801 ttcgccctgc accaggcaga acagctcctc gtagcgcggc tcgaccacct ctgccagcgc
     4861 ctggcgcgac agctcgcgcg gcgggcggtc gccgacactc ggcaccttga tggtctgccc
     4921 ggcgccggcc agcttggcca gggcgcaggc atagcgaatc ttgatttcct cagcatactg
     4981 agtcggcgta cgcagggcca tggcgatgtc gttggtgacc tggtcgccgg cgatcggaat
     5041 gaccgcactg tgacgaatcg caccttcggt gaagatggcg atgtcggtgg taccgccgcc
     5101 gatgtccacc aggcacacgc ccagttcctt ctcgtcatcg gtcagcaccg agctggaaga
     5161 cgccagctgc tcgaggatga tgtcatccac ctcgaggccg cagcggcgca cgcacttctc
     5221 gatgttctgc gccgcgttca ccgcgcaggt gaccacgtgc accttggctt ccaggcggac
     5281 gccggacatc cccagcggct cgcgtacgcc ttcctggttg tcgatcacgt aatcctgggc
     5341 cagggtgtgc agcacccgct gatcggccgg aatggccacc gcctgggcgg cgtccagcac
     5401 ccgctcgata tcggcctgac tgacctcgcg atcacgaatg gccacgatgc catgcgagtt
     5461 caggctgcgg atgtggctgc cggccacgcc gacgaacgcc gagtggatcc ggcagccggc
     5521 catctgctgc gcctcgtcga tggcgtgctg gatggcctgc acggtggact cgatattgac
     5581 caccacgccc ttcttcaggc cccgcgaggg atgggtaccg atgccaacga tttccagttg
     5641 gccatcgctc gccacttcgc cgaccagcgc caccaccttg gaggtgccga catccagacc
     5701 gacgatcatc ttgccgctgt gcgcgcttgc catgatccca gttatctcct caagtcactg
     5761 cgggttcccg gtagtaccgg gagccggtgc ggccggttgc tcgcgccacg ccacggccag
     5821 gccgttgggg tagcgcaggt cgacgcgcgc gatgttcgta ttgtgttctt tcagggcctt
     5881 ttcgtagacg ctgacgaaac ggcgcaattt ttccactaca tgatcccgcc cgagcagcag
     5941 ttccagcccc tgcccggtgg tgagaaacca gctgccgcgc tcgcgcagct ccagccgcgc
     6001 cacggtgaac tccagtgggc gcagcagctg gctgagcatc tgatactgct gcatgatctg
     6061 ctgctgtgcg cgctgcggcc cccacaactg cggcagatga gcgtagccgc ccgcctcgcc
     6121 ggccgcgaag gcctggccct gattgttcag cagcgcctcg tcgccccagc gcgccaccgg
     6181 cagctgctcc tccagacgga tcagcacctg gtccggccag acccggcgca cctcggcctc
     6241 ggcgatccac ggcatctgct ccagttcgct gcgcatcgac gccagatcga cattgaagaa
     6301 gctggaatgg atgaacggcg ccaccttggc cgccactgcc gcgcggctga cgtactgtag
     6361 gtcgccctgc acgttgacct gggcgatcgg ctggtcggcg tagggcagca gacgctgccc
     6421 cagctcgtag gcaccgaagc ccaggccgag cagcaacagc ggccaggcga gtcgcttgag
     6481 accgctcagc gacggacgcg gcaggcgctt gcgcagcggt tcgcgcacca ccagtcggct
     6541 ggccccgcgg gggaccggct tgcgcagcgg ccccaccggg gccggacgct ggtgacgcag
     6601 cagcgcgatc atgggatcag ctccttgcct cgagactgtc ggccagcact tccagcacca
     6661 gctgctggaa gtcccggccg gcagcacgcg cggccatggg tacgaggctg tggtcggtca
     6721 tcccggggac ggtgttgatc tccagcagcc agaattgccc accggcatcc tgcatcacgt
     6781 cggcccgggc ccagccacgg accccgaccg cttcgcaggc gcgtgcggtg agcgccttga
     6841 gttcgttttc cttgtccgca tccagaccgc aggggacctg gtagcgggtg tcgtcggcca
     6901 ggtacttggc ctcgtagtcg tagaagctgt gggtggtgcc caggcggatc ggcggcagca
     6961 cctcgccacg cagcatggcg acggtgaact ccgggccgct gatccactgc tccaccagca
     7021 cctgcgggtc atagcgccgc gcgtcctgcc aggcagcgag cagctgctcg aagctctcca
     7081 cccgcgccat gccgatgctc gaaccctcgt gggccggctt gacgatcagc gggaagccca
     7141 gctcggcggc ggcccgctcg cagtcggcgg ccgaagccag caccgcatgg cgcggggtcg
     7201 gcaggccgag gctctgccac acctgcttgg tgcgcagctt gtccatcgcc agagccgagg
     7261 ccagcacgcc gctgccggtg tagggaatgc ccaggcactc gagcaggccc tgcatgctgc
     7321 cgtcttcacc gccgcggcca tgcaggacga tgaaggcacg atcgatcttc tcgccggcca
     7381 gccgttgcag caggtcgtcg ccgacctcga tgccgaaggc atccacacca gcgccctgca
     7441 gcgccgccag cacggcttgg ccggacttca gcgacacctc gcgttcggcg ctcttgccgc
     7501 cgaacagcac ggcgacgcga ccgaagatgg cgggatccag gttggcttgc agactcattt
     7561 cgactcacct tgcagcacga acaacgggtg tttgatcaat tgcggagcaa cgctgccgat
     7621 gtcgccggcc ccctggcaga gcaggatgtc gccggcgcgc agcagcggct tgagcagcgg
     7681 cgccagatcg gcgccacgct cgacatagat gggatcgagc acgccgcgct ggcggatgct
     7741 gtggcacagc tggcggctgt cggcgcccgg aatcggctcc tcgccggccg ggtagacctc
     7801 cagcagcagc agcacgttgg cctcgccgag cacctgcacg aaatcgtcgt agaggtcgcg
     7861 ggtgcggctg tagcggtgcg gctggtagac catcaccagg cgccgctcgg gccagccgcc
     7921 gcgcacggcc ttgatcaccg cggccacttc gcgcggatgg tggccgtagt cgtcgaccag
     7981 catcacgctg ccgccgtcga ccggcaggtc gccgtagacc tggaagcggc ggccgacacc
     8041 ctggaagccg gacagaccgg cgacgatggc cgcatcgtcg atgccctcgt cggtggcgat
     8101 ggcgatggtg gccagggcat tgagcacgtt gtggttgccc ggcatgttca ccgacacctc
     8161 gagcggctca cggtccttgc gcagcacagt gaagaaggtg cgcatgcctt cctggcgcac
     8221 atcgatcgcg cgcaggtcgg caccctcgtc gaagccgtag gtgacgatcg gccgcttcac
     8281 ctgcgggagg atctcgcgca ccaccggatc gtcgacgcag agcactgcca ggccgtagaa
     8341 cggcaggttg tgcaggaact caacgaaggt cttcttcagc ttgttgaagt cgccgccgta
     8401 ggtgctcatg tggtcggcgt cgatgttggt gaccaccgaa accatcggct gcagatgcag
     8461 gaagctggcg tcgctctcgt cggcctcggc caccaggtag cgactggcgc cgagctgggc
     8521 gttggtgccg gcggcattca gccggccgcc gatgacgaag gtcggatcga ggccggcggc
     8581 ggcgaacacc gaggcaatca ggctggtggt ggtggtcttg ccatgggtgc cggccaccgc
     8641 gatgccgtgg cggtagcgca tcagttcggc aagcatctcg gcgcgcggca ccaccgggat
     8701 gcgccgatcg agcgcgttgg cgacctccgg gttggccgga ttgatcgcgc tggacaccac
     8761 cagcacgtcg gcctgctcgg cgttctcggc gcggtggccg atgaagatct gcgcgccgaa
     8821 gctttccagg cgctcggtta ccggcgaggc cttgaggtcg gagccggaca cctggtaacc
     8881 gaggttgagc agcacctcgg cgatgccgca catgcccacc ccgccgatgc cgacgaaatg
     8941 gatgcggcgg atacggcgca tgcgccgcag ttcggcggaa tttttgccgc gcagggcatc
     9001 agccatgggc cacctccagg cagatgtcca ccaccgtgcg ggtcgcatcg ggcttggcca
     9061 ggcggcgcgc agtggcgccc atggccgcga gtttgtcgct gtgcatcaga acctcggtca
     9121 gctgcgcggc cagcgtggcc gcatcagtcg catgttgcgg cagcagcacg gcagcgccgg
     9181 ccttcgccag atattcggca ttacgggtct ggtggtcatc gatcgcatgg ggcagcggca
     9241 ccagcagcga cggcagcccg gcagcggcca gttcgctgac ggtcagcgcg ccggcgcgac
     9301 agatcaccag atcggcccag gcgtaggcgc gggccatgtc cttgatgaac ggcaccacct
     9361 cggcgtcgac cgcggcggcg cgatagcgct cggcggtcac ctcggcgtgc tgcttgccgg
     9421 cctggtggta gacctgcggg cgcagctcgc cggacacctg tgccagcgcc gccggcaaca
     9481 gcttgttcag cggctcggcg cccagactgc cacccagcac cagcagacgg gcctgacggc
     9541 gggccaggtt ttgacgcggt gtggcaagaa acagttcctc acgcaccgga ttgccggtgg
     9601 tgcgccgctt ggcgctggcc gcgaaggtct ccgggaaggc ctcgcagacc cggctggcga
     9661 acggggccag gccacggttg gcggtgccgg ccacggcgtt ctgctcgtgg atgatcagcg
     9721 gcacgccggc cagccgcgcg gcgatgccgc cggggccggt gacgaaacca cccatgccga
     9781 gcacgcatac cggctgcagc tcgcgcagca ccttgcgggc ctgccacagg gccttggcaa
     9841 tctgcagcgg cgccttgagc agcgacagca cacccttgcc gcgcagaccg ctgatctcga
     9901 tacggtgcag cggcagtccg gcctgcggca ccagctcgtt ctcgatgccg cgcggcgtgc
     9961 ccagccagtg cacgctataa ccacgggcct ggaactcgcg ggcacaggcc agcgccggga
    10021 atacgtggcc tccggtgccg ccggccatga tcaacacgtt accgggcatg acgcggctcc
    10081 tcctcggcaa aatcctcttc ggtaaactcc atctcggcat ttcccagcag gttgcgccgc
    10141 tcccagtcga tgcgcagcag cagggccagg ctgacgcagc agagcaccag cgaactgccg
    10201 ccataactga ggaatggcag ggtcaggccc ttggtcggca acaggccgac gttcacgccg
    10261 atgttgatca ggaactggcc gatccactga atcgccacgc cataggccac gtaggcggag
    10321 aaatattgct gctccttctc ggcgcacagg ccgatatgca acgcgcgcag ggtcaccacc
    10381 acgaacagcg ccaccgtcag cagcgcgccg accatgccca gctcctcggc cagcacggcg
    10441 aacacgaagt cggtgtgcgc ctcgggcagg tagaactgct tctgcacact gttgcccagc
    10501 cccaccccca gccattcgcc gcggccgaag gcgatcagcg cctggctcag ttggtagccg
    10561 gcgccgaact ggtcggccca ggggtcgatg aagttggtca ggcgcttgag gcggtaggcc
    10621 tggctggtca tcaccacgcc gccgatgatc agcaccccga ccaccatcgg caggaagcgc
    10681 cacaggctga cgccgccgag gaacagcatg acgatgccag cgccgaccag caccacggtg
    10741 gccccgaagt ccggctcgga gagcagcagg ccggccatca gaccgagcac gatcagcggc
    10801 atgaacaggc ctttccagga cgagcgcaca tccgcctggc gccgcaccag atagcccccc
    10861 agatagatcg cggtgaacag cttggcgagc tcggaaggct ggatgttgaa gatgccgaaa
    10921 ccgatccagc gccgcgcacc gttgacctcg cggccaatgc ccggcaccag caccagcacc
    10981 agcaggatga aggcggcgat cagcagcttg ccgccgttct tctgccagct ggctaccggc
    11041 accaacagga ccaggccgcc ggccgcaaag ccgatggcca gatagatcag gtggcggatc
    11101 atgtagtaca gcgaattgcc cgactgcgct gcggccacct cggacgaggc cgaggacacc
    11161 atcacgaaac cgagaccgag cagcgccaga caacccgcca gcagcgggaa gtccagatcg
    11221 acgccgcggc ggctcagcag cggtgagggc gacaggcgca gcagcgagaa catcagggca
    11281 gctcctctac ggcctgggcg aacaggcggc cgcgctcttc gaagctcttg aacatgtcga
    11341 gactggcgca ggctggcgac agcagcacgg catcgcccgc cgcggccagc tcggcgcagc
    11401 gctgcacagc ctcgtcgagg gtcgccacgc gcagcagcgg caccacgtcg cggccatccg
    11461 cgtccttgag cgcggcagcg atcagctcgg catcgcgccc cagcagcacc acggcgcggc
    11521 agtacttggc tacgctggcg cgcagcggcg tgaagtcggc gcccttgccg tcaccgccgg
    11581 cgatcagcag cagcttgccg gcgatctccg cacccaggcc gtcgatggcc gccagggcgg
    11641 cgccgacgtt ggtcgccttg gagtcgtcgt agaaggtcac gccgctgcgc tcgcgcaccc
    11701 actggcaacg gtgcgccagg ccggtgaagc ggcgcagggt atccagcatc ggcgccagcg
    11761 gcaggccgac ggcatggccc agggccagcg ccgccagggc gttggcctgg ttgtgcgcgc
    11821 cgcggatctt cagctcgcgc accggcatca gcttgctgaa ctggaaggcg aggaacttct
    11881 cgccgccctc ctcaaggatg ccgaacgact tgaagtccgg cttgtccagg ccgaagctcc
    11941 agcacggcac ttggtcggcg atcagcgggc gcgacagcgg atcctggcgg ttcaccacca
    12001 cctggcgggc gccacggaag atgcggtgct tggccaggtg ataggtctgc agaccgctgt
    12061 agcggtccat gtggtcgtcg ctgacgttca ggcaggtggc cacttcggcg ttgacccgct
    12121 cggtgctttc cagctggaag ctggacagct cgagcacgta cagctcgatg tcgtcggcca
    12181 gaagatccag ggccggggta ccgaggttgc cgccgaccgc caccttcttg ccggccgccg
    12241 cggccatctc gccgaccagg gtggtcacgg tgctcttggc gttggagccg gtgatggcaa
    12301 cgatcggcgc cttggcatgg cgcacgaaca ggtcgatgtc gccggacagc ttgaccccgc
    12361 gcgccgccgc ctcgcgcagc gccgcggtgg ccagcggcag gccggggctg acgtacactt
    12421 cgctggcgcg gcagagaaac tccacgtcca gcgcgccgca gcgcacttca acctgaggaa
    12481 actcggcctg cagggtcgcc agctccggcg gactcatgcg cgtgtcggcc acggcaaagg
    12541 gcacgccctg acgggcgagg aagcgcacca gcgacatgcc gctcttgccg aggccgacaa
    12601 cgatgcggaa ctggtcggaa gcgatcagcg tcatccgggt tacctcagct tcaacgtgga
    12661 caggccgatc agcaccagga tcacggtgat gatccagaag cgcacgatca cccgcggctc
    12721 gggccagccc ttgagttcga aatggtggtg gatcggcgcc atgcggaaca cgcggcggcc
    12781 ggtgagcttg aacgaggcga cctggatcac caccgacagg gtttccatga cgaacacccc
    12841 gcccatgatg aacagcacca cttcctggcg cacgatcacc gcgatggtgc ccagcgccgc
    12901 gccgagggcc agcgcaccga cgtcgcccat gaacacctgg gccgggtagg tgttgaacca
    12961 gaggaagccc agcccggcgc cgatcagcgc gccgcagaac acgatcagct cgccggagcc
    13021 cggcacgtag ggaatcagca ggtattcggc gaacttggcg ttgcccgaca ggtagcagaa
    13081 gatgcccagg gcgccgccga ccagtaccgt cggcatgatc gccagaccgt cgaggccgtc
    13141 ggtgagattc accgcgttgc tcgaaccgac gatgacgaag taggtcagca cgacgaagaa
    13201 gatccccagc gggatctcga tggtcttcag caggggcagg atcagggtgg tttccaccgg
    13261 cgtgctggcg gtcacgtaga ggaacaccgc cgcgccgaga ccgaacaccg actgccagaa
    13321 gtatttccag cggctcggca ggccgcgcga gttcttctcg atcaccttgc ggtagtcgtc
    13381 gacccagccg acagcgccga acagcagggt caccgccagc accacccaga cgtagcggtt
    13441 gctcaggtcg gcccacagca gggtactgat gccgatggcg gaaaggatca gcgcgccgcc
    13501 catggtcggc gtgcccttct tcgacaggtg cgattgcggg ccgtcgtcgc gcaccgactg
    13561 gccgatctgc cggctgcgca gggtacggat catccacggc cccagccaca gggacagggt
    13621 cagggcagtg agcacgccga gaattccacg cagggtcaga tactgaaaga ccgcgaagcc
    13681 cttgtagaac tgttgcagat actcggccaa cagcagcagc atttaatgtc ctccagcaca
    13741 ggcacagaac gcagcgacga cgttttccat cgctgcgctg cgcgagcctt tgatcaacag
    13801 ggtggtttcc ggagactgtt cggcagtcag cgcggcaatc agttcagcct gatcggcaaa
    13861 atgccgtgcc ccggcaccga aggcattgac ggcatgggcc atctgcgcgc cgacggcata
    13921 cagggccgag accttgtcac gggcataggc acccacctca cgatggctcg cctcggccca
    13981 ggcacccagc tcgcccatgt cgccgagcac caggacggtg cgtccggaaa agccggccag
    14041 tatatcaatc gcggccttga ttgacgcggg attggcgttg taactgtcgt cgatcacgcg
    14101 catgccgctg ggtgccagct gcgccacggc acggcccttg accggctgca agctttccag
    14161 cccggcacgg atcgcttcca gccccacgcc cagggcatgg gcggcggcgg cggcggccag
    14221 ggcgttgctc acgttgtgct cgccgagcag gttgagctgg atgcgcagct cgccggcggg
    14281 gctggccagg gtgaacgccg ggcagccgcg ggcatcgcgc gccagctcgc gggcggtgaa
    14341 gtcggcagcg gcgtgcagag caaaactcag cacccgccga ccgccggcgc gctgctgcca
    14401 ggtggcaaag gctgggtcgt cgcggttgag caccgccacg ccgtcggccg ccagtccctc
    14461 gagaatctcg cccttggcct cgacgatctt ctccgggccg ccgaactcgc cgacatgggc
    14521 ggtgccggcg ttggtgatga tggcgacgtg cggtcgggcc agaccaacgg tgtaggcgat
    14581 ctcgcccagg tgcgaggcgc ccagctcgat caccgcgctg cggtgctgcg gagccagttc
    14641 gagcagggtc agcggcgcgc ccagatcgtt gttcagattg ccgcgggtgg ccagcaccgc
    14701 ctcgctgtcg ccaccgtggt cggcgcgcag gatgctggcg agcatctcct tgaccgtggt
    14761 cttgccactg gagcctgtga ccgccgcgac ccggccgcgg aaggcgccgc ggttgagcgc
    14821 gcccagctga cccagcgcca ggcgggtatc ggccaccacc agttgcggca gctcgacttc
    14881 ctcgacctca tgctccacca gcgcggcggc ggcactcttg ctggccacct gctcgagata
    14941 gtcgtggccg tcgaagcgct cgccgctgag ggcgacgaac agctgtccgg gctcgatgct
    15001 gcggctgtcg atgctcaccc cgttgaagaa ggcatcggcc ccgaccagct ttcccttcag
    15061 tgctcccagc acactgctca gcatccaggc ttcaagcatc ctgcacctcg cgctcagcca
    15121 gcgcactggc ggcctgttcg atatcggaga agggctgacg caccccggcg atttcctggt
    15181 agtcctcatg gcccttgccg gccagcagga tcacgtcctc cggggaggcg ctggcgatca
    15241 gctggcggat cgccgcgccg cgcccggcga cgaaggtcac ccgctcgggc tcgcggaaac
    15301 cggcacggat gtcggcgacg atctgctcgg ccggctcgct gcgcgggttg tcgtcggtca
    15361 cccacacgcc atcggccagg ccctcgacgg cggccgccat caacggacgc ttgccgcgat
    15421 cgcgatcgcc gccgcagccg aacaggcaca gcaggcggcc acgggcatgc gggcgcaggg
    15481 cctcgagaac cttttccagg gcatcgggcg tgtgggcgta atccaccacc accagcggct
    15541 gttcgccgcc accgaggcgc tgcatgcgcc cgagcggccc ctgcagctgc ggcagcacgc
    15601 ggaggatctc gtccagcgga tggttcaggc ccatcagtgc gccaaccacg gccagcaggt
    15661 tgctcagatt gaagcgaccg agcagcgggc tgactagcag gccgtcgccc tgggcggtga
    15721 tcaggcgggc acgcacgccc tcctcgctga attcggcttc ggtgcagtac agggtcgcct
    15781 gcgggtcgat ctggctgtag ctgatcagcc gcgacggcgc ctcgcgggcg gccagttcgc
    15841 gaccgaacag gtcgtcgagg ttgatcaccc gggtgcacag ccccggccag gcgaacagcc
    15901 gcgccttggc cgcgccatag gtctgcatgt cgccgtggta gtcgaggtgg tcgcgcgaca
    15961 gattggtgaa caccgccacg tcgaagtcca gcgcctcgac ccggccctgg tccagaccgt
    16021 gggaggacac ttccatcgcc accgccttgg cgccggcctg cgtcagcagc gccagctgcg
    16081 cctgcaccgc cagcggatcg ggggtggtgt gacgaccgca ctgcaggttg tcgtggaagc
    16141 cgataccgag ggtgccgatc aagccgcagc gctggccgag cagatcgagc gcctgggcca
    16201 gcagctggct gacgctggtc ttgccgttgg tgccggtcac gccgaccagt tcgaggttgt
    16261 ggctgggatc gccatagaaa cgcccggcga tcgccgacag ctgcgcggcc aggcccttga
    16321 ccggaatcat caccaccgcg cccgactgcg gcacatccac cgcgccttcg gcctcgtagg
    16381 cgatcgccga cgcaccacgg gcaatggcgt cgccgatgta ggcccggcca tcgctttggt
    16441 ggccgggaac agcgagaaac aggtcacccg gacgcaaggt ccggctgtcc aggctcagct
    16501 cgcggatcaa cgcgtcgcga cgggcctcgg gaaacatctt gagcagactc atcggcatca
    16561 gccgcgtcct ccattgccgg ctaccgtggc ggcctgctgc gcttcaggca gcggcggcgg
    16621 caggttgtcc ggagctatgt tcatcaggcg cagggcgccg cccatcacct tgctgaatac
    16681 cggggcggaa accaggccac cgtagtaacc ctcgcccccc ggctcgtcga tcaccacgac
    16741 ggcggcgagg cgcgggttgt tcgtcggccc gaagccggcg aacagcgagc ggtaggcatt
    16801 ctgctggtaa cccttgctgc ccgcgctggt cttgcgcgcg gtaccgctct tgccggcgac
    16861 gtggtagccg ggcacctggg cgcggtgggc gccgccgggg gcctcgacca cctgctgcag
    16921 catgccctgc accgtcctgg ccacctcatc gggaaccacc tgggtggagt tcgccggctg
    16981 atcgacccgc gccaggctca acggcaccat gcggccattg ttggcgatcg tggcgtaggc
    17041 atgcgccagc tggatcgccg tcaccgacag gccgtagccg taagagaggg tcgcggtttc
    17101 cgcctgcggc cacttgcggt ggttgggcag attgccgacc tgctcgccgg ggaagccgag
    17161 gccggtgtcc tgaccgaggc cgacctgctg catgacgcga tagatcgact cgccgccgat
    17221 gtcgaaggcg atcttgctga tgcccacgtt gctggagcgg atcaggatgc cggccaggtc
    17281 gagcaccggg cccccgccgc gggacacgtc gcgaatggtg aagcggccga tgcgcaggct
    17341 gtgcgggtag acctcgacgg tatcgccagg cttccagcgc ccactttcca gcgccgccag
    17401 catcgagatc ggcttcaccg tcgagcccgg ctcgaaaacg tcgatcatgg cgcgattgcg
    17461 catcgcggcc ggctgcaggt tgcggcggtt gttcgggttg taggtgggct gattggccat
    17521 ggccagcact tcgccggtct gcacgtcgag gatcaccaga ctgccggcct tggccttgtt
    17581 ctccaccagg gcgttgcgca gctcgcggct ggccagatat tgcaggcgca ggtcgatgga
    17641 cagagccagc gccttgccag gcttggcatt gcgcgccacg ccgacgtcct tgatcaggtg
    17701 gccacggcgg tccttgagca cctggcgact gccgggcacg ccggccagcc actggtcgaa
    17761 ggccagttcg atgccttcgc ggccgcgatc gtcgacgtcg gtgaaaccga tcaggtgagc
    17821 ggtgacctcg ccggccggat agaagcgccg gaactcctcg aggctgtaga cccccgggat
    17881 cttcagcgcc agcacggcct cgccctgctc gggagtgagc ccacgggcca ggtagaggaa
    17941 ctcgcgctcg gcattctggc cgagcttctg gctcagcacc tgcgggtcct gccccagagc
    18001 gaccgccaga gccggccagt gctggcgcgc cgccgacagc tccctgggat tggcccacac
    18061 ggtagtgacc ggagtactga ccgccagcgg ctcgccatta cggtcggtga tcaggccgcg
    18121 atgcgcggga atcggaatgt gccgcacgct gcgggcatcg ccatgggcct tgaggaagtc
    18181 atgatcgaac acgtgcaggt cgacgatgcg ccagcagatc accgcgacca tgcccagcag
    18241 cagcacgatc accaagcgga agcgccaggg gtagagggca ccgtcgagct tgatcatggc
    18301 gccaccatcc gcacttcacc cggctcgggg atgcgcatct gcagctgctc gctggccagc
    18361 gcctcgatcc ggctgtgcgc ggtccaggtg ctctgctcga ggatcaggcg cccccactcg
    18421 gcctgcgcct tgtcgcgcac gctcagctcg gcgtactgct ggttgagcag cttgcgattc
    18481 cagtgcgcgc tccacgacac ggccacggac gagcccagca ccaagccgaa cagcaccagc
    18541 atcagcagac tgccgctggg catgctgtca cgggcgcgac ggatcatcgc agcttctccg
    18601 ccacgcgcat caccgcgctg cgcgcgcgcg ggttggcctt gagttcagcg gccgaggcgt
    18661 actgcggctt gcccagcagc ctcaggcgag gatcgaactt ggccacctgc accggcaggc
    18721 cgcgcggcag gttgtcggcc tcgcccttga cctgacgacg catgaactgc ttgacgatgc
    18781 ggtcttccag ggaatggaag ctgatcacca ccagtcggcc gccaacggcg agggcttcca
    18841 gcgccgcctc gaggcctcgc tcgaggtcgc ccagttcgtt gttgatatgg atgcgcagcc
    18901 cctgaaaggc acgggtcgcg ggattcttgc ccttctccca ggccgggttg gcgacggtga
    18961 tcacctcggc cagatcggcg gtgcgggtga acggccgctc ggcccggcgt tgcacgatgg
    19021 cccgcgccat gcgccgggag aaacgctctt cgccatattc gtagaatacc cgggcgattt
    19081 cttcctcggc ggcggaggcg atccattcgg ccgcgctgac gccggcgtcg ggattcatgc
    19141 gcatgtccag cgggccgtcg ttcatgaagc tgaagccgcg ctcgggatcg tccagctgcg
    19201 gcgaagacac ccccagatcg aggaggacgc cgtcgacctt gccctgcagg ccacgtacgg
    19261 ccagctcctc gccgagctcg gcgaagctgc gctggacgat ctggaagcga ctgtccgcgg
    19321 cggccagttc gaggccggtg gcgatggcca gcggatcctt gtcgaagccc agcaggcgtc
    19381 cttccgcccc cagctcaccc aggatctcgc ggctatggcc gccacggccg aaggtgccat
    19441 ccagatagca gccgtccgcc cgcacggcga gtgcctcgac ggcttcgcgg agcagaacgg
    19501 tcacgtgatt gaaagtggct gcattcatgt cagagcgtca ggttccgtaa ttcgtccggc
    19561 aggccgccgg gttctttgat ggctttgagg tcttccgcgc agcgcgcatc ccagccgttt
    19621 tcgtcccaca actggaactt gttgagctgg ccgaccagca ccgcatgctt gtccagtccg
    19681 gcgtagtcac gcagccgcgg cggcacgaga aagcgcccgg ccgcatccag ctccagatcc
    19741 accgcattgc cgatcagcag tcgctgcagg cgacggttct cttcgcgcag ggacggcagc
    19801 tcgcgcagct tggcctcgat cagttcccat tcggtcaggg gataaacact cagacaggga
    19861 tcaaccgcat cgatggtcac gatgagctgg ccggcacagc gcgccatgaa ctcgtcgcga
    19921 taccgactcg gcatcgtcag acggcccttg gcgtcaagag ttatggcact ggctccgcga
    19981 aacacggctg cacttccccg tagtggttag tcttctgtga ctggaatatc cacttttacc
    20041 cacttttacc cactacagaa cactatagaa acgccacatc aacccagtca aggcgcgccc
    20101 gacgggaaaa cccttacagg accgcaactt agagaaaaga ccggcagacg ggggacacgc
    20161 agggagcaat gcgaaaaaca aactcgagat acgacaaggc gccacgtatc gaacttaaag
    20221 ctctttatta agagcgatat cttttcgaca ttaaaaccgg agggaactaa ctataaaaag
    20281 cagggagggg gagcggagag ccggcctgta agccgggttt tgtcgaggac agtcattcct
    20341 ctaggaccgc catcactgac ggcctcaagc aacctacccg gatccagcgc gggccacgcc
    20401 aatggatccc tatttggtct tgctccgagt ggggtttacc tagccacgaa ctgttgccag
    20461 acgtgcggtg cgctcttacc gcaccttttc acccttaccg gcgcttgcgc gcttaggcgg
    20521 ttattttctg tggcactttc cgtaggctcg cgcctcccag gcgttacctg gcactctgcc
    20581 ctatggagcc cggactttcc tcccccctgc aagcagggcg gcgactgtcc agccgactct
    20641 ccggcgcgca gggtactcac tgctcgcgcg gataacaagc tcaatcctcg gcgttttgtc
    20701 gcaacagcgc ctgctggtag agcagattct tgcgcacccc ggtgatttcc gccgccacgg
    20761 ccgccgcccg cttgagcggc agctccgcca acagcaggtc gagcacgcgc agcgcttcgg
    20821 cactcagcgc ctcctcaccc accggctcct gccaaccctc gaccagcagt acgcactcgc
    20881 cgcgctgctg gttgctgtcg gccgccaccc agtcgtgcag ctcgccgagc ggcgcaccct
    20941 tgatggtctc gaaggtcttg gtcagctcgc gtcccagcac cgccggacgc tcggcgccga
    21001 acagctcgcg caggtcggcc aagcattcga gcaggcggtg cggcgcctcg tagaagatca
    21061 gcgtgcgcgg ctcctccacc acggcctgca accgctgccg gcgcgcggtg ctgcgcgcgg
    21121 gcaggaagcc ctcgaagacg aagcggtcgg acggcaggcc ggaggccgac agggccgcga
    21181 tcagcgcaca ggctccgggc accggcacca cgcgcacgcc ggcggcgcgc gcgttgcgca
    21241 ccaggtgata gccaggatcg gaaatcaacg gcgtaccggc atccgacacc agcgccagat
    21301 cgtcgcctgc cagcaggcgc tggaccaacc gcgagccctg ctcgcgctcg ttgtgctcgt
    21361 gacaggcgac cagcggcgta ccgatgccga agtgctgcat caggcgagca ctgtgacggg
    21421 tgtcctcggc agcgatcagc ttcacttcgc gcagcacgcg caacgcccga gcgctgatgt
    21481 cttccagatt gccgatcggc gtggccacca cataaagagt acccggagtg ttcacgtcgc
    21541 catcccgtcg tcagtcaaag tgcgtcattg taccgggttt ggccccagca agatcgcacc
    21601 cataccgggg cttgggtaca attacgcctt tcaagcctgc gaagcggaat ccctgacatg
    21661 atcgccagac tgcgccctct ttccgtcctc tgcctggcag gcctgctgag cgcctgtgcc
    21721 ggccagcaga cctccggcct cggcgaactg ccgggcaagc ctcaggccag cgccgagcaa
    21781 ctgctgctgc aggcgcagca gggcaagccg gagcaggccg ccctgctgcg cttgagcgcc
    21841 gccgaccagg cgctgagcca acgcaacctg gcccaggccc agcagatcct cgacctggtc
    21901 aagctggacg agctgctgcc ggcccagcag atcttcgcca gcaccctcac cgcagaactg
    21961 gccctggcac gcaacaagcc caaggccgcc ctgcaggcct tgcagcatcc gagcttcgcg
    22021 cgcctgggcg aactaccggt cggccagcag gtgcgcagcc agctggcccg cgccaacgcc
    22081 ctgcaagccg accagcagcc gctggcggcg gctcgcgaac gcgtgttcat cgccccgctg
    22141 ctggaaggcc aggcatcggc caacaaccat gaagccatct ggaccctggt cgccgggctg
    22201 cccagcagcc agctgagcag cgccggcacc cccggcgacg acctggccgg ctggctggaa
    22261 ctgagccggc tggcgcgcgg cagcgccaca cccgcgcagc aggtcgccag cattgccgag
    22321 tggcaggcac gcaacccgca acatccggcg gcccgccagc tgccgcagtc actggtcaag
    22381 ctgcaggaaa tcgccacccg cccgctgacc cgcatcgccc tgctgctgcc cgagcagggc
    22441 cagctcgcca gcgtcggcaa ggccctgcgc gacggctttg tcgccgccca ttaccagagc
    22501 ggagacagcg ccctgaacat cagcctgcac gacagcagca aactgaccga cctcgatgcc
    22561 ttctaccgcc aggcccaggc cgacggcgtg cagctggtgg tcggcccgct ggagaagccg
    22621 ctggtcagcc aactcagcca acgccagcag ctgccgatcc ccaccctcgc cctcaactac
    22681 aacgacggcc gcgccgaagc accggcccaa ctgttccagt tcggcctggc cgccgaggac
    22741 gaagcgcgcg aagtagccca gcgcgcctgg gaagatggca tgcgccgcgc cgtggccctg
    22801 gtaccgacgg gcgaatgggg caatcgcgtg ctggccgcct tccgcaagga ttgggaagcc
    22861 cgcggcggca ccctgatcgc caccgagcat gtcgaccaac cggtacgcct ggcccagcag
    22921 atcgccgatc tgctcaagct gcgcgacagc gaggcacgcg ccaagcgcct gcagagcaca
    22981 ctgggcagcg cggtcgaggc acagccagcc cgccgccagg acatcgactt cgtgttcctc
    23041 gccgccaccc cggcacaggc ccgccaaatc aagcccaccc tggccttcca gtacgccggc
    23101 gacctgccga tctatgccac ctcgcacctg ttcaccggca gcagcgaccc cagccaggac
    23161 ctcgacctgg acggcatccg cttctgcgaa acgccctggc tgctggacag cagcgacccg
    23221 ctgcgcaacc aggtgatcgg tgaatggccg gccgccgccg gcagcctcgg ccgcctgtat
    23281 gccatgggcg tcgacgccta tcgcctggcg ccgcgcctga gccagctgca ggccctgccg
    23341 agcacccgcc tggatggcct gaccggcagc ctgagcctgg cccccggcca gcgtatcgtg
    23401 cgccgcctgc cgtgggcgga ataccgcaac ggccaggtcc agcgcctgga atcgaccgcg
    23461 cagtgagcgc acccacgcgc caggccatgg gccaggccgc cgaaggctgg gcacaaaccc
    23521 acctcgagga gcacggcctg cgcctgctga cccgcaactg gcgctgccgc ctcggcgagc
    23581 tggatctggt catgctcgac ggcgataccg tagtatttgt cgaagtgcgc taccggcgcc
    23641 atagcgcctg gggcggcgcc gaaggcagtg tcgacgcgcg caagcgtgcc cgcgtcagtg
    23701 ctgccgccca ggttttcctg caacagcaat cgcgctgggc gcaccacccc tgccgcttcg
    23761 acgtggtcgc ggtcgccccc gccgacaaca gctcgcctcc gcggctgaac tggatccgcg
    23821 gcgcgttcga cgcctgaacc gccgcccaac cgaaggtcac ccatggacat gcaatcccgt
    23881 atccgccagc ttttccagga cagcatcgac accaagcgcc aggccatgga agtgctcatc
    23941 ccgcacatcg agcaggccag ccaggtgatg gtcaatgccc tgctcagcga gggcaagatt
    24001 ctctcctgcg gcaacggcgg ctcggccggc gatgcccagc acttttcctc ggagctgctc
    24061 aaccgcttcg agcgcgagcg accgagcctg ccggccgtgg ccctgaccac cgacagctcg
    24121 accatcacct cgatcgccaa cgactacagc tacaacgaga tcttctccaa gcagatccgc
    24181 gccctcggcc agcctggcga cgtgctgctg gcgatctcca ccagcggcaa ttccggcaac
    24241 gtgatccagg ccatccaggc cgcccatgac cgcgagatga ccgtggtcgc cctcaccggt
    24301 cgcgacggcg gcggcatggc ctcgctgctg ctgccggagg atgtcgagat ccgcgtgccg
    24361 gcgcgcgtca ccgcgcgcat ccaggaggtg cacctgctgg ccatccactg cctctgcgac
    24421 ctgatcgacc gccaactgtt cgggagtgaa gaatgaaacg acccaccctg ctccttgcca
    24481 ccctcggcct gagcctgctg ctggccggct gcggcgcccg cagcatcggc aacaagatcg
    24541 acgaccagtt catccctggc cgcgtcgcca gcgagatcca cagcagccat gccgatctca
    24601 acgacaccac ctcgcacatc gtggtgacca gctacaacgg cgtggtcctg ctcgccggcc
    24661 agaccccgcg cgccgacctg aaaaccctcg ccgaacaggc tgcccgccgc gcccaggggg
    24721 tgaagctggt gcacaaccac ctgcaggtca tgcaaccctc ctcgccgctg gcgcgcagca
    24781 gcgacgccgc actgaccacc cagatcaaga ccgccatgct cgccgacaac accgtgcccg
    24841 gctcgaagat caaggtggtc accgagaatg gcatggtcta cctgctgggc gtggtcaccc
    24901 gcgccgaagc caatcgcgcc accgcggtgg tccagggcgt cggcggcgta caaggcatcg
    24961 tgcgcctgtt cgagtacgtc gactgaccct gcgccaggca gcaacaaaaa aggcgatcca
    25021 ggaggatcgc cttttttgtt gcttctgacc gaaccgcttt acttgactac cttcaggctg
    25081 ggccgaccac tggggcgcgg cggctcgtcg tcgcccgacg gacctccatc tggcccgccc
    25141 tcttccggct cgtccagctc gaaggccatg ccctcgccat tctcacgggc atagatggcc
    25201 agcaccgcag cgacaggcac atagaggctc tgcgccacac cgccaaaacg cccctcgaag
    25261 gtcaatacct cgttacccat ctccaggtag cgaaccgcgc tcggcgacac gttgagtacg
    25321 acctgtccat ccttggcata gccggccggc acccgtgcac cgggatactc cgcggctacc
    25381 aggatgtggg gcgtgcagtt gttgtcgacg atccactcgt agagggctcg caacagataa
    25441 gggcgacttg aattcatcat cggattcctc agcgcatcgc gcgctcggta gcgctcagac
    25501 tggcctggaa gctgtcgcga gcgaacagcc gctccatgta gtccagcagc ggtttggcct
    25561 ggcgcggcag ctcgatgccc agctgcggca ggcgccagag aatcggcagc aggcagcagt
    25621 cgaccatgct gaactcctcg ctgaggaaga acggcttgtc ggcgaacagt gccgccaccc
    25681 cggtcaggct ctcgcgcaac tccttgcgtg cctggacccg agcgctctcc ggcgtgcgtg
    25741 catccagcac gcggtcgacc agcgagcacc agtcgcgctg gatgcgatgc atcagcaggc
    25801 ggctgttggc gcgcgccacc gggtaggccg gcagcaaggg cggatgcgga taacgctcgt
    25861 ccagatactc catcaccacg gccggctcgt acagggccag atcgcgatcc accagggtcg
    25921 gcaggctgcc gtaggggttg acctcggcca gttgcggcgg gcactgcccg gacgctacct
    25981 cgatgatttg ggcactcacc gccttctcgg caagcacgat gcgcacccgg tgcgaatagt
    26041 gatcggcggg atcggaataa caggccaggc gattgatcgc agccatgggc tctccctcca
    26101 gtcaggatat tttcggaccg cagaaacaca tgcgcgcccc ggagggcgcg catgtatgag
    26161 gactaggacg acggattagt gaacgtcttt ccagtactca cgcttcagca ggtaggcgaa
    26221 cacgaagaag aacgccagga acagcagcac ataggtaccg atacgctggc tttccagctt
    26281 gaccgggttg gccgagtagc tcaggaagct gacgacgttc ttgatcttct cgtcaaactc
    26341 cgcctcgctc agcttgccgg tcttcggcac gatggtcagc tgatcgcagc cttccttggt
    26401 caccggagtg ccggtcagcg gatcgaacac cttcttgccg tgctcgacca cctggacctg
    26461 cttgcagccg ataacctgac ggccctgcag actggccagc acgttgggca tgccgacgtt
    26521 cgggaacacc ttgttgttca caccccacgg acgcgccgga tcttcataga aggtgcgcag
    26581 gtaggtgtac acccagtcct cgccacgcac gcgggcgacc agggtcagat cgggcggcgg
    26641 agcaccgaac cagaccttgg cgtcctgcgg ctgcatgccg gtcttcatgt gttcgccgat
    26701 cttggcaccg gtgaacacca ggttttccag catcaccttt tccgggatgc cgagatcctt
    26761 ggccacccgc tcgtaacgct ggaactgggc gctatggcaa cccatgcagt agttggcgaa
    26821 ggtacgcgcg ccatcctgca gggcggcctt gtcggtcaga tcgacattga ccttgtccag
    26881 cggatattcg agcggagcgg cagcaaagct aagggccggc agaaccgcaa gaatcaatgc
    26941 agcaaaatgc tttttcatca gccagtcacc ctttccggaa ccggtttggt tttctccatc
    27001 cgggtgtaga acggcatcag gatgaagaag gcgaagtaca ggacagtgca gacttgggac
    27061 agcagggtac ggcccggagt cggcgccagc acgcccagca cgccgaggat cacgaaggct
    27121 acgcagaaca ccagcagcca gatcttgctc agccagccct tgtagcgcat cgagcgcacc
    27181 ggactgcggt ccagccacgg caggacgaac agcacggcaa tcgcaccacc catggcgatg
    27241 acgccgagca gcttgtccgg aacggcgcgc aggatcgcgt agaacggagt gaagtaccac
    27301 accggagcga tgtgcagcgg ggtcttgaac gggttggccg cttcaaagtt cggcttctcg
    27361 aggaagtagc cgcccatttc cgggaagaag aagatcacgg tgcagaacac gaacaggaac
    27421 accaccacgc cgacgatgtc tttcacggtg tagtaggggt ggaacgggat gccatccagc
    27481 gggatgccgt tggcgtcctt cttcttcttg atgtccacgc catccgggtt gttggagccg
    27541 acttcgtgca gcgccaggat gtgcaggacg accaggccca ggatcacgat cggcagcgcg
    27601 atcacgtgca gggcgaagaa gcggttcagg gtgatgccgg agatcaggta gtcaccacgg
    27661 atccactggg tcaggtcggc cccgatgacc gggatcgcac cgaacagcga gatgatcacc
    27721 tgggcgcccc agtaggacat ctggccccac ggcagcagat agcccatgaa ggcttcggcc
    27781 atcagcgcca ggtagatcag catgccgaag atccacacca gctcgcgcgg cttctggtag
    27841 gagccgtaca tcaggccgcg gaacatgtgc aggtacacca ccacgaagaa cgccgaggcg
    27901 ccggtggagt gcatgtagcg caggatccag ccgtactcca catcacgcat gatgtattcg
    27961 accgaggcga aggcgccctc ggcggacggt tcgaagctca tggtcagcca gataccggtg
    28021 aggatctggt tgaccagcac cagcagcgcc agcgagccga agaagtacca gaagttgaag
    28081 ttcttcggag cgtagtactt gctcagatga tcttcccaca tcttggtggc gggaaagcgg
    28141 gcgtcaaccc attccatgaa tttgctcatc aggctttctc cgggtccaca ccgatgacga
    28201 tcacatcatc ggactcatag gagtgcggcg ggaccggcag gttcagcggc gcaggctgcg
    28261 ccttgtagac acggccggcc aggtcgtaac gggagccgtg gcaggggcag aagtagccac
    28321 ccagccactc ggcaccgagg tcggccggag cgacttccgg acggaaggac ggcgagcaac
    28381 ccagatgggt gcacagtccc tcgaggacca gcaggccggg cttgatcgag cggaccttcg
    28441 gatcgacata ggtcggttga tcggaaacct tggactccgg atccgcgacg accccctcga
    28501 gcttggccag attggccagg atttcgtcgg tgcggttcat gatgaacacc ggcttgccgc
    28561 gccattcagc aacgatctgc tgacccggct cgatcttgcc cacgttcacc ttcaccggag
    28621 caccggctgc cttggccttg gcactgggga accatgaccc cacgaacggg accgcagcac
    28681 cgacagctcc agccgccccc accaccgaag tggcggcgac gaggaaccga cgtcggccca
    28741 cattcacgcc gtcattactc attcagtcgt ctcccatcag ctttctgcaa cccgcacagg
    28801 cgggcacatg agttaaaacc agctgcgcaa aaaatccgcc aaatggtaat gaaaagcccc
    28861 ttttctgaca aggcaataac tggaaacaaa ctccgtcacg ccctgcagcg caagcctttc
    28921 acgactgcga cagactgtcg cagacaaacc cggcgcataa aaaaacgccc agatcgcgaa
    28981 gatactgggc gttctgttga agcgtagtcg attaacgctt cgagtactgc ggacgcttac
    29041 gagctttgcg cagaccgatc ttcttacgct cgacctcacg ggcgtcacga gtgacgtaac
    29101 cggctttacg cagcgggctg cgcagggttt cgtcgtactc gatcagagcg cgggtgatac
    29161 cgtgacggat cgcaccagct tgaccgctga caccaccacc agcaacggtg acgaaaatgt
    29221 cgaacttctc gacattcgcg gtcagctcga gcggctgacg taccaccatg cgggcggttt
    29281 cgcggccgaa gaactgatcc aggctgcgat tattgatgga gatcttgcca gtacccggac
    29341 gcaggaagac acgcgcggta gcggtcttgc gacggccggt gccgtagttt tgagtcgccg
    29401 acatattgag cctattccgt taaatcttca gttcttgggg ctgctgagcg gtatgcgggt
    29461 ggttggcacc agcgtacacc ttcagcttgc gatacatgtc gcgacccagc gggttcttcg
    29521 gcagcatgcc tttgaccgcg gtctcgagca cgcgctcggg cgccttggcg atcagcttct
    29581 cgaagttgat cgacttgatg ccacccggga aaccggagtg atggtagtac atcttgtcgg
    29641 agctcttggc accggtcaca cgcacctgct cggcgttgat cacgacgatg tagtcgccgg
    29701 tatcaacgtg cggggtgtac tcgggcttgt gcttaccgcg cagacggctg gcgatttcag
    29761 tggcaagacg acccagggtc ttgccagcag cgtcgacgac gtaccagtcg cgcttaacgg
    29821 tttccggttt cgcagagaaa gttttcattc gttatagcct caggggccac ctgaaatatc
    29881 aagcagcaaa tcatactgga cagtacttgc ctttacaaca aggcaaccgc ggccgtctgc
    29941 agacactaaa tcgggggggg gctcgggtca gtgcgtctgc attcggcaaa atcggcaggt
    30001 taggcattcc caccgagcag gacggg
//
LOCUS       DATCUN010000075         1868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_97127, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000075 DATCUN010000000
VERSION     DATCUN010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97127"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VIO83_03140"
     CDS             <1..494
                     /locus_tag="VIO83_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009047068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY8330309.1"
                     /translation="DLLEAQARLDEARLQLKRTEVRAPVDGYITNLNVFEGDFANRGE
                     PAMALVDRHSFYVYGYFEETRLPLIRVGDKAQMRLMSGEQLSGQVDSFARGIYDRDNP
                     ESRELIADVNPTFNWVRLAQRVPVRIHLDALPEGLELAAGMTCTVIITPPTGEARAVP
                     LRW"
     gene            765..1037
                     /locus_tag="VIO83_03145"
     CDS             765..1037
                     /locus_tag="VIO83_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011169161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2790 domain-containing protein"
                     /protein_id="HEY8330310.1"
                     /translation="MKALTLLALALTGVSCMVAAEQRPANAENQDLQNVEHYSYSMDL
                     DIARVIQPAEIPDICGPAPVQMVYEDHQGQRHTLEYLVMGSGCSNG"
     gene            1208..>1868
                     /locus_tag="VIO83_03150"
     CDS             1208..>1868
                     /locus_tag="VIO83_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HEY8330311.1"
                     /translation="MEFLEHAPRFLFFTGKGGVGKTSIACATAIRLAESGKRVLLVST
                     DPASNVGQVFGIAIGNRITTVPAVANLAALEIDPQAAAQLYRERIVGPARGSLPEDEV
                     RGMEEQLSGACTTEIAAFDEFTALLVDSALTAAYEHIIFDTAPTGHTIRLLQLPGAWS
                     GFLEAGKGDASCLGPLAGLDKQREQYRDAVAALADPERTRLVLVARAQQATLREVART
                     HE"
BASE COUNT          308 a          645 c          625 g          290 t
ORIGIN      
        1 ccgacctgct cgaggcccag gcgcggctgg acgaggcgcg cctgcagctc aagcgcaccg
       61 aggtgcgcgc gccggtggac ggctacatca ccaacctcaa cgtgttcgag ggcgacttcg
      121 ccaaccgcgg cgaaccggcc atggcgctgg tcgaccgcca ctccttctac gtctacggct
      181 acttcgagga aacccgcctg ccgctgatcc gcgtcggcga caaggcacag atgcgcctga
      241 tgagcggcga gcagctcagc ggccaggtcg acagcttcgc ccgcggcatc tacgaccgcg
      301 acaacccgga aagccgcgag ctgatcgccg acgtcaaccc gaccttcaac tgggtgcgcc
      361 tggcccagcg cgtgccggtg cgcatccacc tcgatgcgct gccggagggc ctcgagctgg
      421 cggccgggat gacctgcacg gtgatcatca ccccgccgac cggcgaggcg cgcgcggtgc
      481 cgctgcgctg gtaggcggcc tggggaatcc ggtgccgggc cgggcattgt tgtcgaatca
      541 gacaaaagtc cctgtcggca ttggcggtgt aagcgcgggc tcatctttcc taaaatggcc
      601 gcgccttcta ggcaaaggca gtaacacgga cgggcaagcc agcagtgtgc tgcaagctgc
      661 ttgcccggcc gctgttctcc agtactgcgc aagaaaattt cgccaggtcc cacaagggcc
      721 tgccgaagcc cctcgatgaa gtttcgcccg ataggtaact gtcaatgaaa gcgctgaccc
      781 tgctggctct ggctctgact ggagtctcgt gcatggttgc ggccgaacaa cggccggcaa
      841 atgctgaaaa tcaggatttg cagaacgtcg agcactacag ctattcgatg gatctggata
      901 tcgcccgggt tatccagccg gcggagattc cggatatctg cggccccgcg ccggtgcaga
      961 tggtgtacga ggatcaccag ggccagcggc ataccctgga gtacctggtg atggggagcg
     1021 gctgctccaa cggctgaccg ccccgcgccg cgctcgtatg cagacaaagg ggtgcccgcg
     1081 ggcgcccctt tgtcgttctg gcgattgacg gcgcgcaggg cgcggcggga ggctggcggc
     1141 gtatgctgca ggtctgcatg gacggtgccg tcaccgcctg caccgttgtc cgccccggag
     1201 ccttcgcatg gaatttctcg agcacgcgcc acgcttcctg ttcttcaccg gcaagggcgg
     1261 ggtcggcaag acctcgatcg cctgcgccac cgccattcgt ctcgccgaga gcggcaagcg
     1321 cgtgctgctg gtcagcaccg acccggcgtc caacgtcggc caggtgttcg gcatcgccat
     1381 cggcaaccgc atcaccacgg tgccggcggt ggccaacctc gccgcgctgg agatcgaccc
     1441 gcaggccgcc gcccagctgt atcgcgaacg catcgtcggc ccggcgcgcg gcagcctgcc
     1501 ggaggacgag gtgcgtggca tggaggaaca gctgtccggc gcctgcacca ccgagatcgc
     1561 cgccttcgac gagttcaccg cgctgctggt cgactcggcg ctgaccgccg cctacgaaca
     1621 catcatcttc gacaccgcgc cgaccgggca caccatccgc ctgctgcagc tgcccggcgc
     1681 gtggagcggc ttcctcgagg ccggcaaggg cgatgcctcc tgcctcggcc cgctggccgg
     1741 gctggacaag cagcgcgagc agtaccgcga cgcagtggcg gcgctggccg atcccgagcg
     1801 tacccgtctg gtgctggtgg cgcgcgccca gcaggcgacc ctgcgcgagg tggcgcgcac
     1861 ccatgagg
//
LOCUS       DATCUN010000076         1872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_78242, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000076 DATCUN010000000
VERSION     DATCUN010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78242"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VIO83_03155"
     CDS             complement(<1..207)
                     /locus_tag="VIO83_03155"
                     /inference="COORDINATES: protein motif:HMM:NF023965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-hydroxybutyrate polymerase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8330312.1"
                     /translation="MHALSPLDVQARAALAGVTGSLSPQSALLAWVDWATHLVASPGK
                     QSDLAALGLEQASTLAGYLRECMIA"
     gene            879..1220
                     /locus_tag="VIO83_03160"
     CDS             879..1220
                     /locus_tag="VIO83_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY8330313.1"
                     /translation="MELLTLSIIVLVMLAAALALQRPWQRQLPPWPQRAPFLGGGQPD
                     SHAWSRFHVRYYPMTLLLIAFEMEMMFMYPWAVVYVAEGAKAMMEMGMFLAILSVGIV
                     YAWREGVFRWQ"
     gene            1211..>1872
                     /locus_tag="VIO83_03165"
     CDS             1211..>1872
                     /locus_tag="VIO83_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330314.1"
                     /translation="MAVALLQRLAAGRPAPVFLAVGIGGAWVREALSPDPRIELVDSP
                     RHASVLLVAGSVPTDRQDALRRLHDQLPRPAASLWFRSDPLTELNHARRIDRLDDLAS
                     QAIRLHRTLIDGSTPGDPELLPDQPPHPWQGKGMYGQGGEGMMGGVPYGRPMAMAMMQ
                     DLRDGLQLDSLSFTLGPFYPALPPGLQLKLTLQGDLLQKAMIESSADPVQVSPLFFEA
                     LQR"
BASE COUNT          288 a          637 c          652 g          295 t
ORIGIN      
        1 ggcgatcatg cattcacgca agtagccggc cagggtcgag gcctgttcca gccccagcgc
       61 ggccagatcg gattgcttgc cgggcgaggc gaccagatgg gtcgcccagt ccacccaggc
      121 cagcagggcc gactgcggcg acagcgaccc ggtgacaccg gccagggcgg cccgcgcctg
      181 gacgtccaac ggactgagcg cgtgcatccg gggaaccgga gtgtcggaag gagggtggtt
      241 caccacgggg cccagggaac gaaacgggga aagtgcaacc ttggcccgag ggacgcattg
      301 atttcgatca acgccgctgg cgacgggcca tgactttgcc aggcccctgc atgacgccgg
      361 gcgcagtgtg tatgcctgtg tgtcggaaca ggtgctgccg ggagccgtgc ggcgcggcgc
      421 caggcccgag gccgttctcg gtcctcgccg atgcagcgat ccgggtgcac ggctgcgcgg
      481 ccgcgaacgc tggaaccaag tcggctcacc tgtttgccat ttcgtgcaca aggcatgcgg
      541 gacactccga cccgctgccg gtcgccgcca tgcagcgccc tgccaggcgt caccgcgcgc
      601 caggagggcc cagggcgccg aagctccgcc gaaacggtgt tggctgcggc catcctcgcc
      661 ttcagcggca agcagaccga gcagtcgcgg cgccgccgtc gttcgccaaa ggcgggcatg
      721 ccggccttcc gggcgcgcac cggctcggct tggggcgtgc cgagtgggga ccggcagctg
      781 ccatgtcgcc gctgccttcg cggcacacgg caggcgatcg caggcggcag cgtcgagccg
      841 cccgacagcg catccaccga ccgaggaacg caccgtccat ggaactgctg acactttcga
      901 tcatcgtgct ggtcatgctg gcagccgctc tggccctgca gcgcccctgg cagaggcaac
      961 tgccgccctg gccacaacgc gcaccgttcc ttggcggtgg gcagccggac agccacgcct
     1021 ggagtcgctt ccacgtccgt tattacccga tgacgctgct gctgatcgcc ttcgagatgg
     1081 agatgatgtt catgtacccc tgggcggtgg tctacgtcgc cgagggggcc aaggcgatga
     1141 tggagatggg catgtttctc gccatcctct cggtgggcat cgtctacgcc tggcgtgagg
     1201 gggtgttccg atggcagtag cgctcttgca gcgcctggcg gccggtcgcc cggcaccggt
     1261 cttcctcgcc gtcggaatcg gcggcgcatg ggtgcgcgaa gccctgtcgc cggacccgcg
     1321 catcgagctg gtggactccc ctcggcacgc cagtgtcctg ctggtggccg gcagcgtgcc
     1381 gacggaccga caagacgcgc tgcgccgact gcacgaccag ttgccgcgac cggccgcgag
     1441 cctgtggttt cgcagcgatc cgctgaccga gttgaaccat gcccggcgta tcgaccgact
     1501 cgacgatctt gcaagccaag ccatacgcct gcaccgtacg ttgattgacg gcagcacacc
     1561 gggcgacccc gagctgctgc ccgatcagcc gccgcatccc tggcaaggca agggcatgta
     1621 cggccagggt ggcgagggca tgatgggcgg cgtgccttac gggcggccga tggccatggc
     1681 gatgatgcag gatctgcgcg acgggctgca gctcgactcg ctctcgttca cgctggggcc
     1741 gttctacccc gccctgcccc cgggtttgca gctgaagctg accctgcagg gcgatctgtt
     1801 gcagaaggcg atgatcgaat ccagcgccga cccggtgcag gtctcgccgc ttttcttcga
     1861 ggcgctgcag cg
//
LOCUS       DATCUN010000077         4813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_228457, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000077 DATCUN010000000
VERSION     DATCUN010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228457"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VIO83_03170"
     CDS             complement(<1..724)
                     /locus_tag="VIO83_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY8330315.1"
                     /translation="MSTANLRTAYNYKVVRQFAIMTVIWGIVGMGIGVFIAAQLVWPQ
                     LNFDLPWTSFGRLRPLHTNAVIFAFGGCALFATSFYSVQRTCQTRLFGGPLASFVFWG
                     WQLVILLAAVTLPLGFTSSKEYAELEWPVDILITIVWVAYAVVFFGTIMQRKAKHIYV
                     GNWFFGAFILVTAMLHLVNNMEIPVSLTKSYSLYAGATDAMIQWWYGHNAVGFFLTTG
                     FLGMMYYFVPKQAGRPVYSYRLS"
     gene            776..1468
                     /locus_tag="VIO83_03175"
     CDS             776..1468
                     /locus_tag="VIO83_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEY8330316.1"
                     /translation="MDKLPWTDVDTNQSHKADGGFSVSFAELLWSGPVAGARGRLILA
                     HGAGAPMDSPFMNDIAERLAECGVRVVRFEFPYMTARRRDGGKRPPNPQAQLLDCWRE
                     VWAEVNAREPGKWAIGGKSMGGRMASLLADELQPAALVCLGYPFHAAGKPEKPRVAHL
                     AALRTPTLIVQGERDALGNRERVAGYLLSPAIRVLWLAAADHDLKPLKASGFSHAQHL
                     QEAARAVADFLG"
     gene            complement(1509..4181)
                     /gene="acnA"
                     /locus_tag="VIO83_03180"
     CDS             complement(1509..4181)
                     /gene="acnA"
                     /locus_tag="VIO83_03180"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY8330317.1"
                     /translation="MTHPDSFACRRSLEVDGRTYQYFSLPEAARSLGDIRQLPISLRV
                     LLENLLRCEDGRSVTAADCRAVVQWLHKRSSEQEIQFRPARVLMQDFTGVPGVVDLAA
                     MRDAMARAGGDPQKINPLSPVDLVIDHSVMVDHFADPSAFADNVTIEMQRNHERYAFL
                     RWGQQAFANFRVVPPGTGICHQVNLEYLGQVVWSRADDGATLAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAVMLGQPLSMLIPEVVGVRLAGKLREGVTATDLVLTVTELLR
                     KRGVVGKFVEFFGPGLDHLPLADRATIANMAPEYGATCGFFPVDGITLDYLRLSGREP
                     QRIALVEAYCKAQGLWREAGDEPTFSDTLTLDLASVQPSLAGPKRPQDRVALEDIRAQ
                     FDLLLDLSGRQAERDTDFLVADAHYGLHHGDVVIAAITSCTNTSNPSVLMAAGLLAKK
                     AVERGLSRRPWVKSSLAPGSKVVTDYLAKAGLTPYLDQLGFNLVGYGCTTCIGNSGPL
                     PPPIADCVNANDLIVSSVLSGNRNFEGRVHPQVKANWLASPPLVVAFALAGTTRIDLT
                     REPLGTGSDGQPVYLKDIWPSDAEVAAAVGSVDGAMFRRRYADVFSGDAAWQAIAVGD
                     GATYPWDPTSTYVKNPPFFQHINQPPAALADIAGARILALFGDSITTDHISPAGSIKP
                     SSPAGLYLQSLGVAPEDFNSYGARRGNHEVMMRGTFANIRIHNEMLGGEEGGNTLHQP
                     SGDKLSIYAAAMRYQTEGVPLVVIAGKEYGTGSSRDWAAKGTSLLGVKAVIAESFERI
                     HRSNLVGMGVLPLQFVPGQNRQSLALNGSERLSIRGLGAGLKPRQKLIVEILRGDGTE
                     ERIEVLCRIDTAIELDYFKAGGILHHVLRQLLAG"
     gene            4298..4537
                     /gene="tusA"
                     /locus_tag="VIO83_03185"
     CDS             4298..4537
                     /gene="tusA"
                     /locus_tag="VIO83_03185"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HEY8330318.1"
                     /translation="MSQQPDAILDATGLNCPEPVMMLHNKVRDLPAGGLLKVIATDPS
                     TRRDIPKFCLFLGHELLEQQEEAGTYLYWIRKKAD"
     gene            complement(4534..>4813)
                     /locus_tag="VIO83_03190"
     CDS             complement(4534..>4813)
                     /locus_tag="VIO83_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEY8330319.1"
                     /translation="KHKLTKPYHPWTNGQAERMNRTVKEATIKSFHYPDFDALKAHVR
                     AFVTAYNFAKHLKALRWRTPFKTICDAWTRTPDRFKLDPHHLIPGLHT"
BASE COUNT          800 a         1512 c         1687 g          814 t
ORIGIN      
        1 tggacaggcg gtaggaatag accggacggc cagcctgctt gggcacgaag tagtacatca
       61 tgccgaggaa gccggtggtc aggaagaagc ctacggcgtt gtgaccgtac caccactgga
      121 tcatggcgtc ggtggcaccc gcgtacagcg agtacgactt ggtcaggctg accgggatct
      181 ccatgttgtt gaccaggtgc agcatcgccg tcaccaggat gaaggcaccg aagaaccagt
      241 tgccgacata gatgtgcttg gccttgcgct gcatgatggt gccgaagaac accacggcat
      301 aggcgaccca gacgatggtg atcagaatgt cgaccggcca ctccagctcg gcgtactcct
      361 tggaggaggt gaagccgagc ggcagggtga cggcggcgag caagatcacc agttgccagc
      421 cccagaacac gaagctggcc agcggtccgc cgaacaggcg ggtctggcag gtgcgctgca
      481 ccgagtagaa ggaggtggcg aacagggcgc aaccaccgaa ggcgaagatc accgcgttgg
      541 tgtgcagggg gcgcagacgg ccgaaactgg tccagggcag gtcgaagttc aactgcggcc
      601 agaccagttg ggcggcgatg aatacgccaa tccccatccc gacgattccc cagatcaccg
      661 tcattatggc gaactggcga accaccttgt agttataagc agtcctgagg tttgctgtgc
      721 tcattttcga ggttccacga ctaatgggct tgatcggaaa aagatcagcg tcagcatgga
      781 taaactgcct tggacagatg ttgatacaaa tcaaagccat aaggcggatg ggggctttag
      841 cgtgagtttt gccgaacttt tgtggtcagg gccggtcgcc ggagcgcgcg ggcgactgat
      901 tcttgcccac ggcgccggtg cgccgatgga cagcccgttc atgaacgaca tcgccgagcg
      961 cctggccgag tgcggcgtgc gggtggtgcg tttcgagttt ccctacatga ctgcccggcg
     1021 ccgcgatggc ggcaagcggc cgcccaatcc gcaggcgcag ttgctcgatt gctggcgaga
     1081 ggtctgggct gaggtgaacg cccgcgagcc tggcaagtgg gccattggcg gcaagtccat
     1141 gggcgggcgc atggccagtc tgctggccga cgagttgcag ccggccgcgc tggtctgtct
     1201 cggctacccg ttccatgccg ccggcaagcc ggagaagccg cgggtcgcgc atctggctgc
     1261 cttgcgcacg ccgaccctga tcgtgcaggg cgagcgcgac gcgctgggca accgcgagcg
     1321 ggtggccggc tacctgctgt caccggccat tcgcgtgctc tggctggccg ccgccgatca
     1381 cgacctcaag ccgctcaagg cctccggttt cagccacgcg cagcatctgc aggaggctgc
     1441 acgggcggtg gcggacttcc tcggctagtc gtttctttcg cgcgagggtg gcgggcccac
     1501 gaacaacgct atccggccag cagctggcgc agcacatggt gcaagatgcc acccgccttg
     1561 aagtagtcga gttcgatggc cgtgtcgatg cggcacagca cctcgatacg ctcctcggta
     1621 ccgtcgccgc gcaggatctc cacgatcagc ttctggcgcg gcttgagtcc tgcgcccagg
     1681 ccgcggatgc tcagccgctc gctgccgttc agcgccaggc tctggcgatt ctgtccgggc
     1741 acgaactgca gcggcaacac gcccatgccg accagattgg agcggtgaat gcgctcgaaa
     1801 ctttcggcaa tcaccgcctt gactcccagc aggctggtgc ccttggccgc ccagtcgcgc
     1861 gaggagccgg tgccgtactc cttgccggcg atcaccacca gcggcacgcc ctcggtctgg
     1921 tagcgcatgg cggcggcgta gatcgacagc ttgtcgccgg acggctggtg cagggtgttg
     1981 ccgccttcct cgccgccgag catctcgtta tggatgcgga tgttggcgaa ggtgccgcgc
     2041 atcatcacct cgtggttgcc gcggcgggcg ccgtaggagt tgaagtcctc cggggccacc
     2101 ccgaggctct gcaggtacag gcccgccggc gagctgggct tgatgctgcc ggccggcgag
     2161 atgtggtcgg tggtgatcga gtcgccgaac agggcgagga tgcgtgcccc agcgatatcg
     2221 gccagcgctg ccggcggctg gttgatgtgc tggaagaacg gcgggttctt cacgtaggtg
     2281 gaggtcgggt cccacggata ggtggcaccg tcgcccaccg caatcgcctg ccaggcggcg
     2341 tcgccgctga acacgtcggc gtagcggcgg cggaacatgg cgccgtccac gctaccgact
     2401 gcggcagcca cttcggcatc gctcggccag atgtccttca gatacaccgg ctggccgtcg
     2461 ctgccggtgc ccagcggctc gcgggtgagg tcgatgcgtg tggtgccggc cagggcgaag
     2521 gccaccacca gtggcggcga ggccagccag ttggccttca cctgcggatg cacgcgcccc
     2581 tcgaagttgc ggttgccgga cagcaccgag ctgacgatca ggtcgttggc gttgacgcag
     2641 tccgcgatcg gcggcggcag cggcccggag ttgccgatgc aggtggtgca gccgtagccg
     2701 accaggttga agccgagctg gtcgaggtag ggcgtcaggc cggccttggc caggtagtcg
     2761 gtgaccacct tggagcccgg cgccagcgac gacttaaccc aggggcggcg gctcaggccg
     2821 cgctccaccg ccttcttggc cagcaggccg gcggccatca gcacgctggg gttggaggta
     2881 ttggtgcagg aggtgatggc ggcgatcacc acatcgccat gatgcaggcc gtagtgggca
     2941 tcggcgacca ggaagtcggt atcccgctcg gcctggcgtc cggacaggtc cagcagcagg
     3001 tcgaactggg cgcggatgtc ctccagggcg acgcggtcct gcgggcgctt cggcccggcc
     3061 agcgagggct gcacgctggc caggtccagg gtcagggtgt cgctgaaggt cggctcgtcg
     3121 ccggcctcgc gccacaggcc ctgggccttg caataggcct cgaccagggc gatgcgctgc
     3181 ggctcgcggc cggacaggcg caggtagtcg agggtgatcc cgtcgaccgg aaagaagccg
     3241 caggtggcgc cgtattccgg ggccatattg gcgatggtgg cgcggtcggc caagggcagg
     3301 tgatcgaggc cggggccgaa gaattcgacg aacttgccca ccacgccgcg cttgcgcagc
     3361 agctcggtga cggtcagcac caggtcggtg gcggtcaccc cttcgcgcag cttgccggcc
     3421 aggcgcacgc cgaccacctc gggaatcagc atcgacagcg gctggccgag catcaccgcc
     3481 tccgcctcga tgccgccgac gccccagccg agcacgccca gaccgttgat catggtggtg
     3541 tgcgagtcgg taccgaccag ggtgtccggg taggccaggg tggcgccgtc gtcggcgcgg
     3601 ctccacacca cctggcccag atactccagg ttgacctggt ggcagatgcc ggtgcccggc
     3661 ggcaccacgc ggaagttggc gaaggcctgc tggccccagc gcaggaaggc gtagcgctcg
     3721 tggttgcgct gcatctcgat ggtcacgttg tcggcgaagg cgctgggatc ggcgaagtga
     3781 tcgaccatta ccgagtggtc gatcaccagg tccaccggcg acagcgggtt gatcttctgc
     3841 ggatcgccgc cggcgcgggc catggcatcg cgcatggcgg ccaggtcgac caccccgggc
     3901 acgccggtga agtcctgcat cagcacccgt gccgggcgga actggatttc ctgctccgag
     3961 ctgcgtttgt gcagccattg caccaccgcc cggcagtcgg cggcggtcac gctgcggccg
     4021 tcctcgcagc gcagcaggtt ctccagcagc accctgagcg agatgggcag ctggcggatg
     4081 tcgccgaggc tgcgggcggc ctcgggcagg ctgaagtact ggtaggtgcg accgtcgacc
     4141 tcaaggctgc ggcggcaggc gaaactgtcc gggtgggtca tggtggcgtc ctccagctgg
     4201 cgcgggcttg atccataagt ctagtccgtc cgccgccgct cggttggccg cgccgccact
     4261 ttgcgcgaca atgccggcct gttccgcgga gtccctcatg tcccagcaac ccgacgccat
     4321 tctcgacgcc accggcctca actgccccga gccggtgatg atgctgcaca acaaggtgcg
     4381 cgacctgccg gccggcggcc tgctcaaggt gatcgccacc gatccctcga cccgccgcga
     4441 cattcccaag ttctgcctgt tcctcggcca cgagctgctc gagcagcagg aagaggctgg
     4501 cacctatctg tactggatcc gtaagaaggc ggattaggtg tgtagtcccg ggatgaggtg
     4561 gtgcgggtca agcttgaaac ggtccggcgt tcgagtccag gcatcacaga tcgttttgaa
     4621 cggagtacgc caccgtagag ccttcaggtg cttggcaaag ttgtatgccg taacgaaggc
     4681 ccgaacatgg gctttcagcg catcgaagtc cgggtagtgg aacgacttga tggtggcctc
     4741 cttcacggtg cggttcatgc gctcggcctg tccgttggtc cacgggtgat agggcttggt
     4801 gagcttgtgc ttg
//
LOCUS       DATCUN010000078         8850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_59300, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000078 DATCUN010000000
VERSION     DATCUN010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59300"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VIO83_03195"
     CDS             complement(<1..312)
                     /locus_tag="VIO83_03195"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY8330320.1"
                     /translation="MISEAVVDLSRLQFAMTAMYHFLFVPLTLGLAFLLAIMESVYVM
                     TGKQVYQDMVKFWGKLFGINFALGVTTGLTMEFQFGTNWAYYSHYVGDIFGAPLAIEG
                     LM"
     gene            complement(309..524)
                     /locus_tag="VIO83_03200"
     CDS             complement(309..524)
                     /locus_tag="VIO83_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330321.1"
                     /translation="MPQATPPSPWRIPLVREIAVILLIKLAILLTIKAVWFTEPTVPE
                     NGSQRVSERLLGPAQPQPLPLNEEKPR"
     gene            725..1026
                     /locus_tag="VIO83_03205"
                     /pseudo
     CDS             725..1026
                     /locus_tag="VIO83_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014753630.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            1023..2156
                     /locus_tag="VIO83_03210"
     CDS             1023..2156
                     /locus_tag="VIO83_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY8330322.1"
                     /translation="MRDVRGLQLLLCRFLLLMLLGLAPPFASAQSEPVVVEVFVRDGC
                     PHCSAAKIWLAKLAAERPELRIVLRPVDRDADAREDLLRISRQAGLWPPGVPTFVVDG
                     QVLVGFDDAEHRGAQLLALLADGQPAAASVQSGLFGTLSVERLGLPLFTLALGLLDGV
                     NPCAMWVLLFLLSLLLHLHDRARMALVAGTFVLVSGAVYYAFMAAWLNLFLLVGLSTL
                     LVWALSAVAIAIGLVNLRDGLRPGSDFTLSIPAAAKPGLYARIRYILQADSLLPALAG
                     VAALALVVNFIELLCTAGLPAIYTAVLTQQGLTPLAHYAYLGLYILGYIADDALMVAT
                     AVAALSSHKLSAQAGRGLKILSGLVMLALGAVMLLRPEWLLQG"
     gene            2324..3220
                     /gene="folE2"
                     /locus_tag="VIO83_03215"
     CDS             2324..3220
                     /gene="folE2"
                     /locus_tag="VIO83_03215"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEY8330323.1"
                     /translation="MNSLTLPDIAAQTSSALLPLEHVGMAGIAQPILLHGQRIAARVS
                     TGVSLDDATARGIHMSRLYLALEVLEVEELTPALLARVLDEFLASHAGLSACARLEIR
                     GEALLKRPALVSPLAGWKGYPFSVDARLDDAGLRLALEVEIGYSSTCPCSAALARQLI
                     QRRFAADFAGRTLEPGAVLDWLGSSAGILATPHSQRSTATLRVTLGRQATHLPLVELI
                     ERAEAALGTALQTAVKRADEQAFALANGQNLMFCEDAARRLHTALLGQPGLDAFELRV
                     VHAESLHAHDAVAESRWRREAA"
     gene            complement(3302..4591)
                     /locus_tag="VIO83_03220"
     CDS             complement(3302..4591)
                     /locus_tag="VIO83_03220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8330324.1"
                     /translation="MDFQYGIRPNLEQFLHQLLQVLLVGLTIGMMRTVVPALAESEFG
                     VPKNSFVLLSAFVVAFGLVKGTMNFVAGLLSERLGRKKVLLLGWLVALPIPLLVWYAP
                     DWNWIVLATVLLGINQGLTWSMTQTAKLDITRLDERGLTLGLNEFAGYVGVALAGIVT
                     AYLATLLGARTGLLVFGLAVILLALLLTQLWVKDTLPWARAEAARHLQGARQPMPRYP
                     RDVSAQPGSWEIFALMSWRDRRLAALSQAGLVEKFVDALVWVFYPLFLYRQGLSLPDV
                     GWVIGIYGFVWGGSQLFTGKLSDHVGRYWPNVLGMWVCGTGVAMMLLGQGMSWWSLSA
                     AVSGFGMALLYPNLSAAVADISHPAWRGSAIGIYRFWRDLGYGIGALGLGLAAHLSGE
                     METAFGFVAVSMFVSGALLAWLCEETHPRLNQHATQE"
     gene            complement(4605..5336)
                     /locus_tag="VIO83_03225"
     CDS             complement(4605..5336)
                     /locus_tag="VIO83_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8330325.1"
                     /translation="MFFKQLATREASLSYFFGCAGHGVAVAVDVVAGDEDWFIEEAGK
                     AGVEIRYVIDTHVHADHYSGGRALAQRVGAPYCLHESDQGVVRFPFEPLHDGQRLELG
                     NVKIDVLHTPGHTPDSVCLLVTDARRGEAPWFVITGDTLFVGAVGRPDLAGQEVEMAG
                     VLYDSLHTRLLTLPDELEIFPGHQAGSACGAGLSGKPSSTIGFERRWNEALALGRAAF
                     IAQLTASIPPRPTGMDRIIAANIVA"
     gene            complement(5399..6061)
                     /locus_tag="VIO83_03230"
     CDS             complement(5399..6061)
                     /locus_tag="VIO83_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8330326.1"
                     /translation="MEKRTLKDLLYEQVARIGKAVSSPKRLELLELLAQGEKTVETLA
                     AELSVDIKLTSAHLKALREAHLVASRREGKYVVYRLAAPDVAGLWVTLRQVAEAHLAE
                     LRLTLEQMVAAPDRLAAVGRKELLEQARRGELVVIDVRPVDEYATAHLPHARSLPVAE
                     IEQRLDELPRDKEILAYCRGPFCLFADEAVALLQARGYRARKLSDGVSEWQAAGLPVV
                     AA"
     gene            6643..7101
                     /locus_tag="VIO83_03235"
     CDS             6643..7101
                     /locus_tag="VIO83_03235"
                     /inference="COORDINATES: protein motif:HMM:NF044772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330327.1"
                     /translation="MNPCAIKSVKRPADYQPYQGPQAELRSYGERLYKDTQLSSNGLS
                     CESCHQGGKLFQASFAKAYPHQVAMANNQFGMSQVFLDEMVQICMVAPMASKALGWES
                     QELAALTSYVASLQKSFKPNPGAAKNPCAAKSPCAAKNPCAAKSAPCAAR"
     gene            7162..7938
                     /locus_tag="VIO83_03240"
     CDS             7162..7938
                     /locus_tag="VIO83_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330328.1"
                     /translation="MNAQLPAFDTAWPAPAIELPQPGQLSADERTALLEAALEVGRCQ
                     RALAKAGLNVVGELLKGHDGFYEMNHYPDGDVYDRDSHAQYYYHAHREGAVEHGHFHT
                     FLRGPGIPGHLQPLTALQTSEPWPQGKEAIAHLIAVSMDAWGQPIGLFACNRWVTGET
                     WYPAEAVKQMLPAFSIDHAHPSWPTNLWLTALLKLFRPHIESLIEHRDRVIEAWQTNH
                     PGQDVFEDRALEITGYLPISVEEWSQALAHAQATPPRARS"
     gene            7955..8047
                     /locus_tag="VIO83_03245"
                     /pseudo
     CDS             7955..8047
                     /locus_tag="VIO83_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019752755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
     gene            8121..8726
                     /locus_tag="VIO83_03250"
     CDS             8121..8726
                     /locus_tag="VIO83_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8330329.1"
                     /translation="MEEERELVRRLQQGDPAACDAVIRAHHRFLVAMAVPLVGQGLAD
                     DVAQETWLKAFVAIKGFEGRSRLRTWLARIALNEAHALLRRYKREVSLEGWGADSGSP
                     IASSFSDQGAWSHPPAQWHYDTPEALLTEAELHECIRKHLHRLPSDQQGVVLMRELGG
                     LEFAEIAEATGLSEGNIRVLLHRGRQRMHAMLNHFEEVGTC"
BASE COUNT         1620 a         2948 c         2833 g         1449 t
ORIGIN      
        1 catcaggccc tcgatggcca gcggcgcgcc aaagatgtcg ccgacatagt ggctgtagta
       61 ggcccagttg gtgccgaact ggaactccat ggtcagcccg gtggtgacac cgagggcgaa
      121 gttgatgccg aacagcttgc cccagaactt gaccatgtcc tggtagacct gcttgccggt
      181 catcacgtag accgactcca tgatcgccag caggaaggcc aggccgaggg tcaagggcac
      241 gaacaggaag tggtacatcg cggtcatggc gaattgcagg cgcgacaggt cgacgacggc
      301 ttccgagatc atctcggctt ctcctcgtta aggggcaagg gttgcggttg cgcagggccg
      361 agcaggcgct cgctgacccg ctggctgccg ttttccggca cggtgggctc ggtgaaccag
      421 accgccttga tggtcagcag gatggccagc ttgatcagca ggatgacggc gatctcgcgg
      481 accagcggga tgcgccaggg ggatggcgga gtggcttgcg gcatagcgaa agctcctgac
      541 ttgctctcta ccgttgggta cagcggaccc ggactgatca ggtttcagca gcaagggtcc
      601 cggtctcaag tattagcaca tatgcaataa agacatatat ttaggcgaaa acattccatc
      661 aagtttgcac caaaaatacc ccacggagta tgtatggaga ggggtaagag acacttggtc
      721 gcagctgtac gaggccttgc tcgaatgcca ccgggactca tcggccaatc gtattcgtga
      781 ggttgacgta ctgcgcgacg tgcgcgaagc cgacggattc gacgccgggc acatccccgg
      841 cactgccggc tcgcaatctg aagattgtgc tgtactgcaa gacgagcggc cgagccgccc
      901 tcgccgcttg ccccctgcac gacatgggct acctgcaggt gcagatcgtg gggcggcttc
      961 gatgcctgga gcgcagccgg caagatggtg gtcgtcccta gcctgaaagg gagtgagctc
     1021 gcatgaggga tgtgcgcggt ctgcagctgc tgctgtgtcg atttctattg ctgatgttgc
     1081 tgggcttggc gccgccgttc gcctcggcgc aatccgagcc agtggtcgtc gaggtgttcg
     1141 tccgcgatgg ctgcccgcac tgcagcgcgg ccaagatctg gctcgccaag ttggctgcgg
     1201 agcgaccgga actgcgcatc gtgctgcgcc cagtggatcg tgatgctgat gcgcgcgagg
     1261 atctgctgcg tatctcccgt caggctgggc tctggccgcc aggggtgccg accttcgtcg
     1321 tggacgggca agtcctggtg ggcttcgacg acgccgagca ccgcggcgca caactgctcg
     1381 ccttgctcgc tgacggtcag cctgcagccg ccagtgtcca gagcgggctg ttcggcaccc
     1441 tcagcgtcga gcgcctgggc ctgccgctgt tcaccctcgc cctcggcctg ctcgacggcg
     1501 tcaacccctg cgccatgtgg gtgctgcttt tcctgctctc gttgctgctg cacctgcacg
     1561 accgcgcgcg catggcgctg gtcgccggca ccttcgtgct ggtcagtggc gcggtctact
     1621 acgccttcat ggccgcctgg ctcaacctgt tcctgctggt cggcctgtcg acgctgctgg
     1681 tctgggcgct gagcgccgta gccatcgcca tcggcctggt caacctgcgc gacggcctgc
     1741 gtccgggcag cgacttcacc ctgtcgattc cggctgcggc caagcccggg ctgtacgcgc
     1801 gcatacgcta catcctgcag gccgacagcc tgctgcccgc cctcgccggg gtggcagctc
     1861 tggccttggt ggtcaacttc atcgaactgc tgtgcaccgc cggcctgcca gcgatctata
     1921 ccgcggtact gacccagcag ggcctgacgc cgctggccca ctacgcctac ctaggcttgt
     1981 acatcctcgg ctacatcgcc gacgacgcgc tgatggtcgc cacggcggtg gccgccctga
     2041 gcagtcacaa gctgtcggca caggccgggc gcgggctgaa gatcctcagc ggcctggtca
     2101 tgctggcgct cggggccgtc atgctgctgc gccccgaatg gctgctgcag ggctgaacct
     2161 ggccaggcgt ttcctgggtg cggcaagagc acccaggaaa ccggacagga cgtgacgggg
     2221 agacgggaat ccatcgggga cctcggattg gcatactgct gcgcttggcc aatcaagggt
     2281 tataacataa cattatctcc catctgccca ccaaggattg ccgatgaaca gcctgaccct
     2341 gccggatatc gccgcgcaaa cctcctctgc cctgctgccg ctggagcacg tgggcatggc
     2401 tggcatcgcc cagccaatcc tcctccacgg ccagcgcatc gccgcgcgag tcagcaccgg
     2461 ggtcagcctc gacgacgcca ccgcgcgcgg catccacatg tcgcggctgt acctggcgct
     2521 ggaagtcctg gaggtggagg aactgacccc ggcgctgctg gccagggtgc tcgacgagtt
     2581 ccttgccagc catgccgggc tgtcggcgtg cgcccggctg gaaatccgcg gcgaggcgct
     2641 gctcaagcgc ccggcgctgg tcagcccgct ggccggctgg aagggctatc cgttcagcgt
     2701 cgacgcgcgc ctggatgacg cgggcctgcg cctggcgctg gaggtggaga tcggctactc
     2761 gtcgacctgc ccgtgctcgg cggcgctggc ccgccaactg atccagcggc gcttcgccgc
     2821 cgacttcgcc ggccgcacgc tggagccggg cgcggtactg gactggctgg gcagcagcgc
     2881 cggcatcctc gccaccccgc acagccagcg cagcacggcg accctacggg tgaccctggg
     2941 ccggcaggcg actcacctgc cactggtcga gctgatcgag cgcgccgagg ctgcgctggg
     3001 caccgccctg cagaccgcgg tcaagcgcgc cgacgagcag gccttcgccc tggccaacgg
     3061 acagaacctg atgttctgcg aggacgccgc acggcgcctg cataccgcgc tgctcggcca
     3121 gccggggctg gacgccttcg aactgcgcgt ggtgcatgcc gagagtctgc acgcccacga
     3181 cgcggtggcc gagagtcgct ggcgccggga ggcggcatga tccgtgccag tacgctgcag
     3241 tggaggcctg ctgatccgcg ccgacggctg ccgctgcggg ctgaatcagg gaaatggctc
     3301 atcactcctg agtcgcatgc tgattgagcc ttggatgggt ctcttcacac agccaggcaa
     3361 ggagcgcacc ggaaacgaac atcgacacgg cgacgaaccc gaacgccgtc tccatctcgc
     3421 cgctgaggtg agccgccagc cccaggccga gggctccgat gccgtagccc aggtcgcgcc
     3481 agaagcggta gatgccgatg gccgagccac gccaagccgg atgggagatg tcagccacgg
     3541 cagcagacaa gttcgggtag agcagcgcca tgccgaagcc cgagacagcc gccgacagac
     3601 tccaccagga cataccttgg cccagcagca tcatcgccac gcccgttccg cagacccaca
     3661 tgcccagcac gttgggccaa taacggccga cgtggtcgga aagcttcccg gtgaacagct
     3721 gcgagccacc ccagacgaag ccatagatgc caatgaccca gccgacatcc ggcaggctga
     3781 gtccctgacg gtaaaggaac agcggataga agacccagac cagcgcatcg acgaacttct
     3841 cgaccaaccc cgcctggctg agcgcagcca gccggcggtc gcgccaggac atgagggcga
     3901 agatttccca gctgcccggc tgcgccgata catcgcgcgg ataacgcggc atgggctgcc
     3961 ttgctccctg tagatgtcgt gccgcttcgg cccgtgccca aggcagggtg tccttgaccc
     4021 agagctgagt cagcagcagg gccagcagga tcaccgccaa gccgaacacc agcagcccgg
     4081 tccgcgcgcc cagcagggtc gccaggtagg ctgtcacgat gcccgccagg gccacaccta
     4141 catagccggc gaactcgttg aggcccaggg tcaggccgcg ctcgtcgaga cgggtgatgt
     4201 ccagcttggc ggtctgggtc atcgaccagg tcaacccttg gttgatgccg agcagtacgg
     4261 tggccagcac gatccagttc cagtcggggg cgtaccagac cagcagaggg atcggcagcg
     4321 cgaccagcca acccagcagc agcactttct tgcgccccag gcgctcggaa agcaggccag
     4381 cgacgaagtt catcgtacct ttgaccagcc caaacgcgac cacgaaggcg ctgagcagga
     4441 cgaaggagtt cttcggcaca ccgaactcgg actcggccag cgccggcacc accgtgcgca
     4501 tcatgccgat ggtcagaccg accagcagga cctgtagcaa ttggtggagg aactgctcca
     4561 ggttggggcg gatgccgtac tgaaaatcca tgctgcctcc ctgctcacgc cacgatgttg
     4621 gccgcgatga tgcgatccat gcctgtcggg cgcggcggaa tcgacgcggt cagctgggcg
     4681 atgaaagcgg cacgacccaa ggccagggcc tcgttccagc gcctctcgaa gccgatggtc
     4741 gaggatggct tgccggacag gcctgcgccg caggcgctgc ccgcctggtg gcccgggaaa
     4801 atctccagct cgtcgggcag ggtcagcaac cgggtatgca ggctgtcgta cagcacgcca
     4861 gccatttcca cctcctgtcc ggccagatcc ggacgcccaa cagcgccgac gaacagggtg
     4921 tctccggtga tcacgaacca cggggcttca cctcgtcgcg catcggtgac cagcagacag
     4981 acactgtctg gcgtgtgccc cggagtatgc aggacatcga tcttgacgtt gcccagctcc
     5041 aagcgttggc catcgtgcaa tggctcgaaa gggaaacgca ctacgccctg gtcagactcg
     5101 tgcaggcagt agggcgcccc aacccgctga gccagcgccc ggccccccga gtagtgatcg
     5161 gcatgcacat gggtgtcgat cacatagcgg atctccactc cagccttacc ggcctcctcg
     5221 atgaaccagt cctcatcgcc ggccaccaca tcgacggcaa ccgctacgcc atgccccgca
     5281 cagccgaaga aataggacaa ggacgcctcg cgggtcgcca gttgcttgaa aaacattggc
     5341 aatactcctt cagaacacca gaaccttgtc cgcctcgacg gtccagtcgg ccagtcactc
     5401 aggcggcaac caccggcagg ccggccgcct gccactcgct cacgccatcg gacagcttgc
     5461 gtgcgcgata gcctctggcc tgcagcagcg caaccgcctc gtcggcgaac aggcagaacg
     5521 gcccacggca gtaggcgagg atctccttgt cgcggggcag ttcgtcgaga cgttgctcga
     5581 tctcggccac cggcagcgag cgcgcatggg gcagatgggc cgtggcgtac tcgtccacag
     5641 gccgtacatc gatgaccacc agctcgccgc ggcgagcctg ttcgagcagt tctttcctgc
     5701 ccactgcggc aagtctgtcc ggggcggcga ccatctgttc cagggtcagg cgcagttccg
     5761 ccagatgcgc ctccgccacc tggcgcagcg tgacccacag tcccgccaca tcgggcgcgg
     5821 ccagccggta gaccacgtac ttgccctccc gccgggaggc gaccagatgg gcctcgcgca
     5881 gcgccttgag gtgggcactg gtgagcttga tgtcgaccga cagctcggca gccagcgtct
     5941 cgacggtttt ttcgccctgg gccagcagtt cgaggagctc caggcgcttg gggctggaga
     6001 cggccttgcc gatgcgggcc acctgttcgt agagcagatc tttgagtgtt cgcttttcca
     6061 ttcgctcaat ctaatgatca ttggaatgta tggcaagacc taatcgccga ggggctgcca
     6121 tcagcactgc tcgcgtagac gagaggcttg ccagcgcccg gccacagcag cagcgacgtg
     6181 acgatggcag ttgaggccgg aaaacggccg ggcattttcg ccaggctggg tgtaacaagc
     6241 gacgagcatc tgcatccgag gggtgacggt tcagtggagc gctaccgaag gccacgcgca
     6301 ggctgaccgt gacgagctgg ttgctccttc cgacaacgaa accagcgatg gcgttggaac
     6361 cttggtgccc gatgagaatg ctcacaagag gaacacccca tgcgctactc ctgtcccgac
     6421 cagccgcaac gcggctgtca tcgccccatc accgccatgg tgtcggccag cgtactggca
     6481 atggccctgg cggggccggc gctcgccgcc ccctgcgccc cgtgtgccgc caaatgcgcg
     6541 ccggcgagcc tgaatccctg tgctgccaag agccccggcg cggcgaaaaa cccttgtgcc
     6601 gccaaaggcg ctccctgcgc cgccaaccct tgcgcggcga agatgaatcc ctgcgccatc
     6661 aagtcggtca agcgaccggc cgactaccag ccctaccagg gcccgcaggc cgaactcagg
     6721 agctacggcg agcggctcta caaggacacc cagctgagca gcaacggcct ttcctgcgag
     6781 agctgccacc aagggggcaa gctattccag gccagcttcg ccaaggccta cccccatcaa
     6841 gtcgccatgg ccaacaacca gttcggcatg agccaggtat tcctcgacga gatggtgcag
     6901 atctgcatgg ttgcaccgat ggcgagcaag gcgcttggct gggagtcgca ggagctggcg
     6961 gcgctgacga gctatgtggc cagcctgcaa aagagcttca agcccaaccc cggcgcggcc
     7021 aaaaatcctt gtgcggcgaa aagcccctgc gctgccaaga acccctgcgc tgcgaagtcg
     7081 gcgccctgcg cagccaggta actgacgcgc cttgagcgga gcggccgctc ccccgctccg
     7141 gtcctttttc gaggttccga catgaatgcc cagctacctg ccttcgatac cgcctggccc
     7201 gcgcctgcga ttgaactgcc ccagcccggg cagctgagtg ccgacgagcg taccgccctg
     7261 ctggaagctg ccctggaagt cggccgctgc cagcgggcgc tggccaaggc gggattgaac
     7321 gtagtcggcg agttgctcaa ggggcacgac ggtttctatg agatgaacca ctacccggat
     7381 ggcgacgtat acgaccggga cagccatgcc cagtactact accacgccca ccgcgagggg
     7441 gcggtcgagc atggccactt ccataccttc ctgcgtgggc ctggcatccc tgggcatctg
     7501 cagccgttga cggcactgca gaccagcgag ccctggcctc agggcaaaga agccatcgcg
     7561 catctgatcg ccgtatcgat ggatgcctgg ggccagccga tcgggctgtt cgcctgcaac
     7621 cgctgggtca ccggggagac ctggtatccg gccgaagcgg tgaagcagat gctgcccgcc
     7681 ttctccatcg accacgctca tccatcgtgg ccgaccaatc tgtggctgac ggcgttgctc
     7741 aagctgttcc ggccgcacat cgagtccttg atcgagcatc gcgaccgggt gatcgaggct
     7801 tggcagacca accacccagg ccaggacgta ttcgaggacc gggcgctgga gatcaccggc
     7861 tacctgccca tttcggtcga agagtggagc caggccctgg cacacgctca agccacgccg
     7921 ccccgagcca gatcatgatg gccaggaaca agacatgaag atccttttcc tctgtaccgc
     7981 caacagctgc cgcagcatcc ttgcggaagc catcttcaac caccaggcgc cggccggcct
     8041 gcgggccaac ctgccggatt gcgcggtgaa ggcatcggcc tgtatctacg ctgaagtcag
     8101 cacgctccgg agaagttgga atggaagaag aacgggagct ggtcaggcga ctgcagcaag
     8161 gggatccagc ggcctgcgac gcggtcatcc gggcccatca ccgctttctg gtggccatgg
     8221 ccgtgcccct ggtgggacag gggctggccg acgatgtcgc ccaggaaacc tggctgaagg
     8281 cctttgtagc gatcaagggc ttcgagggac gctcgcggct acgcacctgg ctggcccgca
     8341 tcgcgctcaa cgaggcgcac gcactgctgc gcaggtacaa gcgcgaagtg tcgctggagg
     8401 gctggggagc ggatagtggt tcgccgatcg cctccagctt cagcgaccag ggagcctgga
     8461 gtcacccgcc tgcgcaatgg cactacgaca cgcctgaagc cctgctgacc gaagccgagc
     8521 tgcacgagtg catccgcaag catctgcatc gcctgcccag cgaccagcaa ggtgtcgtgt
     8581 tgatgcgtga gctgggcggt ctcgagttcg ccgaaatcgc cgaggcaacc ggtctcagcg
     8641 aaggcaacat ccgtgtcctt ctgcatcggg ggcgacaacg catgcacgcc atgctcaacc
     8701 acttcgagga ggtgggcaca tgttgacctg caaggaaatg tcggagctgg gctcggacat
     8761 catcgatgac cacctggggc tacgcacgcg catggatgtc ttcatgcatc taggcatgtg
     8821 tccgcgttgc cggcgctata tcaagcagct
//
LOCUS       DATCUN010000079         2749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_59341, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000079 DATCUN010000000
VERSION     DATCUN010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59341"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VIO83_03255"
     CDS             <1..441
                     /locus_tag="VIO83_03255"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY8330330.1"
                     /translation="AAGAHGAPYGSGEPCVLGADTAVVLDGRILGKPQDRGDGLAMLA
                     ALSGRNHEVLTAVAVSDGTRCAARVVSSRVHFRALAEAEQQAYWDTGEPLDKAGGYAI
                     QGLAAIFVDRLEGSYSAVVGLPLAETAALLAEFGVACWQPEARP"
     gene            483..1940
                     /gene="rng"
                     /locus_tag="VIO83_03260"
     CDS             483..1940
                     /gene="rng"
                     /locus_tag="VIO83_03260"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEY8330331.1"
                     /translation="MSEEILINITPMESRVAVVENGVLQEVHVERTQKRGIVGNIYKG
                     KVVRVLPGMQAAFIDIGLERAAFIHASEISTREGSAVENISALVHEGQSLVVQVTKDP
                     IGSKGARLTTQLSIPSRYLVYMPRTRHVGISLKIEGEGERERLKQVVAECVAAEGIEN
                     QGGFILRTAAEGAGADEILADIRYLRRLWEQIGTQMQSAPAPSVIYEDLSLALRTLRD
                     LVGPRTEKIRIDSRETFQKVGLFVGELMPEVSDRLEHYPGERPIFDLYGVEDEIQKAL
                     ERKVLLKSGGYLIVDPAEAMTTIDVNTGAFVGHRNLEETIFKTNLEAATSIARQLRLR
                     NLGGIIIIDFIDMEDEEHRRQVLRTLEKMLERDHAKTNIIGITELGLVQMTRKRTRES
                     LQQVLCEPCASCQGRGQLKTAETICYEIFREILREARAYQAGSYLVLANQVVIERLLD
                     EESGNVADLEAFIGRPLRFQVEAKYSQELYDVVPL"
     gene            1955..>2749
                     /locus_tag="VIO83_03265"
     CDS             1955..>2749
                     /locus_tag="VIO83_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02099 family protein"
                     /protein_id="HEY8330332.1"
                     /translation="MAASGRLLARVLDGLFGLAALLLLLTALYVSLGRLLVPWVAEYR
                     AELQGRASALLGQPLHIGALEGRWRGFAPQLELRDLQLEADGEVLHLDRLRLVPDVFA
                     SLRTRSLRIASLEVTGLQLGLRQEADGSWRAKGLARPAEPRAALDPARILALLLAPGQ
                     LALLDSRLSIETRTGELLGFNYVGLTLDNRRGAQRLDGRLLLPDGQPLSWQLQGELRA
                     ETWREARAQLYLSLPQSEWGRWLGQLLPGSWQVERLQAGGEVWLEWA"
BASE COUNT          440 a          902 c          986 g          421 t
ORIGIN      
        1 gcggccggtg cgcatggcgc accctacggc tcgggcgagc cctgcgtgct gggcgccgat
       61 accgcggtgg tgctcgacgg gcgcattctc ggcaagccgc aggatcgtgg cgacggcctg
      121 gccatgctcg ccgccctgtc cgggcgcaat cacgaggtgc ttaccgcggt ggcggtgagc
      181 gacggcacgc gctgcgcggc gcgggtggtg agcagtcgtg tgcacttccg cgcgcttgcc
      241 gaggccgaac aacaggcata ctgggatacc ggcgaaccgc tggacaaggc tggtggctat
      301 gccatccagg gcctggcggc gattttcgtc gaccgcctgg agggcagcta ttctgccgtg
      361 gtcggtctgc cgctggccga aaccgccgcg ctgctggccg agttcggcgt ggcctgctgg
      421 cagcctgagg cgcgcccctg acgggcgacg catggacgat cccccgcgac gagaatgacg
      481 agatgagcga agaaatcctg atcaacatca cccccatgga gtcgcgcgtg gcggtggtgg
      541 aaaacggcgt gctgcaggag gtgcatgtcg agcgcacgca gaagcgcggc atcgtcggca
      601 atatctacaa gggcaaggtg gtgcgcgtgc tgcccggcat gcaggcggcg ttcatcgaca
      661 tcggcctgga gcgcgcggcc ttcatccatg cctcggaaat ctccacccgc gaaggcagtg
      721 cggtggagaa catcagcgcg ctggtgcacg aggggcagag cctggtggtg caggtgacca
      781 aggatccgat cggcagcaag ggcgcgcggc tgaccaccca gctgtcgatc ccctcgcgct
      841 atctggtgta catgccgcgc acccgccacg tcggcatctc gctgaagatc gagggcgagg
      901 gcgagcgcga gcggctcaaa caggtggtgg ccgagtgcgt ggccgccgag ggcatcgaga
      961 atcagggtgg cttcatcctg cgcaccgccg ccgaaggtgc cggcgccgac gagatcctcg
     1021 ccgacatccg ctacctgcgc cgcctgtggg agcagatcgg cacgcagatg cagagcgcac
     1081 cggcgccctc ggtgatctac gaggacctgt cgctggccct gcgcaccctg cgcgatctgg
     1141 tcggcccgcg caccgagaag atccgcatcg attcgcggga aaccttccag aaggtcgggc
     1201 tgttcgtcgg cgaactgatg ccggaagtct ccgaccgcct cgagcattac cccggcgagc
     1261 ggccgatctt cgacctgtac ggggtcgagg acgagatcca gaaggcgctg gaacgcaagg
     1321 tgctgctgaa gtccggcggc tacctgatcg tcgatccggc cgaggcgatg accaccatcg
     1381 acgtcaacac cggcgccttc gtcggtcatc gcaacctcga ggaaaccatc ttcaagacca
     1441 acctcgaggc cgccacctcg attgcccgcc agctgcgcct gcgcaacctg ggcgggatca
     1501 tcatcatcga cttcatcgac atggaggacg aggagcaccg gcgccaggtg ctgcgcaccc
     1561 tggagaagat gctcgagcgc gaccacgcca agaccaacat catcggcatc accgagcttg
     1621 ggctggtgca gatgacccgc aagcgtaccc gcgagagcct gcagcaggtg ctctgcgaac
     1681 cgtgtgcgag ctgccagggg cgcggccagc tgaagaccgc ggaaaccatc tgctacgaga
     1741 tcttccgcga gatcctccgc gaggcgcgcg cctaccaggc cggctcctat ctggtgctgg
     1801 ccaaccaggt ggtgatcgag cgtctgctgg acgaggagtc gggcaacgtc gccgatctgg
     1861 aggccttcat cggccgtccg ctgcgcttcc aggtggaggc caagtactcc caggaactct
     1921 atgatgtggt gccgctctga ggtcggaggc tgacatggcc gcctccggcc ggctgctcgc
     1981 ccgcgtcctg gatgggctgt tcggactggc ggccctgttg ctgctgctga ccgccctgta
     2041 cgtcagcctc gggcggctgc tggtgccgtg ggtggccgaa tatcgcgccg agctgcaagg
     2101 ccgcgccagt gcgctgctcg gccagccgct gcacatcggg gcgctggaag ggcgctggcg
     2161 gggcttcgcg ccgcaactgg agctgcgcga cctgcagctg gaggcggatg gcgaggtgct
     2221 gcatctggac cggctgcgcc tggtgccgga cgtcttcgcc agcctgcgca cgcgcagcct
     2281 gcgcatcgcc agcctggaag tgacggggct gcagctgggc ctgcgccagg aggcggatgg
     2341 cagctggcgc gccaaggggc tggcgcgccc cgcggaaccg cgtgcagcgc tcgatccggc
     2401 gcgcatcctc gcgctgctgc tggccccggg tcagctggcg ctgctcgaca gccgcctgag
     2461 catcgagacg cgtacgggcg aattgctcgg cttcaactat gtcggtctga ccctcgacaa
     2521 tcggcgcggc gcccagcgtc tggatggtcg cctgctgctg ccggacggcc agccgctgag
     2581 ctggcagctg cagggcgaac tgcgcgccga gacatggcgg gaggcgcggg cgcagctcta
     2641 tctcagcctg ccgcagagcg agtgggggcg ctggctgggc cagctactgc ccggaagctg
     2701 gcaggtcgaa cgcctgcagg ccggcggcga ggtctggctg gagtgggcc
//
LOCUS       DATCUN010000080         8220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_247764, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000080 DATCUN010000000
VERSION     DATCUN010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247764"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VIO83_03270"
     CDS             complement(<1..558)
                     /locus_tag="VIO83_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8330333.1"
                     /translation="MNLPSYTRGAQDQPLLAMTIGAAFDQAVGRFAEREALVVRDQGL
                     RYSWAELAAAVDRCARALLAIGLQPGERLGIWSTNRAEWCITQFASAKVGVVLVNVNP
                     AYQLSELEYALKQSGCRWLICAETFKTSDYHAMLAELLPELAGSAIGALHSHRLPELR
                     GVISLGAQPADGMLGWQPLQAMAAQV"
     gene            complement(974..1978)
                     /locus_tag="VIO83_03275"
     CDS             complement(974..1978)
                     /locus_tag="VIO83_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8330334.1"
                     /translation="MSSLESIQVATAMHNVEQQRQRAALWRKRGFCLALLGVALVAPL
                     AVYPVFLMKLLCFALFACAFNLLLGFAGLLSFGHAAFLASGGYVTGYLLSQYSGLSTE
                     LGILAGTAASGVLGLLFGLLAIRRQGIYFAMITLALAQLVFFMFVQAPFTGGENGLQG
                     VPRGHLFGLIDLSSNLAMYYFVLAVFVFGFAVIQRTIHSPYGQVLKAIRENEPRAISL
                     GYNVDAHKLLAFVISATLTGLAGATKTVVFQLASLTDAHWHMSGEVILMTLLGGVGTV
                     LGPLVGAGVVVTLQSYLSSGPLGDWVHVILGLIFVLCVLLFRAGIVGWVHKLIRRNFK
                     "
     gene            complement(1981..2868)
                     /locus_tag="VIO83_03280"
     CDS             complement(1981..2868)
                     /locus_tag="VIO83_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8330335.1"
                     /translation="MTLLFGIPLSVMLGQLLLGLINGAFYALLSLGLAIIFGLLRIIN
                     FAHGAQYMLGAFAAFLGLNYLGVNYWAALLLSPLLIGAIGMAVERYLLRRIAGEDHLY
                     GLLLTFGLALIVEGSFVKLFGVSGASFPMPELLKGGYNLGFMFLPTYRAWVVVAALLV
                     CIVTWYMIERTRLGSYLRAGTENPKLMQAFGINVPLLVTLTYGYGVALAAFAGVLAAP
                     IYPVSPTMGANLLIVVFAVVVIGGMGSIMGAIVTGLAMGLIEGLTKVFYPQAASTVVF
                     LVMVIVLLFRPAGLFGKEA"
     gene            complement(2985..4208)
                     /locus_tag="VIO83_03285"
     CDS             complement(2985..4208)
                     /locus_tag="VIO83_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8330336.1"
                     /translation="MKEFQKTATAILVSSLIAGTAQAQVSDDEIRIGYLADMSGTYRD
                     LAGPGGLEALKMAVEDFGGTVDGKKIVIFNADDLNKPDVGANTVRQWVDERNVDMVTG
                     LVATSVVLAASKVVEQADKLALISGAASSGLTNEYCSPNHIHWTYDTYALANGTAKAV
                     LAGGGKSWYMLTADYAFGHSMEADISKVVKEGGGSVLGSVRHPFPSNDFSSYVLQAQG
                     SGADVIALANAGADTVNALKTASQFGVTQAGQKLAGMVVFLNDIHAMGLDVTQGLMLT
                     TGWYWDMNDETRAWAKRYQARVGSMPTMAQAGVYSATTHYLNAVKATGSDATKTVRAQ
                     MASTPVNDMFAKNGQIRADGRMVHDMYLAQVKTPAESKGEWDLYRIVSTIPGEQAYRP
                     LAESQCKLVTKVANN"
     gene            complement(4318..5043)
                     /locus_tag="VIO83_03290"
     CDS             complement(4318..5043)
                     /locus_tag="VIO83_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8330337.1"
                     /translation="MTSLHTTPDFEQLRISDLHAFYGESHILHGIDLQVRRGELVTLL
                     GRNGSGRSTTLRAIMNLVGRRTGSIMINGNETIGVAAHLIPRLGVGYCPEERGIFASL
                     NVEENLLLPPTVRSGGMSLDEIYAMFPNLYERRFSQGTRLSGGEQQMLAMARILRTGA
                     NLLLLDEITEGLAPVIVQKLGEVLLKLKDKGLTIVLVEQNFRFAAPLADRHYLMEHGQ
                     IIEEIAADELAAKQELLHTCLGV"
     gene            complement(5040..5816)
                     /locus_tag="VIO83_03295"
     CDS             complement(5040..5816)
                     /locus_tag="VIO83_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8330338.1"
                     /translation="MAPEFVLETRGLTKEFRGFTAVDSVNLRVQKGHIHALIGPNGAG
                     KTTVFNLLTKFLSPTRGEILYFGKPITAMKPNEIARLGLVRSFQISAVFGHMSVRDNV
                     RVALQQREGNSFHFWKSARSLDALNARADQLLAEVDLLGFADTLTIELPYGRKRALEL
                     ATTLALDPKVLLLDEPTQGMGHEDVDMVVGLVRRAAVGRTVLMVEHNLSVVSKLCDRI
                     TVLARGAILAEGDYATVSANPQVREAYLGSEAAALEEAHA"
     gene            complement(5942..7216)
                     /locus_tag="VIO83_03300"
     CDS             complement(5942..7216)
                     /locus_tag="VIO83_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015802 - basic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family porin"
                     /protein_id="HEY8330339.1"
                     /translation="MHVKPSTPLFLLTGLAAALGASAPAAAAFVEDSHLSLDARNFYM
                     NRDYRDADKADTPRHKGAAKSKAEDWGQGFMLRFESGFTEGTLGFGVDALGLLGLQLD
                     SGGGTSGTGALLRNPRTGEAADEFSFVGPTAKARLAQSVLTVGTHTPLLPVAYRNDTR
                     MLPQTFEGAQLVSKDIDKLTLTGGQFRSTRLRDSTDYQDMTMFADGARGGVKSDRFNY
                     AGASYAPLANLTASYFYAELEDNYHQHYANLLHSLPLSDRLKLKTDLRYFDSGDEGNT
                     TVDNRNLGGMFTLVYQGHSLGFAYQDQRGDTGMPFIGGGTDPFTLNTLTYHHFLRAEE
                     DSWQARYDYDFAALGVPGLTLMGRYVRGDDFQIRGADANEWERDIDLSYVVQSGPLKN
                     VGLRWRNVAYRGSHTTDIDENRLIVNYTFKFW"
     gene            complement(7330..7923)
                     /locus_tag="VIO83_03305"
     CDS             complement(7330..7923)
                     /locus_tag="VIO83_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339645.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEY8330340.1"
                     /translation="MTAPISRFPVPESLDALPADLCERILAVQDRAGFVPNVFLMLAH
                     RPDEFRAFFAWHDALMERESDSLTLAEKEMIVVATSARHGCLYCVVAHGAVLRIYSKQ
                     PHLADQLAINHRTAAIGERQRVMIDFAMHLSFAHGVLDDAWQARLQGVGFSLNDIWDI
                     GAVAAFFSLSNRLVSLAGTPPNDEFYLMGRVPRAPRA"
BASE COUNT         1315 a         2624 c         2927 g         1354 t
ORIGIN      
        1 gacctgcgcg gccatggcct gcagcggctg ccagccgagc atgccgtcgg ccggctgcgc
       61 gccgaggctg atcaccccgc gcagctccgg cagcctgtgg ctgtgcagcg cgccgatggc
      121 gctgccggcc agctccggca gcagctcggc gagcatggcg tggtagtcgg aggtcttgaa
      181 ggtctcggcg cagatcagcc agcggcagcc ggactgcttg agcgcgtact caagttcgct
      241 cagctggtag gcggggttga cgttgaccag caccacgccg accttggcgc tggcgaactg
      301 ggtgatgcac cactcggcac ggttggtcga ccagatgccg aggcgctcgc ccggctgcag
      361 gccgatggcc agcagagcgc gggcgcagcg gtcgaccgcc gcggccagct cggcccagct
      421 gtagcgcagg ccctggtcgc gcaccaccag cgcctcgcgc tcggcaaaac ggccgaccgc
      481 ctgatcgaag gcggcgccga tggtcatggc cagcagcggc tggtcctgag cgccgcgggt
      541 gtagcttgga aggttcatgg agtccctcgg tcttgtgctt tttatgaggt ttgccagggc
      601 aaaatcctgc gcttgccgcg ccggcttggc gagcggaccg tcttgccagg cgggttcacg
      661 gcaacgccag gcggatgccg ctcgactgca aacccggtgg aaagtaccac cggcagcccc
      721 tgcgcggcac tccgcggctc gggagcggtc gcctgcggca tggcgccacc agcatgccga
      781 aggaccggca cgccggtcgc cgccgcaggt cgcaaagcgc agcaatacct gacggcacaa
      841 gacggactct ctgacggacc cggacctccg ctcgctgccg gcgccggatc agcgctgcag
      901 cgcagcgaaa cgcgtccggc gcgcccgatt cagctcggga agcgcgccga acgcccgcgc
      961 ggcccgggtc gcctcacttg aagttgcggc ggatcagctt gtgcacccag ccgacgatgc
     1021 cggcgcggaa cagcagcacg cagagcacga agatcaggcc gaggatcacg tgcacccagt
     1081 cgcccagcgg gccgctggac aggtagctct gcagggtcac caccacgccg gcgccgacca
     1141 gcgggccgag cacggtgccg acgccgccga gcagggtcat caggatcacc tcgccggaca
     1201 tgtgccagtg cgcgtcggtc agcgaggcca gctggaacac cacggtcttg gtcgccccgg
     1261 ccaggccggt gagggtcgcc gagatgacga aggccagcag cttgtgggcg tcgacgttgt
     1321 agcccagcga gatcgcccgc ggctcgttct cgcggatcgc cttgagcacc tggccgtagg
     1381 gcgagtggat ggtgcgctgg atcaccgcga agccgaacac gaacaccgcc agcacgaagt
     1441 agtacatcgc caggttgctc gacaggtcga tcaggccgaa caggtggcca cgcggcacgc
     1501 cctgcaggcc gttctcgccg ccggtgaacg gcgcctgcac gaacatgaag aacaccagct
     1561 gggccagcgc cagggtgatc atggcgaagt agatgccctg gcgacggatc gccaagaggc
     1621 cgaacagcag gccgagcacc ccggaagccg cggtgccggc gaggatgccc agctcggtgg
     1681 acagtcccga gtactggctg agcaggtagc cggtgacgta gccgccgctg gcgaggaacg
     1741 ccgcgtggcc gaaggacagc agccccgcga agccgagcag caggttgaag gcacaggcga
     1801 acagggcgaa gcacagcagc ttcatcagga acaccgggta caccgccagc ggtgcgacca
     1861 gcgccacgcc gagcagtgcc aggcagaaac cgcgcttgcg ccacagcgcg gcgcgctggc
     1921 gttgctgctc gacgttgtgc atggccgtag ccacttgaat gctttccaga ctcgacatgc
     1981 tcatgcctcc ttcccgaaca gtcccgcagg acggaacagc aggacgatca ccatcaccag
     2041 gaacaccacg gtgctggccg cctgcggata gaacaccttg gtcaggccct cgatcaggcc
     2101 catggccaga ccggtgacga tcgcgcccat gatcgagccc atgccgccga tcaccaccac
     2161 ggcgaacacc acgatcagca ggttggcgcc catggtcggg ctgaccgggt agatcggcgc
     2221 cgccagcacg ccggcgaacg ccgccagggc cacgccgtag ccgtaggtca gggtgaccag
     2281 cagcggcacg ttgatgccga aggcctgcat cagcttggga ttctcggtgc cggcgcgcag
     2341 gtaggagccc aggcgggtgc gctcgatcat gtaccaggtg acgatgcaca ccagcagcgc
     2401 cgccaccacc acccaggcgc ggtaggtggg caggaacatg aagccgaggt tgtagccgcc
     2461 cttgagcagc tcgggcatcg ggaaggaggc gccggacacg ccgaacagct tgacgaagct
     2521 gccctcgacg atcagcgcca ggccgaaggt cagcagcaga ccgtacaggt ggtcctcgcc
     2581 ggcgatgcgc cgcagcaggt agcgttcgac ggccatgccg atggcaccga tcagcagcgg
     2641 cgacagcagc agcgcggccc agtagttgac gcccaggtag ttgaggccga ggaaggcggc
     2701 gaaggcgccc agcatgtact gggcgccatg ggcgaagttg atgatgcgca gcaggccgaa
     2761 gatgatcgct aggccgaggc tgagcagggc atagaaggcg ccgttgatca ggccgagcag
     2821 cagctggccg agcattacgc tcaggggaat accgaacagg agagtcatga ttcaccaccc
     2881 aggggaagtt cgcgacaccc ggccgtgaac ggccgggcgg atggcctggt ggtgcacggc
     2941 gacgcctgcg ctcggcgacg ttccgcgcac cgcccgtcag ccgatcagtt gttcgccacc
     3001 ttggtcacca gcttgcactg gctttccgcc agcggccggt aggcctgctc gccggggatg
     3061 gtgctgacga tcctgtacag gtcccactcg cccttggact cggccggggt cttcacctgg
     3121 gccaggtaca tgtcgtggac catgcggccg tcggcgcgga tctggccgtt cttggcgaac
     3181 atgtcgttga ccggggtgga ggccatctgc gcgcgcacgg tcttggtggc gtcgctgccg
     3241 gtggccttga ccgcgttcag gtagtgcgtg gtcgccgaat acacgccggc ctgggccatg
     3301 gtcggcatgc tgccgacgcg ggcctgataa cgcttggccc aggcgcgggt ctcgtcgttc
     3361 atgtcccagt accagccggt ggtcagcatc aggccctggg tcacgtccag gcccatggca
     3421 tggatgtcgt tgaggaacac caccatgccg gccagcttct gcccggcctg ggtgacgccg
     3481 aactggctgg cggtcttcag cgcgttgacc gtgtcggcgc cggcgttggc cagggcgatc
     3541 acgtcggcgc cggaaccctg cgcctgcagc acgtaggagg agaagtcgtt gctggggaac
     3601 gggtggcgca ccgaaccgag cacgctgccg ccaccctcct tgaccacctt gctgatgtcg
     3661 gcctccatgg agtgaccgaa ggcatagtcg gcggtcagca tgtaccagct cttgccgccg
     3721 ccggcgagca ccgccttggc ggtgccgttg gccagcgcgt aggtgtcgta ggtccagtgg
     3781 atgtggttgg gcgagcaata ctcgttggtc agcccggagg acgccgcgcc ggagatcagc
     3841 gccagcttgt cggcctgctc gaccaccttg ctggccgcca gcaccaccga ggtggccacc
     3901 aggccggtca ccatgtcgac gttgcgctcg tcgacccact ggcgcacggt gttggcgccg
     3961 acgtccggct tgttgaggtc gtcggcgttg aagatgacga tcttcttgcc gtccaccgtg
     4021 ccgccgaaat cctccaccgc catcttcagc gcctccaggc cgccggggcc ggcgaggtcg
     4081 cggtaggtgc cggacatgtc ggcgaggtag ccgatgcgga tttcgtcgtc gctgacctgg
     4141 gcctgggcag tgccggcaat caggctggag acgaggatgg cggttgcggt cttctggaat
     4201 tccttcatgt gttcacccac ttttccatgg ttattgttgt tgtgcgggcc gacggcagcc
     4261 cgtaccggca aggcgcaggg ccgcgccggc tgggacatgt cgcggggtac tacgggttca
     4321 gacgccgagg caggtgtgca gcagctcctg cttcgccgcc agttcgtcgg cggcgatctc
     4381 ctcgatgatc tggccgtgct ccatcaggta gtggcggtcg gccagcggcg cggcgaagcg
     4441 gaagttctgt tcgaccagga cgatggtcag gcccttgtcc ttgagcttga gcagcacctc
     4501 gccgagcttc tgcacgatca ccggggccag gccctcggtg atctcgtcga gcagcagcag
     4561 gttggcgccg gtgcgcagga tgcgcgccat ggccagcatc tgctgttcgc cgccggacag
     4621 ccgggtgccc tggctgaagc ggcgctcgta gaggttgggg aacatcgcgt agatctcatc
     4681 gaggctcatg ccgccgctgc gcacggtggg cggcagcagc aggttttcct cgacgttgag
     4741 gctggcgaag atgccgcgct cttccgggca gtagccgacg cccaggcgcg ggatcaggtg
     4801 ggcggcgaca ccgatggtct cgttgccgtt gatcatgatc gagccggtgc gccggccgac
     4861 cagattcatg atcgcacgca gggtggtgct gcgcccggag ccgttgcgcc cgagcagggt
     4921 caccagttcg ccgcggcgca cctgcaggtc gatgccatgc aggatgtgcg actcgccgta
     4981 gaaggcgtgc aggtcgctga tgcgcagctg ctcgaagtcc ggagtggtgt gcaggctggt
     5041 catgcgtggg cctcctcgag ggcggcggct tcgctgccca gataggcctc gcgcacctgc
     5101 gggttggccg acaccgtggc gtagtcgccc tcggcgagga tggcgccgcg cgccagcacg
     5161 gtgatgcggt cgcacagctt gctgaccacg ctgaggttgt gttccaccat cagcacggtg
     5221 cgcccgaccg cggcgcggcg caccaggccg accaccatgt cgacgtcctc gtggcccatg
     5281 ccctgggtcg gctcgtcgag cagcagcacc ttggggtcca gggccagggt ggtggccagc
     5341 tcgagggcgc gcttgcgccc gtagggcagc tcgatggtca gggtgtcggc gaagccgagc
     5401 aggtcgacct cggcgagcag ctggtcggcg cgcgcgttga gcgcatccag gctgcgcgcc
     5461 gatttccaga agtggaagga gttgccctcg cgctgctgca gggccacgcg cacgttgtcg
     5521 cgcacgctca tgtggccgaa caccgcggag atctggaacg agcgcaccag gcccaggcgg
     5581 gcgatctcgt tgggcttcat ggcggtgatc ggcttgccga agtagaggat ctcgccgcgg
     5641 gtggggctga ggaacttggt gagcaggttg aacaccgtgg tcttgccggc cccgttgggg
     5701 ccgatcagcg catggatgtg tcccttctgc acacgcaggt tgaccgaatc gaccgcggtg
     5761 aagccgcgga attccttggt caggccgcgt gtttccagca cgaactctgg tgccatgggg
     5821 tgccctctaa ccttgattgt ttttgttggt tatggcgcag gtcgctgaat gacctttacg
     5881 taaacgataa caacgcgtgt cagtgccgac aagccgtttc tgcacgcgtt gtcgggcgtg
     5941 cttaccagaa cttgaaggtg tagttgacga tcaggcggtt ctcgtcgata tcggtggtgt
     6001 ggctgccgcg ataggcgacg ttgcgccagc gcaggccgac gttcttcagc gggccgctct
     6061 gcaccacata gctgaggtcg atgtcgcgtt cccactcgtt ggcatcggcg ccgcggatct
     6121 ggaagtcgtc gccgcgcacg tagcggccca tcagggtcag gccgggtacg ccgagggcgg
     6181 cgaagtcgta gtcgtagcgg gcctgccagg agtcttcctc ggcgcgcagg aagtggtggt
     6241 aggtcagggt gttgagggtg aacggatcgg tgccgccgcc gatgaacggc atgccggtat
     6301 cgccgcgctg gtcctggtag gcgaagccca gcgagtggcc ctggtagacc agggtgaaca
     6361 tgccgccgag gttgcggttg tcgaccgtgg tgttgccttc gtcgccgctg tcgaagtagc
     6421 gcaggtcggt cttcagcttg aggcggtcgc tcagcggcag gctgtgcagc aggttggcgt
     6481 agtgctggtg gtagttgtct tccagctcgg catagaagta gctggcggtc aggttggcca
     6541 gcggcgcgta gctggcgccg gcgtagttga agcggtcgct cttcacgccg cccctggcgc
     6601 catcggcgaa catggtcatg tcctggtagt cggtggagtc gcgcaggcgg gtgctgcgga
     6661 actggccgcc ggtcagggtc agcttgtcga tgtccttgga caccagctgc gcgccctcga
     6721 aggtctgcgg cagcatgcgc gtgtcgttgc gataggccac cggcagcagc ggggtgtgcg
     6781 tgccgacggt gagcacggac tgggcaagcc gcgccttggc ggtcggtccg acgaagctga
     6841 actcgtcggc cgcctcgccg gtcctcgggt tgcgcagcag cgcgccggtg ccgctggtgc
     6901 cgccgccgga gtcgagctgg aggccgagca ggccgagggc atcgacgccg aagccgagcg
     6961 tgccttcggt gaagccggac tcgaagcgca gcatgaagcc ctgcccccag tcctccgcct
     7021 tgctcttggc cgcgcccttg tgcctgggcg tgtcggcctt gtccgcatcg cgatagtcgc
     7081 ggttcatgta gaagttgcgc gcgtcgaggc tcaggtggct gtcctcgacg aaggccgccg
     7141 cagccggcgc cgaagccccc agggcggccg ccaggccagt gagcaggaac aacggggtag
     7201 agggtttgac gtgcatatcg agtctctctt gttattgttg gactgttgat tcagcacttt
     7261 cagtgcccgg ccgcccgccg tcaggcgcga ccgggcttgc cgcaggcgtt gcggcgggac
     7321 accccgcaat caggcgcgcg gggctctggg cacgcggccc atcaggtaga actcgtcgtt
     7381 cggcggcgtg ccggccagcg acaccaggcg gttggacagg ctgaagaagg cggccacggc
     7441 gccgatgtcc cagatgtcgt tcaggctgaa accgacgcct tgcaggcgcg cctgccaggc
     7501 gtcatccagc acgccgtgcg cgaagctcag gtgcatggcg aagtcgatca tcacccgctg
     7561 gcgctcgccg atggccgcgg tgcggtggtt gatcgccagc tggtcggcga ggtgcggctg
     7621 cttgctgtag atgcgcagca ccgcgccatg cgccaccacg cagtacaggc agccgtggcg
     7681 tgcgctggtg gcgaccacga tcatctcctt ctcggccagg gtcaggctgt cggactcgcg
     7741 ctccatcagc gcgtcgtgcc aggcaaagaa ggcgcggaac tcgtcgggac ggtgcgccag
     7801 catgagaaac acgttgggca cgaagcccgc gcggtcctgc acggcgagga tgcgctcgca
     7861 caggtcggcc ggcagggcgt cgaggctttc gggaacggga aaacggctga tcggtgcggt
     7921 catggggatt cctttcttat tatccgattc attgatggta cccacgctcc cgcgtgggta
     7981 cccggcacag gggcgctcct gcgcccgtta gcacgctgga gggacgctga gcgtccgggc
     8041 ggcgttccgt cgggcggcgt tcccacgcgg agcgtgggaa cgatccccct ctcctgccgc
     8101 ctcagtgctg ggtgatcccc agctccagca cgctgatctc gcgcatgcgg aacttctgca
     8161 ccttgccgct gatggtcatc gggaagtcgt cgacgaactt gatgtgccgc ggcgtcttga
//
LOCUS       DATCUN010000081         5438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_134474, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000081 DATCUN010000000
VERSION     DATCUN010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134474"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            474..1340
                     /gene="purU"
                     /locus_tag="VIO83_03310"
     CDS             474..1340
                     /gene="purU"
                     /locus_tag="VIO83_03310"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017903948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEY8330341.1"
                     /translation="MQHAQNQYVIKISCPATSGIVASVTSFLADNGCYISEMAQFDDE
                     ISGTFFMRAVFRFNAGFTGDIEAIKQGFVDVAGKFDMHWELHDAAKPMRVLLMVSKFD
                     HCLSDLLYRHQKGELHMQITAVVSNHLDLRPMAEREGIRFIYLPVTKDTKAQQEAELM
                     KIVDETQTDLVVLARYMQILSNDLCKQLSGRAINIHHSFLPGFKGAKPYHQAFERGVK
                     LIGATAHYVTADLDEGPIIEQEVQRVDHNYAPDALVAVGRDTETVALSKAVKYHLEHR
                     VFLNHDKTVIFR"
     gene            1337..2257
                     /gene="folD"
                     /locus_tag="VIO83_03315"
     CDS             1337..2257
                     /gene="folD"
                     /locus_tag="VIO83_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEY8330342.1"
                     /translation="MNSAAPGQLIDGKAVSAQVLAEVTADVAALKAQGVEPALAVVLV
                     GDDPASHVYVRNKVLRAGEVGIRSLEYRLPAEASAAEVLAVVAELNGDAGVHGILVQL
                     PLPRHIDETAVLQAIDPAKDVDGFHSENVGGLSQGRPVLTPCTPAGCMRLLRDTLGDL
                     SGKHAVVIGRSNIVGKPMAALLLAADCTVTVLHSRSKNPQALCRKADIVVAAVGRPRM
                     INAEWIKPGAVVIDVGINRIEEDGKNRLVGDVDFASVQPLTAAITPVPGGVGPMTIAL
                     LMQNTVTAACQQHINSAPEASEEATCRSAC"
     gene            2239..3480
                     /locus_tag="VIO83_03320"
     CDS             2239..3480
                     /locus_tag="VIO83_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003173309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8330343.1"
                     /translation="MPFSLLKYGLSSDYPVDVDLPAPKELKSSYDVVIIGGGGHGLAT
                     AYYLAKYHGITNVAVLEKAYLGGGNTARNTAVIRSNYLTSEGVKFYAESVRLFKELSN
                     EFDFNIMYSERGQLTLAHTDATVRAFRQRAEVNKHFGGRTEMIDRQQIRELVPTLNLD
                     PGHLPVLAGLWHIDGATARHDAVAWGYAKQAAKRGVEIHQLTEVQDFVVENGRVTAVK
                     TNRGTVKCGCAVQAIAGHSSLLLNKLGIRAPIHSFPLQAMVTQPFKPFLDPLVSSSAL
                     HCYVQQTGRGEVVFGGGSDPYPLYNTRSTLDLKESLLAHAIEMFPFLANAKLMRQWAG
                     TTDMTPDYSPIMGRSPVDNYYLDAGWGTWGFKSTPICGKTMAELVASGGRTPELIKPF
                     ALERFSTFQQVNEMGATAASH"
     gene            3491..3799
                     /locus_tag="VIO83_03325"
     CDS             3491..3799
                     /locus_tag="VIO83_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003118328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEY8330344.1"
                     /translation="MKIMPCPLNGPRNISEFTYGGEFKPMPDPATCSDADWADYVFNS
                     DNRAGLVVEWWMHNASSYWFLAERHTVTDEIVRTFDPKEIFTQRVDFAAPAKPQEVVA
                     "
     gene            3796..>5438
                     /locus_tag="VIO83_03330"
     CDS             3796..>5438
                     /locus_tag="VIO83_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011267520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8330345.1"
                     /translation="MTAYSRLPAPMGLLIDRDRPLAFNFDGKPYQGFQGDSIASALLA
                     NGRWLLSRSFKYHRPRGPLTMAGQDANTLVQLPSEPNVLADLQPLAEGLTVTGQNFAG
                     SLDNDRDALLGKFSKFMPVGFYYRSFYKPMGIWKVWEPIIRKKAGLGVLDLKFQPEYH
                     DKAFLFVDLAVVGAGPAGLTAALHAANAGAKVLLVEQQSYLGGALAWARFDIEGKRAD
                     ALRRELVDAVQGHPNIQILTEATCNAWFTDNFLPVIQGNRLYKVRAKQCIVAAGAFDQ
                     PVVFRNNDLPGVMLASAAQRLIKLYAVKPGQRAVVLTGNDDGYLAALDLFEAGVDVAA
                     VVDMRTQPADADLAMALKKAGIPLHSGSTVYEALHAKGMRHITGVDVRRIVGKGQVAG
                     KGMQLDCDLLCMSAGYMPVYQLLCQAGGKLSYDDAQAAFALSGLPKNLTVAGSVNGRH
                     ALDNVLREAAHVASAACASLGLQAQAAPASFVAEPRVNFDWPIFPHPDGKDFVDFDED
                     LQVRDIVNATRHGYRDVQLVKRFSTVGMGPSQGRHSALPT"
BASE COUNT          984 a         1962 c         1674 g          818 t
ORIGIN      
        1 atcctgcgcg ggccggtgtg gaacagcatg ggctcttcat ccaccaccag gtactggttg
       61 aacgagaagc cgccaccttc cagcgccacc ggcgtgttga tacggtagat gccgtcggcg
      121 atctcgacga cgttggtgcc cgactggctg ttggtgatgg tcatggtgca ctcccgccca
      181 ggacgtcccc tgacagttaa gcagcctggc cgctcaaggc ccgagcgctg tccccccctg
      241 tccacgcgct gcacgccaac cgggcgccgc ccgcgcaggc aatgaccgcc aaaaaattcc
      301 ccacaagata aaaaatttca ccacaggcat ggacacggcg attttgttcg cttataaaaa
      361 caccagcacg aaaattttat tcccagcagt caaaaatatt caggaatgaa atcggctttc
      421 accgacgaat ttttcgcctt gcaaccgctg cctccacctc cagaggactc gacatgcaac
      481 acgctcagaa ccagtacgtc atcaagatca gctgcccggc cacttccggc atcgtcgcca
      541 gcgtgacctc cttcctcgcc gacaacggct gctacatcag cgagatggcg cagttcgacg
      601 acgagatcag cggcaccttc ttcatgcgcg cggtgttccg tttcaatgcc ggcttcaccg
      661 gcgatatcga ggcgatcaag cagggcttcg tcgatgtcgc cggcaagttc gacatgcact
      721 gggaactgca cgacgccgcc aagccgatgc gcgtgctgct gatggtcagc aagttcgacc
      781 actgcctgtc cgacctgctc taccgccacc agaagggcga gctgcacatg cagatcaccg
      841 ccgtggtttc caaccacctc gacctgcggc cgatggccga gcgcgagggc atccgcttca
      901 tctacctgcc ggtgaccaag gacaccaagg cccagcagga agccgagctg atgaagatcg
      961 tcgacgagac ccagaccgac ctggtggtgc tggcgcgcta catgcagatc ctctccaacg
     1021 atctgtgcaa gcaactttcg ggccgtgcca tcaacatcca ccactcgttc ctgcccggct
     1081 tcaagggcgc caagccctac caccaggcct tcgagcgcgg cgtgaagctg atcggcgcta
     1141 ctgcccacta cgtgaccgcc gacctcgacg agggcccgat catcgagcag gaagtgcagc
     1201 gcgtggacca taactacgca ccggacgcgc tggtcgccgt cggccgcgac accgaaaccg
     1261 tggccctttc caaggcggtc aagtaccacc tggagcaccg cgtgttcctc aaccacgaca
     1321 agacggtgat cttccgatga acagtgcagc tcccggccaa ctgatcgacg gcaaggccgt
     1381 ctccgcccag gtcctcgccg aagtgaccgc cgatgtcgcc gcgttgaagg cgcaaggcgt
     1441 cgagccggcg ctggcggtgg tgctggtcgg cgacgacccg gccagccacg tctacgtgcg
     1501 caacaaggtg ctgcgcgccg gcgaggtggg tatccgctcc ctcgagtacc gcctgccggc
     1561 cgaggccagc gctgccgagg tgctggcagt ggtcgccgag ctgaatggcg atgccggcgt
     1621 gcacggcatc ctggtgcagc tgccgctgcc caggcacatc gacgagaccg cggtgctgca
     1681 ggccatcgac ccggccaagg acgttgacgg cttccacagc gagaacgtcg gcggcctcag
     1741 ccagggccgc ccggtgctca ccccgtgcac cccggccggc tgcatgcgcc tgctgcgcga
     1801 caccctcggc gacctgtccg gcaagcacgc ggtagtgatc ggccgctcca acatcgtcgg
     1861 caagccgatg gccgccctgc tgctcgccgc cgactgcacg gtgaccgtgc tgcactcgcg
     1921 cagcaagaac ccgcaggcgc tgtgccgcaa ggccgacatc gtggtcgccg cggtcggccg
     1981 gccgcggatg atcaacgccg agtggatcaa gcccggcgcg gtggtgatcg acgtcggcat
     2041 caaccgcatc gaggaagacg gcaagaaccg cctggtcggc gacgtcgact tcgccagcgt
     2101 gcagccgctg accgcggcga tcaccccggt gcccggcggc gtcgggccga tgaccatcgc
     2161 cctgctgatg cagaacaccg tgaccgccgc gtgccagcaa cacatcaact cggcccccga
     2221 ggccagtgag gaggccacat gccgttcagc ctgctgaaat acggcctgtc ttcggactac
     2281 ccggtggacg tcgatctgcc ggcgccgaag gagctgaagt cgtcctacga cgtggtgatc
     2341 atcggcggcg gcggccacgg cctggccacc gcctactacc tggccaagta ccacggcatc
     2401 accaacgtgg cggtgctgga gaaggcctac ctgggcggcg gcaacaccgc gcgcaacacc
     2461 gcggtgatcc gctccaacta cctcaccagc gagggcgtga agttctacgc cgagtcggtg
     2521 cggctgttca aggagctgtc caacgagttc gacttcaaca tcatgtactc cgagcgcggc
     2581 cagctgaccc tggcgcacac cgatgccacg gtgcgcgcct tccgtcagcg cgccgaagtg
     2641 aacaagcact tcggcgggcg taccgagatg atcgaccgcc agcagatccg cgagctggtg
     2701 ccgacgctga acctcgaccc gggccacctg ccggtgctcg ccgggctgtg gcacatcgac
     2761 ggcgccaccg cgcgccacga tgcggtggcc tggggctacg ccaagcaggc ggccaagcgc
     2821 ggggtggaga tccaccagct caccgaggtg caggacttcg tggtggagaa cggccgggtc
     2881 acggcggtga agaccaaccg cggcaccgtc aagtgcggct gcgcggtgca ggcgatcgcc
     2941 gggcacagct cgctgctgct caacaagctg ggcatccgcg cgccgatcca cagcttcccg
     3001 ctgcaggcga tggtcaccca gccgttcaag ccgttcctcg acccgctggt cagctcgtcg
     3061 gcgctgcact gctacgtgca gcagaccggc cgcggcgagg tggtgttcgg cggcggctcc
     3121 gacccctacc cgctgtacaa cacccgctcg accctcgacc tcaaggaaag cctgctggcc
     3181 cacgccatcg agatgttccc cttcctggcc aacgccaagc tgatgcgcca gtgggcgggg
     3241 accaccgaca tgaccccgga ctacagcccg atcatgggcc gctcgccggt ggacaactac
     3301 tacctcgacg ccggctgggg cacctggggc ttcaagtcga cgccgatctg cggcaagacc
     3361 atggccgaac tggtagccag cggcggcagg acgccggagc tgatcaagcc gttcgccctg
     3421 gagcgtttct cgaccttcca gcaggtcaac gaaatgggcg ccaccgccgc cagtcactga
     3481 ggaatccgcg atgaagatca tgccctgccc cctgaacggt ccccgcaaca tcagcgaatt
     3541 cacctacggc ggcgagttca agccgatgcc cgacccggcc acctgcagcg acgccgactg
     3601 ggccgactac gtgttcaaca gcgacaaccg cgccggcctg gtggtcgagt ggtggatgca
     3661 caacgcctcc agctactggt tcctcgccga acgccacacc gtcaccgacg agatcgtgcg
     3721 caccttcgat cccaaggaaa tcttcaccca gcgcgtcgac ttcgccgcgc ccgccaagcc
     3781 ccaggaggtc gtcgcatgac cgcttacagc cgcctgcccg cacccatggg cctgctgatc
     3841 gaccgcgatc ggccgctcgc tttcaacttc gacggcaagc cgtaccaggg cttccagggc
     3901 gacagcatcg ccagcgccct gctcgccaac ggccgctggc tgctgtcgcg ctcgttcaag
     3961 taccaccgcc cgcgcggccc gctgaccatg gccggccagg acgccaacac cctggtgcag
     4021 ctgcccagcg agcccaacgt gctggccgac ctgcagccgc tggccgaggg cctgaccgtc
     4081 accgggcaga acttcgccgg ctcgctggac aacgaccgcg acgccctgct cggcaagttc
     4141 tccaagttca tgccggtcgg cttctactac cgctccttct acaagccgat gggcatctgg
     4201 aaagtgtggg agccgatcat ccgcaagaag gccggcctcg gcgtgctgga cctgaagttc
     4261 cagcccgagt accacgacaa ggccttcctg ttcgtcgacc tcgccgtggt cggcgccggc
     4321 ccggccggcc tcaccgccgc gctgcatgcc gccaacgccg gcgccaaggt gctgctggtc
     4381 gaacagcagt cgtacctggg cggcgccctg gcctgggcgc gcttcgacat cgaaggcaag
     4441 cgcgccgacg cgctgcgccg cgagctggtc gacgcggtgc aaggccaccc gaacatccag
     4501 atcctcaccg aggccacctg caacgcctgg ttcaccgaca acttcctgcc ggtaatccag
     4561 ggcaaccgtc tgtacaaggt gcgcgccaag cagtgcatcg tcgccgccgg cgccttcgac
     4621 cagccggtgg tgttccgcaa caacgacctg ccaggcgtga tgctggccag cgcggcgcag
     4681 cggctgatca agctgtacgc ggtcaaaccg ggccagcgcg cggtggtgct gaccggcaac
     4741 gacgacggct acctggccgc cctcgacctg ttcgaggcgg gcgtggacgt cgccgcggtg
     4801 gtcgacatgc gcacccagcc ggccgatgcc gacctcgcca tggcgctgaa gaaggccggc
     4861 attcctctgc acagcggcag caccgtgtac gaggcgctgc acgccaaggg catgcgccac
     4921 atcaccggcg tcgacgtgcg gcgcatcgtc ggcaagggcc aggtggccgg caagggcatg
     4981 caactcgact gcgacctgct gtgcatgtcc gcgggctaca tgccggtcta ccagctgctc
     5041 tgccaggccg gcggcaagct gagctacgac gatgcccagg ccgccttcgc cctcagcggc
     5101 ctacccaaga atctgacggt ggccggttcg gtcaacggtc gccacgccct cgacaacgtg
     5161 ctgcgcgaag ccgcccatgt cgccagcgcc gcctgcgcca gcctcggcct gcaggcgcaa
     5221 gcggcaccgg ccagcttcgt cgccgaaccg cgggtcaact tcgactggcc gatcttcccg
     5281 cacccggacg gcaaggactt cgtcgacttc gacgaggacc tgcaggtgcg cgacatcgtc
     5341 aacgccaccc gccacggcta ccgcgacgtg cagctggtca agcgcttctc caccgtcggc
     5401 atgggcccgt cgcagggccg ccactcggcg ctgccgac
//
LOCUS       DATCUN010000082         1945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_78385, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000082 DATCUN010000000
VERSION     DATCUN010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78385"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VIO83_03335"
     CDS             <1..811
                     /locus_tag="VIO83_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OST-HTH/LOTUS domain-containing protein"
                     /protein_id="HEY8330346.1"
                     /translation="DVQRLLGRCLLRLQQYERLMKAIVAHHEISGPAHSLEAIRAARI
                     EDAATKTLGTLIGQLFGSYVVTDGNGGEERDDDLPGDVISFRTRVQLSLSAQDYAKTQ
                     ADLKDLVSLRNTLVHHFIDQHDLWTVDGCRAAQDELGSAYTRIDQHFEQLRGWAEHMD
                     QARRLAAEFVQSDVFHDLVVNGIAPDGTVDWPAAGIVRALREAAAQLAVEGWTPIAAA
                     GRWIADRHPEQLPAKYGCSSWRQVVHECRLFELRYREVEGQRAAWYRPREA"
     gene            999..1787
                     /locus_tag="VIO83_03340"
     CDS             999..1787
                     /locus_tag="VIO83_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03761 family integrating conjugative element
                     protein"
                     /protein_id="HEY8330347.1"
                     /translation="MANERIEPLQLNLGSLRSAMSLTLHTHHASRIWHGRAPTEGRPG
                     IIGLNGFIGAMNKMQRGAEQDDPYSDWWMLRIEDKLADTKTRLQTLREQVDQALADVP
                     AALSLGENMNMQPVKLPLFVNAQLGFMAVYLLADYDDLARKLILAHHTALIDRSTLER
                     WLNDGAHALRSLFSLAQQYRYSGTTRDDFAAKNAAARAALEKFGELPQDVLEGTRRSR
                     FAPPIARRTTKPGTPPAAPAIELDAPAHTDGTADGAAGDEAAGA"
     gene            1784..>1945
                     /locus_tag="VIO83_03345"
     CDS             1784..>1945
                     /locus_tag="VIO83_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3158 family protein"
                     /protein_id="HEY8330348.1"
                     /translation="MTASIPISHSTRFVALEQADFQRLEHAGYLKGLLQPFKGKGSLE
                     TWASQCAALR"
BASE COUNT          357 a          656 c          629 g          303 t
ORIGIN      
        1 agacgttcag cgcttgctgg gcagatgcct gttgcgcctt caacaatacg agcgcctcat
       61 gaaagccatc gtggcccacc acgagatttc aggcccggcg cattcgctgg aggccattcg
      121 cgcagcgcgg attgaagatg ccgcgaccaa gaccctgggc acgttgatcg gacaactgtt
      181 tggttcgtat gtcgtcaccg atggaaatgg cggcgaggaa cgcgacgacg atcttcccgg
      241 cgacgtgatc tcctttcgca cgcgcgtgca actgagcctg tctgcgcagg actacgccaa
      301 gacccaggcc gacctcaaag acctggtatc gctgcgcaac acactggtgc accacttcat
      361 cgaccagcac gatctatgga ccgtggacgg gtgccgcgct gcacaggacg aactcggttc
      421 cgcctacacg cgcatcgatc aacacttcga gcagttgcgc ggctgggccg agcacatgga
      481 tcaggcgcgg cgcctggcag cggaattcgt ccagtcggat gtgttccacg acctggtggt
      541 caatggcatc gcgccggacg gcacggtgga ctggccggcc gccggcatcg ttcgtgcgct
      601 acgcgaggcc gccgcgcagt tggccgtcga gggctggaca ccgatcgccg ccgctggccg
      661 ctggatcgcg gaccggcatc ccgagcagct tccagccaag tacggctgca gcagttggcg
      721 gcaggtggtg cacgagtgcc gcctgttcga gcttcgctac cgtgaggtgg aggggcaacg
      781 ggccgcctgg tacagacctc gcgaagcata gccccgcgac gcggtctcac ctccgcgcga
      841 gtcgcgaacg actccgccgg ctatccccag gggatagccg gcacacacag ccttccgcgc
      901 agaacaaacc gggcgcgcat gggctgcggt ttgttgactg acagccttcc ggccgatgcc
      961 gatactggcg actcgcccct tcaccgcgag tcgctctcat ggccaacgaa cgcatagaac
     1021 ccctgcaact gaatctcgga tcgctgcgca gcgcgatgtc gctgacgctg cacacgcacc
     1081 acgcttcgcg catctggcac ggccgcgcgc cgaccgaggg gcgcccaggc atcatcggtc
     1141 tcaacggctt catcggcgcc atgaacaaga tgcagcgcgg cgccgagcag gacgacccct
     1201 actcggactg gtggatgctg cggatcgagg acaagctcgc cgacaccaag acccgtctgc
     1261 agaccctgcg cgaacaggtg gatcaggcct tggccgacgt gccagcggcg ctgtccctgg
     1321 gcgagaacat gaacatgcag ccggttaagc tgccgctgtt cgtgaacgcc cagctcggct
     1381 tcatggcggt gtacctgctg gccgactacg acgacctggc acgcaaactc atcctggcgc
     1441 accacacggc gctgatcgac cgcagcacct tggagcgctg gctcaatgat ggtgcgcacg
     1501 cgctgcgcag cctgttctcg ctggcccagc agtaccgcta ctcgggcacc acgcgcgacg
     1561 acttcgcggc gaagaacgcg gcggcgcgag cggcgctgga gaagttcggc gagttgccgc
     1621 aggacgtgct ggaaggcacg cgccgctcgc gcttcgcgcc accgatcgcg cggcggacga
     1681 ccaagcccgg cacgccgcct gcagcgcccg ccatcgagct cgatgcgccg gctcacacgg
     1741 atggcacagc cgatggtgcg gcgggcgatg aggctgcggg cgcatgacag catcgatccc
     1801 cattagccac tccacgcgct tcgtggcgct ggaacaggcg gacttccagc ggctggaaca
     1861 cgcaggctac ctaaaaggcc ttttacagcc ttttaagggt aaggggagtc tggagacctg
     1921 ggccagccag tgcgcagcgc tgcgc
//
LOCUS       DATCUN010000083         2940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_266213, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000083 DATCUN010000000
VERSION     DATCUN010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266213"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /gene="ntrB"
                     /locus_tag="VIO83_03350"
     CDS             complement(<1..754)
                     /gene="ntrB"
                     /locus_tag="VIO83_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HEY8330349.1"
                     /translation="MGKSIRMKAALLSFVMLLVLLMVWQVASLPGKQAATSAMDEEYA
                     LLMGQTVSGETKEVNGMPTPAQFAGLAWKQLSDPFYDNGPNDKGIGIQLAHSLGRVGL
                     GYLIAALVAIPLGFVIGMSPLLYKAFDPFIQVLKPISPLAWMPIALYTIKDSTTSAIF
                     VIFICSVWPMLINTAFGVAGVRKDWLNVARTLEVGPLRKAFQVILPAAAPTIITGMRI
                     SMGIAWLVIVAAEMLIGGTGIGYFVWNEWNNLS"
     gene            complement(765..2159)
                     /locus_tag="VIO83_03355"
     CDS             complement(765..2159)
                     /locus_tag="VIO83_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8330350.1"
                     /translation="MTDKNFYRPYSEHSSLSGCTCGAHGSQQEHDLQAERERLARARD
                     SEALSRDFVEAAAVRALFPHDATRRRFLKAVGMGTAMAAIASVLPLQSLQAMAEEKGP
                     LEKKNLKIGFIPINCATPLIMADPMGFYKEQGLSVSLQKTAGWALVRDNMLNGELDAS
                     HFLAPMPLAISMGLGSSAQPMRVATIQNTNGQAITLALKHKDNRDPRNWKGFKFAIPF
                     EFSMHNFLLRYYLAEHGLDPDRDVQLRVTPPAEMIANLRAGNIDGFLGPDPFNQRAVY
                     DGIGFIHILSKEIWDGHPCCSFGTSQAFIEQNPNTFAALYRAILNAATAANDPANLTA
                     ISQAISTPNYLNQPEIVIRQALTGRYADGLGKVQNEPDRAGFDAMPWYSMATWMLTQM
                     KRWGYVKGEMNFNALAEQVFLMTDARRQMASLAMSEANAEPASGYKSFSVMGRPFDPR
                     QPQAYLDGFAIKRS"
     gene            complement(2187..>2940)
                     /locus_tag="VIO83_03360"
     CDS             complement(2187..>2940)
                     /locus_tag="VIO83_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005770374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8330351.1"
                     /translation="LCWRVNDLAGWSGLQAQLAKAPQRVAIVGAGMVGCELAEDFARA
                     GHQVTLLDRLALPLAGLLPEIAAQRLRQSQERLGVDYLGAVQVAGISAQDDGSKRITT
                     QCGQSLVVDQVVAATGLVTDDRLARIAGLDFDKGIRVDPCTLQTSAADVYALGDCVSL
                     DGAACRFIEPIARQARAIAHAVLGKAGESYAHSQPVIRLKTRSLPIEMHGTPCAEGEW
                     RVVREDEGCLLMEQQVDGRTASTLRVGQAKAA"
BASE COUNT          497 a          933 c          993 g          517 t
ORIGIN      
        1 gggacaggtt gttccactcg ttccagacga agtagccgat gccggtaccg ccgatcagca
       61 tttccgcagc gacgatgacc agccaggcga tgcccatgga aatgcgcatg ccggtgatga
      121 tggtcggcgc ggcggccggc aggatcacct ggaaggcctt gcgcagcggg ccgacctcga
      181 gggtgcgggc gacgttgagc cagtccttgc gcaccccggc gacaccgaag gcggtgttga
      241 tcagcatcgg ccagaccgag cagatgaaga tcacgaagat cgccgaggtg gtggagtcct
      301 tgatggtgta gagcgcgatc ggcatccagg ccagcggcga gatcggcttg agcacctgga
      361 tgaacggatc gaaggccttg tagagcagcg gcgacatgcc gatcacgaag cccagcggga
      421 tcgccaccag cgcggcgatc aggtagccga gccccacgcg ccccagtgaa tgggccagct
      481 ggatgccgat gcccttgtcg ttgggaccgt tgtcgtagaa gggatcgctg agctgcttcc
      541 aggccaggcc ggcgaactgc gccggggtcg gcatgccgtt gacctccttc gtttcgccgc
      601 tgacggtctg gcccatcagc agggcgtact cctcgtccat cgcgctggtg gcggcttgct
      661 tgccaggcag gctcgccacc tgccagacca tcagcagcac cagcagcatc acgaaggaca
      721 gcaacgcggc tttcatgcgt atcgatttgc ccatgacgtt ctccttagct gcgcttgatg
      781 gcaaagccat ccagataggc ctgcggctgg cgcggatcga acggccggcc catcaccgag
      841 aagctcttgt agccggacgc cggttcggca ttggcctcgc tcatcgccag gctagccatc
      901 tgccggcggg cgtcggtcat caggaacacc tgctcggcca gcgcgttgaa attcatctca
      961 cccttcacgt aaccccagcg cttcatctgg gtcagcatcc aggtcgccat ggagtaccag
     1021 ggcatcgcat caaagccggc gcgatccggc tcgttctgca ccttgcccag accgtcggca
     1081 tagcgcccag tcagcgcctg gcgaatgacg atctccggct ggttgaggta gttcggcgtg
     1141 gagatggcct gggaaatcgc cgtcaggttg gccggatcat tggcggccgt cgcggcattg
     1201 aggatggcgc ggtacagcgc ggcgaaggtg ttcgggttct gctcgatgaa cgcctgcgag
     1261 gtgccgaagg agcagcacgg atggccatcc cagatctcct tggaaaggat gtggatgaaa
     1321 ccgatgccgt cgtataccgc gcgctggttg aacggatccg ggccgaggaa gccgtcgatg
     1381 ttgccggcgc gcaggttggc gatcatttcc gccggcgggg tcacccgcag ctgcacatcg
     1441 cgatccgggt ccaggccatg ctcggccagg tagtagcgca gcaggaagtt gtgcatggaa
     1501 aactcgaagg ggatggcgaa cttgaagccc ttccagttgc gcggatcgcg gttgtccttg
     1561 tgcttgaggg ccagggtgat ggcctggccg ttggtgttct ggatggtcgc cacgcgcatt
     1621 ggctgggcgc tggaacccag gcccatggaa atggccagcg gcatcggggc gaggaagtgc
     1681 gaggcatcca gttcgccatt gagcatgttg tcgcgtacca gcgcccagcc ggcggttttc
     1741 tgcagactga ccgacaggcc ctgctcttta tagaagccca tcgggtcggc catgatcagc
     1801 ggcgtggcgc agttgatcgg gatgaagccg atcttcaggt tcttcttctc cagcgggccc
     1861 ttttcctcgg ccatcgcctg cagcgattgc agcggcagca cgctggcgat ggccgccatc
     1921 gcggtgccca tgcccaccgc cttgaggaag cggcggcgtg tggcatcatg ggggaacaac
     1981 gcacgcacgg cggccgcctc gacgaaatcg cgggacaggg cttcactgtc gcgggcgcgt
     2041 gccagacgct cccgctcggc ctgcaggtca tgctcctgct ggctgccgtg ggccccgcag
     2101 gtgcagcccg acaggctgct gtgctccgaa taggggcggt agaaattctt atcggtcatg
     2161 gctgtgccct cttttgggtc atccgatcag gctgctttgg cctgacctac acgcaaggtt
     2221 gaagcggtac gcccgtcgac ctgctgctcc atgagcaggc agccctcgtc ttcacgtacc
     2281 acacgccact ccccttcagc acaaggcgtg ccatgcatct cgatgggcag ggagcgggtc
     2341 ttcaggcgga tcaccggctg gctgtgggcg tagctctcgc cagccttgcc gagcacggca
     2401 tgggcaatcg ccctcgcctg ccgggcgatc ggttcgatga agcggcaggc agcgccgtcc
     2461 aggctgacgc agtcgcccag cgcgtagaca tccgcggcac tggtctgcag ggtgcaggga
     2521 tcgacccgga tgcccttgtc gaagtccagc ccggcaatcc gcgccaggcg gtcatcggtc
     2581 accaggccgg tggcggcgac tacctgatcg acgaccaggc tctggccgca ctgggtggtg
     2641 atgcgcttgc tgccatcgtc ctgggcgctg atgccggcga cctgcacggc accgagatag
     2701 tcgacgccga ggcgctcctg gctctggcgc aggcgctgtg ccgcaatctc cggcaacagg
     2761 ccggccagcg gcagggcaag gcggtcgagc agggtcactt gatggccggc gcgggcgaag
     2821 tcctcggcca gttcgcaacc gaccatgccc gcgccgacga tggccacccg ctgcggcgcc
     2881 ttggccagct gcgcctgcag gccgctccag ccggccaggt cgttgacccg ccagcacagc
//
LOCUS       DATCUN010000084         6288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_172620, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000084 DATCUN010000000
VERSION     DATCUN010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172620"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VIO83_03365"
     CDS             complement(<1..830)
                     /locus_tag="VIO83_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HEY8330352.1"
                     /translation="MKTLDQLEFDNRFARLGDTFSTRVQPQPIEQPRLVVASVAAMAL
                     LDLAPAEAQRPEFAELCSGHKLWQDAEPLAMVYSGHQFGQYNPQLGDGRGLLLGEVVN
                     AAGEHWDLHLKGAGYTPYSRMGDGRAVLRSSIREFLASEALAALGIPTTRALCVTAST
                     TPVYRERTETAAMLLRLSPSHLRFGHLEFFYYTRQHDALRQLAEHALDCHFPECREQE
                     QPFAAMFRTVVERTAATIALWQAYGFCHGVMNTDNMSLLGLTFDYGPYAFLDDFDARH
                     V"
     gene            complement(886..4212)
                     /gene="mscK"
                     /locus_tag="VIO83_03370"
     CDS             complement(886..4212)
                     /gene="mscK"
                     /locus_tag="VIO83_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HEY8330353.1"
                     /translation="MMFKLRPILASLLLSLLILAPFAGAANALPSAATVQKQLDALEA
                     RKLPEAEQKALQETLQQTLDHLQTVKSSTAELASLKQQLAVAPKQTLEAQRELAQLKP
                     VSTDELGKRYASLSTAQLESMLGERLAQLNDWQAQLAQATNALIDAQTRPERAQAEIS
                     ANQTRTQAIRATLKSGKDNNLPLGPEQLSMLQSELAALGAVSQLRSQELAGNTALQDL
                     SSNRRTLLTQRIALLEAEILALQNTLNTKRREESAETVAQLSRESLDNHRGSLLAAES
                     DINLELSNYLLKSTDRANELIRKNIETKQQLDSLKQAEQALDEQIAVLKGSLLLTRIL
                     YQQKNALPKVSADKDLADEIADLRLYQFELNQRRAEIINPIQHVDALLASQPGENATP
                     QLRSELEQLSRARLGLLDRLNTELGNLLTQAITLQLNQKQLLATSTSLSRTLDEQMFW
                     IASNKPLDLSWFKEVPSRLQNQLDALPVMDSARAIGHGLIGQPLLFLPLVVLLAALLT
                     GRPWLRRQLERIDAEIDQLRRGNQLLSPLAVLCHFLLVLPLPLIVFACGVVLIQDSQA
                     STTIFGESLLKLAQAMLVFGVTYRLLARGSVGERHFGWEPQRVQKLYRLVRLLGLVVM
                     PMVLVVSITQRQLSSLSEDVLGIFIVLAGFLLMSSILLRIIVNQPPFFGSRVLHFIAG
                     LLLVGLPLALVVAVGFGYYYTALKLTGRLLDSLYIIAIWSVLQAAILRGLDVAARRLA
                     LQRAQARRQQPVREGADGGELVEEPTLALEQVNQQSLRLVRLGLLLGFVGIFYFVWAD
                     LLAVFTYLDSVTLYEYTSGSGDTASKVPISLSDLLGALVILVITLILARNLPGLLEML
                     VLSRLNLAQGSAYATTTLLSYAIMAFGLVSTLSTLGLSWDKLQWLVAALSVGIGFGMQ
                     EIFANFISGLIILFERPVRIGDVVTIGNLSGSVSKIRIRATTIVDFDRKEIIVPNKTF
                     ITSQLVNWSLSDTVTRVTIKIGVAYGSDLDLVRRLLLEIARNNPRVLKEPEPTVFFLT
                     FNESTLDHELRIHVRELGDRNAAVDEINREIDRLFREHAIDIAFRQLDVLVKNLQGQH
                     LQLVSEQELPPSEASKTAKA"
     gene            complement(4334..6076)
                     /locus_tag="VIO83_03375"
     CDS             complement(4334..6076)
                     /locus_tag="VIO83_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEY8330354.1"
                     /translation="MEPAVVNSLFLVGALLVGISILVSAFSARLGIPVLVIFLAVGML
                     AGVDGIGGIVFNDYSTAFLVGNLALAVILLDGGMRTRVATFRVALWPALSLATVGVMI
                     TAGLTGLAAAWLFKLDLLEGLLIGAIVGSTDAAVVFNLLNGKGLNERVGSTLEIESGS
                     NDPMAMFLTVTLIDMLARGETGFSLHILLDLIQQFGIGIVLGLGGGWLLLQLVNRLAV
                     ADGLYPLLAVSGGIMIFAFAGEIGGSGILAVYTCGLMLGNRPIRNRHGILHMFDGLAW
                     LSQIGMFLVLGLLLTPHELLPIAVPALLLSLWMILCARPLSVFVGLLPFRAFHFRERL
                     FISWIGLRGAVPVILAVFPLVAGLPHAQLFFNVAFFIVLVSLLLQGTTLGWAARKTRV
                     EVPPQPAPISRAGLEVHPTSQWELFVYRLGAGKWCIGAHLRELKMPEGTRIAALFRGR
                     ELLHPSGSSRLEAGDVLCVIGHEKDLPALGKLFSQAPKRGQDLRFFGDFVLEGDAELG
                     PVAALYGLDLQGVDGSQQLGHFIAQQIGGEPVVGDQVEWAGRTWTVALMEGKHVRKVG
                     LKLPAGSAGPLLLL"
     gene            6095..>6288
                     /locus_tag="VIO83_03380"
     CDS             6095..>6288
                     /locus_tag="VIO83_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330355.1"
                     /translation="MTAKEKHAYQVPMDSNPLIWLVVEKYPGISRDAAALRAAGRIDG
                     RAAGGGLAAAKLLDPGGSALP"
BASE COUNT         1026 a         1932 c         2309 g         1021 t
ORIGIN      
        1 acgtggcggg cgtcgaagtc gtcgaggaag gcgtaggggc cgtagtcgaa ggtcaggccg
       61 agcagcgaca tgttgtcggt gttcatcacc ccgtggcaga agccgtaggc ctgccacagg
      121 gcgatggtgg cggcggtgcg ctcgactacc gtgcggaaca tcgcggcgaa cggttgctcc
      181 tgctcgcggc actcggggaa gtggcagtcc agcgcgtgct cggcaagctg gcgcagggcg
      241 tcgtgctggc gggtgtagta gaagaattct aagtggccga agcgcaggtg gctgggcgac
      301 aggcgcagca gcatggccgc ggtttcggtg cgctcgcggt acaccggggt ggtcgaggcg
      361 gtgacgcaca gcgcgcgggt ggtggggatg cccagcgcgg ccagcgcctc gctggcgagg
      421 aactcgcgga tcgatgagcg cagcaccgcg cggccgtcgc ccatgcgcga gtagggtgta
      481 tagccggcgc ccttgaggtg caggtcccag tgctcgccgg cggcgttgac cacctcgccg
      541 agcagcaggc cgcgaccgtc gccgagctgc gggttgtact ggccgaactg gtgcccggag
      601 tagaccatcg ccagcggctc ggcgtcctgc cacagcttgt gcccggagca cagttcggcg
      661 aactcggggc gctgcgcttc ggccggggcg aggtcgagca gggccatggc cgccacgctg
      721 gccaccacca gccgcggctg ctcgatgggc tgcggctgca cgcgggtgga gaaggtgtca
      781 cccaggcggg cgaagcggtt gtcgaattcg agctggtcga gggttttcac gaaggcctcc
      841 ggcaattccc gagcattgtg ctgggaattg ccgcgggccg tccagtcagg ccttggccgt
      901 cttgctcgcc tcgctcggcg gcagctcctg ctcgctgacc agttgcaggt gctggccctg
      961 caggttcttc acgagcacgt cgagctggcg aaaggcgatg tcgatggcgt gctcgcgaaa
     1021 caggcggtcg atctcgcggt tgatctcgtc caccgccgcg ttgcggtcgc ccagttcgcg
     1081 cacgtggatg cgcagttcgt ggtcgagcgt gctctcgttg aaggtgagga agaacaccgt
     1141 cggctccggc tccttgagca cccgcgggtt gttgcgggcg atctccagca gcagcctgcg
     1201 caccaggtcg aggtccgagc cgtaggccac gccgatcttg atggtgaccc gggtgacggt
     1261 gtcgctcagc gaccagttga ccagctggct ggtgatgaag gtcttgttgg ggacgatgat
     1321 ttccttgcga tcgaaatcga cgatggtggt cgcgcggatg cggatcttgc tcaccgagcc
     1381 ggacaggttg ccgatggtca ccacgtcgcc gatgcgcacc ggccgctcga acaggatgat
     1441 caggccggag atgaagttgg cgaaaatctc ctgcatgccg aaaccgatgc ccaccgacag
     1501 cgccgccacc agccactgca gcttgtccca gctgaggccg agggtggaca gggtgctgac
     1561 caggccgaag gccatgatcg cgtaggacag cagggtggtg gtggcgtagg cgctgccctg
     1621 ggccaggttg aggcgcgaca gcaccagcat ttccagcagg cccggcaggt tgcgcgcgag
     1681 gatcagggtg atgaccagga tcaccagggc gccgagcagg tcgctcaggc tgatcggcac
     1741 cttgctggcg gtgtcgccgc tgccgctggt gtattcgtag agggtcacgc tgtcgaggta
     1801 ggtgaacacc gccagcaggt cggcccagac gaagtagaag atgccgacga agccgagcag
     1861 caagcccagg cgtaccaggc gcagcgattg ctggttgacc tgctcgaggg ccagggtcgg
     1921 ttcctcgacc agttcgccgc cgtcggcgcc ctcgcgtacc ggctgctgcc ggcgtgcctg
     1981 ggcgcgctgc agggccaggc ggcgggcggc gacgtcgagg ccgcgcagta tcgccgcctg
     2041 cagcaccgac cagatggcga tgatgtacag gctgtcgagc aggcgaccgg tgagcttgag
     2101 cgcggtgtag tagtagccga agccgactgc taccaccagc gccagcggca ggccgaccag
     2161 cagcaggccg gcgatgaagt gcagtacccg cgagccgaag aacggcggct ggttgacgat
     2221 gatgcgcagc aggatgctgc tcatcagcag gaagccggcg aggacgatga agatccccag
     2281 cacgtcctcg gacaggctgc tgagctggcg ctgggtgatg ctcaccacta gcaccatcgg
     2341 catcaccacc agacccagca ggcggaccag gcggtagagc ttctgcaccc gctgcggctc
     2401 ccagccgaaa tgccgctcgc ccacgctgcc gcgggccagc aggcggtagg tgacgccgaa
     2461 caccagcatg gcctgggcca gcttgagcag cgattcgccg aagatggtgg tgctcgcttg
     2521 cgagtcctgg atcagtacca cgccgcaggc gaagacgatc agcggcagcg gcaggaccag
     2581 caggaagtgg cagagcaccg ccagcgggct cagcaactgg ttgccgcggc gcagttggtc
     2641 gatctcggcg tcgatgcgct cgagctggcg gcgcagccag gggcggccgg tgagcagggc
     2701 ggcgagcagc accaccagcg gcaggaacag cagcggctgg ccgatcaggc cgtggccgat
     2761 ggccctggcg ctgtccatca ccggcagagc gtcgagctgg ttttgcaggc ggctggggac
     2821 ctccttgaac cacgacaggt cgagcggctt gttgctggcg atccagaaca tctgctcgtc
     2881 gagggtacgg ctcaggctgg tgctggtggc gagcagctgc ttctggttga gctgcagggt
     2941 gatggcctgg gtcagcaggt tgcccagttc ggtgttgagg cggtcgagca gccccaggcg
     3001 ggcgcggctc agttgctcca gctcgctgcg cagctgcggg gtggcgttct cgccgggctg
     3061 gctggcgagc agggcgtcga cgtgctggat ggggttgatg atctcggcgc ggcgttggtt
     3121 cagctcgaac tggtaaaggc gcaggtcggc gatctcgtcg gccaggtcct tgtccgcact
     3181 gaccttgggc agggcgttct tctgctgata gagaatgcgg gtcagcagca ggctgccctt
     3241 gagcacggcg atctgttcgt cgagggcctg ttcggcctgc ttcagcgagt cgagctgctg
     3301 cttggtctcg atgttcttgc ggatcagctc gttggcgcgg tcggtggact tgagcagata
     3361 gttgctcagc tccaggttga tgtcggactc ggcggcgagc aggctgccgc ggtggttgtc
     3421 gagactttcg cgcgacagct gggcgacggt ttccgccgac tcctcgcgcc gcttggtgtt
     3481 gagggtgttc tgcagggcga ggatttccgc ctcgagcagg gcaatgcgct gggtcagcag
     3541 cgtgcggcgg ttgctgctca ggtcctgcag tgccgtgttg ccggccagct cctggctgcg
     3601 cagctgggat accgcgccga gggcggccag ctcgctctgc agcatgctca gctgttcggg
     3661 accgagcggc aggttgttgt ccttgccgct cttgagggtc gcgcgaatgg cctgggtgcg
     3721 ggtctggttg gcgctgatct cggcctgggc gcgctcgggc cgcgtttgcg cgtcaatcag
     3781 cgcattggtc gcctgggcca gctgcgcctg ccagtcgttg agctgggcca gtctctcgcc
     3841 gagcatgctt tccagctggg ccgtggacag gctggcgtag cgcttgccca gctcgtcggt
     3901 gctcaccggc ttgagctgcg ccagttcgcg ctgggcctcg agggtctgct tgggcgccac
     3961 ggccagctgc tgcttgaggc tggccagctc ggcggtgctg ctcttgacgg tctgcaggtg
     4021 gtcgagggtc tgctgcaggg tttcctgcag ggccttctgc tcggcctccg gcagcttgcg
     4081 cgcctcgagg gcgtcgagct gtttctgcac ggtagcggca ctgggcaggg cgttggcggc
     4141 gccggcgaag ggcgcgagga tcagtagact cagcagcagc gaagccagga tcgggcgtag
     4201 cttgaacatc atggcgaaca cggttttttg ttgaggaacc ggcaagccta ctggggctgt
     4261 cctgttgcga caaggctatc tgtccggaag tggcgctggc gatgggcgcg gcgagcaccg
     4321 ccagacgctc gattcagagc agcaacagcg gccccgcgct gccagccggc agcttcaggc
     4381 cgaccttgcg gacgtgcttg ccttccatca gcgccaccgt ccaggtacgc ccggcccact
     4441 cgacctggtc gccgaccacc ggctcgccgc cgatctgctg ggcgatgaag tggccgagct
     4501 gctggctgcc gtcgacgccc tgcaggtcga ggccgtagag cgcggccacc ggccccagct
     4561 cggcatcgcc ctcgagcacg aaatcgccga agaagcgcag gtcctggccg cgcttgggcg
     4621 cctggctgaa cagtttgccg agggccggca ggtccttctc gtggccgatc acgcagagca
     4681 cgtcgcccgc ttcgagacgg ctgctgccgc tggggtggag cagttcgcgg ccgcgaaaca
     4741 gcgccgcgat gcgcgtgccc tcgggcatct tcagctcgcg caggtgcgcg ccgatgcacc
     4801 atttgccggc acccagccgg tagacgaaca gctcccactg gctggtgggg tggacttcca
     4861 gcccggcgcg cgagatcggc gccggttgtg gcgggacctc gacgcgggtc ttgcgcgccg
     4921 cccagcccag ggtggtgccc tgcagcagca gggagaccag cacgatgaag aacgctacgt
     4981 tgaagaacag ctgggcgtgg ggcaggccgg cgaccagcgg gaacactgcg aggatcaccg
     5041 gcaccgcgcc gcgcagaccg atccaggaaa tgaacaggcg ctcgcggaag tgaaaggcgc
     5101 ggaacggcag caggccgaca aataccgaca gcgggcgggc gcagaggatc atccacagcg
     5161 acagcagcag cgctggcacc gcgatcggca gcagctcgtg cggcgtcagc agcaggccga
     5221 ggaccaggaa catgccgatc tggctgagcc aggccaggcc gtcgaacatg tgcaggatgc
     5281 cgtggcggtt gcggatcggc cggttgccca gcatcaggcc gcaggtgtac acggccagga
     5341 tgccgctgcc accgatttcc ccggcgaagg cgaagatcat gatgccgccg ctgaccgcca
     5401 gcagcggata caggccgtcg gccaccgcca ggcggttgac cagctgcagc agcagccagc
     5461 cgccgcccag gccgaggacg atgccgatgc cgaactgctg gatcaggtcg agcaggatat
     5521 gcaggctgaa gccggtctcg ccgcgggcga gcatgtcgat cagggtcacg gtgaggaaca
     5581 tcgccatcgg gtcgttgctg cccgattcga tttccagggt cgagccgacc cgttcgttga
     5641 gacccttgcc gttgagcagg ttgaacacca cggcggcgtc ggtggagccg acgatggcac
     5701 cgatcagcag gccttccagc aggtcgagct tgaacagcca ggcggcggcg aggccggtca
     5761 gccccgcggt gatcatcacc ccgacggtgg ccagcgacag cgccggccac agggcgacgc
     5821 ggaaggtcgc gacccgggtg cgcatgccgc cgtcgaggag gatcaccgcc agcgccaggt
     5881 tgccgaccag gaaggccgtg gagtagtcgt tgaacacgat gccgccgatg ccgtcgaccc
     5941 cggcgagcat gcccacggcg aggaagatca ccagcaccgg aatgcccagg cgcgcggaga
     6001 aggcgctgac caggatgctg atgcccacca gcagagcgcc gacgaggaac aggctattga
     6061 cgacggctgg ctccaagggg atctccataa gtggttgacg gcaaaagaaa agcatgcata
     6121 tcaagtgcca atggattcta acccgttgat ttggcttgtg gttgaaaaat atccgggaat
     6181 ttccagggac gccgcagcgc tgcgggcggc ggggcgcatc gatggcaggg ctgccggtgg
     6241 cggcctcgcg gctgctaaac tgctcgaccc tggcgggagc gcattgcc
//
LOCUS       DATCUN010000085         2811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_22185, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000085 DATCUN010000000
VERSION     DATCUN010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22185"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..107
                     /locus_tag="VIO83_03385"
     CDS             <1..107
                     /locus_tag="VIO83_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEY8330356.1"
                     /translation="YGTCAACGASIAPARLLAVPTAELCLDCAGHARD"
     gene            306..2783
                     /locus_tag="VIO83_03390"
     CDS             306..2783
                     /locus_tag="VIO83_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8330357.1"
                     /translation="MPDFLPSLRLLPSQLSSQFSAEQLTFTSTDELEPFRGVLGQERA
                     VAALQFGVAMPRPGYNVFVMGESGTGRFSFVRRYLKAEAKRQATPCDWVYVNNFDDPR
                     EPRALQLPSGRAQALMDDIDQLIDNLLATFPAAFETPSYQQKKSAIERVFNQRYDRAL
                     DVIEKLALEKSIALYRDSANIAFTPMKDGQALDEAEFGQLPEADRERFHDDIAGLEER
                     LNEELASLPQWKRESSNQQRQLNEETITVALQPLLAPLSQKYAENAGVCGWLQAMQVN
                     LLKTVVDQLVEDTRPDAQKRQSLVEFYSPSLVVGHAHGGGAPVVFEPHPTYDNLFGRI
                     EYSSEQGALYTSYRQLRSGALHRANGGFLILEAEKLLSEPFVWDALKRALHSRQLKME
                     SPLAELGRLATVTLTPQVIPLQVKVVIVGARQIYYALQELDPDFQEMFRVLADFDEDM
                     PLAEDSLEQFAQLLKTRTSEEGMAPLSNDAVARLATYSARLAEHQGRLSARIGDLFQL
                     VSEADFMRQLAGETVTDVGHIERALRAKATRTGRVSARILDDMLAGVILIDTAGAAMG
                     KCNGLTVLEVGDSAFGVPARISATVYPGNSGIVDIEREVSLGQPIHSKGVMILTGYLG
                     SRYAQEFPLEISASIALEQSYGYVDGDSASLGEVCTLISALSRTPLKQCFAITGSINQ
                     FGEVQAVGGVNEKIEGFFRLCEGRGLSGEQGVIIPHANVCNLMLDERVIQAVQAGLFS
                     VYAVRTVDEALSLLVGELAGTADDRGVFPEGSVNARVVERLREIAEMGDDEEEDEDEE
                     GAEPHGKAAAAAPVPGKPTSQALRLRR"
BASE COUNT          448 a          969 c          971 g          423 t
ORIGIN      
        1 gctatggcac ctgtgccgcc tgcggggctt ccatcgcgcc cgcgcggctg ctggccgtgc
       61 cgaccgccga actgtgcctg gactgtgccg gccacgccag ggactgaacc gacgcgctcg
      121 gccggcgcct gtgctgctct gcctggggag gagggccgcg ctgccgatgg ctgccgccgc
      181 gggtactgta cgacaggcgc tccggctgct tcttgtcaat cccgggcgcc gggtcaacaa
      241 tcggcagcat tactgctccg cagccgcgct gccgcgcatg cccgtcactc ttgtggaccc
      301 cgttgatgcc cgatttcctc ccctcgcttc gcctgctgcc ttcgcagctc agcagccagt
      361 tcagtgccga acagctgact ttcaccagta ccgacgagct ggagccgttc cgcggtgtgc
      421 tcggccagga gcgcgcggtg gccgccctgc agttcggcgt ggccatgccg cggcccggct
      481 acaacgtgtt cgtcatgggc gagtcgggca ccgggcgctt ctccttcgtg cgccgctacc
      541 tcaaggccga agccaagcgc caggccacgc cgtgcgactg ggtctacgtc aacaacttcg
      601 acgatccgcg cgaaccgcgt gcgctgcagc tgccctcggg gcgcgcgcag gcgctgatgg
      661 acgacatcga ccagctgatc gacaacctgc tggcgacctt tccggcggcg ttcgagacgc
      721 cgagttacca gcagaagaag agcgccatcg agcgcgtctt caaccagcgc tacgaccgcg
      781 ccctggacgt gatcgagaag ctggcgctgg agaagagcat cgccctgtac cgcgacagcg
      841 ccaacatcgc cttcacgccg atgaaggacg gccaggcgct ggacgaggcc gagttcggcc
      901 agctgccgga ggccgaccgc gagcgcttcc acgacgacat cgccggactc gaggagcgcc
      961 tcaacgagga gctggccagc ctgccgcagt ggaagcgcga gtcgagcaac cagcagcgcc
     1021 agctcaacga ggaaaccatc accgtggcgc tgcagccgct gctggcgccg ctgtcgcaga
     1081 agtacgcgga aaacgccgga gtctgtggct ggctgcaggc catgcaggtc aatctgctga
     1141 agaccgtggt cgaccagctg gtcgaggaca cccgcccgga tgcgcagaag cgccagagcc
     1201 tggtggagtt ctacagcccc agcctggtgg tcggccatgc ccatggtggc ggtgcgccgg
     1261 tggtattcga gccgcatccg acctacgaca acctgttcgg ccgcatcgag tacagctccg
     1321 agcagggcgc gctgtacacc agctaccgcc agttgcgctc gggcgcgctg caccgggcca
     1381 acggcggctt cctgatcctc gaggcggaaa agctgctgtc cgagccgttc gtctgggacg
     1441 cgctcaagcg cgccctgcat tcgcgccagc tgaagatgga gtcgccgctg gccgaactcg
     1501 gccggctggc caccgtcacc ctgacccctc aggtgatccc gctgcaggtc aaggtggtga
     1561 tcgtcggcgc gcggcagatc tactacgcgc tgcaggagct ggatccggat ttccaggaga
     1621 tgttccgcgt gctggccgac ttcgacgagg acatgccact ggccgaggac agtctcgagc
     1681 agttcgccca gctgctgaag acgcgcacct ccgaggaggg catggcgccg ctgagcaacg
     1741 atgcggtggc ccgtctggcc acctacagcg cgcgcctggc cgagcaccag ggccgcctct
     1801 cggcgcgcat cggcgacctg ttccagctgg tcagcgaggc cgacttcatg cgccagctgg
     1861 ccggcgagac ggtgaccgac gtcggccaca tcgagcgcgc gctcagggcc aaggccaccc
     1921 gcaccggccg ggtgtcggcg cgcatcctcg acgacatgct ggccggggtg atcctcatcg
     1981 acaccgccgg tgcggcgatg ggcaagtgca acggcctgac cgtgctggaa gtcggcgact
     2041 cggcgttcgg cgtgccggcc cgcatctccg ccaccgtcta cccgggcaac tcggggatcg
     2101 tcgacatcga gcgcgaggtc agcctcggcc agccgatcca ctccaagggg gtgatgatcc
     2161 tcaccggcta ccttggtagc cgctacgccc aggagtttcc cctggagatc tcggcgagca
     2221 tcgccctcga gcagtcctac ggctacgtcg acggcgacag cgcctcgctc ggcgaggtgt
     2281 gcacgctgat ctcggcactg tcgcgcacgc cgctcaagca gtgcttcgcc atcaccggct
     2341 cgatcaacca gttcggcgag gtgcaggcgg tcggcggggt caacgagaag atcgagggct
     2401 tcttccgcct ctgcgagggg cgcggcctct ccggcgagca gggggtgatc atcccgcacg
     2461 ccaacgtctg caacctgatg ctcgacgagc gggtgatcca ggcggtgcag gccgggctgt
     2521 tcagcgtcta cgcggtgcgc acggtggacg aggcgctgag cctgctggtg ggcgagctgg
     2581 ccggcacggc cgacgaccgt ggcgtgttcc ccgagggcag cgtcaatgcg cgggtggtcg
     2641 agcgtctgcg cgagattgcc gaaatgggcg acgacgagga ggaggacgag gacgaggagg
     2701 gcgcggaacc gcatggcaag gccgcagccg ccgcgcctgt gccgggcaag ccgaccagcc
     2761 aggccctccg cctgcgccgc tgaggggctg cggcggattg ggcggcgacc g
//
LOCUS       DATCUN010000086         5891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_172851, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000086 DATCUN010000000
VERSION     DATCUN010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172851"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1337
                     /locus_tag="VIO83_03395"
     CDS             48..1337
                     /locus_tag="VIO83_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY8330358.1"
                     /translation="MFPSKTRKWLPCALLVLATGSSLAQTAPSASSLQSVTAGPLSLK
                     QAFDTAWARQPEAESSSARREAATARRAAADSWTAEPMALELSSKGDQLNGNDGSREH
                     NVGLAIPLWLPGERSRTGAVAEAEAQSTASRASAAQLRTAAEVREAYWQWQRSRVEAS
                     LAREREGNARQLAADVAKRVKSGELALADQHQAEGAAAQAEIEAARARSALTTAAQQL
                     RSLIGTLPGGREETAPGVVAGEPVPALPADFAVLDAQHPAVAALLDQAEVARRSAELV
                     RVQTRANPELTLSTSREREPGESYDESLTLGIRIPFGSGSRNRAKQATAEAEAIEAEV
                     QARLARERLLGELEAARLEFESARAQVTAAERRARLAQESRGFFEKSFRLGETDLPTR
                     LRIELEATDAKRQAALARLDQAAATSSLRQALGLLPE"
     gene            1367..1912
                     /locus_tag="VIO83_03400"
     CDS             1367..1912
                     /locus_tag="VIO83_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330359.1"
                     /translation="MTLFPRLTAFTVATLIAAAPLATQAGEGHDHGEAPATPTGQALP
                     RFAATSDSFELVGVLDGHDLTLWLDHAADNSPVEGASLELEIGGVKVEAEQHEAGEFE
                     AELAEEPEEGEIAIKAKVVVGQRTELLSGDLDIHHDEHGEESASGASWGTYGAWGAGG
                     LVLLALLGWAMRRARVAPRHS"
     gene            1922..3031
                     /locus_tag="VIO83_03405"
     CDS             1922..3031
                     /locus_tag="VIO83_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY8330360.1"
                     /translation="MKTNFLISAVCLALLSLTSPAWAGEGHDHGEEAPATNSNGPQRL
                     PDGSVFLPKPAQRQIGVRTLPLETAELPRSLELAGKVVMDPNAGGMVQAVLAGRLEAG
                     PRGFPSVGQAVKKGEVLAYVVATGDVLERANQAAQLAQLRAAKGLADQRLVRLRELAD
                     TVPRKDIEAAASEVASLAAQIAALSSGINAREALLAPVSGVIASSNAVAGQVVDAREL
                     VFEVVDPSRLRIEALAYDLSQAADVAGATLAVGSERVPLTFIGAARSLREQALPMLFQ
                     GEGSALSRLAVGQPVRVFVQTASRTQGIRVPAASLMKNAANQSVVWVKTAPEHFAPRP
                     VTFVALDGVSVAVTSGLAAGDRVVTQAATLINQIR"
     gene            3046..>5891
                     /locus_tag="VIO83_03410"
     CDS             3046..>5891
                     /locus_tag="VIO83_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8330361.1"
                     /translation="MFKWLLDNSLGNRLLVIIASLVLMAYGAFTLSRMPVDVFPDLNK
                     PTVTIMTEAGGMAAEEVEQLITFPLETTMNGLPGVESVRSVSSSGLSFIYVTFDWSTE
                     IFRARQMVSERLSAMEEGLPPGVIPRMGPISSIMGEIMQIAIPIDADKITPMQVREFA
                     DWVLRPRLMAIPGVAQVIPIGGEVRQFQVQPDTARMAELGISHTQLEEALKGYSSNTS
                     GGFLELNGREYLIRNLGRTSSLADLSNLAITARNGQPILLRQIAEVTFAAALKRGDAG
                     FEGKAAVILGIQKQPTADTIQLTRSIEAALGEMRKSLPTGMEAPQVTFRQASFIEASI
                     STLQGKLIGASVFVAAILFFFLGTLRPTIIALTAIPVSIFITGLVFKYFGLSINTMTL
                     GGLAIAIGGLVDDAVVGVENVLRRLKVERAKHPEHRLHPLELVGHATMEVRSAILYAT
                     IIIVLVFLPLFALPGMEGRLFVPLGIAFIVSTLASLVVSVTVTPVLSFYLLPQMKSLE
                     HGDTKLLAWLKSRYRSSLQAVLQRPKAAVVAGGIAVLVAAASVPFFPKTFLPPFNEGT
                     LLIGLRLNPGVTLAESSALAQQAEQLVMQVPEVTHVGRRSGRAELDEHAEGVHVSELD
                     VGLKPASELTRSMDEITGDIRSRLVNLPAAIGIGQPISHRIDHMLSGVRSQIAIKIFG
                     EDLDTLRGQADALRGRLAAIPGIADLEIEKQVLAPQIKVRIDYAAAAQYGVPAPQILA
                     TLESLVEGEKVTQIVEGGRRFALVVRLPETARSAEGLGNILFETPTGRVPLSRIASIE
                     DGDGPNQISRDDGKRRIVLSANAQGRPLSEVVEDIRKVVDESKLPEGYFITLGGQFQA
                     QEEASRLVGLLSLVSLVLMFVVLYSRYKSAILASLIMVNIPLALVGAVLGLWLSGQPL
                     SIAALIGFITLAGISVRNGILKVSHYINLMRIEG"
BASE COUNT          942 a         1955 c         1990 g         1004 t
ORIGIN      
        1 gcgtctcgcc gattccctgt gagctttttc atcactggag aatcgggatg ttcccaagca
       61 aaaccagaaa atggctgccc tgtgccttgc tcgtgctggc caccggcagt tccttggcgc
      121 agacagcgcc aagcgcttcc agcctgcagt cggtcactgc cggtccttta tcgctgaagc
      181 aagccttcga taccgcttgg gcgcgtcagc ccgaagcgga gtcctcgtct gcgcgtcgtg
      241 aggcggccac cgcccgtcgc gccgccgccg acagctggac cgccgagccc atggccctgg
      301 agctgtcgag caaaggcgac cagctcaatg gcaacgatgg tagccgcgag cacaatgtcg
      361 gcctcgccat ccccctgtgg ctgcccggcg agcgctcgcg tacgggcgcc gtggccgaag
      421 ccgaggccca gtccaccgcc agtcgcgcct cggctgcgca gctgcgtact gctgctgaag
      481 tgcgcgaggc gtactggcag tggcaacgca gccgggtcga agcgagcctg gcccgcgagc
      541 gcgagggcaa tgcccggcag ctcgctgccg atgtcgccaa gcgggtcaag tcgggcgagc
      601 tggccctggc cgatcagcac caggccgagg gtgctgcggc acaggccgag atcgaggcgg
      661 cgcgagccag gagtgcgctg acgacagctg cacaacaatt gcgcagcctg attggcaccc
      721 tgccgggtgg tcgcgaagag actgcgcctg gcgtagttgc cggtgagccg gtacccgcgc
      781 tgcccgccga cttcgctgtg ctggatgccc agcatccggc ggtggccgcc ctgctcgatc
      841 aggccgaggt cgcgcgccgc tcggccgagc tggttcgcgt gcagacccgc gccaatcccg
      901 agttgacctt gtctaccagc cgcgaacgcg agccggggga gtcctatgac gaaagcctca
      961 ccctgggcat tcgcatcccc ttcggctcgg gcagccgcaa ccgcgccaag caagccacgg
     1021 ccgaggccga ggccatcgaa gccgaggtgc aggcgcgtct ggcgcgtgag cgcctgctcg
     1081 gcgagctgga ggccgcccgg ctggagttcg aatccgctcg cgcccaagtg acggctgccg
     1141 agaggcgtgc ccggctcgcc caggagtctc gcggtttctt cgaaaaatcc ttccgcctcg
     1201 gcgaaaccga cctgccgacg cgcctgcgta tcgaactcga ggcgaccgac gccaagcgtc
     1261 aggctgccct ggcccgactc gatcaggcgg cggcgacctc ctcactgcgc caggcgctgg
     1321 gtctgctgcc cgaatagggc cagaacgaac ctaaaggact gcaaagatga cactgtttcc
     1381 aaggctgaca gccttcactg tggcgacctt gatcgctgcc gcccccttgg ccactcaagc
     1441 cggcgagggg catgaccatg gcgaagcgcc ggccactccg actggacagg ccctgccgcg
     1501 cttcgcggcg acttccgact ccttcgagtt ggtcggcgtg ctcgatggcc acgacctcac
     1561 tctgtggctc gaccatgcag ccgacaacag tccggtcgag ggcgcgagcc tcgaactgga
     1621 gattggcggc gtcaaggtcg aggccgagca gcacgaagcc ggcgagttcg aggccgagct
     1681 ggcggaagag cccgaggaag gcgagatcgc catcaaggcc aaggtcgtcg tcggccagcg
     1741 cacggagctg ctgagcggtg atctggatat ccatcacgac gagcacggcg aagagtctgc
     1801 atctggcgcg tcctggggca cctacggtgc ctggggtgcc ggcgggctgg ttctgctcgc
     1861 cctgctgggt tgggccatgc gccgcgcccg cgtcgctcct cgtcattcgt gaaggtgttc
     1921 catgaaaact aattttctga tttccgcggt gtgcctggct ctgctgtcgc tgacctcgcc
     1981 cgcctgggct ggcgaagggc atgaccatgg cgaagaagcg cctgcaacca acagcaacgg
     2041 cccccagcgc ctgcccgacg gcagcgtatt cctgcccaag ccggcgcaac gccagatcgg
     2101 ggtgcgcacc ttgccgctcg agacggccga attgccgcgc tcgctggagc tggccggcaa
     2161 ggtggtgatg gaccccaatg ccggcggcat ggtgcaggcc gtgctggctg ggcgcctcga
     2221 agccgggccg cgcggctttc ccagcgtcgg ccaggcggtg aaaaagggtg aggtgctggc
     2281 ctacgtcgtg gcaaccggcg atgtcctcga acgggccaat caggctgcgc aactggccca
     2341 gctgcgggcg gccaagggcc ttgccgacca gcgtctggtg cgcttgcgcg agctggccga
     2401 tacggtgccg cgcaaagaca tcgaggcggc ggccagcgag gtcgcgagct tggcggcgca
     2461 aatcgctgcg ttgagctccg gtatcaatgc tcgggaagca ctgcttgcgc cggtctccgg
     2521 ggtgattgcc tcgagcaacg cggtcgccgg ccaggtggtc gatgctcgcg aactggtgtt
     2581 cgaagtggtc gatccctccc gcctgcgcat cgaggcgctg gcctacgacc tgagccaggc
     2641 cgccgatgtg gcgggagcca ctctggcggt gggcagcgag cgtgtgccgc tgaccttcat
     2701 cggcgcggcg cgcagcctgc gcgaacaggc cctgccgatg ctgttccagg gcgagggaag
     2761 cgccttgtcc cggctggccg tggggcagcc ggtgagggtc ttcgtgcaga ccgccagtcg
     2821 tacccagggc atccgggttc cggcagcgtc gctgatgaag aatgccgcca accagagtgt
     2881 cgtctgggtg aagaccgcgc cggagcactt cgcgccgcgc ccggtcacct ttgtcgcgct
     2941 ggacggggta tccgtggcgg tgaccagcgg gcttgccgct ggggatcgcg tggtgaccca
     3001 ggccgccact ctgatcaacc agatccgcta aggggagcct gacccatgtt caagtggctc
     3061 ctcgataaca gtctcggcaa ccgcctgctg gtgatcatcg ccagcctggt gctgatggcc
     3121 tatggcgcct tcaccctgtc acgcatgccg gtggatgtct ttcccgatct gaacaagccc
     3181 acggtcacca tcatgaccga ggccggcggc atggccgccg aagaggtcga gcaactgatc
     3241 accttcccgc tggagaccac catgaacggt ctgcccgggg tggagagtgt gcgctcggtc
     3301 tccagcagcg ggctgtcctt catctacgtc accttcgact ggagcacgga gattttccgc
     3361 gcccggcaga tggtttcgga acgcctgtcg gcgatggagg agggcttgcc accgggcgtg
     3421 atcccgcgca tggggccgat cagctccatc atgggcgaga tcatgcagat cgcgattccc
     3481 atcgatgcgg acaagatcac gccgatgcag gttcgcgagt tcgccgactg ggtgctgcgc
     3541 ccgcgcctga tggcgatccc cggtgttgcc caggtgatcc cgattggcgg cgaggtccgc
     3601 cagttccagg tccagcccga cacggcgcgg atggccgagc tgggtatcag ccatacgcaa
     3661 ctggaggagg cgctcaaggg ctattcgtcg aatacctccg ggggcttcct cgagcttaat
     3721 ggccgcgagt acctgattcg caacctgggg cggacgtcga gtctggcgga tctgtcgaac
     3781 ctggccatca ctgcccgtaa cggccagccg atcctgctgc ggcagatcgc cgaagtgacc
     3841 tttgccgcag ccttgaagcg cggcgacgcc ggcttcgaag gcaaggccgc ggtgatcctc
     3901 ggcatccaga aacaaccgac ggccgatacc atccagctga cccgctccat cgaggcggcg
     3961 ctgggggaaa tgcgcaagtc gctgccgacc ggcatggagg ctccccaggt caccttccgc
     4021 caggccagct tcatcgaggc ttcgatcagc accctacagg gcaagctgat cggcgcctcg
     4081 gtgttcgtcg cggcgatcct gttcttcttc ctcggcacgc tgcgccccac catcattgcg
     4141 ctgaccgcca ttccggtgtc gatcttcatc accggcctgg tgttcaagta cttcggcctg
     4201 tcgatcaaca ctatgaccct cggaggcctg gccatcgcta tcggtggatt ggtcgacgat
     4261 gccgtggtgg gcgtggaaaa cgtgctgcgc cggctgaagg tggagcgggc caagcacccc
     4321 gagcaccgcc tgcacccact ggaactggtg ggacacgcga ccatggaggt gcgttcggcc
     4381 atcctctacg ccaccatcat catcgtgctg gtgttcctgc cgctgttcgc cctgccgggc
     4441 atggaagggc ggctgttcgt accgctgggg atcgccttca tcgtctcgac cctggcctcg
     4501 ctggtggtct cagtgaccgt caccccggtg ctgtccttct atctgttgcc gcagatgaag
     4561 tcgctggagc atggcgacac caagttgctg gcctggctca agagccgcta tcgcagcagc
     4621 ttgcaggccg tcctgcagcg tccgaaggcg gctgtagtcg caggtggcat cgccgtgttg
     4681 gtcgcggcgg cgagcgtgcc gttcttcccg aagaccttcc tgccgccgtt caacgagggc
     4741 accttgctga tcggcctgcg tctgaacccc ggtgtgaccc tggcggagtc ctcggcgctg
     4801 gcccagcagg ccgagcagtt ggtcatgcag gtgccggaag tgacccacgt cggccgccgc
     4861 agcggccgtg cggagctgga tgagcacgcc gagggtgtgc acgtcagcga gctggatgtc
     4921 ggcctcaagc cggcctcgga gctgacccgc tcgatggatg agatcaccgg cgacatccgc
     4981 tcgcggctgg tcaatctgcc ggcggccatc ggcatcggtc agccgatctc gcaccggatc
     5041 gaccacatgc tgtccggcgt gcgctcgcag atcgccatca agatcttcgg cgaggatctg
     5101 gacacactgc gcggccaggc agatgccctg cgcggcaggc tggcggcgat tcccggcatc
     5161 gccgacctgg agatcgagaa gcaggtgctg gctccgcaga tcaaggtgcg catcgactac
     5221 gccgccgccg cccagtacgg cgtgcccgcg ccgcagatcc tggcgacgct ggagagtctg
     5281 gtcgagggcg aaaaggtcac ccagatcgtc gagggtggcc ggcgtttcgc cctggtggtg
     5341 cgcctgcccg agacggcacg ttcggcggag ggcctgggca acatcctgtt cgagaccccg
     5401 accggccggg tgccgctgtc gcggatcgcc agcatcgagg acggcgacgg accgaaccag
     5461 atcagtcgcg acgacggcaa gcgccgcatc gtgttgtcgg ccaacgctca ggggaggccg
     5521 ctgtcggagg tcgttgagga tatccgcaag gtggtcgacg agagcaagct gccggaaggc
     5581 tacttcatca ccctcggcgg ccagttccag gcccaggagg aggcttcgcg gctggtcggt
     5641 ctgctgtccc tcgtctcgct ggtgctgatg ttcgtggtgc tctacagccg ctacaagtcg
     5701 gcaatcctgg cctcgctgat catggtcaac atcccgctcg ctctggtcgg cgcggtgctg
     5761 ggcctgtggc tgtcggggca gccgctgtcg atcgccgcgc tgatcggctt catcaccctg
     5821 gccggcatct cggtgcgcaa cggcatcctc aaggtcagcc actacatcaa cctgatgcgc
     5881 atcgagggcg a
//
LOCUS       DATCUN010000087         5590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_172977, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000087 DATCUN010000000
VERSION     DATCUN010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172977"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VIO83_03415"
     CDS             <1..1171
                     /locus_tag="VIO83_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HEY8330362.1"
                     /translation="CARCNQLRQQAIQLLAEALPAWSNRVRLQDARLRDSLFAFAPGI
                     DSPPRYASLLQDEGHARLLGLAPQGLVADLREYLLLGRGQRVHANLPVPYGITDELLQ
                     HLCTTWSAPAERAYNRTPANGSLSACLGISALHYFVAGHRSFAEVLQLPDEEVTVDFQ
                     RRQREQDTWARAFDAGHNEALLHKERERIDFTPVGKQAAAPAPEREDYPRHQLQIVNQ
                     SPGGYCLAWDDSPPSQLQSGDLVGLQHPGERGWNIALIRWIRQPHGAGLQLGIELLAP
                     RAHPCGLRPQPRSGQPGQYLRGLLLSAIPALGRPATLIAPHLPFQQGQKILLNLDGEV
                     QRILLTHRHALSDSFNQFEYRRLDARVDLERKPVTGLSIVSEEPAEGFDSLWESL"
     gene            1237..3306
                     /locus_tag="VIO83_03420"
     CDS             1237..3306
                     /locus_tag="VIO83_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8330363.1"
                     /translation="MSNEKKTIRLLILEDSQNEAERLISLFRNAGHATRVHRLTSGDD
                     LADALQQSWDLLIAAPHSENLDPGEALSAIRRQGKDIPFLQRVADSDSEAITEAMLLG
                     AQDGVPQGDDERLLLVAGRELKNLEERRARRAAEVALREAEKRCQLLLDSSVDAITYV
                     HEGMHIYANRAYVELFGYPDADDLAGMPMIDLIHSGDQAAFKDFLKTYQSSEGHADLQ
                     CSGVRDDGSQFRARMTFSPATYDGEPCTQVVIRAETGNAELEEKLREISSQDLVTGLF
                     NRVHFLELLDQACERAVKDGSAATLALLRIDRYAALLSEIGLADGDLLLADCANLLRQ
                     HFGDRAQLARLADDSFAALLPTLTPQQSEEALHGLLKKIEGHLFDIGGRTTQITLSIG
                     VAGISEQSAKSQQVIERVHRCVEQLSNGNALKLYDPAEELAAAASRGSLVAMVQQALD
                     NNSFQLLFQPIISLHGDERENYEVLLRLSNPQGEPVAPAEFLAAAQQAGLAEKVDRWV
                     LLNSIRQLGEQRAKGGNTRLFIPLGAASLQDPVLLPWLSAALKAARLPADALVLQFCE
                     PDAVAYLKQAKTLSQGLAELHCKVALSQFGGALNPFNTLRHLHVDFVKIDGSLIDNLA
                     EAERQEQLKSLIGGLHAQAKLTIVPGVDNPSVLTTLWQTGVNYVQGLYLQGPSPRMDY
                     EFSSGEE"
     gene            complement(3369..4583)
                     /gene="serB"
                     /locus_tag="VIO83_03425"
     CDS             complement(3369..4583)
                     /gene="serB"
                     /locus_tag="VIO83_03425"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEY8330364.1"
                     /translation="MREIVLINITGEDRPGLTAAITGVLARGGVNILDIGQAVIHNTL
                     SFGILVEIPHSAKASSVLKDLLFTGYELDQQVRFTPVSEADYQQWVGGQGKPRHIVTL
                     LTRKVTAEQLQRVSSITAQYGLNIDHIDRLSGRMPLDTPDERGKGCIEFSVRGEPADP
                     AALRAEFLSVAQELNVDIAFQQDTIFRRNRRLAVFDMDSTLIEAEVIDELAKAAGVGE
                     QVAEITERAMRGELDFRASFKERLALLKGLSEEVLADIGAGLRMTEGAEFLFAELKRL
                     GYKTAILSGGFTYFARQLQDKLGIDYVFANELEVVDGKVTGVAVEPIVDAQRKADLLR
                     QLTEQEGLRLEQTIAVGDGANDLPMLAIAGLGVAFRAKPLVKQSAKQAISTLGLDGIL
                     YLLGYRDREGRD"
     gene            4717..>5590
                     /locus_tag="VIO83_03430"
     CDS             4717..>5590
                     /locus_tag="VIO83_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8330365.1"
                     /translation="MNRPAPVKPDNFFVLLLNALRRRRVPIALRIVSHSLLLVSLVVL
                     VCAWVLNMQLRQATQEQIEAVGQSLKQQAVASATELLVAKDLLSLNVMLDNLAQNPLV
                     AHVVIHSVDNRIIAEAGARPKQATLDRSEGVFSAPIAFQEIAAGQLQLTLDLEAFRKP
                     LTVSQQNLGLLGLIIAIVTLFSSLRLGSQLTTPLLQLRLWLRAPDDPAPANERQDEIG
                     DLARQLQNRLVPVKPAPAEPELEAREEDDEVLFEGLDNLLDERRPAPARVTPAAAADA
                     GARHAEPDFAADFAG"
BASE COUNT          966 a         2048 c         1751 g          825 t
ORIGIN      
        1 ttgcgcgcgc tgcaaccagc tgcgccagca ggccatccag ctgctggccg aagccctgcc
       61 ggcctggagc aaccgcgtcc gcctgcagga tgcccgcctg cgcgacagcc tgttcgcctt
      121 cgccccgggc atcgacagcc cgccgcgcta tgccagcctg ctgcaggacg aaggccacgc
      181 ccgcctgctc ggcctggccc cgcaaggcct ggtcgccgac ctgcgcgaat acctgctgct
      241 tggccgcggc cagcgcgtcc acgccaatct gccggtgccc tacggcatca ccgacgagct
      301 gctgcagcac ctgtgcacga cctggagcgc ccccgccgaa cgggcctaca accgcacacc
      361 cgccaacggc agcctgagcg cctgcctcgg catcagcgcc ctgcactact tcgtcgccgg
      421 gcatcgcagc ttcgccgagg tgctgcagct gccggacgag gaagtgaccg tcgacttcca
      481 gcggcgccag cgcgagcagg atacctgggc gcgcgccttt gacgccggcc acaacgaggc
      541 cctgctgcac aaggaacgcg aacggatcga cttcaccccg gtcggcaagc aggcggcagc
      601 gccagcgccc gaacgcgagg actacccgcg ccaccagctg cagatcgtca accagagccc
      661 cggcggctac tgcctggcgt gggacgacag cccccccagc cagctgcaga gcggtgacct
      721 ggtcggcctg caacaccccg gcgagcgcgg ctggaacatc gccctgatcc gctggatccg
      781 ccagccgcac ggtgccggcc tgcagctggg catcgagctg ctcgccccgc gcgcgcatcc
      841 ctgcggcctg cgcccgcaac cccgcagcgg gcagcctggc cagtacctgc gcggcctgct
      901 gctcagcgcg attccggcgc tgggccgacc ggccaccctg atcgccccgc acctgccgtt
      961 ccagcagggc cagaagatcc tgctcaacct cgatggcgaa gtgcagcgca tcctgctcac
     1021 ccaccggcat gccctgagcg acagtttcaa ccagttcgaa tatcgccggc tggacgcccg
     1081 cgtcgacctc gagcggaaac cggtcacagg attgagcatc gtcagcgaag agccggcgga
     1141 aggctttgac tcgctttggg agtccttgta gatttccgcc aaactccctt cctgatgcat
     1201 cttttgcgcc tgtttggcgc tgctccctgg cgatcaatgt ccaacgaaaa gaaaaccatc
     1261 cgcctgctga tcctcgagga ctcgcagaac gaggccgagc gactgatcag cctgtttcgc
     1321 aatgccggtc acgccacccg ggtgcatcgc ctgacctccg gcgacgatct cgccgacgcg
     1381 ctgcaacaga gctgggatct gctgatcgcc gccccgcaca gcgagaacct cgaccccggc
     1441 gaagcgctga gcgccatccg ccgccagggc aaggacatcc ccttcctgca acgggtcgcc
     1501 gacagcgaca gcgaggcgat caccgaagcc atgctgctcg gcgcccagga cggcgtgccg
     1561 cagggcgatg acgagcgcct gctgctggtc gccggacgcg agctgaagaa tctcgaggag
     1621 cgccgcgccc gccgtgccgc cgaagtggca ctgcgcgagg cggaaaagcg ctgccagctg
     1681 ctactcgaca gctcggtgga cgccatcacc tacgtgcacg aaggcatgca catctatgcc
     1741 aaccgcgcct atgtcgaact gttcggctat cccgatgccg acgacctggc cggcatgccg
     1801 atgatcgacc tgatccacag cggcgaccag gccgcgttca aggacttcct caagacctac
     1861 cagagcagcg agggccacgc cgacctgcag tgcagcggcg tacgcgacga cggcagccag
     1921 ttccgcgcgc gcatgacctt ctctccggcc acctacgacg gcgagccgtg cacccaggtg
     1981 gtgatccgcg cggaaaccgg caacgccgag ctggaggaaa agctgcgcga gatcagcagc
     2041 caggacctgg tcaccggcct gttcaaccgc gtgcacttcc tcgaactgct cgaccaggcc
     2101 tgcgagcggg cggtcaagga cggcagcgcg gccaccctcg ccctgctgcg catcgaccgc
     2161 tacgccgccc tgctcagcga gatcggcctg gccgacggcg acctgctgct ggccgactgc
     2221 gccaatctgc tgcgccagca tttcggcgat cgcgcccagc tggcgcgcct cgccgacgac
     2281 agctttgccg ccctgctgcc gacgctcacc ccgcagcaga gcgaagaagc gctgcacggc
     2341 ctgctgaaga aaatcgaagg ccatctgttc gacatcggcg gccgcaccac ccagatcacc
     2401 ctgagcatcg gcgtggccgg catcagcgag cagagtgcca agtcccagca ggtgatcgag
     2461 cgtgtccatc gctgcgtcga acaactgagc aacggcaacg ccctcaaact ctacgacccc
     2521 gccgaggagc tggccgccgc cgccagccgc ggcagcctgg tggccatggt ccagcaggcg
     2581 ctggacaaca acagcttcca gctgctgttc cagccgatca tcagcctgca cggcgacgag
     2641 cgcgagaact acgaggtcct gttgcgcctc agcaacccgc agggcgagcc ggtggccccg
     2701 gcggagttcc tcgccgcagc ccagcaggcc ggcctggccg agaaggtcga ccgctgggtg
     2761 ctgctcaaca gcatccgcca gctcggcgaa cagcgcgcca agggcggcaa cacccgcctg
     2821 ttcatccccc tcggcgccgc cagcctgcag gatccggtgc tgctgccctg gctcagcgcc
     2881 gcactcaagg ccgcccgcct gccagccgac gccctggtcc tgcagttctg cgagccggat
     2941 gcggtggcct acctcaagca ggccaagacc ctgagccagg gcctggccga gctgcactgc
     3001 aaggtcgccc tcagccagtt cggcggcgcg ctcaacccgt tcaacaccct gcgccacctg
     3061 catgtcgact tcgtcaagat cgacggctcg ctcatcgaca acctggcgga ggccgagcgc
     3121 caggagcagc tcaagagcct gatcggcggc ctgcacgcgc aagccaagct gaccatcgtg
     3181 cccggcgtgg acaaccccag cgtgctgacc accctgtggc agaccggggt caactacgtg
     3241 cagggcctgt acctgcaggg gccgagcccg cgcatggact acgagttctc ctccggcgag
     3301 gagtgagttt tccaacatca ggaataaaaa aaccgcctat tcggcggttt ttttattcct
     3361 ggaaacggtc agtcacgccc ctcgcgatcg cgataaccca gcagatagag gatgccgtcg
     3421 aggccaaggg tggagatcgc ctgcttggcc gactgcttga ccagcggctt ggcgcggaac
     3481 gccacgccga ggccggcgat cgccagcatc ggcagatcgt tggcgccgtc gccgacggcg
     3541 atggtctgct ccaggcgcag cccttcctgc tcggtcagct ggcgcaggag gtcggccttg
     3601 cgctgggcat cgacgatcgg ctccaccgcc acgccggtga ccttgccgtc gacgacttcc
     3661 agctcgttgg cgaacacgta gtcgatgccg agcttgtcct gcaactggcg ggcgaagtag
     3721 gtgaagccgc cggagaggat ggcggtcttg tagcccaggc gcttgagctc ggcgaacagg
     3781 aattcggcgc cttcggtcat gcgcaggccg gcaccgatgt cggccagcac ctcctcggac
     3841 agccccttga gcagcgccag gcgctccttg aagctggcgc gaaaatccag ctcgccgcgc
     3901 atcgcgcgct cggtgatttc ggcgacctgc tcgccgaccc cggcggcctt ggccagctcg
     3961 tcgatcacct ccgcctcgat cagcgtcgag tccatgtcga acaccgccag gcgacggttg
     4021 cgccggaaaa tcgtgtcctg ctggaaggcg atgtcgacgt tgagttcctg ggccacgctg
     4081 aggaactcgg cgcgcagcgc cgccgggtcg gccggctcgc cgcgcacgga aaactcgatg
     4141 cagcccttgc cgcgctcgtc cggggtatcc agcggcatgc gcccggacag gcggtcgatg
     4201 tggtcgatgt tcagcccgta ctgcgcggtg atcgagctga cccgctgcag ctgctcggcc
     4261 gtcaccttgc gggtcagcag ggtgacgatg tggcgcggct tgccctggcc gccgacccac
     4321 tgctgatagt cggcctcgga caccggggtg aagcgcacct gctgatccag ctcgtagccg
     4381 gtgaacagca ggtccttgag caccgacgac gccttggcgc tgtgcgggat ctcgaccagg
     4441 atgccgaacg acagggtgtt gtggatcacc gcctggccga tgtcgagaat gttcaccccg
     4501 ccacgcgcga ggacgcccgt gatggcggcg gtcaagcccg ggcgatcttc cccggtaatg
     4561 ttgatcagga cgatttcgcg cacggcgcac ctccccggaa ctgaaaagtt cggcattcta
     4621 cccgcaaagt gcaccatctg gcagcggcgc tttgccggat gcccgacgcc cccgctatac
     4681 ttcaactccc ctattcccag agccgagccc ccactcgtga accggcccgc gcccgtcaag
     4741 cccgacaact tcttcgtcct cctgctcaac gccctgcgcc ggcggcgggt gcccattgcc
     4801 ctgcgcatcg tcagccacag cctgctgctg gtcagcctgg tggtgctggt gtgcgcctgg
     4861 gtcctgaaca tgcagctgcg ccaagccacc caggaacaga tcgaagccgt cggccagagc
     4921 ctcaagcagc aggccgtcgc ctcggccacc gagctgctgg tggccaagga tctgctcagc
     4981 ctcaacgtga tgctcgacaa cctggcgcag aatccgctgg tcgcccacgt ggtgatccac
     5041 agcgtcgaca atcggatcat cgccgaggct ggcgcacgcc cgaagcaggc cactctggac
     5101 cgcagcgaag gggtgttcag tgcgccgatc gccttccagg aaatcgccgc cggccagctg
     5161 caactgaccc tcgacctcga ggcgttccgc aagccgctga ccgtcagcca gcagaacctc
     5221 ggtctgctcg ggctgatcat cgccatcgtc accctgttct ccagcctgcg tctgggcagc
     5281 cagctgacca ccccgctgct gcagctgcgc ctatggctgc gggccccgga tgacccggcg
     5341 ccggccaacg agcgccagga cgagatcggc gacctggcac gccagctgca aaaccgcctg
     5401 gtcccggtaa agcccgcgcc tgcggagcct gaactcgagg cgcgggaaga ggacgacgag
     5461 gtgctgttcg aggggctgga caacctgctc gacgaacgcc gcccggcgcc cgcccgcgtc
     5521 accccagcgg cagcagcgga cgctggcgcg cgccatgccg aacccgactt cgcggcggat
     5581 ttcgccggcg
//
LOCUS       DATCUN010000088         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_210259, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000088 DATCUN010000000
VERSION     DATCUN010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210259"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /gene="glnT"
                     /locus_tag="VIO83_03435"
     CDS             complement(<1..1229)
                     /gene="glnT"
                     /locus_tag="VIO83_03435"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009394916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HEY8330366.1"
                     /translation="MLPSETQKIIDQHGIKYVLAQFVDIHGAAKTKSVPICGLEAVAE
                     AGAGFAGFAICGMGMEPHGPDFMAKGDLSTLTPVPWQPGYGRVVCIGHVDGQPHPYDS
                     RYVLQQQVQRLTDKGWTLNTGLEPEFSLFQRDGAGKLQLVDASDNLDKPCYDYKGLSR
                     SRVFLERLTEALQPVGFEIYQIDHEDANGQFEINYTYSDAMTSADRFTFFRMAAGEIA
                     NELGMICSFMPKPDPKRAGNGMHFHLSISSATSKNLFHDASDPSGMGLSKMAYHFMAG
                     LLAHGPALCAFAAPTVNSYKRLVVGRSLSGATWAPAFITYGANNRSAMVRIPYGRIEF
                     RLPDAGCNPYLVSAAIIAAGLDGIDRQLEPGAVCNENLYNLSLEEIAGRGIATLPQSL
                     KEACDALEADPLFREVLG"
     gene            1482..>1819
                     /locus_tag="VIO83_03440"
     CDS             1482..>1819
                     /locus_tag="VIO83_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020291089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8330367.1"
                     /translation="MRKSDDNQPRLKLEQYLGMQIKRQRQAQELKIADVARIAGISQG
                     MLSKIENAQVSTSLDTLSRLCDVLGMPMSKLFSQYDQQGSGALLVKAGEGLEVVRRGT
                     DKGHTYHLLN"
BASE COUNT          324 a          579 c          604 g          312 t
ORIGIN      
        1 ccgagcacct cgcggaacag cgggtcggcc tccagcgcgt cgcaggcttc cttgaggctc
       61 tgcggcaggg tggcgatgcc gcgaccggcg atctcctcca ggctcaggtt gtagaggttc
      121 tcgttgcaca ccgcgcccgg ctcgagctgg cggtcgatgc cgtcgaggcc ggcggcgatg
      181 atcgcggcgc tgaccaggta cgggttgcaa ccggcgtccg gcagacggaa ctcgatgcgg
      241 ccgtagggga tgcgcaccat cgccgagcgg ttgttggcgc cgtaggtaat gaaggccggc
      301 gcccaggtcg cgccggacag cgagcggccc accaccaggc gcttgtagga gttcaccgtc
      361 ggcgcggcga aggcgcacag cgccgggccg tgggccagca ggccggccat gaagtggtag
      421 gccatcttcg acaggcccat gccgctcggg tcgctggcgt catggaacag gttcttgctg
      481 gtggcgctgc tgatcgacag gtggaagtgc atgccgttgc cggcgcgctt ggggtccggc
      541 ttgggcatga acgagcagat catccccagc tcgttggcga tctcgccggc ggccatgcgg
      601 aagaaggtga agcggtccgc cgaggtcatg gcgtcgctgt aggtgtagtt gatctcgaac
      661 tggccgttgg cgtcctcgtg gtcgatctgg tagatctcga agccgaccgg ctgcagcgcc
      721 tcggtcagcc gttccaggaa cacgcgggag cgcgacaggc ccttgtagtc gtagcagggc
      781 ttgtcgaggt tgtccgaggc atccaccagt tgcagcttgc cggcgccgtc gcgctggaac
      841 aggctgaact cgggctccag gccggtgttg agggtccagc ccttgtcggt caggcgctgc
      901 acctgctgct gcagaacata gcggctgtcg tagggatgcg gctggccgtc gacgtggccg
      961 atgcacacca cccggccgta gcccggctgc cagggcaccg gggtcagggt cgacaggtcg
     1021 cccttggcca tgaagtccgg gccgtggggc tccatgccca tgccgcagat ggcgaagccg
     1081 gcaaagccgg cgccggcctc ggcgaccgcc tccaggccgc agatcggcac cgacttggtt
     1141 ttggcggcgc catggatgtc gacaaactgc gcgagcacat acttgatgcc gtgctggtcg
     1201 atgattttct gggtttcgct gggcaacatg ggcggttggc tcctcggtcg ctgatgcttt
     1261 attccgctgg ggaaatttac tttcaccaaa agaaatgcaa acaccttgcc aaccttgcct
     1321 gcccctcgcc aacccggcga aagccccgca atccttctat ctatatggga aaaatggttt
     1381 catacgggga aatcgacgcg ccaggacggc cccgctcgtc cccgacccga tcattcttgg
     1441 tgcgaatctt atattcatga actgaaattg tgcaggccag catgagaaaa tccgacgaca
     1501 accagccgcg cctcaagctg gagcagtacc tgggcatgca gatcaagcgc cagcgccagg
     1561 cccaggaact gaagatcgcc gacgtcgcgc gcatcgccgg catcagccag ggcatgctca
     1621 gcaagatcga gaacgcccag gtctccacca gcctcgacac cctcagccgc ctgtgcgacg
     1681 tgctcggcat gccgatgtcc aaactgttca gccagtacga ccaacaaggc agcggcgcgc
     1741 tgctggtcaa ggccggcgaa ggcctcgaag tggtccgccg cggcaccgac aagggccaca
     1801 cctaccacct gctcaacca
//
LOCUS       DATCUN010000089        12293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_59821, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000089 DATCUN010000000
VERSION     DATCUN010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 12293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12293
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59821"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VIO83_03445"
     CDS             <1..251
                     /locus_tag="VIO83_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8330368.1"
                     /translation="KLKGSDVVGIKVNGHTDSTGTDAYNQGLSERRAASVVDYLVSQG
                     VAQDKLSSEGFGESKPVADNSSKEGRAENRRVEIMVDR"
     gene            387..740
                     /locus_tag="VIO83_03450"
     CDS             387..740
                     /locus_tag="VIO83_03450"
                     /inference="COORDINATES: protein motif:HMM:NF018334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 domain-containing protein"
                     /protein_id="HEY8330369.1"
                     /translation="MKLLTTFGRLLAVLFWLAVLGNLISPFAAPFALLVNLAATAVLL
                     LHVLQLAQCHGRLKVSAQPTRDRLLLLLFGSFYLQALPSPTSVVEQLLQATRVAEGDG
                     VTEPAPDAADQRSAA"
     gene            845..2989
                     /gene="dinG"
                     /locus_tag="VIO83_03455"
     CDS             845..2989
                     /gene="dinG"
                     /locus_tag="VIO83_03455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEY8330370.1"
                     /translation="MLSTELKSQIQGAYTRFLEAKSLKPRYGQRLMIAEVAKGLGAIA
                     TDDEGKRLGDPAVVAVEAGTGTGKTVAYALAAIPAAKAAGKRLVIATATVALQEQIVF
                     KDLPDLARNSGLNFTFALAKGRGRYLCLSKLDLLLQEGEAQGATAQLFEEEGFKIDVD
                     EASHKLFTQMIERLAGSRWDGDRDAWPEAIEDVQWSRLTTDHTQCTGRHCPNFQQCAF
                     YKAREGMTKVDVIVTNHDLVLADLALGGGAILPAPSETLYVFDEGHHLPDKAIGHFAH
                     FTRLRSTAEWLEQIAKNLTRLLAQNPLPGEFGRLIEGVPELARELRTQQQFVYAACEE
                     LGDFRPGEDMEGRDRPRHRFVGGLVPEHLREMGIELKKGFNRLNDVFTRLADILKEAM
                     DGDGSGGVSSYQAEEWYPLFGSLLARAQSNWELWTAFTLEDPEDSPPMARWLTLAESG
                     SYHDIEVNASPILAAETLRRNLWNVAYGALVTSATLTALGTFDRYRMRAGLPKGAVTA
                     VVPSPFRYAEAGLLRVPDLKADPRDAAAHTAAIIRELPAMVEGARGSLVLFSSRKQMQ
                     DVFDGLERDWRKRVLIQGNLSKQETLNKHKARVDDGETSVLFGLASFAEGVDLPGAYC
                     EHVLIAKIPFAVPDDPVEAALSEWIEARGGNPFMEIAVPDASLRLIQACGRLLRTEQD
                     TGTVTLLDRRVVTQRYGKAILSALPPFRRQID"
     gene            3110..4255
                     /locus_tag="VIO83_03460"
     CDS             3110..4255
                     /locus_tag="VIO83_03460"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017336808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY8330371.1"
                     /translation="MHIHGYFDLQFETVKEAFAALFNDPQERGAALCVQVGGETVLDL
                     WAGMADKDGLEPWHTDTILNLYSCTKAFTAAAALQLVGEGKLELDAPVTRLWPEFGAA
                     GKQHISLRQLLSHRAGLPAIRQSLPPEALYEWDTMAAALAAEAPWWTPGEAHGYAPIT
                     YGWLIGELIRRASGRSPGEVIVERTARPLGLDFHIGLADSEFHRVAHISRGKGNLGDA
                     AAQRVLNAMLREPQAMTTRAFTNPPSIMTSTNKPEWRRMQQPAANGHGNARGLAGFYA
                     GLLDGSLLDSALLAEMTREHSLGDDLTLLTRTRLGLGLMLEQPDMPNATYGLGRHAFG
                     HPGAGGSIGFADPERELAFGFVTNTLGPYVLMDPRAQRLARLVGECL"
     gene            complement(4523..4599)
                     /locus_tag="VIO83_03465"
     tRNA            complement(4523..4599)
                     /locus_tag="VIO83_03465"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4563..4565),aa:Pro,seq:cgg)
     gene            4774..5313
                     /gene="moaB"
                     /locus_tag="VIO83_03470"
     CDS             4774..5313
                     /gene="moaB"
                     /locus_tag="VIO83_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEY8330372.1"
                     /translation="MSQHAESVFVPLNIAVLTVSDTRTLDTDTSGGLLVERLQAAGHS
                     LAARVLLKDDLYRIRAQVAQWIAEDEVQVVLITGGTGFTGRDSTPEAVACLLDKQVDG
                     FGELFRQISVPDIGTSTIQSRALAGLANGTLVCCLPGSTNACRTGWDGILATQLDARH
                     RPCNFVTHLKQAPACGTRG"
     gene            5310..6506
                     /gene="glp"
                     /locus_tag="VIO83_03475"
     CDS             5310..6506
                     /gene="glp"
                     /locus_tag="VIO83_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY8330373.1"
                     /translation="MSALLPLEEALARLLAQAAVEPIVETQCIPAGEADGRVLATALV
                     AGLDLPPWPNSAMDGYALSLADWQGEPLAVSQQIFAGQAPVPLQPGTCARIFTGAPLP
                     AGADSVEMQENVEVLADGRVHFLEALRPGQNVRPQGQEIRRGEVLLETGTRLGPVELG
                     LIASVGVAQVEVRRRPRVALLSTGDELVEPGQPLGAGQIYNSNRALLAAWLTRLGCAV
                     LDAGILPDDLAQTRARLAELGEADLILSTGGVSVGEADFLGQVLREEGELTLWKLALK
                     PGKPLTCGHFRGTPLIGLPGNPASTLVTFALLARPYLLRRLGVSRLEPLRFAVPAAFA
                     WPKAGGRREFLRARLEQGRAVLHANQSSGVLRSAAWADGLVEIREGSTLVEGEAVTFI
                     PLAELF"
     gene            6610..7686
                     /locus_tag="VIO83_03480"
     CDS             6610..7686
                     /locus_tag="VIO83_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY8330374.1"
                     /translation="MTMMKAAVFVAPGRIELVDKPIPPVGPNDALVRITTTTICGTDV
                     HILKGEYPVAPGLTIGHEPVGIIEKLGSNVQGYQEGQRVIAGAICPSFTSYACQDGYP
                     SQDGGCACHGYKPMGGWRFGNTIDGTQAEYVLVPDAQANLAPVPDGLSDEQVLMCPDI
                     MSTGFAGAEAANIKIGDIVVVFAQGPIGLCATAGAKLRGASTIIAVDGVNERLDIARQ
                     LGADVTLNFREVDVVDEILKLTGGRGVDAAIEALGTQATFESALRVLKPGGTLSSLGV
                     YSSDLVIPLGAFHAGLGDNKIVTSLCPGGKERMRRLLNVVASGRVDLGLLVTHEYKLD
                     DIVAAYDLFGHQRDGVLKVAIKPF"
     gene            7825..9306
                     /gene="gltX"
                     /locus_tag="VIO83_03485"
     CDS             7825..9306
                     /gene="gltX"
                     /locus_tag="VIO83_03485"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY8330375.1"
                     /translation="MTTVRTRIAPSPTGDPHVGTAYIALFNLCFARQHGGQFILRIED
                     TDQLRSTRESEQQIYDALRWLGIEWDEGPDVGGPHGPYRQSERGHIYKQYSDELVAKG
                     HAFPCFCSAERLDAVRAEQMANKETPRYDGHCMHIDPVEAQQRVAAGEAHVVRMKVPT
                     EGVCVVPDMLRGEVEIPWDRMDMQVLMKTDGLPTYFLANVVDDHLMGITHVLRGEEWL
                     PSAPKLIKLYEYFGWEQPVLCYMPLLRNPDKSKLSKRKNPTSVTFYQRMGYLPEAMLN
                     YLGRMGWSMPDEREKFSLAEMIEHFDVSRVSLGGPIFDVDKLSWLNGQWLRELPLERF
                     AAEVQKWALNPQYLMQIAPHVQNRVETFSQLAPLAGFFFMGGVSPDAKLFEHKKLSPE
                     QVRQVLQLILWKLEALRQWNKDNITAIIQKVAEHLELKLRDLMPLIFPAISGQASSVS
                     VLDAMEILGPDLSRFRLRQAVELLGGVSKKETKEWEKLLAAIE"
     gene            9483..9558
                     /locus_tag="VIO83_03490"
     tRNA            9483..9558
                     /locus_tag="VIO83_03490"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9516..9518,aa:Ala,seq:ggc)
     gene            9607..9682
                     /locus_tag="VIO83_03495"
     tRNA            9607..9682
                     /locus_tag="VIO83_03495"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9641..9643,aa:Glu,seq:ttc)
     gene            9727..9802
                     /locus_tag="VIO83_03500"
     tRNA            9727..9802
                     /locus_tag="VIO83_03500"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9760..9762,aa:Ala,seq:ggc)
     gene            9842..9917
                     /locus_tag="VIO83_03505"
     tRNA            9842..9917
                     /locus_tag="VIO83_03505"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9876..9878,aa:Glu,seq:ttc)
     gene            10180..10587
                     /locus_tag="VIO83_03510"
     CDS             10180..10587
                     /locus_tag="VIO83_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEY8330376.1"
                     /translation="MTFMPWSSELEVGIPQIDEQHRWLVDKTNQLYSSLASAEADGAA
                     IGEVLEGLMDYTMNHFIVEEEIFHRLGYPETAAHKAQHDKFTEQIMDLLQRHDAGERS
                     GNETLDLLADWLVHHILKVDKAYVAFFRENGVR"
     gene            10658..11341
                     /locus_tag="VIO83_03515"
     CDS             10658..11341
                     /locus_tag="VIO83_03515"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY8330377.1"
                     /translation="MNAPVLCLYPDRDTCAAELAQVLGARLRAEQSAGGRARLLLAGG
                     QSPLPLLHRLAGETLDWSRVDLSPTDERWVAAADPASNLRMLCSALPQAQALDPRQAA
                     IPQVAAQAWGEQLAGWLPLTAVLLGMGEDGHFASLFPGMPGLSAALAADASAGALVGV
                     APVEPCLRLTPNLVLLLRSGWLGLLVFGSAKRELLEAVLADRPDTRDLPVHALVQQAG
                     SRLQIHWAP"
     gene            11473..>12293
                     /locus_tag="VIO83_03520"
     CDS             11473..>12293
                     /locus_tag="VIO83_03520"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY8330378.1"
                     /translation="MHPVVEAVTARIVARSAERRARYLARVDEAAAREPRREVGCSNL
                     AHALAAQPYDTQLIMRQGGSPHIAIVSSYNDLLSAHQPLHDYPEQLKAALARHGASAQ
                     FAAGVPAMCDGITQGMAGMQLSLFSRDLIAQAAAIGLTHAIFDGGLYLGVCDKIVPGL
                     LIGALRFGHLPAVFVPAGPMPSGLSNSAKAAVRQRYARGEASREELLEAELGAYHAPG
                     TCTFYGTANTNQLLMEAMGLHVPGSAFAPPESALRPLLTAEAARLVARNSRQGER"
BASE COUNT         1975 a         4127 c         4153 g         2038 t
ORIGIN      
        1 agaagctcaa gggcagcgat gtggtcggca tcaaggtgaa tggccacacc gacagcaccg
       61 gtaccgatgc ctataaccag gggctctcgg agcggcgcgc cgccagcgtg gtggactatc
      121 tggtcagcca gggcgtggcg caggacaagc tgagttccga aggctttggc gagagcaagc
      181 cggtggcgga caacagcagc aaggaagggc gggcggagaa ccgtcgggtg gagatcatgg
      241 tcgaccgcta gcgctccgac ggtggtcatg cgtttgtcgc catgaggggc cggttggccc
      301 cctcatggct gtctgcacgt cggcacaccc ttggcagccc tggcggctgc cgttaaggtg
      361 cgcgctgatt cctctcggat acctcgatga aactgctgac gacctttggc cgcctgctgg
      421 ccgtgctgtt ctggctggcg gtgctgggca acctgatttc tcccttcgct gcgcccttcg
      481 ccttgctggt caatctggct gctacggcag tgctgctgct gcatgtcctg cagctggcgc
      541 agtgccatgg tcgactcaag gtcagtgcgc agccgacccg cgaccgcctg ctgctgctcc
      601 tgttcggctc gttttatctg caggccctgc catcgcccac gtcggtggtc gagcagctgc
      661 tgcaggcgac ccgcgtggcg gagggcgatg gggtgaccga gccggcgcct gacgccgcgg
      721 atcagcgcag cgccgcctga gtcccagccg gggcctgccg gtcgatcctt tctgcaggcg
      781 ggcggccagc gcgttatcat ggccgcccgt cccgatcact gaacgtttat ccagcgcgcc
      841 cgccatgctc agcaccgaac tcaagtccca gatccagggc gcctacaccc gctttctcga
      901 ggccaagagc ctcaagccgc gctacggcca gcgcctgatg atcgccgaag tggccaaggg
      961 cctcggcgcc atcgccaccg acgacgaggg caagcgcctg ggcgatccgg ctgtggtggc
     1021 ggtggaagcc ggcaccggta ccggcaagac cgtggcctac gccctggcgg cgattcccgc
     1081 ggccaaggct gccggcaagc gcctggtgat cgccaccgcc acggtcgccc tgcaggagca
     1141 gatcgtcttc aaggacctgc cggacctggc gcgcaacagc gggctgaact tcaccttcgc
     1201 cctggccaag ggccgcggcc gctacctgtg cctgtccaag ctcgacctgc tgctgcagga
     1261 gggcgaggcg cagggtgcca cggcccagtt gttcgaggaa gaaggcttca agatcgacgt
     1321 cgacgaggcc agccacaagc tgttcaccca gatgatcgag cgcctggccg gcagtcgctg
     1381 ggacggcgac cgcgacgcct ggccggaagc catcgaggat gtgcagtggt cgcggctgac
     1441 caccgaccac acccagtgca ccggccgcca ctgcccgaac ttccagcagt gcgccttcta
     1501 caaggcgcgc gagggcatga ccaaggtcga cgtgatcgtc accaaccacg acctggtgct
     1561 ggccgacctg gccctcggcg gcggcgccat cctgccggcg ccgagcgaaa ccctctacgt
     1621 gttcgacgaa ggccaccacc tgccggacaa ggccatcggc cacttcgccc acttcacccg
     1681 cctgcgctcc accgccgagt ggctggagca gatcgccaag aacctcaccc gcctgctggc
     1741 gcagaacccg ctgcccggcg agttcggccg gctgatcgaa ggcgtgccgg aactggcccg
     1801 cgagctccgc acccagcagc agttcgtcta cgccgcctgc gaggaactcg gcgacttccg
     1861 tcccggcgag gacatggaag gccgcgaccg gccgcggcac cgcttcgtcg gtgggctggt
     1921 gcccgagcac ctgcgcgaga tgggcatcga gctgaagaag ggtttcaacc gcctgaacga
     1981 cgtgttcacc cgtctggccg acatcctcaa ggaggccatg gatggtgacg gcagcggcgg
     2041 ggtgagtagc taccaggccg aggagtggta cccgctgttc ggcagcctgc tggcgcgggc
     2101 gcagagcaac tgggagctgt ggaccgcctt caccctcgag gatcccgagg acagcccgcc
     2161 gatggcgcgc tggttgaccc tggccgagag cggcagctat cacgacatcg aggtcaacgc
     2221 cagcccgatc ctcgctgccg aaaccctgcg ccgcaacctg tggaacgtcg cctatggcgc
     2281 cctggtgacc tcggccacgc tcaccgcgct gggcaccttc gaccgctacc gcatgcgtgc
     2341 cggcttgccc aagggggcgg tcaccgccgt ggtgcccagt ccgttccgct acgccgaggc
     2401 cggcctcttg cgcgtgcccg acctcaaggc cgacccgcgc gacgccgctg cgcatactgc
     2461 ggcgatcatc cgcgagctgc cggccatggt cgaaggcgcg cgcggcagcc tggtgctgtt
     2521 ctcctcgcgc aagcagatgc aggacgtgtt cgacggcctc gagcgcgact ggcgcaagcg
     2581 cgtgctgatc cagggcaacc tgtccaagca ggagacgctg aacaagcaca aggcgcgggt
     2641 cgacgacggc gagaccagcg tgctgttcgg cctggccagc ttcgccgagg gcgtcgacct
     2701 gcccggcgcc tactgcgagc acgtgctgat cgccaagatc cccttcgcgg tgccggacga
     2761 cccggtggaa gcggcactgt ccgagtggat cgaggcacgc ggcggcaacc cgttcatgga
     2821 gatcgccgtg cccgacgcct ccttgcgcct gatccaggcc tgcggccgcc tgctgcgcac
     2881 cgagcaggac accggcacgg tgaccctgct agaccgccgc gtggtcaccc agcgctacgg
     2941 caaggccatc ctcagcgccc tgccgccgtt ccgccggcag atcgactgaa ctcatattgc
     3001 tggtgcgcgc ggcgcaccct acggcgcggc gcaatggtag ggtgcgccgt gcgccgtgcg
     3061 ccgcatggcc actgccgctc gcaaccaaca accaggaaca ggagtcccca tgcacatcca
     3121 cggctatttc gacctgcagt tcgagacggt gaaggaggcc ttcgccgcac tgttcaacga
     3181 cccgcaggag cgcggtgccg cactgtgcgt gcaggtcggc ggcgaaaccg tgctcgacct
     3241 gtgggccggc atggccgaca aggacggcct cgagccctgg cacaccgaca ccatcctcaa
     3301 cctgtattcg tgcaccaagg ccttcaccgc cgccgccgcc ctgcagctgg tgggcgaggg
     3361 caagctggag ctggacgcac cggtcacccg cctgtggccc gaattcggcg ccgccggcaa
     3421 gcagcacatc agcctgcgcc agctgctcag ccaccgcgcc ggcctgccgg cgatccgcca
     3481 gtcgctgccg ccggaagccc tctacgagtg ggacaccatg gccgctgcac tggctgccga
     3541 ggcgccctgg tggacgccgg gcgaggcgca cggctacgcg ccgatcacct acggctggct
     3601 gatcggcgaa ctgatccgcc gcgccagcgg gcgcagcccc ggcgaggtga tcgtcgagcg
     3661 cactgccagg ccgctgggcc tcgacttcca catcggcctg gccgacagcg agttccatcg
     3721 cgtcgcccac atctcccgtg gcaagggcaa cctcggcgac gccgccgccc agcgcgtgct
     3781 caacgcaatg ctgcgcgaac cgcaggcgat gaccacccgc gccttcacca acccgccgtc
     3841 gatcatgacc agcaccaaca agccggagtg gcgacgcatg cagcagccgg ccgccaacgg
     3901 ccacggcaac gcccgcggcc tcgccggctt ctatgccggc ctgctcgacg gcagcctgct
     3961 cgacagcgcc ctgctggccg agatgacccg cgagcacagc ctgggcgacg acctcaccct
     4021 gctgacccgc acccgcctgg ggctgggcct gatgctcgaa cagccggaca tgcccaacgc
     4081 cacctacggt ctcggccgcc atgccttcgg ccaccccggc gccggcggct ccatcggctt
     4141 tgccgacccc gagcgcgagc tggccttcgg cttcgtcacc aataccctcg ggccgtatgt
     4201 gctgatggac ccgcgcgcgc aaaggctggc acgtctggtg ggggagtgct tgtaggggcg
     4261 ttggtgggac gagggtgccc atagcaggta tgggcgctcc ccatgcccct ggatgggctg
     4321 ggcggcgccg ttcagggggc tgatctcggg ggcggcaggc tgtgcgcatc ccgtccgcgc
     4381 gaaggttgtc cggcggatat ggagagggag gcgcgcgggt cggtgccggg gagaggcggg
     4441 aggcatattg cagaaacaac aaggcccgca ctgggcgggc cttgttgggt gattcaccga
     4501 agttcatcgg cggatcggga tatggtcgga gcgactggat tcgaaccagc gaccttctcg
     4561 tcccgaacga gacgcgctac caagctgcgc tacgctccgt tttgatgggg ctcattttac
     4621 agaaaaaatg atggtgcaca aggggttagg tgaaaaaaat taaaaactgc tgcatcggga
     4681 gatgacgcgc tgtggcgtga gtcaggttgg cccggttggt gctataaaac cgatcaggtc
     4741 ttttttgtcc cgaacccagc gcggagcctc ttgatgagcc agcacgccga aagcgttttc
     4801 gtccccctga acattgccgt cctgacggtc agcgataccc gtaccctgga taccgacacc
     4861 tccggcggtt tgctggtcga gcgcctgcag gcggcgggtc atagcctggc ggcacgggtg
     4921 ctgctcaagg acgatctcta tcgcatccgt gcccaggtgg cgcagtggat cgccgaggac
     4981 gaggttcagg tggtgctgat caccggcggc accggcttca ccggtcgcga cagcaccccc
     5041 gaggcggtgg cctgcctgct ggacaagcag gtggacggct tcggcgaatt gttccggcag
     5101 atctcggtgc cggacatcgg cacctcgacc atccagtcgc gtgccctggc cgggctggcc
     5161 aacggcaccc tggtctgctg cctgccgggc tcgaccaatg cctgtcgcac cggctgggat
     5221 ggcatcctgg ccacccaact ggatgcccgg caccggccgt gcaacttcgt cacccatctg
     5281 aagcaggcgc cagcctgcgg gacgcgcgga tgagtgcgct gctgccgctg gaggaggcgc
     5341 tggcgcgcct gctggcgcag gccgccgtcg agccgatcgt cgagacgcag tgcatccccg
     5401 ccggcgaagc cgatgggcgg gtgctggcga cggcgctggt cgccgggctc gatctgccgc
     5461 cctggccgaa cagcgcgatg gatggctacg cgttgagtct ggccgactgg cagggcgagc
     5521 cgctggcggt cagccagcag atcttcgccg gccaggcacc cgtgccgctg cagccgggga
     5581 cctgtgcgcg catcttcacc ggcgcgccgc tgcccgcggg cgcggatagc gtggagatgc
     5641 aggagaacgt cgaggtgctg gccgacggtc gggtgcactt tctcgaggcg ctgcggcctg
     5701 gccagaacgt gcggccccag gggcaggaga tccgccgggg cgaagtgctg ctcgagaccg
     5761 gtacccgcct ggggccggtg gaactgggcc tgatcgcttc cgtcggcgtg gcgcaggtcg
     5821 aggtgcgccg ccggccacgg gtggccctgc tgtccactgg cgatgagctg gtcgagccgg
     5881 gccagccgct gggtgccgga cagatctaca acagcaatcg cgccctgctg gcggcctggc
     5941 tgacccgcct gggctgcgcg gtgctggatg ccggcatcct gcccgacgac ctggcgcaga
     6001 cccgtgcgcg tctggcagag ctgggcgagg cggacctgat cctttccacc ggcggggttt
     6061 cggtcggcga ggcggacttc ctcggccagg tgctgcgcga ggagggcgag ctgaccctgt
     6121 ggaagctggc gctcaagccg ggcaagccac tgacctgcgg tcatttccgc ggtacgccgc
     6181 tgatcggcct gcccggcaat ccggcttcca ccctggtgac cttcgccctg ctggcgcgcc
     6241 cctatctgtt gcgtcgtctt ggtgtcagcc gtctcgaacc gttgcgcttt gctgtcccgg
     6301 cggcttttgc ctggcccaag gccggtggcc gtcgcgagtt cctgcgtgcc cggctggagc
     6361 agggcagggc ggtgctgcat gcgaaccaga gctccggcgt gctgcgcagc gccgcctggg
     6421 ccgacgggct ggtcgagatc cgcgagggca gcacgctggt cgagggcgag gcggtgacct
     6481 tcattccgct ggcggagctg ttctgagcag gcgcatgacc tcgggcatgt tgttgtaacc
     6541 gtcgctcgac tagagtgggt gactaccccg gccgggttgc ccggctccat tggtgtaagg
     6601 agtattccga tgacgatgat gaaagctgcc gtattcgtgg cccccggccg catcgaactg
     6661 gtcgacaagc cgatcccgcc ggtggggccc aacgacgcgc tggtgcgcat caccaccacc
     6721 accatctgcg gcaccgacgt gcacatcctc aagggtgagt atccggtggc gccggggctg
     6781 accatcggcc acgaaccggt gggcatcatc gagaagctcg gcagcaacgt gcagggctat
     6841 caggaaggcc agcgggtgat cgccggggcg atctgtccga gcttcacctc ctacgcctgc
     6901 caggatggct atccgtcgca ggacggcggc tgcgcctgcc acggctacaa gccgatgggc
     6961 ggctggcgct tcggcaacac catcgacggc acccaggccg agtacgtgct ggtgcccgat
     7021 gcccaggcca acctcgcgcc ggtgccggac gggctgagcg acgaacaggt gctgatgtgc
     7081 ccggacatca tgtccaccgg ctttgccggt gccgaggcgg ccaacatcaa gatcggcgac
     7141 atcgtcgtgg tgttcgccca ggggcctatc ggcctgtgcg ccaccgccgg ggccaagctg
     7201 cgcggtgcca gcaccatcat tgccgtggat ggggtcaacg agcgtctgga catcgcccgc
     7261 cagttgggcg ccgacgtgac cctcaacttc cgcgaagtgg acgtggtcga cgaaatcctc
     7321 aagctgaccg gcggccgcgg cgtggatgcg gcgatcgagg cgctcggcac ccaggcgacc
     7381 ttcgagagcg cgctgcgcgt actcaagccg ggcggcaccc tgtccagcct gggcgtgtac
     7441 tccagcgacc tggtcattcc gctgggtgcc ttccatgccg gcctcggcga caacaagatc
     7501 gtcacctcgc tgtgcccggg cggcaaggag cgcatgcgcc gcctgctcaa tgtggtggct
     7561 tccgggcggg tcgacctggg tctgctggtg acccacgagt acaagctgga cgacatagtc
     7621 gcggcctacg acctgttcgg ccatcagcgt gacggcgtgc tcaaggtggc gatcaagccg
     7681 ttctgagggc tgccctgaag tcgacgaagc ccggccgcgt ccgggctttg tcgtttgtgg
     7741 tcggtgtggg gctgcggctg gagccggggg cggcgtggct gctaacatcg cgcctttgca
     7801 tcaacgctca atccgagagt cgctatgacc accgtccgta cccgcatcgc gccgtcgccc
     7861 accggcgacc cgcacgttgg caccgcctat atcgccctgt tcaacctgtg cttcgcccgc
     7921 cagcacggcg gtcagttcat cctgcgcatc gaggataccg accagctgcg ttcgacccgc
     7981 gagtccgaac agcagatcta cgacgccctg cgctggctgg gcatcgagtg ggacgagggc
     8041 ccggatgtcg gcggtccgca cggtccgtac cggcagagcg agcgcggcca catctacaag
     8101 cagtactcgg acgagctggt cgccaagggc cacgccttcc cctgcttctg cagcgccgag
     8161 cgcctcgacg cggtgcgtgc cgagcagatg gccaacaagg aaacaccgcg ctacgacggc
     8221 cactgcatgc acatcgatcc ggtcgaggcg cagcagcgcg ttgccgccgg cgaggcccat
     8281 gtggtgcgca tgaaggtgcc gaccgagggc gtctgcgtgg tgccggacat gttgcgcggc
     8341 gaggtggaga tcccctggga tcgcatggac atgcaggtgc tgatgaagac cgacggcctg
     8401 cccacctact tcctcgccaa cgtggtcgac gaccacctga tgggcatcac ccacgtgctg
     8461 cgtggcgagg agtggctgcc gtcggcgccc aagctgatca agctgtacga gtacttcggc
     8521 tgggaacagc cggtgctctg ctacatgccg ctgctgcgca atccggacaa gagcaagctg
     8581 tccaagcgca agaacccgac ctcggtgacc ttctaccagc gcatgggcta cctgccggaa
     8641 gccatgctca actacctggg gcgcatgggc tggtcgatgc cggacgagcg cgagaagttc
     8701 tccctggccg agatgatcga gcacttcgat gtcagccggg tgtcgctcgg cgggccgatc
     8761 ttcgacgtcg acaagctgtc ctggctgaac ggccagtggc tgcgcgagct gccgctggag
     8821 cgcttcgccg ccgaggtgca gaagtgggcg ctcaatccgc agtacctgat gcagatcgcc
     8881 ccgcacgtgc agaaccgcgt ggaaaccttc agccagctgg cgccgctggc cggcttcttc
     8941 ttcatgggcg gcgtgagccc ggacgccaag ctgttcgagc acaagaagct gtcgcccgaa
     9001 caggtgcgtc aggtgctgca gctgatcctc tggaagctgg aggcgctgcg ccagtggaac
     9061 aaggacaaca tcaccgcgat catccagaag gtcgccgagc acctcgagct caagctgcgt
     9121 gacctgatgc cgctgatctt cccggccatc agtggtcagg ccagctcggt gtcggtgctg
     9181 gacgccatgg agatcctcgg tccggacctg tcgcgcttcc gcctgcgcca ggcggtcgag
     9241 ctgctgggcg gcgtgtcgaa gaaggaaacc aaggagtggg agaagctgct cgctgccatc
     9301 gagtgagccg cagcggtggc ggggtatgcc ccgtcaccgc gcatccggtg cttaagtgat
     9361 tgttctgttg gataaatttt ttgaaaaaag tgcgtgtgag tgttgacagg gttccggttc
     9421 gcccttaaca tgcgccccgt cttcacgacg actagctcga aagtgaagcg atccacgaaa
     9481 gtggggctat agctcagctg ggagagcgct tgcatggcat gcaagaggtc gacggttcga
     9541 tcccgtctag ctccaccaaa ttatccagca acacgctgat gtctggcggg ttgtacgaag
     9601 gtttaagtcc ccttcgtcta gtggcctagg acaccgccct ttcacggcgg taacaggggt
     9661 tcgagtcccc taggggacgc caatttccag cgcagcgcca acaagggctg cagccgaagc
     9721 agtatcgggg ctatagctca gctgggagag cgcttgcatg gcatgcaaga ggtcgacggt
     9781 tcgatcccgt ctagctccac caatttccag cgattcgtcc gcgaatcgta cgaaggttta
     9841 agtccccttc gtctagtggc ctaggacacc gccctttcac ggcggtaaca ggggttcgag
     9901 tcccctaggg gacgccaatt ttcccgctct gggatcaaga gggtcattcc acttttggaa
     9961 tgaccctttt tgttttttcc gcgaaaaaat ccgctgcccg ctgtttttct cacgtctcac
    10021 gtctcacgtc tcacgctgcc ggctgtgcgg cgggagggtg ggcgcgtcat tcccctgtca
    10081 atcgttgtcc ataatttttg tgtgggataa gctggcgacg ccgagattgt ctggcagcca
    10141 ttcacataac aagacaacca gcaggggaag gacaaggaca tgactttcat gccgtggagc
    10201 agcgagctcg aagtcggaat tccgcagatc gacgaacagc atcgctggct ggtggacaag
    10261 accaaccagc tgtacagcag cctggccagc gccgaagcgg atggcgccgc gatcggcgag
    10321 gtgctggaag ggttgatgga ctacacgatg aatcacttca ttgtcgagga ggagatcttc
    10381 catcgcctcg gctatccgga aaccgcggcg cacaaggccc agcacgacaa gttcaccgag
    10441 cagatcatgg acctgctgca gcgccacgac gccggcgagc gctccggcaa cgagactctc
    10501 gacttgctgg ccgactggct ggttcaccat atcctcaagg tcgacaaggc ctacgtcgcc
    10561 ttctttcgcg aaaacggcgt gcgctgagct gtccggctgt tgcgcgcaag gctgaatgcc
    10621 gcatcatgct aggcattcct tgctgcggaa gtgccggatg aacgctcccg tcctttgtct
    10681 gtatcccgac cgcgacacct gcgctgccga gctggcgcag gtgctgggcg cgcgtctgcg
    10741 cgcggagcaa tcggccggcg ggcgtgcccg cctgctgctg gccggcggac agagcccgct
    10801 gccgctgctg caccggctgg ctggcgagac gctggactgg tcgcgggtcg atctttcccc
    10861 taccgacgag cgctgggtgg ccgccgccga tcctgccagc aacctgcgga tgctgtgcag
    10921 cgccctgccg caggcgcagg cactcgatcc gcgtcaggcc gcgatcccac aggtcgcggc
    10981 gcaggcctgg ggcgaacagc tggccggctg gctgccgctg accgccgtgc tgctgggcat
    11041 gggcgaggac ggccattttg cctcgctgtt tcccggcatg ccgggactta gcgctgccct
    11101 ggccgccgat gcgtcggcgg gcgcgctggt cggggtggcg cccgtcgagc cgtgcttgcg
    11161 gctgacgccc aatctggtgc tgctgctgcg tagcggttgg ctggggctgc tggtgttcgg
    11221 cagcgccaaa cgcgagctgc tcgaggccgt gttggccgat cggccggata cccgcgatct
    11281 gccggtgcac gcgctggtac agcaagccgg tagccgcttg cagatccact gggcgccttg
    11341 attcggtgag tgtgcaactg ctgcgctcgg ttattcgcgg tgctgataaa ctggcggcct
    11401 gatggagcct gtgcctgtcg cgcggcgaca gggtcgcgtt cccgcaaagc cttcagccga
    11461 ggagtcgcca tgctgcatcc cgttgtcgag gcggtgaccg cccgtattgt cgcccgctcc
    11521 gccgagcgcc gcgcgcgcta cctggcgcgt gtcgacgagg cggccgcccg cgagccgcgc
    11581 cgggaggtcg gctgctccaa tctggctcac gcgctggcgg ctcaaccgta tgatacgcag
    11641 ctgatcatgc gtcagggcgg ctcgccgcat atcgccatcg tctccagcta caacgacctg
    11701 ctctccgcgc accagccgct gcacgactac cccgagcagc tcaaggccgc cctggcccga
    11761 cacggcgcca gcgcgcagtt cgccgccggg gtgccggcca tgtgcgacgg catcacccag
    11821 ggcatggcgg gcatgcagct gtcgctgttc tcccgcgacc tgatcgccca ggccgccgcc
    11881 atcggcctga cccacgccat cttcgacggc ggcctgtatc tgggcgtgtg cgacaagatc
    11941 gtgccgggcc tgttgatcgg cgcgctgcgc ttcggccatc tgccggcggt gttcgtgccg
    12001 gccgggccga tgccgtccgg gctgagcaat agcgcgaagg cggcggtacg ccagcgttat
    12061 gcgcgcggcg aggccagtcg cgaggagctg ctcgaggccg agctgggcgc ctaccatgcg
    12121 ccgggcacct gcaccttcta cggcaccgcc aacaccaacc agctgctgat ggaggccatg
    12181 ggcctgcatg tgccaggcag cgccttcgcg ccgccggagt cggcgttgcg cccgctgctc
    12241 accgccgagg cggcgcgcct ggtggcgcgc aacagccgtc agggcgagcg ctg
//
LOCUS       DATCUN010000090         4209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_248354, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000090 DATCUN010000000
VERSION     DATCUN010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248354"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VIO83_03525"
     CDS             complement(<1..290)
                     /locus_tag="VIO83_03525"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8330379.1"
                     /translation="MDLEAWSARLKVFADATRVRLLALLASEELTVAELSSITRLAQP
                     RVSTHLAKLKDAGLVRDRRAGVSAYYRFDEDNLDPAQRDLWRSLRAGSDDPLL"
     gene            415..1563
                     /locus_tag="VIO83_03530"
     CDS             415..1563
                     /locus_tag="VIO83_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8330380.1"
                     /translation="MDFGYTEEQLMIQDVARRIAQEKIAPSAEHFDRTGEFPLENIRL
                     LGENGLMGIEVPAEYGGAGMDPVSYVLAMIEVAAGDAAHATIMSVNNSLYCSGLLKFA
                     TEEQKQLYVRAIADGSQIGAFALTEPQSGSDATAMRCRAVRQDDGTFVINGKKSWITS
                     GPVARYIVLFAMTAPDKGAKGITAFIVDTGREGFHRGKTEPKLGIRASATCEIEFADY
                     LAQPGEVVGGEGEGFRIAMGVLDAGRIGIASQAIGIARAAYEKTIEYVKERKAFGAPI
                     GTFQMTQAKIADMKCKLDASLLLTLRAAWLKGQGRKFGSEAAIAKLTASEAAMWITHQ
                     AVQIHGGMGYSKEMPLERYFRDAKITEIYEGTSEIQRLVIARGETGLR"
     gene            1860..3734
                     /locus_tag="VIO83_03535"
     CDS             1860..3734
                     /locus_tag="VIO83_03535"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY8330381.1"
                     /translation="MRQTLIACALALALTGPAGATTPQAGAAWTPPAAISQADIKAET
                     ERLNQWFEKKYEEQLRFSPLQLTFQGRKDLYDQLDDLSEQATLDQVAWQKSSVEEMEK
                     TFAYAKLSDEGKFSYDLWKLQYENARDGLPFLVDRYAFDQMKGAQSFLPTILINFHKV
                     DEEADYSAYVARLAATARAFDQLLERARKSARQGVRPPKFAYEGVIDQARKVIAGAPF
                     SEGEDSSIWADAQAKADALVKNGKIDAERAASLKQQARTVLLEQFKPGYERIIAWCEQ
                     DLPNAAVNASGVGTTHANGKAYYQYQLRQMTTTNMTADQIHALGLKEVERIKGEMNAL
                     KNRVGFKGDLDAFFAFIDSDPQFKFPNTDAGREAYIDQATRVIANIKAQLPNYFGLLP
                     KADLVVKRVEAFREQPGAAQHYFPGTPDGSRPGVYYAHLSDMDAMPKPELEVIAYHEG
                     LPGHHMQISIAQELTGVPRFRTQAKFTAYSEGWGLYAEWLAKEMPNTYQDPYSEFGRL
                     SSEMWRAIRLVVDTGLHSKGWTEQQAVDYFDANSAVPLAAIQSEVQRYLVMPGQATAY
                     KIGMIRIQELRRKAEAELGDRFDIKGFHDTVLDGGALPLTLLDRRVDQWIASRRNRPK
                     "
     gene            complement(4002..>4209)
                     /locus_tag="VIO83_03540"
     CDS             complement(4002..>4209)
                     /locus_tag="VIO83_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005762849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8330382.1"
                     /translation="GANARHAISTGVIGGMLFATLLGLLLIPIFFIVVRRLLGDKMDE
                     PSREYLESHKDQDGQGTPQAQGGH"
BASE COUNT          801 a         1352 c         1395 g          661 t
ORIGIN      
        1 agcagcggat cgtcgctgcc cgcgcgcagg ctgcgccaca ggtcgcgctg ggccgggtcc
       61 aggttgtcct cgtcgaaacg gtagtaggcc gacacgcctg cgcggcgatc gcgcaccagc
      121 ccggcgtcct tgagcttggc caggtgggtc gaaacgcgcg gctgcgccag gcgggtgatc
      181 gaggacagct cggccacggt cagttcctcg ctggccagca gggccagcag gcgcacgcgg
      241 gtggcgtcgg cgaacacctt cagacgggcc gaccaggctt ccagatccat gatccatctc
      301 tatatcgcga tagggcgata ttatgtccgt cgagccgctg gcggtcaatt ccatacgtag
      361 ccgcatggtg cgaggctaca atcgagggtc tcgcaccttg agcagggaaa gaccgtggat
      421 ttcggataca ccgaagagca gctgatgatc caggacgtgg cccgccggat cgcccaggaa
      481 aagatcgcgc ccagcgccga gcatttcgac aggaccggcg agttcccgct ggagaacatc
      541 cggctgctgg gcgagaacgg cctgatgggc atcgaggtgc cggccgagta cggcggcgcc
      601 ggcatggacc cggtcagcta cgtgctggcg atgatcgagg tggccgccgg cgatgccgcg
      661 cacgccacca tcatgtcggt caacaactcg ctgtactgct cgggcctgct caagttcgcc
      721 accgaggaac agaagcagct ttacgtgcgc gccatcgccg acggcagcca gatcggcgcc
      781 ttcgccctga ccgagccgca gtccggttcg gacgccacgg ccatgcgctg ccgcgcggtc
      841 aggcaggacg acggcacgtt cgtcatcaac ggcaagaaga gctggatcac ctccggtccg
      901 gtggccagat acatcgtgct gttcgcgatg accgcgccgg acaagggcgc caagggcatc
      961 accgccttca tcgtcgatac cgggcgcgaa ggcttccacc gcggcaagac cgaaccgaag
     1021 ctgggcatcc gcgcctcggc cacctgcgag atcgagttcg ccgattacct ggcccagccg
     1081 ggcgaggtgg tcggcggcga gggcgagggc ttccggatcg ccatgggcgt gctggatgcc
     1141 gggcgcatcg gcatcgcctc gcaggccatc ggcatcgccc gggcggcgta tgaaaagacc
     1201 atcgaatacg tgaaggaacg caaggccttc ggcgcgccga tcggcacctt ccagatgacc
     1261 caggccaaga tcgccgacat gaagtgcaag ctggacgcat cgctgctgct gaccctgcgc
     1321 gcggcgtggc tgaaagggca gggcaggaag ttcggcagcg aggcggccat cgccaagctc
     1381 accgcgtcgg aggcggcgat gtggatcacc caccaggcgg tgcagatcca tggcggcatg
     1441 ggctattcga aggagatgcc gctggagcgt tacttccgag atgccaagat caccgagatc
     1501 tacgaaggca ccagcgaaat ccagcgcctg gtgatcgcgc gcggcgagac cggcctgcgt
     1561 tagcgagcgc caggcaccgg cctgctcgcc tggcggcgag gttggcccgg tgcgttcaac
     1621 gcgatgccag gacgagggcg atcttggcaa ggcgactcgg acatccagcc cggtgatcgc
     1681 acgctgccag acctggcggt gcgtaggcgc ggcgacgata gggatccgca acgtggcgtt
     1741 cctgtaacgc tgcccgggtg gcagcaccga agcagtcgcc ggcactgcat ggacggccct
     1801 gcccggtgca atccgctaga gttcccgggc aggtatgtcc cttcccgtag gagaacgcga
     1861 tgcgtcaaac cctgatcgcc tgtgccctgg cgttggcctt gaccggcccc gctggcgcca
     1921 cgacgccgca ggccggtgcg gcctggaccc caccggcggc catttcccag gccgacatca
     1981 aggccgagac cgaacgtctg aaccagtggt ttgagaagaa gtacgaggag caactgcgct
     2041 tcagtccgct gcaattgacc tttcaggggc gcaaggacct gtacgaccag ctggacgacc
     2101 tgtctgagca ggccacgctc gaccaggtgg cctggcagaa gtccagcgtc gaggaaatgg
     2161 aaaagacctt cgcctacgcc aagctcagcg acgaaggcaa gttttcctac gacctgtgga
     2221 agctgcagta cgagaacgcg cgtgacggtc tcccgttcct ggtcgatcgc tacgccttcg
     2281 accagatgaa gggcgcgcag agtttccttc ccaccatcct gatcaacttc cacaaggtgg
     2341 acgaggaggc cgactacagc gcttacgtcg cgcgcctggc ggcaaccgcg cgcgcgttcg
     2401 accagttgct ggagcgcgcg cgcaagtccg ccaggcaggg cgtgcgtccg cccaaattcg
     2461 cctatgaagg ggtgatcgac caggcgcgca aggtgattgc cggcgcaccg ttctcggagg
     2521 gcgaggattc gtccatctgg gccgacgccc aggccaaggc cgatgcgctg gtgaagaacg
     2581 gcaagatcga tgccgaacgc gccgcatccc tgaagcagca ggcgcgcacg gtactactgg
     2641 aacaattcaa gccgggctac gagcggatca ttgcctggtg cgagcaagac ctgccgaacg
     2701 cggcagtcaa tgcttccggc gtaggcacta cccatgccaa cggcaaggcg tactaccagt
     2761 atcagctgcg ccagatgacc accaccaaca tgaccgccga ccagatccat gcgctgggcc
     2821 tgaaggaagt cgagcggatc aagggcgaga tgaacgcgct gaagaatcgg gtcggcttca
     2881 agggcgacct ggatgcgttc ttcgccttca tcgattccga cccgcagttc aagttcccca
     2941 acaccgatgc cggccgcgag gcctacatcg accaggccac ccgggtcatc gccaacatca
     3001 aggcgcagtt gcccaactat ttcggcctgc tgcccaaggc cgacctggtg gtcaagcgcg
     3061 tggaggcctt ccgcgagcag ccgggcgcgg cccagcacta tttcccgggt acccccgatg
     3121 gctcgcggcc tggcgtgtac tacgcccacc tgtccgacat ggatgccatg cccaagccgg
     3181 agctcgaggt catcgcctat cacgagggct tgcctggaca ccacatgcag atctcgatcg
     3241 cgcaggagct taccggggtg cccaggtttc gtacccaagc caagttcaca gcctattcgg
     3301 aaggctgggg cctgtatgca gagtggctgg ccaaggagat gccgaacacc tatcaggatc
     3361 cgtattccga gttcggccgc ctgagttcgg aaatgtggcg cgcgatccgc ctggtggtcg
     3421 ataccggcct gcactccaag ggctggaccg agcagcaggc ggtcgactac ttcgatgcca
     3481 acagcgccgt cccgctggca gcgatccagt ccgaagtgca gcgctacctg gtcatgccgg
     3541 gacaggccac cgcttacaag atcggcatga tccggatcca ggagctgcgc aggaaggccg
     3601 aggccgagct cggtgacaga ttcgacatca agggcttcca cgacacggtg ctggacggtg
     3661 gtgcgctgcc actcacactg ctggatcgcc gcgttgacca gtggatcgcc agccgcagga
     3721 atcggccgaa gtagcagcgc ctcggttgcc acctggacgt ggacgaacga ctccggccga
     3781 gctgttcgtg atgggtggcc cgtggccgag catggacgag cgagcgcgcc agcctgggcg
     3841 aatgggacag tgcaacgacc tgggttgtgc tcccccactt ccatggcggc ggcctgggtc
     3901 ctggtcagcc gctggacgat accggctgac gcagtccgag gatctcacca aaaaagaccc
     3961 cggcagcgcc ggggtctttt cgtatcgcgc gaagcgatga ctcagtggcc gccctgtgcc
     4021 tgtggcgtgc cttggccgtc ctggtccttg tggctttcca ggtattcacg cgacggttcg
     4081 tccatcttgt cgcccagcag ccggcggacc acgatgaaga agatcgggat cagcagcagg
     4141 cccaggaggg tggcgaacaa catgccgccg atcacgccgg tggagatggc gtggcgcgcg
     4201 ttggcgccg
//
LOCUS       DATCUN010000091         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_229039, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000091 DATCUN010000000
VERSION     DATCUN010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229039"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..608
                     /locus_tag="VIO83_03545"
     CDS             273..608
                     /locus_tag="VIO83_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330383.1"
                     /translation="MPRHLCLTRHSPGIVTRIECMLHPLAGGQGLWMLFCAAGLNAAQ
                     PSAVKAQGPFHGASEAQGVLDEIVENLLRQGYLAVDEPAIWRLHLQAELRRQDAGRGH
                     PPGHFRLQP"
     gene            794..1282
                     /locus_tag="VIO83_03550"
     CDS             794..1282
                     /locus_tag="VIO83_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HEY8330384.1"
                     /translation="MERFIENTMYAARWLLAPIYLGLSLALLALALKFFQEIFHVLPH
                     VFELAEAELILVLLSMIDMALVGGLLVMVMISGYENFVSQLDIDEGKEKLEWLGKMDS
                     GSLKLKVAASIVAISSIHLLRMFMDAQKIPNDKLLWYVVIHMTFVVSAFAMAYVDKLT
                     KH"
     gene            1498..1803
                     /locus_tag="VIO83_03555"
     CDS             1498..1803
                     /locus_tag="VIO83_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6482 family protein"
                     /protein_id="HEY8330385.1"
                     /translation="MNLHDLSAHALAGRIDGLDLISLEGGIYVLEAQMDGRPHSLVDE
                     HGRVCHLRSVEHARDLLQEVPLLPFHLVHHSPYDEMCGLAEGSREPLRVPIGMRSHW"
     gene            1800..>2785
                     /locus_tag="VIO83_03560"
     CDS             1800..>2785
                     /locus_tag="VIO83_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEY8330386.1"
                     /translation="MTDAELPAALQRPLALLDVQATGLHPQRDGIWEIAVLLVDEGRV
                     RRRLSWLLDPGQPLPASVVALSGVLAAELHGQPRFAEIAGALRQVLHGRLLVGHNLRF
                     DLAFLRQALLAAGLPLRARQLCTLRLARQLHPELPAYGLDALCAHFAIPRFLRHRASV
                     DVEACWQLLQCLARQDAAAFERSLRGQLRKAAVPHQLSAERLAALPERPGVYYFHGEG
                     GALLYVGKSRNLRRRVQSHFQNDHASRRSLQIAQQVREIRVQPTAGELGALLLEAAEV
                     ARLQPLYNRQLRRQRELLSWTLRGTADGLQPELQALQQLQPGIEHAGLFRARR"
BASE COUNT          398 a          955 c          925 g          507 t
ORIGIN      
        1 catggcaagg ccgccgccgc cgcgcctgtg ccgggcaagc cgaccagcca ggcccgccgc
       61 ctgcgccgct gaggcgctgc agcggattgg gcggcgaccg ccgacctggt cattttatga
      121 ccaggtcggc gtgcggagca cccggcggcc ctccgcatca tctgcccccg cgtcatgcac
      181 aaggttatcc acagatcgcg gggataactg gggctttcct cgcaggcaca gtgcgactag
      241 gctcaatagt gtccattggg aggaaagtcg ccatgccgcg ccacctctgt ctgacccggc
      301 acagtccggg aatcgtcacc cgtatcgaat gcatgctcca tccgctggct ggcgggcagg
      361 gactgtggat gctgttctgc gcggccggcc tgaatgccgc ccagccttcg gcggtcaagg
      421 cgcaaggccc gttccacggg gcttctgagg cgcagggggt gctcgacgag atcgtcgaaa
      481 acctgttgcg tcagggttac ttggcggtcg acgaaccggc tatctggcgt ctgcatctgc
      541 aggccgaact gcgccggcag gatgccggac gcggccatcc cccggggcat ttccgcctgc
      601 agccctaggc cgccgcgctg cggctgttgg ggctgccccg tgctcggccc ggcaggcgcc
      661 gcgccgcccg cagccattca tccagaactc atccacacag ttatccacag gctggcccgg
      721 caagtccggc tgcagtcgcg ccggcaggcc cggtatactc ggcctccctt gtgcaatgcc
      781 gtgcctctat cccatggaac gattcatcga aaacaccatg tatgccgcgc gctggctgct
      841 ggcgccgatc tatctcgggc tgtcgctggc gctgttggcg ctggccctga agtttttcca
      901 ggaaatcttc cacgtcctgc cccatgtgtt cgaactggcc gaggccgagc tgatcctggt
      961 gctgctgtcg atgatcgaca tggcgctggt cggtggcctg ctggtgatgg tgatgatttc
     1021 cggctacgag aacttcgtct cccagctcga catcgacgag ggcaaggaaa agctcgagtg
     1081 gctgggcaag atggattccg gctcgctgaa gctgaaggtg gcggcatcga tcgtggcgat
     1141 ctcctcgatc cacctgctgc gcatgttcat ggacgcgcag aagatcccca acgacaagct
     1201 gttgtggtac gtggtcatcc acatgacctt cgtcgtctcg gcctttgcca tggcctacgt
     1261 ggacaagctg accaagcact gaaagcctgc cacggactgc ggcgcgtcgg cagcttcctg
     1321 ctggccgtgg cccgcgaggc cgtgaaccgg ctttaggcta tccatgactg cctcctgcac
     1381 cgcccgtctt gcctctggca gacgggcggt ggcgtttctg ccttgcgcag agttgatctt
     1441 gtacaaatac tgtacttgtt gcattgtttg tatagattca ctgccgaggt gattgccatg
     1501 aacctgcacg acctgtccgc ccacgccctt gccgggcgca tcgacggcct cgacctgatc
     1561 tccctggagg gcggcatcta cgtcctcgag gcgcagatgg acggccgccc gcacagcctg
     1621 gtcgacgagc acgggcgcgt ctgccacctg cgctcggtgg agcacgcccg cgatctgctg
     1681 caggaggtgc cgctgctgcc gttccaccta gtccaccact cgccctacga cgagatgtgc
     1741 ggcctggccg agggcagtcg cgagccgctg cgcgtgccga tcggcatgcg ttcgcactgg
     1801 tgaccgacgc tgagctgccg gcggcgctgc agcgtccgct ggcgctgctg gatgtgcaag
     1861 ccaccggcct gcatccgcag cgcgacggca tctgggaaat cgccgtgctg ctggtcgacg
     1921 agggccgggt gcgccgtcgg ctgagctggc tgctcgaccc cgggcagccg ctgccggcca
     1981 gcgtggttgc cctgagcggt gtgctggctg cggagctgca tggccagccg cgcttcgccg
     2041 agattgccgg cgcgctgcgg caggtgttgc acgggcgtct gctggtcggc cacaacctgc
     2101 gtttcgatct ggccttcctg cgccaggcac tgctcgccgc cgggttgccg ttgcgtgccc
     2161 gccagctgtg taccctgcgc ctggcccgcc agctgcatcc cgagctgccg gcctacggcc
     2221 tcgatgccct gtgcgcgcac tttgctatcc cgcgcttcct gcgccaccgt gcgtcggtcg
     2281 atgtcgaggc ctgctggcag ctgctgcagt gcctggcccg gcaggatgcc gcggcgttcg
     2341 agcgcagcct gcgcgggcaa ttgcgcaagg ctgcggtgcc gcatcagttg agtgccgagc
     2401 ggctggccgc gctgcccgag cgcccaggcg tctactactt ccatggcgag ggcggggcgc
     2461 tactctatgt cggcaagagc cgcaacctgc gtcgccgcgt gcagtcgcac ttccagaacg
     2521 accacgccag ccggcgcagc ctgcagatcg cccagcaggt gcgcgagatc cgcgtacagc
     2581 cgaccgccgg cgagctgggt gcactgctgc tggaggccgc cgaggtggcg cgcctgcaac
     2641 cgctgtacaa ccgccagctg cgccgccagc gcgagttgct gtcgtggacc ctgcgcggca
     2701 cggcggacgg cctgcagccc gagctgcagg cgctgcagca gctgcagccg ggcatcgagc
     2761 acgccggact gtttcgcgct cgccg
//
LOCUS       DATCUN010000092         3031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_97984, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000092 DATCUN010000000
VERSION     DATCUN010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97984"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2425
                     /gene="aceE"
                     /locus_tag="VIO83_03565"
     CDS             <1..2425
                     /gene="aceE"
                     /locus_tag="VIO83_03565"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY8330387.1"
                     /translation="IRSLIRWNALATVMRANKKDPDLGGHISTFASSATLYDIGFNYF
                     FKAPTEEHGGDLIYFQGHASPGVYARAFLEGRISEDQLNNFRQEVDGNGLSSYPHPWL
                     MPDFWQFPTVSMGLGPIQAIYQARFMKYLEHRGFIPAGKQKVWCFLGDGETDEPESLG
                     AIALAGREKLDNLIFVVNCNLQRLDGPVRGNGKIIQELEGVFRGADWNVVKVIWGRMW
                     DPLFAKDTKGLLQKRMDEAVDGEYQNYKANDGKFVREHFFGARPELLEMVKDLSDEEI
                     WKLNRGGHDPFKVYAAYHEAVNHTGQPTVVLAKTIKGYGTGSGQAQNITHNVKKVDLE
                     SLKAFRDRFGIPIADDQLEELPFYKPEPDSPEMKYLLKSREKLGGFVPQRRAKSFSIP
                     TPPLETLKAILDGTGDREISTTMAFVRILSQLVKDKDLGSRIVPIIPDEARTFGMEGM
                     FRQLGIYSSVGQLYQPVDKDQVMFYREDKKGQILEEGINEAGAMSSWIAAATAYSNHN
                     QPMLPFYVFYSMFGFQRIGDLAWAAGDSRARGFLIGGTAGRTTLNGEGLQHEDGHSHI
                     LASTIPNCRTYDPTYAYEMAVIIREGIRQMTEEQQNVFFYLTAMNEAYAQPAMPEGVE
                     DGIIKGMYLLEEDNRDAACHVQLLGSGAILREVREAAKILREEYNVGADVWSVTSFNE
                     LRREGLAVERRNRLHPEQKPELSYVEQCLSERKGPVVASTDYMKLFADQIRQWVPVKE
                     YKVLGTDGFGRSDSRKKLRHFFEVDRNWVVLTALEALADRGEIERTVVAEAIAKFGID
                     TNKPNPLDC"
     gene            2437..>3031
                     /locus_tag="VIO83_03570"
     CDS             2437..>3031
                     /locus_tag="VIO83_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003209438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY8330388.1"
                     /translation="MSELIRVPDIGSGDGEVIELLVKVGDRIEADQSLLTLESDKASM
                     EIPAPKAGVIKSLKIKLGDKLKTGDELLELEAEGAAASAPAAEAPAAAPAAPVGESLQ
                     PVHVPDIGSAGKARIIELLVKAGDVVSAEQSLLVLESDKASMEIPCPQAGTVDSVAVK
                     LNDEVGTGELILTLRVAGAVPAAAPVAAAPAAAPAAPA"
BASE COUNT          588 a         1046 c          963 g          434 t
ORIGIN      
        1 catccgctcg ctgatccgct ggaacgccct ggccacggtg atgcgcgcga acaagaagga
       61 cccggacctg ggtggccaca tctccacctt cgcctccagt gcgaccctgt acgacatcgg
      121 cttcaactat ttcttcaagg cgccgaccga agaacacggc ggcgacctga tctacttcca
      181 gggccacgcc tcccccggcg tctacgcccg cgccttcctc gaaggccgca tcagcgaaga
      241 ccagctgaac aacttccgcc aggaggtgga cggcaacggc ctgtcctcct acccgcaccc
      301 gtggctgatg ccggacttct ggcagttccc caccgtgtcg atggggctgg gcccgatcca
      361 ggcgatctac caggcgcgct tcatgaagta cctggagcac cgcggcttca tccccgccgg
      421 caagcagaag gtctggtgct tcctcggcga cggcgagacc gacgagccgg aatccctcgg
      481 cgccatcgcc ctggccggcc gcgagaagct cgacaacctg attttcgtgg tcaactgcaa
      541 cctgcagcgt ctcgacggcc cggtgcgcgg caacggcaag atcatccagg aactcgaagg
      601 cgtgttccgc ggtgccgact ggaacgtggt caaggtgatc tggggccgca tgtgggaccc
      661 gctgttcgcc aaggacacca agggcctgct gcagaagcgc atggacgagg ccgtcgacgg
      721 cgagtaccag aactacaagg ccaacgacgg caagttcgtc cgcgagcact tcttcggtgc
      781 ccgtccggag ctgctggaga tggtcaagga tctctccgac gaggagatct ggaagctcaa
      841 ccgtggcggc catgacccgt tcaaggtcta cgcggcctac cacgaggcgg tcaaccacac
      901 cggccagccg accgtggtac tggccaagac catcaagggc tatggcaccg gctccggcca
      961 ggcgcagaac atcacccaca acgtcaagaa ggtcgacctg gaaagcctca aggccttccg
     1021 cgaccgcttc ggcatcccga ttgcggacga ccagctggaa gagctgccgt tctacaagcc
     1081 ggaaccggac agcccggaaa tgaagtacct gctgaagagc cgcgagaagc tcggcggttt
     1141 cgtgccgcag cgccgcgcca agagcttcag catcccgacc ccgccgctgg agaccctcaa
     1201 ggcgatcctc gacggcaccg gcgaccgcga aatctccacc accatggcct tcgtgcgcat
     1261 cctctcgcag ctggtcaagg acaaggacct gggttcgcgc atcgtgccga tcatcccgga
     1321 cgaggcgcgc accttcggca tggaaggcat gttccgccag ctcggcatct actcctcggt
     1381 cggccagctg taccagccgg tggacaagga ccaggtgatg ttctaccgcg aggacaagaa
     1441 gggccagatc ctcgaggaag gcatcaacga ggccggcgcc atgtccagct ggattgccgc
     1501 ggccaccgcc tacagcaacc acaaccagcc gatgctgccg ttctacgtgt tctactcgat
     1561 gttcggcttc cagcgcatcg gcgacctggc ctgggcggcc ggcgacagcc gtgcgcgcgg
     1621 cttcctgatc ggcggcaccg ccgggcgcac caccctcaac ggtgaaggcc tgcagcacga
     1681 ggacggccac agccacatcc tggcctcgac catccccaac tgccgcacct acgacccgac
     1741 ctatgcctac gagatggcgg tgatcatccg cgaaggcatc cgtcagatga ccgaagagca
     1801 gcagaacgtg ttcttctacc tgaccgcgat gaacgaggcc tacgcccagc cggccatgcc
     1861 ggaaggcgtc gaggacggca tcatcaaggg catgtacctg ctcgaagagg acaaccgcga
     1921 cgccgcctgc cacgtgcagc tgctcggctc cggcgccatc ctgcgcgaag tgcgcgaggc
     1981 ggcgaagatc ctccgcgagg agtacaacgt cggcgccgac gtgtggagcg tcaccagctt
     2041 caacgagctg cgccgcgaag gcctggccgt ggagcgccgc aaccgcctgc acccggaaca
     2101 gaagcccgag ctgagctacg tcgagcagtg cctgtccgag cgcaagggcc cggtggtcgc
     2161 ctccaccgac tacatgaagc tgttcgccga ccagatccgc cagtgggtcc cggtcaagga
     2221 atacaaggtg ctcggcaccg acggcttcgg ccgcagcgac agccgcaaga agctgcgcca
     2281 cttcttcgag gtggaccgca actgggtggt gctgaccgcg ctggaggccc tggccgaccg
     2341 cggcgagatc gagcgcaccg tggtggccga ggcgatcgcc aagttcggca tcgacaccaa
     2401 caaacccaac ccgctggact gctgaggaga gaagcgatga gtgaactgat tcgcgtaccc
     2461 gatatcggca gcggcgatgg tgaagtgatc gaactgctgg tcaaggtcgg cgaccgcatc
     2521 gaagccgacc agagcctgct gaccctggag tccgacaagg ccagcatgga gatccccgcg
     2581 cccaaggccg gggtgatcaa gagcctgaag atcaagctgg gcgacaagct gaagaccggc
     2641 gacgaactgc tcgagctgga agccgagggc gccgccgcca gtgcgccggc ggccgaagct
     2701 ccagccgcgg ctccggccgc cccggtcggc gagagcctgc agccggtgca cgtgccggac
     2761 atcggctcgg ccggcaaggc ccgcatcatc gagctgctgg tcaaggccgg cgacgtggtc
     2821 agcgccgagc agtcgctgct ggtgctggag tcggacaagg cgagcatgga aatcccctgc
     2881 ccgcaggccg gcaccgtcga cagcgtggcg gtcaagctca acgacgaggt tggcaccggc
     2941 gagctgatcc tcaccctgcg cgtggccggc gccgtgccgg ccgccgctcc cgttgccgcg
     3001 gctcctgctg ctgcccctgc ggcgccggct c
//
LOCUS       DATCUN010000093         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_229338, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000093 DATCUN010000000
VERSION     DATCUN010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229338"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VIO83_03575"
     CDS             complement(<1..227)
                     /locus_tag="VIO83_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330389.1"
                     /translation="MKGILGIAALALAALTALPASAGTLYKCVGADNIPSYVSKRVAG
                     ASCSVVSSYRPDRSAPRRYVPPAPTPVAAAVV"
     gene            556..>1668
                     /gene="miaB"
                     /locus_tag="VIO83_03580"
     CDS             556..>1668
                     /gene="miaB"
                     /locus_tag="VIO83_03580"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY8330390.1"
                     /translation="MRGKLYIKTHGCQMNEYDSSKMADVLAAAEGLELTDDPAEADVL
                     LMNTCSIREKAQEKVFSQLGYWKALKNNGRGVIIGVGGCVASQEGEAIIKRAPHVDLV
                     FGPQTLHRLPELIRQRRESGKSQVDISFPEIEKFDRLPEPRADGGSAFVSIMEGCSKY
                     CSFCVVPYTRGTEVSRPFEDVVVEVAQLAAQGVREINLLGQNVNAYRGPYGEGEFADL
                     GLLIRTIAEIDGVGRIRFTTSHPLEFSDSLVDAYRDVPQLANFLHLPVQAGSDRVLSA
                     MKRGYTALEFKAKIRKLRAVRPDISISSDFIVGFPGETDEDFEKTMKLIEDIGFDHSF
                     SFIYSRRPGTPAADLEDTISDAEKHARLSRLQARINE"
BASE COUNT          286 a          590 c          543 g          249 t
ORIGIN      
        1 acgacagcgg cggcgaccgg ggtcggggcc ggcggcacat accggcgtgg ggcgcttcgg
       61 tcgggccggt aactgctgac cacactgcag gacgcgcccg ccacccgctt gctcacgtag
      121 ctggggatgt tgtcggctcc gacgcacttg tacagcgtgc ccgcactggc cggcaaagcg
      181 gtcagcgcag ccaaggccag cgcagcgatc cccaagatcc ccttcatggc ggcgagtgtc
      241 ccaagttaac ggagccttgc caagtctttg acgcgacact gacggacttc aggccccaac
      301 ccccgacggc cgtcacaccg cgggccggcc cgggcgcccg gcgccctccc cccttccccg
      361 ctacaatggg cggcttccca gcagccttcc ggctgcctgc ccgcccaccc gactggaata
      421 agcgccatga ccgggacgcc agcttcgacc gcgccaactt cggccggctc cgccttgcct
      481 gcctccgctt cgcccgattc gccccgcccc gacctgatcc ccctgcccgg cacccggccg
      541 aaggtggacg gaccggtgcg cggcaagctc tacatcaaga cccacgggtg ccagatgaac
      601 gagtacgact cgtccaagat ggccgacgtg ctcgccgccg ccgaggggct ggagctcacc
      661 gacgacccgg ccgaggccga cgtgctgttg atgaacacct gctcgatccg cgaaaaggcg
      721 caggagaagg tattcagcca gctggggtac tggaaggcgc tgaagaacaa cggccgtggt
      781 gtgatcatcg gcgtgggcgg ctgcgtggcc tcgcaggaag gcgaagcgat catcaagcgc
      841 gccccgcacg tggacctggt gttcggcccg cagaccctgc atcgcctgcc cgagctgatc
      901 cggcagcggc gcgagtcggg caagtcgcag gtggacatca gcttcccgga gatcgagaag
      961 ttcgaccgcc tgcccgagcc gcgcgccgac ggtggctcgg cgttcgtgtc gatcatggaa
     1021 ggctgctcca agtactgctc gttctgcgtg gtgccctaca cccgtggcac cgaagtgagc
     1081 cgtccgttcg aggacgtggt ggtggaagtg gcccagctgg ccgcccaggg cgtgcgcgag
     1141 atcaacctgc tgggccagaa cgtcaacgcc taccgcggcc cgtatgggga gggcgagttc
     1201 gccgatctcg gcctgctgat ccgcaccatc gccgagatcg acggggtggg ccggatccgc
     1261 ttcaccacct cgcacccgct ggagttcagc gactcgctgg tggatgccta ccgcgacgtg
     1321 ccgcagctgg ccaacttcct tcacctgccg gtacaggcgg gcagcgaccg ggtgctgtcg
     1381 gcgatgaagc gcggctacac cgcgctggaa ttcaaggcca agatccgcaa gctgcgggcg
     1441 gtgcgcccgg acatctcgat cagctcggac ttcatcgtcg gcttccccgg cgagaccgac
     1501 gaggatttcg agaagaccat gaagctgatc gaagacatcg gcttcgacca cagcttctcc
     1561 ttcatctatt cgcgccgccc gggcacgccc gcggccgatc tggaagacac catcagcgac
     1621 gccgagaagc acgcccgact gtcgcggctg caggcgcgga tcaacgaa
//
LOCUS       DATCUN010000094         4188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_210606, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000094 DATCUN010000000
VERSION     DATCUN010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210606"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VIO83_03585"
     CDS             <1..294
                     /locus_tag="VIO83_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HEY8330391.1"
                     /translation="TRSAPRYKLYALAGGPPLRPGMLRVAEGGAAIAVEVWELPSAEL
                     GSFLTGIPAPLGLGKVELADSRWETGFICDAYGLEGAEDITACGGWRAWLQSR"
     gene            527..973
                     /gene="azu"
                     /locus_tag="VIO83_03590"
     CDS             527..973
                     /gene="azu"
                     /locus_tag="VIO83_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEY8330392.1"
                     /translation="MFRKLAAISLLGLFSAPLWAAECSVDIQAADQLQFDTSAISVDK
                     SCKTFTVNLTHTGQMAKNVMGHNWVLSTNADMQGVLTDGTGAGLDNNYLKPDDTRVIA
                     HTKVIGGGEKDAVTFEVAKLKAGEQYMFYCSFPGHAALMKGTLELK"
     gene            1263..1733
                     /locus_tag="VIO83_03595"
     CDS             1263..1733
                     /locus_tag="VIO83_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8330393.1"
                     /translation="MITLNLNGKDHPLDVAEDMPLLWAIRDVVGYTGTKFGCGMGQCG
                     ACTVHLDGQAVRGCLTPVGQAVGKRVTTIEALHEDPVGKVVQQAWLEHAVAQCGFCQG
                     GQIMAATALLRDNRSPDEGQIKAAMVGNICRCGTYNRIRTAIDSAAGKLREGQA"
     gene            1730..4060
                     /locus_tag="VIO83_03600"
     CDS             1730..4060
                     /locus_tag="VIO83_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY8330394.1"
                     /translation="MSIQEREDLTQDDVVVANLSRRGFLKGLAATGALVIAANWGMPS
                     AFAAAKKNVFGADAMPHGWVDSPKVFLSIAADGLVTFVCSRSEMGQGVRTSLAMVVAD
                     ELEAEWEMMRVVQAPGDETVYGNQDTDGSRSMRHWFEPLRRCGAAARQMLEQAAAQQW
                     QVPVAECKAQGHKVLHAASGRSLGYGELAEAAAALAVPARKSLRLKKPEEFRYIGKEA
                     TRAIDGADIVHGRAQFGADVRFDNMLYAVIARPPVYGGTLKSHDASTALQVPGVVKVV
                     EIAATPLPSEFQPLGGVAVVAGNTWAAIKGREALKLEWDDGANAAYDSPSYRQALSKA
                     ARAPGKRVRNVGNVDAAFKKAKQVLEAEYYLPHLAQAAMEPPVATAIFADGACEVWAP
                     SQAPQVTRERIAKRLELPVDKVKVNVTLLGGGFGRKSKPDFIIEAALLAKEFPGRHVR
                     VQWTREDDLQHSYFHTVSLEYLRGALDAKGQPSAWLHRSAAPSIGALFGPDPKHQGPF
                     ELGMGFINLPYRVPNLRLENPQAAAHTRVGWYRSVSNIPHAFAVQSFIAELAAAAGKD
                     HRDYLLELLGPARKIDPSSLGDSWNYGESPKQYPLDTGRLRAVVEEATRQAGWGQELP
                     KGRGLGLAVHYSFVTYVAVVLEVEVRTDGALLVHKATMAVDCGPQINPERIRSQMEGA
                     CVMGLSNAALSEISFRAGQVEQSNLHNYQVARLPLAPREIAVHLVTPKGEVPLGGVGE
                     PGVPPIAPALCNAIFAATGKRIRELPIRNQLMDWQV"
     gene            4060..>4188
                     /locus_tag="VIO83_03605"
     CDS             4060..>4188
                     /locus_tag="VIO83_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010208365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY8330395.1"
                     /translation="MDSVDLNVLRSVLAWRQAGQRVRLYTVVQTWGSAPRPPGAMLA"
BASE COUNT          714 a         1491 c         1406 g          577 t
ORIGIN      
        1 acccgcagcg cgccgcgtta caagctgtac gcgctggccg gtggcccgcc gctgcgcccg
       61 ggcatgctcc gtgtcgccga gggcggcgcg gcgattgccg tggaagtctg ggaactgccg
      121 agcgccgagc tgggctcctt cctcaccggc atcccggcgc cgctgggcct gggcaaggtg
      181 gagctggccg acagccgctg ggaaaccggc ttcatctgcg acgcctacgg gctggagggc
      241 gccgaggaca tcaccgcctg cggcggctgg cgcgcctggc tgcagtcgcg ctgagtcgcg
      301 tcacccggcc ctcgcggcag ccggcacaac agcacggctg ccgcccgcag actgaccggc
      361 aaccggcgca aacccatcgc cttgactatc ctcaagcacc gagcgatccc ccgcttgcct
      421 gccgtgccga ccacgtcggc gcggtcctgc accgatgccg ggactccgcc cttcgctggg
      481 gccatcccgt tcggagcgtt cagcccaacc tacccaggag gctcgtatgt ttcgcaaact
      541 cgctgccatc tccctgctcg gtctgttcag cgcccccctc tgggcggccg agtgttcagt
      601 ggacatccag gctgccgacc aactgcagtt cgacaccagc gccatcagcg tggacaagag
      661 ctgcaagacc ttcaccgtca acctcaccca caccggccag atggcgaaga acgtgatggg
      721 ccacaactgg gtgctgagca ccaacgccga catgcagggc gtgctgaccg acggcaccgg
      781 cgccggcctc gacaacaact acctgaagcc cgacgacacg cgggtcatcg cccacaccaa
      841 ggtgatcggt ggcggcgaga aggatgccgt gaccttcgag gtagccaagc tcaaggcggg
      901 cgagcagtac atgttctact gcagcttccc cggccatgcc gcgctcatga agggcacgct
      961 cgaactgaag tgaacctccc gacagcaaac gctccagcct gagcggagcg atctgtcgcc
     1021 aaagccccgg ccctgtggtc ggggcttttt ttccagcccg cactggattg gcgcaaaaat
     1081 acgcctgacg cacaggatgt gatcccggca gcaatcgatc cgatggtata aacggtgtct
     1141 tcgcttgttg agctaaattc aagcaaacct ctcggatctg cagccggccg ctctgccagc
     1201 aggtgctggt cgggccgcac acgccggtgc tgtgcaatga cccaatgaat ggatacgcct
     1261 cgatgattac cctgaacctg aatggcaagg atcacccgct cgatgtagcg gaggacatgc
     1321 ccctgctgtg ggccatacgc gatgtcgtcg gttacaccgg caccaagttc ggttgtggca
     1381 tgggacagtg cggcgcctgc accgtgcacc tcgacggcca ggccgtgcgt ggctgtctca
     1441 ccccggtcgg ccaggcggtc ggcaaacggg taaccaccat cgaggcgctg cacgaagacc
     1501 cggtcggcaa ggtggtgcag caggcctggc tggagcatgc cgtcgcccaa tgcggcttct
     1561 gccagggcgg gcagatcatg gcggccaccg cgctgctgcg ggacaaccgc agtcccgacg
     1621 aggggcagat caaggcggcg atggtcggca acatctgccg ctgcgggacc tacaaccgga
     1681 ttcgcacggc catcgacagc gccgccggca agctgcggga gggccaggca tgagcatcca
     1741 ggaacgggaa gacctcactc aggatgacgt cgtcgtcgcc aacctcagcc gccgcggctt
     1801 cctcaagggc ctggccgcca ccggcgcgct ggtgatcgcc gccaactggg gcatgcccag
     1861 cgccttcgcc gcggcgaaga agaacgtgtt cggcgccgac gccatgcccc acggctgggt
     1921 cgacagcccc aaggtatttc tcagcatcgc ggccgacggc ctggtgacct tcgtctgcag
     1981 ccgctcggag atgggccagg gcgtgcgcac cagcctggcc atggtggtgg ccgacgagct
     2041 ggaagccgag tgggagatga tgcgcgtggt gcaggcgccg ggcgacgaga cggtctacgg
     2101 caaccaggac accgacggct cgcgcagcat gcgccactgg ttcgagccgc tgcgccgctg
     2161 tggcgccgcc gcgcggcaga tgctcgagca ggccgccgcc cagcaatggc aggtgccggt
     2221 ggccgagtgc aaggcgcagg gccacaaggt gctgcacgct gccagcggac gcagcctggg
     2281 ctacggcgaa ctggccgagg ccgccgccgc gctggcggtg ccggcgcgca agagcctgcg
     2341 cctgaagaaa cccgaggagt tccgctacat cggcaaggaa gcgacccgcg ccatcgacgg
     2401 cgccgacatc gtccacggtc gcgcccagtt cggcgccgat gtgcgcttcg acaacatgct
     2461 gtacgccgtc atcgcccgcc cgccggtcta cggcggtacc ctgaagagcc acgacgccag
     2521 caccgccctg caggtgccgg gggtggtgaa ggtggtggaa atcgccgcca cgcccctccc
     2581 ctccgagttc cagccgctgg gcggcgtcgc cgtggtggcc ggcaacacct gggcagcaat
     2641 caagggccgc gaggcgctca agctggagtg ggacgacggc gccaacgccg cctacgactc
     2701 ccccagctac cgccaggcgc tgagcaaggc cgcccgcgca ccgggcaagc gcgtgcgcaa
     2761 cgtcggcaac gtcgacgccg cgttcaagaa ggccaagcag gtgctggagg ccgagtacta
     2821 cctgccgcac ctggcccagg ccgccatgga gccgccggtg gccaccgcga tcttcgccga
     2881 cggcgcctgc gaagtgtggg cgcccagtca ggcgccgcag gtcacccgcg agcgcatcgc
     2941 caagcgcctg gagctgccgg tcgacaaagt gaaggtcaac gtcaccctgc tcggcggtgg
     3001 cttcggccgc aagtccaagc ccgacttcat catcgaggcc gcgctgctgg ccaaggagtt
     3061 ccccggccgg cacgtacgcg tgcagtggac ccgcgaggac gacctgcagc actcctactt
     3121 ccacaccgta tcgctggaat acctgcgcgg cgccctggac gccaagggcc agcccagcgc
     3181 ctggctgcac cgcagcgcgg cgcccagcat cggcgcgctg ttcggcccgg accccaagca
     3241 ccaggggccc ttcgagctgg gcatgggctt catcaacctg ccctaccggg tgcctaacct
     3301 gcgcctggaa aacccccagg ccgccgccca cacccgggtc ggctggtacc gctcggtgag
     3361 caacatcccc cacgccttcg ccgtgcagag cttcatcgcc gagctggcgg cggcggccgg
     3421 caaggaccac cgcgactacc tgctggagct gctcggcccg gcccgcaaga tcgacccgag
     3481 cagcctgggc gacagctgga actacggcga atcgcccaag cagtatccgc tcgacaccgg
     3541 ccgcctgcgc gcggtggtcg aggaagcgac ccgacaggcc ggctggggcc aggagctgcc
     3601 caagggccgc ggcctgggct tggcggtgca ctacagcttc gtcacctacg tggcggtggt
     3661 gctggaagtc gaagtgcgca ccgacggcgc gctgctggtg cacaaggcga ccatggcggt
     3721 ggactgcggc ccgcagatca accccgagcg catccgttcg cagatggaag gcgcctgcgt
     3781 gatgggcctg agcaacgccg cgctcagtga gatcagcttc cgcgccggcc aggtggagca
     3841 gagcaacctg cacaactacc aggtggcgcg cctgccgctg gcgccgcggg agatcgccgt
     3901 gcacctggtc acgcccaagg gcgaggtgcc tctgggcggc gtcggcgaac cgggcgtgcc
     3961 gccgatcgcc ccggcgctgt gcaacgccat cttcgccgcc accggcaagc gcatccgcga
     4021 gctgccgatc cgcaaccagc tgatggactg gcaggtctga tggacagcgt cgacctcaac
     4081 gtcctgcgca gcgtgctggc ctggcgccag gccggccagc gtgtgcggct gtacaccgtg
     4141 gtgcagacct ggggcagcgc gccgcgcccg ccgggcgcca tgctcgcc
//
LOCUS       DATCUN010000095         6091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_41972, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000095 DATCUN010000000
VERSION     DATCUN010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41972"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..623
                     /locus_tag="VIO83_03610"
     CDS             51..623
                     /locus_tag="VIO83_03610"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEY8330396.1"
                     /translation="MEQLKQKIREHGIVLSEQVLKVDAFLNHQVDPLLMQQIGAELAR
                     RFRDQGISKVLTIEASGIAPALMTALALGVPMLFARKQRSLTLTDQLLTARVYSFTKQ
                     VESTIAIAAQHLAAHDRVLIVDDFLAHGQAALGLVSIAQQAGASIAGIGIVIEKSFQP
                     GRAELERLGYRVESLARVASLSGGQVHFVE"
     gene            complement(647..1123)
                     /locus_tag="VIO83_03615"
     CDS             complement(647..1123)
                     /locus_tag="VIO83_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330397.1"
                     /translation="MYLDAQSSLTQYKVNLQFYFRLCDLLRENGLRWSEAGNRAVDAC
                     AAELESASTRMLDARDWNSLGFVASDLGWKALQLQCGAVQQLAETTLNNQSAFSAAVQ
                     EAVSSWQKDSAAALKESTGAMPISTTLQDYLQDYLHLLTPQAPGSASRKSSTRRAR"
     gene            complement(1168..2946)
                     /locus_tag="VIO83_03620"
     CDS             complement(1168..2946)
                     /locus_tag="VIO83_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8330398.1"
                     /translation="MNISALQPMTPPAVSVPRVAGNPLDLKVHAAIARANSSVSPISM
                     LLALMDWSGHLAGSPGKQLELLNLGLEQTRRLLDYVREVASASPDCPAHECVEPPRRD
                     RRFAAKEWHQWPFSLMHQSFLLSQEWWQVATHGVRGVSRHHEEVVAFAARQLLDLFSP
                     GNFLASNPLVLQRTAATGGANLVRGMIHALEDAERLAAGHPPAGTENFVVGRDVAATP
                     GKVVLKNRLIELIQYTPTTATVHPEPILIVPAWIMKYYILDLSPHNSLIKYLVDQGHT
                     VFCLSWKNPGAEDGDLGMDEYLQLGFFAALDAINAIVPDRKVHATGYCLGGTLLAIAA
                     AAMARDNDERLASISLLTAQTDFTEPGELALFIDESEVSLLEAQMHETGYLTAGQMVG
                     AFQLLRSSDLLWSRMVGEYLMGERAEMIDLMAWNADATRMPARMHSQYLRRLFLNDDL
                     SEGRYPVGGRPVSLSDIDTPVFCVATTQDHVAPWRSVFKLHYLSPTEITFVLTSGGHN
                     AGIVSEPGRPRRHYQVLQRPVGGNYVAPDAWLASAPRHEDSWWLEWLAWLQARSSTPV
                     AAPATGAPEQGYPVVADAPGEYVRQK"
     gene            complement(3162..3581)
                     /locus_tag="VIO83_03625"
     CDS             complement(3162..3581)
                     /locus_tag="VIO83_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012274807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8330399.1"
                     /translation="MNLIKKFLAAQAAVLALWAVGAQADSDDNIAERLKPVGEVCIAG
                     QECKGVGAVAAAAGGARSADDIIAKNCGACHSTGVLNAPKIGDAADWKARAAERGGVD
                     ALLKSAIGGRNAMPPKGTCADCSDDELLGALKKMSGL"
     gene            complement(3727..4275)
                     /locus_tag="VIO83_03630"
     CDS             complement(3727..4275)
                     /locus_tag="VIO83_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8330400.1"
                     /translation="MDVGARLYAIRKLKGLSQRELAKRAGVTNSTISMIEKNSVSPSI
                     SSLKKVLGGIPMSLVEFFSMDVAQDDQAQVVYKSDELIDIGSGAVAMKLVGRAFPNRT
                     LAFLDETYPPGADTGREMLSHEGEEAGMLVDGRLELTVGEEVFILDTGDSYYFRSDQP
                     HRFRNPFEQPARLISATTPAAF"
     gene            complement(4405..4758)
                     /locus_tag="VIO83_03635"
     CDS             complement(4405..4758)
                     /locus_tag="VIO83_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8330401.1"
                     /translation="MAIRRLHSEPRYSEIVIHNGTVHLGGQLADDLSGDIRQQTRETL
                     ASIDRLLAEAGTDKTRILSTTIYLRDIATDFAGMNEVWDAWVAPGASPARATVEARLY
                     APEVRVEMMVIAALP"
     gene            4899..5387
                     /locus_tag="VIO83_03640"
     CDS             4899..5387
                     /locus_tag="VIO83_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY8330402.1"
                     /translation="MSSPHKSRRDLDRIDRNILRILQEEGRISFTELGERVGLSTTPC
                     TERVRRLERDGVILGYHARLDPPQLRAGLLVFVELSLDYKSGDTFEEFRRAVLRLPQV
                     LECHLVSGDFDYLVKARISEMASYRKLLGDILLKLPHVRESKSYIVMEEVKESLALPI
                     AD"
     gene            5449..>6091
                     /locus_tag="VIO83_03645"
     CDS             5449..>6091
                     /locus_tag="VIO83_03645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009314516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8330403.1"
                     /translation="MSPRLATHSAAHAPSWYAASANRALAFPALDGEVGVDVCIVGGG
                     YTGLNCAIELARRGYAVALLEARQIGWGASGRNGGQLIRGVGHEVERFLPLLGTDGVR
                     RLKLYGLQAVELVRQRITEFAIDCDLRWGYCDLANRPAHLEGFARARDELLALGYRHE
                     LRLLQAEQMHEVVGSQRYCGGLIDMGSGHLHPLNLALGEAEAAAGLGARLFERS"
BASE COUNT          943 a         2041 c         2141 g          966 t
ORIGIN      
        1 gtgccctaca ctgagcagcg agcaacgtcc gagcggagcg gccaggcctc atggaacagc
       61 tgaaacagaa gatccgcgag cacggcatcg tgctctccga gcaggtgctc aaggtggatg
      121 ccttcctcaa ccatcaggtc gacccgctgc tgatgcagca gatcggcgca gaactcgccc
      181 ggcgcttccg cgaccagggc atcagcaagg tgctcaccat cgaggcctcg ggcatcgccc
      241 cggcgctgat gaccgcgctg gcgctgggcg tgccgatgct cttcgcgcgc aagcagcgct
      301 cgctgacgct gaccgaccag ctactgaccg cgcgggtgta ctccttcacc aagcaggtgg
      361 agagcaccat cgccatcgcc gcccagcacc tcgccgccca cgaccgggtg ctgatcgtcg
      421 acgacttcct cgcccacggc caggccgcgc tggggctggt ttccatcgcc cagcaggccg
      481 gggccagcat cgccggcatc ggcatcgtga tcgagaagtc gttccagccc ggccgcgcgg
      541 agctggagag gctcggctac cgggtcgaat ccctggcgcg ggtcgcctcg ctgagcgggg
      601 gccaggtcca ctttgtcgag tgaaccggcc ccgcgcgccg cgccggtcag cgcgcccggc
      661 gcgtcgacga cttgcggctg gcgctacccg gcgcctgcgg ggtcagcagg tgcaggtagt
      721 cctgcaggta atcctgcagg gtggtgctga tcggcatggc gccggtgctt tccttcaggg
      781 ccgcggcact gtccttttgc cagctcgaca ccgcctcctg caccgccgcg ctgaaggcgc
      841 tctggttgtt gagggtggtt tccgccagct gctggacggc cccgcactgc agctgcaggg
      901 ccttccagcc cagatcgctg gcgacaaagc ccagcgagtt ccagtcgcgg gcgtcgagca
      961 tgcgggtgga cgcgctctcc agctcggcgg cgcaggcgtc gaccgcgcga ttgcccgcct
     1021 cgctccagcg caggccgttt tcccgcagca ggtcgcagag acggaagtag aactgcagat
     1081 tgaccttgta ctgggtcagc gaggattgcg catcgaggta catgacgacc tcctacaggg
     1141 tgaggcggat ggggaggaaa gccgaagcta cttttgccgc acgtactcgc ccggcgcgtc
     1201 agcgacgacc gggtagccct gctcgggcgc tccggtggcg ggagcggcaa ccggcgtcga
     1261 ggagcgcgcc tgcagccagg cgagccattc cagccaccac gagtcttcat ggcgcggggc
     1321 gctggcgagc caggcatcgg gggcgacgta attgccgccg accggccgct gcagcacctg
     1381 gtagtgccgc cgcggccggc ccggctcgct gacgatgccg gcgttgtggc cgccgctggt
     1441 cagcacgaag gtgatctcgg tggggctcag gtagtgcagc ttgaacaccg agcgccacgg
     1501 cgccacgtgg tcctgggtgg tggcgacgca gaacaccggg gtgtcgatgt cgctcagcga
     1561 caccggccgc ccacccaccg gatagcggcc ctcgctgagg tcgtcgttga ggaacaggcg
     1621 ccgcaggtac tggctgtgca tgcgcgccgg catccgcgtg gcatcggcgt tccaggccat
     1681 cagatcgatc atctcggcgc gctcgcccat caggtactcg ccgaccatgc gcgaccacag
     1741 cagatcgctg gagcgcagca gctggaaggc gccgaccatc tgcccggcgg tgagatagcc
     1801 ggtttcgtgc atctgcgcct cgagcaggct gacttcgctc tcgtcgatga acagcgccag
     1861 ctcgcccggc tcggtgaagt cggtctgcgc ggtgagcagg gagatcgacg ccaggcgctc
     1921 gtcgttgtcg cgggccatcg cggccgcggc gatggccagc agggtgccgc ccaggcagta
     1981 gccggtggcg tgcaccttgc gatcgggaac gatggcattg atcgcgtcga gggcggcgaa
     2041 gaagccgagc tgcaggtact cgtccatgcc caggtcgccg tcctcggccc ccgggttctt
     2101 ccaggacagg cagaacaccg tgtggccctg gtcgaccagg tacttgatca gcgagttgtg
     2161 cggcgacagg tcgaggatgt agtacttcat gatccacgcc ggcacgatca ggatcggctc
     2221 cggatgcacg gtggcggtgg tcggggtgta ctggatcagt tcgatcaggc ggttcttcag
     2281 caccaccttg cccggggtcg cggcgacgtc gcggccgacc acgaagttct cggtgccggc
     2341 cggtggatgg ccggcggcca ggcgttcggc atcttccagc gcgtggatca tgccgcgcac
     2401 caggttggcg ccgccggtcg cggcggtgcg ctgcagcacc agcggattgc tcgccaggaa
     2461 gttgccgggg gagaacaggt cgagcagctg gcgggccgcg aaggccacca cctcctcgtg
     2521 gtggcgcgag acgccgcgca cgccgtgggt cgccacctgc caccattcct ggctgagcag
     2581 gaacgactgg tgcatcaggc tgaacggcca ctggtgccat tccttggccg cgaagcggcg
     2641 gtcgcgccgc ggcggctcga cacactcgtg cgccggacag tccggcgagg cgctggcgac
     2701 ttcgcggaca taatcgagca ggcggcgggt ctgctccaga ccgagattca gcagttccag
     2761 ctgcttgccg ggcgaaccgg cgagatggcc ggaccagtcc atcagggcca acagcatgct
     2821 gatgggcgat accgaactgt tggcgcgggc gatcgccgcg tggaccttga ggtcgagggg
     2881 attaccggcg acacggggaa ccgagacggc cgggggagtc ataggctgca gggcactgat
     2941 gttcatctac cactcctcgc tggaaatccg cgcagcgcgc aggtcggcaa gcaggggggc
     3001 caagtgcgca gctggcggcg gtagcgcgaa gcaggccgca tcggggccgc catcgctgac
     3061 cggactgatc agtagcctag cactaaatca tggcagctgt gtgcactgca caagcacaaa
     3121 aaaaccgcct tgagcggcgg tttttttgcg acgcggcgaa cttacaggcc ggacatcttc
     3181 ttcagtgcac ccagcagctc gtcgtcggag cagtcggcgc aggtgccttt cggcggcatg
     3241 gcattgcggc caccgatggc gctcttcagc agcgcatcga cgccaccacg ctcggcggcg
     3301 cgggccttcc agtcggcagc atcgccgatc ttcggcgcat tcagcacgcc ggtactgtgg
     3361 caggcgccgc agttcttggc gatgatgtca tcggcgctgc gcgcgccgcc ggcagcggcc
     3421 gcaacagcac ccacgccctt gcactcctgg ccggcgatgc agacttcgcc taccggcttg
     3481 agacgctcgg cgatgttgtc atcgctatcg gcctgggcgc caaccgccca cagggccagc
     3541 acggcagcct gggctgccag gaatttcttg atcaggttca cccttacacc ctctttcgtg
     3601 gctagtcacg cccggggcga tggaccgcgc gggcgccgca gtataacggc aaagccgagc
     3661 cggccgaaac aaccccgatg gtcgcagggg cttgacagcg gcacgggcga acgccacgcc
     3721 gtccgctcag aacgcggccg gggtggtggc actgatcaac cgcgccggct gctcgaacgg
     3781 attgcggaaa cggtgcggct ggtcgctgcg aaagtagtag ctgtcaccgg tgtcgaggat
     3841 gaacacctcc tcgccgacgg tcagctcgag gcgcccatcg accagcatgc cggcctcctc
     3901 gccctcgtgg ctgagcattt cccggccggt gtcggcaccc ggcggatagg tttcgtcgag
     3961 gaacgccagg gtgcggttgg ggaaggcccg gccgaccagc ttcatggcca ccgcgccgct
     4021 gccgatgtcg atcagctcgt cggacttgta gaccacctgg gcctggtcgt cctgcgccac
     4081 gtccatggag aagaactcca ccagcgacat ggggatgccg cccagcacct tcttcagcga
     4141 gctgatcgag gggctgacgc tgttcttctc gatcatcgaa atggtgctgt tggtcacgcc
     4201 ggcccgcttg gccagctcgc gctgggacag gcccttgagt ttgcggatgg cgtacagacg
     4261 ggctccgacg tccaaggctg gcgtcctcct cgatcgttca ggctgatggg ggttgttgcg
     4321 tatagcatgg caacgccgtg cagtattttc aacaaagcgg ccgacagggc gcggcgcagc
     4381 accggcctgt cggggcacgc acgcttacgg cagggcggcg atcaccatca tctccacgcg
     4441 cacctccggc gcgtacaggc gggcctccac cgtggcgcgc gccggcgacg caccgggggc
     4501 gacccaggcg tcccacacct cgttcatgcc ggcgaaatcg gtggcgatgt cgcgcaggta
     4561 gatggtggtc gacaggatgc gggtcttgtc ggtaccggcc tcggccagca ggcggtcgat
     4621 gctggccagg gtttcgcggg tctgctggcg gatgtcgccg gacaggtcgt cggccagctg
     4681 cccgccgagg tggacggtgc cgttgtggat gacgatctcg ctgtagcgcg gctcgctgtg
     4741 caggcgccgg atggccatgg gggagtctcc tggtggtggc cccggcgagt gcggccgggg
     4801 cgggcatggg aagcgctgcg gcagtggctt tcactggcgg cgaggcgcta ttttgcgaca
     4861 aatcgcagag caaatgcgcc ccgggagggg catcccccat gagcagtccg cacaagtccc
     4921 gccgcgacct ggaccgcatc gaccgcaaca tcctgcgcat cctgcaggag gaggggcgga
     4981 tctccttcac cgaactgggc gagcgggtcg gcctgtcgac caccccctgc accgagcggg
     5041 tgcgccgcct ggagcgcgac ggggtgatcc tcggctacca cgcgcggctc gacccgccgc
     5101 agctgcgcgc cggcctgctg gtgttcgtcg agctgagcct ggactacaag tccggcgaca
     5161 ccttcgagga gttccgccgc gcggtgctgc gcctgccgca ggtactggag tgccatctgg
     5221 tgtccggcga cttcgactac ctggtcaagg cgcgcatctc ggagatggcc tcctaccgca
     5281 agctgctcgg cgacatcctg ctcaagctgc cgcacgtgcg cgagtcgaag agctacatcg
     5341 tcatggaaga ggtgaaggag agcctggcgc tgccgattgc cgactgattg gcgcgcgccg
     5401 gcggacgacc tatgctcggg acaatcgcca gccattgcgg acccgcccat gtccccgcgc
     5461 cttgccaccc atagcgccgc gcacgcgccc tcctggtacg ccgccagcgc caaccgggcg
     5521 ctggcgtttc ccgcgctgga cggcgaggtc ggcgtggatg tgtgcatcgt cggcggcggc
     5581 tacacgggct tgaactgcgc catcgagctg gcccgccgcg gctacgcggt ggcgctgctg
     5641 gaggcgcggc agatcggctg gggcgccagc gggcgcaacg gcggccagct gatccgcggc
     5701 gtcggccacg aggtcgaacg cttcctgccg ctgctcggca ccgacggcgt gcgccgcctc
     5761 aagctctacg gcctgcaggc ggtggagctg gtgcgccagc gcatcacgga gtttgccatc
     5821 gactgcgacc tgcgctgggg ctactgcgac ctggccaacc gccccgcgca cctggaaggc
     5881 ttcgcccgcg cccgcgacga gctgctggcg ctcggctacc gccacgagct gcgcctgctg
     5941 caggcggagc agatgcacga ggtggtcggt tcgcagcgct actgcggcgg gctgatcgac
     6001 atgggctccg gccacctgca cccgctcaac ctggcgctcg gcgaggctga agccgccgcc
     6061 ggcctgggcg cgcggctgtt cgagcgctcg g
//
LOCUS       DATCUN010000096         8432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_60120, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000096 DATCUN010000000
VERSION     DATCUN010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60120"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VIO83_03650"
     CDS             complement(<1..201)
                     /locus_tag="VIO83_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY8330404.1"
                     /translation="METRQVVIEDWDGDGGIRIPDEALQEMGVDVGDSLYLIEEYVGT
                     TRCLVLSKTPRIPDRVDELVDHW"
     gene            complement(522..1040)
                     /locus_tag="VIO83_03655"
     CDS             complement(522..1040)
                     /locus_tag="VIO83_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330405.1"
                     /translation="MKKTKLAVLLVAASLSPAWALAADPTPAKSSTPQAQQQAPNIAE
                     FDKQMAQAQENLQKMQAQMEKIRQTQDPKERQKLLQEHWNTMQGNMGMMHGMGMGMWG
                     GGMMGGHMMGGGMMGGGHMMGWQDYSNLPQEQKAQRQYMMDRYMGMQQMMMDHMMWHQ
                     HYMGMPPAKPAP"
     gene            complement(1177..1536)
                     /locus_tag="VIO83_03660"
     CDS             complement(1177..1536)
                     /locus_tag="VIO83_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013974883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEY8330406.1"
                     /translation="MKQVLVTILTATLVQTATAADMHDKPMDRMPMEQPMQVEQPDSA
                     APTAKATGTIKGIDAAKGTITLSHGPVPSLKWPPMTMGFTATPEQLKGLKVGDEVSFE
                     FLSQGMAARIISIQKAD"
     gene            complement(1533..4706)
                     /locus_tag="VIO83_03665"
     CDS             complement(1533..4706)
                     /locus_tag="VIO83_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019821050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8330407.1"
                     /translation="MIAALIRWSVANRFLVLLATLFVTAWGVWSVRSIPLDALPDLSD
                     VQVIVRTPYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGYSFFGDSYVYVLFEDGTD
                     LYWARSRVLEYLSQVQSRLPAGARPALGPDASGVGWIYQYALVDRSGRHDLAQLRALQ
                     DWFLKFELKTVANVAEVATIGGMVRQYQVLLDPTRLASLGITQAEVIAAIGQANRETG
                     GAVLEMAETEFMVRASGYLKTLDDFRAIPLRLSAGGVPVTLGDVASIQLGPEMRRGIG
                     ELDGEGETVGGVVILRSGKNARDTIAAVKAKLDELKGSLPAGVEIVTTYDRSKLIDRA
                     VANLGHKLLEEFIVVALVCALFLWHLRSSLVAIISLPIGVLIAFIVMRHQGINANLMS
                     LGGIAIAIGAMVDAAVVMIENAHKKVEAWHSAHPGETLAGERHWQVMTDAAAEVGPAL
                     FFCLLIITLSFIPVFTLEAQEGRLFGPLAFTKTYAMAAAAGVSVTLVPVLMGYWIRGR
                     IPREEHNPLNRWLIRLYRPALDAVLRRPKVTLLAALLVFLSALWPISRLGGEFLPPMD
                     EGDLLYMPSALPGLSAQKAAQLLQQTDRLIKTVPEVEHVFGKAGRAETATDPAPLEMF
                     ETTIQFKPREQWRPGMTPEKLVEELDRVVRIPGLTNIWIPPIRNRIDMLATGIKSPIG
                     IKVAGTSLVDIDAATQAIERVAKSVPGVSSALAERLTGGRYVDVDIDRRAAARYGLNI
                     ADVQSIVASAIGGENVGETVEGLARFPINVRYPREWRDSLGALEQLPIYTPQGNQITL
                     GTVARIAVSDGPPMLKSENARPSGWVYIDVRGRDIAAVVGDLRRTVSEKVSLQPGMSI
                     SYSGQFEYLERANARLKLVVPATLAIIFVLLYLTFARVDEALLIMATLPFALTGGVWF
                     LYLLDYDLSVATGVGFIALAGVAAEFGVIMLLYLNQAWAEREAAGERSEQALLAAIRQ
                     GAVQRVRPKAMTVAVIVAGLLPIFWGSGTGSEIMSRIAAPMVGGMLSAPLLSLFVIPA
                     AYRLMRRSHLLTASAVKPSNSTGATQS"
     gene            complement(4703..6169)
                     /locus_tag="VIO83_03670"
     CDS             complement(4703..6169)
                     /locus_tag="VIO83_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007981985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8330408.1"
                     /translation="MSMRIGPAAALCGLALVLGAGGGYWFAQQRPAPIPVPAAQDERP
                     VLYWYDPMYPQQRFDKPGKSPFMDMPLVPRYADAAADSAAVSIDPRLTQNLGLRLATV
                     TRGAIASSLEVTGVLAFNERDVAVVQARTAGFVERVYARAPDDVLAAGAPLADLLVPE
                     WAAAQEEFLALRRSGDAELLAAARQRLRLTGMPATLIARVERSGKAQPTLTITSPIGG
                     VLQELAVRTGMSMAAGDTLARINALDSVWLELAVPEAEAGALLADQTVEARLPALAGE
                     VLTGRVATILPAANTDSRTLRVRVELPNPDGRLRPGLTAQVRLSRASEQGVLWVPSEA
                     VIRTGRRALVMLAEDAGRFRPVEVRPGAESYGRTVVLSGLEEGQQVVASGQFLLDSEA
                     SLRGLVVGTVARPEPATNTPVLHEAEGRVVEIGDGSVTLAHGPFRTLGMPGMTMSFPL
                     GNPALLHGLEVGDRVRFAVRESDAGLVVERLEKQGDQP"
     gene            complement(6166..7422)
                     /locus_tag="VIO83_03675"
     CDS             complement(6166..7422)
                     /locus_tag="VIO83_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY8330409.1"
                     /translation="MNIPRHCPGWPLAAALAASVLALPSQAASLTLAEALRLAEDNAP
                     SLAAEQAKLQAASSAAIPAGELPDPQLRLGVQNYPVGGPDRWSIDRDFMTMQMVGVMQ
                     DMPNSAKRRARVEVADAAVERAAAESAVERLNVRQATALAWIDRLAVERQQALFTEFF
                     GENRLLAAAVQAQIAGGRGQAADAVRPKQEAARLDEQQDQLAQQHVRARAALRRWIGV
                     AADQPLAGDWPEWPIDHARYGHQLPRHPALASFEPMTREAQAKVREAQAEKSSDWSWE
                     LDYQRRGREFGDMVSVQVSFDLPLFPGSRQDPKIATRHAELNQLEAEREALLREHAQQ
                     LEEDLAEYQRLQRALHRNRNSFVPLAREKVELSLASYRAGKGDLLSVITARQELIEAR
                     LRQIELEAKGAKTAARLHFAYGEEHP"
     gene            complement(7514..7864)
                     /locus_tag="VIO83_03680"
     CDS             complement(7514..7864)
                     /locus_tag="VIO83_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009048575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330410.1"
                     /translation="MKRHLRLCLVFLLSLTLPLTGMAGVQPPAEPCPMQATAMAAMTD
                     MAVVPPDCCQDMGDPAEPGKPCKPGQECKTASLLQVAVIKPPVPLSAPIAASYTSDLL
                     PERPPSGLWRPPCA"
     gene            complement(7952..>8432)
                     /gene="cadR"
                     /locus_tag="VIO83_03685"
     CDS             complement(7952..>8432)
                     /gene="cadR"
                     /locus_tag="VIO83_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Cd(II)/Pb(II)-responsive transcriptional
                     regulator"
                     /protein_id="HEY8330411.1"
                     /translation="KIGQLARTTGTQVETIRYYEREGLLASPARSDGNFRIYTEAHVE
                     RLSFIRRCRMLDMTHAEIRGLLHFKDAPSEHCGDVNALLDAHIGHVTARIEELRQLEQ
                     QLKSLRECCNEPSTAAHCGILHELSQPLCEGATSEGNHLRCAHPGGPSHQRQTGTTQ"
BASE COUNT         1353 a         2710 c         2802 g         1567 t
ORIGIN      
        1 ccagtggtca accagctcat cgacgcgatc aggaatgcgc ggggttttgc tgagcacaag
       61 gcagcgtgtg gtgcccacgt actcctcgat caggtacagc gagtccccga cgtcgacccc
      121 catttcctgc agggcttcat caggaatgcg aataccgcca tcgccatccc agtcttcgat
      181 cacgacctgt cgggtttcca tgggtggctc ctgggtgagt tagatcaggc gtccgcgggg
      241 ggaggcgacg gcgagccgac gcccacggat cccacggcac aggcgttcag ggtagcgaag
      301 cggccgccgg ccgaaaagtg acgataacgg attttatcgt cactttccgg ccatgggagg
      361 gaaccttggg ctatgcacta aaagtgcctg tgctcggtga ggtcgcgttt aaaagtggct
      421 cggaatgccc cattagggcc agtacactcc gcgccatcgt cacttttcgt cttgcgtgac
      481 cttcgttcag ctgacttttc ggacagaacc cagagtcatg cttacggagc gggtttagct
      541 gggggcatcc ccatgtagtg ttggtgccac atcatgtgat ccatcatcat ctgctgcatc
      601 cccatgtacc tgtccatcat gtactgacgc tgtgctttct gctcctgggg caagttcgag
      661 tagtcctgcc agcccatcat gtggccgccc cccatcatgc cgccacccat catgtgcccg
      721 cccatcatgc cgcctcccca cattcccatg cccatgccgt gcatcattcc catatttcct
      781 tgcatcgtat tccaatgctc ctgaaggagt ttttgccgtt ccttcgggtc ttgggtctga
      841 cggattttct ccatctgggc ctgcatcttc tggaggtttt cctgcgcttg agccatctgt
      901 ttgtcgaact cagcaatatt cggagcttgc tgctgcgcct ggggcgtaga acttttggct
      961 ggggttgggt ctgccgccag cgcccatgcg ggtgacagtg atgcggctac gaggagtact
     1021 gccagcttgg tttttttcat gtcgagctgc ctttgtggtg gtgagctgaa ccaacatgcc
     1081 tacgagccgt agtgattacg ttgacaacat ggctttcgtc tagaacttta gcgtcccttg
     1141 gctgggtttc cccatcgtga gtcaagggga ataggctcaa tcagctttct gaatggaaat
     1201 gatccgggcc gccatgcctt ggctcaggaa ttcgaagctc acttcgtcac cgaccttcag
     1261 ccccttcaac tgctcgggcg ttgcggtaaa gcccatggtc attggtggcc atttcaagct
     1321 gggaacgggg ccgtgcgaaa gcgtgatggt ccctttggca gcgtcgatcc ccttgatcgt
     1381 gccggtggcc ttggcggtcg gggccgcgct gtccggctgt tccacctgca tgggctgttc
     1441 cattggcatc ctgtccatgg gcttgtcgtg catgtcagcc gctgtggcgg tctggacgag
     1501 cgtggcggtc aggatggtta cgaggacttg tttcatgact gggttgctcc tgtggaattg
     1561 gatggtttca cagccgatgc tgtgaggaga tgcgagcggc gcatcagccg ataggccgcc
     1621 gggatgacga acagggacag caagggcgcg ctgagcatgc cgccaaccat gggagcggcg
     1681 atgcggctca tgatctcgct gccggtgccg ctgccccaga agatcggcag caggccggcg
     1741 acgatcaccg ccacggtcat cgccttgggc cgcacgcgct gcacggcgcc ctggcgaatc
     1801 gccgccagca gtgcctgctc gctgcgctcg ccggccgcct cgcgctcggc ccaggcctga
     1861 ttcaggtaga gcagcatgat cacaccgaac tcggcggcca ccccagccag ggcgatgaag
     1921 ccgacgccag tggccaccga caggtcgtag tccagcaggt agaggaacca cacgccgccg
     1981 gtgagggcga atggcagcgt cgccatgatc agcagggcct cgtcgacgcg ggcaaaggtc
     2041 aggtagagca gcacgaagat gatcgccagg gtcgccggca ccaccagctt gagccgggcg
     2101 ttggcccgtt cgaggtactc gaactgccca gaatagctga tgctcatgcc tggctgcagg
     2161 ctcacctttt cgctgacggt ccggcgcagg tcacccacca cggcagcgat atcccgacca
     2221 cgcacatcga tataaaccca acccgatggc cgggcgttct cgcttttcag catgggcggt
     2281 ccgtcgctga ccgcgatcct cgccaccgtg cccagagtga tctggttgcc ttgcggggta
     2341 tagatcggca gctgctcgag agcgccgagc gagtcgcgcc actcgcgtgg atagcgcaca
     2401 ttgatcggga agcgggccag gccctcgacg gtctcgccga cgttttcgcc gccgatggcg
     2461 ctggccacta tcgactgcac gtcggcgatg ttcagcccgt agcgggcagc cgcgcgacgg
     2521 tcgatgtcca catcgacata gcgccctccg gtcaggcgct cggccagcgc cgagctcact
     2581 ccgggcacgc tcttggccac gcgctcaatc gcctgggtgg ctgcgtcgat atccaccagg
     2641 cttgtgccgg cgaccttgat cccgatgggg ctcttgatcc cggtggcgag catgtcgatg
     2701 cgattgcgga tcggcggaat ccagatgttg gtgagccctg ggatccgcac cacccgatcc
     2761 agctcttcca ccagtttttc cggcgtcatg cctggtcgcc actgctcgcg cggcttgaac
     2821 tggatggtgg tttcgaacat ctccagcggt gccgggtcgg tggcggtttc ggcacgtccg
     2881 gccttgccga acacgtgctc gacttccggt accgtcttga tcaggcgatc tgtctgctgc
     2941 aacagctgcg cggccttttg cgccgacagt ccgggcagcg ccgaaggcat gtagagcaag
     3001 tcgccttcgt ccatcggcgg caggaactcg ccgccgaggc gggaaatcgg ccacagtgcg
     3061 ctgagaaaca ccagcagcgc agccaacagg gtgactttgg gccggcgcag cactgcatcc
     3121 agagccggcc ggtagaggcg gatcagccag cgattcagcg ggttgtgctc ctcccggggg
     3181 atccgcccgc gaatccagta gcccatcagt accggcacca gggttaccga caccccggcg
     3241 gcggccgcca tggcgtaggt cttggtgaag gccagcgggc cgaacaggcg gccttcctgg
     3301 gcctccaggg tgaataccgg gatgaacgat agggtgatga tcagcagaca gaagaacagc
     3361 gccgggccga cctcggcggc ggcgtcggtc atcacctgcc agtggcgctc gcccgccagc
     3421 gtctccccgg ggtgagccga gtgccaggct tcgaccttct tgtgagcgtt ctcgatcatc
     3481 accaccgccg catccaccat ggctccgatg gcaatggcga tgccgccgag tgacatgagg
     3541 ttggcgttga tgccttggtg gcgcatcacg atgaacgcga tcagcacgcc aatcggcagc
     3601 gagatgatcg ctaccaggga tgagcgcagg tgccagagga acagcgcgca gaccaaggcg
     3661 acgacgatga actcctcaag gagcttgtgg ccgaggttgg ccaccgcgcg gtcgatcagc
     3721 ttgctgcggt cgtaagtggt gacgatttcc accccggcgg gcaggctgcc cttcagctcg
     3781 tcgagtttgg ccttgaccgc agcgatcgtg tcgcgggcgt tcttgccgct gcgcaggatc
     3841 accacgccgc cgaccgtttc gccttcgccg tcgagttcgc cgatgccgcg gcgcatctcc
     3901 gggcccagct ggatgctcgc cacgtcgccg agggtcaccg gtacaccgcc ggcactgagc
     3961 ctgagcggga tggcgcggaa atcgtcgagc gtcttcaggt agccggaggc gcgcaccatg
     4021 aactcggtct cggccatttc cagcaccgcg ccgccggttt cccggttggc ctggccgatg
     4081 gccgcgatca cctcggcctg ggtgatgccc aggctggcca gcctggtcgg gtcgagcagg
     4141 acctggtact gcctgaccat gccgccgatg gtggccacct ctgcgacgtt ggcgacggtc
     4201 ttcagctcga acttgaggaa ccagtcctgc agggcgcgca gctgcgccag gtcgtggcgg
     4261 ccactgcgat cgaccagggc gtactggtag atccagccga ccccgctggc atccggtccc
     4321 agcgccggcc gggcgcctgc cggtaggcgg ctctgcacct ggctcaagta ctccagcacc
     4381 cgcgagcgcg cccagtagag gtcggtgccg tcctcgaaca gcacgtagac gtagctgtcg
     4441 ccgaagaagg agtagccgcg taccgttttc gctcccggca ccgagagcat ggtggtggtc
     4501 agtggatagg ttacctggtt ctcgacgatc tgcggcgcct gccccggata gggggtgcgc
     4561 acgatcacct gtacatcgga gaggtccggc agtgcgtcga gcgggatgct gcgcaccgac
     4621 cagacgcccc aggcggtgac gaacagggtt gccagcagga ccaggaagcg gttggctacc
     4681 gaccagcgga tcagcgcggc gatcatggct ggtctccctg tttctccaag cgctcgacca
     4741 ccagcccggc gtcgctctcg cgcaccgcga agcgcacccg gtcgccgacc tcgagtccgt
     4801 gcaacagggc cggattgccc agtgggaagc tcatggtcat gccgggcatc cccagtgtcc
     4861 ggaaaggacc atgggcgagg gtcacgctcc cgtcgccgat ctcgacgacc ctgccctcgg
     4921 cctcatgcag gacgggggtg ttggtggcag gctccggtct tgccacggtt cccacaacca
     4981 gccctcggag actggcttcg gaatcgagta ggaactgtcc ggaggcgacc acctgttggc
     5041 cttcctccag gccgctcagt accaccgttc ggccgtagct ttccgcaccg gggcgcacct
     5101 cgacggggcg gaagcgccct gcgtcttcgg cgagcatgac cagagcccgt cggccggtgc
     5161 ggatgacggc ctcgctcggc acccacagca cgccttgctc gctcgcccgg ctcagccgta
     5221 cctgcgccgt cagccccggc cgcaggcgtc cgtcggggtt gggcaattcg acccgcacgc
     5281 gaagggtgcg actgtcggtg ttcgccgctg gcaggatggt ggcgacccgg cccgtcagca
     5341 cctcgccagc gagcgccggc aggcgcgcct cgaccgtctg gtcggcgagc agtgccccgg
     5401 cttcggcctc gggcacggcc agctccagcc agacgctgtc cagagcgttg atgcgcgcca
     5461 gggtatcgcc ggccgccatg ctcatgcccg tccgcacagc cagctcctgc aacacgccac
     5521 cgatggggct ggtgatggtc agggtcggct gggccttgcc gctgcgctcc acccgggcga
     5581 tcaacgtcgc tggcatgccg gtcaggcgca ggcgctggcg ggccgccgcc agcagctcgg
     5641 catcgccgct gcgccggagg gcgagaaact cttcctgggc agcggcccac tcgggcacca
     5701 gcaggtcggc cagcggcgca ccagccgcca gtacgtcgtc tggtgcgcgg gcgtagaccc
     5761 gctcgacgaa gccggcagta cgcgcctgaa ccactgcgac atcccgttca ttgaaagcca
     5821 gcacgccggt tacctccaga ctggaggcga tggccccacg ggtgacggtc gccaggcgca
     5881 ggccgagatt ctgggtcaac cgcggatcga tgctgacggc ggcgctgtcc gcggcggcat
     5941 cggcatagcg aggcaccagc ggcatgtcca tgaagggcga cttgcccggc ttgtcgaacc
     6001 gctgctgcgg gtacatcgga tcgtaccagt acagcaccgg acgttcgtcc tgcgccgcgg
     6061 gcacgggtat cggagcaggg cgctgctggg cgaaccagta ccccccgcca gcgccgagaa
     6121 cgagcgccaa gccgcacaac gcggctgccg ggccaattcg catgctcatg ggtgctcctc
     6181 cccgtaggca aaatgcaacc gtgcagcggt cttggcgccc tttgcctcca gttcgatctg
     6241 gcgcaggcga gcttcgatca gctcctgacg ggcggtgatg acgctgagca gatcgccctt
     6301 gccggcgcgg tagctggcca ggctgagctc caccttctcc cgggccagtg gcacgaagct
     6361 gttgcggttg cgatgcagtg cccgctgtag tcgctggtat tcggcgagat cctcttccag
     6421 ttgctgggca tgttcgcgta gcagggcttc gcgctcggct tccagctggt tgagttcggc
     6481 gtgacgggtg gcgatttttg gatcctgccg agagccaggg aacagtggca agtcgaaact
     6541 gacctgcaca ctgaccatgt cgccgaactc gcggccacga cgctggtagt cgagctccca
     6601 gctccagtcg gagctcttct ccgcctgcgc ctcgcgcacc ttggcttgcg cttcgcgcgt
     6661 catcggctcg aaggaggcca gcgccgggtg gcgtggcaac tggtggccgt agcgagcgtg
     6721 atcgatgggc cattctggcc agtcgccagc aagcggctgg tcggcggcca cgcctatcca
     6781 gcgacgtagg gccgcgcgcg cccgaacgtg ttgctgggct agttggtcct gctgttcgtc
     6841 caggcgggcc gcctcctgtt tcggccgcac tgcgtcggca gcttgcccgc gtccgccggc
     6901 gatctgcgcc tgcacggcgg cagcgagcag acggttctcg ccgaagaact cggtgaacag
     6961 cgcctgctgg cgttccaccg cgaggcggtc gatccacgcc agcgcggtgg cctggcggac
     7021 gttcagtcgt tcaacagcgc tttcggcggc ggcgcgctct accgccgcat cggctacctc
     7081 gacccgagcc cggcgcttgg cgctgttggg catatcctgc atgactccga ccatctgcat
     7141 ggtcatgaaa tcgcggtcga tgctccagcg gtcggggccg ccgacgggat agttctgcac
     7201 ccccagccgc agctggggat cgggcagttc gccggcggga atggcggcac tactggcggc
     7261 ttgcagtttg gcctgctcgg cggccagcga aggggcgtta tcttcagcca ggcgcaaggc
     7321 ctcggcgagg gtcagcgagg ctgcctggct cggcaatgcc agcacggacg ccgccagcgc
     7381 ggcggcgagg ggccagcccg gacagtgacg tgggatgttc atgctctcga ttcctgtgat
     7441 gttccgtgtc gcgcgaacgc gcgcagccct gctgtcctga tcaggcggga tagcgttcag
     7501 tgtggggcag gaatcaggcg cagggtggtc gccagagccc ggatgggggg cgctcgggca
     7561 ggagatcgct ggtgtaggat gccgctattg gggcggacaa ggggacgggg ggcttgatga
     7621 ctgcaacttg cagcaggctg gcggtcttgc attcctgccc cggtttgcag ggtttaccag
     7681 gctcggcggg gtcgcccatg tcctggcagc agtccggagg cacgaccgcc atgtcggtca
     7741 tcgccgccat ggcggtcgcc tgcatcggac agggttctgc cggcggctgg actcccgcca
     7801 ttcccgtgag gggaagcgtc agactgagca ggaaaaccag gcagagccgc aggtggcgtt
     7861 tcatggacgg gagtctagtc ctgcacggtg gggtacacaa ttgcaggcgc accactcggc
     7921 acagcagtta aggagcttcg agcagagctc gttattgcgt ggtcccggtt tgccgctgat
     7981 gggaggggcc gcccggatgt gcgcagcgca ggtgattgcc ctcgctcgtt gcgccttcgc
     8041 acaacggctg cgacaactcg tggaggatgc cgcaatgggc ggcggtactc ggctcgttgc
     8101 agcattcacg caagctcttg agctgttgtt ccagctggcg gagctcctcg atgcgcgcgg
     8161 tgacatggcc aatgtgcgcg tccagcaggg cgttcacatc gccacagtgt tcgctggggg
     8221 catccttgaa atggagcagt cctcgaatct cggcgtgcgt catatccagc atgcggcaac
     8281 ggcggatgaa tgacagcctt tcgacatgcg cttcggtgta gatgcgaaag ttgccgtcgc
     8341 tgcgtgctgg cgaggcgagt agcccctcgc gctcgtagta gcggatggtt tcgacctggg
     8401 tgcccgtagt tctggccagt tgaccgattt tc
//
LOCUS       DATCUN010000097         4801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_154529, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000097 DATCUN010000000
VERSION     DATCUN010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154529"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VIO83_03690"
     CDS             complement(<1..364)
                     /locus_tag="VIO83_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330412.1"
                     /translation="MSNLYEVAFAGELVAGADAAQVRANLARLFQADPQRIAALFSGR
                     RTVIKSNLDAASAEKYRLTLERAGAQVEVSPVGQAPAAPAAPASAAPVAPVATAAVSQ
                     GVALPALKVAPRDEYMAAF"
     gene            490..990
                     /locus_tag="VIO83_03695"
     CDS             490..990
                     /locus_tag="VIO83_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016851905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEY8330413.1"
                     /translation="MIFPASIIRTLGLLLLLLAGHGLSGCASAPSDTVFKNPDVQLVN
                     VELVRARLLEQEFRLRFRIDNPDNVDLSVRSLKYTVFLEKVKLASGKYSTWLTVPARS
                     YREFDVPVNTNLWRQMKDIAKLLKKPDNPITYRLQGDLTVGLMFRHHVNIKRSGTIVP
                     GAFIPE"
     gene            994..1188
                     /locus_tag="VIO83_03700"
     CDS             994..1188
                     /locus_tag="VIO83_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY8330414.1"
                     /translation="MTQQDHVHGPDCNHDHDHYHEPHVHGPHCNHEHQEPVRNPLKDV
                     GRNDPCPCGSNKKYKKCHGA"
     gene            1196..1660
                     /locus_tag="VIO83_03705"
     CDS             1196..1660
                     /locus_tag="VIO83_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2489 domain-containing protein"
                     /protein_id="HEY8330415.1"
                     /translation="MSALSIGLLLAGLLVVLALGGYALHLWRRVWAQQKAYASAESAR
                     QQRLGGDLRILAGSLLDEQLPLIEGAIRIKVLLDNYNIQLSNHAHCRVFHTLFDATSG
                     IPTHAAWKALTAAERRQHDKRFSELELQHKAAARKAARWLLDEGLQQHPAHV"
     gene            1783..4323
                     /locus_tag="VIO83_03710"
     CDS             1783..4323
                     /locus_tag="VIO83_03710"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY8330416.1"
                     /translation="MSLRSFRPLAPLAGAAALTTLVSLSGCQTWLDSRYQDSLPPSSG
                     VQPLKGLADTVSVRRNALGMPLIESRSFHDALFALGYVHASDRLSQMVGLRLMAQGRL
                     AEMAGPGVLEIDRFMRAVNLRQSADIVYKNASPRLHKFFEVYARGVNAYLFRHRDKLP
                     MDLAASGYRPEYWKPEDSALIFCLLNFGLSVNLQEEISSLALAQKVGAEQLAWLLPTY
                     PDEPLPVDEAAKLKGLSLSGKIPGLAALDGVAGQLAALNMLGVAASNNWAIAPQRSRS
                     GKSLLANDPHLPLTQPSLWSFVHLRTPKYQAAGVSVAGIPAVVAGFNGKLAWGMTMVM
                     GDTQDLFLEQVRHQGGRLEYLADGQWQPARAHQETFFVKGQGPVRETFYETRNGPLLN
                     SVLGERKHAMQPIALASGYGLALKTNQFEADQTLDAVFDLSRAQSVEDAHEPVRNIRA
                     LGLNMLYADAQHIAWQVTGRYPNRKGGRGLVPSPGWDSQYGWDGYADTMLHPYDQDPM
                     QGWLGTANQRSVPAGYGVQLSNSWFYPERAERIAELAGASARHDARNMIAMQYDQQTP
                     FAAKLKAMFADPLMAQPLQRAIEALPAGERDQAREALKRLQAFDGQLAAGSADAALYA
                     MFLQESARQTFLDELGPEDSPAWQGLVEIAKLSYSAQADHLLGRVDSPFWDDVRTAQK
                     EDKPAILARSLAASVAQLESRLGSNRSDWQWGKLHRYQWDSDSSRLAPYMSAGQRASL
                     ATIKDYLDRGPYPAGGDHSTLNVSAWLWGKNFDTWLVPSMRIVVDFGQDEPLQAVNSS
                     GQSGNPASKHYADGIDAWLKGRYLSFPFQSHNLDRSYGSQRLLLTPGR"
     gene            4447..>4801
                     /locus_tag="VIO83_03715"
     CDS             4447..>4801
                     /locus_tag="VIO83_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330417.1"
                     /translation="MPTERAAQLERYRRNLQGEIDSAALYRALSRCESRPELQELYRR
                     LADIEERHVGFWRQQFQQLGASAPSLRPGWRTRLLIRIARLLGPDWVVGAAASLEQID
                     RCQYDDQPETCATGMP"
BASE COUNT          842 a         1768 c         1471 g          720 t
ORIGIN      
        1 tgaatgccgc catgtattcg tcgcgcggcg ccaccttgag tgcgggcaac gccacgccct
       61 gggacacggc ggccgtcgcg accggggcca ccggcgcagc tgacgcaggc gcagcaggtg
      121 ccgcaggcgc ctgaccgacc ggactgacct ccacctgagc gccggcgcgc tcgagggtca
      181 ggcgatactt ttcggcgctg gccgcatcca gattgctctt gatcaccgtg cgccgtccgg
      241 aaaacagcgc ggcaatccgc tgcggatcgg cctggaacag acgcgccagg ttggcccgca
      301 cctgcgccgc atcggctccc gccaccagct cgcccgcgaa ggctacttca taaagattgc
      361 tcaaccgtca cactcctgtc ctttttcctg cgcagtatgg cgctggtttt tttcctgcaa
      421 cattcccgca acaacacgtt gcagcccttg ttgcgattgt tacactcggg catccaacgg
      481 agtcttggca tgatctttcc ggcgtccata ataagaaccc tcggcctctt gctgcttctg
      541 ctcgccggtc atggcctgag cggctgcgcc agcgcgccga gcgacactgt attcaagaac
      601 cccgacgtcc aactggtcaa tgtcgaactg gttcgcgcgc gcctgctcga gcaggagttc
      661 cgcctgcgct ttcgcatcga caaccccgac aacgtcgacc tctcggtacg cagcctcaaa
      721 tacacggttt tcctcgagaa ggtgaagctg gcaagcggca aatactcgac ctggctcacg
      781 gttccggccc gcagctatcg ggaattcgac gtaccggtga acaccaacct ctggcggcag
      841 atgaaagaca tcgccaaact attgaagaag cccgacaatc cgatcaccta tcgcctccag
      901 ggcgatctga cggtcgggct gatgttccgc caccacgtca atataaaacg tagtggcacc
      961 attgttcccg gcgccttcat tccggagtaa gtgatgaccc agcaagacca tgtgcatggc
     1021 cccgactgca accacgacca tgaccactac cacgagccgc acgtccacgg cccgcactgc
     1081 aaccacgagc accaggagcc ggtgcgcaat ccgctgaagg atgtcggccg caacgatccc
     1141 tgcccgtgcg gcagcaacaa gaagtacaag aagtgccacg gcgcctgagc cgagcatgag
     1201 cgcactgtcc atcggtctgc tgctggccgg cctgctcgtc gtgctggctc tgggcggcta
     1261 tgccctgcac ctgtggcgcc gggtgtgggc ccagcagaag gcctatgcga gcgccgaaag
     1321 cgcacgccag caacgcctcg gcggcgatct gcgcatcctt gccggcagcc tgctcgacga
     1381 gcagctgccg ctgatcgaag gcgccatccg catcaaggtc ctgctcgaca actacaacat
     1441 ccagctcagc aaccacgcgc attgccgggt cttccacacc ctgttcgacg caacctccgg
     1501 catccccacc catgccgcat ggaaggcact gacggccgcc gaacgccgcc agcacgacaa
     1561 gcgcttcagc gaactcgaac tgcaacacaa ggctgccgcc cgcaaggccg cccgctggct
     1621 gctcgacgaa ggcctgcagc agcaccccgc ccacgtctga aagagccggc cccgacagct
     1681 cttgtcaggc gcctgccgtc cccctaacgt agcgcccttt tccggcatgg ctccttgcgc
     1741 tgtgctgaac tggtcttgcc tgcccgctcc tggagcctcg ccatgtcgct gcgttcgttt
     1801 cgtcccctcg ccccgctggc gggtgccgcc gccctgacca cgctggtcag cctgagcggc
     1861 tgccagacct ggctggacag ccgctaccag gacagcctgc cgcccagctc cggcgtgcag
     1921 ccgctcaagg ggctggccga cacggtgtcg gtgcggcgca acgcgctggg catgccgctg
     1981 atcgagagcc gcagcttcca cgacgcgcta ttcgccctcg gttacgtgca cgccagcgac
     2041 cgcctgagtc agatggttgg cctgcgcctg atggcccagg gccgcctggc cgagatggcc
     2101 ggccccggcg tgctggagat cgaccgcttc atgcgcgcgg tgaacctgcg ccagagcgcc
     2161 gacatcgtct acaagaacgc ctcgccgcgc ctgcacaagt tcttcgaggt ctacgcccgc
     2221 ggcgtcaacg cctacctgtt ccgccatcgc gacaagctgc cgatggacct ggccgcctcc
     2281 ggctaccgcc cggagtactg gaagccggaa gactcggcgc tgatcttctg cctgctcaat
     2341 ttcggcctgt cggtaaacct gcaggaagaa atctccagcc tggcgctggc gcagaaggtc
     2401 ggcgccgagc agctggcctg gttgctgccg acctacccgg acgagccgct gccggtcgac
     2461 gaggcggcca agctcaaggg cctcagcctg agcggcaaga tcccggggct cgccgcgctg
     2521 gatggcgtcg ccggtcaact ggcggcgctg aacatgctcg gcgtcgccgc ctccaacaac
     2581 tgggccatcg ccccgcagcg cagccgcagc ggcaagagcc tgctggccaa cgacccgcac
     2641 ctgccgctga cccagccctc gctgtggagc ttcgtgcacc tgcgcacgcc caagtaccag
     2701 gccgccggcg tctcggtggc cggcatcccc gcggtggtcg ccggcttcaa cggcaagctg
     2761 gcctggggca tgaccatggt catgggcgac acccaggacc tgttcctcga acaggtgcgc
     2821 caccagggcg ggcgcctcga gtacctcgcc gacggccagt ggcagccggc gcgcgcgcac
     2881 caggaaacct tcttcgtcaa gggccagggt ccggtccgcg aaaccttcta cgaaacccgc
     2941 aacggcccgt tgctgaacag cgtgctcggc gaacgcaagc acgccatgca acccatcgcc
     3001 ttggccagcg gctacggcct cgccctgaag accaaccagt tcgaggccga ccagaccctc
     3061 gacgccgtct tcgacctgtc gcgcgcccag tcggtggagg atgcccacga gccggtgcgc
     3121 aacatccgcg ccctcggcct gaacatgctg tatgccgacg cccagcacat cgcctggcag
     3181 gtcaccggtc gctaccccaa ccgcaaaggc ggccgcggcc tcgtgccctc gcccggctgg
     3241 gacagccagt acggctggga cggctacgcc gacaccatgc tgcatcccta cgaccaggac
     3301 ccgatgcagg gctggctggg caccgccaac cagcgcagcg tgccggccgg ctacggcgtg
     3361 cagctgtcca actcctggtt ctatcccgag cgggccgagc gcatcgccga actggccggt
     3421 gccagcgcgc gccacgatgc gcgcaacatg atcgccatgc agtacgacca gcagacgccg
     3481 ttcgccgcca agctcaaggc catgttcgcc gatccgctca tggctcagcc gctgcaacgg
     3541 gccatcgagg ccctgccggc cggcgagcgc gaccaggcgc gcgaagccct caagcgcctg
     3601 caggccttcg acggccaact ggctgccggc tcggccgacg ccgcgctgta cgcgatgttc
     3661 ctccaggaaa gcgcgcgaca gaccttcctc gacgaactgg gaccggagga cagcccagcc
     3721 tggcaaggcc tggtggaaat cgccaagctc agctactcgg cgcaggccga ccacctgctc
     3781 ggccgcgtcg acagcccgtt ctgggacgac gtgcgcacgg cgcagaagga ggacaagccg
     3841 gcgatcctcg cgcgcagcct ggccgccagt gtggcccagc tggaaagccg gctgggcagc
     3901 aatcgcagtg actggcagtg gggcaagctg caccgctacc agtgggacag cgacagctcc
     3961 cgtctggcgc cgtacatgag tgccggccag cgcgccagcc tggcgaccat caaggactat
     4021 ctggatcgcg gcccctatcc ggccggcggc gaccacagca ccctgaacgt ctccgcctgg
     4081 ctgtggggca agaacttcga cacctggctg gtgccgtcca tgcgcattgt ggtcgacttc
     4141 ggtcaggacg agccgctgca ggcggtcaac agctccggcc agtccggcaa cccggccagc
     4201 aagcactacg ccgacggcat cgacgcctgg ctcaagggcc gctacctgag cttcccgttc
     4261 cagagccaca acctcgaccg cagctacggc agccagcgcc tgctgctgac gccggggcgg
     4321 taggcgagca cggacaggtg gcgcgcacgg cgcaccctgc tggcgactct cgtagggtgc
     4381 gccgtgcgca ccagggcatt cacaacaggg gctctgtagg ggtattcccg catcggaggc
     4441 cagcgcatgc ccaccgaacg agcggcccag ctcgaacgct accgccgcaa cctgcagggc
     4501 gagatcgaca gcgccgcgct gtaccgcgcc ctttcccgct gcgagagccg ccccgagctg
     4561 caggaactgt accggcgcct ggcggacatc gaggaacgcc atgtcggctt ctggaggcag
     4621 caattccagc aactcggcgc cagcgcgccc agcctgcgcc ccggctggcg tacccgcctg
     4681 ctgatccgca tcgcccgcct gctcggcccg gactgggtgg tcggcgccgc cgcctcgctg
     4741 gagcagatcg accgctgcca gtacgacgac cagccggaaa cctgcgctac cggcatgccc
     4801 g
//
LOCUS       DATCUN010000098        10396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_60189, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000098 DATCUN010000000
VERSION     DATCUN010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10396
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60189"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1328)
                     /locus_tag="VIO83_03720"
     CDS             complement(147..1328)
                     /locus_tag="VIO83_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8330418.1"
                     /translation="MNSVSTRATLGTAALLSLLLLAGCQKEAAAPQARPAPQVGVVTL
                     ETQPFTLTTEVPGRTAAYRVAEVRPQVDGIVHKRLFKEGSEVKAGQQLYQIDPATYEA
                     TLKSAEASVLSSRSLAERYQVLVADQAVSRQQYDEARAAQLQAEAALEKARIDVRYTK
                     VLAPIGGRIGRSAVTEGALVSNGQSTALAEIQQLDPIYVDVTQPASELLRLRRELASG
                     QLEKVGANAAKVSLTLPDGSAYAHDGRLEFSEVSVDQGTGSVTLRAVFPNPEQVLLPG
                     MFVRAQLQAGSKSAAILAPQQGVTRNAKGEATAMVVNAENKVEVRVLKAERTAGSAWL
                     VSEGLKAGDRLITEGLQFVKPGVEVQVQPASNVPALAAPATATAAPTEPKPAQQPAQS
                     R"
     gene            1563..2201
                     /locus_tag="VIO83_03725"
     CDS             1563..2201
                     /locus_tag="VIO83_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY8330419.1"
                     /translation="MARRTKEDAEATRQQILDSAEHVFAAKGVAHTTMADIAADAGVS
                     RGALYWHFTSKTDIFHAMFIRQHDANKVVCAAARNPEEADPLAQMRSILSHFLRKVVD
                     DPQQRRVSEILHHKCELAGEQADLRQHLQATGDEIDQDIALSLRNAVQRGQLPADLDL
                     RRAAIAMHAYIHGLINNWLLHPDSYPLDREAEALVDALLDMLRFSPALRHPA"
     gene            complement(2242..3597)
                     /locus_tag="VIO83_03730"
     CDS             complement(2242..3597)
                     /locus_tag="VIO83_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY8330420.1"
                     /translation="MTLATDLLHTLQGTPAGLTLSELLEQHPDVVRRTAQRWLARLIE
                     AEQVAAEGNGRARRYRALTGVAPSPEGGDAFPSWIPLSADSRDILAYVDQPLAARRPV
                     GYQREFLEAYEPNRTWYLPEPLRRQLRRMGETGQGAAPAGTYGRAMLGRLLIDLSWAS
                     SHLEGNTYSRLDTVELIEHGQAAQGKAALETQMILNHKSAIELLVENADTVGFNRFTL
                     LNLHSALAENLLPNPADEGRVRQHAVEIGNSVYRPLSAPQQIDELLDQLLEKASRIAD
                     PFEQSFFVMVHLPYLQPFADINKRTSRLAANLPLFRANLCPLTFIGVPEQAYSRAVIG
                     LYELTRVELLRDLYVWAYERSTQEYLALRQTLAEPDPLRLAWRGLIRTSIREVVTQVE
                     ADPLLLVRRQVEREVPTAEREAVQALIVEELRRLHEGVLARYGLRPSEFAAWRAAQER
                     R"
     gene            complement(3734..4375)
                     /locus_tag="VIO83_03735"
     CDS             complement(3734..4375)
                     /locus_tag="VIO83_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY8330421.1"
                     /translation="MKLNNLASLDPAITSSGRKGLDGASKLDREIWAQFHADWEGLAL
                     ECAGLLAALRSEHNMPADDLDGDEVGDGLALADFTDATRQVLVEQRIKQQFFRRAVLG
                     SYRGRCCMSGLAEPRLLLASHIVPWRADKANRLNPANGLCLSALHDRAFDQGLITLED
                     DFTITLSEQLRQRDEPFVREVLRPLAGRQIELPERFMPSVEFVAWHRQQVFVG"
     gene            complement(4558..7866)
                     /locus_tag="VIO83_03740"
     CDS             complement(4558..7866)
                     /locus_tag="VIO83_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HEY8330422.1"
                     /translation="MNDKWEPCEDNRGGGAVAPARLVTGGEADHFLPHLCAAINAASE
                     IDMAVAFVKSTGLRLLLPDLLGALTRSDEAAAALQPARLRIITSDYLDITDPDALRRL
                     MLLQEQGAEVRVYESAGSSFHMKAYLFAAVDQHGQLRGQAFIGSSNISRQALQDGLEW
                     NYRIDYPGDAGFLEARLRFDQLFAYPRAKPLSDAWIDAYEARRIPPPRAIAPGSQEQE
                     LPPQPSAIQVEALQALLHSRAEGYRRGLVVLATGLGKTWLAAFDAEQFGARRVLFVAH
                     REEILNQAAETFLRIRPTARVGFYRGQQRDCLVDVLCASVQTLGRVAHLERFAPQHFD
                     YVVIDEFHHAAAPTYHRLLTHFAPSFLLGLTATPDRTDQSDILSLCDDNLVFTRDLFA
                     GIHSQLLAPFHYYGVFDDSVDYAEIPWRNGRFDPTQLSSKLATLSRARHALKTWRSHA
                     QSKTLAFCVSIRHAEFMAEYFQQQGVRAAAVYATSPLSRGEALEQLANGRLQAIFSVD
                     LFNEGVDLPAIDTVMLLRPTESKILFLQQLGRGLRKAPGKDQLVVLDFIGNHQSFLHK
                     PQALMGHSMNHRQLADYARAAEEHRLELPDGCFINYDLQLIDFLKALDGEGVQKDYQA
                     LREGLGRRPTLSEFYRFGASLREMRQQFGDWFSLVEAIGDLTDAEREVLSAQHALLVE
                     VEVRKIDDSLTMILLEAFQELDGWRVAPSLSALAERGWQTLQRRRALQADLPAALRQG
                     SSGLDAAWQRHWMGNPVAIWTGSQRKPGERVFFRIDGETLRPTQAVAEAHRETFAEWL
                     QELVDYRLAAYKTLPLAPPPPSRLGSELPFFPNLKIACGHFKTGTADCEEYRRLGEGH
                     GRLDPSRHFIARASGNSMNGGKQPIHDGDYLLLEQISPSSAGAITGSTMAIERQDGAG
                     DNQYLLRVILKERDGSYVLRANNPDFADIPVTDELQEQLRTFARLRAVLDPLEMAVGQ
                     RLMREEIPPLFGVEFNTGSWNVGHVLLAEAKSHILLVTLNKQGKAEEHRYLDHWIDER
                     TFHWQSQNSTTPESRRGREIIEHEKLGLSIHLFVREDRKQAGKAAPFTYHGPVRYRSH
                     SGSAPMSVVFELPN"
     gene            8148..8498
                     /locus_tag="VIO83_03745"
     CDS             8148..8498
                     /locus_tag="VIO83_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009042617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HEY8330423.1"
                     /translation="MSDRTGSSASYRQRESGYRDKALKMYPWVCGRCAREFAYSNLSQ
                     LTVHHIDHNHDNNPADGSNWELLCLYCHDHEHSKYSIYNGENGSAVIPGEKSPQVKHN
                     PFADLKALLDKQQK"
     gene            8583..9263
                     /locus_tag="VIO83_03750"
     CDS             8583..9263
                     /locus_tag="VIO83_03750"
                     /inference="COORDINATES: protein motif:HMM:NF007704.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HEY8330424.1"
                     /translation="MQEHEKPLDEAEIDELNELLFSYSEKAEQEHGKEGEEVDCIFSI
                     SELDGFLTAVLTGLNPISPSEWLPALWRGKLPKFESEAELQKLTDLLMRHMNALANSL
                     FEDPDSFAPIFEIGEDEDDVLVDEWCYGYMRGVELRNNDWQALRDQQPELLLPFMLLS
                     GAYSLEDGEEVSDDDIAEMSDNLPDLVFAVRDFWLAEAEKPKAPKTGRNDPCPCGSGK
                     KFKQCCLH"
     gene            9459..9830
                     /locus_tag="VIO83_03755"
     CDS             9459..9830
                     /locus_tag="VIO83_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019649736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein,
                     MvaT/MvaU family"
                     /protein_id="HEY8330425.1"
                     /translation="MSKLLEFRKAEQALREQLARLESLKHDTELKREMEFEDKLKELL
                     QEYGFKLQNIVDILQPQPLHIAELDLLEQDSGRRVRKTQIWTNPITGEVLETKSGNNK
                     TLKAWKAEHGEKFVATWLQKD"
     gene            10054..>10396
                     /locus_tag="VIO83_03760"
     CDS             10054..>10396
                     /locus_tag="VIO83_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY8330426.1"
                     /translation="MVLPLALLAFLAWRHWQGPLLPGYRVEARPLVQRVVASGEVSSQ
                     SLARIGSELTGVIKARHVREGDAVKPGDLLLELDDAEQQAKLREAEAALRQLIDSERP
                     QARAALREAESL"
BASE COUNT         1963 a         3529 c         3172 g         1732 t
ORIGIN      
        1 atgcccaccg ccggcggcgc gatgctcggg tactggttga tcggcagctt gaggatcgac
       61 agcccgccgg ccagcatgat caccagggcg atcacccagg cgaagatcgg ccggtcgata
      121 aagaatctag acatcagcga atccccttaa cggctctgcg ccggctgctg ggcaggcttc
      181 ggctcggtcg gcgcggccgt ggccgtggcc ggcgctgcca gcgccggtac gttgctggcc
      241 ggctgcacct ggacctcgac acccggcttg acgaactgca gaccctcggt gatcagccgg
      301 tcaccggcct tgagtccctc gctgaccagc caggcactgc cggcggtacg ctcggccttg
      361 aggacgcgca cttcgacctt gttctcggca ttgaccacca tcgccgtggc ctcgcccttg
      421 gcgttgcgcg tcaccccctg ctgcggcgcg aggatcgccg cgctcttgct gccggcctgc
      481 aactgcgcgc ggacgaacat gcccggcagc agcacctgct cagggttggg gaataccgcg
      541 cgcagcgtca ccgagccggt gccctggtcc accgacacct cggagaactc cagacgaccg
      601 tcatgggcat aggcgctgcc gtccggcagg gtcaggctca ccttggccgc attggcgccg
      661 accttttcca gctgcccgct ggccagctcg cgacgcaggc gcagcagctc gctggccggc
      721 tgggtcacgt cgacgtagat cgggtcgagc tgctggattt ccgccagcgc cgtactctgc
      781 ccgttgctca ccagcgcgcc ttcggtgacg gccgagcggc cgatgcgccc gccgatcggc
      841 gccagcactt tggtgtagcg cacatcgatg cgcgcctttt ccagcgccgc ttcggcctgc
      901 agctgggcgg cccgcgcctc gtcgtactgc tggcgactga ccgcctgatc ggccaccagc
      961 acctggtagc gctcggccag cgagcggctc gacagcacac tggcctcggc actcttcagc
     1021 gtcgcctcat aggtcgccgg atcgatctgg tacagctgct gcccggcctt gacctcgctg
     1081 ccctccttga acaggcgctt gtgcacgatg ccatcgacct gcggacgcac ctcggccacc
     1141 cggtaagcgg cggtgcgccc gggcacctcg gtggtcaggg tgaacggctg cgtctcgagg
     1201 gtcaccacgc ccacctgggg agccgggcgg gcctgcggag cggccgcctc tttctggcaa
     1261 ccagccaaca gcagaagcga cagcagggca gccgtgccca gcgtcgcgcg tgtggatacg
     1321 ctattcatcg gaaagcctcg aattcgtgtg cacgggcaag gggagaagta tgggggagat
     1381 gaaatctccg atggcgccca atatacttac atccgcgatt gtttgtaaga tcgcaactgt
     1441 tacaattttc cagtagcgcg acaaaaccac ggatcgtccg cacccggcca accctgcccc
     1501 actggcgcac gcaggtccgg acgccagcga cgaatctgcc acgaccggct cagagaaccc
     1561 ccatggcccg cagaacaaaa gaagacgccg aagccactcg ccagcagatc ctcgacagcg
     1621 ccgaacacgt gttcgccgcc aagggcgtgg cccacaccac catggccgac atcgccgccg
     1681 acgccggcgt ttcgcgcggc gccctgtact ggcacttcac cagcaagacc gacatcttcc
     1741 acgccatgtt catccgccag cacgacgcca acaaggtcgt ctgcgccgct gcccgcaacc
     1801 ccgaggaagc tgacccgctg gcgcagatgc gcagcatcct cagccacttc ctgcgcaagg
     1861 tggtggacga tccgcagcag cgccgggtca gcgaaatcct ccaccataag tgcgagctgg
     1921 ccggcgagca ggccgacctg cgccagcacc tgcaggccac cggcgacgag atcgaccagg
     1981 acatcgccct gtccctgcgc aacgccgtgc agcgcggcca gctgcccgcc gacctcgacc
     2041 tgcgccgcgc cgccatcgcc atgcatgcct acatccacgg cctgatcaac aactggctgc
     2101 tgcaccccga cagctacccc ctcgaccgcg aagcggaagc cttggtggat gccctgctcg
     2161 acatgctcag gttcagcccg gcgctgcgcc acccggcgta aggcgcccca ccccgcgttc
     2221 gcgatgcggg gtgcagacgc ctcagcgccg ctcctgagct gcccgccagg ccgcgaactc
     2281 cgagggtcgc agaccgtaac gcgccagcac gccctcgtgc agccgacgca actcctcgac
     2341 gatcagcgcc tgcaccgcct cgcgctcggc tgtcggcacc tcccgctcga cctgccgacg
     2401 caccagcagc aacggatctg cctccacctg cgtcaccacc tcgcgaatgc tcgtgcggat
     2461 cagcccgcgc caggccagac gcagcggatc gggctcggcc agcgtctgcc gcaacgccag
     2521 atactcctgg gtcgagcgct cgtaggccca gacatacaga tcgcgcagca actccacccg
     2581 cgtcagctcg taaaggccaa tgactgcccg gctgtaggcc tgttccggca ccccgatgaa
     2641 ggtcagcgga caaaggttgg cgcgaaacag cggcaggttg gccgccaggc gcgaagtgcg
     2701 cttgttgatg tcggcgaacg gctgcagata gggcagatgc accatcacga agaacgactg
     2761 ctcgaacggg tcggcgatcc ggctcgcctt ctccaacagc tgatccagca gctcatcgat
     2821 ctgctgaggt gcggacaatg gtcgatagac gctattgccg atctccaccg catgctgacg
     2881 cacccgcccc tcgtccgccg ggttgggcag caggttctcc gccagtgcgc tgtgcagatt
     2941 gagcagggtg aaacggttga agcccaccgt atcggcattc tccaccagca gctcgatggc
     3001 gcttttgtgg ttcaggatca tctgcgtttc cagggcagcc ttgccttgtg ccgcctggcc
     3061 atgctcgatc agctccacgg tatccagccg cgaataggtg ttgccctcca ggtgactgga
     3121 cgcccaggac aggtcgatca gcagccggcc aagcatcgcc ctcccatagg tgccggctgg
     3181 cgccgcgccc tgccccgtct cgcccatccg ccgcaactgg cggcgcaggg gttcgggcag
     3241 ataccaggtg cggttcggct cataggcctc gaggaattcg cgctgataac ccaccggccg
     3301 ccgcgccgcc agtggttgat caacataggc aagaatgtcg cgactgtcgg cggacagcgg
     3361 aatccacgac ggaaaggcat cgcccccctc tggggacggc gccacccctg tcagcgctcg
     3421 gtagcggcga gcgcgaccat tgccttctgc ggcgacttgt tccgcctcga tcaaccgggc
     3481 aagccagcgc tgcgccgtac gcctcactac atcgggatgc tgctccagca actcgctcaa
     3541 cgtcaggccc gcaggagtac cctgcagggt gtgcagcaga tcagtggcaa gcgtcatggc
     3601 agcctggctc cccggcagtg tcgcgattca tcaaaccgat gacgcgataa tgacgcgaaa
     3661 cacccaatcg cgtcacccga agtgtcgcga ttcccgacac ggatgacgcg attcccagca
     3721 accagtacct cactcaacca acaaacacct gctgccgatg ccaggcgacg aactcgacgc
     3781 tgggcatgaa acgctcgggc agctcgatct gccggccggc cagcgggcgg agcacctcgc
     3841 gcacgaaggg ttcgtcgcgc tggcgcaatt gttcggagag cgtgatggtg aagtcgtcct
     3901 caagggtaat cagcccctga tcgaaggcgc ggtcgtgcaa cgccgacagg cacaggccat
     3961 tggccggatt caagcggttc gccttgtccg cacgccaggg cacgatatgg ctggccagca
     4021 gcaggcgcgg ctcggccagc ccggacatgc agcagcgccc gcgataacta ccgagcaccg
     4081 cgcggcggaa gaactgctgc ttgatgcgct gctcgaccag cacttggcgg gtggcgtcgg
     4141 tgaagtcggc cagcgccagc ccgtcgccga cctcgtcccc atccaggtcg tcagccggca
     4201 tgttgtgttc gctgcgcaag gccgccagca gaccggcgca ctccagcgcc agcccttccc
     4261 agtcggcatg gaactgcgcc cagatttccc gatccagctt ggacgcgcca tccagcccct
     4321 tgcgtccgct ggaggtaatc gccggatcca gactggccaa gttattcaac ttcattgcca
     4381 ctgctccagg ctttcgacca atcaacccag ccagttcgat aacttgcgga gtccgctgat
     4441 ccaaacggcc aaagggcagt tggcaataca gatgcagcgc cagcttgacc tgatccgtgg
     4501 tccataaatc cttgcgcata gtgccgcctc cctgctgatt gcgccgactc acttggttca
     4561 gttgggcaat tcgaatacca cgctcatcgg cgcgctgccg ctatgactgc ggtagcgtac
     4621 cgggccgtga taggtgaaag gcgcggcctt gccggcttgc ttgcgatcct ctcggacaaa
     4681 caagtgaatg ctcagcccga gcttttcatg ctcgatgatc tcccgaccac gtctgctttc
     4741 cggagtagtc gaattctggc tttgccagtg gaaggtgcgc tcgtcgatcc aatgatccag
     4801 ataacggtgc tcttcggcct tgccctgctt attcagggtc accagcaata tgtgggattt
     4861 agcctcagcc aggagcacgt gcccgacatt ccaactgccg gtattgaact ccacgccgaa
     4921 cagcggcgga atctcctcgc gcatcagccg ctggcccacc gccatttcca ggggatcgag
     4981 cactgccctg aggcgggcga aggtgcgcag ctgttcctgc aactcatcgg tgactgggat
     5041 atcggcgaaa tcggggttgt tggcgcgcag cacatagctg ccgtcgcgct ccttgaggat
     5101 gacccgcagc aggtattggt tgtcgccagc accgtcctgg cgctcgatgg ccatggtcga
     5161 gccggtgatg gcacccgcgc tgctcggact gatctgctcc agcaacaggt agtcgccatc
     5221 atggatcggt tgcttgccgc cattcatcga gttacccgag gcgcgggcga taaagtggcg
     5281 ggacggatcg agacggccat ggccttcacc gagtcgccga tactcctcgc aatcggccgt
     5341 accggtcttg aagtggccgc aggcgatttt caggttgggg aagaacggca gttcgctgcc
     5401 tagacgacta ggcggtggag gggcaagcgg gagggttttg taggccgcca gccggtagtc
     5461 caccagctcc tgcagccact cggcaaacgt ctcgcggtgc gcctcggcaa ccgcctgcgt
     5521 ggggcgcagc gtttcgccgt cgatgcggaa aaacacccgc tcaccgggtt tgcgctggct
     5581 accggtccaa atcgcaacgg gattgcccat ccagtgacgc tgccatgccg catccagacc
     5641 gctactgccc tggcgcaacg ctgcaggcag atcggcctgc agcgcgcggc ggcgctgcag
     5701 agtctgccag ccgcgttcgg ccaaggcgct cagggacggc gccacgcgcc aaccgtccag
     5761 ttcctgaaac gcctccagca ggatcatcgt caggctgtcg tcgatcttgc gcacctcgac
     5821 ttctaccagc agcgcatgct gggcgctcaa tacctcgcgc tcggcgtcag tcagatcgcc
     5881 tattgcctcg accagcgaaa accaatcacc gaactgctgg cgcatctcgc gcagactggc
     5941 gccgaagcga tagaactcgc tcaaggttgg ccgccgcccc aggccttcgc gcagcgcctg
     6001 ataatccttc tgcacgccct cgccatccag cgccttgaga aagtcgatca gttgcaggtc
     6061 gtagttgatg aaacagccgt ccggcagttc caggcgatgc tcctcggcag cacgcgcata
     6121 gtcggccagt tgccgatggt tcatgctgtg gcccatcaat gcctgcggct tgtgcaggaa
     6181 actctggtgg ttgccgataa agtcgagcac caccaactgg tccttgcccg gcgccttgcg
     6241 cagaccgcga cccagctgct gcaggaaaag gattttcgac tcggttgggc gcagcagcat
     6301 caccgtgtcg atggccggca gatcgacacc ttcgttgaac aggtccaccg agaagatcgc
     6361 ctgtagccga ccgttcgcca attgctccaa cgcctcgccg cgcgacaggg gcgaagtggc
     6421 atacaccgcc gcggcccgca caccctgctg ctggaaatac tcggccatga actcggcgtg
     6481 gcgaatcgat acacagaacg ccagcgtctt gctctgtgcg tggctgcgcc acgtcttcaa
     6541 ggcgtgccgg gcgcggctca gggtcgccaa tttgctgctt agctgcgtgg gatcgaaacg
     6601 gccattgcgc caggggattt ccgcgtaatc cacgctgtca tcgaacaccc cgtaatagtg
     6661 gaagggcgcg agcaactggc tgtggatgcc ggcgaacaag tcgcgggtaa acaccagatt
     6721 gtcgtcgcac agcgacagaa tatccgactg gtcggtgcga tccggcgtgg cggtcaggcc
     6781 gagcaggaag cttggcgcaa agtgggtgag caggcggtga taggtcggcg cggcagcatg
     6841 gtggaattcg tcgatcacca cgtaatcgaa gtgctgcggt gcaaaacgct ccagatgggc
     6901 gacgcgaccc aaggtctgca ccgaagcgca cagcacatcc accaggcagt cgcgctgctg
     6961 gccccgatag aaaccaaccc gcgcggtcgg tcgaatgcgc aggaaggttt ccgccgcttg
     7021 attgagaatt tcctcgcgat gggcgacgaa cagcacccgc cgcgcaccga actgctcggc
     7081 atcgaaggcc gccagccagg tcttgcccag gccggtagcg aggaccacca ggccgcggcg
     7141 gtatccctcg gcgcggctgt gcagcaaggc ctgcagcgcc tcgacctgaa tcgcgctcgg
     7201 ctgcggtggc agctcctgct cctgactccc cggcgcaatc gcccgcggcg gcgggatgcg
     7261 ccgggcttcg taggcgtcga tccaggcatc tgaaagcggc ttggcgcgcg gataggcgaa
     7321 caactggtcg aagcgcagcc gcgcctcgag aaaacccgca tcgcccggat agtcgatgcg
     7381 gtagttccac tccaggccat cctgcagcgc ctggcggctg atattgctgg agccgataaa
     7441 ggcttggccg cgtaactggc catgctgatc caccgcggca aacagataag ccttcatgtg
     7501 aaagctgctg ccggcgcttt catagacgcg tacctcggcg ccctgctctt gcagcagcat
     7561 cagccggcgc agcgcgtcgg gatcggtgat gtcgaggtaa tcgctggtga tgatgcgcag
     7621 ccgcgccggc tgcagcgccg ccgcagcctc gtcactgcgg gtcaacgcgc ccagcaagtc
     7681 aggcaacagc aggcgcagac cggtgctttt gacgaaggcc accgccatgt cgatctcgct
     7741 ggcggcgttg atcgccgcgc acagatgcgg caggaaatga tcggcttcgc cgccagtgac
     7801 caggcgtgcc ggtgcgacgg cgccaccacc gcggttatct tcgcaaggct cccacttatc
     7861 gttcacccgt cacgtccttg ccctgtttca cgtccatcga gcaaccatgc tatcaccccg
     7921 ccagcccctg ttcgccgcta ccaccgcccc cagcgcctcg cgccagccga tccatccggc
     7981 catccaagcg cctgaaagcc tgacggtagt gagccgctga ctgccgcccg gacactgcag
     8041 tccggcgcgc cgctcttgcc tgcccggcga acatgcccaa cactcgcagc aagcggtggc
     8101 gcccaggtgt gccacctcgt tcaatccagg aaaatgtgag cactaccatg tcggacagaa
     8161 caggcagcag cgccagctac aggcagcgcg aatcggggta ccgggacaag gccctgaaga
     8221 tgtatccgtg ggtctgcggc cgctgcgcgc gggaattcgc ctattccaac ctgagccagc
     8281 tgacggtcca ccatatcgac cacaatcacg acaacaatcc ggccgacggc agcaactggg
     8341 agctgctctg cctgtattgt cacgaccacg aacactccaa gtattcgatc tacaacggcg
     8401 agaacggcag tgcggtgatt cccggcgaga agtcgccgca ggttaagcac aatccgttcg
     8461 ccgacctgaa ggcgctgctg gacaagcagc agaaataatt ccggccgggg cgcagggcgc
     8521 gcacggcgcc ccgcatggcc tctgttaata ttcgccccat tcccaactcc aggaaaattc
     8581 tcatgcaaga acacgaaaag cccctcgacg aggcggaaat cgacgaactg aacgaactgc
     8641 tgttcagcta ctcggaaaag gccgagcagg aacatggcaa ggaaggcgag gaggtggatt
     8701 gcatattcag catctccgag ctggacggtt tcctcaccgc cgtgctgacc ggtctcaatc
     8761 cgatttcgcc gtcggaatgg ttgccggcgc tgtggcgcgg caagctgccg aagttcgaat
     8821 cggaagccga gctgcaaaaa ctcaccgacc tgctgatgcg ccacatgaat gccctggcca
     8881 acagcctgtt cgaggatccg gacagcttcg cgccgatctt cgagatcggc gaagacgaag
     8941 acgacgtgct ggtcgacgag tggtgctacg gctacatgcg cggcgtcgag ctgcgcaaca
     9001 acgactggca ggccctgcgc gaccagcagc cggaactgct gctgccgttc atgctgctga
     9061 gcggcgccta ctcgctggaa gacggcgagg aagtgtccga tgacgatatc gccgagatga
     9121 gcgacaacct gccggacctg gtgtttgccg tgcgcgattt ctggctggcc gaggcggaaa
     9181 agcccaaggc gccgaagacc ggccgcaacg atccgtgccc gtgcggcagc ggcaagaagt
     9241 tcaagcaatg ctgcctgcac tgaggcgcca ctgaaactct ccaccccgct gcccgccgtc
     9301 gcttgcacta ctctttgcac tattgacggt gggcagccgc cccgataaaa agcacacgct
     9361 tatccgcagc gaaaacaatt ggatgcaggg attgcacggc tttcgcgcat gcggactttc
     9421 ttattgcaga cccttcttct acatcctgga gacacttgat gtccaaactg ctggaatttc
     9481 gcaaggccga acaagccctc agggagcaac tcgcgcgact ggaatcgctc aagcacgaca
     9541 ccgaactgaa gcgcgaaatg gagttcgagg acaagctcaa ggaattgctg caggaatacg
     9601 gtttcaaact gcagaatatc gtcgatattc tccagccgca gccgctgcat atcgcggaac
     9661 tcgacctcct ggaacaggac agcggtcgcc gcgtacgcaa gacccagatc tggacgaatc
     9721 cgatcaccgg cgaagtactg gaaaccaaga gcggcaacaa caagaccctc aaggcctgga
     9781 aggccgaaca cggcgagaag ttcgtcgcca cctggctgca gaaggactga agtccgccag
     9841 ccggccctga tcgctcccat attcctctat gggagcggcc aatgccgcgt ggctcacgcc
     9901 cagagcgtgg cacgcgcatc cccgcccctg cgacgccccg gcctgtagcc ggccgaccga
     9961 ttttctcgca cactgccggc catccatacc ggtcctgtca gggagtgata cgtgtcgcct
    10021 gccgccttcc gccgtcgccc tgccctcctg ctgatggtcc tgccgctcgc gctgctggcc
    10081 ttcctcgcct ggcgccactg gcaaggcccg ctattgcccg gctatcgggt cgaggcgcgg
    10141 ccactggtgc agcgggtggt ggccagcggc gaggtcagca gccagtcgct ggcgcgcatc
    10201 ggcagcgagc tgaccggggt gatcaaggcc cggcatgtgc gcgaaggcga tgcggtaaag
    10261 ccgggcgacc tgctgctgga gctcgacgac gccgagcagc aggccaagct gcgcgaggcc
    10321 gaggcggcgc tgcgccagct gatcgactcc gaacgcccgc aggcccgcgc ggcgttgcgc
    10381 gaggcagaga gcctgc
//
LOCUS       DATCUN010000099        10506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_192123, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000099 DATCUN010000000
VERSION     DATCUN010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10506
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192123"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /gene="arfA"
                     /locus_tag="VIO83_03765"
     CDS             <1..152
                     /gene="arfA"
                     /locus_tag="VIO83_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015096971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072344 - rescue of stalled ribosome
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alternative ribosome rescue factor ArfA"
                     /protein_id="HEY8330427.1"
                     /translation="NKAKSLVAQPLFRCRQERPAKGKGSYQREAFRFTDREASSFPGQ
                     EKATA"
     gene            complement(186..1076)
                     /locus_tag="VIO83_03770"
     CDS             complement(186..1076)
                     /locus_tag="VIO83_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HEY8330428.1"
                     /translation="MKIPKRLEPLAEDGLIDEVLRPLMSGKEAAVYVVRCGTELRCAK
                     VYKEANKRGFRQAAEYQEGRKVRNSRDARAMAKGSKHGRKEQEEAWQNAEVAALYRLA
                     SAGVRVPKPYDFLDGVLLMEMVAGADGDAAPRLNDVDLTPEQAREYHAFMIRQVVLML
                     CAGLVHGDLSEFNVLLGPDGPVVIDLPQAVDAAGNNHAFKMLERDVANMNAYFGHFAP
                     ELLTRKHAKEMWALFEAGKLTAESELTGEFDEPEEEADVDAVMREINAALADEERRQA
                     ALAAEDAPPQREEPPPPWLG"
     gene            1524..2432
                     /locus_tag="VIO83_03775"
     CDS             1524..2432
                     /locus_tag="VIO83_03775"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8330429.1"
                     /translation="MRIAPRLLSSAIAIALLSSPAFAEELSGTLKKIKESGTVTLGHR
                     DSSIPFSYLGTEPGQPIGYAHDLQLKVVEALKQKLGIPELKVRYNLVTSQTRIPLVQN
                     GTVDLECGSTTNNLERQKQVDFSVGIFEVGTRLLTKKTSGIADFADLKGKNVVTTAGT
                     TSERLLKSMNADQQMGMNIISAKDHGESFLMLESGRAVAFMMDDVLLYGEMAKARKPD
                     EWTVVGTPQSYEIYGCMLRKGDPAFKQVVDDAITATFKSGEINAIYDKWFLQPVPPKS
                     LNLNFPMSEQFKKLVVEPTDKSAEQL"
     gene            2510..3256
                     /locus_tag="VIO83_03780"
     CDS             2510..3256
                     /locus_tag="VIO83_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY8330430.1"
                     /translation="MNYNWDWGVFLKSTGIGDEIYLDWFITGFGWTLAVALVAWTIAL
                     LLGSLLGVLRTVPNRWLSGIATVYVETFRNVPLLVQLFLWYFLVPDLLPTPLETWFKQ
                     DLSPATSAYLSVIVCLGLFTSARVCEQVRTGIQALPKGQAAAACALGFTLPQIYRQVL
                     LPQAFRIIIPPLTSEFLNIFKNSAVASLIGLMELLAQTKQTAEFSANLFEAFTLATLI
                     YFTLNMSLMLFMRFVEKKVAVPGQISAGGK"
     gene            3253..3921
                     /locus_tag="VIO83_03785"
     CDS             3253..3921
                     /locus_tag="VIO83_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY8330431.1"
                     /translation="MMDFSAIPAALPGLWDGMQLTLQLLVLGVFGGVVLGTLLAMMRL
                     SHNKLLSGLATAYVNYFRSIPLLLVITWFYFAVPFILRWITGEDTPVGAFTSCLVAFV
                     MFEAAYFCEIVRAGIQAVPKGQMSAAQALGMNYAQTMRLIILPQAFRKMTPLLLQQSI
                     ILFQDTSLVYTVGLMDFLNAARSRGDILGQPHEFLIFAGLVYFIISFAASQLVKRLQK
                     RLTV"
     gene            3918..4652
                     /locus_tag="VIO83_03790"
     CDS             3918..4652
                     /locus_tag="VIO83_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY8330432.1"
                     /translation="MISINNVSKWYGDFQVLTDCTTQVSKGEVVVVCGPSGSGKSTLI
                     KCVNALEPFQKGDIVVDGTSIANPQTNLSKLRSRVGMVFQHFELFPHLSITENLTLAQ
                     IKVLGRSKEEATAKGLKLLDRVGLKAHAHKYPGQLSGGQQQRVAIARALAMAPVVMLF
                     DEPTSALDPEMVNEVLDVMVQLAQEGMTMMCVTHEMGFARKVANRVIFMDRGQIVEDC
                     AKEEFFGDVNARSERAQQFLAKILQH"
     gene            4745..6643
                     /locus_tag="VIO83_03795"
     CDS             4745..6643
                     /locus_tag="VIO83_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8330433.1"
                     /translation="MQSPHPSPCALPPSLTVNPRLLPPLYLPLLAILLMFGCGYLAYH
                     LSEQAGLRALADNGERQLELHARAVESAISTHTYLPSLLELEDGVSRLLLAPSEARRT
                     RVNQYLEGLNQRSGSRAIYLLDRSGRVLATSNWRDADSFQGEDLSFRAYFQDALQGRP
                     GRFYGVGSTTGEPGYYLAHGLRHDGQIIGVAVVKIRLDPLEQRWQRAQLEAFVSDDNG
                     IVILSSDPARRFKSVQPLDAERRAQLAASLQYRWTRFEELQPLGRQRLSADTELLTLP
                     GANGARPLAYLAQTLSLNDPPWQFTLLSPVQEVRRAATSHGLLAAVAFALLAFLLIAW
                     NERRKTLATRLAAREALEQANSQLERRIAERTEHLRASNEHLKAQIRERRQAEESLRR
                     TQDDLVQAGKLAVIGQMSTCIAHELNQPLAALRTLSGNTVRFLARGDNATASSNLQTI
                     NELVDHMGRITASLRAFARRSDDRGEAELGKAVDAALFLLHPRLERTPVTLHRACAEA
                     RLAIDQTRLEQILVNLIVNAIDAMSAQSDRQLWLDGQREDGHYCLAVRDNGPGIPPAL
                     RRQLFEPFFTTKPGEQGLGLGLTLSASLAAAAGGSLTAQHPEAGGTLFELKLPLLPPS
                     PAESPRDE"
     gene            6636..7961
                     /locus_tag="VIO83_03800"
     CDS             6636..7961
                     /locus_tag="VIO83_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8330434.1"
                     /translation="MNEPLTVLIVEDDPHVLLGCQQALALEDIPSEGVGSAEEALARI
                     GADFAGIVISDIRLPGMDGLTLLGQLKARDRSLPVVLITGHGDISMAVGAMRDGAYDF
                     MEKPFSPERLVDVARRALEQRSLAREVSALRRQLAGRQTLEERLIGRSPAMAQLRALI
                     ANVADTSANVLIEGETGTGKELVARCLHDFSRRQSRPFVALNCGGLPESLFESEIFGH
                     EAHAFTGAGKRRIGKIEHANGGTLFLDEIESMPLNLQIKLLRVLQEHSLERLGSNQSI
                     AVDCRVIAATKADLDELGRRGQFRSDLYYRLNVVTLELPPLRERREDIPLLFEHFLQQ
                     SSLRFDRPAPELDRQTLASLLAHDWPGNVRELRNVAERFALGLPVFKKGGASAESVGP
                     NFAEAVEAFEKRLINDALERHGGNLSQASQALGMAKTTLFDKVKKYRLQ"
     gene            8030..8335
                     /locus_tag="VIO83_03805"
     CDS             8030..8335
                     /locus_tag="VIO83_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011059051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEY8330435.1"
                     /translation="MNIDELTRRLHAIRDHNDWRRFHSPKNLAMAASVEMAELVEIFQ
                     WLSEDEARALPPAQLEHAGQEIGDVVLYLLLLCSELGLDLDNVVRAKLADTERRYKA"
     gene            8347..9114
                     /locus_tag="VIO83_03810"
     CDS             8347..9114
                     /locus_tag="VIO83_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008093119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8330436.1"
                     /translation="MQQPAPHDRHFDELATRFAEKIYGGAKGAIRLAGLQADLKEVLP
                     ERPLRVLDVGAGLGHMSLWLAGHGHQVTLAEPAAPMLDGARARFAEAGLQASFIQAPW
                     QELLGQFEQPFDLVLCHAVLEWLAEPPAILPVLHQLTAADGWLSLAFYNRDALIYRNL
                     LKGHFRKLRKERFAGEGQSLTPQRPLDPRELAGQLDDWFRVEQQSGVRVFHDYMPAEF
                     QARAELTDLLEMELAHRRHPAFAGLGRYLHWLCRPQP"
     gene            9186..9761
                     /locus_tag="VIO83_03815"
     CDS             9186..9761
                     /locus_tag="VIO83_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEY8330437.1"
                     /translation="MAVRAPTLLLLVLLSGCQSGNPYTASHLPYPPPPREAAALQVDP
                     GSYPPTPRDFAQYRNWQWAAAVDEPLAGIVSAELDQRGLRPATAQSPATLSVRAQQRS
                     VTRQQQVYDDPYFDAGYGHYSHHHGYWGGTGYPLVRTISYRVDEVQLELFDARSGERV
                     WSGSGEAESGRARDDSLRRAVRQALDGFPPP"
     gene            9835..10401
                     /locus_tag="VIO83_03820"
     CDS             9835..10401
                     /locus_tag="VIO83_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEY8330438.1"
                     /translation="MFRRLLLIATLLALAACQSAQLDSDYDPNRDFAALQTWSWQEPA
                     VQYRPDDPRLRSDLTEQRLRQAVAAQLEQRGLRQAAANAAADIRVQAFLMVENRQQQM
                     ATYYGSDMMSDRWGGHWASPGYTEVRTLDYQVGTLQLDFLDRDGKLVWRGSAAQIVPR
                     HAGSPQERAASIQQTVAKILAQYPPRGR"
     gene            10409..>10506
                     /locus_tag="VIO83_03825"
     CDS             10409..>10506
                     /locus_tag="VIO83_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY8330439.1"
                     /translation="MGQRILILVASGPSTPLRCAAPFHIATLLACM"
BASE COUNT         1736 a         3906 c         3280 g         1584 t
ORIGIN      
        1 cgaacaaggc caaatccctg gttgcacaac cgctgttccg ctgccgccag gaacgcccgg
       61 ccaagggcaa gggcagctac caacgcgaag ccttccggtt caccgaccgg gaggcttctt
      121 cctttccggg ccaggaaaaa gccaccgcct gagcgacgtc ccctccccgg ctcatggccc
      181 gctgattagc ccaaccacgg tggcggcggt tcctcgcgct gcggcggcgc atcttccgcc
      241 gccaatgccg cctggcggcg ctcttcgtcg gccagtgccg cgttgatctc gcgcatcacc
      301 gcgtcgacat ccgcctcttc ttccggctca tcgaactcgc cggtcagctc gctctccgcc
      361 gtcagcttgc cggcctcgaa cagcgcccac atttccttgg catgcttgcg cgtcagcaac
      421 tcgggcgcga agtggccgaa ataggcgttc atgttggcaa cgtcgcgctc gagcatcttg
      481 aacgcatgat tgttgccggc ggcatccacg gcctgcggca ggtcgatcac caccgggccg
      541 tccggaccga gcagcacgtt gaactccgag aggtcgccat gcaccaggcc ggcgcagagc
      601 atcaacacga cctggcggat catgaaggca tggtattcac gcgcctgctc gggcgtcagg
      661 tcgacatcgt tgaggcgtgg cgccgcatcg ccgtcggcac cggccaccat ctccatcagc
      721 agcacgccgt cgagaaagtc gtagggcttg ggcacgcgca cgccggcact ggccaggcgg
      781 tagagcgcgg ccacctcggc gttctgccag gcttcctcct gctccttgcg gccgtgcttg
      841 ctgcccttgg ccatggcccg cgcatcgcgg ctgttgcgga ccttgcggcc ctcctggtat
      901 tcggcggcct ggcggaagcc acgcttgttg gcctccttgt agaccttggc gcagcgcagc
      961 tcggtgccgc agcgcaccac gtacacggct gcttccttgc cgctcatcag cggacgcagc
     1021 acctcgtcga tcaggccatc ctcggccagc ggctcaagac gtttgggtat cttcatcggt
     1081 tttactgcag gtccttgcca tgaaacgggg ggaaaccggc gtgccgatac ctcggcaggc
     1141 gtggtcggca caaactagct gcacgttata cgccaaacac cccgaggccg cccagcaccg
     1201 ggcgaccgca gcccggccag gcgcgcacgc ccgcgacccc gcactggcgt ccggttttcc
     1261 gaacgcaatt gggccaggtg tccggccagc cggacgaaga gcgcccgaac ggccaccgca
     1321 ttgaaattaa aactctttat tttcaattac ttatgaatat aaccaggcca ggaaaaagct
     1381 ggcccgcatc ctgcacctag acttgtcata agcgcgatac gcctgccact cgagcctggt
     1441 gcccgcacgt caggtagccg ttcccacaat ggagcgagcg gtatcgctgt ttacgcaccc
     1501 aacaacaagt cgaggaaaca ctcatgcgta tcgccccccg tttgctgagc agtgccatcg
     1561 ccatcgccct gctgtccagc ccggcctttg ccgaagagct cagcggcacc ctgaagaaga
     1621 tcaaggagtc cggcaccgtc accctcggcc accgcgattc ctcgattccc ttctcctacc
     1681 tgggcacgga gccgggccag ccgatcggct acgcccacga cctgcagctc aaggtggtcg
     1741 aggcgctcaa gcagaagctc ggcatcccgg aactgaaggt gcgctacaac ctggtcacct
     1801 cgcagacccg catcccgctg gtgcagaacg gcaccgtcga cctcgagtgc ggctcgacca
     1861 ccaacaacct ggagcggcag aagcaggtgg atttctcggt gggcatcttc gaggtcggca
     1921 cccgcctgct gaccaagaag acctccggca tcgccgactt cgccgacctc aagggcaaga
     1981 acgtggtgac caccgccggc accacctccg agcgcctgct caagtcgatg aacgccgacc
     2041 agcagatggg catgaacatc atctcggcca aggaccacgg cgagtccttc ctgatgctcg
     2101 aatccggccg cgcggtggcc ttcatgatgg acgacgtgct gctctacggc gaaatggcca
     2161 aggcgcgcaa gccggacgag tggaccgtgg tcggcacgcc gcagtcctac gagatctacg
     2221 gctgcatgct gcgcaagggc gacccggcgt tcaagcaggt ggtcgatgac gccatcaccg
     2281 ccaccttcaa gtccggcgag atcaacgcca tctacgacaa gtggttcctc cagccggtgc
     2341 cgccgaagag cctcaacctg aacttcccga tgagcgagca gttcaagaag ctggtcgtcg
     2401 agccgaccga caagtcggcc gaacagctgt aagccgtgcc tgtccgcggg gcgtgcgcgc
     2461 cccgcgccac ggccagcctt gcgtcacata acgagaaggg gtaacccgca tgaactacaa
     2521 ctgggactgg ggcgtgttcc tcaagtccac cggcatcggc gacgagatct atctggactg
     2581 gttcatcacc ggtttcggct ggaccctcgc ggtcgccctg gtggcctgga ccatcgccct
     2641 gctgctcggc tcgctgctcg gcgtgctgcg taccgtaccg aaccgctggc tgtccggcat
     2701 cgccaccgtc tacgtggaaa ccttccgcaa cgtgccgctg ctggtgcagc tgttcctctg
     2761 gtacttcctg gtgcccgacc tgctgcccac gccgctggaa acctggttca agcaggacct
     2821 cagcccggcc acctcggcct acctgagcgt gatcgtctgc ctcggcctgt tcacctccgc
     2881 gcgggtctgc gagcaggtgc gcaccggcat ccaggcgctg cccaagggcc aggccgccgc
     2941 cgcctgcgcg ctgggcttca ccctgccgca gatctaccgc caggtactgc tgccgcaggc
     3001 cttccgcatc atcatcccgc cgctgacctc ggagtttctc aacatcttca agaactccgc
     3061 ggtggcctcg ctgatcggcc tgatggagtt gctggcgcag accaagcaga ccgcggagtt
     3121 ctccgccaac ctgttcgagg ccttcaccct ggccacgctg atctacttca ccctgaacat
     3181 gagcctgatg ctgttcatgc gcttcgtcga gaagaaggtc gcagtgccgg gccagatctc
     3241 cgcaggaggt aaatgatgga tttcagcgca attcccgcgg ccctgccggg actctgggac
     3301 ggcatgcagc tgaccctgca gctgctggtg ctgggcgtgt tcggcggcgt ggtgctgggc
     3361 accctgctgg cgatgatgcg cctgtcgcac aacaagctgc tgtccggcct ggccaccgcc
     3421 tacgtcaact acttccgctc catcccgctg ctgctggtga tcacctggtt ctacttcgcg
     3481 gtgccgttca tcctgcgctg gatcaccggc gaggacaccc cggtgggtgc cttcacctcg
     3541 tgcctggtgg ccttcgtgat gttcgaggcg gcgtacttct gcgagatcgt ccgcgccggc
     3601 atccaggcgg tgcccaaggg ccagatgagc gccgcccagg cgctggggat gaactacgcg
     3661 cagaccatgc gcctgatcat cctgccccag gcgttccgca agatgacccc gctgctgctg
     3721 cagcagagca tcatcctgtt ccaggacacc tcgctggtgt acacggtcgg tctgatggac
     3781 ttcctcaacg ccgcccgctc gcgcggcgac atcctcggcc agccccatga gttcctcatc
     3841 ttcgccggcc tggtgtactt catcatcagc ttcgctgcct cgcagctggt caagcgtctg
     3901 cagaaaaggt taaccgtatg atctcgatca acaacgtcag caagtggtac ggcgacttcc
     3961 aggtcctgac cgactgcacc acccaggtga gcaagggcga ggtggtggtg gtgtgcggtc
     4021 cgtccggctc gggcaagtcg accctgatca agtgcgtcaa cgccctggag ccgttccaga
     4081 aaggcgacat cgtggtcgac ggcacctcga tcgccaatcc gcagaccaac ctgtccaagc
     4141 tgcgctcgcg ggtcggcatg gtgttccagc acttcgaact gttcccgcac ctgtcgatca
     4201 ccgagaacct gaccctcgcg cagatcaagg tgctcggccg cagcaaggag gaagccaccg
     4261 ccaagggcct caagctgctc gatcgggtcg gcctcaaggc ccatgcgcac aagtatcccg
     4321 gccagctgtc cggcggccag cagcagcgcg tggcgatcgc ccgcgcgctg gccatggccc
     4381 cggtggtgat gctgttcgac gaaccgacct cggcgctcga cccggagatg gtcaacgagg
     4441 tgctcgacgt gatggtccag ctcgcccagg aaggcatgac catgatgtgc gtgacccacg
     4501 agatgggctt cgcgcgcaag gtggccaacc gggtgatctt catggaccgt gggcagatcg
     4561 tcgaggactg cgccaaggag gagttcttcg gcgacgtcaa cgcccgctcc gagcgcgccc
     4621 agcagttcct cgccaagatc ctccagcact gaaggcccgg gcggcggggt catcccgccg
     4681 cccggctccg gccacccaga aacggccagc ccggcgccac actggccgca cggacccgat
     4741 tgtgatgcaa tccccccatc ccagcccctg cgcgttgccg ccctcgctga ccgtgaaccc
     4801 gcgcctgctc ccgcccctgt acctgccgct gctggccatc ctgctgatgt tcggctgcgg
     4861 ctacctcgcc taccacctga gcgagcaggc cggcctgcgc gctctggcgg ataacggcga
     4921 gcgccagctg gagctgcacg cccgcgcggt ggaaagcgcg atcagcaccc acacctacct
     4981 gcccagtctg ctggagctgg aggatggtgt cagccgcctg ctgctggcgc cgagcgaggc
     5041 gaggcgcacc cgggtcaacc agtacctcga aggcctcaac cagcgcagcg gcagccgcgc
     5101 gatctacctg ctcgaccgca gcggccgggt gctggccacc agcaactggc gcgatgccga
     5161 cagtttccag ggcgaggacc tgtccttccg cgcctacttc caggacgccc tgcagggcag
     5221 gcccgggcgc ttctacggcg tcggcagcac caccggcgag cccggctact acctggccca
     5281 cggcctgcgc cacgatgggc agatcatcgg cgtggcagtg gtgaagatcc gcctcgaccc
     5341 gctggagcag cgctggcagc gcgcccagct ggaggccttc gtcagcgacg acaacggcat
     5401 cgtcatcctc tccagcgacc ccgcgcgccg cttcaagtcg gtgcagcccc tcgacgccga
     5461 gcgccgtgcg cagctggccg ccagcctgca ataccgctgg acccgcttcg aggagctgca
     5521 gccgctgggc cgccaacgcc tgtccgccga caccgaactg ctgaccctgc cgggcgccaa
     5581 cggcgcgcgg ccgctggcct acctggcgca gaccctgagc ctcaacgacc cgccgtggca
     5641 gttcaccctg ctctcgccgg tacaggaggt gcgccgcgcc gccaccagcc acggcctgct
     5701 cgccgcggtg gccttcgccc tgctggcctt cctgctgatc gcctggaacg agcgacgcaa
     5761 gacgctggcc acccgcctgg ccgcccgcga ggcgctggag caggccaaca gccagctgga
     5821 gcggcgcatc gccgagcgca ccgagcacct gcgcgccagc aacgagcacc tcaaggcgca
     5881 gatccgcgag cggcgccagg ccgaggaaag cctgcgccgg acccaggacg acctggtgca
     5941 ggccggcaag ctggcggtga tcggccagat gtccacctgc atcgcccacg aactcaacca
     6001 gccgctggcc gccctgcgca ccctgtccgg caacaccgtg cgcttcctcg cccgcggcga
     6061 caatgccacc gccagcagca acctgcagac catcaacgag ctggtcgacc acatgggccg
     6121 catcaccgcc agcctgcgcg ccttcgcccg gcgttccgac gaccgcggcg aagcggagct
     6181 gggcaaggcg gtggacgccg ccctgttcct cctccatccg cgcctggagc gcacgccagt
     6241 gaccctccac cgcgcctgtg ccgaggcccg cctggccatc gaccagaccc gcctggagca
     6301 gatcctggtc aacctgatcg tcaacgccat cgacgcgatg agcgcgcaga gcgaccgcca
     6361 gctgtggctg gacggccagc gcgaggacgg ccactactgc ctcgccgtgc gcgacaacgg
     6421 ccccggcatc ccgccggcgc tgcgccgcca gctgttcgag ccgttcttca ccaccaagcc
     6481 gggcgaacag ggcctcggcc tcggcctgac cctgtcggcc agcctggccg ccgccgccgg
     6541 cggcagcctg accgcccagc acccggaagc cggcggcacc ctgttcgagc tgaagctgcc
     6601 gctgctcccc ccttcccctg ccgagtcccc ccgcgatgaa tgagccactg accgtcctga
     6661 tcgtcgagga cgacccccac gtgctgctcg gctgccagca ggcgctggcc ctcgaggaca
     6721 tccccagcga gggcgtcggc agcgccgagg aagcgctggc gcggatcggc gcggacttcg
     6781 ccggcatcgt catcagcgac atccgcctgc ccggcatgga cggcctgacc ctgctcggcc
     6841 agctcaaggc gcgcgaccgc agcctgccgg tggtattgat caccggtcac ggcgacatct
     6901 ccatggcggt cggcgcgatg cgcgacggcg cctacgactt catggaaaag ccgttctccc
     6961 ccgagcggct ggtcgacgtg gcgcgccgcg ccctcgagca gcgcagcctg gcccgcgagg
     7021 tgtcggcgct gcgccgccag ctggccggcc gccagaccct ggaagaacgc ctgatcggcc
     7081 gctcgccggc catggcgcag ctgcgtgcgc tgatcgccaa cgtcgccgac acctcggcca
     7141 acgtgctgat cgagggcgag accggcaccg gcaaggagct ggtggcgcgc tgcctgcacg
     7201 acttcagccg gcgacagtcc cgcccgttcg tcgccctcaa ctgcggcggc ctgccggaaa
     7261 gcctgttcga gagcgagatc ttcggccacg aggcgcacgc cttcaccggc gccggcaagc
     7321 ggcgcatcgg caagatcgaa cacgccaatg gtggcaccct gttcctcgac gagatcgaga
     7381 gcatgccgct caacctgcag atcaagctgc tgcgcgtgct gcaggagcac agcctggagc
     7441 gcctcggctc caaccagagc atcgccgtgg actgtcgggt gatcgccgcc accaaggccg
     7501 acctcgacga gctgggccgc cgcgggcagt tccgcagcga cctgtactac cgcctcaacg
     7561 tggtcaccct cgagctgccg ccgctgcgcg agcgccgcga ggacatcccg ctgctgttcg
     7621 agcacttcct ccagcagtcc tcgctgcgct tcgaccgccc cgccccggag ctggaccgcc
     7681 agaccctggc cagcctgctg gcccacgact ggccgggcaa cgtgcgcgag ctgcgcaacg
     7741 tcgccgagcg cttcgccctc ggcctgccgg tattcaagaa gggcggcgcc agcgcggaaa
     7801 gcgtcgggcc gaacttcgcc gaggcggtgg aagccttcga gaaacgcctg atcaatgatg
     7861 ccctggagcg ccacggcggc aacctcagcc aggccagcca ggcgctgggc atggccaaga
     7921 ccaccctgtt cgacaaggtg aagaagtacc gcctgcagta gggtgcgccc tacccggact
     7981 tgcagcccgc gcgctcccgg ccgaagctat gcgccatcaa ggagcgccca tgaacatcga
     8041 tgaactgacc cgccgcctgc acgccatccg cgaccacaac gactggcggc gctttcacag
     8101 cccgaagaac ctggccatgg ccgccagcgt ggaaatggcc gagctggtgg aaatcttcca
     8161 gtggctgagc gaagacgagg cgcgcgccct gccgcccgca cagctggagc acgccggcca
     8221 ggagatcggc gacgtggtgc tctacctcct gctgctgtgc agcgagctgg gcctcgacct
     8281 cgacaacgtg gtgcgcgcca agctggccga caccgaacgg cgctacaagg catgagcggt
     8341 cacgacatgc agcagcccgc cccccacgac cgccatttcg acgaactggc cacgcgcttt
     8401 gccgagaaga tctacggcgg cgccaagggc gcgatccgcc tcgccgggct gcaggccgac
     8461 ctcaaggaag tgctgcccga gcgcccgctg cgggtgctcg acgtcggcgc cggcctcggc
     8521 cacatgagcc tgtggctggc cggccacggc caccaggtga ccctcgccga accggccgca
     8581 ccgatgctcg acggcgcccg cgcgcgcttc gccgaggccg ggctgcaggc cagcttcatc
     8641 caggcgccgt ggcaggaact gctcggccag ttcgagcaac cgttcgacct agtgctctgc
     8701 catgccgtgc tggaatggct ggccgagccg ccggcgatcc tgccggtgct gcaccagctc
     8761 accgcggcgg acggctggct gtcgctggcc ttctacaacc gcgacgcgct gatctaccgc
     8821 aacctgctca aggggcattt ccgcaagctg cgtaaggagc gcttcgccgg cgagggccag
     8881 agcctgaccc cgcagcgtcc gctcgacccg cgcgagctgg ccgggcagct cgacgactgg
     8941 ttccgcgtcg agcagcagag cggcgtgcgg gtgttccacg actacatgcc ggccgagttc
     9001 caggcccgcg ccgagctgac cgacctgctg gagatggaac tggcgcaccg ccgccatccg
     9061 gccttcgccg ggctcggccg ctacctgcac tggctgtgcc ggccgcagcc ctgagcagcg
     9121 attgccagcg ggcccgcaca gtcgcaagct attcgcagcc ccacccgcta tccggagctg
     9181 ccgccatggc tgtccgcgcg cccaccctgt tgctgctcgt cctcctcagc ggttgccaga
     9241 gcggcaaccc ctacaccgcc agccacctgc cctacccgcc gccgccgcgg gaagcggcgg
     9301 cgctgcaggt cgacccgggc agctatccgc ccacgccgcg cgacttcgcc cagtaccgca
     9361 actggcaatg ggcggcggcg gtcgacgagc cgctggccgg catcgtctct gccgaactcg
     9421 accagcgcgg cctgcgcccg gccaccgcgc agagcccggc caccctgagc gtgcgcgcgc
     9481 agcagcgcag cgtcacccgc cagcagcagg tctacgacga tccgtacttc gacgccggct
     9541 acggccacta cagccatcac cacggctact ggggcggcac cggctacccg ctggtgcgca
     9601 ccatcagcta ccgggtcgac gaggtgcagc tggagctgtt cgatgcccgc agcggcgagc
     9661 gcgtgtggag cggcagcggc gaggccgaaa gcggccgtgc ccgggacgac agcctgcgcc
     9721 gcgcggtacg ccaggcgctg gacggtttcc cgccgccttg accaccgccc ccggcacgac
     9781 cacgcttagt gctacgacgc tgccccggcg cccccctgct tccgggagac ccccatgttc
     9841 cgccgcctgc tcctcatcgc taccctgctc gccctggccg cctgccagag cgcgcagctc
     9901 gacagcgact acgaccccaa ccgcgacttc gccgccctgc agacgtggag ctggcaggag
     9961 ccggccgtgc agtaccgccc ggacgatccg cggctacgca gcgacctgac cgaacagcgg
    10021 ctgcgccagg cggtcgccgc acaactggaa cagcgcggcc tgcgccaggc cgccgcgaac
    10081 gccgcggccg atatccgcgt gcaggccttc ctcatggtcg agaaccgcca gcagcagatg
    10141 gccacctact acggcagcga catgatgagc gaccgctggg gcggccactg ggcctcgccc
    10201 ggctacaccg aggtgcgcac cctcgactat caggtcggca ccctgcaact cgactttctg
    10261 gatcgcgacg gcaaactggt ctggcgcggc agcgcggcgc agatcgtgcc gcgccatgcc
    10321 ggctcgccac aggagcgggc ggcgtcgatc cagcagacgg tggcgaagat cctcgcccaa
    10381 tacccgccgc gggggcgctg agttcgccat gggccagcgc atcctgatcc tggtcgccag
    10441 cggaccgagt acgccgctgc gctgcgccgc gcccttccac atcgccaccc tgctggcgtg
    10501 catgga
//
LOCUS       DATCUN010000100         6203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_22830, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000100 DATCUN010000000
VERSION     DATCUN010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22830"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VIO83_03830"
     CDS             <1..460
                     /locus_tag="VIO83_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX-like family protein"
                     /protein_id="HEY8330440.1"
                     /translation="AEACGYRCLPLAARLHDWPQPADSAGLIVQPLLLALLVVVWLGT
                     ALACLGPGFDWSLRILAEAGIAGWPARIAVVSGALLDGALGLGLLWRRWRRRALQAQI
                     ALMLGYTALITWLLPHYWFDPFQGVSKNLVLLGASLWLLWLQTSSERTRA"
     gene            457..924
                     /locus_tag="VIO83_03835"
     CDS             457..924
                     /locus_tag="VIO83_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HEY8330441.1"
                     /translation="MTLYLLLKTLHILSSTLLFGTGLGSAYYAWRAWKSAQVATIATT
                     FRHLVSADWLFTTPTAVIQPLSGLAMVHLGGWPLSQRWLLVTLILYELAGLCWLPVVW
                     LQIRVRDLAVQAERDGTPLPASAGRYMAWWFALGWPAFMAFVAIFFLMVVKPT"
     gene            complement(980..1636)
                     /locus_tag="VIO83_03840"
     CDS             complement(980..1636)
                     /locus_tag="VIO83_03840"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8330442.1"
                     /translation="MRLALFDLDNTLLAGDSDHAWGDWLCRRGILDEAVYKARNDEYY
                     EDYLAGRLDISAYLNFCLAVLGQYDRAQLDAWHREFMAECIEPIILAKGEALLAEHRA
                     AGDQLVIITATNRFVTGPIAARLGVDTLLATECEMQGEQYTGRTTDIPCFREGKVTRL
                     ERWLLENGLSLEGSCFYSDSRNDLPLLELVDRPVAVDPCAELRAIAEQRGWPVLSLRG
                     "
     gene            1778..2257
                     /locus_tag="VIO83_03845"
     CDS             1778..2257
                     /locus_tag="VIO83_03845"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEY8330443.1"
                     /translation="MIDPDGFRPNVGIILANEVGQVMWARRINQDAWQFPQGGINPQE
                     SPEDALFRELNEEVGLEPEDVRIIACTRGWLRYRLPQRLVRTHSQPLCIGQKQKWFLL
                     RLTGDERRVRMDLTGKPEFDDWRWVSYWYPLGQVVSFKREVYRRALKELAPRLPLRD"
     gene            2308..4587
                     /gene="ptsP"
                     /locus_tag="VIO83_03850"
     CDS             2308..4587
                     /gene="ptsP"
                     /locus_tag="VIO83_03850"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY8330444.1"
                     /translation="MLHTLRKIIQEVNSARDLDTALTIIVERVRAAMGTQVCSVYLLD
                     PQSNRFVLMATEGLNKASIGQVSMAPSEGLVGLVGTREEPLNLEDAASHPRFRYFAET
                     GEERYASFLGAPIIHHRRVMGVLVVQQKERRQFDEGEEAFLVTMSAQLAGVIAHAEAT
                     GSIRGLGKQGKAIEDTRFVGVPGSPGVALGSAVVVLPAADLEVVPDRQVTDIDGEIAY
                     FYNALEAVRADIHALSARLASQLRQQERALFDVYLMMLDDASLGGEVIKVIETGQWAQ
                     GALRQVVNGHVQRFEQMDDAYLSERASDVRDIGRRLLAYLQQARQQKLVYPDNTILVS
                     EELSPAMLGEVPPGKLVGLVSVRGSSNSHVSILARAMGIPTVMGAADLPYSMVDGIEL
                     IVDGYRGDVFTNPSPGLRRQFAEIAEEERQLNLGLESLRSLPCETLDGHRLPLWANTG
                     LFADIARAHERGAEGVGLYRSEVPFMTNERFPSEKEQVALYRQQLAAFHPLPVTMRTL
                     DIGGDKALSYFPIKEDNSFLGWRGIRITLDHPEIFLVQARAMLKASEGLDNLRILLPM
                     ISGLPELEEAQRLLHRAWSEVREEGVDVPMPPVGVMIEIPAAVFQTRELARQVDFLSV
                     GSNDLTQYLLAVDRNNPRVANLYDYLHPSILHALKRVVDDAHSVGKPVSICGEMAGDP
                     SAAMLLMAMGFDSLSMNATNLPKVKWLLRQVSLQQARELLDQVLLIDHAPVIHSTLSL
                     ALRHLGLARMLNPGASSQS"
     gene            4657..5439
                     /locus_tag="VIO83_03855"
     CDS             4657..5439
                     /locus_tag="VIO83_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8330445.1"
                     /translation="MEFLLYLLLGGAAGVLAGLFGVGGGMIMVPVLIYSFQAQGVAPE
                     VLTHLAVGTSLATIAFTSINSIRAHHKRGAVRWEIFRWLAVGILGGSVLGGLTASLLQ
                     GPLLQKIIGVFAIVMAAQMAFNLQPQASSAVPGKAGLGVAGTVIGWASAIIGIGGGSL
                     TVPFLSWRSVPMQQAVATSAACGLPIAVASALSFMWLGHNEPQLPEWSIGFVYLPAVV
                     GIASTSMFFARFGAQLAHRLSPLMLKRLFALLLLSVGLNFLL"
     gene            5449..>6203
                     /gene="lgt"
                     /locus_tag="VIO83_03860"
     CDS             5449..>6203
                     /gene="lgt"
                     /locus_tag="VIO83_03860"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY8330446.1"
                     /translation="MLVYPQIDPVAIALGPLKIHWYGLMYLIGIGGAWWLASRRLARF
                     DATWTRDTLSDLVFWAACGVILGGRFGYVVFYNFEQFLQDPLWLLRVWEGGMSFHGGF
                     LGVMLAMWWFGKRHGKSLFELLDFIAPMVPIGLGAGRIGNFINAELWGKASDVPWAMV
                     FPAWSDPQQLARHPSQLYQAALEGVALFAILWLYSRKPRPTMAVSGLFATCYGLFRFI
                     VEFVRVPDAQLGYLAFGWLTMGQVLSLPMILGGL"
BASE COUNT          890 a         2209 c         2054 g         1050 t
ORIGIN      
        1 ggccgaggcc tgcggctacc gctgcctgcc gctggccgcg cgcctgcatg actggccgca
       61 gccggcggac agcgccggcc tgatcgtgca gccgctgctg ctggcgctgc tggtagtggt
      121 ctggctgggc accgcgctgg cctgcctcgg ccccggcttc gactggagcc tgcgcatcct
      181 cgccgaagcg ggcatcgccg gctggccggc acggatcgcc gtggtcagcg gtgcgctgct
      241 cgatggcgca ctcggcctcg gcctgctgtg gcggcgctgg cgccgccgcg ccctgcaggc
      301 gcagatcgcc ctgatgctcg gctacacggc gctgatcacc tggctgctgc cgcactactg
      361 gttcgatccg ttccagggcg tcagcaagaa cctggtgctg ctcggcgcca gcctatggtt
      421 gctgtggctg cagacctcta gcgaacggac tcgcgcatga ccctctacct gctgctcaag
      481 accctgcaca tcctctcctc caccctgctg ttcggcaccg gcctcggctc ggcctactac
      541 gcctggcggg cgtggaagag cgcccaggtg gcgaccatcg ccaccacctt ccgccacctg
      601 gtcagcgccg actggctgtt caccaccccg accgcggtta tccagccgct cagcggcctg
      661 gccatggtcc atctgggcgg ctggccgctg agccagcgct ggctgctggt caccctcatc
      721 ctctatgagc tggccgggct gtgctggctg ccggtggtgt ggctgcagat ccgcgtgcgc
      781 gacctggcgg tgcaggccga acgcgacggc acgccgctgc cggccagcgc cgggcgctac
      841 atggcctggt ggttcgctct cggctggccg gcattcatgg ccttcgtggc gatctttttc
      901 ctgatggtgg tcaagccaac ctgaatccgc aaacgaagac ggcgcccatg ggcgccgtct
      961 tcgttgatgt caccgggcgt cagccgcgca gcgagagcac cggccagccg cgctgctcgg
     1021 cgatggcgcg cagttccgcg cagggatcga cggccaccgg gcggtcgacc agctccagca
     1081 gcggcaggtc gttgcgcgag tcgctataga agcagctgcc ctcgaggctc aggccgtttt
     1141 ccagcagcca gcgctccagg cgggtcacct tgccctcgcg gaagcagggg atatcggtgg
     1201 tgcgcccggt gtactgctca ccctgcatct cgcactcggt ggccagcagg gtgtcgacgc
     1261 cgaggcgcgc ggcaatcggc ccggtgacga agcggttggt ggcggtgatg atcaccagct
     1321 ggtcgccggc ggcgcggtgc tcggccagca gcgcctcgcc cttggcgagg atgatcggct
     1381 cgatgcactc ggccatgaac tcgcggtgcc aggcgtccag ctgcgcccgg tcgtactggc
     1441 cgagcacggc caggcagaag ttcaggtagg cgctgatgtc caggcgcccg gccaggtagt
     1501 cctcgtagta ctcgtcgttg cgcgccttgt agacggcttc gtcgaggatg ccgcgccggc
     1561 acagccagtc gccccaggcg tggtcgctgt cgccggccag cagggtgttg tcgagatcga
     1621 atagcgccag acgcacggat aggctctctc aaggcaggat gggaatgggc tagcgtaccc
     1681 cgttttggct ggactgccca tgcgcggacg cacgcgcgtt gccgctgtga cgggctttgt
     1741 ggaacaatcc gggcatatat gatttgaggt gcctgccatg atcgatcccg atggcttccg
     1801 accgaatgtc ggcatcattc tggccaatga ggtcggccag gtgatgtggg cgcggcgcat
     1861 caaccaggat gcctggcagt tcccgcaggg cgggatcaac ccgcaggaat cgccggaaga
     1921 tgcgctgttt cgcgaactga atgaagaagt gggcctcgag cccgaagatg tgcgcatcat
     1981 cgcctgcacc cgcggctggt tgcgctatcg tttgccgcag cgtctggtgc gcacccacag
     2041 ccagccgctg tgcatcggcc agaagcagaa gtggttcctg ctgcgcctga ccggcgacga
     2101 acggcgggtg cgcatggacc tgaccggcaa gccggagttc gacgactggc gctgggtcag
     2161 ctactggtac ccgctgggtc aggtcgtttc cttcaagcgc gaggtgtatc gtcgtgccct
     2221 gaaggagctg gccccgcgcc tgccgcttcg cgactgacca ccaccacccg accaccgact
     2281 gctactggga gcgacgcccc tttgaccatg ctccacacgc tgcgcaagat catccaggag
     2341 gtcaactccg cccgggatct ggacaccgcc ctgacgatca tcgtcgagcg cgtgcgcgca
     2401 gccatgggca cccaggtctg ctcggtgtac ctgctcgatc cgcagagcaa tcgcttcgtg
     2461 ctgatggcca ccgagggcct caacaaggcc tccatcggcc aggtcagcat ggcccccagc
     2521 gaaggtctgg tcggcctggt cggcacccgc gaggagccgc tcaacctcga ggacgccgcc
     2581 agccacccgc gtttccgcta cttcgccgag accggcgaag agcgctacgc ctccttcctc
     2641 ggcgcgccga tcatccacca ccggcgggtg atgggcgtgc tggtggtgca gcagaaggag
     2701 cgccgccagt tcgacgaggg cgaggaagcc ttcctggtca ccatgagcgc ccagctcgcc
     2761 ggggtcatcg cccacgccga ggccaccggc tcgatccgcg gcctcggcaa gcagggcaag
     2821 gccatcgagg acacccgctt cgtcggcgtg cccggctcgc ccggcgtggc cctgggcagc
     2881 gccgtggtgg tgctgccggc cgccgacctt gaagtggtgc cggaccgcca ggtgaccgac
     2941 atcgacggcg agatcgccta cttctacaac gccctcgagg cggtgcgcgc cgacatccat
     3001 gcgctgtccg cgcgcctggc cagccagctg cgccagcagg agcgcgcgct gttcgacgtc
     3061 tacctgatga tgctcgacga cgcctcgctc ggcggcgaag tgatcaaggt gatcgagacc
     3121 ggccagtggg cgcagggcgc cctgcgccag gtggtcaacg gccacgtgca gcgcttcgag
     3181 cagatggacg acgcctacct cagcgagcgc gcctcggatg tgcgcgacat cggccggcgc
     3241 ctgctcgcct atctgcagca ggcccgccag cagaagctgg tatacccgga caacaccatc
     3301 ctggtcagcg aggagctgtc gccggcgatg ctcggcgaag tgccgcccgg caagctggtc
     3361 ggcctggttt cggtacgcgg ctcgagcaac tcgcacgtct ccatcctcgc ccgcgccatg
     3421 ggcatcccca cggtgatggg cgcggccgac ctgccctaca gcatggtcga cggcatcgag
     3481 ctgatcgtcg atggctaccg cggcgacgtg ttcaccaacc cctcgcccgg cctgcgccgc
     3541 cagttcgccg agatcgccga ggaagagcgc cagctcaacc tcggcctcga atccctgcgc
     3601 agcctgccct gcgaaacgct cgacggccac cgcctgccgc tgtgggccaa caccggcctg
     3661 ttcgccgaca tcgcccgcgc ccacgagcgc ggcgccgagg gcgtcggcct gtaccgcagc
     3721 gaagtgccgt tcatgaccaa cgagcgcttc cccagcgaga aggagcaggt cgccctgtac
     3781 cgccagcagc tggccgcctt ccacccgctg ccggtgacca tgcgtaccct cgacatcggc
     3841 ggcgacaagg cgctgtcgta cttcccgatc aaggaggaca actccttcct cggctggcgc
     3901 ggcatccgca tcaccctcga ccacccggaa attttcctgg tccaggcgcg cgccatgctc
     3961 aaggccagcg aggggctgga caacctgcgc atcctgctgc cgatgatcag cggcctgccc
     4021 gagctggaag aggcgcagcg cctgctgcac cgcgcctgga gcgaagtgcg cgaggaaggc
     4081 gtcgacgtgc cgatgccgcc ggtgggggtg atgatcgaga ttcccgccgc ggtgttccag
     4141 acccgcgagc tggcccgcca ggtcgacttc ctctcggtcg gctccaacga cctgacccag
     4201 tacctgctgg ccgtggaccg caacaacccg cgggtggcca acctctacga ctacctgcac
     4261 ccgtcgatcc tgcacgccct caagcgcgtg gtcgacgacg cccacagcgt cggcaagccg
     4321 gtgagcatct gcggcgagat ggccggcgac ccgagcgccg ccatgctgct gatggccatg
     4381 ggcttcgact cgctgtcgat gaacgccacc aacctgccca aggtgaagtg gctgctgcgc
     4441 caggtctccc tgcagcaggc ccgcgagctg ctcgaccagg tgctgctcat cgaccatgcc
     4501 ccggtgatcc acagcaccct cagtctggcc ctgcgccacc tcggcctggc acgcatgctc
     4561 aacccgggcg cctccagcca gagctgaagg ccggccgccg cagctgcggg cggccggcgc
     4621 ttccgctacc atagccggcc gttctggagg ccatcgatgg aatttcttct ctacttgctg
     4681 ctgggtggcg cagccggcgt gctggccggc ctgttcggcg tcggcggcgg catgatcatg
     4741 gtgccggtgc tgatctacag cttccaggcc cagggcgtcg cccccgaagt gctcacccac
     4801 ctggcggtcg gcacctcgct ggcgaccatc gccttcacct cgatcaactc gatccgcgcc
     4861 caccacaagc gtggcgcggt gcgctgggag atcttccgct ggctggccgt cggcatcctc
     4921 ggcggcagcg tgctcggcgg cctgaccgcc tccctgctgc agggcccgct gctgcagaag
     4981 atcatcggcg tgttcgccat cgtcatggcc gcgcagatgg ccttcaacct gcagccccag
     5041 gccagctccg cggtacccgg caaggccggg ctcggcgtcg ccggcacggt gatcggttgg
     5101 gcctcggcga tcatcggcat cggcggcggc tcactgaccg tgcccttcct cagctggcgc
     5161 agcgtgccga tgcagcaggc ggtggccacc tcggcggcct gcggcctgcc gatcgccgtg
     5221 gccagcgcgc tgagcttcat gtggctgggc cacaacgagc cgcagctgcc ggagtggagc
     5281 atcggcttcg tctacctgcc ggcggtggtc ggcatcgcct ccacctcgat gttcttcgcc
     5341 cgcttcggcg cgcagctggc ccaccgcctg tcaccgctca tgctcaagcg cctgttcgcc
     5401 ctgctgctgc tgagcgtggg cctgaatttc ctgctctgag gacctgacat gctggtttac
     5461 ccgcagatcg acccggtggc catcgccctc ggcccgctga agatccactg gtacggcctg
     5521 atgtacctga tcggcatcgg cggcgcctgg tggctggcca gccgccggct ggcccgcttc
     5581 gacgccacct ggacccgcga caccctgtcc gacctggtgt tctgggccgc ctgcggggtg
     5641 attctcggcg gccgcttcgg ctacgtggtg ttctacaact tcgagcagtt cctccaggac
     5701 ccgctgtggc tgctgcgcgt ctgggaaggc ggcatgtcgt tccacggcgg cttcctcggc
     5761 gtgatgctgg ccatgtggtg gttcggcaag cgccacggca agagcctgtt cgaactgctg
     5821 gacttcatcg ccccgatggt gccgatcggc ctcggcgccg ggcgcatcgg caacttcatc
     5881 aacgccgaac tgtggggcaa ggccagcgat gtgccgtggg cgatggtgtt cccggcgtgg
     5941 agcgatccgc agcagctggc gcgccacccg tcgcagctgt accaggccgc gctggagggc
     6001 gtggcgctgt tcgccatcct ctggctgtac tcgcgcaagc cgcgaccgac catggcggtg
     6061 tccggcctgt tcgccacctg ttacggcctg ttccgcttta tcgtcgaatt cgtccgcgtg
     6121 ccggatgccc agctcggcta cctcgccttc ggctggctga ccatgggcca ggtcctcagc
     6181 ctgccgatga tcctcggcgg cct
//
LOCUS       DATCUN010000101         3037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_267023, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000101 DATCUN010000000
VERSION     DATCUN010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267023"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1081
                     /gene="fba"
                     /locus_tag="VIO83_03865"
     CDS             17..1081
                     /gene="fba"
                     /locus_tag="VIO83_03865"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249246.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY8330447.1"
                     /translation="MALISMRQMLDHAAEFGYGVPAFNVNNLEQMRAIMEAADKTDSP
                     VIVQASAGARKYAGAPFLRHLILAAIEEFPHIPVCMHQDHGTSPDVCQRSIQLGFSSV
                     MMDGSLMADGKTPADYDYNVRVTQQTVAMAHACGVSVEGELGCLGSLETGQAGEEDGV
                     GAEGTLDHSQMLTDPEEAAAFVKATQVDALAIAIGTSHGAYKFTKPPTGDVLAIERIK
                     EIHKRIPNTHLVMHGSSSVPQEWLAIINQYGGDIKETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAMRRMMAEQPSEFDPRKFFAQTIVAMRDICIARYEAFGTAGNASKIKP
                     ISLEGMYQRYAKGELNAKVN"
     gene            complement(1256..1651)
                     /locus_tag="VIO83_03870"
     CDS             complement(1256..1651)
                     /locus_tag="VIO83_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330448.1"
                     /translation="MTSEAIAQQPHQEFMHRESDGCSCAICGTTARPLAFHVLQQSYS
                     RDDAEPKGFMVLSMSVDAVHGIFPVCDRCAPACSTCGRPVASERVLEFARVQGANLGD
                     GACQQHAQLGERLKAAFKRTFKLGRFKNA"
     gene            1950..2408
                     /locus_tag="VIO83_03875"
     CDS             1950..2408
                     /locus_tag="VIO83_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330449.1"
                     /translation="MDEFNPYAPPRSRDPAPLDPSAAPPGFAVYRWLNWLYAGVMLVA
                     LLILLGGGTLRAEFSHLLVVAIFLAPSFSFALVLARRAAWFRRWSWVQGLGSAVLLLF
                     CFLDITGGHGLRGIGVFMTAVNAAALFAGEHFLRLQRTPGMPAESPQRHA"
     gene            2462..2959
                     /locus_tag="VIO83_03880"
     CDS             2462..2959
                     /locus_tag="VIO83_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007969689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY8330450.1"
                     /translation="MTPLKYLNAYPSQLQDQVRRLLAEQRLGDYLHKRYPGRHTVQSD
                     KALYAYTMELKQQYLRNAPGIDKVLYDSKLDVVRNALGLHTAVSRVQGGKLKAKKEIR
                     VAALFREAAPEFLQMIVVHELAHLREFDHNKAFYQLCEHMLPGYHQLEFDLRAYLTWR
                     EQAAG"
BASE COUNT          488 a         1037 c         1016 g          496 t
ORIGIN      
        1 actgggagca ccacagatgg cacttatcag catgcgccag atgctggatc acgccgccga
       61 attcggctac ggcgtgccgg ccttcaacgt caacaacctc gagcagatgc gcgccatcat
      121 ggaagcggcc gacaagaccg actccccggt gatcgtccag gcctccgccg gcgcgcgcaa
      181 gtacgccggt gcgcccttcc tgcgccacct gatcctcgct gccatcgaag aattcccgca
      241 catcccggtg tgcatgcacc aggaccacgg caccagcccg gacgtctgcc agcgctcgat
      301 ccagctgggc ttcagctcgg tgatgatgga cggctcgctg atggccgacg gcaagacccc
      361 ggccgactac gactacaacg tgcgcgtcac ccagcagacc gtggccatgg cccacgcctg
      421 cggcgtgtcg gtggaaggcg agctgggctg cctgggcagc ctggaaaccg gccaggccgg
      481 tgaagaggac ggcgtcggcg ccgagggcac cctcgaccac agccagatgc tcaccgatcc
      541 ggaagaagcg gcggccttcg tcaaggccac ccaggtcgac gccctggcca tcgccatcgg
      601 caccagccac ggcgcctaca agttcaccaa gccgccgacc ggcgacgtgc tggccatcga
      661 gcgcatcaag gaaatccaca agcgcatccc caacacccac ctggtgatgc acggctcttc
      721 ctcggtgccg caggagtggc tggcgatcat caaccagtac ggtggcgaca tcaaggaaac
      781 ctacggcgtg ccggtcgagg aaatcgtcga gggcatcaag cacggcgtgc gcaaggtcaa
      841 catcgacacc gacctgcgcc tggcctccac cggcgccatg cgccgcatga tggccgagca
      901 gccgagcgag ttcgacccgc gcaagttctt cgcccagacc atcgtggcca tgcgcgacat
      961 ctgcatcgcc cgctacgaag ccttcggcac cgccggcaat gcctcgaaga tcaagccgat
     1021 ctccctggaa ggcatgtacc agcgctacgc caagggcgag ctgaacgcca aggtcaactg
     1081 atcaacgcgt cctgccgcaa tcccacaagg cccgcctcgc gcgggccttg tgcattctgg
     1141 cgggtaactc ggcggcgcct gcgcatggct gcgctggggg gggattcgga gggcggcgct
     1201 ggggcctgag cgggcaggca gccgctcgcg gcgagcggcc ggggcgggct gggggctagg
     1261 cgttcttgaa gcggcccagc ttgaaggtcc gcttgaaggc ggccttgagg cgttcaccga
     1321 gctgggcgtg ctgctggcag gcgccatcgc cgagattggc gccctggacc cgggcgaact
     1381 cgagcacgcg ctcgctggcg accggacggc cacaggtcga gcaggccggc gcacagcggt
     1441 cgcacaccgg gaagatcccg tgcacggcgt cgacggacat ggacagcacc atgaacccct
     1501 tgggttcggc atcgtcccgc gagtaggact gttgcaggac atgaaaggcc agcgggcgag
     1561 cggtggtgcc gcaaatggca caggagcacc catcgctttc gcgatgcata aactcttgat
     1621 ggggctgctg ggcgattgct tcggaggtca taacgtcttt tcctggttgg agttccatgg
     1681 cttgaattgc gcggttccac tgccggcggg gctctggccc ggccggcgtg gatcgcgagt
     1741 caagcactca gccgcgccgc gcaggctggc ggactgatgt gccgcggcgc tgagtggcgc
     1801 cgctggcggt tgtcgtcggt tttgctgtat tcaagcacgt ttcacccctc gggggtgcct
     1861 attgcgccgc gctgcgttgt tgcgcgtgca gccgcagccc tgccggcgcg ctatagtcgg
     1921 ccccgtgttc cgctgccaga cctgcactca tggacgagtt caacccctat gccccgccgc
     1981 gctcgcgcga tcccgcgccc ctcgatccgt ccgccgcgcc acccggcttc gcggtctatc
     2041 gctggctgaa ctggctgtat gccggcgtca tgctggtcgc cctgctgatc ctgctcggcg
     2101 gtggcaccct gcgcgcggag ttctcccatc tgctggtggt ggcgatcttc ctcgcgccgt
     2161 cgttcagctt tgccctggtg ctcgcgcggc gcgcggcctg gttccggcgc tggtcctggg
     2221 tccagggcct gggcagcgcc gtgttgctgc tgttctgttt tctcgacatc accggcggcc
     2281 acgggctgcg cggcatcggc gtgttcatga cggcggtgaa cgccgccgcg ctgttcgccg
     2341 gcgaacactt cctgcgcctg cagcggacgc cgggcatgcc ggcggagagt ccccagcggc
     2401 acgcctgaag cggtcgcggc cgggtagaat gagcgcttcc tcgcctgccg ccccgctgcc
     2461 catgaccccg ctcaaatacc tcaacgccta tccctcccag ctgcaggacc aggtgcgccg
     2521 gctgctcgcc gaacagcgcc tgggcgacta cctgcacaag cgctatccgg ggcgtcacac
     2581 ggtacagagc gacaaggcgc tgtacgccta caccatggag ctcaagcagc agtacctgcg
     2641 caacgcgccg ggcatcgaca aggtgctcta cgacagcaag ctggatgtgg tgcgcaatgc
     2701 gctcggcctg cataccgcgg tctcgcgggt gcagggcggc aagctcaagg ccaagaagga
     2761 gatccgtgtg gccgcgctgt ttcgcgaggc ggcgccggag ttcctgcaga tgatcgtggt
     2821 gcacgagctg gcgcatctgc gcgagttcga ccacaacaag gcgttctacc agctgtgcga
     2881 gcacatgctg ccgggctatc accagctgga gttcgacctg cgcgcgtacc tgacctggcg
     2941 cgagcaggcg gcgggctgat ggccgcgtag cccgcgtcag cgccgggtgt ggctggatca
     3001 gggctgttgc ggggcttgcc ccttggcgct gcgtggt
//
LOCUS       DATCUN010000102         6537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_154636, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000102 DATCUN010000000
VERSION     DATCUN010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154636"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..84)
                     /locus_tag="VIO83_03885"
     CDS             complement(<1..84)
                     /locus_tag="VIO83_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8330451.1"
                     /translation="MSYHELSATERVTIQIGLCNGFSQRRLA"
     gene            complement(139..1068)
                     /gene="dmpG"
                     /locus_tag="VIO83_03890"
                     /pseudo
     CDS             complement(139..1068)
                     /gene="dmpG"
                     /locus_tag="VIO83_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448357.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
     gene            complement(1080..2018)
                     /locus_tag="VIO83_03895"
     CDS             complement(1080..2018)
                     /locus_tag="VIO83_03895"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019829305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HEY8330452.1"
                     /translation="MSKKFKAAIIGSGNIGTDLMIKILRHGQHLEMGAMVGIDPASDG
                     LARAARMGVATTHEGVEGLTRLDVFKDIDFVFDATSAGAHVKNDAFLRSLKPGIRLID
                     LTPAAIGPYCVPVVNLEEHLDALNVNMVTCGGQATIPMVAAVSRVAKVHYAEIVASIA
                     SKSAGPGTRANIDEFTETTTQAIEVIGGAAKGKAIIVMNPAEPPLIMRDTVFVLSEAV
                     DQAKVEASVEEMAQAVQAYVPGYRLKQRVQFDVIPEDKPLTIPGHGQFSGLKTSVFLE
                     VEGAAHYLPAYAGNLDIMTSAALATAERMALSMLNA"
     gene            complement(2033..2815)
                     /gene="dmpE"
                     /locus_tag="VIO83_03900"
     CDS             complement(2033..2815)
                     /gene="dmpE"
                     /locus_tag="VIO83_03900"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017243903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008684 - 2-oxopent-4-enoate hydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxopent-4-enoate hydratase"
                     /protein_id="HEY8330453.1"
                     /translation="MNIELINQLGDELYQAMLSREAVAPLTSRFDLTIDDAYQISLRM
                     LERRLAAGERIIGKKIGLTSKAVQTMLGVNQPDFGYLTDAMVFSSGAVMPISDRLIQP
                     KAEGEIAFILKKDLMGPGITNADVLAATECVIPCFEVVDSRIQDWKIKIQDTVADNAS
                     CGLFILGDQAVSPRQVDLVTCGMVVEKNGQQISTGAGAAALGSPVNCVAWLANTLGHF
                     GIGLKAGEVILSGSLVPLEPVKAGDFMRVDIGGIGSASVRFV"
     gene            complement(2937..4397)
                     /locus_tag="VIO83_03905"
     CDS             complement(2937..4397)
                     /locus_tag="VIO83_03905"
                     /EC_number="1.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconic semialdehyde dehydrogenase"
                     /protein_id="HEY8330454.1"
                     /translation="MKEIKHFINGEYVGSVSGKLFDNVNPANGQVIAKIHEAGEAEVD
                     AAVKAARAALKGPWGKMTVAERTEILHRVAAGITARFDEFLEAECLDTGKPKSLASHI
                     DIPRGAANFSVFADLVKNVPTEAFEMATPDGTGALNYGVRRPKGVIGVISPWNLPLLL
                     MTWKVGPALACGNTVVVKPSEETPTTTALLGEVMNAAGVPAGVYNVVHGFGGNSAGAF
                     LTAHPDVDAITFTGETGTGETIMRAAAKGVRQVSLELGGKNAGIVFADCDMDKAIEGT
                     LRSAFANCGQVCLGTERVYVQRPIFDEFVARLKAGAEALKIGEPNDPSANFGSLVSHK
                     HREKVLSYYQKAKDEGATIVTGGGVPDMPAHLAGGAWVQPTIWTGLADDSAVVTEEIF
                     GPCCHIRPFDSEEEAIELANSLPYGLASAIWTENSSRAHRVAGQIEAGIVWVNSWFLR
                     DLRTAFGGAKQSGIGREGGVHSLEFYTELKNICVKL"
     gene            complement(4421..5344)
                     /locus_tag="VIO83_03910"
     CDS             complement(4421..5344)
                     /locus_tag="VIO83_03910"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011005909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HEY8330455.1"
                     /translation="MKKGVMRPGHIQLRVLDMAKALEHYVELLGLIEVDRDDKGRVYL
                     KGWTEVDKFSVVLREADEAGMDFMGFKVINEAALGQLTEDLVAFGCAVESIPADELKG
                     CGRRIRFSAPSGHCFELFAEKQQTGKWGIAEINPEAWPRGLKGIKATRFDHALLYGDE
                     LAETLRLFTEVLGFDLAEQVLDPEGTRVAQFLTCSMKAHDVAFIQHAEKGKFHHASFF
                     LETWDDVLRAADLISMTDTSIDIGPTRHGLTHGKTIYFFDPSGNRNEVFCGGDYHYPD
                     HPPVTWNAADLGKAIFYHDRQLNERFLTVLT"
     gene            complement(5409..5534)
                     /locus_tag="VIO83_03915"
     CDS             complement(5409..5534)
                     /locus_tag="VIO83_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330456.1"
                     /translation="MRVLHGEYHCEPMSSRHAPAAAHAAGGGAGLPAVSAERPHD"
     gene            complement(5817..6047)
                     /locus_tag="VIO83_03920"
                     /pseudo
     CDS             complement(5817..6047)
                     /locus_tag="VIO83_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009397177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="salicylate 1-monooxygenase"
     gene            complement(6231..6408)
                     /locus_tag="VIO83_03925"
                     /pseudo
     CDS             complement(6231..6408)
                     /locus_tag="VIO83_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003176541.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde dehydrogenase,
                     glutathione-independent"
BASE COUNT         1014 a         2015 c         2275 g         1233 t
ORIGIN      
        1 ggcaagcctg cgctggctga agccattgca caggccgatc tggatcgtga cgcgctctgt
       61 ggcgctgagt tcgtgatagg acatggggca acaccttacc gaaatggtcg ggtgttgcac
      121 tcagtttttg cggccgccca gggtcttcag accgtacttc tcggcggcca cttcggcgtg
      181 gcgcaggaag ctcgagtaca cgccggcgta gccgaggccg agggtctcgc ggtcgacgcg
      241 caccgggcgg tcctgcagcg ggcgcaccag gtcgtcggcg gcgtccatca gccggtacag
      301 gtcggtgccg tggttccagc ccaggcgctc ggcggcggcg atgaacactt ccagcggcgc
      361 gttgccggcg ccggcgccca tgcctgcgag gctggcgtcg atgcggtcgc agccctcttc
      421 caccgcggtg atcgagttgg ccacgccgag gctgaggttg tggtgggcgt gcatgccggt
      481 ctgggtttcc gggttcagca cggccttgaa cgcgcgcatg cggtcgcgga tgtcctgcat
      541 gttcatcgcc ccgccggagt cggccatgta gatgcactgc gcgccgtagc tttccatcag
      601 cttgccctgc gcggccagct gctcggcggg gatcatgtgg ctcatcatca ggaagccgac
      661 ggtgtccatg ccgagcgaac gggcgtattc gatgtgctgc ttggagacgt ccgcctcggt
      721 gcaatgggtg gcgatgcgca ccgaacgggc gccggcgtcg taggcggctt tcagatcatg
      781 gacggtgccg atgccgggca gcagcagcac ggtgatcttg gcgtgctcga tcacgtcggc
      841 ggcggcctcg atccactcca ggtcggtgtg ggcgccgaag ccgtagttga aggaggaacc
      901 ctgcaggccg tcgccgtggg tgacttcgat ggagtcgacc ttggcctcgt ccagcgcgcg
      961 ggcgatgtcc tgcacgttct ggatcgagta ctggtggcgc acggcgtggc tgccgtcgcg
     1021 cagggtgacg tcggagatgt acagcttctt gctcgggtcg aaggtcatgg cgggtctcct
     1081 tacgcgttca gcatcgacag cgccatgcgc tcggcggtgg ccagcgcagc ggaggtcatg
     1141 atgtcgaggt tgccggcgta ggccggcagg tagtgggcgg cgccttcgac ctcgagaaat
     1201 accgaggtct tcaggccgga gaactggccg tgaccgggga tggtcagcgg cttgtcctcg
     1261 gggatcacgt cgaactgcac cctctgcttg aggcggtagc ccggcacgta ggcctgcacg
     1321 gcctgggcca tttcctcgac gctggcctcg accttggcct ggtcgaccgc ttcggagagc
     1381 acgaacacgg tgtcgcgcat gatcagcggc ggctcggccg ggttcatgac gatgatcgcc
     1441 ttgcccttgg cggcgccgcc gatcacttcg atggcctggg tggtggtctc ggtgaactcg
     1501 tcgatattgg cgcgggtgcc cgggccggcc gacttggagg cgatcgaggc gacgatctcg
     1561 gcgtagtgca ccttagcgac gcgggacacc gcagccacca tcgggatggt ggcctggccg
     1621 ccgcaggtga ccatgttgac gttcaacgca tccaggtgct cttccaggtt gaccaccggc
     1681 acgcagtacg ggccgatggc tgccggggtc aggtcgatca ggcggatgcc gggcttgagc
     1741 gagcgcagga aggcgtcgtt cttcacgtgc gcaccggccg aggtggcgtc gaagacgaag
     1801 tcgatgtcct tgaacacgtc caggcgggtc aggccttcga caccctcgtg ggtggtggcc
     1861 acgcccatgc gcgcggcgcg ggccagaccg tcggaggccg ggtcgatgcc gaccatggcg
     1921 cccatttcca ggtgctggcc gtgacggagg atcttgatca tcaggtcggt gccgatgttg
     1981 ccggagccga tgatcgcggc cttgaatttc ttgctcatgt tcaggctccc gatcagacga
     2041 agcgcacgct ggcgctgccg atgccgccga tgtcgacgcg catgaagtcg ccggccttga
     2101 ccggctccag cggtaccagc gagccggaga ggatcacttc gccggccttc aggccgatgc
     2161 cgaagtggcc gagggtgttg gccagccagg ccacgcagtt gaccggcgag cccagcgcgg
     2221 cggcgccggc gccggtgctg atctgctggc cgttcttctc caccaccatg ccgcaggtga
     2281 ccaggtcgac ctggcgcggc gagacggcct ggtcgccgag gatgaacagg ccgcaggagg
     2341 cgttgtcggc gacggtgtcc tggatcttga tcttccagtc ctggatgcgc gagtcgacca
     2401 cctcgaagca ggggatcacg cactcggtag cggccagcac gtcggcgttg gtgatgccgg
     2461 ggcccatcag gtccttcttg aggatgaagg cgatctcgcc ctcggccttg ggctggatca
     2521 ggcggtcgct gatcggcatc accgcgccgc tgctgaacac catggcgtcg gtcaggtagc
     2581 cgaagtccgg ctggttgacg ccgagcatgg tctgcacggc cttgctggtc aggccgatct
     2641 tcttgccgat gatgcgctcg ccggcggcca ggcgtcgctc gagcatgcgc agggagatct
     2701 ggtaggcgtc gtcgatggtc agatcgaaac ggctggtcag cggggcgaca gcctcgcggc
     2761 tgagcatggc ctggtagagc tcgtcgccga gctggttgat cagttcgata ttcatgcaaa
     2821 gggttcctga tgggtggcgg ggtccgctgg caccggactg cgggcaggcc agcgggccgg
     2881 ggccggcgag gcgggacgga gtcgcccgcg cctcgcggcc gtggttcatg gctggcttac
     2941 agcttcacgc agatgttctt cagctcggtg tagaactcca gcgagtgcac cccgccttcg
     3001 cgaccgatgc ccgactgctt ggcgcctccg aaggcggtgc gcaggtcgcg caggaaccag
     3061 ctgttgaccc agacgatgcc cgcctcgatc tgcccggcga cgcggtgcgc gcgcgagctg
     3121 ttctcggtcc agatcgccga ggccaggccg tagggcaggc tgttggccaa ctcgatggct
     3181 tcctcttcgc tgtcgaacgg acggatgtgg cagcacgggc cgaagatttc ctcggtgacc
     3241 accgccgagt cgtcggccag gccggtccag atggtcggct gcacccaggc gccgccggcc
     3301 aggtgcgccg gcatgtccgg cacgccgccg ccggtgacga tggtggcgcc ctcgtccttg
     3361 gccttctggt agtagctgag caccttctcg cggtgcttgt ggctgaccag ggagccgaag
     3421 ttggcgctcg ggtcgttcgg ctcgccgatc ttcagtgcct cggcgccggc cttcaggcgg
     3481 gcgacgaatt cgtcgaagat cggccgctgc acgtagaccc gctcggtgcc caggcacacc
     3541 tggccgcagt tggcgaacgc cgagcgcagg gtgccctcga tggccttgtc catgtcgcag
     3601 tcggcgaaca cgatgccggc gttcttgccg cccagctcca gggagacctg gcgcacgccc
     3661 ttggcagcgg cgcgcatgat ggtctcgccg gtgccggtct cgccggtgaa ggtgatggcg
     3721 tcgacgtccg ggtgggcggt gaggaaggca ccggccgagt tgccgccgaa gccgtgcacc
     3781 acgttgtaca cgccggccgg cacgccggcg gcgttcatca cctcaccgag gagggcggtg
     3841 gtggtagggg tttcctcgga cggcttgacc accacggtgt tgccgcaggc cagcgccggg
     3901 ccgaccttcc aggtcatcag cagcagcggc aggttccacg ggctgatcac gccgatcacg
     3961 cccttggggc ggcgcacgcc gtagttgagc gcgccggtgc cgtccggggt ggccatctcg
     4021 aaggcctcgg tgggcacgtt cttgaccagg tcggcgaaca ccgagaagtt ggccgcgccg
     4081 cgcgggatgt cgatgtgcga ggcgagggat ttcggcttgc cggtatccag gcactcggcc
     4141 tcgaggaact cgtcgaagcg cgcggtgatg ccggcggcga cgcgatggag gatctcggtg
     4201 cgctcggcga cggtcatctt gccccacggg cccttcagtg cggcgcgcgc ggccttgacc
     4261 gcggcgtcga cctcggcctc gccggcttcg tggatcttgg cgatcacctg gccgttggcc
     4321 gggttgacgt tgtcgaacag cttgccgctg accgagccga cgtattcgcc gttgatgaag
     4381 tgtttgattt ctttcatggg cacgctcttg tcgaaggcaa tcaggtcagg acggtgagga
     4441 agcgctcgtt gagctggcgg tcgtggtaga agatcgcctt gcccaggtcg gcggcgttcc
     4501 aggtcaccgg cgggtggtcc ggatagtggt agtcaccacc gcagaacacc tcgttgcggt
     4561 tgccggacgg gtcgaagaag tagatggtct tgccgtgggt caggccgtgg cgggtcgggc
     4621 cgatgtcgat cgaggtgtcg gtcatgctga tcaggtcggc ggcgcgcagc acatcgtccc
     4681 aggtctcgag gaagaacgag gcgtggtgga acttgccctt ctcggcgtgc tggatgaacg
     4741 ccacgtcgtg ggccttcatg ctgcaggtga ggaactgggc gacgcgggtg ccttcggggt
     4801 cgagcacctg ctcggccaga tcgaagccga gcacctcggt gaacaggcgc agggtttccg
     4861 ccagctcgtc gccatacagc agggcgtggt cgaagcgggt cgccttgata cccttgaggc
     4921 cacgcggcca ggcttccggg ttgatctcgg cgatgcccca cttgccggtc tgctgcttct
     4981 cggcgaacag ctcgaagcag tggccggagg gcgcgctgaa gcggatgcgc cggccgcagc
     5041 ccttgagctc gtcggcgggg atgctctcca cggcgcaacc gaaggccacg agatcctcgg
     5101 tcaactggcc gagggcggct tcgttgatca ccttgaaacc catgaagtcc atgcctgcct
     5161 catcggcctc gcgcagcacc acggagaact tgtcgacttc ggtccagccc ttgaggtaaa
     5221 cgcggccctt gtcgtcgcgg tcgacctcga tcaggccgag cagctcgacg tagtgttcca
     5281 gggccttggc catgtccagt acgcgcagct ggatgtgacc gggacgcatc acgccttttt
     5341 tcatttcggc acctcttgta tttatcgttg ttgtttaaaa actggttggc tgtttagggg
     5401 cgcggccctc aatcgtgagg tcgctcagcg gatacagccg gcaggccagc acctcccccg
     5461 gcagcgtgcg cagcggccgg ggcatggcgg ctgctcattg gctcgcagtg atattcaccg
     5521 tgcaacacgc gcactttgca caggccgcaa ccgccgctac gtcagccaac cggcagacaa
     5581 cgccgccctc ttcgctccat ggcatgcaac acagaatgat cacctgggca cggaaagcgg
     5641 ataccactgc attgctcgtt aatctcgaaa atactgttca tccataatcc cggcgtttgt
     5701 gcgcacctct cacagctctt gaatggcgcg caactgaact cgttacaaga aatggatcga
     5761 agcacttgct caagcggcgc tgaagatgcg gtgcgggtcg atgacggccg gcgtcacacg
     5821 tcctgccaag gctccggcgt acggtcggcg acctgcagat aggcatcgcc tagacccagg
     5881 ccggcgatgg cgctcatcgc gttggggccg aaggacacgc cggcacccac ctcgccgaac
     5941 gccggcgcgg actcgaacag ctgtacctgg cgatgggaat ggcggcacag atccagtggc
     6001 aggctgacgc cgatgatgcc gatgggcaag gcaggtttgt tgttcatgct ggtacccgtg
     6061 atggttttgt tgtttgggta tgaccgatca tggcgaagcc agcactattg ataaatacac
     6121 caaacaccat acagactatt agagttgtga atgttcctgc cgacccggcg gagctttggc
     6181 gatgacccaa gcgaggttcc atggaactgc gtgatctgga gttgaagctg ctggtggtgt
     6241 tcaaccagtt gctggtggat cgacgcgtcg aattcgccgt agcacttggg cgcctcgtcg
     6301 aggccgattc acctgcacgc cgacgatgtc ggcgatgttc aggcgatccc acatgatcgc
     6361 ctgcatcagt tgacggttgt acttcatcac cgggggctgg ccggtgtgac ggagttgtac
     6421 ttttcgacca tctggcgagc ctctttgagc aaccctatgc cgttggcgta tccctgcttc
     6481 ccggcctcac ttacccagtc tccgtctaga ctgcgagcga gctccatcca ccgattc
//
LOCUS       DATCUN010000103         4224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_135113, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000103 DATCUN010000000
VERSION     DATCUN010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135113"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VIO83_03930"
     CDS             <1..884
                     /locus_tag="VIO83_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination factor protein RarA"
                     /protein_id="HEY8330457.1"
                     /translation="NALLSRARVYVLKSLDEAALRQLLARALSEERGLGKRRLTISEE
                     ASSLLLAAADGDGRRLLNFLENAADLVEDGEEIGVSLLENLLGDSRRRFDKGGEAFYD
                     QISALHKSVRGSNPDAALYWFARMLDGGCDPLYLARRVVRMASEDIGNADPRALNLCL
                     AAWEVQERLGSPEGELAIAQAIVYLACAPKSNAVYMGFKAAMRDAGAHGSLEVPLHLR
                     NAPTKLMKTLGYGDEYRYAHDEPEAYAAGEDYFPEGLEPQRYYQPVPRGLELKIREKL
                     EHLAALDRNSPRRRRKS"
     gene            881..1255
                     /gene="crcB"
                     /locus_tag="VIO83_03935"
     CDS             881..1255
                     /gene="crcB"
                     /locus_tag="VIO83_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEY8330458.1"
                     /translation="MIALFVAVAMGGAAGTLLRFVTSNWISAHWPQHFYTATLAVNLV
                     GCLLIGYLYGMFLIRPEVPLLLRAGLIVGFLGGLTTFSSFSLDTLRLLESGQAPLALI
                     YATASVLGGLLATWAGLSLTKL"
     gene            1272..2552
                     /gene="serS"
                     /locus_tag="VIO83_03940"
     CDS             1272..2552
                     /gene="serS"
                     /locus_tag="VIO83_03940"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY8330459.1"
                     /translation="MLDSKLVRGQPQEVAQRLATRGFQLDVARLEALESRRKETQTRT
                     EQLQAERNSRSKSIGQAKQRGEDIAPLMADVERMGGELAAGKQELETIQAELDALLLT
                     IPNLPHESVPVGGSEEENVEVSRWGVPREFDFEIKDHVALGEQHGWLDFETAAKLSGA
                     RFALMRGPIARLHRALAQFMLNLHVGEHGYEEAYTPYLVQAPALLGTGQLPKFEEDLF
                     KISREGEADFYLIPTAEVSLTNIVSGEILEAKQLPLKFVAHTPCFRSEAGASGRDTRG
                     MIRQHQFDKVEMVQIVEPAKSFEALEELTGHAEKVLQLLELPYRKLALCTGDMGFGAT
                     KTYDLEVWVPSQDKYREISSCSNCGDFQARRMQARWRNPETGKPELVHTLNGSGLAVG
                     RTLVAVLENYQQADGSIRVPEVLKPYMGGLEIIG"
     gene            2661..4052
                     /gene="cysG"
                     /locus_tag="VIO83_03945"
     CDS             2661..4052
                     /gene="cysG"
                     /locus_tag="VIO83_03945"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HEY8330460.1"
                     /translation="MDFLPLFHNLRGRRVLVVGGGEIALRKARLLAEAGATLRVVAPR
                     IEPGLRSLVEQGGGELILRGYREADLYGCVLVIAATDDDQLNARVSGDAYARNLPVNV
                     VDAPELCSVIFPAIVDRSPLMVAVSSGGDAPVLTRLMRARLETLIPSAYGRLAGLAQK
                     FRGAVKQRFADVQQRRVFWEEVFQGPVAEQVLAGREAEAERLLADKLAGSRERELGEV
                     YLVGAGPGDPDLLTFRALRLMQQADVVLYDRLVAPAIVDMCRRDAERIYVGKRRSEHA
                     LPQEQINQQLVVLAKQGKRVLRLKGGDPFIFGRGGEEIEELAAEGIPFQVVPGITAAS
                     GCSAYAGIPLTHRDYAQSVRFVTGHLKDNSCNLPWGDLVAPGQTLVFYMGLVGLPVIC
                     EQLIRHGCAADMPAALVQQGTTPSQRVFTGTLADLPQLVAQHEVHAPTLVIVGEVVRL
                     RDKLAWFEGASQA"
BASE COUNT          714 a         1230 c         1462 g          818 t
ORIGIN      
        1 acaatgcgct gctctcaagg gcgcgggtct atgtgctgaa gagcctcgat gaggcggcgc
       61 tgcgccagtt gctggcgcga gccctgagcg aagagcgcgg gctgggcaag cggcgtctga
      121 ccatcagcga ggaggccagc agtctgctgc tggcggcagc cgacggtgat ggccggcggc
      181 tgctcaattt tctggagaat gccgccgatc tggtggagga cggcgaagaa atcggtgtca
      241 gtctgctaga gaacctgctg ggcgacagcc gccggcgctt cgacaaggga ggcgaggcct
      301 tctacgacca gatcagcgcg ctgcacaagt cggtgcgtgg ctccaatccg gatgccgccc
      361 tgtactggtt cgcgcgcatg ctcgacggcg gctgtgaccc gctgtacctc gcccgccgcg
      421 tagtgcgcat ggccagcgag gatatcggca acgccgaccc gcgggcgctc aacctgtgtc
      481 tggccgcgtg ggaggtgcag gagcgtctcg gcagtcccga gggcgagctg gcgatcgcgc
      541 aggcgatcgt ttatctggcc tgcgcgccga agagcaatgc ggtgtacatg ggcttcaaag
      601 cggcgatgcg cgatgccggg gcccatgggt cgctggaggt gccgctgcac ctgcgtaacg
      661 cgccgaccaa gctgatgaag acgctgggct acggcgacga ataccgctat gcccacgacg
      721 agccggaggc ctacgcggct ggggaggatt acttccccga ggggctggag ccgcagcgct
      781 actaccagcc ggtgccgcgg gggctggagc tgaagatccg cgaaaagctc gagcatctgg
      841 ctgctcttga tcgcaacagc ccgcggcggc ggagaaagtc atgatcgccc tgtttgtcgc
      901 cgttgccatg ggcggcgcgg cgggtacctt gctgcgtttt gtcaccagca actggatcag
      961 cgcccactgg ccacagcatt tctataccgc caccctggcg gtaaatcttg tcggttgcct
     1021 gctgatcggt tatctctacg gcatgttttt gattcgtccg gaagtgccgc tgttgctgcg
     1081 cgccgggctg atcgtcggct ttctcggtgg cctgaccacc ttttcatcct tctccctcga
     1141 cacgctacgc ctgctggaaa gcggccaagc accgctggcg ctcatctatg cgactgccag
     1201 tgtgctgggc ggcttgcttg cgacttgggc ggggctgtct ttaaccaaac tctgattgac
     1261 gagaattctc catgctcgac tccaaactgg ttcgtggcca accgcaggaa gtggcgcagc
     1321 gtctggctac ccgcggtttt caactggatg tggcgcgtct cgaggcgctg gaaagccggc
     1381 gcaaggaaac ccagacccgc accgagcagc tgcaagccga gcgtaattcc cgctccaaga
     1441 gcatcggcca ggccaagcag cgtggcgaag acatcgcgcc cttgatggcc gatgtcgagc
     1501 gcatgggtgg cgagctggcg gcgggcaagc aggagctgga gacgatccag gccgagttgg
     1561 atgcgttgct gctgaccatt ccgaatctac cgcatgagtc ggtgccggtc ggcggcagtg
     1621 aggaggagaa cgtcgaggtg agccgctggg gagtcccgcg cgagttcgac ttcgagatca
     1681 aggaccacgt cgctcttggc gaacagcacg gctggctcga cttcgagacc gcggccaagc
     1741 tctccggcgc gcgtttcgcg ctgatgcgcg gcccgatcgc ccgtttgcat cgcgccctgg
     1801 cgcagttcat gctcaacctg cacgtcggtg agcacggtta cgaggaggcc tatacgcctt
     1861 atctggtcca ggcgccggcg ctgctgggta ctggtcagct gccgaagttc gaggaagatc
     1921 tgttcaagat cagccgggaa ggtgaagcag acttctacct gatccccact gcggaagtgt
     1981 cgctgaccaa catcgtctcc ggcgagatcc tcgaggccaa gcagctgccg ctgaaattcg
     2041 tcgcccacac cccatgcttc cgcagcgaag ccggggcttc tggtcgcgac acccgcggca
     2101 tgatccgcca gcaccagttc gacaaggtcg aaatggtgca gatcgtcgag ccggcgaagt
     2161 ccttcgaggc cctcgaggag ctgaccggcc atgccgaaaa ggtgctgcag ctgctcgagc
     2221 tgccgtatcg caagctggcg ctgtgcactg gcgacatggg cttcggtgcg accaagacct
     2281 atgacctgga ggtctgggtg ccgagccagg acaagtaccg cgagatttcc tcctgctcca
     2341 actgcggcga cttccaggcg cgtcgcatgc aggcgcgctg gcgcaatccg gaaaccggca
     2401 agccggagct ggtgcacacc ctgaacggtt ccggcttggc ggtcggccgt actctggtgg
     2461 cggtactgga aaactatcag caggctgatg gctccattcg tgtgccagag gtgctcaagc
     2521 cgtatatggg tgggctcgag attatcggct gactgggccg gcaatccacc gagggcttgt
     2581 cgccggcagg ccctctccgc gcgatgacgg ttgccccgtc accctgccac aagcaacgtg
     2641 gcgactgtca gaggcttgcg atggactttc ttccgctctt ccacaatctg cgtggccgcc
     2701 gggtgttggt ggtcggcgga ggtgagatcg ctttacgcaa ggcgcggttg ctggccgagg
     2761 cgggtgccac gcttcgggtg gtggcgccca ggatcgagcc cggcctgcgc agcctggtgg
     2821 aacagggcgg cggcgagctg atcttgcgcg gctatcggga ggcggatctg tacggctgcg
     2881 tgctggtgat tgcagctacc gatgacgatc agctcaatgc ccgggtgtcc ggcgatgcct
     2941 atgcccgcaa tctgccggtc aatgtggtgg atgcgccgga gctctgcagt gtgatttttc
     3001 cagccatcgt cgaccgctcg ccgctgatgg tggcggtttc cagcggtggc gatgcgccgg
     3061 tgttgacccg tctgatgcgt gcccgcctgg agacgttgat tccgtcggct tacggtcgcc
     3121 tggctgggtt ggcacagaag ttccgtgggg cggtaaagca gcgcttcgct gatgtgcagc
     3181 agcgccgggt gttctgggaa gaggttttcc aggggccggt cgcggagcaa gtgttggcgg
     3241 gtcgcgaggc ggaagctgag cgcttgttgg ccgacaagct ggcaggtagc cgtgagcgag
     3301 agctgggcga ggtttatctg gttggtgcgg gcccgggcga tccggatctg ctcacttttc
     3361 gggccttgcg cctgatgcag caggctgacg tggtgcttta tgaccgactg gttgccccgg
     3421 cgatcgtcga catgtgccgg cgcgatgccg agcgcatcta tgtcggcaag cgccgcagcg
     3481 aacatgcctt gccgcaagaa cagatcaacc agcaattggt ggtgctggcc aagcagggca
     3541 agcgggtgct gcgcctgaag ggcggcgacc cgttcatctt cggtcgtggt ggcgaggaga
     3601 tcgaggagtt ggctgcggaa ggcatcccct tccaggtggt gcccggcatt accgccgcct
     3661 ccggttgctc ggcctatgcg ggcattcctt tgacccaccg cgattacgcg caatcggtgc
     3721 gcttcgtgac cggacacctg aaggacaata gctgcaacct gccatggggt gatcttgttg
     3781 cgccggggca gaccctggtg ttctacatgg ggctggttgg cctgccggtg atctgtgagc
     3841 agttgatccg tcacggctgt gcggcggata tgccggcagc gctggtgcag cagggaacca
     3901 cgcccagcca gcgagtgttc actggtactc tggcggatct gccgcagctg gtggctcagc
     3961 acgaagtgca tgcgccgaca ctggtgatag taggagaggt ggtccggttg cgggacaagc
     4021 tggcctggtt cgagggggca agccaagcct gaatggcgat gtttgtgaga gggtgttgac
     4081 agcgttttta aaggctgtat tattcgccac ctcgctgcgg cggcctccgg gttgaagcta
     4141 gcgcaagtgg ttgaaacgaa aagaaatttt cacgaaacgc ttgacaggtt gtaaggctgc
     4201 tgtagaattc gcggcctcgg ttga
//
LOCUS       DATCUN010000104         3884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_4196, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000104 DATCUN010000000
VERSION     DATCUN010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4196"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VIO83_03950"
     CDS             <1..298
                     /locus_tag="VIO83_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY8330461.1"
                     /translation="LLCLPRGDGHVDLRRLLLELAGGGANEVLVEAGPRLAGAFAREG
                     LVDEYRIFMAPQLLGSSARPLLDWPLQRMAEAPQLAIREIRAVGRDWQIIAVPT"
     regulatory      423..574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            696..1358
                     /locus_tag="VIO83_03955"
     CDS             696..1358
                     /locus_tag="VIO83_03955"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY8330462.1"
                     /translation="MFTGIIEAIGSIRAMTPKGGDVRVYVATGKLDLGDVKLGDSIAV
                     NGTCLTAVELPGDGFWADVSRETLTRTAFGDLKVGSPVNLEKALTPTTRLGGHLVSGH
                     VDGVGEIVSRADNARAIQFRVRAPRELAKYIALKGSITVDGVSLTVNAVDGADFELTI
                     VPHTLQETIMADYRSGRRVNLEVDLLARYLERLLLGDKAAEPAGHGISEAFLAEHGFL
                     KG"
     gene            1395..2486
                     /gene="ribBA"
                     /locus_tag="VIO83_03960"
     CDS             1395..2486
                     /gene="ribBA"
                     /locus_tag="VIO83_03960"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEY8330463.1"
                     /translation="MALNKIAELVEDIRQGKMVILMDDEDRENEGDLIMAAECVRAED
                     INFMAKHARGLICMPMDRARCERLGLPLMVQRNGSGFGTKFTVSIEAAEGVTTGISAA
                     DRARTVQAAAALEAKAEDIVSPGHIFPLMAQPGGVLARAGHTEAACDLARMAGFQASG
                     VICEIMNDDGSMARRPELEQFAEEHGIKIGTIADLIHYRLVHERTVERVSERQLDSEL
                     GQFKLFTYRDAVEGQVHLALIKGEIKADEPTLVRVHNMDPLRDLLSVKRPGRWSLREA
                     MLKVAEQGSGVVLLLNHAMSGNELLALLEEQLGGAKPAEPTTYSTVGAGSQILRDLGV
                     RKMRLLSSPVRFNAISGFDLEVVEYVTCE"
     gene            2549..3025
                     /gene="ribE"
                     /locus_tag="VIO83_03965"
     CDS             2549..3025
                     /gene="ribE"
                     /locus_tag="VIO83_03965"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY8330464.1"
                     /translation="MTLKTIEGTFIAPKGRYALVVGRFNSFVVESLVQGAIDALVRHG
                     VSESELTIIRAPGAFEIPLVAQKVAQRGEFDAIIALGAVIRGGTPHFEYVAGECTKGL
                     AQVSLQFGVPVAFGVLTVDSIEQAIERSGTKAGNKGAEAALSALEMVSLLAQLEAK"
     gene            3022..3504
                     /gene="nusB"
                     /locus_tag="VIO83_03970"
     CDS             3022..3504
                     /gene="nusB"
                     /locus_tag="VIO83_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY8330465.1"
                     /translation="MSEHDSGTPAPKAARPNKIAMRQKARSLAVQALYSWQLAGQSIN
                     EIEAQFRVDNDFSDVDGAYFREILHGVPRLKQELDAAFGPCLDRPVEELDPVELAILR
                     LSTYELLNRADVPYRVVINEGIELAKVYGATDGHKFVNGVLDKLAPRLRGAEVRNKRS
                     "
     gene            3515..>3884
                     /locus_tag="VIO83_03975"
     CDS             3515..>3884
                     /locus_tag="VIO83_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEY8330466.1"
                     /translation="MAGLGEFELIRQYFAAAVCATPACGVARGIGDDCALLQLLPGEQ
                     LAVSTDTQVAGVHFPHSHDSFLLAQRVLAAAASDLAAMGAAPLGFTLALTLPAADPAW
                     LEGFARGLDVMARGCRLALIG"
BASE COUNT          638 a         1361 c         1283 g          602 t
ORIGIN      
        1 gttgctgtgt ctgccgcgcg gcgacgggca tgtcgacctg cgccgcctgc tcctcgagct
       61 cgctggcggc ggtgccaacg aggtgctggt ggaggcaggc ccgcgcctgg ctggcgcctt
      121 cgcccgcgag gggctggtgg acgaataccg gatcttcatg gcgccgcagt tgctcggctc
      181 cagcgcccgg ccgctgctcg actggccact gcagcgcatg gccgaggcgc cgcagctggc
      241 gatccgcgag atccgcgcgg tcggtcgcga ctggcagatc atcgcggtac cgacgtaggg
      301 ccacgggcgg tgcgcgtggc gcaccctacg cgtggggcgc cctgcgggta gggtgcgccg
      361 tgcgcaccaa gcgccatccg cgcaaaatat ggtacaaagt cccacgcggt cgccaacggc
      421 cgcgacgttc tcagggcggg gtgcaattcc ccaccggcgg tgatggcgca gcctgcgccc
      481 agcccgcgag cgcttgccac cttgggcggc aaggtcagca gatccggtgc gattccgggg
      541 ccgacggtta cagtccggat gagagagagc gggattcctt cctcggggcg gcccatccgt
      601 gcccgcgaaa tccccttcga tccaacgccc tgttttatcc aaaacaggag ttcgaccatg
      661 caagtgaata ccctcttcgc gctgcgggag gcctgatgtt taccggaatc atcgaagcca
      721 tcggcagcat tcgcgccatg acccccaagg gcggtgacgt gcgcgtctac gtcgccaccg
      781 gcaagctcga cctcggcgac gtcaagctcg gcgacagcat cgccgttaac ggcacctgcc
      841 tgaccgcggt ggagctgccg ggcgacggtt tctgggccga cgtcagccgc gagaccctga
      901 cccgcaccgc cttcggcgac ctcaaggtcg gcagcccggt caacctggag aaggcgctga
      961 cgccgaccac ccgcctcggc ggccacctgg tcagcggcca cgtcgacggc gtcggcgaga
     1021 tcgtctcgcg cgccgacaat gcccgcgcca tccagtttcg cgtgcgtgca ccgcgcgaac
     1081 tggccaagta cattgctctc aagggctcga tcaccgtcga tggcgtcagc ctgacggtga
     1141 acgcggtgga tggcgccgat ttcgagctga ccatcgtccc gcacaccctg caggaaacca
     1201 tcatggccga ctaccgcagc ggccgccggg tcaacctcga ggtcgacctg ctggcgcgct
     1261 acctggagcg cctgttgctt ggcgacaagg ctgccgagcc ggccggtcac ggcatcagcg
     1321 aggcgttcct cgccgaacac ggcttcctca agggctgagc cgcttttcca tcacgaattc
     1381 acaagggggt gcctgtggcg ctcaacaaga ttgccgagct ggtcgaggac attcgccagg
     1441 gcaagatggt cattctcatg gatgacgagg atcgcgagaa cgaaggcgac ctgatcatgg
     1501 ccgccgagtg cgtgcgcgcc gaggacatca acttcatggc caagcatgcg cgcggcctga
     1561 tctgcatgcc gatggaccgc gcgcgctgcg agcgcctcgg cctgccgctg atggtgcagc
     1621 gcaacggctc cggcttcggc accaagttca ccgtctccat cgaggcggcc gagggggtga
     1681 ccaccggcat ctccgccgcc gaccgcgcgc gcaccgtaca ggccgccgcg gcgctggagg
     1741 ccaaggccga ggacatcgtc agcccgggcc acatcttccc gctgatggcc cagcccggcg
     1801 gcgtgctggc gcgtgccgga cacaccgagg cggcctgcga cctggcgcgc atggccggct
     1861 tccaggccag cggggtgatc tgcgagatca tgaacgacga cggcagcatg gcccgtcgcc
     1921 cggagctgga acagttcgcc gaagagcatg gcatcaagat cggcaccatc gccgacctga
     1981 tccactaccg cctggtccac gagcgcaccg tcgagcgcgt ctccgagcgc cagctggaca
     2041 gcgagctggg ccagttcaag ctgtttacct accgcgacgc ggtcgagggc caggtgcacc
     2101 tggcgctgat caagggcgag atcaaggccg acgagccgac cctggtgcgc gtgcacaaca
     2161 tggacccgct gcgcgacctg ctcagcgtca agcgcccggg gcgctggagc ctgcgcgagg
     2221 cgatgctcaa ggtggccgag cagggcagcg gcgtagtcct gctgctcaac catgcgatga
     2281 gcggcaacga gctgctggcc ctgctcgagg agcagctcgg cggtgccaag ccggccgagc
     2341 cgaccaccta cagcaccgtc ggtgccggct cgcagatcct ccgcgatctg ggtgtgcgca
     2401 agatgcgcct gctcagctcg ccggtcaggt tcaacgcgat atccggcttc gatctggaag
     2461 ttgtagaata cgtcacctgc gaataacgct atctgtgaat aacacctcgg ggcgcactgg
     2521 cgccccggct ctttatctgg aatccgtcat gaccctgaag accatcgaag gtaccttcat
     2581 cgcccccaaa ggccgttacg ccctggtggt tggccgcttc aacagcttcg tcgtcgaaag
     2641 cctggtacag ggcgccatcg acgccctggt gcgccacggc gtcagcgaga gcgagctgac
     2701 catcatccgc gccccgggcg ccttcgagat tccgctggtt gcccagaaag tcgcccagcg
     2761 cggcgaattc gacgccatca tcgcccttgg cgcggtgatc cgcggcggta ctccgcactt
     2821 cgaatacgtc gccggcgagt gcaccaaggg cctggcccag gtgtccctgc agttcggcgt
     2881 gccggtcgcc ttcggcgtgc tgaccgtcga ctccatcgag caggccatcg agcgctccgg
     2941 caccaaggcc ggcaacaagg gcgccgaagc cgccctgtcc gccctggaaa tggtcagcct
     3001 gctggcgcag ttggaggcca agtgagcgag cacgatagcg gcactccggc gcccaaggca
     3061 gcccgaccga acaagatcgc catgcgccag aaggcccgca gcctggcagt gcaggccctc
     3121 tactcgtggc agctggccgg tcagtcgatc aacgagatcg aagcgcagtt ccgcgtcgac
     3181 aacgacttca gcgatgtcga cggcgcctac ttccgcgaga tcctgcacgg cgtaccgcgc
     3241 ctgaagcagg agctggatgc cgccttcggg ccctgtctcg accgcccggt ggaagaactc
     3301 gatccggtcg agctggcgat cctgcgcctg tcgacctacg agctgctcaa ccgcgccgac
     3361 gtaccctatc gtgtggtgat caacgaaggc atcgagctgg ccaaggtcta cggcgccacc
     3421 gacggccaca agttcgtcaa cggcgtgctg gacaagctgg ccccgcgcct gcgcggcgcc
     3481 gaagtacgca acaagcgcag ctgatccccg cgcgatggcc ggtctcggcg aattcgagct
     3541 gatccgtcag tacttcgccg cggccgtctg cgccacgccg gcttgcggcg tggcccgcgg
     3601 catcggtgac gactgtgcgc tgctgcaact gctgcccggc gaacaactgg cggtgtcgac
     3661 cgacacccag gtcgccgggg tgcacttccc gcattcccac gactccttcc tgctcgccca
     3721 gcgcgtgctg gccgccgcgg ccagtgacct ggcggcgatg ggcgcggcgc cgctcggctt
     3781 caccctggcg ctgaccctgc ccgccgccga tccggcctgg ctggagggct tcgcccgcgg
     3841 cctcgacgtg atggcccgcg gctgccgcct ggccctgatc ggcg
//
LOCUS       DATCUN010000105         6742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_79222, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000105 DATCUN010000000
VERSION     DATCUN010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79222"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VIO83_03980"
     CDS             <1..999
                     /locus_tag="VIO83_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEY8330467.1"
                     /translation="GLEPLTLVAKEGLALLNGTQVSSAYALRGLFEAEDLLAAATVCG
                     SLSVEAALGSRVPFDARIHAVRGQRGQIDMATAYRHLLGAGSAIAASHAACGKVQDPY
                     SLRCQPQVMGACLTQLRQAAEVLATEANAVSDNPLVFAADDAVLSGGNFHAEPVAMAA
                     DNLALAIAEIGALAERRIALLIDHNLSQLPAFLVDSGGVNSGFMIAQVSAAALASANK
                     ALAHPHSVDSLPTSANQEDHVSMAPNAGMRLWEMAANVRGILAVEWLAACQGLDFRRG
                     LHSSPPLERARRLLRERVPFYAHDRFFAPDIEAADALLRERCLSGLLPAGLLPSLG"
     gene            1162..1347
                     /locus_tag="VIO83_03985"
                     /pseudo
     CDS             1162..1347
                     /locus_tag="VIO83_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339765.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1323..1949)
                     /locus_tag="VIO83_03990"
     CDS             complement(1323..1949)
                     /locus_tag="VIO83_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HEY8330468.1"
                     /translation="MLFKKLLLVSISLLVAFVAYANLKVIRYQDFSGNVVGELPTEKA
                     CLVLGTSKYLKSGGENLFYKFRIEAARQVYSAGKCSRIVVSGDNRHHDYNEPEEMRKS
                     LVEQGVPVADIYCDYAGGRTLDSVVRFKHVFGQASGIVISQEFHNARAIYIGKANGVN
                     LTGFNAREVGAYSGLRTKVREVFSRVRAVVDVEVLHSEPRHYGEPIQL"
     gene            complement(2050..2997)
                     /locus_tag="VIO83_03995"
     CDS             complement(2050..2997)
                     /locus_tag="VIO83_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8330469.1"
                     /translation="MNLESKWLEDFVALAATRSFSQAAQKRFVTQPAFSRRIRTLEEA
                     LGLTLVNRARTPVELTEAGQLFLVTARSVVEQLGEAVRHLHNLEGEQGEVLQFAAAHS
                     LTLGFFPAWIAGLRRAGLPIKSRLVATNVGEAVHALRDGTCDLILAYYDPDAALQLDP
                     ELFPSLHLGDTAMLPVCAVDAAGQPLFDLDSGQNVPLLAYSAGAFLGRSVNLLLRQRG
                     LRATTVYETAMADSLKSMALQGLGVAWVPRLSVTAELARGELVVCGGEHWQVPLEIRL
                     HRCALLHKPAVRRLWKVLEEGAGVLAGAGSDAGPVRQGG"
     gene            3169..4593
                     /gene="aspA"
                     /locus_tag="VIO83_04000"
     CDS             3169..4593
                     /gene="aspA"
                     /locus_tag="VIO83_04000"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008078790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEY8330470.1"
                     /translation="MSAAASFRIEKDLLGTLEVPADAYYGIQTLRAVHNFRLSGVPLA
                     HYPKLVVALALVKQAAADANLELGHLSEAKHAVISQACARLIRGEFHEQFVVDMIQGG
                     AGTSTNMNANEVIANLALEAMGHAKGEYQYLHPNNDVNMAQSTNDAYPTAIRLGLLLG
                     HDALLASLDSLIQAFAAKGEEFAEVLKMGRTQLQDAVPMTLGQEFRAFATTLGEDLDR
                     LKRLVPELLTEVNLGGTAIGTGINADPRYQQLAVTRLAAISGQPLVPAADLIEATYDM
                     GAFVLLSGMLKRTAVKLSKICNDLRLLSSGPRTGINEINLPARQPGSSIMPGKVNPVI
                     PEAVNQVAFTVIGNDLAVTMAAEAGQLQLNVMEPLIAYKTLDSIRLLQRAMDMLREHC
                     VVGITANVERCRQLVEQSIGLVTALNPYIGYENATRIAKLALDSGRGVLELVREEGLL
                     DEDLLADILRPENMIAPRRVPLQG"
     gene            complement(4691..4906)
                     /locus_tag="VIO83_04005"
     CDS             complement(4691..4906)
                     /locus_tag="VIO83_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HEY8330471.1"
                     /translation="MSDHHVYKKIELVGSSRTSIEDAINNALAEAAKTVHNLDWFEVV
                     ETRGHIANGKVGHWQVTVKIGFRIVGS"
     gene            complement(4971..6008)
                     /locus_tag="VIO83_04010"
     CDS             complement(4971..6008)
                     /locus_tag="VIO83_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEY8330472.1"
                     /translation="MSILRLLPLLIVLTGCSSLGYYAQLGEGQWQLLDARRPVAQLLA
                     DPQQPAALRQRLRLSQEARDFASRQLGLPDNRSYRLYADLKRPYVVWNLFATAEFSLE
                     PLTHCFPIAGCVAYRGYFDEQRARGAAALLRVQGLDTEVSGVEAYSTLGWFDDPLLNS
                     MLTWSDARLAGLIFHELAHQRLYLPGDTAFNESFARFVEEEGLRQWRHARGLPPPPLH
                     LARQREQFVELVLGSRQRLETLYAQPLPVAVMRAAKQAEFQRLRRDYQALRDGPWHGD
                     GRFDGWFARPLNNARLLPFGLYEQWVPAFAALFAEAGRDWARFYARVGELARLSEEQR
                     RQALAALQPRG"
     gene            6095..6508
                     /locus_tag="VIO83_04015"
     CDS             6095..6508
                     /locus_tag="VIO83_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015474856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330473.1"
                     /translation="MRALPLLVVLALLGACAGPMPTPDPQQAWIELRSPPTELLMAER
                     LDDVRLNDGRYFQVSPGAHRLLATYRFEVSVGPIMTLEPVQMLCYLRLDYDNFVAGQR
                     YRLEARSWLFQTESWLSDASGKRLADGQEWHCITQ"
     gene            complement(6563..>6742)
                     /locus_tag="VIO83_04020"
     CDS             complement(6563..>6742)
                     /locus_tag="VIO83_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8330474.1"
                     /translation="VAIAPQLLVADDLAAGRLEAPWGFVETPARLALWVPARGADRRA
                     VELAEWLRAELAANL"
BASE COUNT         1119 a         2379 c         2184 g         1060 t
ORIGIN      
        1 ggtctcgagc cgctgaccct ggtcgccaag gagggcctgg ccctgctcaa cggcacccag
       61 gtgtccagcg cctacgccct gcgcggcctg ttcgaggccg aggacctgct ggccgccgcc
      121 accgtgtgcg gcagcctcag cgtcgaggcc gcgctcggct cgcgggtgcc cttcgatgcg
      181 cgcatccacg ccgtgcgcgg ccagcgcggg cagatcgaca tggccaccgc ctaccgccac
      241 ctgctcggcg ccggcagcgc gatcgccgcc tcccacgccg cctgcggcaa ggtgcaggac
      301 ccctactcgc tgcgctgcca gccgcaggtg atgggcgcct gcctgaccca gctgcgccag
      361 gccgccgagg tgctggcgac ggaggccaac gcggtgtcgg acaacccgct ggtgttcgcc
      421 gccgacgacg cggtgctctc cggcggcaac ttccacgccg agccggtggc catggccgcc
      481 gacaacctgg cgctggccat cgccgagatc ggcgcgctgg ccgagcggcg catcgccctg
      541 ctgatcgacc acaacctgtc gcagctgccg gcgttcctgg tcgacagcgg cggggtcaac
      601 tccggcttca tgatcgccca ggtcagcgcc gcggcgctgg ccagcgccaa caaggccctc
      661 gcccacccgc acagcgtcga cagcctgccg acctcggcca accaggaaga ccacgtgtcg
      721 atggcgccca acgccggcat gcgcctgtgg gagatggccg ccaacgtgcg cggcatcctc
      781 gccgtcgaat ggctggccgc ctgccagggc ctggattttc gccgcggcct gcacagctcg
      841 ccgccgctgg aacgcgcgcg ccgcctgctg cgcgagcggg tgccgttcta cgcccacgac
      901 cgcttcttcg cccccgacat cgaagccgcc gatgcgctgc tgcgcgaacg ctgcctgtcc
      961 gggctgttgc cggcggggtt gctgcccagc ctgggctgag cggcatgaat gcatgggcga
     1021 cggtcgccac cttcgcctgg agggaagcca tggcccggtg cgccgtgcgc accataaaag
     1081 actgcctgac caccggctgc ttccgccccc tccccacgag gtgctggccg accagtcgac
     1141 cgccgctggg catcgacacc gctgtcgatg atccgccgca cctgatcggg ggagaacctg
     1201 atcagctacg cgcggctgat tcatccgggg gataggtaca agctgcggcc gtcgatatgg
     1261 ggggtggggg tggccctact tgccgctgtg ggaaaaaccc atactggact tgagaaacgc
     1321 actcatagct gtattggctc cccataatgt ctcggctccg aatgaaggac ctctacatca
     1381 actactgccc tgactcttga aaatacctct cgcacctttg tgcgcaatcc actgtaagca
     1441 cccacttccc tggcattgaa gcctgtgaga ttgacaccat tggccttgcc tatgtagatg
     1501 gccctcgcat tgtggaactc ctgagaaatc acgatgcctg aagcttggcc aaagacatgc
     1561 ttgaatctca ctaccgagtc caaggttcta cctccagcat agtcacaata aatgtcggct
     1621 actggaaccc cctgttccac caggcttttc ctcatctctt caggttcgtt gtagtcatga
     1681 tgtcgattgt ctccagagac gacgattctg gagcacttgc ctgccgaata cacctgcctt
     1741 gcagcctcga tcctgaactt gtagaacagg ttctcgccgc cacttttcag atatttggaa
     1801 gttccaagca ccaagcacgc tttttctgtg ggaagctcac ctaccacatt gccggaaaaa
     1861 tcctgatacc ggatcacttt caggtttgca taggcaacaa aagccaccag cagcgaaata
     1921 gaaacaagca gtaatttctt gaagagcatc cctgtcgaac ctcatcctga tgtatataac
     1981 gaagcaagta gggtagacaa cggcgcagcc ttgtctaccg atgccagcgg tggctaatcg
     2041 ctgcgcgagt tacccgccct gccggaccgg gccggcatcg ctcccggcgc cagcaagaac
     2101 cccagcccct tcctccaaca ccttccacaa cctcctcacc gccggcttgt gcagcagcgc
     2161 gcagcggtgc aggcggattt ccagcggcac ctgccagtgc tcgccgccgc acaccaccag
     2221 ctcgccgcgc gccagctcgg cggtcaccga caggcgcggc acccaggcca cgccgaggcc
     2281 ctgcagggcc atgcttttca ggctgtcggc catcgccgtc tcgtataccg tggtggcgcg
     2341 caggccgcgc tggcgcagca gcaggttgac cgagcgaccg aggaaggcgc cggcgctgta
     2401 ggccagcaat ggcacgttct ggccgctgtc gagatcgaac agcggctggc cggcggcatc
     2461 caccgcgcat accggcagca tcgcggtgtc gcccaggtgc agcgagggga acagctcggg
     2521 gtcgagctgc agcgcggcgt ccgggtcgta gtaggcgagg atcaggtcgc aggtgccgtc
     2581 gcgcagggcg tgcaccgcct cgccgacgtt ggttgccacc aggcggctct tgatcggtag
     2641 gccggcgcgg cgcaggccgg cgatccatgc cgggaagaag ccgagggtca gcgagtgggc
     2701 ggcggcaaac tgcagtacct cgccctgttc gccttcgagg ttgtgcaggt ggcgcaccgc
     2761 ctcgccgagc tgctcgacca cgctgcgcgc ggtgaccagg aacagctggc cggcctcggt
     2821 cagctccacc ggggtgcgcg cgcggttgac cagggtcagg ccgagggcct cctccagcgt
     2881 gcggatgcgc cggctgaacg ccggctgggt gacgaagcgc ttttgcgccg cctgggaaaa
     2941 gctgcgggtg gcggccaggg cgacgaagtc ttccagccat ttgctttcca ggttcatcgc
     3001 gcctccgccg gccgctgccg gacacccggc gtcaagccgc cgttaccgtc tcattatgcc
     3061 gattcggcat ggcccagcaa gcaacagcat tggccgccga tccgccgcag cccttaatct
     3121 atgggcgtcg cggtgcttgc cgccattcct tcggaacctc acgccatcat gtccgctgct
     3181 gcatccttcc gcatcgaaaa agatttgctc ggcaccctcg aagtccccgc cgatgcctac
     3241 tacggcatcc agaccctgcg tgcggtgcac aacttccgcc tgtccggcgt gccgctggcg
     3301 cactacccga aactggtggt cgccctggcc ctggtcaagc aggccgccgc cgacgccaac
     3361 ctcgagctgg gccacctcag cgaggccaag cacgcggtga tcagccaggc ctgcgcgcga
     3421 ctgatccgcg gcgagttcca cgagcagttc gtggtcgaca tgatccaggg cggcgctggc
     3481 acctcgacca acatgaatgc caacgaggtg atcgccaacc tggcgctgga ggcgatgggc
     3541 cacgccaagg gcgagtacca gtacctgcac ccgaacaacg acgtgaacat ggcgcagtcg
     3601 accaacgacg cctatccgac cgccatccgc ctgggcctgc tgctcggtca cgacgccctg
     3661 ctggccagcc tcgacagcct gatccaggcc ttcgccgcca agggcgagga gttcgctgag
     3721 gtgctgaaga tgggccgcac ccagctgcag gatgccgtgc cgatgaccct cggccaggag
     3781 ttccgcgcct tcgccaccac cctcggcgag gacctggatc gcctcaagcg cctggtgccg
     3841 gagctgctca ccgaggtcaa cctcggcggc accgcgatcg gcaccgggat caacgccgac
     3901 ccgcgctacc agcagctggc ggtgacccgc ctggcggcga tcagcggcca gccgctggtg
     3961 ccggcggccg acctgatcga ggccacctac gacatgggcg ccttcgtgct gctctccggc
     4021 atgctcaagc gcaccgcggt caagctgtcg aagatctgca acgacctgcg cctgctgtcc
     4081 agcggcccgc gcaccgggat caacgagatc aacctgccgg cgcgccagcc gggcagctcg
     4141 atcatgccgg gcaaggtcaa tccggtgatc cccgaggcgg tcaaccaggt ggccttcacc
     4201 gtgatcggca acgacctggc ggtgaccatg gcggctgagg ccggccagct gcagctcaac
     4261 gtcatggagc cgctgatcgc ctacaagacg ctcgactcga tccgcctgct gcagcgcgcc
     4321 atggacatgc tgcgcgagca ctgcgtggtg ggcatcaccg ccaacgtcga gcgctgccgc
     4381 cagctggtcg agcagtccat cggcctggtc actgcgctca acccctacat cggctacgag
     4441 aacgccacgc gcatcgccaa gctggcgctg gacagcggcc gcggcgtgct agagctggtg
     4501 cgcgaggagg gtctgctcga cgaggacctg ctggccgaca tcctccgtcc ggaaaacatg
     4561 atcgcgccgc ggcgggtgcc gctgcagggc tgagcctgcg gatgaccggc gtcactggcg
     4621 ccggcgtggc agggacgctg gtctgcctag aggcgatcga cgggcacgag ttgcccgcga
     4681 tcgcctcggc tcaactgccg acgatgcgga agccgatctt caccgtcacc tgccagtggc
     4741 cgaccttgcc gttggcgata tgcccgcggg tttccaccac ctcgaaccag tccaggttgt
     4801 gcacggtctt ggctgcttcg gccagggcgt tgttgatcgc gtcctcgatg ctggtgcgcg
     4861 aggagccgac cagctcgatc ttcttgtaga cgtggtgatc cgacatgacg gcgctccttg
     4921 gtgcgcaggg ggtctcctga acgctagtca aggtgcgccc agccggcaaa tcagccgcgc
     4981 ggctgcaacg cggccagtgc ctgacggcgc tgctcctccg acaggcgggc cagctcgccc
     5041 acccgcgcat agaagcgcgc ccagtcgcgg ccggcctcgg cgaacagcgc ggcgaaggcc
     5101 ggcacccact gctcgtagag gccgaacggc agcaggcggg cattgttcag cggacgggcg
     5161 aaccagccgt cgaaacgccc gtcaccgtgc cagggtccgt cgcgcagggc ctggtagtcg
     5221 cggcgcagac gctggaactc ggcctgcttg gcggcgcgca tcaccgccac cggcagcggc
     5281 tgggcgtaca gcgtttccag gcgctggcgg ctgcccagca ccagctcgac gaactgctcg
     5341 cgctggcgcg ccagatgcaa tggtggtggc ggcaggccgc gcgcgtggcg ccactggcgc
     5401 aggccctcct cctcgacgaa gcgggcgaag gattcgttga acgcggtgtc gccaggcagg
     5461 tacaggcgct ggtgggccag ctcgtggaag atcagccccg ccagccgcgc gtcgctccag
     5521 gtcagcatgc tgttcagcag cggatcgtcg aaccagccga gggtggaata ggcctcgacc
     5581 ccggacacct cggtgtccag gccctgcaca cgcagcagcg cggcggcgcc gcgggcgcgc
     5641 tgctcgtcga agtagccgcg gtaggccacg cagccggcga tggggaagca gtgggtcagc
     5701 ggctccaggg aaaactccgc ggtggcgaac aggttccaca ccacgtaggg ccgcttgagg
     5761 tcggcgtaca ggcggtagct gcggttgtcc ggcaggccca gctggcggct cgcgaagtcg
     5821 cgcgcctcct ggcttaggcg caggcgctgg cgcagtgctg ccggttgctg cgggtcggcg
     5881 agcagctgcg ccaccggccg gcgcgcatcg agcagctgcc actggccctc gcccagctgg
     5941 gcgtaatagc cgaggctgga gcagccggtg agtacaatga ggagcgggag caggcgcagg
     6001 atcgacatgt cggccacgct agccgatcgc catccctgcc gccagccacc tcgcggcgct
     6061 atgctgaaca ttcagtccca ccgtggagcc cggcatgcgt gctctccccc tgctggtcgt
     6121 gttggcgctg ctcggcgcct gcgccgggcc catgcccacc cccgatccgc agcaggcctg
     6181 gatcgagctg cgcagcccgc ccaccgagct gctgatggcc gagcggctgg acgacgtgcg
     6241 cctgaacgat ggccgctact tccaggtcag ccccggcgcc catcgcctgc tggcgaccta
     6301 tcgcttcgag gtgtcggtcg gcccgatcat gaccctcgag cccgtgcaga tgctctgcta
     6361 cctgcgcctc gactacgaca acttcgtcgc cggccagcgc taccgccttg aagcgcgcag
     6421 ctggctgttc cagaccgaat cctggctcag cgacgccagc ggcaagcgcc tggccgacgg
     6481 ccaggagtgg cactgcatta cgcagtgagc gtatgggcga attcattcgc caaagaccgc
     6541 accaaggcga atgaattcgc ccctacaggt ttgccgccaa ctccgcgcgt aaccactcgg
     6601 ccagctcgac ggcgcggcgg tcggcgccgc gcgccggcac ccacaacgcc aggcgtgccg
     6661 gggtttcgac gaagccccaa ggcgcctcca gccggccggc ggccaggtcg tcggcgacca
     6721 agagttgcgg ggcgatggcc ac
//
LOCUS       DATCUN010000106         6959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_135280, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000106 DATCUN010000000
VERSION     DATCUN010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135280"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VIO83_04025"
     CDS             complement(<1..883)
                     /locus_tag="VIO83_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8330475.1"
                     /translation="MNAARPLRLGVAGLGRAFTLMLPTLQRDARVRLVAACDPRDPAR
                     GRFASDFDAPVYADIEGLASNPDVEAIYIASPHQFHAEQARIAARHGKHVLVEKPMAL
                     SLGDCDAMIRDCREAGVYLIVGHCHSFDTPYLRAREIALGGELGRVRMIQALNYTDFL
                     YRPRRPEELRTEEGGGVVFSQAAHQVDIVRLLAGTRVSRVRAITGDWDPRRPTQGAYS
                     ALLWFEGGAFASVSYNGYGHFDSDEWCGWIGEMGAEKSPDAYGAARRKLAAVGSPEEE
                     ARLKAAGTYGGPGYVPAP"
     gene            complement(880..1818)
                     /locus_tag="VIO83_04030"
     CDS             complement(880..1818)
                     /locus_tag="VIO83_04030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEY8330476.1"
                     /translation="MPLRVARILDVARDIRSFELVQPDASALPPFTPGSHVKVQAPNG
                     MLRKYSLCNDPAERHRYVIAVKRDPAGQGGSLSMHEELHEGDTLPTSLPSNAFPLVEN
                     AKGYLFIAGGIGITPILSMIRSFGELPPAPWKLIYLTRFPETAAFREELGAPELKSRV
                     RIHHSHGDPARAFDLWPVLEKPNTAHVYCCGPRALMEAVRDMTGHWSPANVHFESFNE
                     GGGVRPDDKPFMVKLASTGAVFEVPVGKSILSVLHEHGCNAASSCESGTCGTCRTKLL
                     GGEADHRDMVLLPEEMDSQIMICVSRAKSDELVIDL"
     gene            complement(1869..2846)
                     /locus_tag="VIO83_04035"
     CDS             complement(1869..2846)
                     /locus_tag="VIO83_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY8330477.1"
                     /translation="MKHKIPAWLVGLACIAQLAFGSLAHAQAYPSRAIKLVVPYAAGG
                     LPDTVARILAKSLGDTLGQAVYVDNRPGAGGSVAASTILQSPADGYTLLVTDGPMLSI
                     TPLINKKITYDAANDFVPISLVGKAPLFLAVNANVKANTLEELVALAKAKPGGLNYGS
                     AGNGSIHHLTMEAMNASLGISMTHIPFRGSAVSVPAMIGGQVDMVFASPPSLMGFVKS
                     GQARLLATNSTTRSPLAPQMPALAEKIPGFDFAFTVAVLAKTGTPSAVISRINAEVNK
                     IVKVPEVIEQLRTAGVDPVGGSPEQLARALSAEKAQVTDAAARAQLKAE"
     gene            complement(2879..3868)
                     /locus_tag="VIO83_04040"
     CDS             complement(2879..3868)
                     /locus_tag="VIO83_04040"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8330478.1"
                     /translation="MSKLQLSVAMGDYDRTRALFDGRVQIDGVDPVYMLLNPEEMFFR
                     AMRSVDFDITELSFSSYLVKHSRGECPYIALPVFLSRAFRHTSIYVRKDRIREPADLK
                     GKRIGLPEYQLTANVWARAILQDDYGVRPSDVTWVRGGIETPGRPEKVKLQLPEDVRI
                     EQAPEGATISQMLDRGEIDGFMAPRPPSRSALANPNIGWLFDDPTAVAKDYYRRTGVF
                     PIMHVAAVRKELAAKHPWLPAALVKAFTQSKAAALELLSDTSATKVTLPFVEEQLQAA
                     REAMGDDFWSYGVAASARTLETFVRTHYEQGLSSRHVSVSELFHPATYETYSI"
     gene            complement(3873..5192)
                     /locus_tag="VIO83_04045"
     CDS             complement(3873..5192)
                     /locus_tag="VIO83_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017132943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8330479.1"
                     /translation="MMTHEENELLCRVEGDAPMGRLMRRHWMPICLVEEVSEPDGTPV
                     KARAFGEDLVVFRDSEGRIGVMDEYCPHRRASLVYGRNEEGGLRCLYHGWKMDVDGNV
                     LEMASEPASSGMVDKVKHTAYPTKEWAGMVWAYMGPKETMPEFQPPPWAPTADTRVSI
                     AKVLLPCNWAQILEGAIDSAHSSSLHSSDMVPARVDGAKATDKTWLRPSTDKAPRMQV
                     QRTGYGFRYAALRRPITNAAENDYVRSTVFVAPATALIPPNNLYNVANVNVPMDDTNT
                     AFYFIAWGRPSQTPETETWRKFLRQTVGVDLDQNYRPLRNEANKFWQDRNAMKAGNFT
                     GITGFPNQDVAMWLTMGAIADRTHDRLGASDLAIVEFRKQMLEAVKAFDEGAPAIGTG
                     AEAATPAVCSFQAIVPKTTDWRTYEARYVWLDSEDRPEFEPSYSVKS"
     gene            complement(5237..5698)
                     /locus_tag="VIO83_04050"
     CDS             complement(5237..5698)
                     /locus_tag="VIO83_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8330480.1"
                     /translation="MKPSENPAPIWSDSLLLGHAAIDGEHEQLAELIERMRRAAAPEM
                     AQALDAVLQHAASHFAAENELMRATAFPPRDCHISEHEAVLSTLRGVRARLERGEFKV
                     ARRVADELAAWFPPHVQHLDSALTHWLCKLQHGAKPVVLRVGRKSEAALAC"
     gene            6064..6921
                     /gene="nadC"
                     /locus_tag="VIO83_04055"
     CDS             6064..6921
                     /gene="nadC"
                     /locus_tag="VIO83_04055"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY8330481.1"
                     /translation="MTEREFLKQVHRDVERALDEDVGPGDLTAELVPAAARATATITC
                     RQAAVVCGRPWVEEILRRLAPTARADWRVPDGGTCVPGQAVVVIEGAARELLTAERTC
                     LNFLQTLSGVATKTARYVKEVEGTRAVVLDTRKTIPGLRAAQKYAVRCGGGQNHRMGL
                     YDAVLIKENHIAAAGGLTAAFRAAQALEGRVAFIQVEVETLRQLQEALDAGVGMVLLD
                     NMSLETVREAARLAQGRCSLEVSGGVTFETLRAYAETGVDRISVGALIKDVMAVDFSM
                     RFSEQPLEA"
BASE COUNT         1073 a         2294 c         2453 g         1139 t
ORIGIN      
        1 cgggcgcggg cacgtagccg ggcccgccat aggtgcccgc cgccttcagg cgcgcctcct
       61 cttccggcga gcccacggcc gccagcttgc ggcgcgcggc gccgtacgca tcgggcgact
      121 tctcggcgcc catctcgccg atccagccgc accattcgtc cgaatcaaag tgcccgtagc
      181 cgttgtagct gacgctggca aacgccccgc cctcgaacca cagcagggcc gaataggcgc
      241 cctgggtcgg gcgccgggga tcccagtcac ccgtgatcgc gcgcactcgg ctgacccgcg
      301 tgccggcgag caggcgcacg atgtcgacct ggtgggcggc ctggctgaac accacgccgc
      361 cgccctcctc cgtgcgcagt tcctcggggc gccgcggccg atagaggaag tcggtgtagt
      421 tgagcgcttg gatcatgcgc acccgcccga gctcgccgcc cagtgcgatc tcgcgtgcgc
      481 gcaggtacgg cgtatcgaag ctgtggcagt ggccgacgat caggtacacg ccggcttccc
      541 ggcaatcccg gatcatcgcg tcgcagtcgc ccagggacag cgccatgggc ttttcgacga
      601 gcacgtgctt cccgtgccgg gcggcgatgc gcgcctgctc cgcatggaac tggtgcgggc
      661 tggcgatgta gatcgcctcg acgtccgggt tggacgcaag gccctcgatg tccgcgtata
      721 ccggcgcatc gaagtcgctc gcgaagcggc cgcgcgcggg atcccgcgga tcgcaggccg
      781 ccaccagccg cacgcgcgcg tcgcgctgga gcgtgggcag catgagcgtg aaggcgcggc
      841 cgaggccggc cacgccgagt cgaagcgggc gggcggcgtt cacaggtcga tcaccagttc
      901 gtcggacttg gcccgggaga cgcagatcat gatctggctg tccatctcct cgggcagcag
      961 caccatgtcc cggtggtccg cctcgccgcc cagcagcttc gtgcggcagg tgccgcaggt
     1021 gccgctctcg cacgaggacg ccgcgttgca gccgtgctcg tggaggaccg acaggatgga
     1081 cttgccgacg ggcacctcga acaccgcgcc cgtgctggcc agcttcacca tgaagggctt
     1141 gtcgtcgggc cgcacgcccc cgccctcgtt gaagctctcg aaatgcacgt tggccggcga
     1201 ccagtggccg gtcatgtcgc gcaccgcctc catcagcgcg cgcgggccgc agcagtacac
     1261 gtgggccgtg ttgggcttct ccagcaccgg ccagaggtcg aatgcgcgcg cggggtctcc
     1321 gtggctgtgg tggatccgca cgcggctctt gagctcgggg gcgccgagct cctcgcggaa
     1381 ggctgcagtc tcgggaaagc gcgtcaggta gatgagcttc cacggcgccg gcggcagctc
     1441 gccgaacgac cggatcatgg acaggatcgg cgtgatgccg atgccgcctg cgatgaagag
     1501 atagcccttg gcgttctcga ccagcgggaa ggcgttcgac ggcagcgacg tgggcagcgt
     1561 gtccccctca tgcagctcct catgcatgga gagcgagccg ccctgccccg cgggatcacg
     1621 cttgacggcg atgacgtagc ggtggcgctc cgcgggatcg ttgcacagcg aatacttgcg
     1681 cagcatgcca ttgggggcct ggaccttgac atgcgatccc ggcgtgaacg gcggcagcgc
     1741 gctggcatcg ggttgtacca gctcgaagct gcggatgtcg cgcgcaacgt cgaggatccg
     1801 cgccacgcgc agcggcatct gcgtgtcgga atgggttggg ggtggggtga ccgtcatgac
     1861 gcgcggaact actcggcctt cagctgggca cgcgcggcgg cgtcggtcac ctgggccttc
     1921 tcggcgctca gcgcccgcgc gagctgttcg ggcgatccgc ccaccggatc gacgcccgcg
     1981 gtgcgcagct gctcgatgac ctcggggacc ttcacgatct tgttgacctc agcattgatc
     2041 ctggaaatga ccgcgctcgg cgtaccggtc ttggccagca ccgcgaccgt gaatgcgaag
     2101 tcgaagccgg ggatcttctc tgccagcgcg ggcatctgcg gagccagcgg agaacgcgtc
     2161 gtcgagttgg tggccagaag gcgcgcctgg cccgacttga cgaagcccat gagcgacggc
     2221 ggcgaggcaa acaccatgtc cacctgcccg ccgatcatgg cggggaccga gacggcgctg
     2281 cccctgaacg gaatgtgcgt catcgagatg cccaggctcg cattcatcgc ctccatcgtc
     2341 aggtggtgga tgctgccgtt gccggcggag ccgtagttga ggccccccgg cttcgccttg
     2401 gccagcgcga cgagctcctc cagcgtgttc gccttgacgt tcgcgttgac ggcgaggaac
     2461 agcggggcct tgcccaccag ggagatcggc acgaagtcgt tggccgcgtc gtaggtgatc
     2521 ttcttgttga tcaacggcgt gatcgacagc atcggcccgt cggtcaccag cagcgtgtag
     2581 ccgtcagccg gggactgcag gatcgtggag gcggcgaccg agccacccgc gccgggccgg
     2641 ttgtcgacat agaccgcctg tcccagcgtg tcgcccagcg acttggcgag gatgcgcgcc
     2701 actgtgtcgg gaaggccgcc agcggcatag ggaacgacca gcttgatggc gcggctcgga
     2761 taggcctggg catgggccag ggagccgaac gcgagttggg cgatacaggc caagccgacc
     2821 agccaggcgg ggatcttgtg cttcatgggg gatgtctcca gttgtgagtg atgcggaatc
     2881 agatgctgta ggtctcgtag gtcgcggggt ggaagagctc gctgaccgac acgtggcgcg
     2941 acgacagtcc ctgctcatag tgggtccgca cgaaggtctc cagcgtcctg gccgaggccg
     3001 ctacgccgta cgaccagaag tcgtccccca tcgcctcgcg ggcggcctgc agctgctcct
     3061 cgacgaaggg cagcgtgacc ttggtggccg aggtgtccga cagcagctcc agcgcggcgg
     3121 ccttggactg ggtgaaggcc ttgacgagcg cggcgggaag ccaggggtgc ttggcagcga
     3181 gctccttcct gacggccgca acgtgcatga tcgggaacac gcccgtacgc cggtagtagt
     3241 ccttggccac cgcggtcggg tcgtcgaaca gccagccgat gttcggattg gcgagcgccg
     3301 accggctcgg cgggcgcggc gccatgaagc cgtcgatctc gccgcgatcg agcatctggg
     3361 agatcgtggc cccctcgggc gcctgctcga tccggacgtc ttccggcagc tgcagcttga
     3421 ccttctcggg ccgtcccggc gtctcgatgc ccccgcgcac ccaggtcacg tcggaggggc
     3481 gcacgccgta gtcgtcctgc aggatcgcgc gcgcccagac gttggcggtg agctggtact
     3541 cgggcaggcc gatgcgcttg cccttcaggt cggccggctc gcggatgcgg tccttgcgca
     3601 catagatgga cgtgtgccgg aacgcccgcg agagaaagac cggcagcgcg atgtaggggc
     3661 actcgccgcg cgagtgcttc accaagtagc tggagaacga gagttccgtg atgtcgaaat
     3721 cgacgctgcg catcgcccgg aagaacatct cctcgggatt gaggagcatg tacacgggat
     3781 cgacgccgtc gatctggacg cggccgtcaa acagcgcacg ggtgcggtcg tagtcgccca
     3841 tggcgacgga gagttggagt ttgctcacgg gatcaggact tcacggaata ggagggttcg
     3901 aactcggggc ggtcttcgct gtccagccag acgtagcggg cctcgtaggt gcgccagtcg
     3961 gtcgtcttgg ggacgatggc ctggaaggaa cagacggccg gcgtggccgc ttcggcgccg
     4021 gtgccgatcg ctggcgcgcc ctcgtcgaac gccttgaccg cctcgagcat ctgcttgcgg
     4081 aactccacga tcgccaggtc gctggcgccg agccggtcgt gcgtgcggtc ggcgatggcc
     4141 cccatggtca gccacatcgc gacatcctgg ttcgggaagc ctgtgatgcc tgtgaagttg
     4201 ccggccttca tggcgttgcg gtcctgccag aacttgttcg cctcgttgcg caggggacgg
     4261 tagttctggt ccaggtcgac acccaccgtc tgacgcagga acttgcgcca ggtttcggtc
     4321 tccggggtct gcgacggacg gccccaggcg atgaaataga acgccgtgtt cgtgtcgtcc
     4381 atcggcacgt tgacgttggc cacgttgtag aggttgttgg gcgggatcag ggccgtggcc
     4441 ggggccacga agaccgtcga gcgcacgtag tcgttctccg cggcgttcgt gatcggacgg
     4501 cgcagcgcgg catagcggaa gccatagccg gtgcgctgca cctgcatgcg cggggccttg
     4561 tcggtcgagg ggcgcagcca ggtcttgtcg gtggccttcg cgccatcgac ccgggccgga
     4621 accatgtcgg aggaatggag gctggagctg tgggccgagt cgatggcgcc ctcgagaatc
     4681 tgcgcccagt tgcacggcag cagcaccttc gcgatgctca cccgggtgtc cgccgtcggc
     4741 gcccacggcg gcggctggaa ctcaggcatg gtctccttcg gccccatgta ggcccacacc
     4801 ataccggccc attccttggt cgggtacgcg gtgtgcttga ccttgtcgac catgccgctc
     4861 gaagccggct cggatgccat ctccagcacg ttcccgtcga cgtccatctt ccagccgtgg
     4921 tagaggcagc gcaggccgcc ctcctcgtta cggccgtaga ccagcgacgc acgacggtgg
     4981 gggcagtact catccatgac cccgatccgg ccttcgctgt cccggaagac cacgaggtcc
     5041 tcgccgaacg cccgcgcctt caccggcgtg ccgtcgggct cgctcacctc ttccacgagg
     5101 cagatgggca tccagtggcg gcgcatcagg cgtcccatgg gggcgtcgcc ttcgacgcga
     5161 cagagcagtt cgttttcttc gtgagtcatc atggtgtcat ctcatctatc aggggtggtt
     5221 gaaccagggg tttgaactag caggccaggg cggcctcgga tttgcggccc acgcgcagca
     5281 ccacgggctt ggccccgtgc tgtagcttgc agagccaatg ggtaagtgcc gaatcgaggt
     5341 gctggacgtg gggcggaaac catgccgcca gctcgtccgc cacccggcgg gccaccttga
     5401 actccccacg ctccaggcgt gcgcgcacgc cgcgcagcgt ggaaagcacc gcttcgtgct
     5461 cgctgatgtg gcagtcgcgc ggcggaaacg ccgtcgcgcg catgagctcg ttctcggccg
     5521 cgaaatgtga ggccgcatgc tggagcaccg catccagcgc ctgcgccatc tccggcgcgg
     5581 cggcgcgccg catgcgctcg atcagctcgg ccagctgctc gtgctcgcca tcgatcgcgg
     5641 catgcccgag aagcagcgag tctgaccaga ttggcgccgg gttttcagag ggcttcatgg
     5701 tggggagtac tggcaccgct ggcgatgcct cgttttgtta gttgcctaat aattctaggt
     5761 gcacgcgttg atccaggcaa cggttttccc tccggggttt ccctagaatc atttggcctc
     5821 taataatttt gtaaactcgg gatcaatgtg tacgcatggc cgtggattcg tgtgcgtgcg
     5881 caccggctcg gcccgccgtc gaacgccttc tgccttctga agacgcgcgc gtgccgcgag
     5941 gccgggaagc gacggtgccg ccaggggccc ctgcggctcc gtcccgcacg gacgcacgcc
     6001 atggaccggc gcgatggcgc cccgccggga cgcggactcc ccaggacgga taaaggatcg
     6061 aaagtgaccg agagagagtt tctgaagcag gtgcaccgag acgtcgaacg cgcactggac
     6121 gaggatgtcg gcccgggaga cctgaccgcg gaactggtcc cggcggccgc ccgcgccacg
     6181 gccaccatca cgtgccgcca ggcggcagtg gtctgcggcc ggccctgggt cgaagagata
     6241 ctgcgtcgcc tggcgccaac ggcgcgggcc gactggcggg tacccgatgg cggcacctgc
     6301 gtgcccggcc aggccgtcgt ggtgatcgaa ggcgcggcgc gcgaactcct caccgcggag
     6361 cgcacctgcc tgaactttct gcagacgctc agcggcgtgg cgaccaagac cgcccgctat
     6421 gtcaaggagg tcgaaggcac gcgcgccgtc gtcctggaca cgcgcaagac catccccggc
     6481 ctgcgcgccg cgcagaagta cgccgtgcgc tgcggcggcg gccagaacca ccggatggga
     6541 ctgtacgacg ccgtcctgat caaggagaac cacatcgcgg cagcgggcgg cctgacggcc
     6601 gcgttccgcg cggcgcaggc actcgagggg cgggtggcct tcatccaggt cgaggtagag
     6661 acgctgcggc agttgcagga agcgctcgac gcgggcgtcg gcatggtgct gctggacaac
     6721 atgtccctgg agacggtgcg cgaagcggcg cggctggccc agggccgctg cagcctggag
     6781 gtctccggcg gcgtcacctt cgagacgctg cgggcctacg ccgagaccgg cgtcgaccgc
     6841 atatccgtgg gcgcgctgat caaggacgtg atggccgtcg acttctcgat gcgcttcagc
     6901 gagcagccgc tggaggcctg acccgggagc gcgtcgcgcc ggcgccggcg cgcatgccg
//
LOCUS       DATCUN010000107        25336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_42508, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000107 DATCUN010000000
VERSION     DATCUN010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 25336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25336
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42508"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /gene="acnA"
                     /locus_tag="VIO83_04060"
     CDS             complement(<1..1514)
                     /gene="acnA"
                     /locus_tag="VIO83_04060"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY8330482.1"
                     /translation="MNTQYRKSLAGTQLDYFDVREAVDAIAPGAYAKLPYTSRVLAEQ
                     LVRRCDPAMLTDSLKQLIERKRDLDFPWYPARVVCHDILGQTALVDLAGLRDAIAEQG
                     GDPAKVNPVVPTQLIVDHSLAVEAPGFDPDAFAKNRAIEDRRNEDRFHFIEWTKTAFK
                     NVDVIPAGNGIMHQINLEKMSPVIQARGGVAFPDTCVGTDSHTPHVDALGVIAIGVGG
                     LEAETVMLGRASMMRLPDIVGVKLSGKHQPGITCTDIVLALTEFLRKERVVGAYVEFF
                     GEGAASLSIGDRATISNMCPEYGATAAMFYIDQQTIDYLKLTGREPEQVALVENYAKT
                     TGLWADALVEAEYERVLEFDLSTVVRNMAGPSNPHRRLPTSALHERGIADEGKLAAAR
                     AEEAEGLLPDGAVIIAAITSCTNTSNPRNVVAAGLLAKKANELGLLRKPWVKTSFAPG
                     SKVAKLYLEEAGLLPELEKLGFGIVGYACTTCNGMSGALDPQIQQEIIDRDLYAT"
     gene            complement(1516..2679)
                     /gene="prpC"
                     /locus_tag="VIO83_04065"
     CDS             complement(1516..2679)
                     /gene="prpC"
                     /locus_tag="VIO83_04065"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEY8330483.1"
                     /translation="MTATVETTTVGFKPKKSVALSGTTAGNTALCTVGRSGNDLHYRG
                     YDILDVASTCEFEEIAHLLVHGKLPNVAELASYKTKLKSLRGLPAGVKAALEQLPPSA
                     HPMDVMRTGTSVLGCLSPEKDDHNHPGARDIADKLMASLGSMLLYWYHFSHNGKRIEV
                     ETDDDSIGGHFLHLLHGVKPRDSWVRAMHTSLNLYAEHEFNASTFTSRVIAGTGSDMF
                     SCITGAIGALRGPKHGGANEVAFEIQKRYDNPDEAEADIRARVERKEVVIGFGHPVYT
                     VADPRNKVIKEVARELSAEQGSTKMFDIAERLESVMWEIKKMFPNLDWFSAVSYHMMG
                     VPTAMFTPLFVIARTSGWSSHVIEQRVDGKIIRPSANYVGPEDLKFVPLKDRK"
     gene            complement(2759..3643)
                     /gene="prpB"
                     /locus_tag="VIO83_04070"
     CDS             complement(2759..3643)
                     /gene="prpB"
                     /locus_tag="VIO83_04070"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEY8330484.1"
                     /translation="MSNKTPGQLFREAIANEHPLQVIGAINANHALLAKRAGFKAIYL
                     SGGGVAAGSLGLPDLGINTLDDVLTDVRRITDVCDLPLLVDIDTGFGPSAFNIERTVK
                     SLIKAGAAAAHIEDQVGAKRCGHRPGKEIVSTEEMADRVKAAADAKTDPNFFLIARTD
                     AIQAEGVDAAIERCLRYVEAGADAIFAEAAYDLPTYKRFVDALNVPVLANITEFGATP
                     LFSRDELASVGVAIQLYPLSAFRAANKAAEAVYTAIRRDGHQKEVIDLMQTRVELYDR
                     IGYHAFEQKLDALFAAKK"
     gene            complement(3640..4362)
                     /locus_tag="VIO83_04075"
     CDS             complement(3640..4362)
                     /locus_tag="VIO83_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010429381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8330485.1"
                     /translation="MMEAAAETQIVDEEVGTLSDHVFQRIQSAIVRGDIAPGSKISEP
                     ELARAYGISRGPLREAIRRLEGQRLLVRVPHVGARVVSLTQAELVELYEIRESLEGMA
                     CRLAAERMSQAEIDDLRHVLETHQSDEAFRAGRGYYQQEGDFDFHYRIIQGSGNRTLS
                     NLLCNELYQLVRMYRIQFSATPNRPQQAFTEHHRILDAIAARDGELAELLMRRHIAAS
                     KKNIARHYQGATHKHVTQRGKS"
     gene            4658..5194
                     /locus_tag="VIO83_04080"
     CDS             4658..5194
                     /locus_tag="VIO83_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease"
                     /protein_id="HEY8330486.1"
                     /translation="MRLKPLVLLLSLLLPCGLALAAEKTVYGLHEYARLQELGTVLAA
                     KLDTGAKTASLSAHNIKRFKRNGEPWVRFTPGSDENDNTVIEKPLARISKIKRRAGDF
                     DPDEGKTYTARPVIELDVCMGTALRTIEVNLTDRSAFEYPLLIGSDALKRFGALVDPS
                     LKYAAGQPGCPNVAPSDE"
     gene            5200..6726
                     /locus_tag="VIO83_04085"
     CDS             5200..6726
                     /locus_tag="VIO83_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inactive transglutaminase family protein"
                     /protein_id="HEY8330487.1"
                     /translation="MRSLTLHLKVLIAILVSLGVAITAYQIFVLGIPVTEAETDNVWS
                     IDAKVEFQANPRNPIKVQMFVPPLGQDFVSLNESFISNNYGVSVNNVDGNRRVTWSAR
                     RANGPQTLYYRMALTQRFTAEKPKEEGPIYREGLPLAGPEQVAAEALLAPIRQHSADV
                     ETFISETIKRVNNLNDDNVKLLLAGDTSVEKRARVIDLLLSIGHVPLEQVHTIRLIEN
                     QPQTPELWLRSFNGENWLYFNPDNGQQGLPRDRLIWWLGDAPLLSVDGGKQAQVNFTL
                     NTSELNAISLAKQSDASVDSGFLEYSLYGLPLQTQQTYQIMIMIPIGVLVILILRNLG
                     GLQTLGTFTPVLIALAFRETQLGFGIMLFTIITALGLSLRSYLEHLKLQMLPRLSVVL
                     TFVVVLIAVISLFSHKLGLERGLSVALFPMVILTMTIERLSITWEERGGGHAFKVAVG
                     TLIAATLAYLLMTIPELTYFIFTFPAVLLILVGFMLAMGRYRGYRLTELFRFKAFLKD
                     "
     gene            6731..7717
                     /locus_tag="VIO83_04090"
     CDS             6731..7717
                     /locus_tag="VIO83_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HEY8330488.1"
                     /translation="MFGLIKRWKALSAKGIMGINRRNADYVLKYNKRSLYPVVDDKIL
                     TKERAIKAGIHVPEMYGIISTEKEIENLDKIIGEHNDFVIKPAQGAGGDGILVIADRF
                     EGRFKTVSGKIVSRDEIEQQISSILSGLYSLGGGRDRALIEYRVTPDPIFKSISYEGV
                     PDIRIIVLMGYPVMAMLRLPTRQSGGKANLHQGAIGVGVDLATGLTLRGTWLNNKITK
                     HPDTTNAVDGVQLPNWDGFMKLAAGCYELCGLGYIGVDMVLDVEKGPLILELNARPGL
                     NIQIANDCGLTHRAHAVETRLEELKAKGIQETPEQRVRFSQELFGHVQPAEF"
     gene            7849..9198
                     /gene="pabB"
                     /locus_tag="VIO83_04095"
     CDS             7849..9198
                     /gene="pabB"
                     /locus_tag="VIO83_04095"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEY8330489.1"
                     /translation="MSICRVYPLPYHADPRSRFALVRAAPGACLLDGGQPAAERGRYD
                     LFSAWPERHLQAQAGEDGRQFLARCRVALAELGRAEAPDDCELPFTGGLLGYLGYDFG
                     RRLERLPQHAAADLALPDAAFGVYAWALVSDHQRRTSQLVFHPSLAEPRRQALIALFT
                     GVQPAAAPGPFRLSQPFQADLSAAQYRQAIEKIQAYIRAGDCYQVNFAQRFQAPWQGD
                     PWRAYLALRDACPTPFAGFLRLDRGTLLSLSPERFIRVSRGQVETRPIKGTRPRGRNS
                     EEDRALAEELLDSRKDRAENLMIVDLLRNDLGRSCRIGSVRVPELFALESYPNVHHLV
                     SAVTGELAPGLDAFDLLAGSFPGGSITGAPKIRAMQIIDELEPTRRSIYCGSLLYIDS
                     RGEMDSSICIRTLLLQDGQASCWGGGGIVMDSEWQAEYEESKTKVRVLLQTLEATLD"
     gene            9301..12975
                     /locus_tag="VIO83_04100"
     CDS             9301..12975
                     /locus_tag="VIO83_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8330490.1"
                     /translation="MPSLNRIILINTHLQGVVELVVDDHTNICGTNASGKTTLQRLVP
                     VFYGEYPSRVVPATRDSFERWYLPTEQSFIVYEYRRMDDQLCQAVLAAASDGKGVAYR
                     LVHKAFALDDYIKSRQGDAITCLNMAELGRQLKQAGVPVSTLLNTRQFRAIIQNDRSL
                     LAADGQRAELRSWARQFSLCDAEHSLRHIEKLARAVHSREGKMETVKSMVAAILEEDG
                     VSPPATHLNLQRVEDWIRDSRLIQGFTAIRPEFDKLEREYQDLLACEARLGGLLLGYR
                     ADEPRLYQRQEETTAVIEQTGFRLGQLEERWKDQRDTLNQDLSAAKGDIGRIGDELDQ
                     IDNQYQTFLDADIEQAKADLEQLGAWRDDLGNLQARHRLLTDQHQDVERAYLERQQAI
                     KDQREAALELLRGQEDALREERDGQKAQQDEALGALEKAFAARRQQGQEDFTQREYAL
                     KLERGRLEQQIASVGYSEEENLALAILDRRLEEADEKGEAAEARVRQLEGQERQLRSR
                     RDEANTAFALAARRAAERQEQLEDLERALYPGQHTLLEFLRREQPGWEARLGKVIHPA
                     LLQRSDLKPAVVDVDPDSLFGLQLDLAALDIPEHAASEQELRQRVQLAKDALQDARNQ
                     QQEAESRLVEASEALDELQRQLVVARSEAQNRRDDRQRLKEERRSQKDKLDAAVQERR
                     SQARKQLVELDNALKQLASARDGWLSELKAQHSEERLETSAHWQQVIGSLDSQIRQLR
                     AAADERRANAQAELAQCEHWYQAELKSRGVDETVIIELKRQIKGLTDRIARTEQRRAD
                     VLRYDDWYRHSWLQRKPKLQAELGDSRARAATLEQQLKTASAEYKAERSQLETTRQQA
                     LQQKGALDDALTRLKALLRRLGELKLSRDGEIAEGELDERLRLGEEVLHGREQMLAAI
                     KAHVEHFDSLIVGQSGSSLAETWERSREECTLANERGIPSLDYRRLVPHLAQLLNVMV
                     PQKLTGLREQGKIFGLDLHGYYEVLADIDRRIATQSARITREVSEELFLDGVSESAVR
                     IRSRISELEFWPELKAFIKAFREWREAGFSELPGEEYTTSMRRALDIIGRSALSGGIA
                     ALLEIELRLREGNSDLVIRTDRQLNESSSHGMAYLILCKFLLAFTRLLRGDAPATIHW
                     PIDELGTLHHNNVKKIFDACSSNNIRILGAFPNPDSEVLSLFANRYIVNKQTRQLQVV
                     KPQLDVIAEKLKGLKTGEVA"
     gene            12976..13617
                     /locus_tag="VIO83_04105"
     CDS             12976..13617
                     /locus_tag="VIO83_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330491.1"
                     /translation="MLSTKGQVIERLLKGDFICRTTDEDGWRFLKTPANREQVDDYLA
                     TLNRMVATVGSGADSDVFFCGYRQLDEDERKVVGQQFRDVCNALTPLVEWLVLVQEAG
                     AQDAPLTEGSALRLNELQSIVEDTPAFREQLARISHYRLFGSTSATVDGQIKQVFKRL
                     CELGYLVRPNPDKQIYLGTGKLDYLYEVIRFIDETESLDLEERAESASQGSLL"
     gene            13614..14834
                     /locus_tag="VIO83_04110"
     CDS             13614..14834
                     /locus_tag="VIO83_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY8330492.1"
                     /translation="MSGNLHQAGVRFLRQLGRHAEPIMDAYLAGSLPDQALEPAVQER
                     LVKDGILYRPEPGADLHLRRVVRALLEEALRDDRNRQIDANTGSALVTFKTLAAHYKE
                     ARHQGDYAAADAYLADLKEHVYAFGETLSHGIRVLWSRINNEFGYVGTLNAKIRENEL
                     AQSQVSELLAGLEMISFAELADTAGDLRELRRLLVTSLQRTVSACSQELSIVQGRLLE
                     LLGRFRQIRGRTRLLKGWLLHMEQQPDYQVGNHAAHQNVPQLFNQAAAVLAPAAVDIH
                     NPLHEAQLLALVAQIRALQPPDRFAPAPAEAGEFVLGEVEAFEIEHNPIREAVSAYFC
                     QVIDSGQPISALAWRAQQALPWDAESWLYQVIGGYEGLPEEQKRHFELDPIGEAHPLY
                     SGNFIVRDVRLWLA"
     gene            14822..15655
                     /locus_tag="VIO83_04115"
     CDS             14822..15655
                     /locus_tag="VIO83_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330493.1"
                     /translation="MAGLSRIHQQAVHDLLRGRLERVALNATWRRIQVHFEVGTFDGR
                     WLQFDRQQREILRQLAQQEWGFDPLEGVPDGTRLEVAGQAIDEKTARQRPDDSHVLVK
                     GALPAPLPTLPPELSLRVPLASLDLAAITQVLVIENLDSFDDWQHYVAPIELAGSLLL
                     YRGHGGLARGVHQLLNSLPASTRVIVFPDYDPAGLKIAATSPRATALLLPEPDKHLLA
                     KGSRQHFDEQFQEARYLDGAELGSWQPVWDEMKARQVSIKQQHMLALGVRLRIVPRHP
                     R"
     gene            complement(15725..16342)
                     /gene="thrH"
                     /locus_tag="VIO83_04120"
     CDS             complement(15725..16342)
                     /gene="thrH"
                     /locus_tag="VIO83_04120"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HEY8330494.1"
                     /translation="MEIACLDLEGVLVPEIWIAFAEKTGIDALKATTRDIPDYDVLMK
                     QRLRILDEHGLKLRDIQEVIATLKPLDGAVEFVDWLRERFQVVILSDTFYEFSQPLMR
                     QLGFPTLLCHKLITDETDRVVDYQLRQKDPKRQSVIALKSLYYRVIAAGDSYNDTTML
                     GEAHSGILFHAPENVIREFPQFPAVHTYEDLKQEFLKASVRDLSL"
     gene            16579..17313
                     /locus_tag="VIO83_04125"
     CDS             16579..17313
                     /locus_tag="VIO83_04125"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY8330495.1"
                     /translation="MSQTFDVAALAAAYADKSPQEVLKLAFEHFGDELWISFSGAEDV
                     VLVDMAWKLNKNVKVFSLDTGRLHAQTYRFIEQVREHYGIQIEVLSPDPRLLEPLVRE
                     KGLFSFYKDGHGECCGIRKIEPLKRKLATVKAWATGQRRDQSPGTRSQVAVLEVDGAF
                     STPEQPLYKFNPLANMSSEEVWGYIRMLEIPYNPLHEQGFISIGCEPCTRPVLPNQHE
                     REGRWWWEEATHKECGLHAGNLIAKN"
     gene            complement(17382..18356)
                     /gene="cysB"
                     /locus_tag="VIO83_04130"
     CDS             complement(17382..18356)
                     /gene="cysB"
                     /locus_tag="VIO83_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HEY8330496.1"
                     /translation="MKLQQLRYIWEVAHHDLNVSATAQSLYTSQPGISKQIRLLEDEL
                     GVEVFARSGKHLTRVTPAGERIITTAGEILRKVESIKQIAQEFSNEKKGTLSIATTHT
                     QARYALPEVISAFIKQYPEVALHMHQGSPSQIAEMAADGTVDFAIATEALEQFGDLVM
                     MPCYRWNRCVIVPQGHPLTRLPKLTLEALAEQPIVTYVFGFTGRSKLDEAFSHRGLEP
                     KVVFTAADADVIKTYVRLGLGVGIVAGMAVDPELDKDLVVLDASELFESSVTKIGFRR
                     GTFLRGFMLDFIERFAPHLTRERVAEAVQCRNKAELEELFADVVLPSH"
     gene            complement(18472..18969)
                     /locus_tag="VIO83_04135"
     CDS             complement(18472..18969)
                     /locus_tag="VIO83_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003152906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8330497.1"
                     /translation="MIRSILYATDLGLFAPYVLQHALLLARTHRADLYVLHVVEPLGL
                     FAESVLQTYLDSDSYNDLRLRGLDSVMASIEQRVFEGFCDELRESDGESVPIRAVRVQ
                     LGDPPQVILQEAQDLAVDLIVIGSHSQSMPACSALGRTCLRLLQQGRVPVYLVPILPE
                     EPALQ"
     gene            complement(19099..20055)
                     /locus_tag="VIO83_04140"
     CDS             complement(19099..20055)
                     /locus_tag="VIO83_04140"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-dehydropantoate 2-reductase"
                     /protein_id="HEY8330498.1"
                     /translation="MSASARPRIGIIGTGAIGGYYGLMLARAGFEVHFLLRSEYAAVA
                     RDGLRVRHAQLGELHLPNVNVNAWREAAAMPACDWLLVGAKTTSNRELAPLIAQVAAP
                     GAKVVLLQNGLGVEDALRPLLPESLHLLGGLCFICAHREAPGVVVHQAQGVCNLGYHS
                     GPATAEERQALLEQGVSWLREAGIESSVIADLNQARWQKLVWNVPYNGLSVLLHSSTR
                     PLMANPDSRALLQALMQEVVDATLACGYSLPDNYAQKLLAYTDRMPDYLPSMYHDFAQ
                     RREAELPALYAAPLAAAAAAGVDMPRVRMLYQALAFLQAAQQ"
     gene            20327..21403
                     /locus_tag="VIO83_04145"
     CDS             20327..21403
                     /locus_tag="VIO83_04145"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY8330499.1"
                     /translation="MADLPIDDLNIASNEALITPEQLKQELPLTETARHTVSHGRQVV
                     RDILDGKDHRLFVVVGPCSIHDIKAAHEYAERLKSLAAEVSDTLYLIMRVYFEKPRTT
                     VGWKGLINDPYLDDSFKIQDGLHIGRKLLCDLAEMGLPTATEALDPISPQYLQDLISW
                     SAIGARTTESQTHREMASGLSSAVGFKNGTDGGLTVAINALQSVSSAHRFLGLNQQGQ
                     VSIVTTKGNRYGHVVLRGGSGKPNYDSVSVALCEQELTKAGIRPNIMVDCSHANSNKD
                     PALQPLVMENVANQILEGNNSIIGLMVESNLGWGSQSIPKNLCELKYGVSVTDACIDW
                     DSTEKTLRSMHAKLKDVLPKRHRG"
     gene            complement(21464..21748)
                     /locus_tag="VIO83_04150"
     CDS             complement(21464..21748)
                     /locus_tag="VIO83_04150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8330500.1"
                     /translation="MSEPLSIQHDLAGKQFEVTVDGYRAYLSYMDLGKQTIDIYRTFV
                     PDALRGRGVAAALTEQALQFAEGRGYTVIPSCSYVERYLERRQRNGERQA"
     gene            22021..22272
                     /locus_tag="VIO83_04155"
     CDS             22021..22272
                     /locus_tag="VIO83_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpp/OprI family alanine-zipper lipoprotein"
                     /protein_id="HEY8330501.1"
                     /translation="MNNVLKISALTLAAILATGCSSMSKETEARLTATEDAAARAQAR
                     ADEAYRKADEALAAAQKAQQTADEANERALRMLEKASRK"
     gene            complement(22434..23360)
                     /locus_tag="VIO83_04160"
     CDS             complement(22434..23360)
                     /locus_tag="VIO83_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019092883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY8330502.1"
                     /translation="MLASLAALLLAAPASALELPLPPPGEDIVGEVQVIKAKYEDTFA
                     DIGTANDLGYLEMVAANPGVDPWLPGVDTEIILPTRFILPPGPREGIVINLAEYRMYY
                     FPKGQNVVHTYPLGIGREGWGSPITRTSIAAKTPNPTWTPPASIRKEHAAEGDPLPAV
                     VPAGPDNPLGPFKFTLGTPGYLIHGSNKRFGIGMRVSHGCFRMLNHNVLALAKIVPVG
                     TSVRIINEPYKFGIRGGKIYLEAHTPLDDHGEPSIVDKHTQVINALLQREDLKDDARL
                     DWEVVREVVAAEDGLPVEIAAPREQVASVVAH"
     gene            complement(23456..23737)
                     /locus_tag="VIO83_04165"
     CDS             complement(23456..23737)
                     /locus_tag="VIO83_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330503.1"
                     /translation="MSRQSWSLYAYRLLVPDEQLDLFACQEVRMHLVARQLELGDPGD
                     HTLCGSLLPAHPLWEVLDRASLKDRRLCSTCLQRLRAERHGLPRDWPVR"
     gene            complement(23798..24403)
                     /locus_tag="VIO83_04170"
     CDS             complement(23798..24403)
                     /locus_tag="VIO83_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011062550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY8330504.1"
                     /translation="MRTWLLRGCLGLLLLAQNAAAGTLLVVGDSISAAFGLETRDGWV
                     NLLASRLQREAPDWRVVNASVSGDTSAGGLARLPALLETHAPQLVLIELGGNDGLRGQ
                     PPLQLQQNLAAMIEQSRAAGAQVLLLGMKLPPNYGPRYSSAFEQVYAELAEQEQVALV
                     PFLLEGVGGVRALIQADGIHPTAEAQSILLENVWPTLQPLL"
     gene            24414..25094
                     /locus_tag="VIO83_04175"
     CDS             24414..25094
                     /locus_tag="VIO83_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8330505.1"
                     /translation="MSASILNARNLSKVVDSAEGALTILDDLTLELKRGDSLAIVGRS
                     GSGKSTLLGLLAGLDQPSSGEVQLAGHALSRLDEDQRARVRAEHVGFVFQSFQLIDSL
                     TALENVMLPLELEGRADARQSARSLLERVGLGQRLSHYPRQLSGGEQQRVAIARAFVG
                     QPDILFADEPTGNLDSQTGERISDLLFQLNHEHGTTLVLVTHDARLAARCQGQLRLEG
                     GRRVETQG"
     gene            25094..>25336
                     /locus_tag="VIO83_04180"
     CDS             25094..>25336
                     /locus_tag="VIO83_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330506.1"
                     /translation="MRAPSLARLLRLSLRQLLREARAGELRVLFFALLIAVAASSAIG
                     YFSARLNGAMLLRAGEFLAADLVLRGTAPAAPEQIAT"
BASE COUNT         4586 a         8640 c         8076 g         4034 t
ORIGIN      
        1 gtggcgtaca ggtcgcggtc gatgatttcc tgctggatct gcggatccag ggcgccggac
       61 atgccgttgc aggtggtgca ggcgtaaccg acgatgccga agccgagctt ctccagttcc
      121 ggcagcaggc cggcttcttc caggtacagc ttggcgacct tggagcccgg ggcgaaggag
      181 gtcttcaccc acggcttgcg cagcaggccc agctcgttgg ccttcttggc cagcaggccg
      241 gcggcgacca cgttgcgcgg gttggaggtg ttggtgcagc tggtgatggc ggcgatgatc
      301 accgcgccgt ccggcagcag gccttcggct tcttcggcgc gggccgcagc cagcttgcct
      361 tcgtcggcaa tgccacgctc gtgcagggcc gaggtcggca ggcgacggtg cgggttggac
      421 gggccggcca tgttgcgcac gacggtggac agatcgaatt ccagcacgcg ctcgtattcg
      481 gcctcgacca gggcatcggc ccacaggccg gtggtcttgg cgtagttctc gaccagcgcc
      541 acctgctccg gctcgcgacc ggtcagcttg aggtagtcga tggtctgctg gtcgatgtag
      601 aacatcgccg cggtagcgcc gtattccggg cacatgttgg agatggtcgc gcggtcgccg
      661 atcgacaggc tggccgcacc ttcgccgaag aactcgacgt aggcacccac cacgcgctcc
      721 ttgcgcagga actcggtcag cgccagcacg atgtcggtgc aggtgatgcc gggctggtgc
      781 ttgccgctca gcttgacgcc gacgatgtcg ggcaggcgca tcatcgaggc gcggccgagc
      841 atcacggttt cggcttccag gccgccgacg ccgatagcga tcacgccgag ggcatcgacg
      901 tgcggggtgt ggctgtcggt gccgacgcag gtatccggga aggcgacgcc gccacgggcc
      961 tggatcaccg gcgacatctt ctccaggttg atctggtgca tgatgccgtt gccggccggg
     1021 atcacgtcga cgttcttgaa cgcggtcttg gtccactcga tgaagtggaa gcggtcctcg
     1081 ttgcggcggt cttcgatggc gcggttcttg gcgaaggcgt ccggatcgaa gcccggggct
     1141 tccacggcca gcgagtggtc gacgatcagc tgggtcggca ccaccgggtt gaccttggcc
     1201 gggtcgccgc cctgctcggc aatcgcatcg cgcaggccgg ccaggtcgac cagcgcggtc
     1261 tggccgagga tgtcgtggca gaccacgcgg gccgggtacc aggggaagtc caggtcgcgc
     1321 ttgcgctcga tcagctgctt gagcgagtcg gtcagcatgg ccggatcgca gcggcgcacc
     1381 agctgctcgg ccagcacgcg ggaggtgtac ggcagcttgg cgtaggcgcc cggggcgatg
     1441 gcatccaccg cctcgcgcac gtcgaagtag tccagctgag tgccggccag ggatttgcgg
     1501 tattgggtgt tcatcttatt tgcgatcctt gagcggcacg aacttcaggt cctcgggacc
     1561 gacgtagttg gcgctcgggc gaatgatctt gccgtcgaca cgctgctcga tgacgtggga
     1621 cgaccagccg gaggtgcggg cgatcacgaa cagcggggtg aacatggcgg tgggcacgcc
     1681 catcatgtgg tagctgacgg cgctgaacca atccaggttg gggaacatct tcttgatttc
     1741 ccacatcacg ctttcgaggc gctcggcgat gtcgaacatc ttggtgctgc cctgctcggc
     1801 gctgagttcg cgagcgactt ccttgatcac cttgttgcgc gggtcggcca cggtgtagac
     1861 cgggtgaccg aagccgatca ccacttcctt cctctcgacg cgggcacgga tgtcggcctc
     1921 ggcctcgtcc ggattgtcgt agcgcttctg gatctcgaag gccacttcgt tggcgccgcc
     1981 atgcttgggc ccgcgcagcg caccgatggc gccggtgatg caggagaaca tgtcggaacc
     2041 ggtgccggcg atcacccggg aggtgaaggt ggaggcgttg aattcgtgct cggcatacag
     2101 gttcagcgag gtgtgcatgg cgcgcaccca ggagtcgcgc ggcttcacgc cgtgcagcag
     2161 gtgcaggaag tggccgccga tggaatcgtc gtcggtttcc acctcgatgc gcttgccgtt
     2221 gtggctgaag tggtaccagt acagcagcat ggagcccagc gacgccatca gcttgtcggc
     2281 gatgtcacga gcgcccgggt ggttgtgatc gtccttctcc ggcgacaggc agccgagcac
     2341 cgaggtgccg gtgcgcatca cgtccatcgg gtgggcggag ggcggcagct gctcgagagc
     2401 ggctttcacg ccagccggca ggccgcgcag ggacttcagc ttggtcttgt agctggccag
     2461 ttcggcgacg ttcggcagct tgccatgcac cagcaggtgg gcgatctcct cgaactcgca
     2521 ggtggaggcg acgtcgagaa tgtcatagcc gcggtagtgc agatcgttgc cgctgcgacc
     2581 cacggtgcac agggcggtat tgccggcagt ggtgccgctc agggcgacgg atttcttcgg
     2641 cttgaagcca acggtggtgg tttctacggt agcggtcatc gctggtatct cctaatccgg
     2701 ttggatattg ctcttgtttt cttcccctgg ccggccggcg ggccggccag ggtgccggtc
     2761 acttcttggc ggcgaacagc gcgtcgagct tctgctcgaa ggcgtggtag ccgatgcgat
     2821 cgtacagctc gacgcgggtc tgcatcaggt cgatgacttc tttctggtga ccgtcgcggc
     2881 gaatggcggt gtagacggcc tcggcggcct tgttggcggc acggaaggcg gacagcggat
     2941 agagctggat ggccacgccg accgaggcca gctcatcgcg cgagaacagc ggggtggcgc
     3001 cgaactcggt gatgttggcc agcaccggca cgttcagcgc gtcgacgaaa cgcttgtagg
     3061 tcggcaggtc gtaggcggcc tcggcgaaga tggcgtcggc gccagcttcc acgtagcgca
     3121 ggcaacgctc gatggcggcg tccacaccct cggcctggat ggcgtcggtg cgcgcgatca
     3181 ggaagaagtt cgggtcggtc ttggcgtcgg cggcagcctt cacgcggtcg gccatctcct
     3241 cggtggagac gatttccttg cccggacggt ggccgcaacg cttggcgccg acctggtcct
     3301 cgatgtgcgc ggcggcggca ccggccttga tcaggctctt gacggtacgc tcgatgttga
     3361 aggcgctcgg accgaagccg gtgtcgatgt ccaccagcag cggcaggtcg cagacgtcgg
     3421 tgatgcggcg cacgtcggtg agcacatcat ccagggtgtt gatgcccagg tccggcaggc
     3481 ccagcgagcc ggcggccacg ccgccaccgg acaggtagat cgccttgaag cctgcgcgtt
     3541 tggccagcag cgcgtggttg gcgttgatcg cgccgatcac ttgcagcgga tgctcgttgg
     3601 cgatggcttc gcggaacagc tgaccgggag tcttgttact catgacttac ctcgttgcgt
     3661 gacgtgcttg tgggtagcgc cctggtagtg acgcgcaatg tttttcttgg atgcggcgat
     3721 atgacggcgc atcagcaact cggccagttc gccgtcacgg gcggcgatgg catcgagaat
     3781 gcggtggtgc tcggtgaaag cctgctgcgg acggttcggc gtggcggaga actggatgcg
     3841 gtacatgcgc accagctgat acagctcgtt gcacagcaga ttgctcaggg tgcggttgcc
     3901 gctgccctgg atgatccggt aatggaagtc gaaatcgcct tcctgctggt agtagccgcg
     3961 cccggcccgg aacgcctcgt cgctctggtg ggtttccagc acatggcgca ggtcgtcgat
     4021 ctccgcttgg ctcatgcgct cggcggccag gcggcaggcc atgccctcca gcgactcgcg
     4081 gatctcgtac agttcgacca gttcggcctg ggtcagcgac accacccgcg ccccgacatg
     4141 cggcacgcgc accagcaagc gctgcccttc caggcgacgg atggcctcgc gcaacggacc
     4201 ccggctgatg ccataggcgc gcgccagctc gggctcggag atcttgctac cgggagcgat
     4261 gtcgccacgc acgatggccg actggatacg ctggaacaca tgatccgaga gcgtaccaac
     4321 ctcttcgtct acaatttggg tttctgcagc agcttccatc atattgtcga cacattggtt
     4381 gagcgaatga caaaaaacta cgactcactg ctgtaaatgt caaaccccct tagacgattg
     4441 tcgacaatcg gccggaaagc gccaaccctg ccacaactct ggcacgccac cggagcaacg
     4501 ccgcagcctt cgcccgctag cgcgccccga ctgtcgccgg accactggtc gcatgctaga
     4561 atccgggccg cccagcgctt gcagagcccg cgatgacgcc ccgcgcgctc tcccccgcaa
     4621 ttgctgcgcc cccatcacgc cttatcgaac gggacccatg agactcaagc cactcgtcct
     4681 gctactcagc ctgctgctgc cctgcggcct tgccctcgcg gcggaaaaaa ccgtttacgg
     4741 cctgcacgaa tatgcccgcc tgcaggaact cggcacggtt ctggctgcca aactcgacac
     4801 cggcgccaag accgcctcgc tcagcgccca caacattaag cgcttcaagc gcaatggcga
     4861 gccttgggtg cgcttcaccc ccggcagcga tgaaaacgac aacacggtga tcgagaaacc
     4921 cctggcgcga atcagcaaga tcaagcggcg ggctggtgac ttcgatcccg atgaaggcaa
     4981 gacctacacc gcaagacccg taatcgaact ggatgtctgc atgggcactg cgttacgcac
     5041 catcgaagtc aacctcaccg accgcagcgc cttcgaatat ccgctcctga tcggctccga
     5101 cgccctcaag cgtttcggcg ccctggtcga ccccagcctc aagtacgccg ccggccaacc
     5161 cggctgccct aacgttgcac cctccgacga gtaaagttga tgcgctctct gacactgcac
     5221 ctgaaagtcc tcatcgccat actggtgagc ctgggcgtgg cgatcactgc ctaccagatt
     5281 ttcgtcctcg gcattccagt caccgaggcg gaaacggata acgtctggag catcgatgcc
     5341 aaggtcgagt tccaggccaa tccgcgcaac cccatcaagg tgcagatgtt cgtgccgccg
     5401 ctgggccagg acttcgtcag cctcaacgag agcttcatct ccaacaacta cggcgtcagc
     5461 gtcaacaatg tcgacggcaa ccgccgggtg acctggtcgg cccgccgtgc caatggcccg
     5521 cagaccctct actaccgcat ggcgctgacc cagcgcttca cggcggaaaa gcccaaggaa
     5581 gaaggcccga tctaccgtga aggcctgccg ctggccggcc ccgagcaggt cgccgccgaa
     5641 gccctgctgg cgcccatccg ccagcactcg gccgacgtcg agaccttcat cagcgagacc
     5701 atcaagcgcg tcaacaacct caatgacgac aacgtcaagc tgctgctggc cggcgacacc
     5761 tcggtcgaga agcgtgcccg cgtgatcgac ctgctgctgt ccatcggcca cgtgccgctg
     5821 gagcaggtcc acaccatccg cctgatcgaa aaccagccgc agactcccga gctctggctg
     5881 cgcagcttca acggcgagaa ctggctgtac ttcaacccgg acaacggcca gcagggcctg
     5941 ccgcgcgacc gcctgatctg gtggctgggc gacgccccgc tgctcagcgt cgacggcggc
     6001 aagcaggccc aggtcaactt caccctcaac accagcgaac tgaatgccat cagcctggcc
     6061 aagcagtccg acgcctcggt tgattccggc ttcctcgaat actcgctgta cggcctgccg
     6121 ctgcagaccc agcagaccta ccagatcatg atcatgatcc cgatcggcgt gctggtgatc
     6181 ctgatcctgc gcaacctcgg cggcctgcag accctcggca ccttcacccc ggtgctgatc
     6241 gccctcgcct tccgcgagac ccagctgggc ttcggcatca tgctgttcac catcatcacc
     6301 gcgctgggcc tgtcgctgcg ctcctacctc gagcacctca agctgcagat gctgccgcgc
     6361 ctgtcggtgg tgctgacctt cgtggtggtg ctgatcgcgg tgatcagcct gttcagccac
     6421 aagctcggcc tcgaacgcgg cctgtcggtg gcgctgttcc cgatggtgat cctgaccatg
     6481 accatcgagc gcctgtcgat cacctgggaa gagcgtggcg gcggccatgc cttcaaggtc
     6541 gccgtcggca ccctgatcgc cgccaccctg gcctacctgc tgatgaccat tccggagctg
     6601 acctacttca tcttcacctt cccggcggtg ctgctgatcc tggtgggctt catgctggcg
     6661 atgggtcgct accgcggcta ccgcctgacc gagctgttcc gcttcaaagc cttcctcaag
     6721 gactgatgcc atgttcggtc tgatcaagag atggaaggcc ctgagtgcca agggcatcat
     6781 gggcatcaac cggcgcaacg cggactacgt gctgaagtac aacaagcgca gtctgtaccc
     6841 ggtggtggac gacaagatcc tcaccaagga acgcgccatc aaggccggca tccacgtgcc
     6901 ggagatgtac gggatcatct ccacggagaa ggagatcgag aacctcgaca agatcatcgg
     6961 cgagcacaac gacttcgtga tcaagccggc gcagggcgcc ggcggtgacg gcatcctggt
     7021 gatcgccgac cgcttcgagg gccgcttcaa gaccgtctcc ggcaagatcg tcagccgcga
     7081 cgagatcgaa cagcagatct ccagcatcct ctccggcctc tactcgctgg gcggcggccg
     7141 cgaccgcgcg ctgatcgaat accgggtcac cccggacccg atcttcaaga gcatcagcta
     7201 cgaaggcgtg ccggacatcc gcatcatcgt gctgatgggc tacccggtga tggccatgct
     7261 gcgcctgccg acccggcagt cgggcggcaa ggccaacctg caccagggcg ccatcggcgt
     7321 cggcgtcgac ctggccaccg gcctcaccct gcgcggcacc tggctgaaca acaagatcac
     7381 caagcacccg gacaccacca acgcggtgga tggtgtgcaa ctgccgaact gggacggctt
     7441 catgaagctg gccgccggtt gctacgaact gtgcgggctg ggctacatcg gcgtcgacat
     7501 ggtgctggac gtggaaaagg gcccgctgat cctcgaactc aacgcccgcc cgggcctgaa
     7561 catccagatc gccaacgact gtggcctgac tcaccgcgcc catgcggtgg aaacccgcct
     7621 ggaagagctg aaggccaagg gcatccagga aactccggag cagcgcgtgc gcttctccca
     7681 ggagctgttc ggccacgtgc aaccggccga attctgagcc cgcagtgggc gcccagcggt
     7741 cgggcgccca ctgctttccg tcgttccccc gccaccacga cccaatccct ctaggctgcg
     7801 gcttgccggc gctctagaat cccggcttgc gatagtccga gccaagccat gtccatctgc
     7861 cgcgtttacc ccctgcccta ccatgccgac ccgcgttcgc gcttcgccct cgtgcgcgca
     7921 gcgcccggcg cctgcctgct ggacggcggt caaccggcag ccgaacgggg ccgctacgac
     7981 ctgttcagcg cctggccgga acggcacctg caagcgcagg ccggcgagga cggccgccag
     8041 tttctcgccc gctgtcgcgt cgcgctggcc gaactgggcc gcgccgaagc cccggacgac
     8101 tgcgagctgc cgttcaccgg cggcctgctc ggctacctcg gctacgactt cggccgccgc
     8161 ctggagcgcc tgccgcagca cgccgccgcc gacctggcgc tgccggatgc cgcgttcggc
     8221 gtctacgcct gggcgctggt cagcgaccac cagcgccgga cctcgcagct ggtgttccac
     8281 ccgagcctgg cggagccgcg ccgacaggcg ctgatcgcgc tgttcaccgg cgtgcagccg
     8341 gctgccgcgc ccgggccgtt ccgtctgtcc cagccgttcc aggccgacct cagcgccgcg
     8401 cagtatcgcc aggcgatcga gaagatccag gcctacattc gcgccggcga ctgctaccag
     8461 gtcaacttcg cccagcgttt ccaggcgccc tggcagggcg atccgtggcg cgcctacctg
     8521 gccctgcgcg acgcctgtcc gacacccttc gccggcttcc tgcgactcga ccggggcacc
     8581 ctgctcagcc tgtccccgga acgcttcatc cgcgtcagcc gggggcaggt ggagacccgg
     8641 ccgatcaagg gcacccggcc gcgcggccgc aatagcgagg aagaccgggc gctggccgag
     8701 gaactgctgg acagccgcaa ggaccgcgcg gaaaacctga tgatcgtcga cctgctgcgc
     8761 aacgacctcg gccgcagctg ccgcatcggt tcggtgcgcg tgccggagct gttcgccctg
     8821 gagagctacc ccaacgtgca ccacctggtc agcgcggtga ccggcgagct ggcgcccggc
     8881 ctggatgcct tcgacctgct ggccggcagc ttccccggcg gctcgatcac cggcgcgccg
     8941 aagatccgcg ccatgcagat catcgacgaa ctggaaccga cccgccgctc gatctactgc
     9001 ggctcgctgc tgtacatcga cagccgcggc gagatggaca gctccatctg catccgcacc
     9061 ctgctgctgc aggacggcca ggccagctgc tggggaggcg gcggcatcgt catggactcc
     9121 gagtggcagg cggaatacga ggagtcgaag accaaggtac gggtgctgct acagacgctg
     9181 gaagccaccc tcgactgaat acccatgcgc ggccgcaaag gcgaccgggg cgacactccc
     9241 cgtcgcgccg actgtctaca ctgcgccccg gccccgcttc gttgcttaga aaggatcccc
     9301 atgcccagcc ttaaccgcat catcctgatc aacacccacc tgcagggcgt ggtggaactg
     9361 gtcgtggatg accacaccaa catctgcggc accaacgcct ccggcaagac caccctgcaa
     9421 cgcctggtgc cggtgttcta cggcgaatac ccgagccgcg tggtgccggc cacccgcgac
     9481 agcttcgagc gctggtacct gcccaccgag cagagcttca tcgtctacga ataccggcgc
     9541 atggacgacc agctgtgcca ggcggtgctg gccgccgcca gcgacggcaa gggcgtcgcc
     9601 tatcgcctgg tgcacaaggc cttcgcgctg gacgactaca tcaagagccg ccagggcgat
     9661 gcgatcacct gcctgaacat ggccgaactg ggccgccagc tgaagcaggc cggcgtgccg
     9721 gtcagcaccc tgctcaacac ccgccagttc cgcgccatca tccagaacga ccgcagcctg
     9781 ctcgccgccg acggccagcg cgctgagctg cgcagctggg cgcgccagtt ctccctgtgc
     9841 gacgccgagc actcgctgcg ccatatcgag aaactggccc gcgccgtgca ttcgcgcgag
     9901 ggcaagatgg agaccgtcaa gtcgatggtc gccgccatcc tcgaggagga cggtgtcagc
     9961 ccgccggcca cccatctcaa cctgcagcgc gtcgaggact ggatccgcga cagccggctg
    10021 atccagggct tcaccgccat ccgcccggag ttcgacaagc tggagcgcga ataccaggac
    10081 ctgctcgcct gcgaggcgcg cctgggcggc ctgctgctgg gctaccgcgc cgacgagccg
    10141 cgcctctacc agcgccagga ggaaaccacc gcggtcatcg agcagaccgg cttccgcctc
    10201 ggccagctgg aggagcgctg gaaggaccag cgcgacaccc tcaaccagga cctgtcggcg
    10261 gccaagggcg acatcggccg catcggcgac gaactggacc agatcgacaa ccagtaccaa
    10321 accttcctcg acgccgacat cgagcaggcc aaggccgacc tggagcagct cggcgcctgg
    10381 cgcgacgacc tgggcaacct gcaggcccgc caccgcctgc tgaccgacca gcatcaggac
    10441 gtggagcgcg cctacctgga gcgccagcag gcgatcaagg accagcgcga agcggcgctg
    10501 gagcttctgc gcggccagga ggatgcgctg cgcgaggagc gcgacggcca gaaggcgcag
    10561 caggacgagg cgctgggcgc cctggagaag gccttcgccg cccgccgcca gcagggccag
    10621 gaggacttca cccagcgcga atacgctctg aagctggagc gggggcgcct ggagcagcag
    10681 atcgccagcg tcggctacag cgaggaggaa aatctcgccc tggccattct cgaccgccgc
    10741 ctcgaagagg ccgacgagaa gggcgaggcg gccgaggcca gggtccgcca gctggaaggc
    10801 caggagcgcc agctgcgcag ccgccgcgac gaggccaaca cggcgttcgc gctggccgcc
    10861 cggcgggcgg ccgagcgcca ggagcagctg gaggatctcg agcgcgcgct ctacccgggc
    10921 cagcacaccc tgctggaatt cctgcgccgc gagcagcccg gctgggaggc gcggctcggc
    10981 aaggtgatcc atccggcgct gctgcagcgc agcgatctca agccggcggt ggtcgatgtc
    11041 gatccggaca gcctgttcgg cctgcagctg gacctggccg ccctcgacat ccccgagcat
    11101 gcggccagcg agcaggagct gcgccagcgc gtccagctgg ccaaggacgc cctgcaggac
    11161 gcccgcaacc agcagcagga ggcggaaagc cggctggtcg aagccagcga agccctcgac
    11221 gagctgcagc gccagctggt ggtggcgcgc agcgaggcac agaaccgccg cgacgaccgc
    11281 cagcgcctca aggaggagcg ccgcagccag aaggacaagc tggatgccgc ggtgcaggag
    11341 cgtcgctcgc aggcgcgcaa gcagctggtc gagctcgaca acgccctcaa gcagctcgcc
    11401 agcgcgcgcg acggctggct gagcgagctg aaggcgcagc acagcgagga gcgcctggag
    11461 acctccgccc actggcagca ggtgatcggt agtctcgaca gccagatccg ccagctgcgg
    11521 gccgccgccg acgagcgccg ggccaatgcc caggccgaac tggcccagtg cgagcactgg
    11581 taccaggccg agctgaagtc ccgcggcgtc gacgaaaccg tcatcatcga gctgaaacgg
    11641 cagatcaagg gcctcaccga caggatcgcc cgcaccgaac aacgccgcgc cgacgtcctg
    11701 cgctacgacg attggtatcg ccacagctgg ctgcaacgca agccgaagct gcaggccgaa
    11761 ctgggcgaca gccgcgcccg cgccgccact ctggagcagc agctgaagac cgcctcggcc
    11821 gagtacaagg cggaacgcag ccagctggaa acaacccgcc agcaggccct gcagcagaag
    11881 ggcgctctcg acgacgccct gacccgcctg aaagccctgc tgcgccgcct cggcgagctg
    11941 aaactgtcgc gcgacggcga gatcgccgag ggcgaactgg acgagcgcct gcgcctgggc
    12001 gaggaagtgc tgcacggccg cgagcagatg ctcgccgcca tcaaggccca cgtggagcac
    12061 ttcgacagcc tgatcgtcgg ccagtccggc tccagcctgg cggaaacctg ggagcgcagc
    12121 cgcgaggaat gcaccctggc caacgagcgc ggcatcccca gcctcgacta ccgtcgcctg
    12181 gtgccgcacc tggcgcagct gctcaacgtg atggtgccgc agaaactcac cggcctgcgc
    12241 gagcagggca agatcttcgg cctcgacctg catggctact acgaggtgct ggccgacatc
    12301 gaccggcgca tcgccacgca gagcgcgcgg atcacccgcg aggtcagcga ggaactgttc
    12361 ctcgacggcg tctccgagtc ggcggtgcgc atccgctcgc ggatcagcga gctggagttc
    12421 tggccggagc tgaaggcctt catcaaggcg ttccgcgagt ggcgggaagc cggcttcagc
    12481 gaactgcccg gcgaggagta caccaccagc atgcgccgcg cgctggacat catcggccgc
    12541 tcggcgctga gcggcggcat cgccgccctg ctggaaatcg agctgcgcct gcgcgaaggc
    12601 aacagcgacc tggtgatccg caccgaccgc cagctcaacg aatcctccag ccacggcatg
    12661 gcctacctga tcctctgcaa gttcctgctc gccttcaccc gcctgctgcg cggcgacgcg
    12721 ccggccacca tccactggcc gatcgacgaa ctgggcaccc tgcaccacaa caacgtgaag
    12781 aagatcttcg acgcctgctc aagcaacaac atccgcatcc tcggcgcctt ccccaacccg
    12841 gactccgagg tgctgtcgct gttcgccaac cgctacatcg tcaacaagca gacccgccag
    12901 ctgcaggtgg tcaagccgca gctggatgtc atcgccgaaa aactcaaagg gctgaaaacc
    12961 ggggaggtcg cctaaatgct ctcgacgaag ggacaggtca tcgagcgtct gctcaagggc
    13021 gacttcatct gccgcaccac cgacgaggac ggctggcgct tcctgaagac gccggccaac
    13081 cgcgagcagg tggacgacta cctggccacg ctcaaccgca tggtggccac ggtcggcagc
    13141 ggcgccgaca gcgacgtgtt cttctgcggc taccgccagc tcgacgagga cgagcgcaag
    13201 gtcgtcggcc agcagttccg cgatgtctgc aatgccctca cgccgctggt cgaatggctg
    13261 gtgctggtgc aggaagccgg cgcccaggat gcgccgctca ccgaaggcag cgccctgcgc
    13321 ctcaacgagc tgcagagcat cgtcgaggac accccggcgt tccgcgagca actggcacgg
    13381 atcagccact accggctgtt cggctccacc agcgccaccg tcgacggcca gatcaagcag
    13441 gtgttcaagc gcctgtgcga gctgggctac ctggtgcggc ccaacccgga caagcagatc
    13501 tacctcggca ccggcaagct ggactatctg tacgaggtga tccgcttcat cgacgaaacc
    13561 gagagcctcg atctggaaga gcgcgccgaa agcgccagcc agggcagcct gctatgagcg
    13621 gcaacctgca tcaggccggg gtgcgcttcc tgcgccaact gggccggcat gccgaaccga
    13681 tcatggacgc ctacctggcc ggctccttgc ccgaccaggc gctggagccg gcggtgcagg
    13741 agcgcttggt caaggacggc atcctctacc gccccgaacc cggcgccgac ctgcatttgc
    13801 gccgggtggt gcgcgcgctg ctggaggagg ccctgcgcga cgaccgcaac cggcagatcg
    13861 acgccaacac cggcagcgca ctggtcacct tcaagaccct cgctgcgcac tacaaggaag
    13921 cccgtcacca gggcgactac gccgcggcgg acgcctacct ggcggacctc aaggagcatg
    13981 tctacgcctt cggcgaaacc ctgagccacg gcatccgcgt gctgtggagc cggatcaaca
    14041 acgagttcgg ctacgtcggc accctcaacg ccaagatccg cgagaacgaa ctggcccaat
    14101 cgcaggtgag cgaactgctc gccgggctgg aaatgatcag cttcgcggag ctggccgaca
    14161 ccgccggcga cctgcgcgaa ctgcgccgcc tgctggtcac cagcctgcag cgcacggtca
    14221 gcgcctgctc ccaggagctg agcatcgtcc agggtcgcct gctggagctg ctcggccgct
    14281 tccggcagat ccgcggccgc acccgtctgc tcaagggctg gctgctgcac atggagcagc
    14341 agccggacta ccaggtcggc aaccatgccg cccaccagaa cgtgccgcag ctgttcaacc
    14401 aggccgcggc ggtcctcgcg ccggccgccg tggacatcca caacccgctg cacgaggcgc
    14461 aactgctcgc gctggtggcg cagatccgcg ccctgcagcc gccggaccgc ttcgcccccg
    14521 cgccggccga ggccggcgag ttcgtgcttg gcgaggtgga ggccttcgag atcgagcaca
    14581 acccgatccg cgaggcggta tcggcctact tctgccaggt gatcgacagc ggccaaccga
    14641 tctcggcgct ggcctggcgg gcgcaacagg cgctgccctg ggatgcggaa agctggctgt
    14701 accaggtgat cggcggctac gagggcctgc cggaagagca gaagcgccac ttcgagctgg
    14761 atccgatcgg cgaggcgcat ccgctgtact ccggcaactt catcgtgcgc gacgtgcggc
    14821 tgtggctggc ctgagccgca tccatcagca ggcggtgcac gacctgctgc gcgggcgcct
    14881 ggagcgggtc gcactgaacg cgacctggcg ccgaatccag gtccacttcg aggtcggtac
    14941 atttgacggc cgctggctgc agttcgaccg ccagcaacgg gagatcctgc gccagctggc
    15001 gcagcaggag tggggtttcg atccgctgga aggtgtgccg gacggcacac gcctggaggt
    15061 tgccggccag gccatcgacg agaagaccgc ccgccagcgc cccgacgata gccacgtgct
    15121 ggtcaagggc gccctgcccg ccccgctgcc gacgctgccg ccggaactga gcctgcgcgt
    15181 gccgctggcc agcctggacc tcgccgccat cacccaggtg ctggtgatcg agaacctcga
    15241 cagcttcgac gactggcagc actacgtcgc accgattgag ctggccggca gtctgctgct
    15301 ctatcgcggc cacggcggcc tggcccgcgg cgtgcatcaa ctgctgaaca gcctacccgc
    15361 cagcacacgg gtaatagtgt tccccgacta cgatcctgcc ggcttgaaaa tcgccgcgac
    15421 atcgccccgc gccactgcgc tgctgctgcc cgaaccggac aagcacctgc tggccaaggg
    15481 cagccggcaa cacttcgacg aacagttcca ggaagcacgc tatctggatg gcgcggagct
    15541 gggcagttgg cagcccgtgt gggacgagat gaaggcgcgg caggtcagca tcaagcagca
    15601 gcatatgctg gcattgggcg tgcgactgag gatagtgccg cgccaccccc gctgattgct
    15661 caccgcccat aaacaccgag gcccgcacag tggcgggcct cggtcttgct acggcagcgc
    15721 tcgcttacag gctgaggtcg cgcaccgacg ccttgaggaa ctcctgcttg agatcctcgt
    15781 aggtgtgtac cgccgggaac tgcgggaact cgcggatcac gttttccggc gcgtggaaca
    15841 ggatgccgga gtgggcttcg ccgagcatgg tggtgtcgtt gtacgagtcg ccggcggcga
    15901 tcacgcggta gtacaggctc ttcagggcga tgaccgactg acgcttggga tccttctggc
    15961 gcagctggta gtccaccacg cggtcggtct cgtcggtgat cagcttgtgg cacagcaggg
    16021 tcgggaaacc gagctggcgc atcagcggct gggaaaactc gtagaaggtg tcggagagga
    16081 tcaccacctg gaagcgctcg cgcagccagt cgacgaactc caccgcgccg tccagcggct
    16141 tcagggtggc gatcacttcc tggatatcgc gcagcttcag gccgtgctcg tcgaggatgc
    16201 gcagacgctg cttcatcagc acgtcatagt cgggaatgtc gcgggtagtc gccttgagcg
    16261 cgtcgattcc ggttttttcg gcgaaggcga tccagatttc cgggaccagt acaccctcga
    16321 gatcaaggca ggcgatttcc acagggcatt cctcatttat cagccagtga cggaggcggc
    16381 actctagcga cttgcctgcc ggggtgcaac gcagcgcgcg ccggcgcgcc gaaaaagcag
    16441 gatggaagcg accagagccg caccgaccac atattcccaa ttgttagcag gtcatggtga
    16501 tttggttatt tcagcgtata aacgccttgc tgttaccatc gcggctacgc atcagccacg
    16561 aatctaccgg acaccccgat gagccagacc ttcgatgtcg ccgccctggc ggccgcctac
    16621 gccgacaaat ccccgcagga ggtgctcaag ctcgccttcg aacacttcgg cgacgagctg
    16681 tggatctcct tcagcggcgc cgaggacgtg gtgctggtgg acatggcctg gaagctgaac
    16741 aagaacgtca aggtgttcag cctggatacc ggccgcctgc acgcgcagac ctaccgcttc
    16801 atcgagcagg tgcgcgaaca ctacggcatc cagatcgagg tgctctcccc cgacccacgc
    16861 ctgctcgagc cgctggtgcg cgagaagggc ctgttcagct tctacaagga cggccacggc
    16921 gagtgctgcg gcatccgcaa gatcgagcca ctcaagcgca agctggccac cgtgaaggcc
    16981 tgggccaccg gccagcgccg cgaccagagc cccggcaccc gcagccaggt ggccgtgctc
    17041 gaagtggacg gcgccttctc cacgccggag cagccgctgt acaagttcaa tccgctggcc
    17101 aacatgagca gcgaggaagt gtggggctac atccgcatgc tggagatccc ctacaacccg
    17161 ctgcacgagc agggcttcat cagcatcggc tgcgagccct gcacccgccc ggtgctgccc
    17221 aaccagcacg agcgcgaggg gcgctggtgg tgggaggaag ccacccacaa ggaatgcggc
    17281 ctgcacgccg gcaacctgat cgccaagaac tgaaggcgag cccctgaaag cgaaaagccc
    17341 gacctggcga gtcgggcttt ctcattccag ccgggaaaac ctcagtggct gggcagcacg
    17401 acatccgcaa acagctcttc cagctcggcc ttgttgcggc actgcaccgc ctcggcgacc
    17461 cgctcgcgcg tcaggtgcgg cgcgaagcgc tcgatgaagt cgagcatgaa gccgcgcagg
    17521 aaggtgccgc ggcggaagcc gatcttggtc acgctggact cgaacagctc gctggcatcc
    17581 agcaccacca gatccttgtc cagctcagga tcgaccgcca tgccggcgac gatgccgacg
    17641 cccaggccca ggcgcacgta ggtcttgatc acgtcggcat cggcggccgt gaacaccacc
    17701 ttgggctcca gaccgcgatg gctgaacgcc tcgtccagct tggagcggcc ggtgaagccg
    17761 aacacgtagg tgacgatcgg ctgctcggcc agcgcctcga gggtcagctt gggcaggcgg
    17821 gtcagcgggt gcccctgcgg cacgatcacg cagcggttcc agcgatagca gggcatcatc
    17881 accagatcgc cgaactgctc cagcgcctcg gtggcgatgg cgaaatccac ggtgccgtcg
    17941 gccgccattt cggcgatctg cgatggcgag ccctggtgca tgtgcagggc cacctcggga
    18001 tactgcttga tgaaggcgct gatcacttcc ggcagggcat agcgcgcctg ggtgtgggtg
    18061 gtggcgatcg acagggtgcc cttcttctcg ttggagaact cctgggcgat ctgcttgatg
    18121 ctctccacct tgcgcaggat ctcgccggcc gtggtgatga tccgttcgcc ggccggggta
    18181 acgcgggtca ggtgcttgcc gctgcgggcg aacacctcga cgcccagctc gtcctcgagc
    18241 aggcggatct gcttgctgat ccctggctgc gaggtgtaga ggctctgcgc cgtggccgaa
    18301 acattcaggt catgatgcgc cacttcccag atgtaacgca gttgctgaag cttcatgagt
    18361 gatcctcaaa gcagaaattg gtcggacggc tgaccagtgg cggccgcctc ttataaccac
    18421 aatagactat attttttcat attagacgaa aaatgttgtt ttgtacgcgc atcactgcag
    18481 cgcgggctct tccggcagga taggcaccag atagacgggg acacgcccct gctgcagcag
    18541 acgcaggcag gtacgcccca gcgccgaaca ggcgggcatg ctctggctgt gcgagccgat
    18601 cacgatcagg tcgaccgcca ggtcctgggc ttcctgcagg atcacctgcg gcgggtcgcc
    18661 gagctgaacc cgcaccgcac ggatcggcac actttccccg tcgctttccc gcaattcgtc
    18721 acagaagcct tcgaacaccc gctgctcgat gctcgccatc acgctgtcca gcccgcgcag
    18781 gcgcagatcg ttgtagctgt cgctatccag ataggtctgc agcaccgact cggcgaacag
    18841 gccgagcggc tcgaccacat gcaggacata gagatcggcg cgatgggtac gcgccagcaa
    18901 cagcgcatgc tgcagcacgt agggggcaaa caggccgagg tcggtggcgt agaggatgga
    18961 acggatcatc aaacctccgc tggcgaggtt tttgcagttc tccctcagcg tagcaggcgt
    19021 ttatccgctc actcgctggc cacgccgtgc ggtacatggc cactgggaac agtggccagc
    19081 ctgatgtcgg ttgccgtcct attgctgcgc cgcctgcagg aaagccaggg cctgatacag
    19141 catgcgcacc cgcggcatgt cgacacccgc cgccgccgcg gctgccagtg gcgcagcata
    19201 cagcgccggc agttcggcct cccgccgctg ggcgaagtcg tggtacatgc tcggcaggta
    19261 gtcgggcatg cggtcggtgt aggccagcag cttctgcgca tagttatccg gcaggctgta
    19321 gccgcaggcc agggtggcat ccaccacctc ctgcatcagt gcctgcagca gcgcacgact
    19381 gtccgggttg gccatcaacg gccgggtgga actgtgcagc agcaccgaca ggccgttgta
    19441 cggcacgttc cacaccagct tctgccagcg cgcctggttc aggtcggcga tcacgctgga
    19501 ctcgatgccc gcctcgcgca gccagctcac gccctgctcg agcagcgcct ggcgctcctc
    19561 tgcagtcgcc gggccgctgt gatagccgag attgcacacg ccctgcgcct gatgcaccac
    19621 cacccccggc gcctcgcgat gggcgcagat gaagcacaag ccgccgagca ggtgcagtga
    19681 ctccggcagc agcggacgca gcgcgtcctc cactcccagg ccgttctgca gcagcaccac
    19741 cttcgccccg ggcgccgcca cctgcgcgat cagcggcgcc agctcgcggt tgctggtggt
    19801 cttggcgcca accagcagcc agtcgcaggc cggcatcgcg gccgcctcgc gccaggcgtt
    19861 gacgttgacg ttgggcagat gcaactcgcc gagctgggca tggcgcacgc gcagaccgtc
    19921 gcgggcgacc gccgcgtatt cgctgcgcag caggaaatgc acctcgaaac cggcacgcgc
    19981 cagcatcagg ccgtagtaac caccgatggc gccggtaccg atgatgccga tgcgcggacg
    20041 agcggatgcg ctcatggcaa tgctcctcaa caatagggat cgcgcactat cgacggccag
    20101 cgtcgacgca ggcaagaggc tcgcgcagaa aaccgcggcc agcatgccga tccggcccag
    20161 caaaaggcgc cggacggctg cttcaagccc ggaaaaactg caagtcttta ttgtgaagcg
    20221 gcggcctaaa gcgttaaggt tctggcatcc cgttgcccct atttgctgcg ctccgccacg
    20281 ggcctggctg gcggacggca tccgtgacct gacgagtagt aacacgatgg ctgatttacc
    20341 gatcgacgac cttaacatcg cctccaacga ggcgctgatc acccccgagc agctcaagca
    20401 ggaactcccc ctcaccgaaa cggcccgcca caccgtgagc catggccgcc aggtggtacg
    20461 cgacattctc gacggcaagg atcatcgcct gttcgtggtg gtcggcccct gctcgatcca
    20521 cgacatcaag gccgcccacg agtacgccga gcgcctcaag agcctggcag ccgaggtctc
    20581 cgacacccta tacctgatca tgcgcgtgta cttcgagaag ccgcgcacca ccgtcggctg
    20641 gaagggcctg atcaacgacc cgtacctcga cgactcgttc aagatccagg acggcctgca
    20701 catcggccgc aaactgctct gcgacctggc cgagatgggc ctgccgaccg ccaccgaagc
    20761 cctcgacccg atctccccgc agtacctgca ggacctgatc agctggtcgg ccatcggcgc
    20821 gcgtaccact gaatcgcaga cccaccgcga aatggcctcc ggcctgtcct cggcggtcgg
    20881 cttcaagaac ggcaccgacg gcggcctgac cgtggcgatc aacgccctgc agtcggtttc
    20941 cagcgcgcac cgcttcctcg gcctcaacca gcagggccag gtatcgatcg tcaccaccaa
    21001 gggcaaccgc tacggccacg tggtgttgcg cggcggcagt ggcaagccga actacgattc
    21061 ggtcagcgtc gccctgtgcg agcaggaact gaccaaggcc ggcatccgcc cgaacatcat
    21121 ggtcgactgc agccacgcca actccaacaa ggacccggcc ctgcagccgc tggtgatgga
    21181 aaacgtcgcc aaccagatcc tcgagggcaa caactcgatc atcggcctga tggtggaaag
    21241 caacctgggc tggggcagcc agtcgattcc gaagaacctc tgcgaactca agtacggcgt
    21301 gtccgtcacc gatgcctgca tcgactggga cagcacggaa aagaccctgc gcagcatgca
    21361 cgccaagctc aaggacgttc tgcccaagcg ccatcgcggc tgatcgcgcg ctggacacga
    21421 aaaagccggg catcagcccg gctttttctt tgcctcttgc acctcacgcc tggcgttcgc
    21481 cgttgcgctg gcggcgctcc aggtagcgct ccacgtagga gcaggatgga atgaccgtat
    21541 agccccgacc ttcggcgaac tgcagcgcct gctcggtcag cgctgccgcc accccgcgcc
    21601 cgcgcagggc atcgggaacg aaggtccggt agatatcgat ggtctgcttg cccaggtcca
    21661 tataggacaa gtaggcacgg taaccatcca ccgtcacctc gaattgctta cctgccagat
    21721 catgctgaat ggacagtggc tcgctcatca caactctctc cttacgctgc gcatcggtcg
    21781 caggtcgcct cgcgcaattg gacaaccgga ccccgggcaa gtgccatgcg ccaatcggaa
    21841 cagcgggcac gtgattattt tttaaacaat atcaatgtac taaatgcgga caaagacaaa
    21901 aatttcggcc tgccttgccc tcgaagcgtt tacttactag aactaatgcg tactatataa
    21961 cccagcagat ttccccgtga gggaaattgc atcacggaaa tccacccaaa ggggaacact
    22021 atgaacaacg ttctgaaaat ttccgctctg accctggctg ccatcctggc taccggttgc
    22081 agcagcatgt ccaaagaaac cgaagctcgc ctgaccgcta ccgaagatgc cgctgcccgc
    22141 gctcaagctc gtgctgacga agcctaccgc aaggccgacg aagctctggc cgctgctcag
    22201 aaggctcagc agaccgctga cgaagccaac gagcgcgctc tgcgtatgct ggaaaaagcc
    22261 agccgcaagt aataggtctt ccgacctgct atttgcagaa gccgacccgc aagggtcggc
    22321 ttttttattg cctgccgaaa ttgacctgca gtatgtactc cagcgcaaca atctaccggc
    22381 aataaaaaac ccgctgcagc aaaactgcaa cgggcttttc cggccagcga tggctagtgc
    22441 gcgaccacgc tggctacctg ctctcgcggg gcagcgatct ccaccggcaa accatcctcg
    22501 gcagccacca cttcgcgcac cacttcccag tccaggcgtg catcgtcctt gagatcctca
    22561 cgctggagca gcgcattgat cacctgggtg tgcttgtcga cgatcgacgg ctcgccgtgg
    22621 tcgtccagcg gcgtgtgtgc ttcaagatag atcttgccgc cacgtatgcc gaacttgtac
    22681 ggctcgttga tgatgcgcac cgacgtgccc accggcacaa tcttcgccaa cgccagcacg
    22741 ttgtggttga gcatgcggaa gcagccgtgg ctgacgcgca tgccgatgcc gaacctcttg
    22801 ttcgagccat ggatcaggta gccgggcgtg cccagggtga acttgaacgg ccccagcggg
    22861 ttgtccggcc ccgccggcac caccgccggc agcggatcac cctcggcagc atgctccttg
    22921 cgaatcgaag caggtggcgt ccaggtcgga ttcggcgtct tcgcggcgat gctggtacgg
    22981 gtgatcggcg acccccagcc ctcgcggccg atgcccagcg ggtaggtatg cacgacgttc
    23041 tgccccttgg ggaagtagta catgcggtat tcggccaggt tgatcacgat gccttcgcgc
    23101 ggccccggcg gcaggatgaa gcgggtcggc aggatgatct cggtatccac ccccggcagc
    23161 cagggatcga ctcccgggtt ggcggcgacc atctccagat agcccagatc gttggccgta
    23221 ccgatatcgg cgaaggtgtc ttcgtacttg gccttgatca cctggacttc gccgacgatg
    23281 tcctcgccgg gtggcggcag gggcagctcc agcgcgctgg caggtgcagc cagcagcagt
    23341 gcggccaggc tggccagcat ggggcgaacg gcaacaacac gcgagaacat cggtaatacc
    23401 tgaataaatt gaacgatcga ttattgtaca ccgccgccgc cggcaggcgc gagaatcagc
    23461 gcaccggcca gtcccgcggc agtccgtggc gctcggcgcg caggcgctgc agacaggtcg
    23521 agcacagccg gcggtccttg agcgaggccc tgtccagcac ctcccagagc ggatgcgccg
    23581 gcaacaggct gccgcacagg gtatggtcgc ccggatcgcc cagctccagc tgccgcgcga
    23641 ccaggtgcat gcgcacttcc tgacaggcga acaggtccag ttgctcgtcg ggaacgagca
    23701 agcggtaggc atacagggac caggattggc gcgacatcga gggctccggg gcggcgcggc
    23761 gcgcaacctt agccgaaagc ctcggacggg gaaagggtca gagcaggggc tgcagcgtcg
    23821 gccacacatt ttccagcagg atcgactgtg cttcggccgt cgggtggatg ccgtcggcct
    23881 ggatcagcgc acggacaccg ccgacgccct cgagcaagaa cggcaccagt gccacctgct
    23941 cctgctccgc cagctcggca tacacctgtt cgaaggcact actgtaacgc ggcccgtagt
    24001 tgggcggcag cttcatgccc agcagcagca cctgcgcccc ggcggcgcgg ctctgctcga
    24061 tcatcgccgc aagattctgt tgcaattgca gcggcggctg gccacgcaga ccatcgttgc
    24121 cacccagctc gatcagcacc agctgcggcg catgcgtctc gagcagcgcg ggcaggcggg
    24181 ccagcccgcc agcactggta tcgccgctca ccgaagcgtt caccacccgc cagtcggggg
    24241 cctcgcgctg caggcgcgag gccagcagat tgacccagcc gtcgcgggtt tccaggccga
    24301 aagccgcgct gatactatcg ccgacgacca gcaaggttcc cgccgccgca ttctgggcca
    24361 gcaacagcag tcccagacag ccgcgcaaca accatgtacg catcggattc tccatgagcg
    24421 ccagcattct taatgcgcga aaccttagca aagtggtcga cagcgcggaa ggcgcgttga
    24481 ccatcctcga cgacctcacc ctggagctga agcgcggcga cagcctggcc atcgtcggcc
    24541 gttccggttc gggcaaatcc accctgctcg gcctgctcgc cggcctcgac cagcccagca
    24601 gcggcgaagt gcagctggcc ggccacgccc tctcccgcct cgacgaggac cagcgcgccc
    24661 gcgtgcgcgc cgagcacgtc ggcttcgtgt tccagtcgtt ccagctgatc gacagcctca
    24721 ccgcgctgga gaacgtcatg ttgccgctgg aactggaggg ccgtgccgat gcccggcaga
    24781 gcgcacgcag cctgctcgaa cgggtcggcc tcggccagcg cctgagccac tacccgcgcc
    24841 agctctccgg cggcgaacag cagcgcgtgg cgatcgcccg cgcgttcgtc ggccaaccgg
    24901 acatcctgtt cgccgacgaa cccaccggca acctcgacag ccagaccggc gagcgcatca
    24961 gcgacctgct gttccaactc aaccacgagc acggcaccac tctggtactg gtcacccacg
    25021 acgcccgcct ggccgcacgc tgccagggcc agctgcgcct ggaaggtggc cgccgcgtgg
    25081 agacgcaggg ctgatgcgcg cgccttccct ggcccgcctg ctgcgcctga gcctgcgcca
    25141 gctgctgcgc gaggctcgcg ccggcgaact gcgcgtgctg ttcttcgccc tgctgatcgc
    25201 cgtggcggcg agcagcgcca tcggctactt cagcgcgcgc ctgaatggcg ccatgctgct
    25261 gcgcgccggc gagttcctcg ccgccgatct ggtgctgcgc ggcaccgccc cggccgcacc
    25321 ggagcagatc gccacc
//
LOCUS       DATCUN010000108         4722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_267492, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000108 DATCUN010000000
VERSION     DATCUN010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267492"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VIO83_04185"
     CDS             <1..513
                     /locus_tag="VIO83_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY8330507.1"
                     /translation="DELFCSRNFGPAIHKFGAILGGAFNFVDQNLFGGKIPLTLHDNK
                     PDYTCLKPAAESQKIDYPKPDGKLSFDKLSSVFLSNTNHEEDQPCHLKLADASIPLSK
                     NLPLYDEPAQRYCPAGVYEIVTQENGEKKFQINAQNCVHCKTCDIKDPAQNITWVAPE
                     GTGGPNYPNM"
     gene            complement(568..1137)
                     /locus_tag="VIO83_04190"
     CDS             complement(568..1137)
                     /locus_tag="VIO83_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEY8330508.1"
                     /translation="MNESDKAGDLLQQIPVTPGKGLPPVHLWNPPFCGDIDMRIARDG
                     SWFYMGTPIGRKPMVRLFSTILRRDGDDYFLVTPVEKVGIRVDDAPFVAVEVEVEGAG
                     EAQRLRFRTNVEDEVVAGGEHPLRVVLDAQTGEPSPYVHVRANLEALIHRNVFYRLVE
                     LLVPRQLDGQLWLGLWSGGEFFPVAPQPE"
     gene            1288..2415
                     /locus_tag="VIO83_04195"
     CDS             1288..2415
                     /locus_tag="VIO83_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8330509.1"
                     /translation="MTAWHDEFPQEPGLRYLNHAAVAPWPRRASQAVSAFAEQNMRVG
                     ARDYPQWLQLEQRLRTRLTRLLNAPSSADVALVKNTSEALSFVAFGLDWRSGDQVVIS
                     DQEFPSNRVVWEALRPQGVEVIEVSLAGSDPETALLAACTARTRLLAISAVQYASGLR
                     LDMTRLGAGCQQRGVLLCIDAIQQLGAQPFDVQASQCAFAMADGHKWMLGAEGLGVFY
                     CRRDLREQLKLHEYGWHMLEDVGNYDRRDWQPAQSARRFECGSPNMLGAVALEASLAL
                     LEEVGLAQVASLIEARIARLHAGIAALPGASLLSPPEPARRAGILTFSLAGWEHPRLF
                     EALKQQQVVCAQRGGGIRLSPHFYTPEQVIEETLELLRRLR"
     gene            complement(2676..3452)
                     /locus_tag="VIO83_04200"
     CDS             complement(2676..3452)
                     /locus_tag="VIO83_04200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY8330510.1"
                     /translation="MLVDSHCHLDRLDLAAHGGSLDAALAAARARGVGRFLCIGISAD
                     NAAAVRELAESHADVYCSIGVHPLDLEPGAEPALDWLLHELDHPRVVAIGETGLDYHY
                     QPEAATLQQASFRLHLEAAQQTGKPVIVHTREARQDTLDMLRAADLAQAGVLHCFTED
                     WDMAKAALDLGYYISLSGIVTFRNAEALREVARRIPADRLLVETDAPYLAPVPHRGKP
                     NLPEYVRDVAEFLAGLRGVAFETLAEQTTANFQRLFTLAV"
     gene            complement(3455..3808)
                     /locus_tag="VIO83_04205"
     CDS             complement(3455..3808)
                     /locus_tag="VIO83_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY8330511.1"
                     /translation="MSMPPLGGRNAILSLTLKDKAQLYAAYMPFVRNGGLFISTNKTY
                     RLGDEVFMLLSLMDEPEKIPVAGKVVWVTPKGAQSNRVAGVGVQLNDSDHTTRNKIET
                     YLAGALKSERPTLTM"
     gene            complement(3842..>4722)
                     /locus_tag="VIO83_04210"
     CDS             complement(3842..>4722)
                     /locus_tag="VIO83_04210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEY8330512.1"
                     /translation="AERLAAALLCSAPLATGAGACGQCKACLLLRAGTHPDFFELQPE
                     EADKPIRVDQVRELVDFVAQTAQLGGRKLVLLEPAEAMNLNAANALLKSLEEPAGDTV
                     LLLVSHQPSRLLPTIKSRCQQQACPLPGPAAALSWLAGRLPEQEGAVHQRLLELAAGS
                     PLRALQLYRDGVLEQRLLVEEGVKKLLKQQAAPSELAEGWKNVPLPLLLDWFCEWLLL
                     VLRFRMTGDEAGLGAPQMSKVMRYLAERAPLPAVLELQEWLLAQRQKVLGKANLNRVL
                     LLEALLVRWASLPLAG"
BASE COUNT          799 a         1645 c         1532 g          746 t
ORIGIN      
        1 gacgaactgt tctgcagccg caacttcggc ccggcgatcc acaagttcgg cgccatcctc
       61 ggcggcgcgt tcaacttcgt cgaccagaac ctgttcggcg gcaagatccc gctgaccctg
      121 cacgacaaca agccggacta cacctgcctg aagccggctg ccgagtcgca gaagatcgac
      181 taccccaagc cggacggcaa gctgagcttc gacaagctgt cctcggtgtt cctctccaac
      241 accaaccacg aggaagacca gccctgccac ctgaagctgg ccgatgcctc gatcccgctg
      301 agcaagaacc tgccgctgta cgacgaaccg gcgcagcgct actgcccggc cggagtgtac
      361 gagatcgtca cccaggaaaa cggcgagaag aagttccaga tcaacgcgca gaattgcgtg
      421 cactgcaaga cctgcgacat caaggatccg gcgcagaaca tcacctgggt cgctccggaa
      481 ggcaccggcg gaccgaacta ccccaacatg taacacgcgc gccagagagg ctccttcggg
      541 agcctttctg cattctggag cggacgccta ctccggctgc ggcgcgaccg ggaagaactc
      601 gccgccactc cacagcccca gccagagctg gccgtcgagc tgccgcggca ccagcagctc
      661 gaccagccgg tagaacacgt tgcggtggat cagtgcctcc aggttggcgc gcacatgcac
      721 gtagggcgaa ggctccccgg tctgcgcgtc cagcacgacc cgcagcgggt gctcgccccc
      781 ggccaccact tcgtcctcga cattggtgcg aaagcgcagc cgctgcgcct ctcccgcgcc
      841 ttccacctcg acctcgaccg ccacgaaggg cgcgtcgtcg acgcggatgc cgaccttctc
      901 caccggcgtg accagaaagt agtcgtcgcc atcgcgacgc aggatggtgg agaacagccg
      961 caccatcggc ttgcgcccga tcggcgtgcc catgtagaac cagctgccgt cgcgggcaat
     1021 gcgcatgtcg atatcgccac agaagggcgg attccacaga tgcaccgggg gcaggccctt
     1081 ccccggcgtc accggaattt gctgcagcag gtcgcccgcc ttgtccgatt cgttcattgt
     1141 cttcccctgt gaagtttccg ctggcgcgct tgcgcttatt atttacccag taaccgaata
     1201 cgtcattccg ctacgaacgg caatgacgcc cgagcgctgc cagcttgccg ccccagcccc
     1261 ccacccccgc attgcacgga gcccgacatg actgcatggc acgacgaatt cccccaggag
     1321 cccggcttgc gctacctgaa tcacgccgcc gtagccccct ggccacgccg agccagccag
     1381 gcggtgagcg cctttgccga gcagaacatg cgcgtcggcg cccgcgacta cccgcaatgg
     1441 ctgcagcttg aacagcgcct gcgcacgcgc ctgacccgcc tgctcaacgc cccgtccagc
     1501 gccgacgtgg cgctggtgaa gaacacctcg gaggcgctgt ccttcgtggc cttcggcctc
     1561 gactggcgca gcggcgacca ggtggtgatc agcgaccagg agttcccttc caaccgcgtg
     1621 gtgtgggagg ccctgcgccc gcagggggtg gaggtgatcg aggtgagtct cgccggcagc
     1681 gatcccgaga ccgccctgct cgccgcctgc accgcgcgca cccgcctgct ggcgatcagc
     1741 gcggtgcagt acgccagcgg cctgcgcctg gacatgacgc gcctcggcgc cggctgccag
     1801 cagcgcggcg tgctgctgtg catcgacgcc atccagcagc tgggtgccca gccgttcgac
     1861 gtgcaggcca gccagtgcgc cttcgccatg gccgacggtc acaagtggat gctcggcgcg
     1921 gagggtctcg gggtattcta ctgccgccgt gacctgcgcg agcagctgaa gctgcacgaa
     1981 tacggctggc acatgctgga ggatgtcggc aactacgacc ggcgcgactg gcagccggca
     2041 cagagcgcgc gacgcttcga gtgcggcagc ccgaacatgc tcggcgcggt ggccctggag
     2101 gccagcctgg cgctgctcga ggaagtcggc ctggcgcaag tggccagcct gatcgaggcg
     2161 cgcatcgccc ggctgcatgc ggggatcgcc gccctgcccg gcgccagcct gctgagtccg
     2221 ccggagccgg cgcgacgcgc cgggattctc accttcagcc tggccggctg ggagcacccg
     2281 cggctgttcg aggccctgaa acagcagcag gtggtctgtg cccagcgcgg cggcggcatc
     2341 cgcctgtcgc cgcacttcta tacgccggag caggtgatcg aggaaaccct ggaactgctg
     2401 cggcgactgc gctgaggtcg agcagagggg aggaatcccg caaaccgggc cgctttgcac
     2461 tcagcagccg cggaactatt tcaaatctgt tttaaaacag gggggatcct gccggcaaag
     2521 cgccggatgc tggtcaatac tgcttatcac tgatacggca cttcccccag tgtccatcag
     2581 gcggaacggt gaggaaggct tgttccggtt tcactcctaa tggtcttgac ccggattcac
     2641 ccccagaatc cgggtttttt ttgtctgcgc gttgctcaga ccgccagggt aaacaggcgc
     2701 tggaagttgg ccgtggtctg ctcggccagg gtctcgaagg ccacgccacg cagcccggcg
     2761 aggaactcgg cgacgtcgcg cacgtactcc ggcaggttcg gcttgccgcg gtgcggtacc
     2821 ggcgccagat agggcgcatc ggtttccacc agcaggcgat cggcggggat gcgccgcgcc
     2881 acctcgcgca gggcctcggc attgcggaag gtgacgatgc cggacaggga aatgtagtag
     2941 cccaggtcca gcgccgcctt ggccatgtcc cagtcctcgg tgaagcagtg cagtacgccg
     3001 gcctgcgcca ggtcggccgc gcgcagcatg tccagggtgt cctggcgcgc ttcgcgggta
     3061 tgcacgatca ccggcttgcc ggtctgctgc gccgcctcga gatgcaggcg gaagctggcc
     3121 tgttgcaacg tggccgcttc cggctggtag tggtagtcca gaccggtttc gccgatggcc
     3181 actacccgtg gatggtccag ctcgtgcagc aaccagtcga gtgccggctc cgcgccgggc
     3241 tcgagatcca gcgggtgcac accgatcgag caatacacgt cggcgtggct ttccgccagc
     3301 tcgcgcaccg ccgcggcgtt gtccgcgctg atgccgatgc acaggaagcg gccgacgccg
     3361 cgggcacgcg ctgccgccag cgcggcgtcg agcgaaccgc cgtgggccgc gaggtccagg
     3421 cgatcgagat ggcaatggga gtctacgagc atcgtcacat cgtcagggtc ggccgctcgg
     3481 acttgagcgc accggcgagg taggtttcga tcttgttgcg ggtggtgtgg tcgctgtcgt
     3541 tgagctgcac gccgaccccg gccacgcggt tgctctgggc gcccttgggg gtgacccaca
     3601 ccaccttgcc ggcgaccggg atcttttccg gctcgtccat caggctcagc agcatgaaga
     3661 cctcgtcgcc caggcgatac gtcttgttgg tggagatgaa caggccgccg ttgcgcacga
     3721 agggcatgta ggccgcatac aactgggcct tgtccttcag ggtaagagac aggatggcgt
     3781 tacgtccgcc aaggggaggc atgctcatgg gggtcttcca agactgatcc gatggtcgat
     3841 tctagcccgc cagcggcagg cttgcccacc gcaccagcag ggcttcgaga agcagtacgc
     3901 gattgaggtt ggccttgccg agcaccttct gccgctgcgc cagcagccac tcctgcagct
     3961 ccagcaccgc cggcagcggt gcgcgttcgg ccagatagcg catcaccttg ctcatctgcg
     4021 gcgctcccag cccggcctcg tcacccgtca tgcgaaaacg cagcaccagc agcagccact
     4081 cgcagaacca gtcgagcagc aggggcaggg gcacgttctt ccagccctcg gccagctcgg
     4141 aaggcgccgc ctgctgcttg agcagcttct tcacgccctc ctccaccagc aggcgttgct
     4201 ccagcacgcc gtcccgatac agctgcaggg cacgcagcgg cgacccggcc gccagctcga
     4261 gcaggcgctg atgcacggca ccctcctgct ccggcaggcg gccagccagc cagctcagcg
     4321 ccgccgccgg ccccggtagc gggcaggcct gctgctggca gcggctcttg atggtcggca
     4381 gcaaacggct gggctggtgg ctgaccagca gcagcacggt gtcgccggcc ggctcctcca
     4441 gacttttcag cagggcattg gcggcattca ggttcatcgc ctcggccggc tcgagcagga
     4501 ccagcttgcg cccgccgagc tgggcggtct gcgccacgaa gtcgaccagt tcacgcacct
     4561 ggtcgacgcg gatcggcttg tccgcctcct ccggctgcag ctcgaagaag tccggatggg
     4621 taccggcgcg cagcagcagg caggccttgc actgcccgca ggcgccggcg ccagtggcaa
     4681 gcggagcact gcacagcagc gccgcggcca gacgctcggc ca
//
LOCUS       DATCUN010000109         8841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_60712, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000109 DATCUN010000000
VERSION     DATCUN010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60712"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2462)
                     /gene="uvrA"
                     /locus_tag="VIO83_04215"
     CDS             complement(<1..2462)
                     /gene="uvrA"
                     /locus_tag="VIO83_04215"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY8330513.1"
                     /translation="MDKILIRGARTHNLKNIDLTLPRDKLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMEKPDVDTIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIYDYLRLLYARVGTPRCPDHDVPLEAQTVSQMVDLVLALPEGSKRMLLAPVIRERKG
                     EHLSVFEELRAQGFVRARVNGKLFELDELPKLDKQKKHSIDVVVDRFKVRDDLQQRLA
                     ESFETALKLADGIALVAPMDGEDGEEIIFSARFACPHCGHSISELEPKLFSFNNPAGA
                     CPSCDGLGVKQFFDPRRLVNGELTLAEGAIRGWDRRNVYYFQMLGSLAAHYDFSLEEP
                     FDDLPVAVQKALLHGSGREEVEFRYLNDRGDIVRRAHPFEGIVPNLERRYRETESTSV
                     REELAKLLSTQACPDCRGTRLRREARHVWVGDKTLPAVTALPVGDACGYFAELALTGR
                     RGEIAEKILKEIRERLQFLVNVGLDYLTLDRSADTLSGGEAQRIRLASQIGAGLVGVM
                     YILDEPSIGLHQRDNERLLATLTHLRNLGNTVIVVEHDEDAIRLADYVVDIGPGAGVH
                     GGRIVAEGTPAEVMAHPDSLTGKYLSGRERIRYPAERTPVDRDKLLRLKGARGNNLQN
                     VDLEIPVGLFTCITGVSGSGKSTLINNTLFPLAATALNGATSLEAAPHGSFDGLQHLD
                     KVVDIDQSPIGRTPRSNPATYTGLFTPIRELFAGVPESRSRGYNPGRFSFNVKGGRCE
                     ACQGDGVIKVEMHFLPDIYVPCDVCKGKRYNRESLEVKYKGKNIHEVLEMTIEEARAF
                     FDAVPALARKLQTLIDVGLSYI"
     gene            2591..3976
                     /locus_tag="VIO83_04220"
     CDS             2591..3976
                     /locus_tag="VIO83_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8330514.1"
                     /translation="MHDPHTERMSPTEQRAAAGLASVFAFRMLGMFMVLPVLATYGQD
                     LVGSTPLLIGLAIGAYGLTQALLQIPFGIVSDRVGRLPVIYVGLLIFAAGAALAATAD
                     SIWGVIAGRVLQGAGAISAAVMALLSDLTREQHRTKAMAMIGMSIGLSFAVAMVVGPL
                     LTRAFGLSGLFWVTAGMALLGILIIAVLVPKPQQALQHREARVASQALGATLRHPDLL
                     RLDFGIAALHAILMASFVALPLALVEQGGLPKEEHWWVYLVGLLVGFFGMIPFIIYGE
                     KKRQMKRVLLGAVALLVLCELFFWQFGSSLRALVIGNLVFFTAFNLLEASLPSLISKM
                     APAGGKGTAMGVYSTSQFLGAALGGLLGGWLFQQGGVSAVFLGCALLAALWLGFAATM
                     REPPYVTSVRLPLSPQAQREAGLAARLKATPGVADAFVVAEEAAVYVKVDTQQLDRTS
                     LERLVNPAPAC"
     gene            3993..4490
                     /locus_tag="VIO83_04225"
     CDS             3993..4490
                     /locus_tag="VIO83_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY8330515.1"
                     /translation="MARGVNKVILVGNVGGDPETRYLPNGNAVTNITLATSDSWKDKQ
                     TGQLQERTEWHRVVFFGKVAEIAGEYLRKGSQVYVEGRLQTREWEKDGVKRYTTEIVV
                     DMGGQMQLLGGRGGNAGDEAPRAPRAPREAQQQAPRQSRPAPQQPAAQQPAPDYDSFD
                     DDIPF"
     gene            4509..5393
                     /locus_tag="VIO83_04230"
     CDS             4509..5393
                     /locus_tag="VIO83_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEY8330516.1"
                     /translation="MRMRLMLLGGGNALGQALLRLGAEENIELQAPVPPAQGWDPASL
                     TCLLDEQRPDVLINLAYYHDWFQSEQVEVERLFVQERAIERLAELCLHHEIILLQPSS
                     YKVFDGARATAYSEKDDVRPLSLRGQALWRFEQLVRAVCPRHILLRFGWLLDESPEGR
                     LGRVLKWAESGEELFLADDRRGNPTPVDDAARVILSVLKQLDCNAPLWGTYHYGGIEA
                     TTSLAMGQATLAEARRYRPELPQDFTPQPHAERPDAPEEPQHAVLACKKILNTFGIKP
                     RAWRAGLPALLDRYYRHG"
     gene            5386..6315
                     /locus_tag="VIO83_04235"
     CDS             5386..6315
                     /locus_tag="VIO83_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016702656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8330517.1"
                     /translation="MAESRVLVTGGAGFIGSHLVDALLARGHAVRVLDNLSTGKRNNL
                     PLDNPRLELVVGDVADAAALRAALAGCTAVAHLAAVASVQASVDDPVSTHQSNFIGTL
                     NLCEAMREQGVRRVLFASSAAVYGQNGEGEAIDEDTPKAPLTPYAADKLASEYYLDFY
                     RRQHGLTPAIFRFFNVFGPRQDPSSPYSGVISIFAERALAGLPITVFGDGEQTRDFVY
                     VADLVGLLVQALELPQVEPGAVNVGLNRATSLNQLLVALGELLDGLPPVTYGAARPGD
                     IRHSRADNRRMLARFAFPEPTPIKTGLAGLLGL"
     gene            complement(6425..7102)
                     /locus_tag="VIO83_04240"
     CDS             complement(6425..7102)
                     /locus_tag="VIO83_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008081866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEY8330518.1"
                     /translation="MIRKTLSASLLALAIAAPFAQAFEAGDIIVRAGAITVDPQEDSS
                     DISTAVTGPLSGTKVGLDSDTQLGLNFAYMLTNNIGIELLAATPFTHDITAKGLPGGM
                     SGDFGEVSHLPPTLSVVYYPLASQSAFQPYVGLGVNYTWFFDDSLSSKAESDGFNGLE
                     LDDSWGLAAQVGMDYMLTDNVMLNAQVRYIDIDTEATSNHAALGKVKVDVDVDPMVYM
                     VGLGYKF"
     gene            complement(7283..8152)
                     /gene="trxA"
                     /locus_tag="VIO83_04245"
     CDS             complement(7283..8152)
                     /gene="trxA"
                     /locus_tag="VIO83_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY8330519.1"
                     /translation="MSESPYIFDVSTTSFEQLVIENSFHKPVLVDFWAEWCAPCKALM
                     PLLAKIAEGYQGELLLAKVNCDIEQDIVMRFGIRSLPTVVLFKDGQPVDGFAGAQPES
                     AIRTMLEPHVPEPAPAPADPLESARAQYAAGQIGAAEQLLKQLLGEDNQNAAALILYA
                     RCLAERGELDEAQAVLDAVKDDAHKQELAAARAQLTFLRRVVGLPEAADLKSRLAQDA
                     GDDEAAFQLSIHLLARQQYEAALDGLLRLFIRNRSYGDDLPRKTLVQVFDLLGNEHPL
                     VVAYRRKLYQALY"
     gene            8375..>8841
                     /locus_tag="VIO83_04250"
     CDS             8375..>8841
                     /locus_tag="VIO83_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEY8330520.1"
                     /translation="MSAVADFRQRYLVRFWSPLPALVALGIASAYYFALTGTFWAVTG
                     EFTRWGGHILSWFGYQPQEWSYFKLIGLQGTPLERIDGVMIVGMFVGALCTALWAGNV
                     GLRWPTSKRRLLQGLLGGIVAGFGARLAMGCNLAAFFTGIPMFSLHAWAFMLAT"
BASE COUNT         1336 a         2848 c         3079 g         1578 t
ORIGIN      
        1 ttgatgtacg agaggccgac gtcgatcagc gtctgcagct tgcgcgccag cgccggtacc
       61 gcatcgaaga aggcgcgggc ctcctcgatg gtcatttcca gtacctcgtg gatgttcttg
      121 cccttgtact tcacctcgag gctttcgcgg ttgtagcgct tgcccttgca cacgtcgcag
      181 ggcacgtaga tgtccggcag gaagtgcatc tccactttga tcacgccgtc gccctggcag
      241 gcctcgcagc gcccgccctt gacgttgaag gagaagcgcc ccgggttgta gccgcgcgag
      301 cgcgattccg gcacgccggc gaacagctcg cggatcggcg tgaacagtcc ggtgtaggtg
      361 gccgggttgg agcgcggcgt gcgcccgatc gggctctggt cgatgtcgac caccttgtcg
      421 agatgctgca ggccgtcgaa ggagccatgc ggcgccgcct ccagcgaggt ggcgccgttc
      481 agtgcggtgg cggccagcgg gaacagggtg ttgttgatca gcgtcgattt gccggagccg
      541 gacaccccgg tgatgcaggt gaacaggccg accgggattt ccaggtcgac gttctgcagg
      601 ttgttgccgc gcgcgccctt gagccggagc agcttgtcgc ggtcgacggg ggtgcgttct
      661 gccgggtagc ggatcctctc gcgtcccgac aggtacttgc cggtcagcga gtcgggatgg
      721 gccatcacct cggccggagt gccctcggcg acgatgcgcc cgccgtgcac gccggcgccg
      781 gggccgatgt cgaccacgta gtcggccagg cggatggcgt cctcgtcgtg ctcaaccacg
      841 atcaccgtgt tgccgaggtt gcgcaggtgg gtgagggtgg ccagcaggcg ctcgttgtcg
      901 cgctggtgca ggccgatcga cggctcgtcg aggatgtaca tgacgccgac caggccggcg
      961 ccgatctggc tggccaggcg gatgcgctgt gcctcgccgc cggacagggt gtcggcgctg
     1021 cgatccagag tcaggtagtc gaggccgacg ttgaccagaa actgcaggcg ctcgcggatc
     1081 tccttgagga tcttctcggc gatctcgccg cggcgaccag ttaacgccag ctcggcaaaa
     1141 tagccgcagg catcgcccac cggcagggcg gtgaccgccg gcagggtctt gtcgccgacc
     1201 cacacgtggc gcgcctcgcg gcgcaggcgg gtgccgcggc agtccgggca ggcctgggtg
     1261 ctgagcagct tggccagctc ctcgcgcacg ctggtggatt cggtctcccg gtagcggcgc
     1321 tcgaggttgg gcacgatgcc ctcgaacggg tgggcgcggc ggacgatgtc gccgcggtcg
     1381 ttgaggtagc ggaactccac ctcctcgcgg ccgctgccgt gcagcagggc cttctgcacg
     1441 gccaccggca ggtcgtcgaa cggctcttcc aggctgaagt cgtagtgcgc ggccagtgag
     1501 ccgagcatct ggaagtagta gacgttgcgt cggtcccagc cgcggatcgc cccctcggcc
     1561 agggtcagct cgccgttgac cagtcggcgc gggtcgaaga actgcttcac ccccaggccg
     1621 tcgcagctcg ggcaggcgcc cgccgggttg ttgaaggaga acagcttggg ttccagctcg
     1681 ctgatcgagt ggccgcagtg cgggcaggcg aagcgcgcgg agaagatgat ttcctcgccg
     1741 tcctcgccat ccatgggggc gaccagggca atgccgtcgg ccagcttgag ggcggtctcg
     1801 aaggactcgg ccaggcgctg ctgcaggtcg tcgcgcacct tgaagcgatc caccaccacg
     1861 tcgatgctgt gcttcttctg cttgtcgagc ttgggcagct cgtccagctc aaaaagcttg
     1921 ccgttgaccc gggcgcggac gaagccctgg gcgcgcagct cctcgaacac cgacaagtgt
     1981 tcgcccttgc gctcgcggat caccggtgcc agcagcatgc gcttgctgcc ttcgggcagg
     2041 gctagcacca ggtcgaccat ctggctgacc gtctgcgcct ccagcggcac gtcgtggtcc
     2101 gggcagcgtg gggtgccgac gcgggcgtag agcaggcgca ggtagtcgta gatctcggtg
     2161 atggtgccga ccgtggagcg cgggttgtgc gaggtggact tctgctcgat ggagatcgct
     2221 ggcgacaggc cctcgatggt gtcgacgtcg ggcttctcca tcatcgacag gaactggcgg
     2281 gcgtaggcgg agagggattc cacgtagcgg cgctggccct cggcgtacag ggtgtcgaag
     2341 gccagcgacg acttgcccga gccggacagg ccggtgatca cgatcaactt gtcgcgcggc
     2401 agggtcaggt cgatgttctt caggttgtgg gtacgtgccc cacgaatcag gatcttgtcc
     2461 actgcggcct cgcatggcgg gcggaaaccc gcgagtatac ggccgccttc ggccgagcgg
     2521 caaagcgccg cgcctgtgcc ggcgcacgcg gtgctgctag aatcgtgccc ccgtttttcg
     2581 aggtgttgtc atgcacgatc cgcacactga gcgcatgagt ccgaccgagc agcgcgcggc
     2641 tgccggcctg gcctcggtgt tcgctttccg catgctcggc atgttcatgg ttttgccggt
     2701 gctggcgacc tacggccagg acctggtggg ctccacgccg ctgctgatcg gcctggccat
     2761 cggcgcctac ggcctgaccc aggcgctgct gcagatcccc ttcggcatcg tttccgaccg
     2821 cgtcggccgc ctgccggtga tctacgtcgg cctactgatc ttcgctgccg gcgcggcgct
     2881 ggccgccact gccgactcca tctggggggt gatcgccggg cgcgtgctgc agggcgccgg
     2941 agcgatctcg gcggcggtga tggcgctgct ctccgacctg acccgcgaac agcaccgcac
     3001 caaggcgatg gcgatgatcg gcatgagcat cggcctgtcg ttcgccgtgg ccatggtggt
     3061 cggtccgctg ctgacccgcg ccttcggcct gtccgggctg ttctgggtca ccgccggtat
     3121 ggccctgctc ggcatcctga tcatcgccgt gctggtgccc aagccgcagc aggccctgca
     3181 gcatcgcgaa gcgcgcgtgg cgagccaggc gctgggcgcg acgctgcgcc atcccgacct
     3241 gctgcgcctg gacttcggta tcgccgcgct gcacgccatc ctcatggcca gctttgtcgc
     3301 cctgccgctg gcgctggtgg agcagggcgg gctgcccaag gaggagcact ggtgggtgta
     3361 cctggtcggc ctgctggtcg gcttcttcgg catgatcccg ttcatcatct acggcgagaa
     3421 gaagcgccag atgaagcgcg tgctgctcgg tgcggtggca ctgctggtgc tgtgcgagct
     3481 gttcttctgg cagttcggca gcagcctgcg cgcgctggtg atcggcaatc tggtgttctt
     3541 caccgccttc aacctgctgg aagcctcgct gccgtcgctg atcagcaaga tggcgccggc
     3601 cggcggcaag ggcacggcga tgggcgtgta ctccaccagc cagttcctcg gcgccgccct
     3661 cggcggcctg ctcggtggct ggctgttcca gcagggcggg gtgagcgccg tgttcctcgg
     3721 ctgtgcgctg ctggctgccc tgtggctggg gtttgctgct actatgcgcg aaccgccgta
     3781 tgtgaccagt gtgcgcctgc cgctgtctcc ccaggcccag cgcgaggcgg gactggccgc
     3841 gcgcctgaag gcaacgcccg gcgtggccga tgccttcgtg gtggccgaag aggccgccgt
     3901 ctacgtcaag gtggataccc aacaactgga tcgcacgtcg ctcgaacgcc tggtcaatcc
     3961 ggcgccggcg tgctgaagcc gaggagaatt ctatggctcg cggagtcaac aaagtcattc
     4021 tggtgggtaa cgtcggtggc gacccggaaa cccgttacct gcccaacggc aatgcggtga
     4081 ccaacatcac cctggccacc agcgacagct ggaaggacaa gcagaccggc cagctgcagg
     4141 agcgcaccga gtggcaccgc gtggtgttct tcggcaaggt cgccgagatc gccggcgagt
     4201 acctgcgcaa gggctcccag gtgtacgtcg aaggccgcct gcagacccgc gagtgggaaa
     4261 aggacggcgt caagcgctac accaccgaga tcgtggtcga catgggtggc cagatgcagc
     4321 tgctcggcgg gcgtggcggc aatgccggcg acgaagcgcc gcgcgccccg cgtgctccgc
     4381 gcgaagcgca gcagcaggcg ccgcgccaat cgcgtcctgc tccgcagcaa ccggccgccc
     4441 agcagccggc gccggactac gacagcttcg acgacgacat cccgttctaa ccgaggtagt
     4501 ccatcaccat gcgcatgcgc ctgatgctgc tgggcggcgg taatgccctt ggacaggcgc
     4561 tgctgcgctt gggggcggaa gagaacattg agcttcaggc cccggtgccg cccgcacagg
     4621 gctgggatcc cgccagcctg acctgcctgc tggacgagca gcgccccgac gtgctgatca
     4681 acctggccta ctaccatgac tggttccagt ccgagcaggt cgaggtcgag cggctgttcg
     4741 tccaggagcg cgccatcgag cggctggccg agctctgcct gcaccacgag atcatcctgc
     4801 tgcagccttc cagctacaag gtgttcgacg gcgcccgcgc cacggcctac agcgagaagg
     4861 acgacgtgcg cccgctgagc ctgcgtggcc aggcgctgtg gcgcttcgag cagctggtgc
     4921 gggccgtctg cccgcggcat atcctgctgc gcttcggctg gctgctggac gagagtccgg
     4981 aggggcgcct ggggcgggtg ctcaagtggg ccgagagcgg cgaggagctg ttcctcgccg
     5041 acgaccgccg cggcaatccg accccggtgg acgacgccgc gcgggtgatc ctctcggtgc
     5101 tcaagcagct cgactgcaac gcgcccctgt ggggcaccta tcattacggt ggcatcgagg
     5161 cgaccaccag tctggccatg gggcaggcga ccctggccga ggcccgccgc taccggccgg
     5221 agctgcccca ggacttcacg ccgcaacccc atgccgaacg gccggacgcc ccggaagagc
     5281 cgcagcatgc ggtgctcgcc tgcaagaaaa tcctcaacac cttcggcatc aagccgcgcg
     5341 cctggcgtgc cggcctgccg gcattactgg atcgctacta ccgtcatggc tgagtcccgc
     5401 gtcctggtca ccggcggcgc cggcttcatc ggctcgcacc tggtcgacgc tctgctggcg
     5461 cgtggtcacg ccgtgcgcgt gctggacaac ctgtccaccg gcaagcgcaa caacctgccg
     5521 ctggataatc cgcgcctcga actggtggtg ggcgatgtcg ccgatgcggc ggcactgcgc
     5581 gcagcactcg ccggctgcac ggcggtggcc catctggccg cggttgcttc ggtgcaggcc
     5641 tcggtggacg atccggtgag tacccaccag agcaacttca tcggcacgct gaatctgtgc
     5701 gaggcgatgc gcgaacaggg cgtacgtcgg gtgctgttcg cctccagtgc ggcggtgtac
     5761 gggcagaacg gcgagggcga ggcgatcgac gaggacacgc cgaaggcgcc gttgacgccc
     5821 tacgcggcgg acaagctggc cagcgagtac taccttgatt tctaccgtcg ccagcatggc
     5881 ctcacgcctg cgatcttccg cttcttcaac gtcttcggcc cgcgccagga tccgtcttcg
     5941 ccgtactcgg gggtgatcag catcttcgcc gagcgggctc tggccggcct gccgatcacc
     6001 gtgtttggtg acggcgagca gacccgcgac tttgtctacg tggccgatct tgtagggctg
     6061 ctggtgcagg cgttggagct gccgcaggtg gagccggggg cggtgaacgt tggcctgaac
     6121 cgcgccacca gtctcaacca gttgctggtg gctctcggcg agctgctcga cggcctgccg
     6181 ccggtgacct atggcgccgc gcggcccggc gatatccgtc actcgcgggc ggacaaccgg
     6241 cgaatgctgg cgcgcttcgc cttccccgag ccgacgccga tcaagaccgg gctggcgggg
     6301 ttgctcggtc tctgaggttt gccagatgcc actgggtggc gtccgtcagc ggtgagccgc
     6361 tacaaatgca aaaggcaccc gaaggtgcct tttgtcgttt ctgacgttgc cggggtagcg
     6421 gttatcagaa cttgtagccg aggccgacca tgtagaccat cggatcgacg tcgacgtcga
     6481 ctttgacctt gccgagggcg gcatggttgg aagttgcttc agtgtcgatg tcgatgtaac
     6541 ggacttgggc gttgagcatc acgttgtcgg tcagcatgta gtccatgccc acctgtgccg
     6601 ccaagcccca ggaatcatcc agctccaagc cattgaagcc atcactctca gctttgctgc
     6661 tcaggctgtc atcgaagaac caggtgtagt tgacgccgag gccgacatac ggctggaagg
     6721 cagactggct ggccagcggg taataaacca cactcagggt cggcggcagg tggctgactt
     6781 caccgaagtc gccggacatg ccacctggca ggcctttggc ggtaatgtcg tgagtgaagg
     6841 gggtggctgc cagcaactca atgccgatgt tgttggtcag catgtaggcg aagttcaggc
     6901 ccagctgggt atcgctgtcc agacctacct tggtgccact gagcgggcca gtcacggcag
     6961 tggagatatc gctgctgtct tcctgcggat caacggtgat cgcgccggca cgaacgatga
     7021 tgtcgccggc ttcgaaggcc tgggcgaagg gagcggcgat ggccaaggcc agcagggagg
     7081 cgctgagggt tttgcggatc atggtgagct ccaataacaa taagttctta gcgatctgct
     7141 gcctatgcta acgactggag tgctcattgt cttgacccag ctcaacagat aagtgcagcc
     7201 gagcgccagc gggctggcgc agacgtggct gggccacgcc ggttcgctgc tccacagcaa
     7261 cggcgcgagg ccgacggcgg actcagtaca gtgcctgata cagcttgcgt cggtaggcaa
     7321 ccaccagcgg atgctcgttg ccgagcaggt cgaacacctg caccagggtc ttgcgtggca
     7381 ggtcgtcgcc gtagctgcgg ttgcggatga acagacgcag caggccatcc agtgccgcct
     7441 cgtactgctg gcgggcgagc aggtggatgc tcagctggaa ggcggcctcg tcgtcgccgg
     7501 cgtcctgggc caggcggctc ttcaggtcgg cagcttccgg caggccgacc acccggcgca
     7561 ggaaggtcag ctgggcgcgc gcggcggcca gttcctgctt gtgcgcgtcg tccttcaccg
     7621 catccagaac cgcctgcgcc tcgtccagct cgccgcgctc ggccaggcag cgggcgtaga
     7681 ggatcagcgc cgcggcattc tggttgtcct cgccgagcag ctgcttgagc agttgttccg
     7741 ccgcgccgat ctgcccggcg gcgtactgcg cgcgcgcact ttccagggga tcggccggtg
     7801 ccggagccgg ctccggcaca tggggttcga gcatggtgcg gatcgccgac tccggctggg
     7861 caccggcaaa gccgtcgacc ggctggccgt ccttgaacag caccacggtg ggcaggctgc
     7921 ggataccgaa gcgcatgacg atgtcctgct cgatatcgca gttgaccttg gccagcagca
     7981 gctcgccctg gtaaccctcg gcgatcttcg ccagcaacgg catcagcgcc ttgcatggcg
     8041 cgcaccactc ggcccagaag tccaccagca ccggcttgtg gaaggagttt tcgatcacca
     8101 gttgttcgaa gctggtggtg gagacgtcga agatgtacgg gctttcgctc atcggggttc
     8161 tcgagacggg cgcaggatgg cgtgcgcagg aaaaatgctg tgcatggtaa gtgggggcgc
     8221 tcgcctggag aagcaagggt tggccgtgcc ggtgcgcagg caaagttgcc gtgcgggacg
     8281 tggtggcgct gacgtgcccg cggcgccagg cgtagaatcc gcgccttttg gcgttcgggc
     8341 gtcgcgcggt ggtgcccggc aaggagagtg ttgcatgtct gcagtagccg atttccgaca
     8401 acgttacctg gtgcgtttct ggtcgccctt gccggcgctg gtcgcactgg ggatcgcttc
     8461 cgcgtactac ttcgccctca ccgggacctt ctgggcggtg accggcgagt tcacccgctg
     8521 gggtggacac atcctctcct ggttcggcta ccagccgcag gagtggagct acttcaagct
     8581 gatcggcctg cagggcacgc cactggagcg catcgacggg gtgatgatcg tcggcatgtt
     8641 cgtcggcgcc ctgtgcacgg cgctgtgggc cggcaatgtc ggcctgcgct ggccgaccag
     8701 caagcgccgc ctgctgcagg ggctgctcgg cggcatcgtc gccggcttcg gcgcgcgtct
     8761 ggccatgggc tgcaacctgg ccgccttctt caccggaatt ccgatgttct ccctgcatgc
     8821 ctgggccttc atgctcgcca c
//
LOCUS       DATCUN010000110         9651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_155042, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000110 DATCUN010000000
VERSION     DATCUN010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9651
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155042"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4247)
                     /locus_tag="VIO83_04255"
     CDS             complement(<1..4247)
                     /locus_tag="VIO83_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY8330521.1"
                     /translation="MGDRHDYVALDWVKGEIAETLKQARQALEAFVESSQDLTRLRFC
                     LSYVHQVQGTLQMVEFYGAALLAEEMEQLLQALIDGRASTQGDALPVLMQAILQLPAY
                     LDRIQSARRDLPLVVLPLLNDLRNARGEKLLSETSLFAPDLSARVAALPASTLERLEA
                     AGLPTLLRKLRQMLQVALVGFMRNQEPATQLAYMAKVFAKLESLCKGAPIGALWPVAA
                     GVVEGLSNGSVASGSTVCTLLREVDHELKRLIDEGVGALNQPAPDELLKNLLFYIAKS
                     PSSEGRIAALKEHYRLAEAMPDSQVVDEERARMAGPDRDAMRSVVGALCEELVRIKDS
                     LDLFVRSDRRQVTDLAALQGPLKQVADTLAVLGFGQQRKVVADQLAMVALLIGGQQQP
                     SDAVLMDIAGALLFVEATLSGMVGPAEGERAEENLLPTTDVGQIHQLVIKEARTGLEQ
                     SKDAIIEFIASQWNHEHLARVPELLTQVRGGLGMIPLPRVAALLDACCRYIREQLLVR
                     QAVPSWQSLDTLADAITSVEYYLERISEDHASQGELILDVAEESLSSLGYGLQPTRSI
                     LDMPSEVLITAEPHFALVEPAVAVHQAAVVEAAAQATPAAVPPAAAAEPANAGEVASE
                     AIAEPALAAEAVSDAIAEPTPMPIDATEQPALESVAPAAEVEALAAPTLAQVMAAPVV
                     AINPPARDVPPQLLPPPADEEPMDEDLQEVFIEEAGEVLDTLNEYLPQWSADTANREA
                     LTEVRRAFHTLKGSGRMVRALVIGELAWSIENMLNRVIDRTIQPDAPLQQVIRAVVDL
                     LPALVEEYAAKGQRQRDDVDLLAAAAHALSKGQPLPTAGVAAAVVAPAGETAVAAEAS
                     SPVVVETAPVVAPVADDSAALLDPQLLEIFRAEAEAHLEALDRYLAACERELPQPVTD
                     ELQRALHTLKGSAYMAGIQPIAEVAAPLEKLAKEFKTNLIPFGLVAAELLRDAAKILR
                     HGLEQLATQPLSSLPGSTPLLQRVQQLQQEQLENAEAEWRSREGDSRDPQLISQFLAE
                     GMDILLDADELLVRWRQHPAEHEELDALRNELATLAQGAQQAELPQVDLLCAALLAIY
                     VAVGESRLAVDEAFFREVEAAHEALIGMMDQVAAGLEVTPQPERVQALQQLLDHAAAA
                     AAAALEVPPVDLLPESSSFEHPAEAEAEAEAEAEAELESESESESESESEPESQVESP
                     AAPAPLGEAVTEFELAPGLLAEPEWPAALVELPAVEMAPLTAEEDAPDPEMVEIFLEE
                     AGDILDSAAGALERWLVEPQARSALAALQRDLHTLKGGARMAGVRAIGDLAHELESIY
                     EGLSDGRFAMAAAFARLLHEGHDLLARQVEELQQGLPLTAAGSLLEQMRNLRQGVVPQ
                     LPASLDEAHPEAEAEAEAEAEAEAEAEAEAEAEAEAEA"
     gene            complement(4272..5135)
                     /locus_tag="VIO83_04260"
     CDS             complement(4272..5135)
                     /locus_tag="VIO83_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEY8330522.1"
                     /translation="MQPSAVWAMKPLPDITAAEFRAWQELLETRSGMVLNEQRRAFLQ
                     TILGTRLRELGLDSYASYFQQVTDGPRGAVEWSNLMDHLTVQETHFFRHPPSFQHLAV
                     YLRERLGVVQEGRAWQLWSVGCASGEEAYSLALVAAEVQREAGLSGRFGVTGTDISLN
                     ALARARSGRYGARRVGEVGGELLERYFLEQPDGGFQVCEALRERTCFVRLNVLDLARA
                     PQRGMDVIFCQNLLIYFRRWRRRDILARLVERLAPGGLLVIGVGEVADWSHPLLLPVA
                     DEQVLAFTRKG"
     gene            complement(5198..7246)
                     /locus_tag="VIO83_04265"
     CDS             complement(5198..7246)
                     /locus_tag="VIO83_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8330523.1"
                     /translation="MKKLNAGNLFSGTRSSSLIGGLFAILIVSVVLLFANFAYLNQQS
                     EYDKQYIAHAGELRLLSQGIAKNAVEAAAGKADAFALLKSERDDFQQRWEWLSQGDAD
                     TGLPASPEAVASNLATVQADWERLRTSTDAILASQQTVLSLHQVASTLAETIPQLQVE
                     YEEVVDILLENGAPADQVAVAQRQSLLAERILAAVNKVLAGAEDSVQAADSFGRDASL
                     FGRVLTGMHEGNPAMSITKVTDAEAMTRLKEITELFSFVSGSVDEILETSPELFQVRE
                     SANNIFGESQKVLEGSTQLASGFENLAVSRTFNTLAGYLLGLLALASIMLMALILVRE
                     TRDRLVETAEKNDRNQSAILRLLDEIGDLADGDLTVQATVTEDFTGAIADSINYSIDQ
                     LRDLVETINQTAVQVAAAAQETQATAMHLAEASEHQAQEIAGASAAINEMAVSIDQVS
                     ANAAESSAVAERSVAIANKGNEVVQNTIAGMDNIREQIQDTSKRIKRLGESSQEIGDI
                     VSLINDIADQTNILALNAAIQASMAGDAGRGFAVVADEVQRLAERSSAATKQIEALVK
                     TIQTDTNEAVISMEQTTTEVVRGARLAQDAGVALEEIEKVSRSLAALIQNISNAARQQ
                     SSSAGHISNTMYVIQEITTQTSSGTTATARSIGTLAKMATDMRKSVSGFTLPEHREQA
                     "
     gene            complement(7330..7872)
                     /locus_tag="VIO83_04270"
     CDS             complement(7330..7872)
                     /locus_tag="VIO83_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY8330524.1"
                     /translation="MSDVMTPFQHLIEIDQLCRRYSAGLPAQQEAAQSWSGIGFRMGT
                     RLFVAPMGEVTEVLHEPRYTLLPGVRSWVKGVANVRGRLLPIMDLCGFFGSELSPLRK
                     QRRVLVVEHGEVFAGLIVDEVFGMQHFSVEAFAEQLPPLEASIQPFIHGVFQRERPWL
                     VFSPHALARAQAFLDVAVNN"
     gene            complement(7922..8287)
                     /gene="pilH"
                     /locus_tag="VIO83_04275"
     CDS             complement(7922..8287)
                     /gene="pilH"
                     /locus_tag="VIO83_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilH"
                     /protein_id="HEY8330525.1"
                     /translation="MARILIVDDSPTEMYKLTAMLEKHGHQVLKAENGADGVALARQE
                     KPDVVLMDVVMPGLNGFQATRQLTKDAETGHIPVIIVTTKDQETDRVWGKRQGARDYL
                     TKPVDEETLLKTIAAVQAG"
     gene            complement(8334..8744)
                     /gene="pilG"
                     /locus_tag="VIO83_04280"
     CDS             complement(8334..8744)
                     /gene="pilG"
                     /locus_tag="VIO83_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HEY8330526.1"
                     /translation="MEQHSESLKVMVIDDSKTIRRTAETLLKKVGCDVITAVDGFDAL
                     AKIADSHPGIIFVDIMMPRLDGYQTCALIKNNSAFKSTPVIMLSSKDGLFDKAKGRIV
                     GSDQYLTKPFSKEELLGAIKTHVPGFVPVEQATT"
     gene            8991..>9651
                     /gene="gshB"
                     /locus_tag="VIO83_04285"
     CDS             8991..>9651
                     /gene="gshB"
                     /locus_tag="VIO83_04285"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY8330527.1"
                     /translation="MSVRLGIVMDPIARIAFKKDSSLAMLFAAQARGWSLFYMEQQDL
                     YLRDGQVRARTRPLQVIHDAKHWFHFDDEQDLALHELDVVLMRKDPPFDNEFLYCTQL
                     LEQAEREGLLVVNRPQSLRDCNEKLFATQFPQCTPPTLVSRRVDILREFAAEQRDIIL
                     KPLDGMGGSSIFHHRAGDPNLSVILETLTGHGSRQIMAQRYLPAIKDGDKRILMIDGE
                     PI"
BASE COUNT         1604 a         3206 c         3129 g         1712 t
ORIGIN      
        1 gcttcggctt cggcttcggc ttcggcttcg gcttcggctt cggcttcggc ttcggcttcg
       61 gcttcggctt cggcttcggc ttcaggatgt gcttcgtcga ggctcgccgg caactgcggc
      121 acgacgccct ggcgcagatt gcgcatctgt tcgagcaagc tgcccgcggc ggtcagcggc
      181 agcccctgct gcaactcctc cacctgacgg gccagcaggt cgtggccctc gtgcagcagg
      241 cgggcaaagg cggcagccat ggcaaagcgg ccatcgctca gcccctcgta gatcgactcc
      301 agctcgtggg ccagatcgcc aatggcgcgc acgccggcca tccgtgcccc acccttgagg
      361 gtgtgcaggt cgcgctgcag ggctgccagc gccgagcgag cttgcggctc gaccagccag
      421 cgctcgagcg cgccggcagc gctgtcgaga atgtcgccag cctcctcaag gaagatctcg
      481 accatttccg gatcgggggc gtcttcctcg gcagtcaatg gcgccatttc caccgccggc
      541 agctcgacga gcgccgcggg ccattcgggc tcggccaaca ggccgggcgc cagttcgaac
      601 tccgtgacgg cctcacccag cggagccggc gcggccgggg actcaacctg ggattcgggt
      661 tcggattcgg attcggattc ggattcggat tcggattcga gttcagcctc agcctcagcc
      721 tcagcctcag cctcagcctc agccggatgc tcgaaggacg acgactccgg cagcaagtcg
      781 actggcggga cttccagcgc agcggcagcg gcagcggcag catggtccag cagctgctgc
      841 agcgcctgca cgcgctccgg ttgcggcgtg acctcgaggc cggccgccac ctggtccatc
      901 atgccgatca gcgcctcgtg ggccgcctcg acctcgcgga agaaggcctc atccaccgcc
      961 agacgcgact cgccgacggc gacgtagatg gccagcaagg ccgcgcagag caggtcgacc
     1021 tgcggcagct cggcctgctg ggccccctgg gccagggtgg ccagctcgtt gcgcagggca
     1081 tccagctcct cgtgctcggc cggatgctgg cgccagcgga cgagcagctc gtcggcatcc
     1141 agcaggatat ccatgccctc ggcgaggaac tggctgatca gctggggatc gcggctgtcg
     1201 ccttcgcgac tgcgccactc ggcctcggca ttctccagtt gctcctgctg cagctgctgg
     1261 acccgctgca gcagcggggt gctgcctggc aggctcgaca gcggttgcgt agcgagctgc
     1321 tcgagaccat ggcgcaggat cttcgccgca tcgcgcagca gttcggcagc caccagaccg
     1381 aaggggatca ggttggtctt gaactccttg gccagcttct ccaagggggc cgccacctcg
     1441 gcaatcggct ggatgccggc catgtaggca ctgcccttga gggtatgcag ggcgcgctgc
     1501 aactcgtcgg tgaccggttg cggcagctcg cgctcgcagg cggccagata gcggtcgagc
     1561 gcctcgagat gagcctcggc ctcggcgcgg aagatctcca gcagttgcgg gtcgagcaga
     1621 gcggcgctgt cgtcggcaac cggcgccacc actggggcag tctcgaccac gaccggcgac
     1681 gacgcctcgg cagcaaccgc ggtttcgcct gcaggcgcaa caaccgctgc ggccacgccg
     1741 gccgtcggca gcggctggcc cttggacagc gcatgggcag cggcagccag caggtcgaca
     1801 tcgtcgcgct ggcgctggcc cttggcggca tactcctcga ccagggccgg cagcaggtcg
     1861 acgaccgcgc ggatcacctg ctgcagcggc gcgtcgggct ggatggtgcg gtcgatgacc
     1921 cggttgagca tgttctcgat cgaccaggcc agctcgccga tgaccagggc gcggaccatg
     1981 cgcccgctgc ccttgagggt gtggaaggcg cgccgcactt cggtgagcgc ctcgcgatta
     2041 gcggtgtcgg cagaccactg cggcaggtat tcgttgaggg tgtcgagaac ctcgccagcc
     2101 tcctcgatga acacttcctg caggtcctcg tccatcggct cttcgtcggc cggcggcggc
     2161 agcagctggg gcggcacgtc cctggcgggc ggattgatgg caacgaccgg cgcagccatc
     2221 acctgggcca gcgtcggcgc ggcaagcgcc tcgacctcgg ccgccggcgc gacggattcg
     2281 agcgctggct gctccgtcgc gtcgatgggc atgggcgtcg gctcggcgat ggcgtcagac
     2341 accgcttcgg cagcaagcgc tggctcggcg atggcctcag acgccacttc gccagcattc
     2401 gctggctcgg ccgcggcagc cggggggaca gcggccggcg tagcctgtgc ggcggcctcg
     2461 accacggcgg cttggtgaac ggcgacagcc ggctcgacca gcgcgaaatg cggctcggcc
     2521 gtgatcagaa cctcgctggg catgtcgaga atcgagcggg tcggctgcag tccgtagccc
     2581 agcgaactga ggctttcctc ggcgacgtcg aggatcagtt cgccctggct ggcatggtct
     2641 tcggaaatcc gctcgaggta gtactcgacg ctggtgatcg cgtcggccag ggtatccaga
     2701 ctctgccagc tgggcacggc ctggcggacc agcagctgct cgcggatgta gcggcagcag
     2761 gcatccagca gcgcggcgac ccgcggcaac ggaatcatgc cgagaccgcc acgcacctgg
     2821 gtcagcagct cggggacgcg ggccaggtgc tcgtgattcc actgcgaggc gatgaattcg
     2881 ataatcgcgt ccttggactg ctcgagcccg gtacgcgctt ccttgatgac cagctggtga
     2941 atctggccga cgtcggtggt cggcagcagg ttctcctcgg cacgctcccc ctccgccggt
     3001 ccgaccatcc cggacagggt ggcctcgacg aacagcaggg cgccggcgat atccatcagc
     3061 accgcgtcac tgggctgctg ctggccgcca atcagcaggg caaccatcgc cagctgatcg
     3121 gccaccacct tgcgctgctg gccgaaaccg agcacggcca gggtgtcggc gacctgcttg
     3181 agcggcccct gcagggccgc cagatcagtc acctgacggc ggtcgctgcg cacgaacaga
     3241 tcgaggctgt ccttgatccg caccagctcc tcgcacaggg cgccgaccac cgaacgcatg
     3301 gcatcgcggt cgggaccggc catgcgcgcc cgctcctcgt ccacgacctg gctgtcgggc
     3361 atcgcctcgg ccagtcgata gtgttccttc agcgcggcga tacggccctc gctgctcgga
     3421 ctcttggcga tgtagaacag cagattcttc agcagctcgt cgggcgcagg ctggttgagc
     3481 gcaccgacac cctcgtcgat caggcgcttg agctcgtggt cgacctcgcg cagcagggtg
     3541 cacacggtgc tgccggaggc aacgctgccg ttactcaggc cctcgaccac gccggcggcc
     3601 accggccaca gggcaccgat aggcgccccc ttgcacaggc tttccagctt ggcgaacact
     3661 ttggccatgt aggccagctg ggtcgccggc tcctggttgc gcatgaagcc gaccagcgcc
     3721 acctgcagca tctgccgcag cttgcgcagc agcgtcggca ggccggcggc ctccaggcgt
     3781 tcgagggtgc tcgccggcag cgcggctacc cgggccgaaa ggtccggagc gaacaggctg
     3841 gtttccgaca gcagtttctc gccgcgcgca ttgcgcagat cgttgagcag cggcaggacc
     3901 accaacggca ggtcgcggcg ggcgctctgg atgcggtcga ggtaggccgg cagttgcagg
     3961 atcgcctgca tcagcaccgg cagggcgtcg ccctgagtgc tggcccggcc atcgatcagc
     4021 gcctgcagca gctgctccat ttcctcggcc agcagcgcag cgccgtagaa ctcgaccatc
     4081 tgcagggtgc cctgcacctg atggacgtag ctcaggcaga aacgcaggcg cgtcagatcc
     4141 tgcgaactct cgacgaacgc ttccagggcc tggcgggcct gcttgagggt ttccgcgatt
     4201 tcgcccttga cccaatccag ggcgacatag tcgtgccgat cacccataca gactccacac
     4261 attgatcatg attacccctt tcgggtaaag gccagcactt gctcatcggc aaccggcaac
     4321 agcaggggat gactccagtc ggccacttcg ccaactccaa tcaccagcag tccgccgggg
     4381 gccagacgct cgaccaggcg cgcaagaatg tcgcgccggc gccagcgccg aaagtagatc
     4441 agcaggttct gacaaaagat gacgtccatg ccgcgctgcg gcgcacgcgc cagatcgagc
     4501 acattcagac ggacaaaaca ggtacgttca cgcagggcct cgcagacctg gaaaccgcca
     4561 tcgggctgtt ccaggaagta gcgctcgagc aactcgccgc ccacctcgcc gacccgccgc
     4621 gcgccgtaac ggccactgcg ggcacgggcc agggcgttga ggctgatatc ggtcccggtg
     4681 acgccgaagc gtccgctcag gcccgcctcg cgctgcacct cggcggccac cagcgccagc
     4741 gaataggcct cctcgccgct ggcgcagccg acactccaca gctgccaggc acgcccttcc
     4801 tgcaccacgc ccaggcgctc gcgcagatag acggccagat gctggaacga cgggggatga
     4861 cggaagaagt gggtttcctg caccgtcagg tggtccatca ggttcgacca ctcgaccgca
     4921 ccgcgcgggc cgtcggttac ctgctggaaa taactggcgt agctatccag ccccagttca
     4981 cgcagccggg ttcccagaat ggtctgcagg aacgcgcggc gctgctcgtt gagcaccatg
     5041 ccgctacgcg tttccagcaa ctcctgccag gccctgaatt ccgccgccgt gatatcgggc
     5101 agcggcttca tggcccagac cgcgcttggc tgcatgccgc gcccctcgct cgtggtcact
     5161 tgacggcagc ccgttcaggc tgccgcccac cgtggtatca ggcctgttcc cggtgttcgg
     5221 gcagggtgaa gccggaaacc gacttgcgca tgtcggtggc catcttggcc aaggtgccga
     5281 tgctgcgcgc ggttgcggtg gtacccgagg aggtctgcgt ggtgatctcc tggatcacgt
     5341 acatggtgtt ggagatgtga ccggccgagg aggactgctg gcgggcggcg ttggaaatgt
     5401 tctggatcag cgctgccagg ctgcgcgata ccttctcgat ctcttccagg gccacaccgg
     5461 cgtcctgtgc cagacgggca ccgcgcacca cctcggtggt ggtctgttcc atcgagatga
     5521 ccgcctcgtt ggtgtcggtc tgaatggtct tcaccagcgc ctcgatctgc ttggtcgccg
     5581 ccgaggagcg ttctgcgagg cgctgtacct cgtccgctac cacggcgaag ccgcgaccgg
     5641 cgtcaccggc catggaggcc tggatcgccg cgttcagtgc caggatgttg gtctggtcgg
     5701 caatgtcgtt aatcaggctg acgatgtcac cgatctcctg ggacgattcg ccgaggcgct
     5761 tgatccgctt ggaggtgtcc tggatctgct cgcggatgtt gtccatgccg gcgatggtgt
     5821 tctgcaccac ctcgttgccc ttgttggcga tggctaccga gcgctccgct accgcggagg
     5881 attcggcggc gttggccgac acctggtcaa tggacaccgc catctcgttg attgccgcgg
     5941 aagcaccggc aatttcctgg gcctggtgct cggaagcctc ggccaggtgc atcgccgtcg
     6001 cctgggtttc ctgggcggcg gcagccacct gcacggcggt ctggttgatg gtctctacca
     6061 gatcgcgcag ctggtcgatg gagtagttga tggagtcggc gatggcaccg gtgaagtcct
     6121 cggttaccgt ggcctgcacg gtcaggtcgc cgtccgccag gtcgccgatt tcatcgagca
     6181 gacgcagaat cgccgactgg ttgcggtcgt tcttctcggc ggtttccacc aggcggtcgc
     6241 gggtctcgcg caccaggatc agcgccatga gcatgatcga ggccagggcg agcaggccca
     6301 gcaggtagcc ggccagggta ttgaaggtcc ggctgacggc caggttctcg aaaccgctgg
     6361 ccagttgggt ggagccctcc agcaccttct gcgattcgcc gaagatgttg ttggcggact
     6421 cgcgcacctg gaacagttcc ggcgaggttt ccaggatctc gtccaccgag ccggaaacga
     6481 aggagaacag ttcggtgatc tccttcagac gggtcatggc ttcggcatcg gtcaccttgg
     6541 tgatcgacat ggccgggttg ccttcgtgca tgcccgtcag tacgcgaccg aacaggctgg
     6601 cgtcgcgacc gaagctgtcg gccgcctgca ccgagtcttc ggcaccggcg agcaccttgt
     6661 tcaccgcggc gagaatccgt tcggccagca gggactggcg ctgggccacg gccacctgat
     6721 cggccggcgc accgttctcc agcaggatat ccacgacctc ttcgtactcg acctgcagct
     6781 gcggaatggt ttccgccagg gtgctggcca cctggtgcag cgacagcacg gtctgctggc
     6841 tggccagaat ggcgtcggta ctggtgcgca ggcgctccca gtcggcctgc acggtggcga
     6901 ggttgctggc caccgcttcc ggactggccg gcaagccggt atcggcgtca ccttggctca
     6961 gccactccca gcgctgctgg aagtcgtcac gctcgctctt cagcagggcg aaggcgtcgg
     7021 ccttgcccgc cgccgcctcg acggcgttct tggcgatccc ctgggacagc aggcgcagct
     7081 caccggcgtg agcgatgtac tgcttgtcgt attcggactg ctggttgagg tacgcaaagt
     7141 tggcaaacag cagcaccaca gacacgatca ggatggcgaa aagaccaccg atcagactgc
     7201 tgctgcgtgt gcccgagaag agattgcctg cgttgagttt tttcatcttc tggcccccgc
     7261 ctggaccttc cacactaaac tgttcccaag taacgatacg ggccggcaat gccgccccat
     7321 ccggtagatc tagttattta cggcaacatc gaggaacgcc tgcgccctgg ccaacgcatg
     7381 cgggctgaac accagccagg ggcgctcacg ctggaacacg ccatggatga acggctggat
     7441 ggaggcttcg agcggcggca gctgctcggc aaaggcctcc acggagaagt gctgcatacc
     7501 gaacacttca tcgacgatga ggccggcaaa gacctcaccg tgctcgacca ccagcacccg
     7561 ccgctgcttg cgcagcggcg acagttcgct accgaagaaa ccacacaggt ccatgatcgg
     7621 cagcagccgg ccgcgcacgt tggcaacgcc cttgacccag cttctgacgc ccggcaacag
     7681 ggtgtagcgc ggctcgtgca ggacctcggt gacctcgccc atgggggcga cgaacaggcg
     7741 cgtgcccatg cgaaagccga tgccactcca gctctgggcg gcctcctgct gggctggcag
     7801 gcccgcggaa tagcggcggc agagctggtc gatctcgatc aggtgctgga aaggtgtcat
     7861 gacgtcggac atgccgctgc tggccttttt ctgcttgtcg cgtcctgcga tgaatgagct
     7921 atcagcccgc ctgaacggca gcgatggtct tcagcagggt ttcttcgtcg accggtttgg
     7981 tcaggtagtc gcgggcgcct tggcgcttgc cccagaccct atcggtttcc tgatccttgg
     8041 tggtgacaat gatcaccgga atgtgaccgg tttccgcgtc cttggtcagc tggcgggttg
     8101 cctggaaacc gttgaggccc ggcatgacca cgtccatcag cacgacatcg ggtttttcct
     8161 ggcgcgcgag ggcaacacca tcggcaccgt tttccgcctt caacacctga tgcccatgct
     8221 tctcgagcat ggcggtgagc ttgtacatct cggtcggcga gtcatcaaca atcagaattc
     8281 gagccatgaa attccccaat aaaggcacgc gtcggcagcc agtcggccgg acgtcaggtt
     8341 gtagcttgct ccaccggcac gaagcccgga acgtgcgtct tgatggcgcc gagcagttct
     8401 tccttgctga agggcttggt cagatactga tcggagccga cgatgcgccc cttggccttg
     8461 tcgaacaggc catccttgga ggacagcatg atcaccggcg tggatttgaa cgcactatta
     8521 ttcttgatca gggcgcaggt ttgatagcca tccagacgcg gcatcatgat gtcgacgaag
     8581 atgatgccag gatgcgaatc ggcgatcttg gccagggcat cgaagccgtc gacggccgtg
     8641 atgacgtcac aacccacttt cttcagcagg gtttcggcag tacgacgaat cgttttcgaa
     8701 tcgtcgatga ccatgacctt caagctctcg gaatgctgtt ccatgtctgc cctaccattg
     8761 cctcggtgag tcgttgtttt ccatcgcccg acgcgcttgc gacgctgcgt catgccgcag
     8821 ggtgcgacct gatcgacggg gctttttagc acactctccg gcggcaagcc ataagccccg
     8881 gatgcgtaag gtttttcctt gactcaaggc aaactcgcgg ccaccttggg caaccgtcaa
     8941 tcgccttgta cggccgtcag gccgttgttt cgttgcagag gacttcccct atgagcgttc
     9001 gcctgggcat cgtcatggac cccatcgccc gcatagcctt caagaaggac agctcgctgg
     9061 ccatgctgtt cgccgcccaa gcccgcggct ggtcgctgtt ctacatggaa cagcaggacc
     9121 tctacctgcg cgacggccag gtgcgggccc gcacgcgccc gctgcaggtc atccatgatg
     9181 ccaagcattg gttccatttc gatgacgagc aggatctggc cctgcacgag ctggacgtgg
     9241 tcctgatgcg caaggacccg ccgttcgaca acgaattcct ctattgcacg caactgctgg
     9301 agcaggccga gcgcgaaggc ttgctggtgg tcaaccgccc gcagagcctg cgcgactgca
     9361 acgagaaact gttcgccacc cagttcccgc aatgcacgcc gccgaccctg gtgagccggc
     9421 gcgtcgacat tctgcgcgag ttcgccgccg agcagcgtga catcattctc aaacccctgg
     9481 acggcatggg cggctcgtcg atcttccacc accgcgcagg cgatcccaac ctgtcggtga
     9541 tccttgagac gctgaccggc cacggcagcc gccagatcat ggcgcagcgc tacctgccgg
     9601 cgatcaagga cggcgacaag cgcatcctga tgatcgatgg cgagccgatt c
//
LOCUS       DATCUN010000111        11938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_4636, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000111 DATCUN010000000
VERSION     DATCUN010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11938
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4636"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..685
                     /locus_tag="VIO83_04290"
     CDS             29..685
                     /locus_tag="VIO83_04290"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003303307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY8330528.1"
                     /translation="MIHADNLSRPALRRQLRACRRALTPTQQRQAARDLYRQLAQHPL
                     FRRARHIALYLPNDGEIDPLLLRREAWRRGKHIYLPLLAHWPRTHMVFQRLERGETLR
                     RNRFRIPEPQACPARQRRPWALDLLLLPLVGFDDGGGRLGMGGGFYDRALDYRLRHRN
                     WQKPVLLGLAHACQQVDRLPLESWDVPLHATVTDRGWHAGCRRGELEEARSGRGLRRS
                     "
     gene            complement(753..887)
                     /locus_tag="VIO83_04295"
     CDS             complement(753..887)
                     /locus_tag="VIO83_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330529.1"
                     /translation="MHRKKPDMLWVLVFLFGLGVVTTSYAQSLWPKPGDVPAEYSRQP
                     "
     gene            complement(1095..2075)
                     /locus_tag="VIO83_04300"
     CDS             complement(1095..2075)
                     /locus_tag="VIO83_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HEY8330530.1"
                     /translation="MRITLQPSGLSLELLPGERILDAARRLGFDCPQSCRNGNCHVCA
                     ALLVEGRVRQQGAELDHGELFTCLAEPLEDCVLHWDGVLAPGELPVRQLACQLVECQA
                     VGGDVFRLRLRAPAGKPPRYHAGQYLLLEREDGSQAAFSIASAPHAGRELELHILARD
                     EGTRSLLDFIRRQGLARVQLPFGDCHLAELPDGPLLLLAAGTGLAQMHSLIEHCRAAG
                     FAHPVHLYWGVRQAQDFYRLPVWEEWRQLANLILHQVVSDPQPGDGWSGRCGLLHQAV
                     CADFADLKPLHVYASGSPAMVYGTLDALVAAGMDAHQMRADVFAYAPRPA"
     gene            complement(2075..3541)
                     /gene="ubiD"
                     /locus_tag="VIO83_04305"
     CDS             complement(2075..3541)
                     /gene="ubiD"
                     /locus_tag="VIO83_04305"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEY8330531.1"
                     /translation="MQYRDLREFIRGLEARGELKRIQTPVSPVLEMTEICDRTLRKQG
                     PALLFENPTGFSTPVLGNLFGTPKRVALGMGAEEVSELREIGKLLAFLKEPEPPKGLK
                     DAWSKLPIFKKVISMAPKVLKDAPCQEEIIAGDDVDLGQLPIQHCWPGDVGPLITWGL
                     TITKGPNKERQNLGIYRQQVIGRNKVIMRWLSHRGGALDYREWCQKYPDRPYPVAVAL
                     GADPATILGAVTPVPDTLSEYAFAGLLRGQRTELVKAVGSDLQVPAFAEMVLEGHIYP
                     GEMADEGPYGDHTGYYNEVDRFPVFTVERITRRRDAIYHSTYTGRPPDEPAILGVALN
                     EVFVPILQKQFPEIVDFYLPPEGCSYRMAVVTIKKQYPGHAKRVMLGVWSFLRQFMYT
                     KFVIVTDDDINARDWNDVIWAITTRMDPKRDTVMIDNTPIDYLDFASPISGLGSKMGL
                     DATHKWPGETTREWGRAIVQDEAVKRRVDELWSSLGID"
     gene            complement(3747..5006)
                     /gene="rho"
                     /locus_tag="VIO83_04310"
     CDS             complement(3747..5006)
                     /gene="rho"
                     /locus_tag="VIO83_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY8330532.1"
                     /translation="MNLTELKQKPIPELLEMADQMGLENMARSRKQDIIFALLKKHAK
                     SGEEISGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTIIGKIR
                     PPKEGERYFALLKVDTINFDRPENAKNKILFENLTPLFANKRLKMEAGNGSKEDLTAR
                     VIDLCAPIGKGQRGLIVAPPKAGKTIMLQNIAANITRNNPECHLIVLLIDERPEEVTE
                     MQRTVRGEVVASTFDEPPTRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VIPSSGKVLTGGVDAHALEKPKRFFGAARNIEEGGSLTILATALVETGSKMDEVIYEE
                     FKGTGNMELQLDRKIAEKRVFPAININRSGTRREELLTGDEELQRIWILRKILHPMDE
                     IAAIEFLLDRLKDSKTNEEFFQSMKRK"
     gene            complement(5232..5558)
                     /gene="trxA"
                     /locus_tag="VIO83_04315"
     CDS             complement(5232..5558)
                     /gene="trxA"
                     /locus_tag="VIO83_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEY8330533.1"
                     /translation="MSEFITNVTDASFEQDVLKADSPVLVDYWAEWCGPCKMIAPVLD
                     EIAKDYQGKLKVCKLNIDENQDTPAKFGVRGIPTLMLFKNGNVEATKVGALSKSQLAA
                     FLDSNI"
     gene            5776..7278
                     /gene="ppx"
                     /locus_tag="VIO83_04320"
     CDS             5776..7278
                     /gene="ppx"
                     /locus_tag="VIO83_04320"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003150181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEY8330534.1"
                     /translation="MPYTPAEAFPLIAAIDIGSNSIHMVLARVDQGEIRLLDRLGEKV
                     QLAAGLDEQRNLDEAAMTRGLDCLRRYAQLIHGLPQGAVRIVATNALREARNRAVFIR
                     RAEELLGHQVELVSGREEARLIYLGVSHSLPDAEGKRLVVDIGGGSTEFIIGERFDSL
                     LRESQQMGCVSYSKRYFGDGKLSLARYAQAYTAARLELMSFEQAVRKLGWSEAVGSSG
                     SIRAISQALAAGGQGNGEITPAGLAWLKRKLLKCDSLAELAIDGVKADRQSILPAGLA
                     ILEATFDALGIDSMSHTEGALREGVLYDMIGRQSHEDVRERTLSALMERYHVDLEQAA
                     RVEAKALSILAQVAEPWGLNDEWHRELLSWGAKVHEIGMDIAHYHYHKHGAYILEHAD
                     LAGFARIDQQMLALLVRGHRRNIPVDRFAEFGEEGERLVRLCILLRFAILFHHIRGTQ
                     EMPEVTFHAGPRSLEAVFPAGWLEANPLTQADIEAEAQWLARVGYKLGGR"
     gene            complement(7346..9574)
                     /gene="ppk1"
                     /locus_tag="VIO83_04325"
     CDS             complement(7346..9574)
                     /gene="ppk1"
                     /locus_tag="VIO83_04325"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY8330535.1"
                     /translation="MNNPGLIDNELPEQPAALAPLPDAEEAAQLAVTEPLAGMEEEAG
                     ELLAPVPGLDDSSLYIHRELSQLKFNIRVLEQALDESTPLLERLKFLLIFSSNLDEFF
                     EIRVAGLKKQVAFARELAGADGLQPQQVLARISELVHEQVERQYAILNDVLLPALEQH
                     QIRFIRRRHWTAKIKTWVRRYFREEIAPIMTPIGLDPTHPFPLLVNKSLNFIVELEGI
                     DAFGRDSGLAILPAPRLLPRVIRIPEEVGGPGDNFVFLSSMIHAHADELFHGMSVKGC
                     YQFRLTRNADLSVDAEDVEDLARALRGELFSRRFGDAVRLEVADTCPQHLSDYLLKQF
                     GLAESEMYRVNGPVNLTRLFSITGLDSHRELQYAPLTPVIPKLLQSSDKLFNVVGKQD
                     VLLYHPFESFTPVVDMLRQAAKDPNVLAIKQTLYRTGANSEIVDALVDAARSGKEVTA
                     VIEIRARFDEESNLALAGRLQQAGAVVIYGVVGFKTHAKMMLILRRENGELVRYAHLG
                     TGNYHAGNARLYTDYSLLTSDDALCEDVSKLFSQLIGMGKSLRMKKLLHAPFTLKKTL
                     LDMIAREGQHASEGKPAHIIAKMNSLTDPKVIKALYKASQCGVRIDLIVRGMCCLRPG
                     IPGVSHNIYVRSIIGRFLEHSRAYYFFNGGDEQLYLSSADWMERNLDKRVETCFPLEG
                     KKLITRVKKELEAYLADNTQAWVLQSDGRYQRLQPTGNQNARNAQALMVEKLSSPALS
                     AR"
     gene            complement(9604..10617)
                     /gene="hemB"
                     /locus_tag="VIO83_04330"
     CDS             complement(9604..10617)
                     /gene="hemB"
                     /locus_tag="VIO83_04330"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY8330536.1"
                     /translation="MSFTPANRLFPATRLRRNRRDEFSRRLVRENVLTSDDLILPVFV
                     LEGENRREAVPSMPGVERLSIDLLLEEAAELVALGIPALALFPVTPLEKKSLLAEEAW
                     NPDGLAQRATRALRARFPELGIITDVALDPFTTHGQDGILDADGYVQNDVTVDALVKQ
                     ALSHAAAGAQVVAPSDMMDGRIQAIREALELADHPNVRIMAYSAKYASAYYGPFRDAV
                     GSAGNLGKGSKNTYQMDPANGDEALHEVAADLAEGADMVMVKPGMPYLDIVRRVKDEF
                     RAPTFVYQVSGEYAMHMAAIQNGWLSEAVILESLVAFKRAGADGILTYFAKRAAQLLK
                     NQS"
     gene            10867..11520
                     /gene="elbB"
                     /locus_tag="VIO83_04335"
     CDS             10867..11520
                     /gene="elbB"
                     /locus_tag="VIO83_04335"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HEY8330537.1"
                     /translation="MNKKVAVILSGCGVYDGAEIHESVLALLRLSQRGAQVQCFAPDI
                     PQHHVINHLTGAESAESRNVLVESARIARGQIRDVRELQAADFDALILPGGFGAAKNL
                     SDFALRGASCAVQADVLAAARGFAAAHKPIGLICIAPAMAARIYGAGVQCTIGHDPET
                     AAKLVDMGAEHIDCPVDDIVVDAQRKLVTTPAYMLAKSLAEAASGINKLVDRVLELA"
     gene            complement(11593..>11938)
                     /locus_tag="VIO83_04340"
     CDS             complement(11593..>11938)
                     /locus_tag="VIO83_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HEY8330538.1"
                     /translation="AFACVRLIVVPSGPDAADDYLVEHALPGELVICSDVPLADRLVK
                     KGVAALDPRGREFDERNMGERLAVRNLFTDLREQGQVGGGQAAYAEKDRQAFANALDR
                     LLGALARRSSPL"
BASE COUNT         1900 a         3961 c         4074 g         2003 t
ORIGIN      
        1 gacccagacc ggagcgtcgc acctcgccat gattcacgcc gacaacctgt cccgccctgc
       61 cctgcgccgc cagctgcgcg catgccgccg cgccctcaca ccgacccagc agcgccaggc
      121 cgcccgcgac ctgtatcgcc agctcgccca gcatccgctg tttcgccgcg cccgccacat
      181 cgccctgtac ctgcccaacg acggcgagat cgatccgctg ctgctgcgcc gcgaagcctg
      241 gcgccgcggc aagcacatct acctgccgct gctggcgcac tggccgcgta cgcacatggt
      301 gttccagcgc ctggaacgtg gcgaaacgct gcgtcgcaac cgcttccgca ttcccgagcc
      361 gcaagcgtgc cccgcgcgcc agcgccggcc ctgggcactc gacctgctgc tgctgccact
      421 ggtgggcttc gatgacggcg gtggccggct gggcatgggc ggcggcttct acgaccgcgc
      481 cctggattac cgcctgcgtc accgcaactg gcagaagccg gtactgctgg gactggcgca
      541 cgcctgccag caggtcgatc gcctgccgct ggagagctgg gatgtgccgc tgcacgcgac
      601 agtgaccgat cgtggctggc acgccggctg caggcggggg gagctggagg aagcgcgcag
      661 cgggcgaggc ctgcgccgca gctgaacatc cgcaggcagg gcagccgtgt tgccggccgc
      721 tgcaggcggc cgggcgtcgg ttcagaggag gatcagggct gccggctgta ctcggcgggc
      781 acgtcgcccg gcttcggcca caggctctgc gcatagctgg tggtgaccac ccccagaccg
      841 aacaggaaaa ccagaaccca cagcatgtcg ggtttcttgc ggtgcatcga ctgccactcc
      901 ttgcaggcaa gcttgaagtg cggatcgccg gtgcttctct ggcaacctgg cgagtagttt
      961 ggattcgagc gggcgcattc tccggcaacg cggcacggca tgcaatttgt ccctgcaacc
     1021 ggtggtcggc aaatgcgccg cctgcgaccg acagcacagg cagcgggtga ccggcgcctc
     1081 gcgcctggcg tgactcaggc cggccgcggg gcgtaggcga acacgtcggc gcgcatctga
     1141 tgggcatcca tgccggccgc caccagcgca tccagcgtgc cgtagaccat cgccggcgag
     1201 ccgctggcgt agacgtgcag gggcttgagg tcggcgaagt cggcgcacac ggcctggtgc
     1261 agcagaccgc agcgcccgct ccagccatcg cctggctgcg ggtcgctgac cacctgatgc
     1321 aggatcaagt tggccaactg ccgccactcc tcccacaccg gcagacggta gaaatcctgc
     1381 gcctggcgca cgccccagta caggtgcacc ggatgggcga agccggcggc ccggcagtgc
     1441 tcgatcaggc tgtgcatctg cgccagcccg gtgccggcag cgagcagcag cagcggaccg
     1501 tcgggcagct cggccaggtg gcagtcgccg aacggcagct gcacccgggc cagcccctgg
     1561 cgacggatga agtcgagcag gctgcgcgtg ccctcgtcgc gggcgaggat gtgcagctcc
     1621 agctcgcgcc cggcgtgcgg cgccgaggcg atggagaagg ccgcctgact gccgtcctcg
     1681 cgctccagca gcagatactg cccggcgtgg tagcgcggcg gcttgccggc cggggcgcgc
     1741 aggcgcaggc ggaacacgtc gccgcccacc gcctggcact cgaccagctg gcaggccagc
     1801 tggcgcaccg gcagctcgcc gggcgccagc acgccgtccc agtgcagcac gcaatcttcc
     1861 agcggctcgg cgaggcaggt gaacagctcg ccatggtcca gctcggcgcc ctgctggcgc
     1921 acccggcctt ccaccagcag cgcggcgcag acgtggcagt tgccgttgcg gcagctctgc
     1981 gggcagtcga agccgagccg gcgcgcagcg tcgaggatgc gctcgccggg cagcagttcc
     2041 aggctgaggc cggagggttg tagggtaata cgcatcagtc gatccccagg cttgaccaca
     2101 gttcgtcgac ccgtcgtttc accgcctcgt cctggacgat ggcacgcccc cactcgcgcg
     2161 tggtctcgcc cggccacttg tgggtggcgt cgaggcccat cttgctgccc aggccggaga
     2221 tcggcgaggc gaagtcgagg tagtcgatcg gcgtgttgtc gatcatcacc gtgtcgcgct
     2281 tggggtccat gcgcgtggtg atcgcccaga tcacgtcgtt ccagtcgcgg gcgttgatgt
     2341 cgtcgtcggt gacgatgacg aacttggtgt acatgaactg gcgcaggaag ctccacacgc
     2401 cgagcatcac ccgcttggcg tggcccgggt actgcttctt gatggtcacc accgccatgc
     2461 ggtaggagca gccttccggc ggcaggtaga agtcgacgat ctcggggaac tgcttctgca
     2521 ggatcggcac gaacacttcg ttgagcgcca cgccgaggat cgccggctcg tccggcggcc
     2581 gtccggtgta ggtgctgtgg tagatggcat cacggcgacg ggtgatgcgc tcgacggtga
     2641 acaccgggaa acggtcgacc tcgttgtagt agccggtgtg gtcgccatag gggccctcgt
     2701 cggccatctc gccgggatag atgtgccctt ccagcaccat ctcggcaaac gccggcacct
     2761 gcaggtcgct gccgaccgcc ttgaccagct cggtgcgctg cccgcgcagc aggccggcga
     2821 aggcatactc ggacagcgtg tccggcaccg gagtgaccgc accgaggatg gtcgccgggt
     2881 cggcaccgag ggccaccgcc accgggtacg ggcggtccgg gtacttctgg caccactcgc
     2941 ggtagtccag cgcgccgccg cggtggctga gccagcgcat gatcaccttg ttgcggccga
     3001 tcacctgctg gcggtagatg ccgagattct gccgctcctt gttgggcccc ttggtgatgg
     3061 tcagccccca ggtgatcagc ggcccgacgt cgccgggcca gcagtgctga atcggcagct
     3121 ggccgaggtc gacgtcgtcg ccggcgatga tctcttcctg gcagggcgca tccttgagca
     3181 ccttcggcgc catgctgatg accttcttga agatcggcag cttggaccag gcgtccttca
     3241 gccccttggg tggctccggc tctttgagga aggccagcaa cttgccgatc tcgcgcagct
     3301 cggagacctc ctcggcaccc atgcccagcg ccacgcgctt gggcgtgccg aacaggttgc
     3361 cgagcaccgg cgtggaaaaa ccggtcggat tctcgaacag cagcgccgga ccctgcttgc
     3421 gcagggtgcg gtcgcagatt tcggtcattt ccagcacggg cgagaccggc gtctggatgc
     3481 gcttgagttc gccgcgcgcc tccaggccgc gaatgaattc gcgcaggtcg cgatactgca
     3541 tgcttgagcc tcgggaagaa tcgaaggcgg agagtctagc gccgaacggc accggacaga
     3601 aacgcgagaa gccgcctgta ggggcgaatt cattcgcctt ggcgaaacct gcaggcgaat
     3661 gaattcgccc ctacacccgc accaccgggc agaaacgaca acggccggga tggccggccg
     3721 ttgtctgcag atccagggag aaacgcttac ttgcgcttca tcgactggaa gaattcctcg
     3781 ttggtcttgg agtccttcag acggtcgagg aggaactcga tcgcggcgat ctcgtccatc
     3841 ggatggagga tcttgcgcag gatccagatg cgctgcagct cttcatcgcc ggtcagcagt
     3901 tcttcgcgac gggtaccgga tcggttgatg ttgatcgccg ggaacacgcg cttttccgcg
     3961 atcttgcggt ccagctgcag ttccatgttg ccggtgccct tgaattcctc gtagatcacc
     4021 tcgtccatct tcgagccggt ttccaccagc gcggtggcga ggatggtcag gctgccgccc
     4081 tcctcgatgt tgcgcgcggc accgaagaag cgcttgggct tctccagggc gtgggcgtcg
     4141 acaccgccgg tgagcacctt gccggagctg gggatcacgg tgttgtaggc gcgcgccaga
     4201 cgggtgatgg agtcgagcag gatgaccacg tccttcttgt gctcgaccag gcgcttggcc
     4261 ttctcgatca ccatctcggc gacctgcacg tggcgggtcg gcggctcgtc gaaggtcgag
     4321 gcaaccactt cgccgcgcac ggtgcgctgc atctcggtca cttcctccgg gcgctcgtcg
     4381 atcagcagga cgatcaggtg gcactcgggg ttgttgcggg tgatgttggc ggcgatgttc
     4441 tgcagcatga tggtcttgcc cgccttcggc ggagcgacga tcaggccgcg ttggcccttg
     4501 ccgatcggcg cgcacaggtc gatcacgcgg gcggtgagat cctctttgga gccgttgccg
     4561 gcttccatct tcaaccgctt gttggcgaac agcggggtga ggttctcgaa caggatcttg
     4621 ttcttggcgt tctccggacg gtcgaagttg atggtgtcga ccttgagcag cgcgaagtag
     4681 cgctcacctt ccttgggcgg acggatcttg ccgatgatgg tgtcgccggt acgcaggttg
     4741 aagcggcgga tctggctggg cgagacgtag atgtcgtccg ggccggccag gtaggaggag
     4801 tccgcggagc gcaggaatcc gaagccgtcc tgcaggatct ccagcacgcc atccccggag
     4861 atctcctcac cgcttttcgc gtgcttcttc agcagggcga agatgatgtc ctgcttgcgc
     4921 gaccgggcca tgttttccag gcccatctgg tcggccattt ccagcagttc gggaatcggc
     4981 ttttgcttga gttcggtcag gttcatagga tgaggtgcat cggggagaga aggaaagcat
     5041 tgagctcggg aggccgcgcc gcagggagac ggcaggttca ctgcgagaat ccggggcgag
     5101 agggtggcgg cgacggcatg caggaagcag tagatatgac tgccgcccga atttatcact
     5161 gcattcgtgg cacagtctag acggcaaaca gaaaaaaccc cgcaccaggc ggggtttgtc
     5221 gcacatcagg ttcagatgtt gctgtcgagg aaggcggcca gctgggactt ggacagggcg
     5281 ccgaccttgg tcgcttcgac gttgccgttc ttgaacagca tcagggtcgg gataccgcgc
     5341 acgccgaact tggccggcgt gtcctggttc tcgtcgatgt tcagcttgca caccttgagc
     5401 ttgccttggt agtccttggc gatctcgtcc agcaccgggg caatcatctt gcacggacca
     5461 caccactcgg cccagtagtc gaccagcacg gggctgtcgg ccttgagcac gtcctgctcg
     5521 aagctggcgt cggtcacgtt ggtgatgaat tcgctcatgg ggaatctccg taattcggat
     5581 gcgacaaaaa gttgcgtcat catagcccca gtcgccggcc accggaaggc ggcgggcgat
     5641 tggcaatcgc tatggtgcgc agcggcggcg tctatggccg gtggcggcgc gcgcccctga
     5701 caaccgtcaa ctgcgtcaca cgcgattgct ctatcatggc agccttacgc cgttttgccg
     5761 cgagaagcat cgaccatgcc gtacaccccc gccgaagcct ttcccctgat cgccgccatc
     5821 gacatcggct ccaacagcat ccacatggtc ctggcgcgcg tcgaccaggg cgagatccgc
     5881 ctgctcgacc gcctcggcga gaaggtccag ctggccgccg gcctcgacga gcagcgcaac
     5941 ctcgacgagg cggcgatgac ccgcggcctc gactgcctgc gccgctacgc ccagctgatc
     6001 catggcctgc cgcagggcgc ggtgcgcatc gtcgccacca acgccctgcg cgaggcgcgc
     6061 aaccgtgcgg tgttcatccg ccgcgccgag gagctgctcg gccatcaggt ggagctggtc
     6121 tccggtcgcg aggaggcgcg cctgatctac ctcggtgtgt cgcacagcct gccggacgcc
     6181 gagggcaagc gcctggtggt cgacatcggc ggcggcagta ccgagttcat catcggcgag
     6241 cgcttcgact cgctgctgcg cgagagccag cagatgggct gcgtcagcta cagcaagcgc
     6301 tacttcggcg acggcaagct gagcctggcg cgctacgccc aggcctacac cgccgcacgc
     6361 ctggagctga tgagcttcga gcaggcggta cgcaagctcg gctggagcga ggcggtcggc
     6421 tcctccggca gcattcgcgc catcagccag gcgctggcgg ccggcggcca gggcaacggc
     6481 gagatcaccc cggcggggct ggcctggctc aagcgcaagc tgctcaagtg cgacagcctg
     6541 gccgaactgg ccatcgacgg ggtcaaggcg gatcgccagt cgatcctgcc ggcgggcctg
     6601 gcgatcctcg aggcgacctt cgacgccctg ggcatcgact cgatgagcca caccgagggt
     6661 gccctgcgcg aaggcgtgct gtacgacatg atcggccggc agagtcacga ggacgtgcgc
     6721 gagcgcaccc tgtcggcgct gatggagcgc taccacgtcg acctggaaca ggccgcgcgg
     6781 gtggaggcca aggcgctgtc gatcctcgcc caggtcgccg agccctgggg gctcaacgac
     6841 gaatggcacc gcgagctgct gtcgtggggc gccaaggtgc acgagatcgg catggacatc
     6901 gcccactacc actaccacaa gcacggcgcc tacatcctcg agcacgccga cctggccggc
     6961 ttcgcgcgca tcgaccagca gatgctggcg ctgctggtgc gcggccaccg ccgcaacatt
     7021 ccggtggacc gcttcgccga attcggcgag gaaggcgagc gcctggtgcg cctgtgcatc
     7081 ctgctgcgct tcgccatcct gttccaccac atccgcggca cccaggaaat gcccgaggtg
     7141 accttccacg ccggcccgcg cagcctggaa gcggtgtttc ccgccggctg gctggaggcc
     7201 aacccgctga cccaggccga tatcgaagcc gaagcgcagt ggctggcgcg ggtgggctac
     7261 aagctgggcg ggcgctgagc caacggcgcc gcaacgaaaa agggcagctc cgcgaggggc
     7321 tgcccttttt ttgttggcgc gcggctcagc gtgccgacag cgccggactg ctcagcttct
     7381 cgaccatcag cgcctgggcg ttgcgcgcgt tctggttgcc ggtcggctgc aggcgctggt
     7441 aacgaccgtc gctctgcagc acccaggcct gggtattgtc ggccaggtag gcctccagct
     7501 ccttcttcac ccgggtgatc agcttcttgc cttccagcgg gaagcaggtc tccacgcgct
     7561 tgtcgaggtt gcgctccatc cagtcggcgc tggacaggta cagctgctcg tcgccgccat
     7621 tgaagaagta gtaggcgcgg ctgtgctcga ggaaacggcc gatgatcgag cgcacgtaga
     7681 tgttgtgcga cacgccggga atgcccggcc gcaggcagca catgccgcgc acgatcaggt
     7741 cgatgcgcac gccgcactgg ctggccttgt acagcgcctt gatcaccttg ggatcggtca
     7801 gcgagttcat cttggcgatg atgtgcgccg gcttgccctc gctggcgtgc tggccctcgc
     7861 gggcgatcat gtcgagcagg gtcttcttca gggtgaaggg cgcgtgcagc agcttcttca
     7921 tgcgcaggct cttgcccatg ccgatcagct ggctgaacag cttggagacg tcctcgcaca
     7981 gcgcgtcgtc ggaggtcagc aggctgtagt cggtgtacag gcgggcgttg ccggcgtggt
     8041 agttgccggt gccgagatgg gcgtagcgca ccagttcgcc gttctcgcga cgcaggatca
     8101 gcatcatctt ggcgtgggtc ttgaagccga ccacgccgta gatcaccact gcgccggcct
     8161 gctgcaggcg accggccagc gccaggttgg actcctcgtc gaagcgcgcg cggatctcga
     8221 tcaccgcggt gacttccttg ccgctgcgcg cggcgtccac cagcgcatcg acgatttccg
     8281 agttggcccc ggtgcggtac agggtctgct tgatcgccag cacgttgggg tccttggccg
     8341 cctggcgcag catgtcgacc accggggtga acgactcgaa cgggtggtag agcagcacgt
     8401 cctgcttgcc gaccacgttg aacagcttgt cgctgctctg cagcagcttg ggaatcaccg
     8461 gggtcagcgg cgcgtactgc agctcgcggt gactgtccag gccggtgatg gaaaacagcc
     8521 gggtcaggtt gaccggaccg ttgacccggt acatttccga ttcggccagg ccgaactgct
     8581 tgagcaggta gtcggagagg tgctgcgggc aggtgtccgc cacctccagg cgcaccgcat
     8641 cgccgaagcg ccgcgagaac agctcgccgc gcagggcgcg ggccaggtcc tcgacgtcct
     8701 cggcgtccac cgacaggtcg gcgttgcggg tcaggcggaa ctggtagcag cccttgacgc
     8761 tcatgccgtg gaacagctcg tcggcgtggg cgtggatcat cgaggacagg aacacgaagt
     8821 tgtcgccggg gccgccgacc tcctcgggga tgcggatcac ccgcggcagc aggcgcggcg
     8881 ccggcaggat ggccaggccc gagtcgcggc cgaaggcgtc gatgccttcc agctcgacga
     8941 tgaagttcag gctcttgttg accagcaggg ggaacgggtg ggtcgggtcg aggccgatcg
     9001 gcgtcatgat cggcgcgatc tcctcgcgga aatagcgacg cacccaggtc ttgatcttcg
     9061 ccgtccagtg ccggcggcgg atgaagcgga tctggtgctg ctccagtgcc ggcagcagca
     9121 cgtcgttgag gatcgcgtac tggcgctcga cctgctcgtg caccagctcg ctgatccgcg
     9181 ccagcacctg ctgtggctgc aggccgtcgg cgccggccag ttcgcgggcg aaggcgacct
     9241 gcttcttcag cccggcgacg cggatctcga agaactcgtc gaggttgctg gagaagatca
     9301 gcaggaactt caggcgctcg agcagcggcg tggactcgtc gagcgcctgc tccagaacac
     9361 ggatattgaa cttcagctgc gacagctcgc ggtggatgta caggctgctg tcgtccagcc
     9421 ccggcaccgg ggccagcagc tcaccggcct cctcttccat cccggccagc ggctcggtca
     9481 cggccagctg ggcagcctcc tccgcgtcgg gcagtggggc gagcgccgcc ggctgttccg
     9541 gcagttcgtt atcgataagt cctggattgt tcattgcggg ttcctgcagg gccttaggcc
     9601 ccgttagctc tgattcttca acagttgggc cgcacgcttg gcgaagtagg tgagaatgcc
     9661 atcggcaccg gcacgcttga acgcgaccaa agactcgagg atcaccgcct cggacagcca
     9721 gccgttctgg atcgccgcca tatgcatggc gtactcgccg ctgacctggt agacaaaggt
     9781 cggtgcgcgg aattcatcct tcacccgacg gacgatgtcg aggtagggca tgcccggctt
     9841 gaccatcacc atgtcggcgc cttcggccag gtcggcggcc acctcgtgga gggcctcgtc
     9901 gccgttggcc gggtccatct gataggtatt cttgctgccc ttgccgaggt tgccggccga
     9961 gcccaccgca tcgcggaacg ggccgtagta ggcgctggcg tacttggccg agtaggccat
    10021 gatgcgcacg ttggggtggt cggccagttc cagcgcctcg cggatcgcct ggatgcggcc
    10081 gtccatcatg tccgacgggg ccaccacctg ggcaccggcg gcggcatgcg acagcgcctg
    10141 cttgaccagc gcgtcgacgg tgacgtcgtt ctgcacgtag ccgtcggcat cgaggatgcc
    10201 atcctggccg tgggtggtga aggggtcgag cgccacgtcg gtgatgatgc ccagctccgg
    10261 aaagcgcgcg cgcagggcgc gggtggcgcg ctgcgccagg ccgtccgggt tccacgcctc
    10321 ttcggcgagc agcgacttct tctccagcgg cgtcaccgga aacagcgcca gcgccgggat
    10381 gcccagcgcc accagctccg cggcctcctc gagcagcagg tcgatggaca ggcgctccac
    10441 ccccggcatc gacggcaccg cctcgcggcg gttctcgccc tccagcacga acaccggcag
    10501 gatcaggtcg tcgctggtca gcacgttctc gcgcaccagg cggcgggaga actcgtcgcg
    10561 gcggttgcgg cgcaggcggg tggcagggaa caggcgattg gcgggggtaa agctcacggc
    10621 gcactccagg gcccgggcga cgggcgcagc ttgacagtct atgaagcaat tatgacggaa
    10681 atgcgacagc ctccgggcac acagtccggg cgtctggcat tcctggccgc taccttagcc
    10741 cgtcggtgcc cttatcggcc accgacgcat ggccgcagtg gcccgcttgc agctaggatt
    10801 gtggctaacc gcttgcgcct gcgccggacg gcccgcgcag gcccacctgt ccgaggagtg
    10861 cccgaaatga acaagaaggt tgccgtgatc ctgtccggct gcggcgtcta cgatggcgcc
    10921 gagatccacg aaagcgtgct cgccctgctg cgcctgagcc agcgcggcgc gcaggtgcag
    10981 tgcttcgccc cggatatccc gcagcaccac gtgatcaacc acctgaccgg cgcggaaagc
    11041 gccgagagcc gcaacgtgct ggtggagtcg gcgcgcatcg cccgcggcca gatccgcgac
    11101 gtgcgcgaac tgcaggccgc cgacttcgac gcgctgatcc tgcccggcgg cttcggcgcg
    11161 gcgaagaacc tttccgactt cgccttgcgc ggcgccagct gcgcggtaca ggccgacgtg
    11221 ctggccgccg cgcgcggctt tgctgccgcg cacaagccga tcggcctgat ctgcatcgcc
    11281 ccggcaatgg ctgcgcgcat ctacggcgcc ggcgtgcagt gcaccatcgg ccacgacccg
    11341 gagaccgcgg ccaagctggt cgacatgggc gccgagcaca tcgactgccc agtggacgac
    11401 atcgtcgtcg atgcgcagcg caagctggtc accaccccgg cctacatgct ggccaagtcg
    11461 ctggccgaag cggccagcgg gatcaacaag ctggtcgacc gggtactcga actggcctga
    11521 tgggtcgagg ctgtaggagc agattcatct gcgacctgcc ctgccggggg cgggtcgcga
    11581 atgaattcgc ccctacagag ggctactgcg ccgcgccagc gcgccgagca ggcgatcgag
    11641 ggcattggcg aaggcctggc ggtccttctc cgcataggcc gcctggccgc cgccgacctg
    11701 gccctgctcg cgcaggtcgg tgaacaggtt gcgcaccgcc aggcgctcgc ccatgttgcg
    11761 ctcgtcgaac tcgcggccgc gcggatccag cgcggcgacg cccttcttca ccaggcggtc
    11821 ggccagcggc acgtcgctgc agatcaccag ctcgccgggc agcgcgtgct cgaccagata
    11881 gtcgtcggcg gcatccggcc cgctgggcac cacgatcagc cgcacgcagg cgaaggcc
//
LOCUS       DATCUN010000112        13692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_155104, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000112 DATCUN010000000
VERSION     DATCUN010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 13692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13692
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155104"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VIO83_04345"
     CDS             complement(<1..470)
                     /locus_tag="VIO83_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014595540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY8330539.1"
                     /translation="MPLPSLKQQFAELIALPSVSCTQASLDLPNRPLIERLAGWLSDL
                     GFACEIQDIAPGKANLLASYGSGPGGLVLAGHSDTVPFDAALWQSDPLALREADDRWY
                     GLGSCDMKGFFPLIIEAVKPLLERPFRRPLLVLATCDEESSMSGARALAAAGRPL"
     gene            complement(563..1828)
                     /locus_tag="VIO83_04350"
     CDS             complement(563..1828)
                     /locus_tag="VIO83_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY8330540.1"
                     /translation="MSLIADYGFVLLVLACAFGFFMAWGVGANDVANAMGTSVGSRAL
                     TIKQAILVAMVFEFCGAYLAGGEVTETIKNGIVDAEVISPDLMVLGMMSALLAAGTWL
                     LVATAKGWPVSTTHSIIGAVIGFAAVGVSMDAVQWSAIGPIVASWVVTPFLSGLVAFG
                     LFMSVQKLIIDTDEPFRNAKRFVPFYMFLTGFMVALMTVTKGLKHVGLHLSGNQGILL
                     ALGIGVLVMLLGMAILSRIQVDTEADKGFHYASVEKVFAVLMVFTACSMAFAHGANDV
                     ANAVGPLAGIVGVIQSGGDMAVGAKSAVPGWVLLLGAVGIVIGLATYGYKVIATIGKE
                     ITELTPSRGFAAELATASTVVGASAIGLPVSTTHTLVGAVLGIGMARGIGALNLGVIG
                     KIFMSWIITLPAGAILAIIFFTILKAIFV"
     gene            complement(1866..2543)
                     /locus_tag="VIO83_04355"
     CDS             complement(1866..2543)
                     /locus_tag="VIO83_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HEY8330541.1"
                     /translation="MPINPFVSLFGRSPIGPMQQHIAKAHECAANLLPFFQAVIAEDW
                     PRVEEIQQEMSRLEQEADKLKKSVRIHLPKSLFLPVPRSDLLELLSVQDKVANRAKDI
                     AGLMLGRQMTIPQAMQPLMLAYVQRTVDASAQALKAMNELDELLETGFGGREVTLVEN
                     MVEELEVIEQDTDRLQIEVRRTLFKLEKDLPPVDVMFLYQIIEWIGDVADRAERVGNR
                     LEQLLAR"
     gene            2656..4017
                     /locus_tag="VIO83_04360"
     CDS             2656..4017
                     /locus_tag="VIO83_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HEY8330542.1"
                     /translation="MVKETEIKLRASRATLAALREHPLLKKRNKSGWQQSELFNQYFD
                     TPERDLAQARVALRLRRDGEQYIQTLKGRGQSVAGLSERNEWDWQLDKNKLDLKKLAA
                     ECWPAELAELDKKTLKPIFTTDFVREKADIAWGRGKAKVVIEAALDLGKVTAGKQSEE
                     ICELELELREGDPAALLELAAELAATLALMPCDISKAERGYRLFNPASYSLTLPEPQL
                     DAAMTLDDAFAALAWQLLGNSQRLAEQYRFNGHWKLLEQWLAQLIDLRALLGSLGQAA
                     PRASSRELREALDALLEDWRPHVQAGQDDDSVRQATPEQFAEELNDPRWGLFSLQTSR
                     WLLTRAWTEKRTERGARIGAAGIGRWLQRFLAEEAAALQLDRYQRQPEDLAEQLPRLE
                     RMLVWLRLARSVLDIPEADRLYGELNKLAELANRPLDEQTLQARTEQALTVFMLKGWK
                     ALK"
     gene            4161..5945
                     /locus_tag="VIO83_04365"
     CDS             4161..5945
                     /locus_tag="VIO83_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY8330543.1"
                     /translation="MTALSTPAQDRWIDLNDLLRELVAQGRLDQDSAEQCLAIRRSTL
                     NNQQHPLEFLAAQQVDDRAHPGKKLDLEGLTQWLAQFAGQPYLRIDPLKIDVAALTPL
                     MSYAFAQRHKILAVAVDSESVTIASAQPFVASWEANLTHVLKRPIKRVVANPEDIQRF
                     TTEFYRLARSVSGAAAGEQKISGVGNFEQLLNLGAADQEPDANDAHIVTIVDWLFQYA
                     FQQRASDIHIEPRREQGTVRFRIDGVLHNVYQFPPQVTMAIVSRLKSLGRMNVAEKRK
                     PQDGRVKTKAPDGGEIELRLSTLPTAFGEKLVMRIFDPEVLLKGFDQLGFSADDLRRW
                     QSMTGQPNGIILVTGPTGSGKTTTLYTTLKQLATEEVNVCTIEDPIEMIEPAFNQMQV
                     QHNIDLTFAGGVRALMRQDPDIIMIGEIRDLETAEMAIQAALTGHLVLSTLHTNDAPS
                     AISRLLELGVPHYLLKATILGVMAQRLVRTLCPHCKAPHAIDAADWQELTRPWNAPLP
                     SNAMRAVGCNECRDTGYRGRAGVYEIMLLSEGLKQQIQPDTELTGLRRQAFKEGMRSL
                     RLSGAQKIAAGLTTMEEILRVTPQSEQR"
     gene            complement(6043..6477)
                     /locus_tag="VIO83_04370"
     CDS             complement(6043..6477)
                     /locus_tag="VIO83_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY8330544.1"
                     /translation="MLRNQPSLSLDLAMLALPAVLAEARSLRVRISVSIVDAAGQLIH
                     LAHMDGAPALSRDIATDKAYTAASFGLATSRWEAELAGAPASVRNGLAHRPRMVLFGG
                     GVPVKVDGAVVGAIGVSGASAEQDEQCAQAGVNAILAALNQA"
     gene            complement(6537..6989)
                     /gene="hpaR"
                     /locus_tag="VIO83_04375"
     CDS             complement(6537..6989)
                     /gene="hpaR"
                     /locus_tag="VIO83_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HEY8330545.1"
                     /translation="MHKPHPSLIQALLQAREATLGFFRPLLNQYELTEQQWRVIRILS
                     QHRRDELESHQLAEQACILRPSLTGVLVRLERDGLVRRWKPASDRRRLCVSLTDKGVA
                     LFEEMSSEMTRLYKDIQRQFGTENFRTLMRLLQDLGKIDPGTQGGHSA"
     gene            complement(7089..7889)
                     /gene="hpaI"
                     /locus_tag="VIO83_04380"
     CDS             complement(7089..7889)
                     /gene="hpaI"
                     /locus_tag="VIO83_04380"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017528296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HEY8330546.1"
                     /translation="MQMPVNTFKQRLMTEQQIGLWIGLADPYCAELAANAGFDWLLLD
                     GEHAPNDLRSLLGQLQAVAPYASQPVIRPPIGDPVLIKQLLDIGAQTLLVPMVERAEQ
                     AEELVAAMHYPPKGIRGVGASLARASRWNNITTYLDETDAQMCLLVQVENLKGLENLD
                     AIAAVEGVDGVFIGPADLSAAMGHRGNPGHPEVQAAIEDAIARVRKAGKAAGILSGDE
                     KLARRYLELGCSFVAVGVDSSVLMRSLKRLANSFKGIESAPSVSGSVY"
     gene            8298..9509
                     /locus_tag="VIO83_04385"
     CDS             8298..9509
                     /locus_tag="VIO83_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017526993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HEY8330547.1"
                     /translation="MPPVVANPQRLIVLLAALVAFGPLSIDMYLPSLPLIARDLGAPE
                     AQIQLTISVFLAGLCTGMLVYGPLSDRFGRRKLLLGGIVLYMVASLGCILAAKAEHLV
                     FWRILQALGGAGASVLARTIVRDLFPLKDAARVLSLMHLVTMFATLIAPLIGGYLILL
                     AGWRSLFMVLMAFAGLCLLAVVWKIPETHPAHARGASVGAVFRAYGYIAVQPQALGYI
                     LCMGLSFGGMFAFITASPFVYIQYFGVSPQQYAWLFSLNVFGIIVATLLNARLVGRLG
                     PQRLLVCGASIAALSGLGLAILGSSGVGGLTAIVVCLLFYVSVTGLLGANCVASLLSH
                     FPKQAGAAAGLAVASQFGLGTAASALVSALHDGSPLPMSLVVGATGVGSLLALLLTRI
                     PERAAASAQGK"
     gene            complement(9777..13499)
                     /locus_tag="VIO83_04390"
     CDS             complement(9777..13499)
                     /locus_tag="VIO83_04390"
                     /inference="COORDINATES: protein motif:HMM:NF042738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6701 domain-containing protein"
                     /protein_id="HEY8330548.1"
                     /translation="MLGWVVSAWAGLGINADNFPNGIQSHTTNGKVTFGWNGVLETDN
                     QQVRAGSIAQNLSQPTPTCGASGFCQAISPAAPKQDAGSFRSASGPLPSVNVGYRGSA
                     AVNANGSNEYKNWDVGQEATLTVNGNGETFFIDKLDLAYRATLVLGGGDYWIDEIKFS
                     GTEVKIIIPAGETVRIFARKSFSVSFKSIMNYRDAATPGLPEQLFIYAYNDFTVDNQS
                     LVPGYIYSKGKVTIGNAATWKGAITADDVTLESSGARVIYAAPPEPPAPQTCLADDFN
                     RLALGSSDWAVTSRNGSFGAPRIVNNRLRLTDSSGNVATGATIQRLVPATANFVQVQF
                     KYYAYNGSGADGVAMILSDATQTPQPGGYGGSLGYAQNGSISGFAGGWLAVALDEYGN
                     FSNPTEGRVGGPGFRQDSVSVRGSGSGTTGYRYLAGTAANLSPGIDISGSTAGPGHTY
                     RITLDNTVAGKSMLQVERNTGAGFVTLISSFDVLASSGQAALPANFFLTFTGSTGGSN
                     NIHELDDLQVCATQMNAIGQQIDHYEFVHAGNALTCNPLSVEVRACLNADCSLRHTDP
                     TSVTLSPSGWEGGSSRTFSGGTLVDNLRVTTAQTVTLGVSNSNPPLKALSQTLCKVGA
                     APLSTNCQVAFADSGFIFDVPNLLARKEQTGIALQAVRKDNATQRCVPAFGPATRTLQ
                     FWSDYVDPGTGSMAVQVNDTAVSGNAASPTGLSLVFDNQATTQLKVRYDDAGLMQLNA
                     RFDGAGLESGLVMTGADQFVSKPYGLHISTPADTSCTVASVSGCSMLRAAGDSFPLSI
                     RAVAWQADGEALTAAALSDNPTTPNFQLNGIALNSMLVEPSVASGGVAGTFYRHAADG
                     TRQAALISYDHAVGASTTLQVGQSEVGIYRITATPPAGTYHTLTVSGGESALIGRFTP
                     AYLGATSTASLEPACGAFSYQGQTINFSAGPPGIVVTGYNRQGAVTNNYDRDPFWRLE
                     APQRQAYSFSDTSKPDLVSRLQSLGDPQSLAVADSGGADGRRVFDWRADGVRLADALL
                     WRLPSPPTAEDLPFVLTAAGDHVELRVGTSELTDLDGICYLGSAATCQDFVHAFGGTE
                     VRLGRLRIDAASGPENQALDLPYWLESWQASAGGNAFGATTDDSCSLAALGDVVLSDF
                     TGYLQASDFPAPPGTLAAPPASTPTPTGVIRLPAPNKKGSVLASLSGLNGATPALPWL
                     LFDWDGNGTPEAPSALATFGVLSSQRALIFRREIYR"
BASE COUNT         2351 a         4633 c         4482 g         2226 t
ORIGIN      
        1 agcgggcggc cggcggcggc cagggcgcgc gcgccgctca tcgagctttc ctcgtcgcag
       61 gtggcgagga ccagcagcgg ccggcggaac ggccgctcga gcagcggctt gaccgcctcg
      121 atgatcagcg ggaagaagcc cttcatgtcg cagctgccca ggccgtacca gcggtcgtcg
      181 gcctcgcgca gggccagcgg atcgctctgc cacagcgcgg catcgaaggg cacggtatcg
      241 ctgtgcccgg ccagcaccag gccgccgggg ccggagccgt agctggccag caggttggcc
      301 ttgccggggg cgatgtcctg gatctcgcag gcgaagccga gatcgctcag ccagccggcc
      361 aggcgctcga tcagcggacg attgggcaaa tcgagggagg cctgggtgca actcaccgac
      421 ggcagggcga tcagttcggc gaactgctgc ttgagactgg gaaggggcat gacggcactc
      481 cggaaacagt gccttgcatg ataggccggg aggcccgagg gggaaaccgc cgcgcccgca
      541 ccaagcggac gcggcggctg gctcagacga agatcgcctt gaggatggtg aagaagatga
      601 tcgccaggat ggcaccggcc ggcagggtga tgatccacga catgaagatc ttgccgatca
      661 cgccgaggtt cagcgcaccg atgccgcggg ccatgccgat gccgagcacg gcgccgacca
      721 gggtgtgggt ggtggacacc ggcaggccga tggccgaggc gccgaccacg gtggacgcgg
      781 tggccagctc ggcggcgaag ccgcggctcg gggtcagctc ggtgatctcc ttgccgatgg
      841 tggcgatcac cttgtagccg taggtggcca ggccgatgac gatgccgacg gcgccgagca
      901 gcagcaccca gcccggcacc gccgacttgg cgccgaccgc catgtcgccg ccggactgga
      961 tcacgccgac gatgccggcc agcggaccga ccgcgttggc cacgtcgttg gcgccgtggg
     1021 cgaaggccat cgagcaggcg gtgaacacca tcagcacggc gaacaccttc tccacgctgg
     1081 catagtggaa acccttgtcc gcctcggtat cgacctggat gcggctgagg atggccatgc
     1141 ccagcagcat caccagcacg ccgatgccca gcgccaggag gatgccctgg ttgccggaga
     1201 ggtgcaggcc gacatgcttg aggcccttgg tcacggtcat cagcgcgacc atgaagccgg
     1261 tgaggaacat gtagaacggc acgaagcgct tggcgttgcg gaacggttca tcggtgtcga
     1321 tgatcagttt ctgcacgctc atgaacaggc cgaaggcgac caggccggag aggaacgggg
     1381 tgaccaccca gctggcgacg atcgggccga tggcgctcca ctgcaccgca tccatcgaca
     1441 cgccgaccgc ggcgaagccg atcaccgcac cgatgatcga gtgggtggtg gacaccggcc
     1501 agcccttggc ggtggccacc agcagccagg tgccggccgc cagcagcgcc gacatcatgc
     1561 ccagcaccat caggtcggga gagatgacct cggcatcgac gatgccgttc ttgatggttt
     1621 cggtgacttc accgccggcc aggtaggcac cgcagaactc gaagaccatg gcgaccagga
     1681 tcgcctgctt gatggtcagc gcgcgcgagc cgaccgaggt gcccatggca ttggccacgt
     1741 cgttggcgcc gacgccccag gccatgaaga aaccgaaggc gcaggcgagc accaggagta
     1801 cgaagccgta atccgcaata agagacatag gaagttctcg ttgatcctag acagaatgcc
     1861 gccgctcagc gcgccagcag ttgttccaga cggttgccga cccgctcggc gcggtcggcc
     1921 acatcgccga tccattcgat gatctgatag aggaacatca cgtcgactgg cggcaggtcc
     1981 ttctccagct tgaacagggt acggcgcact tcgatctgca ggcggtcggt atcctgttcg
     2041 atgacctcca gctcctcgac catgttttcc accagggtca cctcgcgccc gccaaaaccg
     2101 gtctcgagca gctcgtccag ctcgttcatc gccttcagcg cctgggcgct ggcgtcgacg
     2161 gtgcgctgca cgtaggccag catcagcggc tgcatggcct gcgggatggt catctggcgg
     2221 cccagcatca ggccggcgat gtccttggcg cggttggcga ctttgtcctg tacactgagc
     2281 agctccagca gatccgagcg tggcaccggc aggaacaggc ttttcggcag gtggatacgc
     2341 acgctcttct tgagcttgtc ggcttcctgc tccaggcgtg acatctcctg ctgaatctct
     2401 tccacccgcg gccagtcctc ggcgatgacc gcctggaaga acggcaggag gttcgccgca
     2461 cattcatgtg ccttggcaat gtgctgctgc atcggcccga tcggggagcg gccaaacagg
     2521 ctgacgaagg gattgatcgg catggggtaa aaccgtgttg aaaaggatcc cgattataaa
     2581 gccgcccctc gcagccccgc cagcgcgcgt tcatttagtt gtcacagttg tctgataagg
     2641 gcaggaccgc cagtcatggt caaagaaacc gaaatcaagt tgcgcgccag ccgcgccacc
     2701 ctcgccgccc tgcgcgagca cccgctgctg aagaagcgca acaagagcgg ctggcagcag
     2761 tccgagctgt tcaaccagta cttcgacacc cccgagcgcg accttgccca ggcgcgcgtc
     2821 gccctgcgcc tgcgccgtga cggcgagcag tacatccaga ccctgaaagg ccgcggccag
     2881 agcgttgccg ggctttccga gcgcaacgag tgggactggc agctggacaa gaacaagctg
     2941 gacctgaaga agctggccgc cgagtgctgg ccggccgaac tggccgagct ggacaagaag
     3001 accctcaagc cgatcttcac caccgacttc gtccgcgaga aggccgacat cgcctggggc
     3061 cgcggcaagg ccaaggtggt gatcgaggcg gcgctggacc tcggcaaggt gaccgccggc
     3121 aagcagagcg aggaaatctg cgagctggag ctggaactgc gcgaaggcga cccggccgcg
     3181 ctgctggaac tggccgccga gctggccgcc accctggcgc tgatgccctg cgacatcagc
     3241 aaggccgaac gcggctaccg cctgttcaac ccggccagct acagcctgac cctgcccgag
     3301 ccgcagctcg acgcggccat gacgctggac gacgccttcg ccgcgctggc ctggcagctg
     3361 ctcggcaaca gccagcgcct cgccgaacag taccgtttca acggccactg gaagctgctc
     3421 gagcagtggc tggcccagct gatcgacctg cgcgccctgc tcggcagcct cggccaggcc
     3481 gcgccgcgcg ccagctcgcg cgagctgcgc gaggcgctgg atgccctgct cgaagactgg
     3541 cgcccgcacg tgcaggctgg ccaggacgat gacagcgtgc gccaggccac tcccgagcag
     3601 ttcgccgagg agctgaacga tccgcgctgg ggcctgttct ccctgcagac ctcgcgctgg
     3661 ctgctgaccc gcgcctggac cgagaagcgc accgagcgcg gcgcgcgcat cggtgccgcc
     3721 ggcatcggcc gctggctgca gcgtttcctc gccgaggagg ccgccgccct gcagctggac
     3781 cgctaccagc gccagccgga agacctggcc gaacagctgc cgcgcctgga gcgcatgctg
     3841 gtctggctgc gtctggcgcg cagcgtgctg gacattcccg aggccgaccg cctgtacggc
     3901 gagctgaaca agctggccga actggccaac cgcccgctgg acgagcaaac cctgcaggcg
     3961 cgcaccgagc aggcgctgac cgtgttcatg ctcaagggct ggaaggcgct gaagtaagcg
     4021 cccgccactg aggctcccgt agggtgcgcc atgcgcaccc tacgggagcc gtccggccaa
     4081 aacatgaccc accccggcga cagcccgggg atttcccggc aagcttgccc ctcgcgccaa
     4141 ccgccagccc gaggaagccc atgaccgcct tgtccacccc cgcccaggat cgctggatcg
     4201 atctcaacga cctgctgcgc gagctggtcg cccaggggcg gctcgatcag gacagcgccg
     4261 aacagtgcct ggcgatccgc cgcagcacgc tgaacaacca gcagcatccg ctggaattcc
     4321 tcgccgccca gcaggtcgac gaccgcgccc atccgggcaa gaagctcgac ctggaaggcc
     4381 tgacccagtg gctggcgcag ttcgccggcc agccctacct gcgcatcgac ccgctgaaga
     4441 tcgacgtcgc cgcgctcacc ccgctgatgt cctacgcctt cgcccagcgc cacaagatcc
     4501 tcgccgtggc ggtggacagc gagagcgtga ccatcgccag cgcccagccg ttcgtcgcta
     4561 gctgggaagc caacctgacc cacgtgctca agcggccgat caagcgggtg gtggccaacc
     4621 ccgaggacat ccagcgcttc accactgagt tctaccgcct ggcgcgctcg gtcagcggcg
     4681 ccgcggccgg cgagcagaag atcagcgggg tcggcaactt cgagcagctg ctcaatctgg
     4741 gcgccgccga ccaggagccg gatgccaacg acgcgcacat cgtcaccatc gtcgactggc
     4801 tgttccagta cgccttccag cagcgcgcca gcgacatcca catcgagccg cgccgcgagc
     4861 agggcacggt gcgctttcgc atcgacggcg tgctgcacaa cgtctatcag ttcccgccgc
     4921 aggtcaccat ggccatcgtc agccggctga agtcgctggg acggatgaac gtcgccgaga
     4981 agcgcaagcc gcaggacggc cgggtgaaga ccaaggcccc ggacggtggc gagatcgagc
     5041 tgcgcctgtc gaccctgccc accgccttcg gcgagaagct ggtgatgcgc atcttcgacc
     5101 ccgaggtgct gctcaagggc ttcgaccagc tcggcttctc ggccgacgac ctgcgccgct
     5161 ggcagagcat gaccggccag cccaacggca tcatcctggt caccggcccc accggctcgg
     5221 gcaagaccac cacgctgtac accaccctca agcagctggc gaccgaggag gtgaacgtct
     5281 gcaccatcga ggacccgatc gagatgatcg agccggcgtt caaccagatg caggtgcagc
     5341 acaacatcga cctgaccttc gccggcggcg tgcgcgcgct gatgcgccag gacccggaca
     5401 tcatcatgat cggcgagatc cgcgacctgg agaccgccga gatggccatc caggcggcgc
     5461 tgaccggcca cctggtgcta tccacgctgc acaccaacga cgcgcccagc gccatcagcc
     5521 gcctgctcga gctgggcgtg ccgcactacc tgctcaaggc caccattctc ggcgtgatgg
     5581 cccagcgcct ggtgcgcacc ctgtgcccgc actgcaaggc gccgcacgcc atcgacgccg
     5641 ccgactggca ggagctgacc cggccgtgga acgcgccgct acccagcaac gccatgcgcg
     5701 cggtgggttg caacgagtgc cgcgacaccg gctaccgcgg tcgcgccggc gtctacgaga
     5761 tcatgctgct gtcggagggg ctcaagcagc agatccagcc cgacaccgaa ctcaccggcc
     5821 tgcgtcgcca ggcattcaag gaaggcatgc gcagcctgcg cctgtccggc gcgcagaaga
     5881 tcgccgccgg cctgaccacg atggaggaaa tcctgcgggt gacgccgcag agcgagcagc
     5941 gctgagaggt agaggtaggg tggaaaacga ccgcaggtct tttccaccgc gggtgcacgt
     6001 gcgtggtgga aaatcgctgc gcgagttttc caccctacga agtcacgcct gattcaacgc
     6061 cgccaggatg gcgttgacgc cggcctgggc gcactgctcg tcctgctcgg ccgaggcgcc
     6121 ggatacgccg atggcgccga ccacggcgcc atcgaccttg accggcacgc cgccgccgaa
     6181 cagcaccatg cgcgggcgat gggccaggcc gttgcgcacg ctggcgggag cgccggccag
     6241 ctcggcttcc cagcggctgg tggcgagacc gaagctggcg gcggtatagg ccttgtcggt
     6301 ggcgatgtcg cggctcagcg ccggggcgcc gtccatgtgc gccaggtgga tcagctggcc
     6361 ggcggcatcg acgatcgaca cgctgatgcg cacgcgcagg ctgcgcgctt cggcgagcac
     6421 cgccggcagg gccagcatgg ccagatccag ggacagggag ggttgattgc gtaacatcgg
     6481 acaccttgca ttggggttgt tgtttttagg gtggctgggc aaaccgcaac cggggctcag
     6541 gcgctgtggc cgccctgcgt gccgggatcg atcttgccca ggtcctgtag caggcgcatc
     6601 agcgtgcgga agttctccgt gccgaactgg cgctggatgt ccttgtacag gcgggtcatc
     6661 tcgctgctca tttcctcgaa cagcgcgacg cccttgtcgg tcaggctgac gcacagacgg
     6721 cgccggtcgc tggccggttt ccagcgccgc accagaccgt cgcgctccag gcgcaccagc
     6781 accccggtca ggctcgggcg caggatgcag gcctgctccg ccagctggtg gctctccagc
     6841 tcgtcgcggc gatgctggct gaggatgcgg atgacccgcc actgctgctc ggtcagttcg
     6901 tactgattga gcagcggacg gaagaagccc aacgtcgctt cgcgcgcctg cagcagggcc
     6961 tggatcagtg aggggtgggg cttgtgcatg ggggcctgga tcttcctgcg aaaacgacat
     7021 gtcgccgccc caggcgggac ggcgacgttt taccacagat ggcggcggcg ctcgcggcag
     7081 gtgcggcctc agtagacgct gccgctgacg gacggcgccg actcgatccc cttgaagctg
     7141 ttcgccagac gcttcagcga gcgcatcagc acgctgctgt cgacgcccac ggcgacgaag
     7201 gaacagccca gctccagata gcggcgggcg agtttctcgt ccccggagag aatgccggcc
     7261 gccttgccgg ccttgcggac ccgggcaatg gcgtcctcga tggcggcctg cacctcggga
     7321 tggccggggt tgccgcggtg gcccatggcc gccgacaggt cggccgggcc gatgaacacg
     7381 ccgtcgacgc cctcgacggc ggcgatggcg tcgaggtttt ccagaccctt gagattctcc
     7441 acctggacca gcaggcacat ctgcgcgtcg gtttcatcca ggtaggtggt gatgttgttc
     7501 cagcgcgagg cgcgcgccag ggaggcgccg acgccgcgaa tgcccttcgg cggatagtgc
     7561 atggcggcga ccagttcttc ggcctgctcg gcacgctcca ccatcggcac cagcagggtc
     7621 tgcgcgccga tgtcgagcag ctgcttgatc agcaccggat cgccgatcgg cggccggatc
     7681 accggctggc tggcataggg ggccaccgcc tgcagctggc cgagcagcga gcgcaggtcg
     7741 ttgggcgcgt gttcgccgtc gagcagcagc cagtcgaaac cggcgttggc cgccagctcg
     7801 gcgcagtagg gatcggccag accgatccac aggccgatct gctgctcggt catcaggcgc
     7861 tgcttgaagg tgttgactgg catctgcatc gttctgttct caaacaaggg gcaggagatg
     7921 gggcccggca ggtcgccgtc gaccaaaacg ctggcggacg acgggagcca gtggcgggga
     7981 cgatccacgg ggcatttcct tagggtgcca gcttgcaggc ctgtccggct ataccccagg
     8041 gcaatcgcat gaggctgaaa ggtcaacata cgtggggtaa gtcctctgca ggagcgaatt
     8101 cattcgcgac tgcgctcccg aagtcgcgaa tgaatccgct ccgttcatgc gattgccctg
     8161 ggcttcggat gcttgtgcac tctgcgcccc ggaggatgac caagcagcca ctcgccatga
     8221 cccgcccggt aaactacccg cccgccgtcc gggggagctt gtactgcccc tcggcccgtc
     8281 atctgtctgg agaccgcatg ccccctgtcg ttgccaaccc ccaacgcctg atcgtcctgc
     8341 tggctgcgct ggtggccttc ggcccgctgt cgatcgacat gtacctgccg agcctgccgc
     8401 tgatcgcccg cgacctcggc gcgcccgagg cgcagatcca gctgaccatc agcgtgttcc
     8461 tcgccggcct gtgcaccggc atgctggtct acggcccgct gtccgaccgc ttcggccggc
     8521 gcaagctgct gctcggcggc atcgtcctgt acatggtcgc cagcctcggc tgcatcctcg
     8581 ctgccaaggc cgagcacctg gtgttctggc gcatcctcca ggccctcggc ggcgccggcg
     8641 cctcggtgct ggcgcgcacc atcgtgcgcg acctgttccc gctcaaggac gccgcccgcg
     8701 tgctgtcgct gatgcacctg gtgaccatgt tcgccaccct gatcgccccg ctgatcggcg
     8761 gctacctgat cctgctcgcc ggctggcgct cgctgttcat ggtgctgatg gccttcgccg
     8821 ggctgtgcct gctggccgtc gtctggaaga ttccggaaac ccacccggcc cacgcccgcg
     8881 gcgcctcggt cggcgcggtg ttccgcgcct atgggtatat cgccgtgcag ccgcaggcgc
     8941 tcggctacat cctgtgcatg ggcctgtcgt tcggcggcat gttcgccttc atcaccgcct
     9001 cgccgttcgt ctacatccag tacttcggcg tgtcgccgca gcagtacgcc tggctgttca
     9061 gcctgaacgt cttcggcatc atcgtcgcca ccctgctcaa cgcccgcctg gtcggccgcc
     9121 tcggcccgca gcgcctgctg gtgtgcggtg cgagcatcgc cgcgctgtcc ggcctgggcc
     9181 tggcgatcct cggcagcagt ggcgtcggcg gcctgaccgc catcgtcgtc tgcctgctgt
     9241 tctacgtcag cgtcaccggc ctgctcggcg ccaactgcgt ggccagcctg ctgtcacact
     9301 tccccaaaca ggccggcgcc gccgccggcc tggcggtagc ctcgcagttc ggcctcggca
     9361 ccgccgccag cgcgctggtc agcgccctgc acgacggcag cccgctgccg atgagcctgg
     9421 tggtcggcgc caccggggtc ggcagcctgc tggccctgct gctgacccgg ataccggagc
     9481 gtgcggccgc gtcagctcag ggaaaataac tcgaccactg taattggcat gtcatatgca
     9541 ttaagtaccg tatccagttt cggggaccac ggtacaggca ggccggggat tccccctttt
     9601 atgcccaaag ccccggatcg cagcgcgatc cggggctttt tcatgcctgg tgggtctgtc
     9661 gcccctctcc cgctggcggg agagactctg gagagaggga aagcggactg tcggatagct
     9721 gccagactgt cgcttcccgc acacgaatcg tgcacttgca ccggggaggt gcaggctcag
     9781 cgatagatct cgcggcggaa gatcagcgcc cgctggctgg acaacacgcc gaaggtcgcc
     9841 agcgccgaag gcgcctcggg cgtaccgttg ccatcccaat cgaacagcag ccagggcagt
     9901 gccggggtcg cgccattcag gccggacagg ctggccagca cgctgccttt cttattgggc
     9961 gccggcaggc gaatgacacc ggtcggcgtc ggcgtgctgg ccggcggcgc tgccagcgtg
    10021 ccagggggcg ccgggaaatc gctcgcctgc aggtagccgg tgaagtcgga cagcaccaca
    10081 tcgcccagcg ccgccagcga acagctgtca tccgtcgtcg ctccaaatgc attgccgccg
    10141 gccgaggcct gccagctttc cagccagtag ggcagatcca gcgcctggtt ctccgggcca
    10201 ctggcggcat cgatgcgcag gcggcccagg cgtacttcgg tgccgccgaa agcatggacg
    10261 aagtcctggc aggtggcggc gctgccaagg taacagatgc cgtcgagatc ggtcagttcg
    10321 ctggtcccaa ccctaagctc aacatggtca cccgccgcgg tcagcacgaa cggcaggtcc
    10381 tcggccgtgg gcggcgacgg cagccgccac agcagcgcat cggccaggcg cacgccatcg
    10441 gcccgccagt cgaagacccg cctaccgtcg gcgccaccgc tatccgccac cgccagcgac
    10501 tgtggatcgc ccagactctg caggcgagaa accaggtccg gcttgctggt gtcgctgaag
    10561 ctgtaggcct ggcgctgcgg cgcctccagc cgccagaacg ggtcgcggtc gtagttattg
    10621 gtgaccgccc cctggcggtt gtagccagtg accactatgc ccggcggccc ggcggaaaaa
    10681 ttgatggttt gaccttgata actgaaggcg ccgcaggccg gctccaggct ggcggtgctg
    10741 gtcgcgccca gatacgcggg ggtgaagcgg ccgatcagcg cgctctcgcc gccgctgacc
    10801 gtcagggtgt ggtaggtacc agccggcggc gtggcggtga tgcggtatat accgacctcg
    10861 ctctgaccga cctgcagcgt ggtcgaggcg cccactgcat ggtcgtagct gatgagcgcg
    10921 gcctgccgag tgccatcggc agcatgccgg tagaaggtgc cggccacgcc gccgctcgcg
    10981 acgctcggct cgaccagcat ggagttgagg gcgatgccgt tcagttggaa gttcggtgtg
    11041 gtcgggttgt cgctcagcgc cgcggcggtc agcgcctcgc cgtcagcctg ccaggctacc
    11101 gcgcggatgc tcagcgggaa gctgtcgccg gccgcacgca gcatgctgca acccgacacg
    11161 ctcgccaccg tgcagctggt gtcggccggt gtggagatat gcaggccata gggcttgctg
    11221 acgaactggt cggcgccggt catgaccagg ccgctctcca gccccgcgcc atcgaagcgg
    11281 gcattcagct gcatcaggcc ggcatcgtcg tagcgcacct tcagctgtgt cgtggcctgg
    11341 ttgtcgaaga caagcgacag gcctgtcggt gacgccgcat tgccggatac cgccgtatcg
    11401 ttgacctgca ccgccatgct gcccgtgccg ggatcgacat agtcgctcca gaactgcagc
    11461 gtgcgggtgg ccggaccgaa ggccggcacg cagcgctggg tcgcgttgtc cttgcgtacc
    11521 gcctgcaggg cgatgccggt ctgctccttc ctggccagca ggttcggcac gtcgaagatg
    11581 aagccgctgt cggcgaaggc cacctggcag ttggtcgaca gcggcgccgc cccgacctta
    11641 cacagcgtct gactcaatgc cttcagcggc ggattggagt tgctcacccc cagagtcacg
    11701 gtctgcgccg tggtgacgcg caggttgtcc accagcgtgc cgccactgaa cgtccggctg
    11761 ctgccgccct cccagccact cggcgacagg gtgacgctgg tcggatcggt atgccgcagc
    11821 gagcaatcgg cattcaggca ggcgcgcacc tcgacgctca gcggattgca ggtcagcgca
    11881 ttgccggcat gcacgaactc gtagtggtcg atctgctgac cgatcgcgtt catctgggtc
    11941 gcgcagacct gcagatcgtc cagctcgtgg atattgttcg agccgccggt agaccccgtg
    12001 aacgtcaaaa aaaagttggc cggcagggcg gcctgccccg aactggccag cacgtcgaac
    12061 gaggaaatca gcgtcacgaa gcccgcgccg gtattgcgct cgacctggag catcgacttg
    12121 ccggcaaccg tgttgtccag cgtgatgcga taggtatgcc ccggccccgc ggtactcccg
    12181 gagatatcga tacccgggct gaggttggcc gcggtgcctg ccagatagcg atagcccgtc
    12241 gtgccggagc cggagccgcg gaccgacacc gagtcctgac gaaaccccgg cccgcctacc
    12301 cgcccctcgg tcgggttgga gaagttgccg tactcgtcca gcgccacggc cagccagccg
    12361 ccggcaaaac cgctgatgct gccgttctgg gcataaccga gcgagccgcc atagccccct
    12421 ggctggggcg tttgcgtcgc atcggagaga atcatcgcta ccccatccgc gccgctgccg
    12481 ttataggcgt agtacttgaa ctgtacctgc acgaagttgg cggtagcggg caccaaccgc
    12541 tgaatggtgg cgccagtggc gacgttgccg gagctgtcgg tgagccgcag tcggttgttg
    12601 acgatccgcg gtgcgccgaa actgccattg cggctggtca ccgcccagtc gctggagccc
    12661 agagccaggc gattgaagtc gtcggcgaga caggtctggg gagccggcgg ctcgggtggt
    12721 gcggcataaa tcaccctcgc cccgctggac tcgagagtga catcgtcagc cgttatcgcc
    12781 cccttccaag tagctgcatt gccgatcgtc acctttccct tgctgtagat gtagccgggg
    12841 accagactct gattatccac tgtgaagtcg ttgtatgcat agatgaacaa ctgttcgggg
    12901 agtcccggtg tagcggcgtc ccggtagttc attatcgact taaatgaaac actaaaagac
    12961 tttcgcgcaa atatcctaac cgtctctccg gcggggatga tgatcttgac ttcagtaccg
    13021 ctgaatttta tttcatcaat ccagtagtct ccccctccca agaccaatgt tgcacgatat
    13081 gccaaatcca gcttatctat aaaaaaggtc tcgccattgc cattgacggt cagcgtggcc
    13141 tcctgcccaa catcccaatt tttatactca ttgctcccat tggcgtttac agctgcactt
    13201 cccctataac caacattgac tgaagggagc gggcccgaag cactccgaaa actgccagca
    13261 tcctgtttcg gtgcagccgg tgatatcgct tggcagaacc cggaggcacc gcaggttggc
    13321 gtaggctgag ataaattctg cgcgatactg ccggcccgaa cttgctgatt gtcggtttcc
    13381 agtacgccat tccagccaaa ggtaaccttg ccattggtcg tgtgactctg aatgccattg
    13441 gggaaattat cggcattaat tccgagacca gcccatgccg aaaccaccca ccccagcatg
    13501 agtacgctta acaaaattcg gcacatagac tatttctcca ccatcacttc caaccgtcgc
    13561 caggcatagt ccacccggct gtccgggctg ccgttgtagg ccgtagcggt cagacggtac
    13621 agattgaagg tgcggatacc ctccgtgtag gtctgcgcgc aggtggccgc ggcacagggc
    13681 agcttgacgc ag
//
LOCUS       DATCUN010000113         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_117549, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000113 DATCUN010000000
VERSION     DATCUN010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117549"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VIO83_04395"
     CDS             <1..279
                     /locus_tag="VIO83_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY8330549.1"
                     /translation="AGGRIWLAPGMLHAKLAVVDDALALAGSANLDSRSLFLNYELML
                     AFHQGADVRRFAAWFDREITTASPYVATPPSLLRDAGEGLLQLLGFQL"
     gene            complement(391..999)
                     /locus_tag="VIO83_04400"
     CDS             complement(391..999)
                     /locus_tag="VIO83_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY8330550.1"
                     /translation="MFEGIVDFIAETGYLGVFLLMVLENLFPPIPSELIMPLAGFAAA
                     QGELSIPGVLLAGTAGSVLGALPWYYAGYFFGSARLKMLAGRFGRVMTLSPRDVDTAC
                     VWFERHGWQATLFGRLVPAVRTLISAPAGMVAMPMRAFLFYSTLGSLVWTSILTVAGY
                     LLHGQYHLVADYVDPLSKVVVLVVVGVYLYRLITHKGTSAEA"
     gene            complement(1118..2050)
                     /locus_tag="VIO83_04405"
     CDS             complement(1118..2050)
                     /locus_tag="VIO83_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8330551.1"
                     /translation="MNETFDPQRLRQQLPPLSAAAAESGPQVQAYRRYYGLQHRPDIH
                     SRLGWVEVEGRKLAVQGWWRDDARATILLLHGYYDHMGLYRHVFDWALAMNFAVLSCD
                     LPGHGLSEGAPASIGDFAEYQAVLAALFQAAELLHLPQPWHLFGQSTGGAILLDYLLT
                     GAPRPDLGQTVLLAPLVRPYAWTRGKLSYHLLNPFVSQIARRYSDNSGDTAFLDFVRN
                     DPLQAQILPTAWVGALARWIPHIENAPRSGHSPLIIQGDADTTVDWQHNLGVLQDKFR
                     GAEQLILSGARHHLVNEEEVQRRRLFELLGERFA"
     gene            2162..>2718
                     /locus_tag="VIO83_04410"
     CDS             2162..>2718
                     /locus_tag="VIO83_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016782724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6436 domain-containing protein"
                     /protein_id="HEY8330552.1"
                     /translation="MPLPRRKTVITSLLVLVAAAALWSLYQWYQARYIRPFSETTVLF
                     DAAGLRLPAGLAGPGAIRVVHLWDPPCPCNVGNQQHLAELVQRFAGQGVSFHVWQKPG
                     SRGQLPDTLKALQPLAELPGAERLPASPAVAIWDRDGKLAYVGPYSEGATCTSSNSFI
                     EPVLEALLAGRPVHSTGSLAVGCYCP"
BASE COUNT          430 a          921 c          971 g          396 t
ORIGIN      
        1 gccggtgggc gcatctggct ggcgccgggg atgctgcacg ccaagctggc ggtggtcgac
       61 gatgcgctgg cgctggccgg ctccgccaac ctcgacagcc gcagcctgtt cctcaactac
      121 gagctgatgc tggcgttcca ccagggcgcc gacgtgcgcc gcttcgccgc ctggttcgac
      181 cgggaaataa ccacggccag cccgtacgtg gccacgccgc cgagcctgct ccgcgatgcg
      241 ggcgagggcc tgctgcagtt gctgggcttc cagctgtgag ccgggagcga cgggcctgcc
      301 gcgtccgggc cccagaccac ctgcggtcgg acgccagaac gcaaaagccc gaggcgatgc
      361 ctcgggctgc gtgaccgacg gtagcgctgc tcaggcctcg gcgctggtgc ccttgtgggt
      421 gatcaggcgg tacaggtaca cgccgaccac caccagcacc accaccttgg acagcgggtc
      481 gacgtagtcg gcgaccagat ggtactggcc gtgcagcagg tagccggcga cggtcaggat
      541 ggaggtccac accagcgagc cgagggtcga atagaacagg aaggcgcgca tcggcatggc
      601 gaccatgccg gcaggcgccg agatcagcgt gcgcaccgcc gggaccaggc ggccgaacag
      661 ggtggcctgc cagccatggc gctcgaacca gacgcaggcg gtatccacat cccgcggcga
      721 gagggtcatc acccggccaa agcggccggc cagcatcttc agtcgcgcgc tgccgaagaa
      781 atagccggcg tagtaccagg gcagtgcgcc caacaccgag ccggcagtcc ccgccagcag
      841 cacgccggga atggacagct cgccttgggc ggcagcaaag ccggccagcg gcatgatcag
      901 ctcggagggg atgggcggga aaaggttttc cagcaccatc agcaggaaca cgccgagata
      961 gcccgtctcg gcgatgaaat cgacaatgcc ttcaaacatt cgtggtggtc tctcgcgcca
     1021 gtgcgcctgg gttggtctgg gcagttttct gccagggtgg taactgcacc catgataaca
     1081 gcggcgctgc ccggaaggct gccgccggct acagcgttca ggcgaagcgt tcgcccagca
     1141 gttcgaacag ccgccggcgc tgcacctctt cctcgttgac cagatgatgg cgcgcgccgg
     1201 acaggatcag ctgttcggcg ccgcggaact tgtcctgcag cacgccgagg ttgtgctgcc
     1261 agtccaccgt ggtgtcggcg tcgccctgga tgatcagcgg gctgtggccc gagcgcgggg
     1321 cgttctcgat atgcgggatc cagcgcgcca gcgcgccgac ccaggcggtg ggcaggatct
     1381 gcgcctgcag ggggtcgttg cggacgaagt cgaggaaggc ggtatcgccg gagttgtcgc
     1441 tgtagcgccg ggcgatctgg ctgacgaagg ggttgagcag gtggtagctg agcttgcccc
     1501 gcgtccaggc gtacgggcgc accagcgggg cgagcagcac ggtctggccg aggtccgggc
     1561 gcggcgcgcc ggtcagcagg tagtcgagca ggatggcgcc gccggtgctc tggccgaaca
     1621 ggtgccaggg ttgcggcagg tgcagcagct cggcggcctg gaacagcgcg gccagcacgg
     1681 cctggtattc ggcgaagtcg ccgatgctcg ccggcgcccc ctcggacagg ccgtggccgg
     1741 gcaggtcgca ggacagcacg gcgaagttca tcgccagcgc ccagtcgaac acgtggcggt
     1801 acaggcccat gtggtcgtag tagccgtgca gcagcaggat ggtcgcccgc gcgtcgtcgc
     1861 gccaccagcc ttgcacggcc agcttgcggc cctcgacctc gacccagccg aggcggctgt
     1921 ggatgtcggg acggtgctgc aggccgtagt agcgccggta ggcctgcacc tgcgggccgc
     1981 tctccgccgc cgcggcggac agtggcggca gttgctggcg cagacgctgg ggatcgaagg
     2041 tttcgttcat gggctcggcc gcgcggctgg cggactgtgc tgatggatgg ctaatgtcga
     2101 tcttcagacc gggcgcgcga catggcaagc ttggccgtca ttttccgccg acgcgcccgc
     2161 catgcccctg cctcgccgca agaccgtcat caccagcctg ctcgtcctgg tcgccgccgc
     2221 cgcgctgtgg agcctgtacc agtggtacca ggcccgctat atccgcccgt tcagcgagac
     2281 caccgtgctg ttcgacgccg ccggcctgcg cctgcccgcc ggactggccg ggccgggcgc
     2341 gatccgcgtg gtgcacctgt gggacccgcc ctgcccgtgc aacgtcggca accagcaaca
     2401 cctcgccgaa ctggtgcagc gcttcgccgg ccagggcgtg agcttccacg tctggcagaa
     2461 acccggcagc cgcggccagt tgccggacac cctcaaggcc ctgcagccgt tggccgagct
     2521 gcccggcgcg gaacgcctgc cggccagccc ggcggtggca atctgggatc gcgacggcaa
     2581 gctggcctac gtcggcccct acagcgaagg cgccacctgc acctcgagca acagcttcat
     2641 cgaaccggtg ctcgaagcgc tgctcgccgg gcggccagtg cacagcaccg gcagtctggc
     2701 ggtgggctgc tattgtcc
//
LOCUS       DATCUN010000114        15882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_98961, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000114 DATCUN010000000
VERSION     DATCUN010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 15882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15882
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98961"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1082
                     /gene="dmpG"
                     /locus_tag="VIO83_04415"
     CDS             51..1082
                     /gene="dmpG"
                     /locus_tag="VIO83_04415"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HEY8330553.1"
                     /translation="MTFDPSKKLYISDVTLRDGSHAVRHQYSIQNVQDIARALDEARV
                     DSIEVTHGDGLQGSSFNYGFGAHTDLEWIEAAADVIQHAKITVLLLPGIGTVHDLKAA
                     YDAGARSVRIATHCTEADVSKQHIEYARSLGMDTVGFLMMSHMIPAEQLAAQGKLMES
                     YGAQCIYMADSGGAMNMQDIRDRMRAFKAVLNAETQTGMHAHHNLSLGVANSIAAVEE
                     GCDRIDASLAGMGAGAGNAPLEVFIAAAERMGWNHGTDLYRLMDAADDLVRPLQDRPV
                     RVDRETLGLGYAGVYSSFLRHAEVAAAKYGLKTLDILVELGRRRMVGGQEDMIVDVAL
                     DLLAAGKGH"
     gene            1170..2417
                     /gene="mhpT"
                     /locus_tag="VIO83_04420"
     CDS             1170..2417
                     /gene="mhpT"
                     /locus_tag="VIO83_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HEY8330554.1"
                     /translation="MKTCTAQAFSQAPAELQQDSRRTWLTIALCFAVAILEGIDFQAP
                     GIAAKGMGVAFGLDKLQMGWVFSAGILGLLPGALVGGWLADRIGRKRVLIASVALFGI
                     FSIATAHAWELYSLMLARLLTGVGLGAALPNLIALCSEAAGPRLRGTAVSLMYCGVPI
                     GAALAAMIGIVGFFDDWTVPFYVGGVLPLLVLPLLLLWLPESAAFRARQASASEEPHV
                     PVIDGLFRNGAALPTMLLWVAYFFTLMVVYMLISWLPSLLVGQGFSGVQASWVVFTLQ
                     IGAAVGTLMLGVLMNTLRPLAIAGLIYGGLLAALAALGIVESFVGMLFAGFAAGVFAT
                     GGQGVLYALAPLFYRTEVRATGVGSAVAVGRLGAMSGPLVAGKMLALGTGTAGVMIAS
                     APGLVVAGIAVFYLFVRSRKQSV"
     gene            2717..4069
                     /locus_tag="VIO83_04425"
     CDS             2717..4069
                     /locus_tag="VIO83_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009401460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015802 - basic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family porin"
                     /protein_id="HEY8330555.1"
                     /translation="MHFKRTLIASAILCGQVGLAQAGGYVETLPAAAGWPSGKVARAQ
                     AGGFVEDSKATLTLRNYYFDRDFRSNNPNLPARINDVSQSQAWAQGLILRMESGFTEG
                     PVGFGLNVLGTAGFKLDGGPGRSLLGNDLLPSDPQTNEPGDNYSELGLTAKAKISASE
                     LQVGTLSPTLPVILSAPTRLFTPTFRGAYLRSDEIDNLTLHLGHIDRMNVRNSSNYEP
                     LRVNGPYGRFNEAAESNSFDFAGGDYRWSDSLVTSYNYAQLKEIYQQHYFGLVHTLPL
                     GEGSKLKTDLRYFNSREDGEARAGKVDNQSFGLRFNWLTGPHSLMVGYMQQSGASAQP
                     FIYRADVHVHSELAMTSDFVNPDERTWGARYDYDFVALGVPGLRGMLRYIHGDNIELP
                     GVDHRASEIERGVELAYVVQSGSLRGLGLRWRYASNRNDYFRDVDETRINIDYTLALW
                     "
     gene            complement(4112..5731)
                     /gene="glpD"
                     /locus_tag="VIO83_04430"
     CDS             complement(4112..5731)
                     /gene="glpD"
                     /locus_tag="VIO83_04430"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEY8330556.1"
                     /translation="MTASQPISAPLAEVYDLAVIGGGINGCGIAADAAGRGLSVFLCE
                     QHDLAAHTSSASSKLIHGGLRYLEHHEFRLVREALGEREVLLNMAPHLVRPLRFVLPH
                     RPHLRPIWMIRAGLFLYDHLGVRHSLPGSRHLTFGPGCPLKAEIAEGFEYSDCWVDDA
                     RLVVANAISAREHGAHIHPRTRCIGARRSKGLWHLHLEREDGSLLSLRARALVNAAGP
                     WVEQFLQETLHQPSAQHLRLIQGSHIVVPRLHDGEQAYILQNEDGRIVFVLPYQQRFS
                     LIGTTDCEFHGDPATVQISDEEIDYLLTVVNTHFRQQISRSSIVNHFSGVRPLYDDAS
                     GSPSAVTRDYHLALASEHDGAPLLSVFGGKLTTYRKLAEAALEQLAPLFPHLQPAWTA
                     SSKLPGAEELDSVSKLADELCDSHGWLSPDLARRWAGSYGTRAWQLLEDVHCLMDMGE
                     NFGAGLYAREVDYLCSEEWARSAEDILWRRSKLGLFLGPMQQQRLVRYLQAQHGEVTE
                     QARPIVHERLAPAYATPLLRLDDSATKRPAR"
     gene            5866..6336
                     /gene="ybaK"
                     /locus_tag="VIO83_04435"
     CDS             5866..6336
                     /gene="ybaK"
                     /locus_tag="VIO83_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEY8330557.1"
                     /translation="MTPAIDLLKKVRAVHTVHSYSHDPKAPSYGLEAAEKLGLEPARV
                     FKTLLAASEKGELLVAVVPVAGTLDLKALAQAAGVKKADMADPAAAQRATGYLLGGIS
                     PLGQKKRLRTFIDASAQAFPTVFVSAGRRGLEVELVADVLAQHCQASFAAIGRE"
     gene            complement(6395..7483)
                     /locus_tag="VIO83_04440"
     CDS             complement(6395..7483)
                     /locus_tag="VIO83_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013973961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8330558.1"
                     /translation="MPNRLLLNLRNLVCGYREQRVVQNVSLHLNAGDIGCLLGPSGCG
                     KTTTLRAIAGFEPVQEGSIELAGEVISRPGFTLAPEKRRIGMVFQDYALFPHLTVAEN
                     IAFGIRQHPQRERIAAEMLELVNLGALGQRYPHELSGGQQQRVALARALAPEPRLLLL
                     DEPFSNLDVELRRRLSHEVREILKARGTSAILVTHDQEEAFAVSDQVGVFKDGHLEQW
                     DTPFNLYHEPLTPFVASFIGQGYFVRGQMLSHDTVQTELGVIRGNRAYQWAPGTAVDM
                     LLRPDDIVHAPDSALQARIVGKSFLGAATLYRLQLATGTHLEAVFPSHADHLVGEDIG
                     IRIDADHLVAFPAQGSVAAQLSRTEPRA"
     gene            complement(7498..8415)
                     /gene="argF"
                     /locus_tag="VIO83_04445"
     CDS             complement(7498..8415)
                     /gene="argF"
                     /locus_tag="VIO83_04445"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY8330559.1"
                     /translation="MSARHFLSLMDCTPQELASLILRGIELKDLRNRRVLYEPLKNRV
                     LGMIFEKASTRTRVSFEAGMIQLGGQAIFLSPRDTQLGRGEPISDSAIVMSSMLDAIM
                     IRTFAHQKVLDFAAHSRVPVINGLSDDLHPCQLLADMQTFHEHRGSIQGKTVAWVGDG
                     NNMCNTYAQAAVQYDFQLKVACPEGYDPKREFIEQAQGRVTLVRDPREAVAGAHLVST
                     DVWASMGQEEELAARMQLFAPYQVSRALLDQAASDVLFMHCLPAHRGEEISLDLLDDP
                     RAVAWDQAENRLHAQKALLEFLVEHAGNV"
     gene            8691..9014
                     /gene="grxD"
                     /locus_tag="VIO83_04450"
     CDS             8691..9014
                     /gene="grxD"
                     /locus_tag="VIO83_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003295894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEY8330560.1"
                     /translation="MDIIETIKEQIASNPVLLYMKGSPNAPQCGFSARASQAVMACGE
                     KFAYVDILQHPEIRASLPQYANWPTFPQLWVDGELVGGSDIILEMHEKGELQPLIKAA
                     VHKGE"
     gene            complement(9077..9547)
                     /gene="bfr"
                     /locus_tag="VIO83_04455"
     CDS             complement(9077..9547)
                     /gene="bfr"
                     /locus_tag="VIO83_04455"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEY8330561.1"
                     /translation="MKGDKKVIQHLNTILGNELVAINQYFLHARMYEDWGLKRLGKHE
                     YDESIDEMKHADRLIKRILFLEGLPNLQDLGKLLIGEHTKEMLECDLKIEQKAVPDLK
                     AAIAYCESIADYGTRDLLEEILDSEEEHIDWLETQLDLIGKVGLENYLQSQMHD"
     gene            complement(9677..9895)
                     /locus_tag="VIO83_04460"
     CDS             complement(9677..9895)
                     /locus_tag="VIO83_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEY8330562.1"
                     /translation="MYVCLCLGVTDHQIRDAIHEGCCSYRQVRESTGVGTQCGKCACE
                     AKKLVRHTIHDVHQAQIALAHPAAFAAA"
     gene            complement(10065..10742)
                     /gene="rnt"
                     /locus_tag="VIO83_04465"
     CDS             complement(10065..10742)
                     /gene="rnt"
                     /locus_tag="VIO83_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEY8330563.1"
                     /translation="MSEQIDYEDYEQEPNTSGFKPPMARRFRGFLPVVVDVETGGFNC
                     ATDALLEIAATTIAMDDDGLLYPEHTAFFRIEPFEGANIEQAALDFTGIKLGHPLRMA
                     VSEEQALGEIFKSVRKSLKSSGCKRAVLVGHNSSFDLGFLNAAVNRTGIKRNPFHPFS
                     SFDTATLAGLAYGQTVLAKACQAAEIPFDNREAHNARYDTEKTAELFCGIVNRWKEMG
                     GWIDFDE"
     gene            complement(10739..11785)
                     /gene="pyrC"
                     /locus_tag="VIO83_04470"
     CDS             complement(10739..11785)
                     /gene="pyrC"
                     /locus_tag="VIO83_04470"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY8330564.1"
                     /translation="MSDRLTLLRPDDWHIHLRDGAALAHTVPDAARTFGRAIIMPNLV
                     PPVRNAEQAAAYRERILAARPAGSRFEPLMVLYLTDKTSPEEIRAAKAGGLVHAAKLY
                     PAGATTNSDSGVTAVDNIFAVLETMAEVGLPLLVHGEVTHADIDVFDREKRFIDENLR
                     RVVERFPTLKVVFEHITTGDAAQFVREAPANVGATITAHHLLYNRNHMLVGGIRPHFY
                     CLPILKRNTHQEALLDAATSGNPKFFLGTDSAPHARHVKEAACGCAGCYTAFAAIELY
                     AEAFDRRGALDKLEGFASQFGPDFYGLARNSDTITLIREDWVAPTELHFGDHQLVPLR
                     AGEKLHWRVLENEA"
     gene            11957..13174
                     /locus_tag="VIO83_04475"
     CDS             11957..13174
                     /locus_tag="VIO83_04475"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY8330565.1"
                     /translation="MADVKKVVLAYSGGLDTSVILKWLQDTYNCEVVTFTADLGQGEE
                     VEPARAKAQAMGVKEIYIDDLREEFVRDFVFPMFRANTVYEGEYLLGTSIARPLIAKR
                     LIEIANETGADAISHGATGKGNDQVRFELGAYALKPGVKVIAPWREWDLLSREKLMDY
                     AEKHAIPIERHGKKKSPYSMDANLLHISYEGGVLEDTWTEHEEDMWRWTKSPEAAPNV
                     PTYIELTYRNGDIVAIDGVEMTPATVLTKLNQIGGENGIGRLDIVENRYVGMKSRGCY
                     ETPGGTIMLRAHRAIESITLDREVAHLKDELMPKYASLIYNGYWWSPERQMLQQMIDA
                     SQATVNGVVRLKLYKGNVIVVGRKSDDSLFDANIATFEDDAGAYNQADAGGFIKLNAL
                     RLRIAAGKGRSPL"
     gene            13322..13714
                     /gene="gloA"
                     /locus_tag="VIO83_04480"
     CDS             13322..13714
                     /gene="gloA"
                     /locus_tag="VIO83_04480"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEY8330566.1"
                     /translation="MRLLHTMLRVGDMDKSIAFYTEVLGMTLLRRKDYPDGQFTLAFV
                     GYGNEADNSVIELTYNWGVDSYELGTGYGHIALEVEDVYKACEDIRARGGKITREPGP
                     MKHGSSILAFVEDPDGYKIELLSPHRGD"
     gene            complement(13770..14180)
                     /gene="uraH"
                     /locus_tag="VIO83_04485"
     CDS             complement(13770..14180)
                     /gene="uraH"
                     /locus_tag="VIO83_04485"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEY8330567.1"
                     /translation="MNALKSLFVGALLSGLSSLALAAGNPLSVHVLNLEDGLPSPDVT
                     VTLEKQDGAQWTELNSAVTNPQGRITALFPEGQALEKGTYRVTFKTGEWFAKQQAATF
                     FPEVPVIFTADGSVPHYHIPLLLSPYGYSTYRGN"
     gene            complement(14194..14694)
                     /locus_tag="VIO83_04490"
     CDS             complement(14194..14694)
                     /locus_tag="VIO83_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY8330568.1"
                     /translation="MPIRTARLSLPGLALCAANAFAAPAQVEQRSDVSLALANQLLDA
                     TLAACHADGRSAVAAVVDRSGNLVAVQRDDNVGPHNTLAAQRKAYTALSTKTATRLLA
                     ERARTNPEAANLNTLDELLLLGGGVPLQVGGEVIGAIGVAGAGGAAIDEACAIKAIAQ
                     LLPQTH"
     gene            14844..15515
                     /locus_tag="VIO83_04495"
     CDS             14844..15515
                     /locus_tag="VIO83_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017479552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HEY8330569.1"
                     /translation="MRILVVEDELKTADYLRKGLGESGYLVEVALNGLDGLHLIQESE
                     FDLIILDVMLPGLDGWQLLQVIRRRLPTPVLFLTARDAVEDRVKGLELGADDYLVKPF
                     SYAELLARVRTLLRRGPPREVEQFRVADLELDLLRRRVTRAGERVNLTNKEFALLHLL
                     LSREGEVLSRAYIASQVWQMNFDSDTNVVDVAIRRLRAKIDDPYPCKLIHTVRGMGYV
                     LEVQP"
     gene            15512..>15882
                     /locus_tag="VIO83_04500"
     CDS             15512..>15882
                     /locus_tag="VIO83_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEY8330570.1"
                     /translation="MSLWPRALSLRLALLFALVGVLLLGAIGFYLYQSLEREIAWRDD
                     QALLGRLERMRTLLDDSASIEALRQRPQLYANMLGNRDSLLWIVSDDGQVLIEINPAA
                     LAVPQLPRGPAVQLRDTLVAEP"
BASE COUNT         2573 a         5052 c         5499 g         2758 t
ORIGIN      
        1 accgccgagc gcatggctct ttccatgatc aacggctgag gagcactgcc atgaccttcg
       61 acccgagcaa gaagctgtac atctccgacg tcaccctgcg cgacggcagc cacgccgtgc
      121 gccaccagta ctcgatccag aacgtgcagg acatcgcccg cgccctggat gaggcccgcg
      181 tcgattccat cgaagtcacc cacggtgacg gcctgcaggg ctccagcttc aactacggct
      241 tcggcgccca caccgacctg gagtggatcg aggccgccgc cgacgtgatc cagcacgcca
      301 agatcaccgt gctgctgctg cccggtatcg gcaccgtgca cgatctgaag gccgcctatg
      361 acgccggcgc ccgttcggtg cgcatcgcca cccactgcac cgaagcggac gtgtcgaagc
      421 agcacatcga gtacgcgcgc agcctcggca tggacaccgt cggcttcctg atgatgagcc
      481 acatgatccc ggccgagcag ctggcggcgc agggcaagct gatggagagc tacggcgcgc
      541 agtgcatcta catggccgac tccggcggcg cgatgaacat gcaggacatc cgcgaccgga
      601 tgcgcgcgtt caaggcggtg ctgaatgccg aaacccagac cggcatgcac gcccaccaca
      661 acctcagcct cggcgtggcc aactcgatcg ccgcggtgga ggagggttgc gatcgcatcg
      721 atgccagcct ggccggcatg ggcgccggcg ccggtaatgc tcctctcgag gtgttcatcg
      781 ccgccgccga gcgcatgggc tggaaccacg gcaccgacct gtatcgcctg atggacgccg
      841 ccgacgacct ggtgcgtccg ctgcaggacc gtccggtgcg cgtcgaccgc gaaaccctcg
      901 gcctcggcta tgccggcgtc tactcgagct tcctgcgtca cgccgaagtg gcggcggcca
      961 agtacggcct gaagaccctc gacatcctcg tcgagctggg tcggcggcgc atggtcggcg
     1021 gccaggaaga catgatcgtc gacgtggcgc tggacctgct ggccgcgggc aagggccact
     1081 gatccgctcc tgataaacag cgaccagcta cctgagggta actggtcgtt ttttgtgcac
     1141 tctaataaga aaagaatagg tagaccgaca tgaaaacctg cactgcgcaa gcattttccc
     1201 aagcccctgc cgaactccaa caggacagcc gtcgtacctg gctgaccatc gccctgtgct
     1261 ttgccgtcgc catcctcgaa ggcatcgact tccaggcgcc gggcatcgcc gccaagggca
     1321 tgggcgtcgc cttcggcctg gacaaactgc agatgggctg ggtgttcagc gccggcatcc
     1381 tcggcctgct gcccggcgcg ctggtcggtg gctggctggc cgaccgcatc ggccgcaagc
     1441 gtgtgctgat cgcctcggtc gcgctgttcg gcatcttctc catcgccacc gctcacgcct
     1501 gggagctgta cagcctgatg ctggcgcgcc tgctgaccgg cgtcggcctc ggcgccgcgc
     1561 tgcccaacct gatcgccctg tgctcggaag ccgccggtcc gcgcttgcgc ggcaccgcgg
     1621 tgagcctgat gtattgcggc gtgccgattg gcgcggcgct ggcggcgatg atcggcatcg
     1681 tcggcttctt cgacgactgg accgtgccgt tctacgtcgg cggcgtgctg ccgctgctgg
     1741 tcctgccgct gctgctcctg tggctgccgg agtccgcggc cttccgcgcc cgccaggcat
     1801 cggccagcga ggaaccgcac gtgccggtga tcgacggcct gttccgcaac ggcgcggcgc
     1861 tgccgactat gctgctgtgg gtcgcctact tcttcaccct gatggtggtg tacatgctga
     1921 tcagctggct gccgagcctg ctggtcggcc agggcttcag tggcgtgcag gccagctggg
     1981 tggtgttcac cctgcagatc ggcgccgccg tcggcaccct gatgctcggc gtgctgatga
     2041 acactctgcg cccgctggcg atcgccgggc tgatctacgg cggcctgctc gccgcgctgg
     2101 ccgccctcgg catcgtcgag agcttcgtcg gcatgctgtt cgccggcttc gccgccggcg
     2161 tgttcgccac gggcgggcag ggcgtgctgt atgccctggc accgctgttc tatcgcaccg
     2221 aagtgcgcgc caccggcgtc ggcagcgcgg tggcggtcgg tcgcctgggc gccatgagcg
     2281 gcccgctggt ggccggcaag atgctggcgc tgggcaccgg caccgccggg gtgatgatcg
     2341 cctcggcccc gggcctggtg gtggccggca tcgcggtgtt ctacctgttc gtgcgttcgc
     2401 gcaagcagtc cgtctgagcc tgctccggcg tcgcccgctg gcgatgccgg agccgttccg
     2461 gcacgccgac ttgcaggccg ccgcaggggc gcacccccgc aacaatctgg ctgtgcggtg
     2521 agcgtgccac ccggatcaag cgctaccaac tgatctcacc cccgcgcgag cggcgggtgc
     2581 tgatgctctg cgctccgctt tgacccatac cgctgttccc ctcgactccg gtgaccgggg
     2641 ggagggggca atgactgtcg aaaaagcctg cgctcggtgc aggccgtagt gaatacaaca
     2701 aaaaaggagc aaccccatgc acttcaagcg caccctcatc gccagcgcca tcctttgtgg
     2761 ccaggtggga ctggcccaag cgggcggcta cgtcgagacc ctacctgcgg ctgcgggttg
     2821 gccgtcagga aaagtggcgc gggcccaagc gggcggcttc gtcgaagaca gcaaggccac
     2881 cctgaccctg cgcaactact atttcgaccg cgatttccgc tccaacaacc cgaacctccc
     2941 cgccaggatc aacgacgtat cgcagagcca ggcctgggcg cagggcctga ttctgcgcat
     3001 ggagtccggc tttaccgagg gcccggtagg cttcggcctc aacgtgctcg gcaccgccgg
     3061 cttcaagctc gacggcggcc cgggccgctc cttgctcggt aacgatctgc tgccgtccga
     3121 tccgcagacc aatgagccgg gcgacaacta ctccgagctg ggcctgaccg ccaaggccaa
     3181 gatttccgcc agcgagctgc aggtcggcac cctcagcccg actctgccgg tgatcctgtc
     3241 ggcgccgacc cgcctgttca cgccgacctt ccgtggcgcc tacctgcgct cggatgagat
     3301 cgacaacctg accttgcatc tcggtcacat cgaccgcatg aatgtccgca actcgagcaa
     3361 ctacgagccg ctgcgggtca atggcccgta cgggcgcttc aatgaagcag ccgagtcgaa
     3421 cagcttcgac ttcgctggcg gcgactatcg ctggtcggac agcctggtca ccagctacaa
     3481 ctatgcccag ctgaaggaaa tctaccagca gcactacttc ggcctggttc acaccctgcc
     3541 actgggtgag ggcagcaagc tgaaaaccga cctgcgctac ttcaacagcc gcgaggatgg
     3601 cgaagcgcga gccggcaagg tggacaacca gagcttcggc ctgcggttca actggctgac
     3661 tgggccgcac tcgctgatgg tcggctacat gcagcaaagc ggcgccagcg cgcaaccctt
     3721 tatctatcgc gccgacgtgc acgtgcacag cgagctggca atgacctccg acttcgtcaa
     3781 tcccgacgag cggacatggg gcgcacgcta cgactacgac tttgtcgcgc tcggtgtccc
     3841 ggggctgcgc ggcatgctgc gctacatcca cggcgacaac atcgaactgc cgggcgtcga
     3901 tcaccgtgcc agcgagatcg agcgtggcgt cgagctggcc tatgtggtgc agtccggcag
     3961 cctgcggggc ctcggcctgc gttggcgcta cgccagcaat cgcaacgact atttccgcga
     4021 tgtcgacgag acccggatca acatcgacta caccctggcc ctctggtaag acggcgcggc
     4081 tggcgtgcgg gccgccgggg cccggacgcc ctcagcgcgc cggtcgcttg gtcgcgctgt
     4141 cgtcgaggcg caacagcggc gtggcatagg ccggggccag tcgctcgtgc acgatcggtc
     4201 tggcctgctc ggtcacttcg ccgtgctgtg cctgcaggta gcgcaccagc cgctgctgtt
     4261 gcatggggcc gaggaacagg ccgagcttgc tgcggcgcca gaggatgtcc tcggcgctgc
     4321 gggcccattc ctcgctgcac aggtagtcca cttcgcgcgc gtacaggccg gcgccgaagt
     4381 tctcgcccat gtccatcagg caatgcacgt cctccagcag ttgccaggca cgggtgccgt
     4441 agctgccggc ccagcgccgg gccagatccg gcgacagcca gccgtggctg tcgcacagct
     4501 catcggccag tttgctcacc gagtccagct cttcggcgcc gggcagcttg ctgctcgccg
     4561 tccaggccgg ctgcaggtgc gggaagagcg gtgccagctg ttccagtgcc gcttcggcca
     4621 gcttgcgata ggtggtcagc ttgccgccga ataccgacag cagtggcgcg ccgtcgtgct
     4681 cgctggccag cgccaggtga tagtcgcggg tgacggccga ggggctgccg gaggcatcgt
     4741 cgtacagcgg gcgcaccccg gagaagtggt tgacgatgct gctgcggctg atctgctggc
     4801 ggaagtgggt gttgaccacc gtcagcaggt agtcgatctc ttcgtcactg atctgcacgg
     4861 tcgccggatc gccgtggaat tcgcaatcgg tggtgccgat caggctgaag cgctgctggt
     4921 agggcagcac gaagacgatg cgcccgtcct cgttctgcag gatgtaggcc tgctcgccgt
     4981 catgcaggcg cggcaccacg atgtggctgc cctggatcag gcgcagatgc tgggcgctcg
     5041 gctggtgcag ggtctcctgg aggaactgtt cgacccaggg gccggcggcg ttgaccagcg
     5101 cgcgggcgcg cagcgacagc aggctgccgt cctcgcgctc cagatgcaga tgccacaggc
     5161 ccttgctgcg ccgcgcgccg atgcagcggg tgcgcgggtg gatgtgcgcg ccatgctcgc
     5221 gggcgctgat ggcattggcc accaccaggc gggcatcatc gacccagcag tcggagtact
     5281 cgaagccttc ggcgatctcg gccttgagcg ggcagccggg gccgaaggtc aggtggcgcg
     5341 agcctggcag actgtggcgc acgccgaggt gatcgtagag gaacaatccg gcgcggatca
     5401 tccagatcgg gcgcaggtgc gggcggtgcg ggaggacgaa acgcagcggg cgcaccagat
     5461 gcggcgccat gttcagcagc acctcgcgct cgccgagggc ttcgcgcacc aggcgaaatt
     5521 cgtggtgctc caggtaacgc agaccgccat ggatcagctt gctgctggcc gaggaggtgt
     5581 gcgcggcgag gtcgtgctgt tcgcagagga ataccgacag gccgcggccg gcggcgtcgg
     5641 cggcgatgcc gcaaccgttg atgccgccac caatcacggc gaggtcgtag acctcggcca
     5701 gcggtgcgga gataggctgg gatgctgtca tcatggctga gcctttagtc ttgttgtctt
     5761 gtccatggga caggaccggc tgggagggct gcgcggtcct tgctgcccca cgtttggctt
     5821 ttgcgttacc ttctattcat tctggtccgt tttcgtgaag ccaacatgac ccccgccata
     5881 gatctgctga agaaagttcg agccgttcac accgtgcaca gttacagcca tgaccccaag
     5941 gcgccgtcct acggcctgga ggctgcggag aagctggggc tggagccggc acgcgtgttc
     6001 aagacgctgc tcgccgccag cgagaagggc gagctgctgg tggcggtggt gccggtggcc
     6061 ggcaccctcg acctcaaggc gctggcccag gcggcggggg tgaagaaggc ggacatggcc
     6121 gatccggcgg cggcgcagcg tgccaccggc tatctgctcg gcggcatcag tccgctgggg
     6181 cagaagaagc gcctgcgcac cttcatcgat gcctcggcgc aggcgttccc gactgtcttc
     6241 gtcagcgccg gtcggcgcgg gctggaggtg gaactggtgg ctgacgtgct ggcgcagcat
     6301 tgccaggcca gcttcgccgc catcggccgc gagtagggtt gaccggccgc agcctggact
     6361 ggcgaggctg cggagcggct ccgcggcagg gggatcaggc gcggggttcg gtgcgcgaca
     6421 gctgggcggc cacgctgcct tgggcgggga aggcgaccag atggtcggcg tcgatgcgga
     6481 tgccgatgtc ctcgccgacc agatgatcgg catggctggg gaacacggcc tcaaggtggg
     6541 tgccggtggc cagctgcagg cggtacaggg tggcggcgcc gaggaagctc ttgccgacga
     6601 tgcgggcctg cagcgcgctg tccggcgcgt ggacgatgtc gtccgggcgc agcagcatgt
     6661 ccaccgcggt gcccggtgcc cactggtagg cgcggttgcc gcggatcacg ccgagttcgg
     6721 tctgtaccgt gtcatggctg agcatctggc cgcggacgaa gtagccctgg ccgatgaagc
     6781 tggcgacgaa gggggtcagc ggctcgtggt agaggttgaa cggcgtatcc cactgctcca
     6841 ggtggccgtc cttgaacacg ccgacctggt cgctgacggc gaaggcttcc tcctggtcgt
     6901 gggtgaccag gatggcgctg gtgccgcgcg ccttgaggat ctcgcgcacc tcgtggctga
     6961 ggcggcggcg caactcgacg tcgaggttgg agaagggctc gtcgagcagc agcaggcgcg
     7021 gctctggcgc cagcgcgcgg gccagggcca cgcgctgctg ctggccgccg gacagttcat
     7081 ggggatagcg ctggcccagg gcaccgagat tgaccagctc gagcatctcg gcggcgatgc
     7141 gttcacgctg cgggtgctgg cggatgccaa aggcgatgtt ctcggccacc gtcaggtgcg
     7201 ggaacagcgc gtagtcctgg aacaccatgc cgatgcggcg cttttccggg gcgagggtga
     7261 agccggggcg ggaaatcacc tcgccggcca gctcgatgct gccctcctgc acgggttcga
     7321 agccggcgat ggcgcgcagg gtggtggtct tgccgcagcc cgagggaccg agcaggcagc
     7381 cgatgtcgcc ggcattcaga tgcaggctga cgttctgcac cacccgctgt tcacggtagc
     7441 cgcagaccag attgcggagg ttgagcagca ggcggttggg catcgggcgc ttcctgtcta
     7501 gacgttgccg gcgtgctcga cgaggaactc gagcagcgcc ttctgcgcgt gcaggcgatt
     7561 ttccgcctgg tcccaggcga ccgcgcgcgg atcgtcgagc aggtcgaggc tgatttcctc
     7621 gccacggtgg gcgggcaggc agtgcatgaa cagcacgtcg ctggcggcct ggtcgagcag
     7681 tgcgcggctg acctggtagg gtgcgaagag ctgcatgcgt gcggcgagct cttcctcctg
     7741 gcccatcgag gcccacacgt cggtgctcac cagatgcgcg ccggctaccg cctcgcgcgg
     7801 gtcgcgcacc aaggtgacgc gcccctgggc ctgctcgatg aactcgcgct tgggatcgta
     7861 gccttccggg caggcgacct tgagctggaa atcgtactgc acggcggcct gggcgtaggt
     7921 gttgcacatg ttgttgccgt cgccgaccca cgccacggtc ttgccctgga tcgagccgcg
     7981 gtgttcgtgg aaggtctgca tgtcggccag cagctggcag gggtgcagat cgtcggacag
     8041 gccgttgatc accggcaccc gcgagtgggc ggcgaagtcg aggactttct ggtgggcgaa
     8101 ggtacggatc atgatcgcgt cgagcatgct cgacatgacg atggcggagt cgctgatcgg
     8161 ctcgccgcgg ccgagctggg tgtcgcgcgg cgacaggaag atggcctggc cgccgagctg
     8221 gatcatgccg gcttcgaacg atacccgggt gcgggtcgag gccttctcga agatcatccc
     8281 cagcacgcgg ttcttcagcg gctcgtagag cacgcggcga ttgcgcaggt ctttcagctc
     8341 gatgccacgg aggatcaggc tggccagctc ctggggcgtg caatccatca acgagagaaa
     8401 gtgccgagcg ctcattattc actacctgta ggacaggccg cagaaatgac cggctttggg
     8461 cgtgagcggg cgagaactgc ggcgggaccg cactgtgcgg tgttcaacga aaaaagaagc
     8521 cgatcttata aggaaataac gtgactgcgc aaaccgccgg tttatggcgg gatggtctga
     8581 gcggcacagc tccgcggcag gggatgcaag gccgagcgtc gtgctcggct ttccgttaca
     8641 atcgccccat ttcgccggtc atgtgccgga accttcagcc gaggtagacc atggacatca
     8701 tcgagaccat taaagaacaa atcgccagca accccgtcct gctgtacatg aagggttcgc
     8761 ccaacgctcc gcagtgcggt ttctctgccc gcgcttcgca ggcggtgatg gcctgcggcg
     8821 agaagttcgc ctatgtcgac atcctgcagc acccggaaat ccgtgccagc ctgccgcagt
     8881 atgccaactg gccgaccttc ccgcagctgt gggtcgacgg cgagctggtc ggcggcagcg
     8941 atatcattct cgagatgcac gagaagggtg agctgcagcc gctgatcaag gcggccgtgc
     9001 acaagggcga gtgatcgacc ccacgccgtg ttgagttgaa aagcccctgc ccaggggctt
     9061 tttcgtttcc ggccggtcag tcgtgcatct gcgactgcaa gtagttctcc agacccacct
     9121 tgccgatcag gtcgagctgg gtttccagcc agtcgatgtg ctcttcctcg gagtcgagga
     9181 tctcttccag caggtcgcgg gtgccgtagt cggcgatgct ctcgcagtag gcgatggctg
     9241 ccttgaggtc gggaacggcc ttctgctcga tcttcaggtc gcactcgagc atttccttgg
     9301 tgtgctcgcc gatcagcagc ttgcccagat cctgcaggtt gggcaggcct tcgagaaaca
     9361 ggatgcgctt gatcagtctg tccgcatgct tcatctcgtc gatcgattcg tcgtactcgt
     9421 gcttgccgag ccgcttcagc ccccagtcct catacatccg cgcgtgcagg aagtactggt
     9481 tgatcgccac cagttcgttg ccgaggatgg tgttcaggtg ctggatgact ttcttgtcgc
     9541 ctttcatgcc gtagcccgcc tcgccggtga tggataggca aagagtcttg gtcgggtacg
     9601 ggggcctgtc aaatgcaggc ggctgcaagc ttggcgggct ccgcgaaacg gagcgccagg
     9661 atgccggggg ctggactcag gcggcggcga aggccgccgg gtgggcgagg gcgatctggg
     9721 cctggtgcac gtcgtggatg gtgtggcgca ccagcttctt ggcctcgcag gcgcacttgc
     9781 cgcactgggt gccgacgccc gtgctttcgc ggacctggcg atagctgcag caaccctcgt
     9841 ggatggcatc gcgaatctga tggtcggtga cacccaggca aagacagacg tacatggatc
     9901 tacccgcaag cagtcggttg atgctgtcga ttctggtttt aatgagaaat attgtcaaac
     9961 gcgatcaatg gccatttgtc ggttgccgat gccgcctgcc gccgcgcgca ggcatgaaaa
    10021 aacccggcaa agccgggttt ccaggaggcg gggacgcgac gggatcactc gtcgaagtcg
    10081 atccagccgc ccatttcctt ccagcggttg acgatgccgc agaacagctc ggcagtcttc
    10141 tcggtgtcgt agcgggcgtt gtgcgcctcg cggttgtcga aggggatttc cgccgcctgg
    10201 caggccttgg ccagcacggt ctggccgtag gccaggccgg cgagggtggc ggtgtcgaag
    10261 ctggagaacg ggtggaacgg gttgcgcttg atgccggtac ggttgaccgc ggcattcagg
    10321 aagccgaggt cgaagctgct gttgtggccg accagcaccg cgcgcttgca gccgctgctc
    10381 ttcagcgact tgcgcacgct cttgaagatc tcgcccagcg cctgctcctc gctcaccgcc
    10441 atgcgcagcg ggtggccgag cttgatgccg gtgaagtcca gcgccgcctg ttcgatgttg
    10501 gcgccctcga acggctcgat gcggaagaac gcggtgtgct cgggatagag caggccgtcg
    10561 tcgtccatgg cgatggtggt cgcggcgatc tccagcaggg cgtcggtggc acagttgaag
    10621 ccgccggtct cgacgtccac caccaccggc aggaagccgc ggaagcggcg cgccatcggc
    10681 ggcttgaagc cgctggtgtt gggttcctgt tcgtagtctt cgtagtcgat ctgctcgctc
    10741 atgcttcgtt ctccagcact cgccagtgca gtttctcgcc ggcgcgcagc ggcaccagct
    10801 ggtggtcgcc gaaatgcagt tcggtcgggg cgacccagtc ctcgcggatc agggtgatgg
    10861 tgtcgctgtt gcgcgccagg ccgtagaagt ccgggccgaa ctggctggcg aagccttcca
    10921 gcttgtccag cgcgccgcga cggtcgaagg cctcggcgta cagctcaatg gcggcaaacg
    10981 cggtgtagca gccggcgcag ccgcaggcgg cttccttgac gtgacgggca tgcggcgccg
    11041 agtcggtgcc gaggaagaac ttcggattgc cgctggtggc ggcatccagc agggcttcct
    11101 ggtgggtgtt gcgcttgagg atcggcaggc aatagaagtg cggacggatg ccgccgacca
    11161 gcatgtggtt gcggttgtac agcaggtgat gggcggtgat ggtggcgccg acgttggccg
    11221 gggcttcgcg cacgaactgc gcggcatcgc cggtggtgat gtgctcgaac accaccttga
    11281 gggtcggaaa gcgctcgacc acgcggcgca ggttctcgtc gatgaagcgc ttctcgcggt
    11341 cgaacacgtc gatgtcggcg tgggtcacct cgccgtgcac cagcagcggc aggccgacct
    11401 cggccatcgt ctccagtacg gcgaagatgt tgtcgacggc ggtgacgccc gagtccgagt
    11461 tggtggtggc gccggccggg tacagcttgg cggcgtgcac cagaccgccg gccttggccg
    11521 cgcggatttc ctcggggctg gtcttgtcgg tgagatagag caccatcagc ggctcgaagc
    11581 ggctgccggc cggacgggct gcgaggatgc gttcgcgata ggcggcggcc tgttcggcat
    11641 tgcgtaccgg cggcaccaga ttgggcatga tgatggcgcg gccgaaggtg cgcgcggcat
    11701 cgggcacggt gtgcgccagg gcggcgccgt cgcgcaggtg gatgtgccag tcgtcgggac
    11761 gcagcagggt caggcggtcg gacatgcggg ggaattccag gcggggtgac tcgtgggacg
    11821 tgatgctacc gaaaagccgt cggacggtca cgctgcggtg ccgttgcgcc gccgtgcggg
    11881 gcgccgcgcc cctgtaattt cgtggtttgc acggctagaa tgatcgtttt cgtagcaacc
    11941 ccccgtggag taacgaatgg cagacgtaaa aaaggtagtt ctggcctatt ccggcggcct
    12001 ggatacctcg gtgatcctca agtggctgca ggatacctac aactgcgaag tggtgacctt
    12061 caccgctgac ctgggtcagg gcgaggaagt cgagccggcg cgtgccaagg cacaggcgat
    12121 gggcgtcaag gaaatctaca tcgacgacct gcgcgaagaa ttcgtccgcg acttcgtctt
    12181 cccgatgttc cgcgccaaca ccgtgtacga aggcgagtac ctgctgggca cctccattgc
    12241 ccgtccgctg atcgccaagc gtctgatcga gatcgccaac gagaccggcg ccgacgccat
    12301 ctcccacggc gccaccggca agggcaacga ccaggtgcgt ttcgagctgg gcgcctacgc
    12361 gctgaagccg ggcgtcaagg tgatcgctcc ctggcgcgag tgggacctgc tgtcgcgcga
    12421 gaagctgatg gactacgccg agaagcacgc catcccgatc gagcgtcacg gcaagaagaa
    12481 gtcgccgtac tccatggatg ccaacctgct gcacatctcc tatgaaggcg gcgtgctgga
    12541 agacacctgg accgagcacg aagaagacat gtggcgctgg accaagtccc cggaagccgc
    12601 gccgaacgtg ccgacctaca tcgagctgac ctaccgcaac ggtgacatcg tcgccatcga
    12661 cggcgtcgag atgaccccgg cgaccgtgct gaccaagctc aaccagatcg gcggcgagaa
    12721 cggcatcggc cgtctggaca tcgtcgagaa ccgctacgtc ggcatgaagt cgcgcggctg
    12781 ctacgagacc cccggcggca ccatcatgct gcgcgcccac cgcgccatcg agtcgatcac
    12841 cctcgaccgc gaagtcgccc acctgaaaga cgagctgatg ccgaaatacg ccagcctgat
    12901 ctacaacggc tactggtgga gcccggagcg tcagatgctg cagcagatga tcgacgcctc
    12961 ccaggctacc gttaacggcg tggtgcgcct gaagctgtac aagggcaacg tcatcgtggt
    13021 cggccgcaag tccgacgact cgctgttcga cgccaacatc gccaccttcg aggacgatgc
    13081 cggcgcctac aaccaggccg atgccggtgg cttcatcaag ctcaacgccc tgcgcctgcg
    13141 catcgccgcg ggcaagggtc gcagcccgct ctgataagct gggttgaccg agcctgaaca
    13201 tgaccccggc cgcgtgccgg ggtcttgctt tgtggatgct gaaaggtagg agcgaattca
    13261 ttcgcgaccc ggaccccggt cgcgaatgaa ttcgctcctc cggtcttgag aggagaaacc
    13321 catgagactg ctgcacacca tgctgcgcgt cggcgatatg gacaagtcga tcgccttcta
    13381 caccgaagtg ctgggcatga ccctgctgcg ccgcaaggac tacccggacg gccagttcac
    13441 cctggccttc gtcggttacg gcaacgaggc ggacaacagc gtcatcgagc tgacctacaa
    13501 ctggggcgtg gacagctacg agctgggcac cggctacggc cacatcgccc tagaagtgga
    13561 ggacgtctac aaggcctgcg aggacatccg cgcccgcggc ggcaagatca cccgcgagcc
    13621 ggggccgatg aagcacggct ccagcatcct cgccttcgtc gaggacccgg acggctacaa
    13681 gatcgaactg ctgtcgccgc atcgcggcga ctgacttcgc cgctacagca gaagccccgc
    13741 catgtgcggg gcttctgctt tgtgcgtgtt cagttgccgc ggtaggtcga gtagccgtag
    13801 gggctgagca gcagcgggat gtggtagtgc ggcacgctgc cgtcggcggt gaagatcacc
    13861 ggcacttcgg ggaagaaggt cgcggcctgc tgcttggcga accactcgcc ggtcttgaag
    13921 gtcacccggt aggtgccttt ctccagcgcc tggccttcag ggaacagcgc ggtgatgcgg
    13981 ccctgcgggt tggtgacggc gctgttgagc tcggtccact gcgcgccgtc ctgcttctcc
    14041 agggtgacgg tcacgtccgg cgagggcagg ccgtcctcga ggttgagcac atgcacgctg
    14101 agcgggttgc cggcggccag ggccagggac gacaggccgc tgagcagcgc gccgacgaag
    14161 agggacttga gtgcgttcat gggattctcc ggatcagtgg gtttgcggta gcagttgggc
    14221 gatggccttg atggcgcagg cttcgtcgat ggctgcgccg ccggcgccag ccacgccgat
    14281 ggcgccgatc acttcgccgc ctacctgcag cggcacaccg ccgccgagca gcagcagttc
    14341 atcaagggtg ttgaggtttg ccgcctccgg gttggtgcgt gcccgctcgg ccagcaggcg
    14401 ggtggcggtc ttggtcgaca gcgcggtgta ggccttgcgc tgcgcggcga gggtgttgtg
    14461 cgggccgacg ttgtcgtcgc gctgcaccgc caccaggttg ccgctgcggt cgaccaccgc
    14521 cgccacggcg ctgcggccgt cggcatggca ggcggcgagg gtggcgtcga gcagctggtt
    14581 ggccagtgcc agcgaaacat cactacgttg ttcgacctga gccggggcgg cgaaggcgtt
    14641 ggcggcgcac agggccaggc caggcagcga cagccgggcg gtacggatgg gcatgggctt
    14701 ttccttcaag gggctccggg gagcgagtgg cggccatggt gcccgcgccc gcccgtcaaa
    14761 ccgattgccg gcggattacc gatttgtaat gggagaaagc ccgggatcgg cctagcctat
    14821 gcgggacttt gggggaggcc gtgatgcgca ttctggtggt ggaagacgag ctgaagaccg
    14881 cggactacct gcgcaagggc ctgggcgagt ccggctatct ggtcgaggtg gcgctgaatg
    14941 gcctggacgg gttgcacctg atccaggaaa gcgagttcga cctgatcatc ctcgacgtca
    15001 tgctgcctgg gctggatggc tggcaactgc tgcaggtgat ccgccgccgg ttgccgacgc
    15061 cggtgctgtt cctcaccgcc cgcgacgcgg tggaggatcg ggtcaagggc ctggagctgg
    15121 gcgccgacga ctacctggtc aagccgtttt cctatgccga actgctggcg cgggtgcgca
    15181 ccctgctgcg ccgcgggccg ccgcgcgagg tcgagcagtt ccgcgtggcg gacctggagc
    15241 tggatttgct gcgccgccgg gtcacccgtg cgggcgagcg cgtcaacctg accaacaagg
    15301 agttcgccct gctgcatctg ctgctcagcc gcgagggcga ggtgctgtcg cgcgcctaca
    15361 tcgcctcaca ggtctggcag atgaacttcg acagcgacac caacgtggtc gacgtggcca
    15421 tccgccggct gcgcgccaag atcgacgatc cctatccgtg caagctgatc cataccgtgc
    15481 gcggcatggg ctatgtgctg gaagtgcagc cgtgagcctg tggccgcgcg cgctgagcct
    15541 gcgcctggcg ctgctgttcg ccctggtcgg cgtgctgctg ctcggcgcca tcggcttcta
    15601 cctgtaccag tcgctggagc gggaaatcgc ctggcgcgac gaccaggcgc tgctggggcg
    15661 cctggagcgc atgcgcacgc tgctcgacga cagcgcgagc attgaggcgc tgcgccagcg
    15721 gccgcagctg tacgccaaca tgctgggcaa tcgcgacagc ctgctgtgga tcgtcagcga
    15781 cgacggacag gtgctgatcg agatcaaccc cgccgcactg gccgtgccgc agctgccgcg
    15841 tgggccggcg gtgcagctgc gcgacaccct ggtggccgag cc
//
LOCUS       DATCUN010000115         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_230133, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000115 DATCUN010000000
VERSION     DATCUN010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230133"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VIO83_04505"
     CDS             complement(<1..687)
                     /locus_tag="VIO83_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8330571.1"
                     /translation="MTTQVRPQPAYDVIVVGSGAGAMSAAVVAADQGLSVLVVEKSDK
                     YGGTSAISGGGIWIPNNHYFADKGGKDSYDKALTYLKASTEGKIDEERLRAYLEYAPR
                     MIHYLETRSRVRYAVAEKYPDYYQHLPGSLPGGRSLDPELFDTSLLGDEYDNMRKPSP
                     TTLLMGCIAWTARHAHKAMSREFGWRLMILGLIARYKLDFKWRRKTKMDRRAALGASL
                     IAALRRSLMDR"
     gene            complement(731..1918)
                     /locus_tag="VIO83_04510"
     CDS             complement(731..1918)
                     /locus_tag="VIO83_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016781268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8330572.1"
                     /translation="MHVMREKTAVEIELIARARRLVPALRERAERADREGKVPDETIR
                     EMQEAGLFRALQPKQWDGYEVDPRTFFEIQMTLAEGCMSTAWIYGVMGVHPWQLARYP
                     VEAQQDVWGEDTSTLISSTYMPVAKVTPVEGGYRISGRWGFSSGSEHCQWGLLGGILP
                     ADGDLPVEHGTFLVPRSDYRIEKNWDVLGLRGTGSHDIVIEDAFVPAHRVQRTNNSSL
                     AATPGREVNTNPIYALPFAQVFTRAVSSSCLGALQGAINEFRDNAARHIGKHGAKTAE
                     DPVAQTAVAEAITTVDAFKLVLERNFAHMLALAEKGEIPDTETRLLYRYQSAQVTNIC
                     AERVSDLLRSMAASGLYNTNPVARTFRDLHQARGHISNNVAAYGRSYGTVQLGLPNPD
                     PYV"
     gene            complement(2187..>2388)
                     /locus_tag="VIO83_04515"
     CDS             complement(2187..>2388)
                     /locus_tag="VIO83_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HEY8330573.1"
                     /translation="AAMQDAPFGGIGASGMGHYHGREGFLEFSHARTVFEAGSYDPRR
                     EWGLLPPYGEHFLAAMEAQVTP"
BASE COUNT          348 a          754 c          867 g          419 t
ORIGIN      
        1 gcggtccatc agcgagcggc gcagcgcggc gatcagcgag gcgccgaggg cggcgcggcg
       61 gtccatcttg gtcttgcgcc gccacttgaa gtcgagcttg tagcgggcga tcaggccgag
      121 aatcatcagc cgccagccga actcgcggga catggccttg tgcgcgtggc gcgcggtcca
      181 ggcgatgcag cccatcagca gggtggtcgg cgacggcttg cgcatgttgt cgtattcgtc
      241 gccgagcagg ctggtgtcga acagctcggg gtccaggctg cgcccgccgg gcagcgagcc
      301 gggcaggtgc tggtagtagt ccgggtactt ctcggccacc gcatagcgca cgcggctgcg
      361 ggtctcgagg tagtggatca tccgcggcgc gtactcgagg taggcgcgca ggcgctcctc
      421 gtcgatcttg ccctcggtgg aggccttcag gtaggtcagc gccttgtcgt agctgtcctt
      481 gccacccttg tcggcgaagt agtggttgtt ggggatccag atgccgccgc cggagatcgc
      541 cgaggtgccg ccgtacttgt cgctcttctc gacgaccagc accgagaggc cctggtcggc
      601 ggcgaccacc gctgccgaca tggcgcctgc gccggagccg acgacgatca cgtcgtaggc
      661 gggttggggc cgaacctgtg ttgtcattgt tttcgtgtcc tttgccgtgg tggtcggtga
      721 gccgggtcga tcagacgtac ggatcggggt tgggcaggcc gagctgcacc gtgccgtagc
      781 tgcgcccgta ggccgccacg ttgttggaga tgtgcccgcg cgcctggtgc aggtcgcgga
      841 aggtgcgcgc caccgggttg gtgttgtaca gaccggaggc ggccatcgag cgcagcaggt
      901 cgctgacccg ctcggcacag atattggtga cctgggcgga ctggtagcgg tacagcaggc
      961 gggtctcggt gtcgggaatc tcgcccttct cggccagcgc cagcatgtgt gcgaagttgc
     1021 gctccaacac cagcttgaag gcgtcgacgg tggtgatcgc ctcggccacc gcggtctgcg
     1081 ccaccgggtc ctcggcggtc ttggcgccgt gcttgccgat gtggcgggcg gcgttgtcgc
     1141 ggaactcgtt gatcgcaccc tgcagggcgc cgaggcacga gctggacacc gcgcgggtga
     1201 acacctgggc gaagggcagc gcgtagatcg ggttggtgtt gacctcgcgg cccggggtag
     1261 ccgccaggct ggaattgttg gtgcgctgca cgcggtgcgc ggggacgaag gcgtcctcga
     1321 tcacgatgtc gtggctgccg gtgccgcgca ggcccaacac gtcccagttc ttctcaatgc
     1381 ggtagtcgct gcgcggtacc aggaaggtac cgtgctcgac cggcaggtcg ccgtcggccg
     1441 gcaggatgcc gccgagcagg ccccactggc agtgctcgct gccgctggag aagccccagc
     1501 gcccgctgat gcggtagccg ccttccaccg gggtgacctt ggccaccggc atgtaggtgg
     1561 aggagatcag cgtgctggtg tcctcgcccc acacgtcctg ctgggcctcg accgggtagc
     1621 gggccaactg ccaggggtgt acgcccatca cgccgtagat ccaggcggtg gacatgcagc
     1681 cttcagcaag ggtcatctgg atttcgaaga aggtgcgcgg gtcgacctcg tagccgtccc
     1741 actgcttggg ctgcagggcg cggaacaggc cggcctcctg catctcgcgg atggtctcgt
     1801 cgggcacctt gccctcgcgg tcggcccgtt cggcccgctc gcgcagggcg ggaaccaggc
     1861 ggcgggcgcg agcgatcagc tcgatctcta ccgcggtttt ctctcgcatg acatgcatcg
     1921 ccaactcctc catccatttc gatgagcgaa tctgctcgtg gcttcgatca tcgggcgcgg
     1981 cccggctgcg ctcatcgtca aaccggacta ggcggaatcg tgcagacggc ggacagcaca
     2041 aggcccggcg ctggggccgg gcctttggga agatgcgtat gcaaaatcag acgtaaggtg
     2101 gaagactcgc gcagcgatct tccaccatga ggcgcgcaac tggtggaaaa gacctgcggt
     2161 cgttttccac cctacgactg cgcaccctac ggggtgacct gcgcctccat ggcggcgagg
     2221 aagtgttcgc cgtagggcgg cagcagcccc cactcgcggc gcgggtcgta actgcctgcc
     2281 tcgaacacgg tgcgcgcgtg gctgaactcg aggaagccct cgcggccgtg gtagtggccc
     2341 atgccggaag cgccgatgcc gccgaacggg gcgtcctgca tggcggcg
//
LOCUS       DATCUN010000116        11632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_192923, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000116 DATCUN010000000
VERSION     DATCUN010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11632
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192923"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VIO83_04520"
     CDS             complement(<1..312)
                     /locus_tag="VIO83_04520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8330574.1"
                     /translation="MSVTAPRVRIQALTPAHAAAARQWAERLGLGEEGEAEFALQVGD
                     EGLQLAELGPDAPGPVRVDFVEGGAAHRRRFGGGAGQMIAKAVGVQPGVRPRVLDATA
                     GL"
     gene            complement(391..1245)
                     /locus_tag="VIO83_04525"
     CDS             complement(391..1245)
                     /locus_tag="VIO83_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8330575.1"
                     /translation="MPLPRLALLTALAMLAFAANSLLCRIALRDGHIDAASFTAIRLL
                     GGALALWLIVRLRPAAPRAPLGGSHLSALALFVYAAGFSWAYVQLSAATGALLLFGAV
                     QASMILWGLWRGERLSAGQGCGLLLAFAGLAVLLLPGASAPAALPAMLMLLAGVAWGA
                     YSLRGRGAGDATAHTAGNFIRALPLALLLGLVGLPWAQLDAAGLGYALLSGALASGVG
                     YALWYAVLPALGATQAATIQLSVPLLTAVLGVLLLGEAFDRRLLLAAVLLLGGVAWVL
                     LGRSPARR"
     gene            complement(1415..2269)
                     /locus_tag="VIO83_04530"
     CDS             complement(1415..2269)
                     /locus_tag="VIO83_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011059499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY8330576.1"
                     /translation="MLPYFLGCPSWNEPAWRGSLYPLGLPSRDFLSAYGRVFNAVEGN
                     TTFYARPAATTLARWAQLMPVGFHFCAKLPRDISHEGDLRANLAAAGEFLDLLAPLGA
                     RVTPLWLQLPASFGPPRLGELAAFIDGLPSARSLAVEVRHPVFFARGDEEKALNRLLL
                     ERGVERICLDSRPLFSCAARTPAVLHAQSKKPRVPPRPAAFTSFPQVRFIGHPQLEAN
                     DAFLAPWLDKVAGWIEEGRTPHVFLHTPDNHRAPELARRFHGQLMARLPGLPALADSG
                     GGEQLDFL"
     gene            complement(2277..2951)
                     /gene="tsaB"
                     /locus_tag="VIO83_04535"
     CDS             complement(2277..2951)
                     /gene="tsaB"
                     /locus_tag="VIO83_04535"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY8330577.1"
                     /translation="MTTLLALDTATEACSVALLHEGRVFSRYEVIPRLHAQRVLPMVQ
                     ELLAEAGIALAAVEAIAFGRGPGAFTGVRIAVGVVQGLAFALDRPVLPISNLAVMAQR
                     AHREQGATQVATAIDARMDELYWGCYRLEDGEMRLAGVEAVLPPEQVALPRGAEGEWF
                     GAGTGWAFASRIPVLVQAMDAGLLPHAEDLLTLATFAWARGEAVVADQAQPVYLRDNV
                     ATPKKA"
     gene            complement(3052..3699)
                     /gene="adk"
                     /locus_tag="VIO83_04540"
     CDS             complement(3052..3699)
                     /gene="adk"
                     /locus_tag="VIO83_04540"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY8330578.1"
                     /translation="MRVILLGAPGAGKGTQARFITEKFGIPQISTGDMLRAAVKAESP
                     LGLQVKEVMASGGLVSDEIIIGLIKERIVAADCANGFLFDGFPRTIPQAEALRDAGVE
                     IDHVLEIAVEDAEIISRMSGRRVHPASGRTYHLQYNPPKVAGKDDETGEELIQREDDL
                     EETVRKRLDVYHAQTMPLVGFYQDLAHTAGKPKYSRVEGVGSVEEITAKVLAALS"
     gene            complement(3866..6502)
                     /gene="ppc"
                     /locus_tag="VIO83_04545"
     CDS             complement(3866..6502)
                     /gene="ppc"
                     /locus_tag="VIO83_04545"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016980983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY8330579.1"
                     /translation="MAEIDARLREDVHLLGELLGDTFRAERGAEFLAKIERIRQGAKT
                     ARRGSAEGARQLAQTLDALAEDELLPVARAFNQFLNLANIAEQYHRIRRRRADEPLPF
                     EERLLGELLERLQAAGHSRQDLARQIAGLDIELVLTAHPTEVTRRTLIQKYDAIAACL
                     AARDHADLSLREQVRVRRELCRLLAEVWHTEEIRRSKPTPVDEAKWGFAVIEHSLWRA
                     LPDFLRGVDELLHGCCGTRLPLAATPVRFASWMGGDRDGNPNVTAAVTVEVLLQARRL
                     AAELHLRDVERLITDLSMHQASPALRERVGETPEPYRALLRELRTRLFATRDWAAAAL
                     LDGRAPDARVLQDNGELLEPLLLCYDSLTACGMGSIADGPLLDSLRRAHTFGLFLARL
                     DIRQDAGRHVAALDEITAYLGLGRYAEWDEGQRLVFLQRELASPRPLLPLHCRPSADT
                     AEVLATCRVVAQAPAAALGSYVISMAGAPSDVLAVQLLLKEAGLQRPLRVVPLFETLE
                     DLEHAGAAIDRLLAVPGYREGLAGPQEVMIGYSDSAKDAGTVAAAWAQYRAQEALVAV
                     CARHGVELLLFHGRGGTVGRGGGPSHAAILSQPPGSVAGRFRVTEQGEVIRFKFGLPG
                     VAEQNLNLYLAAVLEATLLPPPTPTAEWRALMERLAADGVAAYRAVVREHPQFVEYFR
                     QATPELELGRLPLGSRPARRRSGGVESLRAIPWIFAWTQTRLMLPAWLGWEAALERAV
                     ARGEAELLREMRAHWPFFRTRIDMLEMVLVKADADIARLYDERLVEENLRGLGQHLRD
                     LLSQAVRMVLGLTGQAQLLGNDPVLRESISVRDPYLDPLHLLQVELLARSRKLAEHGE
                     SPLEQALLVSVAGIAAGLRNTG"
     gene            6632..6976
                     /locus_tag="VIO83_04550"
     CDS             6632..6976
                     /locus_tag="VIO83_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin assembly protein"
                     /protein_id="HEY8330580.1"
                     /translation="MKIRDLVNEWELSAKGTLSKSTFCIPLDLESAARLAALKEMYPK
                     RSTEELLGGLLGAALQELEESFPYVKGSKVVTTDEQGDPLYEDIGLTPRFLALARKHR
                     QDLSQRDDDDDF"
     gene            7198..8493
                     /locus_tag="VIO83_04555"
     CDS             7198..8493
                     /locus_tag="VIO83_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEY8330581.1"
                     /translation="MPRASRLLLLLAGLWLGNAQAAVNEIDAVSYGFPLTNPFEATIA
                     TAPPELRPVLPDDADIDQADYSLRLRPRRELPDNFWPVKSLKYRLARQDGPAPLVFII
                     SGTAAHYANDKTEDLKRLFYGAGYHVVQLSSPTSYDFMAAASRHATPGISDRDAIDLY
                     QVMERIRGQQSDLPVTGYDLVGYSLGGLNAAFVSHLDESRKTFNFRRVLLINPPVNLY
                     TSVANLDRLVQTEVKGVDNSSTFYDLVLGKLARYFEERGTAQIDAAMLYDFQQSKQRL
                     SDEEMAMLIGTVFRFAAADIAFTSDLINRRGFIIPPTYPMDEGTSLTPFFKRALLCDF
                     QCYIREQLMPYWRRQGDGKSLLQMIDQVSLYSLRTYLAGSDKIRVFHNRDDLILSPGD
                     LGFLRRTFGERLTLFPYGGHCGNMNYRVNTQAMLEFLRD"
     gene            8510..9259
                     /locus_tag="VIO83_04560"
     CDS             8510..9259
                     /locus_tag="VIO83_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEY8330582.1"
                     /translation="MLLGALLTLSGIAAAEPDADGFARPLETLKFNPQLNQHEFERAT
                     LVALVMNDPWEEWNRRIYHFNQRFDEWVYLPLVNGYRFVTPRFLRSGVSHFFANLGEV
                     PSLANHLLQLEGRDALHTTTRLLFNTILGVGGLWDPASHMGLERRSEDFGQTLGYWGA
                     EEGPYMVLPLLGPSNLRDTGGLAFDSTVGSALDLANLSGLTARPAEYYSLMAIDRRHI
                     NDFRYGQLNTPFEYEKVRYVYTEARRLQIRE"
     gene            9368..10228
                     /locus_tag="VIO83_04565"
     CDS             9368..10228
                     /locus_tag="VIO83_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY8330583.1"
                     /translation="MSYPHYRDILDLHSGRATSDGDGVRLTRLIGGAGLERFDPFLML
                     DAFDSFDARDYIGGFPPHPHRGFETVTIMIEGRMRHEDHLGHRGLLEPGGVQWMTAAR
                     GIVHSEMPEQEQGRMFGFQLWLNLPAADKMGPAGYRDFAPEEIPQLTSVHGVGVRVIA
                     GHFKEGEQEQAGAVQRPHTEPQLFDLSFSAGACLMPELPRGHNVLLHVFEGELTVRGQ
                     RDQNIASGQLVRLGDGDSLHLTSASGARALLIAGRPLREPIVQYGPFVMNSREEIEQA
                     LDDYRNGRFA"
     gene            complement(10251..11174)
                     /locus_tag="VIO83_04570"
     CDS             complement(10251..11174)
                     /locus_tag="VIO83_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEY8330584.1"
                     /translation="MERPRFRAAFLHPRFWLLWLGLGLLWLVVQLPYKTLLKLGQALG
                     VLMLLGARSRRHIAARNLELCFPELSASERAHLLRENFASSGIAFFEMAMSWWWPRAR
                     LARLAHVEGLEHLQAALREGQGVILMSLHFTTLEIGAALLGQRHTIDGMYREHKNPLF
                     DYIQRHGRERHNLDASAIERDDVRAMLKVLRAGHAIWYAPDQDYGRKQSIFVPLFGIP
                     AATVTATSKFARLGKARVIPFTQQRLADGSGYRLTIHPPLQDFPGASEEADCLRINQW
                     VEQAVRECPEQYLWAHRRFKTRPEGEPKLYG"
     gene            11307..>11632
                     /gene="minC"
                     /locus_tag="VIO83_04575"
     CDS             11307..>11632
                     /gene="minC"
                     /locus_tag="VIO83_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109174.1"
                     /note="blocks the formation of polar Z-ring septums;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEY8330585.1"
                     /translation="MNLADLTDQDPVFRLKGNMLAITVLELARNDLALLDRQLAEKVA
                     KAPNFFHDAPVLLGLEHLAETDAELDLGSLLDICHRHGLLALGARASRPGDIAAAEEL
                     GLPLLPP"
BASE COUNT         1989 a         4122 c         3865 g         1656 t
ORIGIN      
        1 gagaccagcg gtggcatcca gcacccgcgg gcgcacgccg ggctgcacgc cgaccgcctt
       61 ggcgatcatc tgcccggcgc caccaccgaa gcgccggcga tgggccgcgc cgccctcgac
      121 gaagtcgaca cgcaccggcc ccggtgcatc cgggccgagc tcggccagct gcaggccttc
      181 gtcgcccacc tgcagggcga actcggcctc gccctcctcg cccagcccga gccgctcggc
      241 ccactggcgg gcggcggcgg catgggcagg ggtgagggct tggatccgga ctcggggggc
      301 agtgacagac atcggggctt ccgcgaaatg ggcagagcat cgctggcgat gcgggggccg
      361 ccattttgcc aaagaaagcg ccggcctgtc ctatcgcctg gctggcgaac gccccagcag
      421 cacccaggcc accccgccca gcagcagcac cgcggccagc agcaggcgcc ggtcgaaggc
      481 ctcacccagc aacagtacgc ccagcacggc ggtgagcagc ggcacgctga gctggatggt
      541 cgcagcttgg gtggcgccca gtgccggcag cacggcatac cagagggcat agccgacacc
      601 ggacgccagc gcgccggaca gcagcgcata gccaaggccg gcggcatcca actgcgccca
      661 cggcaggccg accaggccga gcagcaacgc cagcggcagc gcacggatga agttgccggc
      721 cgtatgcgcg gtggcatcgc cggcgccacg cccgcgcagg gaatacgcgc cccaggccac
      781 cccggccagc agcatcaaca tggccggcag cgcagccggc gcactggctc cgggcaacag
      841 cagcacggcc aggccggcaa aggccagcag caggccgcaa ccctgccctg ccgacaggcg
      901 ctcgccgcgc cacaaccccc agaggatcat gctggcctgc accgcaccga acagcaacag
      961 ggcaccggtg gcggcggaca gttgcacata ggcccaggaa aaccctgccg catagacgaa
     1021 cagcgccagc gccgagagat ggctgccacc cagcggcgct cgcggcgccg ccggccgcag
     1081 gcgcacgatc aaccacagcg ctagcgctcc accgagcagg cggatggcag tgaagctggc
     1141 agcgtcgata tgaccatcgc gcagagcgat gcggcacagc agcgagttgg ccgcgaaggc
     1201 cagcatggcc agggcggtga gcaacgccag gcgcggcagg ggcatggcag actttccggt
     1261 cagtggtggt gcaccgaggg tagcgcgaaa ggtgcggcag cagggtgcga ctttgggctg
     1321 ccgcctcgcg gcgcagcggg tggacaggcg cccgtagggt gcgccgcgcg caccaacaac
     1381 cgacgacagc ggcttatggt gcgcagggcg caccctacag gaagtcgagc tgctcgcccc
     1441 cgccgctgtc tgccagcgcc ggcagaccgg gcaggcgcgc catcagctgc ccgtggaaac
     1501 gccgcgccag ctcgggcgcc cggtggttgt ccggggtatg caggaagacg tgcggggtgc
     1561 ggccttcctc gatccagccg gcgaccttgt ccagccaggg ggcgaggaag gcatcgttgg
     1621 cctcgagctg cggatgaccg ataaaacgca cctgtggaaa cgaggtaaaa gccgccgggc
     1681 gcggcggcac gcgcggcttc ttgctctgcg cgtgcagcac cgccggcgtg cgcgcggcgc
     1741 agctgaacag cggccgcgaa tcgaggcaga tccgctcgac gccgcgctcc agcagcaggc
     1801 ggttgagcgc cttctcctcg tcaccgcggg cgaagaacac cgggtggcgc acctccaccg
     1861 ccaggctgcg cgcggacggc agtccgtcga tgaaggccgc cagctcgccg agacgcggcg
     1921 ggccgaagct ggccggcagc tgcagccaca gcggcgtgac ccgcgcaccg agcggcgcca
     1981 gcaggtcgag gaactcgccg gcggcggcga gattggcgcg caggtcgcct tcgtggctga
     2041 tgtcgcgcgg cagcttggcg cagaagtgaa agcctaccgg catcaactgc gcccagcgcg
     2101 ccagggtcgt cgccgcagga cgggcgtaga aggtggtgtt gccttccact gcgttgaaca
     2161 cccggccgta ggcggacaga aagtcacgac tgggcaagcc gagcggatac agcgaaccgc
     2221 gccaggccgg ctcgttccac gatggacagc cgaggaagta cggcagcacc gccggctcag
     2281 gccttcttcg gcgtggcgac gttgtcgcgc aggtacaccg gttgcgcctg gtcggcgacc
     2341 accgcctcgc cacgtgccca ggcgaaggtg gcgagggtca gcaggtcctc ggcgtgcggc
     2401 agcaggccgg catccatggc ctgcaccagc accgggatgc gcgaggcgaa ggcccagccg
     2461 gtgccggcgc cgaaccattc gccctcggcg ccacgcggca gcgcgacctg ctccggcggc
     2521 agtaccgcct ccaccccggc caggcgcatc tcgccatctt ccaggcgata gcagccccaa
     2581 tacagctcgt ccatccgcgc atcgatggcg gtcgccacct gggtcgcgcc ctgctcgcga
     2641 tgggcgcgct gggccatcac ggccaggttg gagatcggca gcactgggcg gtcgagggca
     2701 aaagccaggc cctgcaccac gccaacggca atgcgcacgc cggtgaaggc gcccgggcca
     2761 cggccgaagg cgatggcctc caccgcagcg agggcgatgc cggcctcggc cagcagctcc
     2821 tgcaccatcg gcagcacgcg ctgggcatgc aggcgcggaa tcacctcgta gcggctgaag
     2881 acacgcccct cgtgcagcag tgcgacggaa caggcttcgg tggcggtatc cagggccagc
     2941 agggtggtca tgacggaggt ccggtacggt caaaaacggg cgggcattgt aacagcactg
     3001 cagaatgcaa acggcccgca caaggcgggc cgtcggcaca gcacgagcgg atcagctcag
     3061 cgcggcgagc accttggcgg tgatctcttc gacgctgccg acgccctcga cgcgggagta
     3121 cttgggcttg ccggcggtgt gcgccagatc ctggtagaag ccgaccagcg gcatggtctg
     3181 ggcgtggtac acgtccaggc gcttgcgcac ggtttcttcc aggtcgtctt cgcgctggat
     3241 cagctcctca ccggtctcgt catccttgcc ggccaccttc ggcgggttgt actgcaggtg
     3301 gtaggtgcga ccggaagccg ggtggacgcg gcggccggac atgcggctga tgatttccgc
     3361 atcttccacg gcgatttcca gcacatggtc gatttccacg ccggcatcgc gcagcgcttc
     3421 ggcctgcggg atggtgcgcg ggaagccgtc gaacaggaag ccgttggcgc agtcggcggc
     3481 gacgatgcgc tccttgatca ggccgatgat gatctcgtcg gagaccaggc cgccgctcgc
     3541 catcacttcc ttgacctgca ggcccagcgg gctctcggcc ttgaccgcgg cacgcagcat
     3601 gtcgccggtg gagatttgcg gaatgccgaa tttctcggtg atgaagcgag cctgagtacc
     3661 tttgccggca cccggcgccc ccagcagaat cacacgcatc gtattgctcc agaaaggatg
     3721 taggaaatct tggattcgcc gcgtggggcc aatctcgaaa tttttgtttt cgcccctagc
     3781 atgaaatcgg gcgctgaaaa agcagaccaa aatacacagc tactctgcag gacacaagat
     3841 gcgcacggat ctacgctaag ccgtttcacc cggtgttacg cagccccgcg gcgatgcccg
     3901 cgacactcac cagcagggcc tgttccagcg gactttcgcc gtgttcggcg agcttgcgcg
     3961 aacgcgccag aagctccacc tgcagcaggt gcagggggtc gagataggga tcgcgcacgc
     4021 tgatcgactc gcgcagcacc gggtcgttgc ccagcagctg cgcctgcccg gtcagcccca
     4081 gcaccatgcg caccgcctgc gacaacaggt cgcgcaagtg ctgccccaat ccgcgcaggt
     4141 tttcctcgac cagacgctcg tcatacaggc gcgcgatgtc ggcatcggcc ttgaccagca
     4201 ccatctccag catgtcgatc cgcgtgcgga agaacggcca gtgcgcacgc atctcgcgca
     4261 gcagctcggc ctcgccgcgc gccaccgccc gctccagcgc ggcctcccag ccaagccagg
     4321 ccggcagcat cagccgcgtc tgggtccagg cgaagatcca cggaatcgcc cgcaggctct
     4381 ccaccccgcc gctgcgccgg cgcgccggtc gactgcccag cggcaggcgg cccaactcca
     4441 gctccggcgt ggcctggcgg aaatactcga cgaactgcgg atgctcgcgc accacggcac
     4501 gataggcggc caccccgtcg gcggccaggc gctccatcag tgcacgccac tcggcggtcg
     4561 gcgtgggtgg cggcagcagg gtggcctcca gcacggcggc cagataaagg ttgaggttct
     4621 gctcggcgac gccgggcagg ccgaacttga agcggatcac ctcgccctgt tcggtgacgc
     4681 gaaaacgccc ggccaccgag ccgggcggct gcgagaggat ggccgcatgc gacgggccgc
     4741 cgccgcgccc cacggtgccg ccacgaccat ggaacagcag cagctccacg ccatgccggg
     4801 cgcacaccgc caccagcgcc tcctgggcgc ggtactgcgc ccaggccgcg gcgacggtgc
     4861 cggcatcctt ggccgagtcg gagtagccga tcatcacctc ctgcggcccg gccaggcctt
     4921 cccggtagcc gggcacggcc agcaggcggt cgatcgccgc gccggcatgc tccagatcct
     4981 ccagcgtctc gaacagcggc acgacgcgca gcgggcgctg cagtccggcc tccttgagca
     5041 gcagctgcac cgccagcaca tcgctcggtg ccccggccat ggagatcaca taggaaccca
     5101 gtgcggcagc cggcgcctgc gccaccaccc ggcaggtcgc cagtacctcg gcggtgtccg
     5161 cggacggccg gcagtgcagc ggcagcagtg gccgcggact ggccagttcg cgctggagaa
     5221 acaccaggcg ctgcccctcg tcccactcgg catagcggcc caggccgagg tacgcggtga
     5281 tctcgtcgag ggcggcgaca tgccggccgg cgtcctggcg gatgtccagg cgcgccagga
     5341 acaggccgaa ggtgtgcgcg cggcgcaggc tgtcgagcag cgggccgtcg gcaatgctgc
     5401 ccatcccgca ggcggtcagc gagtcgtagc agagcagcag cggctcgagc agctcgccat
     5461 tgtcctgcag cacccgcgca tccggcgcgc gaccatccag cagggccgcc gccgcccagt
     5521 cgcgggtggc gaacagccgc gtgcgcagct cgcgcaacag cgcgcggtac ggctccggcg
     5581 tctcgccgac ccgctcgcgc agggccggac tggcctggtg catggacagg tcggtaatca
     5641 gccgctcgac gtcgcgcaag tgcagctcgg cggccaggcg ccgcgcctgc agcagcacct
     5701 cgacggtgac cgcggcggtg acgttgggat tgccgtcgcg gtcgccgccc atccacgagg
     5761 cgaaacgcac cggtgtcgcc gccagcggca ggcgcgtgcc gcagcagcca tgcagcagct
     5821 cgtcgacgcc acgcaggaag tccggcagtg cgcgccacag cgaatgctcg atcaccgcaa
     5881 agccccactt ggcctcgtcc accggggtcg gcttgctgcg gcgaatctcc tcggtatgcc
     5941 agacctcggc gagcaggcgg cacagttcgc ggcggacccg gacctgctcg cgcagcgaca
     6001 ggtcggcgtg gtcacgcgcc gccaggcagg cggcgatggc gtcgtacttc tggatcaggg
     6061 tgcgccgggt cacctcggtg ggatgcgcgg tgagcaccag ttcgatatcc agcccggcga
     6121 tctgccgcgc caggtcctgc cggctgtgcc cggcggcctg cagacgctcg agcagctcgc
     6181 ccagcagccg ctcctcgaac ggcagcggct cgtccgcccg gcgccggcgg atgcgatggt
     6241 actgctcggc gatgttggcc aggttgagaa actggttgaa cgcgcgcgcc accggcaaca
     6301 actcgtcctc ggccagggcg tcgagggtct gcgccagctg ccgcgcccct tccgccgagc
     6361 cgcgccgcgc cgtcttggcg ccctggcgga tgcgctcgat cttggccagg aactccgcgc
     6421 cgcgctcggc gcggaaggtg tcgccgagca gctcgcccag caggtgcaca tcctcacgca
     6481 gtcgcgcgtc gatttccgcc atgcccgttc tcccgcccac gcttcgttgc tgccagtctc
     6541 acccacaggg ctgcacgagt gccaatggca gtctaggctt tttctcagga cacctgcgtt
     6601 accgacagca accccaggcc gggaggcatt gatgaagatc cgcgatctgg tcaacgagtg
     6661 ggaactcagc gccaagggga cgctgagcaa gagtaccttt tgcattccac tggatttgga
     6721 gtcggccgca cgcctcgcgg ccctcaagga aatgtatccc aagcggagca ccgaggaact
     6781 gctgggcgga ctgcttggcg ccgcgctgca ggaactggag gaaagctttc cctatgtgaa
     6841 gggcagcaag gtggtgacca ccgacgaaca gggtgatccg ctgtacgagg acatcgggct
     6901 gaccccccgc ttccttgccc tcgcccgcaa acatcggcag gatctgagcc agcgcgacga
     6961 cgacgacgac ttctgagccg accggctcag atacagtccc gcgaggggat tgttgctgga
     7021 acggacctct ccgctctggc caaagcccgt ggctggaatt tgccacggca aaccgcccat
     7081 actcgcgcca agtgtgccgt tcgtccgacc ccgctggtcc gaatgctgcg gaccgcgcgg
     7141 caactcgccg tgcctatgcg agtctgatgc tgcctggatc actgctcaag gacccccatg
     7201 ccccgcgctt cacgccttct cttgctcctt gccggcctct ggctgggcaa cgcccaggcc
     7261 gccgtcaacg agattgacgc cgtgagctac ggcttcccgc tgaccaaccc gttcgaggcg
     7321 accatcgcca ccgcgccgcc ggagttgcgc ccagtcctgc cggacgatgc cgacatcgac
     7381 caggccgact acagcctgcg cctgcgcccg cggcgtgagc tgccggacaa cttctggccg
     7441 gtcaagtcgc tgaagtaccg cctggcccgc caggacggcc cggcaccgct ggtgttcatc
     7501 atctccggca ccgctgcgca ctacgccaac gacaagaccg aagacctgaa acggctgttc
     7561 tacggcgccg gctaccatgt ggtgcagctg tcctcgccga ccagctacga cttcatggcc
     7621 gccgcctcgc gccatgccac gccgggcatt tccgaccgcg atgccatcga cctgtaccag
     7681 gtgatggaga gaatccgcgg gcagcagagt gatctgccgg tgaccggcta cgacctggtc
     7741 ggctacagcc tcggcggcct gaacgccgcc ttcgtcagcc acctcgatga aagccgcaag
     7801 acgttcaact tccgccgggt gctgttgatc aacccgccgg tgaacctgta cacctcggtg
     7861 gccaatctcg accgcctggt gcagaccgag gtcaagggcg tcgacaacag cagcaccttc
     7921 tacgacctgg tcctcggcaa gctggcgcgc tacttcgagg agcgcggcac ggcgcagatc
     7981 gacgcggcga tgctctacga cttccagcag tcgaagcagc ggctctccga cgaggagatg
     8041 gccatgctga tcggcaccgt cttccgcttc gccgcggccg atattgcctt cacctcggac
     8101 ctgatcaacc gccgcggctt catcatcccg ccgacctatc cgatggacga gggcaccagt
     8161 ctcaccccct tcttcaagcg cgccctgctc tgcgacttcc agtgctacat ccgcgagcag
     8221 ctgatgccgt actggcgccg gcagggtgac ggcaagagcc tgctgcagat gatcgaccag
     8281 gtcagcctct actcgctgcg cacctacctg gccggcagcg acaagatccg cgtgttccac
     8341 aatcgcgacg acctgatcct cagcccgggt gatctcggtt tcctgcgccg caccttcggc
     8401 gaacgcctga cactgttccc gtacgggggg cactgcggca acatgaacta tcgggtcaac
     8461 acccaggcca tgctggagtt cctccgtgac tgagcgttcc ctgcgcgccc tgctgctggg
     8521 cgccctgctg accctgagcg gcatcgccgc ggccgagccc gacgccgacg gcttcgcccg
     8581 gccactggag acgctgaagt tcaacccgca gctcaatcag cacgagttcg agcgcgccac
     8641 cttggtcgcg ctggtgatga atgacccctg ggaggagtgg aaccggcgca tctaccactt
     8701 caaccagcgc ttcgacgaat gggtctacct gccgctggtc aatggctacc gcttcgttac
     8761 cccgcgcttc ctgcgcagcg gcgtgagcca cttcttcgcc aacctcggcg aggtccccag
     8821 cctggccaac cacctgctgc agctcgaggg ccgcgacgcc ctgcacacca ccacgcgcct
     8881 gctgttcaat accatcctcg gcgtcggcgg gctgtgggac ccggccagcc acatgggcct
     8941 agagcggcgc agcgaggact tcggccagac gctcggctat tggggcgccg aggaaggccc
     9001 ctacatggtg ctgccgctgc tcggcccgtc caacctgcgc gataccggcg ggctggcctt
     9061 cgacagcacc gtgggcagcg cgctggacct cgccaacctc agcggcctca cggcacggcc
     9121 ggccgagtac tacagcctga tggccatcga ccgccggcac atcaacgact tccgctacgg
     9181 ccagctcaat acgccgttcg agtacgagaa ggtgcgctac gtctacaccg aggcgcggcg
     9241 cctgcagatc cgcgagtgag acagccaatc aatcgatcca gccaataggt ttggcgcaaa
     9301 aacagcaccc aacgatccag cgggctgatc cagaatgatg gtctggcctg aatcaaggag
     9361 ctgcgtcatg agctaccccc actaccgcga catcctcgac ctgcacagcg gccgcgccac
     9421 ctcggacggc gatggcgtgc gcctgacccg cctgatcggc ggtgccggtc tggaacgctt
     9481 cgaccctttc ctgatgctgg acgccttcga ctccttcgac gcccgcgact acatcggcgg
     9541 cttcccgccg cacccgcacc gcggcttcga gaccgtgacg atcatgatcg agggacgcat
     9601 gcgccatgaa gaccacctcg gccaccgcgg cctgctcgaa cccggcggcg tgcagtggat
     9661 gaccgcggcg cgcggcatcg tgcacagcga gatgcccgag caggagcagg gccgcatgtt
     9721 cggcttccag ctctggctca acctgccggc ggcggacaag atgggcccgg ccggctaccg
     9781 cgacttcgcg ccggaggaga ttccgcagct gaccagcgtg cacggcgtcg gggtgcgggt
     9841 gatcgccgga cacttcaagg aaggcgagca ggagcaggcc ggcgccgtgc agcgcccgca
     9901 taccgagccg cagctgttcg acctgagttt ttccgccggc gcctgcctga tgccggagct
     9961 gccgcgcggc cataacgtgc tgctgcacgt attcgaaggc gagctgaccg tgcgcggcca
    10021 gcgcgaccag aacatcgcca gcggccagct ggtacgcctg ggcgatggcg acagcctgca
    10081 cctgaccagt gccagcggcg cccgtgcgct gctgatcgcc ggacggccgc tgcgcgagcc
    10141 gatcgtgcag tacggtccgt tcgtgatgaa cagccgggag gaaatcgaac aggcgctgga
    10201 cgactaccgc aacgggcgct tcgcctgagc agcgcgccag cgccgtctgg tcagccgtag
    10261 agcttcggct caccctccgg gcgggtcttg aagcgacggt gggcccacag gtactgctcg
    10321 gggcactcgc gcaccgcctg ctcgacccac tggttgatac gcaggcagtc ggcttcctcg
    10381 ctggcgccgg ggaagtcttg cagcggcgga tggatggtca ggcgatagcc ggagccatcg
    10441 gccaggcgct gctgggtgaa agggatcacg cgcgccttgc ccaggcgggc gaacttgctg
    10501 gtggcggtga cggtggcggc ggggatgccg aacagcggca cgaaaatgct ctgcttgcgg
    10561 ccatagtcct ggtccggcgc gtaccagatg gcatgcccgg cgcgcagcac cttgagcatg
    10621 gcgcgcacgt catcgcgctc gatggcgctg gcgtcgaggt tgtgccgctc gcgaccgtgg
    10681 cgctggatgt agtcgaacag cgggttcttg tgctcgcggt acatgccgtc gatggtgtgg
    10741 cgctggccga gcagcgcggc accgatttcc agggtggtga agtgcaggga catcaggatc
    10801 accccctgcc cctcgcgcag ggcggcctgc aggtgctcca gcccctcgac atgcgccagg
    10861 cgcgccaggc gggcacgcgg ccaccaccag ctcatcgcca tctcgaagaa ggcgatgccg
    10921 ctggaggcga agttctcgcg cagcaggtgc gcacgctcgc tggcggagag ttcggggaag
    10981 cacagttcga ggttgcgtgc agcgatgtgc cggcgcgacc gggcgcccag cagcatcagc
    11041 acaccgaggg cttgcccgag cttcagcagc gtcttgtagg gcagctgcac gaccaaccag
    11101 agcagcccca gccccagcca cagcagccag aagcgcgggt gaagaaaggc ggcacgaaag
    11161 cggggacgtt ccatcaacac atccagtcag gcgaacaggc cgattattct accccgtcac
    11221 gcacaggcga atcagccatg catgcaactt gcgaccccga tgggttgtcg ttataagtcc
    11281 tcggcatatt cctggctgca accgacatga acctagctga tctgaccgac caagaccccg
    11341 tattccggct caagggcaac atgctcgcga tcaccgtgct ggagcttgcc cgcaacgacc
    11401 tggcactcct cgaccggcag ctggccgaga aggtcgccaa ggcgcccaac ttcttccacg
    11461 acgcgcccgt gctgctcggc ctcgagcatc tcgccgagac ggatgccgag ctggatctcg
    11521 gcagcctgct cgatatctgc caccgccatg gcctgctggc cctcggcgcg cgcgccagcc
    11581 gtcccggcga catcgcggcg gccgaagagc tgggcctgcc gttgctgccc cc
//
LOCUS       DATCUN010000117        17128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_211560, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000117 DATCUN010000000
VERSION     DATCUN010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 17128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17128
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211560"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VIO83_04580"
     CDS             complement(<1..328)
                     /locus_tag="VIO83_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2076 family protein"
                     /protein_id="HEY8330586.1"
                     /translation="MHAEEKNLIDGLFSRLRDAESQSAPRESAAEALINGHIARQPAA
                     PYYMAQAIIVQEQAIQQLDGRVKELEAQIETLKRSQQSAPQQSSGGFLSGLFGGGGRS
                     QPQQPAT"
     gene            647..991
                     /locus_tag="VIO83_04585"
     CDS             647..991
                     /locus_tag="VIO83_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HEY8330587.1"
                     /translation="MSTLPPCPKCGSEYTYEDGAQLVCPECAHEWSADAAAEAPAEGD
                     KVIRDSVGNVLQDGDTVSVIKDLKVKGSSLVVKVGTKVKGIRLCDGDHDIDCKIDGIG
                     AMKLKSEFVKKV"
     gene            1117..2130
                     /locus_tag="VIO83_04590"
     CDS             1117..2130
                     /locus_tag="VIO83_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8330588.1"
                     /translation="MFFRARKSQPINLALQGGGAHGAFTWGVLDALLEDGRVDFEGIS
                     GTSAGAMNAVVLAHGLLQGGREGARQALAHFWEAVAHSAPLISAGADVESGSLSPSAK
                     VMLYWIQQLSPQQFNPLDINPLRDIVSTQIDFEHLRASPLQLFIAATNANTGQLRLFH
                     THELSVASVLASACLPSLHHAIEIDGAPYWDGAFAANPAIFPLLYDCASADILLVLLS
                     PPVQGTTPHSPEEIRRRSQDLAFQSTFLREMRSIARAREFAGRSLLPLGRLERRLMRA
                     NLHLIAMGELTRRLASESKLTTSLPFLEMLRDQGRACTRDWLAAHHQDVGRRSTVNIG
                     DLF"
     gene            2221..2613
                     /locus_tag="VIO83_04595"
     CDS             2221..2613
                     /locus_tag="VIO83_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HEY8330589.1"
                     /translation="MGLFSKILAKLGFGEEAQAAPVDPSSPPTATPTTPTPAAPAGMA
                     MVDVVAKLEGLAAANPEKLNWKTSIVDLLKLLGMDSSLTARKELASELGCPQEKMADS
                     AQMNMWLHKTVLQKLADNGGNVPAELLD"
     gene            2641..3054
                     /locus_tag="VIO83_04600"
     CDS             2641..3054
                     /locus_tag="VIO83_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8330590.1"
                     /translation="MHETRDIHCELLAPLLQPLLGKFYRAQRSPMRAPNGAQTWVARR
                     QEILAALNLSPVGGGHWLTGLLVAPAQRRQGLASRLIEHALAQTPGPVWLFCHPELAD
                     FYARLNFTPAAELPAPLAERLARYRQHKTLVALVR"
     gene            3476..3712
                     /locus_tag="VIO83_04605"
     CDS             3476..3712
                     /locus_tag="VIO83_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330591.1"
                     /translation="MTSKPIAPEADCAEQPQQDQASEATQAAVPAFRFPFTAETYAPK
                     KNGEQPRHPGNNKSNHDKRPGAAPRGTRKSMGKR"
     gene            3821..4213
                     /locus_tag="VIO83_04610"
     CDS             3821..4213
                     /locus_tag="VIO83_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEY8330592.1"
                     /translation="MNTPYQLLGGEDGVRRLCDAFYDCMDSLPEAADIRRMHGEDLSG
                     IRQKLFEYLSGWLGGPHLYAQKYGSICMTGPHRPYAIGPKERDQWLLCMDRALEQVGA
                     SDEVKAMLKGPLQGIADMIRNRETSEPA"
     gene            4486..4827
                     /locus_tag="VIO83_04615"
     CDS             4486..4827
                     /locus_tag="VIO83_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330593.1"
                     /translation="MNTLKLTTLGILLAALGGYAGTALAGNGIQGTFDMTTEAMGVKV
                     RSTTVELTPEFIREGDSKLAIATWEHKDGYVTARDKNGGTLLHARIEDGGDTLIQEIE
                     GIGTATFTRID"
     gene            5024..5545
                     /gene="msrB"
                     /locus_tag="VIO83_04620"
     CDS             5024..5545
                     /gene="msrB"
                     /locus_tag="VIO83_04620"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019363500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY8330594.1"
                     /translation="MNRRILLQALACLPALALLEFGASPARAAAPDTVTVTPLDKPPA
                     AWRAVLTPAAYRVLFEGDTEPSGSSPLDDEHRDGTFVCAACYLPLFDSRHKYDSGTGW
                     PSFTQPITGHVGRKRDFRLIWPRTEYHCARCGGHQGHLFDDGPPPRGERWCNNGLALR
                     FVPRADPLPELRN"
     gene            5548..6210
                     /gene="msrA"
                     /locus_tag="VIO83_04625"
     CDS             5548..6210
                     /gene="msrA"
                     /locus_tag="VIO83_04625"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY8330595.1"
                     /translation="MSRAIAYPPLPDGLRRGLLALLLAASTLTGLPHADAAESGGTTD
                     DQAATAIFAGGCFWCMESDFDKVPGVLSTTSGYTGGSLANPTYEQVSAGRSGHVEALL
                     VRFDPTQTSYAQLLDAYWPNIDPLTATGQFCDIGSQYRSAIFYRTPEQQRLAEASKVA
                     LQASGHFTKPIVTEILPAGTFYPAEEYHQDYYRKNPLRYFYYRTTCGRDARLETLWGK
                     RP"
     gene            complement(6274..7110)
                     /locus_tag="VIO83_04630"
     CDS             complement(6274..7110)
                     /locus_tag="VIO83_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HEY8330596.1"
                     /translation="MALIGRFNSLQVVKHTDFGLYLDGGPDGEILLPKRYVPKGEPCE
                     IGDWLNVFVYLDSEDRIIATTQTPKVQVGGFASLKVVEISHVGLFLDWGLPKDLFLPH
                     SEEKRPLQVGDYCVVHVYLDARTRRITATARLDRYLDKTPPNYTPGQAVELLVVERTD
                     LGFKAIVNGKHWGLIHKNELFKFLRPGMQESGYIREVRADGKISLSLQPIGKVAADSL
                     GEQILAKLREEGGSLALSDKSPPEVISRLFGVSKGNFKKAIGALYKQGLIVILDERIE
                     LR"
     gene            7520..7729
                     /locus_tag="VIO83_04635"
     CDS             7520..7729
                     /locus_tag="VIO83_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY8330597.1"
                     /translation="MSNRQTGTVKWFNDEKGFGFITPQSGPDVFVHFRAIKADGFKSL
                     KEGQAVSFLVVQGQKGLQADEVQIV"
     gene            8042..8227
                     /locus_tag="VIO83_04640"
     CDS             8042..8227
                     /locus_tag="VIO83_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330598.1"
                     /translation="MQSKPSASKQKVAPVPSAEARLALEEQMAAFLKSGGEVQQIPRG
                     VSGQVYGSSRQITIGKK"
     gene            8232..8942
                     /locus_tag="VIO83_04645"
     CDS             8232..8942
                     /locus_tag="VIO83_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEY8330599.1"
                     /translation="MSDPVRLSKRLAELLPCSRREAELYIEGGWVTVDGQVVEEPQFK
                     VQAQTIALREGAKAEPQPPITLILHKPADLDSEHACELLSAASRAADDASGVHLLQRH
                     LQRLSVPLPLEPEASGLLVLTQTWGVIRKLTDDFSKIEQEFVVDVVGTLTPEQLKQLQ
                     FGLSYRGRPLTPCKVSWQSETRLRFALKAPQPGQIAHVCRTVGLQVLSIRRIRIGRVA
                     MAKLQPGQWRYLGGNERF"
     gene            complement(9028..10176)
                     /locus_tag="VIO83_04650"
     CDS             complement(9028..10176)
                     /locus_tag="VIO83_04650"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8330600.1"
                     /translation="MRPLSLLRGCALLLCVGLLQAAQAAPALGSAHGPLELHELVGGL
                     EHPWALAFLPDGQGMLVTERPGRLRRVSAEGALSAPLAGVPAVYAQGQGGLLDIALSP
                     QFAADRLVYLSYAEAGPDGYAGTAVGRGRLSADLRALEDFQVIFRQQPKLSSGTHFGS
                     RLVFDRAGYLFVTLGENNQRPTAQDLGKLQGKVVRLHADGRIPADNPLVGKAGARGEI
                     WSYGHRNPQGAALNPWTGQLWVHEHGPRGGDEINLPRPGRNYGWPLATHGINYSLLPI
                     PEAQGDRAPGTEAPWYVWKRSPAISGMAFYDGARFPAWRHSLFVGALAGQALIRLTLD
                     GDRVAGEERLLESLNARIRDVRTGPDGWVYVLTDERDGKLLRVGLAQP"
     gene            complement(10173..10562)
                     /locus_tag="VIO83_04655"
     CDS             complement(10173..10562)
                     /locus_tag="VIO83_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEY8330601.1"
                     /translation="MIVCKRVYLPAMPEDGFRVLVDRLWPRGCSKASLVLDAWLRELA
                     PSDALRQAFAHDPGHFAAFRRAYRAELATLPAQWRPLLERARHGTLTLLYAARDEQLN
                     NARVLAEFLDEQLALPAPADCAAGASR"
     gene            complement(10614..10934)
                     /locus_tag="VIO83_04660"
     CDS             complement(10614..10934)
                     /locus_tag="VIO83_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330602.1"
                     /translation="MSERNPIPLILTAVGSVAATVALLGYYGYLHFAKPEDALLLSEY
                     TMLKTVPGEDYKVSLKPADQVAHCIDGVLVLFDVNQKGLTGVLVDSQKRAVRCMGEET
                     PQAE"
     gene            complement(10989..12281)
                     /locus_tag="VIO83_04665"
     CDS             complement(10989..12281)
                     /locus_tag="VIO83_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavohemoglobin expression-modulating QEGLA
                     motif protein"
                     /protein_id="HEY8330603.1"
                     /translation="MSRSKANTEYRQTIRALSDRLVEAQTPIRVLDAIKWDDGIREGF
                     LKSRGSKPPAVDRAYYQNRPLSFDADQKKQQFQQIERDITRQLGQFSPVGQIMRRMCK
                     EYRMVIRMLEARGTADFGLISQELYGSASDAFHAGDPTLADLGVMFSTYLDNIAGRSE
                     LKDEPKTLNATEAVALLQGQLNGVFGDEAVVRVFESDGILADAAAGADYIKIRADALF
                     NQRDIRALAVHEGLVHVGTTLNGLNQPVCTFLAKGPPSSTITQEGLAILMEVIAFTSY
                     PTRLRKLTNRTRAIHMAEEGADFLQIFEFYRDQGYTAEEGYSNTIRVFRGSTPDGLPF
                     TKDLSYLKGFILIYNYIQLAVRKGKLEQIPLLFCGKATLEDMRTLRQLVDEGLVVPPK
                     YLPPQFSDLNALSAWMCFSNFLNHLSLDRVEADYANIL"
     gene            complement(12294..12845)
                     /locus_tag="VIO83_04670"
     CDS             complement(12294..12845)
                     /locus_tag="VIO83_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330604.1"
                     /translation="MNAVRVLVVLSFLLLGGCLATFKEPIPAGETAPKHLLGTWAGED
                     EWGEERFLEITRKPGGGYQALAWRESMANRDKARGYDFTVAHHGRRWYLSVEAPKRLG
                     GNFAIGGFELTDEDELVLYSLDIEQLQGALASGQLEGQTVETDEGDGVLIASPLAKVY
                     RFLDDPANSDVFSEVARFVRVTE"
     gene            complement(12982..14487)
                     /gene="lysS"
                     /locus_tag="VIO83_04675"
     CDS             complement(12982..14487)
                     /gene="lysS"
                     /locus_tag="VIO83_04675"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY8330605.1"
                     /translation="MSEQPLNEHALHEEENKLIAQRKEKLAALRESQAIPFPNDFRRD
                     SQAGDLQKQYAEAGKEALEAQQIKVSVAGRIMLNRGAFMVIQDTSGRIQLYVNRKTLS
                     AETLEAIKHWDLGDIVGVSGVLARSGKGDLYVDMQDVRLLTKSLRPLPDKFHGLTDTE
                     TRYRQRYVDLIVNEEVRDTFRVRSQVIAHIRRFLNDRGFLEVETPMLQTIPGGAAAKP
                     FETHHNALDMPMFLRIAPELYLKRLVVGGFEKVFEINRNFRNEGVSTRHNPEFTMLEF
                     YHAYADYEDNMDLTEELFRELAQTVLGSTDVPYGDKLFHFGEPFARLSVFDSILKYNP
                     EISAADLQDLDTARAIAKKAGAKVLGFEGLGKLQVMIFEELVEHKLEQPTFITEYPFE
                     VSPLARRNDKNPNVTDRFELFIGGREIANAYSELNDAEDQAERFHAQVRDKDAGDDEA
                     MHFDADFVRALEYGMPPTAGEGIGIDRLVMLLTNAPSIRDVILFPHMRAEQ"
     gene            complement(14618..15713)
                     /gene="prfB"
                     /locus_tag="VIO83_04680"
     CDS             complement(join(14618..15640,15642..15713))
                     /gene="prfB"
                     /locus_tag="VIO83_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933612.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY8330606.1"
                     /translation="MEINPIVNSIKDLTSRTQTIRGYLDYDQKHDRLTEVNRELEDPN
                     VWNNPEYAQNLGRERAQLAQIVETLDELASGLADSSDLLAMAVEENDEGAVNDVATEV
                     ERLREILEKLEFRRMFSGEMDANNAYLDIQAGSGGTEAQDWANMLLRMYLRWADKHGF
                     EATIMELSAGEVAGIKGATVHIKGEYAFGWLRTEIGVHRLVRKSPFDSGNRRHTSFTA
                     VFASPEIDDNIEIDINPADLRTDTYRSSGAGGQHVNTTDSAVRITHIPTNTVVSCQNE
                     RSQHANRDTAMKMLRAKLYELEVQKRNAAAQALEDTKSDIGWGHQIRSYVLDQSRIKD
                     LRTGVEESNCDAVLDGDLDEFIEASLKQGL"
     gene            complement(15887..>17128)
                     /locus_tag="VIO83_04685"
     CDS             complement(15887..>17128)
                     /locus_tag="VIO83_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY8330607.1"
                     /translation="GLMIGDTVIIRRAGDVIPQVMQVVLERRPADARPVAIPQSCPVC
                     GSHVERTQLIKRGKGKASVSEGSIYRCVGRLSCQAQLKQAIIHFVSRRAMDIDGLGDK
                     IVEQLVDVGLVRSPADLYTLTFEQVVVLEGFAELSTRNLLAAIDASKRPTLARFIYAL
                     GIPDVGEETAKLLARALGALARIAVALPEVLTWLPDVGLEVAHEIHSFFEDEHNRTVI
                     AQLLARGVVLQEEGALHAEFAACATLAELLNKLNIAGIAATGAKNLADAAGSLEGVIA
                     LSQDWLSLSVTKGLSEKARQALREHFAAPANVAHALAIEAQLREFGMHWQSEKKAVAG
                     LPLAGQTWVLTGTLEAMSRDEGKARLEALGAKVAGSVSAKTHCVVAGPGAGSKLTKAS
                     ELGVAVLDEAAFLARLAELGG"
BASE COUNT         2946 a         5804 c         5508 g         2870 t
ORIGIN      
        1 tagtggcggg ctgctgcggc tggctacggc caccaccgcc gaacagcccg gagaggaagc
       61 cgccgctgct ctgctgcggc gcgctctgct ggctgcgctt gagggtttcg atctgcgctt
      121 ccagttcctt gacgcgacca tccagctgct ggatggcctg ttcctgcacg atgatcgcct
      181 gggccatgta gtagggcgcc gccggctggc gggcgatgtg cccgttgatc agggcttcgg
      241 ccgcggactc gcggggcgcg gactggcttt cggcgtcgcg caggcgggaa aacaggccat
      301 cgatcaggtt tttctcttca gcgtgcatgc ttgtctcttc agtgggttgc cgcaagaagc
      361 ccatcttcgg aacccgcacg ggggatgtca aaatccagct attgcaagag ttgtcatcgc
      421 cgccgcggag ctggccgcgc gcacgggcgc cccgggcggc cagcgcccat gccccggcag
      481 tcgacagcca catgaccgat catcatgcgc caggagtgcc tggcaggctg gcgaatcatc
      541 cgccgcctgc cccccaactc gcgctttctg ccgtgcggcg ctcctcggag ggagcaaaac
      601 cttatataat tcgcggactt ttaccgatcg aacatgagga taattcatga gcactctgcc
      661 accctgcccc aaatgcggtt ccgaatacac ctacgaggac ggcgcgcagc tggtctgccc
      721 ggaatgcgcc catgagtggt ccgccgatgc cgccgccgaa gccccggccg aaggcgacaa
      781 ggtgatccgc gactcggtcg gcaacgtcct gcaggatggc gacaccgttt ccgtgatcaa
      841 ggacctcaag gtcaagggct cgtcgctggt ggtcaaggtc ggcaccaagg tcaagggcat
      901 ccgcctgtgc gacggcgacc atgacatcga ctgcaagatc gacggcatcg gcgcgatgaa
      961 gctgaagtcg gagttcgtca agaaggtctg aacctgagcg ctcccgccac tgcggcggga
     1021 gcacgacgcg ccgaccggcg ccgctgacac acgcgtgaca ctggaatggg ttttgctgag
     1081 caaatgccat tcccggtcca accgcagggt cacgccatgt tctttcgcgc gcgcaagtcc
     1141 cagcccatca acctcgccct gcagggcggt ggtgcgcatg gggcctttac ctggggcgtg
     1201 ctggatgccc tgcttgagga cggccgcgtc gacttcgagg gcatcagcgg caccagcgcg
     1261 ggagcgatga acgccgtggt gctcgcccac ggcctgctgc agggcggccg cgagggtgcg
     1321 cgccaggcac tggcgcactt ctgggaggcg gttgcgcaca gcgcacccct gatcagcgct
     1381 ggtgccgatg tcgagagtgg cagcctgtcg cccagcgcca aggtgatgct gtactggatc
     1441 cagcaactgt cgccgcagca gttcaacccg ctcgacatca atccgctgcg cgacatcgtc
     1501 agcacgcaga tcgacttcga gcacctgcgc gccagcccgc tgcagctgtt catcgccgcc
     1561 accaacgcca ataccggcca gctgcgcctg ttccacaccc atgaactgag cgtggcgagc
     1621 gtgctggctt cggcctgcct gcccagcctg caccacgcca tcgagatcga cggtgcgccc
     1681 tactgggatg gcgcctttgc cgccaatccg gcgattttcc cgctgctcta tgactgcgct
     1741 agcgccgaca tcctgctggt gctgctaagc ccgccggtgc agggcaccac gccgcacagc
     1801 cccgaggaga ttcgccgccg ctcgcaggat ctggcgttcc agtccacctt cctgcgcgag
     1861 atgcgcagta tcgcccgcgc acgggagttc gccgggcgct cgctgctgcc gctcgggcgc
     1921 ctggagcgac gactgatgcg ggcgaacctc catctgatcg ccatgggcga gctgacccgg
     1981 cggctggcca gcgagtccaa gctgaccacc agcctgccct ttctggagat gctgcgcgac
     2041 cagggccggg cgtgcacccg cgactggctc gccgcccacc accaggatgt cggccggcgt
     2101 tcgacggtga atatcggcga cctgttctga atttcgtcgc ccggcacgcc ccatcgtgcg
     2161 gcaacgcctg ccgtgtgcgg ctatcctccc ttgcgccact cactcggtag aggaattccc
     2221 atgggactgt tcagcaagat cctggccaag ctcggctttg gcgaagaggc acaggccgcg
     2281 ccggtggatc catcaagtcc gccgaccgcg acgcccacca ccccgacgcc ggcagcgcca
     2341 gcgggcatgg ccatggtcga cgtggtcgcc aaactggaag ggctggccgc cgccaatccg
     2401 gaaaagctca actggaagac ctcgatcgtc gacctgctca agctgctcgg catggacagc
     2461 agcctgaccg cccgcaagga gctcgccagc gaactgggct gcccgcagga gaagatggcc
     2521 gattcggcgc agatgaacat gtggctgcac aagaccgtgc tgcagaaact ggctgacaac
     2581 ggcggcaacg tgccggccga gctgctcgac tgaaccgcag cgccagacgc gatcacccgc
     2641 atgcacgaaa cacgcgatat ccactgcgag ctgctcgcgc cgctgttgca gcccctgctg
     2701 ggcaagttct accgcgccca gcgctcgccc atgcgcgctc ccaatggcgc gcagacatgg
     2761 gtggcgcggc gccaggaaat cctcgctgca ctgaacctga gcccggtggg cggcggtcac
     2821 tggctgaccg gcctgctggt ggcgcccgcg cagcggcgcc agggcctggc cagccgattg
     2881 atcgagcatg ccctggccca gacgccggga ccggtgtggc tgttctgcca tccggagctt
     2941 gccgacttct acgcacgcct gaacttcacc cccgcggcgg agctgcctgc cccgctggcg
     3001 gagcgcctgg cacgctaccg acaacacaag acgctggtcg cgctggtccg ctaagcggcc
     3061 acgcaactac cgcggcctga gcaaggtcgg cggagtcggc attattaacg cggggagagg
     3121 ctacgccttg ggcgggcgca tggcgcagcg ggatcgcggc accgggcagg agaaccggcc
     3181 gcccgggacc gggcgacagg cctgcaacgt cagacttcga gggagtcgcc gacgtcgacc
     3241 agcttgcggc gggccagggc gccttctacg gtcatcagtt gatcaaggcc gtctttgata
     3301 gctgtcattc ttattactca caggttactg gccgggcgct cgcccagctc gttcagggat
     3361 tcgctcagcc atctggccaa gcgccgatgc ccgggcatct atgcaggtat aatgcgcagc
     3421 acgtgcgccg atgcttggcg atgtagaacc tctcaaccgc tattccggat acaccatgac
     3481 atcgaaaccc attgcccccg aggccgactg cgccgagcaa ccgcaacagg accaggccag
     3541 cgaagctacc caggccgctg ttccggcttt ccgcttcccc ttcacggccg agacctacgc
     3601 gccgaagaag aacggcgagc agccccggca cccaggcaac aacaagagca atcacgacaa
     3661 gcgcccgggc gcggctccca ggggcacccg caagtcgatg ggcaagcgct gaaaccagcg
     3721 ccgcccccac cggcaggaag ccgacttgct gacctggatc aagccctggc caccgcactc
     3781 gggcagcatc aatgcttttc gcaacacgac ggagagccgc atgaacactc cctatcaact
     3841 gttgggcgga gaggatggcg tacgccgtct gtgcgacgct ttctacgact gcatggacag
     3901 cctgcccgaa gccgcggaca ttcgccgcat gcacggcgag gatctctccg ggattcgcca
     3961 gaagctgttc gaatacctgt ccggctggct gggcggcccg cacctgtatg cgcagaagta
     4021 cggctcgatc tgcatgaccg gcccgcaccg cccttacgcc atcggcccga aggagcgtga
     4081 ccagtggctg ctgtgcatgg atcgcgcact ggagcaggtc ggcgccagcg atgaagtgaa
     4141 ggccatgctc aaggggccgc tgcaggggat cgccgacatg atccgcaacc gcgaaacctc
     4201 cgaaccggcc tgaaacgcca gcagccgggc ctcgccgcgc gaggcccggc acgcccgcaa
     4261 aacccgcact gtcacgataa gttcatgaaa ttgcaggctt tttgacgatc caggtcaata
     4321 aaaccctgct tcgcgcctcc taagctgatc ccgcatcgca gcactgccgc ccggcacgcc
     4381 ggacgcgcaa tgccacgcac cccgcatcgc tcagccatca cgttgaccgt ctgcgctccg
     4441 cgcaggaaag cgacggttca atgtcatcga caaggaaggc acgccatgaa cacgctcaag
     4501 ctcaccaccc tcggcatcct gctggccgca ctgggcggtt acgccggcac ggccctggct
     4561 ggcaatggca tccagggcac cttcgacatg accaccgagg ccatgggcgt gaaggtccga
     4621 agcaccaccg tcgaactgac gccggagttc atccgtgagg gcgacagcaa gctggccatc
     4681 gccacctggg agcacaagga cggctacgtc accgcccgtg acaagaacgg cggcacgctg
     4741 ctgcacgccc gcatcgagga tggcggcgac accctgatcc aggagatcga gggcatcggc
     4801 accgccacct tcacccgcat cgactgaggc cgcatcggtc gcgccgcccg tgtgcagatc
     4861 gcggaactgg cgcgggatcg cgttgctctg atcccgcgcc gcgtgtgcac ggaacaaccg
     4921 tatgtcgcct gacggcaaaa tccatcacca ccagtagctg taccggcaac gcctgagggc
     4981 gggagctgtc caatcttcgc ataccctttc ccgggagccg gcgatgaaca gacgcatcct
     5041 gctgcaggcc ttggcctgcc tgccagcctt ggcactgctg gagttcggcg catcccctgc
     5101 gcgggcagcc gcgccggaca ccgtaaccgt gaccccgctc gacaagccgc ccgccgcgtg
     5161 gcgcgccgtg ctgacgcccg cggcctaccg agtgctgttc gagggcgaca ccgaaccgtc
     5221 cggaagcagc ccactcgacg acgaacaccg cgacggcacc ttcgtctgcg ccgcctgcta
     5281 cctgccactg ttcgacagcc ggcacaagta cgacagcggc accggctggc ccagcttcac
     5341 ccagccgatc accggacacg tcgggcgcaa gcgcgacttc cgcctgatct ggccgcggac
     5401 cgaatatcac tgcgcgcgct gcggcggaca ccagggccac ctgttcgacg acggcccgcc
     5461 gccacgcggc gagcgctggt gcaacaacgg cctggcactg cgcttcgtgc cgcgcgccga
     5521 tcccctgccc gaactgagga actgatgatg agccgcgcaa ttgcgtatcc gccgctgccg
     5581 gacggcctgc gccgcggatt gctggcgctg ctgctggccg ccagcaccct gaccggcctg
     5641 ccgcacgccg acgcggcgga gagcggcggg acgaccgacg accaggccgc cacggcgatc
     5701 ttcgccggcg gctgcttctg gtgcatggag tcagacttcg acaaggtgcc gggcgtgctg
     5761 tccaccacct ccggctatac cggcggtagc ctggccaatc cgacctacga acaggtgtcg
     5821 gcgggacgca gcggccatgt cgaggcgctg ctggtgcgct tcgatccgac gcagaccagc
     5881 tatgcccagc tgctggacgc ctactggccg aatatcgacc cgctgaccgc caccgggcag
     5941 ttctgtgaca ttggctcgca gtaccgcagc gcgatcttct accgcacgcc cgagcagcaa
     6001 cgcctggccg aagcgtcgaa agtggcgcta caggcttccg ggcactttac caagccgatc
     6061 gtcaccgaga tcctgcctgc cgggaccttc tatcccgccg aggagtatca ccaggactac
     6121 taccggaaga acccgctgcg ctatttctac taccgcacca cctgcggtcg cgatgcgcgc
     6181 ctggaaacgc tatggggcaa gcgcccctga ctccactcag ggcgggacgt tgccggcacg
     6241 cggacagctg ccccgtgccg ggcaaccttc ggctcagcgc agctcgatgc gctcgtcgag
     6301 gatgacgatc agcccctgct tgtacagggc gccgatggcc ttcttgaagt tgcccttgct
     6361 cacgccgaac agccggctga tcacttccgg cgggctcttg tcgctcagtg ccaggctgcc
     6421 gccctcctcg cgcagcttgg cgaggatctg ttcgccgagg ctgtcggccg ccaccttgcc
     6481 gatcggctgc aggctcaggc tgatcttgcc gtccgcacgc acctcgcgga tgtaaccgct
     6541 ttcctgcatc cccggacgga ggaacttgaa cagctcattc ttgtggatca ggccccagtg
     6601 cttgccgttg acgatggcct tgaaaccgag gtcggtacgc tcgaccacca gcagctcgac
     6661 ggcctgcccc ggcgtgtagt tgggcggagt cttgtccaaa tagcggtcca gccgcgcggt
     6721 ggccgtgatg cgccgggtgc gcgcatcgag atagacatgc actacgcagt agtcgccgac
     6781 ctgcagcgga cgtttttcct cggagtgcgg caggaacagg tccttgggca gcccccagtc
     6841 gaggaacagg ccgacatgac tgatctccac caccttgagg ctggcgaaac cgccgacctg
     6901 caccttgggc gtctgcgtgg tggcgatgat gcggtcttcg ctgtccagat agacgaacac
     6961 gttaagccag tcgccgatct cgcacggctc gcccttgggc acatagcgtt tgggcagaag
     7021 gatttcgccg tccggaccgc cgtccagata gaggccgaag tcggtgtgct tgaccacctg
     7081 cagagagttg aaccgcccga tcagtgccat gtcgcttacc ttggattgcc agaggcagca
     7141 ttctagccga gatcggccgg cgaattgcgg gcgcaagcgg ggcaatcggt ggcagatcgc
     7201 gcggctttcc gacgggcgct gcggagcgcc gtcagaccgc accaagatag ctatcttgtt
     7261 gattaaaaag atgatttatt ggctgccgcg gcccccgaag gaaaacccga ctccacaccg
     7321 gcacaggggg cccaaaagcc gcccatagaa agaatttgca ccggacgaaa ttaccgttta
     7381 ggttatcgat cactgtgctg cagcgtcatg gtttagatct tgattcattc aaatttcagc
     7441 tttcctggct ccttgcactc agttccgacc ccgggcagtt ccggtgtcgc agtcaaaact
     7501 gttgctcaag gagactttta tgtctaatcg ccaaaccggt accgtcaagt ggttcaacga
     7561 tgagaaaggc ttcggcttca tcaccccgca gagcggtccg gacgtgttcg ttcacttccg
     7621 cgccatcaag gccgacggct tcaagagcct gaaggaaggc caggctgttt ccttcctggt
     7681 tgtccagggc cagaaaggcc tgcaggctga cgaagtccag atcgtctgat ctgactttcc
     7741 agtctgaaca aaagccccgc ctcgtgcggg gcttttgcat ttccagggcc tagccaaatg
     7801 ccccatccgc tcctgcctgc gaggtgccgc catgccgctg aagctgacca tcgtcgacca
     7861 gacgcccgtc cacggcgacc gcccggccag cgagtgagcc ggcgggggaa aatggccatt
     7921 tcccgacttt ccccagcctt tttccgacag atactttcct gatttcatca tcctgcgcaa
     7981 aaaatgaccg ccaatgctgt acaatagtga gcctttttcc tcttcaggca ggagaatcac
     8041 catgcaaagc aaacccagcg cctccaaaca gaaggttgcc cccgttccct ccgccgaagc
     8101 ccgcctcgcg ctcgaagagc agatggcagc cttcctcaag tccggcggcg aagtccagca
     8161 gatcccccgt ggcgtcagcg gtcaggtcta cggcagctcg cgccagatca ccattggcaa
     8221 gaagtgattc gatgagcgac ccggtccgtc tgtccaaacg cctcgccgaa ctgctgccct
     8281 gctcgcgccg cgaagccgag ctgtatatcg agggcggctg ggtcacggta gatggccagg
     8341 tggtcgagga gccgcagttc aaggtgcagg cgcagaccat cgccctgcgc gaaggcgcca
     8401 aggcggaacc ccagccgccg atcaccctga tcctgcacaa gccggccgac ctggacagcg
     8461 aacatgcctg cgagctgctg agcgctgcca gccgcgccgc ggacgacgcc tctggcgtgc
     8521 acctgctgca gcgtcacctg cagcgtctga gcgtgccgct gccgctggag cccgaggctt
     8581 ccggcctgct ggtcctgacc cagacctggg gcgtgatccg caagctgacc gacgacttca
     8641 gcaagatcga gcaggaattc gtcgtcgatg tcgtcggcac gctgacgccg gagcagctca
     8701 agcagctgca gttcggcctc agctaccggg gccgcccgct gacaccgtgc aaggtcagct
     8761 ggcagagcga aacccgcctg cgctttgccc tcaaggcgcc gcagccgggg cagatagccc
     8821 acgtctgccg taccgtcggc ctgcaggtgc tcagcatccg ccgcatccgc atcggccgcg
     8881 tggccatggc caagctgcaa cccggccagt ggcgctacct cggcggcaac gaacggttct
     8941 gaccccgacg cctgcagggt ggaaactcgc ccagcgagtt cccaccggca acggcggcca
     9001 agtctgcgcc gttgaccagc cgtggcatca cggctgcgcc agccccaccc gcagcagctt
     9061 gccgtcgcgc tcgtcggtga gcacatacac ccagccatcc ggccccgtcc gcacgtcgcg
     9121 gatgcgcgca ttcagcgact ccagcagacg ctcctcaccc gccaccctgt cgccgtccag
     9181 ggtcaggcgg atcaacgcct ggccagccag tgcaccaacg aacaggctgt ggcgccaagc
     9241 cggaaaacgc gcgccgtcat agaaggccat gccgctgatc gctggcgacc ttttccagac
     9301 gtaccagggc gcttcggtgc cgggcgccct gtcgccctgc gcctcgggaa tcggcagcag
     9361 gctgtagttg atgccatggg tggccagcgg ccagccatag ttgcgccccg gccggggaag
     9421 gttgatttcg tcgccaccgc gcgggccgtg ctcgtggacc cacagctgcc ccgtccacgg
     9481 attgagcgcc gcgccctgcg ggttgcgatg accgtaggac cagatttccc cacgcgcacc
     9541 ggccttgccg accaagggat tgtccgcggg aatgcggcca tccgcgtgca ggcgcaccac
     9601 cttgccctgc agcttgccga ggtcctgggc cgtggggcgc tggttgttct cgcccagcgt
     9661 gacgaacaga tagccggccc ggtcgaacac cagccgcgaa ccgaaatgcg tgccggagga
     9721 gagcttgggc tgctggcgga agatcacctg gaaatcctcc agcgcgcgca ggtcggcaga
     9781 cagccgcccc cgcccgaccg ccgtaccggc gtaaccgtcc ggccccgcct cggcatagct
     9841 caggtagacc aggcgatcgg cggcgaactg cggcgacagg gcgatatcca gcaggccgcc
     9901 ctgcccctgg gcgtagaccg caggaacccc ggccaacggc gccgacagcg cgccctcggc
     9961 agacacccgg cgcagacgtc ctgggcgctc ggtcaccagc atgccctgcc cgtccggcag
    10021 gaaagccagt gcccagggat gctccagccc gccgaccagc tcgtgcagtt ccagcgggcc
    10081 gtgcgcgctg cccagcgctg gcgcggcctg cgccgcctgc agcaggccga cgcacagcag
    10141 gagcgcacag ccgcgcagca aggacagggg cctcatcggg atgctcccgc ggcgcaatcg
    10201 gccggcgccg gcaaggccaa ctgttcgtcg agaaactcgg ccagcacccg ggcgttgttc
    10261 aactgctcgt cgcgcgcggc atacagcagg gtcagcgtgc cgtggcgagc ccgctccagc
    10321 agcgggcgcc actgcgccgg gagcgtcgcc agctcggcac ggtaggcgcg gcgaaatgcg
    10381 gcgaagtgcc cgggatcatg ggcgaaggcc tggcgcagcg catccgacgg cgccagctcg
    10441 cgcagccagg catccagcac caggctggcc ttgctgcagc cgcgcggcca caaccgatcg
    10501 accagcacgc ggaaaccgtc ttccggcatg gctggcagat acacgcgctt gcagacaatc
    10561 atcgtccccg ccctcgcgat cacctcgtcc gggcaaggat agaccgacgg acctcactcg
    10621 gcctgcggcg tctcctcgcc catgcaacgc accgcgcgct tctggctgtc caccagcacg
    10681 ccggtcaggc ccttctggtt gacgtcgaac agcaccagca cgccgtcgat gcagtgggcg
    10741 acttggtcgg cgggcttgag ggaaaccttg tagtcctcgc cggggacggt cttgagcatg
    10801 gtgtattcgc tcagcaacag cgcgtcctcg ggcttggcga agtgcagata gccgtagtag
    10861 ccgagcagcg cgacggtcgc agcgacgctg ccgacggcgg tgaggatcag cggaatgggg
    10921 ttgcgttcgc tcatgcgggt atccggaaag tcagcaaagg ggttgccaga gacgagccct
    10981 gcggcccgtc agaggatgtt ggcgtagtcg gcctcgacac ggtccaggct caggtggttg
    11041 aggaagttgg agaagcacat ccacgccgac agcgcattga gatcgctgaa ctgcggcggc
    11101 aggtacttgg gcggcacgac cagcccctca tccaccagtt ggcgcagcgt gcgcatgtct
    11161 tccagggtcg ccttgccaca gaacagcagc ggaatctgct ccagcttgcc cttgcgcacg
    11221 gcgagctgga tgtagttgta gatcaggatg aagcccttga ggtaggacag gtccttggtg
    11281 aacggcaggc cgtccggggt cgagccgcgg aagacgcgga tggtgttgga gtagccttcc
    11341 tccgccgtgt agccctgatc gcggtagaac tcgaagatct gcaggaagtc cgcgccctcc
    11401 tcggccatgt ggatggcacg ggtgcggttg gtcagcttgc gcagccgggt cggataggag
    11461 gtgaaggcaa tcacctccat gaggatggcc aggccctcct gggtgatggt cgaggacggc
    11521 gggcccttgg ccaggaaggt gcagaccggc tggttcaggc cgttgagggt ggtgccgacg
    11581 tgcaccagcc cctcgtgcac cgccagggcg cggatgtcgc gctggttgaa cagcgcatcg
    11641 gcgcggatct tgatgtagtc ggcgccggcc gcggcatcgg cgaggatgcc gtcggactcg
    11701 aacacccgca ccaccgcctc gtcgccgaac acgccgttga gctggccctg cagcagcgcc
    11761 accgcctcgg tggcattcag cgtcttcggt tcgtccttca gctcgctgcg gccggcgatg
    11821 ttgtccaggt aggtggagaa catcacgccg aggtcggcca gggtcggatc gccggcatgg
    11881 aaggcgtcgg acgccgagcc gtacagctcc tgggagatca gcccgaaatc cgcggtaccg
    11941 cgcgcctcga gcatgcggat gaccatgcgg tattccttgc acatgcgccg catgatctgc
    12001 ccgaccgggc tgaactggcc gagctgacgg gtgatgtcgc gctcgatctg ctggaactgc
    12061 tgcttcttct ggtcggcatc gaaggacagc ggccggttct ggtagtaggc ccgatccacc
    12121 gccggcggct tgctgccgcg ggacttgaga aagccctcgc gaataccgtc gtcccacttg
    12181 atcgcatcga ggacgcggat cggcgtctgc gcctcgacca gccggtcgga cagcgcacga
    12241 atggtctgcc ggtattcggt attcgccttg ctgcgactca tcgccccgcc ccgttattcg
    12301 gtgacccgta cgaagcgggc gacctcgctg aacacgtcgc tgttggccgg gtcgtcgagg
    12361 aagcggtaga ccttggccag cggactggcg atcaacacgc cgtcgccctc gtcggtctcc
    12421 accgtctgcc cctcgagctg gcccgacgcc agggcaccct gcagctgctc gatatccagg
    12481 ctgtagagca ccagctcatc ctcgtcggtc agctcgaagc cgccaatggc gaagttgccg
    12541 cccagacgct tgggcgcttc gaccgacagg taccagcggc ggccgtgatg ggccacggtg
    12601 aaatcatagc cgcgcgcctt gtcgcggttg gccatgctct cgcgccaggc caatgcctgg
    12661 tagccaccgc cgggcttgcg ggtgatctcg aggaaacgct cctcgcccca ctcgtcctcg
    12721 ccggcccagg tgccgagcag atgtttcggc gccgtctcgc ccgccgggat cggctccttg
    12781 aaggtcgcca ggcagccgcc cagcagcagg aaggacagga caacaagcac acgcacggcg
    12841 ttcatggagc actccttatt ccaacggaca ttttccgctg ccggcagggg cagccacggg
    12901 gagcacgcag cgagcggtgc cccttgccgc attcgaccac gacaaggggc acccgactca
    12961 aggtgatttc acagcagcag cttattgctc cgcgcgcatg tgcgggaaca ggatcacgtc
    13021 acgaatcgac ggcgcattgg tcagcagcat gaccaggcgg tcgatgccga tgccttcgcc
    13081 agcggtcggc ggcatgccgt attcgagggc gcgcacgaag tcggcgtcga agtgcatggc
    13141 ctcgtcgtca ccggcatcct tgtcgcgcac ctgggcatgg aagcgctcgg cctggtcttc
    13201 ggcgtcgttc agctcggagt aggcattggc gatctcgcgg ccgccgatga acagctcgaa
    13261 gcgatcggtg acattcgggt tcttgtcgtt gcgacgggcc agcggcgaca cctcgaacgg
    13321 atactcggtg atgaaggtcg gctgctccag cttgtgctcg accagctcct cgaaaatcat
    13381 cacctgcagc ttgcccagcc cttcgaagcc gagcaccttg gcgccggcct tcttggcgat
    13441 ggcgcgggcg gtgtcgaggt cctgcaggtc ggccgcggag atctccgggt tgtacttgag
    13501 gatcgagtcg aacaccgaca ggcgcgcgaa cggctcgccg aagtggaaca gcttgtcgcc
    13561 gtagggcacg tcggtgctgc cgagcacggt ctgcgccagt tcgcggaaca gttcctcggt
    13621 gaggtccatg ttgtcttcgt agtcggcgta ggcgtggtag aactcgagca tggtgaactc
    13681 gggattgtgc cgggtcgaca cgccttcgtt gcggaagttg cggttgatct cgaagacctt
    13741 ctcgaagccg ccgaccacca ggcgcttgag gtacagctcc ggcgcgatgc gcaggaacat
    13801 cggcatgtcc agcgcgttgt ggtgggtctc gaacggcttg gccgccgcgc cgccggggat
    13861 ggtctgcagc atcggggttt ccacttcgag gaaaccgcgg tcgttgagga aacgacggat
    13921 gtgggcaatc acctgggaac gcacgcggaa ggtgtcgcgc acctcctcgt tgacgatcag
    13981 gtcgacgtag cgctgacggt agcgggtctc ggtgtcggtc aggccgtgga acttgtccgg
    14041 cagcgggcgc agcgacttgg tcagcagacg cacgtcctgc atgtcgacgt acaggtcacc
    14101 cttgccggag cgggccagca cgccgctgac gccgacgatg tcgcccaggt cccagtgctt
    14161 gatcgcctcg agggtctcgg ccgacagggt cttgcggttg acgtacagct ggatacgccc
    14221 gctggtgtcc tggatgacca tgaacgcgcc gcggttgagc atgatgcgcc cggccacgct
    14281 gaccttgatc tgctgcgctt ccagcgcttc cttgccggcc tcggcgtact gcttctgcag
    14341 gtcgcccgcc tggctgtcgc ggcggaagtc gttggggaag ggaatcgcct ggctctcgcg
    14401 cagggcggca agcttttcct tgcgctgggc aatcagcttg ttctcttcct catggagggc
    14461 gtgctcgttc agcggttgtt cgctcatggt cgtcgtttcc gtcactgatt cgttccctct
    14521 ccccgtcggg gagagggtta gggagagggg tggtgccggg gccgatgcca gccccatcac
    14581 cccagccctc tcccccgcag gggagaggga gtaaagatca aaggccctgc ttcaggctcg
    14641 cttcgatgaa ctcgtccaga tcgccgtcca gcaccgcatc gcagttgctc tcttcgacgc
    14701 cagtgcgcag atccttgatc cgcgactggt cgagtacgta ggagcggatc tggtgacccc
    14761 agccgatgtc cgacttggtg tcttccagcg cctgggcggc ggcgttgcgc ttctgcactt
    14821 ccagctcgta cagcttggcc cgcagcatct tcatggcggt gtcgcggttg gcgtgctggg
    14881 agcgctcgtt ctggcagctc accacggtat tggtggggat gtgggtgatc cgcaccgccg
    14941 agtcggtggt gttcacgtgc tggccaccgg caccggagga gcggtaggtg tcggtgcgca
    15001 gatcggccgg attgatgtcg atctcgatgt tgtcgtcgat ttccggcgag gcgaacaccg
    15061 cggtgaacga ggtgtggcga cggttgccgg agtcgaacgg actcttgcgc accagacggt
    15121 gcacgccgat ttcggtgcgc agccagccga aggcgtactc gcccttgata tgcacggtgg
    15181 cgcccttgat gccggcgact tcgccagcgg acagttccat gatggtggct tcgaaaccgt
    15241 gcttgtcggc ccagcgcagg tacatgcgca gcagcatgtt ggcccagtcc tgggcctcgg
    15301 tgccgccgga accggcctgg atgtcgaggt aggcgttgtt ggcgtccatc tcgccgctga
    15361 acatgcggcg gaactccagt ttctcgagga tctcgcgcag gcgctcgacc tcggtggcga
    15421 cgtcgttcac cgcgccttcg tcgttttcct cgacggccat ggccagcagg tcgctggagt
    15481 cggccaggcc gctggccagt tcgtcgaggg tctcgacgat ctgcgccagc tgggcgcgct
    15541 cacggcccag attctgcgcg tattccggat tgttccagac gttcgggtct tccagctcgc
    15601 gattgacttc ggtcagacgg tcatgcttct gatcgtagtc aaagataccc ccgaatagtc
    15661 tgggtgcggc tggtcaggtc cttgatgctg ttaacgatcg gattgatttc catggctggc
    15721 gtcactcgcg ggcggaacta gagaaaagcg caaaagtata cgccagtcac ggcgcgctgg
    15781 cagcacacgc gcgacaaagc acgctgggca gctgatgaca acaccctacg cccagccgta
    15841 gggtgcgccg cgcgccccac aacgccgcag gtgcgcacgg cgcaccctac ccaccgagct
    15901 cggccaggcg ggcgaggaag gctgcctcgt ccagcaccgc tacgcccagc tcgctggcct
    15961 tggtcagctt ggagccggcg ccagggccgg caaccacgca atgggtcttg gccgataccg
    16021 agccggcgac cttggcgccc agcgcctcca gccgtgcctt gccctcatcg cggctcatgg
    16081 cctcgagcgt gccggtgagc acccaggtct gccccgccag cggcaggccg gcgaccgcct
    16141 tcttctcgct ctgccagtgc atgccgaact cgcgcagctg cgcctcgatg gcaagggcat
    16201 gcgccacatt ggccggcgcg gcgaagtgct cgcgcagcgc ctgccgggcc ttctcgctga
    16261 gccccttggt cacgctcagg ctcagccagt cctggctgag ggcgatcacg ccctcgaggc
    16321 tgccggccgc gtcggcgagg ttcttcgccc cggtggcggc aatcccggcg atgttcagct
    16381 tgttgagcag ctcggccagc gtggcgcagg cagcgaactc ggcgtgcagc gcaccctcct
    16441 cctgcagcac cacgccgcgc gccagcagct gggcgatcac cgtgcggttg tgctcgtcct
    16501 cgaagaagct gtggatctcg tgggccactt ccaggccgac gtccggcagc caggtcagca
    16561 cctccggcag ggccaccgcg atgcgcgcca gcgcaccgag ggcgcgagcc agcagcttgg
    16621 ccgtctcctc gccgacatcg gggatgccga gggcatagat gaagcgcgcc agggtgggcc
    16681 gcttgctggc gtcgatggcc gccagcaggt tgcgggtgga cagttcggcg aagccctcca
    16741 gcaccaccac ctgctcgaag gtcagtgtgt agagatcggc cggcgagcgg accagcccca
    16801 cgtcgaccaa ctgttcaacg atcttgtcgc cgaggccgtc gatatccatg gcgcggcgcg
    16861 agacgaagtg gatgatcgcc tgcttgagct gcgcctggca gctcaggcgg ccgacgcagc
    16921 ggtagatcga gccctcgctg accgaggcct tgcccttgcc gcgcttgatc agctgggtac
    16981 gctcgacatg cgagccgcat accgggcagc tctgcggaat cgccaccggc cgcgcatcgg
    17041 ccggacggcg ctcgagcacc acctgcatga cctgcggaat cacgtcgccg gcgcggcgga
    17101 tgatcaccgt atcgccgatc atcagccc
//
LOCUS       DATCUN010000118         6633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_268016, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000118 DATCUN010000000
VERSION     DATCUN010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268016"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VIO83_04690"
     CDS             complement(<1..474)
                     /locus_tag="VIO83_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330608.1"
                     /translation="MREYDPYRPPQAPLLDQQLPAAFADGDWSCSQLRVLGALALGYL
                     FGLFVLLVLSLWQVLHPQFAPLAFTHLLSLLLVLLWGYLLLRLRALLAARFALHGLDW
                     PVALQILLGLLATGFAALHDGQAAGLAGWWELGYFALFVALGFSLLWFALRLRRIR"
     gene            complement(517..1215)
                     /gene="pyrF"
                     /locus_tag="VIO83_04695"
     CDS             complement(517..1215)
                     /gene="pyrF"
                     /locus_tag="VIO83_04695"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY8330609.1"
                     /translation="MTCQTPIIVALDFPAREAALALAERLDPQLCRVKVGKELFTRCG
                     PQIVETLAGKGFEVFLDLKFHDIPNTTAMAVKAAAELGVWMVNVHCSGGLRMMAACRE
                     TLDTLSGPKPLLIGVTVLTSMEREDLAGIGLDVEPQEQVLRLAGLAAQAGLDGLVCSA
                     QEAPALKAAQPQLQLVTPGIRPAGSAQDDQRRILTPRQALDAGSDYLVIGRPISQAAD
                     PAAALAAVVAELRG"
     gene            1350..1529
                     /locus_tag="VIO83_04700"
     CDS             1350..1529
                     /locus_tag="VIO83_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2897 family protein"
                     /protein_id="HEY8330610.1"
                     /translation="MPWYAWLFLALAFGSVVGSLLLLRDSANKMPLSEEELERMHKRN
                     AEQDAKARQDDGEDE"
     gene            complement(1549..1737)
                     /locus_tag="VIO83_04705"
     CDS             complement(1549..1737)
                     /locus_tag="VIO83_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330611.1"
                     /translation="MATGSEALPGEPQALLPPLFESKGESNSFELGGRLITNEPGQAD
                     EDSWHSVEGAELQLQFRR"
     gene            complement(2230..3042)
                     /locus_tag="VIO83_04710"
     CDS             complement(2230..3042)
                     /locus_tag="VIO83_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8330612.1"
                     /translation="MNPSVFRPLWLGVVVLLASCATAHPDAQQALAQARSSYQSLASD
                     PAVLAAAPKDVTRAGDSLARAERLADYWGSEEDVAHYAYLSQRYGAIAREHGMQAAEQ
                     QQLERLRLERERLQLALREAKLLGLQQDDSLAEQLVALAATETERGLVVSLGDVLFDS
                     GRAELKFSANRIVLKLYRFLELNPQRIVRIEGYTDSHGKAADNLQLSRARAQTVADVL
                     VDLGIDAKRIEVRGYGEAFPLAANASSYGRAQNRRVEIVFSDAQGKLGAERQ"
     gene            complement(3039..3389)
                     /locus_tag="VIO83_04715"
     CDS             complement(3039..3389)
                     /locus_tag="VIO83_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HEY8330613.1"
                     /translation="MTQRIFALLALLLLSGCASDPAPTAQLQLAEQAVTQARAVGTDP
                     EQEELRQAEAELAAARQAFARKDYRKARMLAERAELDARLVEAQTLTGKTRAQLTELK
                     SSIVRLRTQLGDLQ"
     gene            complement(3407..4222)
                     /locus_tag="VIO83_04720"
     CDS             complement(3407..4222)
                     /locus_tag="VIO83_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8330614.1"
                     /translation="MTIRSTLSRLGLALLVLAGSSHVMAAGKCERLIATGNPDQPPYL
                     WRDPQQPQRLIGASADLLMELGKALEVKIEVIDAGKRSAAEGDAVSGRVDLLAGSYLT
                     PARLGQFDFVHPAYLEVPVHVWVQRDKAPLFGRRADLASHSGLAIGAGSFSAALAQQV
                     DELKLKRTGKASDALWQLQQGKVDYLLLERNAGVALVERLGLGGELLALEPALGSEPL
                     YLALSHNSACNDPWLRGQLAKKMTEFRAAGLPQTLLQRNLERWKSQAQAPAIP"
     gene            complement(4370..5299)
                     /locus_tag="VIO83_04725"
     CDS             complement(4370..5299)
                     /locus_tag="VIO83_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY8330615.1"
                     /translation="MAILVIAEHNNAALGAATLNTVAAAQAIGGDIHVLVAGAGCAAV
                     AEAAAKVAGVSKVLVADSAALAHQLPENLAPLVAELGKGYSHVLAAATTTGKNVLPRV
                     AALLDVDQISEIIKVLAPDTFQRPIYAGNAIATVQSSAAVKVITVRGTGFDAAAAEGG
                     SASIEAIGAGADAGISSFVGEELAKSERPELTAAKVIVSGGRGMQNGDNFQLLYKLAD
                     KLGAAVGASRAAVDAGFVPNDMQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIV
                     AINKDEEAPIFQVADYGLVGDLFDLVPELEKAL"
     gene            complement(5299..6048)
                     /locus_tag="VIO83_04730"
     CDS             complement(5299..6048)
                     /locus_tag="VIO83_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY8330616.1"
                     /translation="MKILVAVKRVVDYNVKVRVKADNSGVELANVKMSMNPFCEIAVE
                     EAVRLKEKGIATEIVAVSVGPTQAQEQLRTALALGVDRAILVETAEELNSLAVAKLLK
                     AVVDKEQPQLVILGKQAIDSDNNQTGQMLAALTGFAQGTFASKVEVAGDKVNVTREID
                     GGLQTVALNLPAIVTTDLRLNEPRYASLPNIMKAKKKPLDVVTPDALGVSTASTVKTL
                     KVEAPAARSAGIKVKSVAELVEKLKNEAKVI"
     gene            6367..>6633
                     /locus_tag="VIO83_04735"
     CDS             6367..>6633
                     /locus_tag="VIO83_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8330617.1"
                     /translation="MEREYMEFDVVVVGAGPAGLSAACRLKQKAAEAGQELSVCVVEK
                     GSEVGAHILSGAVFEPRALNELFPDWKDLGAPLHTPVKRDDIYLF"
BASE COUNT         1092 a         2235 c         2264 g         1042 t
ORIGIN      
        1 gcggatgcgc cgcaggcgca gggcgaacca cagcaggctg aagccgaggg cgacgaacag
       61 ggcgaagtag ccgagctccc accaccccgc caacccggcc gcctggccgt cgtgcagcgc
      121 agcgaagccg gtcgccaaca agccgagcag gatctgcagc gccaccggcc agtccagccc
      181 gtgcagcgcg aagcgcgcgg ccagcaaggc acgcaagcgc agcagcaggt agccccagag
      241 caacaccagc agcaggctga gcagatgggt gaaggccagc ggcgcgaatt gcggatgcag
      301 cacctgccac aggctcagca ccagcagcac gaacagaccg aacagatagc ccaacgccag
      361 cgcgccgagc acgcgcaact ggctgcagct ccagtcgccg tcggcgaagg ccgccggcag
      421 ctgctggtca agcagcgggg cctgcggcgg acggtaggga tcgtactcgc gcatcgaccg
      481 tcccctcccc ggctgcgact gcgttatggg tcgacctcag ccgcgcagtt cggccaccac
      541 cgccgccagc gcggcggccg gatcggcagc ttggctgatc ggccggccga tcaccaggta
      601 gtcggagccg gcatccagcg cctggcgcgg ggtgagaatg cgccgctggt cgtcctgggc
      661 actgccggcc gggcggatac ccggggtcac cagctgcagc tgcggctgcg ccgccttcag
      721 cgccggcgcc tcctgggcgg agcagaccag cccgtcgagg ccggcctggg cggcgagacc
      781 cgccagacgc agcacctgct cctgcggctc gacatccagg ccgatgccgg ccagatcctc
      841 gcgctccatg ctggtcagca cggtcacgcc gatcagcagc ggcttgggcc cgctcagggt
      901 gtcgagcgtc tcgcggcagg ccgccatcat gcgcaggccg ccggagcagt gcacgttgac
      961 catccacacg ccgagctcgg cggcggcctt gaccgccatc gccgtggtgt tggggatgtc
     1021 gtggaatttc agatcgagga acacctcgaa gcccttgccg gccagcgtct cgacgatctg
     1081 cgggccgcag cgggtgaaca gctccttgcc gaccttgacc cggcacagtt gcggatcgag
     1141 gcgctcggcc agggccagtg cggcttcacg ggcggggaaa tccagggcga cgatgatcgg
     1201 ggtctggcag gtcatggggc tctctcggca agtcgaatcg ccgcgcattg taacgcaagc
     1261 ccgcgccggc cggcgcggcg ccagatcggg caagacagcc gcggcgcgcg gcgctatggt
     1321 gaagacgacg tcaaccgtag gagaaaatca tgccttggta tgcctggttg tttctggccc
     1381 tggccttcgg ctcggtggtc ggcagcctgc tgctgctgcg cgacagcgcc aacaagatgc
     1441 cgctcagcga ggaggagctg gaacgcatgc acaaacgcaa cgccgaacag gacgccaagg
     1501 ctcgccagga cgatggcgag gacgaatagt cccgcgcggt gccccggctc agcggcggaa
     1561 ctgcagctgc agctcggcgc cctccaccga atgccagctg tcctcgtccg cctgcccggg
     1621 ctcgttggtg atcaaccgac caccgagctc gaagctgttc gactcgccct tgctctcgaa
     1681 caggggcggc aacagggcct gtggctcgcc gggcagcgcc tcactcccgg tcgccaagcc
     1741 gaagccctcc ggcgtttcgc tgggcagact gaggtccagc ggcgccgcgg gttccgccag
     1801 ctttggccga gcagagacgg ccgcctctgc ggccggcggg cgggcacgct caagcggctt
     1861 ggagggcggc tcgggtagcg cgctgggcgc ggggggcact ggcggcggcg caggcggagc
     1921 ggtttctccc gcaggcggca gtggggcagg agctggagct ggagctggag ctggaggcgt
     1981 atcgggcgcc ggtgccgtgg gctgcggcag ggtggccgca ggtggctccg gctgctgcgg
     2041 ttggtctttc tcgcgatcgg cgtcgcaggc gaccaggccg agcgacagca gcaagaccag
     2101 acatgagcgc acgactgtca tcacggacct cgcagtaacg gcggccgggg cgaaccggcc
     2161 gccagctttc ggtgacgact gcccgcgcca cgccgaacac acccggcatc accgcggcac
     2221 ggacacaacc tactggcgct cggcgccgag cttgccctgc gcgtcggaga acacgatctc
     2281 gacccggcga ttctgcgccc gtccatagga tgaggcattg gccgccagcg ggaaagcctc
     2341 gccgtagccg cgcacctcga tgcgcttggc gtcgatcccc agatcgacca gcacgtcagc
     2401 gaccgtctgc gcccgcgcac gggacagctg cagattgtcg gcggccttgc catggctgtc
     2461 ggtatagccc tcgatgcgca cgatgcgctg cgggttgagc tccaggaagc ggtacagctt
     2521 gagcacgatg cgattggcgc tgaacttcag ctcggcgcgg ccactgtcga acagcacatc
     2581 gcccagactc accaccagac cgcgctcggt ctcggtggcg gccagcgcga ccagctgctc
     2641 cgcgagcgag tcgtcctgtt gcagaccgag cagcttggcc tcgcgcaggg ccagctgcag
     2701 acgttcgcgc tccagccgca ggcgctccag ctgctgctgt tccgccgcct gcatgccgtg
     2761 ctcacgggca atagccccgt aacgctggct caggtaggcg tagtgcgcaa catcttcctc
     2821 gctgccccag tagtcggcca ggcgctccgc acgcgccagg gagtcgccgg cgcgagtcac
     2881 atccttgggc gcggcggcca gcacggcagg gtcgctggcg agcgactggt agctgctgcg
     2941 ggcctgcgcc agggcctgtt gcgcgtccgg atgggcggtc gcgcagctgg ccagcaggac
     3001 aacgacgccc agccacagcg ggcgaaagac ggatgggttc actgcaggtc tcccagctgg
     3061 gtgcgcaggc gcacgatgct gctcttcagc tcggtgagct gcgcgcgggt cttgccggtc
     3121 agggtctgcg cctcaaccag tcgggcgtcc agctcggcac gttcggcgag catgcgcgcc
     3181 ttgcggtagt ccttgcgcgc gaaagcctgg cgcgccgcag ccagctcggc ctcggcctgg
     3241 cgcagctcct cctgctcggg gtcggtaccg accgcgcgcg cctgcgtcac ggcctgctcg
     3301 gccagttgca gctgcgcggt cggcgccgga tcgctggcgc agccgctcag gagcagcagc
     3361 gccagcaggg caaaaatgcg ttgggtcatc gaatgaacat cctgcttcag ggaatcgccg
     3421 gcgcctgcgc ctggctcttc cagcgctcaa gattgcgctg tagcagggtc tgcggcaggc
     3481 cggcggcgcg gaattctgtc atctttttcg ccagctgtcc gcgcagccag ggatcattgc
     3541 aggccgagtt gtgcgacagg gccaggtaca gcggctcgct gcccagcgcc ggctcaaggg
     3601 ccagcagctc gccccccagc cccagacgct cgaccagcgc cacgccggca ttgcgctcga
     3661 gcagcaggta gtcgaccttg ccctgctgca gttgccacaa ggcatcgctg gccttgccgg
     3721 tgcgcttgag cttgagctcg tcgacctgct gcgccaacgc ggcggagaaa ctgccggcac
     3781 cgatcgccag accgctgtga ctggccagat cggcccgccg gccgaacagc ggcgccttgt
     3841 cgcgctgcac ccagacgtgt accggcacct cgagataggc cggatggacg aagtcgaact
     3901 ggccgagacg cgccggcgtc aggtagctgc ccgccaacag atcgacccgc ccgctgacgg
     3961 cgtcgccctc ggcggcggag cgcttgccgg catcgatcac ctcgatcttc acctcgaggg
     4021 ccttgcccag ctccatgagc aggtcggcgc tggcgccaat cagccgctgc ggttgctgcg
     4081 gatcgcgcca cagatagggc ggctggtccg gattgccggt ggcgatcagg cgctcgcact
     4141 tgccggcggc catgacatgg ctggagccgg ccagaaccag cagcgccagt cccaggcgcg
     4201 acagggtgga acggatggtc atgacgaacc tcgcaagtcg gttgccggcg ccgccggaaa
     4261 agatggcggc tagtttaggg cagagcggcc cgccgccggc aagcggattt cccccgagca
     4321 caaaaaaccc gctccggaga gcgggttttt cggcacagcc cgaaagacct tacagggcct
     4381 tttccagctc cggcacgagg tcgaacaggt cgccgaccag gccgtagtcg gccacctgga
     4441 agatcggtgc ctcttcgtcc ttgttgatcg ccacgatcac cttggagtcc ttcatgcccg
     4501 ccaggtgctg gatggcaccg gagatgccca cggcgatgta cagctgcggg gcgacgatct
     4561 tgccggtctg gccgacctgc atgtcgttgg gcacgaagcc ggcatcgacg gcggcacgcg
     4621 aggcgccgac ggcggcgccg agcttgtcgg ccagcttgta gagcagctgg aagttgtcgc
     4681 cgttctgcat gccgcgacca ccggagacga tgaccttggc tgcggtcagc tccggacgct
     4741 cggacttggc cagctcctcg ccgacgaagc tggaaatgcc ggcatcggcg cctgcaccga
     4801 tggcctcgat gctcgcggaa ccaccttcgg cagctgcggc gtcgaagccg gtgccacgca
     4861 cggtgatcac tttcacggct gccgaggact gcacggtggc gatggcgttg ccggcataga
     4921 tcggccgctg gaaggtgtcc ggggcgagga ccttgatgat ctcggagatc tggtcgacat
     4981 ccagcagcgc ggcgacgcgc ggcagcacgt tcttgccggt ggtggtggcc gcagccagca
     5041 catggctgta gcccttgccc agctcggcga ccagcggggc gaggttttcc ggcagctggt
     5101 gagccagcgc ggcgctgtcg gcaaccagca ccttgctgac accggcgacc ttggcggccg
     5161 cttcggcgac ggcagcgcaa ccggcgccgg cgaccagcac atggatgtcg ccaccgatgg
     5221 cctgggcggc ggccacggtg ttcagggtcg cagcacccag cgctgcgttg ttgtgctcag
     5281 cgataaccag gatagccatc agatcacctt cgcttcgttc ttcagtttct cgaccagctc
     5341 ggccacggac ttgaccttga tgccggcact gcgggcagcc ggggcttcga ccttgagggt
     5401 cttcacggtc gaggccgtgg acacgcccag cgcgtcggga gtgaccacgt cgagcggctt
     5461 cttcttggcc ttcatgatgt tcggcagcga agcgtagcgc ggctcgttga ggcgcaggtc
     5521 ggtggtgacg atggccggca ggttgagggc cacggtctgc aggccgccgt cgatttcgcg
     5581 ggtgacgttg accttgtcgc cggcgacctc gaccttggag gcgaaggtgc cctgggcgaa
     5641 gccggtcagc gcggcgagca tctggccggt ctggttgttg tcgctgtcga tggcctgctt
     5701 gccgaggatg accagctgcg gctgctcttt atcgacgacg gctttcagca gcttggccac
     5761 ggccagggag ttcagctcct cggcggtttc caccaggatg gcgcgatcga caccgagggc
     5821 cagggcggta cgcagctgct cctgggcctg ggtcgggccg acggacacgg cgacgatctc
     5881 ggtggcgatg cccttctctt tcagacgcac cgcttcttcc acggcgatct cacagaacgg
     5941 gttcatcgac atcttgacgt tggccagctc gacgccggag ttgtccgcct tgacgcgaac
     6001 cttgacgttg tagtcgacca ctcgtttgac agctaccaga atcttcatgg attcctcgtg
     6061 ttttccggtg agttgatatg gtatcgccag ggcgtaccag gccgggctgc ccgtcgggtg
     6121 gaaccgactc tcagctatac cgcgaccagc aaaaacatcg tatgttgacc gcatccccta
     6181 ccccggtcaa tacgacaaaa cttccactgt gtaagcgcca gactttaaga taagcgcatc
     6241 agccggcgct cgaacaaacg cttgtaccgg tcttgccata catcgtaaat atattgcgct
     6301 gcacaaaaac cgcccttgcg gactccaaga agcagattgc cagccttgag ttaggagaat
     6361 tagagagtgg aacgcgaata catggaattc gacgtcgtcg tcgtcggtgc cggccctgcc
     6421 ggactttccg ccgcctgccg cctgaagcag aaggccgccg aagccggcca ggagctcagc
     6481 gtctgcgtgg tcgagaaagg ctccgaagtc ggcgcccaca tcctctccgg cgcggtgttc
     6541 gagccgcgcg cgctcaacga gctgttcccc gactggaagg acctcggtgc gccgctgcac
     6601 accccggtca agcgcgacga catctacctg ttc
//
LOCUS       DATCUN010000119        16409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_43179, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000119 DATCUN010000000
VERSION     DATCUN010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 16409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16409
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43179"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..596
                     /locus_tag="VIO83_04740"
     CDS             195..596
                     /locus_tag="VIO83_04740"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY8330618.1"
                     /translation="MNDFEQDDPIPQGDLALQITALPRETNGFGDIYGGWLVAQMDLA
                     GTAMASRIAGGRVATVAIDRMAFLVPVAVGAQLSFYTQAVACGRSSIRLLIEVWSDDP
                     LSSEWRKVTEAVFVFVAIDGSGRTRPMPPRS"
     gene            complement(678..1784)
                     /gene="aguA"
                     /locus_tag="VIO83_04745"
     CDS             complement(678..1784)
                     /gene="aguA"
                     /locus_tag="VIO83_04745"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEY8330619.1"
                     /translation="MTTLNTPPRADGFRMPAEWEAHAQTWLLWPERPDNWRLGAKPAQ
                     AAFAAVAKAIARFEPVTVGVSASQYENARARLSHGDIRVLELSSDDAWVRDSGPTFVV
                     NEGGEVRGVDWTFNAWGGFEGGLYAPWHRDDQVAGKILAIERCARYRTDGFVLEGGAI
                     HVDGEGTLLTTEECLLNPNRNPQLSRGEIEAVLHAHLAVDKVIWLPHGLFNDETDGHV
                     DNFCCFVRPGEVLLAWSDDERDPNWSRCRAALAVLEQARDARGRALRVHKMPIPGPLY
                     ATAEECAGVDWVAGSQPRDPSIRLAGSYVNFLIVNGGIIAPSFADPHDAGAEAILQRL
                     FPEREVVMVPGREILLGGGNIHCITQQQPAAQRR"
     gene            complement(1871..2752)
                     /gene="aguB"
                     /locus_tag="VIO83_04750"
     CDS             complement(1871..2752)
                     /gene="aguB"
                     /locus_tag="VIO83_04750"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEY8330620.1"
                     /translation="MSRIVTVAATQMACSWERAANIANAEKLVRQAAARGAQIILLQE
                     LFETPYFCQKPNPDYLQLATPVETNEAIAHFRKVARELKVVLPISFFERAGRARFNSV
                     AIIDADGSLLGVYRKSHIPDGPGYHEKYYFNPGDTGFKVWNTCYARIGVGICWDQWFP
                     ECARAMALQGAELLFYPTAIGSEPHDPNISSRDHWQRVQQGHAGANLMPLIASNRIGR
                     EDQDDYHITFYGSSFIADPFGAKLAELGEHEEGILLHSFDLDHLEHVRSAWGVFRDRR
                     PNLYGALGTLDGTLKSE"
     gene            complement(2985..4361)
                     /locus_tag="VIO83_04755"
     CDS             complement(2985..4361)
                     /locus_tag="VIO83_04755"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY8330621.1"
                     /translation="MLPAPSAVLLNEANEFLAEHPEVQFVDLLIADMNGVVRGKRIDR
                     SMLVKVYEKGINLPASLFALDIKGSTVESSGLGLDIGDADRVCFPIPGTLCMEPWQRR
                     PTAQLLMTMHEMAGKPFFADPREVLRQVVQRFTDMGLTVCAAFELEFYLIDQENVNGR
                     PQPPRSPISGKRPQAVQVYSIDDLDEYADCLRDILDGAHIQGIPADAIVKESAPAQFE
                     VNLNHVADPMAACDYAVLLKRLIKNTAYDHEMDTTFMAKPYPGQAGNGLHVHISLLDP
                     DGNNIFAGEDALQNESLRHAIGGVLQTLPASMAFICPNVNSYRRFGSQFYVPNAPSWG
                     LDNRTVALRVPTGNPDALRIEQRVAGADANPYLLMASLLAGVHHGLTHLIEPGEPIEG
                     NSYEQLEQSLPNNLRDALRALDDSEILARYINPDYIDIFVACKEHELEEFEHSISDLE
                     YNWYLHTV"
     gene            4630..5382
                     /locus_tag="VIO83_04760"
     CDS             4630..5382
                     /locus_tag="VIO83_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY8330622.1"
                     /translation="MSRQPLIGVSACSKQIGPHPFHIAGDKYLRAIAIGAGGLPLVIP
                     ATPELIDIPDLLERLDGLLLTGSPSNVEPQHYQGPDSAPGTHHDPARDNLTLPLIRAA
                     VDAGVPLLGICRGFQEMNVAFGGSLHQRVHEVGPFMDHRDDPEQPLEVQYGLRHPLLV
                     QPGGLLDQLGLAPQFEVNSVHGQGVERLAPGLRVEGVAPDGLVEAFSVERARTFALGV
                     QFHPEWQVTEHPAYLAIFRAFGDACRERAARH"
     gene            5420..6778
                     /locus_tag="VIO83_04765"
     CDS             5420..6778
                     /locus_tag="VIO83_04765"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY8330623.1"
                     /translation="MASKQDRLSSWLQERRIIEVECLVSDLNGIARGKIAPTSKFLAE
                     RGMRLPESVLLQSVTGDYVEDEVYYELLDPADIDMFCRPDDNAMYPVPWAAEPTALVI
                     HDSYDKVGNPTDLSPRNVLKHVLALYAEQGWQPIVAPEMEFYLTQRCDDPDYPLQPPV
                     GRSGRQETGRQSFSIDAANEFDPLFEDMYAWCEAQGLDLDTLIHEEGTAQMEINFRHG
                     EALHLADQIFVFKRTLREAAFKHGVTATFMAKPMTGEPGSAMHLHQSVVDRASGHNVF
                     SSADGSMSELFLNHIAGLQKLIPEALPLFAPNVNSFRRFLPGTSAPVNVEWGEENRTA
                     GLRVPDANPQNRRVENRLPGADANPYLAIAASLLCGYVGMVEGLHPSPPVKGRAYQRR
                     NLRLPLNIEAALEAMEQSKTLERYLGRRFCRGYVAVKRVENENFKRVISSWEREFLLL
                     SV"
     gene            6820..8181
                     /locus_tag="VIO83_04770"
     CDS             6820..8181
                     /locus_tag="VIO83_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY8330624.1"
                     /translation="MTQQRTTAQWQALGREHLLAPFSDYKQLGAKGARIVTRADGIYL
                     WDSEGQQILDAMAGLWCVNVGYGRRELADAAHRQMLELPYYNLFFQTAHPPAIELAAA
                     IAELAPEGMNHVFFTGSGSEANDTVLRLVRHYWAIKGQPRKKVVIGRVNAYHGSTVAG
                     ASLGGMAAMHAQGDLPIPGIEHIAQPYWYGEGGAMDPQAFGEWAANELEKKILEVGED
                     RVAAFIAEPIQGAGGVIIPPDSYWPKVRAILAKYDILFVVDEVICGFGRTGEWFGSQY
                     YGLEPDLMSIAKGLTSGYLPMGGVIVRDTVAQTLAEGGEFYHGYTYSGHPVAAAVALE
                     NIRILREEKIVERVKRETAPYLQRRWQELADHPLVGEARGLGMLGALELVKDKQTRAR
                     FEGQGVGMRCREHCFANGLIMRAVGDTMIISPPLVITPTQIDDMLLLVRKCLDATAAE
                     VLG"
     gene            8356..9453
                     /locus_tag="VIO83_04775"
     CDS             8356..9453
                     /locus_tag="VIO83_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8330625.1"
                     /translation="MKAFAKTLLAATLAATVAGAAQADDKVLHVYNWSDYIAPQTVEK
                     FEKQTGIKVVYDVYDSNETLEAKLLAGKSGYDIVVPSNNFLAKQIKAGVYQKLDKAQL
                     SNWQNLNKDLLKTLEVSDPGNQYAAPYMWGSIGIGFNPDKVKAALGADAPVNSWDLLF
                     KPENIEKLKGCGVSFLDSPTEMLPIALHYLGYKSDSQDPKELKAAEELFLKIRPSVTY
                     FHSSKYISDLANGNICVAVGYSGDINQAKARAEEAKGGIKVSYNIPQEGAGTFFDMLA
                     IPADAGNPAGAHAFMNFLMQPEVMAEITNFVQFPNANAAATALVDEAIRTDPGVYPSD
                     EVMKKLYTTTDLPAKVQRTITRSWTKIKSGK"
     gene            9699..10850
                     /gene="potA"
                     /locus_tag="VIO83_04780"
                     /pseudo
     CDS             9699..10850
                     /gene="potA"
                     /locus_tag="VIO83_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248993.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
     gene            10847..11749
                     /locus_tag="VIO83_04785"
     CDS             10847..11749
                     /locus_tag="VIO83_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8330626.1"
                     /translation="MNSLRQLLPRGRHLVIGVPFFWLLLFFLLPFAIVLKISFAEVDV
                     AIPPYTALLQYLDQQLQVVLNLGNYLFLAEDELYLAAYLGSLKIALVGTLLCLLIGYP
                     MAYAIARASKAMQTVLLLLIMMPTWTAILIRVYAWMGILGNNGLLNNLLLGLGLIDTP
                     LLILNTDLAVYIGVVYAYLPFMVLPLYANLVKHDPSLLEAAADLGARHLTSFWKITVP
                     LSKNGIIAGCMLVFIPVVGEFVIPELLGGPETLMIGKVLWQEFFNNRDWPVASALAVV
                     MLAILMVPIILFNRNQAKELEGRV"
     gene            11832..12680
                     /locus_tag="VIO83_04790"
     CDS             11832..12680
                     /locus_tag="VIO83_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8330627.1"
                     /translation="MNKRFSFSGCMLVAGLLFIYVPMLILVLYSFNSSRLVTVWGGWS
                     LKWYAGLLDNSQLMGAVLRSLEIALYTAIAATALGTLAAFVLTRIPRFRGRTLFGGLV
                     TAPLVMPDVITGLSLLLLFVAMAQLIGWPAERGLLTIWIAHTTFCSSYVAVVVSARLR
                     ELDLSIEEAAMDLGARPWKVFFLITVPMIAPSLAAGGMMSFALSLDDLVLASFVSGPG
                     ATTLPMEVFSAVRLGVKPEINAVASLILLVVSLFTFLAWFFSHRAEERRKRAILQAME
                     EMVKEH"
     gene            complement(12843..13475)
                     /locus_tag="VIO83_04795"
     CDS             complement(12843..13475)
                     /locus_tag="VIO83_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY8330628.1"
                     /translation="MTRIHKTVLILVAAIALVLGLTVSKVLNAPSQSDPAALADAGIV
                     LLPQSRALPALTLTDENGQPQAVDQLKGRWTLLFFGYTFCPDICPTTLAELRQLKSQL
                     EPEALARLQVTMVSIDPHRDTPAQLKQYLGYYKAGYSGLTGELADIQALANAVSIPFI
                     PGDTSREHYTVDHSGNLAVIGPDGRQRGFIRAPLNVAKLAVQLPALLKQD"
     gene            complement(13472..14386)
                     /gene="cyoE"
                     /locus_tag="VIO83_04800"
     CDS             complement(13472..14386)
                     /gene="cyoE"
                     /locus_tag="VIO83_04800"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY8330629.1"
                     /translation="MTTLVHEHPAHAGWRDYLELTKPKVVVLMLITALVGMFLATDSG
                     VPWRVLLFGNLGIGLCAGAAAAVNHVVDRRIDAIMARTRLRPLVAGRVSPRAALAFAL
                     LLALAGQALLLAFTNALTAWLTLASLLGYAALYTGFLKRATPQNIVIGGLAGAAPPLL
                     GWTAVTGQLDAEALLLVLIIFAWTPPHFWALAIHRKDDYAKAEIPMLPVTHGEHYTKL
                     HILLYTLILFAVSLLPFVIHMSGPLYLAGAVLLGARFLDWAWALYRDSRPQAAIKTFK
                     YSITYLFALFIVLLADHYLPPSLLNLSP"
     gene            complement(14419..15471)
                     /locus_tag="VIO83_04805"
     CDS             complement(14419..15471)
                     /locus_tag="VIO83_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004575260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY8330630.1"
                     /translation="MAPTTRPGYRLALCATALALLVVLLGAYTRLTHAGLGCPDWPGC
                     YGFLGVPQSEAQLAHAERHFPEAPVEAQKGWNEMVHRYFAGSLGLLIAVLAVQAIRRG
                     GRDGQPLKLPLALFALVLLQAAFGMWTVTLKLWPQVVSAHLLGGFATLSLLFLLSLRL
                     SGRLPPLAGVLQPLRALAALALLVVIGQIALGGWVSSNYAAVACVDLPTCHGEWWPAT
                     DFANGFHLTQHIGPNYLGGQLDSEARTAIHLSHRLGAVLVSLLLLVLAGQLLRHGRPR
                     LAGLLLVALLAQVSLGVANVLLHLPLAVALAHNLGGATLLLVLVLINYRLRVPVALKM
                     DNGLRLVARGSLRRTG"
     gene            complement(15594..16181)
                     /locus_tag="VIO83_04810"
     CDS             complement(15594..16181)
                     /locus_tag="VIO83_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330631.1"
                     /translation="MGSTLTLEQVARRPGRGRWQLLGLLAVVIGPMLLASAMYRWQFW
                     VPQTRSYHGELIATGQTPADLGVAGAWGSAVDDDQPRWQLLVTSADDCRADCRELVYL
                     ARQIHIGLNREVSRAGHVLAHGAALPADYAAELHTAYPQLRLLELDGERYPEAGAQLW
                     IVDPHGNLVLRYPAGSNGKQILEDLRYLLKISQIG"
     gene            complement(16159..>16409)
                     /locus_tag="VIO83_04815"
     CDS             complement(16159..>16409)
                     /locus_tag="VIO83_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014850992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HEY8330632.1"
                     /translation="VNAVDAAGLWRELGRAGLADELRLQPGPASYLGDWPLVSMGPEK
                     HLGYAVQWFALAATLLGLFVWFGVQQAREVRDGLHSDT"
BASE COUNT         2762 a         5556 c         5513 g         2578 t
ORIGIN      
        1 atggtcttga tcgggggcca gtatcggcag gccgtatgaa cactctatga atcgattgtg
       61 acaattgcgt gaccgggcat ttcgccaccg gcaaccgtcc ggcggtcgtt tacatccgcc
      121 actgccggta tactctgcac aggcataatt gctgcactgc aacaacaata atccgcgccc
      181 gagcacccgc ccagatgaac gacttcgaac aggacgaccc cattccgcaa ggcgatctcg
      241 ccctgcagat cactgcgctg ccgcgcgaaa ccaacggctt cggcgacatc tatggcggct
      301 ggctggtcgc ccagatggac ctcgccggta ccgccatggc cagccgcatc gctggcggcc
      361 gggtggccac cgtggccatc gaccgcatgg ccttcctggt gccggtggcc gtcggcgcac
      421 agctgtcctt ctatacccag gccgtggcct gcgggcgcag ctcgatccgc ctgctgatcg
      481 aggtgtggag cgacgacccg ctgtccagcg aatggcgcaa ggtcaccgag gcggtgttcg
      541 tcttcgtcgc catcgacggc agcggccgca cccgccccat gccgccacgc agctgacaca
      601 ctaacgtcat tcaactgtag tcaaacatgg cgccgcgtcc agcggcagca cgccagccgt
      661 cgggctgcga cctgagctca gcgccgctgc gccgccggct gctgctgggt gatgcagtgg
      721 atgttgccgc cgccgaggag aatctcgcgg cccggcacca tcaccacctc gcgctcggga
      781 aacagccgct gcaggatggc ctcggcgccg gcatcgtgcg ggtcggcgaa gctcggcgcg
      841 atgatgccgc cgttgacgat caggaagttg acgtaggagc cggccaggcg gatcgacgga
      901 tcgcgcggct ggctgccggc gacccaatcc accccggcac actcctcggc ggtggcgtac
      961 aaggggccgg ggatcggcat cttgtgtacg cgcagcgccc gcccgcgggc atcgcgcgcc
     1021 tgctcgagca ccgccagcgc ggcgcggcag cgcgaccagt tgggatcgcg ctcgtcgtcg
     1081 ctccaggcca gcagcacttc gccgggacgc acgaagcagc agaagttgtc gacatggccg
     1141 tcggtctcgt cgttgaacag gccgtgcggc agccagatca ccttgtcgac cgccaggtgg
     1201 gcatgcagca ccgcctcgat ctcgccgcgg ctcagctgcg gattgcggtt ggggttgagc
     1261 aggcactctt cggtggtcag cagggtgccc tcgccgtcga catggatggc gccgccttcc
     1321 agcacgaagc cgtcggtgcg gtagcgcgcg cagcgctcga tcgcgaggat cttgcccgcc
     1381 acctggtcat cgcggtgcca cggcgcgtac agcccgccct cgaagccgcc ccaggcgttg
     1441 aaggtccagt ccacgccgcg cacctcgccg ccctcgttga ccacgaaggt cggcccgctg
     1501 tcgcgcaccc aggcatcgtc gctgctcagc tccagcacgc ggatgtcgcc gtggctcagg
     1561 cgggcgcggg cgttctcgta ctgactggcg ctgacgccga cggtcaccgg ctcgaagcgg
     1621 gcgatggcct tggccaccgc ggcgaaggcg gcctgtgccg gcttggcgcc gaggcgccag
     1681 ttgtccggcc gctccggcca gagcagccag gtctgcgcgt gcgcctccca ttccgccggc
     1741 atgcgaaaac cgtcggcgcg cgggggggtg ttgagggtgg tcatggcgaa tacctgtcga
     1801 gcttgatcgt tgccacgctc ccgaggggca acgcaatcgg aggcgctccg cgcccccacg
     1861 gatttgccca ttactcggac ttgagcgtgc catccagggt gccaagcgcg ccgtacaggt
     1921 tcggccgccg gtcgcggaac accccccagg cgctgcgcac gtgctcgagg tggtcgaggt
     1981 cgaagctgtg cagcaggatg ccctcctcgt gctcgcccag ctcggcgagc ttggcgccga
     2041 acgggtcggc aatgaacgag gagccataga aggtgatgtg atagtcgtcc tggtcctcgc
     2101 gaccgatgcg gttgctggcg atcagcggca tcaggttggc cccggcgtgg ccctgctgca
     2161 cgcgctgcca gtggtcgcgg gaactgatgt tggggtcgtg cggctcgctg ccgatggcgg
     2221 tcgggtagaa cagcagctcg gcgccctgca gggccatggc ccgcgcgcat tcggggaacc
     2281 actggtccca gcagatgccc acgccgatcc tggcgtagca ggtgttccac accttgaagc
     2341 cggtgtcgcc gggattgaag tagtacttct cgtgatagcc cgggccgtcg gggatgtggc
     2401 tcttgcggta gacgccgagc aggctgccgt cggcgtcgat gatcgccacg ctgttgaagc
     2461 gcgcgcggcc ggcgcgctcg aagaagctga tcggcagcac caccttcagt tcgcgggcga
     2521 ccttgcggaa atgggcgatg gcctcgttgg tttccacggg cgtggccagc tgcaggtagt
     2581 ccgggttggg cttctggcag aagtacgggg tctcgaacag ctcctgcagc aggatgatct
     2641 gcgccccgcg ggccgccgcc tggcgcacca gcttctcggc attggcgatg ttggcggcgc
     2701 gctcccagga gcaggccatc tgggtagcgg cgacggtgac gatgcgggac atgacgacct
     2761 cgggcgacgg ggaaacggct gccttcaagc tggcagagga tggcgccggg gtcaaatggc
     2821 cggcagaaac gggcgcgcca cagtccgctg gtcattcccc gtcagccgca gggtggatgg
     2881 ccaccccgtg gaaaaccacc gcaggtcttt tccaccctgc gggtcgcggg cctgtggtgg
     2941 aacatcgctg cgcgagtttt ccaccctggc ggctggccga tggttcaaac cgtatgcaag
     3001 taccagttgt actcgaggtc ggagatcgag tgctcgaact cctccagctc gtgctccttg
     3061 caggcgacga agatgtcgat gtagtcgggg ttgatgtagc gggcgaggat ctcgctgtcg
     3121 tccagcgcgc gcagggcgtc gcgcaggttg ttgggcaggc tctgttcgag ctgctcgtag
     3181 gagttgccct cgatcggctc gcccggctcg atcaggtggg tcaggccgtg gtgcacgccg
     3241 gccagcaagg aggccatcag caggtagggg ttggcgtcgg cgccggccac gcgctgctcg
     3301 atgcgcaggg cgtcgggatt gccggtgggc acgcgcaggg ccacggtgcg gttgtccagg
     3361 ccccaggacg gcgcgttggg cacgtagaac tgcgaaccga agcggcggta cgagttgacg
     3421 ttggggcaga tgaaggccat cgacgccggc agggtctgca gcaccccgcc gatggcgtgg
     3481 cgcagggatt cgttctgcag cgcgtcctcg ccggcgaaga tgttgttgcc gtccgggtcg
     3541 agcagcgaga tgtgcacgtg cagaccgttg ccggcctggc ccggataggg cttggccatg
     3601 aaggtggtgt ccatctcgtg gtcgtaggcg gtgttcttga tcaggcgctt gagcagcacg
     3661 gcgtagtcgc aggccgccat cgggtcggcg acgtggttga ggttgacctc gaactgcgcc
     3721 ggcgcacttt ccttgacgat ggcgtcggcg gggatgccct ggatgtgggc gccgtcgagg
     3781 atgtcgcgca ggcagtcggc gtattcgtcg aggtcgtcga tcgagtagac ctgcaccgcc
     3841 tgcgggcgct tgccggagat cggcgagcgc ggcggctgcg ggcggccgtt cacgttctcc
     3901 tggtcgatca ggtagaactc cagctcgaag gcggcgcaca cggtcaggcc catgtcggtg
     3961 aagcgctgca ccacctggcg cagcacttcg cgcgggtcgg cgaagaacgg cttgccggcc
     4021 atctcgtgca tggtcatcag cagctgcgca gtcggccggc gctgccaggg ctccatgcac
     4081 agggtgccgg gaatcgggaa acacacccga tcggcgtcgc cgatgtcgag gcccaggccc
     4141 gagctttcca cggtcgaacc cttgatatcc agggcgaaca gcgaggccgg taggttgatg
     4201 cccttttcat agaccttgac cagcatgctg cggtcgatgc gcttgccgcg caccacgccg
     4261 ttcatatcgg caatcagcag gtccacgaac tggacctcgg ggtgttccgc aaggaactcg
     4321 ttcgcctcgt tgagcagaac ggcactcggc gcgggcaaca tgatgggaca cctttcttgt
     4381 taaaaatttc aatcacatag aggcacggtt ttgagtcaat cccaaaggct gcgggttgtc
     4441 aataggccca gatgttgcac agtgtttggg catgacgagc catttcaggg cagtgcaagc
     4501 tcggcgaacg tgtggcgaac agggggcgtt catcacttcc atttgggcaa aacggcaaca
     4561 gttgtcgaat aaattgaaca aggctaatct cgggtcagac cccatcatta ccatgtcccc
     4621 gggtgcccca tgtcccgcca gcccctgatc ggcgtcagcg cctgcagcaa acagatcggc
     4681 ccgcatccct tccacatcgc cggcgacaag tacctgcgcg ccatagccat tggcgccggc
     4741 ggcctgccgc tggtcatccc ggccacgccc gagctgatcg acattccgga cctgctcgag
     4801 cgcctcgacg gcctgctgct caccggctcg ccgtccaacg tcgagccgca gcactaccag
     4861 gggccggaca gcgcgccggg cacccaccac gatccggccc gcgacaacct gaccctgccg
     4921 ctgatccgtg ccgcggtgga cgccggcgtg ccgctgctcg gcatctgccg cggcttccag
     4981 gaaatgaacg tggccttcgg cggcagcctg caccagcgcg tccacgaggt cggcccgttc
     5041 atggatcacc gcgacgatcc ggagcaaccg ctggaggtgc agtacggcct gcgccatccg
     5101 ctgctggtgc agccgggcgg cctgctcgac cagctcggcc tggcgccgca gttcgaggtc
     5161 aactcggtgc acggccaggg cgtcgaacgc ctggcgcccg gcctgcgcgt cgagggcgtg
     5221 gcgccggacg gcctggtcga ggcgttctcc gtcgagcgcg cgcgcacctt cgccctcggc
     5281 gtacagttcc acccggagtg gcaggtgacc gagcacccgg cctacctggc gatcttccgc
     5341 gccttcggcg acgcctgccg cgagcgtgcc gcccgacact gagccatgct gtaagggact
     5401 accgacgtgc gaggttgtta tggctagcaa gcaggaccgg ctgagcagct ggctgcagga
     5461 acgcaggatc atcgaggtgg agtgcctggt cagcgacctc aacggcatcg cccgcggcaa
     5521 gatcgcgccg accagcaagt tcctcgccga acgcggcatg cgcctgccgg agagcgtgct
     5581 gctgcagagc gtcaccggcg actacgtcga ggacgaggtc tattacgagc tgctcgatcc
     5641 cgccgacatc gacatgttct gccgccccga cgacaacgcc atgtacccgg tgccctgggc
     5701 cgccgaaccg accgcgctgg tgatccacga cagctacgac aaggtcggca accccaccga
     5761 cctgtcgccg cgcaacgtgc tcaagcacgt gctggcgctg tacgccgagc agggctggca
     5821 gccgatcgtc gcgccggaga tggagttcta cctgacccag cgctgcgacg accccgacta
     5881 cccgctgcag ccgccggtgg gccgctccgg gcgccaggag accggccgcc aatcgttctc
     5941 catcgacgcc gccaacgagt tcgacccgct gttcgaggac atgtacgcct ggtgcgaggc
     6001 gcagggcctg gatctcgaca ccctgatcca cgaggaaggc accgcgcaga tggagatcaa
     6061 cttccgccac ggcgaggccc tgcatctggc cgaccagatc ttcgtgttca agcgcaccct
     6121 gcgcgaggcg gcgttcaagc acggcgtcac cgccaccttc atggccaagc cgatgaccgg
     6181 cgagccgggt agcgccatgc acctgcacca gagcgtggtc gaccgggcga gcggccacaa
     6241 cgtgttctcc agcgcggacg gcagcatgag cgagctgttc ctcaaccaca tcgccggcct
     6301 gcagaagctg atccccgagg cgctgccgct gttcgccccc aacgtcaact cgttccgccg
     6361 cttcctgccc ggcacctcgg cgccggtcaa cgtcgagtgg ggcgaggaga accgcaccgc
     6421 cggcctgcgc gtgccggacg ccaatccgca gaaccgccgg gtcgagaacc gcctgccggg
     6481 ggccgacgcc aacccctacc tggccatcgc cgccagcctg ctgtgcggct acgtcggcat
     6541 ggtcgagggc ctgcacccgt cgccgccggt caagggccgc gcctaccagc ggcgcaacct
     6601 gcgcctgccg ctcaacatcg aggcggcgct ggaggccatg gaacagtcga agaccctcga
     6661 gcgctacctg gggcggcgtt tctgccgcgg ctacgtggcg gtcaagcggg tcgagaacga
     6721 gaacttcaaa cgggtgatca gctcctggga gcgcgaattc ctgctgcttt cggtatgagc
     6781 cctcaccccc ttgccaatta agaccggggg aacagcgcaa tgacgcagca acgcactacc
     6841 gcgcagtggc aggcgctcgg ccgcgagcac ctgctggcgc cgttcagcga ctacaagcag
     6901 ctcggcgcca agggcgcgcg catcgtcacc cgggccgacg gcatctacct gtgggacagc
     6961 gagggccagc agatcctcga cgccatggcc ggcctgtggt gcgtcaacgt cggctatggc
     7021 cgccgcgagc tggccgatgc cgcgcaccgg cagatgctcg aactgcccta ctacaacctg
     7081 ttcttccaga ccgcccaccc gccggccatc gagctggccg cggcgatcgc cgagctggcg
     7141 ccggagggca tgaaccacgt gttcttcacc ggctccggct cggaggccaa cgacaccgtg
     7201 ctgcgcctgg tgcgccatta ctgggcgatc aaggggcagc cgcgcaagaa ggtggtcatc
     7261 ggccgggtca atgcctacca cggctccacc gtggccggcg ccagcctcgg cggcatggcg
     7321 gcgatgcacg cgcagggcga cctgccgatc cccggcatcg agcacatcgc ccagccgtac
     7381 tggtacggcg agggcggcgc catggacccg caggccttcg gcgaatgggc cgccaacgag
     7441 ctggagaaga agatcctcga ggtcggcgag gatcgggtgg cggccttcat cgccgagccg
     7501 atccagggcg ccggcggggt gatcatcccc ccggacagct actggccgaa ggtgcgcgcg
     7561 atcctcgcca agtacgacat cctgttcgtg gtcgacgagg tgatctgcgg tttcggccgc
     7621 accggcgagt ggttcggcag ccagtactac ggcctcgagc cggacctgat gtcgatcgcc
     7681 aagggcctga ccagcggcta cctgccgatg ggcggggtga tcgtgcgcga cacggtggcg
     7741 cagaccctgg ccgagggcgg cgagttctac cacggctaca cctactccgg acatccggtg
     7801 gccgccgcgg tggcactgga gaacatccgc atcctgcgcg aggagaagat cgtcgagcgg
     7861 gtcaaacggg aaacggcacc ctatttgcaa cgacgctggc aggagctggc cgaccacccg
     7921 ctggtgggcg aggcccgcgg cctgggcatg ctcggcgcgc tggaactggt caaggacaag
     7981 cagacccgtg cccgcttcga gggccagggc gtcggcatgc gctgccgcga gcactgcttt
     8041 gccaacggcc tgatcatgcg cgcggtcggc gacaccatga tcatctcgcc accgctggtg
     8101 atcaccccca cgcagatcga cgacatgctc ctgctggtgc gcaagtgcct ggacgccacg
     8161 gccgccgaag tgctcggctg agtgacagga cgccgccgcc gagcgtcatc ggcgtgacgg
     8221 cagttgcgcg gcagttattg agcggcgcgc cagtccttgc cagactttgc caccgtgcgt
     8281 tggccagaca ccggagggtg cggatgctga cggcgcaccc cggaagaact cgaaaaaaac
     8341 aggaagcaca aacccatgaa agcattcgcc aagaccctcc tcgccgccac gctggccgcc
     8401 accgtggccg gtgcggcgca ggccgacgac aaggtgctgc acgtctacaa ctggtccgac
     8461 tacatcgccc cgcaaaccgt ggagaagttc gagaagcaga ccggcatcaa ggtggtctac
     8521 gacgtctacg acagcaacga gaccctggag gccaagctgc tggccggcaa gtcgggctac
     8581 gacatcgtgg tgccgtcgaa caacttcctc gccaagcaga tcaaggccgg cgtctaccag
     8641 aagctggaca aggcccagct gtccaactgg cagaacctca acaaggacct gctgaagacc
     8701 ctcgaagtca gcgacccggg caaccagtac gcggcaccct acatgtgggg ctcgatcggc
     8761 atcggcttca atccggacaa ggtcaaggcc gcgctcggcg ccgacgcgcc ggtgaactcc
     8821 tgggatctgc tgttcaagcc ggaaaacatc gagaagctca agggctgtgg cgtcagcttc
     8881 ctcgactcgc cgaccgaaat gctgccgatc gccctgcact acctcggcta caagagcgac
     8941 agccaggatc ccaaggagct gaaggccgcc gaggagctgt tcctgaagat tcgcccgtcg
     9001 gtgacctact tccactcgtc gaagtacatc tccgacctgg ccaacggcaa catctgcgtg
     9061 gccgtcggct actccggcga catcaaccag gccaaggcgc gcgccgagga agccaagggc
     9121 ggcatcaagg tgtcctacaa cattccccag gaaggcgccg gcaccttctt cgacatgctc
     9181 gccatcccgg ccgacgccgg caacccggcc ggcgcgcatg ccttcatgaa cttcctgatg
     9241 cagccggaag tcatggccga aatcaccaac ttcgtgcagt tccccaacgc caacgccgcc
     9301 gccaccgcgc tggtcgacga ggcgatccgc accgaccccg gcgtctaccc gagcgacgag
     9361 gtgatgaaga agctgtacac caccaccgac ctgccggcca aggtgcagcg caccatcacc
     9421 cgcagctgga ccaagatcaa gtcgggcaag taagtagcgc gcggagtccg gccgctcgcc
     9481 gcagcggccg gacagcggtt cgatgcgtgc cgggccgacg ctccgccgca gggcgagggc
     9541 gtcggcctct ttgtttctgc gcagttgccg gctaatgtgc gcgcctcgcg aatcattgcc
     9601 gcccgccggc gccatgccga cgcggcaatc gcaacgcaac gcaacgatca aactgccccg
     9661 gcctgatggc cgggctgccc tgcccccggg agtaccagat ggcgatagcc tccgctgccc
     9721 aaaagaaagc ccagtccggc acccagccgg cccgcgaggt cctggtcaag atcgaccggg
     9781 tgaccaagaa gttcgacgag atggtcgcgg tggatgacgt gtcgctgacc atccacaagg
     9841 gcgagatctt cgccctgctc ggcggctccg gctgcggcaa gtcgaccctg ctgcgcctgc
     9901 tcgccggctt cgagcggccc accgcggggc gcatctacct ggacggccag gacatcaccg
     9961 acctgccgcc ctacgagcgg ccgatcaaca tgatgttcca gtcctacgcg ctgtttccgc
    10021 acatgacggt ggaacagaac atcgccttcg gcctcaagca ggacggcctg cccaaggcgg
    10081 agatcgacgt gcgcgtggcg gagatgctca agctggtgca gatgagccag taagcccagc
    10141 gcaagccgca ccagctgtcc ggcggccagc gccagcgcgt ggccctggcc cgctcgctgg
    10201 cgaaacggcc caagctgctg ctgctcgacg agccaatggg cgcgctggac aagaagctgc
    10261 gctcgcagat gcagctggag ctggtggaga tcatcgagcg ggtcggcgtg acctgcgtga
    10321 tggtcaccca cgaccaggaa gaggcgatga ccatggccga gcgcatcgcc atcatgcacc
    10381 agggctggat cgcccaggtc ggcagcccgg tggacatcta cgaaacaccg gccagccgcc
    10441 tggtctgcga gttcatcggc aacgtcaacc tgttcgacgg cgagctggtc caggacgacg
    10501 ccgaccacgc gatgatcgcc tgtgcgcagc tggagcgacc gatctacatc ggccacggca
    10561 tctccacccg cgccgaggaa aagcacgtca cctacgccct gcgtccggaa aagctgctgc
    10621 tgagcatgga gccgcccgag ctggagcatg ccgactacaa ctggtcgagc ggcaaggtgc
    10681 aggacatcgc ctacctcggc ggccactcgc tgtactacgt cgcgctgccc tcgggcctgg
    10741 tggtgcaggc gttcatggcc aactcggaac gccacgtcaa acggccgacc tgggacgatg
    10801 cggtgtacgt gcactggttc gatgacagcg gcatggtgct gcacgcatga acagtctccg
    10861 ccaactgctc ccgcgcggcc gccacctggt catcggcgtg ccgttcttct ggctgctgct
    10921 gttcttcctg ctgccgttcg ccatcgtgct gaagatcagc ttcgccgaag tcgatgtggc
    10981 gattccgccc tacaccgccc tgctccagta cctcgaccag caactgcagg tggtgctcaa
    11041 cctgggcaac tacctgttcc tcgccgagga cgagctgtac ctggcggcct acctcggctc
    11101 gctgaagatc gccctggtcg gcaccctgct gtgcctgctg atcggctatc cgatggccta
    11161 cgccatcgcc cgcgccagca aagccatgca gacggtgctg ctgctgctga tcatgatgcc
    11221 gacctggacc gcgatcctga tccgcgtcta cgcctggatg ggcatcctcg gcaacaacgg
    11281 cctgctcaac aacctgctgc tcggcctcgg cctgatcgac accccgctgc tgatcctcaa
    11341 taccgacctc gcggtgtaca tcggcgtggt ctacgcctac ctgccgttca tggtcctgcc
    11401 gctgtacgcc aacctggtca agcacgaccc cagcctgctg gaagccgccg ccgacctggg
    11461 cgcgcgccac ctgaccagct tctggaagat caccgtgccg ctgtcgaaga acggcatcat
    11521 cgccggctgc atgctggtgt tcatcccggt ggtcggcgag ttcgtcatcc ccgagctgct
    11581 cggcggcccg gagacgctga tgatcggcaa ggtgctgtgg caggagttct tcaacaaccg
    11641 cgactggccg gtggcctcgg cgctggcggt ggtgatgctg gcgatcctga tggtgcccat
    11701 catcctgttc aacaggaacc aggccaagga actggagggc cgggtatgac gtatgccaat
    11761 gacgtgggcc gatcatcctg ttcaaccggc tttccatcca gaagccgggc caagcaactg
    11821 gagggccggg gatgaacaag cgcttcagtt tctccggctg catgctggtc gccggcctgc
    11881 tgttcatcta cgtgccgatg ctgatcctgg tgctctactc gttcaacagc tcgcggctgg
    11941 tgaccgtgtg gggcggctgg tcgctgaagt ggtatgccgg cctgctcgac aacagccagc
    12001 tgatgggcgc ggtgctgcgc tccctggaga tcgccctgta caccgccatc gccgcgacgg
    12061 cgctgggtac cctggcggcc ttcgtgctca cccgcatccc gcgctttcgc gggcgcaccc
    12121 tgttcggcgg cctggtcacc gcgccgctgg tgatgccgga cgtgatcacc ggcctgtcgc
    12181 tgctgctgct gttcgtggcc atggcccagt tgatcggctg gccggccgag cgcggcctgc
    12241 tgaccatctg gatcgcccac accaccttct gctcgtccta cgtggcggtg gtggtctcgg
    12301 cgcgcctgcg cgaactggac ctgtccatcg aggaggcggc gatggacctc ggggcgcggc
    12361 cgtggaaggt gttcttcctg atcaccgtgc cgatgatcgc gccgtcgctg gcggccgggg
    12421 gcatgatgtc cttcgccctg tcgctggacg acctggtgct ggccagcttc gtctccggtc
    12481 ccggcgccac caccctgccg atggaggtgt tctcggccgt gcggctgggg gtgaagccgg
    12541 aaatcaacgc ggtggccagc ctgatcctgc tggtggtgtc gctgttcacc ttcctcgcct
    12601 ggttcttcag ccaccgtgcc gaggagcgcc gcaagcgcgc catcctgcag gccatggagg
    12661 agatggtgaa ggagcactga gccgtctgtt gccgtccgca acgacaaggg ccacaccgcg
    12721 gggtggccct tgtgcatttt gtagggtgga tggccacccc gtggaaaact cgcgcagcga
    12781 ttttccgcca tgagccgcac acgggtggac aaggctgcgc cgttgtccac cctaccccat
    12841 cctcagtcct gcttgagcag tgccggcagc tgcaccgcca gcttggcgac gttcagcggc
    12901 gcgcggatga agccgcgctg gcggccgtcc gggccgatca cggcgagatt gccgctgtgg
    12961 tcgacggtgt agtgctcgcg gctggtgtcg ccgggaatga aggggatgct caccgcgttg
    13021 gccagcgcct ggatgtccgc cagctcgccg gtcaggccgc tgtagccggc cttgtagtag
    13081 ccgaggtact gcttgagctg cgcgggcgta tcgcgatggg ggtcgatgct gaccatggtc
    13141 acctgcaggc gggccagcgc ctccggctcc agctggctct tgagctggcg cagctcggcc
    13201 agggtggtcg ggcagatgtc cgggcagaag gtgtagccga agaacagcag ggtccaacgg
    13261 cccttgagct gatccacggc ctgcggctgg ccgttctcgt cggtcagggt cagtgccggc
    13321 agcgcgcggc tctgcggcag caggacgatg ccggcgtcgg ccagcgcggc gggatcgctc
    13381 tgcgacggcg cgttgagcac cttgctgaca gtgaggccga gcaccagggc aatggcggcg
    13441 acgaggatga gaacggtctt gtggatgcgg gtcatggact caggttcagc agactgggag
    13501 gcaaataatg atccgccagc aacacgatga acagggcgaa caggtaggta atagagtact
    13561 tgaaggtttt gatcgcagcc tgcggtcggc tgtcacggta cagcgcccag gcccagtcga
    13621 ggaaacgcgc gccgagcagc acggcgccgg ccaggtacag cggcccgctc atgtggatga
    13681 cgaaaggcag caggctgacg gcgaacagga tcagcgtgta gagcaggatg tgcagcttgg
    13741 tgtagtgctc accgtgggtc accggcagca tggggatctc ggccttggcg tagtcatcct
    13801 tgcggtggat ggccagcgcc cagaagtgcg gcggcgtcca ggcgaagatg atcagcacca
    13861 gcagcagcgc ctcggcgtcc agctggccgg tcaccgcggt ccagccgagc agcggcggcg
    13921 ccgccccggc caggccgccg atgacgatgt tctgcggcgt ggcgcgcttc aaaaagcccg
    13981 tgtacagcgc cgcatagccg agcagcgagg ccagggtcag ccaggcggtg agcgcattgg
    14041 tgaaggccag cagcagcgcc tgcccggcca gcgccagcag cagggcgaag gccagcgccg
    14101 cgcgcggcga gacgcgcccg gccaccagcg gacgcaggcg ggtgcgcgcc atgatggcgt
    14161 cgatgcgccg gtctaccacg tgattgactg ccgccgccgc gccggcgcac aggccgatgc
    14221 cgaggttgcc gaacagcagc acgcgccagg gcacgccgct gtcggtggcg aggaacatgc
    14281 cgaccagcgc ggtgatcagc atcagcacca ccaccttggg tttggtcagc tccagatagt
    14341 cgcgccagcc ggcatgggct gggtgctcgt gtaccagcgt ggtcatggcg atccctctcc
    14401 tgttgttgtt ctcggcgcct agcccgtccg gcgcaggctg ccgcgggcga ccaggcgcag
    14461 gccgttatcc atcttcagcg ccaccggcac ccgcagccgg tagttgatca gtaccagcac
    14521 cagcagcagc gtcgcgccgc cgaggttgtg cgccagcgcc acggccagcg gcaggtgcag
    14581 cagcacgttg gccacgccca ggctgacctg cgccagcagc gccactagca gcaggccggc
    14641 cagccgcggc cggccgtggc gcagcagctg cccggccagc accagcagca gcaggctgac
    14701 cagcacggcg ccgaggcgat ggctgaggtg gatggcggtg cgggcctcgc tgtcgagctg
    14761 accgccgaga tagttggggc cgatgtgctg ggtcaggtgg aagccgttgg cgaagtccgt
    14821 cgccggccac cactcgccgt ggcaggtggg caggtcgacg caggccaccg ccgcgtagtt
    14881 gctgctgacc caaccgccca gggcgatctg gccgatcacc acgagcagcg ccagcgccgc
    14941 cagggcgcgc agcggctgca ggacgcctgc cagcggcggc agccggccgg acaggcgcag
    15001 gctgagcagg aacaacaggc tgagggtggc gaagccgccg agcagatggg cactgaccac
    15061 ctgcggccac agcttgaggg tcaccgtcca catgccgaag gcggcctgca acagcaccag
    15121 cgcgaacagc gccagcggca gcttcagcgg ctgaccgtcg cgcccgccgc ggcggatggc
    15181 ctgcacggcg aggacggcga tcagcaggcc caggctaccg gcaaaatagc ggtggaccat
    15241 ctcgttccag cccttctgtg cctccaccgg ggcctcgggg aaatgccgct cggcgtgggc
    15301 cagctgcgct tcgctctgcg gcacgccaag gaagccgtag cagcccggcc agtctgggca
    15361 gccgaggccg gcgtgggtca gccgcgtata ggcgccgagc agaaccacca gcagcgccag
    15421 cgcggtggca cacagggcga gccggtagcc cggtcttgtg gttggcgcca tggcagttcc
    15481 tcccccggtc ctgcacagct gatcagtggc tggcgcagca gacgctcccc ggcatttccc
    15541 gcacctgtct tgaatctagc aggcgcgggc cagcctgccc ggatatcgcc ccgtcagccg
    15601 atctgggaaa tcttcagcag atagcgcaga tcctcgagaa tctgcttgcc gttgctgccg
    15661 gccgggtagc gcagcaccag gttgccgtgc ggatcgacga tccacagctg ggcgcccgcc
    15721 tcgggatagc gctcgccatc cagctccagc aggcgcagct gggggtaggc cgtgtgcagc
    15781 tcggcggcat agtcggccgg cagcgccgca ccgtgggcca ggacatgacc ggcgcgcgag
    15841 acctcgcgat tgaggccgat gtggatctgc cgtgccaggt agaccagctc acgacagtcg
    15901 gcacgacagt cgtcggcact ggtcaccagc agctgccagc gcggctggtc atcgtccacc
    15961 gcgctgcccc aggcgccggc cacgccgaga tcggccgggg tctgcccggt ggcgatcagc
    16021 tcgccgtgat agctgcgagt ctgcggcacc cagaactgcc agcggtacat ggcgctggcc
    16081 agcagcatcg ggcctatcac cacggccagc aggccgagca gttgccagcg cccgcgacca
    16141 gggcgtcgcg caacctgttc aagtgtcaga gtggagccca tcgcgcacct cccgtgcctg
    16201 ctgcacgccg aaccagacaa acaaccccaa cagcgtcgcc gccagggcga accactgcac
    16261 cgcgtagccg agatgctttt ccggccccat gctcaccagc ggccagtcgc ccaggtagct
    16321 ggccggcccg ggctgcagac gcagctcgtc ggccagcccc gcgcgaccca gctcgcgcca
    16381 cagcccggcg gcgtccaccg cgttgacca
//
LOCUS       DATCUN010000120         3182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_211817, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000120 DATCUN010000000
VERSION     DATCUN010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211817"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VIO83_04820"
     CDS             <1..477
                     /locus_tag="VIO83_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8330633.1"
                     /translation="VRYPRGNGPLLAADDSLEPLPIGKAVIRRQGSGVALLVFGVQLA
                     EALQVADKLDATVVDMRFVKPLDQDLVRQLAASHALLVTIEENAIMGGAGSAVSEFLA
                     SEAIVKPLLHLGLPDHYVEHAKPADMLAECGLDAASIEAAVCRRLALLEGNSTQVV"
     regulatory      520..729
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            831..2738
                     /locus_tag="VIO83_04825"
     CDS             831..2738
                     /locus_tag="VIO83_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8330634.1"
                     /translation="MKLSRLALAIALAPGLALAATEQTTELPPLVITRATALHNPTPA
                     SVAVIDRKQIEASASHDLLDVLRTQAGVQIRDTMGDGKRAAISLRGFGENAINNTLVL
                     VDGRRLNQPSLSGPDLNSVPLDNIERIEIIRGAGTVLYGDQAVGGVINIITRTPSRNE
                     AYIETGFGSHDLEAYRGHVFQQLGGGFSAYASGETRNSDNYRDHNNSNYSNAFARLRY
                     DHASGHALYEYQTVDDELLYPNALPLTQRRIDRKQSFSQSWNDTKTQVHRFAVEQRLN
                     NIWSSNFDYSHSDQDGVGSFGPGTEFAQGTRIETFSPRLTARWDTALGRSEWLLGHDH
                     ITSDYQTSWGTDAQQTLRDWYTQLSQPLGHDLTLSVGYRASEVDDNNKGTALKHRDRE
                     GSSSVGLNWQANEQTRLFIKREDVLRWANVDENGFASPGITFLKPQTGESWETGIEWR
                     DGIQRYQASLYRLELEDELMYDPTAIGPYSSFGFDGANINLDKTLRKGLLLEGERQLT
                     ERLSIGGQYSFTDSEYRDGSFQGNDVPWVSRHSASANLGYEILPGLKSHLEAVYTGKR
                     YYSGDDANTQQRAGSYTLLNAALSYDYQQFNSKLRINNLTGKRYDAFASAASRYPAPE
                     EDVQLSVGYRF"
     gene            complement(2801..>3182)
                     /locus_tag="VIO83_04830"
     CDS             complement(2801..>3182)
                     /locus_tag="VIO83_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEY8330635.1"
                     /translation="GVRELAARHARAEPLAVFYQVWDRPMYTLGGRQIISDALRLCGA
                     RNVFGDLDLPAPQVSVESVLARDPAVILVGEPGQAGAWQDWPQLSAVRRGQVWEVPDE
                     GIERPSFQMLAATEKLCKLLDGAR"
BASE COUNT          615 a         1127 c          947 g          493 t
ORIGIN      
        1 gtgcgctacc cgcgcggcaa cggcccgctg ctggccgccg acgacagcct cgagccgctg
       61 ccgatcggca aggccgtaat ccgccgccag ggcagcggcg tcgccctgct ggtgttcggt
      121 gtgcagctgg ccgaagccct gcaggtggcg gacaagctcg acgccaccgt ggtcgacatg
      181 cgcttcgtca agccgctgga ccaggacctg gtgcgccaac tggccgccag ccacgcgctg
      241 ctggtgacca tcgaggaaaa cgccatcatg ggtggcgccg gctcggcagt cagcgagttc
      301 ctcgccagcg aggcgatcgt caagccgctg ctgcacctcg gcctgccgga ccactatgtg
      361 gaacacgcca agccggccga catgctcgcc gagtgcggcc tcgacgccgc cagcatcgag
      421 gccgcggtat gccggcgcct ggcgctgctt gagggaaatt caactcaagt cgtctgaaag
      481 ctgcttgagt tccatgacgc cgcgcactaa ggtgcgcggc gttttgcttt tcgtcagggt
      541 gtgccggcat gggccggcat taaacgggaa gccggtgcgt cctgcggaca atcccggcgc
      601 tgcccccgca acggtaatcg accgcagacc tcggtctgca cgcctgttca acagccactg
      661 ggaatgcccg ggaaggcgaa caggtgcgcg tcgacagccc ggagaccggc cccgacgaag
      721 tcgacaggtg ttgcggaggg ccgcaccgtc agtcacgtgc ccgcctcgcc ggtgcgtcgc
      781 tggttcctgc cctcctcgaa agtctgcctt ccacttttga ggatttcctg atgaagcttt
      841 cccgtctggc cctggccatt gccctcgcgc ccggcctcgc cctggccgcc accgagcaaa
      901 ccaccgagtt gccgccgctg gtcatcactc gcgccacggc cctgcacaac ccgacacccg
      961 ccagcgtcgc ggtgatcgac cgcaagcaga tcgaagccag cgccagccac gacctgctcg
     1021 acgtgctgcg tacccaggcc ggcgtgcaga ttcgcgacac catgggcgat ggcaagcgtg
     1081 ccgccatcag cctgcgcggc tttggcgaaa atgccatcaa caacacgctg gtactggtag
     1141 acgggcgccg cctgaaccag cccagcctgt ccggccccga cctcaacagc gtgccgctgg
     1201 acaacatcga gcgcatcgag atcatccgcg gcgccggcac cgtgctctat ggcgaccaag
     1261 ccgttggcgg ggtgatcaat atcatcaccc gcacgccgtc ccgtaacgaa gcctatatcg
     1321 agactggctt cggcagtcat gacctggagg cctaccgcgg tcacgtgttc cagcaactgg
     1381 gcggcggctt ctccgcctac gccagcggcg aaacccgcaa cagcgacaat taccgcgacc
     1441 acaacaacag caactacagc aacgccttcg ctcgcctgcg ctacgaccat gccagcggcc
     1501 atgcgctcta cgaatatcag acggtcgacg acgagttgct ctaccccaat gcactgcccc
     1561 tgacccagcg tcgtatcgac cgcaaacaga gtttttccca gagctggaac gacaccaaga
     1621 cgcaggtaca ccgctttgca gtcgagcagc gcctgaacaa tatctggtcc tccaacttcg
     1681 actacagcca tagcgatcag gatggtgtcg gcagtttcgg ccccggtacg gagttcgccc
     1741 aaggcacccg gatcgagact ttcagcccgc gtctgaccgc ccgctgggat accgccctcg
     1801 gccgcagcga atggctgctt ggtcacgacc acatcaccag tgactaccaa acctcctggg
     1861 gcaccgacgc ccagcagacc ctgcgcgact ggtataccca actcagccag ccgctgggcc
     1921 atgacctgac cctgagcgtc ggctatcgcg ccagcgaagt ggatgacaac aacaaaggca
     1981 ccgccctcaa gcaccgcgac cgcgaaggca gcagcagcgt tggcttgaac tggcaagcca
     2041 atgagcagac ccgcctgttc atcaagcgcg aggatgtatt gcgctgggcc aacgtcgatg
     2101 aaaacggttt cgcctcgccc ggcattacct tcctcaagcc gcagaccggc gagtcctggg
     2161 aaaccgggat cgaatggcgc gatggcatcc agcgttacca ggccagcctg taccgtctcg
     2221 agctggaaga cgagctgatg tacgacccga ccgcaatcgg cccctacagc agctttggct
     2281 tcgacggcgc caacatcaac ttggacaaga ccctgcgcaa gggcctgctg cttgagggtg
     2341 aacgccagct gacggaacgc ctgagcattg gcggccagta cagcttcacc gactccgagt
     2401 accgtgacgg cagtttccag ggcaacgacg tgccctgggt atcgcgccac agcgccagtg
     2461 ccaacctcgg ctacgaaatc ctgcccggcc tgaagagtca cctggaagcg gtgtacaccg
     2521 gcaaacgcta ttactcgggg gatgatgcca atacccaaca gcgagccggt agttacaccc
     2581 tgctcaatgc ggctctcagc tacgactacc agcagttcaa cagcaagctg cggatcaaca
     2641 acctgactgg caagcgctat gacgcctttg ccagtgccgc cagccgctac ccggcgccgg
     2701 aagaggatgt gcagctgagc gtcggctacc gcttctgagg ttgatcgcaa aacgacgagg
     2761 ccccgcatcc gcggggcctc gtcgttcttg gccgcaaaaa tcagcgcgcc ccatccagca
     2821 acttgcacaa cttctcggtg gcggcgagca tctggaagct cggccgctcg atgccctcgt
     2881 ccggcacttc ccagacctgg ccgcggcgca ccgcgctcag ctgcggccag tcctgccagg
     2941 cgccggcctg gcccggctcg ccgaccagaa tcaccgccgg gtcgcgggcc agcaccgact
     3001 cgacgctgac ctgcggcgcc ggcaggtcga gatcgccgaa cacgttgcgc gcgccgcaca
     3061 ggcgcagggc atcgctgatg atctgccggc cgccgagggt atacatgggc cgatcccaga
     3121 cctgatagaa caccgccagc ggctcggccc gcgcgtggcg cgcggccagc tcgcgcacgc
     3181 cc
//
LOCUS       DATCUN010000121         4671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_249913, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000121 DATCUN010000000
VERSION     DATCUN010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249913"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..98
                     /locus_tag="VIO83_04835"
     CDS             <1..98
                     /locus_tag="VIO83_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330636.1"
                     /translation="EEGFNDLRTSGLLKAMQGMTSLAEVNRVTKD"
     gene            101..1318
                     /locus_tag="VIO83_04840"
     CDS             101..1318
                     /locus_tag="VIO83_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003116250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8330637.1"
                     /translation="MAAKPLQNSTFVWEGTDRKGAKLKGEISGLNPNLVKAQLRKQGI
                     NPLKVKKKAGSLLGKGKAIKPLDIALFTRQMATMMKSGVPLLQSFDIIADGVENQNMR
                     ALVLEVKQEVAAGNSLAASLRKKPLYFDDLYCNLVDSGEQAGALETLLDRIATYKEKT
                     EALKAKVKKAMTYPIAVLVVALIVSAILLIKVVPQFQDVFANFGAELPAFTQMVINLS
                     EVVQEWWFIGLIGIIGSLYAFKAAHQRSEKLRNKVDRILLKLPLIGDITYKSAVARFA
                     RTLSTTFAAGVPLVEALDSVSGAAGNVVFRDAVNRVKQDVTTGMQLNFSMRSTGLFPA
                     MAVQMTSIGEESGSLDSMLDKVAEYYEAEVDNMVDNLTTLMEPMIMAVLGVLVGGLII
                     AMYLPIFQLGAVV"
     gene            1322..2194
                     /locus_tag="VIO83_04845"
     CDS             1322..2194
                     /locus_tag="VIO83_04845"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEY8330638.1"
                     /translation="MNIAELLASNTLALVFCITIIGLLVGSFLNVVVYRLPIMMQRDW
                     QSQAREVLELEHEEPGERFNLLLPDSSCPHCGHQIRAWENIPVISWLLLRGRCSGCKA
                     PISKRYPLVELACGVLSGYVAWHFGFTWQAGAMLLLSWGLLAMSLIDADHQLLPDSLV
                     LPLLWLGLIVNQSGLFASPGDAFWGAVAGYLSLWSVYWLFKLLTGKEGMGYGDFKLLA
                     MLGAWGGWQILPLTILLSSVVGAVLGLIILRMRSAESGTPLPFGPYLAIAGWIALLWG
                     DEITGSYLQFAGMN"
     gene            2348..2962
                     /gene="coaE"
                     /locus_tag="VIO83_04850"
     CDS             2348..2962
                     /gene="coaE"
                     /locus_tag="VIO83_04850"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425004.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY8330639.1"
                     /translation="MKPWILGLTGGIGSGKSAAASHFASLGVHLVDADQAARWVVEPG
                     RPALAHIVDRFGEQILQDSGQLDRAALRTRIFQAPEERQWLEQLLHPLIREEIRNVLD
                     EARSPYAILVSPLLVESAAQREMARRVLVVDVPEQVQIQRTVQRDQVPEEQVRAILQA
                     QASREERLRHAHDVLVNDRDLAWLQREVERLHEFYLTLEGGRPQ"
     gene            2959..3159
                     /gene="yacG"
                     /locus_tag="VIO83_04855"
     CDS             2959..3159
                     /gene="yacG"
                     /locus_tag="VIO83_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEY8330640.1"
                     /translation="MKKPLTVACPTCATPVEWSTDNPYRPFCCERCKLIDLGAWAAEE
                     HKIPGDPLEDELFSGELDGLRH"
     gene            complement(3186..3869)
                     /locus_tag="VIO83_04860"
     CDS             complement(3186..3869)
                     /locus_tag="VIO83_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009867982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEY8330641.1"
                     /translation="MITAALLSESSLWLGALLFLPLLLAALWQAPWVELFSDFRRQHL
                     LFGTVLALFLLWLVRRDFPSGLSYHFIGMTAVTLLLDWPLAVLAAFVAQLGIAALGRQ
                     DWLALGVNGVLLILVPVLVAEVCARLVERLQPRNLFVYIFFSGFFPAALTAVLVVLAG
                     LGVLLIDGIYQMPPWLDDFAGYLWLIMFPEAFINGMVVTALVVFYPDWLETFNRTRYL
                     QAPWKDEER"
     gene            complement(3866..4333)
                     /locus_tag="VIO83_04865"
     CDS             complement(3866..4333)
                     /locus_tag="VIO83_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007970535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-containing dehydrogenase"
                     /protein_id="HEY8330642.1"
                     /translation="MKRWMLLGLALLAATVQALETGERLAPWTLSDQFDQPYTLDDRL
                     HVLLVARSMDGAKLLGAALEGRPKGYLEARQALFVADISRMPSLIGRLFAIPAMRDYS
                     YRVLLDREAQVVPQYAVPQEGVLWLQLEKGVLRERKVFTDSAALRAALEQVAP"
     gene            complement(4330..>4671)
                     /locus_tag="VIO83_04870"
     CDS             complement(4330..>4671)
                     /locus_tag="VIO83_04870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003161766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8330643.1"
                     /translation="EDAYVPFCAYLARNPGLSLLAEEEGAIVGCLLAGHDGRRGYLQH
                     LLVDPRCRQRGIATALIEAVLARLATQGIAKSHVFVLDAAPQALTFWRSRAGWSERED
                     IRVFSTVEHNR"
BASE COUNT          899 a         1515 c         1477 g          780 t
ORIGIN      
        1 gcgaggaagg cttcaacgat ctgcgcacct cgggcctgct caaagccatg caaggcatga
       61 ccagcctcgc ggaagtcaac cgtgtgacca aggattaacc atggcggcca aaccgttaca
      121 gaacagcacc ttcgtctggg agggcaccga ccgcaagggc gccaagctca agggggagat
      181 cagcgggctg aaccccaatc tggtcaaggc ccagctgcgt aaacagggaa tcaaccccct
      241 caaggtgaag aagaaggccg gctcgctgct gggcaagggc aaggcgatca agccactgga
      301 tatcgccctg ttcacccgac agatggcgac catgatgaag tccggagtgc cgctactgca
      361 gtcgttcgac atcattgccg acggcgtgga aaaccagaac atgcgcgccc tggtccttga
      421 ggttaagcag gaagtcgccg ccggcaacag cttggcggcc tctctgcgca aaaagccgct
      481 gtatttcgac gacctctact gcaacctggt ggactccggt gaacaggctg gcgcgctgga
      541 aaccctgcta gaccggatcg ccacttataa agaaaagacc gaggctctga aggccaaggt
      601 caagaaggcg atgacctacc cgatcgcggt actggtggtg gcgctgatcg tctcggctat
      661 tttgctgatc aaggtggttc cgcagtttca ggatgtattc gccaacttcg gcgccgaact
      721 accagccttt acccagatgg tgatcaatct gtcagaagtg gtgcaggaat ggtggttcat
      781 cgggctgatc gggattatcg gctccctcta tgcgttcaag gctgctcacc agcgctcgga
      841 aaagcttcgc aacaaggtgg accgcatcct gctgaaactg ccactgattg gcgatatcac
      901 ctacaagagt gcggtggcgc gctttgcacg caccctgtcc accaccttcg ccgccggcgt
      961 accgctggtg gaggcactgg actccgtttc cggcgccgcc ggcaacgtgg tgttccgcga
     1021 tgcggtgaac cgcgtcaaac aggacgtaac caccggcatg cagctgaact tctccatgcg
     1081 ctccacgggc ctcttcccgg ccatggccgt gcagatgacc tcgatcggcg aggagtccgg
     1141 ctcgctggac agcatgctcg acaaggtggc cgagtactac gaggccgaag tcgacaatat
     1201 ggtcgacaac ctgaccacgc tgatggagcc gatgatcatg gccgtgctcg gcgtgctggt
     1261 cggcggcctg atcatcgcca tgtacctgcc gatcttccag ctgggcgccg tcgtctgaat
     1321 catgaatatt gccgagcttc tggccagcaa tacgctggct ctggtttttt gcatcaccat
     1381 catcggtctg ctggtcggca gcttcctcaa cgtggtggtc taccgcctgc cgatcatgat
     1441 gcagcgcgac tggcaatccc aggcgcgtga ggtgctcgaa ctggagcacg aggagcctgg
     1501 cgagcgcttc aacctgctgc tgccggactc cagctgtccg cactgcgggc atcagatccg
     1561 tgcctgggaa aatatcccag tcatcagttg gctgctcctg cgcggccgct gctcgggctg
     1621 caaggcgccg atcagcaagc gctatccgct ggtggaactg gcctgcggcg tgctttccgg
     1681 ctacgtggcc tggcatttcg gctttacctg gcaggccggc gccatgctgc tgctgagctg
     1741 ggggctgctg gcgatgagcc tgatcgatgc cgaccatcaa ctgctaccag actccctggt
     1801 gctgccgctg ctgtggctgg ggctgatcgt caaccagtcg gggctgttcg cctcgccggg
     1861 cgatgccttc tggggcgcgg tggccggcta tctcagcctg tggtcggtgt actggctgtt
     1921 caagctgctg accggcaagg aaggcatggg ctacggcgac ttcaagctgc tggcgatgct
     1981 cggcgcctgg ggcggctggc agattctgcc gctgaccatc ctgctgtcgt cggtggtcgg
     2041 cgccgtgctt ggcctgatca tcctgcgcat gcgcagtgcc gaaagcggca ccccgctgcc
     2101 cttcggcccc tatctggcca tcgctggctg gattgccctg ctgtggggcg acgagatcac
     2161 cggcagctac ctgcagtttg ccgggatgaa ctgaactttc cgccgcataa aatatcgcca
     2221 gcaagctgac tccaacagcc ggagccagct tgctggcgat ctgcaccttc ctcagccgaa
     2281 tccgtgactg gcaccccgta gcctcgacgg cgataatgcg cccatcgcag caacggatct
     2341 tcctcccatg aaaccctgga ttctcggcct caccggcggc atcggcagcg gcaagagcgc
     2401 ggcggccagc catttcgcca gcctcggcgt gcatctggtg gatgccgatc aggcggcgcg
     2461 ctgggtggtg gagccagggc ggccggcact ggcgcatatc gtcgaccgct tcggcgagca
     2521 gattctccag gacagcggac agctggaccg cgcggccttg cgcacacgca tcttccaggc
     2581 gccggaggag cgccagtggc tggagcagct gctgcaccca ctgatccgcg aggaaatccg
     2641 caacgtgctc gacgaggcac gctcgcctta cgccatcctg gtgtcgccgc tgctggtgga
     2701 gtccgctgcg cagcgcgaga tggctcgccg cgtgctggtg gtggatgtgc ctgagcaggt
     2761 gcaaatccag cgtaccgtgc aacgtgacca ggtgccggaa gagcaggttc gcgccatcct
     2821 gcaggcccag gccagccgcg aggaacgcct gcgccacgcc cacgacgtgc tggtaaacga
     2881 ccgcgatctg gcctggctgc agcgcgaggt ggagcgcctg cacgaattct atctgaccct
     2941 tgaaggaggc cgacctcaat gaaaaagccg ctgaccgtag cctgtccgac ctgcgccact
     3001 ccggtggaat ggagcaccga caacccttac cggccgttct gctgcgagcg ctgcaagctg
     3061 atcgacctcg gcgcctgggc cgccgaggaa cacaagattc ccggcgatcc gttggaggac
     3121 gagctgttca gcggcgaact ggacgggttg cggcactgat cagatcagcc ggcgcagccc
     3181 ccaccctagc gctcctcgtc cttccacggc gcctgcaggt agcgggtgcg gttgaaggtt
     3241 tccagccagt ccggatagaa caccaccagc gcggtaacca ccatgccgtt gatgaaggcc
     3301 tcgggaaaca tgatcagcca caggtagccg gcgaagtcgt cgagccaggg cggcatctgg
     3361 tagatgccat cgatcagcag cacgccgagg ccggcgagca caaccagcac cgcggtcagc
     3421 gccgccggga agaagccgct gaagaagatg tagacgaaca ggttgcgcgg ctgcaggcgc
     3481 tcgaccaggc gcgcacagac ctcggcgacc agcaccggga ccaagatcaa cagcacgccg
     3541 ttcaccccca gagccagcca gtcctggcga ccgagggctg caatccccag ctgagcaacg
     3601 aaggcggcga gcactgccag cggccagtcg agcagcaggg tcaccgcggt catgccgatg
     3661 aagtggtagg acaatccgga aggaaagtcg cgccgtacca gccagagcag gaacagcgcc
     3721 agcacggtgc cgaacagcag gtgctggcgg cggaagtcgc tgaacagttc gacccagggc
     3781 gcctgccaga gcgctgccag cagcagcggc aggaacagca gggctcccag ccacaggctg
     3841 gactcggaca gcagcgccgc agtgatcacg gcgcaacctg ctccagcgcc gcgcgcagcg
     3901 ctgccgaatc ggtgaacacc ttgcgctcgc gcagcacacc cttctccagt tgcaaccaga
     3961 gcacgccctc ctgcggaacg gcatattgcg gcaccacctg cgcctcgcga tccagcagca
     4021 cgcgatagct gtagtcgcgc atcgccggaa tggcgaacag cctgccgatc aggctgggca
     4081 tgcgactgat atcggccacg aacagcgcct gacgggcctc cagataaccc ttgggccgcc
     4141 cttccagcgc cgcgccgagc agcttggcgc catccatgct gcgcgccacc agcaggacgt
     4201 gcagccggtc atcgagggtg tagggctggt cgaactggtc gctaagggtc caaggcgcga
     4261 gacgctcgcc cgtttccaag gcttgtacgg tagcagccag cagggccagg cctagcaaca
     4321 tccagcgttt catcgattgt gctccacagt ggaaaagact cgaatatcct cacgctcact
     4381 ccagccagca cggctgcgcc agaaggtcag cgcctgcggc gcggcgtcga ggacgaagac
     4441 atgactcttg gcgatgccct gtgtcgccag gcgggccagc accgcttcga tcagcgccgt
     4501 ggcgatgccg cgctgccggc aacgcggatc gaccagcagg tgctgcaggt agccgcgccg
     4561 cccatcatgg ccggccagca ggcagccgac aatcgccccc tcctcctcgg ccagcagact
     4621 gagcccggga ttgcgcgcca ggtaggcgca gaacggcaca taggcatcct c
//
LOCUS       DATCUN010000122         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_61337, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000122 DATCUN010000000
VERSION     DATCUN010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61337"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /gene="dmpG"
                     /locus_tag="VIO83_04875"
     CDS             complement(<1..710)
                     /gene="dmpG"
                     /locus_tag="VIO83_04875"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HEY8330644.1"
                     /translation="MALKDLKGRKVTLHDMCLRDGMHAKRQQITLEQMTAIASALDEA
                     GVPMIEVTHGDGLDGTSINYGFGLHTDEEYLKATVPHLKQAKVSCLLVPGIGTVDGLR
                     MAADLGVSCIRVATHCTEADCGEQHIGLARKLNLDAVGFLMMAHMVSPEKILEQAKLF
                     ESYGATCIYVTDSAGYMLPDDVTAKVGLLRANLAPNVELGFHAHNNLSMSVANSLAAI
                     DAGANRIDGSAAGLGAGAG"
     gene            complement(726..1643)
                     /locus_tag="VIO83_04880"
     CDS             complement(726..1643)
                     /locus_tag="VIO83_04880"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017849234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HEY8330645.1"
                     /translation="MKINCALIGSGNIGTDLMFKLKRSPVLNPVWMVGIDPDSDGLRK
                     AREAGMKTTHEGVDGLLPHLAEDNIQIAFDATSAYVHKENSDKLTSRGVVMIDLTPAA
                     IGPFCVPPVNIKDHLGKREMNVNMVTCGGQATIPMVYAVSRVQPVVYGEIVATVASKS
                     AGPGTRKNIDEFTRTTSKGIEVVGGARKGKAIIILNPAEPPLIMRDTIHCLTETVPDE
                     DAIRASVTDMIKEVQKYVPGYRLLHNIVFDGNRVSIFVEVEGLGDYLPKYAGNLDIMT
                     AAGTRTAEMIAEDIIAGSFNPAQYKTAAA"
     gene            1838..>1965
                     /locus_tag="VIO83_04885"
     CDS             1838..>1965
                     /locus_tag="VIO83_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8330646.1"
                     /translation="MSDNPYHCRRFEGQTAVVTGAAQGIGRATAQRLAAEGARVIVA"
BASE COUNT          290 a          632 c          683 g          360 t
ORIGIN      
        1 ccggcgccgg cgcccaggcc cgccgccgag ccatcgatgc ggttggcgcc ggcatcgatg
       61 gccgccagcg agttggcaac cgacatcgac aggttgttgt gggcgtgaaa gcccagttcg
      121 acgttcggcg ccaggttggc gcgcagcagg ccgaccttgg ccgtgacgtc gtccggcagc
      181 atgtagccgg ccgagtcggt cacgtagatg caggtggcgc cgtaggactc gaacagtttg
      241 gcctgctcga ggattttttc cgggctgacc atgtgcgcca tcatcaggaa gccgaccgca
      301 tccaggttca gcttgcgggc taggccaatg tgctgctcgc cgcagtcggc ctcggtgcag
      361 tgcgtggcca cgcggatgca ggacacgcca agatcggccg ccatccgcag gccgtccacg
      421 gtgccgatgc ccggcaccag caggcaggac accttggcct gcttcaggtg cggcacggtc
      481 gccttcaggt actcctcgtc cgtgtgcagg ccaaagccgt agttgatgga ggtaccgtcc
      541 aggccgtcgc cgtgggtgac ctcgatcatc ggcacgccgg cctcgtccag cgccgaggcg
      601 atggcggtca tctgttccag ggtgatctgc tggcgcttgg cgtgcatgcc gtcgcgcagg
      661 cacatgtcgt gcagggtgac cttgcggcct ttcaagtctt tcagggccat ggcgatgtcc
      721 tcaggtcagg cggcggcggt cttgtactgc gccgggttga agctgccggc gatgatgtct
      781 tcggcaatca tctcggccgt gcgggtgccg gcggcggtca tgatgtccag gtttccggcg
      841 tacttgggca ggtagtcgcc caggccctcg acctcgacga agatcgacac ccggttgccg
      901 tcgaacacga tgttgtgcag caggcggtag ccgggcacgt acttttgcac ctctttgatc
      961 atgtccgtca ccgaggcacg gatggcatcc tcgtcgggga cggtctcggt caggcaatgg
     1021 atggtgtcgc gcatgatcag cggcggctcg gccgggttca ggatgatgat cgccttgccc
     1081 ttgcgggcgc cgcccaccac ctcgataccc ttggacgtgg tgcgggtgaa ctcgtcgata
     1141 ttcttgcgcg tgccggggcc ggccgacttg gaggccacgg tggcgacgat ctcgccgtac
     1201 accaccggct gcacgcggct gaccgcgtag accatcggga tggtggcctg gccgccgcag
     1261 gtgaccatgt tcacgttcat ctcgcgcttg cccaggtgat ccttgatgtt caccggcggc
     1321 acgcagaacg ggccgatggc ggccggcgtg aggtcgatca tcaccacgcc gcggctggtc
     1381 agcttgtcgg agttttcctt gtgcacgtag gccgaggtgg cgtcgaaggc aatctggatg
     1441 ttgtcttccg ccaggtgcgg cagcaggccg tccacgccct cgtgggtggt cttcatgccg
     1501 gcctcgcgcg ccttgcgcag gccgtcggag tcggggtcga tgccgaccat ccagaccgga
     1561 ttgagcaccg ggctgcgctt taacttgaac atgaggtcgg taccgatgtt gcccgacccg
     1621 atcagggcgc agttgatctt catttgaggc tcctcggcag gcctgggaac ggccggaatg
     1681 ccgtcgcccg gcggcgagcg gcccggatgg cacggtccgg accggtaatg gtacttgccg
     1741 gctgccgtgg gccacaaggc aggcactttg cgcgacccgg cagtcgcagc atactcgctg
     1801 gcccacaccc agccgccgcc tgccagagga gccacgcatg agcgacaacc cctaccactg
     1861 ccgccgtttc gagggtcaga ccgccgtggt caccggcgcc gcgcagggca tcggacgcgc
     1921 cacggcgcag cgcctggcgg ccgagggcgc acgggtgatc gtggc
//
LOCUS       DATCUN010000123         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_24040, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000123 DATCUN010000000
VERSION     DATCUN010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24040"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..967
                     /locus_tag="VIO83_04890"
     CDS             80..967
                     /locus_tag="VIO83_04890"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase"
                     /protein_id="HEY8330647.1"
                     /translation="MEKIKGALVVGALRLVAALPWSWVQGLGAAIGWLMWRLPNRSRE
                     VVRINQAKCFPELGAAEREALLGRTLKDIGKTFTESACAWIWSPQKSLALVKEVEGLE
                     VLEAALASGKGVVGITSHLGNWEVLNHFYCNQCKPIIFYRPPKQKAVDELLQQQRVQM
                     GNRVAPSTKEGILSVIKEVRRGGAVGIPADPEPSEGSGLFVPFLGVQALTSKFVPNML
                     AGHKAVGVFLHAIRLDDGSGFKVILEAAPEDMYSKDPEVGVAAMSRVLESYVRRWPSQ
                     YMWSMKRFKKRPPGEPRWY"
     gene            1183..2358
                     /locus_tag="VIO83_04895"
     CDS             1183..2358
                     /locus_tag="VIO83_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330648.1"
                     /translation="MNSLKGPLATDSLAVDWPLASLFHGPAMSLLRALIVALLVALTG
                     CASRERPPAPPPPVVVAESTWRQIDSDIFAASQEATGEATGYARDLMQRWRGLVYQRS
                     EAEFIPWFSSYWTRQWLTMKVTWYQLNAGDEKEAVVDRLALYLQEQYQERVLDPVAKQ
                     VSPDWIMAQTTQLYVRLLREQLQGIAPRYAVPVDQFATHLQGIPAIALAPPASLDASL
                     YQLVSAEPIDSQPAYVALVERLQQARGGPGDWSADAGISSLARQTSKSLVAERSRGVA
                     GTVSSMVGRVAGTVISLGIAGFSALVRENERPKMEAQLRNNLNVAFDEEWLDLLHNPA
                     SGVLAGVNHIAGQIEGGLADSVLQPVDVEPVLPTVVMPGEQPRQDEKNDDETLYRLW"
BASE COUNT          404 a          820 c          894 g          408 t
ORIGIN      
        1 gtgtgcaccc ggtcgccagc gtggccggcg gcggccgcgc gggtacaatg gcccccctgt
       61 ccaatcgcgg gagagtcctg tggagaagat caaaggtgcc ctggtggtgg gtgcgctgcg
      121 gctggtcgcc gccctgccct ggtcctgggt gcagggcctc ggcgcggcca tcggctggct
      181 gatgtggagg ctgcccaacc gctcgcgcga ggtggtgcgc atcaaccagg ccaagtgctt
      241 ccccgagctg ggcgcggccg aacgcgaggc gctgctcggg cgcaccctga aggatatcgg
      301 caagaccttc accgagagcg cctgcgcgtg gatctggtcg ccgcagaagt cgctggcgct
      361 ggtcaaggag gtcgagggcc tggaggtgct cgaggcggcg ctggccagcg gcaagggcgt
      421 ggtcggcatc accagccacc tgggcaactg ggaggtgctc aaccacttct actgcaacca
      481 gtgcaagccg atcatcttct accggccgcc caagcagaag gcggtggatg agctgctgca
      541 gcagcagcgg gtgcagatgg gcaatcgggt ggcgccgtcg accaaggaag gcatcctcag
      601 cgtgatcaag gaggtgcgcc gcggcggtgc tgtgggcatt cccgccgacc cggagccgag
      661 cgaaggcagc ggcctcttcg tgccgttcct cggcgtgcag gcgctgacca gcaagttcgt
      721 gcccaacatg ctcgccgggc acaaggcggt cggcgtgttc ctgcatgcca tccgcctcga
      781 cgacggcagc ggcttcaagg tgatcctcga ggcggcgccg gaggacatgt acagcaagga
      841 cccggaggtc ggtgtggcgg cgatgagccg ggtgctcgag agctacgtgc ggcgctggcc
      901 gagccagtac atgtggagca tgaagcgctt caagaagcgc ccgccgggcg agccgcgctg
      961 gtactgaggg cgggccgctg caaccgccgg ccccggagcg ccgccactgc atctgatcgg
     1021 aggatatgcc ggcgccgtct tgcgcctcct atactcaaga caccgtggtc ggacgtgctg
     1081 gacgttccgt tctagaggct ggcaaacctg cgcaggctcc gcatgcagtt tgttaggagg
     1141 aagagagcag ccctttgagg tctgcggagc ctttgagccc ccatgaacag cctgaagggt
     1201 ccgcttgcaa cagattcgct ggcggttgac tggccgctgg cgagtctgtt ccacggacct
     1261 gcgatgtcgc tcctgcgcgc cctgatcgtc gccctgctgg tggcgctgac ggggtgcgct
     1321 tctcgcgagc gcccacccgc cccgcctcct ccggtggtgg ttgcggaaag tacctggcgg
     1381 caaatcgaca gcgacatctt cgccgcgtcg caggaggcga ccggcgaggc cacgggttac
     1441 gcccgcgacc tcatgcagcg ctggaggggc ctcgtctacc agcgcagcga ggccgagttc
     1501 attccgtggt tttccagcta ctggacccgg caatggctga ccatgaaggt gacctggtat
     1561 cagctgaacg ccggggacga gaaggaggcg gtggtggatc gcctggcgct ctatctgcag
     1621 gagcaatacc aggagcgcgt gctggatccc gtcgccaagc aggtcagtcc cgactggatc
     1681 atggcgcaga cgacgcaact ctacgtccgc ctgctgcgtg agcaactgca gggcattgcg
     1741 ccgcgctacg ctgtccctgt ggaccaattc gccacccacc tgcagggcat tcccgccatc
     1801 gcgctggcgc cgccagccag cctcgacgcg tcgctctacc agctggtcag cgccgagccg
     1861 atcgacagtc agccggccta tgtggccctg gtcgagcgcc tccagcaggc ccgtggtggc
     1921 ccgggagact ggtcggcgga cgccggcatc tcgtcgctgg ccaggcagac gagcaagagc
     1981 ctggtggccg aacgcagccg cggtgtcgca ggaactgttt cctcgatggt cggccgggtc
     2041 gccggcacgg tgatttctct ggggatcgcg ggtttcagcg ccctcgtgcg tgaaaacgaa
     2101 cggccgaaaa tggaggcgca gctgcgcaac aacctcaacg tcgcctttga cgaagagtgg
     2161 ctggacttgc tgcacaaccc cgccagcggg gtgctggcgg gggtcaatca catcgccggg
     2221 cagatcgagg gcggcctcgc cgacagcgtc ctgcagccgg tcgacgtcga gccggttctg
     2281 cccaccgtgg tcatgcccgg cgagcaaccg cggcaggatg aaaagaacga cgacgagacg
     2341 ctctaccgac tgtggtagcg cgcctgcatg actcggcacg gccgtcagcg gcggttacgc
     2401 cggccgcggc ttgtccagct tgttgaggaa taccgtcatc tccttctccg cctgcttgtc
     2461 gccgtggcgg cgggcggcgt ccaggccctg ctgccaggcg ctgcgcgccg cctcggcggc
     2521 gccgag
//
LOCUS       DATCUN010000124         6628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_230563, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000124 DATCUN010000000
VERSION     DATCUN010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230563"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..95
                     /locus_tag="VIO83_04900"
     CDS             <1..95
                     /locus_tag="VIO83_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEY8330649.1"
                     /translation="DRDKASNYGMQLKRLYPGTPEYQQYLAEQK"
     gene            92..1105
                     /locus_tag="VIO83_04905"
     CDS             92..1105
                     /locus_tag="VIO83_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ family helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY8330650.1"
                     /translation="MTMSSHTEAGAAPQRGNPGEVLRLARENKGWQVAEVATQLNLTA
                     QALTQLEAGEFDRLPGHTFARGYIRAYAKLLGLEQAALVEQFDRYTGTDATGSTVHSL
                     GHVPEPLRLSRTVLRLASAVLLLLLLLLGYFWWQERPERLADLGALGLQHIEVESADG
                     TTEIHPLDEPEDQAVAKALEESEPAPLELTPATTGEAPNSAPVNAPAVPELSPVAPAA
                     TTLPAPAPAVAAATAAVPAAVETPPAAPAAPTAPVTVAAGEGLVQVNFSADCWVQVTD
                     ANGRVLVSVVKHKGETLQVTGKAPLDLRLGFARGAQVTYNGAPVNLRDVTSGGTARVK
                     LGQ"
     gene            1107..2216
                     /gene="ispG"
                     /locus_tag="VIO83_04910"
                     /gene_synonym="gcpE"
     CDS             1107..2216
                     /gene="ispG"
                     /locus_tag="VIO83_04910"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY8330651.1"
                     /translation="MHCESPIKRRQSRQIFVGKVAVGGDAPISVQSMTNTDTCDVAAT
                     VAQIRRLEDAGADIVRVSVPDMDAAEAFGRIKQQVQVPLVADIHFDYRIALRVAELGV
                     DCLRINPGNIGREDRVRAVVDAARDKGIPIRIGVNAGSLEKDLQKKYGEPTPEALVES
                     ALRHVEHLDRLDFQNFKVSVKASDVFMAVAAYRLLAAQIEQPLHLGITEAGGLRSGTV
                     KSAVGLGMLLAEGIGDTIRVSLAADPVEEIKVGFDILKSLRLRSRGINFIACPSCSRQ
                     NFDVVKTMNELETRLDDLLVPLDVAVIGCVVNGPGEAKEAHVGLTGGTPNLIYIDGKP
                     VHKLDNDHLVDELEKLIRRKAAEKAAADASLIVRS"
     gene            2226..3515
                     /gene="hisS"
                     /locus_tag="VIO83_04915"
     CDS             2226..3515
                     /gene="hisS"
                     /locus_tag="VIO83_04915"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY8330652.1"
                     /translation="MSKNIQAIRGMNDILPEQTPLWRYLENSVAGLLDGYGYRQIRMP
                     IVEFTDLFKRSIGEVTDIVEKEMYTFDDRNGDSLTLRPEGTASCVRAVQEHGLIGGGQ
                     PQKLWYIGPMFRHERPQKGRYRQFHQIGVELFNFDGPDVDAELIVLTWRLWGVLGIRD
                     AVQLELNSLGSSADRARYREALVEYLSARFEQLDEDSQRRLGSNPLRILDSKNPDTQA
                     LLVDAPKLADYLDDDSRAHFAGLKARLDAAGIPYVLNPKLVRGLDYYGKTVFEWTTDQ
                     LGSQGTVCAGGRYDGLVEQLGGKPTPAVGFAMGIERLVLLIETLDKVPAELARQVDVY
                     VAAFGEAAELAALTLSEQLRDAVPGLRLAVNAGAGSFKSQLKKADKSGALFALILGED
                     ELAQQVVALKPLRGEGQQQTIAWDALAAHLAACLAQA"
     gene            3542..4189
                     /locus_tag="VIO83_04920"
     CDS             3542..4189
                     /locus_tag="VIO83_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEY8330653.1"
                     /translation="MTSRTEEEELAVIKDWWQRNGKPLLTGSVLALVGVFGWQAWQKH
                     ETNQTYAASSVYQQLVDVALNTAGQPDAGKVSELAGKLNKDFAGSHYAQYASLLLARV
                     AVDSGRLDEAAAELRKVLDKPVDATLGEVARQRLARVLAAQDKAEEALALLAGDAAPA
                     FVAGREELRGDLLVKLGRSSEARSAYEKARAALPQEAGLGTLQIKLDDLTEGETR"
     gene            4186..5328
                     /gene="bamB"
                     /locus_tag="VIO83_04925"
     CDS             4186..5328
                     /gene="bamB"
                     /locus_tag="VIO83_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEY8330654.1"
                     /translation="MMRWTHALMLATAVLAAGCSSNSTKELPPAELTKFSEEVRLDKQ
                     WSRSIGEGQGETWNQLELASDGDSLYAADIEGLVVAMNRETGKVLWEQELDKPVSGGV
                     GAGYGLVLVGTLKGQVIALDSATGEVKWQAPVGSEVLSAPANNGDVVVVQTQDDHVFG
                     LDAATGERRWSYESTPAVLTLRGTSAPVVTNRLAVAGLSSGKVVALDIQRGIPVWEQR
                     VAMAKGRSELERMVDIDGALLLSGGTLYVVTYQGQLAALDLQSGRPLWQRDASSYVGV
                     AAGFGNVYVSQASGTVEGIDERSTSALWSNDALARRQLSTPVAFSSNVAVGDLEGYLH
                     LLSQVDGRFVGRERVDSDGLRARPLVVGDWLYAYGNSGKLVAYTLR"
     gene            5568..>6628
                     /gene="der"
                     /locus_tag="VIO83_04930"
     CDS             5568..>6628
                     /gene="der"
                     /locus_tag="VIO83_04930"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY8330655.1"
                     /translation="MVPVIALVGRPNVGKSTLFNRLTRSRDAIVADYSGLTRDRKYGE
                     AKWQGRTYIVIDTGGISGDEEGIDAKMAEQSLQAIEEADAVLFMVDSRAGMTAADQMI
                     AEHLRKKNKCTFLVANKVDTVDPDIARAEFSPLAIGDAWPIAAAHGRGITALLQAALG
                     EFPKDEVEEEEFPEGEGEEGGAAAEPKKRIPGPDERTGIKIAIIGRPNVGKSTLVNRM
                     LGEERVIVYDQAGTTRDSIYIPFERDDAKYTLIDTAGVRRRGKIFEAVEKFSVVKTLQ
                     AIQDANVVVFVMDAREGVVDHDLNLLGFVLETGRALVIALNKWDGMDSHERDRVKTEL
                     ERRLQFVDFADIHFISALHG"
BASE COUNT         1158 a         2279 c         2220 g          971 t
ORIGIN      
        1 aggaccgcga caaggcctcc aactacggca tgcagctcaa gcgcctgtat ccgggcacgc
       61 ctgaatatca gcaatacctt gcggagcaaa aatgaccatg tcgtcgcata ccgaagcagg
      121 cgcagcgccg cagcggggca acccgggtga agtcctgcgt ctggctcggg aaaacaaggg
      181 ctggcaggtg gccgaggtgg ctacccagct caacctgacc gcgcaggccc tgacgcaact
      241 ggaagccggc gaattcgacc gtctgcctgg gcataccttt gcccgcggct acatccgcgc
      301 ctatgccaag ctgctgggcc tggagcaggc ggcactggtc gaacagttcg atcgctacac
      361 cggtaccgat gccaccggca gtaccgtgca tagtctcggt cacgtgcccg agccgctgcg
      421 cctgtcgcgc accgtgctgc gcctggccag cgcggtgctg ctgctgctgt tgctgctgct
      481 cggctacttc tggtggcagg agcgccccga gcgcctcgcc gacctcggtg ccctggggct
      541 gcagcacatc gaagtggaaa gtgccgacgg caccaccgag atccatccgc tggacgagcc
      601 ggaagaccag gccgtggcca aggccctcga agagagcgag ccggcgccgc tcgagctgac
      661 tccggcaacc accggcgaag cgccgaactc cgcgccggtc aacgccccgg ccgtgcccga
      721 gctgtcgccg gtcgccccag cggccaccac actcccggct ccggctccgg ctgttgcggc
      781 cgccactgcc gctgtgccgg ctgccgtcga gactccgcct gccgcgcccg ccgcaccgac
      841 tgcgccggtc acggtcgccg ccggcgaagg cctggtgcag gtcaacttca gcgctgactg
      901 ctgggtccag gttaccgacg ccaatggccg ggtgctggtc agcgtggtca agcacaaggg
      961 cgaaaccctg caggtgaccg gcaaggctcc gttggacctg cgcttgggct ttgcccgcgg
     1021 cgcccaggtg acctacaacg gtgcgccggt caatctgcgc gacgtcacct cgggcggtac
     1081 cgcccgcgtc aagttggggc agtaagatgc attgcgaatc tccgatcaag cgccgccagt
     1141 cgcggcagat cttcgtcggc aaggtggcgg tcggcggtga tgcaccgatc tcggtgcaga
     1201 gcatgaccaa caccgacacc tgcgacgtcg ccgccaccgt ggcgcagatc cgtcgcctgg
     1261 aagacgccgg cgccgacatc gtgcgcgtct cggtgccgga catggacgcc gccgaagcct
     1321 tcggccgcat caagcagcag gtgcaggtgc cgctggtcgc cgacatccac ttcgactacc
     1381 ggatcgccct gcgcgtcgcc gaactgggcg tcgactgcct gcgcatcaac ccgggcaaca
     1441 tcggccgcga ggaccgcgtg cgcgcggtgg tggatgccgc ccgcgacaag ggcattccga
     1501 tccgcatcgg cgtcaacgcc ggttcgctgg aaaaggacct gcagaagaag tacggcgagc
     1561 cgacccccga ggcgctggtc gaatccgccc tgcgccacgt cgagcatctc gaccgcctgg
     1621 acttccagaa cttcaaggtc agcgtcaagg ccagcgacgt gttcatggcc gtcgccgcct
     1681 accgcctgct ggccgcgcag atcgagcagc cgctgcacct gggcatcacc gaggccggcg
     1741 gcctgcgttc cggcaccgtg aagtcggcgg tgggcctggg catgctgctc gccgagggca
     1801 tcggcgacac catccgcgtg tcgctggccg ccgatccggt ggaagaaatc aaggtcggct
     1861 tcgacatcct caagtcgctg cgcctgcgct cgcgtggcat caacttcatc gcctgcccga
     1921 gctgctcgcg gcagaacttc gacgtggtca agaccatgaa cgagctggaa acccggctcg
     1981 acgatctgct ggtgccgctc gacgtggccg tgatcggctg cgtggtcaac ggcccgggcg
     2041 aagccaagga ggcccacgtc ggcctcaccg gcggcacgcc gaatctgatc tacatcgacg
     2101 gcaagccggt acacaagctg gacaatgacc atctggtgga cgagctggaa aagctgatcc
     2161 gccgcaaggc ggcagagaag gctgcggccg acgccagcct gatcgtccgc agttaaggaa
     2221 ttcgcgtgag caagaacatc caggccatcc gtggcatgaa cgacatcctg ccggaacaga
     2281 ccccgctctg gcgctacctc gagaacagcg tggccggcct gctggacggt tacggctacc
     2341 ggcagatccg catgccgatc gtcgagttca ccgacctgtt caagcgctcc attggtgagg
     2401 tcactgacat cgtcgagaag gagatgtaca ccttcgacga ccgcaacggc gactcgctga
     2461 ccctgcgccc ggaaggcacc gcctcctgcg tgcgcgcggt gcaggagcac ggcctgatcg
     2521 gcggcggcca gccgcagaag ctgtggtaca tcggcccgat gttccgccac gagcgcccgc
     2581 agaagggccg ctaccgccag ttccaccaga tcggcgtgga gctgttcaac ttcgacggcc
     2641 cggacgtcga cgccgagctg atcgtgctga cctggcgcct gtggggcgtg ctcggcatcc
     2701 gcgatgcggt gcagctcgag ctcaacagcc tgggcagcag cgccgaccgt gcgcgctacc
     2761 gcgaggcgct ggtcgagtac ctctccgccc gtttcgagca gctcgacgag gacagccagc
     2821 gtcgcctggg cagcaacccg ctgcgcatcc tcgacagcaa gaatcccgac acccaggccc
     2881 tgctggtgga tgcgcccaag ctggccgact acctggatga cgactcgcgc gcccacttcg
     2941 ccggcctcaa ggcccgcctg gacgccgccg gcattcccta cgtgctcaac cccaagctgg
     3001 tgcgcggcct cgactactac ggcaagaccg tgttcgagtg gaccaccgac cagctcggct
     3061 cgcagggcac cgtgtgcgcc ggcggtcgct acgacggcct ggtcgagcag ctgggcggca
     3121 agccgacccc ggcggtcggt ttcgccatgg gcatcgagcg cctggtgctg ctgatcgaga
     3181 ccctggacaa ggtgccggcc gagctggccc gccaggtcga cgtctatgtc gccgccttcg
     3241 gcgaagccgc cgaactggcc gcgctgacgc tctccgagca gctgcgcgat gccgtgccgg
     3301 gcctgcgcct ggcggtcaac gccggtgccg gcagcttcaa gagccagctg aagaaggccg
     3361 acaagagcgg cgcgctgttc gctctgatcc tcggcgagga cgaactggcg caacaagtgg
     3421 tcgcgctcaa gcccctgcgt ggcgaaggcc agcagcaaac cattgcctgg gatgcgctgg
     3481 ccgcgcatct cgccgcttgc ctggcgcaag cctgaacctg accaattaag ggagtgacca
     3541 ggtgacctcg cgtaccgaag aagaagaact ggcggtaatc aaggactggt ggcagcgcaa
     3601 cggcaagccc ctgctcaccg gcagcgtgct ggcgctggtc ggcgtattcg gctggcaggc
     3661 ctggcagaag cacgagacca accagaccta cgccgcctcg agcgtgtacc agcagctggt
     3721 ggacgtcgcc ctcaacaccg ccggccagcc ggatgccggc aaggtcagcg agctggccgg
     3781 caagctgaac aaggacttcg ccggcagcca ctatgcccag tacgccagcc tgctgctggc
     3841 gcgggtggcg gtggacagcg gtcgtctcga cgaggccgcc gccgagctgc gcaaggtact
     3901 ggacaagccg gtcgacgcga ccctgggcga agtcgcccgt cagcgtctgg cccgcgtgct
     3961 ggccgcgcag gacaaggccg aggaggccct ggccctgctg gccggcgacg ccgcgccggc
     4021 gttcgtcgcc ggtcgcgagg agctgcgcgg cgacctgctg gtcaagctcg gccgcagcag
     4081 cgaggcgcgc agcgcctacg agaaggcgcg cgcggccctg ccgcaggagg cgggtctcgg
     4141 tacgctgcag atcaaactgg acgacttgac tgaaggggag acccgatgat gcgctggact
     4201 catgcactaa tgctggcgac ggcggtgctg gccgccggtt gcagcagcaa ctcgaccaag
     4261 gaactgccac cggccgaact gaccaagttc agcgaagaag tccgcctgga caagcagtgg
     4321 agccgctcca tcggtgaagg ccagggggaa acctggaacc agctggagct ggcgtcggac
     4381 ggcgacagcc tgtacgccgc cgacatcgaa ggcctggtcg tggcgatgaa ccgcgaaacc
     4441 ggcaaggtgc tgtgggagca ggaactggac aagccggtct ccggtggcgt cggtgccggt
     4501 tacggcctgg tgctggtcgg caccctcaag ggccaggtga tcgccctcga cagcgccacc
     4561 ggcgaggtca agtggcaggc gccggtcggc agcgaggtgc tctccgcgcc ggccaacaac
     4621 ggcgacgtgg tggtggtgca gacccaggac gaccatgtgt tcggtctgga tgccgcgact
     4681 ggcgagcgcc gctggagcta cgaaagcacg ccggcggtgc tgaccctgcg cggcaccagc
     4741 gcgccggtgg tgaccaaccg cctggcggtg gccggtctgt ccagcggcaa ggtggtcgcc
     4801 ctcgacatcc agcgcggcat cccggtgtgg gaacagcgcg tggccatggc caagggccgt
     4861 tccgaactgg agcgcatggt cgacatcgac ggcgccctgc tgctgtccgg cggcaccctg
     4921 tacgtggtga cctaccaggg tcaactggcg gcgctggatc tgcaaagcgg ccgcccgttg
     4981 tggcagcgcg atgcctccag ctacgtcggc gtggccgccg gcttcggcaa cgtctacgtc
     5041 agccaggcca gcggcaccgt cgaaggcatc gacgagcgct cgacctcggc gctgtggagc
     5101 aacgacgccc tggcgcgtcg ccagctgtcg accccggtgg cgttctccag caacgtcgcg
     5161 gtcggcgacc tggaaggcta cctgcacctg ctgagccagg tggacgggcg cttcgtcggc
     5221 cgcgagcgcg tcgacagcga cggcctgcgc gcccgcccgc tggtggtcgg cgactggctg
     5281 tacgcctacg gcaacagtgg caagctggtg gcctacaccc tgcgctgaga gccagcccag
     5341 gatctgctgc gcgtcggcca tgctgcgttg aaatcggctg cggactgctc atttacagcc
     5401 cgtaaactcc gcgtcctcag ccgatttcgc cttgcctggc tctagctcgc cagatcctga
     5461 acaggcctac ggagtgccca cggaggcact cgacatgctc atatcgcggc cgctgcatct
     5521 acactgcagc ggccgttgtg tttttccgaa ttaccgtgga gagccgcatg gttcccgtaa
     5581 ttgctctggt aggacggccg aacgtcggca agtccaccct gttcaaccgc ttgacccgca
     5641 gccgcgacgc catcgtcgcc gactactccg gcctgacccg cgaccgcaag tacggcgagg
     5701 cgaagtggca ggggcgcacc tacatcgtca tcgacaccgg cggcatctcc ggtgacgagg
     5761 aaggcatcga cgccaagatg gccgagcagt cgctgcaggc catcgaggaa gccgatgccg
     5821 tgctgttcat ggtcgactcg cgcgccggca tgaccgccgc ggaccagatg atcgccgagc
     5881 acctgcgcaa gaagaacaag tgcaccttcc tggtcgccaa caaggtcgat accgtcgacc
     5941 cggacatcgc ccgcgccgag ttcagcccgc tggccatcgg cgacgcctgg ccgatcgccg
     6001 ccgctcacgg tcgcggcatc acggcgctgc tgcaggccgc gctgggcgag ttccccaagg
     6061 acgaagtcga ggaagaggag ttccccgagg gtgaaggcga ggagggcggc gccgcggccg
     6121 agccgaagaa gcgcatcccc ggcccggacg agcgtaccgg gatcaagatc gccatcatcg
     6181 gccgccccaa cgtcggcaag tcgaccctgg tcaaccgcat gctcggcgaa gagcgggtca
     6241 tcgtctacga tcaggccggc accacccgcg acagcatcta cattcccttc gagcgcgacg
     6301 acgccaagta caccctgatc gacaccgccg gcgtgcgccg ccgcggcaag atcttcgagg
     6361 cggtggaaaa gttctcggtg gtcaagaccc tgcaggcgat ccaggacgcc aacgtggtgg
     6421 tcttcgtcat ggacgcccgc gagggtgtgg tcgaccacga cctcaacctg ctcggcttcg
     6481 tgctggaaac cggccgcgcg ctggtgatcg cgctgaacaa gtgggacggc atggacagcc
     6541 acgagcgcga ccgggtcaag accgagctgg agcgccgcct gcagttcgtc gacttcgccg
     6601 acatccactt catctccgcc ctgcacgg
//
LOCUS       DATCUN010000125        18046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_136169, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000125 DATCUN010000000
VERSION     DATCUN010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 18046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18046
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136169"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VIO83_04935"
     CDS             <1..712
                     /locus_tag="VIO83_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEY8330656.1"
                     /translation="VAQAYSARKGRSGAPRLCHDLREAGLPCNRKTVAASMQRQGLRA
                     KAARKFKATTNSRHSLPVADNLLKQDFTASAPNQKWVGDITYLHTEEGWLYLAVVIDL
                     YSRMVIGWAMGERMTADLVCDALRMALWRRKQPRSVIVHTDRGSQYCSAAYQELIRAH
                     HLRCSMSAKGNCYDNACAESFFHSLKVECVHGEHFRSRTQMRETVFEYIETDYNRQRR
                     HSTLGHISPEAFEARMSA"
     gene            complement(755..2152)
                     /locus_tag="VIO83_04940"
     CDS             complement(755..2152)
                     /locus_tag="VIO83_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY8330657.1"
                     /translation="MSALPASPAPSPFRRSLSEVRALLALAGPIIVAQLANTAMGFVD
                     TVMAGRVGPRDLAAVALGNSIWVPMLLLMGGILLATTAKVSRKVGAGTYALIGPLVRQ
                     ALWLALAIGLIAAALLFCAEPILVLMQVEASLRTTTMGYLQAVALGFPALALYQVLRG
                     FSDGLGSTRPTMVIGLLGLLLNIPLNYMFIYGKFGLPAMGGVGCGWATALVMWFMLGA
                     LLWWVHWAPLYKSSRLFERLEAPQWPAIRALLTLGLPIGIAIFAEASIFSVIALLIGS
                     LGASVVAGHQVALNFSSMVFMIPYSLAMATTVRVGQALGRGAPRDARFSAGVAMATAL
                     VYACLSASLMLLLRADIARLYTPDTEVLAVASALLMYSALFQFSDAIQVTAAGALRGY
                     HDTRATMLITLLAYWGIGLPIGYGLGLADWFGPASGPAGLWQGLVVGLTCAALLLGVR
                     LQRSARRHIRAVHTA"
     gene            2280..3422
                     /gene="pdxB"
                     /locus_tag="VIO83_04945"
     CDS             2280..3422
                     /gene="pdxB"
                     /locus_tag="VIO83_04945"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017148870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HEY8330658.1"
                     /translation="MRLLADENIPLLDPFFAAFGEIRRLPGRAIDRAAVADADALLVR
                     SVTRVDRALLDGSPVKFVGTCTIGTDHLDLLYFVEAGIAWSSAPGCNARGVVEYVLGA
                     LFALAERRGADLAARTYGVVGAGQVGSRLVEVLRGLGWKVLVCDPPRQAREGGDFVGL
                     EEIIRRCDVISLHTPLTRDGDDATWHLFDVARLAQLKPGCWLINASRGAVVDNAALKA
                     HLQGGIDLDVVLDVWEGEPQVDVELAALCRLATPHIAGYSLDGKLRGTEQIYQAFCAW
                     RGEQAQVELAQLLPPAWLGELVLHEQADPAWALAMVCRAVYDPRLDDAAFRRSLLADT
                     EQRRAGFDALRKHYPMRRELPDLTVRVSGAAQQLRRQLAALGVRLA"
     gene            complement(3423..3605)
                     /locus_tag="VIO83_04950"
     CDS             complement(3423..3605)
                     /locus_tag="VIO83_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009404578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330659.1"
                     /translation="MHTPLHIAHSRQHTAQKLRHLQLGAIIDAQGREIPITEQMILQA
                     CRQLEQDAKPFRRLFG"
     gene            complement(3694..4944)
                     /locus_tag="VIO83_04955"
     CDS             complement(3694..4944)
                     /locus_tag="VIO83_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8330660.1"
                     /translation="MLKRQAYQFRIEPNGEQQRKLRQFAGMARFVFNKALALQTETRE
                     AGGKHLNYAAMSRQLTTWRNSDETPWLKDAPVHPLQQSLKDLDRAYQNFFAKRADFPR
                     FKKRGKHDSFRYPDAKQFKVDQANSRIFLPKLGWMRYRNSREVLGTLKNLTVSLRAGQ
                     WYVSIQTEREVEIPRHPSALAVGIDLGVVRFATLWDGQRETVLEPLNSFAAHQHRLAK
                     AQRQMARKTKFSRNRLKAKAKVQRIQLQIANARNDYLHKATHTLSQNHALVCVEDLRV
                     GNMTRSASGTAEQPGRNVRQKSGLNRSILDQGWGEFRRQLDYKATWCGGWLEAVPPQY
                     TSQHCTACGYTHAENRQRQAEFLCQACGFEENADVVGAMNIRERGLKQLEGQDHAQIA
                     CEVNGAIRPSAAGTHRSVHAATAA"
     gene            complement(5184..5465)
                     /locus_tag="VIO83_04960"
     CDS             complement(5184..5465)
                     /locus_tag="VIO83_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HEY8330661.1"
                     /translation="MFKVNEYFNGTVKSIGFEMTEGPATVGVMAPGEYEFGTAKAEVM
                     HVIAGALTVKLPGSDAWETFAAGSKFDVPADSKFQLQVAQDTAYLCEYR"
     gene            5628..7901
                     /locus_tag="VIO83_04965"
     CDS             5628..7901
                     /locus_tag="VIO83_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015636082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HEY8330662.1"
                     /translation="MAIRALLATVLLLLGGVLQAQPVSYSRDIQPILTQKCVACHACY
                     DAPCQLNLGSGAGIQRGASPQAVYNGKRTEAQATTRLFVDAQGEQGWRTRGFHSVLGG
                     GEAALVARMLELGRSHAPLPGSKLPEDLDIGIRRENQCPRPDQFDDFARKNPGAGMPF
                     AVTGLSDGEYATLRRWLVQGAPLDGEPLEPNASESAQIAEWEALFNRPGAREQLVARW
                     LYEHLFLAHLYFDAGKSGHFFELVRSRTPPGELVDVIATRRPNDDPGTHFFYRLRPIQ
                     GVIVHKTHITYALNPRKQARIETLFFAADWQVDAVPGYGPQRRANPFATFAAIPAQAR
                     YQFMLDDAEYFVRTFIRGPVCRGQIATDVIRDHFWTLFQAPAHDLYLTDAAYRERVTP
                     LLALPGVQDGLAQLPLLWHESKANRNAYEAARREAYSHTPYPSWSHIWAGNDNALLSI
                     FRQLDSASVRKGLIGAVPQTLWWLDFPLLERTYYQLVVNFDVFGNVSHQAQTRLYFDL
                     IRNGAEQNFLRLMPANQRQAILGDWYQDGGKLKLWLDYEGIDADTPSGLRLPADDPKA
                     AFAERLLQRYGSLNARPDPLNRCRDERCFRPGASPALQRMDQRLSVLAARPLSQLPAI
                     EYLPEATLLRVELDGGGREVYSLLRNRAHSNVAFLLGESLRYQPEKDTLTLYPEVLSS
                     YPNFLFSLREGEVNAFVAALQQARSAADFEMIVRRWGVRRTHPQFWALFHDLGEHIRE
                     HEPVEAGVLDMNRYQNL"
     gene            8022..8606
                     /gene="nfuA"
                     /locus_tag="VIO83_04970"
     CDS             8022..8606
                     /gene="nfuA"
                     /locus_tag="VIO83_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HEY8330663.1"
                     /translation="MSNITITESAQEYLADLLQKQNTPGIGIRVFITQPGTQYAETCI
                     AYCKPSEQKAEDTPLALKDFTAWLDAVSVPFLEDAVVDYATDRMGGQLTIKAPNAKVP
                     MVNEDSPLPERINYYLQTEINPGLASHGGMVSLVDIDDEGVAILRFGGGCQGCGMVDM
                     TLKDGVEKTLMERIPELKGVRDVTDHSNKENAFY"
     gene            8777..10078
                     /locus_tag="VIO83_04975"
     CDS             8777..10078
                     /locus_tag="VIO83_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003173624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8330664.1"
                     /translation="MHKSMLRRLLVVDLFDEFNPIAPEVRSAGWIVDLCSLDEAFDRQ
                     ADVGLIRLGPCLDSGGKLLRRTPMQWIAALPPDISLDGSLGLFVGEWCFDFHTLPFDR
                     PRLLVTLGHALGMAALRRAEQVDGGDELLGDAPCMRELRKLLGKLGPTDSPVLIRGES
                     GTGKELVAHALHRLSRRAEQAFVAINCGAIPEQLIQSELFGHEKGAFTGAHQRKIGRI
                     ESAHGGTLFLDEVGDLPLDLQANLLRFLQEGQIERVGGNQPIEVNVRVLAATHIDLEQ
                     AVERGRFREDLYYRLNVLQVHTSPLRERLEDVPALARHFARLYADEVGRRPRSFSEEA
                     LRAMGEYAWPGNVRELANRVRRGLVLAEGQQIMAQDLGFRGVCVPTSLDYSLENYKMQ
                     AEKQALSDVLRRYSANLSHAARALGVSRPTFYRLLNKHQIR"
     gene            complement(10154..11398)
                     /locus_tag="VIO83_04980"
     CDS             complement(10154..11398)
                     /locus_tag="VIO83_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003144432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY8330665.1"
                     /translation="MLQSHSLRVAVGIAALLPSTLLYAASDAELRELQQALLELRQRY
                     DAQQKALMVLEQRVREVETQPAVPQPQRLARSPSSTSQPAAGYGESLREDNTPARSVE
                     NIYDKASGFFGEGTFSLETGLTYSHYDTRQLGLNGLLLLDAIFLGNINVDQINSDNFT
                     LDMTGRYNLGNRWQFDLNVPLVYRESTYESAGAGGSTTQVSSESVTGDPRLGDVSFGV
                     AYKFLDESESRPDTVFSLRVKAPTGEDPYGIKLVQSPDNDNLSVPEDLPTGNGVWSVT
                     PGISVVKTVDPAVLFGNLSYTYNLEESFDDISPVQNQKVGGKVKMGNWFQYGLGTAFA
                     LNERMSLSLSYSQLISQKSKIKPDGGSWQTVSSSDANAAYFNIGMTYALSDKLTMVPN
                     LSIGLTPDSPDFSFSLKFPYYF"
     gene            complement(11443..12174)
                     /locus_tag="VIO83_04985"
     CDS             complement(11443..12174)
                     /locus_tag="VIO83_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330666.1"
                     /translation="MKIHVLLAASCIAASICAQASNPLQPVELSDQELSQLRGRFVMP
                     GHIVHFGVTMSSVWENASGQVLAGAVHMQASEGMFQPQFNVSTITQNGDQSAPAAGSG
                     QIYGGEGLSTVNGVTQSVRSAGDFNSAANNFVVNVKRGGSAPNAGNAGQPFANQIEST
                     QAGNVQISASDGGFRIAINALGQGTSLQQLAGGGLQQHANIGGSSNMVRNLTELDVVL
                     RDTPIPASSMGVNLDQLRALRPAGY"
     gene            complement(12229..12909)
                     /locus_tag="VIO83_04990"
     CDS             complement(12229..12909)
                     /locus_tag="VIO83_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEY8330667.1"
                     /translation="MRLAILPVLCCLSTALQAAQVPFAVLPGGAIAFKHVESIRERRF
                     SNLVEQKTDFSCGAAALATILREAYRFDVDETFVINGMLAGADHELVRTQGFSMLDMK
                     RYVENLGMRARGYRVPAAGLEQLKVPAIVLQEVRGYKHFVVLQRTENGWAYIGDPIFG
                     HKRYKVEDFLKGWNGIVFAVIGREYDKTNALLNPPKPLSAKDRLNAFQPVKDAELMEH
                     GFIQSDFF"
     gene            complement(12976..14325)
                     /locus_tag="VIO83_04995"
     CDS             complement(12976..14325)
                     /locus_tag="VIO83_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HEY8330668.1"
                     /translation="MKVSMALKPLVFAMAAAMAMAAQANQSNGHNHDHDPLLDRNAGD
                     ATAYAGRDQYIGDAQVTNEGTENSVNIENSLSSSGNIGANVAAGSMNQQANNVAIATA
                     DEAFVFGSAEADSTIDQDNDGWTYNLGGSNAATVNGSGNDASGNIGVNAASGNLNQQQ
                     NALAIATARGWDATATATGNQDLRGGVDNKAASVTVETTFDVTKDWSNNYSKAESSDS
                     SDSWDFSWQAQSAKSATASASLSASHDSSESSDRTYNASLDVVVDANKSSSSSSEWDK
                     TAHLDATASASLDGHLVVSPGRIHAHGDGALEIDANANAAENASEESAAHFDLAKSVD
                     ASSSYTADDSRSVDKSFEASFEKSSEASASASAEKNESHSANSLIEEAGNSTLAATVT
                     FTKSYTFANPVINSATLSDSLNNASGNIGVNIASGSSNQQLNTLAVAVGCNSCNNGN"
     gene            complement(14384..14956)
                     /locus_tag="VIO83_05000"
     CDS             complement(14384..14956)
                     /locus_tag="VIO83_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008040271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEY8330669.1"
                     /translation="MLRLTLLIAMTIPVASLAEQAQVNNHATIDNSGRTYQGVLSVNQ
                     AAGSQQQQINGRALAIGADATTNFRQYQELDGVDTSMNAQANITGSAFSNGSGVLGVN
                     QSAGAGTQQINSFRMSVGSAAQSVDDSILAQQNVVLAQNSGTAETKPGERAVAIDRQA
                     FAGSSGVVQLNQSAGVGNRMANTVSIRVMD"
     gene            complement(14958..15416)
                     /locus_tag="VIO83_05005"
     CDS             complement(14958..15416)
                     /locus_tag="VIO83_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330670.1"
                     /translation="MDRNASRISRPALLAMLMLSAMGAAIPAHSADGIIVITRDVQPR
                     VATRPALLPDPNPRTVNANPSSTVIRATNSFELTDADFARVTSGSSLQRQMLPSGQLP
                     GLSNSSQASSHGLPGMRAGQSGGAGSNVSNQINRSLQQGLAPLKILTGDR"
     gene            15769..16236
                     /locus_tag="VIO83_05010"
     CDS             15769..16236
                     /locus_tag="VIO83_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HEY8330671.1"
                     /translation="MHPFTAPRPPHLAWLLGLAGLIPFISGALGIWITPVGWRPLVLT
                     ALLDYAAVILAFMGAIHWGLAMRAEQDDLRAQLQLGLSVIPPLLGWAAVSFGLPAALA
                     IPVFVLAFVGLYMADLRAVRLGLAPLWYRPLRMPLTVVVVACLLIAWAGVLAG"
     gene            complement(16238..16849)
                     /locus_tag="VIO83_05015"
     CDS             complement(16238..16849)
                     /locus_tag="VIO83_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330672.1"
                     /translation="MSTINFQEVNLDKKPNLDLIWDLDRLEQRELAQRFIRLFENRLC
                     VYSESVSQLYTNYGLHFPAELGRKMVVLPNPYAFHDTLNHISPYSVRKTGLCVLPGQF
                     QGHNGLLLARLGAQGEMLQARPFKSALAQIISKLKQNGDVFLPVLMKGDLREFDQRMP
                     YLHLHRLQLTQLPHLSAFERNDLQQTVTRKLLMLYRQADQLSC"
     gene            17213..18046
                     /locus_tag="VIO83_05020"
     CDS             17213..18046
                     /locus_tag="VIO83_05020"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY8330673.1"
                     /translation="MEFWTAIQALILGIVEGLTEFLPISSTGHQIIVADLIGFGGDRA
                     KAFNIIIQLGAILAVVWEFRQRIFGVICGLPRERQAQRFTANLLIAFFPAVILGLAFA
                     DLIEHWLFNPITVASALVIGGVVMLWAEKRQHAIEVDTVDDMSWKHALKIGCAQCLAM
                     IPGTSRSASTIIGGLIFGLSRKAATEFSFFLAMPTMVGAAVYSGYKHRALFENGADLP
                     VFALGFVTSFVFAMLAVRALLKFIASHSYAVFAWYRIGFGLLILATWQLGMIDWSTAQ
                     G"
BASE COUNT         3036 a         5569 c         5970 g         3471 t
ORIGIN      
        1 ggtcgcgcag gcatacagcg cacgcaaggg ccgcagcggg gctccacggc tgtgccacga
       61 tctgcgggaa gcagggctgc cctgcaatcg caagacggtt gccgcctcga tgcaacggca
      121 aggactgcgc gccaaggccg ccaggaagtt caaggccacg acaaactccc ggcactcgct
      181 gccggtggca gataacctgc tcaagcagga tttcacggca tcggcaccga accagaaatg
      241 ggtgggtgac atcacctacc tgcacacgga ggaaggctgg ctgtatctgg cggtggtcat
      301 cgacctgtac tcacggatgg tgatcggctg ggctatgggc gagcgcatga cagccgacct
      361 ggtctgcgat gccttgcgca tggcgctgtg gagacgcaag cagcccagga gtgtgatcgt
      421 gcataccgac cgcggcagcc agtactgctc agcggcttac caggagctga tccgtgccca
      481 tcacttgcga tgcagtatga gcgccaaggg caactgctat gacaatgcct gtgcggagag
      541 cttctttcac agcctgaagg tcgagtgcgt ccacggcgag cacttcagga gccgcaccca
      601 gatgcgtgaa accgtgtttg aatacatcga aaccgactac aaccgccagc gccgccacag
      661 cacgctgggg cacatcagcc cggaggcctt cgaggcccgg atgagtgctt aaacccgtgt
      721 ccacagctgc tgggcaagat caggcgacgc aggctcacgc tgtgtggact gcacggatat
      781 gccgtcgcgc gctgcgctgc aggcgcacgc cgagcaacag ggcggcgcag gtcaggccca
      841 ccaccagccc ctgccacagt ccggccgggc cgctggccgg cccgaaccag tcggcgaggc
      901 ccagaccgta gccaatcggc aggccgatgc cccagtaggc cagcagggtg atcagcatgg
      961 tcgcgcgggt gtcgtggtag ccacgcaggg caccggcggc agtgacctgg atggcgtcgg
     1021 agaactggaa cagcgccgag tacatcagca gcgcgctggc cacggcgagt acctcggtgt
     1081 ccggggtgta caggcgggcg atgtccgcgc gcagcagcag catgaggctc gcggacaggc
     1141 aggcatagac cagcgccgtg gccatcgcga cacccgcaga gaagcgcgca tcacgcggcg
     1201 ccccgcgccc cagcgcctgg ccgacccgta ccgtggtggc catggccagc gagtagggga
     1261 tcatgaacac catcgaggaa aagttcagcg ccacctgatg cccggccacc acgctggcgc
     1321 ccaggctgcc aatcagcagg gcgatcaccg agaagatgct ggcctcggcg aaaatggcga
     1381 tgccgatcgg caggccgagc gtcagcagcg cgcggatcgc cggccactgc ggcgcttcca
     1441 ggcgctcgaa cagccggctg gacttgtaca gcggcgccca gtgcacccac cacagcaggg
     1501 cgccgagcat gaaccacatc accagcgcgg tggcccagcc gcagccgacg ccgcccatgg
     1561 ccggcaggcc gaacttgccg tagatgaaca tgtagttgag cgggatgttc agcaacaggc
     1621 cgagtagacc gatcaccatg gtcggccggg tgctgcccag accgtcgctg aagccgcgca
     1681 gcacctggta cagcgccagc gccgggaagc cgagcgccac ggcctgcagg tagcccatgg
     1741 tcgtcgtgcg cagcgaggcc tccacctgca tcagcaccag gatcggctcg gcgcagaaca
     1801 gcagcgcggc ggcgatcagg ccgatggcca gcgccagcca cagcgcctgg cgcaccagcg
     1861 ggccgatcag cgcgtaggtg ccggccccta ccttgcgcga caccttggcg gtggtcgcca
     1921 gcaggatgcc gcccatcagc agcagcatcg gtacccagat cgagttgccg agcgccacgg
     1981 cggccagatc gcgcgggccg actcggccgg ccatcacggt atcgacgaag cccatggcgg
     2041 tgttggcgag ctgcgcgacg atgatcgggc cggcgagggc gagcagggcc ctgacttcgc
     2101 tgaggctacg acggaagggg gagggggcgg gagaagcggg cagggcggac atcgacaaac
     2161 ctcggcaagc agcccgcaag tctacgccgt gcaccagagt ggggccagcg gagtgccgcg
     2221 ccggatcggc cgatcaggta aagtggcggc aactcccgat cttccgacgg atactgccca
     2281 tgcgcctgct ggccgacgaa aacattccgc tgctcgatcc tttctttgct gcgttcggcg
     2341 agattcgccg cctgcctggc cgggccatcg accgcgccgc cgtggctgat gccgatgcgc
     2401 tgctggtgcg ctcggtgacc cgcgtcgacc gtgcgctact tgacggcagc ccggtgaagt
     2461 tcgtcggcac ctgtaccatc ggcaccgacc atctggacct gctgtatttc gtcgaagccg
     2521 gtatcgcctg gtccagcgct cccggctgta acgcccgcgg ggtggtcgag tacgtgctcg
     2581 gtgcgctgtt cgccctggcc gagcggcgcg gcgccgatct ggctgcgcgc acttacggcg
     2641 tggtcggtgc cggtcaagtc ggcagccgcc tggtggaggt gctgcgcggc ctgggctgga
     2701 aggtgctggt ctgcgatccg ccgcgccagg ctcgcgaggg cggcgacttc gtcggcctgg
     2761 aggagatcat ccgccgctgt gacgtgatca gcctgcacac cccgctgacc cgtgatggcg
     2821 acgatgcgac ctggcatctg ttcgacgttg cgcgcctggc gcagctcaag cccggctgct
     2881 ggctgatcaa cgccagtcgc ggcgcggtgg tcgacaacgc cgcgctgaag gcgcacctgc
     2941 agggcggtat cgatctcgat gtggtgctgg acgtgtggga gggcgagccg caggtggatg
     3001 tcgagctggc ggccctgtgc cgtctggcca ccccgcacat cgccggctac agcctggacg
     3061 gcaagctgcg cggcaccgag cagatctacc aggcgttctg cgcctggcgc ggcgagcaag
     3121 cacaagtcga gctggcccag ctgctaccac cggcctggct gggcgagctg gtgttgcatg
     3181 agcaggccga tccggcctgg gcgctggcca tggtctgtcg tgcggtatac gacccgcggc
     3241 tggacgacgc cgccttccgc cgcagtctgc tggcggacac ggagcagcgg cgcgccggct
     3301 tcgatgccct gcgcaagcac tatccgatgc gccgcgagct gcccgatctg acggtgcggg
     3361 taagcggggc ggcacaacaa ctgcgccgcc agttggcggc gctgggggta cggctggcct
     3421 gatcagccga acaggcggcg gaacggcttg gcgtcctgct ccagctgtcg gcaggcctgc
     3481 aggatcatct gttcggtgat cgggatttcc cgaccttggg cgtcgatgat cgcacccagc
     3541 tgcaggtggc gcagtttctg ggcggtatgc tggcggctgt gtgcgatgtg caggggggtg
     3601 tgcatgacgt gctctccttg acgttgacat cctccccgcc ctcaagcctt cggcttgcgg
     3661 ccataagcgg gaaggacggg gattcctacg gcgtcaggcg gcggtagctg catgaacgct
     3721 tcggtgggtt cctgctgctg acggcctgat tgcaccgttc acttcacagg cgatctgggc
     3781 atggtcctgc ccttctaact gcttcaatcc gcgctcacgg atgttcatcg cgccgaccac
     3841 atcggcgttt tcctcaaaac cgcaggcctg gcagaggaac tccgcctgac gctggcgatt
     3901 ttcggcatgg gtgtagccac aggccgtaca gtgctgactg gtgtactgcg gcggcacggc
     3961 ttccagccag ccgccacacc aggtggcctt gtagtcgagc tggcggcgga attctcccca
     4021 gccctggtcg aggatggatc tattgagccc cgacttctgc cggacgttcc gcccaggctg
     4081 ctcggcagtg ccgctggccg agcgggtcat gttgcctacc cgaaggtctt cgacacacac
     4141 cagcgcgtgg ttttggctga gggtgtgggt cgctttgtgc aggtagtcgt tacgggcgtt
     4201 agcgatctgg agctggatgc gctggacttt cgccttggcc ttgagccggt tacggctgaa
     4261 tttcgtcttg cgggccatct gccgctgggc tttggccagg cggtgctgat gagcggcgaa
     4321 gctgttgagc ggctccagga cggtttccct ctgtccgtcc cagagcgtgg cgaagcgcac
     4381 cacgcccagg tcgatgccaa cggcgagcgc cgaaggatgg cggggaatct cgacttcgcg
     4441 ctcggtctgg atcgatacgt accactgccc ggcacgcagg ctcacggtga ggttcttgag
     4501 ggtgccgagc acctcgcggc tattgcggta acgcatccag ccgagcttgg gcaggaagat
     4561 ccggctgttg gcttgatcga ccttgaattg cttggcatcc gggtagcgaa agctgtcgtg
     4621 ctttcctcgc ttcttgaagc gggggaagtc cgcccgcttg gcgaagaagt tctgatacgc
     4681 ccgatccaaa tcctttagcg actgttgtag cggatgcacc ggggcatcct tcaaccaggg
     4741 ggtttcatcg ctattgcgcc aggtggtgag ttgccggctc atggctgcgt agttgaggtg
     4801 cttaccaccg gcctcacggg tttccgtctg caacgccagg gccttgttga agacgaaccg
     4861 agccattccg gcgaactggc gcagcttgcg ctgctgttcg ccgttgggct cgatacgaaa
     4921 ctggtaggcc tggcgtttaa gcatgagcgt aatactgtcc atatgtacag gttcgagtat
     4981 agggcggctt cgcgccctgc gctatcctcc ccgccctgaa gtgcggggct tgtcgcgctt
     5041 tctggtcaat gagggcagtc tagaagcgcc agatgaagag cgggtgacag tttcccgagg
     5101 ccggaaagac aaatgcccgc aaccagtgcg ggcatttggc ggacggagcg cccggccggc
     5161 ggctgccggc cggcgcagcg tactcagcgg tattcgcaga ggtaggcggt atcctgggca
     5221 acctgcagct ggaatttgct gtcggccgga acgtcgaact tgctgccagc ggcgaaggtt
     5281 tcccaggcgt cgctgcccgg cagcttgacg gtcagggcac cggcgatcac gtgcatgacc
     5341 tcggccttgg cggtgccgaa ttcgtattcg ccgggcgcca tcacgccgac ggtggccggg
     5401 ccttcggtca tctcgaaacc gatggatttg actgtgccgt tgaagtactc gttgaccttg
     5461 aacatgcggg cctctgcata aaggagaaaa agggcgcgcc agtatgccca acgccggtgg
     5521 acacgtcacc cgtaccggca tcgattgcac ggtagaggca cgtcgtcggc gacggcctcg
     5581 gcgcaggccg ctagaatcgg tcgtcagatt cggtcaggaa caggcccatg gctatccgcg
     5641 cgttgctcgc caccgtgctg ctgttactcg gtggtgtgct gcaggcccag cctgtttcct
     5701 acagccgcga catccagccg atcctcacgc agaagtgcgt ggcttgccat gcctgctacg
     5761 acgcgccctg ccagctcaac ctgggcagcg gcgcgggcat ccagcgcggc gcctcgccgc
     5821 aggcggtgta caacggtaag cgtaccgagg cgcaggccac cacccgtttg ttcgtcgatg
     5881 cgcagggcga gcagggctgg cggacgcggg gcttccattc ggtgctgggc ggcggcgagg
     5941 cggcgctggt ggcgcgcatg ttggagctgg ggcgcagtca cgcgccgctg ccgggcagca
     6001 agctgccgga ggatctggac atcggcatcc gccgcgagaa ccagtgcccg cggcccgatc
     6061 agttcgacga cttcgcgcgc aagaatcccg gagctggcat gccctttgcc gtcaccgggc
     6121 tgagcgatgg cgagtacgcc accttgcggc gctggctggt ccaaggcgcg ccgctggatg
     6181 gcgaaccgct cgagccgaat gcctcggagt cggcgcagat tgccgagtgg gaggcgctgt
     6241 tcaaccgccc cggtgcccgc gagcagctgg tggcgcgctg gctgtacgag cacctgttcc
     6301 ttgcccacct gtacttcgac gcgggcaaga gcgggcactt cttcgagctg gtgcgttcgc
     6361 gcacgcctcc cggcgagctg gtggacgtga tcgccacgcg ccgccccaac gacgacccgg
     6421 ggacgcactt cttctaccgc ctgcggccga tccagggcgt gatcgtgcac aagacgcata
     6481 tcacctatgc cttgaacccg cgcaagcagg cgcgcatcga gacgctgttc ttcgccgctg
     6541 actggcaggt ggacgcggtg cccggctacg gcccgcagcg ccgcgccaac ccgttcgcga
     6601 ccttcgccgc catcccggcg caggcacgct accagttcat gctggacgac gccgagtact
     6661 ttgtgcgcac ctttatccgt ggcccggtgt gccgcggcca gatcgccacc gacgtgatcc
     6721 gcgaccactt ctggacgctg ttccaggccc cggcccacga cctctacctg actgatgcgg
     6781 cctaccgcga gcgagtcacg ccgctgctgg cgctgcccgg cgtgcaggac ggcctggctc
     6841 agctaccgct gctctggcac gagtccaagg ccaatcgcaa tgcctacgag gcggcgcgcc
     6901 gcgaggccta ctcgcacacg ccctacccga gctggtcgca tatctgggcg ggtaacgaca
     6961 atgcgctgtt gagcatcttc cgccagctgg acagcgcctc ggtgcgcaag ggcctgatcg
     7021 gcgcggtgcc gcagactttg tggtggctgg acttcccgtt gctggagcgc acctattacc
     7081 agctggtggt caacttcgac gtattcggca acgtctccca ccaggcgcag acccggctgt
     7141 acttcgacct gatccgcaac ggtgccgaac agaatttcct gcgcctgatg cccgccaatc
     7201 aacgccaggc gatcctcggc gattggtacc aggacggcgg caagctcaag ctgtggctgg
     7261 actacgaggg catcgacgct gacacgccca gcggtctgcg cctgcctgca gacgatccca
     7321 aggcggcgtt cgccgagcgc ctgctgcagc gctacggcag cctcaatgcg cggcccgacc
     7381 cgctcaaccg ttgccgcgac gagcgctgtt tccgcccggg ggcgagcccg gcgttgcagc
     7441 gcatggacca gcgcctcagc gtcctggcgg cgcgcccgct gagccagctg ccggccatcg
     7501 aatacctgcc ggaagcgacc ctgctgcgcg tcgagctgga tggcggcggg cgcgaggtct
     7561 acagcctgct gcgcaaccgc gcgcacagca acgtggcctt cctgctcggc gaatcgctgc
     7621 gctaccagcc ggagaaggac accctgacgc tctatccgga agtgctgagc agctacccca
     7681 acttcctgtt cagcctgcgc gagggcgagg tgaatgcctt cgtcgctgcg ctgcagcagg
     7741 cacgcagcgc ggccgacttc gagatgatcg tgcgccgctg gggcgtgcgc cgcacccatc
     7801 cgcagttctg ggcgttgttc catgacctcg gcgagcacat tcgcgagcac gagccggtgg
     7861 aggcgggggt gctggacatg aaccgctatc agaatctgta ggtctcaccc gcctgtggga
     7921 gcgcgcaccg accggtctgc aggttattcc tactaaaatg ctgtgacttt atccgcgccc
     7981 cggcatggcg tacactgcgc ccatatttgc gaggagtcgc catgagcaac atcaccatca
     8041 ccgaatcggc tcaggaatac ctggccgacc tgcttcaaaa gcagaacact ccgggtatcg
     8101 gcattcgcgt gttcatcacc cagccgggca cccagtacgc cgaaacctgc atcgcctact
     8161 gcaagccgag cgagcagaag gccgaggata ccccgctggc gctgaaggac ttcaccgcct
     8221 ggctggatgc ggtcagcgtg cccttcctcg aagacgccgt ggtcgactac gccaccgatc
     8281 gcatgggcgg ccagctgacc atcaaggcgc cgaacgccaa ggtgccgatg gtcaacgagg
     8341 acagtccgct gcccgagcgc atcaactact acctgcagac cgagatcaac cccggcttgg
     8401 ccagtcacgg cggcatggtt tcgctggtcg acatcgacga cgaaggcgtc gccatcctgc
     8461 gtttcggcgg cggctgccag ggctgcggca tggtcgacat gaccctcaag gacggtgtcg
     8521 agaagaccct gatggagcgc atccccgaac tcaagggcgt gcgcgacgtg accgatcaca
     8581 gcaacaagga aaacgcgttc tactgagttt cgcgatccgg tctttcataa agggcgggaa
     8641 gccgcccttt tcgtatcgta ttccgtaatt gcagttatca ggaaagcatc taagtccccg
     8701 ccatgctgtt tgctagtttc attcatgcaa cgccaatgcc ccttcgggtt gcattagcga
     8761 ataaccagga tggaacatgc acaagtcaat gttgcgtcga ctgcttgttg ttgacctttt
     8821 cgatgaattc aatcccatcg ctcccgaagt acggagcgcc ggctggatag tcgatctctg
     8881 ctccctggac gaggcatttg atcgtcaggc cgatgtcggc ctgatacgcc tggggccttg
     8941 tctggactcg gggggcaaac tgttgcggcg cacgccgatg caatggatcg ctgccctccc
     9001 gccagatatc tcgctggacg gcagccttgg cctgttcgtt ggcgaatggt gcttcgactt
     9061 ccacaccttg ccattcgacc ggccgcggct gctggtcacc ctgggacacg ctctaggcat
     9121 ggctgcgctg cgccgggccg agcaggtcga cggtggtgac gagctgctcg gcgatgcgcc
     9181 gtgcatgcgc gagctgcgca agctgctcgg caagctcggg ccgaccgact cgccggtgct
     9241 gatccgcggc gagagcggga ccggcaagga gctggtggcc cacgctctgc accgcttgtc
     9301 gcgtcgcgcc gagcaggcct tcgtggcgat caactgcgga gctattcccg agcaactgat
     9361 ccagtccgaa ttgttcggcc atgagaaggg cgccttcacc ggcgcccacc agcgcaagat
     9421 cggccggatc gaatcggccc atggcggcac cctgttcctc gacgaggttg gcgacctgcc
     9481 gctggatctg caggccaacc tgttgcgctt cctgcaggag gggcagatcg agcgggtcgg
     9541 tggcaaccag ccgatcgagg tcaatgtgcg ggtgctggcg gccacccaca tcgatctcga
     9601 gcaggcggtg gagcgtgggc gtttccgtga ggatctctat taccggctga atgtcctgca
     9661 ggtgcatacc agccccttgc gtgagcggct ggaggacgtg ccagctctcg cccgacactt
     9721 tgcccggctg tacgccgacg aggtcgggcg gcggccgcgc tcgttcagcg aggaggcgct
     9781 gcgggcaatg ggcgaatatg cctggccggg caacgtgcgc gagctggcca accgggtgcg
     9841 ccgcggcctg gtgctggccg agggacagca gatcatggcg caggacctag ggtttcgtgg
     9901 ggtttgcgta ccgacgagct tggattacag ccttgagaac tacaagatgc aggccgagaa
     9961 acaggcgctg agcgatgtgc tgcgccgcta ctcggcgaac ctcagtcacg cggcccgggc
    10021 gctgggtgtg tcgcggccga ccttctaccg cctgctgaac aagcaccaga ttcgttgagc
    10081 gcgagatagc aaaggcccct gccggattcc tcccggcagg ggccttttgc gtgatgagac
    10141 tgccgcgctg gattcagaag tagtagggga atttcaggct gaaactgaag tccggtgagt
    10201 ccggggtcag gcctatcgac aggttgggca ccatggtcag cttgtcgctg agggcatagg
    10261 tcatgccgat attgaagtag gcggcattgg catcgctgct ggaaaccgtc tgccagctgc
    10321 cgccatccgg cttgatcttg ctcttctggc tgatcagttg cgagtaggac agcgacaggc
    10381 tcatgcgctc gttgagggcg aaggccgtac ccaggccgta ctggaaccag ttgcccatct
    10441 tgaccttgcc acccaccttc tggttctgga ccgggctgat gtcgtcgaag gactcctcga
    10501 ggttgtaggt ataggacagg ttgccgaaca gcacggccgg gtccaccgtc ttgaccaccg
    10561 agatgcctgg cgtaaccgac cacacgccgt tgccggtggg caggtcctcc ggaaccgaca
    10621 ggttgtcgtt gtccggcgac tgcaccagct tgataccgta ggggtcttcg ccggttggcg
    10681 ccttcacccg caggctgaat accgtatctg gccggctttc gctctcgtcg agaaacttgt
    10741 aggccacccc gaagctgaca tcgccgagtc gggggtcgcc ggtgacgctt tccgacgaaa
    10801 cctgagtggt cgaaccgcca gcgccagccg actcgtaggt ggactcccga tagaccagcg
    10861 gcacgttgag atcgaactgc caacgattgc ccaggttgta gcgaccggtc atgtccaggg
    10921 tgaagttatc ggaattgatc tggtcgacgt tgatgttgcc gagaaagatg gcgtccagca
    10981 aaagcaggcc gttgaggccc agctggcggg tatcgtagtg gctgtatgtc aggccggttt
    11041 ccaggctgaa cgtgccttcg ccgaagaagc cgctggcttt gtcgtagatg ttctcgacac
    11101 tgcgcgccgg ggtgttgtct tcgcgcaggc tttcgccgta gccggcagcg ggttggctgg
    11161 tggaggaggg cgagcgggcc agtcgctgtg gctggggaac ggcaggttgt gtctcgacct
    11221 cgcgtacccg ctgttccagc accatgaggg ctttctgctg ggcgtcgtaa cgctgacgca
    11281 attcgagcag ggcctgctgc agttctctta gctcggcatc ggaggccgca tagaggagtg
    11341 tcgaaggcag tagcgcggca atgcccactg ccacgcgaag cgagtgtgac tgaagcatgg
    11401 gaagtcccta tcgcgattat ttgtcagtgt atccagcgta gatcagtaac ccgcagggcg
    11461 cagggcgcgc agttgatcca ggttgacccc catcgaactt gccgggattg gcgtgtcgcg
    11521 cagtaccaca tccagctcgg tcaggttcct gaccatattg ctcgatccgc cgatgttggc
    11581 atgctgttgc agtccgccac ccgccagttg ttgcaggctg gtgccttggc caagagcgtt
    11641 gatggcgatc ctgaagccgc cgtcgctggc gctgatctgc acattgccgg cctgggtgct
    11701 ctcgatctgg ttggcaaatg gctggccggc attgcccgca ttcggagctg aaccgccgcg
    11761 tttgacgttg acgacgaaat tgttcgctgc gctattgaag tcgccggccg agcgtacgct
    11821 ttgggtcacg ccgttgacgg tgctcaggcc ctcaccgcca tagatttgtc cgctgcccgc
    11881 cgccggggcg ctctggtcgc cgttttgggt gatggtcgaa acgttgaact gcggctggaa
    11941 catcccttcg ctcgcctgca tgtggaccgc gccggcgagc acctggccgc tggcgttctc
    12001 ccacacgctg ctcatggtga cgccaaagtg gacgatgtgc ccgggcatga cgaaacggcc
    12061 gcgcaattgg gaaagctcct ggtcgctcag ttcgaccggc tgcagcgggt tgctggcctg
    12121 agcgcaaatg ctggcggcaa tgcaactggc agccagtaat acatgaatct tcatgtcgac
    12181 ctccggcgga gacttcctgc ccgtgatgtc agaatgattt gtctgtgatc aaaagaaatc
    12241 gctctggatg aatccatgtt ccatcagttc ggcatccttg acgggctgga atgcattcag
    12301 gcgatctttg gctgacaacg gcttgggcgg attcaatagg gcattggttt tgtcgtactc
    12361 tcggccaatc accgcgaaga caattccatt ccaacctttg aggaaatcct caaccttgta
    12421 gcgtttatgt ccgaaaatcg ggtcgccaat ataggcccag ccattttctg ttctttgcaa
    12481 taccacgaag tgtttgtagc cgcggacttc ctgcagcacg atagccggaa ccttgagttg
    12541 ctcgaggccg gcagcaggaa cgcggtagcc gcgggcgcgc atgccgaggt tctcgacata
    12601 gcgcttcatg tcgagcatgg aaaagccctg ggtacgcacc agctcatggt cggcaccggc
    12661 cagcatgccg ttgatcacga acgtctcgtc cacatcgaat cgataggctt cgcgcaggat
    12721 ggttgccaag gcggcggcac cacagctgaa gtcggttttt tgttccacca gattgctgaa
    12781 gcgccgttca cggatgcttt cgacgtgctt gaacgcgatg gcaccgccgg gcagtacggc
    12841 gaaagggact tgtgcggctt gcagcgcagt actcagacaa cacaggacag gcaggatggc
    12901 cagacgcatg gggcacactc cctatggcta taagggaagc cctgcgggtg cagggctccc
    12961 ttcgcgtgtg gttgcttagt tgccgttgtt gcaggaattg cagccgacgg cgacagccag
    13021 ggtgttcagc tgctggttgc tgctgcccga ggcaatgttg acgccgatgt tgccgctggc
    13081 gttattgagc gagtcgctga gggtggcgct gttgatgacg ggattggcga aggtgtagct
    13141 cttcgtgaag gtgacggtgg cagccaaggt gctgttgccg gcctcttcga tgaggctgtt
    13201 ggcgctatgg ctttcgttct tctcagcact ggccgaggcg ctggcctcgc tgctcttctc
    13261 gaaggaggcc tcgaagctct tgtccacgct acgagaatcg tcagccgtgt aggaagagct
    13321 cgcgtccacg ctcttggcga ggtcgaaatg cgctgcggac tcctcgctcg cattttcggc
    13381 cgcgttggca ttggcatcga tttcgagggc cccatcgcca tgggcatgaa tccttccggg
    13441 cgaaacaacc agatggccgt caaggctggc gctggcggtg gcgtctagat gggccgtctt
    13501 gtcccactcg ctggaggagc tgctcgactt gttcgcgtcc acgacgacat ccagactggc
    13561 gttgtaggtg cggtcggagc tctcggacga gtcatggctg gccgagagcg aggccgaagc
    13621 ggtcgctgac ttagcactct gcgcctgcca cgaaaaatcc caggagtcgc tcgaatcact
    13681 ggattccgcc ttcgagtagt tgttgctcca gtccttggtg acatcgaagg ttgtctcgac
    13741 ggtcacgctg gcggccttgt tgtcgacgcc gccgcgcagg tcctgattgc cggtggcagt
    13801 ggcagtcgca tcccagcccc tggccgtggc gatcgccagg gcgttctgct gctggttgag
    13861 gttgccgctg gccgcgttca cgccgatgtt gccggaggca tcgttgccgg aaccgttcac
    13921 cgtcgctgcg ttgctgccgc cgaggttgta ggtccagccg tcgttgtcct gatcgatggt
    13981 ggagtcggct tcggcgctgc caaagacgaa ggcctcatcg gcggtggcaa tcgcgacatt
    14041 gttggcttgc tggttcatgc tgccagcggc aacgttggcg ccgatgttgc cgctgctgct
    14101 cagcgagttc tcgatgttga cgctgttttc ggtgccctca ttggtgacct gggcgtcgcc
    14161 gatgtattgg tcgcggcctg cgtaggcagt ggcatcgcca gcgttgcggt ccagcagggg
    14221 gtcgtgatcg tggttgtgac catttgattg gtttgcttgg gccgccatcg ccatcgcggc
    14281 agccatggcg aagaccagcg gtttcagagc cattgacact ttcatggtag ttctccttgc
    14341 agttttttat gcaacacgtg ttggtcttgc gttggataac gagtcaatcc atgaccctga
    14401 tgctgacggt gttggccatc cggtttccca ccccagcgct ctggttcagc tgaactacac
    14461 cactgctgcc ggcgaaggcc tgtctgtcga tagcgacggc acgctctccc ggctttgtct
    14521 cagccgtacc tgagttctgc gcaagcacca cgttctgctg ggcgaggatg ctgtcatcca
    14581 cgctttgtgc cgcagaaccg acgctcatgc gaaaagagtt gatctgttga gtgccggcgc
    14641 cagccgactg attgacgccc aataccccgg aaccattgct gaaggcgcta ccggtgatgt
    14701 tggcctgggc attcatcgag gtgtcgacgc cgtccagctc ctggtactga cgaaagttgg
    14761 tggtggcatc agcgccaatc gccagtgcgc ggccgttgat ctgttgctgt tggctgccgg
    14821 cggcctgatt gaccgacagc acgccttggt aggttctgcc gctgttgtcg attgttgcgt
    14881 ggttgtttac ctgcgcttgt tcggcgaggc tggcgacggg tatggtcatg gcgataagca
    14941 gggtgagtcg cagcatgtca gcgatctccg gtgaggattt tcagcggtgc caacccctgc
    15001 tgaaggctcc gattgatctg gttggagacg ttgctgccag ctccgccact ctggccggcg
    15061 cgcatgccgg gcaggccatg cgaagaggct tgggatgagt tactcaagcc tggcagttgg
    15121 ccgctgggaa gcatctggcg ttgcaggctg gatccgctgg tgaccctggc aaagtcggca
    15181 tcagtcaact cgaacgaatt ggtcgctctg atgacggtgc tggaggggtt ggcattgacg
    15241 gtgcgcggat tgggatcggg gagcagggcg gggcgggtgg caacccgcgg ctgcacgtcg
    15301 cgggtgatga cgatgatccc gtcggcggag tgggcaggga tggcagctcc catggctgag
    15361 agcatcagca tggccagcag tgccggacga gaaatccttg aagcgtttcg atccatggca
    15421 gcggttccta cgcgattcag acccttttgc gtgcaagggc atacgcatgt gccgtgccag
    15481 tttctaaatc ttcaattgaa tcagtgggtt ggggttttcc ttgaatagcc gggaaaatcc
    15541 gctgtcaacg cgttgaaaca gtggggtgtg tgtctgcgct gttgggccgg ctaatccgcg
    15601 gcctgcatgg cgattgaggg tgttgcaggc ttgatacact ccgccgggtc aggaccgcaa
    15661 agcccgtcag ccgcggtatt tgccggcaac ggtctgtatc atccgcgcaa cacccccgcc
    15721 gttactgcat cactgcaggt cgccactcgc acaggtcaag gagagaacat gcatccgttt
    15781 accgcccccc gtccgcccca tctggcctgg ctgctcggcc tggccgggct gattccgttt
    15841 atcagtggcg cactggggat ctggatcacg ccggtcggct ggcgcccgct ggtactgact
    15901 gcgctcctcg actacgccgc ggtgatcctg gcgttcatgg gggcgattca ctggggactg
    15961 gcgatgcgtg ccgaacagga cgacctgcgt gcccagctgc agttgggtct gtcggtgatt
    16021 ccgccgttgc tgggctgggc agcggtatcc ttcgggttgc cggcggctct ggccattccg
    16081 gtattcgtgc tggctttcgt cggcctgtac atggccgacc tgcgcgccgt gcgcctgggg
    16141 ctggcaccgc tctggtaccg ccccctgcgc atgccactga ccgtggtggt ggtcgcctgc
    16201 ctgctgattg cctgggccgg ggtactggcg ggctgactca gcagctcagc tggtcggcct
    16261 ggcggtagag catcagcagc ttgcgggtca cggtctgctg caggtcgttg cgctcgaagg
    16321 cggacagatg tggcagctgg gtcagctgca ggcggtgcag gtgaaggtag ggcatgcgct
    16381 ggtcgaattc gcgcaggtcg cccttcatca gtaccggcag gaacacgtcg ccattctgtt
    16441 tcagcttgct gatgatctgc gccagggcgc tcttgaacgg gcgcgcctga agcatctcgc
    16501 cctgcgcgcc cagacgggcc agcagcagtc cattgtgccc ctggaactgc cccggcagta
    16561 cgcacaggcc ggtcttgcgt acggaatagg gcgaaatgtg gttcagggtg tcgtggaagg
    16621 cgtaggggtt gggcagcacg accatcttgc ggcccagttc ggcggggaag tgcaggccgt
    16681 agttggtata cagctggctc accgactcgg agtagacgca cagacggttc tcgaacaggc
    16741 gaatgaagcg ctgtgccagc tcgcgctgct ccaggcgatc caggtcccag atcaggtcca
    16801 ggttcggttt cttgtccagg ttcacttcct ggaagttgat cgtgctcatc gcgacggcgc
    16861 catgcatgca ggtggggtcg ttgccgctgg cggcgaacag ccagggggta atcgtcagcc
    16921 cctccgtggg caggcgacaa caggtggtgg cagagtatgc gcaaagagtg tgacaaccca
    16981 ccagttgtct tgcgcagatt tgcgatcaaa gtcggccatc cgccactgcg gacgcaagcc
    17041 gaatgatccg gactgaccct ggtcaacgcg ctatgggcca agacctgctg ccggccggcc
    17101 agctctggta ggatgccgcc cggttatcca accgacgccc tgctgcgcgc cggtcggcac
    17161 ttatcaatca tgagcaagga cgcgcgacag gcgcgtgacg gggagttttt gcatggaatt
    17221 ctggaccgcc attcaggcgc tgatcctggg tatcgtcgag ggactgactg agttcctgcc
    17281 gatctccagc accggtcacc agattatcgt cgccgacctg atcggcttcg gtggcgaccg
    17341 cgccaaggcg ttcaacatca tcatccagct cggtgccatc ctcgcggtgg tgtgggagtt
    17401 ccgacagcgc atcttcggcg tgatttgcgg cctgccgcgc gagcgtcagg cgcagcgctt
    17461 caccgccaac ctgctgatcg ccttcttccc ggcggtgatc ctcggtctgg ccttcgccga
    17521 cctgatcgag cactggctgt tcaatccgat caccgtggcc agcgcgctgg tgatcggtgg
    17581 cgtggtcatg ctctgggccg agaagcgcca gcacgccatt gaggtggata cggtggacga
    17641 catgagctgg aagcatgcgc tgaagatcgg ctgcgcccag tgtctggcca tgatccccgg
    17701 cacctcgcgc tcggcctcga ccatcatcgg cggcctgatc ttcggcctgt cgcgcaaggc
    17761 ggcgaccgaa ttctccttct tcctcgccat gccgaccatg gtcggtgccg cggtctactc
    17821 cggctacaag catcgcgcac tgttcgagaa cggcgccgat ctgccggtat tcgccctcgg
    17881 cttcgtcacc tcgttcgtct tcgccatgct cgccgtgcgc gccctgctga agttcatcgc
    17941 cagccacagc tacgcagtgt tcgcctggta ccgcatcggc ttcggcctgc tgatcctcgc
    18001 cacctggcag ctgggcatga tcgactggag cacggcgcag ggctga
//
LOCUS       DATCUN010000126         5660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_118077, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000126 DATCUN010000000
VERSION     DATCUN010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118077"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..1855
                     /locus_tag="VIO83_05025"
     CDS             344..1855
                     /locus_tag="VIO83_05025"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY8330674.1"
                     /translation="MTTHSAIPTVKLLINGQFVESTTSQWRDVVNPATQEVLARVPFA
                     TTEEMNAAVASAKEAFKTWRKTPIGVRSRIFLKLQQLIRDNIKELAAILTAEQGKTLP
                     DAEGDVFRGLEVVEHASGIGNLQLGELANNVAGGVDTYTLLQPLGVCAGITPFNFPAM
                     IPLWMFPMAIASGNTFVLKPSEQDPMVTMRLAELALEAGIPPGVLNVIHGGVEAVNLI
                     CDHPDIKAVSFVGSTKVGTHVYNRATQNGKRAQCMMGAKNHAIILPDAHKQQTLNNLV
                     GAAFGAAGQRCMALPVVILVGEAQGWLPELVEKAKSLKINAGCEPGTDIGPAVSCNAL
                     DRISGLIEQGVREGAQLVLDGRNPQVPGYPNGNFVGPTIFSGVSTEMSIYKEEIFGPV
                     LCVLQAATLDEAIALINANPNGNGTAIFTRSGAAARHFQEEIDVGQVGINVPIPVPVP
                     IFSFSGSRASKLGDLGPYGKQAVMFYTQTKTVTQRWFDEDEVGGPVNTTITLK"
     gene            1907..2680
                     /locus_tag="VIO83_05030"
     CDS             1907..2680
                     /locus_tag="VIO83_05030"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003300487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY8330675.1"
                     /translation="MEFETLLLEVQGRVGLITLNRPQALNALNSQLIAELNLALDHFE
                     KDPQISCMVLTGSAKAFAAGADIKEMADLTFPQIYLDDFFAPADRIANRRKPLIAAVA
                     GYALGGGCELALMCDFIYAADNARFGQPEINLGVLPAIGGTQRLTRALGKAKAMEMCL
                     TGRQMDAHEAERAGLVARVVPVESLLEETLKAAHAIAEKSLPSTMMVKESINRVFETS
                     LSEGVRFERRVFHAVFATADQKEGMAAFVEKRKPAFKNR"
     gene            2716..3612
                     /gene="mmsB"
                     /locus_tag="VIO83_05035"
     CDS             2716..3612
                     /gene="mmsB"
                     /locus_tag="VIO83_05035"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEY8330676.1"
                     /translation="MHIGFLGLGNMGAPMARNLLKAGYQLTVFDLSAAAVSSLVEAGA
                     KSAASPAALAQADVELIITMLPAAAHVKSVYLGEDGLLAHVRPGVTLIDSSTIDPLSA
                     REVASAARQHGNPMLDAPVSGGTGGAMAGTLTFMVGGDAADFDRAQPILAAMGKNIVL
                     CGGSGNGQAAKVANNMLLGISMIGVAEAMSLGVALGVDPKVLAGIINTSSGRCWSSDT
                     YNPFPGVLENVPAARGYSGGFGSDLMLKDLGLASEAAKQVRQPVILGALAQQLYQSFS
                     LQGHGGLDFSAIINLYRKDTEA"
     gene            3609..4688
                     /locus_tag="VIO83_05040"
     CDS             3609..4688
                     /locus_tag="VIO83_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY8330677.1"
                     /translation="MSEAPVLATVRNRIGHLTLNRPAGLNALTLPMVRILHRQLQAWA
                     DDPEILAVVLRGTGEKAFCAGGDIRSLHDSYKNGDTLHSQFFEEEYALDQYIHTYAKP
                     ILALLDGFVLGGGMGLIQGASLRVITERVKMGMPETAIGFFPDVGASYFLSRLPGELG
                     VYLGVTGVHIRAADALYARLADWCLPSEQVAELDRCLDSMSWNVHPKEALRTLLATLA
                     TNKLPGSELKALQPAIDEHFAQADIAAIRNALLAETRPEFHDWAEETAKLLYSRSPLA
                     MSVTLEQLRRGRSLPLQECFRMEHLLGSQWFAKGDIMEGVRALIIDKDKSPRWNPDRI
                     DAVTPLQVDEFFIDFKPANKSRRAG"
     gene            4738..>5660
                     /locus_tag="VIO83_05045"
     CDS             4738..>5660
                     /locus_tag="VIO83_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019649662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8330678.1"
                     /translation="MHDIELTEEQRMIRDMARDFARGEIAPHAQAWEKAGWIDDALVR
                     QMGELGLLGMVVPEEWGGSYIDYVAYALAVEEVSAADGATGALMSIHNSVGCGPILKY
                     GTQAQKEAWLPDLAAGRAIGCFCLTEPQAGSEAHNLRTRAELRDGQWVINGAKQFVSN
                     GKRAKLAIVFAVTDPELGKKGLSAFLVPTATPGFVVDRTEHKMGIRASDTCAVTLDNC
                     RIPAENLLGERGKGLAIALSNLEGGRIGIAAQALGIARAAFEAALAYARDRVQFGKPI
                     IEHQSVANMLADMHTRLNAARLMVLHAARLR"
BASE COUNT         1060 a         1972 c         1706 g          922 t
ORIGIN      
        1 gatgcatcct cgcttttctt atttttgtca tcagcttgat ggaaaccttg cacgccctgc
       61 ggtcacgtta aaaaccgaca ggaaggtttt tatttaagct atccgctccc ctcctcctgc
      121 gcaagccttg gtggaaaaaa atcttccgtc tgcatcctcc tgcgcaaatg aaccgttggt
      181 aacggctgcc tgcgcttggc cgcacgagac tgtgcaaatt tgcagataca gcctgcattt
      241 accgccattg caccagtcaa atttacactg ctagtctcaa ttcaaccctc agcgcagcgc
      301 tgggaacaca acaataacga gagcctgaac aggcggggta tccatgacaa cgcacagcgc
      361 catccctacc gtaaaattgc tgatcaacgg ccagttcgtc gaatccacca cttcccagtg
      421 gcgcgatgtg gtcaacccgg ctacccagga agtcctggcc cgcgtgccct tcgccaccac
      481 cgaggagatg aacgccgcgg tcgccagcgc caaggaagcc ttcaagacct ggcgcaagac
      541 cccgatcggc gtgcgctcgc ggatcttcct caagctgcag cagctgattc gcgacaacat
      601 caaggaactg gcggctatac tcaccgccga gcagggcaag accctgccgg acgccgaagg
      661 cgacgtgttc cgcggtctgg aagtggtcga gcatgcgtcc ggcatcggca atctgcagct
      721 cggcgagctg gccaacaacg tggccggcgg cgtcgacacc tacaccctgc tgcagccgct
      781 gggcgtgtgc gccggcatta ccccgttcaa cttcccggcg atgatcccgc tgtggatgtt
      841 cccgatggcc atcgccagcg gcaacacctt cgtcctcaag ccctccgagc aggacccgat
      901 ggtgaccatg cgcctggccg agctggcgct ggaggccggc attccgccgg gcgtgctcaa
      961 cgtcatccat ggcggcgtcg aggcggtcaa cctgatctgc gatcacccgg acatcaaggc
     1021 ggtgtccttc gtcggttcga ccaaggtcgg cacccacgtc tacaaccgcg ccacccagaa
     1081 cggcaagcgc gcgcagtgca tgatgggcgc gaagaaccac gccatcatcc tgccggacgc
     1141 ccacaagcag cagaccctca acaacctggt cggcgccgcc ttcggtgcag ccggtcagcg
     1201 ctgcatggcg ctgccggtgg tgatcctggt gggcgaggcg cagggctggc tgccggagct
     1261 ggtcgagaag gccaagagcc tgaagatcaa cgccggttgc gaacccggca ccgatatcgg
     1321 cccggccgtg tcgtgcaacg ccctggaccg catcagcggc ctgatcgagc agggcgtgcg
     1381 cgaaggcgca cagctggtgc tggatggccg caatccgcag gtgcccggct atccgaacgg
     1441 caacttcgtc ggcccgacca tcttctccgg cgtcagcacc gagatgagca tctacaagga
     1501 agagatcttc ggcccggtgc tctgcgtgct gcaggccgcg accctggacg aggccatcgc
     1561 cctgatcaac gccaacccga acggcaacgg caccgccatc ttcacccgct ccggcgccgc
     1621 cgcccgccac ttccaggaag aaatcgacgt cggtcaggtc ggcatcaacg tcccgatccc
     1681 ggtgccggtg ccgatcttct ccttctccgg ctcgcgcgct tccaagctcg gcgatctggg
     1741 cccgtacggc aagcaggccg tgatgttcta cacccagact aagaccgtca cccagcgctg
     1801 gttcgacgag gacgaggtcg gtggcccggt caataccacc atcaccctca agtaactcgc
     1861 caaggccggt gccgctgggc accggcctgt acctggagaa gtcgatatgg aatttgaaac
     1921 cctgctactt gaggtgcagg ggcgcgtcgg tctgatcacc ctgaaccgtc cccaagcgct
     1981 caacgccctg aacagtcagc tgatcgccga actgaatctg gcgctggatc atttcgagaa
     2041 agacccgcag atcagctgca tggtcctgac cggctcggcc aaggccttcg ccgccggtgc
     2101 ggacatcaag gaaatggccg acctgacctt tccgcagatc tatctggatg acttttttgc
     2161 ccctgccgac cgcatcgcca accgacgcaa gccgctgatc gccgcggtag ccggctatgc
     2221 cctggggggc ggctgtgaac tggccctgat gtgcgacttc atctacgccg ccgacaacgc
     2281 gcgcttcggc caaccggaaa tcaatctggg cgtgttaccc gccattggcg gcacccaacg
     2341 cctgacccgc gccctgggca aggccaaggc catggaaatg tgcctgaccg ggcgccagat
     2401 ggacgcccat gaagccgagc gcgccggact cgttgcgcgg gtggtgccgg tcgagagcct
     2461 gctggaggaa accctcaagg cagcccatgc catcgcggaa aaatcgttgc cgagcaccat
     2521 gatggtcaag gaaagcatca accgcgtctt tgaaaccagt ctgagcgaag gcgtacgttt
     2581 cgagcgccgg gtgttccacg ccgtgttcgc cacggccgac cagaaggaag gcatggccgc
     2641 cttcgtcgag aagcgcaaac ccgccttcaa gaaccgctga gcgcagcacg cccataacaa
     2701 caagaggaca ccaccatgca tatcggcttt ctcggcctcg gcaacatggg cgcgcccatg
     2761 gcccgcaacc tgctcaaggc aggttaccaa ctgaccgtat tcgatctgtc cgccgccgcc
     2821 gtcagctccc tcgtcgaggc cggcgccaag tccgccgcgt ccccggccgc cctggcgcag
     2881 gccgatgtcg agctgatcat caccatgctg ccggccgccg cccacgtgaa gagcgtctat
     2941 ctcggcgagg acggcctgct cgcccatgtc cgtcctggcg tcaccctgat cgactcctcg
     3001 accatcgacc ccctgagcgc ccgcgaggtc gcctccgccg cacgccagca cggcaacccc
     3061 atgcttgacg cgccggtctc cggcggcacc ggcggcgcta tggccggcac cctgaccttc
     3121 atggtcggtg gcgacgcggc cgatttcgac cgcgcccagc cgatcctcgc cgccatgggc
     3181 aagaacatcg tgctctgcgg cggttcgggg aacggccagg cggccaaggt ggccaacaac
     3241 atgctgctgg gcatttccat gatcggcgtc gccgaggcga tgagcctggg cgtggccctg
     3301 ggggtcgatc ccaaggtgct cgccgggatc atcaacacct ccagcgggcg ctgctggagt
     3361 tccgatacct acaacccatt ccccggcgtg ctggagaacg tccccgcggc acgcggctat
     3421 agtggcggtt tcggttccga cctgatgctc aaggacctgg gcctggccag cgaagcggcc
     3481 aagcaggtcc gtcagccggt gatcctcggc gccctggccc agcagctgta ccagagcttc
     3541 agcctgcagg gccatggcgg cctggatttc tccgccatca tcaacctcta ccgcaaggac
     3601 accgaggcat gagcgaggct cccgtactgg ccacggtgcg caaccgcatc ggccacctga
     3661 ccctcaaccg cccggccggc ctgaacgccc tgactctgcc gatggtgcgc atcctgcacc
     3721 ggcagctgca ggcctgggcg gacgacccgg agatcctcgc cgtagtgctg cgcggcacgg
     3781 gcgagaaggc cttctgtgcc ggcggcgaca tccgctcgct gcacgacagc tacaagaacg
     3841 gcgacaccct gcacagccag ttcttcgagg aggaatacgc cctcgaccag tacatccata
     3901 cctacgccaa gcccatcctg gcgctgcttg acggcttcgt cctcggcggc ggcatgggac
     3961 tgatccaagg cgcctcgctg cgggtgatca ccgagcgggt gaagatgggc atgccggaaa
     4021 ccgccatcgg cttcttcccc gatgtggggg ccagctactt cctctcgcgc ctgccgggcg
     4081 aactgggcgt ctacctcggc gtcaccggcg tacatattcg cgccgccgat gcgctgtatg
     4141 cccgcctggc cgactggtgc ctgcccagcg agcaggttgc cgagctggat cgctgcctgg
     4201 acagcatgag ctggaacgtg caccccaagg aggcgctgcg taccctcctc gccaccctgg
     4261 ccaccaacaa gctgccgggc tctgaactca aggccctgca gccagccatc gacgagcatt
     4321 tcgcccaagc ggacatcgcg gccatccgca acgcgctgct ggccgagacc cgcccggagt
     4381 tccacgactg ggcggaggaa accgccaagt tgctgtacag ccgttccccc ctcgccatga
     4441 gcgtcaccct cgagcaactg cgccggggcc gcagcctccc cctgcaggaa tgcttcagga
     4501 tggagcacct gctgggtagc cagtggtttg ccaaaggcga catcatggaa ggcgtgcgcg
     4561 cactgatcat cgacaaggac aaatcgcccc gctggaatcc ggaccgcatc gatgcggtca
     4621 ccccgctgca ggtcgacgag ttcttcatcg atttcaagcc cgccaacaag tcccggcgcg
     4681 ccggctgaag atccgccggg acacgccaat cagcttaaat aagaagagag accgctgatg
     4741 cacgacatcg agctgaccga agaacaacgc atgatccgcg acatggcccg cgacttcgca
     4801 cgcggcgaga tcgctcccca cgcccaggcg tgggaaaagg ccggctggat cgatgatgcc
     4861 ctggtccggc agatgggcga gcttggcctg ctcggcatgg tggtaccgga ggaatggggc
     4921 ggcagctaca tcgactacgt cgcctatgcc ctggcggtgg aggaagtgtc tgccgccgac
     4981 ggtgccaccg gcgcgctgat gagcatccac aactcggtgg gctgcggccc gatcctgaaa
     5041 tacggcaccc aggcgcagaa ggaagcctgg ttgcccgacc tggctgccgg acgggcgatc
     5101 ggctgcttct gcctgaccga gccccaggcc ggctccgagg cgcacaacct gcgcacccgc
     5161 gccgagctgc gcgacggcca gtgggtgatc aatggcgcca agcagttcgt cagcaacggc
     5221 aagcgcgcca aactggccat cgtgtttgcc gtcaccgatc cggagttggg caagaaaggc
     5281 ctgtcggcct tcctggtacc gaccgctacc ccgggtttcg tggtcgaccg taccgaacac
     5341 aagatgggca tccgcgcctc cgacacctgt gcggtgactc tggacaactg ccgcatcccg
     5401 gcggagaacc tcctcggcga acgcggcaag ggcctggcca tcgctctttc caatctcgag
     5461 ggcggccgca tcggtatcgc cgcccaggcc ctcggcattg cccgtgcggc tttcgaagcg
     5521 gcgctggcct acgcccgcga tcgcgtgcag ttcggcaagc cgatcatcga gcaccagagc
     5581 gtggccaaca tgctggccga catgcacacc cgcctgaatg ccgcgcgcct catggtcctg
     5641 catgccgcac gcctgcgcag
//
LOCUS       DATCUN010000127        14048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_211937, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000127 DATCUN010000000
VERSION     DATCUN010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 14048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14048
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211937"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VIO83_05050"
     CDS             complement(<1..140)
                     /locus_tag="VIO83_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8330679.1"
                     /translation="MNVVEIEGVSQRYGRATVLHDLNLSLAPGEVLGLFGHNGAGKTT
                     AM"
     gene            complement(140..1378)
                     /locus_tag="VIO83_05055"
     CDS             complement(140..1378)
                     /locus_tag="VIO83_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HEY8330680.1"
                     /translation="MLIVASAGAVAAVRPITDLPLQADGPNRWRLPAGEYRGSFRIDQ
                     PLQLTCAPGAIFQAEGQGNGLLISAPDVSVQGCTFLDWGADLTAMNAAVFIQPQARGA
                     QVLGNRLQGQGFGIWVDGTQDVSVIGNRIQGDTSVRSQDRGNGIHLYAVHGARVIDNH
                     VRDTRDGIYIDTSNGNLLQGNTLEDLRYGIHYMFANDNQVLGNTTRRTRTGYALMQSR
                     KLTVIGNRSEQDQNYGILMNYITYSTLQDNVVTDVRDGSTGDTMIAGGEGKALFIYNS
                     LFNRIENNRFARSAVGIHLTAGSEDNRIAGNAFIDNQRQVKYVASRLQEWSADGRGNY
                     WSDYLGWDRNGDGLGDVAYEPNDNVDRLLWLYPQVRLLMNSPGIELLRWVQRAFPVMK
                     SPGVMDSHPLMQAPTQKEPA"
     gene            complement(1587..3488)
                     /gene="nosZ"
                     /locus_tag="VIO83_05060"
     CDS             complement(1587..3488)
                     /gene="nosZ"
                     /locus_tag="VIO83_05060"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
                     /protein_id="HEY8330681.1"
                     /translation="MNDTDDKHDGLTRRSFLGTGAVTGAVLAGASALGGAVFTRESFA
                     AAAKDAKSKIHVEPGQLDDYYGFWSGGHQGEVRVMGVPSMRELMRIPVFNVDSATGWG
                     LTNESKRILGEGAKYSNGDCHHPHLSTTDGHYDGKYLFINDKANSRVARIRLDIMKCD
                     KILTVPNVQAIHGLRLQKVPYTKYVFCNAEFIIPHPNDGRNFDLQDRNSFTMFNAIDA
                     EKMEMAFQVIVDGNLDNADMDYTGKYAAATCYNSEKATDLAGMMRNERDWVVVFNIPR
                     IEAAIKAGKFINVDGVKVPVVDGRKSEDGKDSEFTRYIPVPKNPHGCNTSPDGKYFIA
                     NGKLSPTCTIIAIEKLDDLFSGKISDPRGVVVGEPELGLGPLHTTYDGRGFAYTTLFI
                     DSQVVKWNIAEAIRAYGGEKVNYIKQKLDVQYQPGHNHASLTETRDADGQWLVVLSKF
                     SKDRFLPTGPLHPENDQLIDISGDEMKLVHDGPAFAEPHDCVMARRDQIKTKKIWERN
                     DPFFAETVAIAKKDGINLETDSKVIRDGNKVRVYMTSMAPAYGLSEFTVKQGNEVTVT
                     ITNIDMIEDVTHGFVMVNHGASMEISPQQTSSITFIADKPGLHWYYCSWFCHALHMEM
                     VGRMKVEPA"
     gene            complement(3500..5632)
                     /locus_tag="VIO83_05065"
     CDS             complement(3500..5632)
                     /locus_tag="VIO83_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017849335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY8330682.1"
                     /translation="MTRSARLTRHWLTLLVVLLITSFTAQARPYGDIEQQRIERAFPA
                     QDAVSAPDGPFKVRTISTAGKPIGYVFQSLDVVAIPAYSGKPINTLVILDAAGVIRDA
                     YVLEHHEPILLIGIPEVKLHEFTAHYSGVDVRQRVVVGRSSDPKAVTVDAIAGATVTA
                     MVVNEVVMRAAHDVAVWLKLIDGKAATAQKVALIRTDRFDPATWAQLTGNGAIRRLHL
                     TRGQVDAAFKDTAAEGAEAADETFIDLYVADINPPSIGRNLLGEEHYRTLMQGLKPGE
                     QAIAVLGRGLFSFKGSGYVRGGIFDRVQLRQFGNVISFRDLDHQRIDDVYAEGIPAFD
                     EMSVFIVRPQAGFDPGSPWTLELLVRRQTGPVSGTFSSFELPYQMPEQYLERPLPTAA
                     EQAALDEASRPLWLSIWYQKQVQVMVLGVALLLLTAILFLQDSLARRPRLLHWLRRGY
                     LVFTVGFIGWYALGQLSVVNVLTFVHALVQDFRWELFLTDPLIFMLWVFTAASILLWG
                     RGVFCGWLCPFGALQELLNEAARRLKIRQFELPFAVHERLWAIKYLILLVLFGLSLES
                     MATAERFAEVEPFKTAITLKFDRQWWFVLYAVALLVINLFTRKVYCRYLCPLGAALAI
                     PSKFRLFDWLKRRQECGNPCQLCAKECEIQAIHPDGHINANECHYCLDCQMTWHNEKK
                     CPPLINKRKKRNKAAPEQIPVVQINPNA"
     gene            complement(5767..6732)
                     /locus_tag="VIO83_05070"
                     /pseudo
     CDS             complement(5767..6732)
                     /locus_tag="VIO83_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            6935..7396
                     /locus_tag="VIO83_05075"
                     /pseudo
     CDS             6935..7396
                     /locus_tag="VIO83_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927479.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
     gene            7536..8222
                     /locus_tag="VIO83_05080"
     CDS             7536..8222
                     /locus_tag="VIO83_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019649078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8330683.1"
                     /translation="MVLHRVHHQILRSHHLFEPLDEAQLNELMSSSQLLSVDKGEPLF
                     RQGEPADAFYFVIAGAVKIYRLTPDGQEKVFEVIGERQTFAEAMMLMDTPNYVACAEA
                     VCPTQLYRLSNATYMRLLQSNSRLTFALLGKLCVRLHQRVNEIETLSLKNATHRVVRY
                     LLTQLVRLQSTTDCSFELPMAKQLVAGHLSIQPETFSRIIRRLIDENIITQEGRQIAI
                     LDRQRLEQFE"
     gene            8291..8482
                     /locus_tag="VIO83_05085"
     CDS             8291..8482
                     /locus_tag="VIO83_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnrP protein"
                     /protein_id="HEY8330684.1"
                     /translation="MPRCLYCQQDNPPKQAECSNCGMPLPEHADSAPERRQRRFLWFC
                     IGLSVFCAVMIVWLPRHLF"
     gene            complement(8606..9373)
                     /gene="dsbG"
                     /locus_tag="VIO83_05090"
     CDS             complement(8606..9373)
                     /gene="dsbG"
                     /locus_tag="VIO83_05090"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HEY8330685.1"
                     /translation="MIRFKPLPYLLAGVSLLALPMAQAEELPEAIKAVEARGAEVVGR
                     FEAPGGLKGYAARYNGQGMALYLTPDGEHVLIGSLLDAKGDDLTRAHLEKLVYEPLGK
                     EMWTRMENSTWIADGKADAPRIIYMFSDPNCPYCNMFWKQARPWVESGKVQLRHIMVG
                     MLRADSAGKSAALLSAKDPQAALNEHEAAGKASKLKALNKIPAELEEQLTNNLMLMSE
                     LGAQATPAIFYLDDNDRLQQHQGAPQPDALGEIMGPR"
     gene            complement(9370..10185)
                     /locus_tag="VIO83_05095"
     CDS             complement(9370..10185)
                     /locus_tag="VIO83_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8330686.1"
                     /translation="MLTVNIGPLALAVPHVLLLGSLLLATLTGWWVGRRSERNPERQL
                     FRLLLVALFVARLAFVTLYWAYYRDDWLSVIDIRDGGFIAWPGLVAALALGIWWGWRD
                     RELRKPLGIALTVGILSWGFSNLAWHSFEQGTRLPEMALRDNQGRPVALEDYSGQPLV
                     INLWATWCPPCRREMPVLADAQARESDTLFLFVNQGEGEGEINRFLETAGLSLDNVLL
                     DSGGRLGQHVGSTALPTTLFYNADGRQVSSHLGELSHASLARALEKLKEEAAQ"
     gene            complement(10185..11918)
                     /gene="dsbD"
                     /locus_tag="VIO83_05100"
     CDS             complement(10185..11918)
                     /gene="dsbD"
                     /locus_tag="VIO83_05100"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEY8330687.1"
                     /translation="MRILVLLLFLLAPGLALPAGGLFGGGSQDDFLPVEEAFALTVSP
                     QENGATLLHWRIAPGYYLYQQRLQLDGLPPERQPPLPEGEPYSDEFFGDSQIYRDSLE
                     LLIPPGAENSVRLGWQGCADAGLCYPPQTREVSLAGNGTAAAPGLAEDQALVSGLESQ
                     ALAWSLLVFFGLGLLLAFTPCSLPMLPILAGIVVGSGATSRRGFALAATYVVSMALVY
                     AGLGVLAALLGANLQAALQQPWLLTSFAVLFVLLALPMFGLFELQLPVALRDRLQRVG
                     DRQRGGSLAGAGALGVLSGLLVGPCMTAPLAAALLFIAQSGSVLQGGLVLFALGLGIG
                     TPLLLLVTVGQRFLPKPGAWMDRVKVVFGFLFLAAALFVARPLLADWLWLGLWGALLV
                     VLATALLHVAQAVQRQRVLCRASGVLLGLWGAAMLLGAAGGADDPRRPLAIYAGGVAS
                     AISADAHALGFSDPSVLDRELAAAKAQGQWVLIDYYADWCVSCKVMEKEVFGNAEVQA
                     SLDGVRVLRPDVTRSDAASRELLDRYQVLGPPTLLWIGPDGVERRERRITGEVGAGGF
                     LQNWNQTRERG"
     gene            12215..12892
                     /locus_tag="VIO83_05105"
     CDS             12215..12892
                     /locus_tag="VIO83_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8330688.1"
                     /translation="MHVLLAEDDALIASGIVAGLNAQGLIVDHAATAEDAEALLRAAH
                     FDVLILDLGLPDEDGIALLRRLRQQGLELPVLVLTARDAVSDRVTGLQAGADDYLLKP
                     FDLRELAARLHTLMRRMSGRSVNLIEHGRLSYDPSRCEARLDGRLVDLSRREQALLQA
                     LLNNQGRVLSSEQLKDAIYGFADDVESNALNVHIHHLRRKLGSGIVETVRGLGYRLGL
                     AGDEDAP"
     gene            12889..>14048
                     /locus_tag="VIO83_05110"
     CDS             12889..>14048
                     /locus_tag="VIO83_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY8330689.1"
                     /translation="MSLRLRLTLMLGSAFVLLWALAATWMLVDLRSQMMLSLDQRLAA
                     SARMVAGLLVQLPQPVPGDGPPQRLSAEQLGIPNGLACQVSSLRGEVLARSHSAPDQQ
                     LDAEQNGYRDQLIDGVPWRSFTLVQGELRITTADRLDERAALKRSVLLTAALPVAVAL
                     LGSLGLLWLGLGRGLAPLRRIREALTQRSADSLEPLPVEGLPPELLPLVQTQNQLFQR
                     IAQAIERERRLTGDAAHELRSPLTAIKTHLQVARMTEGAAAEQALANAEAGADRLQRT
                     LEQLLLLARVEGSLDFDDGLQCTTAEVARLAIQDASGGDGAPILLQVPADLPDTPLAM
                     PPVLAIAALRNLLENAQRHTAPGSAVTLTLQPEAERVRFEVRDQGPGIAAEHL"
BASE COUNT         2512 a         4564 c         4508 g         2464 t
ORIGIN      
        1 ttcatcgccg tggtcttgcc cgcgccgttg tgcccgaaca accccaggac ttcgccgggg
       61 gccaggctca ggttcaggtc gtgcagtacc gtggcacgcc catagcgctg gctgacgcct
      121 tcgatctcga cgacattcat catgcaggtt ccttctgagt gggggcttgc atcagggggt
      181 ggctgtccat cacacccggc gacttcatca ccggaaacgc acgttgcacc cagcgcagca
      241 actcgatgcc ggggctgttc atcaacagcc gcacctgggg gtacaaccac agcaggcggt
      301 cgacgttgtc gttcggctca tacgccacat cgccgaggcc atcgccgttg cggtcccagc
      361 cgaggtagtc gctccagtag ttgccccggc cgtccgccga ccattcctgc aagcggctgg
      421 cgacgtattt gacctggcgc tggttgtcga tgaaggcgtt gccggcgatg cggttgtctt
      481 cggaaccggc ggtcaggtga atgcccaccg cgctgcgcgc gaagcggttg ttttcgatgc
      541 ggttgaacag cgagttgtag atgaacagtg ccttgccttc gccgccggcg atcatggtgt
      601 cgccggtgga gccgtcgcgc acatcggtga cgacgttgtc ctgcaaggtc gaataggtga
      661 tgtagttcat caggatgccg tagttctggt cctgctcgga gcgattgccg atcaccgtca
      721 gcttgcggct ttgcatcaac gcatagccgg tgcgggtacg gcgggtggtg ttgcccagga
      781 cctggttgtc gttggcaaac atgtagtgga tgccgtaacg caggtcttcc agggtgttgc
      841 cttgcagcag gttgccgttg gaggtgtcga tgtagatgcc gtcgcgggtg tcgcgcacgt
      901 ggttatcgat gacgcgggca ccgtgtaccg cgtacaggtg aatgccgttg ccacggtcct
      961 gcgaacgtac cgaggtgtca ccctggatgc ggttgccgat cacgctcacg tcctgggtgc
     1021 catcgaccca gatgccaaag ccctggcctt gcaggcggtt gcccagcact tgcgcgccac
     1081 gcgcctgggg ctggatgaac acagcggcgt tcatggccgt gaggtcggcg ccccaatcga
     1141 ggaacgtgca gccttggacg ctgacatccg gcgcgctgat cagcaggccg ttgccctgcc
     1201 cttcggcctg gaagatggcg ccgggggcgc aggtcaattg cagcggttgg tcgatgcgga
     1261 acgagccccg gtactcgcca gcaggcaagc gccaacggtt ggggccgtcg gcttgcaggg
     1321 gtaagtcggt gatggggcga acggcggcga ccgctcccgc cgaagccact atcagcatca
     1381 ccaggatgac gagtgatcga gccacgatga tgtcctctta aaggaaatgc ggctaaaagt
     1441 gggagggacg gtgcgacgat tcgacttgct cccgatgagg gtgtgtcagt tgatgcatct
     1501 gtgactgatt caccgccatc gggagcaagt cgaatcgtcg caccgtccct cccacatttg
     1561 ccttgtgtcg cttctcaaac agcgtgtcag gcgggctcga ccttcatccg cccgaccatt
     1621 tccatgtgca gggcatggca gaaccagctg cagtagtacc aatgcagccc gggcttgtcg
     1681 gcaatgaacg tgatcgacga ggtctgctgc gggctgatct ccatgctcgc gccgtggttg
     1741 accatgacaa agccgtgggt cacgtcttcg atcatgtcga tattggtgat ggtcacggtg
     1801 acttcattgc cctgtttgac ggtgaactcc gaaagcccgt aggccggggc catggaggtc
     1861 atgtacacgc gcactttgtt gccgtcgcgg atcaccttgc tgtcggtttc caggttgatc
     1921 ccgtccttct tggcgatggc caccgtttcg gcgaagaatg gatcgttgcg ctcccagatt
     1981 ttcttggtct tgatctggtc gcggcgcgcc atcacacagt catggggttc ggcgaaggcc
     2041 gggccgtcgt ggaccagttt catttcatcg ccggagatgt cgatcaactg gtcgttttcc
     2101 gggtgcagcg ggccggtggg caggaagcgg tccttggaga acttgctcaa gaccaccagc
     2161 cactggccgt cggcgtcgcg ggtttcggtc agcgacgcat ggttgtgacc gggctggtac
     2221 tgcacatcaa gcttctgctt gatgtagttg accttctcgc cgccataggc acggatggcc
     2281 tcggcgatgt tccacttgac cacctggctg tcgatgaaca gcgtggtgta ggcaaagccg
     2341 cgtccgtcgt aggtggtgtg cagcgggccc aggcccagct ccggttcacc gaccaccaca
     2401 ccgcgcgggt cgctgatctt gccgctgaac aggtcgtcga gtttttcgat ggcgatgatg
     2461 gtgcaggtcg gagacagctt gccgttggcg atgaagtact tgccatccgg cgaggtgttg
     2521 cagccgtggg ggtttttcgg caccggaatg tagcgggtga actcgctgtc cttgccgtcc
     2581 tcggatttgc gtccgtccac caccggcacc ttgacgccgt ccacattgat gaacttgccg
     2641 gccttgatcg ccgcttcgat gcgcgggatg ttgaacacca ccacccaatc gcgctcattg
     2701 cgcatcatgc cggccaggtc ggtggccttt tccgagttgt agcaggtggc cgctgcgtac
     2761 ttgccggtgt agtccatgtc ggcgttgtcg aggttgccgt cgacgatcac ctggaaggcc
     2821 atctccattt tttccgcatc gatggcgttg aacatggtga agctgttgcg atcctgcagg
     2881 tcgaagtttc ggccgtcatt ggggtgcggg atgatgaatt cggcgttgca gaacacgtac
     2941 ttggtgtagg gcaccttttg caggcgcagg ccgtggatgg cctggacgtt cggtacggtg
     3001 aggattttgt cgcacttcat gatgtccagg cggatgcgcg ccacccggct gttggccttg
     3061 tcattgataa acaggtactt gccgtcgtag tggccgtcgg tggtggacaa gtgcgggtga
     3121 tggcaatcgc cgttggagta cttggcgccc tcgccgagga tgcgcttgct ctcgttggtc
     3181 aggccccaac cggtggccga atcgacgttg aacaccggga tacgcatcag ctcgcgcatc
     3241 gacgggacgc ccatcacccg cacttcgccc tggtggccgc cactccagaa gccgtagtag
     3301 tcgtcgagtt ggccgggttc cacatggatt ttcgatttgg cgtccttggc cgccgccgcg
     3361 aaggattcac gggtaaacac cgcgccgccg agggccgacg cacccgccag tacggcgccg
     3421 gtcaccgcac cggtgccgag gaaactgcgc cgggtcaggc cgtcatgttt atcgtctgta
     3481 tcgttcatgg gttgctcctt caagcgttgg gatttatctg cactacggga atctgttcgg
     3541 gcgccgcctt gttgcgcttc ttgcgtttgt tgatcagcgg cgggcatttt ttttcgttgt
     3601 gccaggtcat ctggcagtcg aggcagtagt ggcattcgtt ggcgttgata tggccgtccg
     3661 ggtggatcgc ctggatctcg cattccttcg cgcacagctg gcacgggttg ccgcattcct
     3721 ggcgccgctt gagccaatcg aacaggcgga atttgctggg gatcgccagg gcggcgccga
     3781 ggggacagag gtagcggcag tagactttgc gggtgaacag gttgatcacc agcaacgcca
     3841 cggcgtagag cacgaaccac cactggcggt cgaacttgag cgtgatggcg gtcttgaacg
     3901 gttccacttc ggcaaagcgt tcggcggtgg ccatggattc cagggagagg ccgaacagca
     3961 ccagcaggat caggtacttg atcgcccaca gccgttcatg caccgcgaag ggcaattcga
     4021 actggcggat cttcaggcgg cgcgcggcct cgttgagcag ttcctgcaag gccccgaacg
     4081 ggcacagcca gccgcagaac accccacgcc cccacaacag gatgctggcg gcggtgaaga
     4141 cccagagcat gaagatcagc gggtcggtga ggaacagttc ccagcggaag tcctggacca
     4201 gcgcatgcac gaaggtcagc acattgacca ccgacaactg ccccagcgca taccagccga
     4261 tgaaccccac ggtaaacacc aggtagccgc ggcgcagcca gtgcagcagg cgcgggcgcc
     4321 gggccaggct gtcctgcagg aacaggatcg cggtaagcag cagcagcgcg acgccgagca
     4381 ccatgacctg cacctgtttc tggtaccaga tgctcaacca cagtgggcgg ctggcctcat
     4441 ccagcgcggc ttgttcggcg gcggtgggca gcggccgttc caggtactgt tcgggcatct
     4501 ggtaaggcaa ttcgaagctg ctgaacgtgc cgctgaccgg gccggtctgg cgccgcacca
     4561 gcagttccag ggtccagggc gagccggggt cgaagccggc ctgcgggcgc acgatgaata
     4621 ccgacatctc gtcgaaggcc gggatgccct cggcgtagac atcgtcgatg cgctggtggt
     4681 cgaggtcgcg gaagctgatc acgttgccga actggcgcaa ctgcacccgg tcgaaaatcc
     4741 cgccgcgcac ataacccgaa cccttgaacg aaaacagccc acggccgagc acggcgatgg
     4801 cctgttcgcc gggtttgagc ccctgcatca gggtgcggta gtgctcttcg cccagcaggt
     4861 tgcggccgat gctcggcggg ttgatgtcgg ccacatagag gtcgatgaag gtctcgtccg
     4921 cagcctcggc gccttcggcg gcggtgtcct tgaaggccgc gtcgacctgg ccccgggtca
     4981 aatgcaggcg gcggatcgcg ccattgccgg tcaattgtgc ccaggtggcg gggtcaaacc
     5041 ggtcggtgcg gatcagcgcg actttctgcg cggtcgcggc cttgccgtcg atcagcttga
     5101 gccacaccgc cacgtcatgg gccgcccgca tcaccacttc attgaccacc atcgcggtca
     5161 cggtcgcgcc ggcaatcgcg tccaccgtca ccgccttcgg atcgctggaa cgcccgacca
     5221 ccacgcgctg gcgcacatcg acgccggaat agtgcgcggt gaattcgtgc agtttgacct
     5281 cgggaatacc gatcaacagg atcggctcgt ggtgttccag cacgtaggca tcgcggatca
     5341 ccccggcggc gtcgaggatc accagggtgt tgatcggctt gcccgaatac gccgggatcg
     5401 ccaccacatc caggctctgg aacacgtagc cgatgggctt gccggcagtc gaaatggttc
     5461 gcaccttgaa cggcccgtcg ggtgcggaga ccgcatcctg ggcggggaaa gcgcgttcga
     5521 tacgctgctg ttcgatgtcg ccgtaaggcc gggcctgggc ggtgaagctg gtgatgagca
     5581 acaccaccag cagcgtcagc cagtggcgtg tcaagcgcgc ggagcgagtc atggagtccc
     5641 ttgaggcgtc gggctgaaga tgggacggat ggtaggggtg gtgatggcag gtggcccttg
     5701 accagaatca agtctggccg gtaggtagtg cggcagatac gcacagctgg cgtgttgtca
     5761 caccccttac ttgagcttca ggcgccgcgc cagcttgtgc aggttgctcg cgtccacgcc
     5821 gagcaaacgc gcggcactcg cccaattgcc ggcgcagtgt tccagggcgt ggcgaatgtt
     5881 ccagcgctgg cactcgtcca ccgccaccgc caagggttgg aaggcctcga tcgatgacgc
     5941 gggcatggct cgttccaccg cgacggcggc cgtgtccagg cccagagcgt gcggttccag
     6001 gcggatcacc gaactgcgcg gctcgccacg actgagctgc ttcaacgcgg cgcgactgat
     6061 cacatgctcc agctcacgca cattgcccgg ccaggcgtac tcgagcagcg cctgttgcgc
     6121 cgccggcgac agttgcaggt catgcaagcc caggcgctcg cggttgagct cagcgaaatg
     6181 ccgcgccagc agcaacacat cctcgccacg ctcgcgcaac gccgggatcg gcagcgggta
     6241 caccgacagc cggtgataca ggtcggcgcg aaacaggccg tcacgcacgc ggtcgggcag
     6301 gtggcgattg gtggcggcga tgatgcggac atccacatgc aatggccgat cggcgcccag
     6361 gcgttggatc tcgccattct ccagggtgcg cagcagcttg gcctggatgc tcaagggcaa
     6421 ttcgccgacc tcatcgagga acaaggtgcc gccgttggcc gcatcgaacc gcccggcgcg
     6481 gtcgtgggtg gcgccggaga aggcgccctt gacgtgaccg aacagctcgc tttcggccaa
     6541 cgcctccggc agcgccgcac agttgacctg caccagaggc ttgttgccgc gttgcgacag
     6601 gcgatgcagg cgccgcgcga acagctcttt gccgaccccg gtttcgccga gcaacaacac
     6661 cggcaactgc gtttcggcca gcagcgccag ctcgtcgagc aactcctgca acaccgcgct
     6721 ctggccgagg atgccgtcga gcggcgcggt catttcgacc ctcctggggt tcagagcacc
     6781 ctggaagcgt gtggtcatga ttaccttact ccgggataaa tcgttacttt tcaatgcgtt
     6841 aaaattggca cgcagcctgc aatcactggt tcatacccgt actgccgatg aggaaagccc
     6901 gatgaccgtg agcgactgga acacccaaac ccaggaccac ttgatcgacc atctgctggc
     6961 gcgctaccac gcacgtcatc gcgagcaatt gccggaactg gtccgcatgg cgagccgcgt
     7021 cgaacaggtc cacggtcagc acctgcaatg ccccaacggc ctggccgacc tgctgctcga
     7081 catgcagcag gaactggaga gccatatgct caaggaggaa caggtattgt tcccgatgct
     7141 gcaaatgggc atggggccgc aggcgtcgac gccgatccag gtgatgcgct acgagcacga
     7201 ccaacacgcc gaggccctgg agcaattgtt gagcctgacg aaccagatca ccccgccggc
     7261 cgaggcctgt aatacctggc gcagcctgta ccgcgagctg agcgcctttc atgccgacct
     7321 gcgcgaacac atccgcctgg agaacgacat attgtttgcc cgggccgcgc ccagcctcgc
     7381 cgtggcccgc gcatagcgca tagcgcactt tcacaagcgt tcactgcgac gatcgtgcag
     7441 cgttgcctgt gcgctgccct tgtcgtagat caagtacggc cgacgccaac aggattaacc
     7501 ttgggtatcc cttcccgata ttccccgagg tcaagatggt gctccatcgt gttcatcacc
     7561 agatcctgcg cagccaccac ttgttcgaac ccctggacga ggcgcagttg aacgagctga
     7621 tgagcagcag ccaattgctc agtgtcgaca aaggcgaacc gctgttccgc cagggcgaac
     7681 cggccgatgc gttctacttc gtgatcgccg gcgcggtgaa aatctaccgc ctcaccccgg
     7741 acggccagga gaaggtgttc gaggtgattg gcgaacggca gaccttcgcc gaagccatga
     7801 tgctgatgga tacgcccaac tacgtagcgt gcgccgaagc ggtgtgcccc acgcagttgt
     7861 accggctgtc caatgccacc tacatgcgtt tgctgcagag caacagccgc ctgaccttcg
     7921 ccctgctcgg caagttgtgc gtgcgcctgc accagcgggt caacgagatc gaaaccctgt
     7981 cgctgaaaaa cgccacccac cgtgtggtgc gctacctgct cacgcaactg gtgcgcttgc
     8041 aaagcaccac cgactgcagc ttcgagctgc cgatggccaa gcagctggtg gccgggcacc
     8101 tgtcgatcca gccggagacg ttttcgcgga tcattcggcg actgatcgat gagaacatca
     8161 tcacccagga aggccggcag atagcgatcc tcgatcgcca gcggctcgag cagttcgagt
     8221 gagttgatcg tcgtcaaagc ctgcgatttt tccgccgtgt caggctttac cgaccgagag
     8281 gagcgccgcc atgcccagat gcctgtactg ccaacaggac aacccgccga agcaagccga
     8341 atgcagcaac tgcggcatgc cgctgccgga acacgccgac agcgccccgg agcgccggca
     8401 gcggcgcttc ctgtggttct gcatcggtct aagcgtcttc tgcgcggtga tgatcgtctg
     8461 gctgccacgc catctgttct gatggcgccc cacccaatgg tgtctacagc ggatagtcgc
     8521 ttcgcagtga aacgccgccc cgtgctgaag cggatgtgcc gcgaaacagc tcagcgacca
     8581 gccttccctt gcccacagca atccttcagc gtggccccat gatctctccc agcgcatccg
     8641 gctgcggtgc accctggtgc tgctgcagac ggtcgttgtc gtcgagatag aagatcgccg
     8701 gagttgcctg ggcgcccagt tcgctcatga gcatcaggtt gttggtgagc tgttcttcga
     8761 gttctgccgg gatcttattc agcgccttca gcttgctcgc cttgccagcc gcttcgtgct
     8821 cgttgagcgc tgcctgagga tccttggcac tgagcagcgc ggcggatttg ccggcgctgt
     8881 cggcgcgcag catgccgacc atgatgtgcc gcaactgcac cttgccggac tcgacccagg
     8941 gtcgggcctg cttccagaac atgttgcagt acggacagtt cgggtcgctg aacatgtaga
     9001 tgatgcgcgg cgcgtcggcc ttgccgtcgg cgatccaggt gctgttctcc atgcgcgtcc
     9061 acatttcctt gcccaggggt tcgtagacca gcttctccag gtgcgcgcgg gtcaggtcat
     9121 cgccttttgc atcgagcagg ctaccgatca gcacgtgctc gccatccggg gtgagataca
     9181 gcgccatgcc ctgcccgttg taacgcgcgg catagccctt gagcccgccg ggtgcctcga
     9241 aacggccgac gacctcggcg ccacgcgcct cgacggcctt gatcgcctcc ggcaattcct
     9301 cggcctgcgc catgggcagg gccagcaggc tgacgccggc cagcaggtag ggtaacggct
     9361 tgaatcgaat cactgcgcag cttcctcttt cagtttttcc agcgcgcgcg ccaggctggc
     9421 gtgcgagagt tcaccaagat ggctgctgac ctggcggcca tcggcgttgt agaacagcgt
     9481 ggtcggcagc gccgtggagc ccacgtgctg accgaggcgc ccgccactgt cgagcaggac
     9541 gttatcgaga ctcagccctg cggtttccag gaagcggttg atctccccct ccccctcgcc
     9601 ctgattgacg aacagaaaca gggtgtcgct ttcccgtgcc tgggcgtcgg cgagtaccgg
     9661 catctcgcgc cggcacggcg ggcaccaggt cgcccagaga ttgatgacca gtggctgacc
     9721 tgaataatcc tccagcgcca ccggacgccc ctggttgtcg cgcagggcca tttccggcag
     9781 ccgcgtgccc tgctcgaagg aatgccaggc caggttactg aagccccagc tgaggattcc
     9841 caccgtcagc gcgataccca gcggcttgcg cagttcgcgg tcgcgccagc cccaccagat
     9901 gcccaacgcc agcgcagcga ccagcccggg ccaggcgatg aagccaccgt cacggatatc
     9961 gatgacgctt agccagtcat cccggtagta cgcccagtac agcgtgacaa aggccaggcg
    10021 agccacgaac agcgccacca gcagcaggcg aaacagctgc cgctccgggt tgcgctcgct
    10081 gcggcggccg acccaccagc cggtcagggt cgccagcagc aggctgccca gcagcaatac
    10141 atggggcacc gccagggcca gcggcccgat attcacagtc agcatcaacc tcgctcccgg
    10201 gtctggttcc agttctgcag aaagcccccg gcaccgacct cgccggtgat acggcgttcg
    10261 cggcgctcga caccatccgg gccgatccac agcagcgtcg gcgggccgag cacctggtaa
    10321 cggtccagca attcgcggct ggcggcgtcg ctgcgggtca cgtcggggcg cagcacgcgc
    10381 acgccgtcca ggctagcctg cacctcggca ttgccgaaga cctccttctc catgacctta
    10441 caggagacgc accagtcggc gtagtaatcg atcagcaccc actggccctg cgctttggcc
    10501 gctgccagct cacgatccag cacggacgga tcgctgaaac cgagcgcgtg agcgtcggcg
    10561 gagatggcgc tcgccacgcc gccggcatag atcgccagcg gccggcgtgg atcgtccgcg
    10621 ccgccggcgg caccgagcag catcgccgcg ccccacagcc ccagcagaac gccgctggcc
    10681 cgacaaagca cgcgctggcg ttgcactgcc tgcgccacat gcagcaaggc ggtggcgagc
    10741 accaccagaa gagcgcccca caggcccagc cacagccagt cggccagcaa cggacgcgcc
    10801 acgaacagcg ctgccgccag gaagagaaag ccgaacacca ccttgacccg atccatccag
    10861 gcgcccggct tgggcagaaa gcgctggcca accgtcacca gcaagagcaa cggcgtgccg
    10921 atgccgaggc ctagcgcgaa cagcaccagg ccaccctgca gcacactgcc gctctgtgcg
    10981 atgaacagca gcgcggcggc cagtggcgcg gtcatgcagg ggccgaccag caggccggag
    11041 agcacgccca gcgcgccagc tccggcgagg ctgcccccgc gctgacgatc accgacacgc
    11101 tgcagacgat cgcgcagcgc caccggcaat tgcagctcga acaaaccgaa cattggcagc
    11161 gccagcaaca cgaacagcac ggcgaaactg gtcagcagcc agggctgctg cagcgccgcc
    11221 tgcaggttgg cgccgagcag cgccgccagt acaccgagcc cggcgtagac cagcgccatg
    11281 ctgactacgt aggtcgccgc cagggcaaaa ccacggcgcg aggtggcacc gctgccgacc
    11341 acgatgcccg ccaggatcgg cagcatcggc agcgaacagg gggtgaaggc cagcagcaag
    11401 ccgaggccga agaagaccag caggctccag gccagtgcct gactttcgag gccgctgacc
    11461 agcgcctgat cttcggccag cccaggcgct gccgccgtgc cgttgccagc caacgacacc
    11521 tcgcgggtct gcggcggata gcagagccct gcatcggcgc agccctgcca gccgagacgc
    11581 acactgttct ccgcgcccgg cgggatcagc agttcgaggc tgtcgcggta gatctgcgag
    11641 tcgccgaaga actcgtcgct gtagggctcg ccctcgggca gcggcggctg gcgctccggc
    11701 ggaaggccgt ctaactgcag gcgctgctga tagaggtaat agcccggtgc gatccgccag
    11761 tgcagcagcg tcgcgccgtt ctcctgcggg ctgaccgtca gcgcgaacgc ctcttccacc
    11821 ggcaggaaat catcctgcga gccaccgcca aacagcccac cggccggcag cgccaatccg
    11881 ggcgccaaca gaaacagcag aagaaccaat atgcgcatga gagtcctggc cgagaaatga
    11941 ctgccacgca gcatgccgcg acgggattaa cgcgccatta acaggccgct gaaaaacgta
    12001 ggcgaggccg ccagcgcgag gctgggcgcc cccggcaaaa aacaggcgaa aaagcgcagt
    12061 ttacgaacgg taaaagagca ttttgagcct gtttttaacg ctgcgatggc aacgcaggta
    12121 gtttttcaac ggcctgccaa cgccagcgca cgggctgcat aacgttgcct taatcgctaa
    12181 ggtccaccct tgagcttttt ctgcggaccc tcccatgcac gtgctgcttg ccgaagacga
    12241 cgcgctgatc gccagcggta tcgtcgccgg gctcaacgcc cagggcctga ttgtcgacca
    12301 cgccgcgact gcggaggatg ccgaagccct gctgcgtgcg gcgcacttcg atgtgctgat
    12361 cctcgacctc ggcctgcccg acgaagacgg catcgccctg ctgcgacgcc tgcgccaaca
    12421 gggcctggaa ctaccggtgc tggtgctcac cgcgcgcgat gcggtcagcg accgggtcac
    12481 cggcctgcag gcgggtgccg acgactacct gctcaagccg ttcgacctgc gcgagctggc
    12541 cgcgcgcctg cacacgctga tgcggcgcat gtccggccgc agcgtcaacc tcatcgaaca
    12601 tggccggttg agctacgacc cgagccgctg cgaagcacgc ctggacggcc gcctcgtcga
    12661 cctgtcgcgc cgcgaacagg cactgctgca ggcgctgctc aacaaccagg gccgggtgct
    12721 cagcagcgag cagctcaagg acgcgattta cggtttcgcc gatgatgtcg agagcaacgc
    12781 gctgaacgtg catatccacc atctgcgccg caagctcggc agcggcatcg tcgaaaccgt
    12841 gcgcggcctc ggctatcgcc tcggcctcgc aggcgacgag gacgcgccat gagcctgcgt
    12901 ctgcgcctga ccctgatgct cggctcggcc ttcgtgctgc tctgggcatt ggctgcgacc
    12961 tggatgctgg tcgatctgcg cagccagatg atgctctcgc tcgatcagcg cctggcggcc
    13021 tcggcgcgca tggtcgccgg tctgctggtg cagttgccgc agccggtgcc cggcgacggc
    13081 ccgccgcagc gtctgagcgc cgagcagctg ggcatcccca acgggctggc ctgccaggtc
    13141 agctcgttgc gtggcgaggt gctggcgcgc agccacagtg cgcccgacca gcagctggat
    13201 gccgagcaga acggctatcg cgaccagctg atcgacggcg tgccctggcg cagtttcact
    13261 ctggtgcaag gcgagctgcg catcaccacg gccgaccgtc tggatgagcg tgccgcgctg
    13321 aagcgctcgg ttttgctgac ggcggcgctg ccggtggcgg tcgccctgct tggcagcctg
    13381 ggtctgctct ggcttggcct gggccgtggg ctggcgccgt tgcgcaggat tcgcgaggca
    13441 ctgacccaac gcagcgccga ctccctagaa ccgttgccgg tcgaaggcct accgccggag
    13501 ctgctgccgc tggtgcagac gcagaaccag ctgttccagc gcatcgccca ggcaatcgaa
    13561 cgcgaacgcc ggctgaccgg cgacgccgcc cacgaactgc gcagcccgct gacggcgatc
    13621 aagacccacc tgcaggtagc acgcatgacc gaaggcgccg ccgccgaaca ggcactggcc
    13681 aacgccgagg ccggcgccga tcgcctgcag cgcacgctgg aacagctgct gttgctggcg
    13741 cgggtagaag gcagtctgga tttcgacgat ggcttacagt gcacgaccgc cgaggtcgcg
    13801 cggctggcga ttcaggacgc cagtggcggt gacggcgcgc caatcctgtt gcaggtcccg
    13861 gcggacctgc ccgacacgcc gctggccatg ccgccggtgc tagccatcgc cgcgctgcgc
    13921 aacctgctgg agaatgccca gcgccacacc gcgccgggca gcgccgtcac gctaaccctg
    13981 cagcctgaag ccgagcgagt acgcttcgaa gtacgcgacc agggcccggg catcgccgcc
    14041 gagcatct
//
LOCUS       DATCUN010000128         7630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_61419, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000128 DATCUN010000000
VERSION     DATCUN010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61419"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VIO83_05115"
     CDS             complement(<1..413)
                     /locus_tag="VIO83_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330690.1"
                     /translation="MRPERGFALVAALFVMVIVAVVVATMSRLSVTQNASLDLAIQQA
                     RAYQAARAGLEWGLHQLMAESNTTGVCPVGNPSMSGSGLSEFSVSLVCVKLPCAAATC
                     AQTYTEGIRTFNLYRLTATAYNGSPDSRVDYAWRR"
     gene            complement(403..1266)
                     /locus_tag="VIO83_05120"
     CDS             complement(403..1266)
                     /locus_tag="VIO83_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY8330691.1"
                     /translation="MRALIPAAPARQRGFTLVELILVIVLSAVVGAMVSSVLSRPLEG
                     FVAQSRRAELVDIGAGALNRMQRDIRMAVPGSLRISGDGQALELLLIHSGGRYLPNRA
                     DGVSLNFSAAPDAQCNGPQVAGVPACDVLQVFDPDLAPPAAGWLVVASNPFSWVVSNP
                     GNITPTGTSFTRLADSVDYPGISLIRVTPPGGNFAFAAASAQRRIYFADRVVGYRCSG
                     GQLLRYEHDQLQAALPATLPATGRLQADRVESCSFGYASASGLATLRLTIGAAGDSVR
                     LAQQVQVNNAP"
     gene            complement(1254..1751)
                     /locus_tag="VIO83_05125"
     CDS             complement(1254..1751)
                     /locus_tag="VIO83_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY8330692.1"
                     /translation="MKRQRGVTLVELVIAIVIVGIAAAALYTAMASITGRSADPMLRQ
                     QSLAIAEAYLEEILAQDFLDPTSHDQCPAAPVARAAFDNVCDYAGLVDSGAEDASGTL
                     VPGLQDYRVSVAVTPQPFNSLTVAAALLVTVTVEAPDDQTLTLSGWRTCYGEYDGTGA
                     YACVP"
     gene            complement(1748..2194)
                     /locus_tag="VIO83_05130"
     CDS             complement(1748..2194)
                     /locus_tag="VIO83_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY8330693.1"
                     /translation="MHSRGFTLVELILTIVIVGILAAVVGPRFFERSTFDERLYAEEV
                     RAGLRHAQKLALAGGCRIRFSLDTTGYQLLRDADCEGSGNTFSPSVPDPSTGQAPYAG
                     TPPAGTALSASFNVDFDALGRPSQAVSVTLGSGHAVRVEAETGFVP"
     gene            complement(2199..2660)
                     /locus_tag="VIO83_05135"
     CDS             complement(2199..2660)
                     /locus_tag="VIO83_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY8330694.1"
                     /translation="MQQQRGFTLVELIMVIVILGILAAFALPRFANLGKDARIAAVQG
                     AAASIRSASAIGHTGWLVQGGTNNEVTLEGATVLIYGGYPQARVTLAGAVGIAGAAGI
                     DSGSYQIATTATTATIRPLGVASTASCYVQYTQANLAGTVPPTIVIQTGGC"
     gene            complement(2800..3252)
                     /locus_tag="VIO83_05140"
     CDS             complement(2800..3252)
                     /locus_tag="VIO83_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY8330695.1"
                     /translation="MKKQQSGFTLIELIMVIVVLGILAAFALPKFANLGSDARRASIE
                     GVTASMKSASAIIHSAYLAKNDSTVTEISAEGVDVLLEETYPASATDTTVGIIEAAGI
                     SKSEFTVTYGTGTVTVAAKGASGACQVVYVDAADANTPPTITPGTGGC"
     gene            complement(3299..3826)
                     /locus_tag="VIO83_05145"
     CDS             complement(3299..3826)
                     /locus_tag="VIO83_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8330696.1"
                     /translation="MKRQKGFTLIELVVVIVILGVLAAVALPRFMNATEDAHTAAVNG
                     TGGALAAGVALVRSQWELNRVQGIANPNNNVAGFGEGNMDVNGTGWPVGANVAVGTTL
                     AAPDTNARCIQIWEGVLQGSAPSVAGGATADYQAAVNAGTGCTYTYRLDGRTVAPLRT
                     IVYDATTGAVTTNAD"
     gene            complement(3857..4369)
                     /locus_tag="VIO83_05150"
     CDS             complement(3857..4369)
                     /locus_tag="VIO83_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330697.1"
                     /translation="MKPLPARDERLLLVAPLQRRGWRLLTLILANVIFISLWHIAVDS
                     ETKAMNTTAEHLARSLDALAAENMAQGRPLDAGWATGNPFVLLRGRQDNYCGELAQGQ
                     TPSRGCWYFLPQQSRVLYRARFRGWRSGGGEGVQVWQLVRLPLQTMDTAKAERLTAVE
                     LRAVDWTDPK"
     gene            complement(4366..5607)
                     /locus_tag="VIO83_05155"
     CDS             complement(4366..5607)
                     /locus_tag="VIO83_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8330698.1"
                     /translation="MANFRFSGRDAQGRKISGSRQAASAEALAGELLAERITPLSIDA
                     LAEKAAGGGDVLRQLNKLLGRERVELEELIIFCRQMYSLNKAGVPIIRAIGGLAESAR
                     NPYFREVLQTLRSDLEGGTAMAASLGNHPRVFNPLFVSMISVGENTGQLDQAFRQLAG
                     YLEMERETRKRIKQATRYPLFVLIAMSVALAVINLLVIPAFSKVFAQFHAELPWPTRV
                     LIGFSEFMQDWWWLLGMAIAGSIVAFVRWIATDAGALVWDRYKLKLPIVGPIFERIAL
                     ARFTRTFAMMYRAGLPLLQTLGINSHTVGNRHIGSAITAMRAGVERGEALTRGAAASG
                     LFTPLVLQMMAVGEETGALDDLFVEVADFYEQEVDYDLKRLADAIEPILIVAMGVMVL
                     ILALGVFLPMWELSAAAKGVK"
     gene            complement(5607..7328)
                     /locus_tag="VIO83_05160"
     CDS             complement(5607..7328)
                     /locus_tag="VIO83_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY8330699.1"
                     /translation="MDSVDLRQRKVRLGDLLVQAGLISEGQLQLALQEQKRSGAKLGR
                     AVVDLGFVSEQKLLEALSRQLEIPYVDLRQFKLDAVLIQRLPETLARRFRAILLVEES
                     DGLLVGMGDPLDLYALDEMSRLLRQPLKPAVVRESDLLDNLDRFYRRTGEIAELAGQL
                     DERLEDSDFDFSGLSLDNSDAPVVRLLQMLFEDAVQMKASDIHIEPDEKVLRLRQRID
                     GVLNEQLIKETRIASALVMRLKIMAGLDISEKRLPQDGRFNIRVKNHSLDVRVSTMPV
                     QFGESVVLRLLDQSAGVFSLDRTGMPADMLARFRTLLQRPHGIVLVTGPTGSGKTTTL
                     YAGLSELNSPEKKIITVEDPVEYRLPRISQVQVNPKIELTFARVLRAALRQDPDIVLV
                     GEIRDRETAEIALRAALTGHLVLSTLHTNDAMTSPLRLIDMGAEPFLVANALNAVLAQ
                     RLVRRVCEHCREEHLPEERERHWLEGISGQPLAGRRFVRGSGCHQCHNTGYLGRIGVY
                     ELLEIDAPLAAALRRHDAQGFIDAARAQPYYRPLAASALDFALAGLTSVEEVLKVSAS
                     LSDERIG"
     gene            complement(7328..>7630)
                     /locus_tag="VIO83_05165"
     CDS             complement(7328..>7630)
                     /locus_tag="VIO83_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330700.1"
                     /translation="VATLAAHLPPLAGDPAYHALLAASYQQTGQWRESAQLYRNLVLL
                     RPEQSAWQLGLGIALEQLGERAAAAQHYRQALQGQGLDDGSRRYARERAQALGGAS"
BASE COUNT         1307 a         2522 c         2570 g         1231 t
ORIGIN      
        1 aaccgtcgcc aggcatagtc cacccggctg tccgggctgc cgttgtaggc cgtagcggtc
       61 agacggtaca gattgaaggt gcggataccc tccgtgtagg tctgcgcgca ggtggccgcg
      121 gcacagggca gcttgacgca gacgaggctc acgctgaact cgctcaggcc gctgccggac
      181 atggatggat tacccacagg acagacgccc gtggtattgc tctcggccat caactgatgc
      241 agcccccact ccaggccggc acgggcggcc tggtaggcgc gggcctgctg gatggccagg
      301 tcgaggctgg cgttctgggt gaccgacagg cggctcatgg tcgccaccac cacggcgacg
      361 atgaccatca cgaacagcgc cgccaccagg gcgaatccac gttcagggcg cattgttcac
      421 ctgtacctgc tgggccagcc ggacgctatc gcccgccgcg ccgatggtca gacgcagggt
      481 ggccagcccg ctggcgctgg cgtagccgaa actgcaggac tcgacgcggt cggcctgcag
      541 acgcccggtg gccggcagcg tcgcgggcag ggcggcctgc agctggtcgt gctcgtagcg
      601 cagcagttgg ccgccgctgc agcgatagcc gaccacccgg tcggcgaagt agatgcgccg
      661 ctgcgccgag gcggcggcaa aggcgaaatt gccgcccggc ggcgtcacgc ggatcaggct
      721 gatgccggga tagtcgaccg agtcggccag gcgcgtgaag ctggtgcccg tcggcgtgat
      781 gttgcccgga ttgctcacga cccagctgaa cgggttgctg gccaccacca gccagccggc
      841 ggccggcggc gcgagatccg gatcgaacac ctgcagcaca tcgcaggccg gcacgcccgc
      901 cacctgcggg ccgttgcact gcgcatccgg cgcggcggaa aagttcaggc tgacgccgtc
      961 ggcacgattg ggcaggtagc gcccgccgct gtggatcagc agcagctcca gcgcctggcc
     1021 atcgccgctg atccgcagcg aaccgggaac cgccatgcgg atatcgcgct gcatgcggtt
     1081 gagggcgccg gcgcctatgt ccaccagttc ggcgcggcga ctctgggcga cgaagccttc
     1141 cagcggacgc gacagcacgc tgctgaccat cgccccgacc accgcactga ggacgatgac
     1201 caggatcagc tcgaccaggg tgaagccgcg ctgacgggcc ggggcagccg gaatcagggc
     1261 acgcatgcgt aggctcccgt gccgtcgtac tcgccatagc aggtgcgcca accggaaagg
     1321 gtcagcgtct ggtcgtccgg cgcctcgacc gtcaccgtca ccagcagcgc cgcagcgacg
     1381 gtcaggctgt tgaagggctg cggcgtcacg gcgacgctga cccgatagtc ctgcaagcca
     1441 ggcaccagcg tgccactggc atcctcggca ccgctgtcga cgagaccggc gtagtcgcag
     1501 acgttgtcaa aggcggcgcg cgccacaggt gcagccgggc actgatcgtg actggtcgga
     1561 tcgaggaagt cctgggcgag gatttcctcc agataggcct cggcgatggc cagactctgc
     1621 tggcgcagca tgggatcggc cgagcggccg gtgatgctgg ccatggcggt atacaaggcc
     1681 gccgccgcga tgccgacgat gacgatggcg atcaccagtt cgaccagggt gacgccgcgc
     1741 tgacgcttca cggcacgaac ccggtttccg cctcgacacg cacggcatgg ccactgccaa
     1801 gagtgacact gaccgcctgg ctgggccggc cgagggcgtc gaaatcgacg ttgaatgagg
     1861 ccgacagcgc cgtaccggcc ggcggtgtgc cggcgtaggg tgcctgcccg gtggacggat
     1921 cgggcacgct ggggctgaag gtgttgccgc tgccctcgca gtcggcatcg cgcagcagct
     1981 ggtagcccgt cgtgtcgagg ctgaagcgaa tgcggcagcc gccggccagc gccagcttct
     2041 gcgcatggcg cagcccggcg cggacttcct cggcgtacag ccgctcgtcg aaggtcgagc
     2101 gctcgaaaaa acgcggcccc accaccgcag cgaggatgcc gacgatcacg atcgtcagga
     2161 tcagctcgac cagggtgaag ccgcgggaat gcatggggtc agcagccgcc ggtctggatg
     2221 acgatggtgg gcggaacagt gcccgctagg ttggcttgcg tgtattgcac atagcaactc
     2281 gcggtagagg caacacccaa cggccggatg gtggccgtgg tggccgtggt cgctatctgg
     2341 tagctgccgc tatcgatacc ggcggcaccg gcaatgccga cggctccggc caaggtcacg
     2401 cgcgcctgag gatacccccc gtagatcaac accgtggcgc cctccagcgt cacctcgttg
     2461 ttggttccgc cctgaaccag ccatcctgta tggccaatag ccgaggccga gcggatcgac
     2521 gcggcggcgc cctgtacggc ggcgatgcga gcgtccttgc ccagattggc aaagcgcggc
     2581 aaggcaaagg cggcgaggat gccgaggatg acgatgacca tgatcagctc tacgagagtg
     2641 aagccacgtt gttgttgcat ggatacggtc cttgtcctga cgacttggcc agctccgagc
     2701 cggcagttgg gtaattagtc gacaggtgcc cggaggcacc tgtcttgttt ggagcgctaa
     2761 agcagagtgg aaatggggtc tatccctttt tctccgaact cagcagcccc cggttccggg
     2821 agtgatagtc ggcggagtat tggcatccgc agcatcaacg taaacgacct gacaggcgcc
     2881 acttgcaccc ttggcagcca cagtcactgt gccagtgcca taggtcacgg tgaattcgct
     2941 tttggaaatt ccagcggctt cgatgatgcc tacagtggta tcggtagcgg aagccggata
     3001 ggtttcctcc agaagaacat ccaccccttc agcagagatt tcagtcaccg tggaatcgtt
     3061 cttggccaga tatgccgagt gaatgatggc cgaggccgac ttcatcgagg cggtaacgcc
     3121 ctcaatgctg gcacggcggg catcggagcc caggttggca aacttcggca acgcaaacgc
     3181 cgccagaatg cccagcacca caatgaccat gatcagctcg atcagggtga agccactttg
     3241 ctgttttttc atgattgcag tcctttcgga taattatcta agttagaggt agaacaaatc
     3301 agtcggcgtt ggtggtcacg gcgccagtgg tggcgtcata gacgatggtg cgcagcggag
     3361 ccacagtgcg gccgtctagg cggtaggtgt aggtgcaacc ggtaccagcg ttgaccgctg
     3421 cctgataatc cgcggtagct ccaccggcca ctgaaggcgc cgacccctgc aacacccctt
     3481 cccagatctg tatgcatcgg gcattggtat ccggagcagc cagagtggta ccgactgcaa
     3541 cgttggcgcc caccggccag ccagtgccgt tcacgtccat gttcccctca ccgaaaccgg
     3601 ccacgttgtt gttcgggttg gcaatgccct gcacccggtt caactcccac tgcgaacgca
     3661 ccagcgccac accggcagcc agcgcaccgc cagtaccatt cacagcagcg gtatgcgcat
     3721 cctcagtcgc attcatgaaa cgcggcagcg ccaccgcggc gaggacgccg aggatcacga
     3781 tcaccaccac cagctcgatc agggtaaagc ccttctgtcg tttcatttcc ttgcatctcc
     3841 tgttgccctg cgggcgtcat ttcggatcgg tccagtccac cgcccgcagc tccacggcgg
     3901 tcagccgctc ggcctttgcg gtatccatgg tttgcagcgg aaggcgcacc aactgccaga
     3961 cctgcacgcc ctcccccccg ccgctgcgcc agccgcgaaa gcgggcgcgg tacagcacac
     4021 gggactgctg cggcaggaag taccagcagc cccggctcgg cgtctggcct tgagccagtt
     4081 cgccgcagta gttgtcctgc cgcccgcgca gcaggacgaa tggattgccc gtggcccagc
     4141 cggcgtccag cgggcggccc tgcgccatat tctcggcggc cagcgcatcg aggctgcgcg
     4201 ccaggtgctc tgccgtcgtg ttcatggctt ttgtctcgct gtcgaccgca atatgccaca
     4261 acgagataaa gatcacattg gccagaatca atgtcagcag ccgccaaccg cgtcgctgca
     4321 gcggcgcgac cagcagcagc cgctcgtcgc gcgccggcag cggtttcatt tgacgccctt
     4381 ggccgccgcc gacagctccc acatcggcaa gaacaccccc agcgcgagga tcagcaccat
     4441 cacgcccatg gcgacgatga ggatcggctc gatggcgtcg gccaggcgct tgaggtcata
     4501 gtccacttcc tgttcgtaga agtcggcaac ctcgacgaac aggtcgtcca gcgcgccggt
     4561 ttcctcgccg accgccatca tctgcagcac cagcggggtg aacagaccgc tggcggcggc
     4621 gccacgggtc agcgcctcgc cgcgctcgac gcccgcgcgc atggcggtga tcgcgctgcc
     4681 gatatgccga ttgcccaccg tgtggctgtt gatgcccagg gtctgcagca gcggcaggcc
     4741 agcgcggtac atcatcgcga aggtgcgggt gaaacgggcc agggcaatgc gctcgaaaat
     4801 cggtccgacg atcggcagct tcagcttgta gcgatcccag accagcgcgc cggcatcggt
     4861 ggcgatccag cgcacgaagg cgacgatgct tccagcaatc gccatgccga gcagccacca
     4921 ccagtcctgc atgaattcgg agaaaccgat cagcacccgc gtcggccagg gcagttcggc
     4981 atggaactgg gcgaacacct tggagaaggc cgggatcacc agcaggttga tcaccgccag
     5041 cgccacgctc atggcgatca gcacgaacaa cgggtagcgg gtggcctgct tgatacgctt
     5101 gcgggtctcg cgctccatct ccaggtagcc ggccagctgg cggaaggcct ggtcgagctg
     5161 gccggtgttc tcgccgacgc tgatcatgct gacgaacagc gggttgaaca ccctgggatg
     5221 gttgcccagc gacgcggcca tcgccgtgcc gccttcgagg tcgctgcgca gggtctgcag
     5281 cacctcgcgg aaatacgggt tgcgcgccga ttcggcgagg ccgccgatgg cgcggatgat
     5341 cggtacgccg gccttgttga ggctgtacat ctgccggcag aagatgatca gttcctccag
     5401 ctcgacgcgc tcgcggccaa gcagcttgtt cagctggcgc aggacgtcac cgccaccggc
     5461 ggccttttcc gccagggcgt cgatgctcag cggggtgatg cgctcggcca gcagctcgcc
     5521 ggccagcgcc tcggcactcg ccgcctggcg gctgccgctg atcttgcggc cctgggcatc
     5581 gcgcccgctg aagcggaagt tcgccatcag ccgatccgct cgtcactgag gctggcgctg
     5641 accttgagca cttcctcgac gctggtcaga ccggccaggg caaagtccag cgcactggcg
     5701 gccagtggcc ggtagtacgg ctgggcgcgc gcggcgtcga tgaagccctg ggcatcgtgg
     5761 cggcgcagcg cggcagccag cggcgcatcg atctccagca gctcgtagac gccgatccgc
     5821 cccaggtagc cggtgttgtg gcactggtgg cagccgctgc cacgtacgaa gcgccgaccg
     5881 gccagcggct ggccgctgat gccttccagc cagtggcgct cacgctcctc gggcaggtgc
     5941 tcctcgcggc agtgctcgca gacccggcgc accagacgct gggccagcac ggcgttgagg
     6001 gcgttggcga ccaggaacgg ctcggcgccc atgtcgatca ggcgcagcgg cgaggtcatc
     6061 gcgtcgttgg tgtgcagggt ggacagcacc aggtgaccgg tcagcgcggc gcgcagggcg
     6121 atctcggcgg tctcgcggtc gcggatctcg ccgaccagga cgatgtccgg gtcctggcgc
     6181 agggcggcgc gcagcacgcg ggcgaaggtc agctcgatct tcgggttgac ctgcacctgg
     6241 ctgatccgcg gcaggcggta ttccaccggg tcctcgacag tgatgatctt cttctccggg
     6301 ctgttcagct cggaaaggcc ggcgtacagg gtggtggtct tgcccgagcc ggtcggcccg
     6361 gtgaccagca cgataccgtg cggacgctgc agcagggtgc gaaagcgcgc cagcatgtcg
     6421 gccggcatgc cggtgcggtc gaggctgaac actccggcgc tctggtcgag cagacgcagc
     6481 accaccgact cgccgaactg caccggcatg gtcgacacgc gcacgtcgag gctgtggttc
     6541 ttgacccgga tgttgaagcg gccgtcctgc ggcaggcgct tctcggagat gtccaggccg
     6601 gccatgatct tcaggcgcat gaccagcgcc gaggcgatgc gggtttcctt gatcagttgt
     6661 tcgttgagca cgccgtcgat gcgctggcgc aggcgcagga ccttctcgtc cggctcgatg
     6721 tggatgtccg aggccttcat ctgcaccgca tcctcgaaca gcatctgcag caggcgcacc
     6781 accggggcgt cgctgttgtc gaggctgagg ccgctgaagt cgaagtcgct gtcttccagg
     6841 cgctcgtcga gctggccggc cagctcggcg atctcgccgg tgcgccggta gaagcggtcg
     6901 aggttgtcga gcagatcgct ctcgcgcacc accgccggct tgagcggctg gcgcagcagg
     6961 cggctcatct cgtcgagggc gtagagatcg agcggatcgc ccatgccgac cagcaggccg
     7021 tcgctctcct cgaccagcag gatggcgcgg aaacgccgcg ccagggtctc gggcagacgc
     7081 tggatcagca ccgcgtcgag cttgaactgg cgcaggtcga catagggaat ttccagctgg
     7141 cgcgacaacg cctcgagcag cttctgttcg gagacgaagc cgagatcgac caccgcgcgg
     7201 ccgagcttgg cgccgctgcg cttctgctcc tgcagcgcca gctgcagctg gccctcgctg
     7261 atcagtccgg cctggaccag caggtcaccc aggcgcacct tgcgctgacg cagatcgacg
     7321 ctatccatca ggacgctcct ccgagggcct gcgcccgctc gcgggcatag cggcgactgc
     7381 cgtcgtccag gccctggccc tgcaaggcct ggcgatagtg ctgggcggcc gcggcgcgct
     7441 cgccgagctg ctcgagagcg atgcccaggc ccagctgcca ggcggactgc tcagggcgca
     7501 gcagcaccag gttgcggtac agctgggcgc tctcgcgcca ctggccggtc tgctggtagc
     7561 tggcggcgag cagcgcgtgg taggccggat cgccggccag cgggggcaga tgggcggcca
     7621 gggtcgccac
//
LOCUS       DATCUN010000129         7842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_5263, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000129 DATCUN010000000
VERSION     DATCUN010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7842
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5263"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(428..1627)
                     /gene="tyrS"
                     /locus_tag="VIO83_05170"
     CDS             complement(428..1627)
                     /gene="tyrS"
                     /locus_tag="VIO83_05170"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY8330701.1"
                     /translation="MKSVEEQLALIKRGAEEILVESELVAKLQRGQPLRIKAGFDPTA
                     PDLHLGHTVLINKLRQFQELGHQVIFLIGDFTGMIGDPSGKNATRPPLTRDQVLENAE
                     TYKAQVFKILDPAKTEVAFNSTWMDQLSPADFIRLASQYTVARMLERDDFHKRYTSNQ
                     SIAIHEFLYPLVQGYDSVALRADVELGGTDQKFNLLMGRELQRAYGQESQVILTMPLL
                     EGLDGVKKMSKSLGNYIGIQEAPGVMYGKLVSIPDALMWRYFELLSFRPMAEIDELRR
                     DVERGANPRDIKIQLAEEIVARFHGEEAAAGAHRSAGNRLKDGELPEDLPEVGLVSAE
                     PMSIAAVLNKAGLVKNAATARDMLNAGSVKVDGVVVDRDFVFALGKTHVCQAGKKAFA
                     RVTVCLE"
     gene            1796..3175
                     /locus_tag="VIO83_05175"
     CDS             1796..3175
                     /locus_tag="VIO83_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8330702.1"
                     /translation="MTDKSRNTPLYPKSHLLAASGIAALLSLTLLVFPSSQVEAKKTL
                     LPLEIENSSDVLADKDDLQPSLVTSERLAVEKPLETLPPETQAAKPAQPRETHITVAN
                     GDTLSSVFAKAGLPDETLHDLLASNRDARQLTRIKSGQIFHITRNDQGGLESLNSRLS
                     SLESVQVTRTEQGFSVKRDLIKPATRQAYARGVIDSSLFVATKRAGLPHSQAMELASI
                     FGYDIDFALDLRDGDEFEVIYEEKVVDGKKVGGGAILAARFTNRGKTFSAVRYTDRQG
                     SSTYYRADGSSMRKAFIRTPIDFARISSRFTQARRHPVLNKVRAHKGVDYAAPTGTPI
                     KAAGDGRVQLAGRKGGYGNTVVIQHGQRYRTLYGHMHRFAKGIRNGSQVKQGQIIGYV
                     GTSGLSTGPHLHYEFQVNGVHVDPLSQKLPMADPIAGAERKRFLQQSQPLLARMDQER
                     SSLLALNKR"
     gene            3179..4267
                     /locus_tag="VIO83_05180"
     CDS             3179..4267
                     /locus_tag="VIO83_05180"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEY8330703.1"
                     /translation="MPLYIGVMSGTSLDGLDVALIEQEQAPRLLASHYTPMPADLGRQ
                     LLALCSSGDDEIARSALAEQQWAELAAQAIQQLLQRERLDPQQIRAIGSHGQTVRHEP
                     QRGFTVQIGNPALLAELTGICVVADFRRRDVAAGGQGAPLVPAFHQALFGCERQRAIL
                     NVGGFSNLTLLAPQQPVRGFDCGPGNVLLDAWIRRHHGEAFDRDGAWAASGIADPQLL
                     QDLLADDYFSLHGPKSTGREHFNLDWLLAQLQARAPIAAADVQATLLELTARSIATAL
                     HAALPAAEELLVCGGGAHNKRLMQRLAELLPTCTVESTDTRGLPADWIEAMAFAWLAH
                     CCLEGIPANCPEVTGAAGPRVLGAIYPA"
     gene            complement(4325..4675)
                     /gene="erpA"
                     /locus_tag="VIO83_05185"
     CDS             complement(4325..4675)
                     /gene="erpA"
                     /locus_tag="VIO83_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY8330704.1"
                     /translation="MSIETFTPTSLQFTQGAANKVKSLIDEEGNPRLKLRVFVTGGGC
                     SGFQYGFTFDEEQADDDTVVERDGVRLVVDPMSFQYLVGAEVDYQEGLEGSRFVIKNP
                     NASTTCGCGQSFSI"
     gene            complement(4849..5577)
                     /locus_tag="VIO83_05190"
     CDS             complement(4849..5577)
                     /locus_tag="VIO83_05190"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HEY8330705.1"
                     /translation="MPPLAGAIAGAVPQRQHGRQLVLAVLLLALPAAFFAGYWLSERK
                     AGELLNQARLQDEQLKLRTAELDQLRRRLALRDSGERLAVQVGEQNRQTIKLLEEQIF
                     QLQQELATYKGVLDPAASREGLQIRSFELQATADPRRFRYQILLSRVGSDEQALEGTL
                     QIELRGRRDGKATSLRLAELAEGVPDDGLSFAFRHFQAIPEGGRFAELALPAGFEPTD
                     VRLRASVTGQRQPLERSFRWAELQ"
     gene            complement(5577..6611)
                     /gene="argC"
                     /locus_tag="VIO83_05195"
     CDS             complement(5577..6611)
                     /gene="argC"
                     /locus_tag="VIO83_05195"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY8330706.1"
                     /translation="MIKVGIVGGTGYTGVELLRLLAQHPQAQVEVITSRSEEGVRVVD
                     MYPNLRGHYDELRFSMPDVARLGACDVVFFATPHGVAHALAGELLATGTRVIDLSADF
                     RLQDAEEWAKWYGQPHGAPDLLPEAVYGLPEVNREAIKGARLIAVPGCYPTATQLGLI
                     PLLEAGLADPASLIADCKSGVSGAGRGASVGSLLCEAGESMKAYAVKGHRHLPEISQG
                     LRRAAGGAVGLTFVPHLTPMIRGIHATLYARVADRSVDLQALFEQRYAGEPFVDVMPA
                     GSHPETRSVRGANVCRIAVHRPQGGDLVVVLSVIDNLVKGASGQAVQNMNILFGLDER
                     MGLAHAALLP"
     gene            6754..7182
                     /gene="hemJ"
                     /locus_tag="VIO83_05200"
     CDS             6754..7182
                     /gene="hemJ"
                     /locus_tag="VIO83_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HEY8330707.1"
                     /translation="MLYLWLKALHIIAIVCWFAGLFYLPRLFVYHAMSEDTPSHERFC
                     IMERKLYRGIMIPSMLASLVFGIWMLVANPTLFGSGAWLHAKLTLVALLIGYHHWCGA
                     LLKRFARGDNQRSHVFYRWFNEVPVLFLLAIVILVVVKPF"
     gene            7272..>7842
                     /locus_tag="VIO83_05205"
     CDS             7272..>7842
                     /locus_tag="VIO83_05205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8330708.1"
                     /translation="MPQYALITGASSGIGLALAEALARRGHALILVARERPALESIAC
                     ELSQRFGVEVMFRVCDLTEPLQLSGLLYEVEQCDYQIELLINNAGTACAGSFLDNDWQ
                     RERKLLELNVFALARLCHALGNAMARRGSGRILNVASLAAFQPGPWLSSYYASKAFVL
                     HFSEGLREELQGQGVQVPLAPGCTLVHGLR"
BASE COUNT         1469 a         2743 c         2314 g         1316 t
ORIGIN      
        1 aatcttgcga ctgtcagtct tatctcacaa gcacccacac gaattgcttg attcaacttg
       61 ttaaagagcg tttcgaccga gccagtgttt cgtctcaacc gaggccgcga attctacagc
      121 agccttacaa cctgtcaagc gtttcgtgaa aatttctttt cgtttcaacc acttgcgcta
      181 gcttcaaccc ggaggccgcc gcagcgagga ggtgaataat acacccctga aaaccgatgt
      241 caacaccccg ctgcactttc cggcaatcac cgaaagcgct ggcgagacct tgatctcgcc
      301 agcgcctgcc actcagccac cggacgcaaa ccattgatga tcaagaactt ctgcccagct
      361 tgtcgctgga ggcgggcaca cctatacgga tgccggcacc gctatcaacg acgcctttcc
      421 aggcaaatca ctccaggcac acagtaaccc gggcaaaagc ctttttgccc gcctggcaga
      481 catgggtctt gccaagcgca aagacgaagt cgcgatccac cacaacacca tccaccttta
      541 cactgccggc attcagcatg tcgcgggcag tagcggcatt cttgaccaat cccgccttat
      601 taagtacggc cgcgatcgac ataggctccg cggacaccaa tcccacctcc ggcagatcct
      661 caggcagctc gccatccttc agccgattgc ccgccgaacg atgcgcccca gcggcagcct
      721 cttcaccgtg gaagcgcgcc acaatctcct cggccaactg gatcttgatg tcccgcggat
      781 tggcgccacg ctcgacgtcg cgacgcaact cgtcaatctc cgccatgggc cggaagctca
      841 gcaactcgaa atagcgccac atcagcgcat caggaatgga caccagcttg ccgtacatga
      901 cacccggcgc ctcctggatg ccaatatagt tgccgagcga cttggacatc ttcttgacgc
      961 catccagccc ttcgagcaac ggcatggtca ggatgacctg cgactcctgg ccataggcgc
     1021 gctgcagttc gcgccccatg agcagattga acttctggtc ggtgccgccc agctccacat
     1081 cggcccttaa ggccactgag tcatacccct gcaccagcgg ataaagaaac tcatggatgg
     1141 cgatcgactg gttgctggtg tagcgcttgt ggaagtcgtc gcgctcgagc atccgcgcca
     1201 cggtgtactg cgacgccagg cgaatgaagt cggccggcga cagctgatcc atccaagtcg
     1261 aattgaaggc cacctcggtc ttggccggat cgaggatctt gaatacctga gccttgtagg
     1321 tctcggcatt ctccagaacc tggtcgcgcg tcagcggcgg acgcgtggcg ttcttgccgc
     1381 tcggatcacc gatcatcccg gtgaagtcac caatcaggaa gatgacctga tgccccaact
     1441 cctggaactg acgcaacttg ttgatcagca cggtgtgccc gagatggagg tccggggcgg
     1501 tggggtcgaa accggccttg atccgcaatg gctggccgcg ctgcagcttg gccaccagtt
     1561 ccgactcgac aagaatttcc tctgcgccgc gcttgataag cgccagctgc tcttcgaccg
     1621 acttcatgaa gctgcccata gcgaaaaagg gctcacgata caagatcgcg caagatctgc
     1681 aaaatctgcg cagcagccca cagccgcgca tcgcaaagcg ggcgcttcgg gttgcctcaa
     1741 agaggttttg attataattt agacggttat tgcactccaa agaaccctac ccgctatgac
     1801 ggataagtcc aggaacacac ccctctaccc caaaagccac ttgctggctg cgagcgggat
     1861 tgcggcgttg ctgagcctga ctctgctggt attccccagc agccaggtag aagccaagaa
     1921 gaccctgctg ccactcgaga tcgaaaacag cagcgacgtg cttgcagaca aagacgacct
     1981 ccagcccagc ttggtcacgt ccgagcgtct tgctgtcgaa aagccgctcg aaacgctgcc
     2041 ccccgaaacg caggccgcca aaccggcaca gccacgcgaa acccatatta cagttgccaa
     2101 tggcgacacc ctgtccagcg ttttcgccaa ggccggcctg cctgacgaaa ccctgcacga
     2161 tctgctggcc agcaaccgcg acgcccggca gctgacccgc atcaagtccg gacagatctt
     2221 ccacatcacg cggaacgatc aaggcgggct cgagtccctg aactcgcggc tgagctccct
     2281 ggaaagcgtg caggtcaccc ggaccgaaca aggcttcagc gtcaagcgcg acctgatcaa
     2341 gccggctact cgccaggcct atgcccgcgg cgtcatcgac agctcgctgt tcgtcgccac
     2401 caagcgcgcc ggcctgccgc acagtcaggc catggagctg gccagcatct tcggctacga
     2461 catcgacttc gctctcgacc tgcgcgacgg cgatgaattc gaagtgatct acgaagaaaa
     2521 agtggttgac ggcaaaaagg tcggtggcgg cgccattctt gccgcgcgct tcaccaaccg
     2581 cggcaagacc ttcagcgccg tgcgctacac cgaccgccag ggcagcagca cctactacag
     2641 ggccgatggc agcagcatgc gcaaagcctt catccgcacg ccgatcgatt ttgcccgcat
     2701 cagctcgcgc tttacccagg cccgccgcca tccggtgttg aacaaggtac gcgcgcacaa
     2761 gggcgtcgac tatgctgcgc cgaccggtac gccgatcaag gccgccggcg acggcagggt
     2821 gcaactcgcc ggccgcaagg gcggctatgg caataccgtg gtgatccagc acggccagcg
     2881 ctatcgcacc ctgtatgggc acatgcaccg cttcgccaag ggcatccgca acggcagcca
     2941 ggtcaagcag ggccagatca tcggctacgt cggcaccagt ggtctctcca ccggtccgca
     3001 cctgcattac gagttccagg tcaacggcgt gcatgtcgac ccgttgagcc agaagctgcc
     3061 gatggccgac cccatcgccg gtgccgagcg caagcgcttc ctccagcaga gccagcccct
     3121 gctggcacgc atggatcagg agcgctcttc gctgctggcc ctgaacaagc gctaagccat
     3181 gccgctctat atcggggtga tgtccggcac cagcctggac ggactggacg tcgccttgat
     3241 cgagcaggag caggcacctc gcctgcttgc ctcccattac acgcccatgc cggccgacct
     3301 cggccggcag ctgctggccc tctgtagcag cggtgacgac gagatcgccc gcagcgcctt
     3361 ggccgagcag caatgggccg agcttgcggc tcaggccatc cagcaactgc tgcagcgcga
     3421 gcgacttgat ccgcagcaga tccgtgccat cggcagccac ggccagaccg tgcgccatga
     3481 gccgcagcgc ggcttcaccg ttcagatcgg caatccggcc ctgctcgccg agctcaccgg
     3541 catctgcgtg gtcgccgact tccgccgccg cgatgtcgcg gccggcggcc agggcgctcc
     3601 gctggtcccg gcgttccacc aggccctgtt cggctgcgaa cggcagcggg ccatcctcaa
     3661 tgtcggcggc ttcagcaatc tgaccctgct ggccccgcaa cagccggtgc gcggcttcga
     3721 ctgcgggccg ggcaatgtgc tgctggatgc ctggatccgg cgccatcatg gcgaggcctt
     3781 cgaccgcgac ggcgcctggg cggccagtgg catagcggat ccgcagctgc tgcaggacct
     3841 gctggccgac gactacttca gcctgcatgg gccgaaaagc accggccgcg agcacttcaa
     3901 tctggactgg ctgctggcgc agctgcaggc acgggcgccg atcgctgctg cagacgtgca
     3961 agccaccctg ctggagctga cggcccgcag catcgccacg gctctccacg ccgccctgcc
     4021 ggctgcggaa gaactgctgg tctgcggcgg tggagcgcac aacaagcgcc tgatgcagcg
     4081 cctcgccgaa ctgctgccaa cctgtaccgt ggagagcacc gacacccgcg gcctgccggc
     4141 cgactggatc gaggccatgg cctttgcctg gctggcgcac tgctgcctgg aaggtatccc
     4201 ggccaactgc cccgaagtca caggcgccgc tgggccgcgc gtcctcggcg cgatctaccc
     4261 ggcctgagcg tcacgccaac aaaaacgccg cgcacaagcg cggcgttttt ccattatcag
     4321 cgactcagat cgagaacgac tgtccgcaac cacaagtggt gctggcgtta ggattcttga
     4381 tcacgaagcg tgagccttcg agaccttcct ggtaatcgac ctcggcgccg accaggtact
     4441 ggaagctcat cggatcgacc accaggcgca caccatcgcg ctcgaccacg gtatcgtcat
     4501 cggcctgctc ttcatcgaag gtgaagccgt actggaagcc cgagcagccg ccgccagtga
     4561 cgaatacgcg cagcttgagg cggggattgc cctcctcgtc gatcagactc ttcaccttgt
     4621 tggccgctcc ctgagtgaac tgcagcgagg tgggggtgaa ggtttcaatg ctcatatcca
     4681 gaactccagg cgcacagccg tcttgcagca tagaagcgag gcattatcgc tcaatcgccg
     4741 accaagcgaa acagtcaact actctgccgc ggttgctgcg tagcaaaatg atggccgcag
     4801 ccaaggctgc ggatggcttt tcggcggtct ctccgccccg agggtcgctc attgcagctc
     4861 ggcccagcga aagctgcgct ccagcggctg acgctggccc gtcacactgg cccgcaggcg
     4921 cacgtcagtc ggctcgaaac ctgccggcaa tgccaactcg gcaaaacgcc caccttcggg
     4981 aatcgcctgg aaatgccgga aggcgaagga caggccgtcg tcaggcacgc cctctgccag
     5041 ctcggccagc cgcaggctgg tcgccttgcc atcgcgccgc ccacgcagct cgatctgcag
     5101 cgtgccctcc agggcctgct cgtccgagcc gacccggctg agcaggatct gataacggaa
     5161 gcggcgcgga tcggcggtgg cctgcagctc gaacgaacgg atctgcagac cttcgcgact
     5221 ggcagcggga tccagcaccc ccttgtaggt cgccaactcc tgctgcaact ggaagatctg
     5281 ctcctccagc agcttgatgg tctgccggtt ctgctcgccg acctgcactg ccaagcgctc
     5341 gccgctgtcc cgcagcgcca gccgccgccg caactggtcc agctcggccg tgcgcagctt
     5401 cagctgctca tcctgcagac gggcctgatt gagcaactcg ccggccttgc gctcgctcag
     5461 ccagtagccg gcgaagaagg ccgccggcag cgccagcaac aacaccgcca gcaccaactg
     5521 gcgaccgtgc tggcgctgcg ggacagcccc ggcgatcgcg ccggcgagag gcggcatcag
     5581 ggcagcagcg ccgcgtgtgc cagccccatc cgctcatcca ggccgaacag gatgttcatg
     5641 ttctgcacgg cctgcccgga ggcgcccttg accaggttgt cgatcaccga cagcaccacc
     5701 accaggtcgc cgccctgcgg ccggtgcacg gcgatgcggc agacgttggc gccgcgcacg
     5761 ctgcgggttt ccggatggct gccggccggc atcacgtcga cgaacggctc gccggcatag
     5821 cgctgctcga acaacgcctg caggtcgaca ctgcgatcgg ccacgcgggc gtacaaggtg
     5881 gcgtgaatgc cacggatcat cggggtcagg tgcggcacga aggtcaggcc gaccgctccg
     5941 ccggcggcgc ggcgcaggcc ctggctgatt tccggcaggt ggcgatggcc cttgaccgca
     6001 taggccttca tgctttcgcc cgcctcgcac agcagcgaac cgacgctggc gccgcggccg
     6061 gcaccgctga cgcccgactt gcagtcggcg atcaggctcg ccggatcggc cagaccggcc
     6121 tccagcagcg ggatcaggcc gagctgggtg gcggtcgggt agcagcccgg cacggcgatc
     6181 agccgtgcgc ccttgatcgc ctcgcggttg acctccggca ggccatacac ggcctccggc
     6241 agcaggtcag gcgcgccgtg cggctggcca taccacttgg cccactcctc ggcatcctgc
     6301 aggcggaagt cggcggacag gtcgatcacc cgggtgccgg tggccagcag ctcgccggcc
     6361 agggcatgcg ccacgccgtg cggggtggcg aagaacacca catcgcaggc gcccagacgc
     6421 gccacgtccg gcatgctgaa gcgcagctcg tcatagtgcc cgcgcaggtt ggggtacatg
     6481 tcgacgaccc gcacaccctc ctccgagcgc gaggtgatca cctcgacctg tgcctgcgga
     6541 tgctgggcca acagacgcag caactccacc ccggtatacc cggtcccccc tacaatgccg
     6601 accttgatca tctcgtgtcc ccgcgggcca tctgtgaaaa acaatagtgc gccatgataa
     6661 ggccgcggcc cggccggagc cagccgcggg atgacgccgt gcccggcctg cgactaccat
     6721 ctgcgcagac tctaatcgag aaggacgtcc cgcatgctct acctgtggct caaggccctg
     6781 cacatcatcg ccatcgtctg ctggttcgcc ggcctgttct atctgccgcg cctgttcgtc
     6841 taccacgcga tgagcgagga cacgcccagc cacgagcgct tctgcatcat ggaacgcaag
     6901 ctttaccgcg gcatcatgat tccctcgatg ctcgccagtc tggtgttcgg catctggatg
     6961 ctggtcgcca accccaccct gttcggttcc ggcgcctggc tgcacgccaa actgaccctg
     7021 gtggccctgc tgatcggcta ccaccactgg tgcggcgccc tgctcaagcg cttcgcccgc
     7081 ggcgacaacc agcgcagcca cgtgttctat cgctggttca acgaggtgcc ggtgctgttc
     7141 ctgctggcca tcgtcatcct ggtggtggtc aagccgttct gaagcgctga atcgggcagc
     7201 cagcgccggc cgagcggagt acactgcgcg ctttttgcgc ggccccgccc ggcacgtcat
     7261 ggagaccccg catgccccaa tatgccctga tcaccggcgc ttccagcggc atcggcctgg
     7321 ccctcgccga agccctggcg cggcgcggtc acgcactgat cctggtcgcc cgcgaacgcc
     7381 cggcgctgga aagcatcgcc tgcgagctga gccagcgctt cggcgtcgaa gtgatgttcc
     7441 gcgtctgcga cctgaccgag ccgctgcaac tgtccggcct gctctatgaa gtggagcagt
     7501 gcgactacca gatcgaactg ctgatcaaca acgccggcac cgcctgcgcc ggcagcttcc
     7561 tcgacaacga ctggcagcgc gaacgcaagc tgctggagct caacgtgttc gccctggcgc
     7621 gcctgtgcca cgccctcggc aacgccatgg ctcgccgcgg cagcggacgc atcctcaatg
     7681 tcgcctcgct ggccgccttc cagcccgggc catggctgag cagctactac gccagcaagg
     7741 ccttcgtcct gcatttctcc gaaggcctgc gcgaagaact gcagggacag ggcgtgcagg
     7801 tcccacttgc cccaggttgc accctggttc atggtcttcg tt
//
LOCUS       DATCUN010000130         3096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_268499, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000130 DATCUN010000000
VERSION     DATCUN010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268499"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VIO83_05210"
     CDS             complement(<1..407)
                     /locus_tag="VIO83_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330709.1"
                     /translation="MERFSRRCLHHLPLLLTLLLAAAALLYGPIAQLENYHAFADQLT
                     LLGIPHALDVLSNLGFALVALWGLWRLLPARRSAELVAGWPGYALFLGGLLLTALGSS
                     WYHLAPDDHRLVWDRLPIALASAGLLAGVWGDCR"
     gene            445..1260
                     /locus_tag="VIO83_05215"
     CDS             445..1260
                     /locus_tag="VIO83_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8330710.1"
                     /translation="MVAPRETLAEKGARRTLKGIEKRYFPLAMTELDQLLTTLKRQLK
                     ARGMTYRDLAAALDLSEASVKRLFASGSFTLERLLQISHLLGFTLAELSQEAALGASR
                     LHTLSESQERELVSDAKLLLVAVCVLNQWTLADMLSVYQLSEAECIQRLARLDRLGLL
                     QLLPGNRVRLNVARDFDWLPDGPIRRFFLRQGLGEFLNSEFAGSEQSLAFAHGMLTAA
                     ALAKLQLEVRRLRQKFAELHEESLAAPLAKRHGTGLLLALREWELGAFTALRR"
     gene            complement(1359..1925)
                     /locus_tag="VIO83_05220"
     CDS             complement(1359..1925)
                     /locus_tag="VIO83_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330711.1"
                     /translation="MLLQTYEAQNLLCNLSVKGATSQADTYAFRGDLVLEEGEIADDA
                     GRRLPPTAVVKQAALLVKGNKLTMVSGIITELAQLPLFIERYRHDLAADVHVLFYVEN
                     LGKALSTDLDGVGIILLPHADGGAAWNTLMGDLRLEKDDFKGQSAEDKVVTMADALAT
                     YKPKLDSLGFNECLTFVSAARREHRGPV"
     gene            complement(1962..2825)
                     /locus_tag="VIO83_05225"
     CDS             complement(1962..2825)
                     /locus_tag="VIO83_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEY8330712.1"
                     /translation="MNSSLMMKEILAQLGAGEIVPYLGPGVLRGVVDRQTGKAMPADS
                     DSLILAMTGGQPMAPRLMYEFPRAAMHLENKKGRSFIERFLTETYGGDQWTPSPVHQW
                     LADQRLPYVIDCNRDTQLQRCYADRAHTLVVGAARIAATPYRFDLYQGDAGQYRRIEL
                     AEVDGALPVLFKPLGTPLPKPGYVASDADFVDYITELMGGFAVPAWVKLKRRNKRYLF
                     LGMHFNRDTERMVMSDLIHDAATPAGWALIAEPSDKERKVCARKDLQLIEADWSSLFA
                     LANPAAAERVA"
BASE COUNT          513 a          971 c         1102 g          510 t
ORIGIN      
        1 cggcagtcgc cccacacccc ggcgagcagg ccggcggagg cgagggcgat gggcaggcgg
       61 tcccagacca ggcgatggtc gtcgggggcc aggtggtacc agctcgagcc gagcgcggtc
      121 agcagcaggc cgccgaggaa cagcgcgtag ccaggccagc cggccaccag ctcggccgaa
      181 cggcgggcgg gcagcagacg ccacaggccc cacaaggcga ccagcgcgaa accgaggttg
      241 gacagcacat ccagcgcgtg ggggatgccc agcagggtca gctggtcggc gaaggcgtgg
      301 tagttctcca gctgggcgat ggggccgtag agcagggcgg cggcggccag cagcagggtg
      361 agcagcaggg gcaggtggtg caggcagcgg cgagagaagc gttccatggc ggtctccacg
      421 aggtaggtgg cctcaggatg gacgttggtt gcgcctcgcg aaaccctggc ggaaaaaggc
      481 gcgcggcgta cactgaaagg tatcgaaaaa cgctactttc ccttggccat gactgaactc
      541 gaccagctgc tgaccaccct caagcgccag ctcaaggccc gcggcatgac ctatcgcgat
      601 ctggccgccg ccctcgatct ctccgaagcc agcgtcaagc gcctgttcgc cagcggcagc
      661 ttcaccctcg agcggctgct gcagatcagc cacctgctcg gcttcaccct cgccgagctg
      721 agccaggagg cggcactcgg cgcaagccgc ctgcacaccc tcagcgaaag ccaggagcgc
      781 gagctggtgt ccgacgccaa gctgctgctg gtggcggtgt gcgtgctcaa ccagtggacc
      841 ctggccgaca tgctcagtgt gtaccagctc agcgaggccg agtgcatcca gcgcctggcc
      901 cggctcgacc gcctgggcct gctgcagctg ctgccgggca accgcgtgcg cctcaacgtg
      961 gcgcgcgact tcgactggct gcccgacggg ccgatccgcc gcttcttcct gcgccagggc
     1021 ctcggcgagt tcctcaacag cgagttcgcc ggcagcgagc agagcctggc cttcgcccac
     1081 ggcatgctga ccgcggcggc gctggcgaaa ctgcagctgg aggtgcgccg gctacggcag
     1141 aaattcgccg agctgcacga ggagagtctg gccgcgccgc tggccaagcg ccatggcacc
     1201 ggcctgctgc tggcgctgcg cgagtgggag ctgggcgcgt tcaccgcgct gcgccggtag
     1261 gaggcagcaa gctggctcct gtggggcgcg aaaaaaaact cccccaggag cgggggagtg
     1321 gcttattggc ctagctggac agcaagggaa cggggaactc agaccggacc gcggtgctcg
     1381 cggcgcgcgg cgctgacgaa ggtgaggcat tcgttgaagc cgaggctgtc gagcttcggc
     1441 ttgtaggtgg ccagggcgtc ggccatggtg acgaccttgt cttcggcgct ctggcccttg
     1501 aagtcgtcct tttccaggcg caggtcgccc atcagggtgt tccaggccgc gccgccgtcg
     1561 gcgtggggca gcaggatgat gccgacgcca tcgaggtcgg tgctcagcgc cttgcccagg
     1621 ttttccacat agaacagcac gtgcacgtcg gcggccaggt cgtggcgata gcgctcgatg
     1681 aacagcggca gctgggccag ctcggtgatg atgccgctga ccatggtcag cttgttgccc
     1741 ttgaccagca gcgcggcctg tttgaccacg gcggtcggcg gcaggcggcg gccggcgtcg
     1801 tcggcaatct cgccttcctc caggaccagg tcgccgcgga aggcgtaggt gtcagcctgg
     1861 ctggtggcac ctttgacgct gaggttgcac agcaggttct gcgcttcgta ggtttgcagc
     1921 agcatggtgg ggtctctctg tgtcggggga agaaaggggg atcaggccac ccgctcggcg
     1981 gcggcggggt tggcgagggc gaacagactg ctccagtcgg cctcgatcag ttgcaggtcc
     2041 ttgcgcgcgc agaccttgcg ctccttgtcg ctcggctcgg cgatcagcgc ccagccggcc
     2101 ggcgtggcgg cgtcgtggat caggtcgctc atcaccatgc gttcggtgtc gcggttgaag
     2161 tgcatgccga ggaacaggta gcgcttgttg cggcgcttga gcttgaccca ggcgggcacg
     2221 gcgaagccgc ccatcagctc ggtgatgtag tcgacgaagt cggcatccga ggccacatag
     2281 ccgggcttgg gcagcggcgt gccgagcggc ttgaacagca ccggcagtgc gccgtccacc
     2341 tcggccagct cgatgcgccg gtactggccg gcgtcgccct ggtagaggtc gaagcggtaa
     2401 ggcgtggcgg cgatgcgcgc ggcaccgacc accagggtgt gcgcgcggtc ggcatagcag
     2461 cgctgcagct gggtgtcgcg gttgcagtcg attacgtagg gcaggcgctg gtcggccagc
     2521 cactggtgca cgggcgaggg cgtccactgg tcgccgccgt aggtctcggt caggaagcgc
     2581 tcgatgaagc tgcgcccctt cttgttctcc aggtgcatgg ccgcgcgcgg gaattcgtac
     2641 atcagtcgcg gcgccatcgg ctggccgccg gtcatggcga ggatcaggct gtcgctgtcg
     2701 gccggcatgg ccttgccggt ctggcgatcg accaccccgc gcagcacgcc gggacccagg
     2761 tagggaacga tctcgcccgc gccgagctgc gcgaggattt ccttcatcat caagctgctg
     2821 ttcaccgggg ctctcctgca gggatagagg ggcgcaataa gtgcgcaata acgctgcagt
     2881 ccttcgcaat atgcgcgcca ctttccggtg attcagacaa gttgttgata tgcgaaggat
     2941 ttattcctgc ttcagcaatt aagccagcaa tcaagtcgca acaagtccgg acttttcacg
     3001 acaaattgtc atctatgtca caaacgcaaa gtggccggcc aagtgccgcc ggataattgg
     3061 ccggagtttg ttgggttgtc gtcattgcca cacgtt
//
LOCUS       DATCUN010000131        12077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_99614, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000131 DATCUN010000000
VERSION     DATCUN010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 12077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12077
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99614"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /gene="xdhC"
                     /locus_tag="VIO83_05230"
     CDS             <1..498
                     /gene="xdhC"
                     /locus_tag="VIO83_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HEY8330713.1"
                     /translation="EPLARPRVEVAVFGAGHVGRALVPLLAGLPCRVRWIDSRVAEFP
                     AQLPPGVERVVSEEPVDEVDELPPGSYCIVMTHDHQLDLELAERILRRGDFVYFGLIG
                     SRTKRARFEHRLRERGLAAESIARMRCPLGLPEVRGKLPLEIAVSVAGEVIATYSAER
                     DRSEP"
     gene            596..1063
                     /locus_tag="VIO83_05235"
     CDS             596..1063
                     /locus_tag="VIO83_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014717486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY8330714.1"
                     /translation="MANKRYSCIGLSNPKSPENVGSIMRAAGCYEVSSVFYTGTRYDR
                     ARDFVTDTKKVHQDIPLINIDDLRKILPFGCTPVAVELVEGARSLPEYTHPDRALYIF
                     GPEDGSLDKQIRDWCEDVIYIPTNGCMNLAATVNVVLYDRLAKGNNTRSGPLF"
     gene            1237..2556
                     /locus_tag="VIO83_05240"
     CDS             1237..2556
                     /locus_tag="VIO83_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8330715.1"
                     /translation="MSAEHSSTAPVARPLTRSDFKTLSLSALGGALEFYDFIIFVFFA
                     AVVGKLFFPAEMPEWLRQLQTFGIFAAGYLARPLGGIVMAHFGDLLGRKRMFTLSIFM
                     MAVPTLCMGLLPTYAQIGLWAPLALLALRVVQGAAIGGEVPGAWVFVAEHVPARRIGF
                     ACSTLTAGLTAGILLGSLTASAINRAFSAEELADYAWRIPFLLGGVFGLVSVYLRRWL
                     HETPVFAELQLRQSLAEELPLKAVMREHRPAVLLSMLLTWVLSAGIVVVILMTPTILQ
                     TLHGFTPGEALQANSLAIVALSLGCVLAGLAADRFGAGPTFVFGGLLLLLSSALFYAG
                     IASHRDLLIPLYTLAGLGVGTIGAIPMVMVKAFPVAVRFSGVSFSYNLAYAICGGMTP
                     ILVSLLLKWSPLGPGYYVGALSLLFILAGVGLWRRAASAMAPAGCAP"
     gene            complement(2641..5250)
                     /gene="acnB"
                     /locus_tag="VIO83_05245"
     CDS             complement(2641..5250)
                     /gene="acnB"
                     /locus_tag="VIO83_05245"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEY8330716.1"
                     /translation="MLEAYRKHVEERAAQGVVPQPLNAEQTAGLVELLKNPPAGEEEF
                     LVDLITNRVPAGVDEAAYVKAGFLSAIAKGETASPLISKQRAVELLGTMQGGYNIATL
                     VELLDNAELAEVAAAQLKHTLLMFDAFHDVSERAKKGNAAAKAVLQSWADAEWFVNRP
                     ALAEKITLAVFKVPGETNTDDLSPAPDAWSRPDIPLHALAMLKMARDGINPDVPGSVG
                     PLKQMEELKAKGFPVAYVGDVVGTGSSRKSATNSVLWFFGDDIPNVPNKRAGGFCFGT
                     KIAPIFYNTMEDAGALPIEFDCTNLAMGDVIDVYPYKGEVRRHDSDELVTTFQLKTDV
                     LLDEVRAGGRIPLIIGRGLTEKARAELGLAPSTLFKKPVAPADSGKGFSLAQKMVGRA
                     CGLPEGQGVRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAA
                     YPKPIDVVTHHTLPDFIMNRSGVSLRPGDGIIHSWLNRMLLPDTVGTGGDSHTRFPIG
                     ISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKLQPGITLRDLVHAIPYYAIQAGLL
                     TVEKKGKKNIFSGRILEIEGLDGLTVEQAFELSDASAERSAAGCAIKLPEEAIAEYLR
                     SNITMLRWMIGEGYGDARTLERRAQAMEAWLANPKLLEADKDAEYAAVIEIDLADVKE
                     PVLCAPNDPDDARLLSTVAGQKIDEVFIGSCMTNIGHFRAAGKLLDKVKGGIPTRLWL
                     APPTKMDAHQLTEEGYYGIYGRAGARMEMPGCSLCMGNQARVQTGSTVVSTSTRNFPN
                     RLGDATNVFLASAELAAVASIIGKLPTVEEYMGYAKNIDSMAADVYRYLSFDQMAEYR
                     DAAAKANIPVIQA"
     gene            5577..6053
                     /locus_tag="VIO83_05250"
     CDS             5577..6053
                     /locus_tag="VIO83_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEY8330717.1"
                     /translation="MPHQRIKTPCIGLCSTVYGDLVCRGCKRFHHEVVNWNLYGEDEK
                     RAVWQRLETLLVQVVAAKLEVFDPHLLRHQLQQRQIRFVERQSPYCWAYQLIARGARM
                     IQQLDAYGIVLLPEFRDSSLPELRDAIDREYFLLSEAHYQRYIAPLFLVEGREGRG"
     gene            complement(6060..6923)
                     /locus_tag="VIO83_05255"
     CDS             complement(6060..6923)
                     /locus_tag="VIO83_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003161622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8330718.1"
                     /translation="MQAIRSILVVLEPNQPEGLALKRATLIANATQSELHLLVCDTQR
                     NHDVFLDDLALSLREEGHTVSTRQDWQETTYQTIIAAQQAEGCGLVIKQHYPDNPLKK
                     ALLTPDDWKLLRYCPCPVLLVKTDRPWTGGKVLAAVDVGNDEPEHRSLHANIVCHAHD
                     IAHLAKGELHVLSAHPAPLLTAADPSFQLSETIAARYREACQSFQDEYGLGSHQLHIA
                     EGPADLLIPQMALQLDAAVTVIGSVARSGLSGALIGNTAEVVLDTLQSDVLVLKPDDI
                     CDQLEKLTATE"
     gene            7065..7667
                     /gene="miaE"
                     /locus_tag="VIO83_05260"
     CDS             7065..7667
                     /gene="miaE"
                     /locus_tag="VIO83_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HEY8330719.1"
                     /translation="MSLLPEIESFLLCPTPQAWIDQALQHPEILLIDHANCEKKAAST
                     ALTLLYRYVEKEDLQYKLSRLAREELRHFEQVAALMKKRGVAYRPVSASLYAQRLHAH
                     IRKSEPGKLVDTLIVGAYIEARSCERFYRLAPFLDEELGKFYLSLLKSEARHYQDYLK
                     MAQQYASEPIDERVAFFAEVEREAILTPDPEFRFHSGAAA"
     gene            complement(7680..9023)
                     /locus_tag="VIO83_05265"
     CDS             complement(7680..9023)
                     /locus_tag="VIO83_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY8330720.1"
                     /translation="MLARLALACLAGLASLPASALDLQEAWELLQAQGPTYRAAVHER
                     DAGVENRAIGRAGLLPQLSASASRSRVNGTREEKNFIGNTVESDLDYDAEAYIARLRQ
                     PLYNRQKFAEYRQGKQRADYSEAVFDAKTQQVAVSLAGRYFDVLLTRETIDLATAKLK
                     AFEQQVAAAQRRFDLGDGTITDVDQATARRDLAQAELIEAQDNQAVALRLLQEILGQV
                     PENLATLRQEFPTPALQPASLPEWLSQAKANNPSLRAQQFNHGVAEEEVNRAKSGHWP
                     TVDLVLGYTDSDSDSVSTIDQQNRYASAGVELNMPLYAGGGVSARVRQAVASREQAGE
                     ELNATREEVLSATTREFRGVQSGETRIRALERAVISSERAQDSARKSFLAGSSTNLDI
                     LNAEEQVYIARRDLLEAKLRYLQARLQLAAAVGLLGEDDISQANAYLGPALVLNN"
     gene            complement(9024..10370)
                     /locus_tag="VIO83_05270"
     CDS             complement(9024..10370)
                     /locus_tag="VIO83_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEY8330721.1"
                     /translation="MSNLGGEHMGLGLPGGERVIDVDDGRPRRWGLWLVAVGFGGFIL
                     WASLAPLDAGVVANATVNITDNRKTIQHLSGGSVNAILVREGDRVARDQPLIELDQTR
                     AIAEQGVVSSQYIVARTVENRLQAERDGLDTVTFDPELLARFAGDARLQEATTLQTRL
                     FATRRAALTGEAGILRENIRGAEEQLKGLRQVQAARSAQINFLAQELDGVRNLAAEGY
                     VARNRMLELERSAADLNAQRAQSVADIGRTHNQIAELKLRILQLEQDFQKEVQSQLTE
                     VQKEVSAQADRLRALDYEVAHTVIRSPIDGLVLGLNVSTIGGVIQPGAELMSVVPADE
                     PLQVDAMIPVQAIDKMAPGLPVDITFPAFNHAQTPNIPGRVLTIAADRLVDETSKEPY
                     YLAQIGVTEEGMGMLGSNQIRAGMPATVIIKTGERNLMSYLLKPVLTRVNSAFKEQ"
     gene            complement(10377..12074)
                     /locus_tag="VIO83_05275"
     CDS             complement(10377..12074)
                     /locus_tag="VIO83_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEY8330722.1"
                     /translation="MFRLRRTFYALAAFSGVINLLMLTPAVYMLQVYDRALVSSNVTT
                     LLMLTLLVVGLFVLIALLEVVRSSVLIRVGNRLDMMLNQRIFSAAFERNLRRAGGNPS
                     QALQDLSQVRQFLTGNGLFAFFDAPWTPIYLIVVSLVHPLLGVITLVGSLLLVSLAWL
                     TETSTRKPLAEANQAAMASATFANNNLRNAEVIEAMGMLPAIRQRWFGNHLRILELQS
                     LASDRAAWINGVTRFVRITLQSLLLGAGALLAIEGEITPGMMIACSILGGRALAPVEQ
                     VIATWKQLLSSRAAWGRLSTLLHDFPARKESMSLPKPTGMLSVESAFANAPGSNLPIL
                     RAISFNLSPGEALGVIGPSASGKSTLARLLVGIWPAQAGKVRLDGAEVFQWNKEELGP
                     WLGYLPQDVELFEGTIAENIARFGQVDSDAVIQAARQASVHEMILRLPQGYDTHLSTD
                     GGSLSGGQKQRIGLARALYGDPSLIVLDEPNSSLDDVGEAALIQALSDLKRRGKTLVL
                     ISHRPTVLNIVDKILVLREGMVHMFGSRDEVFAALRQASVAQAAAGATPLSSVKTKE"
BASE COUNT         1856 a         3828 c         4156 g         2237 t
ORIGIN      
        1 gagccgctgg ctcggccgcg ggtcgaggtt gcagtgtttg gcgccgggca tgtcggccgc
       61 gcgctggtgc cgctgctcgc cggcctgccg tgccgggtgc gctggatcga ttcgcgcgtg
      121 gccgagttcc cggcgcagct gccgccgggt gtcgagcggg tggtcagcga ggagccggtc
      181 gacgaggtgg acgagctgcc gcccggcagc tactgcatcg tcatgaccca cgaccatcag
      241 ctcgacctcg agctggccga gcgcatcctg cgccgcggcg acttcgtcta tttcggcctg
      301 atcggctcgc ggaccaagcg cgcgcgcttc gagcaccgcc tgcgcgagcg cggtctggcg
      361 gcggagtcga tcgcgcgcat gcgttgtccg ctggggttgc ccgaggtgcg cggcaagctg
      421 ccgctggaga ttgccgtgtc ggtggcgggg gaggtgatcg ccacctacag cgccgagcgg
      481 gacaggtcgg agccataggg cgccggctca ggtgtccggc ctttactcca gcccatccgc
      541 ttttccgcca ggctggcgcc ggcccggtat aatcggcgct tttgcgagta ttcccgtggc
      601 caacaagaga tacagctgta tcggtctgag caatcccaag tcgccggaaa atgtcggatc
      661 gatcatgcgc gcggcgggct gctatgaagt cagctcggtg ttctataccg gcacccgcta
      721 cgaccgggcg cgggacttcg tcaccgatac caagaaggtc catcaggata ttccgctgat
      781 caacatcgac gacctgcgca agattctccc cttcggctgc acgccggtgg cggtggaact
      841 ggtcgaaggt gcccgctccc tccccgagta cacccacccg gaccgggcgc tgtacatctt
      901 cgggccggaa gacggctcgc tggacaagca gatccgcgac tggtgcgagg acgtgatcta
      961 cattcccacc aacggctgca tgaacctcgc cgccacggtc aacgtggtgc tctacgatcg
     1021 cctggccaag ggcaacaata cccgctccgg gccgctgttc tagagcgctc gagccgtccg
     1081 ccagcgggag accgatagca ggccggttcc tgagccgttg ctcggccagt acccgcgcgg
     1141 ggcgcaatcc ccagggtttc gaaggctgcc cggcggggcg taatttgcta aggtgcgccc
     1201 gccgttccgt cgcccattcc cgagaaacct gcccctatgt ctgccgagca ctccagcact
     1261 gcgcccgtgg cgcgcccgtt gacccgcagc gacttcaaga ccctctcgct ctccgccctg
     1321 ggcggtgcgc tggagttcta cgacttcatc atcttcgtgt tcttcgcggc ggtggtcggc
     1381 aagctgttct tccccgccga gatgcccgag tggctgcgcc agctgcagac cttcggcatc
     1441 ttcgccgccg gctatctggc gcggccgttg ggcggcatcg tcatggccca cttcggcgac
     1501 ctgctcgggc gcaagcgcat gttcaccctg agcatcttca tgatggccgt gccgaccctg
     1561 tgcatgggcc tgctgccgac ctatgcgcag atcggcctgt gggcgccgct ggcgttgctg
     1621 gcgctgcggg tggtgcaggg cgcggcgatt ggtggcgaag tgccgggggc ctgggtgttc
     1681 gtcgccgagc atgtgccggc gcggcgcatc ggctttgcct gcagcaccct gactgccggt
     1741 ctgaccgccg gcattctgct cggctcgctg accgccagcg cgatcaaccg ggcgttcagc
     1801 gccgaggaac tggccgacta cgcctggcgc attcccttcc tgctgggtgg ggtgttcggg
     1861 ctggtctcgg tctacctgcg ccgctggctg cacgagaccc cggtgttcgc cgagctgcaa
     1921 ctgcgccagt cgttggccga ggaactgccg ctcaaggcgg tgatgcgcga gcatcgcccg
     1981 gcggtgctgt tgtcgatgct gctgacctgg gtgctgtcgg ccggcatcgt ggtggtcatc
     2041 ctgatgaccc cgacgatcct gcagaccctg cacggcttca ccccgggcga agcgctgcag
     2101 gctaacagcc tggccatcgt cgccctcagc ctcggctgcg tgctggccgg cctggcggcg
     2161 gaccgcttcg gcgcggggcc gacctttgtc ttcggcggcc tgctgctgtt gctgagttcc
     2221 gcgctgttct atgccggcat cgccagccat cgtgatctgc tgatcccgct gtacaccctg
     2281 gccggcctgg gcgtgggcac catcggcgcc atccccatgg tgatggtcaa ggccttcccg
     2341 gtggcggtac gcttctccgg ggtgtcgttc tcctacaacc tggcctacgc catctgcggc
     2401 ggtatgacgc cgatcctggt cagcctgctg ttgaagtgga gtccgctggg gcctggctac
     2461 tatgtcggcg cgctgagcct gctgttcatc ctcgccggcg tcggcctgtg gcgccgcgcc
     2521 gcgtcggcca tggcgcctgc cggctgcgct ccgtagcggg gcagcgggac tcttttggat
     2581 ggctgaaaaa aagaaacccc gccgaagcgg ggtttctttc agtgcctagg tactacagga
     2641 tcaagcctgg atgaccggga tgttggcctt ggcagccgcg tcacggtatt cagccatctg
     2701 atcgaaggac aggtagcggt aaacgtcggc agccatgctg tcgatgttct tcgcgtagcc
     2761 catgtactcc tcaacggtcg gcagcttgcc gatgatggag gcaacagccg ccagctcagc
     2821 agaagccagg aacacgttgg tagcgtcgcc cagacggttc gggaagttac gggtcgaggt
     2881 ggagaccacg gtcgaaccgg tttgtacgcg agcctggtta cccatgcaca gcgagcagcc
     2941 cggcatttcc atgcgcgcgc cagcacggcc gtagatgccg tagtagcctt cttcggtcag
     3001 ctggtgagcg tccatcttgg tcggcggagc cagccacaga cgggtcggga taccgccctt
     3061 gaccttgtcc agcagcttgc cggcagcgcg gaagtgaccg atgttggtca tgcacgaacc
     3121 gatgaacact tcgtcgatct tctgaccggc aacggtggac agcaggcggg catcgtccgg
     3181 gtcgttcgga gcgcagagaa ccggctcttt cacgtcggcc aggtcgatct cgatcacggc
     3241 cgcgtattcg gcgtccttgt cggcttccag cagcttcggg ttggccagcc aggcttccat
     3301 cgcctgggca cgacgctcca gggtacgggc atcgccgtag ccttcgccga tcatccagcg
     3361 cagcatggtg atgttggagc gcaggtattc ggcgatagcc tcttccggca gcttgatggc
     3421 gcaaccggca gcggaacgct cggcggaggc gtcggacagc tcgaaagctt gctcgacggt
     3481 cagaccgtcc agaccttcga tctcgaggat gcggccggag aagatgttct tcttgccctt
     3541 cttctcgacg gtcagcaggc cagcctggat cgcgtagtag gggatggcat ggaccaggtc
     3601 acgcagggtg atgcccggtt gcagcttgcc cttgaagcgc accagaacgg attccggcat
     3661 gtccagcggc atgacgccgg tggcggcggc gaaggcgacc agaccggaac cggccgggaa
     3721 ggagatgccg atcgggaagc gggtgtgcga gtcaccaccg gtaccgacgg tatccggcag
     3781 cagcatgcgg ttcagccagc tgtggatgat gccgtcgccc ggacgcaggg acacgccgct
     3841 gcggttcatg atgaagtccg gcagggtgtg gtgggtgacc acgtcgatcg gcttcggata
     3901 ggcggcggtg tggcagaagg actgcatcac caggtcggcg gagaagccca ggcaagccag
     3961 gtccttcagc tcgtcgcggg tcatcgggcc ggtggtgtcc tgggaaccga cggtggtcat
     4021 cttcggttcg cagtaggtgc ccggacgtac gccctggcct tccggcagac cgcaggcacg
     4081 gccaaccatc ttctgggcga gggagaagcc cttgccgctg tcagccggag caaccggctt
     4141 cttgaacagg gtggacggag ccaggcccag ttcggcacgc gccttctcgg tcagaccgcg
     4201 accgatgatc agcgggatac ggccgccggc gcggacttcg tccagcaaca cgtcggtctt
     4261 cagctggaag gtggtgacca gctcgtcgct atcgtgacgg cgcacttcgc ccttgtacgg
     4321 gtacacgtcg atgacgtcac ccatggccag gttggtgcag tcgaattcga tcggcagggc
     4381 gccggcgtct tccatggtgt tgtagaagat cggggcgatc ttggtgccga agcagaagcc
     4441 accggcgcgc ttgttcggca cgttcgggat gtcgtcaccg aagaaccaca gcacggagtt
     4501 ggtggcggac ttgcgggaag aaccggtgcc gaccacgtcg ccgacgtagg caaccgggaa
     4561 gcccttggcc ttcagctctt ccatctgctt cagcggaccg accgaacccg ggacgtccgg
     4621 gttgatgccg tcgcgggcca tcttcagcat ggccagggcg tgcagcggga tgtcagggcg
     4681 cgaccaggcg tccggagccg gcgacaggtc gtcggtgttg gtttcgcccg ggaccttgaa
     4741 cacggccagg gtgatcttct cggccagggc cggacggttg acgaaccact cggcgtcagc
     4801 ccaggactgc agcacggcct tggcggcggc gttgcccttc ttggcgcgct cggaaacgtc
     4861 gtggaaggcg tcgaacatca gcagggtgtg cttgagctga gcagcagcga cttcggccag
     4921 ttcggcgttg tccagcagct cgaccagggt ggcgatgttg tagccgccct gcatggtgcc
     4981 cagcagctcg acagcgcgct gcttgctgat cagcggagaa gcggtctcgc ccttggcgat
     5041 ggcggacagg aaaccggcct tgacgtaggc ggcttcgtcg acgcccgcgg gaacgcggtt
     5101 ggtgatcagg tcaaccagga attcttcttc gccggccggc gggttcttca gcagctcaac
     5161 caggcctgcg gtctgctcgg cgttcagcgg ctggggcacg acgccctggg cggcacgctc
     5221 ttctacgtgt ttgcggtagg cttcaagcac agtattaccc tcatcagtgg tcccatgcgg
     5281 gagtccggga ctttcgtcca gaagctgttt tcaaagtttt acgccggcga tcatgacgaa
     5341 ggtgtgttgg tgagggcgga gcgatgcggg tctcaccaac tgcttcctac cgggtttact
     5401 gtgctcgtga cgctttgaaa acagcttcct gcggacattg gcgcctgtaa caaggcgggc
     5461 agattctact ggaaagaccg gaataagtta agcaatccac ccagccggtg gcggatgacc
     5521 cagcatagac aaagggctaa catgccgcct catccccgtt tcgcacgcca tttgccatgc
     5581 cccatcagcg catcaagact ccctgtatcg gcctctgttc cacggtttat ggcgatctgg
     5641 tgtgccgcgg ctgcaagcgc ttccaccacg aggtggtgaa ctggaacctg tacggcgaag
     5701 acgagaagcg cgccgtctgg cagcgtctgg aaaccctgct ggtgcaggtg gtggccgcca
     5761 agctggaagt gttcgatccg cacttgctgc gccaccagct gcagcagcgg cagattcgct
     5821 tcgtcgagcg gcagtcgccg tactgctggg cctaccagct gatcgcccgc ggtgcgcgga
     5881 tgatccagca gctggacgcc tacggcatcg tgctgctgcc ggagttccgc gacagcagcc
     5941 tgccggagct gcgcgatgcg atcgatcgcg agtacttcct gctgtccgag gcccactacc
     6001 agcgctatat cgcgccgctt ttcttggtgg agggccggga ggggcgcggc tgatcgggct
     6061 tactccgtcg ccgtcagttt ctccagctgg tcgcagatgt cgtccggctt gagtaccagc
     6121 acgtcgcttt gcagtgtatc cagcaccact tcggcagtgt tgccgatcag cgcgccggac
     6181 aggccgctgc gtgccaccga gccgatcacc gtgactgccg catcgagctg caatgccatc
     6241 tgcggaatca gcaggtcggc ggggccctcg gcgatatgca gctggtggct gccgaggccg
     6301 tactcgtcct ggaagctctg gcaggcttcg cggtagcgcg cggcgatggt ttcgctcagc
     6361 tggaagctgg ggtcggcggc cgtgagcagc ggcgctggat gggcgctgag tacatgcagc
     6421 tcgcccttgg ccaggtgggc gatgtcgtgg gcgtggcaga cgatgtttgc atgcagggaa
     6481 cggtgctcgg gctcgtcatt gccgacatcg accgccgcca gcaccttgcc gccggtccag
     6541 ggccggtcgg tcttgaccag cagcaccggg cagggacagt agcgcagcaa cttccagtcg
     6601 tccggggtga gcaacgcctt cttcagcggg ttgtccgggt agtgctgctt gatcaccagg
     6661 ccgcagccct cggcctgctg agcggcgatg atggtctggt aggtcgtttc ctgccagtcc
     6721 tggcgggtgg agacggtgtg gccttcctcg cgcaggctca gcgccagatc gtcgagaaag
     6781 acgtcgtggt tgcgttgcgt gtcgcacacc agcaagtgca gctcggactg ggtggcgttg
     6841 gcgatcagcg ttgcccgttt gagcgccagg ccttccggct gattgggttc cagcactacc
     6901 agaatgctgc ggatggcttg catggtcttc ttcctcggtg gctttcgatc tcaactctag
     6961 acgcaacgcc gtcgtctgcc gcttgatgca cgtcaagcgg cagacatgcc cgtatcgagc
     7021 gagggccgta taatgcgcgg ctttccaaga ctagccggat tgccatgtcc ctgcttcccg
     7081 agatcgagag cttcctgctc tgcccgacgc cccaggcctg gatcgaccag gcgctgcagc
     7141 accccgagat cctgctgatc gatcacgcca actgcgagaa gaaggccgcc agtacggcgc
     7201 tgaccctgct gtaccgctat gtcgagaagg aagacctgca gtacaagttg tcgcgcctgg
     7261 cgcgcgagga gctgcgtcat ttcgagcagg tggcggcgct gatgaagaaa cgcggcgtgg
     7321 cctatcgccc ggtcagcgct tcgctgtacg cccagcgcct gcacgcgcac atccgcaaga
     7381 gcgagccggg caagctggtg gacaccctga tcgtcggcgc ctacatcgag gcgcgctcct
     7441 gcgagcgctt ctaccggctg gcgcctttcc tggacgagga gctgggcaag ttctacctct
     7501 cgctgctcaa gtccgaggcg cgccactatc aggattacct gaagatggcc cagcagtacg
     7561 ccagcgagcc gatcgacgag cgggtggcct tttttgccga ggtcgagcgc gaggcgattc
     7621 tgacgccgga cccggagttc cgcttccaca gcggcgcggc ggcctgatcc gcctgcgcgt
     7681 cagttgttga ggaccagcgc tgggcccagg taggcattgg cctggctgat gtcgtcctcg
     7741 ccgagcaggc cgacggcggc ggccagctgc agtcgcgcct gcaggtagcg cagcttggct
     7801 tcgagcaggt cgcggcgggc gatgtatacc tgttcctcgg cattgaggat gtcgaggttg
     7861 gtgctggagc cggcgagaaa gctcttgcgc gccgagtcct gggcccgttc gctggagatc
     7921 acggctcgct ccagcgcccg gatgcgcgtc tcgccgctct gcacgccgcg aaactcgcgg
     7981 gtggtggccg atagcacctc ctcgcgggtg gcgttcagct cctcgccggc ctgttcgcgg
     8041 ctggcgacgg cctggcgtac gcgggcgctg accccgccgc cagcgtacag cggcatgttc
     8101 agttccacgc cggccgaggc atagcggttc tgctggtcga tggtggagac cgagtcgctg
     8161 tcgctgtcgg tatagcccag caccagatcc acggtcggcc agtgtccgct cttggcccgg
     8221 ttgacttcct cttccgccac gccgtgattg aactgctggg cgcgcaggct cgggttgttg
     8281 gccttggcct ggctcagcca ctccggcagg ctggcaggct gcagggccgg cgtcgggaac
     8341 tcctggcgca gggtggcgag gttttccggc acctggccga ggatttcctg cagcaggcgc
     8401 agggcgactg cctggttgtc ctgcgcctcg atcagctcgg cctgggccag gtcgcggcgg
     8461 gcggtggcct ggtcgacgtc ggtgatggtg ccgtcgccca ggtcgaagcg ccgctgtgcc
     8521 gcggcgacct gttgctcgaa ggccttgagc ttggccgtgg ccaggtcgat ggtctcgcgg
     8581 gtcagcaaca catcgaaata gcggccggcc agactcacgg cgacctgctg ggtcttggcg
     8641 tcgaacaccg cctcgctgta gtcggcgcgc tgcttgccct ggcggtactc ggcgaatttc
     8701 tggcggttgt acaggggctg gcgcagacgt gcgatgtagg cttctgcgtc gtagtcgagg
     8761 tcgctctcga cagtgttgcc gatgaagttc ttctcctcac gggtgccgtt cacccggctg
     8821 cggctggcac tggcgctgag ctggggcagc aggccggccc ggccaatggc gcggttctcc
     8881 acgccggcgt cacgctcatg caccgcggca cggtaggtgg gtccctgggc ttgcaggagt
     8941 tcccaggcct cctgcaggtc cagagcgctg gcgggcaggc tggccaagcc ggccaggcag
     9001 gcgagggcga ggcgggcaag cattcattgc tccttgaagg cactgttgac gcgcgtcagc
     9061 accggcttga gcaggtagct catcaggttg cgctccccag tcttgatgat caccgtggct
     9121 ggcatcccgg cgcggatctg gttgctgccg agcatcccca tgccttcctc ggtgacgccg
     9181 atctgggcga ggtaataggg ctccttgctg gtttcatcga ccagcctgtc ggcggcgatg
     9241 gtcagcactc ggcccggaat gttgggggtc tgggcatggt tgaaggcggg gaaggtgatg
     9301 tccaccggca gtcctggggc catcttgtcg atggcctgca ccgggatcat ggcgtccacc
     9361 tgcagcggct cgtcggcggg caccacgctc atcagttccg cgcccggctg gatcacgccg
     9421 ccgatggtcg agacgttgag gccaagtacc aggccgtcga tgggtgagcg gatcacggtg
     9481 tgcgccacct cgtaatccag ggcccgcagg cggtcggcct gggcgctgac ctccttctgc
     9541 acttcggtca gctgcgactg cacttccttc tggaagtcct gctccagctg caggatacgc
     9601 agcttcagtt cggcaatctg gttgtgggtg cggccgatgt cggccacgct ctgcgcccgc
     9661 tgggcgttga ggtcggcggc gctgcgttcg agttcgagca tgcggttgcg cgccacataa
     9721 ccttcggcgg cgaggttgcg cacgccgtcc agctcctggg cgaggaagtt gatctgcgcg
     9781 ctgcgtgccg cctgcacctg gcgcaggccc ttgagctgtt cctcggcgcc gcggatgttt
     9841 tcccgcagga tgccggcctc gccggtcagc gccgcgcggc gggtggcgaa caggcgcgtc
     9901 tgcagggtgg tggcttcctg caggcgcgcg tcgccggcga agcgggcgag cagttcgggg
     9961 tcgaaggtca ccgtgtccag gccgtcccgc tcggcctgca ggcgattctc cacggtccgc
    10021 gcgacgatgt actgggaact gaccacgccc tgttcggcga tggcccgggt ctggtcgagc
    10081 tcgatcagcg gctggtcgcg cgccacccgg tcgccttcgc ggaccaggat ggcgttaacc
    10141 gaaccgccac tcaggtgctg gatggtcttg cggttgtcgg tgatgttgac cgtggcattg
    10201 gccaccaccc cggcatccag cggcgccagg ctggcccaga ggatgaagcc gccaaagccg
    10261 acggccacca gccacagtcc ccagcggcgc gggcggccat cgtcgacgtc gatcacccgt
    10321 tcgccacccg gaagccccag gcccatgtgt tctcccccca gattcgacat ggcgcatcac
    10381 tccttggtct tgaccgagga caggggcgtg gcgcccgcgg ccgcctgggc gacgctggcc
    10441 tggcgcaggg cggcaaacac ctcgtcgcgg ctgccgaaca tatgcaccat gccttcacgc
    10501 agcaccagga tcttgtcgac gatattcagt acggtgggcc ggtgcgagat cagtaccagg
    10561 gtcttgccgc gccgtttcag gtcgctcagg gcctggatca gcgcggcttc gccgacgtcg
    10621 tcgaggctcg agttcggctc gtcgagcacg atcagcgacg ggtcgccgta cagcgcacgg
    10681 gccagaccga tgcgctgctt ctggccgccg gacagcgagc cgccgtcggt gctgaggtgg
    10741 gtgtcgtagc cttggggcag gcgcaggatc atctcgtgca cgctggcctg gcgggccgcc
    10801 tggatcacgg cgtcgctgtc cacctggccg aagcgggcga tgttctccgc gatggtgcct
    10861 tcgaacagct cgacatcctg cggcaagtag cccagccagg ggccgagttc ttccttgttc
    10921 cactggaaca cctcggcgcc gtccaggcgc accttgccgg cctgggccgg ccagatgccg
    10981 accagcaggc gggcgagggt cgacttgccg gaggccgagg gaccgatcac gcccagggct
    11041 tcgccagggg acaggttgaa gctgatggcg cgcaggatcg gcaggttcga gcctggcgcg
    11101 ttggcgaagg cgctttccac gctgagcatg cccgtgggtt tgggcagcga catggactcc
    11161 ttgcgtgccg ggaagtcgtg cagcagggtg gacagtcgcc cccaagccgc gcggctggac
    11221 agcagctgct tccaggtcgc gatcacctgc tcgaccggcg ccagtgcccg gccgccaagg
    11281 atggagcagg caatcatcat ccccggggtg atctcgcctt cgatcgccag cagcgcgccg
    11341 gcaccaagga gcagcgactg cagggtgatg cgcacgaagc gcgtcacgcc gttgatccag
    11401 gcggcgcgat cagaggccaa actttgcagc tcgagaatcc gcagatgatt gccgaaccag
    11461 cgctggcgga tggccggcag catgcccatg gcctcgatga cttcggcgtt gcgcaggttg
    11521 ttgttggcga aggtggcgga ggccatggcc gcctggttgg cttcggccag aggcttgcgg
    11581 gtgctggtct cggtcagcca ggcgaggctg accagcaaca gcgagccgac cagggtgatg
    11641 accccgagca gcggatggac cagggagacg actatcaggt agatgggcgt ccagggcgcg
    11701 tcgaaaaagg cgaacaggcc gttgccggtg aggaactggc gcacctgcga cagatcctgc
    11761 agtgcctgcg atgggttgcc gccagcgcgg cgtagattgc gctcgaaggc ggcactgaag
    11821 attcgctggt tgagcatcat gtcgagccgg ttgccgacgc ggatcagcac gcttgagcgc
    11881 accacctcca gcagtgcgat cagcacgaac aggccgacta ccagcagggt gagcatcaat
    11941 agggtggtga cgttgctgct gaccagggcg cggtcgtaca cctgcagcat gtacaccgcc
    12001 ggagtcagca tcagcaggtt gatcaccccg ctaaaagcgg ccagggcata gaaggtgcga
    12061 cgcaagcgga acagtgc
//
LOCUS       DATCUN010000132         7412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_193455, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000132 DATCUN010000000
VERSION     DATCUN010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193455"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VIO83_05280"
     CDS             complement(<1..381)
                     /locus_tag="VIO83_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330723.1"
                     /translation="MPAISSDQPLYRIDECSDLMADACVCGDQGDLIFLSVWARDTAI
                     QQFIGRLTLGRSEDGLDQFHVITDQGGSLPVFVGSVERLEKRLTRSYRRTLFGSMVNL
                     WLFDRRCIRPDKSSASALALLPRSA"
     gene            complement(492..833)
                     /locus_tag="VIO83_05285"
     CDS             complement(492..833)
                     /locus_tag="VIO83_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330724.1"
                     /translation="MSQHHFETHHKGFPITVMLGWDRPLRYFFMTIRKPAELIDSTMK
                     VEDEDFLYSNLHEEDPFGHGLDYYRAVLGHFHIDVPSSMFTEVQKDYAGNVGNRVVKH
                     EADGSFAELRA"
     gene            complement(910..1317)
                     /locus_tag="VIO83_05290"
     CDS             complement(910..1317)
                     /locus_tag="VIO83_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330725.1"
                     /translation="MGWYFSSQSRSDLIAELVAPQETERVSAKVIAHTLRGNVLWSVV
                     EMTAKVEGVHRDLSPGQSLRYIRCDLLECSGGQWGYKPLDESMHPYYYSCPLSYLDLA
                     PEQSADWRAGVRAHHARRRTPTAAMASATALTV"
     gene            complement(1420..1761)
                     /locus_tag="VIO83_05295"
     CDS             complement(1420..1761)
                     /locus_tag="VIO83_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003116822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330726.1"
                     /translation="MSTIACDTPRSTLDEPAWQAVCKTAAEHAQRGCGLAWEHWVTLF
                     SSEIDAQVSRLPESQRVHALEIAAQEWDYATPAERQETQEWLAENGCCSHGIEFGYCP
                     AGCDRDDDDWD"
     gene            complement(1856..2545)
                     /locus_tag="VIO83_05300"
     CDS             complement(1856..2545)
                     /locus_tag="VIO83_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3275 family protein"
                     /protein_id="HEY8330727.1"
                     /translation="MAATSAPARSVLPIVVPGQLTLRTIRGKNGPFTVGRLTTPIGEF
                     AVKDAELEQYPEGKYDGEFVIRYIFAKSYPVAGGARFEVRANLDGMTLNGIEKLSRDE
                     ARSFATQEIDPLDEELGAQPAATPAKPARTSKPAKPAPVQASADPLVDTTPFGVDAPT
                     PAAAAATGSAEDGDAALFGILWPLGESVRLDSTIDRRTLRAQVARLGELGYRHQYQTQ
                     EWSRQAALEPA"
     gene            complement(2602..3519)
                     /locus_tag="VIO83_05305"
     CDS             complement(2602..3519)
                     /locus_tag="VIO83_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HEY8330728.1"
                     /translation="MNTTSNEKSYFDLHTSGIGYIQRVREVPVRGGRRAQPFLACTIA
                     ALVGSAKDPTYRYFDVKVSGADAKKLVERYIGVDDPKQRPLVRFRLGDLWGDAYIRDK
                     GEHAGKPGASLKARLLKAEPLDRAELASIRQHKLITRGIGYLSRPKDVTPKDGDPFLS
                     CCVAALAGPVGEPEYRYFDTIVATPEAEHLVRRCVQAIEGDRKVLIGFKLNDMKIDPY
                     IRTKGERAGEPGASLESTLVHIGLIKIDGKTVYSPSQAQAEAPQAEDAPAPEAEVAAD
                     TAADQPVEPAEREPVGDVEEQEPAMAESF"
     gene            complement(3783..4250)
                     /locus_tag="VIO83_05310"
     CDS             complement(3783..4250)
                     /locus_tag="VIO83_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HEY8330729.1"
                     /translation="MSLRRANAFVQEHHRHHRPVQGAKFALAVALSAIDGICGVAIVG
                     RPVARHLDDGWTLEVTRLCTDGAPNACSKLYGAAWKAAKALGYTRLITYTLPEEGGAS
                     LRAAGWRLLGTRGGGAWSRPSRPRADTPEHLRGPKCLWQAPGGADKGKHPDAD"
     gene            complement(4298..4894)
                     /locus_tag="VIO83_05315"
     CDS             complement(4298..4894)
                     /locus_tag="VIO83_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Nin-like protein"
                     /protein_id="HEY8330730.1"
                     /translation="MDFDRASRQGEPFEALIRKRQYLPNPVARGCTTSLKIRPMHRYL
                     RHLGWTDWDQMIGIRADEQRRVAKIRARGHSTESTHETMCMPLAEASVTVRDVSAFWQ
                     AQPFDLDLLTVNGRTLEGNCDLCFLKPRGQRLALIKARPEAAVWWIRMESLNLASKPS
                     GARFRADGPSYADLARFAADQGDLFDAAEEPIACFCGD"
     gene            complement(4919..5675)
                     /locus_tag="VIO83_05320"
                     /pseudo
     CDS             complement(4919..5675)
                     /locus_tag="VIO83_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611879.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(5721..5951)
                     /locus_tag="VIO83_05325"
                     /pseudo
     CDS             complement(5721..5951)
                     /locus_tag="VIO83_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Nin-like protein"
     gene            complement(6099..6452)
                     /locus_tag="VIO83_05330"
     CDS             complement(6099..6452)
                     /locus_tag="VIO83_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3085 domain-containing protein"
                     /protein_id="HEY8330731.1"
                     /translation="MSLRFKGCDLRPVLAEAVANQCRVVLAKDQGVYFLAEQGERRPD
                     GRVKLLAYAVGCNPDTDPFDDWWELARAELGGDDFGEFFDPKEGVFARILHGTNDLLL
                     SATTTHLSLEVVPSA"
     gene            complement(6534..6752)
                     /locus_tag="VIO83_05335"
     CDS             complement(6534..6752)
                     /locus_tag="VIO83_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330732.1"
                     /translation="MTSEANDCWVVYSPNESATSDSAGFWSNEFGWVQFDQATRFSLE
                     EALYAEIPVAVGRDARFVPWQEARQHYG"
     gene            complement(6749..>7412)
                     /locus_tag="VIO83_05340"
     CDS             complement(6749..>7412)
                     /locus_tag="VIO83_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY8330733.1"
                     /translation="KVVAANDDAIVVKLKPSEFAVLDPHLVTTVPSEGAKVHVQPYAR
                     RRFDGLRADIPEVITEKTADGVPYTITRHILGSAPAKLPIPEPQCMELGQLIQQLEEM
                     PAPDGFRRITHMLVDAGARDFTWVDPKPSKIIETPPAISFTVSTAKFEGRVTILYDRA
                     GDTYVVELHRDGELVDRHDEVYFDMLGAVLERLIDDGRWRLIDVSVIDAKATRRRQAV
                     AA"
BASE COUNT         1277 a         2287 c         2552 g         1296 t
ORIGIN      
        1 cgcggagcgc ggcagcagcg ccaaggcgct ggccgaactc ttgtcgggcc ggatgcagcg
       61 ccggtcgaac agccagagat tgaccatcga gccgaacagc gtgcggcgat agctgcgggt
      121 caggcgcttt tcaaggcgct ccaccgagcc gacgaacacg ggcagcgaac cgccctggtc
      181 ggtgatgacg tggaactggt ccaggccgtc ctcgctgcgg cccagggtga gccgcccgat
      241 gaactgctgg atggcggtgt cgcgcgccca gaccgagaga aagatcaagt cgccctggtc
      301 cccgcaaacg caggcatcgg ccatgaggtc gctgcattcg tcgatgcggt acaggggctg
      361 gtcggatgaa atggcaggca tggtggtttc ctcgatgtga ggggcgcacc gcccgcaggg
      421 gcagtgaatg ccccgttagg gatgaaacgc gcagcgccag ggcgaatgcc tcggctgcgt
      481 cgcaggtgac gtcaggctct cagttcagcg aacgaaccat cggcctcatg cttgacgacg
      541 cgattgccga cgttccccgc atagtccttc tggacttcgg tgaacatgct gctcggcacg
      601 tcgatgtgga aatggcccag gactgcacgg tagtagtcca gaccgtggcc gaacgggtct
      661 tcctcgtgca ggttgctgta caggaaatcc tcgtcctcga ccttcatggt gctgtcgatc
      721 agctcggcag gcttgcggat cgtcatgaag aaatagcgca gtggccgatc ccagccgagc
      781 atgacggtga tgggaaatcc cttgtgatgg gtctcgaagt ggtgctggct catggaggtg
      841 tccttggatg agctggaaca gcaccgcacg cgcgggcaag tgctgcccag gcgggtggaa
      901 ggaagagatt cagaccgtca gcgccgtagc ggatgccatg gcggcggtcg gggtacgccg
      961 tcgtgcgtgg tgggcgcgaa cgcctgcacg ccagtcggcg gattgctccg gtgcgagatc
     1021 cagataggac agcgggcacg agtagtagta cgggtgcatg gattcgtcca gcggcttgta
     1081 gccccactgg ccgccgctgc attcgagcag atcgcagcgg atgtagcgca gggactgacc
     1141 cggcgagaga tcacgatgca cgccttcgac cttggcggtc atttcgacca cggaccacag
     1201 cacgttgccg cgcagggtgt gggcgatgac cttcgcgctg acgcgctcgg tctcctgggg
     1261 tgcgaccagt tccgcgatca ggtcggatcg cgattgggaa gagaaatacc agcccatgag
     1321 aggcctcctg gaaagtagag ccggaggcct ccccgtgggg aagaccccca gcgggtgatg
     1381 gaatgccgca attgcggcga aggaacctat gctctgccct tagtcccagt cgtcatcgtc
     1441 acgatcacaa ccggccgggc agtagccgaa ctcgatcccg tgcgagcagc agccgttctc
     1501 ggcgagccac tcctgggttt cctgccgttc ggcaggtgtt gcgtagtccc attcctgggc
     1561 agcgatttcc agtgcatgaa cgcgctggct ctccggcaac cgactgactt gcgcgtcgat
     1621 ctcggaactg aaaagcgtga cccagtgttc ccaggccagg ccgcaaccac gctgggcgtg
     1681 ctcggcagcc gtcttgcaga cagcctgcca cgcgggttcg tccagcgtag aacggggggt
     1741 atcgcaagcg atggtggaca tgatggagac ctccagaaaa atgccggagc ctctcccgca
     1801 tggggaagga accccggcgg gtggatgaag aacaccgcgg atgcggcgtt tgcgatcacg
     1861 caggttccag tgcggcctga cggctccact cctgcgtctg gtactggtgc ctgtagccca
     1921 gttcgcccag gcgggcgacc tgcgcgcgca aggtgcgacg gtcaatggtc gaatccagtc
     1981 ggacggattc gcccagcggc cacaggatgc cgaacagtgc ggcgtcgcca tcttcggcgc
     2041 tgccggtggc agcagccgca gcgggcgtcg gcgcatccac accgaagggc gtggtatcga
     2101 ccagcgggtc cgcggatgcc tgcacgggtg cgggcttcgc aggcttggac gttctggcgg
     2161 gcttggccgg tgttgctgcc ggctgcgctc ccaactcttc atcgagcgga tcgatttcct
     2221 gggtggcgaa gctgcgggcc tcgtcacggg acagtttttc gatgccgttg agcgtcatgc
     2281 catcgaggtt ggcgcggact tcaaaccgcg cgccacccgc gaccggatag gacttcgcga
     2341 agatgtagcg gatgacgaac tccccgtcgt acttgccttc ggggtactgc tccagctccg
     2401 cgtccttgac ggcgaactcg ccgatggggg tggtcaggcg accgaccgtg aacgggccgt
     2461 tcttgccgcg gatggtccgc aatgtgagct gacccgggac gacgatgggc aagactgatc
     2521 tcgcaggtgc cgatgtggct gccatgatgg ttctccttgg gaaataggaa gaagcaaggc
     2581 cccgtgaggg gccatgccgg atcagaacga ctcggccatc gccggctcct gctcctcgac
     2641 gtcacctacg ggctcgcgct cggcgggctc gacaggctgg tcggcagcgg tgtcggcggc
     2701 aacctcggct tcgggcgcgg gtgcgtcctc ggcttgcggc gcttcggctt gcgcctggct
     2761 cggcgaatag accgtcttgc catcgatctt gatgagaccg atatggacca gcgtcgattc
     2821 caggctcgct cccggttccc cggcgcgctc gcccttggtg cggatgtacg gatcgatctt
     2881 catgtcgttc aacttgaagc cgatcagcac cttgcggtcc ccttctatgg cctgcacgca
     2941 ccggcgaacc aggtgctcgg cctcgggggt ggcgacgatg gtgtcgaagt accgatattc
     3001 cggttcaccg acaggcccgg ccagtgccgc gacgcagcac gacaggaacg gatcgccatc
     3061 cttgggggtg acgtccttcg gacggctgag gtagccgata ccgcgcgtga tcagcttgtg
     3121 ctgcctgatc gaagccagtt cggcccggtc aagcggctcg gccttgagca gacgcgcctt
     3181 gagactggcg cccggcttgc cggcgtgctc gcccttgtcg cggatgtacg catcgcccca
     3241 aaggtctccg aggcggaagc gcaccagcgg gcgctgcttg ggatcgtcaa cgccgatgta
     3301 gcgctcgacc agcttcttgg catccgcacc ggacaccttg acgtcgaagt agcggtaggt
     3361 cgggtccttg gcggaaccga ccagcgcggc gatggtgcat gctaggaagg gctgcgcgcg
     3421 gcggccgccc cggacgggaa cctcgcggac gcgctggatg tagccgatgc ccgaggtgtg
     3481 gaggtcgaaa tacgatttct cgttggacgt ggtgttcatg gtgaatctcc atagggatga
     3541 agcggagaca caccggccca cgcatgcggg gaaaggtgcg taaccccgcg gtgggttgat
     3601 aaggcgaaag catccgccac cagagactgg tggccgctcg cggacggatg cggtgcgagc
     3661 tgggtgatca acgcggtgga accgcgccgt agccttgaag atcgtggcta cctgggatgc
     3721 tgctgacggc tgtcagcgag gcatgggggc agcatgggtg gggttcgcgc gcgcggcagc
     3781 aatcaatcgg catccgggtg cttccctttg tccgcgccgc ctggcgcctg ccacaggcac
     3841 ttggggccgc gtagatgctc gggggtgtcg gcgcggggac ggctggggcg gctccaggcc
     3901 ccgccccctc gggtgccgag cagccgccaa ccggcggcgc gcaggctggc accaccttcc
     3961 tcgggcaagg tgtaggtgat cagccgcgtg tagcccagtg ccttggccgc cttccaggcg
     4021 gcgccgtaga gcttgctgca ggcgttcggt gcgccatcgg tgcagagccg ggtgacttcg
     4081 agcgtccagc cgtcatccag gtggcgggcg accgggcggc cgacgatggc cacgccgcag
     4141 atgccatcga tggcggacag cgcgacggcc aaggcgaact tcgcgccctg aactggccgg
     4201 tggtggcggt ggtgctcctg cacgaaggca ttggcacggc gcaaggacac cggaagtagt
     4261 cgcagcgatg gggaggccga ttgcccgtcc atggccgtca atcgccacag aagcaggcga
     4321 tgggttcctc ggcggcgtcg aacaggtcgc cctggtcagc agcgaaacgc gccaggtcgg
     4381 cgtagctggg gccatcggcg cggaaccggg cgccgctggg cttgctcgcc aggttcaggg
     4441 actccatgcg aatccaccag acggcggctt cgggccgggc cttgatgagg gccaggcgct
     4501 gcccgcgcgg cttgaggaaa cacaggtcgc agttgccttc gagcgtgcgc ccgttcaccg
     4561 tgaggaggtc gaggtcgaac ggctgcgcct gccagaaggc gctcacgtcg cgcacggtca
     4621 cactggcttc ggccaacggc atgcacatgg tctcgtgggt cgattctgtg gaatggccgc
     4681 gcgcgcgaat cttggcgacg cggcgctgct cgtccgcccg gatgccgatc atctggtccc
     4741 agtccgtcca gcccagatgc cgcaggtagc ggtgcatcgg gcggatcttc aggctggtcg
     4801 tgcagccgcg cgcaacggga ttgggcaggt actggcgctt gcggatcagc gcctcgaacg
     4861 gctcgccctg gcggctggcc cgatcaaagt ccaccagcgt gaagcctagg gcgtgtcatc
     4921 aaataagcca aacgacagag gcagccagat gaatggcgcc aaggaaggcc acggctgtct
     4981 tgtcgtagcg tgtggcgatg gctcggtact gcttcaggcg cgcaaagaag ttctcaatca
     5041 gatgacgcgc cttgtacaga tgccggtcat attcgcgttg cacggtgcgt gtgcgaatgc
     5101 tgggaatgac cacggccttg cctgcttgca atagaggctc caccactctt tgccgtgcgt
     5161 cataggcctt gtcggcaatg accatctgcg catcgatgtc cttgagcagg gcatcggcac
     5221 cctccaggtc tgaagcctgg cccgccgtaa gtacaaagcc cgtgggattg cccagcgcat
     5281 cgactgtggc gtggatcttc gtgctcaagc cccccggctg cgtccgatgg cttgctccga
     5341 agcccccctt ttgcaccagc gctgtgctgg tgagcccgca cgatggtggc atcgatcatg
     5401 gcgtattcgt tgtccgcgtt ctgggccaat gcttcaaaca ggcgttgcca cacaccgcgc
     5461 cggctccagc gcatatggcg cagatgcacc acgcggaaat ccccgaatcg ctcgggcaga
     5521 tcgcgccagg caatgcccgc acgatagcgg tagagcaccg catcgacgaa cagacggttg
     5581 tctttggctg gcgctccaac gtgcccggcc ctgccgggca acaggccttc gatctgagac
     5641 cactgtctat ctgtcagggc atatctgcgg ttcatgctcc tgataacgaa gcaccatcag
     5701 aatttgatga caggccctag ctccgtgggc aggtattcca gccagcgtat cggcacctgc
     5761 cagtgcgcgg cgcagtcgcg cacgaagcgc agggtcgctt ccacttcctt gccggtgttg
     5821 gcgaagcaga ccacggtgtc ggcaggcagg ccgccattgg cctgcaacac gcgccagagc
     5881 atgtaggcgc tggtacggcc gcccgagaag ctgatgcagg tcggttgggt gatcttgaac
     5941 gggtcgtgca tgcggtcgtc tccgtgggca tgtcggcggg catgccgacg aggcgggcca
     6001 aggcggccgg agacgaacgc gcacacgcag caacggccgc ggtgcggaga aaagaaagcc
     6061 cccatgaagg gggccgtgag gggacgggag tagccacgct acgccgatgg caccacctcc
     6121 agcgacaggt gcgtcgtggt ggcggacagc agcagatcgt tcgtgccgtg caggatgcgc
     6181 gcgaagacgc cttccttcgg gtcgaagaat tcgccgaagt cgtcgccgcc cagctcggcg
     6241 cgcgccagct cccaccagtc gtcaaacggg tcggtatccg ggttgcagcc gacggcatag
     6301 gccagcagct tcacgcggcc atcgggacgg cgctctccct gctcggcgag gaagtacacg
     6361 ccctggtcct tggcgaggac gacgcggcat tggttggcga cggcctcggc cagcacgggg
     6421 cgcaggtcgc agcctttgaa tcgcagtgac atagttgaaa tctcctgaag aaaaaggccc
     6481 tccacccgaa cggatggaag gcctgcgggg caagtcgccg gtaggctgcg gactcagccg
     6541 tagtgctgcc gggcttcctg ccacgggacg aagcgcgcat cccgaccgac tgcgaccgga
     6601 atctctgcgt acaaggcttc ttcgagcgag aagcgcgttg cctggtcgaa ctggacccag
     6661 ccgaactcgt tggaccagaa accggcgctg tcgctggtgg ccgactcgtt gggcgaatac
     6721 accacccagc agtcatttgc ctcggacgtc atgccgcaac cgcctggcgt cgccgggtgg
     6781 ccttggcgtc gatcacgctc acgtcgatca ggcgccagcg cccgtcgtcg ataagccgct
     6841 ccaacactgc accgagcatg tcgaaataca cctcgtcgtg ccgatcgacc agttcgccgt
     6901 cgcgatgcag ttccaccacg taggtgtcgc ccgcgcggtc gtacaggatc gtcacccggc
     6961 cctcgaactt cgcggtcgag accgtgaagc tgatcgccgg gggcgtttcg atgatcttgg
     7021 acggcttcgg atcgacccag gtgaagtcgc gggcgcccgc atcgaccagc atgtgggtga
     7081 tgcgccggaa cccgtcgggt gctggcatct cttccaactg ctggatgagc tgccccagtt
     7141 ccatgcactg cggctcggga atgggcagct tggcaggcgc ggagccgagg atgtgcctgg
     7201 tgatggtgta gggaacaccg tccgcggtct tctcggtgat tacttccggg atgtccgcgc
     7261 gcaggccgtc aaagcggcgg cgggcatacg gttggacatg caccttggcg ccttcgctgg
     7321 gaacggtagt caccaggtgg ggatcgagca ccgcgaactc gctgggcttg agcttgacga
     7381 cgatggcgtc gtcgttggcc gcgaccacct tg
//
LOCUS       DATCUN010000133        11097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_193480, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000133 DATCUN010000000
VERSION     DATCUN010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11097
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193480"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..518)
                     /locus_tag="VIO83_05345"
     CDS             complement(192..518)
                     /locus_tag="VIO83_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330734.1"
                     /translation="MNKPKHIRVESDQQLTSHREAGKLHLYSEGQINVRLSGIRSVDV
                     HAITDLETHQITRAFGSVSHFLRFANGGEVRFAYNAAGALLEFVANDVEVGVIGGDRI
                     VLRQPD"
     gene            complement(547..1662)
                     /locus_tag="VIO83_05350"
     CDS             complement(547..1662)
                     /locus_tag="VIO83_05350"
                     /inference="COORDINATES: protein motif:HMM:NF040400.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6396 domain-containing protein"
                     /protein_id="HEY8330735.1"
                     /translation="MPLLLAAVFVLLSLTACPMNHLPRNMKLKAFDPHRADFTCKYEA
                     DANPPFTAEADALFQQGMAVTSYDLVFEQRDYAKAAQLWKQAAGMGHWKAAMNLAGLH
                     ERGLGVPQDSEQAVLLVEGLMKQGVPAAFDKMGTYHQRGIGVKGDASRAYAFWQLAAD
                     MGSSAAQAYLGAKLKGTYDNPDQGFWGNREVALKMLECSFAQGNGDAAYTLALTITGS
                     DPSLGEDNARALRVYHEGVKLGCKDCARDLSVSFRAAEPLTGHTLDKPRADRYRLLRE
                     ALELNPDLRFPNLDKVLPLPPAALPMWDGKRETLLDAAKPLVAKPAEKPTPGAQRTGR
                     AHIPQGHVLRRQARPVVVEAGTPESDSHEAKGVVEHA"
     gene            complement(1685..3238)
                     /locus_tag="VIO83_05355"
     CDS             complement(1685..3238)
                     /locus_tag="VIO83_05355"
                     /inference="COORDINATES: protein motif:HMM:NF040400.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8330736.1"
                     /translation="MNHLPRNMKLKAFDPHRADFTCKYEADANPPFTPEANALFQQGM
                     TVTSYDLVFEQRDYAKAAQLWKQAAGMGHWKAAMNLAGLYESGLGVPQDSEQAVLLVE
                     GLMKQGVPAAFDKMGTYHQRGIGVRGDASRAYAFWQLAADMGSSAAQAHLGYKLQGTY
                     DNPDQGFWGNREVALKMLECSFAQGNGDAAHSLAQTITGSDSSLGEDNARALRIYHEG
                     VKLGCKDCARDLSSAFRRIEPLSGNTIDTVRADRYRVLREALELNPDLRFPNLDKVLP
                     LPPAALPMWDGKRETLVDAAKPLVAKPAEKPTPGAQRTGRAHIPQGHVLRRQARPVVV
                     EAATPVPHSGYWLARLAKVYRDHEQAWEDAQVPQRYAQGETFEAVDRSSMGKYAHYAP
                     RTVWHYLGEASPVPVVTHPRVAQGLARATRVPEPMLYCRGMLPCPRTGVWGGQVPRDH
                     PLARLYNRVDRYAYVEKGQPFPDPRHQHIPLAPHEVKWLWLDNANQPQRSGIVEVTLS
                     DLHDAQTAS"
     gene            complement(3334..5601)
                     /locus_tag="VIO83_05360"
     CDS             complement(3334..5601)
                     /locus_tag="VIO83_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY8330737.1"
                     /translation="MATPPKTQHKSVTPLSDASRSTTGSIQWKLESNEDARTPVYHYN
                     NLSVYICGEESFDKIAQDIRTAEKSVDIVCWGFDPAMELTRKKPGPWKRGDTYGDLLR
                     DAAEGKLNKSKSKVKVRLLTWYDPVALLLTDTNVPGYKKSASYELRAAPQQGFAAALA
                     PGGKPQALAEPTDVADQREVFNSRWWRDVVDGKIEGLSLRTRSGVHADVLAALQAEAR
                     QHGVAVGGIERFGLERLATHHQKTIVIDYEGTHPRAYVMGLNSVTDYWDTQEHLFNDP
                     KRGQLFEGGSADHSAGKGWDAPSADKTSLKPYQDYVCRIEGDALVAVYKNFVEAWNKA
                     KAEGRGGGTPVSADVDLKAPPRRLTQNLSAACNRAQIVRTLPDTEGGERSIERLYYQA
                     SSFARHYLYIENQYFQHTDWAHALKTQREYYVQGCRAARQPVAMSNVPVLHVMVVTPT
                     PERSQMVPRTHDIVTELGYGDSMPNQDKMIKDELDRHAQAERDLADFKRRRQAYDAGQ
                     SNEFQPPVFPGMAPPLSDLAQAHKAASEGKSGQDVRDMLSQALRMRTLVASLWTYDDE
                     WRVSKLPIAQRIDEEKQRYAQQKKEWDRYQAAQNKARADSINAGGWGTSTDTRVPPLP
                     PPDRSKELKNATAQRYREVYIHSKLMIIDDSMFTLGSANLNLRSFAVDSEINIASDDA
                     PTAKDLRQRVWQQHTKGQFDGGGDATDQKEISDTFDDWEREASDNLVKKTKGQSLSSF
                     LVKFLDERTSTIRFA"
     gene            complement(5619..8519)
                     /gene="tssI"
                     /locus_tag="VIO83_05365"
     CDS             complement(5619..8519)
                     /gene="tssI"
                     /locus_tag="VIO83_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEY8330738.1"
                     /translation="MSVHIAARTRTLEISSPAIPRFLGRPALEPVRLSGREGLNSLFE
                     YELLLKTPEDLNLGASGAADWDLDSFVGREISCTIQLDGMGEFLPGAVGASVDRVGAG
                     VRQINALITAACFWGEEGRHLQYKLTLRPWLHKATLRTNCRIFQNQSAVQILDELLAD
                     YAFPVDKRLIGLDGAEGGLYPTRDYQTQYNESDFDFFSRLCQEWGISYFFEHTAAEGG
                     SGTGQHRLVLVDNMGAYKPNDSAAYQEVAYHAPGWKVDAEYVHSLVPHSQLTSGQYTS
                     RDFDYTRPKADLTQSRKDPRPTGQADAQVYQWHASQGGSHYAQPRAGSAEANDPQAEG
                     RQLALMRMQALRTHGARAEASGNLRGMVPGCTFQLRKHPRQTANAEYLILDTRLLIED
                     VAQDSQIREAADGRKQHWKVEVDFTAHPMSEPLRPALTHFKPFTHGPQTALVVGPEGQ
                     NLWTDEFGRIKVQFPWDRLGQKNQHSTCWLRVSSPWAGNQLGGMQLPRIGQEVIVDYI
                     GGDPDLPVCTGRVHNQGNLPPWALPGQSALSGLRSRELTKEGGNSAAGRSNHLVLDDT
                     GGQIQAQLKSDHQHSQLSLGHITRIEDNAGRKDPRGQGFELRTDGHGVARAAAGLLLT
                     TEPRPRAQAHTTDMDETVARLTQGRDLQESLGAAAQEAKAHEPGDQDHVAEALKGQHD
                     AIRGMGQPGNTETGHFPELEEPHLVIASPAGIQSTSAQSTHVHAGEHVALTSGAHASV
                     SAARSFLVSARQAVRLFAYHAGMRLFAFGGDIDIKALKESISIIAKLNIVQVANRITI
                     TAKEELVLNGGGSTICLNAAGIEEATSGQHTAHAAGHSLVGPKNAPTVPVSGSASLKD
                     SLTARSFTLRSHPEDGRPVVTEPYTLYRDGAEVEKGVTDAQGRVFIADHKPGTGAYKI
                     KLGNGNEFELPVSARLEGADQQLGAKGYRAAQGDPKDRQHHHLHRQGGPDET"
     gene            complement(8762..9112)
                     /locus_tag="VIO83_05370"
                     /pseudo
     CDS             complement(8762..9112)
                     /locus_tag="VIO83_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611498.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9242..10018
                     /locus_tag="VIO83_05375"
     CDS             9242..10018
                     /locus_tag="VIO83_05375"
                     /inference="COORDINATES: protein motif:HMM:NF025027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEY8330739.1"
                     /translation="MILHQLRNRTCVNCNASVPFSILHASPAVSKKALGNGQIEWIEG
                     PLIRAMVVCGNCHQPSLVELKPRPNVHGHLMHSHPHIQASFLNRLKPLASIFQNAPEN
                     IKHHWAVLGNEPGTVIDDYFEVVQEFPSAAVQRPDSAALPENVNTQLNRLIKVLHEPV
                     AAMAHCRRTLEMACKEKLGEAAKGKRLYELIDATLQSVEATREIAEWAHTIRHLGNDA
                     AHANDSDPTEQEAHQAYEVVRLMLDLLFEYPARIRQLRRA"
     gene            10111..10428
                     /locus_tag="VIO83_05380"
     CDS             10111..10428
                     /locus_tag="VIO83_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330740.1"
                     /translation="MEPDTDHADLQATRQWQARFDLPGAPPLPPTPEFVPSPVAARSP
                     QERLMAYAGDPCPQTGRWQAPRLHNRIEAVERGQPMPGPANTETGAVIWYLVNEAGSA
                     PAP"
     gene            complement(10494..>11097)
                     /gene="fliA"
                     /locus_tag="VIO83_05385"
     CDS             complement(10494..>11097)
                     /gene="fliA"
                     /locus_tag="VIO83_05385"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HEY8330741.1"
                     /translation="GLLDASSRYEDGKGAQFETFVSQRIRGAMLDELRASDWGSRGLR
                     QSARAVEKAIHKLEHVLGRPPTEGEIAKEMKMELSDYQSLLQDVQGCQLLYVEDFAKG
                     EAENPFIDMHAQAASHDRGGSNDPLAQLLESGFRHQLIDAISTLPERDQLLLNLYYEE
                     ELNLREIGAILEVSQSRVCQLHSQAISRLRARLKDELKDE"
BASE COUNT         1672 a         3506 c         3753 g         2166 t
ORIGIN      
        1 gggctgcggc gacaggcgcg cgaggtgctg ccggctttgc agcaacggct cgggtggcgg
       61 tggcgggcgg tagtcatcgt gcgggctcga cgccggaagc ggcacctgcc gcggccgtgc
      121 ccggtcgacc tcgtccgaga ccacgtcgag aaagttgttg tccagtggcg cgctcatcgg
      181 gcaggtgccg gtcagtccgg ttgcctgagc acgatgcggt cgccgccgat cacgcccacc
      241 tcgacatcgt tcgccacgaa ctccagcagc gcacccgccg cgttgtaggc gaagcgcact
      301 tcgccgccgt tcgcgaaccg caggaagtgc gacaccgaac caaaggcgcg ggtgatctgg
      361 tgcgtctcca gatcggtgat ggcgtggaca tcgacgctgc ggatgccgct cagcctcaca
      421 ttgatctggc cctcggaata caggtgcaat ttgccggctt ccctgtgaga ggtcagttgc
      481 tggtcggatt cgacgcggat gtgcttgggc ttgttcatgg gttgaagtcc ttctcccgct
      541 tcatcttcat gcatgttcga caacgccctt ggcctcatgc gaatcgctct cgggggtgcc
      601 ggcttcgacc accacagggc gggcctgccg ccgcagcaca tgcccctgcg ggatgtgggc
      661 gcggccggtg cgctgggcgc cgggggtggg tttctcggcc ggtttggcga ccaggggctt
      721 ggcggcgtcc agcagggtct cgcgtttgcc gtcccacatg ggcaaggcgg cggggggcag
      781 cggcagcacc ttgtcgaggt tggggaagcg caggtcgggg ttgagttcga gggcttccct
      841 cagcaggcgg tagcgatccg cgcggggctt gtcgagcgtg tggcctgtca agggttcagc
      901 agcacggaaa ctaacggaca gatcgcgtgc gcaatccttg cagcccagtt tcacaccttc
      961 gtgataaaca cggagtgccc gggcgttgtc ttcgccaagt gaaggatcgc tcccagtgat
     1021 ggtcagggcc aacgtgtagg ctgcatcgcc attaccctgc gcaaaactgc actccagcat
     1081 cttcagcgcc acctcccggt tgccccagaa accctgatcg ggattgtcgt aagtgccttt
     1141 cagtttggcg cccaaatacg cctgcgccgc cgaactcccc atgtccgcgg ccagttgcca
     1201 gaacgcatag gcccggcttg catcgccctt cacgccgatg ccgcgctggt ggtaggtgcc
     1261 catcttgtcg aaggcggcgg gcacgccttg cttcatcagc ccttcgacca gcagcacggc
     1321 ctgttcgctg tcctgcggca cgcccaggcc gcgctcgtgc aggccggcca ggttcatcgc
     1381 ggccttccag tggcccatgc ccgcggcctg cttccacaac tgcgccgcct tggcgtagtc
     1441 gcgctgctcg aacaccaggt cgtagctggt caccgccatg ccctgctgga agagtgcgtc
     1501 ggcctcggcc gtgaagggtg ggttggcatc ggcctcgtac ttgcaggtga agtcggcccg
     1561 gtgtgggtcg aaggccttca gcttcatgtt gcggggcagg tggttcatgg ggcaggccgt
     1621 cagactcaac aggacgaaga cggccgccag cagcaacggc aacgcaggcc agcggcgatc
     1681 gtcatcagct tgccgtctgc gcatcatgca agtcgctcaa ggtcacctcg acgatgcccg
     1741 agcgctgggg ctggttggcg ttgtccagcc agagccactt cacctcgtgc ggcgcaaggg
     1801 ggatgtgctg atgccgggga tcggggaagg gctggccctt ctcgacatag gcgtagcggt
     1861 ccacacggtt gtagagccgg gccagcggat ggtccctggg cacctggcca ccccatacgc
     1921 cggtgcgcgg acagggcagc atgccccggc agtacagcat gggctcgggc acgcgggtgg
     1981 cgcgggcgag gccctgggcc acccggggat gcgtgaccac gggcacggga gaggcttcgc
     2041 ccaggtagtg ccagacggtg cggggcgcgt agtgggcgta cttgcccatg ctgctgcggt
     2101 ccacggcctc gaaggtttcg ccctgggcgt agcgctgcgg gacctgggca tcttcccagg
     2161 cctgctcgtg atcgcggtag accttggcca ggcgggcaag ccagtagccg ctgtggggca
     2221 cgggggtggc ggcttcgacg accacagggc gggcctgccg ccgcagcacg tggccttgcg
     2281 ggatgtgggc gcggccggtg cgctgcgcgc cgggagtggg tttctcggcc ggcttggcga
     2341 ccaggggctt ggcggcgtcc accagggtct cgcgcttgcc gtcccacatg ggcaaggcgg
     2401 cggggggcag cggcaatacc ttgtcgaggt tggggaagcg caggtcgggg ttgagttcga
     2461 gggcttccct caatactcga tagcgatcgg cacgcaccgt atcgatcgtg ttgccggaga
     2521 gcggttcaat tcggcggaaa gctgaagaga gatcgcgtgc gcaatcctta cagcccagtt
     2581 tcacgccctc gtgataaatg cggagtgcac gggcattgtc ttcgcctagc gaagaatcgc
     2641 tcccggtgat ggtctgtgcc aagctgtggg ccgcgtcacc attgccctgc gcaaagctgc
     2701 actccagcat cttcagcgcc acctcccgat tgccccagaa gccctgatcg ggattgtcgt
     2761 aagtgccctg tagcttgtag cctaggtgag cctgcgcggc cgaactcccc atgtccgctg
     2821 ccagctgcca gaacgcatag gcccggctcg catcgcccct cacgccgatg ccgcgctggt
     2881 ggtaggtgcc catcttgtcg aaggcggccg gcacgccttg cttcatcagc ccttcgacca
     2941 gcagcacggc ctgttcgctg tcctgcggca cgcccaggcc gctctcgtac agcccggcga
     3001 ggttcatcgc ggccttccag tggcccatgc ccgcggcctg cttccacaac tgcgccgcct
     3061 tggcgtagtc gcgctgctcg aacaccaggt cgtagctggt gaccgtcatg ccctgctgga
     3121 acagcgcgtt ggcttcaggc gtgaagggcg gattggcatc ggcctcgtac ttgcaggtga
     3181 agtcggcccg gtgcgggtcg aaggccttca gcttcatgtt gcggggcagg tggttcatgg
     3241 ggcaggccgt cagactgaat agaaggaaga caaggaccag cagcgaggcc ggccagcgaa
     3301 atggatggat gcgcggggca ggaagaggcg tgttcatgcg aatcggatgg tgctggtgcg
     3361 ctcgtcgagg aacttgacga ggaagctgga gagcgactgc cctttggttt ttttaactag
     3421 gttgtcgctg gcctcccgct cccagtcatc aaacgtatcc gaaatctcct tctgatccgt
     3481 cgcatcccca cccccatcga actgcccctt cgtatgctgc tgccacaccc gctggcgcag
     3541 gtccttcgcc gtgggcgcgt cgtcgctggc gatgttgatc tcgctgtcca ccgcgaagct
     3601 gcgcaggttc aggttggcgc tgcccagcgt gaacatgctg tcgtcgatga tcatcagctt
     3661 gctgtggatg tagacctcgc ggtagcgctg ggcggttgcg ttcttcagtt ccttgctgcg
     3721 gtcgggcggc ggcaagggcg gaacgcgcgt atcggtcgaa gtgccccagc cgccggcatt
     3781 gatgctgtcg gccctcgcct tattctgggc ggcctggtag cggtcccact ctttcttctg
     3841 ctgcgcatag cgctgctttt cttcgtcgat gcgctgcgcg atgggcagct tggagacgcg
     3901 ccattcgtcg tcataggtcc acaggctggc gaccagcgtg cgcatgcgca gcgcctggct
     3961 gagcatgtcg cgcacgtcct gtccgctttt gccctcgctg gccgccttgt gggcctgggc
     4021 caggtcggac aagggtggcg ccatcccggg aaacacgggc ggctggaact cgttgctttg
     4081 ccccgcgtcg taggcttggc gccgcctctt gaaatccgcc aggtcgcgct cggcctgggc
     4141 atgacgatcc agctcgtcct tgatcatctt gtcctggttc ggcatcgaat cgccatagcc
     4201 cagttcggtc acgatgtcgt gcgtgcgcgg caccatctgc gagcgctctg gcgtgggcgt
     4261 gaccaccatc acatgcagca cgggcacgtt ggacatggcc acgggctgcc tggcggccct
     4321 gcagccctgc acgtagtact cgcgctgggt tttcagggca tgggcccagt cggtgtgctg
     4381 gaagtactgg ttctcgatgt acaggtagtg gcgcgcgaag ctgctggcct ggtagtacag
     4441 gcgctcgatc gagcgctcgc cgccttcggt gtcgggcagc gtgcgcacga tctgcgcccg
     4501 gttgcaggcg gcgctgaggt tctgcgtgag ccgcctgggc ggcgccttca gatcgacatc
     4561 ggcactgacc ggcgtgccac cgcccctgcc ttcggccttg gccttgttcc aggcctcgac
     4621 aaagttcttg tacaccgcca ccagcgcatc gccttcgatg cggcagacat agtcctgata
     4681 cggcttgagc gaggtcttgt cggcgctggg ggcatcccac cccttgccgg ccgagtggtc
     4741 cgcgctgcca ccctcgaaga gctggccccg cttggggtcg ttgaagaggt gctcctgggt
     4801 gtcccaatag tcggtgacgg aattcaggcc catcacatag gcccgggggt gcgtgccttc
     4861 gtagtcgatc acgatggtct tttggtgatg ggtcgccagc cgctccagcc cgaatcgctc
     4921 gatgccgccc acggccacgc cgtgttggcg ggcctcggcc tggagggccg ccagcacgtc
     4981 ggcatgcacg ccgctgcgcg tgcgcagcga caggccctcg atttttccgt ccacgacatc
     5041 tcgccaccac cggctgttga acacttcccg ctggtcggcc acgtcggtgg gctctgcaag
     5101 tgcctggggt tttccgccag gcgccagcgc ggcggcaaag ccttgctgcg gcgccgccct
     5161 caactcgtag gatgcactct tcttgtagcc cggcacattg gtgtccgtga gcagaagagc
     5221 gaccgggtcg taccatgtga gcaggcgcac tttgactttg ctcttgctct tgttgagctt
     5281 gccctcggcg gcatcgcgca gcaaatctcc gtaggtgtcg ccgcgcttcc atggcccggg
     5341 ttttttgcgg gtcaattcca tcgcggggtc gaagccccag cacacgatgt cgacgctctt
     5401 ctccgcggtt cgaatgtctt gggcgatctt gtcgaagctc tcttcgccgc agatgtagac
     5461 cgagaggttg ttgtagtggt agaccggtgt cctggcgtcc tcgttgctct cgagtttcca
     5521 ttggatgctg ccggtggtgc tgcggctggc atcgctgagc ggggtgacgg acttgtgctg
     5581 cgtcttcggt ggggtggcca tggggatgtt gcctcgcttc aggtttcgtc ggggccgcct
     5641 tggcggtgca ggtggtggtg ctggcggtcc ttggggtcgc cctgtgcggc gcggtagccc
     5701 ttggcgccca gctgttgatc ggcgccttca aggcgcgcgc tgaccggcag ttcgaactcg
     5761 ttgccgttgc ccagcttgat cttgtaggcg cccgtgcccg gcttgtggtc ggcgatgaag
     5821 acgcggccct gcgcatcggt gacgcccttt tccacttcgg cgccgtccct gtagagggtg
     5881 tagggctccg tgacaacggg gcgtccgtcc tccgggtgcg agcgcagtgt gaagctgcgc
     5941 gccgtcaggc tgtccttgag gctggcgctt ccgctcaccg gcacggtggg cgcgttcttg
     6001 ggcccgacca ggctgtgccc ggcggcgtgc gccgtgtgct ggcccgaggt ggcctcttcg
     6061 atgccggcgg cattcaggca gatggtgctg ccgccgccgt tgagcacgag ttcttccttg
     6121 gcggtgatgg tgatcctgtt ggcgacctgg acgatgttga gcttggcgat gatgctgatg
     6181 ctttccttga gcgccttgat gtcgatgtcg ccgccgaacg cgaacaaccg catgccggcg
     6241 tggtaggcga acaggcgcac cgcctgcctg gcgctgacca gaaagctcct ggccgcagac
     6301 acgctggcat gtgcgccact ggtgagcgcc acgtgttcgc cggcatgcac atgggtgctt
     6361 tgcgcactgg tgctctggat gcctgcgggg gaggcgatca cgaggtgggg ctcctcgagt
     6421 tcaggaaagt ggcccgtctc ggtgttgcct ggctggccca tgccccggat cgcatcatgc
     6481 tggcctttga gggcctcggc gacgtggtcc tgatcgccgg gctcgtgcgc cttggcctcc
     6541 tgcgcggcgg cgcccagaga ctcctgcaga tcccggccct gcgtcaggcg tgccacggtc
     6601 tcgtccatgt cggtggtgtg ggcctgcgcg cgtggcctgg gctcggtggt gagcagcaag
     6661 ccggccgctg cgcgtgccac gccatgccca tcggtgcgca gctcgaagcc ttggccgcgc
     6721 gggtctttgc gcccggcgtt gtcttcgatg cgcgtgatgt gcccgaggct cagctggctg
     6781 tgctggtggt cgctcttgag ttgcgcctga atctggccgc cggtgtcgtc cagcaccagg
     6841 tggttgctgc ggcccgcggc ggagttgccg ccttccttgg tgagttccct ggaccgcaag
     6901 cccgacagcg cactctgccc cggcagcgcc cacggcggca ggttgccctg gttgtgcacc
     6961 cggcccgtgc acaccggcag gtcggggtcg ccgccgatgt aatcgacgat cacctcctgg
     7021 ccgatgcgcg gcaactgcat gccccccagc tggttgccgg cccatggact ggaaacccgc
     7081 agccagcagg tgctgtgctg gttcttctgg ccgaggcggt cccaggggaa ctgcaccttg
     7141 atgcggccga actcgtcggt ccacaggttc tggccctcgg ggccgactac cagggcggtc
     7201 tgggggccgt gggtgaaggg cttgaagtgc gtgagcgcgg ggcgcagcgg ctcggacatc
     7261 ggatgggcgg tgaagtcgac ctcgaccttc cagtgctgct tgcggccgtc tgccgcctcc
     7321 cggatctggc tgtcctgggc cacgtcctcg atcaggagcc gggtgtccag gatcaggtac
     7381 tcggcattgg ctgtctggcg cgggtgcttg cggagttgga aggtgcagcc cgggaccatg
     7441 cccctgaggt tgccgctggc ctctgcccgg gcgccatggg tgcgcaaggc ctgcatgcgc
     7501 atgagcgcca actggcggcc ttcggcctgc gggtcgttgg cttcggcgct gccggcgcgc
     7561 ggctgggcgt agtggctgcc gccctggctg gcatgccatt ggtagacctg cgcgtcggct
     7621 tggccggtgg ggcgcgggtc cttgcggctc tgggtcaggt cggctttggg gcgggtgtag
     7681 tcgaagtcgc gactggtgta ctggccgctg gtgagctggc tgtgcggaac gaggctatgg
     7741 acgtactctg catccacctt ccaccccggc gcgtggtacg ccacctcctg gtaggcggcg
     7801 ctgtcgttgg gcttgtacgc ccccatgttg tcgacaagca cgaggcggtg ctggccagtg
     7861 ccggagcccc cttcggcagc cgtgtgctcg aagaagtagc tgatgcccca ctcctggcac
     7921 aggcggctga agaagtcgaa gtcgctctcg ttgtactgcg tctggtagtc gcgggtgggg
     7981 tagaggccgc cttcggcacc gtccaggccg atgaggcgct tgtcgacggg aaaggcgtag
     8041 tcggccagca actcgtccag gatctgcacc gcgctctggt tctggaagat gcggcagttg
     8101 gtgcgcaggg tggccttgtg cagccagggg cgcaaggtga gcttgtactg gaggtggcgg
     8161 ccttcctcgc cccagaagca ggctgcggtg atgagggcgt tgatctggcg cacgccggcg
     8221 cccacgcggt cgaccgaggc gccgacggca ccggggagga actcgcccat gccatccagc
     8281 tggatcgtgc agctgatctc gcggccgacg aagctgtcga ggtcccagtc ggccgcgccg
     8341 ctggcgccga ggttcaggtc ctcgggggtc ttgagcagaa gttcgtattc gaacagcgaa
     8401 ttgaggcctt cgcggccgga caggcgaacc ggctcgaggg cggggcggcc caggaaccgg
     8461 gggatggcgg ggctgctgat ttcgagcgtg cgcgtgcgcg cagcaatgtg tacggacatg
     8521 acgattcctc ccaaggtgac tctgtggtct ggcgctgttc gatccgagcg ttctcaatct
     8581 gaatgccacg caagacagcg cctgcatggc cggattctgg gagcgaacga caaccaaatt
     8641 tgtcaacaaa tatcaactcg tgcgattcgc ctcaaaagtg ccacacatct cacacgggcc
     8701 accttgacga tgagtgctta ggttctcggc tggcgatcgg ctgcggggcg aggtgggccg
     8761 gtagcctgca ggcacgccgc tcgctcagat cgcattgctc gatcatggct gcgatggccg
     8821 cgcgcttggc ctgtgggacg agcgctttcc cctgaaggcc gtgttcagtg cgtggatgcc
     8881 caggtgggct tcgaccagca gcttcttgag cttgttgttc tcggcttcga gttcacgcaa
     8941 ccgccgggcg tcggacatgt tccatgcccc cgtacttggc gcgccattgc catttgtaga
     9001 aagtcacatc gctgaagccg cctttgcggc acagatcctt tacggccaag ccggcctcgg
     9061 actgcttgat gaaaccgatg atctgctcct gggtaaatct gctctttctc atgtccgtca
     9121 ttctccaagt tgacggactt cgctgccact acgctggtac agctggaagg gggctggtca
     9181 cgtgaacagg ccgccagcga aagtctcaaa tatcatcgcc ccagatacac cgagcaagct
     9241 catgatcctg catcaactca gaaatcgaac ctgcgtaaac tgcaacgcct ccgtcccctt
     9301 ttcgatcttg cacgcgtcgc cagccgtaag taagaaagct ctaggaaacg gacaaatcga
     9361 atggattgaa ggccctctca tccgcgccat ggtggtctgt ggaaactgcc accagccgag
     9421 tctggtggag ttgaagcctc gcccgaacgt gcacggacat ctgatgcaca gccacccgca
     9481 tattcaagcc agcttcttga acagactcaa acctctggcc tcgatctttc agaacgcccc
     9541 tgaaaacatc aaacatcact gggccgtcct cggcaacgag ccaggtactg tgatagatga
     9601 ctacttcgag gtggttcagg aattccccag tgcggccgtg caacgccctg atagcgccgc
     9661 gctcccagaa aacgtcaaca ctcaactcaa ccgactgatc aaggtactgc acgagccggt
     9721 cgccgccatg gcgcactgtc gtcgcacgct ggagatggcg tgcaaggaga aactcgggga
     9781 ggcggccaaa ggaaaacgcc tctacgaatt gatcgacgcc acgttgcaat cggtggaggc
     9841 gacccgagag atcgcagagt gggctcacac catccgtcat ttgggaaacg acgccgcgca
     9901 tgcgaacgac agtgacccca ccgagcagga ggcccaccag gcctatgaag tcgttcgact
     9961 gatgctcgat ctgttgttcg agtacccggc gcggatacgg caactgagac gggcgtgacc
    10021 gctggccgtg ataaagctca gacgcgttca gcgttacaaa agtccagcga tctccgggca
    10081 gcacccacgt tttccccaaa ggttcggaag gtggagcccg acactgacca cgccgacctc
    10141 caggccacgc ggcaatggca ggcgcgcttc gatctgcccg gcgcgccacc gctgccgccg
    10201 acgcccgaat tcgtgcccag cccggtcgct gcgcgcagcc cccaggaacg cctgatggcc
    10261 tacgcgggcg acccctgtcc gcagacgggc cgatggcaag caccgcgcct gcacaaccgc
    10321 atcgaagccg tcgagcgcgg ccagcccatg ccggggcctg ccaatacgga gacgggggcg
    10381 gtgatttggt atttggtgaa tgaggcgggt agcgctccgg ccccatgagc gggccggcgc
    10441 gggcaaggtc acccgacagg accggggagc gatagcttct cagcgccgag gcttcattcg
    10501 tccttgagct cgtccttcag gcgcgcgcgc agccggctga tcgcctggct gtgcagctgg
    10561 cacacgcgcg actggctcac ttcgagaatg gcgccgatct cgcgcaggtt cagctcttcc
    10621 tcgtaataca ggttcagcag cagctggtcg cgctcgggca gcgtggaaat cgcatcgatc
    10681 agctgatggc ggaagccgct ttccagcagt tgcgcgagcg ggtcgttgct gccgccacga
    10741 tcatggctcg ccgcctgcgc atgcatgtcg atgaacgggt tctccgcctc gcccttggcg
    10801 aagtcttcga cgtagagcag ctggcagccc tgcacgtcct gcagcagcga ctggtagtcg
    10861 ctcagctcca tcttcatttc cttggcgatc tcgccttcgg tcggcggacg cccgaggaca
    10921 tgctccagct tatggatcgc cttctcgacg gcgcgtgccg actggcgcag cccgcgcgag
    10981 ccccagtcgc tcgcgcgcag ctcgtcgagc atggcgccgc ggatgcgttg gctgacgaag
    11041 gtctcgaact gggcgccctt gccgtcttcg tagcggctcg acgcatcgag caggccg
//
LOCUS       DATCUN010000134         9294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_80544, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000134 DATCUN010000000
VERSION     DATCUN010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80544"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VIO83_05390"
     CDS             <1..1030
                     /locus_tag="VIO83_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8330742.1"
                     /translation="PAELEFHDLPQRVALAAGQGVELRYRIRPRVRGDSLFTRCEIGL
                     PSHLGLWQARRYLELPSRSRIYPDFARLHGARLMAVDSWLGRIGVRQQPRRGSGQEFH
                     QLREFRSGDSLRQIDWKATARKHQPIAREYQDERDQQIIFLLDSSRRMRSQDSDLSHF
                     DHALNACLLLAHVALRQGDAVGLLSFAGETPRYLPPAKGQQQLNALLNTVYDLQPGQL
                     PGDYQSAASELLARQPRRALVVLLSNLRDEDDQQLPAAAARIAQRHRLLIASLREEVL
                     DRQRQQPVQSYADALNYCGTVDYAGARVALHERLAAQGLPVLDVRPGELGPELVSRYL
                     QWKAAGTF"
     gene            1215..2135
                     /locus_tag="VIO83_05395"
     CDS             1215..2135
                     /locus_tag="VIO83_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8330743.1"
                     /translation="MQLKQLLVVIDPTQPSQPSLERAAWIARHSGAALELLLCEFNTA
                     LDAGVLFDSAKMDKARQSLLAHRREWLEQLAAPLRQEGLTVTCEARWGKPLHKMIITR
                     VQELKPDLVLKTAHSHGLLRRLLLSNSCWQLIRYCPAPLWLVKPEGKWLGQRLAVALD
                     PTHSADKPAALDHQLIETTQTLGQTLGLEDHYLHSYAPLPRTLVFDAELVADYEYYVE
                     HTGERHRLAFEQLLGQHAIAKPRTHLLQGFAEETLPRFVEDNAVDLLVMGAIARGHLD
                     TALIGHTAERVLEGVDCDLLVIKPQGVPAH"
     gene            2250..3701
                     /gene="pyk"
                     /locus_tag="VIO83_05400"
     CDS             2250..3701
                     /gene="pyk"
                     /locus_tag="VIO83_05400"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY8330744.1"
                     /translation="MPFRRTKIVATLGPSSSSPQVLEQLILAGMNVARLNFSHGTPDE
                     HKARAKLVRELAAKHDRHVALLGDLQGPKIRIAKFANKRIELQQGDAFRFSISHPRDA
                     GTQEVVGIDYPDLVKDCKVGDELLLDDGRVIMVVDQVTASELLCSVTVGGPLSDHKGI
                     NRRGGGLTAPALTEKDKADIKLAAEMELDYLAVSFPRDAADMEYARRLRDEAGSDAWL
                     IAKIERAEAVANDEALDGLIRASDGVMVARGDLGVEIGDAELVGIQKKIIAHARTLNK
                     VVITATQMMESMIHSPMPTRAEVSDVANAALDYTDAVMLSAESAAGDYPVEAVKAMAR
                     VCVGAEKHPTSKKSGHRMGETFTRCDESIALAAMYTANHFPGVKAVIALTESGYTPLI
                     MSRIRSAVPIFAYSPHRATQARASLFRGVQTIPFDAAALAPEQVSQAAVDELVKRGVV
                     QPGDWVIMTKGDSFDTIGGTNTLKILHVGEPLA"
     gene            3900..5423
                     /locus_tag="VIO83_05405"
     CDS             3900..5423
                     /locus_tag="VIO83_05405"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEY8330745.1"
                     /translation="MAVIKQDDLIQSVADALQFISYYHPVDFIQAMHEAYLREESPAA
                     RDSIAQILINSRMCATGHRPICQDTGIVTVFVRVGMDVQWTGATMSLDDMINEGVRRA
                     YNNPDNVLRASILADPAGARKNTKDNTPAVIHYSIVPGDKVEVDVAAKGGGSENKSKM
                     AMLNPSDSIVDWVLKTVPEMGAGWCPPGMLGIGIGGTAEKAALMAKEVLMESIDIHEL
                     KARGPQNKIEEMRLELFDKVNQLGIGAQGLGGLTTVLDVKIMDYPTHAASLPVCMIPN
                     CAATRHAHFVLDGSGPASLEAPSLDAYPDIVWEAGPSARRVNLDTITPEEVQSWKPGE
                     TILLNGKMLTGRDAAHKRMVDMLNAGEELPVDLKGRFIYYVGPVDPVRDEVVGPAGPT
                     TATRMDKFTRQILESTGLLGMIGKSERGPIAIDAIRDNKAVYLMAVGGAAYLVAQAIK
                     KAKVLAFAELGMEAIYEFEVKDMPVTVAVDTKGESVHITGPAIWKQKIADSLAVEVK"
     gene            complement(5637..6791)
                     /locus_tag="VIO83_05410"
     CDS             complement(5637..6791)
                     /locus_tag="VIO83_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEY8330746.1"
                     /translation="MTQAVFPWRGGNRFQLLIDGEQFFPRMIAAIDAACGQVELELYL
                     AEAGSCASRLVDCLCRAAQRGVQVRCLFDAFGSQRFGARLSGQLRRAGVQLRLYNPIA
                     WRHGVRNLYRDHRKLLLVDQQLAWVGGAGATDDFWLPEPAASRWHELMVEMAGPVVAD
                     WQILFEREWEACVEEHDWHPYIRPVAHLPARPLPGEGLGRVAYADSRQHRDILRSLLR
                     TLGHARGRIWLATPYFLPSWRVRKALRKAAQRGVDVRLLLSGRLTDHGPVRFAGHRYY
                     PRLLKSGVRIFEYQPRFLHLKAVLVDDWVSIGSCNFDHWTLRFNLEANLEALDPGLTA
                     ALAASLESGYALSREITLDDWFARPLAMRISQRLWGWLDRLVVNLMDRWR"
     gene            complement(6969..7754)
                     /locus_tag="VIO83_05415"
     CDS             complement(6969..7754)
                     /locus_tag="VIO83_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8330747.1"
                     /translation="MRREPIVLFDNGTHQCLCFDDLVTGEGVQTNQFLITDNEQYMLL
                     DPGGDLTYTPLSLELSKHIPVQDLTYIFASHQDPDIIAALDKWLLHTKAKVICSRLWA
                     RFLPHLTANYLAVSHGISTYDRIIALPDRGQSLPLGNCQLKAVPAHFLHSVGNFQLYD
                     PVSKILFSGDMGASLVEDAQPVSDFAAHLQSMEGFHRRYMASNKVCRLWADMVRKMDV
                     SMIVPQHGRPFVGPAMITAFLDWISQLECGMDLMGPADYTLPR"
     gene            8004..8759
                     /gene="olsB"
                     /locus_tag="VIO83_05420"
     CDS             8004..8759
                     /gene="olsB"
                     /locus_tag="VIO83_05420"
                     /EC_number="2.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ornithine N(alpha)-acyltransferase"
                     /protein_id="HEY8330748.1"
                     /translation="MSAIALSCESSPSRRLQAERLQGTESLIEAQALRYRVFGEEFGA
                     RLQGAEQGLDCDGFDAHCSHLGVRDLASGELVATTRLLDHATARQLGRFYSEDEFRLH
                     GLSKLQGPILEIGRTCVAAEYRNGATIAVLWGALAETLNEGNYRYLMGCASIPMRDGG
                     IQARAVMQRLRERHLCHDHLRAEPRHPLPALDVPDNLTAELPPLLKAYMRLGAKICGE
                     PCWDPDFQVADVFILLKRDELCPRYARHFKAVG"
     gene            8759..>9294
                     /locus_tag="VIO83_05425"
     CDS             8759..>9294
                     /locus_tag="VIO83_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8330749.1"
                     /translation="MRRLRLYARLLRLFLVLGEGALFAAWVELRERLGGAPMALRQRL
                     TWRFMRHLRAALPFTVQIHGRLPDTPVLWLANHVSWVDIAVLGALRPLSFLAKSEVGD
                     WPLAGWLARQAGTLFIRRGAGDSLQLGRQLAEQLAQQRHLAIFPEGTSSDGSAVLRFH
                     PRLLAAAVDSGAALQPVA"
BASE COUNT         1616 a         3348 c         2984 g         1346 t
ORIGIN      
        1 gccggccgag ctggagttcc acgacctgcc gcagcgcgtc gcactggccg ccgggcaggg
       61 cgtcgagctg cgctaccgca tccgcccgcg ggtgcgcggc gacagcctgt tcacgcgctg
      121 cgagatcggc ctgcccagcc acctcggcct gtggcaggcg cgccgctatc tggagctgcc
      181 cagccgctcg cgcatctatc ccgacttcgc ccgcctgcac ggtgcgcggc tgatggcggt
      241 ggacagctgg ctcggccgca tcggcgtacg ccagcagccg cgccgcggca gcggccagga
      301 attccaccag ctgcgcgagt ttcgcagcgg cgacagcctg cgccagatcg actggaaggc
      361 caccgcgcgc aagcaccagc cgatcgcccg cgaataccag gacgagcgcg accagcagat
      421 catcttcctg ctcgacagca gccggcgcat gcgcagccag gacagcgacc tcagccactt
      481 cgaccatgcc ctcaacgcct gcctgctgct cgcccatgtc gccctgcgcc agggcgatgc
      541 ggtcggcctg ttgagctttg ccggcgagac gccgcgctac ctgccgccgg ccaagggcca
      601 gcagcagctc aacgccctgc tcaacaccgt ctacgacctg cagccgggcc agttgccggg
      661 cgactaccag agcgccgcca gcgagctgct cgcgcgccag ccgcgccggg cgctggtggt
      721 gctgctgagc aacctgcgcg acgaggacga ccagcagctg cccgccgctg ccgcgcgcat
      781 cgcccagcgc caccgcctgc tgatcgccag cctgcgcgag gaagtcctcg accgccagcg
      841 ccagcagccg gtgcagagct acgccgacgc cctgaactac tgcggcaccg tcgactacgc
      901 cggcgcccgc gtcgccctgc acgaacgcct ggccgcccag ggcctgccgg tgctcgacgt
      961 gcgccccggc gaactcggcc cggagctggt cagccgctat ctgcagtgga aggccgccgg
     1021 caccttctga gcgcggccgc cagccccctg ctgccgttgc gcgcccggct gctgtcagcc
     1081 gctctacagc gcctgtcacg ccactgtcac cggtttgcat gtgtagtggc acaggcgcta
     1141 ggctgaacga attcaagccg ctccccgcgc ctgcgcgcca gcgggaacac cactgaccgg
     1201 ccaaggaatc cgacatgcaa ctcaagcagc tgctggtggt catcgacccc acccaacctt
     1261 cgcaaccatc gctcgagcgc gcagcctgga tcgcccgcca cagcggtgcg gccctggagt
     1321 tgctgctgtg cgaattcaac actgccctgg atgccggcgt gctgttcgac agcgccaaga
     1381 tggacaaggc gcgccagtcc ctgctcgccc accgccgcga gtggctggaa cagctggccg
     1441 caccgctgcg ccaagagggc ctgacggtca cctgcgaggc ccgctggggc aagccgctgc
     1501 acaagatgat catcacccgc gtccaggagc tcaagcccga cctggtgctg aagaccgccc
     1561 acagccacgg cctgctgcgt cgcctgctgc tgagcaacag ctgctggcag ctgatccgct
     1621 actgcccggc accgctgtgg ctggtcaagc ccgagggcaa gtggctgggc cagcgcctgg
     1681 ccgtggcgct cgacccgacc cacagcgccg acaagccggc cgccctcgac caccagctga
     1741 tcgagaccac gcagacgctg ggccagaccc tcggtctgga agaccattac ctgcacagct
     1801 atgcgccgct gccgcgtacc ctggtgttcg atgccgagct ggtagccgat tacgagtact
     1861 atgtggagca cactggcgaa cgccatcgtc tggccttcga gcagctgctc ggccagcacg
     1921 ccatcgccaa gccacgcacc cacctgctgc agggctttgc cgaagagacc ctgccgcgct
     1981 tcgtcgagga caatgccgtc gacctcttgg tgatgggtgc catcgcccgc ggccacctcg
     2041 ataccgcgct gatcggccat accgccgagc gggtgctcga aggcgtcgac tgcgacctgc
     2101 tggtgatcaa gccgcagggc gtacctgccc actgagccgg attccggcca ccaacgatcc
     2161 cggcctgccc aggcaggccg accaccactg acgtgcgccg ccacaagcgg gagcgccagc
     2221 gctttaccac cttacccagg agtaccctga tgccgtttcg ccgtaccaag atcgtcgcca
     2281 ccctcggccc gtccagcagc tcgccgcagg tgctcgagca gctgatcctc gccgggatga
     2341 acgttgcccg cctgaacttc tcccacggca ctccggacga gcacaaggcg cgcgccaaac
     2401 tggtccgcga gctggcggcc aagcacgatc gccacgtcgc cctgctcggc gacctgcaag
     2461 gcccgaagat ccgcatcgcc aagttcgcca acaagcgcat tgagctgcag caaggcgacg
     2521 ccttccgctt ctcgatcagc cacccgcgcg acgccggcac ccaggaagtg gtcggcatcg
     2581 actacccgga cctggtcaag gactgcaagg tcggcgacga gctgctgctc gacgacggcc
     2641 gggtgatcat ggtcgtcgac caggtcaccg ccagcgagct gctctgctcg gtgaccgtcg
     2701 gcggcccgct gtccgaccac aagggcatca accgtcgcgg cggcggcctc accgcgccgg
     2761 cgctgaccga gaaggacaag gccgacatca agctggccgc cgagatggag ctggactacc
     2821 tggccgtgtc cttcccgcgc gacgccgccg acatggaata cgcccgccgc ctgcgcgacg
     2881 aggccggcag cgatgcctgg ctgatcgcca agatcgagcg cgccgaggcg gtggccaacg
     2941 acgaggcgct ggacggcctg atccgtgcca gcgacggcgt gatggtggcg cgcggcgacc
     3001 tcggcgtgga gatcggcgat gccgagctgg tcggcatcca gaagaagatc attgcccacg
     3061 cccgcaccct caacaaggtg gtgatcaccg ccacccagat gatggagtcg atgatccaca
     3121 gcccgatgcc gacccgcgcg gaagtctccg acgtggccaa cgccgcgctg gactacaccg
     3181 acgcggtgat gctgtcggcc gagagcgccg ccggcgacta cccggtggaa gcggtcaagg
     3241 ccatggcgcg cgtctgcgtc ggtgccgaga agcacccgac cagcaagaag tccggccacc
     3301 gcatgggcga gaccttcacc cgctgcgacg aaagcatcgc cctcgccgcc atgtacaccg
     3361 ccaaccactt ccccggcgtc aaggcggtca tcgccctcac cgaaagcggc tacaccccgc
     3421 tgatcatgtc gcgcatccgt tcggcggtgc cgatcttcgc ctactcgccg caccgcgcca
     3481 cccaggcccg cgcctcgctg ttccgcggcg tgcagaccat ccccttcgat gccgcggcac
     3541 tggccccgga gcaggtcagc caggcggcgg tcgacgaact ggtcaagcgt ggcgtggtcc
     3601 agccgggcga ctgggtgatc atgaccaagg gtgacagctt cgacaccatc ggcggcacca
     3661 acaccctgaa gatcctccac gtcggcgaac cgctggcctg atcggcagcc tgtgacacag
     3721 aaggccgctc attcgagcgg ccttctgctt ttgcggcaga ccggcccgcg cccatcgacc
     3781 aaggtcgacg gcaggcaggt gtccgcaccg ccgcctgcgc gctatactgc ggcgccgttt
     3841 ttttgcgccg cctgcggtgg cgcgccgtga agttccgcct gtttagagga ccgcgttcca
     3901 tggccgtgat caagcaagac gacctgatcc agagcgtcgc cgacgccctg cagttcatct
     3961 cctactacca ccccgtcgac ttcatccagg cgatgcacga ggcctacctg cgcgaggaat
     4021 cgccggccgc gcgcgactcc atcgcccaga tcctgatcaa ctcgcgcatg tgcgccaccg
     4081 gccaccgccc gatctgccag gacaccggca tcgtcaccgt gttcgtgcgc gtcggcatgg
     4141 acgtgcagtg gaccggcgcg accatgagcc tggacgacat gatcaacgag ggcgtgcgcc
     4201 gcgcctacaa caacccggac aacgtcctgc gcgcctcgat cctggccgac ccggccggtg
     4261 cccgcaagaa caccaaggac aacaccccgg cggtcatcca ctactccatc gtccccggcg
     4321 acaaggtgga agtggacgtc gccgccaagg gcggcggctc cgagaacaag tcgaagatgg
     4381 ccatgctcaa cccgtccgac tcgatcgtcg actgggtgct gaagaccgta ccggaaatgg
     4441 gcgccggctg gtgcccgccg ggcatgctcg gcatcggcat cggcggtacc gccgagaagg
     4501 ccgcgctgat ggccaaggaa gtgctgatgg agtccatcga catccatgag ctgaaggccc
     4561 gcggcccgca gaacaagatc gaggaaatgc gcctcgagct gttcgacaag gtcaaccagc
     4621 tgggcatcgg cgcccagggc ctcggcggcc tgaccaccgt gctcgacgtc aagatcatgg
     4681 actacccgac ccacgccgcc tcgctgccgg tgtgcatgat ccccaactgc gccgccaccc
     4741 gccacgccca cttcgtgctc gacggctccg gcccggccag cctggaagcg ccgtcgctgg
     4801 acgcctaccc ggacatcgtc tgggaagccg gcccgagcgc gcgccgcgtg aatctggaca
     4861 ccatcactcc ggaagaagtg cagagctgga agccgggcga gaccatcctg ctcaacggca
     4921 agatgctcac cggccgcgac gccgcgcaca agcgcatggt cgacatgctc aacgccggcg
     4981 aagagctgcc ggtcgacctc aagggccgct tcatctacta cgtcggcccg gtcgatccgg
     5041 tgcgtgacga agtggttggc ccggccggcc cgaccaccgc gacccgcatg gacaagttca
     5101 cccgccagat cctggaaagc accggtctgc tgggcatgat cggcaagtcc gagcgcggcc
     5161 cgatcgccat cgacgcgatc cgcgacaaca aggccgtgta cctgatggcc gtcggcggcg
     5221 ccgcctacct ggtcgcccag gcgatcaaga aagccaaggt gctggccttc gccgaactcg
     5281 gcatggaagc gatctacgag ttcgaggtca aggacatgcc ggtcaccgtg gccgtcgaca
     5341 ccaagggcga gtcggtgcac atcaccggcc cggcaatctg gaagcagaag atcgccgaca
     5401 gcctggcggt ggaagtgaag taagcttccc cagccacagg aaaaccccgg accggcctcg
     5461 ccgctccggg gttttctttt tacaggtcgt tgaaaaacgt cgcgagcgaa ggtcaggcaa
     5521 ggcctcggcg gccccgcgaa aagtggcgag gaagcggagt ttacgaagtg taaatgagca
     5581 ctccgagcca ctttttaacg cagcctcacc gagcgcagca gtttttcagc ggcctgtcag
     5641 cgccagcgat ccatcagatt gaccaccagc cgatccagcc agccccacag ccgctgcgag
     5701 atgcgcatcg ccagcggtcg ggcgaaccag tcgtcgaggg tgatctcgcg gctgagcgcg
     5761 tagccacttt ccaggctcgc cgccagcgcc gccgtcaggc cgggatcgag cgcctcgagg
     5821 ttggcctcca ggttgaagcg cagggtccag tggtcgaagt tgcacgagcc gatactgacc
     5881 cagtcgtcca ccagcaccgc cttgagatgc aggaagcgcg gctgatactc gaagatgcgc
     5941 acgccgcttt tgagcagccg cgggtagtaa cggtggccgg caaagcgcac ggggccatgg
     6001 tcggtcaacc ggccactgag cagcaggcgc acgtccacgc cgcgctgcgc cgccttgcgc
     6061 agcgccttgc gcacccgcca gctgggcagg aaatacggcg tggccagcca gatacgaccg
     6121 cgggcatgcc ccagggtacg cagcagcgag cgcaggatgt cgcggtgctg acgcgaatcg
     6181 gcgtaggcca cccggcccag cccctccccg ggcagtggtc tggccggcag atgggcgacc
     6241 ggtcggatgt acgggtgcca gtcatgctcc tcgacacagg cctcccactc gcgctcgaac
     6301 aggatctgcc agtcggccac caccgggccg gccatctcca ccatcagctc gtgccagcga
     6361 ctggccgccg gttccggcaa ccagaagtcg tcggtggccc cggcaccgcc gacccaggcc
     6421 aactgctgat cgaccagcag cagcttgcga tggtcgcggt acaggttgcg caccccgtga
     6481 cgccaggcaa tcgggttgta caggcgcaac tgcacgccag cgcggcgcag ttgcccgctc
     6541 agccgcgcgc cgaagcgctg gctgccgaag gcatcgaaca ggcagcgcac ctgcaccccg
     6601 cgctgcgcgg cacgacacag gcagtcgacc agacggctgg cgcagctgcc cgcctcggcc
     6661 agatacagct ccagctcgac ctggccgcag gccgcgtcga tggcggcgat catccgggga
     6721 aagaactgtt cgccatcgat cagcaactgg aaacggttgc ccccgcgcca gggaaagacg
     6781 gcctgggtca tggcgccgaa gccatacagg ggcgggaagg ggccgggctg ggcatcgagt
     6841 cctcgtgcgg caatgaactc cctgcaccat agccggccgt tcgtcggggc tcaaggaagc
     6901 cgccggcaca aatgacaacg ccggcgagcc cgagggctgc gccggcgttg tctgcgtgcg
     6961 gaaaggactc agcgcggcag ggtgtagtcg gcaggcccca tcaggtccat gccgcactcc
     7021 agctggctga tccagtcgag gaaggcggtg atcatcgccg gaccgacgaa gggacgaccg
     7081 tgctgcggca caatcatcga cacatccatc ttgcgcacca tgtcggccca caggcggcag
     7141 accttgttgc tggccatgta gcggcggtgg aaaccttcca tgctctgcag gtgggcggcg
     7201 aaatcgctga ccggctgggc atcctcgacc agcgaggcgc ccatgtcgcc ggagaagagg
     7261 atcttgctca ccggatcgta cagctggaag ttgccgaccg agtgcaggaa gtgcgccggc
     7321 accgccttga gctggcagtt gcccagcggc aggctctggc cacggtcagg cagcgcgatg
     7381 atgcggtcgt aggtggagat gccgtggctg acggccaggt agttggcggt caggtgcggc
     7441 aggaagcggg cccacagccg cgagcagatg accttggcct tggtgtgcag cagccacttg
     7501 tccagggcgg cgatgatgtc cgggtcctgg tgcgaggcga agatgtaggt caggtcctgc
     7561 accggaatgt gcttggacag ctcgagcgac aggggggtgt aggtcaggtc gccacccgga
     7621 tcgagcagca tgtattgctc gttgtcggtg atcaggaact ggttggtctg cacaccttca
     7681 ccggtgacca gatcatcgaa acacaggcac tggtgggtgc cgttatcaaa caggactatc
     7741 ggctcgcgac gcatggctac ctcatattct acgtttgcac cagcttaacg aggccgggtt
     7801 acaagttcac tgacttgcat caagcgcgcc agagccgccg gcctcgtcga cggtacggca
     7861 ctgtacgccg agcgctcacc gcggcgttgc gccggcagtc acagaagcat caccgccacg
     7921 tcaccagtca gacatcccgg cttgccagag tggccatgca cacaacaagg aagcggcccc
     7981 ctgtggctgc ccggaggttc tgcatgtccg ccattgcact gtcctgcgag tccagcccca
     8041 gccgccgcct gcaggccgaa cgcctgcagg gcaccgaatc actgatcgaa gcccaggcct
     8101 tgcgctaccg cgtgttcggc gaggagttcg gcgcccgcct gcaaggtgcc gaacagggcc
     8161 tcgactgcga cggcttcgac gcccactgca gccatctcgg cgtgcgcgac ctcgccagcg
     8221 gcgaactggt cgccaccacc cgcctgctcg accacgccac cgcccgccag ctgggccgat
     8281 tctacagcga ggacgagttc cgcctgcatg gcctgagcaa gctgcaggga ccgattctgg
     8341 agattggccg cacctgcgtc gccgccgaat accgcaacgg cgccaccatt gccgtgctgt
     8401 ggggcgcgct ggcggaaacc ctcaacgagg gcaactatcg ctacctgatg ggctgcgcca
     8461 gcattcccat gcgcgacggc ggcatccagg cccgcgcggt gatgcagcgc ctgcgcgaac
     8521 gccacctctg ccacgaccac ctgcgcgccg agccgcgcca tccgctgccg gccctcgacg
     8581 tgccggacaa cctgactgcc gaactgccgc cgctgctcaa ggcctacatg cgcctggggg
     8641 cgaagatctg cggcgagccg tgctgggatc cggacttcca ggtcgccgac gtgttcatcc
     8701 tgctcaagcg cgacgaactc tgcccgcgct acgcccggca cttcaaggcg gtcggctgat
     8761 gcgccgtctg cgcctgtatg cgcgcctgct gcggctgttc ctggtgctcg gcgaaggcgc
     8821 cctgttcgcc gcctgggtcg agctgcgcga gcgcctcggc ggcgcgccga tggcgctgcg
     8881 ccagcgcctg acctggcgct tcatgcgcca cctgcgcgct gccctgccct tcacggtaca
     8941 gatccacggc cggctgccgg acaccccggt gctgtggctg gccaaccacg tttcctgggt
     9001 cgacatcgcc gttctcggcg ccctgcgccc gttgtcgttc ctcgccaagt ccgaggtcgg
     9061 cgactggccg ctggccggct ggctggcccg ccaagccggc accctgttca ttcgccgcgg
     9121 cgccggcgac agcctgcagc tgggtcgcca gctggccgag caactggccc agcagcgcca
     9181 cctggcgatc ttccccgagg gcaccagctc cgacggcagt gcggtactgc gcttccatcc
     9241 gcgcctgctg gcggcggcgg tggacagcgg cgcggcgctg cagccggtgg cgat
//
LOCUS       DATCUN010000135        21449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_136625, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000135 DATCUN010000000
VERSION     DATCUN010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 21449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21449
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136625"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VIO83_05430"
     CDS             <1..273
                     /locus_tag="VIO83_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY8330750.1"
                     /translation="RIRLAWLLLAGLPAAAIALSRVYLGVHWPTDVIAGALLAGSLCA
                     ASLVLVQRQSPLAAPGPRLWWWLAPGLLLIGLFGWLATDGSALYRY"
     gene            complement(274..978)
                     /locus_tag="VIO83_05435"
     CDS             complement(274..978)
                     /locus_tag="VIO83_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEY8330751.1"
                     /translation="MAAALQAILDHPLFGFGITLGAYQLALALFERTRWVFLQPVLLS
                     MALVVGVLLLCGIDYAEYRASVSMLSVFLGPATVALAVPLFLNLKRIRQLLWPVLLTL
                     ALAGSLATALGIGFAWLFGADHVMQMTVLPKSVTSPIAMLIAEQLGGIAALAAVFVLI
                     VGVIGAIFGPGLLTGIGVHSPAARGMALGITAHAVGTAQALQEGEETGAFSALAMSLM
                     GGATAILLPLVVALVS"
     gene            complement(987..1352)
                     /locus_tag="VIO83_05440"
     CDS             complement(987..1352)
                     /locus_tag="VIO83_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEY8330752.1"
                     /translation="MLLRGITWLVLFQLLGTGLNVLLLPMLPGPIIGMLLLFAFLVWR
                     GEVPAPVGEAGGALLRYLPLLLVPPAVGVMAYAEAIAADFWALAGTLVLSTLLAMLFA
                     GWLMQKLIDRQQRREEGEA"
     gene            1500..2147
                     /locus_tag="VIO83_05445"
     CDS             1500..2147
                     /locus_tag="VIO83_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY8330753.1"
                     /translation="MPHSNSPRNLLIFGASGLTGEHLLDRVLNEPTVQRVLAPSRRPL
                     AAHSHLENPYGDAREVLAKLTGPVDTAFCCLGTTLRQAGSESAFTAIDRDLVIACAQR
                     ARELGARHFLVLSSLGADPQSRVFYSRIKGEMEEALKAQGWPQLTIARPSLLLGQRRE
                     QRLIEQVAAPMARILLGKWRGVPVCLLARALWRLALEERSGVRIVESDELRRLGR"
     gene            2269..2655
                     /locus_tag="VIO83_05450"
     CDS             2269..2655
                     /locus_tag="VIO83_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330754.1"
                     /translation="MTGFTPWKSQDLKGIAVIEAQRCWLSELADTLSAHLHLNSSREA
                     IGECLTQLLSGLLQSIVSEEQAFLELGSPVDAAHRAEHNELCMEVLELIKRHELGDSV
                     GLQLLQRLQDWLALHCAGTPHRAVLH"
     gene            complement(2754..5021)
                     /locus_tag="VIO83_05455"
     CDS             complement(2754..5021)
                     /locus_tag="VIO83_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HEY8330755.1"
                     /translation="MQAAKPLFDYPKYWAECFGPAPFLPMSREEMDLLGWDSCDVIIV
                     TGDAYVDHPSFGMAIIGRLLEAQGFRVGIISQPDWQSKDDFMKLGEPNLFFGVAAGNM
                     DSMINRYTADRKARSDDAYTPGGMAGKRPDRASMVYSQRCREAYKGVPIVLGGIEASL
                     RRIAHYDYWQDKVRHSILMDAKADLLLYGNAERAIIEVAQRLAAGEPVQAISDVRGTA
                     FVRRDTPAGWLELDSTRIDRPGKIDKVINPYVNTQDLEVCAIEQAKGEVDDPNEAKPV
                     ELLPHPRLERDKTVIRLPSFEKVRSDAVLYAHANRVLHLETNPGNARALVQRHGDSDV
                     WLNAPPIPLTTEEMDYVFGLPYARVPHPAYQGEKIPAYEMIRFSVNIMRGCFGGCTFC
                     SITEHEGRIIQNRSEESILREIEEIRDKVPGFTGVISDLGGPTANMYRIACKSREIEA
                     ACRKPSCVYPGICDNLNTDHSALIQLYRDARALPGVKKILIASGLRYDLAVESPEYVR
                     ELVTHHVGGYLKIAPEHTERGPLDKMMKPGIGTFDRFKKMFDKYTKEAGKEQYLIPYF
                     IAAHPGTTDEDMMNLALWLKRNGFRADQVQAFYPSPMATATAMYHSGRNPLRKVTYKS
                     EGVAIVKGERQRRLHKAFLRYHDPSNWPLLREALKSLGRADLIGPGKHQLIPATQPVG
                     EGGYQSARRKNSTPVGSKKVGGAASGKPLLTQHSGLPPRASDGSKPWSKGEEGKAQAF
                     AKGKKKPAGKRPGAR"
     gene            complement(5241..6314)
                     /gene="alr"
                     /locus_tag="VIO83_05460"
     CDS             complement(5241..6314)
                     /gene="alr"
                     /locus_tag="VIO83_05460"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY8330756.1"
                     /translation="MRPLVATIDLSAIRHNYMLAKACAPERLVFAVVKANAYGHGARE
                     AVSALPEADGFIVACLEEAGEVRALHGKARVLLLQGCFEAKEYLMAAQLALDVVVQSR
                     EQAEALLGSALNRPLNIWLKLDSGMHRLGFAADELRAWHARLAAAEQVAELNLISHFA
                     CADLPEHPLNRIQLDCYRSMDDLPFDCRSLANSAAILTRPEAHMDWLRPGIMLYGASP
                     FADRPAAELGLRPAMTLGGSLIAVREVPAGDSVGYGASWVAERPTRIGTISCGYADGY
                     PRSAPAGTPVLVCGQRVPLVGRVSMDMLTVDLSAVPEAGVGSPVELWGAQLPVDEVAA
                     ACGTIGYELLSKVTARVPRRHRY"
     gene            complement(6399..7793)
                     /gene="dnaB"
                     /locus_tag="VIO83_05465"
     CDS             complement(6399..7793)
                     /gene="dnaB"
                     /locus_tag="VIO83_05465"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY8330757.1"
                     /translation="MNEISVPEQYDLETAALKVPPHSIEAEQAVIGGLMLDNNAWERV
                     LDLVSDGDFYRHDHRLIFRAIYKLAERNQPFDVVTLSEQLDKEGQLAQSGGLAYLGEL
                     AKNTPSVANIKAYAQIIRERATLRQLIGISNEIGDSAYNPQGRGADEILDEAERKIFQ
                     IAEARPKSGGPVGLNELLTKAIDRIDTLFNTHEAITGISTGFADLDDKTSGLQPGDLI
                     IVAGRPSMGKTTFAMNLVENAVLRSDKAVMVYSLEMPGESLVMRMLSSLGRIDQTKVR
                     SGRLDDDDWPRLTSAVNLLNERKLFIDDTAGITPSEMRARTRRLVREHGELALIMVDY
                     LQLMQIGGSGGENRTNEISEISRSLKGLAKEFNCPVIALSQLNRSLEQRPNKRPINSD
                     LRESGAIEQDADVIMFVYRDEVYHPETEHKGVAEIIIGKQRNGPIGTVRLAFLGKYTR
                     FENLAPGMYNFDDE"
     gene            complement(7907..8353)
                     /gene="rplI"
                     /locus_tag="VIO83_05470"
     CDS             complement(7907..8353)
                     /gene="rplI"
                     /locus_tag="VIO83_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY8330758.1"
                     /translation="MEIILLEKIANLGNLGDKVNVKAGYGRNYLLPQGKATVASAENV
                     AAFEARRAELEKLAAEKKAYAESRAAQLAELEVTITATAGDEGKLFGSIGTHDIADAL
                     TASGVEVAKSEIRLPNGTIRQVGEYDVAVHLHSDVEATVRVVVVAA"
     gene            complement(8375..9268)
                     /locus_tag="VIO83_05475"
     CDS             complement(8375..9268)
                     /locus_tag="VIO83_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020306867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330759.1"
                     /translation="MRALADFVMRGRLQATLAVVVAAALPLLFWVSAAAGSLVLLRRG
                     LNDALGIVLWALLPTLLWWYFGEPRPLLVLSGSLALALVLRISVSWTRVLLASVLLGV
                     LYGVIIDAVFGEVVKRNATELLELMPQMLGENYRQMTADEKAQIGGLLAPVLTGLMAG
                     LLQLLSLLCVLLGRYWQALLYNPGGFGREFRAVRLPAPMAVSLLVGIFLLPGLSIELA
                     MLTPLCSVPLAFAGVALLHGLVAQQRLARFWLVGLYVSLLLFMQLVYPLLVVLAVVDS
                     LFDFRGRAARDGANGPTDGEG"
     gene            complement(9303..9533)
                     /gene="rpsR"
                     /locus_tag="VIO83_05480"
     CDS             complement(9303..9533)
                     /gene="rpsR"
                     /locus_tag="VIO83_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY8330760.1"
                     /translation="MARFFRRRKFCRFTAEGVLEIDYKDLNTLKAYVSETGKIVPSRI
                     TGTKAKYQRQLATAIKRARYLALLPYTDSHGR"
     gene            complement(9565..9975)
                     /gene="rpsF"
                     /locus_tag="VIO83_05485"
     CDS             complement(9565..9975)
                     /gene="rpsF"
                     /locus_tag="VIO83_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY8330761.1"
                     /translation="MRHYEIVFLVHPDQSEQVGGMVERYTKTIEEDGGKVHRLEDWGR
                     RQLAYAIDNVHKAHYVLMNVECSGAALAELEDNFRYNDAVIRNLVIRRDEAVTEQSEM
                     LKAEESRNERRERRDRSDSAEGAAEGSDGDSADE"
     gene            complement(10140..10892)
                     /gene="rlmB"
                     /locus_tag="VIO83_05490"
     CDS             complement(10140..10892)
                     /gene="rlmB"
                     /locus_tag="VIO83_05490"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY8330762.1"
                     /translation="MSQWERVYGVHAVEALLRHHPKRVKQLWLAEGRQDPRVQALVQL
                     AGEARVPVGQRERRELDEWAEGVHQGVVAEVSPSQVWGENFLDELLARGESVPLLLVL
                     DGVTDPHNLGACLRTADAAGVQAVIVPKDKSATLNATVRKVACGAAEVMPLVAVTNLA
                     RTLEKLQQQGLWVVGTAGEATQEIYDLDLRGPTVLVMGAEGKGMRRLTREHCDYLAKL
                     PMGGSVSSLNVSVATGVCLFEAVRQRRPSVPA"
     gene            complement(10889..13642)
                     /gene="rnr"
                     /locus_tag="VIO83_05495"
     CDS             complement(10889..13642)
                     /gene="rnr"
                     /locus_tag="VIO83_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEY8330763.1"
                     /translation="MADDWQTLDPEAAREAEKYANPIPSRELILAHLAERGAPASRAQ
                     LFEELGLHGEEAEEALRRRLRAMERDGQLIYTRRGAYAPVDKLDLIRGRVSGHRDGFG
                     FLIPDDGSDDLFLSPAQMRLVFDGDRALARVAGLDRRGRREGALVEVLTRAHETLVGR
                     FFEESGVSMVVADNPKVQQQVLVLPGKQGAARHGQFVQVRIDVWPTAFRQAQGEVVEV
                     LGDYMAPGMEIEVAMRSYDLPHEWPEALLKEAKKLKPEVEDKDKEKRVDLRGLPFVTI
                     DGEDARDFDDAVYAEAVKGAGWRLFVAIADVSHYVKVGSALDEEAVKRGNSVYFPERV
                     IPMLPEELSNGLCSLNPLVDRLAMVCEMTVSKSGELTGYQFYEAVIHSHARLTYTKVS
                     QLLEKPNSTEAKYLREQMPHLVPHLRQLYALYKVLLAARQVRGAIDFETQETRIVFGA
                     DRKITEIRPTQRNDAHRLIEECMLCANVATALFLKEHEVPALYRVHDGPPAEKLEKLR
                     SFLGELGLTLNRGKGDPTPKDYLKLLEAVRERPDQHLIQTVMLRSLSQAVYSADNAGH
                     FGLNYEAYTHFTSPIRRYPDLLVHRAIRSVIRSKRATEKVRRVGAASMPKARIYPYDE
                     GRLEQLGVQCSMTERRADEATRDVVNWLKCEYMRERVGERFPGVVTAVTGFGLFVELT
                     DIYVEGLVHVTALPGDYYHFDPVHHRLSGERSGRSFRLGDTVEVLVARVDLDERKIDF
                     ELGDTPQSVQPARKRAGKAEEGRTGAGKAPGRKRGAAREPEPVAAESRSPRGPARKTG
                     RVQALPEVAPALEEQADALLGNVDVHKSRALKKKLLEEAHAPAKPSAKGGKGGKAGAG
                     KGGKDKAAGAGKASAKKAIPRPVRVSGAGKAKPAKKAGAAGSAPAADAPAGRAIRKRK
                     VKE"
     gene            13846..13932
                     /locus_tag="VIO83_05500"
     tRNA            13846..13932
                     /locus_tag="VIO83_05500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13880..13882,aa:Leu,seq:cag)
     gene            14005..14091
                     /locus_tag="VIO83_05505"
     tRNA            14005..14091
                     /locus_tag="VIO83_05505"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14039..14041,aa:Leu,seq:cag)
     gene            complement(14251..15546)
                     /locus_tag="VIO83_05510"
     CDS             complement(14251..15546)
                     /locus_tag="VIO83_05510"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY8330764.1"
                     /translation="MGKNVVVLGTQWGDEGKGKIVDLLTDQAAAVVRYQGGHNAGHTL
                     VIDGEKTVLHLIPSGILRENVQCLIGNGVVVAPDALLKEITKLEEKGVPVRERLRISP
                     ACTLILPYHVALDQAREAARSEGKIGTTGRGIGPAYEDKVARRGLRIADLFNPERFAV
                     KLKELLTYHNFVLENFYKVDPVDYQKTLDEALAYAEILKPMVTDVAARLHELRKAGAY
                     IMFEGAQGSLLDIDHGTYPYVTSSSTTAGGTATGSGFGPLYLDYILGITKAYTTRVGS
                     GPFPTELFDDVGARLASKGHEFGSTTGRARRCGWFDAVILRRAIEINSISGLCLTKLD
                     VLDGLETVKLCVAYKNAAGELVSEAPTDADSYLGLEPVYEEMPGWSESTVGAQSVEQL
                     PANARAYIKRVEELVGAPIDIISTGPDRNETIVLRHPFA"
     gene            complement(15601..16788)
                     /locus_tag="VIO83_05515"
     CDS             complement(15601..16788)
                     /locus_tag="VIO83_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY8330765.1"
                     /translation="MATLDRWLLPDGIEEVLPAEAARIETARRQVLDLFRNWGYELVI
                     TPHIEYLESLLTGAGQDLDLRTFKVTDPLSGRQMGFRADITPQVARLDAHTHRQEGPS
                     RLCYAGSVLHARPGVLSTSRSPIQIGAELYGDASTASDSEVISLMLEVLELAGVAEVH
                     MDLGHVGIYRGLARAAGLSGEAEQQLFDALQRKAIDEVAALTAALPVEQASMLRALAE
                     LCGGREVLAQARAQLAAAPAEVLAALAELEELASLLSARYPTLPLYFDLGELRGYHYH
                     TGAVFAAFVPGVGQAIAQGGRYDDIGAHFGRARPATGFSTDLKALVGLGQAIQAEAPG
                     GVWAPQGGDARLWQAVLELRRSGRRVVQALPGQTQAEARAAGCDSELHLQEGGWQVSP
                     LAS"
     gene            complement(16821..17006)
                     /locus_tag="VIO83_05520"
     CDS             complement(16821..17006)
                     /locus_tag="VIO83_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEY8330766.1"
                     /translation="MWQELGIAFCLLLVLEGILPFLCPRRWREAVVSLARLEDRQLRL
                     IGLGSMLLGTALLYGLR"
     gene            complement(17112..17981)
                     /gene="hflC"
                     /locus_tag="VIO83_05525"
     CDS             complement(17112..17981)
                     /gene="hflC"
                     /locus_tag="VIO83_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEY8330767.1"
                     /translation="MSNKSLIALIAAAVLALIGWNCFYIVLQTEKAVLLQFGRIVQAD
                     VQPGLHVKIPYVNQVRKFDARLLTLDSPTERFLTLEKKAVMVDAFAKWRVADAERFYT
                     ATSGMKQIADERLSRRLESGLRDQFGKRTLHEVVSGERDALMADITQLLDRMARRELG
                     IEVLDVRVKAIDLPKEVNRSVFERMSTEREREAREHRAKGRELAEGIRADADRQRRVL
                     LAEAYRESEELRGQGDAQAAAIYAKAYSQDAEFYSFHRSLQAYRQSFSGKRDMMVLDP
                     KSDFFRYLEQSRP"
     gene            complement(17981..19141)
                     /gene="hflK"
                     /locus_tag="VIO83_05530"
     CDS             complement(17981..19141)
                     /gene="hflK"
                     /locus_tag="VIO83_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEY8330768.1"
                     /translation="MAWNEPGGGNSNDQDPWGGRRGGGRQGPPDLDEALRKLQDNLNS
                     LFGGRKRGGADGGTGKGGGVGLLAIGFGLLAALWLYSAVYVVDEQEQAVILRFGKYYE
                     TVGPGLNLYFPPIDRKFQDNVTRERAYTKQGQMLTEDENIIEVPLTVQYRVSNLQEFV
                     LNVDQPEISLQHATDSALRHVVGSTSMDQVLTEGREQMAGEVKERLQRFLDTYKTGIT
                     VTQVNIQSAAAPREVQEAFDDVIRAREDEQREKNQAEAYANGVIPEARGQAQRIVEDA
                     NGYREEVVARAQGEADRFSRLVAEYRKAPDVTRERLYLETMQEMLSNTSKVLVTGEQG
                     QNNLLYLPLDKMIDSRAPAAGAVAPSAQAGAAEPASRAAVDLQRDLRTRESR"
     gene            complement(19241..20542)
                     /gene="hflX"
                     /locus_tag="VIO83_05535"
     CDS             complement(19241..20542)
                     /gene="hflX"
                     /locus_tag="VIO83_05535"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HEY8330769.1"
                     /translation="MFFERHEGGERAILVHLEGQAPEAREDPDEFLELARSAGAEVVG
                     FVTIPRHQPTARFLIGSGKVEEICEQVKADEADLVIFNHTLTPSQERNLERVFECRVL
                     DRTGLILDIFAQRARTHEGKLQVELAQLDHLSTRLVRGWTHLERQKGGIGLRGPGETQ
                     LETDRRLLRVRIGQIKQRLEKVRSQRELARRGRKRAEVPVVSLVGYTNAGKSTLFNAL
                     TSADVYAANQLFATLDPTLRRLEFDDVGGVILADTVGFIRHLPHKLVEAFRATLEESS
                     NADLLLHVIDAHEPERQLQIEQVYSVLKEIGADQLPTLEVYNKLDLLPDVAPQIQRDD
                     TGRPVRVWLSAREGQGLDLLRQAISELLGSELFVGTLRLPQQLGRLRAQLFALGAVQD
                     EQHDEEGAILLKVRLQRVELNKLVSREGWKSEDFLAQHTLQ"
     gene            complement(20558..20806)
                     /gene="hfq"
                     /locus_tag="VIO83_05540"
     CDS             complement(20558..20806)
                     /gene="hfq"
                     /locus_tag="VIO83_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY8330770.1"
                     /translation="MSKGHSLQDPYLNTLRKERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVRLPSSEQGESGSDE"
     gene            complement(20913..>21449)
                     /locus_tag="VIO83_05545"
     CDS             complement(20913..>21449)
                     /locus_tag="VIO83_05545"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY8330771.1"
                     /translation="RVDPESAARIHPNDPQRLIRALEVYRVSGLSMSEHRQRQVLQNL
                     HAGTPQGQALPYTVAQLVIAPAQRQVLHERIARRFNLMLEQEFIAEVEALHARGDLHP
                     GLPSIRSVGYRQVWEYIEGRLTRDEMTERGIIATRQLAKRQFTWLRSWSQLHWLDSLA
                     CDNLARTLKYLQTIAIKD"
BASE COUNT         3703 a         7286 c         6766 g         3694 t
ORIGIN      
        1 cgcatccgcc tggcctggct gctgctggcc ggcctgccgg ccgccgccat cgccctgtcg
       61 cgggtgtatc tcggcgtgca ctggccgacc gacgtgatcg ccggcgccct gctggctggc
      121 agcctctgcg ccgccagcct ggtcctggtg cagcggcaaa gtccgctggc cgctcccggc
      181 ccgcgcctgt ggtggtggct ggcgcccggc ctgctgctca tcggcctgtt cggctggctg
      241 gcgaccgacg gcagcgcgct gtaccgctac tgatcaggac accagcgcca ccaccagcgg
      301 caacaggatc gccgtggcac cgcccatcag gctcatcgcc agcgccgaaa aggcgccggt
      361 ttcctcgcct tcctgcagag cctgggcggt acccaccgca tgggcggtga tccccagcgc
      421 cataccgcgc gccgccgggc tgtgcacgcc gatgccggtc agcaatccgg gaccgaagat
      481 tgccccgatc accccgacga tcagcacgaa caccgccgcc agcgcggcga tgccgccgag
      541 ctgctcggcg atcagcatgg cgatcggcga ggtcaccgac ttgggcagca cggtcatctg
      601 catgacgtgg tcggcgccga acagccaggc gaaaccgatg cccagcgcgg tggccaggct
      661 gccggccagc gccagggtca gcaggaccgg ccagagcagc tggcggatgc gcttgagatt
      721 gaggaacagc ggcaccgcca gcgccaccgt ggccggaccg aggaataccg agagcatgct
      781 gacgctggcg cggtactcgg cgtagtcgat gccgcacagc agcagcacgc cgaccaccag
      841 cgccatcgac agcagcaccg gctgcaggaa tacccagcgg gtgcgctcga acagggccag
      901 ggccagttgg taggcaccga gggtgatgcc gaagccgaac agcggatgat ccaggatcgc
      961 ctgcagggcg gcagccagag tgccactcat gcctcaccct cctcgcgacg ctgctgacgg
     1021 tcgatcagct tctgcatcag ccagccggca aacagcatgg ccagcagcgt cgacagcacc
     1081 agggtgccgg ccagcgccca gaagtcggcg gcgatcgcct cggcataggc catcaccccc
     1141 accgccggcg gcaccagcag cagcggcagg tagcgcagca acgcaccgcc tgcctcgccg
     1201 accggcgccg gcacctcgcc acgccagacc aggaaggcga acagcagcaa catgccgatg
     1261 atcggccccg gcaacatcgg caacaacagc acgttcaacc ccgtgcccag cagctggaac
     1321 agcaccagcc aggtgattcc ccgcaacagc atcgcaaccc ctcccaagaa cttgatcgca
     1381 ttatagggac gagcccagca attacacccc agtccgcttg accaccgcgc gtcgcacatg
     1441 ctgatctaga cttgtaacca ccggcgccgc aagcccggcc attcaggatc acagcagcca
     1501 tgccgcactc caacagcccc cgcaatctgc ttatcttcgg cgccagcggc ctgactggcg
     1561 agcatctgct cgatcgcgta ctcaacgaac ccaccgtcca gcgtgtgctg gcgcccagtc
     1621 gacgccccct ggcggcccac tcgcacctgg aaaaccccta cggcgatgcc cgggaggtcc
     1681 tggcgaaact gacgggcccc gtggatacgg ccttctgctg cctcggcacg accctgcgcc
     1741 aggcgggctc cgaaagtgcc ttcaccgcca tcgatcgcga cctggtgatc gcctgcgccc
     1801 aacgcgcccg cgaactgggc gcgcgccact tcttggtgct cagctcgctg ggcgccgatc
     1861 cgcagtcgcg ggtgttctac agccggatca agggcgagat ggaagaagcc ctcaaggcgc
     1921 agggctggcc gcaactgacc atcgcccgcc cttccctgct gctcggccag cgccgcgagc
     1981 agcgcctgat cgaacaggtc gccgcgccca tggcgcgtat tttgcttgga aaatggcgcg
     2041 gggttccagt atgcctactg gctcgcgcgc tctggcgtct ggcgctggaa gaacgcagcg
     2101 gagtccgcat cgtcgagtcg gacgaactgc gtcgtttggg gcgttgagac acgtccatca
     2161 ccgggctgta acaatccatt gttaccgtgc cgcgcatcac gaatttgacc aaccaagccg
     2221 cgagcacagc ggcgcatcca tgctggatgc ttgaagaagg gacacgctat gactggattc
     2281 actccctgga aatcccaaga cctgaagggc atcgccgtca tcgaggcaca gcgctgctgg
     2341 ctcagcgagc tggcggacac cctcagcgcc cacctgcatc tgaacagctc ccgggaggcg
     2401 atcggcgagt gcctgaccca actgctgagc ggcctgctgc aatccatcgt cagcgaggag
     2461 caagcctttc tcgaactggg cagcccggtc gacgccgcgc atcgcgccga acataacgag
     2521 ctgtgcatgg aagtgctcga actgatcaag cgccacgagc tgggcgacag cgttggcctg
     2581 caactgctgc agcgcctgca ggactggctg gccctgcact gcgcaggcac gccgcaccgc
     2641 gccgtcctgc actgaggcac ccgtcggtgc cgcccgctgc tggcggcacc gattctgcgt
     2701 aatttctttc cgcgctgacc accgagcccg cggcgcgggc tcggtggctc tgctcagcgt
     2761 gcgccgggac gcttgccggc cggcttcttc ttacccttgg cgaacgcctg cgccttgcct
     2821 tcctcgccct tgctccacgg cttgctgcca tcgctggcgc gcggcggcag accgctgtgc
     2881 tgggtcagca gcggcttgcc tgaggcggcc ccaccgactt tcttgctgcc caccggggtg
     2941 gaattcttgc gccgcgcgct ctggtagccg ccctctccca ccggctgggt ggccgggatc
     3001 aactggtgct tgccggggcc gatcaggtcg gcgcggccca ggctcttcag cgcctcgcgc
     3061 agcagcggcc agttgctcgg gtcgtggtag cgcaagaagg ccttgtgcag gcggcgctgg
     3121 cgctcgccct tgacgatggc cacgccctcg ctcttgtagg tgaccttacg cagcgggttg
     3181 cgccccgagt ggtacatggc ggtggcggtg gccatcggcg acgggtagaa cgcctgcacc
     3241 tggtcggcgc gaaagccgtt gcgcttgagc cacagggcga ggttcatcat gtcctcgtcg
     3301 gtggtgccgg ggtgggcggc gatgaagtac gggatcaggt actgctcctt gcccgcctcc
     3361 ttggtgtact tgtcgaacat cttcttgaag cggtcgaagg taccgatccc cggcttcatc
     3421 atcttgtcca gcgggccgcg ctcggtatgc tccggagcga tcttcagata gccgccgaca
     3481 tggtgggtga ccagctcccg gacgtactcc ggcgactcca cggcgaggtc gtagcgcagg
     3541 ccggaggcga tgaggatctt cttcaccccg ggcaacgcgc gggcgtcacg gtacagctgg
     3601 atcagcgccg aatggtcggt attcaggttg tcgcagatgc ccggatacac gcacgacggc
     3661 ttgcggcagg cagcctcgat ctcgcggctc ttgcaggcga tgcggtacat gttggcggtc
     3721 ggcccgccga ggtcggaaat caccccggtg aagcccggca ccttgtcgcg gatctcctcg
     3781 atctcgcgca ggatcgactc ctccgagcgg ttctggatga tccgcccctc gtgctcggtg
     3841 atcgagcaga aggtgcagcc gccgaagcag ccacgcatga tgttgaccga gaaacggatc
     3901 atctcgtagg ccgggatctt ctcgccctgg taggccgggt gcggcacgcg ggcgtaaggc
     3961 agaccgaata cgtagtccat ctcctcggtg gtcagcggga tcggcggcgc attcagccac
     4021 acgtcgctgt cgccgtggcg ctgcaccagg gcgcgggcgt tgcccggatt ggtctccagg
     4081 tgcagcacgc ggttagcgtg ggcgtagagg accgcgtcgc tgcgcacctt ctcgaacgac
     4141 ggcaggcgga tcaccgtctt gtcccgctcc aggcgcggat gcggcagcaa ctcgaccggc
     4201 ttggcctcgt tggggtcgtc cacctcgccc ttggcctgct cgatggcaca gacctcgaga
     4261 tcctgggtgt tgacgtaggg attgatgacc ttgtcgatct tgccgggacg gtcgatgcgc
     4321 gtggaatcga gctcgagcca gcccgcgggg gtgtcgcggc gcacgaaggc ggtaccgcgc
     4381 acatcgctga tcgcctgcac cggctcgccg gcggccaggc gctgggccac ttcgatgatg
     4441 gcgcgctcgg cgttgccgta cagcagcagg tcggccttgg cgtccatcag gatcgagtgg
     4501 cgcaccttgt cctgccagta gtcgtagtgg gcgatgcggc gcagcgaggc ctcgatgccg
     4561 ccgagcacga tcggcacgcc cttgtaggct tcgcggcagc gctggctgta gaccatgctt
     4621 gcgcggtccg ggcgcttgcc ggccataccg cccggcgtat aggcatcatc cgagcgcgcc
     4681 ttgcggtcgg cggtatagcg gttgatcatc gagtccatgt tgcccgccgc caccccaaag
     4741 aacaggttcg gctcgccgag cttcatgaag tcgtccttcg actgccagtc cggctgactg
     4801 atgatgccga cgcggaaacc ctgggcctcg agcaggcgac cgatgatcgc catgccgaac
     4861 gacgggtggt cgacgtaggc atcgccggtg acgatgatca cgtcgcagga atcccagcca
     4921 agcagatcca tctcttcccg actcatcggc aggaagggtg cgggtccgaa acattcggcc
     4981 cagtacttgg gatagtcgaa cagcggcttg gcggcttgca tggcgatgac caggaaatct
     5041 cgggaggggg ttggcgcggg cgcggaggat agcacaaaaa gtgaacaagt ccgacgcttg
     5101 gggtcgggat tggcggcgcg gctgtcgagg ctgcgcagtg ggacgctcag cgccccatcc
     5161 gccacgcaac ggacgcgaag cgtccgcgtc cgggctccca cgcaggagcg tggcagccat
     5221 caaatcaatc gtttctcggc tcagtaacgg tggcggcgcg gcacccgtgc ggtgaccttg
     5281 ctgagcagct cgtagccgat ggtgccacag gcggcagcca cctcgtccac cggcagctgg
     5341 gcgccccaca gctccaccgg cgagccgacc ccggcctcgg gcaccgccga gagatccacc
     5401 gtcagcatat ccatggaaac gcgaccgacc agcggcacgc gctggccgca gaccagcacc
     5461 ggcgtgcccg ccggcgcgct gcgcgggtag ccgtcggcat agccgcagct gatggtgccg
     5521 atgcgcgtcg gccgctcggc gacccaactg gcgccgtagc cgacgctgtc gccggccggc
     5581 acttcgcgca cggcgatcag gctgccaccg agggtcatcg ccgggcgcag acccagctcg
     5641 gcggccggac gatcggcgaa cgggctggcg ccgtagagca tgatgcccgg tcgcaaccag
     5701 tccatgtgcg cctcgggacg ggtgaggatg gccgccgagt tggccagcga gcggcagtcg
     5761 aacggcaggt cgtccatgct gcggtagcag tccagctgaa tccggttgag cgggtgttcg
     5821 ggcaggtcgg cacaggcgaa gtgactgatc aggttaagct cggccacctg ctcggcagcg
     5881 gccaggcgcg catgccaggc gcgcagctcg tcagccgcga atcccaggcg atgcatgccg
     5941 gaatccagct tgagccagat gttcagcggg cgattcagcg cgctgccgag cagcgcctcg
     6001 gcctgctcgc gactctgcac caccacgtcc agcgccagct gcgcggccat caggtactcc
     6061 ttcgcctcga agcagccctg cagcagcagc acgcgcgcct tgccgtgcag ggcgcgcacc
     6121 tcgccggcct cctccaggca ggcgacgatg aagccgtcgg cttccggcag ggcactgacc
     6181 gcctcgcgtg cgccatggcc gtaggcattg gccttgacca ccgcaaagac cagccgctcc
     6241 ggtgcacagg ccttggccag catgtagttg tggcggatgg cggacagatc gatggtggca
     6301 accagcgggc gcatgacggt tcgacctcga aacggaaaac gggcgcccat cttacccgct
     6361 gggcgcccgt tttccttgat ccagcgatgg attcgcggtc attcgtcgtc gaagttgtac
     6421 atccccggcg ccaggttctc gaagcgcgtg tacttgccga ggaaggccag ccgcacggtg
     6481 ccgatcggac cgttccgctg cttgccgatg atgatctcgg cgaccccctt gtgctcggtt
     6541 tccggatggt agacctcgtc gcggtagacg aacatgatca cgtcggcgtc ctgctcgatg
     6601 gcgcccgact cgcgcaggtc ggagttgatc ggtcgcttgt tcggccgctg ctccagcgag
     6661 cggttgagct gcgacagggc gatcaccgga cagttgaact ccttggccag gcccttcagc
     6721 gagcgggaga tctcggaaat ctcgttggta cggttctccc ctccagaacc gccgatctgc
     6781 atcagctgca gatagtcgac catgatcagc gccagctcgc catgctcgcg caccaggcgg
     6841 cgggtgcgcg cgcgcatttc cgacggggtg atgccggcgg tgtcgtcgat gaacagcttg
     6901 cgctcgttga gcaggttgac cgccgaggtc agccgcggcc agtcgtcgtc gtcgaggcga
     6961 ccggaacgca ccttggtctg gtcgatgcgc ccgagcgagg acagcatacg catcaccagc
     7021 gattcgccag gcatctccag cgagtagacc atcaccgcct tgtcgctgcg cagcacggcg
     7081 ttctccacca ggttcatggc gaaggtggtc ttgcccatcg acgggcggcc ggcgacgatg
     7141 atcaggtcgc caggctgcag gccgctggtc ttgtcgtcca ggtcggcgaa gccggtggag
     7201 atgccggtga tcgcctcatg ggtgttgaac agcgtgtcga tgcggtcgat ggccttggtc
     7261 agcagctcgt tgaggcccac cgggccgcca ctcttcggcc gcgcctcggc gatctggaag
     7321 atcttgcgct cggcctcgtc gagaatctcg tcggcgccac gcccctgcgg gttgtaggca
     7381 ctgtcgccga tctcgttgct gatgccgatc agctgccgca gggtggcgcg ctcgcggatg
     7441 atctgcgcgt aggccttgat gttggccacc gacggcgtgt tcttggccag ctcgccgaga
     7501 taggccaggc cgccggactg ggccagctgg ccttccttgt ccagctgctc ggacagggtg
     7561 accacgtcga acggctggtt gcgctcggca agcttgtaga tggcccggaa gatcagccgg
     7621 tggtcatgcc ggtagaagtc gccgtcggac accagatcca gcacccgctc ccaggcgttg
     7681 ttgtccagca tcaggccacc gatcacggcc tgctcggcct cgatggaatg cggcggcacc
     7741 ttgagggcgg cggtttccag atcgtactgt tcggggacgc tgatttcgtt catgggctcg
     7801 cgatgttctg tgctgcagaa agacaaaggg cacgcctgcg aagacgcagg tcgtgcccga
     7861 tgttaaccgc ccggcatcat cgatgcacgg gcgcttgggg gatgcctcag gcagccacca
     7921 ccaccacgcg aacggtggct tcgacgtcgc tgtgcaggtg cacggccaca tcgtactcgc
     7981 cgacctgacg gatggtaccg ttgggcaggc ggatttcgct cttggccact tccacgccgg
     8041 aggcggtcag ggcgtcggcg atgtcgtggg taccgatcga accgaacagc ttgccttcat
     8101 cgccagcggt ggcggtgatg gtgacttcca gttcagccag ctgggcagcg cgggattcgg
     8161 cgtaggcctt cttctcggca gccagctttt ccagctcggc acgacgggct tcgaacgccg
     8221 caacgttctc ggcgctggcc acggtggcct tgccctgcgg cagcaggtag ttgcgaccgt
     8281 aaccggcctt gacgttaacc ttgtcgccca ggttgcccag gttggcgatt ttttccagca
     8341 ggatgatttc catttggctt taacctctta acttttaacc ttcaccgtcc gtgggcccgt
     8401 tcgcgccatc gcgcgccgcg cgcccacgga aatcaaacag actgtcgaca acggccaaca
     8461 cgaccaacaa cggatacacc agctgcatga acaacagcag cgagacatac aaacccacca
     8521 gccagaagcg cgccaggcgc tgctgcgcca ccagcccgtg cagcagagcc acgccggcaa
     8581 acgccagcgg cacactgcac agcggtgtca gcatggccag ctcgatgctc agtcccggca
     8641 gcaggaaaat tcccaccagc aaggacaccg ccatcggtgc cggcaggcgg acggcgcgaa
     8701 actcgcgacc gaaaccgccc gggttgtaca gcagcgcctg ccagtaacgg cccagcagca
     8761 cacagagcag gctgagcagt tgcagcaggc cggccatcag gccggtcagc accggcgcca
     8821 acagcccgcc gatttgcgcc ttctcgtctg ccgtcatctg ccggtagttc tcacccagca
     8881 tctgcggcat cagctcgagc agctcggtag cgttacgctt gaccacctcg ccgaacacgg
     8941 catcgatgat cacgccatac agcacgccga gcagcacgct ggccagcagg acccgcgtcc
     9001 aggagacgct gatccgcagc accagcgcca acgccagact gccggacaac accagcagcg
     9061 gccgcggttc gccgaagtac caccacagca aggtcggcaa cagcgcccag agaacgatcc
     9121 ccagggcatc gttcagacca cgccgcagca gaaccagcga gcccgccgcg gcgctcaccc
     9181 agaacagcag gggcagcgcc gccgcaacga ccaccgcaag ggtggcctgc aggcgtccgc
     9241 gcatgacgaa gtcagctagt gcccgcatgt attacatccg ttgacctgtc gacgtccggg
     9301 tctcaacgac cgtggctgtc ggtgtagggc agcagggcca ggtagcgggc gcgcttgatc
     9361 gcggtagcca gctgacgctg atatttggct ttggtgccgg tgatacggct cggaacgatc
     9421 ttgccggttt cggagacgta ggccttcagg gtgttgagat ccttgtaatc gatctccaga
     9481 acgccttcag cggtgaaacg gcagaactta cgacgacgga aaaaacgtgc catgaaagtg
     9541 gctcctcaat actggttacg aagattactc gtcggcgctg tcgccgtcgc tgccttcagc
     9601 ggcgccttcg gcgctgtcgc tgcggtcacg acgctcgcgg cgctcgttgc ggctttcttc
     9661 ggccttgagc atctcggatt gttcggtcac ggcttcgtcg cgacggatca ccaggttgcg
     9721 gatcacggcg tcgttgtagc ggaagttgtc ttccagctcg gccagggcag caccgctgca
     9781 ctcgacgttc atcagaacgt agtgagcctt gtgcacgttg tcgatggcgt aagccagctg
     9841 acgacggccc cagtcttcca ggcggtgaac cttgccgccg tcttcttcga tggtcttggt
     9901 gtaacgctcg accataccgc caacctgctc actctgatcc gggtgaacca ggaagacgat
     9961 ttcgtaatga cgcataaatg ctccttacgg ttatgcagcc tgccgccagg gcggtcaggc
    10021 aaggagtgaa tatttcgtgt gtgcttgccg ggcgcgggcg caaatacgcc tgccgcgacg
    10081 gcaaggggtg gcattgtacg gaggggaccg ctgccccgca agcaatgcgg ggcagcgatt
    10141 catgccggca cggacgggcg gcgctggcga accgcctcga acaggcagac gccggtagcc
    10201 accgagacat tgagactgct gacgctgcca cccatcggca gcttggccag gtagtcgcag
    10261 tgctcgcggg tcaggcggcg catgcccttg ccctcggcgc ccatcaccag cacggtcggc
    10321 ccgcgcaggt cgagatcgta gatctcctgg gtcgcctcgc cagcggtgcc gaccacccac
    10381 agcccctgct gctgcagctt ctccagggta cgcgccaggt tggtcaccgc caccagcggc
    10441 atcacttccg ccgcaccgca ggcaaccttg cgcacggtgg cattgagggt cgccgacttg
    10501 tccttgggca cgatcaccgc ctgcaccccg gctgcatcgg cggtgcgcag gcaggcgccg
    10561 aggttgtgcg gatcggtcac cccgtcgagc accagcagca gcggcacaga ctcgccgcgc
    10621 gccagcaact cgtcgaggaa gttctcgccc cacacctgac tcggactgac ctcggcgacc
    10681 acgccctggt gcacgccctc ggcccactca tccagctcgc gccgctcacg ctggccgacc
    10741 ggcacccgcg cctcaccggc cagctgcacc agcgcctgca cccgcggatc ctggcgcccc
    10801 tcggccaacc acaactgctt gacccgcttg gggtgatggc gcagcaatgc ctctacggca
    10861 tgcacgccgt agactcgctc ccactgactc attctttgac cttgcgttta cgtatggctc
    10921 gccccgccgg cgcatccgcc gcgggcgcac tgccggctgc gccggccttc ttggctggct
    10981 tggccttgcc cgcgccggaa accctgaccg gccggggaat ggccttcttc gccgaagcct
    11041 tcccggcacc ggccgcctta tccttgccgc ccttgcctgc acccgccttg ccgcccttgc
    11101 cccccttggc ggacggcttg gccggcgcat gggcctcctc cagcagtttc ttcttcagcg
    11161 cccgactctt atgcacatcg acattgccca gcagcgcatc ggcctgctcc tccagcgccg
    11221 gtgccacttc cggcagggcc tggacccggc cggtcttgcg tgccgggcca cgcggactgc
    11281 gcgactcggc cgctaccggc tcgggttccc gcgcggcgcc acgcttgcgc cctggcgcct
    11341 tgccggcacc cgtacgcccc tcttcagcct tgcccgcacg cttgcgcgcc ggctgtacgg
    11401 actgcggggt gtcgccgagt tcgaagtcga tcttgcgttc gtcgagatcg acccgtgcca
    11461 ccagcacctc gacggtgtcg ccgagacgga agctgcgccc ggaacgctcg ccactcaggc
    11521 ggtgatggac cggatcgaag tggtagtagt cgcccggcag ggcagtgaca tgcaccagac
    11581 cctccacata gatgtcggtc agctcaacga acaggccaaa gccggtgacc gcggtgacca
    11641 cacccgggaa acgctcgccg acccgctcgc gcatgtattc gcacttcagc cagttgacca
    11701 catcacgggt cgcctcgtcg gcgcgccgct cggtcatcga gcactgcacg ccgagctgct
    11761 ccagacgccc ctcgtcgtac ggatagatgc gcgccttggg catgctcgcc gcaccgacgc
    11821 gccgcacctt ttcggtggcg cgcttggagc ggatcacgct gcggatcgcc cgatgcacca
    11881 gcagatccgg ataacgacgg atcggcgagg tgaagtgggt gtaggcctcg tagttgagac
    11941 cgaagtgacc ggcattgtcg gcgctgtaca ccgcctggct cagcgagcgc agcatcacgg
    12001 tctggatcag gtgctgatcc gggcgctcac gcaccgcctc gagcagcttc aggtagtcct
    12061 tgggcgtcgg atcgcccttg ccgcgattca gagtcaagcc cagctcaccg aggaaactgc
    12121 gcagcttctc caacttttcc gcgggcggac cgtcgtgcac gcggtacagc gccggcacct
    12181 cgtgctcctt gaggaacagc gccgtggcga cgttggcgca gagcatgcac tcctcgatca
    12241 gccggtgcgc atcgttgcgc tgggtcgggc ggatctcggt gatcttgcgg tcggcgccga
    12301 agacgatgcg cgtttcctgg gtctcgaagt caatggcgcc gcgcacctgg cgcgccgcca
    12361 gcagcacctt gtacagcgca tacagctgac gcaggtgcgg caccagatgc ggcatctgct
    12421 cgcgcaggta cttggcctcg gtggagttgg gcttctccag caactgactg accttggtgt
    12481 aggtcagacg cgcatgggag tggatcaccg cctcatagaa ctggtagccg gtcagctcgc
    12541 cgctcttcga cacggtcatc tcgcagacca tggccaggcg atcgaccagc ggattcagcg
    12601 agcacaggcc gttggacagc tcttccggca gcatcgggat cacccgctcg gggaaataca
    12661 ccgagttgcc gcgcttgacc gcctcctcgt ccagcgccga gcccaccttc acataatggg
    12721 agacgtcggc aatggcgacg aacagacgcc agccggcgcc cttgaccgcc tcggcgtaga
    12781 ccgcgtcgtc gaagtcgcgg gcgtcctcgc catcgatggt gacgaagggc agcccgcgca
    12841 gatcgacgcg cttctccttg tccttatcct ccacttcggg cttgagcttc ttcgcctcct
    12901 tgagcagggc ctccggccac tcgtgcggca ggtcgtagct gcgcatggca acctcgatct
    12961 ccatgcccgg cgccatatag tcgccgagca cctcgaccac ctcgccctgg gcctggcgga
    13021 acgccgtcgg ccacacatcg atgcgcacct ggacgaactg gccatggcgc gccgcgccct
    13081 gcttgcccgg caggaccagc acctgctgct ggaccttcgg gttgtcggcc accaccatgg
    13141 aaacgccgct ttcctcgaag aagcgaccga ccagggtttc gtgggcgcgc gtaagcactt
    13201 cgaccagcgc gccttcgcgc cggccgcggc ggtcgagacc ggcaacccgc gccagcgcgc
    13261 ggtcgccgtc aaataccaga cgcatctgtg ccggactgag gaacagatcg tcgctgccgt
    13321 cgtcaggaat caggaagccg aagccgtcgc ggtggccgct gacgcgcccg cggatcaggt
    13381 ccagcttgtc caccggggca taggcgccgc ggcgggtgta gatcagttga ccatcgcgct
    13441 ccatggcgcg cagtcggcgg cgcaaggcct cctcggcctc ctcgccatgc agcccgagct
    13501 cctcgaacag ctgcgcacgg gaagccggcg caccgcgctc ggccaagtgc gcgaggatca
    13561 gctcacgact gggaatgggg tttgcgtatt tttccgcctc gcgggcggcc tcgggatcga
    13621 gggtttgcca atcatcggcc atatgtccgg ttttacctgt ggttgtcttg cttgtggtca
    13681 tgcctatatt gaagcgcgaa agcggcgccg agcgcagcct tgtcggcaca ataaaatttt
    13741 ttcatatcag gggtttacaa gctcccccag cccccgtata gtttgcgccc acagcagcag
    13801 tgaacgctgt aacgatagag cagatgagca atcatcgcac tattagccca ggtggcggaa
    13861 ttggtagacg cgctggtttc aggtatcagt ggtggcaaca ccgtggaagt tcgagtcttc
    13921 tcctgggcac caattctaaa cattcgcttc ggcgagtgcg taaaagaagc agtcagcttc
    13981 gccgtgtaac ggcaaaaatg gcacgcccag atggcggaat tggtagacgc gctggtttca
    14041 ggtatcagtg gtggcaacac cgtggaagtt cgagtcttct tctgggcacc aattcgaaaa
    14101 aaccggatct cacgatccgg ttttttttcg cctgaagaaa agcatcccat cctcccccgc
    14161 cccagaacaa agcctgccgg ctgccatcgc gcactttttt ccaggcaaaa aaagaccgcc
    14221 cgcaggcggt ccttttcatg cagccagcga tcaggcgaag ggatgacgca ggacgatggt
    14281 ttcgttgcga tccggaccgg tggaaatgat gtcgatcggc gcacccacca gctcttccac
    14341 gcgcttgatg taggcacggg cgttggccgg cagctgttcg acggactgcg cacccacggt
    14401 ggattcgctc cagcccggca tttcctcgta gaccggctcg agacccagat agctgtccgc
    14461 gtcggtcggc gcttcgctca ccagctcgcc ggcggcgttc ttgtaggcca cgcacagctt
    14521 gacggtttcg aggccatcga gcacatccag cttggtcagg cacaaaccgg agatcgagtt
    14581 gatctcgatg gcgcgacgca ggatcaccgc atcgaaccag ccgcagcgac gggcccgccc
    14641 ggtggtggaa ccgaactcgt gacccttgct cgccaggcgc gcaccgacgt cgtcgaacag
    14701 ctcggtgggg aacggaccgg agccgacgcg cgtggtgtag gccttggtga tgccgaggat
    14761 gtagtccagg tacagcggac cgaaaccgga accggtggcg gtaccgccgg cggtggtgct
    14821 ggagctggtc acgtaggggt aggtgccgtg atcgatatcc agcagggagc cttgggcgcc
    14881 ctcgaacatg atgtaggcac ccgccttgcg cagctcgtgc aggcgggccg ccacgtcggt
    14941 gaccatcggc ttgaggatct cggcgtaggc cagcgcctcg tccagggtct tctggtagtc
    15001 gaccggatcg accttgtaga agttctccag aacgaagttg tgataggtca gcagctcctt
    15061 gagcttgacc gcaaaacgct ccggattgaa caggtcggcg atgcgcaggc cgcgacgcgc
    15121 caccttgtcc tcgtacgccg ggccgatgcc gcgaccggtg gtgccgatct tgccttcgga
    15181 gcgggccgct tcgcgggcct ggtccagggc cacgtgatac ggcaggatca gggtacaggc
    15241 cgggctgatg cgcaggcgct cgcgcaccgg cacacccttc tcttccagct tggtgatttc
    15301 cttgagcagc gcatcgggcg ccaccaccac accgttgccg atcaggcact gcacgttctc
    15361 gcgcaggatg ccggacggaa tcaggtgcag cacggtcttc tcgccatcga tcaccagggt
    15421 gtgaccggca ttgtggccgc cctgataacg caccacggca gcagcctggt cggtcagcag
    15481 gtcgacgatc ttgcccttgc cctcatcacc ccactgggtg cccaggacga cgacattctt
    15541 acccataaca cttgtcccct cgtgaaaggt cggtaccggc gcgcacgccg aagaaagtac
    15601 tcaggaggcc agcggcgaga cctgccagcc accctcctgc aaatgcaatt cgctgtcgca
    15661 gcccgcggca cgggcctctg cctgggtctg cccgggcagt gcctgcacca cccgccgccc
    15721 gctgcgtcgc agctcgagca ccgcctgcca gagccgcgca tcgccgccct gcggtgccca
    15781 gacgccgccc ggagcttcgg cctgaatggc ctgccccaga ccgaccagcg ccttcagatc
    15841 ggtggaaaaa ccggtggccg gtcgcgcgcg gccgaaatgg gcgccgatgt cgtcgtagcg
    15901 tccgccctgg gcaatcgcct gaccgacgcc cggcacgaag gccgcaaaca ccgcaccagt
    15961 gtgatagtga tagccgcgca gctcgcccag gtcgaaatac agcggcagcg tcggatagcg
    16021 cgcgctcaag aggctggcca gctcttccag ctcggccagc gcagccagca cctcagccgg
    16081 cgcagcggcc agttgcgcgc gcgcctgcgc cagcacttca cgtccgccgc acagctcggc
    16141 cagagcgcgc agcatggacg cctgctcgac cggcagagcc gcggtcaatg ccgccacctc
    16201 gtcgatggcc ttgcgctgca gggcatcgaa caactgctgc tcggcttcgc cggacaggcc
    16261 ggcggcacgc gccaagccgc gatagatgcc aacatgaccg agatccatgt gcacctcggc
    16321 gacacccgcc agctccagca cctcgagcat caggctgatg acctcgctat cactggccgt
    16381 gctggcatcg ccatacagct cggcaccgat ctggatcggg ctgcgcgaag tggacagcac
    16441 acccggacgc gcatgcagca cactgccggc atagcacagg cggctcggcc cttcctggcg
    16501 atgggtatgc gcatcgagac gcgccacctg cggcgtgatg tcggcacgga agcccatctg
    16561 ccggccggac aacggatcgg tgaccttgaa ggtccgcaga tcgagatcct gacccgcgcc
    16621 cgtgagcagg gactccaggt attcgatatg cggggtaatg accagctcgt agccccagtt
    16681 gcggaacaga tccagcacct gacggcgagc cgtctctatg cgggccgcct ctgccggcag
    16741 cacctcttcg attccatccg gcagcagcca gcggtccagc gttgccatat tgtctctccc
    16801 cgttcagccg ggcgtggtat tcaacgaagc ccgtacaaca gggccgttcc cagcagcatg
    16861 ctgcccagtc cgatcagtcg cagctggcga tcctccagcc gggccagact gacgacagcc
    16921 tcgcgccagc gccgcggaca gaggaacggc agaatgcctt ccagtaccag cagcagacaa
    16981 aaagcgatgc ccagttcttg ccacatggtt cccgcagacg caaaaaagcc gggaaatccc
    17041 ggctgattca taataacacg ttttccccga ggtcaccccg gcagccgcgc aacggccgcc
    17101 gggtggacgc ctcagggcct ggactgctcc agatagcgga agaagtcgct cttcggatcg
    17161 agcaccatca tgtcgcgctt gccggaaaag ctctgccgat aggcctgcag actgcggtgg
    17221 aaggagtaga actccgcatc ctggctgtag gccttggcgt agatcgccgc cgcctgggca
    17281 tcaccctggc cgcgcagctc ttccgactcg cgataagcct cggccagcag cacgcgacgc
    17341 tggcggtcgg cgtcggcacg aatgccttcg gccagttcgc gacccttggc gcggtgctcg
    17401 cgggcctcgc gctcgcgctc ggtactcatg cgctcgaaca cgctgcgatt gacctccttg
    17461 ggcaggtcga tggccttgac tcgcacgtca agcacctcaa tgcccagctc gcgacgcgcc
    17521 atgcgatcca gcaactgggt gatatcggcc atcagcgcat cgcgctcgcc ggacaccact
    17581 tcatgcaggg tgcgcttgcc gaactggtcg cgcagaccgg actccagacg ccgcgacaga
    17641 cgctcgtcgg caatctgctt catgcccgag gtcgccgtgt agaagcgctc ggcatcggcc
    17701 acccgccact tggcgaaggc gtcgaccatc actgccttct tctccagagt gaggaagcgc
    17761 tcggtcggcg agtccagcgt cagcaggcgc gcatcgaact tgcgcacctg attgacgtaa
    17821 gggatcttca catgcagacc cggctgcacg tcggcctgga caatccggcc aaactgcagc
    17881 agcaccgcct tctcggtctg caacacgatg tagaagcagt tccacccgat cagcgccagc
    17941 acggcagcag caatcagggc gatcagcgac ttgttgctca tcagcggctc tccctcgtac
    18001 gcaggtcacg ctgcaggtcg accgccgccc gcgacgcagg ctcggccgcc ccggcctggg
    18061 cactcggcgc taccgcaccg gccgccggcg cccggctatc gatcatcttg tccagcggca
    18121 gatagagtag gttgttctgt ccctgctcac cggtgaccag caccttgctg gtgttgctga
    18181 gcatttcctg catggtttcc agatacaggc gctcacgggt cacgtccggt gccttgcgat
    18241 actcggcaac cagccggctg aagcggtcgg cctcgccctg ggcgcgcgcc accacttcct
    18301 cgcgatagcc gttggcatcc tcgacgatgc gctgagcctg gccgcgcgct tccggaatca
    18361 cgccgttggc gtaggcctct gcctgattct tctcgcgctg ctcgtcctcg cgggcacgga
    18421 tcacgtcgtc gaaggcttcc tgcacctcgc gcggcgcggc ggcactctgg atgttcacct
    18481 gggtcaccgt gataccggtc ttgtaggtat cgaggaaacg ctgcaggcgc tccttgacct
    18541 cgcccgccat ctgctcgcga ccctcggtca gcacctgatc catcgaggtc gagccgacca
    18601 cgtgacgcag ggcgctgtcg gtggcgtgct gcaggctgat ttccggctgg tcgacgttga
    18661 gcacgaactc ctgcaggttg ctcacgcggt actgcacggt cagcggcacc tcgatgatgt
    18721 tctcgtcctc ggtgagcatc tgcccctgct tggtgtaggc acgctcgcgg gtgacgttat
    18781 cctggaactt gcggtcgatc ggcgggaaat acaggttcag gcccggcccg acagtttcgt
    18841 agtacttgcc gaagcgcaga atcactgcct gctcctgctc gtcgaccaca tagacggcgc
    18901 tgtacagcca gagcgcggcc agcaggccga agccgatcgc cagcaggccg acaccaccac
    18961 ccttgccggt cccaccatcc gcaccgccac gcttgcgccc cccgaacaga ctgttcaggt
    19021 tgtcctgcag cttgcgcaag gcttcatcca ggtctggcgg cccctgacgg ccgccgccac
    19081 ggcgaccacc ccagggatcc tggtcgttcg agttgccacc acccggctca ttccaagcca
    19141 tagcgttctc cccactgata acgcatagac gcgcatttgg cgcacatccg cccatgctac
    19201 agaaagcccc tgatcagcgg caaaaccgtc gacccgggct ttattgcaaa gtgtgttgtg
    19261 caagaaagtc ttcggacttc cagccctcgc gactgaccag cttgttcagc tccacccgct
    19321 gcaggcgcac cttcaacagg atggcacctt cctcgtcatg ctgctcgtcc tgaaccgcgc
    19381 cgagcgcaaa cagctgcgcc cgcagacgac cgagctgctg cggcaggcgc agggtgccga
    19441 cgaacagctc gctgcccagc agctcgctga tggcctgccg cagcaggtcc agcccctgcc
    19501 cttcgcgggc cgacagccag acgcgcaccg gccggccggt atcgtcgcgc tggatctgcg
    19561 gcgccacatc gggcagcagg tccagcttgt tgtacacctc gagggtcggc agctggtccg
    19621 caccgatctc cttgagcacc gaatacacct gctcgatctg cagctggcgt tccggttcgt
    19681 gggcatcgat aacgtgcagc agcaggtcgg cattgctcga ttcctcgagc gtggcgcgga
    19741 acgcctcgac cagcttgtgc ggcaggtgcc ggatgaagcc cacggtatcg gcgaggatca
    19801 ccccgccgac gtcgtcgaac tcgagacggc gcagggtcgg atcgagcgtg gcaaacagct
    19861 gattggcggc atagacatcc gcggaggtca acgcattgaa cagcgtcgac ttgccggcgt
    19921 tggtatagcc gaccagcgag accaccggca cttccgcgcg cttgcgcccg cgccgtgcca
    19981 gctcacgctg gctgcgcacc ttctccaggc gttgcttgat ctggccaatg cgcacgcgca
    20041 gcaggcgacg gtcagtttca agctgggttt cgccggggcc acgcagaccg ataccaccct
    20101 tctgccgctc caggtgggtc cagccacgca ccagacgcgt gctcaggtga tccagctggg
    20161 ccagctcgac ctgcagcttg ccctcgtggg tccgcgcccg ctgggcgaag atgtcgagaa
    20221 tcagaccggt acggtccagc acgcgacact cgaaaacccg ctcgaggttg cgctcctgac
    20281 taggcgtaag ggtgtggttg aagatgacca ggtcagcctc atcggccttg acctgctcgc
    20341 agatctcctc gaccttgccg ctgccgatca ggaagcgcgc cgtgggctga tgccgcggga
    20401 tggtgacgaa gccgaccacc tcggcgcctg ccgaacgggc cagctcgagg aactcgtcgg
    20461 gatcttcacg cgcctcgggc gcctggcctt ccagatgaac cagaatggcc cgctcaccgc
    20521 cctcatggcg ctcgaagaac aagactggcg actccgctta ttcgtcgctg cccgattcgc
    20581 cctgctcgct gctgggcaga cgtaccggac ggctcggaac cacggtggag atggcgtgct
    20641 tgtagaccat ctggctgaca gtgttcttca gcaggatgac gaactggtcg aacgattcga
    20701 tctgaccctg cagcttgatg ccgttgacca gatagatgga aaccggaacg cgctctttgc
    20761 gcagggtgtt caggtaaggg tcttgtagcg aatgcccttt cgacattgtc cacactcctt
    20821 cagggggtaa gtaattttat gggtaagtcg aaatcgaccc aaagtggctt attcctgcac
    20881 ttttttacag gatagccggc aacgggcgaa actcagtctt taatggcgat cgtttgcagg
    20941 tatttcaagg tgcgtgccag attgtcgcag gccaggctgt cgagccagtg caactgcgac
    21001 caactgcgca gccaggtgaa ttgtcgtttg gccagctggc gggtggcgat gatgccgcgt
    21061 tcggtcatct cgtcccgggt cagacgtcct tcgatatatt cccatacctg ccggtagccc
    21121 accgagcgga tcgagggcag tcccggatgc aggtcgccac gggcatgcag cgcttcgacc
    21181 tccgcgatga actcctgttc cagcatgaga ttgaaacgcc gggcaatgcg ctcatgcaat
    21241 acctgacgct gagctggagc gatgaccaat tgcgcgacag tataaggcaa agcctgtccc
    21301 tgcggcgtac cggcgtgcag attttgcaac acctggcgct gccggtgttc gctcatgctc
    21361 aacccgctga cgcggtagac ctccagcgcc cggatcagac gctgcggatc gttgggatgg
    21421 atgcgtgccg ccgactccgg atcgacccg
//
LOCUS       DATCUN010000136         9655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_212384, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000136 DATCUN010000000
VERSION     DATCUN010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9655
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212384"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="VIO83_05550"
     CDS             <1..1287
                     /locus_tag="VIO83_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY8330772.1"
                     /translation="AALAERIAAALLAGQRPLIVAGASLGSKALIEAAGNIAKALKQR
                     EKNAGISLVASEANSLGLTLLGGESVDAALDALSAGQADALVVLENDLYRRADAAKVD
                     AALKAAKVLIVADHQHTATSARAHLVLPAASFAEGDGTLVSQEGRAQRFFQVFDASYY
                     NADNLVREGWRWLHALHSTLQGRPVDWTQLDQVTAACAAVQPELAGIVDAAPTAAFRI
                     KGLKLAREPLRYSGRTSMRANISVHEPRQPQDQDSAFAFSMEGYSGSAEDRQQIPFAW
                     SPGWNSPQAWNKFQDEVGGHLRAGDPGVRLFDAPADRLGWFAVPAAQVGAAGRWQVVA
                     LHHIFGSDETSSRAAPVQERIQAAYAALGSEDAARLGLNDGALLGFTLAGQTLRLPLR
                     VDEELASGLLGLPSGFDGIPPLVGKLFAETLQEVAQ"
     gene            1284..2273
                     /gene="nuoH"
                     /locus_tag="VIO83_05555"
     CDS             1284..2273
                     /gene="nuoH"
                     /locus_tag="VIO83_05555"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY8330773.1"
                     /translation="MSWFTPEVIAAIVAVLKAIVILLAVVVCGALLSFVERRLLGLWQ
                     DRHGPNRVGPYGMFQIAADMLKMFFKEDWTPPFADKLIFTLAPIVAMSALLIAFSVIP
                     VTETWGVADLNIGLLFFFAMAGLTVYAVLFAGWSSNNKFALLGALRASAQTLSYEVFL
                     GLSLMGIVAQVGSFNMRDIVDYQAQNLWFIIPQFLGFCTFFIAGVAVTHRHPFDQPEA
                     EQELADGYHIEYAGMKWGMFFVGEYIGIVLVSALLVTLFFGGWHGPFGILPQLSFVWF
                     ALKTCFFIMLFILLRASIPRPRYDQMMAFGWKFCLPLTLLNLLVTGTLVLAAQ"
     gene            2285..2833
                     /gene="nuoI"
                     /locus_tag="VIO83_05560"
     CDS             2285..2833
                     /gene="nuoI"
                     /locus_tag="VIO83_05560"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014718911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY8330774.1"
                     /translation="MFKYIGEVLHGTWTQLRSLVMVFSHTFRKRDTLQYPEQPVYLPP
                     RYRGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAEKDDGRWYPEFFRINFSRCI
                     FCGMCEEACPTTAIQLTPDFEMAEYKRQDLVYEKEDLLISGPGKNPDYNFYRVAGMAV
                     AGKPKGAAQNEAEPINVKGLLP"
     gene            2848..3357
                     /gene="nuoJ"
                     /locus_tag="VIO83_05565"
     CDS             2848..3357
                     /gene="nuoJ"
                     /locus_tag="VIO83_05565"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY8330775.1"
                     /translation="MEFAFYFAAGVAVASTLRVITHTNPVHALLYLVVSLLAVSMTFF
                     SLGAPFAGVLEIIVYAGAIMVLFVFVVMMLNLGPATADQERQWLTPGVWIGPGLLSAA
                     MLALLLYVLLGNSSGAAIGLSTIDAKAVGTHLYGPYLLAVELASMLLLAALVAAYHLG
                     RHEAKEPTA"
     gene            3354..3662
                     /gene="nuoK"
                     /locus_tag="VIO83_05570"
     CDS             3354..3662
                     /gene="nuoK"
                     /locus_tag="VIO83_05570"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY8330776.1"
                     /translation="MNAIPMEHGLALAAVLFSLGLVGLMVRRNILFVLMSLEIMMNAC
                     ALAFVVAGSRWIQADGQILFILVITLAAAEASIGLAILLQLYRRFNTLDIDAASEMRG
                     "
     gene            3659..5509
                     /gene="nuoL"
                     /locus_tag="VIO83_05575"
     CDS             3659..5509
                     /gene="nuoL"
                     /locus_tag="VIO83_05575"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY8330777.1"
                     /translation="MNLLFLTCLFPLLGWLLLAFSRGRLSENLAALIGVGSVGLSALT
                     AAWVIWQFNVAPPEGGVYTQLLWQWMNVAGFAPNFTLYLDGLSVTMLGVVTGVGFLIH
                     LFASWYMRGEEGYSRFFAYTNLFIASMLFLVLGDNLLFLYFGWEGVGLCSYLLIGFYY
                     SNPNNGNAALKAFIVTRIGDVFMAIGLFILLLNLGTLNIQELLVLAPQKYVAGDTWLW
                     LATLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTNGLFL
                     LTPEILELVGIVGGVTLVLAGFAALVQTDIKRILAYSTMSQIGYMFLALGVGAWEAAI
                     FHLMTHAFFKALLFLASGSVIHACHHEQNIFKMGGLWKKLPLAYASFIVGGAALAALP
                     LITAGFYSKDEILWEAFASGHSGLLYAGLAGAFMTSLYTFRLIFIAFHGEAKTEAHAG
                     HGIAHNLPLAVLIVLSTVVGALITPPLAGVLPQSVGHAGGEAQHSLELVSGAIAILGI
                     VLAALLFLGKRSLVTAVAQSAPGRFLSAWWFAAWGFDWLYDKLFVQPYLLLCRLLARD
                     PIDASIGLLPLLARGSHVALRRSETGNLRWYATSIVGGAVFVLAALLLLN"
     gene            5525..7051
                     /gene="nuoM"
                     /locus_tag="VIO83_05580"
     CDS             5525..7051
                     /gene="nuoM"
                     /locus_tag="VIO83_05580"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY8330778.1"
                     /translation="MILPWLILIPFIGGLLCWQAERFGAALPRWIALLSMSLLLVLGL
                     WLWGSADFTLAPAPGGEPAWASEFQIPWIERFGISIHLALDGLSVLMIVLTGLLGVLS
                     VLCSWNEITKRVGFFHLNLLWILGAVVGVFLAIDLFLFFFFWEMMLVPMYFLIALWGH
                     SGSSGKSRITAATKFFIYTQASGLIMLVAILALAFIHYSQTGVLTFSYADLLQTELAS
                     GTEYLLMLGFFIAFAVKFPVVPLHSWLPDAHAQAPTAGSVDLAGILLKTAAYGLIRFC
                     LPLFPDASAQFAPIAMGLGVFAIVYGALAAFAQTDIKRLVAYSSVSHMGFILIAIYSG
                     SLMALQGAVVQMIAHGLSAAALFILSGQLYERLHTRDLRAMGGIWGRMPWLPAISLFF
                     AAAALGLPGTGNFVGEILVLLGTFQVQPWIAVLAATGLVFGSVYSLCMIHRAYFGQAK
                     ATTALPGLKARELVMVLGLAVLLILLGVYPQPVFDTSAASMQGVQQWLGAALSQLEAR
                     "
     gene            7060..8517
                     /gene="nuoN"
                     /locus_tag="VIO83_05585"
     CDS             7060..8517
                     /gene="nuoN"
                     /locus_tag="VIO83_05585"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEY8330779.1"
                     /translation="MEFTTQHLTALLPLLVTGATAVVVMLAIAWKRNHSQTFLLSVVG
                     LNLALLALIPALGVAPLEVTPLLRIDSFAAFYSAVILAATLACLTLTQAYLGGASGKG
                     YPGNREELYLLMLLACAGGIVLVSAQHLAGLFIGLELLSVPTYGLIAYAFFNRRSLEA
                     GIKYMVLSAAGSAFLLFGMALLYAESGSLSFAAIGATLAAGAPAQLVQIGVGMMLIGL
                     AFKLSLAPFHLWTPDVYEGAPAPVSAFLATASKVAVFAVLLRLYQLSPAFADGWLGNL
                     LSLLAVASILVGNLLALLQSNLKRLLGYSSIAHFGYLLIALIASKGIATEAIGVYLFT
                     YVLTSLGAFGVITLMSTPFQGRDCDALFEYRGLFWRRPYLTAVLATMMLSLAGIPLTA
                     GFIGKFYVVAAGVESQQWWLLGTLILGSAIAVFYYLRVMVTLFLVEPSLQRRDAPLQW
                     AQRAGGIMLLVVAGLTLLLGVYPQPLLAVVQNVGF"
     gene            complement(8717..9187)
                     /locus_tag="VIO83_05590"
     CDS             complement(8717..9187)
                     /locus_tag="VIO83_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEY8330780.1"
                     /translation="MNTLLKPLAVALALLTTGARAETIGEVSTVFKLLGPNHKIVVEA
                     FDDPKVEGVTCYLSRAKTGGIKGGLGLAEDRAEASIACRQVGPIRFLEELEDGEEVFK
                     ERTSLVFKTMQVVRFFDQKRNTLVYMVYSDRIIEGSPQNSVTAIPILPWTKAAN"
     gene            complement(9196..>9655)
                     /locus_tag="VIO83_05595"
     CDS             complement(9196..>9655)
                     /locus_tag="VIO83_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HEY8330781.1"
                     /translation="KLRAARLAARSGANTIIVGGRIEQVLDRLKAGERLGTLLTPERG
                     RHAARKQWLAGHLQTRGTLELDAGAVRALVEQRKSLLPVGVTAVEGNFRRGEMVVCVG
                     PDGLEVARGLVNYSAADALRIIGLTTEAIEQELGYIDEPELVHRDNLVRV"
BASE COUNT         1336 a         3489 c         3027 g         1803 t
ORIGIN      
        1 gctgccctcg ccgaacgcat cgccgccgcc ctgctggccg gccagcgccc gctgatcgtc
       61 gccggcgcct cgctgggctc caaggccctg atcgaggccg ccggcaacat cgccaaggcg
      121 ctcaagcagc gcgagaagaa cgccggcatc agcctggtcg ccagcgaggc caacagcctc
      181 ggcctgaccc tgctgggcgg cgaatcggtc gacgccgcgc tggacgccct gagcgccggc
      241 caggccgacg ccctggtggt gctggagaac gacctctacc gccgcgccga cgccgccaag
      301 gtggacgccg cgctcaaggc cgccaaggtg ctgatcgtcg ccgatcacca gcacaccgcc
      361 accagcgccc gcgcccatct ggtgctgccg gcggccagct tcgccgaagg cgacggcacc
      421 ctggtcagcc aggaaggccg cgcccagcgc ttcttccagg tgttcgacgc cagctactac
      481 aacgccgaca acctggtccg cgagggctgg cgctggctgc acgccctgca cagcaccctg
      541 cagggccggc ccgtcgattg gacccagctg gatcaggtca ccgccgcctg cgccgccgtg
      601 cagccggaac tggccggcat cgtcgatgcc gcgccgaccg ccgcgttccg catcaagggc
      661 ctcaagctgg cccgtgaacc gctgcgctac agcggccgca cctcgatgcg cgccaacatc
      721 agcgtgcacg agccgcgtca gccgcaggac caggacagcg ccttcgcctt ctccatggaa
      781 ggctactccg gcagcgccga agaccgtcag cagatcccct ttgcctggtc gccgggctgg
      841 aactcgccgc aggcctggaa caagttccag gacgaagtcg gcggccacct gcgcgccggc
      901 gatccgggcg tgcgcctgtt cgacgccccg gccgaccgcc tcggctggtt cgccgtaccg
      961 gcggcccagg tcggcgctgc cggtcgctgg caggtcgtcg ccctgcacca catcttcggc
     1021 agcgacgaga cctcctcgcg cgccgcaccg gtgcaggagc ggatccaagc cgcctacgcc
     1081 gccctcggca gcgaggatgc cgcgcgtctg ggcctcaacg acggcgccct gctcggcttc
     1141 accctcgccg ggcagaccct gcgcctgccg ctgcgcgtcg acgaggaact ggccagcggt
     1201 ctgcttggcc tgccgagcgg cttcgacggc attccgccgc tggtcggcaa gctgttcgcc
     1261 gaaaccctgc aggaggtcgc gcaatgagct ggttcacacc ggaagtgatc gcggcgatcg
     1321 tcgccgtgct caaggccatc gtcatcctgc tggcggtggt ggtctgcggc gccctgctga
     1381 gcttcgtcga gcggcgcctg ctcggcctct ggcaggatcg ccacggcccc aaccgcgtcg
     1441 gtccctacgg catgttccag atcgccgcgg acatgctgaa gatgttcttc aaggaagact
     1501 ggacgccgcc gttcgccgac aagctgatct tcaccctggc gccgatcgtc gccatgagtg
     1561 cgctgctgat cgccttctcg gtgatcccgg tgaccgagac ctggggcgtg gcggatctga
     1621 acatcggcct gctgttcttc ttcgccatgg ccggcctgac tgtgtacgcg gtgctgttcg
     1681 ccggctggtc gagcaacaac aagttcgccc tgctcggcgc cctgcgcgcc tcggcgcaga
     1741 cgctgtccta cgaggtgttc ctcggcctgt cgctgatggg catcgtcgcc caggtcggct
     1801 cgttcaacat gcgcgacatc gtcgactatc aggcacagaa cctgtggttc atcattccgc
     1861 agttcctcgg cttctgcacc ttcttcatcg ccggcgtcgc ggtgacccac cgtcacccgt
     1921 tcgaccagcc ggaggccgag caggaactgg ccgacggcta ccacatcgag tacgcgggga
     1981 tgaagtgggg catgttcttc gtcggcgagt acatcggcat cgtgctggtg tcggcgctgc
     2041 tggtgaccct gttcttcggc ggctggcacg gcccgttcgg catcctaccg caactgtcgt
     2101 tcgtctggtt cgccctcaag acctgcttct tcatcatgct gttcatcctg ctgcgcgcct
     2161 ccattccgcg cccgcgctat gaccagatga tggccttcgg ctggaagttc tgcctgccgc
     2221 tgaccctgct caacctgctg gtgaccggca ccctggtgct ggctgcccag taaggagaaa
     2281 cacaatgttc aaatacattg gcgaggtgct gcacggcacc tggacccagc tgcgcagcct
     2341 ggtgatggtg ttctcccaca ccttccgcaa gcgcgacacc ctgcagtacc ccgagcaacc
     2401 cgtgtacctg ccgccgcgct accgcggccg catcgtgctg acccgcgacc ccgacggcga
     2461 ggagcgctgc gtggcctgca acctgtgcgc ggtggcctgc ccggtgggct gcatctcgct
     2521 gcagaaggcc gagaaggacg acggccgctg gtatccggag ttcttccgca tcaacttctc
     2581 gcgctgcatc ttctgcggca tgtgcgagga agcctgcccg accaccgcga tccagctcac
     2641 cccggacttc gagatggccg agtacaagcg ccaggacctg gtctacgaga aggaagacct
     2701 gctgatctcg ggcccgggca agaacccgga ctacaacttc taccgcgtcg ccggcatggc
     2761 tgttgccggc aagccgaaag gcgccgcgca gaacgaggcc gagccgatca acgtgaaagg
     2821 cctgctgcct taaaggacgg aagaaagatg gaattcgcct tctatttcgc cgccggagtg
     2881 gccgtggcat ccaccctgcg ggtgatcacc cacaccaacc cggtgcatgc cctgctctac
     2941 ctggtggttt cgctgctggc cgtgtcgatg accttcttca gcctcggcgc cccgttcgcc
     3001 ggcgtgctgg agatcatcgt ctacgccggc gccatcatgg tgctattcgt cttcgtggtg
     3061 atgatgctca acctcggccc ggccaccgcc gaccaggaac gccagtggct cacccccggc
     3121 gtgtggatcg gtcccggcct gctctcggcg gccatgctgg ccctgctgct gtacgtgctg
     3181 ctgggcaact ccagcggtgc cgccatcggt ctgtcgacca tcgacgccaa ggcggtcggc
     3241 acccacctgt acggtcccta cctgctggcg gtggaactgg cttccatgct gctgctggca
     3301 gccctggtcg ccgcctacca cctcggccgc cacgaagcca aggagcccac cgcatgaatg
     3361 cgattcccat ggagcacggc ctggccctgg ccgccgtgct gttcagtctc ggcctggtcg
     3421 gcctgatggt gcggcgcaac atcctgttcg tgctgatgag cctggaaatc atgatgaacg
     3481 cctgcgcgct ggccttcgtg gtcgccggca gtcgctggat ccaggctgac ggccagatcc
     3541 tgttcattct ggtgatcacc ctggccgccg ccgaggccag catcggcctg gcgatcctgc
     3601 tgcagctgta tcgccgcttc aataccctcg atatcgacgc tgcaagcgag atgcgcggat
     3661 gaacctacta ttcctcacct gtctgtttcc gctgctcggc tggctgctgc tggccttctc
     3721 ccgcggccgt ctgtccgaga acctggccgc gctgatcggc gtcggctcgg tgggcctgtc
     3781 ggcgctgacc gccgcctggg tcatctggca gttcaatgtc gcgccgccgg aaggtggcgt
     3841 gtacacccag ctgctctggc agtggatgaa cgtcgccggc ttcgcgccga acttcaccct
     3901 gtatctggac ggcctgtcgg tcaccatgct cggcgtggtc accggcgtcg gcttcctgat
     3961 ccacctgttc gccagctggt acatgcgcgg cgaggagggc tactcgcgct tcttcgccta
     4021 caccaacctg ttcatcgcca gcatgctgtt cctggtgctc ggcgacaacc tgctgttcct
     4081 ctacttcggc tgggaaggcg tgggcctgtg ctcgtacctg ctgatcggct tctactacag
     4141 caacccgaac aacggtaacg ccgcgctcaa ggccttcatc gtcacccgta tcggcgacgt
     4201 gttcatggct atcggcctgt tcatcctgct gctcaacctg ggcacgctga acatccagga
     4261 actgctggtg ctggcgccgc agaagtacgt ggcgggcgac acctggctgt ggctggccac
     4321 cctgatgctg ctcggcggtg cggtcggcaa gtccgcccag ctgccgctgc agacctggct
     4381 ggccgacgcc atggccggcc cgaccccggt ctcggcgctg atccacgcgg cgaccatggt
     4441 caccgccggc gtctacctga tcgcgcgcac caacggcctg ttcctgctga ccccggagat
     4501 cctcgagctg gtcggcatcg tcggcggcgt gaccctggtg ctggccggct tcgccgcgct
     4561 ggtgcagacc gacatcaagc gcatcctcgc ctactcgacc atgagccaga tcggctacat
     4621 gttcctcgcc ctcggcgtcg gtgcctggga ggcggcgatc ttccacctga tgacccacgc
     4681 cttcttcaag gccctgctgt tcctcgcctc cggctcggtg atccacgcct gccaccacga
     4741 gcagaacatc ttcaagatgg gcggcctgtg gaagaagctg ccgctggcct atgccagctt
     4801 catcgtcggc ggcgcggcgc tggccgccct gccgctgatc accgccggct tctactccaa
     4861 ggacgagatc ctctgggaag ccttcgccag cggtcacagc ggcctgctct acgccggtct
     4921 ggccggtgcg ttcatgacct cgctgtacac cttccggctg atcttcatcg ccttccacgg
     4981 cgaggcgaag accgaggcgc atgccggcca cggcattgcc cacaacctac cgctggcggt
     5041 gctgatcgtg ctgtccaccg tggtaggcgc gctgatcacc ccgccactcg ccggcgtgct
     5101 gccgcagagc gtcggccatg ccggcggcga agcccagcac agcctggagc tggtttccgg
     5161 cgccatcgcc atcctcggca tcgtcctcgc cgccctgctg ttcctcggca agcgcagcct
     5221 ggtgaccgcc gtggcgcaga gcgcgccggg tcgcttcctg tcggcctggt ggttcgcggc
     5281 ctggggcttc gactggctgt acgacaagct gttcgtgcag ccctacctgc tgctctgccg
     5341 cctgctcgct cgcgatccca tcgacgccag catcggtctg ctgccactgc tggcccgtgg
     5401 cagccacgtc gccctgcgcc gcagcgaaac cggcaacctg cgctggtacg ccacctccat
     5461 cgtcggcggg gccgtgttcg tgctcgctgc ccttctgctt ctgaattaag gaaaacggcc
     5521 cgtcatgatt ctgccctggc taatcctgat tcccttcatc ggcggcctgc tgtgctggca
     5581 ggccgagcgc ttcggcgccg ccctgccgcg ctggatcgcg ctgctgagca tgtccctgct
     5641 gctggtcctc ggcctctggc tgtggggctc cgccgacttc accctggcgc ctgcgccggg
     5701 cggcgagccg gcctgggcca gcgagttcca gatcccgtgg atcgagcgct tcggcatctc
     5761 catccaccta gcgctggacg gcctgtcggt gctgatgatc gtgctcaccg gcctgctcgg
     5821 cgtgctctcg gtactctgct cgtggaacga gatcaccaag cgcgtcggct tcttccacct
     5881 caacctgctg tggatcctcg gcgcggtggt cggcgtgttc ctcgccatcg acctgttcct
     5941 gttcttcttc ttctgggaaa tgatgctggt gccgatgtac ttcctcatcg cgctctgggg
     6001 tcacagcggc agcagcggca agagccggat caccgccgcc accaagttct tcatctacac
     6061 ccaggccagc ggcctgatca tgctggtggc gatcctcgcc ctggcgttca tccactacag
     6121 ccagaccggc gtgctgacct tcagctacgc cgacctgctg cagaccgaac tggcgtcggg
     6181 taccgagtac ctgctgatgc tcggtttctt catcgccttc gcggtgaagt tcccggtggt
     6241 gccgctgcac tcctggctgc cggacgccca cgcccaggcg ccgaccgccg gctccgtgga
     6301 cctggccggc atcctgctga agaccgcggc ctacggcctg atccgcttct gcctgccgct
     6361 gttccccgac gcctcggcgc agttcgcgcc gatcgccatg ggcctcggcg tattcgccat
     6421 cgtctacggc gccctggcgg ccttcgcgca gaccgacatc aagcgcctgg tggcctactc
     6481 cagcgtgtcg cacatgggct tcatcctgat cgccatctac tccggtagtc tgatggcgct
     6541 gcagggtgcg gtggtgcaga tgatcgccca cggcctgtcc gccgcggcgc tgttcatcct
     6601 ctccggccag ctctacgagc gcctgcacac ccgcgacctg cgcgcgatgg gcggcatctg
     6661 gggacgcatg ccgtggctgc cggcgatcag cctgttcttc gccgccgcgg cgctgggcct
     6721 gcccggcacc ggcaacttcg tcggcgagat cctcgtgctg ctcggtacct tccaggtgca
     6781 gccgtggatc gccgtgctgg ccgccaccgg cctggtgttc ggctcggtct attcgctgtg
     6841 catgatccat cgcgcctact tcggccaggc caaggcaacc actgccctgc ccggtctcaa
     6901 ggcccgcgaa ctggtcatgg tcctcggcct ggccgtgctg ctgatcctgc tgggtgtcta
     6961 cccgcagccg gtgttcgaca cctcggccgc cagcatgcag ggcgtacagc aatggctggg
     7021 cgccgccctc tcacaacttg aggccaggta attagcgcta tggaattcac tactcaacac
     7081 ctgaccgccc tgctgccgct gctggtcacc ggcgccaccg ccgtcgtcgt gatgctggcg
     7141 atcgcctgga aacgcaacca cagtcagacc ttcctgctct cggtggtcgg cctcaacctg
     7201 gccctgctgg cgctgatccc cgccctcggc gtggcgccgc tggaggtcac cccgctgctg
     7261 cgcatcgaca gcttcgccgc cttctacagc gcggtgatcc tcgccgcgac cctggcctgc
     7321 ctgaccctga cccaagccta cctcggcggg gcgtcgggca agggctatcc gggcaaccgc
     7381 gaggagctgt acctgctgat gctgctggcc tgcgccggcg gcatcgtgct ggtcagcgcc
     7441 cagcacctgg ccggcctgtt catcggcctg gagctgctgt cggtaccgac ctacggcctg
     7501 attgcctacg ccttcttcaa ccgtcgctcg ctggaagccg gcatcaagta catggtgctg
     7561 tcggccgccg gctcggcttt cctgctgttc ggcatggccc tgctgtacgc cgaatccggc
     7621 agcctcagct tcgccgccat cggcgccacg ctggccgccg gcgcgccggc ccagctggtg
     7681 cagatcggcg tgggcatgat gctgattggc ctggccttca agctgtcgct ggcgcccttc
     7741 cacctgtgga ccccggacgt ctacgagggc gccccggcgc cggtgtcggc gttcctcgcc
     7801 accgccagca aggtcgccgt gttcgccgtg ctgctgcgcc tgtaccagct ctcgccggcc
     7861 ttcgccgacg gctggctggg caacctgctg agcctgctgg cggtcgcctc gatcctggtc
     7921 ggcaacctgc tggcgctgct gcagagcaac ctcaagcgcc tgctcggtta ctcgtcgatc
     7981 gcccacttcg gctacctgct gatcgccctg atcgccagca agggcatcgc caccgaggcc
     8041 atcggcgtct acctgttcac ctacgtgctg accagcctgg gcgccttcgg cgtgatcacc
     8101 ctgatgtcga cgccgttcca gggccgcgac tgcgacgcgc tgttcgagta ccgcggcctg
     8161 ttctggcgcc gcccgtacct gaccgcggtg ctggccacca tgatgctgtc gctggcgggc
     8221 atcccgctga ccgccggctt catcggcaag ttctacgtgg tcgccgctgg tgtcgaaagc
     8281 cagcagtggt ggctgctcgg caccctgatc ctcggcagcg ccatcgcggt gttctactac
     8341 ctgcgggtga tggtcaccct gttcctggtc gagcccagcc tgcagcgccg cgacgcgccg
     8401 ctgcagtggg cccagcgcgc cggcggcatc atgctgctgg tggtcgccgg cctgaccctg
     8461 ctgctcggcg tctacccgca gccgctgctc gccgtggtgc agaacgtcgg cttctgaggt
     8521 aaatctcagg catgaaaaag ccggtcgggg caacccgacc ggcttttttt gtggcaacta
     8581 ctgtccgcgc agggtgggtt aggccgcagg ccgtaaccca ccgagccggc cgtcaggccg
     8641 gccattagcg atgcctggcg gcagcgatgg tgggttacgc cgctgcgcgg ctaacccacc
     8701 ctacggagca gcggaatcag ttcgctgctt tcgtccacgg caggatcggg atggcggtga
     8761 ccgagttctg cgggctgcct tcgatgatgc ggtcgctgta gaccatgtac accagcgtgt
     8821 tgcgcttctg gtcgaagaag cgcaccacct gcatggtctt gaacaccagc gaggtgcgct
     8881 ccttgaacac ctcctcgccg tcttccagct cttcgaggaa gcggatcggg ccgacctggc
     8941 ggcaggcgat cgacgcctcg gcgcggtcct cggcgagacc caggccgccc ttgatgccgc
     9001 cggtcttggc ccgcgacagg tagcaagtca ccccctccac cttgggatcg tcgaacgcct
     9061 cgacgacgat cttgtggttg gggccgagca gcttgaacac cgtggagact tcgccgatgg
     9121 tttcggcccg ggcgcccgtg gtgagcagcg ccagggcaac ggccagcggc ttgagcagcg
     9181 tgttcatggc aatcctcata cccgtaccag gttgtcgcga tgcaccagtt ccggctcgtc
     9241 gatgtagccg agttcctgct cgatggcttc ggtggtcagg ccgatgatgc gcagggcatc
     9301 ggcggcactg tagttgacca gcccgcgcgc cacctccagg ccgtccggac cgacgcagac
     9361 caccatctcg ccgcggcgga agttgccttc cacggcggtg acgccgaccg gcagcaggct
     9421 cttgcgctgt tcgaccagcg cgcgcaccgc accggcgtcc agctccaggg tgccgcgggt
     9481 ctgcaggtgg ccggccagcc actgcttgcg cgcggcatgc cgaccccgct cgggagtcag
     9541 cagggtgccg aggcgctcgc ccgccttgag gcggtccagc acctgctcga tgcgcccgcc
     9601 gacgatgatg gtgttggccc ccgaacgcgc ggccaggcgc gccgcgcgca gcttg
//
LOCUS       DATCUN010000137         2643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_61872, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000137 DATCUN010000000
VERSION     DATCUN010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61872"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VIO83_05600"
     CDS             <1..483
                     /locus_tag="VIO83_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEY8330782.1"
                     /translation="PRTAYIMTSMLQDVIKRGTGRRALSLQRSDLAGKTGTTNEQKDS
                     WFAGYNGDFVTTAWVGFDQPQSLGHNEYGGTVALPIWIRYMGVALKDKPAHLPPEPEG
                     LLRLRVDPLTGQAAAAGSSGAFFELFNKDAPPQPAREASNSFQDNFGSEPAAVPIDLF
                     "
     gene            complement(542..1810)
                     /locus_tag="VIO83_05605"
     CDS             complement(542..1810)
                     /locus_tag="VIO83_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEY8330783.1"
                     /translation="MSDLKAAALEYHAHPRPGKLSVELTKPTATARDLALAYSPGVAE
                     PVREIARDPELAFKYTGKGNLVAVISDGTAILGLGNLGPLASKPVMEGKGVLFKRFAG
                     VDVFDIEVDAESPQAFIDTVKRISITFGGINLEDIKAPECFEIERALIEQCDIPVFHD
                     DQHGTAIVTAAGMLNALEIVGKKLEEAKIVCLGAGAAATACMKLLVSIGANIENIFMI
                     DRKGVIHAGRDDLNQYKSVFAHETDKRTLSDAMEGADVFVGLSGANLLPAEDLARMAP
                     TPIVFACSNPDPEIAPALAHATRPDVIMATGRSDYPNQVNNVLGFPFIFRGALDVRAT
                     RINEEMKIAAVNAIRELAKLPVPKEVCEAYGVDNLEFGREYIIPKPMDQRLIQLVPDA
                     VAKAAIETGVARLGYPRHYPLTSVDDVFNG"
     gene            complement(2033..>2643)
                     /locus_tag="VIO83_05610"
     CDS             complement(2033..>2643)
                     /locus_tag="VIO83_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY8330784.1"
                     /translation="NAPEKARGERPAEPLAEAATRHLQQLVDASGGRVGLLAGTQARD
                     RYGRSLAHAFGRDGRNWEAQLLAAGLGFAVAMAPNTALVDCQFAAEGAARAAGRGVWR
                     LTPLLETSRLRRGGFALLRGRVATVERNRGGLWLELDGSLVLQVEAQSLALFDERALR
                     ALAGRTVVVRGWVVDRSRRGALKPGQARWLLRLSHPAMLEPR"
BASE COUNT          474 a          911 c          842 g          416 t
ORIGIN      
        1 ccgcgcaccg cctacatcat gaccagcatg ttgcaggacg tgatcaagcg cggcaccggc
       61 cggcgcgccc tgagcctgca gcgcagcgac ctggccggca agacgggcac caccaacgaa
      121 cagaaggact cctggttcgc cggctacaac ggcgacttcg tcaccaccgc ctgggtcggc
      181 ttcgaccagc cgcaatcgct cggccacaac gaatacggcg gcaccgtcgc cctgccgatc
      241 tggatccgct acatgggggt agccctcaag gacaagccgg cacacctgcc gcccgagccg
      301 gaaggcctgt tgcgcctgcg cgtcgatccg ctcaccggcc aagccgctgc ggccggcagc
      361 agcggagcct tcttcgagct gttcaacaag gacgccccgc cacagcccgc ccgggaagcc
      421 agcaacagct tccaggacaa tttcggcagc gaacccgccg ccgtccccat cgacctgttc
      481 tgatttcagg caggcacaaa aaaacccgct tctgcgggtt tttttgtcac tcgctggccg
      541 atcagccgtt gaacacgtca tccaccgagg tcagcggata gtggcgcggg taaccgaggc
      601 gggccacgcc ggtctcgatg gccgccttgg ccaccgcgtc cggcaccagc tggatcaggc
      661 gctggtccat cggcttcgga atgatgtact cgcgaccgaa ctccaggttg tccacgccat
      721 aggcttcgca gacttccttg ggcaccggca gcttggccag ttcgcggatg gcgttgaccg
      781 cggcgatctt catttcctcg ttgatccggg tggcgcgcac atccagcgca ccgcggaaga
      841 tgaacgggaa gccaaggacg ttgttgacct ggttcgggta gtcggagcgg ccggtagcca
      901 tgatcacgtc cggacgggtg gcatgcgcca gcgccggggc gatttccgga tccggattgg
      961 agcaggcaaa cacgatcggg gtcggcgcca tgcgcgccag atcttcggct ggcagcaggt
     1021 tggcaccgga caggccgaca aacacgtcag caccttccat ggcatcggac agggtgcgct
     1081 tgtcggtttc gtgggcgaat accgacttgt actggttcag atcgtcgcgg ccggcgtgga
     1141 tcacgccctt gcggtcgatc atgaaaatgt tctcgatatt cgcaccgatg ctgaccagca
     1201 gcttcatgca tgcggtcgcc gcggcaccag cgccgaggca gacgatcttc gcctcttcca
     1261 gcttcttgcc gacgatttcc agggcgttga gcatgccggc cgcggtgacg atggcagtgc
     1321 cgtgctggtc gtcgtggaac accgggatgt cgcactgctc gatcagcgcg cgctcgatct
     1381 cgaagcactc gggcgccttg atgtcttcca gattgatgcc gccgaaggtg atggagatgc
     1441 gcttgacggt atcgatgaag gcctgcgggc tttccgcatc cacttcgata tcgaacacgt
     1501 cgacgccggc gaagcgcttg aacagcacgc ccttgccttc catcaccggc ttggacgcca
     1561 gcgggccgag gttgcccagg ccgaggatcg cggtgccatc ggagatcacc gccaccaggt
     1621 tgcccttgcc ggtgtacttg aaggccagct cgggatcgcg agcgatctcg cgcaccggct
     1681 cggcaacgcc gggactgtag gccagggcca ggtcgcgcgc ggtggcagtg ggcttggtca
     1741 gctcgacact cagcttgccg ggacggggat gagcgtgata ctcgagcgcg gcggctttca
     1801 gatcagacat attggtgctt ccactttcta ttttatttag gtacagacag ggaccctgag
     1861 catacggcaa gagcgcgaac ccccacaaga gcgtgactcc agagcctcga caggctgtct
     1921 ccaggcctat ccacgggtac gcttggccag cacgccagcc accaaaacag agcgacgaaa
     1981 ggggtcgcca cggaccagcg gaaccgggta cagacgcaca gcgccccggc cttcagcgcg
     2041 gctcaagcat ggccggatga ctcagccgca gcagccagcg cgcctgccca ggcttgagtg
     2101 cgccacgccg gctgcgatcg actacccagc cgcgcaccac gaccgtgcgc cccgccagtg
     2161 cgcgcagcgc acgctcgtcg aacagcgcca gcgactgcgc ctcgacctgc agcaccagcg
     2221 agccgtccag ctccagccag agaccgccgc gattgcgctc gaccgtcgcg acccgcccgc
     2281 gcagcaaggc aaagccgcca cggcgcaagc gggaagtctc gagcagcggc gtcaggcgcc
     2341 agacaccgcg gcccgccgcc cgtgcagcac cttcggcagc gaactggcag tcgaccagcg
     2401 cggtgttcgg tgccatggcc acggcaaagc ccagtcccgc ggccaacagc tgcgcttccc
     2461 agttgcggcc gtcgcgaccg aaggcatgcg ccaggctgcg cccgtagcgg tcgcgcgcct
     2521 gcgtgccggc cagcagacca acgcgcccgc cgctggcatc gaccaactgc tgcagatggc
     2581 gggtggcggc ctcggccagc ggctcggccg gacgctcgcc gcgggccttt tccggcgcat
     2641 tca
//
LOCUS       DATCUN010000138         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_155992, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000138 DATCUN010000000
VERSION     DATCUN010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155992"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VIO83_05615"
     CDS             complement(<1..792)
                     /locus_tag="VIO83_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8330785.1"
                     /translation="MRDDALASLRLMPSVERPPQVFVRGQGSWLWDSEDRAYLDFTQG
                     GAVNSLGHSPSVVVEALKRQAERLINPGAGYYNAGLLKLASRLCEATGSDQAYFLNSG
                     AEACEGAIKLARKWGQRHRGGAFTIVTAKHSFHGRTLGAMSASGKPAFQQMFEPKLPG
                     FEKVPFNDLEALSQAVDSHTVAVMLEPLQGEAGVIPATLDYLQGAERLCRARGVLLIL
                     DEVQTGMGRCGALLAEELYGVRADIVTLGKGLGGGVPLAAVLARGN"
     gene            complement(785..1501)
                     /locus_tag="VIO83_05620"
     CDS             complement(785..1501)
                     /locus_tag="VIO83_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY8330786.1"
                     /translation="MHAITIREVFIGKFEEIGRAQSSVAAAQLLQGEVWLGSDGLPGD
                     QLAQRRPELVDSALLHYPAEHYRHWRKRFPLCKWRPAACGENFSTFGLGEAEVCVGDV
                     VRWGEALLEVSQPCLPDYGLARRWNLPELPRLLQDSGRCGWFYRVLRPGLVSATEPLQ
                     WVQRHYPELSVARLLEWYVHTPLERCALRQMLACDALAPAWRKVAERRLASGELEDWQ
                     ARLYGGEPARTAESEGVQHA"
     gene            1602..2480
                     /locus_tag="VIO83_05625"
     CDS             1602..2480
                     /locus_tag="VIO83_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8330787.1"
                     /translation="MDFRQLRYFVAVYEEGHVGRAAERLSISQPALSQQIRHLEGELE
                     VALFQRNGKRLLPAPAAHTLYHHAVPLLDGLERTREAMRAFRGQAPRSLAIGVLQTVN
                     ANLVPLLLERLRALQPHLVVQIYELSGIDIERRLLTGTLDVGIGFLPPRQPGLHSLLL
                     YEDELQLVIAADHPLREFRKVSLAQAAELPMLLLGEEFRIRQIWQEQLAAIGRRPQVQ
                     AELNQMNGILDSLAHTQLASVLPGRARQVQGHEQLLWKPLSEPRVPLQVGLVYRDAQR
                     QQATLELLRSVLEGGA"
BASE COUNT          408 a          938 c          869 g          422 t
ORIGIN      
        1 gttgccgcgg gccagcacgg cggccagcgg cacgccgccg ccgaggccct tgccgagggt
       61 gacgatgtcg gcgcgcacgc cgtacagctc ctcggccagt agcgcgccgc aacggcccat
      121 gccggtctgc acctcatcga ggatcagcag cacgccgcgc gcccggcaca gccgctcggc
      181 gccctgcagg tagtcgaggg tcgccgggat caccccggcc tcgccctgca gcggctccag
      241 catcaccgcc acggtatgcg agtcgactgc ctgcgacagc gcctccagat cgttgaacgg
      301 caccttttcg aagcctggca gcttcggttc gaacatctgc tggaaggccg gcttgcccga
      361 ggcggacatg gcgcccaggg tgcggccatg gaaactgtgc ttggcggtga cgatggtgaa
      421 ggcaccgccg cgatggcgct ggccccactt gcgcgccagc ttgatcgcac cctcgcaggc
      481 ctcggcgccg ctgttgagaa agtacgcctg gtcgctgccg gtggcctcgc acaggcgact
      541 ggccagcttg agcaggcccg cgttgtagta gcccgcaccg gggttgatca gccgctcggc
      601 ctggcgcttg agcgcctcga ccaccacgct cgggctgtga ccaaggctgt tgaccgcccc
      661 gccctgggtg aagtcgagat aggcgcgatc ctcgctgtcc cacagccagg agccctggcc
      721 acgcacgaag acctgcggcg ggcgctcgac gctgggcatc aggcgcaggc tcgccagcgc
      781 atcgtcacgc atgctggact ccttcgctct cggcagtccg tgccggttcg ccgccgtaca
      841 ggcgcgcctg ccagtcctct agttcgccgc tggccaggcg ccgttcggcc accttgcgcc
      901 acgccggcgc cagcgcgtcg caggccagca tctggcgcag cgcgcaacgc tccagcgggg
      961 tatgcacgta ccactcgagc agacgggcga cgctcagctc cgggtaatgc cgctgcaccc
     1021 actgcagcgg ctcagtcgca ctgaccaggc cggggcgcag cacccggtag aaccagccgc
     1081 agcgaccgct gtcctgcagc agccgcggca actcgggcag gttccagcgc cgcgccagtc
     1141 cgtagtcggg caggcacggc tggctgactt ccagcagcgc ctcgccccag cgcaccacgt
     1201 cgccgacgca gacctccgcc tcgccgaggc cgaaggtgga aaagttttcg ccacaggcgg
     1261 ccggccgcca cttgcacagc gggaagcgct tgcgccagtg gcgatagtgc tcggccggat
     1321 agtgcagcag ggcgctgtcg accagttccg gccggcgctg ggccaactgg tcgcccggca
     1381 gaccatcgct gcccagccag acctcgccct gcagcagctg cgccgcggcg acgctggatt
     1441 gagcgcggcc gatctcttcg aatttaccta tgaacacttc ccgaatcgtg atggcgtgca
     1501 tgggctcttg accacctgtc tgcgcttgat gagcgctaga ctaggactcg tccggcctgc
     1561 aggccatttc gtttttccag cgtctctaat aagttcagct tatggatttt cgtcagcttc
     1621 gctacttcgt cgccgtctac gaggaaggcc atgtcggccg cgccgccgag cgcctgagca
     1681 tttcccagcc ggcgctgtcg cagcagatcc gccatctgga aggcgaactg gaggtggcgc
     1741 tgttccagcg caacggcaag cgcctgctgc cggcgccggc ggcgcacacc ctctaccacc
     1801 atgccgtgcc gctgctcgac ggcctcgagc gtacccgcga ggcaatgcgc gccttccgcg
     1861 gccaggcacc gcgcagcctg gccatcggcg tgctgcagac ggtcaacgcc aacctggtgc
     1921 cgctgctgct ggagcgcctg cgcgccctgc agccgcatct ggtagtgcag atctacgagc
     1981 tgtcggggat cgacatcgag cggcgcctgc tcaccggcac cctggatgtc ggcatcggct
     2041 tcctgccgcc ccgccagccc ggcctgcaca gcctgctgtt gtacgaggac gagctgcagc
     2101 tggtgatcgc cgccgaccat ccgctgcgcg agttcaggaa ggtgtcgctg gcccaggcgg
     2161 ccgagctgcc gatgctgctg ctcggcgagg agttccgcat ccgccagatc tggcaggagc
     2221 aactggccgc catcggtcgc cggccgcagg tacaggccga gctgaaccag atgaacggca
     2281 ttctcgacag cctggcgcac acccagctgg cctcggtgct gcccggccgg gcgcggcagg
     2341 tgcaggggca cgagcaactg ctgtggaaac cgctgagcga gccgcgcgtg ccgctgcagg
     2401 tggggctggt gtaccgcgac gcccagcgtc agcaggcgac gctggagctg ctgcgctcgg
     2461 tgctggaggg aggcgcctga gcgaaaaatc gcgcacaaaa aaaaccccgc ctaagcgggg
     2521 ctcttgcaga cctgttcctg acatccgtag tcgacccatc gtcctgatgg gatccctgtg
     2581 atccgtgttc tgatttgcgc ttcctgcgcc aagtccagat gtgcttaggt tagttgc
//
LOCUS       DATCUN010000139        23044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_99854, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000139 DATCUN010000000
VERSION     DATCUN010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 23044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23044
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99854"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..936)
                     /gene="cysD"
                     /locus_tag="VIO83_05630"
     CDS             complement(19..936)
                     /gene="cysD"
                     /locus_tag="VIO83_05630"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY8330788.1"
                     /translation="MVDKLTHLKQLEAESIHIIREVAAEFDNPVMLYSIGKDSAVMLH
                     LARKAFFPGKLPFPVMHVDTQWKFQEMYRFRDKMVAELGLDLITYVNPEGVAQGINPF
                     THGSAKHTDIMKTEGLKQALDKYGFDAAFGGARRDEEKSRAKERVYSFRDSKHRWDPK
                     NQRPELWNVYNGKVKKGESIRVFPLSNWTELDIWQYIYLEGIPIVPLYFAAEREVIEK
                     NGTLIMIDDARILEHLSDEEKARIQKKMVRFRTLGCYPLTGAVESTAASLPEIIQEML
                     LTRTSERQGRVIDHDGAGSMEEKKRQGYF"
     gene            complement(1066..1824)
                     /locus_tag="VIO83_05635"
     CDS             complement(1066..1824)
                     /locus_tag="VIO83_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEY8330789.1"
                     /translation="MAVALHTLVAETDRYLDAARIVDYCPNGLQVEGRAEVRRIVSGV
                     TASQALLDAAVEAQADLVLVHHGYFWKNENACITGMKQRRLKTLLSHDISLLAYHLPL
                     DQHPEVGNNVQLARRLGLQVEGPLEPGNPRSVGLLGSLAQALSPAEFAAQVAQVLGRE
                     PLLVPGCGPIRRVAWCTGAAQGYIEQAIAAGVDAYLSGEVSESTVHSARENGISFLAA
                     GHHATERYGVQALGEWLAQRFGLEHRFIDCANPV"
     gene            1931..3073
                     /gene="algW"
                     /locus_tag="VIO83_05640"
     CDS             1931..3073
                     /gene="algW"
                     /locus_tag="VIO83_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase AlgW"
                     /protein_id="HEY8330790.1"
                     /translation="MYKALRYLGWPVVAGILLALLAIQFFPQWVGHGQPDLQLRQAPA
                     LGLPSLGPVSYGDAVELAAPAVANLYTTKVVDKPSHPLFGDPQFRRFFGDNLPRQRRM
                     ESSLGSAVIMSPRGYLLTNNHVTAGADQIIVALKDGRETLARVIGSDPETDLAVLKID
                     LPDLPAITLGRSDNIRIGDVALAIGNPFGVGQTVTMGIISATGRNQLGLNTYEDFIQT
                     DAAINPGNSGGALIDAHGNLIGINTAIFSRSGGSQGIGFAIPTKLAQEVMEAIINHGQ
                     VIRGWLGLEVQPLTTELAESIGLQGQAGIIVAGIYRDSPALHAGLQPGDVILAIDGEP
                     ASDGRRAMNQVARKRPGEKLHIDILRAGEKHALKAEVGLRPPVASE"
     gene            complement(3146..4204)
                     /gene="hisC"
                     /locus_tag="VIO83_05645"
     CDS             complement(3146..4204)
                     /gene="hisC"
                     /locus_tag="VIO83_05645"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY8330791.1"
                     /translation="MSKFWSPFVKDLVPYVPGEQPKMARLIKLNTNENPYGPSPKVLE
                     AIRAELDDSLRLYPDPNGERLKQTIADYYGVQASQVFLGNGSDEVLAHVFHGLFQHGR
                     PLLFPDISYSFYPVYCGLYDIPFETVALDVQFRINVADYERPNGGIIFPNPNAPTGCL
                     LPLAAIRRLLEANTESVVVVDEAYVDFGGESAISLVDQYPNLLVTQTLSKSRSLAGLR
                     VGLAVGNAELIEALERIKNSFNSYPLDRLALVGAVAAFEDQDYFEQTCQAVIASREQL
                     VSELQDLGFEVLPSAANFIFTRHPQHDAAQLAAALREQGVIVRHFKQERINQFLRITI
                     GTAEQNMALQEALTALVQ"
     gene            complement(4282..5589)
                     /gene="hisD"
                     /locus_tag="VIO83_05650"
     CDS             complement(4282..5589)
                     /gene="hisD"
                     /locus_tag="VIO83_05650"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY8330792.1"
                     /translation="MTAPIAIRRLDAADPQFARHLDHLLSWESVSDEAVNQRVLDIIK
                     GVRERGDAAVVEYTQRFDAVAATSMAELILPRERLELALTRISAEQRVALEKAAERVR
                     LYHERQKQDSWTYTEADGTVLGQQVTPLDRAGLYVPGGKAAYPSSVLMNAIPAKVAGV
                     AEVVMVVPTPRGELNELVLAAACVAGVDRVFTIGGAQAVAALAYGTESVPQVDKIVGP
                     GNIYVATAKRHVFGQVGIDMIAGPSEILVVCDGQTDPDWIAMDLFSQAEHDEDAQSIL
                     VSPDAAFLDKVAESIARLLPTMERAEIIRTSLEARGALIQVTDMEQAIVVANRIAPEH
                     LELSVADPQQWLPQIRHAGAIFMGRYTAEALGDYCAGPNHVLPTSGTARFSSPLGVYD
                     FQKRSSIIFCSADGASELGKTASVLARGESLTAHARSAEYRIK"
     gene            complement(5783..6415)
                     /gene="hisG"
                     /locus_tag="VIO83_05655"
     CDS             complement(5783..6415)
                     /gene="hisG"
                     /locus_tag="VIO83_05655"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY8330793.1"
                     /translation="MLTIALSKGRILDDTLPLLAEAGIVPTENPDKSRKLIIPTTQAD
                     VRLLIVRATDVPTYVEHGAADLGVAGKDVLLEYGGQGLYEPLDLQIAQCKLMTAGKVG
                     APEPSGRLRVATKFVSVAKRYYAEQGRQVDIIKLYGSMELAPLVGLADKIIDVVDTGN
                     TLRANGLEPQELICHVSSRLIVNKASMKMQHARIQALIDTLRQAVESRRH"
     gene            complement(6425..7690)
                     /gene="murA"
                     /locus_tag="VIO83_05660"
     CDS             complement(6425..7690)
                     /gene="murA"
                     /locus_tag="VIO83_05660"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY8330794.1"
                     /translation="MDKLIITGGAPLDGEIRISGAKNSALPILAATLLASEPVTVRNL
                     PHLHDITTMIELFGRMGVEPVINEKLDVEVDATTIKTLVAPYELVRTMRASILVLGPM
                     VARFGQAEVALPGGCAIGTRPVDLHIRGLEAMGAVIDVDGGYIKARAPEGGLRGAHFC
                     FDTVSVTGTENILMAATLANGRSVLENAAREPEVVDLANMLIAMGAKIQGAGTSTITV
                     DGVKRLGGASYAVMPDRIETGTFLVAAAASRGRVKLKDTDPTILEAVLLKLQEAGAEI
                     TTGSDWIELDMKGKRPKAVNVRTAPYPAFPTDMQAQFIAMNAVAEGTGTVIETIFENR
                     FMHVLEMQRMGAQIQVEGNTAIVTGVPRLKAAPVMATDLRASASLVIAALVADGDTLI
                     DRIYHIDRGYECIEEKLQLLGAKIRRVPG"
     gene            complement(7713..7952)
                     /locus_tag="VIO83_05665"
     CDS             complement(7713..7952)
                     /locus_tag="VIO83_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEY8330795.1"
                     /translation="MQAEDVKQHLEAQLPGTRVEVEGQGCDFQLNLISDELAALSPVK
                     RQQQVYAHLNEWIANGSIHAVTMKFFTSTAWAERA"
     gene            complement(8054..8362)
                     /locus_tag="VIO83_05670"
     CDS             complement(8054..8362)
                     /locus_tag="VIO83_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY8330796.1"
                     /translation="MSRGTLREVQPGGLALQGVIDMHVGAQLRDAARRLLAGSPTQEV
                     WIDCAAITQSSSVGLSLLLCLLRDAQATGKRLQIRNLPEDMRQVAEVYGLLELLPLAD
                     "
     gene            complement(8359..9009)
                     /locus_tag="VIO83_05675"
     CDS             complement(8359..9009)
                     /locus_tag="VIO83_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8330797.1"
                     /translation="MYKFLRRGLLVLLACLPFMAHAAATPQQVVQGTTDKVLGELSSK
                     REQFKQDPEAFYLALNSILTPVIDFDGFARGVMTVRYSRQATPEQMQSFQESFKRSLV
                     KFYGSALLEYDNQEIRMLPAPPAREPGRASVNMEVVGRTGAIYPLTYTLEQKGGDWKV
                     RNVIINGINVGKLFRDQFAESMRSNGNDLDKVIATWGDAVARAQETANAQQGGSQQ"
     gene            complement(9032..9508)
                     /gene="mlaD"
                     /locus_tag="VIO83_05680"
     CDS             complement(9032..9508)
                     /gene="mlaD"
                     /locus_tag="VIO83_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEY8330798.1"
                     /translation="MQSRSLEIGVGLFLLAGLLALLLLALRVSGLSVGAAEDTYRLYA
                     YFDNTAGISPRAKVTMAGVTIGKVVTVDLDRDSFMGRVTMELDHRVDNLPVDSTASIL
                     TAGLLGEKYIGISIGGEEEVLKEGGVIRDTQSALVLEDLIGKFLLNSVNKEPAPAK"
     gene            complement(9508..10305)
                     /gene="mlaE"
                     /locus_tag="VIO83_05685"
     CDS             complement(9508..10305)
                     /gene="mlaE"
                     /locus_tag="VIO83_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEY8330799.1"
                     /translation="MRKTPPLERVRLMGRSGLDVLEALGRSTLFLVHALFGRNGAGSF
                     LQLLLKQLYAVGVLSLAIIIVSGIFIGMVLALQGYNILVDFASEEAVGQMVALTLLRE
                     LGPVVTALLFAGRAGSALAAEIGNMKSTEQLSSLEMIGVDPLKYIIAPRLWAGFISMP
                     LLSAIFTVVGIWGGAMVAVDWLGVYEGSYWGNMQRSVEFQGDVLNGVIKSMVFAVVVT
                     WIAVFQGYDCEPTSEGISRATTRTVVYASLAVLGLDFILTALMFGDF"
     gene            complement(10305..11114)
                     /locus_tag="VIO83_05690"
     CDS             complement(10305..11114)
                     /locus_tag="VIO83_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8330800.1"
                     /translation="MSSDNAYAVELKGVTFKRGARTIFDNVDLVFPRGKVTGIMGPSG
                     CGKTTLLRLIGAQLRPSSGAVVVNGQNLPDLSRSELFDMRKQMGVLFQSGALFTDLDV
                     FENVAFPLRVHTDLPEEMIRDIVLMKLQAVGLRGAFELMPDELSGGMKRRVALARAIA
                     LDPQILMYDEPFVGQDPIAMGVLVRLIRLLNDALGLTSIVVSHDLAETASIADYLYVV
                     GDGQVLGQGTPDELMDSDNPRIRQFMKGIADGPVPFHYPAADYRRDLLGDA"
     gene            11307..12293
                     /locus_tag="VIO83_05695"
     CDS             11307..12293
                     /locus_tag="VIO83_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY8330801.1"
                     /translation="MPASMPQSPSFIQSAQHTIRLEIEAVNDLLARINGDFIRACELL
                     LACKGRVVVVGMGKSGHIGRKIAATLASTGTTAFFVHPAEASHGDMGMITRDDVVLAL
                     SNSGSTAEIVTLLPLIKRMGITLISMTGNPDSPLAQAAAVNLDASVRQEACPLNLAPT
                     SSTTVSLVLGDALAIALLEARGFTAEDFAFSHPGGALGRRLLLKVENIMHSGADLPQV
                     QRDTPLREALLEMTRKGLGMTVVTDADGHLAGIFTDGDLRRALDRGVDVRQARIDELM
                     TVGGKTARPDMLAAEALKIMEDHKISALVVIGADNRAIGALNMHDLLRAGVM"
     gene            12294..12818
                     /locus_tag="VIO83_05700"
     CDS             12294..12818
                     /locus_tag="VIO83_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8330802.1"
                     /translation="MNSDLQQRASLIRLAIFDVDGVLTDGKLYFLPEGGEFKTFNTLD
                     GHGIKMLINSGVRTAIISGRKTPVVERRAQNLGIQHLYQGREDKLVVLDELLSELGLS
                     YEQVAYLGDDLPDLPVIRRVGLGMAVASADAFVRQHAAGVTQARGGEGAAREFCELIM
                     RAQGTLEAAQAAYL"
     gene            12829..13398
                     /gene="lptC"
                     /locus_tag="VIO83_05705"
     CDS             12829..13398
                     /gene="lptC"
                     /locus_tag="VIO83_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY8330803.1"
                     /translation="MNRTLRTYLLFAITATTLVVIGYWGIRPGSFMESPATSGTDSAI
                     DFYVENARSVQFQADGKLRYRMTAEHLEHIQSSDVTLVTRPDLLLHRGSDYPWHVQSA
                     RGEVSPGGTQVELIDDVRVARTDAKGRPTIMTSSRLTVFPERNYAETRQAVRIEAANG
                     VTTAVGMQAYLNDGRMLLQSNVRGQHEVR"
     gene            13385..13933
                     /gene="lptA"
                     /locus_tag="VIO83_05710"
     CDS             13385..13933
                     /gene="lptA"
                     /locus_tag="VIO83_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEY8330804.1"
                     /translation="MRFVNPLPLLFGLSTLLAAIPAWALPTDREQPIRVQADSAELDD
                     AKGVAVYRGGVVITQGTLKITGDTVTITQDKNGDIEVFTSVGKPAYYEQKPAEDKPVV
                     KAYGLTIQYFAVNERIVLIDQAKVIQEGNTFEGEKIVYDTQRQIVNAGRATGSNVTTP
                     RPRIDMMIQPRAKKGQAEQGAQ"
     gene            13930..14655
                     /gene="lptB"
                     /locus_tag="VIO83_05715"
     CDS             13930..14655
                     /gene="lptB"
                     /locus_tag="VIO83_05715"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY8330805.1"
                     /translation="MSTLIAQHLAKSYKGREVVRDVSLSIDSGQIVGLLGPNGAGKTT
                     CFYMIVGLVRADQGRILIDEQDVTHLPMHGRARAGIGYLPQEASIFRKLTVADNIMAI
                     LETRKDLDRSARREALESLLQEFHISHIRDNLGMSLSGGERRRVEIARALASDPKFIL
                     LDEPFAGVDPISVGDIKQIIHHLKSRGIGVLITDHNVRETLDICETAYIVNAGQLIAE
                     GDAESILANELVKEVYLGQEFRL"
     gene            14772..16268
                     /locus_tag="VIO83_05720"
     CDS             14772..16268
                     /locus_tag="VIO83_05720"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY8330806.1"
                     /translation="MKHSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALDSN
                     PMLERQEEGDDFDNSDPLAESEPSHSTASHDNTVSTSSERNPDSLDEADQWGERIPSE
                     LPVDTAWEDIYQTSASSLPVSEDDEWDFTARTSAGESLHSHLLWQLNLAPMSDSDRTI
                     AMSIIDAINNDGYLDESLEEILESLDPELDIELDEVEMVLHRVQQFEPLGIGARDLSE
                     CLLLQLNQLPASTPWLDEARRVARDHLDLLGSRDYSQLMRRLRVKEDDLRQIVELIQR
                     LNPRPGSQIETSEAEYVVPDVIVRKDNERWLVELNPDAMPRLRVNPHYAGLVRRADTS
                     ADNTYMRNQLQEARWFIKSLLSRNETLMKVATQIVELQRGFLEYGEEAMKPLVLHDIA
                     EAVGMHESTISRVTTQKYMHTPRGIFELKYFFSSHVSTSEGGECSSTAIRAIIKKLVA
                     AENPKKPLSDSKIAGLLEAQGIQVARRTIAKYRESLGIAPSSERKRLV"
     gene            16349..16657
                     /gene="raiA"
                     /locus_tag="VIO83_05725"
     CDS             16349..16657
                     /gene="raiA"
                     /locus_tag="VIO83_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY8330807.1"
                     /translation="MQVNISGQHLEVTDTLRDYINEKLERLARHFDKITNVQVIMQVE
                     KLQQKIEATLHVAGGEVVANAEHTDMYAAIDLLADKLDRQLIKHKEKLVERGQGTATR
                     "
     gene            16668..17132
                     /gene="ptsN"
                     /locus_tag="VIO83_05730"
     CDS             16668..17132
                     /gene="ptsN"
                     /locus_tag="VIO83_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEY8330808.1"
                     /translation="MIRLEHILTPGRSQVNVPGGSKKRVLEQIANLVARDLPGLDAHD
                     IFENLVAREKLGSTGFGNGIAIPHCRLAGCSVPVCALLRLDVPVDFDALDGAPVDLLF
                     VLLVPATATEEHLELLRQIAGLLDRADVRERLRQAKDGFDLYQVVLDTVDGR"
     gene            17136..17993
                     /gene="rapZ"
                     /locus_tag="VIO83_05735"
     CDS             17136..17993
                     /gene="rapZ"
                     /locus_tag="VIO83_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY8330809.1"
                     /translation="MRMIIVSGRSGSGKSTALAVLEDNGFYCIDNLPASLLPDLAERA
                     LLHTELSQPQVAVSVDARNLPSQLKRFPELLEQLRQHNIQCDVLYLDADNATLLKRFS
                     ETRRRHPLSSPSRSLSEAIDDETVLLGPIADLADLKIDTANLNLYQLRDTIKLRLLDK
                     PEAGTAFLLESFGFKRGMPVDADLVFDVRCLPNPYWKPDLREHSGLEQPVRDYLDALG
                     DVEEMYQDIFSYLNKWLPRFAASNRAYVTVAIGCTGGHHRSVYLAERLGAALRANLGN
                     VQIRHRDLP"
     gene            18013..18285
                     /locus_tag="VIO83_05740"
     CDS             18013..18285
                     /locus_tag="VIO83_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY8330810.1"
                     /translation="MPACDITIINKLGLHARAAAKFVGVAGRFPCQVRVGRSADSLVD
                     GKSIMAVMMLAAGKGTAIHLHTEGEQEQEALQALVELIDNYFDEGE"
     gene            complement(18398..19747)
                     /gene="pmbA"
                     /locus_tag="VIO83_05745"
     CDS             complement(18398..19747)
                     /gene="pmbA"
                     /locus_tag="VIO83_05745"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEY8330811.1"
                     /translation="MNSAVESVGPQALPGLQEQVEAILAEARRQGATDCEVAVSLEQG
                     LSTSVRQGEVETVEFNRDRGFGITLYLGQRKGSASTSAQGAAAIRETVAAALAIAQHA
                     SADDCAGLADAALMARELPDLDLYHPWAITPEQAIEQALACEAAAFARDARVTKADGT
                     TLNTHLGCRVYGNSHGFVGGYASSRHSLSCVMIAEGDGQMQRDYWYDVSRIGSELASA
                     ESIGRRAAERAVGRLGARPVPTCEVPVLFAAELATGLFGHLLAAISGGNLYRKSSFLE
                     GALGQQLFPDWLSLDERPHLPRGMGSASFDGDGLATYAKPFVADGKLVSYALGTYSGR
                     KLGLPSTANAGGVHNLFVSHGAEDQAALIRRMDRGLLVTELMGQGLNLVTGDYSRGAA
                     GFWVENGVIQFPVQEVTIAGNLRDMFKGIVAVGSDVETRGNVRSGSVLIDKMMLGGS"
     gene            19842..20363
                     /gene="yjgA"
                     /locus_tag="VIO83_05750"
     CDS             19842..20363
                     /gene="yjgA"
                     /locus_tag="VIO83_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEY8330812.1"
                     /translation="MSEQFEDDFSEEKSKSQVKRELHALQDLGERLTTLKQDMLNRLP
                     LSDALLKALIEAPKHKAHAARKRHIQYIGKLMRDQDIDAIVALLDQMDSSTRQYNERF
                     HALERWRDRLVGGTDADLEALLAEYPEADVQHLRQLIRHAQHEAARDKPPAASRKVFK
                     YLRDMDEVRRGLR"
     gene            complement(20442..21881)
                     /gene="tldD"
                     /locus_tag="VIO83_05755"
     CDS             complement(20442..21881)
                     /gene="tldD"
                     /locus_tag="VIO83_05755"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEY8330813.1"
                     /translation="MNTLSSVSEQLLTPGGLGLERLPALLGELVGPGIDAADLYFQSQ
                     VSESWMLEDGIVKEGSFHLDQGVGVRAQSGEKTGFAYSNSIEEHALRQAIGAARSIAR
                     AGQQGRVHTPSVQVPPRLYGADNPLEVLSRAEKVELLKRIDQATRALDSRISRVTVSL
                     AGVWEQVLVAATDGSLGADVRPLVRFNVSVIVEHNGRRERGAHGGGGRTDYRYFLEND
                     RAMDYAREAVRQALLNLEAIPAPAGSMPVVLGNGWSGVLLHEAVGHGLEGDFNRKGSS
                     AYSGRVGQQVASSLCTIVDDGTLPGRRGSLSLDDEGTPTQCTTLIENGVLKGYMQDKL
                     NARLMGVQPTGNGRRESYAHLPMPRMTNTYMLAGESDPEEIIRSVKRGLYCASLGGGQ
                     VDITSGKFVFSTSEAYLIEDGRITAPVKGATLIGNGPEAMSRVSMVGNDLALDSGVGT
                     CGKDGQSVPVGVGQPTLKIEAITVGGTGG"
     gene            complement(21878..22732)
                     /locus_tag="VIO83_05760"
     CDS             complement(21878..22732)
                     /locus_tag="VIO83_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY8330814.1"
                     /translation="MGTVAVIQMVSQSDVLANLARARVLLEQAAAGGAQLAVLPENFA
                     AMGRRDLAVLGMVEAAGEGPLLPWLEATARELGLWIVAGTLPLPPQGQRDGKPCACSL
                     LFDSAGQVAARYDKLHLFDVEVADSRGSYRESDDFAHGEHLVLADTPVGRLGLTVCYD
                     LRFAELYSALREAGAELISAPSAFTAVTGAAHWEVLVRARAIETQCYLLAANQGGQHP
                     GPRETFGHSLIVDPWGQPLAREARGEAVLLAQIDRPAQAAIRQRMPVSAHRRLRPGAP
                     QPLTKESS"
     gene            complement(22778..>23044)
                     /locus_tag="VIO83_05765"
     CDS             complement(22778..>23044)
                     /locus_tag="VIO83_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEY8330815.1"
                     /translation="STRLELDGQLDMAHDRIAAKLRVTLPLSNNLPLAALLAGAAPVA
                     GALLIVDQLVGKHLSRVASVEYRVDGPWQDPKISLFGRPSEDAR"
BASE COUNT         4063 a         7920 c         7364 g         3697 t
ORIGIN      
        1 cgacatgatg cggaaacctt agaagtagcc ctgacgtttc ttttcttcca tggaaccggc
       61 gccatcgtgg tcgatgacgc ggccctggcg ctcggaagtg cgggtcagga gcatttcctg
      121 gatgatctcg ggcaggctgg cagccgtgga ctccaccgcg ccggtcagcg ggtagcagcc
      181 gagggtacgg aagcgcacca tcttcttctg gatgcgcgcc ttctcttcat cgctgagatg
      241 ctcgaggatg cgcgcatcgt cgatcatgat cagggtgccg tttttctcga tcacttcgcg
      301 ctcggcggcg aagtacagcg gcacgatcgg gataccttcc aggtagatgt actgccagat
      361 gtccagctcg gtccagttgg acagcgggaa cacgcggatc gactcgccct tcttgacctt
      421 gccgttgtag acgttccaca gctcgggacg ctggttcttc gggtcccagc ggtgcttgct
      481 gtcgcggaac gaatagacgc gctccttggc ccgcgacttc tcctcgtcgc ggcgcgcacc
      541 accgaaggcg gcgtcgaagc cgtacttgtc cagagcctgc ttgaggccct cggtcttcat
      601 gatgtcggtg tgcttggcgc tgccgtgggt gaacgggttg atgccctgcg ccacgccttc
      661 cgggttgacg taggtgatca ggtccaggcc cagctcggcg accatcttgt cgcggaagcg
      721 gtacatctcc tggaacttcc actgggtatc gacgtgcatc accgggaacg gcagcttgcc
      781 gggaaagaag gccttgcggg ccagatgcag catgacggca gaatccttgc cgatggagta
      841 cagcatcacc gggttatcga actcggcggc cacctcgcgg atgatgtgga tgctttccgc
      901 ctccagctgc ttcagatgcg tcagtttgtc gaccatggct actcacaaga gaacaaaggc
      961 ggcctgcggg ccgcggacgt tggcgcacga taccatgcgg accttcttct aataaggcgc
     1021 caacatagac tcaatcattc tataaatata cccaagcgct tgcgctcaca ccgggttggc
     1081 gcagtcgatg aagcggtgtt cgaggccgaa gcgctgcgcc agccactcgc ccagcgcctg
     1141 cacgccgtag cgctcggtgg cgtgatggcc ggcggcgaga aagctgatgc cgttctcgcg
     1201 cgcgctgtgc acggtggact cggatacctc gccgctcagg taggcatcca cgcccgcggc
     1261 gatcgcctgc tcgatatagc cctgcgccgc accggtgcac caggccaccc ggcggatcgg
     1321 cccgcagcca ggcaccagca gcggctcgcg gccgagcacc tgcgccacct gggcggcaaa
     1381 ctccgccggg ctgagcgcct gcgccaggct gccgagcagg ccgaccgaac gcggattgcc
     1441 cggttccagc ggcccttcca cctgcaggcc aaggcgcctg gccagctgca cgttgttgcc
     1501 gacctcggga tgctgatcca gcggcaggtg gtaggccagc aggctgatgt catggctcag
     1561 cagggtcttc agccggcgct gcttcatccc ggtgatgcag gcattctcgt tcttccagaa
     1621 atagccgtgg tgcaccagca ccagatcggc ctgcgcttcg accgcggcat ccagcagcgc
     1681 ctggctggcg gtcacgccgc tgacgatgcg gcgcacctcg gcgcgcccct cgacctgcag
     1741 gccgttgggg caatagtcga cgatgcgcgc ggcatccaga taacgatcgg tttcagcaac
     1801 cagggtgtgc agggcgacag ccatgagact ctccgtaaca gtcggcgact gcttagcgtg
     1861 cgccgaactc gtataatgcg gccaccttaa ttggccctcc cccgccctcg caagcccaag
     1921 gacccccctg atgtacaagg cactgcgcta cctcggctgg ccggttgtcg ccggaatcct
     1981 gctggccctg ctggcgatcc agttcttccc gcaatgggtc ggccacggcc agccggatct
     2041 gcaactgcgc caggcgccag ccctcggcct gcccagcctc gggccggtgt cctacggcga
     2101 cgcggtggag ctggccgcac cggcggtggc caacctgtac accaccaagg tggtggacaa
     2161 gccgagccac ccgctgttcg gcgacccgca attccgccgc ttcttcggcg acaacctgcc
     2221 gcgccagcgc cgcatggaat ccagcctcgg ctcggcggtg atcatgagcc cgcgaggcta
     2281 cctgctgacc aacaaccatg tcaccgccgg cgccgaccag atcatcgtgg cactcaagga
     2341 tggccgtgaa accctggccc gagtaatcgg cagcgacccg gaaaccgacc tggcggtgct
     2401 gaagatcgac ctgcccgacc tgccggcgat caccctcggc cgctccgaca acatccgcat
     2461 cggcgacgtc gccctggcca tcggcaaccc gttcggcgtc ggccagaccg tgaccatggg
     2521 catcatcagc gccaccgggc gcaaccagct cggcctcaac acctacgagg acttcatcca
     2581 gaccgacgcg gcgatcaacc cgggcaactc cggcggcgcg ttgatcgacg cccacggcaa
     2641 cctgatcggc atcaacaccg cgatcttctc ccgctccggc ggctcgcagg gcatcggctt
     2701 cgccatcccg accaagctgg cccaggaggt gatggaggcc atcatcaacc acggccaggt
     2761 gatccgcggc tggctcgggc tcgaggtgca accactgacc acggagctgg ccgaatccat
     2821 cggcctgcag ggccaggccg gcatcatcgt cgccggcatc taccgggaca gtccggccct
     2881 gcatgccggc ctgcaacccg gcgacgtgat cctggccatc gacggcgagc cggccagcga
     2941 cggccgccgg gcgatgaacc aggtggcgcg caagcgtccc ggcgaaaagc tgcacatcga
     3001 cattctgcgc gccggcgaga agcacgcgct caaggccgaa gtcggcctgc gcccgccggt
     3061 cgccagcgag tgagaccact gccttgatag aacgaaaaac ggcgcccgcg ggcgccgttt
     3121 ttcgttgtcc agtgctgccc gccggtcact gcaccagcgc ggtcagcgcc tcctgcaggg
     3181 ccatgttctg ctcggcggta ccgatggtga tgcgcaggaa ctgattgatc cgctcctgct
     3241 tgaagtggcg cacgatcacg ccctgctcgc gcagggctgc cgccagctgc gcggcgtcat
     3301 gctgggggtg gcgggtgaag atgaagttgg ccgccgacgg cagcacctcg aagcccagat
     3361 cctgcagctc gctgaccagc tgctcgcggc tggcgatcac cgcctggcag gtctgctcga
     3421 agtagtcctg gtcctcgaac gccgccaccg cgccgaccag ggccaaacgg tccaacggat
     3481 aggagttgaa gctgttcttg atccgctcca gcgcctcgat caactcggca ttgccgaccg
     3541 ccaggccgac gcgcaggccc gccagcgaac gcgacttgga cagggtctgg gtgaccagca
     3601 ggttcggata ctggtcaacc aggctgatcg cgctctcgcc gccgaagtcg acataggcct
     3661 cgtcgaccac caccaccgac tcggtgttgg cttccagcag ccggcggatc gccgccagcg
     3721 gcagcaggca accggtcggg gcattcgggt tggggaagat gatgccgccg ttggggcgct
     3781 cgtagtccgc tacattgatg cggaactgca catccagcgc cacggtttcg aacgggatgt
     3841 cgtacaggcc gcagtacacc gggtagaagc tgtagctgat atccgggaac agcagcggcc
     3901 ggccgtgctg gaacaggccg tggaacacgt gggccagcac ctcgtcggag ccattgccga
     3961 gaaacacctg gctggcctgc acgccatagt agtcggcgat ggtctgcttc aggcgctcgc
     4021 cgttggggtc cgggtacagg cgcaggctgt cgtccagctc ggcgcggatc gcctcgagca
     4081 ccttgggcga cgggccgtag gggttttcgt tggtgttcag tttgatcagc cgcgccatct
     4141 tcggctgctc acccggcaca tagggcacca ggtccttgac gaaggggctc cagaatttgc
     4201 tcacgccctt ctctcctcga atgcgtaggg tgcgcggtgc gcaccgaatc gttccagggt
     4261 gcgcatggcg caccctacgg cttacttgat gcggtactcg gcgctgcgcg cgtgggcggt
     4321 cagcgactcg ccgcgcgcca gtaccgaggc ggtcttgccc agctcggagg cgccgtcggc
     4381 cgaacagaag atgatcgacg agcgcttctg gaagtcgtag acccccagcg gcgaggagaa
     4441 gcgcgcggtg ccggaggtcg gcagcacgtg gttcggcccg gcgcagtagt cgcccagcgc
     4501 ctcggcggta tagcggccca tgaagatcgc gccggcgtgg cggatctgcg gcagccactg
     4561 ctgcgggtcg gccaccgaca gctcgaggtg ctcgggagcg atgcggttgg cgacgacgat
     4621 ggcctgctcc atgtcggtga cctggatcag cgcgccgcgg gcttccagcg aggtacggat
     4681 gatctcggcg cgctccatgg tcggcagcag acgggcgatg ctctcggcca ccttgtcgag
     4741 gaaggccgca tccggactga ccaggatcga ctgggcgtcc tcgtcgtgct cggcctggga
     4801 gaacaggtcc atggcgatcc agtccggatc ggtctgaccg tcgcagacca ccaggatctc
     4861 cgaggggccg gcgatcatgt cgatgccgac ctgaccgaac acgtggcgct tggcggtggc
     4921 cacgtagatg ttgcccggac cgacgatctt gtccacctgc ggcacgctct cggtaccgta
     4981 ggccagcgcg gccacggcct gggcgccgcc gatggtgaac acgcggtcga cgccggccac
     5041 gcaggcggcg gccagcacca gttcgttgag ttcgccgcgc ggcgtcggca ccaccatcac
     5101 cacctcggcg acgccggcga ccttggccgg aatggcgttc atcagtaccg acgacggata
     5161 ggcggccttg ccgcccggca cgtacaggcc ggcacgatcc agcggggtga cctgctggcc
     5221 gagcacggtg ccgtcagcct cggtgtaggt ccaggagtcc tgcttctgcc gctcgtggta
     5281 caggcgcacg cgctcggcgg ccttttccag cgccacgcgc tgctcggcac tgatgcgggt
     5341 cagcgccagc tccaggcgct cgcgcggcag gatcagttcg gccatggagg tcgccgcgac
     5401 ggcgtcgaag cgctgggtgt actcgaccac cgccgcatcg ccgcgctcgc gcactccctt
     5461 gatgatgtcc agcacgcgct ggttgaccgc ttcgtcggaa acgctttccc agctcagcag
     5521 atgatccaga tggcgagcaa actgcggatc ggcggcgtcg agtcggcgga tggcgattgg
     5581 tgcggtcatg gcaaagcctc tctggcgtaa gcggggtcgg gcgccgctag actagcaggc
     5641 cttccgtgcg ggcacccggc aattgtggct atgacacgga taggcaatcg cgaccggtgg
     5701 tcgcaggaca aaacgggatg ttcggcatct gtcggcgaac gccgaccgat gcgggcgccg
     5761 tccgtggcgc cgccgaggac actcagtgcc ggcgtgactc cacggcctgg cgcagggtat
     5821 cgatcagcgc ctggatacgc gcgtgctgca tcttcatcga ggccttgttg acgatcagcc
     5881 gcgagctgac gtggcagatc agctcctgcg gttccagacc gttggcgcgc agggtgttgc
     5941 cggtgtcgac cacgtcgatg atcttgtcgg ccagacccac cagcggcgcc agctccatgg
     6001 aaccgtacag cttgatgatg tccacctggc ggccctgctc ggcgtagtag cgcttggcca
     6061 cgctgacgaa cttggtggcc acgcgcaacc ggccgctggg ctcgggggcg ccgaccttgc
     6121 cggcggtcat cagcttgcac tgggcgatct gcaggtccag cggctcgtag agcccctggc
     6181 cgccgtactc cagcagcaca tccttgccgg ccacgccgag atcggcggcg ccgtgctcga
     6241 cgtaggtcgg cacatcggtg gcgcgcacga tcagcaggcg tacatcggcc tgggtggtcg
     6301 gaatgatcag cttgcggctc ttgtccggat tctcggtcgg cacgatgccg gcttccgcca
     6361 gcagcggcag ggtatcgtcg agaatgcggc ccttggacag cgcgatggtc agcatgactg
     6421 gttcctagcc cggcacacgg cgaatcttgg cgccgaggag ctgcagcttt tcctcgatgc
     6481 actcgtagcc acggtcgatg tggtagatgc ggtcgatcag ggtgtcgcca tcggcgacca
     6541 gggcagcgat caccagactg gccgacgcgc gcaggtcggt ggccatcacc ggggcggcct
     6601 tgaggcgcgg tacgccggtg acgatggcgg tattgccctc gacctggatc tgcgcgccca
     6661 tgcgctgcat ttccagcacg tgcatgaagc ggttctcgaa aatggtctcg atcaccgtgc
     6721 cggtgccttc ggccaccgca ttcatggcga tgaactgcgc ctgcatgtcg gtggggaacg
     6781 ccgggtaggg tgcggtccgc acgttgaccg ccttcggccg cttgcccttc atgtccagct
     6841 cgatccagtc gctgccggtg gtgatctcgg cgccggcttc ctgcagcttg agcaacacgg
     6901 cctcgaggat ggtcggatcg gtatccttga gcttgacccg gccacggctg gcggcagccg
     6961 ccaccaggaa ggtgccggtc tcgatgcggt cgggcatcac cgcgtagctg gcgccaccca
     7021 ggcgcttgac gccgtcgacc gtgatggtcg aagtgccggc gccctggatc ttggcgccca
     7081 tggcgatcag catgttggcc aggtcgacca cttccggctc gcgtgcggcg ttctcgagca
     7141 cgctgcggcc gttggccagg gtggccgcca tcaggatgtt ctcggtgccg gtcacgctga
     7201 cggtatcgaa gcagaagtgc gcgccacgca ggccgccttc cggggcacga gccttgatgt
     7261 agccgccatc gacgtcgatg accgcgccca tggcctcgag cccgcggatg tgcaggtcga
     7321 ccggacgagt gccgatggca cagccgccgg gcagtgcgac ttcggcctgg ccgaagcggg
     7381 cgaccatcgg gccgagcacc aggatcgagg cacgcatggt cctgaccagc tcgtagggcg
     7441 ccaccagggt cttgatggtg gtggcatcga cctcgacgtc aagcttctcg ttgatcaccg
     7501 gctctacgcc catgcgaccg aacagctcga tcatggtggt gatgtcgtgc aggtgcggca
     7561 ggttgcgcac ggtgaccggc tcggaagcca gcagggtggc ggcgaggatc ggcaacgccg
     7621 agttcttcgc accggaaata cggatttccc cgtcgagcgg ggcgccgccg gtaatgatca
     7681 atttgtccat tgggagtctc ggtcgccggc gctcaggcgc gctcggccca ggcggtgctg
     7741 gtgaagaatt tcatggtcac ggcgtggatg ctgccattgg cgatccactc gttgagatga
     7801 gcgtatacct gctgctgacg cttgaccggg ctgagggcag ccagctcgtc gctgatcagg
     7861 ttcagctgga agtcgcaacc ctgcccctcg acttccacgc gggtgccggg cagctgcgct
     7921 tccagatgct gtttgacgtc ttccgcctgc ataactcaac ctcaatgggg aaccagcatg
     7981 atttggccgg tcatcataca aaaaagcccc cgacctgcga accccgcgcg cgccacggcg
     8041 cccggcagca gcctcagtcg gcgagaggca acagctcgag cagaccgtag acctcggcga
     8101 cctgacgcat gtcctccggc aggttacgga tctgcagacg cttgccggtc gcctgcgcat
     8161 cacgcagcag gcacagcagc agcgacaggc cgacgctgct gctctgggtg atggcggcac
     8221 agtcgatcca cacctcttgg gtgggactgc cggccagcag acggcgggcc gcatcacgca
     8281 attgggcgcc gacatgcatg tcgatcaccc cttgcagcgc cagaccgccg ggctgcacct
     8341 cgcgcagcgt gcctcgactc attgctggct gccgccctgc tgggcattgg cggtttcctg
     8401 ggcgcgcgcc acggcgtcac cccaggtggc aatcaccttg tccagatcgt tgccgttgct
     8461 gcgcatcgac tcggcgaact ggtcgcggaa cagcttgccg acgttgatgc cgttgatgat
     8521 cacgttgcgc accttccagt cgccgccctt ctgctccagg gtgtaggtca gcgggtagat
     8581 ggcgccggta cggccgacca cttccatgtt caccgaagcc cgacctggct cgcgggcggg
     8641 cggcgccggc agcatgcgga tttcctggtt gtcgtactcg agcagggcac tgccgtagaa
     8701 cttcaccagg ctgcgcttga agctttcctg gaaggactgc atctgctccg gcgtagcctg
     8761 gcggctgtag cgcacggtca tcacgccgcg cgcgaagccg tcgaaatcga tcaccggcgt
     8821 gaggatgctg ttgagcgcca ggtagaaggc ctcgggatcc tgtttgaact gctcgcgctt
     8881 gctgctcagc tcgcccagca ccttgtcggt ggtgccttgc accacctgct gcggagtggc
     8941 cgcagcgtga gccatgaagg gcaggcaggc cagcagcacc agcaggccac gacgcaggaa
     9001 cttgtacatg gtggtttcct atcttgcttg tttacttggc gggggcgggt tctttgttga
     9061 cggagttgag caggaacttg ccgatcaggt cttccagcac cagtgccgac tgggtatcgc
     9121 ggatcactcc gccctccttc agcacctcct cttcgccacc gatactgatg ccgatgtact
     9181 tctcgccgag caggccggcc gtgaggatcg acgcggtgga gtcaaccggt agattgtcga
     9241 cgcgatgatc gagttccatg gtcacccgcc ccatgaagct gtcgcggtcc agatccaccg
     9301 tcaccacctt gccgatggtc accccggcca tggtcacctt ggcgcgcggg ctgataccgg
     9361 ccgtgttgtc gaagtaggca tacaggcgat aggtatcttc cgccgcaccg acgctcaggc
     9421 cactgacgcg cagggccagc agcagcaggg ccagcagtcc ggccagcagg aacaggccaa
     9481 caccgatttc cagagaacgc gattgcatca aaaatctcca aacatcagag cggtaagaat
     9541 gaagtcaagg ccgagcacgg ccagcgaggc gtagaccacg gtgcgggtag tggcgcgact
     9601 gatgccctcg gaggtcggtt cgcaatcgta gccctggaac accgcgatcc aggtcaccac
     9661 cacggcgaac accatgctct tgatcacgcc gttgagcaca tcgccctgaa actccacgct
     9721 gcgctgcatg ttgccccagt aggagccctc gtagacgccg agccagtcga cggcgaccat
     9781 cgcgccgccc cagatgccga ccacggtgaa gatcgccgag agcagcggca tggaaatgaa
     9841 gccggcccac aggcgtggcg cgatgatgta cttgagagga tcgacgccga tcatctccag
     9901 gctggacaac tgttcggtgg acttcatgtt gccgatttcg gcggccagcg ccgaaccggc
     9961 gcgaccggcg aacagcagcg cggtcaccac cggccccagc tcgcgcagca gggtcagcgc
    10021 caccatctgc ccgaccgcct cctcggaggc gaagtcgacc aggatgttgt agccctgcag
    10081 ggccagcacc atgccgatga agatgccgga aacgatgatg atcgccagcg acagcacgcc
    10141 gaccgcatac agctgcttga gcagcaactg caggaagctg ccagcgccgt tgcgcccgaa
    10201 cagggcatgc acgaggaaca gggtcgagcg gccgagggcc tcgagcacat cgagaccgga
    10261 acgccccatc aggcgcaccc gctcgagtgg cggagtcttg cgcatcaggc gtctcccagc
    10321 aggtcgcggc gatagtcggc cgcaggatag tggaagggca ccggaccgtc ggcgatgccc
    10381 ttcatgaact ggcggatgcg cggattgtcg gagtccatca gctcatccgg cgtgccctgc
    10441 cccagcacct ggccgtcgcc gaccacgtac aggtagtcgg cgatgctggc ggtttccgcc
    10501 aggtcgtggg aaaccacgat gctggtcagg ccgagcgcgt cgttgagcag gcggatcagc
    10561 cgcaccagca cgcccatggc gatgggatcc tggccaacga acggttcgtc gtacatgagg
    10621 atctgcgggt cgagggcgat tgcccgagcc agcgccacgc ggcgcttcat gccgccagac
    10681 agctcgtcgg gcatcagctc gaaggcgcca cgcaggccga ccgcctgcag cttcatcagc
    10741 acgatgtcgc ggatcatctc ctcgggcagg tcggtgtgca cccgcagcgg gaaggcgacg
    10801 ttctcgaaca cgtcgagatc ggtgaacagg gcgccgctct ggaacagcac gcccatctgc
    10861 ttgcgcatgt cgaacagttc gctgcgcgac aggtccggca ggttctggcc attgaccacc
    10921 acagcaccgc tggacgggcg caactgcgcg ccgatcaggc gcagcagcgt ggttttcccg
    10981 cagccggaag gccccatgat gccagtcacc ttgccgcggg gaaaaaccag atctacgtta
    11041 tcgaagatgg tgcgcgcgcc gcgcttgaag gttacacctt tcagttcgac ggcatacgca
    11101 ttgtcgctac tcatcggtac tccttgcgtg tcggcctgcg ggatggacgt ctcgcgccac
    11161 gctggagaca ctcgccgggc ataagccgaa ttgccgcgca ctatagcacc gccgcccgaa
    11221 cgcacccaag cgcccgccgc gctgccaagc gcagatgatg agtgatcggc actttatcgc
    11281 tataatggcc gtccactcag ccgaccatgc ctgcctccat gccccagtcg ccctccttta
    11341 tccaatcggc ccagcacacc atccgcctgg agatcgaggc tgtgaacgat ctgctggcac
    11401 ggatcaacgg cgacttcatc cgcgcctgcg agctgctgct ggcctgcaag gggcgcgtgg
    11461 tggtggtcgg catgggcaag tccggccata tcggccgcaa gatcgccgcc accctggcca
    11521 gcaccggcac cacggcgttt ttcgtccatc cggccgaggc cagccacggc gacatgggca
    11581 tgatcacccg cgacgatgtg gtgctggcgc tgtccaactc cggcagcacc gccgagatcg
    11641 tcaccctgct gccgttgatc aagcgcatgg gcatcaccct gatcagcatg accggcaatc
    11701 ccgactcgcc gctggcccag gccgccgcgg tcaatctgga tgccagtgtc cggcaggaag
    11761 cctgcccgct gaacctcgcg ccgacctcct ccaccaccgt gtcactggtg ctcggcgacg
    11821 ccctggccat cgccctgctc gaggcccgcg gcttcacggc ggaagacttc gccttctccc
    11881 acccgggcgg cgcgctgggg cggcgcctgc tgctcaaggt ggagaacatc atgcacagcg
    11941 gcgccgacct gccgcaggtg cagcgcgaca ccccgctgcg cgaggccctg ctggaaatga
    12001 cccgcaaggg tctgggcatg accgtggtca ccgacgccga cggccacctg gccggcatct
    12061 tcaccgacgg cgacctgcgc cgcgccctcg accgcggggt cgacgtgcgc caagcgcgca
    12121 tcgacgagct gatgaccgtc ggcggcaaga cggcccgccc cgacatgctc gcggccgaag
    12181 cgctgaagat catggaagac cacaagatca gcgcactggt ggtgatcggt gcagacaacc
    12241 gcgccatcgg cgcgctgaac atgcacgacc tgctgcgtgc cggggtaatg tgaatgaata
    12301 gcgacttgca gcaacgtgcc agcctgatcc gtctggcaat cttcgatgtc gacggcgtgc
    12361 tgaccgacgg caagctgtat ttcctgcccg aaggcggcga attcaagacc ttcaacaccc
    12421 tcgacggcca cggcatcaag atgctgatca actccggcgt gcgcaccgcg atcatcagcg
    12481 gccgcaagac cccggtagtc gagcggcggg cgcagaacct cggcatccag catctctacc
    12541 agggccgcga ggacaagctg gtggttctcg atgaactgct gagcgaactg ggactctcat
    12601 atgaacaagt cgcctatctg ggcgacgacc tgccggacct gccggtaatc cgccgggtcg
    12661 gcttgggcat ggcagtagcc agcgccgacg ccttcgtccg ccagcacgcc gccggcgtga
    12721 cccaggcccg cggtggcgaa ggcgccgccc gcgagttctg cgagctgatc atgcgcgccc
    12781 agggcacgct ggaggcagcc caggctgcct acctgtaacg ggacatacat gaatcgcacg
    12841 ctgcgcacct atctgctgtt cgccatcact gccaccacgc tggtcgtcat cggctactgg
    12901 ggcatccgcc cgggaagctt catggagtct ccggcaacct cgggaaccga cagcgccatc
    12961 gacttctacg tcgaaaacgc acgcagcgtg cagttccagg ccgatggcaa gctgcgttac
    13021 cggatgaccg ccgagcacct cgagcacatt cagagcagcg acgtcaccct ggtcacccgt
    13081 ccagacctgc tgctgcatcg cggcagcgac tatccttggc acgtgcagag cgcacgcggc
    13141 gaggtgtcgc ccggcggcac ccaggtcgaa ttgatcgatg acgtccgcgt ggcacgcacc
    13201 gacgccaagg gccgcccgac catcatgacc agcagccggc tgaccgtatt cccggagcgg
    13261 aattacgccg agacccgcca ggccgtgcgc atcgaggctg ccaatggcgt caccacggct
    13321 gtcggtatgc aggcctatct caacgacggc aggatgctcc tgcagtccaa cgtacgagga
    13381 cagcatgagg ttcgttaacc cccttcccct tctgtttggt ctgagcaccc tgctggccgc
    13441 catcccggcc tgggccctgc ccaccgaccg cgagcaaccg atccgcgtgc aggccgacag
    13501 cgccgagctg gacgatgcca agggcgtggc cgtgtaccgc ggcggcgtgg tgatcaccca
    13561 gggcacgctg aagatcaccg gcgacaccgt gaccatcacc caggacaaga acggcgacat
    13621 cgaggtattc acctcggtcg gcaagccggc ctactacgag cagaaacccg ccgaggacaa
    13681 gccggtggtc aaggcctacg gcctgaccat ccagtacttc gccgtcaacg agcgcatcgt
    13741 cctcatcgac caggccaagg tcatccagga aggcaacacc ttcgagggcg agaagattgt
    13801 ctatgacacc cagcgccaga tcgtcaacgc cgggcgcgcc acgggcagca acgtgaccac
    13861 gccgcggccg cgcatcgaca tgatgatcca gccgcgcgcc aagaagggtc aggcggagca
    13921 aggcgcgcaa tgagcaccct gattgcccag cacctggcca agagctacaa ggggcgtgaa
    13981 gtcgtgcgcg acgtcagcct ttcgatcgac agcgggcaga tcgtcggcct gctcggcccc
    14041 aacggcgccg gcaagaccac ctgcttctac atgatcgtgg gcctggtacg cgccgaccag
    14101 gggcggatcc tgatcgacga gcaggacgtc acccatctgc cgatgcacgg ccgcgcccgc
    14161 gccggcatcg gctacctgcc gcaggaagcc tcgattttcc gcaagctgac ggtggccgac
    14221 aacatcatgg cgatcctcga gacccgcaag gatctcgacc gcagcgcgcg ccgcgaggcc
    14281 ctggaaagcc tgctgcagga gttccatatc agccacatcc gcgacaacct cggcatgagc
    14341 ctgtccggcg gcgagcggcg acgggtggaa atcgcccgcg ccctggccag cgacccgaag
    14401 ttcatcctgc tcgacgagcc ctttgccggc gtcgacccga tctcggtcgg cgacatcaag
    14461 cagatcatcc accacctcaa gagccgcggc atcggtgtgc taatcaccga ccacaacgta
    14521 cgcgagaccc tggatatctg cgagacggcc tatattgtca acgcgggcca gctgatcgcc
    14581 gaaggcgatg ccgagagcat cctcgccaac gagctggtca aggaagtcta cctcggccag
    14641 gagttcaggc tttaagagac taggcaaagc cgcgcgcctc gggcatataa tttgctaaac
    14701 gaggcgctcc ggcgcccagc agtggacagc gcgcaggcgc tggcagaccc caaggtgcca
    14761 actccccagc catgaaacac tcgctagtcc taaaaatggg ccagcagctg acgatgactc
    14821 ctcagctgca gcaagccatt cgtctgctcc aactgtcgac cctcgatctg cagcaggaaa
    14881 tccaggaagc cctcgactcg aacccgatgc tcgagcgcca ggaagagggc gacgacttcg
    14941 acaacagcga tccgctggcg gaaagcgagc catcccactc gacggccagt cacgacaaca
    15001 ccgtcagcac cagcagcgaa cgcaaccccg acagcctcga cgaggccgac cagtggggcg
    15061 agcgcatccc cagcgaactg ccggtggata ccgcctggga agacatctac cagaccagtg
    15121 ccagcagcct gccggtcagc gaggacgacg agtgggactt caccgcccgc acctccgccg
    15181 gcgaaagcct gcacagccac ctgctctggc agctcaacct ggcaccgatg agcgacagcg
    15241 accgcacgat cgccatgtcg atcatcgatg ccatcaacaa cgacggctac ctcgacgaaa
    15301 gcctcgagga gatcctcgag tccctcgacc ccgagctgga catcgagctg gacgaagtgg
    15361 agatggtcct gcatcgcgtg cagcagttcg agccgctggg catcggcgcg cgcgacctca
    15421 gcgagtgcct gctgctgcaa ctcaaccagc tgccggccag cacgccctgg ctggacgaag
    15481 cccgccgcgt ggcgcgcgac cacctcgacc tgctcggcag ccgtgactac agccagctga
    15541 tgcgacgcct gcgggtcaag gaagatgacc tgcgccagat cgtcgagctg atccagcgcc
    15601 tcaacccgcg tccgggctcg cagatcgaga ccagcgaagc ggaatacgtg gtccccgacg
    15661 tgatcgtgcg caaggacaac gagcgctggc tggtcgagct caaccccgat gccatgccgc
    15721 gcctgcgcgt caacccacac tacgccggtc tggtacgccg cgccgacacc agcgccgaca
    15781 acacctacat gcgcaaccag ctgcaggagg cgcgctggtt catcaagagt ctgctcagcc
    15841 gcaacgagac gctgatgaag gtcgccacgc agatcgtcga gctgcagcgc ggcttcctcg
    15901 agtacggcga ggaggcgatg aagccgctgg tcctgcacga catcgccgag gcggtgggca
    15961 tgcacgagtc gaccatctcg cgggtgacca cgcagaaata catgcacacc ccgcgcggca
    16021 tcttcgagct caagtacttt ttctccagcc acgtcagcac gtctgaaggc ggcgagtgct
    16081 cgtccacggc gatccgcgcc atcatcaaga aactggtcgc agcggaaaac ccgaaaaagc
    16141 cattgagcga cagcaagatc gctggtttac tggaggcaca agggattcag gtggcccgcc
    16201 ggaccatcgc caagtatcgg gaatccttgg gcattgcgcc ctcgagcgaa cgcaagcgac
    16261 tggtgtgacg ctcgagcaaa aacaacatcg cattccaact gaccgggccc gcccctgtcg
    16321 gtattcgcag gtaacaagga gaagcagcat gcaagtcaac atcagtggcc aacacctgga
    16381 agtgactgac actctgcgcg actacatcaa cgagaaactg gagcgcctgg cgcgccactt
    16441 cgacaagatc accaacgtcc aggtgatcat gcaggtggaa aaactgcagc agaaaatcga
    16501 ggcgaccctg cacgtcgccg gtggcgaagt ggtcgccaac gccgagcata ccgacatgta
    16561 cgccgccatc gacctgctgg ccgacaagct ggaccgccaa ctgatcaagc acaaggaaaa
    16621 gctggtcgag cgcggacagg gcactgccac ccgctgatcc cttcccgatg attcgactag
    16681 agcacatcct gacccccggc cgttcccaag tgaacgtgcc gggaggcagc aagaagcgcg
    16741 ttctcgaaca gatcgccaac ctcgtagccc gggatcttcc cgggctcgac gcacatgaca
    16801 tcttcgaaaa cctggtagcc cgggaaaagc tcggttccac cggcttcggc aacggcatcg
    16861 ccattcccca ctgccggctg gccggctgct cggtgccggt atgcgccctg ctgcgcctgg
    16921 atgtaccggt ggacttcgac gccctcgacg gggcgccggt ggacctgctg ttcgtcctgc
    16981 tggtgccggc aaccgccacc gaggagcacc tcgagctgct gcgccagatt gccggcttgc
    17041 tcgaccgtgc cgacgtgcgc gaacgcctgc gccaggcgaa ggatggcttc gacctctacc
    17101 aggtggtgct cgataccgtg gacgggcgct gagccatgcg catgatcatt gtcagcgggc
    17161 gctcggggtc ggggaaaagc accgccctgg cagtgctcga ggacaacggc ttctactgca
    17221 tcgacaacct gccggccagt ctgctgccgg atctggccga acgcgccctg ctgcataccg
    17281 agctgtcgca accgcaggtc gccgtgtcgg tcgacgcgcg caacctgccc agccagctca
    17341 agcgctttcc cgagctgctg gaacagctgc gccagcacaa catccagtgc gacgtgctgt
    17401 atctcgacgc cgacaatgcc accctgctca agcgcttctc ggaaacccgc cgccgccacc
    17461 cgctgagcag cccaagccgc tcgctgagcg aggcgatcga cgacgaaacc gtcctgctcg
    17521 gcccgatcgc cgacctggcc gatctgaaga tcgacaccgc caacctcaac ctgtaccagc
    17581 tgcgcgacac catcaagctg cgcctgctgg acaagccgga ggccggcacc gccttcctgc
    17641 tcgaatcctt cggcttcaag cgcggcatgc ccgtggacgc cgacctggtg ttcgacgtgc
    17701 gctgcctgcc caacccctac tggaaaccgg acctgcgtga gcacagcggc ctggaacaac
    17761 cggtacgcga ctatctggat gccctgggcg acgtcgagga gatgtatcag gacatcttca
    17821 gctacctgaa caagtggctg ccgcgctttg ccgccagcaa ccgcgcctac gtcaccgtgg
    17881 ccatcggctg caccggtggc catcaccgct cggtgtatct ggccgagcgc cttggcgctg
    17941 ccctgcgcgc caacctcggc aacgtacaga tccgccaccg cgatctcccc tgacaggaca
    18001 gtccccaccg cgatgccagc ctgcgacatc accatcatca acaagctcgg cctgcatgcc
    18061 cgtgccgccg ccaagttcgt cggcgtggcc ggccgcttcc cctgccaggt ccgcgtcggc
    18121 cgctcggcag acagcctggt ggacggcaag agcatcatgg cggtgatgat gctcgccgcc
    18181 ggcaagggca ccgctatcca cctgcatacc gaaggcgagc aggagcagga ggccttgcag
    18241 gccctggtcg agctgatcga caactacttc gacgaaggcg aataaactcg ccgacgcgcc
    18301 aagggctggc gcagggatgg acaacccgaa gcgcgggttt ccacccatgc ctcaacagcg
    18361 gaaaagggcc tgtggccgtt ttccgccgtc ttgtccctca gctgccgccc agcatcatct
    18421 tgtcgatcag caccgagccg ctgcgtacgt tgccgcgcgt ttccacatcg ctgcccaccg
    18481 cgactatgcc cttgaacatg tcgcgcaggt tgccggcgat ggtgacttcc tgcaccggga
    18541 actggatcac cccgttctcc acccagaagc ccgccgcacc gcgcgagtag tcgccggtga
    18601 ccagattcag cccctgcccc atcagctcgg tcaccagcag gccacggtcc atgcgacgga
    18661 tcagtgcggc ctgatcctca gccccgtggc tgacgaacaa gttgtgcacg ccaccggcgt
    18721 tggccgtgct gggcaggccc agcttgcgtc cggagtaggt gcccagcgcg taggacacca
    18781 gcttgccgtc cgcgacgaag ggcttggcgt aggtagccag gccgtcgccg tcgaaactgg
    18841 cgctgcccat gccgcgcggc aggtgcgggc gctcgtcgag gctcagccag tcggggaaca
    18901 gctgctggcc cagcgcgccc tcgaggaagg aggacttgcg gtacaggttg ccaccggaga
    18961 tcgccgccag cagatggccg aacaggccgg tggccaactc ggcggcgaac agcaccggca
    19021 cctcgcaggt cggcaccggt cgcgcgccca ggcgaccgac cgcacgttcg gcggcgcgcc
    19081 ggccgatcga ctcggcgctg gccagctcgc tgccgatacg gctgacgtcg taccagtagt
    19141 cgcgctgcat ctgcccgtcg ccctcggcaa tcatcacgca gctcaggctg tgccggctgg
    19201 aggcatagcc gccgacgaaa ccgtggctgt tgccgtacac ccggcagccc aggtgggtat
    19261 tgagggtggt gccgtcggcc ttggtgacgc gcgcatcacg ggcgaaggcc gccgcctcgc
    19321 aggccaaggc ctgctcgatg gcctgctccg gggtgatcgc ccacggatgg tagaggtcca
    19381 agtccggcag ctcgcgcgcc atcagcgcgg catcggccaa gccggcgcag tcgtccgcgg
    19441 aagcatgctg ggcgatcgcc agcgctgccg ccacggtttc gcggatcgcc gccgcgccct
    19501 gcgccgaggt gctcgccgag cccttgcgct ggccgagata gagagtgatg ccgaagccgc
    19561 ggtcgcggtt gaactccacc gtctccacct cgccctggcg cacgctggtg gacagcccct
    19621 gctccagcga caccgccacc tcgcagtcgg tcgcgccctg gcgacgcgcc tcggcgagga
    19681 tcgcctcgac ctgctcctgc aaccccggca gggcctgcgg ccccacgctt tccacggctg
    19741 aattcatgtc ctgctcctgt tttcccgccg gctcgccggc cgggccggac aagcggcccc
    19801 tgactggtta tcatgacggc gtttcccagc ggacccgccc catgtctgaa caattcgaag
    19861 acgacttctc cgaggagaag agcaaatccc aggtcaagcg cgaactgcat gcgctgcagg
    19921 atctgggtga acgcctgacc accctcaagc aggacatgct caaccgcctg ccgctgagcg
    19981 atgcgctgct caaggcgctg atcgaggcgc cgaaacataa ggcgcacgcg gcgcgcaaac
    20041 gccacatcca gtacatcggc aagctgatgc gcgaccagga catcgatgcc atcgtcgccc
    20101 tgctcgacca gatggactct tccacccggc agtacaacga acgcttccac gccctcgagc
    20161 gctggcgcga ccgcctggtc ggcggcaccg acgccgacct cgaagcgctg ctcgccgagt
    20221 accccgaggc cgacgtccag cacctgcgcc agctgatccg ccacgcccag cacgaggccg
    20281 cccgcgacaa accgccagcc gcctcacgca aggtattcaa gtacctgcgc gacatggacg
    20341 aggtcaggcg cggcttgcgc tgagtcccct gccgtctcag gagcgaattc attcgcgacc
    20401 ttctgccggt cgcagatgaa tctgctccta cagaccgtcc cttacccccc ggtcccaccg
    20461 acggtgatcg cctcgatctt cagggtcggc tggccgacgc ccaccggcac cgactggccg
    20521 tccttgccgc aggtgccgac gccgctgtcc agcgccaggt cgttgccgac catcgacacc
    20581 cggctcatcg cctccggtcc gttgccgatc agggtggcgc ccttcaccgg cgccgtgatc
    20641 cggccatcct cgatcaggta ggcctcgctg gtggagaaca cgaacttgcc gctggtgatg
    20701 tccacctgtc cgccgccgag gctggcgcag tacaggccgc gcttcaccga gcggatgatc
    20761 tcctccgggt cgctctcgcc ggccagcatg taggtgttgg tcatgcgcgg catcggcagg
    20821 tgcgcatagg actcgcggcg accgttaccg gtcggctgca cgcccatcag gcgggcgttg
    20881 agcttgtcct gcatgtagcc cttgagcacg ccgttttcga tcagcgtggt gcactgggtc
    20941 ggcgtaccct cgtcgtcgag gctcagcgag ccgcgacgcc ccggcagggt gccgtcatcg
    21001 acgatggtgc acaggctcga ggccacctgc tggccgaccc ggccgctgta ggccgagctg
    21061 cccttgcggt tgaagtcgcc ctccaggccg tggccgaccg cctcgtgcag cagcacgccg
    21121 ctccagccgt tgccgagcac caccggcatg ctgccggccg gcgccggaat cgcttccagg
    21181 ttgagcagcg cctggcgcac cgcctcgcgg gcgtagtcca tggcgcggtc gttctccagg
    21241 aagtagcggt agtcggtacg cccgccaccg ccgtgggcgc cgcgttcgcg gcgaccgttg
    21301 tgctcgacga tcacgctgac gttgaagcgc accagcggac gcacgtcggc gcccaggctg
    21361 ccgtcggtgg ccgccaccag cacctgctcc cagaccccgg cgaggctcac cgtgacccgc
    21421 gagatgcgcg agtccagcgc gcgggtggcc tggtcgatgc gcttgagcag ttcgaccttc
    21481 tccgcccggc tgagcacctc cagcggattg tcggcgccgt acaggcgcgg cggcacctgc
    21541 accgaggggg tgtgcacgcg gccctgctgg ccggcgcggg cgatcgagcg cgccgcgccg
    21601 atggcctggc gcagggcgtg ttcctcgatg ctgttactgt aggcaaagcc ggtcttttcg
    21661 ccggactggg cgcgcacgcc gacgccctgg tcgaggtgga agctgccttc cttgacgatg
    21721 ccgtcctcga gcatccacga ctcggacacc tggctctgga agtacaggtc ggcggcatcg
    21781 atgccgggcc cgaccagctc gccgagcagc gccggcaggc gctccaggcc gaggccgccg
    21841 ggggtcagca actgctcgct gacggatgac agggtgttca tgaagactcc ttggtcaagg
    21901 gttgcggcgc gcccgggcgc agacggcgat gggcgctgac cggcatgcgc tggcggatcg
    21961 ccgcctgcgc cgggcggtcg atctgtgcca gcagcacggc ttcgccacgc gcctcgcggg
    22021 ccagcggctg gccccaggga tcgacgatca gcgaatggcc gaaggtttcc cgcggcccgg
    22081 gatgctggcc gccctggttg gcggccagca gatagcactg ggtttcgatg gcccgcgcgc
    22141 gcaccagcac ctcccagtgc gccgcgccgg tcaccgcggt gaacgccgag ggcgcgctga
    22201 tcagctcggc gccggcttcg cgcagggcgc tgtacagttc ggcaaagcgc agatcgtagc
    22261 agaccgtcag gcccaggcgc cctaccggcg tatcggccag caccaggtgc tcgccgtggg
    22321 caaaatcgtc ggactcgcgg tagctgccgc ggctgtcggc cacctcgacg tcgaacagat
    22381 gcagcttgtc gtagcgcgcg gccacctggc cagcgctgtc gaacagcagc gagcaggcgc
    22441 agggcttgcc gtcgcgttgc ccttgcggcg gcagcggcag ggtgcccgcg actatccaca
    22501 ggcccagctc gcgcgccgtg gcttccagcc agggcagcag cggcccctcg ccggcagcct
    22561 cgaccatgcc gagtacggcc agatcgcgcc gtcccatggc cgcaaagttt tccggcagga
    22621 cggccaactg cgccccgccc gcggcggcct gctcgagcag cacccgcgcc cgcgccagat
    22681 tggcgaggac gtcggactga ctgaccatct ggatgactgc caccgtgccc attgctatct
    22741 cctgccgcgt aacagtctca tcctaacccg gatgggccta gcgtgcgtcc tccgacggcc
    22801 tgccgaacag gctgatcttc gggtcctgcc aaggcccgtc gacccgatac tcgacgctgg
    22861 ccacccgcga caggtgcttg cccaccagtt gatcgacgat caacagcgcc cccgccaccg
    22921 gcgccgcgcc ggccagcagc gccgccagcg gcaggttgtt ggacagcggc agggtcaccc
    22981 gcagcttggc ggcgatgcgg tcatgggcca tgtccagctg gccatccagc tccaggcggg
    23041 tgga
//
LOCUS       DATCUN010000140         9105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_5631, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000140 DATCUN010000000
VERSION     DATCUN010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9105
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5631"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2554
                     /locus_tag="VIO83_05770"
     CDS             <1..2554
                     /locus_tag="VIO83_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEY8330816.1"
                     /translation="EQTPAAAPASGEPQAAPLASDNGGRALASRSADYSHLDWVPREK
                     LSEAQRAEIAPYCAGTYVEPQRPGIDDTTPQGEAPTFVGARASRYEQQTETTTLAGDV
                     VLRQGSMQAEADEASLHQQENRGELKGNVRLRDNGILVVGDRAQLQLDNGEAEVDNAE
                     YVIHAAKTRGSAKYAKRQDDAVVRLKDGTYTRCEPGENTWHLKGNNIKLDPATGFGTA
                     TNVTLRVKDVPVFYTPYIYFPIDDRRQSGFLTPSMSTSSDSGFSLKTPYYINLAPNYD
                     ATLYPTYMANRGLLMEGEFRYLTPASEGQVGGAWLNDAEDERKLQSEYEDQRWMYNWQ
                     HKGGLDSRWLAEIDYTDISDPYYFQDLDTDLGIDRPDFLNQRGSLSYRGDSYTARLNV
                     HAYEPTSVVDVTPYDRLPQLTLDGALPFQPGGLQFSYGTEFVSFQRSLRNGNFINEDG
                     LAQPWYDNNLRGLDRAEGDRLHLEPGVSLPLNWSWGYLKPSLKYAYTRYDLDLDQQGR
                     DSLLAEQQFDSSQDRQIPIYSVDSGLYFDRNTDLFGKGTRQTLEPRAFYLYVPEEDQT
                     DIPVFDTGENTFSYASLWRDNRFSGKDRIGDANQLSLGATSRWIEPNGFERQTISLGQ
                     TFYFEDRQVQMPGIDYRTRGDARTSTSPYALEYLYRFNRDWRFNLDLAQERSLGQTRS
                     GSAMFHYQPEANPNKVVNLGYRYRNDAMRYDQASGTWTYGGDFGTPGTPGYIKDYYKI
                     QQHDFSTIWPIVPQWSVIARWQHDYSRNRTLEAMGGFEYDSCCWKLRLINRYWMDYDE
                     YDLDPAANEEADRGIFLQIVFKGLGGVVGSQVESFLDEGIQGYREREDQAYQ"
     gene            2532..3827
                     /gene="surA"
                     /locus_tag="VIO83_05775"
     CDS             2532..3827
                     /gene="surA"
                     /locus_tag="VIO83_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HEY8330817.1"
                     /translation="MKTKLINSLLPLALGALLCCSAAQAEVQPLDRVVAIVDNDVIMQ
                     SQLDQRLDEVRQNLAKRGGDLPPRDALAPQVLERLIVENLQLQMGERSGIRIGDEELN
                     RAMATIAQRNNLSLEQFRAALARDGLSYDDAREQVRREMVVSRVRQRRVAERIQVSPQ
                     EVQNFLASDLGKLQLSEEFRLANILVPLSEGSSAQAIQAAERQARDIHTQLQQGADFA
                     RLAMARSASENALEGGEIGWRKAAQLPPPFDAMVGALGVGEVTEPVRTPAGFIMLKLL
                     EKRGGERGVQRDEVHVRHILLKPSEIRSDDETRMLAERLYERLQGGEDFAELAKSFSE
                     DPGSALNGGDLNWIDPNSLVPEFREVMASSPVGRLSQPFRSPFGWHVLEVLDRRTSDA
                     SGAAREQQALNLLRNRKYDEELQNWLRQIRDEAYVEIRL"
     gene            3824..4816
                     /gene="pdxA"
                     /locus_tag="VIO83_05780"
     CDS             3824..4816
                     /gene="pdxA"
                     /locus_tag="VIO83_05780"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY8330818.1"
                     /translation="MTEARRFALTPGEPAGIGPDLCLLLARAAQPHALVAIASRPLLA
                     ERATQLGLDIALLDVGPGAWPQTPAPAGSLYVWDTPLAAPVHAGQLDPRNAGYVLETL
                     LRAGNGCVNGDFAGMITAPVHKGVINEAGIPFSGHTEFLAKRTHTEQVVMMLATRGLR
                     VALVTTHLPLREVADAITAERLARVARILHGDLHNKFGIANPRILVCGLNPHAGEGGH
                     LGREEIDIIEPTLARLRAEGLDLIGPLPADTLFTPKHLEHCDAVLAMYHDQGLPVLKY
                     KGFGAAVNVTLGLPIVRTSVDHGTALDLAGTGKIDTGSLQVALETAWQMTGQRD"
     gene            4932..5738
                     /gene="rsmA"
                     /locus_tag="VIO83_05785"
     CDS             4932..5738
                     /gene="rsmA"
                     /locus_tag="VIO83_05785"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009685237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY8330819.1"
                     /translation="MSELYQHRARKRFGQNFLHDAGVIHRILRAIHARPGEHMVEIGP
                     GQGALTEGLLGSGAKLDVIELDKDLLPLLKMRFGLDPNFTLNAGDALKFDFNSLAADG
                     EKLRVVGNLPYNISTPLIFHLLEHAAIIGDMHFMLQKEVVERLAATPGGGDWGRLSIM
                     VQYHCRVEHLFNVGPGAFNPPPKVESAIVRLVPHAVLPHPAKDHRLLERVVREAFNQR
                     RKTLRNTLKQLLDAQAIEAAGVDGSLRPEQLDLAAFVRLADQLALQTAAE"
     gene            5778..6164
                     /gene="apaG"
                     /locus_tag="VIO83_05790"
     CDS             5778..6164
                     /gene="apaG"
                     /locus_tag="VIO83_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEY8330820.1"
                     /translation="MTSNDPRYQIDVSVVTRHLPEQSLPEQGRYAFAYTVTIRNAGLL
                     AAKLLTRHWVITDGNAKVQEVRGPGVVGEQPLLQPGQSHTYTSGCVLATPVGSMHGSF
                     GMQAGDDHAFAAPIKPFRLAVPGALH"
     gene            6164..6988
                     /locus_tag="VIO83_05795"
     CDS             6164..6988
                     /locus_tag="VIO83_05795"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEY8330821.1"
                     /translation="MTLYAVGDVQGCLQPLQCLLQRVAFDPQHDRLWLVGDLVNRGPQ
                     SLETLRFLFAMREAVTTVLGNHDLHLLAVAHNIERLKKGDTLREILDAPDRDDLLDWL
                     RRQPLLHHDDERQITLVHAGIPPHWSLKKARKRAGEVEEALRDDTRLPLFLEGMYGNE
                     PNKWDSRLRGVARLRTITNYFTRMRFCTADGRLDLKSKEGLGTAPSGYAPWFSHPGRK
                     TRGQKIIFGHWAALQGQCDEPGVEALDSGCVWGATLTLLNVDSGERLTCSCHKEQP"
     gene            6985..7314
                     /gene="glpE"
                     /locus_tag="VIO83_05800"
     CDS             6985..7314
                     /gene="glpE"
                     /locus_tag="VIO83_05800"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HEY8330822.1"
                     /translation="MSEFKRIDPATAHDLRQHGAVVVDIRDPHSFASGHIPGARHLDN
                     HSLADFIAGADMDKPLLVICYHGNSSQNAAGFLAHQGFAEVYSLDGGFELWRAQYPDE
                     VARLPHD"
     gene            7555..>9105
                     /locus_tag="VIO83_05805"
     CDS             7555..>9105
                     /locus_tag="VIO83_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEY8330823.1"
                     /translation="MSIFSHFQQRYETTRQEEYSLQEYLDLCKQDPLTYASAAERMLK
                     AIGEAQLVDTASDPRLSRIFSNKVIRRYPAFADFHGMEDCIDQIVSYFRHAAQGLEEK
                     KQILYLLGPVGGGKSSLAEKLKHLMEHIPFYAIKGSPVFESPLGLFNPDEDGAILEEE
                     YGIPQRYLSSIMSPWATKRLNEFGGDISKFRVVKLYPSILNQIAIAKTEPGDENNQDI
                     SALVGKVDIRKLEEFPQNDPDAYSYSGALCRANQGIMEFVEMFKAPIKVLHPLLTATQ
                     EGNYNSTEGLGAIPYSGILLAHSNEAEWHSFRNNKNNEAFIDRIYIVKVPYCLRVTDE
                     IKIYDKLLVNSSLAHAHCAPDTLKMLAQFTVLSRLKEPENSNIYSKMRVYDGENLKDT
                     DPKAKSIQEYRDAAGVDEGMNGLSTRFAFKILSKVFNFDPHEVAANPVHLLYVLEQQI
                     EQEQFPAETRERYLRFLKEYLAPRYVEFIGKEIQTAYLESYSEYGQNIFDRYVLYADF
                     WIQDQEYRD"
BASE COUNT         1715 a         3285 c         2769 g         1336 t
ORIGIN      
        1 cgagcaaacg ccggccgcag ccccggcgag cggtgagccg caagcggcgc cgttggccag
       61 cgacaatggc ggccgggccc tggcttcgcg cagcgccgac tacagccacc tggactgggt
      121 gccgcgcgag aaactcagcg aggcccagcg cgccgagatc gccccctact gcgccggcac
      181 ctacgtcgag ccacaacggc ccggcatcga cgacacaacg ccgcagggcg aggcgccgac
      241 cttcgttggt gcccgcgcct cgcgctacga gcagcagacc gaaaccacca ccctcgctgg
      301 cgacgtggtc ctgcgccagg gcagcatgca ggccgaagcc gacgaggcca gcctgcacca
      361 gcaagaaaac cgcggcgaac tgaagggcaa cgtgcgcctg cgcgacaacg gcatcctggt
      421 agtcggcgac cgcgcccagc tgcagctgga caacggcgag gccgaagtcg acaacgccga
      481 gtacgtgatc cacgcggcca agacccgcgg cagcgcgaaa tacgccaagc gccaggacga
      541 cgccgtggtg cgcctgaaag acggcaccta cacgcgctgc gaaccgggcg aaaacacctg
      601 gcacctgaag ggcaacaaca tcaagctgga cccggccacc ggcttcggca ccgcgaccaa
      661 cgtgaccctg cgggtgaagg atgtaccggt gttctacacc ccgtacatct acttcccgat
      721 cgacgaccgt cgccagtccg gcttcctgac gccgagcatg agcacgtcga gcgactccgg
      781 cttctcgctg aagacgccct actacatcaa cctggcgccc aactatgacg ccaccctcta
      841 tcccacctac atggccaatc gcggcctgct gatggagggc gagttccgct acctgacccc
      901 ggccagcgaa ggccaggtcg gcggagcctg gctgaacgac gcggaagacg agcgcaagct
      961 gcagtcggag tacgaagacc agcgctggat gtacaactgg cagcacaagg gcgggctgga
     1021 ctcgcgctgg ctggccgaga tcgactacac cgacatcagc gatccgtact acttccagga
     1081 cctggatacc gacctcggga tcgaccggcc ggacttcctc aaccagcgcg gcagcctgag
     1141 ctatcgcggc gacagctaca ccgcgcggct caatgtgcat gcctacgagc cgaccagcgt
     1201 ggtcgacgtc accccctacg accgcctgcc acagctgacc ctcgacggcg ccctgccgtt
     1261 ccagccgggc ggcctgcagt tcagctacgg caccgagttc gtcagtttcc agcgcagcct
     1321 gcgcaacggc aacttcatca acgaggatgg tctcgcccag ccctggtatg acaataacct
     1381 cagaggactg gaccgcgccg aaggcgaccg cctgcatctg gaaccgggcg tcagcctgcc
     1441 gctgaactgg tcgtggggct acctcaagcc gtcgctcaag tacgcctaca cccgctacga
     1501 cctggacctc gaccagcagg gtcgcgatag cctgcttgcc gagcagcagt tcgatagcag
     1561 ccaggaccgc cagatcccga tctacagcgt cgacagcggc ctgtatttcg accgcaacac
     1621 cgacctgttc ggcaagggca cccgccagac cctcgagccg cgcgccttct acctgtacgt
     1681 gccggaagag gatcagaccg acattccggt attcgacacc ggcgagaaca ccttcagcta
     1741 tgcctcgctg tggcgcgaca accgcttctc cggcaaggat cgcatcggcg atgccaacca
     1801 gctgtcgctg ggggccacca gtcgctggat cgagcccaat ggtttcgagc ggcagaccat
     1861 cagtctcggt cagaccttct acttcgaaga ccgccaggtg cagatgccgg gcatcgacta
     1921 ccgcacccgt ggcgatgcac gcacctccac ctcgccttat gcactggagt atctgtaccg
     1981 cttcaaccgc gactggcgct tcaaccttga tctcgcccag gaacgctccc tgggccagac
     2041 gcgctccggc agtgccatgt tccactacca gccggaggcc aacccgaaca aggtggtcaa
     2101 cctcggctat cgctaccgca acgacgccat gcgttacgac caggccagcg gcacctggac
     2161 ctatggcggc gacttcggca cccccggcac gcccggctac atcaaggact actacaagat
     2221 ccagcagcac gacttctcca ccatctggcc gatcgtaccg cagtggagcg tgatcgcccg
     2281 ctggcagcac gactacagcc gcaaccgcac gctggaagca atgggcggct tcgagtacga
     2341 cagctgctgc tggaagctgc gcctgatcaa ccgctactgg atggactacg acgaatacga
     2401 cctcgacccg gctgccaatg aggaagccga ccgcggcatc ttcctgcaga tcgtattcaa
     2461 gggcctcggc ggcgtggttg gcagccaggt agagtctttc ctcgatgaag gcattcaagg
     2521 ttaccgtgaa cgtgaagacc aagcttatca atagtctgct ccccctcgcc ctgggcgccc
     2581 tgctgtgctg cagcgcggcc caggccgagg tccagccact cgaccgcgtg gtagccatcg
     2641 tcgacaacga cgtgatcatg cagagccagc tcgaccagcg cctcgacgaa gtgcggcaga
     2701 acctggccaa gcgcggcggc gacctgccgc cacgcgacgc cctggcgccg caggtgctgg
     2761 aacgcctgat cgtcgagaac ctgcagctgc agatgggcga gcgctccggc atccgcatcg
     2821 gcgacgagga actgaaccgg gcgatggcga ccatcgccca gcgcaacaac ctcagcctcg
     2881 agcagttccg tgccgccctc gcccgcgacg gcctgtccta cgacgacgcc cgcgagcagg
     2941 tacgccgcga gatggtcgtc agtcgcgtac gccagcgccg ggtggccgaa cgcatccagg
     3001 tcagcccgca ggaagtgcag aacttcctcg cctccgacct gggcaagctg cagctgtcgg
     3061 aagagttccg cctggccaat atcctcgtgc cgttgagcga gggctcctcg gcccaggcga
     3121 tccaggctgc cgaacgccag gcccgcgaca tccataccca gctgcagcaa ggcgccgact
     3181 tcgcccgcct ggccatggcc cgctcggcca gcgaaaacgc cctcgagggc ggcgagatcg
     3241 gctggcgcaa ggccgcccag ctaccgccgc cgttcgacgc catggtcggc gccctcggcg
     3301 tcggcgaagt caccgagccg gtacgcacgc cggccggctt catcatgctc aagctgctcg
     3361 agaagcgcgg cggtgagcgc ggtgtgcagc gcgatgaagt ccacgtccgc cacatcctgc
     3421 tcaagcccag cgagattcgc agcgacgacg aaacccgcat gctggccgaa cgcctgtatg
     3481 aacgcctgca gggcggcgag gacttcgccg agctggcgaa gagcttctcc gaagacccgg
     3541 gctcggcgct caacggcggc gatctgaact ggatcgaccc caactcgctg gtgcccgagt
     3601 tccgcgaagt gatggccagc agcccggtcg gccgcctgtc gcagccgttc cgcagcccgt
     3661 tcggctggca cgtgctggaa gtgctggatc gccgcaccag cgacgccagt ggcgcggccc
     3721 gcgaacagca ggcgctgaac ctgctgcgca accgcaagta cgacgaggaa ctgcagaact
     3781 ggctgcgcca gatccgcgac gaagcctacg tggaaatccg cctgtgaccg aggcccggcg
     3841 ctttgccctg acccccggcg agccggccgg catcggtccc gacctgtgcc tgctgctcgc
     3901 ccgcgcggcg cagccgcatg ccctggtggc gatcgccagc cgcccgctgc tggccgaacg
     3961 tgccacgcag ctgggcctgg acatcgccct gctcgacgta ggtcccggcg cctggccaca
     4021 aaccccggca ccggccggca gcctttacgt gtgggatacg ccgctggcgg caccggtgca
     4081 cgccggccag ctcgacccgc gcaacgccgg ctacgtgctg gagaccctgc tgcgcgccgg
     4141 caacggctgc gtcaacggcg acttcgccgg gatgatcacc gctccggtgc acaagggcgt
     4201 gatcaacgag gccggcatcc ccttctccgg gcataccgag ttccttgcca agcggaccca
     4261 taccgagcag gtggtgatga tgctcgccac ccgcggcctg cgcgtggcgc tggtgaccac
     4321 ccacctgccg ctgcgcgagg tggccgacgc catcaccgcc gagcgcctgg ctcgggtcgc
     4381 gcgcatcctg catggcgacc tgcacaacaa gttcggcatt gccaacccgc gcatcctggt
     4441 ctgcggcctc aacccgcacg ccggcgaggg cggccacctg gggcgcgagg aaatcgacat
     4501 catcgagccg accctggcgc gcctgcgcgc ggaaggcctc gacctgatcg gcccgctgcc
     4561 ggccgacacc ctgttcaccc ccaagcatct cgagcactgc gacgcggtgc tggccatgta
     4621 ccacgaccag ggcctgccgg tgctcaagta caagggtttc ggcgccgcgg tcaacgtcac
     4681 cctcggcctg ccgatcgtgc gcacttcggt cgaccatggt accgccctgg atctggccgg
     4741 caccggcaag atcgacaccg gcagcctgca ggtagccctg gaaaccgcct ggcagatgac
     4801 cggccagcgc gactgacccc ggcagagccg ccgcctggcg gctgtgctgc gggccggacg
     4861 gggatggcat gcgccaggcg gccagccctg ttaagctttc tgccttctgt tttccgcctc
     4921 cgagtcagcc catgtccgag ctctaccagc accgcgcgcg caagcgtttc ggccagaact
     4981 tcctgcacga tgccggtgtc atccaccgca tcctgcgcgc catccatgcg cgcccgggcg
     5041 agcacatggt ggaaatcggc cccggccagg gcgcccttac cgagggcctg ctcggcagcg
     5101 gcgccaagct ggacgtgatc gagctggaca aggacctgct acccctgctg aagatgcgct
     5161 tcggcctcga cccgaacttc accctcaacg ccggcgatgc cctgaagttc gacttcaaca
     5221 gcctggcggc ggacggcgag aaactgcgcg tggtcggcaa cctgccgtac aacatctcca
     5281 cgccgctgat cttccatctg ctggagcacg ccgcgatcat cggcgacatg cacttcatgc
     5341 tgcagaagga agtggtcgag cgcctggccg ccacgcccgg cggcggcgac tggggacggc
     5401 tgtcgatcat ggtgcagtac cactgccggg tggagcacct gttcaacgtc ggtccgggcg
     5461 ccttcaaccc gccgcccaag gtcgaatcgg ccatcgtccg cctggtgccg cacgcggtcc
     5521 tgccgcaccc ggccaaggat catcgcctgc tcgagcgcgt ggtgcgcgaa gccttcaacc
     5581 agcggcgcaa gaccctgcgc aacaccctca agcagctgct cgacgcccag gccatcgaag
     5641 ccgccggcgt ggatggcagc ctgcgcccgg aacagctgga cctggccgcc ttcgtgcgcc
     5701 tggccgatca gctggccctg cagacagcgg ccgagtaaat cactacgctg tccattacgc
     5761 cgtacgaaac cgccgcaatg acctcaaacg acccgcgcta ccagatcgac gtcagcgtcg
     5821 tcacccgcca cctgcccgag cagtcgttgc ccgagcaggg acgctatgcc ttcgcctaca
     5881 cggtgaccat ccgcaacgct ggcctgctcg ccgccaagct gctgacccgc cactgggtca
     5941 tcaccgacgg caatgccaag gtgcaggaag tgcgcggccc cggcgtggtc ggcgaacagc
     6001 cgctgctgca gcccgggcag agccacacct acaccagcgg ctgcgtgctg gccacgccgg
     6061 tgggcagcat gcacggcagc ttcggcatgc aggccggcga cgaccacgcc ttcgccgcgc
     6121 cgatcaagcc gttccgcctg gccgtgcccg gagccctgca ctgatgacgc tgtatgccgt
     6181 cggcgacgtg cagggctgcc tgcagccact ccagtgcctg ctgcaacggg tcgccttcga
     6241 tccgcagcac gatcgcctgt ggctggtcgg tgacctggtc aaccgcggcc cgcaatcgct
     6301 ggaaaccctg cgcttcctgt tcgccatgcg cgaggcggtg accaccgtgc tgggcaatca
     6361 cgacctgcac ctgctggccg tggcgcacaa catcgaacgg ctgaagaagg gcgacaccct
     6421 gcgcgagatc ctcgacgccc cggatcgcga cgacctgctc gactggctgc gccgccagcc
     6481 gctgctgcat cacgacgacg agcggcagat caccctggtg cacgccggca tcccaccgca
     6541 ctggtcgctg aagaaggccc gcaagcgcgc cggcgaggtc gaggaggcgc tgcgcgacga
     6601 cactcgcctg ccgctgttcc tcgagggcat gtacggcaac gagccgaaca agtgggacag
     6661 ccggctgcgc ggcgttgccc ggctgcgtac gatcaccaac tatttcaccc gcatgcgctt
     6721 ctgcaccgcc gacggccgcc tcgatctgaa gagcaaggaa gggctgggca ccgcgccgag
     6781 cggctatgcg ccgtggttca gccaccccgg ccgcaagacc cgcggccaga agatcatctt
     6841 cggccactgg gcggccctgc agggccagtg cgacgagccc ggcgtcgagg ccctggattc
     6901 cggctgcgtg tggggcgcga cgctgaccct gctcaacgta gacagcggcg agcgccttac
     6961 ctgcagttgc cacaaggaac aaccatgagc gaattcaagc gcatcgaccc cgccaccgcc
     7021 cacgacctgc gccagcatgg cgcggtggtg gtcgacatcc gtgatccgca cagctttgcc
     7081 agcggccaca tccccggcgc ccgccatctg gacaaccact cgctggccga cttcatcgcc
     7141 ggtgccgaca tggacaagcc gctgctggtg atctgctatc acggcaactc cagccagaac
     7201 gcggccggct tcctggccca tcagggcttt gccgaggtct acagcctcga cggcggcttc
     7261 gaactgtggc gcgcccagta tccggacgaa gtcgcccgcc tgccccacga ctgaacgcca
     7321 agacacgctc agcccgtcac cacgccctcg ttttttcctg ctgcgcgctc gacaaagcag
     7381 gtctatgctt ttcccaagag tcttccaaaa gggaaagccg gcccaccggc ttccatagag
     7441 gcggtacgac cattgggggt cgagtgaggg gaccagcagg ttggcgcagc gcgccagcct
     7501 gtccgtgccc gctcgaggcc cgcttccggt tcaggcatcg agcgaggtga cgtcatgagc
     7561 attttcagcc atttccagca acgctacgaa accacgcgcc aggaggaata ctccctgcag
     7621 gagtatctcg acctgtgcaa gcaggatccg ctgacctatg ccagcgccgc cgagcgcatg
     7681 ctcaaggcca tcggtgaggc gcagctggtg gatacggcca gcgacccgcg gctgtcgcgg
     7741 atcttctcca acaaggtgat tcgccgctac ccggcgttcg ccgacttcca cggcatggaa
     7801 gactgcatcg accagatcgt gtcctacttc cgccatgccg cccagggcct ggaagagaag
     7861 aagcagatcc tctacctgct cggcccggtc ggcggcggca agtcgtcgct ggcggagaag
     7921 ctcaagcacc tgatggagca catccccttc tacgccatca agggctcgcc ggtgttcgag
     7981 tcgccgctcg gcctgttcaa cccggacgag gacggcgcca tcctcgagga ggaatacggc
     8041 atcccgcagc gctacctgag ctcgatcatg tcgccgtggg cgaccaagcg cctcaacgag
     8101 ttcggcggcg acatcagcaa gttccgcgtg gtcaagctgt acccgtcgat cctcaaccag
     8161 atcgccatcg ccaagaccga gcccggcgac gagaacaacc aggacatctc cgcgctggtc
     8221 ggcaaggtcg atatccgcaa gctcgaggaa ttcccgcaga acgatccgga tgcctacagc
     8281 tactccggcg cgctgtgccg cgccaaccag ggcatcatgg aattcgtcga gatgttcaag
     8341 gcgccgatca aggtgctgca cccgctgctg accgccaccc aggaaggcaa ctacaacagc
     8401 accgagggcc tcggcgcgat tccctacagc ggcatcctgc tggcccactc caacgaagcc
     8461 gagtggcaca gcttccgcaa caacaagaac aacgaggcgt tcatcgaccg catctacatc
     8521 gtcaaggtgc cctactgcct gcgcgtcacc gacgagatca agatctacga caagctgctg
     8581 gtcaacagct cgctggccca cgcccactgc gcgccggaca ccctgaagat gctcgcccag
     8641 ttcaccgtgc tgagccgcct gaaggagccg gaaaactcca acatctactc gaagatgcgt
     8701 gtctacgacg gcgagaacct caaggacacc gatcccaagg ccaagtcgat ccaggagtac
     8761 cgcgatgccg ccggcgtcga cgagggcatg aacggcctgt cgacccgttt cgccttcaag
     8821 atcctctcca aggtgttcaa cttcgacccc cacgaggtgg cggccaaccc ggtgcacctg
     8881 ctctacgtgc tcgagcagca gatcgagcag gagcagttcc cggcggaaac ccgcgagcgc
     8941 tacctgcgct tcctcaagga atatctggcg ccgcgctacg tcgagttcat cggcaaggaa
     9001 atccagaccg cctacctgga gtcctacagc gagtacgggc agaacatctt cgaccgctac
     9061 gtgctgtacg ccgacttctg gatccaggac caggagtacc gcgac
//
LOCUS       DATCUN010000141         1998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_43781, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000141 DATCUN010000000
VERSION     DATCUN010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43781"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VIO83_05810"
     CDS             <1..699
                     /locus_tag="VIO83_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009868047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8330824.1"
                     /translation="HPGSRAECELRAVDDLFHFNDPDGCRVEVAVGLQDAATPFVPGR
                     PLGGFRTGELGLGHVAFITPKFEEMCHLYKEVLQFRLSDYTSVPFRVEFLHVNPRHHT
                     LGIAYTGGPRKLYHLMIEYNDFDDLGRAHDIALENPDSIGVTFGRHINDHMTSFYLKN
                     PDGWMLELGWAGRTIGPDWQVEELSGMSLWGHDRTWLPADKRQLARDILKDLSKRGLR
                     APVVTTSQTAKVSQ"
     gene            696..1553
                     /locus_tag="VIO83_05815"
     CDS             696..1553
                     /locus_tag="VIO83_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8330825.1"
                     /translation="MTTLTPAASPEVANSLQVGDCTINYHDQGQGEAILLIHGSGPGV
                     TAWANWRGVIPALAERARVIAPDMLGFGYTRCPMDRPLDPDAWVAQLVGLLDALDIPK
                     VSVVGNSFGGAIALAFALRHPQRVSRLVLMGAAGLSFPLTAGLDKVWGYQPSLEAMRE
                     LMQVFAYDHSIITDDLVRMRFEASIRDDVQSRFAQLFPAPRQQGIEMLAVDEQALRQL
                     PQPTLIIHGRDDQVIPLEASERLLRLIPHAQLHVFGECGHWVQIERAAAFSRLLIDFL
                     CTERNEGAL"
     gene            1550..1987
                     /locus_tag="VIO83_05820"
     CDS             1550..1987
                     /locus_tag="VIO83_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEY8330826.1"
                     /translation="MNKDTIRGRWNILSWEQVYDDGRVVHPMGTELEGFIEYSPYGMF
                     CAIAKKDRAPFTSGGQWSASDAEKAAAYGSYLTYAGAWDVEGNVVQHHVRHSLFPNWE
                     GGSQRRLAQLEDGVLSLTARLEEGTPEARTARLVWTRALPAVG"
BASE COUNT          324 a          725 c          638 g          311 t
ORIGIN      
        1 cacccgggca gccgcgcgga gtgcgagctg cgcgcggtcg acgacctgtt ccacttcaat
       61 gatccggacg gctgccgcgt cgaggtcgcc gtcggcctgc aggacgccgc cacgcccttc
      121 gtccccggcc ggccgctggg cggcttccgc accggcgagc tgggcctcgg ccacgtcgcc
      181 ttcatcaccc cgaagttcga ggagatgtgc cacctgtaca aggaggtgct gcagttccgc
      241 ctcagcgact acaccagcgt gccgttccgc gtcgagttcc tccacgtcaa cccgcgccac
      301 cacaccctgg gcatcgccta caccggcggg ccgcgcaagc tctatcacct gatgatcgag
      361 tacaacgact tcgacgatct cggccgcgcc cacgacatcg cgctggagaa tcccgactcg
      421 atcggcgtca ccttcggccg ccacatcaac gaccacatga cctcgttcta cctgaagaac
      481 ccggacggct ggatgctcga gctgggctgg gcgggccgca ccatcggccc cgactggcag
      541 gtcgaggaac tgtccgggat gagcctgtgg ggtcacgacc gcacctggct gccggccgac
      601 aagcgccagc tggcccggga cattctcaag gacctctcca agcgcggcct gcgcgccccc
      661 gtagtaacca ccagccaaac cgccaaggtg agccaatgac gacccttacc cccgccgcct
      721 ccccggaggt ggccaacagc ctccaggtcg gcgactgcac gatcaactac cacgaccagg
      781 gccagggcga agccatcctg ctgatccacg gctcgggccc cggtgtcacc gcctgggcca
      841 actggcgcgg ggtgatcccg gcgctcgccg aacgggcgcg ggtgatcgcc cccgacatgc
      901 tgggcttcgg ctacacccgc tgcccgatgg accgcccgct cgatccggac gcctgggtgg
      961 cgcagttggt cggcctgctc gacgccctgg acatccccaa ggtgtcggtg gtcggcaact
     1021 ccttcggcgg tgccatcgcc ctggccttcg ccctgcgcca tccgcagcgg gtcagccgcc
     1081 tggtgctgat gggcgccgcc ggcctgtcgt tcccgctcac cgcgggcctc gacaaggtgt
     1141 ggggctacca gccgtcgctg gaggcgatgc gcgagctgat gcaggtgttc gcctacgacc
     1201 acagcatcat caccgacgac ctggtgcgca tgcgctttga ggcgagcatc cgcgacgacg
     1261 tgcagagccg cttcgcccag ctgttcccgg cgccgcgcca gcagggcatc gagatgctcg
     1321 cggtggacga gcaggcgctg cgccagttgc cgcagccgac cctgatcatc cacggtcgcg
     1381 acgaccaggt gatcccgctg gaggcttccg agcggttgct ccgcctgatc ccccatgccc
     1441 agctgcacgt gttcggcgaa tgcggccact gggtacagat cgagcgggct gcggcattca
     1501 gccgcctgct catcgatttt ctatgcactg agcgaaacga gggcgcgcta tgaacaaaga
     1561 caccatccgc ggacgctgga acattctttc ctgggaacag gtttacgacg acggccgtgt
     1621 cgtccatccg atgggcacgg agctggaagg cttcatcgaa tacagcccct acggcatgtt
     1681 ctgcgcgatc gccaagaagg accgggcgcc cttcaccagc ggtgggcagt ggtcggccag
     1741 cgatgccgaa aaggccgccg cctatggcag ctacctgacc tatgcggggg cctgggatgt
     1801 cgagggcaac gtggtccagc accacgtgcg tcacagcctg ttccccaact gggaaggcgg
     1861 ctcccagcgg cgcctggcgc aactggagga tggcgtgctg tccctcaccg cccgcctcga
     1921 ggaaggcacc cccgaggccc gtaccgccag actggtgtgg acgcgagcct tgcccgccgt
     1981 gggttgaggt tccccggc
//
LOCUS       DATCUN010000142         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_156214, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000142 DATCUN010000000
VERSION     DATCUN010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156214"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VIO83_05825"
     CDS             complement(<1..264)
                     /locus_tag="VIO83_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4526/YPO1902 family pathogenicity island
                     replication protein"
                     /protein_id="HEY8330827.1"
                     /translation="MSAPHPLNQAVIAQALHDLRNGQLRRCKAMGFGEEELDALKHPE
                     LVSMLVNATVSWCSVSVNREVLKRLLSQVHDVEREIATVDRMLR"
     gene            complement(282..1352)
                     /locus_tag="VIO83_05830"
                     /pseudo
     CDS             complement(282..1352)
                     /locus_tag="VIO83_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1400..>1777
                     /locus_tag="VIO83_05835"
     CDS             1400..>1777
                     /locus_tag="VIO83_05835"
                     /inference="COORDINATES: protein motif:HMM:NF017892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HEY8330828.1"
                     /translation="MTAFGSNVSSDFFTAKSTKEDSPANRIAASGKAVQCENTGNVLW
                     CAMMKSGLQSQILFGSDENAEFIMSMVGSYNVSLTNDASGGKNFAAHPMPKLITGEGL
                     KILVEGSANLDVKVYQCDQDDPDC"
BASE COUNT          312 a          606 c          572 g          287 t
ORIGIN      
        1 gcgcagcatg cggtccaccg tggcgatctc ccgctccacg tcgtgcacct ggctcagcag
       61 ccgcttcaac acttcccggt tcaccgacac cgaacaccac gacaccgtgg cattcaccag
      121 catgctcacg agttcggggt gcttcagcgc atccagctcc tcctcgccga agcccatggc
      181 cttgcagcgg cgcaactggc cgttgcgcag gtcatgcagg gcctgggcga tcacggcctg
      241 attgagcggg tgcggtgccg acatacggga gcctcctgcg ctcaggaatc ctggctcgct
      301 acgccggctt ccagatccag caaccggcgc gccaggcgca gcagacggaa cagcttcacc
      361 agcgcagcat cgctcaggcg tgtggccgag ccgccgtggc catgcagcag cgccgccagc
      421 tcgtcggcca gccgcgcgcg gtccaatccg ttcgcggctg gtggcgccac gctcagtaca
      481 tgcagcaggg tcagcaccgc ccgcgcgaac gcaggcagcg ccttcgcctg gcccacggcc
      541 ggcgacacgc agacgaagcc gatgccgccg gcgctggcct cgatgtggtc ggcaaccgcc
      601 gcttcctcgc cgatttcgcg cgcgaactgc gcgatgtgca cgcgcaggcg atccggcgcg
      661 tccagtcccg gctcgatgta ccagacatcc gagatgggaa agagcccgcc agcctgtacg
      721 gggatcgccc gcagcgcatc ggcctcgaag tcgggcagct tgtcgccgag ctggtccgcg
      781 accatccgct ggatggactg caggcgctcg gtggtcggtg ccggtgtcac gatgtgccct
      841 tgcaggcgct cgtcgcgttg cccgtgctgg tcctccggcg cttcgggcga tgcaggcggt
      901 gtcgctgctg acgccgaagg cgcgaccggc gtggtgtcgc gcggcgcaga cgaggcgggt
      961 ggctgctgag gcgcggagac cggcgtggga ggaccagcag gcacgacagg cgatgccggt
     1021 ggcgtcggtg ccgccggttc gctggtcagc gcacgctggc ggctttcgct gtcgttgatc
     1081 tccagcgcca gcgtgtcgta gtccgcctcc agcagctcgg ccatctggcc gaccagctcg
     1141 tcctgcaccc gctgcggcga gaagtcgtcc ggctgtgcgt cgaactgcgt cagcacgtcc
     1201 tgaaacaagg tggcgaagtc cacggtcacg gtgcggccca gcacacgccg ctcccaggtg
     1261 cgctcgcacg ccttgcgcag caccgcgagc cggtccacct gatgccggcc caatccgccg
     1321 tacaacagcg tcgggatcgc cggcagcaga tacgaatgcc aaaaaggcat acgaagaaag
     1381 caccgacttc aaatccgcga tgacggcctt cgggtcgaac gtctcatctg acttctttac
     1441 cgcgaaatcc accaaagaag actcaccagc caaccggatt gctgcctctg gtaaggccgt
     1501 tcaatgtgag aacacaggga atgttctgtg gtgcgccatg atgaagtctg gcctccagtc
     1561 gcagatccta ttcggcagcg acgaaaacgc ggaattcatc atgtcgatgg tcggcagcta
     1621 caacgtgtcg ctcaccaacg acgccagcgg ggggaaaaac ttcgctgcgc atcccatgcc
     1681 gaagctgatc accggcgaag gtctgaagat tcttgttgag ggctcggcca accttgatgt
     1741 caaggtctac cagtgcgacc aggatgaccc ggactgc
//
LOCUS       DATCUN010000143         6198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_62019, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000143 DATCUN010000000
VERSION     DATCUN010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62019"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VIO83_05840"
     CDS             complement(<1..441)
                     /locus_tag="VIO83_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330829.1"
                     /translation="MNKAWWVPLALLLLAGCATPVQRGSIPVVEAGKPLGTGAVARAP
                     ASAPRVKPPAQTQTLPQDSGVVVMVPGAAAGAPIQTFPAGGVASAPISVGSGLGSTGS
                     VTAPAPMPAPMAGSGAVTLAADERLDGPVLALLTSAQQMERSGNL"
     gene            complement(535..2880)
                     /gene="mrcB"
                     /locus_tag="VIO83_05845"
     CDS             complement(535..2880)
                     /gene="mrcB"
                     /locus_tag="VIO83_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HEY8330830.1"
                     /translation="MTRSRTPSSRTPRSRKPAPRPARPWLGWLLKLGVVGLVLLAGLA
                     IYLDAVVQEKFSGKRWTVPAKVYARPLELFSGLKLAREDFITELQALGYRRTQKVQGP
                     GELAVNGNQVELHTRGFQFYEGTEPAQQLRVRFSGNQVAGLALANGGALAVARLEPLL
                     VGGIYPAHNEDRILIQLDQVPPYLLETLIAVEDREFYNHLGVSPKSIARAMWVNTTSG
                     ALVQGGSTLTQQLVKNFFLSNERSLTRKGTEALMAVLLELHYDKQQILEAYLNEVFLG
                     QDGQRAIHGFGLAGQYFFGQPLAELSLPQVALLVGMVKGPSYYNPRRYPERALERRNL
                     VLDLLAEQGVVSGEEVARAKKAPLGVSSRGSLANTSYPAFLDMVKRQLRQDYQDEDLT
                     EEGLRIFTSLDPILQLKAETAMAETLKRLGGRKGIDEVETAMVVTNPQTGEVQALLGS
                     RQPRFAGFNRALDAQRPIGSLVKPAVYLTALEKPSQYTLTSLLQDEPFSVKTADGQVW
                     QPKNYDHRAHGTIFLYQGLAQSYNLSTAKLGLELGVPSVLKTVARLGVEPDWPAYPSM
                     LLGAGALSPVQVATMYQTLANGGFSTPLRGIRSVLNAAGEPLKGYPYEVQQRFDPGAI
                     HLVQEAMRRVMREGTARSVYNQLPAALNVAGKTGTSNDSRDSWFAGFGQDLLAVVWMG
                     RDDNGKTPLTGATGALQVWASFMRTAAPTSLDATPPDNVTRAWVDPYSGQGSDASCPG
                     ALQMPYIRGSEPPAGAPCGLPQELQAPAEQVMDWVRGWLQQ"
     gene            2995..4536
                     /locus_tag="VIO83_05850"
     CDS             2995..4536
                     /locus_tag="VIO83_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8330831.1"
                     /translation="MTQSLIEALQNPALYPHPVEGFEVIETHISWVLLTGPYAYKLKK
                     PVNFGFLDFSSREAREHFCREELRLNQRTAPDVYLEVLPVTGSLEAPQLGGDGPVLDH
                     VLKMRQFPQSGLLSNVLARGELTSAHIDELAREIADLHADAKHVPVEHALGSAEAVMA
                     PVRQNFEQIRPMLEDAADLAQLQHLEDWAEDTFTRLEPLLDRRKVDGFIRECHGDIHL
                     ANVTLLDDHVLLFDCIEFNEPFRLIDVISDAAFLAMDLEDRGLKALSRRFINAYLELT
                     GDYQGLTLLPFYKAYRAMVRAKISLFRLAQEQDPAERAAILRQYRNYAALAESYSAIP
                     SRFLAITHGVSAVGKSHVAMRLVEAFGAIRLRSDVERKRLFPAAAEAELYSAAATLAT
                     YSHLHQQAASVLLAGFPAVLDATYLKQAQREAAHAVAEQSGVPFLILDCHAPEAVIAT
                     WLAERQTAGVDPSDATLAVIRAQEAAREPLSAAEQGVSGRVDTHEAASLDGLVERIRR
                     RLPGL"
     gene            4692..4964
                     /locus_tag="VIO83_05855"
     CDS             4692..4964
                     /locus_tag="VIO83_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY8330832.1"
                     /translation="MNDELLEMKNLGKTSVLWLHAVGIHSASELRRRGSVAAYRAVLA
                     RGFNASRVLLYSIEGALLDRHWSELPREHKSRLLDQLATLDPRNKS"
     gene            5070..5864
                     /locus_tag="VIO83_05860"
     CDS             5070..5864
                     /locus_tag="VIO83_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8330833.1"
                     /translation="MYLTGEQPAYADQLINHLQSLPGQLLEGLAPSGPTLEIADLQDV
                     CAQLPGNQLYFIESGLLQARVDERPVFFLCEGDLLGLRQGLDLPDCQYSCEDPVRLRP
                     YSRNALFSHIHADIQRQELFTQYLIGQIGLLSDALARLRQPELRPSTGFQHFAAGEEL
                     IHQGDEADNVFIIVEGHAEAFVDGHRVGDVQKDEIFGAMAVFTGELRSATVIASEPCT
                     VMVIPKEQFISLMHSNPRIAHSLIESLARRIRLLNQEVTQLRTRDA"
     gene            complement(5942..>6198)
                     /locus_tag="VIO83_05865"
     CDS             complement(5942..>6198)
                     /locus_tag="VIO83_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron(III) ABC transporter"
                     /protein_id="HEY8330834.1"
                     /translation="DRRMAERLLAAPAPAVLLAGGFHVRRDLGVPLHLADLGAAEGVQ
                     VLQLAEVGAAVSAAQADFVWYTPGQPAQDHCAALRQRRSP"
BASE COUNT         1025 a         2265 c         1964 g          944 t
ORIGIN      
        1 aagattgccg ctgcgctcca tctgctgcgc cgaggtcagc agcgccagca ccggaccgtc
       61 aagccgctcg tcggcggcca gggtgaccgc gccggaaccg gccatcggcg caggcatggg
      121 cgccggcgcg gtaacggatc ccgtgctgcc cagaccgctg cccacgctga tcggcgcact
      181 cgccaccccg ccagccggga acgtctggat cggcgctccg gccgccgcgc cgggcaccat
      241 gaccaccacg ccggagtcct gcggcagcgt ttgcgtctgc gctggcggct tcacccgcgg
      301 cgcactggcc ggcgcacgcg cgaccgcccc ggtgcccagc ggcttgcccg cctccaccac
      361 cggaatcgac ccacgctgaa cgggagtcgc acagcccgcc agcagcaaca acgccagcgg
      421 cacccaccaa gccttgttca catccaatcc ccttgccatc tgtttatgcg caacggcttt
      481 cccagccgcc atcccttctg ccggttggac aaccgatcca gtcccggagt gccatcactg
      541 ctgcaaccaa ccgcgcaccc agtccatgac ctgctcggcc ggcgcctgca gctcctgcgg
      601 caggccgcag ggcgcgccgg ccggcggttc gctgccacga atatacggca tctgcagcgc
      661 acccgggcag ctcgcatcgg agccctggcc actataggga tcgacccagg cacgggtcac
      721 gttgtccggc ggcgtggcat ccagcgacgt cggcgcggcg gtgcgcatga agctggccca
      781 cacctgcagc gcgccggtgg caccggtcag cggcgtcttg ccgttgtcgt cgcggcccat
      841 ccacaccacg gccagcaggt cctggccgaa gccggcgaac cagctgtcgc gcgagtcgtt
      901 gctggtaccg gtcttgccgg cgacgttcag cgcagcaggc agctggttgt acaccgagcg
      961 cgccgtcccc tcgcgcatca cccggcgcat cgcttcctgc accagatgga tggcgcccgg
     1021 atcgaagcgc tgctgcacct cgtagggata gcccttgagc ggctcgccgg ctgcattcag
     1081 cacactgcgg atgccgcgca gcggggtgct gaagccgccg ttggccagcg tctgatacat
     1141 ggtcgccacc tgcaccggac tgagcgcgcc ggcacccagc aacatcgacg gataggccgg
     1201 ccagtccggc tcgacgccca ggcgcgcgac ggtcttcagc acgctgggca cgcccagctc
     1261 gagccccagc ttggcggtcg acaggttgta ggactgcgcc agcccctgat agagaaaaat
     1321 ggtgccgtgg gcccggtggt cgtagttctt cggctgccag acctggccgt cggcggtctt
     1381 gaccgagaac ggctcgtcct gaaggagact ggtcaaggtg tactgactcg gcttttccag
     1441 cgcggtcagg tagaccgccg gcttgaccag cgagccgatc ggccgctgcg catccagcgc
     1501 ccggttgaag ccggcgaagc gcggctggcg actgcccagc agcgcctgca cctcgccggt
     1561 ctgcggattg gtcaccacca tcgccgtctc gacctcgtcg atacccttgc gcccgcccag
     1621 gcgcttgagg gtttcggcca tcgccgtttc ggccttgagc tgcaggatcg gatcgagact
     1681 ggtgaagatg cgcagcccct cctcggtcag gtcctcgtcc tggtagtcct ggcgcaactg
     1741 gcgcttgacc atgtcgagga aggccggata ggaggtattg gccagactgc cgcgactgct
     1801 gactcccagc ggggcctttt tcgcccgtgc cacttcttcg ccgctgacca cgccctgctc
     1861 ggccagcaga tcgaggacca ggttgcgccg ctccagcgcc cgctccggat agcgccgcgg
     1921 gttgtagtag gacggcccct tgaccatgcc gaccagcagc gccacctgcg gcaggctcag
     1981 ctcggccagc ggctgaccga agaagtactg gccggccaga ccgaagccgt gaatcgcgcg
     2041 ctggccgtcc tgaccgagaa acacctcgtt gaggtaggcc tcgaggatct gctgcttgtc
     2101 gtaatgcagc tccagcaata ccgccatcag cgcctcggtg cccttgcggg tcaggctgcg
     2161 ctcgttgctg aggaagaagt tcttcaccag ctgctgggtc agggtactgc cgccctgcac
     2221 cagcgcaccg gaggtggtgt tgacccacat ggcgcgggcg atcgacttgg gcgatacgcc
     2281 gagatggttg tagaactcgc gatcctcgac cgcgatcagc gtttccagca gatacggcgg
     2341 cacctggtcc agctggatca ggatgcggtc ctcgttgtgc gccggataaa tgccgccgac
     2401 cagcagcggc tccagccgcg ccaccgccag cgcgccgcca ttggccagcg cgagcccggc
     2461 cacctgattg ccggaaaagc gcacgcgcag ttgctgcgcc ggctcggtgc cttcgtagaa
     2521 ctggaaaccg cgggtatgca gttcgacctg attgccgttc accgccagct cgccaggccc
     2581 ctggaccttc tgggtgcgcc ggtagccaag ggcctgcagt tcggtgatga agtcctcgcg
     2641 cgccagcttg agcccggaga acagctccag cggccgggca tagaccttgg ccggcacggt
     2701 ccagcgcttg ccggagaact tctcctggac cacggcgtcg aggtagatcg ccaggccggc
     2761 cagcagcacc aggccgacca caccgagttt gagcagccag cccagccacg gtcgggccgg
     2821 gcgcggcgcc ggtttgcgag aacgaggggt acgcgaagag ggggtacgag atcttgtcat
     2881 gttgggcatt atacgtggtt ggacccgggc ggatgacacg cccggcggtt tgcacgacgg
     2941 aacgcagtcg ccataatgga cgattagaca tgttcccgac gaaaaggatc gcctgtgacc
     3001 cagtccctga tcgaagccct gcagaacccc gccctgtacc ctcacccggt ggagggcttc
     3061 gaagtcatcg agacgcacat ctcctgggtt ctgctcaccg gcccctatgc ctacaagctg
     3121 aagaagccgg tgaatttcgg cttcctcgac ttcagcagcc gcgaggcgcg cgagcacttc
     3181 tgccgcgagg agttgcgcct caaccagcgc accgccccgg acgtctacct ggaggtcctg
     3241 ccggtcaccg gcagcctcga agccccgcag ctcggtggcg acggcccggt cctcgatcat
     3301 gtcctgaaga tgcgccagtt cccgcagagc ggcctgctgt ccaacgtgct ggcccgcggc
     3361 gaactgacca gcgcgcacat cgacgagctg gcccgtgaga ttgccgacct gcacgccgac
     3421 gccaagcacg tgccggtgga acatgcgctg ggcagcgccg aagcggtgat ggcgccggta
     3481 cggcagaact tcgagcagat ccgcccgatg ctcgaggacg ccgccgacct ggcccagttg
     3541 cagcacctgg aggactgggc cgaggacacc ttcacccgcc tcgagccgct gctcgaccgg
     3601 cgcaaggtcg acggcttcat ccgcgagtgc cacggcgaca tccacctggc caacgtcacc
     3661 ctgctcgacg accacgtgct gctgttcgac tgcatcgagt tcaacgagcc gttccgcctg
     3721 atcgacgtga tcagcgacgc cgccttcctc gccatggacc tcgaggaccg cggtctcaag
     3781 gcgctgtcgc gacgtttcat caatgcctat ctggagctga ccggcgacta ccagggcctg
     3841 accctgctgc cgttctacaa ggcttaccgc gccatggtcc gcgccaagat cagcctgttc
     3901 cgcctggccc aagagcagga tccggccgag cgtgcggcca tcctgcgcca ataccgcaac
     3961 tatgccgccc tggcggagag ctacagcgcc attccctcgc gttttctggc catcacccac
     4021 ggcgtgtccg cggtcggcaa gagccacgtg gccatgcgtc tggtggaggc cttcggcgcc
     4081 atccgcctgc gctcggacgt cgagcgcaag cgcctgttcc cggcggcggc cgaggccgag
     4141 ttgtattccg ccgcggccac cctcgccacc tacagccacc tgcaccagca ggccgccagc
     4201 gtcctgctgg ccggcttccc ggcagtgctg gacgctacct acctcaagca ggcccagcgc
     4261 gaggccgccc acgccgtggc cgagcagagc ggcgtaccct tcctgatcct cgactgccac
     4321 gctccggagg cggtcatcgc cacctggctg gccgagcgcc agacggccgg cgtggaccct
     4381 tccgacgcca ccctcgccgt gatccgcgcc caggaagccg cccgcgaacc gctgtccgcg
     4441 gccgaacaag gcgtcagcgg gcgggtcgac acccacgagg ccgctagcct cgacggtctg
     4501 gtcgagcgca tccggcgccg cctgccggga ctctgaagct cgcccgccgc ggccgccccg
     4561 ggccgcgacc ccgcgactga cggcactcgc aggctcgcca gcgccaacta gacttgccgg
     4621 cgagaggcca gcaaagagcg tggcagcgca gtacgccaag cgggtgcaaa tccggggcaa
     4681 ggggagtggg catgaacgac gagttgctgg agatgaagaa cctcggcaag acgtcggtgc
     4741 tgtggttgca tgccgtcggc atccacagcg ccagcgagct gcgccgccgc ggttcggtcg
     4801 ccgcctaccg ggcggtactc gcccgcggct tcaatgcctc gcgggtgttg ctctactcga
     4861 tcgaaggcgc cctgctcgac cggcactgga gcgaactgcc gcgcgaacac aagtcgcgcc
     4921 tgctggacca gctcgccacc ctcgacccgc gcaacaagag ctagctcaca acctccgcag
     4981 cacccaattt acctggcggg gctaaaaccc tattgtctgc aggcgcttgg cgcccacgtt
     5041 cgatctgccc cccccaagga atcgcgccca tgtacctcac cggcgagcaa ccggcctatg
     5101 ccgaccaact gatcaaccac ctgcagagcc tgcccgggca actgctggaa ggcctcgcgc
     5161 cctcggggcc gacgctggag atcgccgacc tgcaggatgt ctgcgcacaa ctgcccggca
     5221 accagctgta cttcatcgaa agcggcctgc tgcaagcccg cgttgacgag cggccggtat
     5281 tcttcctctg cgaaggcgac ctgctcggcc tgcgccaggg cctcgacctg ccggactgcc
     5341 agtacagctg cgaagatccg gtacgcctgc gcccctactc gcgcaacgcc ctgttcagcc
     5401 acatccatgc cgacatccag cgccaggagc tgttcaccca gtacctgatc ggccagatcg
     5461 gcctgctctc cgacgccctc gcccgcctgc gccagccaga attgcgcccg agcaccggct
     5521 tccagcactt cgccgccggc gaggagctga tccaccaggg cgacgaggcg gacaacgtgt
     5581 tcatcatcgt cgagggccat gccgaagcct tcgtcgacgg ccaccgcgtc ggcgacgtgc
     5641 agaaggacga gatcttcggt gccatggcgg tcttcaccgg cgaactgcgc agcgccacgg
     5701 tgatcgccag cgagccgtgc acggtgatgg tgatccccaa ggagcagttc atcagcctga
     5761 tgcacagcaa cccgcgcatc gcccacagcc tgatcgaaag cctggcccgg cgcatccgcc
     5821 tgctcaacca ggaagtcacc cagctgcgca cccgcgacgc ctgaccggca tcagcacgta
     5881 ggcgaaaggc ggccggagtc gatccgcgcc gcctttttgc tgcctgccgt ttgcgcgccg
     5941 gtcacggcga gcgccgctgg cgcagcgccg cgcaatgatc ctgagctggc tgccccgggg
     6001 tgtaccagac gaaatcggcc tgcgccgcgc tgacggctgc gcccacctcc gccagctgca
     6061 gcacctgcac gccttctgcc gcgcccaggt cggccaggtg cagcgggacg ccgagatcgc
     6121 ggcggacatg gaagccgccg gccagcagca ccgccggtgc cggcgccgcc agcaggcgct
     6181 cggccatgcg ccggtcac
//
LOCUS       DATCUN010000144         5573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_118767, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000144 DATCUN010000000
VERSION     DATCUN010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118767"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VIO83_05870"
     CDS             complement(<1..438)
                     /locus_tag="VIO83_05870"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY8330835.1"
                     /translation="MSPWRLLLTRPAEDCAALAQTLAERGIFGHCLPLLEIEALTETP
                     EQRTLFLELDRYSAVVVVSKPAARLGLELLDRYWPLPPARQHWFAVGAATGALLADYG
                     LDASWPSAGDDSEALLALPELQQAVDLPNPRVLILRGEGGRDWL"
     gene            complement(435..1373)
                     /gene="hemC"
                     /locus_tag="VIO83_05875"
     CDS             complement(435..1373)
                     /gene="hemC"
                     /locus_tag="VIO83_05875"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY8330836.1"
                     /translation="MSREIRIATRKSALALWQAEHVKARLEQAHPGLKVSLVPMVSRG
                     DKLLDAPLAKIGGKGLFVKELETALLENEADIAVHSMKDVPMDFPAGLGLYCICERED
                     PRDAFVSNTFASLDELPAGSVVGTSSLRRQAQLLARRPDLKIQFLRGNVNTRLAKLDA
                     GEYDAIILAAAGLIRLGFEARIRSSISAEDSLPAGGQGAVGIECRSADAEVQALLAPL
                     HHLDTADRVNAERALNKHLNGGCQVPIACYALLEGDDLWLRGLVGQPDGGLLLRAEAR
                     APRSQAEALGVQVAEALLAQGADRILAAVYGESEQP"
     gene            complement(1479..2225)
                     /locus_tag="VIO83_05880"
     CDS             complement(1479..2225)
                     /locus_tag="VIO83_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY8330837.1"
                     /translation="MNVLIVDDEPLARERLARLLGQLDGYRVLEPSASNGEEALTLID
                     NLRPDIVLLDIRMPGLDGLQVAAKLCEREAPPAVIFCTAHDEFALEAFQVSAVGYLVK
                     PVRPEALEEALKKAERPNRMQLAALTRPAADGHGAPRSHISARTRKGIELIPLDQVIY
                     FIADHKYVTLRHEHGEVLLDEPLKALEDEFGERFVRIHRNALVARERIERLQRTGLGH
                     FQLYLRGLGDEALIVSRRHVAGVRKLMSEL"
     gene            complement(2222..3301)
                     /locus_tag="VIO83_05885"
     CDS             complement(2222..3301)
                     /locus_tag="VIO83_05885"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY8330838.1"
                     /translation="MSIPRFAGTRPPASSDDFFLPELCAPEALLSLVLLAELLVLVLV
                     LAEPLQPSFDWVRLALTSLFVQWLVLLSAAALCRLRPWLMSLPQALAGVLCGALVVAL
                     TLACTWAAGYFGLAAPRPQEGLLNLYLRHGLIALIMSGLVLRYFYLQSQWRRQQQAEL
                     RARLEALQARIRPHFLFNSLNSIASLIASDPDKAELAVLDLSDLFRASLATPGSLVAW
                     RDELALGRRYLSIEQYRLGERLQLDWQVAGVPDDLPIPQLTLQPLLENAIIHGIQPRI
                     DGGRIEVQASYHDGLFQLCVSNPYDSEPAAVSRGTRQALGNIDARLVALFGPQATLSS
                     ERRDGRHFTCLRYPCARPMQEARAS"
     gene            3458..4852
                     /gene="argH"
                     /locus_tag="VIO83_05890"
     CDS             3458..4852
                     /gene="argH"
                     /locus_tag="VIO83_05890"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY8330839.1"
                     /translation="MSSEKTNQSWGGRFSEPVDAFVARFTASVDFDKRLYRHDIMGSI
                     AHATMLEQAGVLTGAERDAIIAGLQQIQAEIEAGQFDWRVDLEDVHMNIEARLTDRIG
                     ITGKKLHTGRSRNDQVATDIRLWLRDEIDVILAEITRLQQGLLAQAGQHADVIMPGFT
                     HLQTAQPVSFGHHLLAWFEMLSRDYERLVDCRKRVNRMPLGSAALAGTTYPIQREITC
                     QLLGFEAIGGNSLDGVSDRDFAIEFCAAASLAMMHLSRFSEELVLWTSAQFNFIELPD
                     RFCTGSSIMPQKKNPDVPELVRGKSGRVFGALMGLLTLMKGQPLAYNKDNQEDKEPLF
                     DAADTLRDSLRAFADMIPAIKPRVEVMREAARRGFSTATDLADYLVRKGLPFRDCHEI
                     VGHAVKYGVATGKDLAEMSLDELRQFSDQITDDVFAVLTLEGSVNARDHIGGTAPNQV
                     RAAVARGQALLAAR"
     gene            4976..5431
                     /locus_tag="VIO83_05895"
     CDS             4976..5431
                     /locus_tag="VIO83_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330840.1"
                     /translation="MSTADRPRFRVSADRQSASRRLRYVETSRPDDGSCTLCQLDEEN
                     LPAPDETPAMSHNSAEDDAVFAENKLIEAIENQIAAGEPAAAQATLNKLTLVGYPRED
                     AVRLMALILAHEIEGMLADNRPFDGAGYEQMLRALPELPEAVGDSEEDE"
     gene            5485..>5573
                     /locus_tag="VIO83_05900"
     CDS             5485..>5573
                     /locus_tag="VIO83_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5669 family protein"
                     /protein_id="HEY8330841.1"
                     /translation="MTLTPELIAELELLALFNPDNTLEGLKVH"
BASE COUNT          918 a         1974 c         1887 g          794 t
ORIGIN      
        1 gagccagtcg cgcccgccct caccgcgcag gatcagcacc cgcgggttgg gcaggtcgac
       61 ggcctgctgc agctcgggca gcgccagcag cgcctcgctg tcgtcgccgg cggacggcca
      121 gctggcatcc aggccgtagt ccgcgagcag cgcgccagtg gcggcgccga cggcaaacca
      181 gtgctggcgc gccggcggca acggccagta gcgatcgagc agttcgagac ccaggcgcgc
      241 cgccggcttg ctcaccacca ccaccgcgct gtagcggtcg agctcgagga acagcgtgcg
      301 ctgctccggg gtttcggtca gcgcctcgat ctccagcagc ggcaggcagt ggccgaagat
      361 gccccgctcc gccagggtct gcgccagcgc ggcgcagtcc tcggccggac gggtcagcag
      421 caggcgccag gggctcacgg ctgctcggac tcgccgtaga ccgcggcgag aatgcggtcg
      481 gcgccctgag ccagcagcgc ttcggccacc tgcacgccga gggcttcagc ctggctgcgc
      541 ggggcacggg cctcggcgcg cagcagcagg ccaccgtcgg gctggccgac caggccgcgc
      601 agccacaggt cgtcaccctc gagcagcgcg tagcaggcaa tcggcacctg gcagccgccg
      661 ttgaggtgct tgttcagcgc gcgctcggcg ttgacccggt cggcggtatc gaggtggtgc
      721 aaaggcgcca gcagggcctg cacctcggcg tcggcgctac ggcactcgat gcccaccgcg
      781 ccctggccgc cggccggcag gctgtcctcg gcgctgatcg aggagcgaat ccgcgcctcg
      841 aagccgaggc ggatcagccc ggcggcggcg aggatgatgg cgtcgtactc gccggcatcc
      901 agcttggcca ggcgggtgtt gacgttgccg cgcaggaact ggatcttcag gtcgggacgg
      961 cgtgccagca gctgggcctg acggcgcagg ctggaggtac ccaccacgct gccggccggc
     1021 agctcgtcga ggctggcgaa ggtgttggaa acgaaggcat cgcgcgggtc ttcgcgctcg
     1081 cagatgcagt acaggccgag tcctgccggg aagtccatcg gcacgtcctt catcgagtgc
     1141 acggcgatgt ccgcctcgtt ctccagcagg gcggtctcca gttccttgac gaacaggccc
     1201 ttgccgccga tcttcgccag cggcgcgtcg agcagcttgt cgccgcgact gaccatcggc
     1261 accaggctga ctttcagccc cggatgggcc tgttccaggc gggccttgac gtgttcggcc
     1321 tgccacaggg cgagggcact cttgcgggtg gcgatgcgga tttcgcggga catgggcaat
     1381 tccagagaca gaatggactg ccgcgatgat aacaggctcg gcgcgccggc tggattggcc
     1441 gggagcaagg aaaggccgcg cgggcgcggc cgcagggatc agagttcgct catcagcttg
     1501 cgcacgccgg ccacgtggcg gcgactgacg atcagcgcct cgtcgccgag accgcgcagg
     1561 tacagctgga aatggccgag gccggtgcgc tgcagccgct cgatgcgctc gcgggccacc
     1621 agcgcgttgc gatggatgcg cacgaagcgc tcgccgaact cgtcctccag cgccttcagc
     1681 ggctcgtcga gcagcacctc gccgtgctcg tggcgcaggg tcacgtactt gtggtcggcg
     1741 atgaagtaga tcacctggtc cagcgggatc agctcgatgc ctttgcgggt gcgcgcactg
     1801 atgtggctgc gcggtgcgcc gtggccgtcc gccgccggac gggtcagcgc cgccagctgc
     1861 atgcggttgg gccgctcggc cttcttcagc gcctcttcca gggcctcggg gcgcaccggc
     1921 ttgaccaggt agccgaccgc gctgacctgg aacgcctcga gggcgaattc gtcgtgggcg
     1981 gtgcagaaga tcaccgccgg cggcgcctcg cgctcgcaca gtttggcggc cacctgtagc
     2041 ccgtcgagac cgggcatgcg gatgtcgagc agcacgatgt ccggcctgag gttgtcgatc
     2101 agggtcagtg cctcttcgcc attgctggcg gaaggctcga gaacacggta gccgtccagt
     2161 tgcccgagca gtcgggccag gcgctcgcgc gccaggggtt cgtcgtcgac gatcaggaca
     2221 ttcatgatgc tctggcttcc tgcatgggtc tcgcacaagg atagcgtaga caggtgaagt
     2281 gccgtccgtc acggcgttca ctgctaagag tggcctgcgg cccgaaaagt gccacaagac
     2341 gggcgtcgat attgccgagc gcctgccgcg tgccgcgcga aaccgccgcc ggctcgctgt
     2401 cgtagggatt gctgacgcac agctggaaca ggccatcgtg gtagctggcc tgcacctcga
     2461 tccggccgcc gtcgatgcgc ggctggatgc cgtggatgat ggcgttctcc agcagcggct
     2521 gcagggtcag ctgcgggatc ggcaggtcgt ccggcacgcc ggcgacctgc cagtccagct
     2581 gcaggcgttc gcccaggcgg tactgctcga tcgacagata gcgccgcccc agcgccagct
     2641 cgtcgcgcca ggcgaccagg ctgccgggcg tggccagact ggcgcggaac aggtcggaga
     2701 ggtcgagcac cgccagctcg gccttgtccg ggtcgctggc gatcaggctg gcgatgctgt
     2761 tcaggctgtt gaacaggaaa tgcgggcgga tgcgcgcctg cagggcttcg agccgcgcgc
     2821 gcagctcggc ctgctgctgg cgccgccact ggctctgcag gtagaagtag cgcagcacca
     2881 ggccggacat gatcagcgcg atcaggccgt ggcgcaggta cagattgagc aggccctcct
     2941 gcggccgcgg cgcagccagg ccgaaatagc ccgccgccca ggtgcaggcc agggtcagcg
     3001 ccaccaccag cgcgccgcac agcacgccgg ccagcgcctg cggcaggctc atcagccagg
     3061 ggcgcaggcg gcacagcgcc gccgccgaaa gcagcaccag ccactgcacg aacagcgagg
     3121 tcagcgccag gcgcacccag tcgaacgacg gctgcagcgg ctcggccagc accagcacca
     3181 gcaccagcag ctcggccagc agcaccaggc tgagcagcgc ctccggcgca cacagctcgg
     3241 gcaggaagaa atcgtcggat gatgccggcg ggcgggtgcc ggcgaacctg ggtatggaca
     3301 tccctgcagt gtcgacccct accgttcgtc cggcaagcct gcagcggtgc ggggcttgac
     3361 ctgcgtcacc gaaatgccgc taccgggttt ttccgggcga actggtatca tgccggccag
     3421 cattccgtct tccgcacgca cagcacacga gataccgatg agcagcgaaa aaaccaacca
     3481 gtcctggggc ggccggttca gcgaaccggt cgacgccttc gtcgcccgct tcaccgcctc
     3541 cgtcgacttc gacaagcgcc tgtaccgcca cgacatcatg ggctccatcg cccacgccac
     3601 catgctggag caggccggcg tgctgaccgg cgccgagcgc gacgcgatca tcgccggcct
     3661 gcagcagatc caggccgaga tcgaggccgg ccagttcgac tggcgcgtcg acctggaaga
     3721 cgtgcacatg aacatcgagg cgcgcctgac cgaccgcatc ggcatcaccg gcaagaagct
     3781 gcacaccggg cgtagccgca acgaccaggt ggccaccgac atccgcctgt ggctgcgcga
     3841 cgagatcgac gtcatcctcg ccgagatcac ccgcctgcag cagggcctgc tggcccaggc
     3901 tgggcagcac gccgacgtca tcatgcccgg cttcacccac ctgcagaccg cccagccggt
     3961 gagcttcggc caccacctgc tggcctggtt cgagatgctg tcgcgcgact acgagcgcct
     4021 ggtcgactgc cgcaagcgcg tcaaccgcat gccgctcggc tcggcggcgc tggccggcac
     4081 cacctacccg atccagcgcg agatcacctg ccagctgctc ggcttcgagg ccatcggcgg
     4141 caactcgctg gacggcgtgt cggaccgcga cttcgccatc gaattctgcg ccgccgcctc
     4201 gctggcgatg atgcacctgt cgcgcttctc cgaagagctg gtgctgtgga cttccgcgca
     4261 gttcaacttc atcgaactgc cggatcgctt ctgcaccggc tcgtcgatca tgccgcagaa
     4321 gaagaacccc gacgtccccg agctggtgcg cggcaaatcg ggccgcgtgt tcggcgcgct
     4381 gatgggcctg ctgaccctga tgaagggcca gccgctggcc tacaacaagg acaaccagga
     4441 ggacaaggag ccgctgttcg acgccgccga caccctgcgc gactccctgc gcgccttcgc
     4501 cgacatgatc ccggcgatca agccgcgcgt cgaggtgatg cgcgaggccg cccgccgcgg
     4561 cttctccacc gccaccgacc tggccgacta cctggtgcgc aagggcctgc cgttccgcga
     4621 ctgccacgag atcgtcggcc acgcggtgaa gtacggcgtg gcgaccggca aggatctggc
     4681 cgagatgagc ctcgacgagc tgcgccagtt cagcgaccag atcaccgacg acgtgttcgc
     4741 cgtgctgacc ctggaaggct cggtcaacgc ccgcgaccat atcggcggca ccgcgcccaa
     4801 ccaggtgcgc gccgccgtcg cccgcggcca ggccctgctg gccgcacgct gatcccccgc
     4861 agggtgcgcc atgcgcaccc tgcgcgaccg ggatggccgc ccgcgaccaa cgagcgcttg
     4921 ccgcccccac catcggcggg ttatgaagga gccatcagct gctccggagc ccgccatgtc
     4981 caccgccgac cgcccgcgct ttcgcgtcag cgccgaccgc cagtcggcca gccggcgcct
     5041 gcgctacgtg gaaaccagcc gcccggatga tggcagctgc accctctgcc agctggacga
     5101 agaaaatctt ccggcgcctg acgagacccc tgccatgagt cacaactccg ccgaagacga
     5161 tgcggtcttc gccgaaaaca agctgatcga agccatcgag aaccagatcg ccgccggcga
     5221 gccggccgcg gcgcaggcta ccctcaacaa actgaccctg gtcggctacc cgcgcgagga
     5281 cgccgtgcgc ctgatggcgc tgatcctcgc ccacgagatc gaaggcatgc tggccgacaa
     5341 ccgtccgttc gacggcgccg gttacgagca gatgctgcgc gccctgccgg agctgccgga
     5401 agccgtgggc gacagcgaag aagacgaata acaaatatcc tgcgggtgcc gccggccccc
     5461 gccctgtcct gcactggagg caccatgacc ctgacccccg agctgatcgc cgaactggag
     5521 ctgctcgccc tgttcaaccc ggacaacacc ctggaaggcc tcaaggtcca tca
//
LOCUS       DATCUN010000145         4016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_80888, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000145 DATCUN010000000
VERSION     DATCUN010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80888"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /gene="kdsA"
                     /locus_tag="VIO83_05905"
     CDS             <1..649
                     /gene="kdsA"
                     /locus_tag="VIO83_05905"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014754147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY8330842.1"
                     /translation="YRGVGLEEGLRIFEEVKRRFGVPVITDVHEVAQAAPVAEVVDVL
                     QIPAFLARQTDLVAAIARTGRAVNIKKPQFLSPTQIKHIVSKIREAGNENIILCERGS
                     QFGYDNLVVDMLGFREMIESTGGLPAIFDVTHSLQRRDAGSEASGGRRRQVAELARSG
                     MALGLGGLFLEAHRDPDHARCDGPSALPLSALEPFLAQIKALDELVKGFPELDIR"
     gene            777..853
                     /locus_tag="VIO83_05910"
     tRNA            777..853
                     /locus_tag="VIO83_05910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:811..813,aa:Arg,seq:cct)
     gene            1186..1668
                     /locus_tag="VIO83_05915"
     CDS             1186..1668
                     /locus_tag="VIO83_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330843.1"
                     /translation="MLKGFKPLAAAALCLLGLSAFAQNAAAAQWVDSGRTAYLKFSFF
                     GSFAEHTCNAGTAPIQDYPGTCANDAASGWTANHSASSGLSNEQVNSCGVNPNQLGSA
                     CYGLAYAINGQAYAGGLPAACNVGARAVVTSTSMETVEIQYEELDVDFIELAREYVCQ
                     "
     gene            2263..2601
                     /locus_tag="VIO83_05920"
     CDS             2263..2601
                     /locus_tag="VIO83_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEY8330844.1"
                     /translation="MQAFNIGATIRLDFTPVTRWDEYHDAFVAAFGLPAFYGRNGHAL
                     LDCLSSLRDPDAGMSRVVVAPGEVVLVQVDGLAAMPRELRESFIMTLELLSERCAARE
                     QSSPIVLVII"
     gene            complement(2594..3718)
                     /locus_tag="VIO83_05925"
     CDS             complement(2594..3718)
                     /locus_tag="VIO83_05925"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEY8330845.1"
                     /translation="MAVSKWMSALPLCLLLAPGAHAAAPTDGVRQVPTFALPVSPYLS
                     PQAQDSVRSAVIHGDPTAKLDNAAVLAQLDSIRKGTDQWAYGEIAELRKRFAVELHHE
                     TWGGIPVVRVQPRSAPADPANQRLLIELHGGAFVFGRAESLGLLEAIPVAAMTGMTVL
                     AVEYRQGPEHRFPAASEDVEAVYLAALARLPAAHIGIFGCSAGGVLTGESLAWFAKEK
                     LPMPGAAGLFCAGGDARYGGDSRYVMAAINDALLPHADGTLPIMEDLYYGDVDFRDPL
                     VSPVFSEPLLAQFPPTLFITGTRAAELSNVSYMHSRLVDLERVSDLHVWEGLGHAFHL
                     DAKLPESQQAYRVMARFFAKHLGTALPASHPAPAAAAATR"
BASE COUNT          706 a         1309 c         1305 g          696 t
ORIGIN      
        1 ctaccgcggc gtcggtctgg aagaagggct gcgcatcttc gaggaggtca agcgtcgttt
       61 cggcgtgccg gtgatcaccg acgtgcacga agtggcccag gctgccccgg tggccgaggt
      121 tgtcgacgtg ctgcagattc ccgccttcct ggcccgtcag accgacctgg tggcggccat
      181 tgcccggacc ggccgcgcgg tcaatatcaa gaagccgcag ttcctcagcc ccacgcagat
      241 caagcacatc gtgtccaaga tccgcgaggc cggcaatgag aacatcatcc tgtgcgagcg
      301 cgggtcgcag ttcggctacg acaacctggt cgtggacatg ctgggcttcc gcgagatgat
      361 cgagtccacc ggcggcctgc cggcgatctt tgacgtgacc cacagcctgc agcgccgcga
      421 cgcgggcagc gaagcctcgg gtggccgccg ccgccaggtg gccgagctgg cccgttcggg
      481 catggcgctg ggcttgggtg ggctgttcct tgaggcgcac cgtgacccgg accacgcacg
      541 ctgcgatggc cccagtgcgc tgcctctgtc ggcactggag ccgttcctgg cccagatcaa
      601 ggcgctggac gaactggtca agggcttccc ggaactggat atccgctgag tcggcctgcc
      661 ggcattcggc ggcgacggcc ttggtcccgt cgctgaatgt gggtatccta catggctgca
      721 cgccgtcaaa aacgcgcgca gccagcggtc tggcgagtct tatccgcccc gcacttggcc
      781 ccgtagctca gctggataga gcgtccccct cctaagggga aggtcgcccg ttcgaatcgg
      841 gccggggtca ccatcaatca agtacttggc tgaattccaa cgacacacgc ttggtatatt
      901 gcgccgcgtt ttggaacgct ttgggcacag ttggcgtgcc ggattggcag gagccgagga
      961 gcgtggaaat gatcaaggcg tgtcagcacc tgccgaagcc gccaggctgt tggagtcagg
     1021 gcgtggctaa gggcgtggat cgcgcgtagg ggctggagaa actctcgctg ctcgtagttt
     1081 ctacgctcag actgccaagc tcgtcatatt agcgtgcgga aagtgtggat aaactccgcc
     1141 ggcacgcacg atgcgtgcac tacgccaatt ccgaggacga tagtcatgtt gaaaggattt
     1201 aaacccctcg ccgcagctgc gctgtgcctg ctcggcctgt ccgccttcgc ccaaaacgct
     1261 gccgccgcgc aatgggtcga tagcggccgc actgcgtacc tcaagtttag cttcttcggc
     1321 agcttcgccg agcacacctg caacgcaggc actgcgccga tccaagatta cccgggtacc
     1381 tgtgccaacg atgcggccag cggctggacg gccaaccaca gtgcctccag cgggctgtcc
     1441 aacgagcagg tcaactcctg cggggttaat ccgaaccagc ttggctcggc ctgctacggc
     1501 ctagcctatg cgatcaacgg gcaagcctac gccggcggac tgccggccgc gtgcaacgtc
     1561 ggcgcgcgcg cggtggtcac ttcgaccagt atggaaaccg tcgagatcca gtacgaagag
     1621 ctcgatgtgg actttatcga gttggctcgc gagtacgtct gccagtaaac cggtagcaga
     1681 acgccgccgc cgagcgcgac ggcgttctgc acccctgacc gcctgtacgg cgaaccagtt
     1741 acggccagcc gtcgaatctg agaacctgac ccgtatcctg cgcgcatgct cccgcctgac
     1801 tatcgatggc gataagcggc cagcgggccc gacggctgcc ggatgcgccg ctgtgtgaca
     1861 gaggctattc ccgcctccgc aaaaagtcgt caaacaccac actgctgatc gcctgtctcg
     1921 ctttgcagcc tgggcagcgc atcgcaatgc caccgggcac tgccagcagt gtcgattcgt
     1981 cagccgcagt gaagtggtgg ttgcacttga ggcaagcggc ggtcacggaa gtgacgcgcg
     2041 ctgcgcgatc gctcgatagc tcgcgatcgc cagcaacttc aatcagatgg aagacacctg
     2101 tggaggacat cgttggctga tccgtcgtgt tgctgaagga gtagacgcaa gtagaccagt
     2161 gcaggtgtac tgcgccggtg cagtcatggt caacgtcagg tgtagagcgg aactcgccgc
     2221 cactgctcta gactatcggc cttaatctcc aggacggagc acatgcaagc gttcaacatt
     2281 ggcgccacga tccgcctgga tttcaccccg gtcacacgct gggacgaata ccacgatgcc
     2341 ttcgttgcag ctttcggcct gccggcattc tatggccgca acggtcatgc gctgctcgac
     2401 tgcctgtcct ccctccgcga ccccgacgca gggatgagcc gggtggtggt agcgcccggg
     2461 gaggtcgttc tggtgcaggt ggatggcctg gccgctatgc cgcgcgagct gcgcgagtcc
     2521 ttcatcatga cgctggaact gctcagtgaa cgctgcgcgg cgcgcgaaca gtcgtcgccc
     2581 attgtcctcg tgatcatcta gtcgcagctg ccgctgcggg ggcagggtgg ctggcgggca
     2641 gcgccgttcc gaggtgcttt gcaaagaacc tcgccatcac ccggtacgcc tgctggcttt
     2701 ccggcagctt ggcatccagg tggaacgcgt gccccagccc ctcccacaca tgcaggtccg
     2761 acacgcgctc caggtccacc agccggctat gcatgtagga cacattgctc agctccgccg
     2821 cgcgggtgcc ggtgatgaac agcgtgggcg gaaactgcgc cagcagcggt tcggaaaaca
     2881 ccggcgacac cagcgggtcg cggaagtcga cgtcgccgta gtagaggtct tccatgatgg
     2941 gcaatgtgcc gtcggcatgg ggcagcaagg cgtcgttgat ggcggccatg acgtagcgcg
     3001 agtcgccgcc gtaacgcgca tcgccgcccg cgcagaacag gccggcagcg ccgggcatgg
     3061 gcagtttttc cttcgcgaac caggccagtg attcaccggt gagcacgcca ccggccgagc
     3121 agccgaagat gccgatatgc gcggcgggca accgcgccag tgccgccagg tacaccgcct
     3181 cgacatcctc gctggccgca gggaagcggt gctcggggcc ctgacgatac tccacggcga
     3241 gcacagtcat gccggtcatc gccgccaccg ggatcgcctc gagcaggccc aggctttcgg
     3301 cgcggccgaa tacaaaggcg ccgccatgca gttcgatgag caggcgttga tttgcagggt
     3361 cagccggcgc actgcgtggc tgcacgcgca ccaccggaat gccgccccag gtttcgtggt
     3421 gcagctccac cgcgaagcgt ttgcgcagtt cggcgatctc gccataggcc cattggtcgg
     3481 tccccttgcg gatactgtcc agctgcgcga ggacggcggc attgtccagc ttggccgtgg
     3541 gatcgccgtg gatcactgcg ctgcgtacgc tgtcctgcgc ctgcgggctg agatagggcg
     3601 acaccggcaa tgcgaaggtg ggcacctgac gcacgccgtc agtaggcgct gcggcgtgcg
     3661 cgccgggagc cagtagcagg cacaacggaa gcgccgacat ccatttcgaa acagccatgt
     3721 gcacggtcca cgttgcggga atgcggcaca ggatagcgcc cggcgcggcc cggtactaag
     3781 cgtcaccctg ccgatacgtg cacggtgccg tcgccggttg ttgacgcgct accggcgatg
     3841 ctgtgccatc cccacccgtt ggagctaccc gatgaacgca tcgtcgcatt cgccgcccgg
     3901 cgtgcccccc acgcccacgc cagggtcgcc tgttgcgccg cccgtggaag tgccggtgag
     3961 cccggaaccg ccggtgaagg agccgcctgc cgaagcaccg ccccaaggtg atccgc
//
LOCUS       DATCUN010000146         9210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_212693, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000146 DATCUN010000000
VERSION     DATCUN010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9210
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212693"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /gene="nudE"
                     /locus_tag="VIO83_05930"
     CDS             <1..491
                     /gene="nudE"
                     /locus_tag="VIO83_05930"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010168971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HEY8330846.1"
                     /translation="RFSNGVERTYERLVNRGLGYGAVMVVALEDAETVLLIEEYCAGI
                     DAYELSLPKGLIEPGEDVLAAANRELKEEAGFGARQLEHITGLSLSPGYMSQSIQVVL
                     ARDLYAERLPGDEPESIRVERFSLRDISRLAAADNFSEGRALAALYLVRDLLTQRGEY
                     QP"
     gene            500..1318
                     /gene="cysQ"
                     /locus_tag="VIO83_05935"
     CDS             500..1318
                     /gene="cysQ"
                     /locus_tag="VIO83_05935"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEY8330847.1"
                     /translation="MSHPLLPAVVELVRQAGAATLPYWRADVAVELKADASPVTAADL
                     AAHRLLAAGLAQLAPEIHLLSEEDCEIGLAERRRWARWWLVDPLDGTKEFIAGSEEFT
                     VNVALIEQGKVVFGVVGVPARGLCFYGGAGLGAWRMADGEAQAIGVRIAPAEAFTVVA
                     SRRHSSPAQERLLSGLGERFGDLALASVGSSLKFCLLAEGSADCYPRLVPTSQWDTAA
                     AQGVLEGAGGEVLDLHGQPLDYPARESFLNPHFLALPQAAEWREELLQLALALD"
     gene            complement(1435..1887)
                     /locus_tag="VIO83_05940"
     CDS             complement(1435..1887)
                     /locus_tag="VIO83_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEY8330848.1"
                     /translation="MNPDCRYLEELLHHDIPLTRTMELRVQRWENHELRLSAPLAPNV
                     NHTGTMFGGSLYSLSVLAGWGWLTLRLREAGIDDGHIVIKGGQIDYPLPVVAEAVAIC
                     AAPSEEDWKKFETMYRRRGRARLYLDIRILAPSGETAVHLHGEYVLHK"
     gene            complement(2100..3290)
                     /gene="metK"
                     /locus_tag="VIO83_05945"
     CDS             complement(2100..3290)
                     /gene="metK"
                     /locus_tag="VIO83_05945"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY8330849.1"
                     /translation="MSEYSLFTSESVSEGHPDKIADQISDAVLDAIIAEDKHARVACE
                     TLVKTGVAIVAGEITTSAWIDLEQLVRDVIIDIGYNSSDVGYDGATCGIVNIIGKQSP
                     DINQGVDRAKPEDQGAGDQGLMFGYASNETDVLMPAPICFSHRLVERQAEARKSGLLP
                     WLRPDAKSQVTCRYENGKVVGIDAVVLSTQHSPEISQADLHEAVMELIVKHTLPAELL
                     HKDTQFHINPTGQFIIGGPVGDCGLTGRKIIVDSYGGMARHGGGAFSGKDPSKVDRSA
                     AYAGRYVAKNIVAAGLAERCEIQVSYAIGVAQPTSISINTFGTNKVSEDTIIKLVREV
                     FDLRPYAITKMLDLLHPMYKATAAYGHFGRTPQEVTVGDDTFTTFTWEKTDKAEALRA
                     AAGL"
     gene            complement(3354..4355)
                     /locus_tag="VIO83_05950"
     CDS             complement(3354..4355)
                     /locus_tag="VIO83_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8330850.1"
                     /translation="MSTQPAFRPDPSDDLAALCKAGGDPLRLNVLRVLACDSFGVLEL
                     AQIFAMRQSGMSHHLKVLAQAGLVATRREGNAIFYRRALAASGQTGSALQAALLEEVD
                     ALQLPAEVQLRIAEVQAQRAAAARDFFARTLEKFRAQQDLIAGLPQYRDSLLTLLDAL
                     HLPASASALEVGPGDGAFLPELARRFARVTALDSSPAMLELARQRCSAEGLANVELKL
                     ADALNDEQAPADCVVLNMVLHHFAEPARAMAQLARLLTAGGSLLLTELCSHHQSWARD
                     ACGDLWLGFDQDELARWAEDAGLTAGESLYLGLRNGFQIQVRHFAKPEVATRTTLKH"
     gene            4558..6558
                     /gene="tkt"
                     /locus_tag="VIO83_05955"
     CDS             4558..6558
                     /gene="tkt"
                     /locus_tag="VIO83_05955"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY8330851.1"
                     /translation="MPSRRERANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPQWADRDRFVLSNGHGSMLIYSLLHLTGYDLSIDDLKQFRQLHSRTPGHPE
                     YGYTPGVETTTGPLGQGIANAVGFAIAEKVLGAQFNRPGHAIVDHFTYAFLGDGCMME
                     GISHEVCSLAGTLGLGKLVAFYDDNGISIDGEVEGWFTDDTPARFEAYGWQVIRSVDG
                     HDAAAIKAAVEAARGETARPTLICCKTTIGFGSPNKGGKEECHGAPLGNDEIAATRAA
                     LGWNHGPFEIPADIYAEWDAKAAGAANEAEWNARFAAYAAEFPQLAAEFQRRIAGELP
                     ADFAEQAAAYVAEIAAKGETIASRKASQNALNAFGPLLPEFLGGSADLAGSNLTLWKG
                     CQSLSHADASGNYLHYGVREFGMSAIMNGVALHGGFIPYGATFLIFMEYARNAVRMSA
                     LMKQRVLYVFTHDSIGLGEDGPTHQPIEQLASLRGTPNLDTWRPCDAVESAVAWKYAI
                     ERNDGPSALIFSRQNLPHQARDAQQLADVARGGYVLKDCAGEPELILIATGSEIGLAV
                     QAFDALTAQGRKVRVVSMPSTSVFDQQDAAYKQAVLPVQVGARIAIEAAHADFWYKYV
                     GLEGRIIGMTTFGESAPAAQLFEEFGFTLDNVLSTAEELLDV"
     gene            6696..7754
                     /gene="epd"
                     /locus_tag="VIO83_05960"
     CDS             6696..7754
                     /gene="epd"
                     /locus_tag="VIO83_05960"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020193574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HEY8330852.1"
                     /translation="MSKRPYKVALNGYGRIGRCVLRALYERGADAGLEIVALNDLTDQ
                     ASIEYLTRFDSTHGRFPGEVKVVGDCLHINGDCVKVLREATPEGIDWAALGVDLVLEC
                     SGVYQTRADAERFIKAGAPRVLFSQPMASEADIDATIVYGVNQQRLSGAERLVSNASC
                     TTNCGVPLLKILDEAIGLDYVSITTIHSAMNDQPVIDAYHHEDLRRTRSAFQSVIPVS
                     TGLARGIERLLPELAGRIKAKAVRVPTVNVSCLDITLQTRRDTSVSEINRVLREAAES
                     GPLKGLLDYTELPHASCDFNHDPHSAIVDGSQTLVSGPRLVNLLAWFDNEWGFANRML
                     DVAEHFLRVAHCPQPMKD"
     gene            7761..8921
                     /locus_tag="VIO83_05965"
     CDS             7761..8921
                     /locus_tag="VIO83_05965"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY8330853.1"
                     /translation="MTVLKMSDLDLAGKRVLIREDLNVPVKDGVVKSDARILASLPTI
                     KLALEKGAAVMVCSHLGRPTEGEFSAENSLKPVAEYLSKALGREVPLVADYLGGVDVQ
                     PGQIVLFENVRFNKGEKKNSDELAQQYAALCDVFVMDAFGTAHRAEGSTHGVAKFAQV
                     AAAGPLLAAELDALGKALGNPARPMVAIVAGSKVSTKLDVLTSLADICDQLIVGGGIA
                     NTFLAAAGYKVGKSLCEAELLDTARAIAAKVSVPLPVDVVVAKEFAETAAATVKLIAD
                     VADDDMILDIGPQTAAMFGEMLKASKTILWNGPVGVFEFDQFGNGTKVLAQAIAESPA
                     FSIAGGGDTLAAIDKYGVAEQISYISTGGGAFLEFVEGKVLPAVAVLEERAK"
     gene            9086..>9210
                     /locus_tag="VIO83_05970"
     CDS             9086..>9210
                     /locus_tag="VIO83_05970"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY8330854.1"
                     /translation="MALISMRQMLDHAAEFGYGVPAFNVNNLEQMRAIMEAADKT"
BASE COUNT         1449 a         3114 c         3151 g         1496 t
ORIGIN      
        1 tgcgcttcag caacggcgtc gaacgcacct acgagcggct ggtcaatcgc ggcctgggct
       61 acggcgcggt gatggtggtg gccttggaag acgcggagac cgtgctgctg atcgaggagt
      121 actgcgccgg tatcgatgcc tacgagctgt cgctgcccaa ggggctgatc gagccgggcg
      181 aggacgtgct ggcggcggcc aatcgcgagc tgaaggagga ggccggcttt ggcgcccgcc
      241 agctcgagca cattaccggc ctgtcgctgt cgccgggcta catgagccag tcgattcagg
      301 tggtgctggc gcgcgacctt tatgccgagc gcctgcctgg cgacgagccg gagagcatcc
      361 gcgtcgagcg cttcagtctg cgcgacatct cgcgtctggc cgcggccgac aacttcagcg
      421 aaggccgcgc cctggccgcg ttgtatctgg tgcgcgacct gctgacccag cgtggcgagt
      481 atcagccgtg agcccagcca tgagccatcc cttgttgccg gcggtggtcg agctggtgcg
      541 tcaggccggc gctgccaccc tgccgtactg gcgcgcggac gtggcggtcg agctcaaggc
      601 cgacgcctcg ccggtgaccg ctgccgacct ggcggcccac cgcctgctgg cggctggcct
      661 ggcgcagctg gcgccggaga ttcacctgct gtccgaggaa gactgcgaga tcggcctggc
      721 cgagcgccgc cgctgggcgc gctggtggct ggtcgacccg ctggacggca ccaaggagtt
      781 catcgccggc agcgaggagt tcaccgtcaa cgtggcgctg atcgagcagg gcaaggtggt
      841 gttcggcgtg gtcggcgtgc cggcgcgtgg cctgtgcttc tacggcggtg ccggcctcgg
      901 cgcctggcgt atggcggatg gcgaggcgca ggcgatcggt gtgcgcatcg ccccggcgga
      961 agccttcacc gtggtggcca gccgtcgcca ctccagcccg gcccaggagc ggctgctcag
     1021 cgggctgggc gagcgcttcg gcgatctggc gctggccagc gtcggcagtt cgctgaagtt
     1081 ctgcctgctc gccgagggca gcgccgactg ctatccgcgg ctggtgccta cttcgcagtg
     1141 ggataccgcc gcggcgcagg gcgtgctgga gggcgccggc ggcgaggtac tcgacctgca
     1201 tggccagccg ctggactacc cggcgcgcga gagtttcctc aacccgcact tcctagccct
     1261 gccgcaggcg gcggagtggc gcgaggaact gctgcagctg gccctggcgc tggattgaga
     1321 ggccggcgct ccgccggctt ggtagggtag aaaaccgctg cgcgggtttt ctaccctacg
     1381 gctaagcccg tagggtgcgc catgcgcacc cggcgccctg ggctcgggcg ctgcctactt
     1441 gtgcagcaca tactccccat gcagatgcac cgccgtctcg ccgctcggtg cgaggatgcg
     1501 gatgtccaga tacagccgcg cgcggccgcg gcggcggtac atcgtttcga acttcttcca
     1561 gtcctcctcg ctcggcgcgg cgcagatggc cacggcctcc gcgaccaccg gcagcggata
     1621 gtcgatctgc ccgcccttga tcacgatatg gccgtcgtcg atgccggcct cgcgcaggcg
     1681 cagggtcagc cagccccagc cggccagcac gctgaggctg tacaggctgc cgccgaacat
     1741 ggtgccggtg tggttgacgt tgggggccag cggcgcggac aggcgcagct cgtggttttc
     1801 ccagcgctgt accctgagtt ccatggtgcg ggtcagcggg atgtcgtggt gcagcagctc
     1861 ctccagatag cgacagtcgg gattcatgtc cggggtgtcc tggtgcggtg gcaggcaagg
     1921 tcgtaaggcg ccgcgcgggt gcgcagcgga tagctcatga tcccgcctcg ggcgcccggg
     1981 tgcaattgcg taggtggctg ctgcgcccgc tgccgagttg ttcgcgcggg catgaaaaag
     2041 ccccgcacgg ctcgcaccgg cggggctttc gctacatctt tcggtatggt agcgggcgat
     2101 tacagaccgg cagcggcgcg cagcgcctcg gccttgtcgg tcttctccca ggtgaaggtg
     2161 gtgaaggtat cgtcaccgac cgtcacttcc tgcggggtgc ggccgaagtg gccgtaggcg
     2221 gcagtggcct tgtacatcgg gtgcagcagg tcgagcatct tggtgatcgc gtacggacgc
     2281 aggtcgaaga cttcgcgcac cagcttgatg atggtgtctt cgctgacctt gttggtgccg
     2341 aaggtgttga tcgagatgga ggtcggctgg gccacgccga tggcgtagga cacctggatc
     2401 tcgcagcgct cggccaggcc ggctgcgacg atgttcttgg ccacgtagcg gccggcatag
     2461 gcggccgaac ggtcgacctt ggacgggtcc ttgccggaga aggcgccgcc gccgtgacgg
     2521 gccatgccgc cgtaggagtc gacgatgatc ttgcggccgg tcaggccgca gtcgcccacc
     2581 gggccgccga tgatgaactg gccggtcggg ttgatgtgga actgggtgtc cttgtgcagc
     2641 agctcggccg gcagggtgtg cttgacgatc agctccatga cggcttcatg caggtcggcc
     2701 tggctgatct ccgggctgtg ctgggtggac agcaccacgg cgtcgatgcc gaccaccttg
     2761 ccgttctcgt agcggcaggt gacctggctc ttggcgtccg ggcgcagcca cggcagcagg
     2821 ccgctcttgc gtgcctcggc ctggcgctcg accaggcggt gcgagaagca gatcggcgcc
     2881 ggcatcagca cgtcggtttc gttgctggcg tagccgaaca tcaggccctg gtcgccggcg
     2941 ccctggtctt ccggcttggc gcggtcgacg ccctggttga tgtccgggga ctgcttgccg
     3001 atgatgttga cgatgccgca ggtggcgccg tcgtagccga cgtcggagct gttgtagccg
     3061 atgtcgatga tcacgtcgcg caccagctgc tcgaggtcga tccaggcgct ggtggtgatc
     3121 tcgccggcga cgatggccac gccggtcttc accagggtct cgcaggccac gcgggcgtgc
     3181 ttgtcctcgg cgatgatggc gtcgagcacg gcatcggaga tctggtcggc gatcttgtcc
     3241 ggatggcctt cggacacgga ctcggaggtg aaaagggaat attcgctcat caatcggttc
     3301 ctgtttttac cggtgggtga gtcggcctcg cggccgtgtc atacagcatt caatcaatgc
     3361 ttcagggtag tgcgggtggc cacttcgggc ttggcgaagt ggcgcacctg gatctggaaa
     3421 ccgttgcgca ggcccaggta caggctttcg cctgcggtca ggccggcgtc ttctgcccag
     3481 cgcgccagct cgtcctggtc gaaacccagc cacaggtcgc cacaggcgtc gcgcgcccag
     3541 gactggtggt ggctgcacag ctcggtcagc agcaggctgc cgcccgcggt cagcaggcgc
     3601 gccagctggg ccatggcccg ggccggctcg gcaaagtggt gcaggaccat gttcagcacc
     3661 acgcagtcgg cgggcgcctg ttcgtcattc aaggcatcgg ccagtttcag ttcgacgttg
     3721 gccaggccct cggcgctgca gcgctggcgg gccagctcga gcatcgccgg gctgctgtcc
     3781 agtgcggtga cgcgcgcgaa gcgccgggcc agctcgggga gaaaggcgcc gtcgccgggg
     3841 ccgacttcca gcgcgctggc actggcgggc aagtgcaggg cgtcgagcag ggtcagcagg
     3901 ctgtcgcggt actgcggcag gccggcgatc aggtcctgct gggcgcggaa cttctccagg
     3961 gtgcgggcga agaagtcgcg cgccgcggcg gcgcgctgcg cctgcacctc ggcgatgcgc
     4021 agctggacct cggccggcag ctgcagtgcg tccacctctt cgagcagcgc tgcctgcagc
     4081 gcgctgccgg tctgcccgct ggcggccagg gcgcggcgat agaagatcgc gttgccttcg
     4141 cggcgggtcg ccaccagccc ggcctgggcc agcaccttga ggtggtggct catccccgac
     4201 tggcgcatgg cgaagatctg cgccagctcc agcacgccga acgagtcgca ggccagcacc
     4261 cgcagcacgt tgaggcgcag cgggtcgccg ccggccttgc acagggcggc caggtcgtcg
     4321 ctgggatcgg ggcggaatgc gggctgggtg ctcataaggg ggcgaagtct aatcgtcgcg
     4381 gcggtgcgtc gcaacttctg tatcaaaaag ttttgatatt ggctgttcgc ggtgccggcg
     4441 agcgccgccc agaccaggcc aattcgcgcg gatcgaccat ctgtgattgc ccgttcccct
     4501 gccgctgggc gaaaatggcc gcctttttga tcaagccccc cctgcaggag accagcgatg
     4561 cccagtcgtc gtgagcgagc caatgccatt cgtgccctca gcatggatgc cgtgcagaaa
     4621 gccaacagcg gccatcccgg tgcacccatg ggcatggcgg acatcgccga agtcctctgg
     4681 cgcgacttcc tcaagcacaa cccgcagaac ccgcagtggg ccgaccgcga ccgtttcgtg
     4741 ctgtccaacg gccacggctc gatgctgatc tactcgctgc tgcacctgac cggctacgac
     4801 ctgtcgatcg acgacctcaa gcagttccgc cagctgcaca gccgcacccc gggccacccg
     4861 gaatacggct acaccccggg tgtggaaacc accaccggcc cgctcggcca gggcatcgcc
     4921 aacgccgtcg gcttcgccat cgccgagaag gtgctgggcg cgcagttcaa ccgtccgggc
     4981 cacgccatcg tcgaccactt cacctacgcc ttcctcggcg acggctgcat gatggaaggc
     5041 atcagccacg aagtctgctc gctggccggc accctcggcc tgggcaagct ggtcgccttc
     5101 tacgacgaca acggcatctc catcgacggc gaagtggaag gctggttcac cgacgacacc
     5161 ccggcgcgct tcgaggccta cggctggcag gtgatccgca gtgtcgacgg ccacgatgcc
     5221 gcggcgatca aggctgccgt cgaggccgcc cgtggcgaaa ccgcgcggcc gaccctgatc
     5281 tgctgcaaga ccaccatcgg cttcggttcg ccgaacaagg gtggcaagga agagtgccac
     5341 ggcgcgccgc tgggcaacga cgagatcgcc gccacccgcg ccgcgctggg ctggaaccac
     5401 gggccgttcg agatcccggc cgacatctac gccgagtggg acgccaaggc cgccggcgct
     5461 gccaacgaag cggagtggaa cgcacgcttc gccgcctacg ccgccgagtt cccgcagctg
     5521 gccgccgagt tccagcgccg catcgccggc gagctgccgg ccgacttcgc cgagcaggcc
     5581 gccgcctacg tcgccgagat cgctgccaag ggcgagacca tcgccagccg caaggccagc
     5641 cagaatgcgc tgaatgcctt cggcccgctg ctgccggaat tcctcggtgg ctcggccgac
     5701 ctggccggct ccaacctgac cctgtggaag ggctgccaga gcctcagcca cgccgacgcc
     5761 tccggcaact acctgcacta cggcgtgcgc gagttcggca tgagcgcgat catgaacggc
     5821 gtcgccctgc acggcggctt cattccctac ggcgccacct tcctgatctt catggaatac
     5881 gcgcgcaacg ccgtacgcat gtcggcgctg atgaagcagc gcgtgctcta cgtattcacc
     5941 cacgactcca tcggcctggg cgaggacggc ccaacccacc agccgatcga gcagctggcc
     6001 agcctgcgcg gcacaccgaa cctcgacact tggcgtccgt gcgatgcggt cgagtccgcg
     6061 gtggcctgga agtacgccat cgagcgcaac gacggcccga gtgcgctgat cttcagccgc
     6121 cagaacctgc cgcaccaggc gcgcgacgcc cagcagctgg ccgacgtggc ccgcggcggc
     6181 tacgtgctca aggactgcgc cggcgagccg gaactgatcc tgatcgccac cggttcggaa
     6241 atcggcctgg ccgtgcaggc cttcgacgcg ctgaccgccc agggccgcaa ggtgcgcgta
     6301 gtgtcgatgc cgtcgaccag cgtgttcgac cagcaggacg ccgcctacaa gcaggccgtg
     6361 ctgccggtgc aggtcggcgc gcgcatcgcc atcgaggccg cgcatgccga cttctggtac
     6421 aagtacgtcg gtctggaagg ccgcatcatc ggcatgacca ccttcggcga atcggcgccg
     6481 gcggcgcagc tgttcgagga attcggcttc accctcgaca acgtgctgag caccgccgaa
     6541 gagctgctgg acgtctgatc ggccattgta ggagcgagct tgctcgcgat tcggcccgcg
     6601 ctgatcgcga gcaagctcgc tcctacagga ttgcccgccc gtaggatgga aacccgcgaa
     6661 gcggttttcc acctgtcgcc acccacagag ctgccatgtc gaaacgcccc tacaaagtcg
     6721 ccctcaacgg ctacggccgc atcggccgct gcgtgctgcg cgcgctgtac gagcgcggcg
     6781 cggatgccgg gctggaaatc gtcgcgctca acgatctgac cgaccaggcc agcatcgagt
     6841 acctgacccg cttcgactcc acccacggcc gcttccccgg cgaggtgaag gtggtcggcg
     6901 actgcctgca catcaacggc gactgcgtga aggtgctgcg cgaggcgacg ccggagggca
     6961 ttgactgggc ggcgctgggc gtcgatctgg tgctggaatg ctccggcgtc taccagaccc
     7021 gtgccgacgc cgagcgcttc atcaaagccg gtgcgccgcg ggtgctgttc tcccagccga
     7081 tggccagcga ggccgacatc gacgccacca tcgtctacgg ggtcaaccag cagcgcctga
     7141 gcggtgccga gcggctggtg tcgaatgcgt cctgcaccac caactgcggc gtgccgctgc
     7201 tcaagatcct cgacgaggcg atcggcctcg actacgtgtc gatcaccacc atccactcgg
     7261 cgatgaacga ccagccggtg atcgacgcct accaccacga ggacctgcgc cgcacccgtt
     7321 cggcgttcca gtcggtaatc ccggtgtcga ccggcctggc ccgtggcatc gagcgcctgc
     7381 tccccgaatt ggccggacgg atcaaggcca aggcggtgcg tgtgccgacg gtcaacgtgt
     7441 cgtgcctcga catcaccctg cagacccgtc gcgacacctc ggtgtccgag atcaaccgcg
     7501 tgctgcgcga ggcggccgag agcggcccgc tcaaggggct gctcgactac accgaactgc
     7561 cccacgccag ctgcgatttc aatcacgatc cccattcggc catcgtcgat ggcagccaga
     7621 ccctggtttc cggcccccgg ctggtcaacc tgctggcctg gttcgacaat gaatggggtt
     7681 ttgccaaccg tatgctcgac gtggcggaac acttcctccg cgtcgcccat tgcccacagc
     7741 ccatgaagga ctgaaagccc atgaccgttt tgaagatgtc cgacctcgac ctcgccggca
     7801 agcgcgtgct gatccgtgaa gacctcaacg tcccggtgaa ggacggtgtg gtgaagagcg
     7861 acgcccgcat cctcgcctcc ctgccgacca tcaagctggc gctggagaag ggtgccgcgg
     7921 tgatggtctg ctcgcacctg ggccgcccga ccgaaggcga gttctccgcc gagaacagcc
     7981 tcaagccggt cgccgagtac ctgagcaagg ccctcggccg cgaagtgccg ctggtcgccg
     8041 actacctggg cggcgtcgac gtgcagccgg gccagatcgt gctgttcgag aacgtgcgct
     8101 tcaacaaggg cgagaagaag aacagcgacg agctggccca gcagtacgcc gcgctgtgcg
     8161 acgtgttcgt catggacgcc ttcggcaccg cccaccgcgc cgagggctct acccacggcg
     8221 tggccaagtt cgcccaggtc gccgcggccg gcccgctgct ggccgccgaa ctggacgcgc
     8281 tgggcaaggc gctgggcaac ccggcgcggc cgatggtggc gatcgtcgcc ggctccaagg
     8341 tgtcgaccaa gctcgacgtg ctgacctcgc tggccgacat ctgcgaccag ctgatcgtcg
     8401 gcggcggcat cgccaacacc ttcctcgccg cggccggcta caaggtcggc aagtcgctgt
     8461 gcgaagccga gctgctggat accgcccgcg ccatcgccgc caaggtcagc gtgccgctgc
     8521 cggtggacgt ggtggtggcc aaggagttcg ccgaaacggc tgcagcgacg gtcaagctga
     8581 tcgccgatgt ggccgacgac gacatgatcc tcgacatcgg cccgcagacc gcggcgatgt
     8641 tcggcgagat gctcaaggcg tcgaagacca tcctgtggaa cggcccggtc ggcgtgttcg
     8701 agttcgacca gttcggcaac ggcaccaagg tgctggccca ggccatcgcc gaaagccccg
     8761 ccttctccat cgccggcggc ggcgacaccc tggcggccat cgacaagtac ggcgtggccg
     8821 agcagatctc ctatatttcc accggcggcg gcgccttcct cgagttcgtc gagggcaagg
     8881 tcctgccggc cgtggcggta ctggaagagc gcgccaagta atgcgtaggg tggacaacgg
     8941 cgcagccttg tccaccatgg ggcggatcgg tggacaagcg cggtgcgcgt tgtccaccct
     9001 acgccgtccc ccgaaccctt gaagccggcc tgcccctgcg gcaggctggg caccgatcaa
     9061 acgaccctta ctgggagcac cacagatggc acttatcagc atgcgccaga tgctggatca
     9121 cgccgccgaa ttcggctacg gcgtgccggc cttcaacgtc aacaacctcg agcagatgcg
     9181 cgccatcatg gaagcggccg acaagaccga
//
LOCUS       DATCUN010000147         9134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_194024, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000147 DATCUN010000000
VERSION     DATCUN010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9134
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194024"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VIO83_05975"
     CDS             complement(<1..413)
                     /locus_tag="VIO83_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003282110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEY8330855.1"
                     /translation="MSASAFFCLLDHEGVLAVHGQDAAKFLQGQLTCNLHYLNAETSS
                     LGARCTPKGRMVSSFRILPLADGFALAMARELVEVQLADLKKYAVFFKAQLADESAAW
                     VRFGLSEGDGALLALGLDLPQEAERVARTGELLAVR"
     gene            545..802
                     /locus_tag="VIO83_05980"
     CDS             545..802
                     /locus_tag="VIO83_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEY8330856.1"
                     /translation="MTEENIELKRLQWECRRGMLELDVLFKPFLDEAFPQLDAADQER
                     FRQLLKCEDPDLFSWFMGYSVPENPDHARMIRLILDRVQPK"
     gene            786..1265
                     /locus_tag="VIO83_05985"
     CDS             786..1265
                     /locus_tag="VIO83_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HEY8330857.1"
                     /translation="MSSRSEPFECHWRPSRRLLALYLAALGLALLAMATAAVPGWARV
                     AGALLCLGHAAWTLPRAVLLSHPAAFTGLRHDRQGWQLRNAAEGWVAVQLRPGSLALP
                     QAVILQFRRPGQWFARGLCIPADSLERDWHRRLRVRLKFSRRRWSAVADAAVDQLGK"
     gene            complement(1250..2869)
                     /gene="nadB"
                     /locus_tag="VIO83_05990"
     CDS             complement(1250..2869)
                     /gene="nadB"
                     /locus_tag="VIO83_05990"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEY8330858.1"
                     /translation="MSQHYQHDVLVIGSGGAGLSLALNLPAHLRVAVLSKGDLAGGST
                     YWAQGGVAGVLDDGDDIESHVQDTLTAGAGLCREDAVRFTVEHSREAIQWLIDQGVPF
                     TPDEEHHEGDRGFAFHLTREGGHSHRRIIHAADATGTAIFNTLLARARQCPNIELLEQ
                     RVAVDLITERKLGGKGRRCLGAYVLNRTTGEVDTFCARFVVLATGGAAKVYLYTSNPD
                     GTCGDGIAMAWRAGCRVGNLEFNQFHPTCLYHPQAKNFLITEALRGEGGLLKLPNGER
                     FMERFDPRAELAPRDIVARAIDHEMKRLGIDCVYLDISHKPTEFIKNHFPTVYERCLQ
                     YGIDITRQPIPVVPAAHYTCGGVVVDSRGHSDVPGLYAIGETSFTGLHGANRLASNSL
                     LECFVYARSAAADIVSQLDRVAMPQQLPTWDASQVTDSDEDVIIAHNWDELRRFMWDY
                     VGIVRTNKRLQRAQHRVRLLLSEIDEFYSNYKVSRDLIELRNLAQVADLMIQSAMLRK
                     ESRGLHFTLDYPGQLAKPGDTILVPPGYLPS"
     gene            3198..3779
                     /gene="rpoE"
                     /locus_tag="VIO83_05995"
     CDS             3198..3779
                     /gene="rpoE"
                     /locus_tag="VIO83_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEY8330859.1"
                     /translation="MQTQESDQQLVERVQRGDKRAFDLLVLKYQHKILGLIVRFVHDP
                     QEAQDVAQEAFIKAYRALGNFRGDSAFYTWLYRIAVNTAKNHLVARGRRPPDSDVSAE
                     DAEFFEGDHALKDLETPERALLRDEIEATVHRTIQQLPEDLRTALTLREFEGLSYEEI
                     ATAMQCPVGTVRSRIFRAREAIDKALQPLLHEG"
     gene            3810..4376
                     /locus_tag="VIO83_06000"
     CDS             3810..4376
                     /locus_tag="VIO83_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RseA family anti-sigma factor"
                     /protein_id="HEY8330860.1"
                     /translation="MSREVLGESVSAVMDGEADELELRRVLAAAGEDPEVRARWGRYQ
                     LARSVMHKQTVMPGLDLAAAVSAAIAAEAVAAPVVQAKSPWQQIGRLAVAASVTLAVL
                     AGVRFYNSQDDAAGMAPQLAQQVPPAAQVQPVLPVVSAPQGPAVLASFPAPAAEESAK
                     QPAADEAQILREVPRKVEAAPVDQAESR"
     gene            4388..4852
                     /locus_tag="VIO83_06005"
     CDS             4388..4852
                     /locus_tag="VIO83_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003293587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HEY8330861.1"
                     /translation="MIEESGRVVSIEDGAVWVETLRRSTCSACSANAGCGQGLMEKLG
                     VGQRCGYVRALTDLQLAVGDGVVIGIREDLLLRSSLQVYLLPLLGLFAGAMLAQWLAL
                     PEAFVILLALGGFSAVWWRVRRLSRRSVDDPAMQPVVLRAQLAACAMPVEDC"
     gene            4888..6306
                     /locus_tag="VIO83_06010"
     CDS             4888..6306
                     /locus_tag="VIO83_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY8330862.1"
                     /translation="MRILKRTTIALVAVLAMAQMLVANAQLPEFTELVEQASPAVVNI
                     STRQAIPTQAAGMQQMPDLEGLPPMFREFFERSIPQMPPGQQRKREAQSLGSGFIISA
                     DGYVLTNNHVVADADEIIVRLSDRSELQAKLIGADARTDVALLKVEGKSLPTVKLGKS
                     ETLKVGEWVVAIGSPFGFDHSVTAGIVSAKGRSLPDENYVPFIQTDVAINPGNSGGPL
                     FNLDGEVVGINSQIFTRSGGFMGLSFAIPIDVAMNVADQLKAQGKVNRGWLGVVIQEV
                     SKDLAETFGLDKPAGALVAQVLEDSPGAKGGLRVGDVILALDGKPIVASADLPHLVGN
                     LKPGTNVTLDVVREGARQQLAMAVGALPEEGDAVAGAGAKEGPARSSNRLGVAVVDLT
                     AEQKQALELQGGALIREVQNGPAALIGLRPGDVITHLNNQSIDSAKTFGKVAEALPKN
                     RSVSMRVLRQGRASFITFKLSE"
     gene            6474..8273
                     /gene="lepA"
                     /locus_tag="VIO83_06015"
     CDS             6474..8273
                     /gene="lepA"
                     /locus_tag="VIO83_06015"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY8330863.1"
                     /translation="MSDLSHIRNFSIIAHIDHGKSTLADRFIQICGGLADREMEAQVL
                     DSMDLERERGITIKAHSVTLHYNAQDGKTYQLNFIDTPGHVDFTYEVSRSLAACEGAL
                     LVVDAGQGVEAQSVANCYTAIEQGLEVMPVLNKMDLPQAEPERVKEEIEHIIGIDATD
                     AVACSAKSGMGVIDVLERLVEVIPPPTGEIEAPLQALIIDSWFDNYLGVVSLVRVRHG
                     RVKKGDKILVKSTGKVHQVDSVGVYTPKHTTTADLKAGEVGFIIAGIKEIQGAPVGDT
                     LTLSNTPDVDVLPGFKRVKPQVYAGLFPVSSDDFENFRDALEKLTLNDAALQYEPESS
                     DALGFGFRIGFLGMLHMEIIQERLEREYDLDLITTAPTVVFEVVLKNGDTVYVDNPSK
                     LPDLSSIEEMREPICRANILVPQTHLGSVITLCIEKRGVQRDMHFLGSQVQVSYDLPM
                     NEVVLDFFDRLKSASRGYASLDYGFDRFQAANLVRLDVLINGERVDALALIVHRDNAA
                     YKGRQLVEKMKELIPRQMFDVAIQAAIGGQVVARSTVKALRKNVLAKCYGGDVSRKRK
                     LLEKQKAGKKRMKQVGSVEIPQEAFLAVLKVDS"
     gene            8279..9133
                     /gene="lepB"
                     /locus_tag="VIO83_06020"
     CDS             8279..9133
                     /gene="lepB"
                     /locus_tag="VIO83_06020"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY8330864.1"
                     /translation="MTLNFPLLLVIAVAVCGVLALIDLLFLAPRRRSAIAAYQGSVSQ
                     ADPQVLQTLSREPVLVEYGKSFFPVLALVLVLRSFLVEPFQIPSGSMKPTLEVGDFIL
                     VNKFAYGIRLPVVDSKVIEIGNPQRGDVMVFRYPSDPAVNYIKRVVGLPGDHVRYSSD
                     KRLFVNGQPVAETLLGDEPGSLGSARLFSEKLGEAEHQVRKEMRRYRIEPDREWVVPA
                     GYYFMMGDNRDNSNDSRYWDDPAIPLALHGMVPDRNIVGKAFAVWMSWPEPKLSHLPT
                     FSRVGLIH"
BASE COUNT         1502 a         2817 c         3208 g         1607 t
ORIGIN      
        1 cgcaccgcca gcagctctcc ggtgcgagcc acgcgctcgg cttcctgcgg caggtcaagg
       61 ccgagggcga gcagggcgcc gtcgccctcg ctgaggccga agcgcaccca ggccgcactc
      121 tcgtcggcca gctgggcctt gaagaacacc gcgtatttct tcaggtcggc cagctgcacc
      181 tccaccagct cgcgggccat ggccagggcg aagccgtcgg ccagcggcag gatgcggaaa
      241 ctggagacca tccgtccctt gggcgtgcag cgcgcgccga ggctgctggt ctcggcgttg
      301 agatagtgga ggttgcaggt cagctggccc tggaggaact tggcggcgtc ctggccgtga
      361 acggcgagga cgccctcgtg gtcgagcagg cagaaaaaag cggatgcaga catcgtgtcg
      421 gtcgccggta cggggtcggg gtggccatga tagagctgcc tgccggcgct tgtcagccgc
      481 aggggcaagg ctggcggccg gtcgatgcct cgttataatg ccgggcgatg ccgaggaggc
      541 cgagatgacc gaagaaaata tcgaactgaa gcgactgcag tgggaatgcc ggcgcggcat
      601 gctggagctg gacgtgctgt tcaagccgtt cctggacgag gcttttccgc aactggatgc
      661 ggcggatcag gagcgctttc gccagctgct caagtgcgag gatccggatc tgttcagctg
      721 gttcatgggc tacagcgtgc ccgagaaccc ggatcacgcc cgcatgattc gactgatcct
      781 ggatcgtgtc cagccgaagt gagcccttcg agtgccactg gcggccgtcg cggcgcctgc
      841 tggcactcta tctcgcggcg ctgggattgg ccctgctggc catggcgact gccgccgtgc
      901 cgggctgggc gcgtgtcgct ggcgctctgc tgtgcctggg gcatgcagcg tggaccctgc
      961 cgcgcgccgt gctgctgagc catccggcgg ccttcaccgg cctgcgccat gatcggcagg
     1021 gctggcagct gcgcaatgcc gccgagggct gggtggcggt gcagttgcgt ccgggcagtc
     1081 tggccttgcc gcaggcggtg atcctgcagt ttcgccggcc ggggcagtgg ttcgcccgcg
     1141 gtctgtgcat tcccgccgac agcctggagc gggactggca ccgccgtctg cgcgtgcgcc
     1201 tgaagttcag ccgccggcgc tggtcggcgg tcgccgatgc ggctgtcgat cagctgggca
     1261 aatagcccgg cgggaccagg atggtgtcgc ccggcttggc cagttgcccc ggatagtcca
     1321 gggtgaagtg caggccgcgg ctctctttgc gcagcatggc cgactggatc atcaggtcgg
     1381 cgacctgagc caggttgcgc agctcgatca agtcacggct gaccttgtag ttgctgtaga
     1441 actcgtcgat ctcgctgagc agcagacgca cgcggtgctg ggcgcgctgc aggcgcttgt
     1501 tggtgcgcac gatgccgacg tagtcccaca tgaagcgccg cagctcgtcc cagttgtgcg
     1561 cgatgatcac gtcctcgtcg gagtcggtga cctggctggc gtcccaggtc ggcagctgct
     1621 gcggcatggc cacccggtcc agctggctga cgatgtcggc cgccgccgag cgggcgtaga
     1681 cgaaacactc cagcagcgag ttgctggcca gccggttggc gccgtgcagg ccggtgaagc
     1741 tggtctcgcc gatggcgtac aggccgggca cgtcgctgtg gccgcggctg tcgaccacca
     1801 cgccgccaca ggtgtagtgc gcagccggca ccaccgggat cggctggcgg gtgatgtcga
     1861 tgccgtattg caggcagcgc tcgtagacgg tggggaagtg gttcttgatg aactcggtcg
     1921 gcttgtggct gatgtccagg tacacgcagt cgatgccgag gcgtttcatc tcgtggtcga
     1981 tggcgcgcgc gactatgtcg cgcggggcca gctcggcgcg cgggtcgaag cgttccatga
     2041 agcgttcgcc gttgggcagt ttgagcaggc cgccttcgcc gcgcagcgct tcggtgatca
     2101 ggaaattctt cgcctgcggg tggtacaggc aggtcgggtg gaactggttg aactccaggt
     2161 tgccgacccg gcagccggcg cgccaggcca tggcgatgcc gtcgccgcag gtgccgtccg
     2221 gattgctggt gtacaggtag accttggcgg cgccaccggt ggccaggacc acgaagcgcg
     2281 cgcagaaggt gtcgacctcg ccggtggtgc ggttcagcac gtaggcgccg aggcagcgcc
     2341 ggcccttgcc gccgagcttg cgctcggtga tcaggtcgac cgccacacgc tgctcgagca
     2401 gctcgatgtt cgggcattgc cgcgcccggg ccagcagggt gttgaagatc gccgtgccgg
     2461 tggcgtcggc ggcatggatg atgcgccggt ggctgtggcc gccctcgcgg gtcaggtgga
     2521 aggcgaagcc gcggtcgccc tcgtggtgtt cctcgtcggg ggtgaagggc acgccctgat
     2581 cgatcagcca ctggatcgcc tcgcggctgt gttcgacggt gaagcgcacc gcgtcctcgc
     2641 ggcacaggcc ggcaccggcg gtcagggtat cctgcacgtg cgattcgatg tcgtcgccgt
     2701 cgtcgagtac cccggccacg ccgccctgcg cccagtaggt cgagccgccg gccaggtcgc
     2761 ccttgctcag cacggccacg cgcaggtggg caggcaggtt gagggccagg gacaggccgg
     2821 cgccgccgct gccgatgacg aggacgtcgt gctgatagtg ttggctcatg ggcgccgttc
     2881 cgctgaatgt cgtccctagt atatagaagg ggcaggcggc acaatataac tgaacttgaa
     2941 aggatatctg cgctcttagc aaggttgccc gccgggcaat cagcgggtgt gtcgcgtcct
     3001 gccatgcgtt tttttacagc gcagattcaa cctcgcgggg tgcaccggca cccggcacga
     3061 attccgtacc cccagcctcc gcggtctcgg ggaaaagctg ttcgcagaga cgaacttttg
     3121 tgcagggccc gagtctatta agcaggttgg gctcactggg tcgcgcaaag gctctgcgga
     3181 cgttcagagg agcatgaatg caaacccagg aatcggatca gcagctggtc gagcgggtcc
     3241 agcgtggtga caagcgtgcg ttcgatctgc tggtgctcaa gtaccagcac aagatactcg
     3301 ggctgatcgt gcgcttcgtg cacgatcccc aggaagccca ggatgtggcg caggaagcct
     3361 tcatcaaggc ctaccgggcg ctgggcaatt tccgcggtga cagcgctttc tacacctggc
     3421 tgtaccgcat tgcggtgaac acggcgaaga atcatctggt ggcgcgtggt cggcggccgc
     3481 cggacagcga tgtcagcgcc gaggatgccg agtttttcga aggcgatcat gccctcaagg
     3541 atctcgaaac accggagcgc gcgctgctgc gcgatgagat cgaggccacg gtgcatcgca
     3601 ctatccagca gttgcccgag gatctgcgca cggccttgac cctgcgcgag ttcgaagggc
     3661 tgagttacga ggaaattgcc actgccatgc agtgtccggt ggggacggtg cgctcgcgga
     3721 tcttccgggc gcgtgaagcg atcgacaagg ccctgcagcc gctgctgcac gagggctgaa
     3781 aacccggcga aagccaagag aggaaaacca tgagtcgtga agtcttgggg gaatcggtat
     3841 ccgccgtgat ggacggtgag gccgatgaac tggagctgcg tcgcgtgctc gcggctgccg
     3901 gggaggatcc cgaggttcgc gcgcgctggg ggcgttacca gctggcgcgt tcggtgatgc
     3961 acaagcagac cgtgatgccg gggctggatc tggctgcagc ggtgtccgcg gccatcgccg
     4021 ccgaagcggt tgcggcgccg gtcgttcagg ccaagagtcc gtggcagcag atcggccgtc
     4081 tggcggtggc cgcttcggtg actctggccg tgctggccgg cgtgcgcttc tacaacagcc
     4141 aggacgatgc tgccggcatg gcaccgcagc tggcccagca ggttccgccg gccgcacagg
     4201 tacagccggt gctgccggtg gtgagtgcgc cgcaaggtcc ggccgtgctg gccagttttc
     4261 cggcgcctgc cgccgaggag tcggcgaagc agccggcagc cgacgaggcg cagatcctcc
     4321 gcgaagtgcc gcgcaaggtc gaggcagcgc ccgtcgatca ggcggagtcg cgctgagtgc
     4381 cgcgggcatg atcgaggagt cgggacgggt tgtctcgatc gaggatgggg cggtgtgggt
     4441 ggaaaccctg cgccgcagta cctgctccgc ctgctcggcg aatgccggct gcggtcaggg
     4501 gctgatggag aagctggggg tggggcagcg ctgtggttat gtgcgggcat tgaccgatct
     4561 gcagctggcc gtcggcgacg gtgtggtcat cggcattcgc gaggatctgc tgctgcgcag
     4621 ctccctgcag gtctacctgc tgccattgct gggtctgttt gccggcgcca tgctggcgca
     4681 atggttggcg ttgcctgaag cttttgtcat tctgctggcc ctcggcgggt tttccgcggt
     4741 gtggtggcgg gtgcgcaggc tcagtcggcg gagcgtggac gatcccgcca tgcagccagt
     4801 ggttctgcgt gcgcagctgg ccgcctgtgc catgcctgtg gaagactgct gatctccgtt
     4861 gtcgatctca aagggagtcc aagattcatg cgtatcctga aacgcaccac gattgcgctg
     4921 gtcgctgtgc tggcgatggc ccagatgctg gtcgccaacg cccagctgcc cgagttcacc
     4981 gagctggtcg agcaggcttc gccagcggtg gtgaacatca gtacccggca ggccatcccg
     5041 acccaggccg ccggcatgca gcagatgccg gacctggaag gcctgccgcc gatgttccgc
     5101 gagttcttcg aacgcagcat cccgcagatg ccgccgggac agcagcgcaa gcgcgaggcg
     5161 caatcgctgg gctctggctt catcatttct gccgacggct atgtgctgac caacaatcac
     5221 gtggtggccg acgccgacga gatcatcgtg cggctgtccg accgcagcga actgcaggcc
     5281 aagctgatcg gtgccgacgc gcgcaccgac gtggccttgc tcaaggtcga gggcaagagc
     5341 ctgccgaccg tcaagctggg caagtcggag acgctgaagg tgggcgagtg ggtggtggcc
     5401 atcggctcgc cgttcggctt cgatcactcg gtcaccgccg gtatcgtcag tgccaagggg
     5461 cgcagcctgc cggacgagaa ctatgtgccg ttcatccaga ccgacgtggc gatcaatccc
     5521 ggcaactccg gtggtccgct gttcaacctg gatggcgagg tggtgggcat caactcgcag
     5581 atcttcaccc gttccggcgg cttcatgggg ctgtcgttcg ccattccgat cgatgtggcg
     5641 atgaacgtcg ccgatcagct caaggcgcaa ggcaaggtca atcgcggctg gctgggtgtg
     5701 gtgattcagg aagtcagcaa ggatctggcc gagaccttcg ggctggacaa gccggccggc
     5761 gcgctggtgg cccaggtgct ggaagacagc ccgggcgcca agggtggcct gcgcgtgggc
     5821 gacgtgatcc tcgccctcga cggcaagccg atcgtggcgt cggccgacct gccgcacctg
     5881 gtcggcaacc tcaagccggg caccaatgtg acgctggatg tggtgcgcga aggcgcccgc
     5941 cagcagctgg cgatggcggt gggtgcgctg ccggaggagg gtgatgcggt cgctggggcg
     6001 ggtgccaagg aggggccagc tcgcagcagc aatcgtctcg gggtggcggt ggtcgacctc
     6061 accgcggagc agaagcaggc ccttgagctg cagggtggtg cgctgattcg cgaggtgcag
     6121 aatggccctg cggcgctgat cggcctgcgt ccgggggatg tcatcaccca cctgaacaac
     6181 cagtcgatcg attcggccaa gaccttcggc aaggtggccg aggcgttgcc gaagaaccgt
     6241 agcgtgtcga tgcgcgtgct gcgtcagggg cgcgccagct tcatcacctt caagctgtcc
     6301 gagtaaggac ggccggaggt gcacaaaggg gcgaagaatt cgcccctttg tcgtttctgc
     6361 ggcagcgtcc ccgtcggcat ccaccgaacc gtgggtgacc ccgccctggc gatcaggtaa
     6421 actccccggc tattttcggc gggctcggcc cgcgctccct gagtgttgag cccgtgagtg
     6481 atctgagcca tatccgcaat ttctccatca ttgcccacat cgaccatggc aagtcgaccc
     6541 tggccgaccg cttcatccag atctgcggcg gcctcgccga tcgcgagatg gaggcccagg
     6601 tgctggactc catggatctc gagcgcgagc gcggcatcac catcaaggcg cacagcgtga
     6661 ccctgcacta caacgcgcag gacggcaaaa cctaccagct gaacttcatc gatacccccg
     6721 gccacgtcga cttcacctat gaagtcagcc gctcgctggc ggcctgcgag ggtgcgctgc
     6781 tggtggtgga cgccggccag ggcgtcgagg cgcagtcggt agccaactgc tacaccgcca
     6841 tcgagcaggg tctcgaggtg atgccggtac tcaacaagat ggacctgccc caggccgagc
     6901 cggagcgggt caaggaagag atcgagcaca tcatcggcat cgacgccacc gacgccgtgg
     6961 cctgcagcgc caagagcggc atgggcgtga tcgacgtgct cgagcgcctg gtcgaggtga
     7021 ttccgccgcc gaccggcgag atcgaggcgc cgctgcaggc gctgatcatc gactcctggt
     7081 tcgacaacta cctgggcgtg gtctcgctgg tgcgcgtgcg ccatggccgg gtcaagaagg
     7141 gcgacaagat cctggtcaag tcgaccggca aggtccacca ggtcgacagc gtcggcgtgt
     7201 acacgcccaa gcacaccacc accgccgacc tcaaggccgg cgaagtgggc ttcatcatcg
     7261 ccggtatcaa ggagatccag ggcgcgccgg tcggcgatac cctgaccctg tccaataccc
     7321 cggacgtcga cgtgctgccc ggcttcaagc gggtcaagcc gcaggtctac gccggcctgt
     7381 tcccggtcag ctcggacgac ttcgagaatt tccgcgatgc gctggaaaag ctcaccctca
     7441 acgacgccgc gctgcagtac gagccggaaa gctcggatgc gctgggcttc ggcttccgca
     7501 tcggcttcct cggcatgctg cacatggaga tcatccagga acgcctcgag cgcgagtacg
     7561 acctggacct gatcaccacc gcgccgaccg tggtgttcga ggtggtgctg aagaacggcg
     7621 acaccgtgta cgtcgacaac ccgtccaagc tgcccgacct gtcgtcgatc gaggagatgc
     7681 gcgagccgat ctgccgcgcc aatatcctgg tgccgcagac ccatctgggc agcgtgatca
     7741 ccctgtgcat cgagaagcgc ggcgtgcagc gcgacatgca cttcctcggc agccaggtgc
     7801 aggtcagcta tgatctgccg atgaacgagg tggtgctcga cttcttcgac cgcctgaagt
     7861 cggccagccg cggctatgct tcgctggact acggtttcga ccgcttccag gccgccaacc
     7921 tggtgcgcct ggacgtgctg atcaacggcg agcgggtcga tgccctggcg ttgatcgtcc
     7981 accgtgacaa cgcggcctac aagggccgtc agttggtgga gaagatgaag gagctgatcc
     8041 cgcggcagat gttcgacgtg gcgatccagg cggccatcgg cggccaggtt gtcgcccgct
     8101 cgacggtcaa ggcactcagg aagaacgtgc tggccaagtg ctacggcggc gacgtcagcc
     8161 gcaagcgcaa gctgctggag aagcagaagg ccggtaagaa aaggatgaag caggtaggca
     8221 gtgtggaaat tccgcaggaa gccttccttg ctgtgctcaa ggtggacagc taaggctcat
     8281 gactctgaac tttccgttgt tgctggtgat tgccgtcgcc gtgtgcggcg tgctcgcgct
     8341 gatcgacctg ctgttcctcg ccccgcgccg gcgctcggcc attgctgcct accagggcag
     8401 cgtgagccag gccgatccgc aggtgctgca gacgctgtca cgcgagccgg tactggtgga
     8461 atacggcaag tcgttcttcc cggtgctggc gctggtgctg gtgctgcgct cgttcctggt
     8521 cgagccgttc cagatcccgt cgggctcgat gaagccgacc ctcgaggtcg gcgacttcat
     8581 cctggtcaac aagttcgcct atggtatccg tctgccggtg gtcgacagca aggtgatcga
     8641 gatcggcaac ccgcagcgcg gcgatgtgat ggtgttccgc tacccgagcg atccagccgt
     8701 gaactacatc aagcgcgtgg tcggcctgcc gggcgatcat gtgcgctaca gcagcgacaa
     8761 gcgtctgttc gtcaacggtc agccggtggc cgagacgctg ctcggcgacg agcccggcag
     8821 cctcggcagt gcccgcctgt tcagcgagaa gctgggcgag gccgagcatc aggtgcgcaa
     8881 ggaaatgcgc cgctaccgca tcgaacctga ccgtgagtgg gtggtgccgg ccgggtacta
     8941 cttcatgatg ggcgacaacc gcgacaactc caacgacagc cgttattggg acgatccggc
     9001 gattccgctt gcgctgcatg gcatggttcc ggatcgcaac atcgtcggca aggcgttcgc
     9061 cgtgtggatg agctggccgg agcccaagct cagccacctg ccgacgttct cgcgggtcgg
     9121 gctgatccat tgag
//
LOCUS       DATCUN010000148         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_24917, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000148 DATCUN010000000
VERSION     DATCUN010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24917"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VIO83_06025"
     CDS             complement(<1..294)
                     /locus_tag="VIO83_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8330865.1"
                     /translation="MQASFSDLEYVAQQCFGLSDEGIEDAVYDSHAIRRFIGIDLARE
                     AAPDATTLLKFRRLLEDQGLTGVIFETINAHLARQGLLLREGTLVDATLIAAPP"
     gene            complement(440..1246)
                     /locus_tag="VIO83_06030"
     CDS             complement(440..1246)
                     /locus_tag="VIO83_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEY8330866.1"
                     /translation="MTGLLEPYLARLGITGTLSPTPEALLALHRAHALGVPYENLDVQ
                     LGQPLTTDPAAALARLAAGRGGWCYEMNGAFGAALTLLGFSVTRLAGGVQRAALGDAA
                     LGNHLVLKVVIDGAALLCDVGFGDGLLEPIPLQEGHYRQGALGFRLQRLDDGLWRFHG
                     DARTGGAPSFDFADAPADEALLARQCAALQSAPESPFVLNAVVQRHRPDGHVALRGRV
                     LRTLTTDRVRERTLDGPDDYAATLVREFGLELPAAALWPRIVARHAALFG"
     gene            complement(1288..>1791)
                     /locus_tag="VIO83_06035"
     CDS             complement(1288..>1791)
                     /locus_tag="VIO83_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY8330867.1"
                     /translation="LLGSGPRCGIGELYLPENEPGSSIMPGKVNPTQSEALTMVAAQV
                     FGNDVAINFGGASGHLELNVFKPLLIRNLLQSIRLLTDASRSFADHCVAGTVANEPQI
                     AQYLERNLMLVTALNPHIGYDNAAKAAKKAHHENLTLKESVVALGLLSPEDFDRLIRP
                     ADMLGPG"
BASE COUNT          286 a          607 c          609 g          289 t
ORIGIN      
        1 cggcggggcg gcgatcagcg tcgcatccac gagggtgccc tcacgcagca gcaggccctg
       61 ccgggccagg tgcgcgttga tcgtctcgaa gatcacgccg gtcagaccct gatcctccag
      121 caggcggcgg aacttcagca gcgtggtggc atccggcgcc gcctcgcggg ccagatcgat
      181 gccgatgaag cggcggatgg cgtggctgtc gtacacggcg tcctcgatgc cctcgtccga
      241 cagcccgaag cactgctgcg ccacgtactc gaggtcggaa aagctcgctt gcatgacgtc
      301 tgctcaccac ggctgcggac cgggccgcat cgtctcagga ccaaggtctc atgccaccaa
      361 cgggcgggaa taaatcagtg tttcccgata aaccggtgtg ggcacgctgc gctttgccca
      421 ccctgcagct cgctatttct tatccgaaca gtgccgcgtg ccgcgcaacg atccgcggcc
      481 acagcgccgc ggccggcagt tcgagcccga actcacgaac cagcgtcgcc gcatagtcgt
      541 ccgggccgtc cagcgtccgc tcgcgcaccc tgtccgtcgt cagggtgcgc agcacccggc
      601 cgcgcagggc gacatgcccg tccggccggt gccgttgcac cactgcattg agtacgaatg
      661 gcgattccgg ggcgctctgc aaggcggcgc attgccgcgc cagcagggcc tcgtcggccg
      721 gtgcatcggc aaaatcgaac gacggcgcgc cgccggttct ggcgtcgcca tgaaaacgcc
      781 acaggccatc gtcgagacgc tgcaggcgaa agcccagcgc accttgccga tagtggcctt
      841 cctgaagcgg gatcggctcg agcaggccat cgccaaagcc gacgtcgcac agcagggcgg
      901 cgccgtcgat caccaccttg agcaccagat ggttgccgag cgcggcgtca cccagggctg
      961 cccgctgcac gccgccggcc aggcgtgtca ccgaaaagcc gagcagtgtc agcgcggcgc
     1021 cgaaggcacc gttcatttcg taacaccagc cgccgcgccc ggcagccagc ctggcgagcg
     1081 ctgccgcggg gtcggtcgtc agcggctggc ccagctgcac gtcgaggttc tcgtacggca
     1141 cgccgagcgc atgcgcgcgg tgcagtgcta acagcgcttc gggtgtgggc gagagcgtgc
     1201 ccgtaatgcc gaggcgggcg agataaggct ccagaagtcc ggtcatgggc gcttgcattc
     1261 tgttgcgtgc ctgctgacga cgttgcctca gcccggcccc agcatgtccg ccggccggat
     1321 caggcggtcg aaatcctcgg ggctgagcag gccgagtgcg accacggatt ccttgagcgt
     1381 caggttctcg tggtgcgcct tcttggcggc cttggcggcg ttgtcgtagc cgatgtgcgg
     1441 gttgagcgcc gtcaccagca tcaggttgcg ctccaggtac tgggcgatct gcggctcgtt
     1501 tgccacggtg ccggcgacgc agtggtcggc gaaggagcgg ctggcgtcgg tcagcaggcg
     1561 gatcgattgc aacaggttcc tgatcagcag cggcttgaac acgttcagtt ccaggtggcc
     1621 gctggcgccg ccaaagttga tggcgacatc attgccgaac acctgcgccg ccaccatggt
     1681 cagcgcctcg gactgcgtcg ggttgacctt gccgggcatg atcgagctgc ccggttcgtt
     1741 ctccggcagg tacagctcgc cgatgccgca gcgcgggccg gagcccagca g
//
LOCUS       DATCUN010000149         4157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_81031, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000149 DATCUN010000000
VERSION     DATCUN010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81031"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VIO83_06040"
     CDS             complement(<1..688)
                     /locus_tag="VIO83_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEY8330868.1"
                     /translation="MKKILFQLHWLLGISAGLVLALMGVTGASLSYQDELLRLLNPGV
                     MSVEPVVGARPLSPDELVARVGEQLPKRQLLGLTFSADALESVKVNVSGGGPRGETLY
                     ADPFSGDLLGAARGAGLFQFMMQLHRWLAIGDAGKPITGAATLALVFLCLSGLYLRWP
                     RQAGNWRAWLTLDWRRSGRGFLWDLHAVAGTWVLLAYLLASLTGLYWSYEWYRNGVHA
                     LAGEPAAQHGG"
     gene            complement(917..3184)
                     /locus_tag="VIO83_06045"
     CDS             complement(917..3184)
                     /locus_tag="VIO83_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEY8330869.1"
                     /translation="MTRTIERPASAPRVLASAIGAVTALSAVNWAHAEESEALTLDAV
                     SVVDAQEEESYKAESASQKYTAPLRETPKSVTVIPQQVIKDTGALTLTDALRTTSGIT
                     FGAGEGGNPAGDRPIIRGFNAESDTFIDGLRDVASQTREMFNIEQVEVSKGPGSAYTG
                     AGSTGGSLNLISKTAKRNDFLDISQTFGSDQTQRTTLDGNYVFSDNVAGRLNLMKHDA
                     NVAGRDGVDVSRWGVAPSITFGFDTPTRATLSYYHLETDDTPDYGLPLTDATSANSVR
                     KPVSVDRDNFYGLTGRDYRESTTDAGTFRLEHDLNDSLTLSNTTRVVRTTLDYIVTTP
                     NDSRTGNLSQGLVSRSAKSRNSTSEGWVNQTDLKALFNTGSIEHSLVTGVEISREGVH
                     NRNYFITSGTKGNTCTPAHVATGDCTSLSNPSYKDGWTGTVTDSNAFTDTDTDTLAAY
                     VFDTLKFNEQWSLNMGLRYDDFETAASGQTVASGSNNVNGVTDLQSKSHFWNYQLGLV
                     FNPLPNGSIYAAWSTSSNPVGETAGEGASNVAVATADLEPERNRNYEIGTKWDFFDAR
                     LGLNAAIFRTEKSNARVLEADGTTSNVGETRVDGFELGVSGQITPKWNAFASYTYLDG
                     ELVESGRVDVDARPGTGNVNNVVGGADGNDIPSTPQNSFSFWTTYDLTQNLTVGGGAN
                     YVDSRYGDVTNSIEVPSYWRYDAMANYKVSKNLDLQLNVQNLTDKRYFDQVYSNHMAH
                     VAPGRTALLSTNFHF"
     gene            3315..3995
                     /locus_tag="VIO83_06050"
     CDS             3315..3995
                     /locus_tag="VIO83_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEY8330870.1"
                     /translation="MLLHVPGVFSRDEVKRIRQALEQAQWLDGKLTAGYQSAQAKHNL
                     QLAEDDPLAREISAAMLERLWQHPLFMSAALPNKVFPPLFNCYTAGGHFGYHVDNAVR
                     QVRNSAERVRTDLSSTLFFSDPDEYDGGELVIQDTYGSQRVKLPAGDLVLYPSTSIHK
                     VEPVTRGARIASFFWTQSLVREDAQRALLFDMDQAIQSLTRDVPEHPALVQLTGNYHN
                     LLRRWVEV"
     gene            4003..>4157
                     /locus_tag="VIO83_06055"
     CDS             4003..>4157
                     /locus_tag="VIO83_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEY8330871.1"
                     /translation="MSHILQRRETVTPEQLAEDPRQAARWILAAAEAGEPEGQALLGQ
                     ILLDGRG"
BASE COUNT          659 a         1333 c         1422 g          743 t
ORIGIN      
        1 gcccgccgtg ctgcgccgcc ggctcgccgg ccagggcatg cacgccgttg cggtaccact
       61 cgtaggacca gtacagcccg gtcagcgacg ccagcaggta ggccagcaac acccaggtgc
      121 cggccaccgc gtgcaggtcc cagaggaaac cgcggccgct gcgccgccag tcgagggtca
      181 gccaggcgcg ccagttgccg gcctggcggg gccagcgcag gtacaggccg gacaggcaga
      241 ggaacaccag cgccagggtc gccgcgccgg tgatcggctt gccggcgtcg ccgatggcca
      301 gccagcggtg cagctgcatc atgaactgga acaagccggc gccgcgcgcg gcaccgagca
      361 ggtcgccgct gaagggatcg gcgtacaggg tctcgccacg cggaccgccg ccactgacgt
      421 tgaccttgac cgactccagc gcgtcggcag agaaggtcag gccgagcagc tggcgcttgg
      481 gcagctgctc gccgacgcgg gccaccaact cgtccgggct cagcggccgc gcgccgacga
      541 ccggctcgac gctcatcacg ccgggattga gcagacgcag cagctcgtcc tggtaggaca
      601 ggctggcgcc ggtcacgccc atcaacgcca gcaccagccc ggcgctgata cccagcagcc
      661 agtgcagctg gaaaaggatt ttcttgatca cctgggatca cctgatcgct atgccgcggc
      721 tgcggcaaag ttggactcga acgcggcgcg gaaccgcatc gccctgccag ccattgcggc
      781 agcacgaaac gattctattc gagaataatt tgcaacaaat ccactttacc caacctttac
      841 ccgccctgac tccggccaat gagcagcgcg gacagacaaa ggccgcccgc aggcggccct
      901 tgtcgacgcg tcaaagtcag aagtggaagt tggtgctgag cagcgcggta cgtcccggcg
      961 ccacgtgggc catgtggttg gaatagacct gatcgaagta gcgcttgtcg gtcaggttct
     1021 gcacgttgag ctgcaggtcg aggttcttgc tgaccttgta gttggccatc gcgtcgtagc
     1081 gccagtagga cggcacctcg atggagttgg tgacgtcgcc atagcgcgag tcgacatagt
     1141 tggcgccacc gccgaccgtc agattctggg tcaggtcata ggtggtccag aagctgaagc
     1201 tgttctgcgg ggtgctgggg atgtcgttgc cgtcggcgcc gccgacgacg ttgttgacgt
     1261 tgccggtgcc cgggcgcgca tcgacatcca cccggccgct ttcgaccagc tcgccgtcga
     1321 ggtaggtgta gctggcgaag gcgttccact tcggcgtgat ctggccgctg acacccagct
     1381 cgaagccgtc gacacgggtt tcgccgacgt tgctggtggt gccatcagcc tcgagcaccc
     1441 gcgcgttgct cttctcggtg cggaagatgg cggcgttcag ccccagccgc gcgtcgaaga
     1501 agtcccactt ggtgccgatc tcgtagttgc ggttgcgctc cggctccagg tccgcggtag
     1561 ccacggccac gttgctggcc ccttcgccgg cggtttcgcc gaccgggttg ctcgaggtcg
     1621 accaggccgc atagatgctg ccgttgggca gcgggttgaa caccaggccg agctggtagt
     1681 tccagaagtg gctcttgctc tgcagatcgg tcacgccgtt gacgttgttg ctgccgctgg
     1741 cgacggtctg tccgctggcc gccgtttcga agtcgtcgta gcgcagcccc atgttcagcg
     1801 accactgctc gttgaacttg agggtgtcga agacgtaggc ggccagggtg tcggtgtcgg
     1861 tatcggtgaa ggcgttgctg tcggtaaccg tgccggtcca gccatccttg tagctcgggt
     1921 tgctcaggct ggtgcaatcg ccggtggcca catgcgccgg ggtgcaggta ttgcccttgg
     1981 tgcccgacgt gatgaagtag ttgcggttgt gcaccccttc gcggctgatc tccacgccgg
     2041 tgaccaggct gtgctcgatg ctgccggtat tgaacagcgc cttcaggtcg gtctggttga
     2101 cccagccttc ggaggtggag ttgcggctct tcgccgagcg ggagaccaga ccttgcgaca
     2161 ggttgccggt gcgcgagtcg ttcggcgtgg tgacgatgta gtcgagggtg gtgcgcacca
     2221 cgcgggtggt gttggacagg gtcaggctgt cgttgaggtc gtgctcgagc ctgaaggtgc
     2281 cggcgtcggt ggtgctctcg cggtagtcgc ggcccgtcag gccgtagaag ttgtcgcggt
     2341 cgacgctcac cggcttgcgc accgagttgg ccgaagtggc atcggtcagc ggcaggccgt
     2401 agtccggcgt gtcgtcggtt tccaggtggt agtaggacag cgtggcgcgg gtcggcgtgt
     2461 cgaagccgaa ggtgatgctc ggcgccacgc cccagcggct gacatcgacg ccgtcgcggc
     2521 cggcgacgtt ggcatcgtgc ttcatcaggt tcaggcgccc ggcgacgttg tcgctgaaca
     2581 cgtagttgcc gtccagggtg gtgcgctggg tctggtcgga gccgaaggtc tggctgatgt
     2641 cgaggaagtc gttgcgcttg gcggtcttgc tgatcaggtt gaggctgccg ccggtggagc
     2701 cggcgccggt gtaggccgag cccgggccct tgctgacttc cacctgctcg atgttgaaca
     2761 tctcgcgggt ctgcgaggcg acgtcgcgca ggccgtcgat gaaggtgtcg ctctcggcgt
     2821 tgaagccgcg gatgatcggc cggtcgccgg ccgggttgcc accctcgccg gcaccgaagg
     2881 tgatgcccga ggtggtgcgc agcgcatcgg tcagggtcag cgcgccggtg tccttgatca
     2941 cctgctgcgg gatgacggtg accgacttgg gcgtttcgcg cagcggcgcg gtgtacttct
     3001 gcgacgcgga ctcggccttg tagctctcct cctcctgggc gtcgaccacg ctgaccgcat
     3061 ccagcgtcag cgcctcgctc tcctcggcat gagcccagtt caccgcggaa agcgccgtca
     3121 cggcgccgat tgccgaagcc agtacacgcg gcgcagaggc cggccgttcg atagtgcgcg
     3181 tcatcacttc cccctacaga aggtcgattg gttctgttgt ggggcgcact ctaaacgaga
     3241 aatattcttg atatcaagca taatgacact taatatcatt tgcgaaatgt tcgccatcag
     3301 ccacggaggc cggcatgctg ttacatgtac cgggagtatt ttcccgcgat gaggtcaagc
     3361 gcatccgcca ggcgctggaa caggcgcagt ggctcgacgg caagctgacc gccggctacc
     3421 agtcggcgca ggccaagcac aacctgcagc tggccgagga cgatccgctg gcccgcgaga
     3481 tcagcgcggc catgctcgag cgcctgtggc agcacccgct gttcatgtcc gccgcgctgc
     3541 cgaacaaggt gttcccgccg ctgttcaact gctacacggc cggcggccac ttcggctacc
     3601 acgtcgacaa cgcggtgcgc caggtgcgca attccgccga gcgggtgcgc accgacctgt
     3661 cgtccacgct gttcttcagc gacccggacg aatacgacgg cggcgagctg gtgatccagg
     3721 acacctacgg tagccagcgg gtcaagctgc cggccggcga cctggtgctc tacccgtcca
     3781 ccagcatcca caaggtcgag ccggtgaccc gcggcgcgcg catcgcctcg ttcttctgga
     3841 cgcagagcct ggtgcgcgag gatgcccagc gcgccctgct gttcgacatg gaccaggcga
     3901 tccagagcct gacccgcgac gtgcccgagc atccagcgct ggtccagttg accggcaatt
     3961 accacaacct gctgcgccgc tgggtcgagg tctgagccgg ggatgagcca tatcctccag
     4021 cgccgcgaga cagtcacgcc cgagcaactc gccgaagacc cgcgccaggc cgcgcgctgg
     4081 atcctcgccg cggccgaggc cggcgagccg gagggccagg ccctgctcgg ccagatcctt
     4141 ctcgacggcc gaggcat
//
LOCUS       DATCUN010000150         4737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_269078, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000150 DATCUN010000000
VERSION     DATCUN010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269078"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VIO83_06060"
     CDS             <1..521
                     /locus_tag="VIO83_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY8330872.1"
                     /translation="HELFGGGMGPVISRELFVRHDAVCVLPYDPLRDAVVLIEQFRVG
                     ALVKSAHPWLMELVAGLIDKDEVLEEVARREAMEEAGLTLGELWPVTAYYPSPGGSDE
                     RVHLFVGRCDSSGAGGIHGLVEEGEDIRVLVMSLDEALAALAAGRLDNAASIIALQWL
                     ALNREQVRQTWR"
     gene            512..985
                     /locus_tag="VIO83_06065"
     CDS             512..985
                     /locus_tag="VIO83_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HEY8330873.1"
                     /translation="MAMSLLAERYRVDLAGLQATCEANYARLMRLLPGMRERPALRRL
                     ALSAGDATLAVLALKVTEAGPYTSTVEVREEPGLSWLPAPCLEVRVYHDARMAEVTGA
                     EHCRRLQPRYVYPNAAMHQPDEKSQLNLFLGEWLAHYLSCGHLAENPVDHSVVRR"
     gene            1072..1887
                     /gene="cpdA"
                     /locus_tag="VIO83_06070"
     CDS             1072..1887
                     /gene="cpdA"
                     /locus_tag="VIO83_06070"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HEY8330874.1"
                     /translation="MHSTHATPVAAPLLLVQLTDSHLFAEADGRLLGMDTADSLSRVV
                     ELVRAEQPRVDLILASGDLSQDGSFASYQRFAALTAPLDAPARWFAGNHDEAHMLREA
                     CVGTERLQMVTDLGNWRLIALDSSIPGAVPGQLDAAQLQQLEAALDSAGERHVLLTFH
                     HHPVSVGCSWLDPIGLLNPEALFTVVDRYANLRGILWGHVHQEVDRQRNGVRLLASPS
                     TCVQFAPGSEEFQVSSEAPGYRWLRLHADGTLETGVSRVSGIDFVVDYSIKGY"
     gene            2048..2671
                     /locus_tag="VIO83_06075"
     CDS             2048..2671
                     /locus_tag="VIO83_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEY8330875.1"
                     /translation="MSPLPTLIYIHGFNSSPDSLKARELLAAFERLGLTAQLRLPALH
                     HDPRQAIAQLEAEIARAGRPVLVGSSLGGYYATFLAERHGLKALLINPAVRVRRYFAD
                     YLGPQQNFHSGERWQLTGEHVDALAALEVPPPLDAARFTVWLQTGDETLDYRDAADYY
                     RHCTLDIRAGGDHGYQGFAERLPELLAFAGFPARLCRDAEPYGRPQA"
     gene            2731..4626
                     /gene="parE"
                     /locus_tag="VIO83_06080"
     CDS             2731..4626
                     /gene="parE"
                     /locus_tag="VIO83_06080"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016395266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEY8330876.1"
                     /translation="MANTSYTAEAIEVLSGLDPVRKRPGMYTDTSRPNHLAQEVIDNS
                     VDEALAGHAKSVQVILHADNSLQVIDDGRGMPVDIHPEEGVSGVELILTKLHAGGKFS
                     NKNYAFSGGLHGVGISVVNALSNRVEVRVRRDGNEYGMTFADGYKASELEVLGTVGKR
                     NSGTSVQFWPDAKYFDAPKFSISRLKHVLKAKAVLCPGLTVSFEDKTSGEKVEWFYED
                     GLRSYLSDAVSEFARLPEEPFCGSLAGSREAVDWALLWLPEGGESVQESYVNLIPTAQ
                     GGTHVNGLRQGLLDAMREFCEFRSLLPRGVKLAPEDVWERIAFVLSMKMQEPQFSGQT
                     KERLSSREAAAFVSGVVKDAFSLWLNEHPELGLALAELAISNAGRRLKAGKKVERKKI
                     TQGPALPGKLADCAGQDPMRAELFLVEGDSAGGSAKQARDKEFQAILPLRGKILNTWE
                     VDGGEVLASQEVHNIAVAVGLDPGSSDLSQLRYGKICILADADSDGLHIATLLCALFV
                     QHFRPLVEAGHVYVAMPPLYRIDLGKEVWYALDEAERDGILERLLAEKRRGKPQVTRF
                     KGLGEMNPPQLRETTMDPNTRRLVQLTLEDAQGTLEIMDMLLAKKRAGDRKNWLESKG
                     NLAEVLL"
BASE COUNT          755 a         1594 c         1625 g          763 t
ORIGIN      
        1 gccacgagct gttcggcggc ggcatggggc cggtgatcag tcgcgagctg ttcgtgcgtc
       61 acgatgcggt ctgcgtgctg ccctatgatc cgctgcgcga tgccgtggtg ctgatcgagc
      121 agttccgcgt cggcgcgctg gtcaagagcg cgcacccctg gctgatggag ctggtggccg
      181 ggctgatcga caaggacgag gtgctggagg aggtggcgcg ccgcgaggcg atggaagagg
      241 cgggtctcac cctcggcgag ctgtggccgg tgaccgccta ctacccctcg ccgggcggca
      301 gcgacgagcg cgtgcacctg tttgtcggtc gttgcgacag tagcggtgcc ggcggcatcc
      361 acggcctggt cgaggaaggc gaggacatcc gcgtgttggt gatgagcctg gacgaggcac
      421 tggccgcgct ggcggccggg cgtctcgaca atgcggcgag catcattgct ctgcagtggc
      481 tggcgctgaa ccgcgagcag gtgcggcaga catggcgatg agcctgctgg ccgagcgcta
      541 ccgggtcgac ctggccgggc tgcaggccac ctgcgaggcc aattacgcgc ggctgatgcg
      601 cttgctgccg ggcatgcgcg agcggccggc gctgcgccgg ctggcgctga gtgcgggcga
      661 cgctaccctg gcggtgctgg cgctcaaggt caccgaggcc ggcccgtaca ccagcaccgt
      721 ggaagtgcgc gaggagccgg ggctgagctg gctgccggcg ccgtgtctgg aggtgcgcgt
      781 ctatcacgat gcacgcatgg ccgaggtcac cggcgccgag cattgccggc gcctgcagcc
      841 gcgctacgtt tacccgaatg cggccatgca tcaaccggac gaaaagagcc agctcaacct
      901 gttccttggc gaatggttgg cgcactacct gagctgcggc catctggcgg aaaacccagt
      961 ggatcactcg gtcgttcgcc ggtagtgtga accgcatagc ggtcctcgct ggccgcgcat
     1021 gccaacatgc tgattccgca tttgcgggcc catcgccgac aggagacggc tttgcacagt
     1081 acccatgcca cccccgttgc cgcaccgctg ctgctggtgc agctcaccga cagccacctg
     1141 ttcgccgaag cggacggccg tctgctgggt atggataccg cagacagcct gagccgggtg
     1201 gtcgagctgg tgcgtgcgga gcagccgcgg gtcgacctga tccttgccag cggtgacttg
     1261 tcgcaggacg gctccttcgc gtcctaccag cgctttgctg ccctgaccgc gcccctcgac
     1321 gcgccggcgc gctggttcgc cggcaaccat gacgaggcgc acatgctgcg cgaggcctgt
     1381 gtcggcaccg agcgcctgca gatggtcacc gacctcggca actggcggct gatcgccctc
     1441 gactcgagca ttcccggagc ggtgcctggc cagcttgatg ctgcccagtt gcagcaactc
     1501 gaggccgcgc tggacagcgc cggcgagcgc catgtactgc tgaccttcca ccatcatccg
     1561 gtgtcggtgg gctgcagctg gctggacccc atcggcctgc tgaaccccga ggcgctgttc
     1621 accgtggtgg accgctatgc gaatctgcgc ggtatcctct gggggcacgt gcatcaggaa
     1681 gtcgaccggc agcgcaacgg cgtgcgtctg ctggcctcgc cgtccacctg tgtgcagttc
     1741 gctcccggca gcgaggagtt ccaggtcagc agcgaggcgc ctggctatcg ctggctgcgc
     1801 ctacacgccg acggcacgct ggaaaccggc gtgtcgcggg tcagcggcat cgacttcgtc
     1861 gtcgactaca gcatcaaggg ctactgaggc cgaggtgaat gcggcctgct ctgatctgcg
     1921 tggcggtggg ggagtgcgcc gcgtagggtg cgccgcgcgc accctacgac tcgagtgtca
     1981 tccgacttga atccggcggc tggataatgc tctccttgta tatccataca gctgcaaggc
     2041 gccgtcgatg tccccgctgc cgaccctgat ctacattcac ggcttcaaca gctcgcccga
     2101 ctcgctcaag gcccgtgagc tgctcgctgc cttcgaacgc ctggggctga ctgcgcagtt
     2161 gcgcctgccg gcgctgcatc acgatccgcg ccaggcgatt gcccagctcg aggccgagat
     2221 tgccagggca gggcgcccgg tgctggtcgg cagttccctc ggcggctact atgcgacctt
     2281 tctagccgaa cggcatggcc tcaaggccct gctgatcaac ccggcggtgc gcgtgcggcg
     2341 ttacttcgcc gactacctcg gtccgcagca gaacttccac agcggcgaac gctggcagct
     2401 gaccggcgaa catgtcgatg cgctggcggc gctggaagtg ccgccgccgc tggacgcggc
     2461 acgcttcacc gtgtggctgc agaccggcga cgagaccctc gactaccgtg atgcggccga
     2521 ctactaccgg cactgcacgc tggatatccg cgcaggtggc gatcacggct accaggggtt
     2581 cgccgaacgc ctgccggagc tgctggcctt tgccggcttt cccgccaggc tgtgccgcga
     2641 tgccgagcct tacggtcgtc cgcaggcctg accggtcaac gccgccaccc cgttcagctg
     2701 tccccctttt tgccagacag aagtccaccc atggccaata cctcctatac cgcagaagcc
     2761 atcgaagtcc tctccggtct cgatccggtg cgcaagcgcc cgggcatgta caccgacacc
     2821 agccgcccca accacctggc ccaggaagtc atcgacaaca gcgtcgacga ggcgctggcc
     2881 ggtcacgcca agtcggtgca ggtgattctc cacgccgaca actcgctgca ggtgatcgac
     2941 gacggccgcg gcatgccggt ggacatccac ccggaggagg gcgtcagcgg tgtcgagctg
     3001 atcctcacca agctgcacgc cggcggcaag ttctccaaca agaactatgc cttctccggc
     3061 ggcttgcacg gcgtcggtat ctcggtggtc aacgccctgt cgaatcgcgt cgaggtgcgc
     3121 gtgcggcgcg acggcaacga gtacggcatg accttcgccg atggctacaa ggccagcgag
     3181 ctggaagtgc tgggcacggt gggcaagcgc aacagcggca ccagcgtgca gttctggccg
     3241 gacgccaagt acttcgatgc gccgaagttc tccatcagcc gcctcaagca tgtgctcaag
     3301 gccaaggcgg tgctctgtcc ggggctgacc gtgagcttcg aggacaagac cagcggcgag
     3361 aaggtcgagt ggttctacga ggacggtctg cgctcctacc tgagcgatgc ggtcagcgaa
     3421 ttcgcccgcc tgccggagga gccgttctgc ggcagcctgg ccggcagcag ggaagcggtc
     3481 gactgggcgc tgctgtggct gccggaaggc ggcgagagcg tgcaggaaag ctacgtcaac
     3541 ctgatcccca ccgcccaggg cggcacccac gtcaacggcc tgcgccaggg cctgctggac
     3601 gccatgcgcg agttctgcga gttccgcagc ctgctgccgc gcggggtcaa gctggcgccg
     3661 gaagacgtct gggagcgcat cgccttcgtg ctgtcgatga agatgcagga gccgcagttc
     3721 tccgggcaga ccaaggagcg tctgtcctcg cgtgaggcgg cggccttcgt ctcgggggtg
     3781 gtcaaggacg ccttcagcct gtggctcaac gagcatcccg agctgggcct ggcgctggcg
     3841 gaattggcga tcagcaacgc cgggcgccgc ctcaaggcgg gcaagaaggt cgagcgcaag
     3901 aagatcaccc agggcccggc gctgcccggc aagctggccg actgcgccgg ccaggacccg
     3961 atgcgcgccg agctgttcct ggtcgagggc gactcggccg gcggcagcgc caagcaggcg
     4021 cgcgacaagg agttccaggc catcctgccg ctgcgcggca agatcctcaa cacctgggag
     4081 gtggacggcg gcgaggtgct ggccagccag gaagtgcaca acatcgcggt ggccgtgggc
     4141 ctagatccgg gctccagcga tctgtcccag ctgcgctacg gcaagatctg catcctcgcc
     4201 gacgccgact ccgacggcct gcacatcgcc accctgctgt gcgcgctgtt cgtccagcat
     4261 ttccggccac tggtggaagc cggccatgtc tacgtcgcca tgccgccgct gtaccgcatc
     4321 gacctgggca aggaggtctg gtacgccctc gacgaggccg agcgcgacgg catcctcgag
     4381 cgtctgctcg ccgagaagcg ccgcggcaag ccccaggtca cccgcttcaa gggcctcggc
     4441 gagatgaatc cgccgcagct gcgcgagacc accatggacc ccaacacccg ccgtctggtg
     4501 cagctgaccc tggaggatgc ccagggcacc ctggagatca tggacatgct gctggccaag
     4561 aagcgcgccg gcgaccgcaa gaactggctg gagagcaagg gcaacctggc cgaggtgctg
     4621 ctttgaggca gcgctggcga ccgctcggcc gtctgctcgc cgcgctgcta ctcaccggca
     4681 gcgccctggc gcgcgccgag ccgctggagg agctggcgtt gcaggccgcc ctgccgg
//
LOCUS       DATCUN010000151         7895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_44189, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000151 DATCUN010000000
VERSION     DATCUN010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44189"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VIO83_06085"
     CDS             complement(<1..387)
                     /locus_tag="VIO83_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8330877.1"
                     /translation="MPHILIVEDETIIRSALRRLLERNQYQVSEAGSVQEARERYSIP
                     GFDLVISDLRLPGAPGTELIGLAAGTPVLIMTSYASLRSAVDSMKLGAIDYIAKPFDH
                     DEMLQAVARILRERQQARSAAAADTPA"
     gene            complement(543..3497)
                     /locus_tag="VIO83_06090"
     CDS             complement(543..3497)
                     /locus_tag="VIO83_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8330878.1"
                     /translation="MPTSFNLTHLLLISAAYLLVLFGIAWLTERGVMPRALVRHPLIY
                     TLSLGVYASAWAFYGAVGLAYQYGYGFLAIYLGLSGAFLLAPVLLYPLLRLTRTYQLS
                     SLADLFAFRFRSTWAGTLTTVFMLIAVLPLLALQIQSIADSVAIITREQPHAYVALIF
                     CALITLFTILFGARQIATRERHEGLVVAIAFESLIKLCAMTAVGLYALYGVFGGPAEL
                     EQWLLQNQTALSTLHTPLQEGPWRTLLLVFFAAAITMPHMFHMAFTENLNPRALVTAS
                     WGLPLFLLLMSLAVPPILWSGLKLGVATSPQYFTLGLGMQVGNPWLTLAAFIGGLSAA
                     SGLVIIVTLALSGMVLNHVVLPLYQPPAEGNIYRWLRWTRRGLIAAIIAASYGFYLLI
                     GAGQDLSHLGTVSFVATLQFLPGALSVLYWPTANRLGFISGLLAGASVWVLTMLLPLI
                     GNLPGFYLPLLDMVYVLDDTSWHIAAIASLATNVLVFTLVSLFSQPTSEEQSAAEACA
                     VDNVRRPQRRELVAVSPQEFAAELAKPLGARTAQREVEQALHDLQLPFDEQRPYALRR
                     LRDRIEANLSGLLGPSVAQDLVENFLPYKAGDETYVTEDIHFIESRLEDYQSRLTGLA
                     AELDALRRYHRQTLQELPMGVCSLAQDQEILMWNRAMEMLTGIEAQLVVGSRLGTLPE
                     PWRGLLSTFIDAPDEHLHKQRLALDGQTRWLNLHKAAIDEPLAPGSSGLVLLVEDLTE
                     TQLLEDRLIHSERLASIGRLAAGVAHEIGNPVTGIACLAQNLREERESDAEISEASQQ
                     IIEQTKRITRIVQSLMSFAHAGGLQQAREPVCLAEVTREAISLLSLNRDYDVQYFNLC
                     DPQHWVEGDPQRLAQVLINLLSNARDASPPGGAIRVRSEAGEHTVELMVEDEGSGIPK
                     SIVDRLFEPFFTTKDPGKGTGLGLALVYSIIEEHYGEISIDSPADPKHQRGTRIRITL
                     PRHGGPTAAENP"
     gene            complement(3481..3657)
                     /locus_tag="VIO83_06095"
     CDS             complement(3481..3657)
                     /locus_tag="VIO83_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330879.1"
                     /translation="MYIYRLVLLLVVGIYLFSPAIMDWWIDPNGAWYRPYLLWLILII
                     VTFILQSQRDADEL"
     gene            complement(3738..4622)
                     /gene="gluQRS"
                     /locus_tag="VIO83_06100"
     CDS             complement(3738..4622)
                     /gene="gluQRS"
                     /locus_tag="VIO83_06100"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEY8330880.1"
                     /translation="MNAPAYTGRFAPTPSGYLHFGSLVAALASYLDARAAGGRWLLRM
                     EDLDPPREVPGAQDAILHTLETYGFEWDGAVERQSTRHGAYAAALDGLLARGLAYACT
                     CSRKQLEGQPIYPGTCRNAGHSAANAAIRLRVPDAEYRFIDRVQGAFAQNLAHEVGDF
                     VIRRRDGLYAYQLAVVLDDAWQGVSDIVRGADLLDSTPRQLYLQELLGLPQPRYLHIP
                     LLLQPDGHKLGKSYRSPPLPPDQAPALLTRALRALGQQPPAELEQGNAARVLAWGIRH
                     WNPAAIARQAGLAEAQLR"
     gene            complement(4667..5098)
                     /gene="dksA"
                     /locus_tag="VIO83_06105"
     CDS             complement(4667..5098)
                     /gene="dksA"
                     /locus_tag="VIO83_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEY8330881.1"
                     /translation="MPTKTQDKIRAFEPYVETKGEEYMGDKMRAHFTAILNGWKKQLM
                     EEVDRTVHHMQDEAANFPDPADRASQEEEFSLELRTRDRERKLIKKIDETLQLIEDND
                     YGWCDSCGVEIGIRRLEARPTATLCIDCKTLAEIKEKQLGS"
     gene            5294..6475
                     /locus_tag="VIO83_06110"
     CDS             5294..6475
                     /locus_tag="VIO83_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY8330882.1"
                     /translation="MALSFSARSRAIQPFHVMALLARANELQAAGHDVIHLEVGEPDF
                     STAAPIVRAGQAALAAGHTRYTAARGLPALREALAGFYAQRYAVSIDPERILITPGGS
                     GALLLASALLVDPGKHWLLADPGYPCNRHFLRLVEGDAQLVPVGPQSNYQLTAELVAR
                     HWNADSVGALVASPANPTGTVLERGELAALSAALKARGGHLVVDEIYHGLTYGLDAPS
                     VLEVDDDAFVLNSFSKYFGMTGWRLGWLVAPPAAVAELEKLAQNLYISAPSMAQYAAL
                     ACFEPETLAILEQRRAEFARRRDYLLPALRALGFKIAVEPQGAFYLYADISAFGGDAQ
                     AFCRHFLETEHVAITPGLDFGHHLAGQHVRFAYTQSLPRLEQAVARIARGLQSWHPDA
                     V"
     gene            6465..7172
                     /gene="sfsA"
                     /locus_tag="VIO83_06115"
     CDS             6465..7172
                     /gene="sfsA"
                     /locus_tag="VIO83_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEY8330883.1"
                     /translation="MQFEPPLEEGRLLRRYKRFLADIETVQGETLTLHCPNTGSMHNC
                     MSEGGRVWFSRSSDPKRKLPGTWELSETPQGRLACVNTARANALVEEALRDGVIAELA
                     GFTQLRREVRYGEENSRVDFRLDYAHGPAFVEVKSVTLGFHGTPVAAFPDARTARGSK
                     HLRELASLARQGVRAVQLYCVNLSAIEAVRPAAEVDPQYAQALQAAVTAGVEVLAYGA
                     ELSPAGIRLTRRLEVIL"
     gene            complement(7218..7535)
                     /locus_tag="VIO83_06120"
     CDS             complement(7218..7535)
                     /locus_tag="VIO83_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY8330884.1"
                     /translation="MQQLCNSDELAEGASRGFGDGSRSLLLVRREGQVFAYRNRCPHR
                     GIPLEWQADQFLDVSASLIQCATHGALFLIESGECVAGPCAGERLEALPCREQDGSIW
                     VEL"
     gene            7725..>7895
                     /locus_tag="VIO83_06125"
     CDS             7725..>7895
                     /locus_tag="VIO83_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY8330885.1"
                     /translation="MLAFWLALALGPVSLSLGDSLRALLRLLGLPLDGADLAQAELIV
                     GQIRLPRALLGLA"
BASE COUNT         1169 a         2505 c         2927 g         1294 t
ORIGIN      
        1 ggctggggtg tcggccgctg cggcgctgcg cgcctgctgg cgttcgcgca ggatgcgcgc
       61 caccgcctgg agcatttcgt cgtggtcgaa gggcttggcg atgtagtcga tggcgccgag
      121 cttcatcgag tcgaccgccg aacgcaggct ggcgtagctg gtcatgatca gcaccggagt
      181 gccggccgcc aggccgatca gctcggtacc cggtgcgcca ggcaggcgca ggtcgctgat
      241 caccaggtcg aagccgggga tgctgtaacg ctcgcgcgct tcctgtaccg agccggcctc
      301 gctgacctgg tactggttgc gctccagcag gcgccgcagc gctgagcgaa tgatggtttc
      361 gtcttcgacg atgagaatat gtggcatgtc tgttcagctc tcgcgacgtg ctcggaagcg
      421 gctggaaact gtctgcgccg gtctctgcgg acggcttcgc gggggcgcct ggaaggggcg
      481 gtggaccgcg cgccgcggcg tgttgtcacg ctgtggcgct gcggttgcag tgtccatggg
      541 cttcacgggt tttcggcagc cgtcgggcca ccatggcgcg gcagggtgat ccggatgcgc
      601 gtgccgcgct ggtgcttcgg gtctgccggg ctgtcgatgc ttatttctcc ataatgctcc
      661 tcgatgatcg aatagaccag tgcgaggccc aggccggtcc ctttgccggg gtctttggtg
      721 gtgaagaagg gttcgaacag gcggtcgacg atcgacttgg gaatgccgct gccctcgtcc
      781 tcgaccatca gctcgacggt gtgctcgccg gcctccgagc gcacgcggat ggcgccgccg
      841 ggcggcgagg cgtcacgggc attggacagc aggttgatca gcacttgggc caggcgctgc
      901 gggtcgccct cgacccagtg ctgcggatcg cacaggttga agtactgcac gtcgtagtca
      961 cggttgagcg acagcaggct gatcgcctcg cgcgtcacct ccgccaggca caccggctcg
     1021 cgggcctgct gcaggccgcc ggcgtgggcg aagctcatca gcgactggac gatgcgggtg
     1081 atgcgcttgg tctgctcgat gatctgctgg ctggcctcgc tgatttcggc atcgctttcg
     1141 cgctcctcgc gcaggttctg cgccaggcag gcgataccgg tgaccgggtt gccgatctcg
     1201 tgggccaccc cggcggccag gcggccgatc gaggccaggc gctcggaatg gatcaggcgg
     1261 tcttccagca gctgggtctc ggtgaggtcc tcgaccagca gcaccaggcc gctgctgcca
     1321 ggtgccagcg gctcgtcgat ggcggccttg tgcaggttca gccagcgggt ctggccgtcg
     1381 agggccaggc gctgcttgtg caggtgctcg tcgggcgcgt cgatgaaggt gctcagcagc
     1441 ccgcgccagg gctcgggcag ggtgccgagc cgcgagccga ccaccagctg cgcctcgatg
     1501 ccggtgagca tctccatggc ccggttccac atgaggattt cctggtcctg ggccagcgag
     1561 cagacgccca tcggcagctc ctgcagggtc tgccggtggt agcggcgcag ggcgtcgagc
     1621 tcggcggcga ggccggtgag gcgcgactgg tagtcttcca ggcggctctc gatgaagtgg
     1681 atgtcctcgg tgacgtaagt ctcgtcgccg gccttgtagg gcaggaagtt ctcgacgagg
     1741 tcctgggcca cgctcgggcc cagcaggccg gagaggttgg cctcgatgcg atcgcgcagg
     1801 cggcgcaggg cgtaggggcg ctgctcgtcg aaaggcagct gcaggtcgtg caaggcctgt
     1861 tccacctcgc gctgggcggt acgtgcgccg agcggcttgg ccagttcggc ggcgaactcc
     1921 tgcggcgaaa cggcgaccag ttcgcggcgc tgcgggcggc gcacgttgtc caccgcgcag
     1981 gcctcggcgg cgctctgttc ctcgctggtc ggctggctga acagcgagac cagggtgaac
     2041 accagcacgt tggtcgccaa cgaagcgatg gctgcgatgt gccagctggt gtcgtcgagc
     2101 acgtagacca tgtcgagcag cggcaggtag aagccgggca ggttgccgat cagcggcagc
     2161 agcatggtca ggacccacac gctggcgccg gccagcaggc cgctgatgaa gccgaggcga
     2221 ttggccgtcg gccagtacag caccgacagg gcgccgggga ggaactgcag ggtggcgacg
     2281 aaggatacgg tacccaggtg cgaaaggtcc tggcccgccc cgatcagcag gtagaagccg
     2341 tagctggcgg cgatgatcgc ggcgatcagg ccgcggcggg tccacctcag ccagcggtag
     2401 atgttgccct cggccggtgg ctggtacagc ggcagcacga catggttgag caccatgccc
     2461 gacagcgcga gggtgacaat gatcaccagg ccactggctg cggacaggcc gccgatgaag
     2521 gcggcgaggg tcagccaggg gttgcccacc tgcatgccca ggccgagggt gaaatattgc
     2581 gggctggtgg ccacgccgag cttgaggccg ctccacagga tcgggggtac cgccaggctc
     2641 atcagcagca ggaacagcgg caggccccag ctggcggtga ccagcgcccg cgggttgagg
     2701 ttctcggtga aggccatgtg gaacatgtgc ggcatggtga tggcggccgc gaagaacacc
     2761 agcagcaggg tgcgccacgg accttcctgc agtggcgtat gcagggtgct gagggcggtc
     2821 tggttctgca gcagccactg ttcgagctcg gcgggcccgc cgaacacgcc gtacagcgcg
     2881 tacaggccga cggcggtcat cgcgcacagt ttgatcagtg attcgaaggc gatggcgacc
     2941 accaacccct catggcgctc gcgggtggcg atctgccgtg caccgaagag gatggtgaac
     3001 agggtgatca gggcgcagaa gatcagcgcc acgtaggcgt ggggctgctc gcgggtgatg
     3061 atggcgacgc tgtcggcgat cgactggatc tgcagggcca gcagcggcag caccgcgatc
     3121 agcatgaaga cagtggtcag ggtgccggcc caggtgctgc gaaagcggaa ggcgaacagg
     3181 tcggccagcg acgacagctg gtaggtgcgg gtcaggcgca gcagcggata gagcagcacc
     3241 ggtgccagca ggaaggcacc cgaaagcccc aggtagatgg cgagaaagcc gtagccatac
     3301 tggtaggcca ggccgaccgc gccatagaag gcccaggcgc tggcgtacac acccagtgac
     3361 agggtgtaga tcagcgggtg gcgcaccagc gcgcgcggca tcacgccgcg ctcggtcagc
     3421 caggcgatgc cgaacaggac cagcaggtag gcggcgctga tcagcagcag gtgggtgagg
     3481 ttaaagctcg tcggcatcgc gctgactctg gaggatgaag gtgacgatga tcaggatcag
     3541 ccacagcagg tacggtcggt accaggcgcc gttcgggtcg atccaccagt ccatgatggc
     3601 cggggaaaac aggtagatcc ccaccaccag gagcaggacc agccggtaga tatacatggg
     3661 atacctcgtc gtgggatgcc cggatggtaa cggaggcgcg cgcgaagcag aagccggtaa
     3721 cagtcggggt tgtactgtta ccgcagctgt gcctcggcga ggcccgcctg gcgggcaatg
     3781 gcggcgggat tccagtggcg gatcccccag gccagcacgc gggcggcgtt accctgctcc
     3841 agttcggccg gcggctgctg gccgagggcg cgcagggcgc gggtcagcag ggccggggcc
     3901 tgatccggcg gcagcggcgg cgagcggtag gacttgccga gcttgtggcc gtccggctgc
     3961 agcagcaggg ggatgtgcag gtagcgcggc tgcggcaggc cgagcagctc ctgcaggtac
     4021 agctggcgcg gggtggagtc gagcaggtcg gcgccgcgca cgatgtcgct gaccccctgc
     4081 caggcgtcgt cgagcaccac cgccagctgg taggcgtaca ggccgtcgcg gcggcggatg
     4141 acgaagtcgc cgacctcgtg ggcgaggttc tgggcgaagg cgccctgcac ccggtcgatg
     4201 aagcggtact cggcatccgg tacgcgcagg cggatggcgg cgttggccgc gctgtggccg
     4261 gcattgcggc aggtgccggg gtagatcggc tggccttcga gctgtttgcg cgagcaggtg
     4321 caggcgtagg ccaggccgcg cgccagcagc ccgtcgagcg cggcggcata ggcgccatgg
     4381 cgggtgcttt gccgctcgac cgcgccgtcc cactcgaagc cataggtttc cagggtgtgg
     4441 aggatggcat cctgggcgcc ggggacctcg cgcggcgggt cgaggtcttc catgcgcagc
     4501 agccagcggc cgccggcggc gcgggcgtcg agataggagg cgagggcggc gaccagcgag
     4561 ccgaagtgca ggtagccgct gggcgtgggg gcgaaacggc cggtgtaggc gggggcgttc
     4621 atgggctgca gaaacagcgc ggggcgccgt ggcgccccgt tgcgggtcag gaacccagct
     4681 gcttttcctt gatctcggcg agggtcttgc agtcgatgca cagggtggcg gtcggccgcg
     4741 cctcgaggcg gcggatgccg atctcgacgc cgcaggagtc gcaccagccg tagtcgttgt
     4801 cctcgatcag ctgcagggtc tcgtcgatct tcttgatcag cttgcgctcg cggtcgcggg
     4861 tgcgcagttc caggctgaac tcctcttcct ggctggcgcg gtcggccgga tcggggaagt
     4921 tggcggcctc gtcctgcatg tggtgcacgg tgcggtcgac ttcctccatc agctgcttct
     4981 tccagccatt gaggatggcc gtgaagtggg cgcgcatctt gtcgcccatg tactcctcgc
     5041 ccttggtttc tacatagggc tcgaaggcgc ggattttgtc ttgggtcttg gtcggcatgg
     5101 tatgtccgcc tctcacgttc gctaatcctg tgcgcaggtt gtgtccatca gcggcatttt
     5161 agccggccct gcgcttgcaa gcgggcgaac ctaccagaat cgcggggcgg aagctactcc
     5221 ggtgtacacg gtgcctgccg cgtcgtgtga cagttggcta gaatcggcgc ttgtccgatc
     5281 cttggagggc catatggctc tgtccttcag tgcacgcagt cgcgccatcc agccctttca
     5341 cgtcatggcc ctgctggccc gcgccaacga gttgcaggcg gccggccacg acgtcattca
     5401 cctggaggtc ggcgagccgg acttctccac cgcggctccg atcgtgcgcg ccggccaggc
     5461 ggcgctggcc gccggacata cccgctacac cgcggcgcgc ggcctgccgg cgctgcgcga
     5521 ggcgctggcg ggcttctatg cccagcgcta cgcagtgagc atagaccccg agcgcatcct
     5581 catcaccccc ggcggcagcg gcgcgctgct gctggccagc gccctgctgg tcgatccggg
     5641 caagcactgg ctgctggccg atcccggtta tccgtgcaat cgccacttcc tgcgcctggt
     5701 tgagggcgat gcgcagctgg tgccggtggg gccgcagtcg aattatcagc tgaccgcgga
     5761 gctggtcgcg cgccactgga acgcagacag cgtcggcgcg ctggtcgcct cgccggccaa
     5821 ccccaccggc accgtgctcg agcgtggcga actggccgct ctgtccgccg cgctcaaggc
     5881 gcgcggcggc catctggtgg tggacgagat ctaccacggg ctgacctacg gcctggacgc
     5941 ccccagcgtg ctggaagtgg acgatgacgc ctttgtcctc aacagtttct ccaagtactt
     6001 cggcatgacc ggctggcgcc ttggctggct ggtggcaccg ccggccgcgg tggcggagct
     6061 ggagaagctg gcacagaacc tctacatcag cgcgccgagc atggcgcagt acgccgcgct
     6121 ggcctgtttc gagccggaga ccctggcgat cctcgagcag cgccgcgccg agttcgcccg
     6181 ccgtcgcgac tacctgctgc cggccctgcg ggcgctgggc ttcaagatcg ccgtcgagcc
     6241 gcagggcgcc ttctacctgt atgccgacat ctcggcgttc ggtggcgacg cccaggcgtt
     6301 ctgccggcat ttcctggaaa ccgagcatgt ggccatcacg ccgggactgg acttcggtca
     6361 ccacctggcc gggcagcacg tgcgcttcgc ctatacccag agcctgccgc gcctggagca
     6421 ggcggtcgcg cgcatcgccc gcggtctgca gagctggcat cccgatgcag tttgagccgc
     6481 cgctggagga aggtcgcctg ctgcgccgct acaagcgctt tctcgccgat atcgagaccg
     6541 tgcagggcga gacgctgacc ctccactgcc cgaacaccgg ctcgatgcac aactgcatga
     6601 gcgagggcgg ccgcgtctgg ttcagtcgct ccagcgatcc gaagcgcaag ctgccgggga
     6661 cctgggaact gagcgagacg ccgcaggggc ggctggcctg cgtcaacacc gcgcgggcca
     6721 acgcgctggt ggaggaagcg ctgcgtgacg gggtgatcgc cgagctggcg gggttcacgc
     6781 agctcaggcg cgaggtgcgc tatggcgagg aaaacagccg ggtggatttt cgcctggact
     6841 atgcgcacgg cccggcgttc gtcgaggtca agagcgtgac gctcggcttc cacggcacgc
     6901 cggtggcggc cttccccgat gcgcgcaccg cgcgcggcag caagcatttg cgcgagctgg
     6961 ccagcctggc gcgccagggc gtgcgcgccg tgcagctgta ttgcgtgaac ctctcggcca
     7021 tcgaggcggt acgcccggcg gcggaggtcg atccgcagta cgcgcaggcg ctgcaggcgg
     7081 cggtgacggc gggggtggag gtgctggcct atggcgccga actgtcgccc gcgggcatcc
     7141 gcttgacccg gcggctggag gtgatcctct agggtgctgc aggcgcaccc ggcgtgcggc
     7201 tggcgtcagc ggcgtgccta cagctcgacc cagatgctgc cgtcctgttc gcggcagggc
     7261 agggcttcga gacgttcccc ggcgcagggg ccggccacgc actcgccgga ctcgatgagg
     7321 aacagcgcgc cgtgggtggc gcactggatc aggctggcgc tgacgtcgag aaactggtcg
     7381 gcctgccact ccagcgggat gccgcggtgc gggcagcggt tgcggtaggc gaacacctgc
     7441 ccttcgcggc ggaccagcag caggctgcgg ctgccgtcac cgaagccgcg gctggcgcct
     7501 tcggccagtt cgtcgctgtt gcacagttgc tgcatggtgg tctccttggg ctgcgccgat
     7561 tatccgcagt ggccggcagt tctgccagtt cgcgggccgc cgtcgtgggg caggagcgcg
     7621 gcggcctgtt atgctgctgc cccgtttccc gagcaaggca acccgtcgcg atgaaccgcc
     7681 cggcctgcgg tcccttcctg cttcccgtgc tggggctggc gctgctgctg gccttctggc
     7741 tggcgctggc gctcggtccg gtgagcctgt cgctgggcga cagcctgcgc gcgctgctgc
     7801 gcctgctcgg cctgccgctg gacggggccg atctggcaca ggccgagctg atcgtcggcc
     7861 agatccgcct gccgcgcgca ttgctcggcc tcgcc
//
LOCUS       DATCUN010000152        10507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_44262, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000152 DATCUN010000000
VERSION     DATCUN010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10507
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44262"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VIO83_06130"
     CDS             complement(<1..199)
                     /locus_tag="VIO83_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8330886.1"
                     /translation="MSAHDLKRGIPLHLHGVAKAFGERSVLHDIDLHIPAGQFVAVVG
                     RSGCGKSTLLRLLAGLDEASGG"
     gene            complement(186..989)
                     /gene="ssuC"
                     /locus_tag="VIO83_06135"
     CDS             complement(186..989)
                     /gene="ssuC"
                     /locus_tag="VIO83_06135"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HEY8330887.1"
                     /translation="MSKSNTERLLLRLAPWALPVALLAIWQITVVTGLLSTRILPAPS
                     AVLSAGWALLNSGELWQHLAISGWRAGIGFAIGGSIGLLLGFVTGLSQWGERLLDSSV
                     QMIRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYLNTYHGIRSVDAGLVEMAR
                     SYGLSGLPLFRQVILPGALPSILVGVRFALGFMWLTLIVAETISANAGIGYLAMNARE
                     FLQTDVVVLAILLYAVLGKLADVAARSLERAWLRWHPAYQQGGIQNERP"
     gene            complement(1092..2240)
                     /gene="ssuD"
                     /locus_tag="VIO83_06140"
     CDS             complement(1092..2240)
                     /gene="ssuD"
                     /locus_tag="VIO83_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HEY8330888.1"
                     /translation="MSLNIFWFLPTHGDGHYLGTAEGARAVDHGYLTQVAQAADRLGF
                     GGVLIPTGRSCEDSWLVAASLIPATQRLKFLVALRPGIISPTVAARQAATLDRLSNGR
                     ALFNLVTGGDPDELAADGLNLSHAERYEAAVEFTQVWRKVLEGETVDLDGKHIQVKGA
                     KLLYPPIQQPRPPLYFGGSSEAAHELAAEQVELYLTWGEPPQAVAEKIADVRARAARH
                     GRTVKFGIRLHVIVRETVDEAWAAAERLISHLDDDTIAKAQASLARFDSVGQQRMAAL
                     HGGSKDNLEVAPNLWAGVGLVRGGAGTALVGDGPTVAARVKEYADLGIDTFVFSGYPH
                     LEESYRVAELLFPHLDIAQPERPQSRGYVSPFGEMISSDILPKAAAQS"
     gene            complement(2349..3311)
                     /locus_tag="VIO83_06145"
     CDS             complement(2349..3311)
                     /locus_tag="VIO83_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8330889.1"
                     /translation="MRTPYLRRGLVALLAAAISSAALAADDAHTLRIGYQKYGTLVLL
                     KAKGSLEQRLAAQGVKVQWTEFPGGPQLLEGLNVGSIDFGVTGETPPVFAQAAGADLR
                     YVAYEPPAPTGEAILVPKGSSIKSVAELKGKKVALNKGSNVHYLLVRALEDAGLSIKD
                     ITPVYLPPADARAAFERGSVDAWVIWDPFQAAAETQMQARTLRDGSGLVDNHQFYLAS
                     GSYAQQHPQVVGALIEEIRAVGEDSKADPAKVTAQVAPLIGLSPEITHKAVTRQGYGA
                     QPLTPAVVEAQQKIADTFADLKLIPKRLSIKDVVWMPPAKVAQH"
     gene            complement(3462..4049)
                     /gene="ssuE"
                     /locus_tag="VIO83_06150"
     CDS             complement(3462..4049)
                     /gene="ssuE"
                     /locus_tag="VIO83_06150"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019694161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEY8330890.1"
                     /translation="MLVVSLSGSPSQQSRSAGLLEWSRRWLSVRGVEVVSFGVRDFAA
                     EDLLYGRFDSPQVIELAERVGAADGLLVATPVYKAAYSGALKTLLDLLPERALAHSVV
                     LPLATAGSPAHLLALDYALKPVLAALKAEEVLQGVFADDRQTRYEEGRLVLSEELEQR
                     LEAALEQFYQALARRPRHLEPGQLEQQLLGARWSI"
     gene            complement(4133..4771)
                     /locus_tag="VIO83_06155"
     CDS             complement(4133..4771)
                     /locus_tag="VIO83_06155"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY8330891.1"
                     /translation="MSIRLGDIAPDFEQDSSAGRIRFHAWLGDSWGILFSHPADFTPV
                     CTTELGFTAKLKDQFAQRGVKAIALSVDPVESHLKWIEDINETQDTQVNFPILADADR
                     KVSDLYDLIHPNASDTLTVRSLFIIDPAKKVRLIITYPASTGRNFHEILRVIDSLQLT
                     DSHKVATPANWQDGDEVVIVPSLKDEAELQQRFPKGYRAVKPYLRLTPQPNR"
     gene            5088..8027
                     /locus_tag="VIO83_06160"
     CDS             5088..8027
                     /locus_tag="VIO83_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8330892.1"
                     /translation="MDRGTERVRFGLQQVHPQHRDSLGQPLAVLVILLAGVLLTTQFA
                     AAIPPVAGYLAWHMLLETTAIAVAVLVFAVGWSTHGLHPQRNILLLACGFLGVAILDF
                     SHMLSFLGMPDYVTPSGVEKAIHFWLVARYLAALALLLVAWLPWPPVGAEQRPRSERR
                     RFVPLLGVLLGVALMHWLFLYRPQWLPATFVAGQGLTPFKLWAEYGVIGVHLLTVAVL
                     LARLRQASGCNVAQLLAAVLAMIFSELFFTRYASATDVFNLMGHVYKVLAYLLLYRAV
                     FVEMITAPYGALSDARRHSQAILEAIPDLLFELDCDGRYLQAHAPRNEQLAAGADALL
                     GRTVYEVLPLEAARTCMAALQEAQANGHASGQQIQLPLADGIHCFELSVSVMRQDHDR
                     APRFVMLSRDVTARTRDQATLRKLSQAVEQSASTIVITDLDARIEYANQAFTRATGYS
                     LEEAIGKNPKLLHSGKTPQSTYEDMWQHLTSGRAWRGEFVNQRKDGSEYTESVLISPV
                     FDDAGQPTHYLAIKEDITQRKLDEQRIERLAHFDALTDLPNRKLFASRCEQALGLSER
                     SHQPLAVLYMDLDHFKHINDSLGYRVGDAFLVEVAQRLKAALRDEDTLSRQGGDEFVF
                     VLPYTDAKGAAHAAERLKALLASPCEVGEHSLAINASIGIAVFPEDGRDFDTLSQRAD
                     IAMHRAKQEGRNGYRFFTAEMQRQSARILQLESALRQALDRHELHLCFQPQVSIDGQR
                     LVGAEALLRWTHPQLGVISPAEFIPVAEGSGQILQVGEWVLRSAARQMKHWLEQGYPP
                     TMTMAVNLSLAQFRHPGLVELVVDVLQETGLPAHCLELELTESVAMYHPAAAIEVVRR
                     LRTLGVLLSIDDFGTGYSSLSYLKQFEVHKLKIDQSFVQHINQDGHDQSIVRAILGMA
                     SSLGMLTIAEGVETAAQRDELERLGCDEVQGYLFSKPLPAAEFEAFVCQHLEQVGKCP
                     ALAATPA"
     gene            complement(8089..9435)
                     /locus_tag="VIO83_06165"
     CDS             complement(8089..9435)
                     /locus_tag="VIO83_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019407456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015802 - basic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family porin"
                     /protein_id="HEY8330893.1"
                     /translation="MNKSPLALAVFAATLASALSGAAQAAFLEDSKASLALRNFYYDR
                     NDKNTADNRNEASEWGQGFIFNYQSGFTEGTVGFGVDAVGLLGVRLDGGGDGDRTDQR
                     KPGQLFPTESNGKAVHEYSKAGLTAKVKLSKTEARLGTLQPKLPVVTFNDGRLLPQLF
                     EGGQIISNEIDGLTLTTGQLEHTKTRNSTDDIGLRIAGATAVNGIQADTNKFYFAGGD
                     YKLTKDLTAQYYYGNLEDFYDQHFLGLVHNWALPVGALKSDLRYFDSSSDGKNSSAAG
                     RAEGYRSSGYWNGGTNNPDNGEVDNQTWSALFTYTLGGHALSAGYQQVSGDSAFPFLN
                     QGDGSTAYLITDVQINKFLAAGERTWLAGYAYDFAKLGVPGLKASALYLSGDNIDAAG
                     SDKQEWERNLRLDYVLQEGTLKGLGFTWRNASLRGNTSTADQDENRLIVSYTLPLL"
     gene            complement(9589..9714)
                     /locus_tag="VIO83_06170"
     CDS             complement(9589..9714)
                     /locus_tag="VIO83_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330894.1"
                     /translation="MNNRACGAGTARRPIFRQPGLLIEAGLLARTSYSLIAFKNK"
     gene            10183..>10507
                     /locus_tag="VIO83_06175"
     CDS             10183..>10507
                     /locus_tag="VIO83_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8330895.1"
                     /translation="MIYRPLGKTGLQVSALALGTMTWGEQNTEAEAFEQIRRAQAAGI
                     TFIDTAEIYPVPPKAETAGATETIIGNYFQRHGGRSDWILASKAAAPGNGITHIRGGR
                     NHFDRA"
BASE COUNT         1646 a         3387 c         3663 g         1811 t
ORIGIN      
        1 cgccaccgct ggcctcgtcg aggccggcca gcaggcgcag cagggtgctc ttgccacagc
       61 cgctgcgacc gaccacggcg acgaactggc cggcggggat gtgcaggtcg atgtcgtgca
      121 gcacgctgcg ctcgccgaag gccttggcca cgccatgcag gtgcagcggg atgccgcgtt
      181 tgaggtcatg ggcgctcatt ttggatacct ccttgctggt aggccgggtg ccagcgcagc
      241 caggcgcgct caaggctgcg ggcggcgaca tcggccagct tgccgagcac ggcgtagagc
      301 aggatcgcca gcaccaccac gtcggtctgc aggaactcgc gggcgttcat cgccaggtag
      361 ccgatgccgg cgttggccga gatggtttcg gcgacgatca gggtcagcca catgaaaccg
      421 agggcgaagc gcacgccgac caggatcgac ggcagggcgc cgggcaggat cacctggcgg
      481 aacaggggca agcccgacag gccgtagctg cgcgccattt ccaccaggcc ggcgtcgacg
      541 ctgcggatgc cgtggtaggt gttgagatag atggggaaca gggtgccgag ggcgaccagg
      601 aagatcttcg cgctctcgtc gatgccgaac cacaggatca ccagcgggat cagcgccagg
      661 tgcggcacgt tgcggatcat ctgcaccgag ctgtcgagca ggcgttcgcc ccattgcgac
      721 aggccggtga cgaagcccag cagcaggccg atgctgccgc cgatggcaaa gccgatgccg
      781 gcgcgccagc cactgatggc caggtgttgc cagagctcgc cgctgttcag cagcgcccag
      841 ccggcactca gcacggcgct gggcgcgggc aggatgcggg tggacagcag tccggtcacc
      901 accgtgatct gccagatggc cagcagcgcc acgggcagcg cccaaggggc caggcgcagg
      961 agaagtcttt cggtattgct cttgctcatg atgccaactc gtgtaggggc gaattcattc
     1021 gccttgggca cgccgtggtg ggcgaatgaa ttcgccccta cagcgtgctc cggcgagcgg
     1081 ggttcgcggc ctcagctctg cgccgccgcc ttgggcagga tgtcgctgga gatcatctcg
     1141 ccgaacgggc tgacgtagcc gcggctctgc gggcgctctg gctgggcgat gtccaggtgc
     1201 gggaacagca gctcggcgac ccgataggac tcctccaggt gcgggtagcc ggagaacacg
     1261 aaggtgtcga tgccgagatc cgcgtattcc ttgacccgtg ccgccacggt cgggccgtcg
     1321 ccgaccagcg cggtgccggc gccgccgcgc accaggccga ccccggccca caggttgggc
     1381 gccacttcca ggttgtcctt gctgccgcca tgcagggcgg ccatgcgctg ctggccgacc
     1441 gagtcgaagc gcgccagcga ggcctgcgcc ttggcgatgg tgtcgtcgtc caggtggctg
     1501 atcagccgct cggcggcggc ccaggcttca tcaacggtct cgcgcacgat cacgtgcagg
     1561 cggatgccga acttcaccgt gcggccgtgc ctggcggcgc gcgcgcgcac gtcggcgatc
     1621 ttctccgcca ccgcctgtgg cggctcgccc caggtcaggt acagctcgac ctgctcggcg
     1681 gccagctcgt gggccgcctc ggaagaacca ccgaagtaca gcggtgggcg cggctgctgg
     1741 atcggtgggt agagcagctt ggcgcccttg acctggatgt gcttgccgtc gaggtcgacg
     1801 gtctcgccct ccagcacctt gcgccagacc tgggtgaact ccacggccgc ctcgtagcgc
     1861 tcggcgtggc tgaggttgag gccgtcggcc gccagttcgt ccgggtcgcc gccggtgacc
     1921 aggttgaaca gcgcgcggcc gttggacagg cgatcgaggg tcgccgcctg gcgcgccgcc
     1981 acggtcggcg agatgatccc cgggcgcagg gcgacgagga atttcaggcg ctgggtggcc
     2041 gggatcagcg aggcggcgac cagccaggag tcctcgcagg aacgcccggt ggggatcagc
     2101 acgccgccga aaccgaggcg gtcggcagcc tgggcgacct gggtcaagta gccgtggtcg
     2161 acggcgcggg cgccctcggc ggtgcccagg tagtggccgt cgccgtgggt ggggagaaac
     2221 cagaagatgt tcagactcat cgcagtattc ctgacaatcg ggcgcgcgtg gggcttggct
     2281 catgcgcgcg atgattcatg ggagcggggc agtcgcgggc ggccgcttgg gcgcccccgc
     2341 gatgcgggtc agtgctgggc gaccttggcc ggcggcatcc agaccacgtc cttgatgctc
     2401 aggcgcttgg gaatcagctt gaggtcggcg aaggtgtcgg cgatcttctg ctgggcctcc
     2461 actacggccg gggtgagcgg ctgggcgccg tagccctggc gggtcaccgc cttgtgggtg
     2521 atttccgggc tcaggccgat cagcggggcg acctgggcgg tgaccttggc cggatcggcc
     2581 ttggagtctt cgccgacggc gcggatttcc tcgatcagcg cgccgaccac ctgcgggtgc
     2641 tgctgcgcat agctgccgct ggccagatag aactggtggt tgtcgaccag cccgctgccg
     2701 tcgcgcaggg tgcgcgcctg catctgcgtc tcggcggcgg cctggaaggg atcccagatc
     2761 acccaggcgt cgacgctgcc gcgctcgaag gcggcgcggg cgtcggccgg cggcaggtac
     2821 accggggtga tgtccttgat cgacaggccg gcatcttcca gcgcgcgcac cagcaggtag
     2881 tgcacgttgg agcccttgtt gagggcgacc ttcttgccct tcagctcggc taccgatttg
     2941 atcgacgagc ccttgggtac caggatcgcc tcgccggtcg gcgccggcgg ttcgtaggcc
     3001 acgtagcgca ggtcggcgcc ggcggcctgg gcgaacaccg gcggggtctc gccggtcacg
     3061 ccgaagtcga tcgaaccgac gttgaggcct tccagcagct gcgggccgcc ggggaactcg
     3121 gtccactgca ccttgacgcc ctgggcggcg aggcgctgtt ccagactgcc cttggccttg
     3181 agcaggacca gggtgccgta tttctggtag ccgatgcgca gggtgtgggc gtcatcggcg
     3241 gcgagggcgg cggaggagat cgctgcagcc agcagggcga ccagtcctcg acgcaggtag
     3301 ggtgtgcgca tggcgctctc cttaatatgc atgtcggttg tggtggatgc ctgctggccc
     3361 gttggcgggc gagtaagggc tttgcgctgc ctcgccggtg tgggcgaggg cttgaatcag
     3421 tgggctgcgc agatacgctc gcccgcagga tgagtcgtgg ttcagatgct ccagcgcgcg
     3481 ccgagcagct gctgctcgag ctggccgggt tcgagatgcc gcggccggcg cgccagcgcc
     3541 tggtagaact gttccagtgc ggcttccagt cgctgttcca gttcctcgct gaggaccagg
     3601 cggccctcct cgtagcgggt ctggcggtcg tcggcgaaca cgccctgcag cacttcctcg
     3661 gccttcagcg ccgccagcac cggcttcagc gcgtagtcca gcgccagcag gtgggccggg
     3721 ctgccggcgg tggccagcgg cagcaccacc gaatgggcca gggcgcgttc gggcagcagg
     3781 tcgagcaggg tcttgagcgc cccggagtag gccgccttgt acaccggcgt ggcgaccagc
     3841 aggccgtcgg cggcgcccac gcgctcggcc agctcgatca cctgcgggct gtcgaagcgc
     3901 ccgtagagca gatcctcggc ggcgaagtcg cgcacgccga agctgaccac ttccacgccg
     3961 cgcacgctca gccagcgacg cgaccactcc agcaggccgg cggagcgcga ctgctgggag
     4021 ggactgccgg aaagggatac caccagcatc tgcgtttcct tctgttgcga ataaaaaagg
     4081 ggacctgtcc tgccgttcag gcccccaacc ccaagtggtc aaacggtcgg catcagcggt
     4141 tcggttgcgg cgtcaggcgc aggtacggct tcaccgcgcg gtagcctttg ggaaagcgct
     4201 gctggagctc ggcctcgtcc ttgagcgacg gcacgatcac cacctcgtcg ccgtcctgcc
     4261 agttggccgg ggtggcgacc ttgtggctgt cggtcagctg cagcgaatcg atcacccgca
     4321 ggatctcgtg gaagttgcgc ccggtgctgg ccgggtaggt gatgatcagg cgtaccttct
     4381 tggccgggtc gatgatgaac agcgagcgca cggtgagggt gtcgctggcg ttcgggtgga
     4441 tcaggtcgta gaggtcggac accttgcggt cggcgtcggc gaggatcggg aagttgacct
     4501 gggtgtcctg ggtctcgttg atgtcctcga tccacttcag gtgcgactcc accgggtcca
     4561 ccgacagggc gatggccttg acgccacgct gggcgaattg atctttcagc ttggcggtaa
     4621 agcccagctc ggtggtgcac accggggtga agtcggccgg gtgggagaac aggatgcccc
     4681 agctgtcgcc gagccatgcg tggaaacgaa tgcgaccggc gctggaatcc tgttcgaagt
     4741 cgggggcgat atcgccaagg cggatgctca tcctgttgcg ctcctgcggt agggtgtgtg
     4801 ggggctacta tgcgcaggag cgtaagatat taaaaagaat aaataatgat ttgtttattc
     4861 ctttatttat ataagagagc gcggtgaacc accggtagcg gcgcggccga gcagatcttc
     4921 aggcgccatg aaatcgtcga ttctgcgccg tatggcgacc atggtcggcg cgagcgcggc
     4981 aatcattgac ggggggcggt caggcaggca cattccgcgt cacattggcg ccgtggaaga
     5041 ctgtcacact ggcgcctgcc ggattctccg gtaacgacag tcggttcatg gacagaggta
     5101 ccgagcgcgt gcgattcggc ttgcagcagg ttcatccgca acaccgggac tcactcggcc
     5161 agcccctggc ggtgctggtc atcctgctgg ccggcgtgct gctgaccacc cagttcgccg
     5221 cggcgatacc gccggtcgcc ggctacctgg cctggcacat gctgctggaa accaccgcca
     5281 tcgccgtggc cgtgctggtc ttcgccgtcg gctggagcac ccacggcctg cacccgcagc
     5341 gcaacatcct gctgctggcc tgcggctttc tcggcgtcgc catcctcgac ttttcccaca
     5401 tgctgtcgtt cctgggcatg cccgactacg tgacccccag cggcgtggaa aaggccatcc
     5461 acttctggct ggtggcccgc tacctggccg ccctggcgct gctgctggtc gcctggctgc
     5521 cctggccgcc ggtgggcgcc gagcagagac cgcgctccga gcgccgccgc ttcgtgcccc
     5581 tgctgggcgt tttgctcggc gtggccctga tgcattggct gttcctttat cggccacagt
     5641 ggctgccggc gaccttcgtc gccggccagg gactcacccc gttcaagctg tgggccgagt
     5701 acggcgtgat cggcgtccac ctgctcaccg tcgccgtgct gctcgcccgc ctgcgtcagg
     5761 ccagtggctg caacgtcgcg cagctgctcg ctgcggtgct ggcgatgatc ttcagcgagc
     5821 tgttcttcac ccgctatgcc tcggccaccg acgtgttcaa cctgatgggc cacgtctaca
     5881 aggtgctggc ctacctgctg ctgtatcgcg cggtgttcgt cgagatgatc accgccccct
     5941 atggcgcgct cagcgatgcc cgccggcact cccaggccat tctcgaggcg attcccgacc
     6001 tgctgttcga actcgattgc gacggccgct acctgcaggc gcacgcgccg cgtaacgaac
     6061 aactggcggc cggcgccgat gcgctgctcg gccggacggt gtacgaggtg ctgccgctcg
     6121 aggccgcgcg cacctgcatg gccgcgttgc aggaggcaca agccaacggc catgcatcag
     6181 gccagcagat ccagctcccg ctggccgacg gcatccactg cttcgagctg tcggtgtcgg
     6241 tcatgcgcca ggaccacgac cgcgcgccgc gcttcgtcat gctgtcgcgc gacgtcaccg
     6301 ctcgcacccg cgaccaggcc accctgcgca agctgagcca ggcggtggag cagagtgcga
     6361 gcaccatcgt catcaccgac ctcgatgcgc gcatcgaata cgccaaccag gccttcaccc
     6421 gcgcgaccgg ctacagcctg gaggaggcca ttggtaaaaa ccccaagttg ctgcattccg
     6481 gcaagacccc gcagagcacc tacgaggaca tgtggcagca cctcaccagt ggccgggcct
     6541 ggcgtggcga gttcgtcaac cagcgcaagg acggcagcga gtacaccgag tcggtgctga
     6601 tctcgccggt cttcgacgac gccggccagc ccacccatta cctggcgatc aaggaagaca
     6661 tcacccagcg caagctggac gagcagcgca tcgagcgcct ggcgcacttc gacgcactga
     6721 ccgacctgcc caaccgcaag ctgttcgcct cccgctgcga gcaggcgctg ggcctctccg
     6781 aacgcagcca ccagccgctg gcggtgctgt acatggatct ggaccacttc aagcacatca
     6841 acgactcgct gggttaccgc gtcggcgacg ccttcctggt cgaagtcgcc cagcgcctga
     6901 aggccgccct gcgcgacgag gacaccctgt cgcgccaggg cggcgacgag ttcgtcttcg
     6961 tcctgcccta taccgatgcc aagggcgcgg cgcatgccgc cgagcggctg aaggcgctgc
     7021 tggccagtcc ctgcgaggtg ggcgaacaca gtctggcgat caatgcctcg atcggcatcg
     7081 cggtattccc cgaggatggc cgcgacttcg acaccctgtc gcagcgcgcc gatatcgcca
     7141 tgcaccgggc caaacaggag gggcgcaacg gctatcgctt cttcaccgcg gaaatgcagc
     7201 gccagtcggc gcgcatcctg cagctggaga gtgcgctgcg ccaggcgctg gaccggcacg
     7261 agctgcacct gtgcttccag ccccaggtga gcatcgacgg ccagcggctg gtcggcgccg
     7321 aagccctgct gcgctggacc cacccgcagc tgggggtgat cagtccggcc gagttcattc
     7381 cggtcgccga gggcagcggc cagatcctgc aggtcggcga atgggtgctg cgcagcgcgg
     7441 cgcggcagat gaagcactgg ctggagcagg gctacccgcc gaccatgacc atggcggtga
     7501 acctgtcgct ggcgcagttc cgccatcccg ggctggtcga gctggtcgtc gacgtcctgc
     7561 aggaaaccgg cctgccggcc cactgcctgg agctggagct gaccgagagc gtggccatgt
     7621 accatccggc ggcggccatc gaggtggtgc ggcgcctgcg cacgctgggc gtattactgt
     7681 cgatcgacga tttcggcacc ggttattcgt cgcttagcta cctcaagcag ttcgaggtgc
     7741 acaagctgaa gatcgaccag tccttcgtgc agcacatcaa ccaggacggt cacgaccagt
     7801 ccatcgtgcg ggcgattctg ggcatggcga gcagcctggg catgctgacc atcgccgaag
     7861 gcgtggagac ggcggcgcaa cgggacgagc tcgaacgcct gggttgcgac gaggtgcagg
     7921 gctacctgtt cagcaagccg ctgccggcgg cggagttcga ggccttcgtg tgtcaacacc
     7981 tggagcaggt gggcaagtgc cccgcgctgg ccgctacccc ggcctgagcg cggcactgcg
     8041 cggcgggcaa gccggcagca cggcgcgggc cgggctgccg gtggagactt acagcagcgg
     8101 cagggtgtag ctgacgatca ggcggttctc gtcctggtcg gcagtcgagg tgttgccgcg
     8161 cagcgaggca ttgcgccagg tgaagcccag gcccttgagg gtgccttcct gtagcacgta
     8221 gtccaggcgc aggttgcgct cccattcctg cttgtcgctg ccggcggcat cgatgttgtc
     8281 gccgctcagg tacagcgcgc tggccttcag gcccggcacg ccgagcttgg cgaagtcgta
     8341 ggcatagccg gccagccagg tgcgttcgcc ggcggcgagg aacttgttga tctgcacgtc
     8401 ggtgatcagg tacgcggtgg agccgtcgcc ctggttgagg aacgggaagg cgctgtcgcc
     8461 ggacacctgc tggtagccgg cactcagcgc gtggccgccc agggtgtagg tgaacagggc
     8521 gctccaggtc tggttgtcga cctcgccgtt gtcggggttg ttggtgccac cgttccagta
     8581 gccgctgcta cggtagccct cggcacgccc ggcggcgctg gaattcttgc cgtcggaact
     8641 gctgtcgaaa tagcgcaggt cgctcttcag cgcgcccacc ggcagcgccc agttgtgcac
     8701 caggccgagg aagtgctggt cgtagaagtc ttccaggttg ccgtagtagt actgcgcggt
     8761 caggtccttg gtcagcttgt agtcgccgcc ggcgaagtag aacttgttgg tgtcggcctg
     8821 aataccgttg accgctgtgg cgccggcgat gcgcaggcca atgtcgtcgg tggagttgcg
     8881 ggtcttggtg tgctccagct ggccggtggt cagggtcaga ccatcgatct cgttgctgat
     8941 gatctgcccg ccttcgaaca gctgcggcag caggcggccg tcgttgaagg tcaccaccgg
     9001 cagcttgggc tgcagggtgc cgaggcgcgc ttcggtcttc gacagcttga ccttggcggt
     9061 cagcccggcc ttgctgtact cgtgcacggc cttcccgttg ctttcagtgg ggaacagctg
     9121 gcccggcttg cgctggtcgg ttctgtcgcc gtcaccgccg ccgtccaggc gcacgccgag
     9181 caggccgacg gcatccacgc cgaagccgac ggtgccttcg gtgaagccgg actggtagtt
     9241 gaagatgaag ccctggcccc actcgctggc ttcgttgcgg ttgtcggcgg tgttcttgtc
     9301 gttgcggtcg tagtagaagt tgcgcagggc caggctggcc ttgctgtcct cgaggaaggc
     9361 ggcctgggcc gcgccggaca gggcggaggc cagggtggcg gcgaacacgg cgagggccag
     9421 gggggacttg ttcattgcgt gtttctccat gaaatagacg gggcgtgtga ctgggacgcc
     9481 ccgggcgtta gcgagcgagc tataccgccg cgccgaaaaa tgacggtttg ttgcagcctt
     9541 atgtcggttg cgaaggtact ggagacctga taaatccaaa aagaataatt atttattttt
     9601 aaatgctatt agtgaataag aggtgcgagc cagcaggccc gcttcgatca gcagccccgg
     9661 ctgccggaag atcgggcgtc gagcagtgcc tgcgccgcaa gcgcggttgt tcatggtgtt
     9721 ctccttcgat cggtggcaat cggtaaacag ggcgccttga tcagggcagg agaagctagg
     9781 caaaagggtt gccagccgcg aaatctttga atttaaacct tttaaatcaa cgagttggta
     9841 tttgtcggta gggcgggaca acgcccgctg ggcctgtcgg ttgggcgctg aagtcggatt
     9901 ggttatgtag ttttgtatac aacgctacag gcgggcgggc aggcagggta acccggcagc
     9961 gcggcagggc cggctgccgg aggcggctta gatcaccgac ggcccgcgag ggtattgcat
    10021 gagcgttccc gacgtttccc cggcgcctgc tcgctggccg ccgcagggct aggctgaact
    10081 cagcgcccgc agctcaccgg gctcctgccg cccgcgtgct gcgggagctg cggcttatcg
    10141 agtaccctgc ctttctgtcc ttctctccac aggagccccc cgatgatcta ccgccctctc
    10201 ggcaaaaccg gccttcaggt cagcgcgctg gcgctcggca ccatgacctg gggcgagcag
    10261 aacaccgagg ccgaagcctt cgagcagatc cgccgcgccc aggctgccgg gatcaccttc
    10321 atcgataccg ccgagatcta cccggtgccg ccgaaggccg agaccgccgg tgccaccgaa
    10381 accatcatcg gcaactactt ccagcgccac ggcgggcgca gcgactggat cctcgccagc
    10441 aaggcggcgg cgccgggcaa cggcatcacc cacatccgcg gcgggcgcaa ccacttcgac
    10501 cgcgcca
//
LOCUS       DATCUN010000153         5857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_62456, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000153 DATCUN010000000
VERSION     DATCUN010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62456"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VIO83_06180"
     CDS             <1..784
                     /locus_tag="VIO83_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8330896.1"
                     /translation="PAQAEGFLARLLDQPVPGGVAVVDLGTGAQRPAARYQGKPVLVT
                     REDGRRWIAVVGLPLTVKPGREQLELADGRRLAFEVGSRHYNEQRITLKNQQQVTPNA
                     ANLKRIERELAVQTAAYQQFSPRQPSNLLFDKPVGGPLSSPFGLRRFFNGEERNPHSG
                     LDFAVPAGTPVKAPAAGKVILVGDYFFNGRTVFVDHGQGLISMFCHLSRIGVKVGDEL
                     ARGAVLGKVGATGRATGPHLHWNVSLNDARVDPSIFIGAFQR"
     gene            815..2059
                     /locus_tag="VIO83_06185"
     CDS             815..2059
                     /locus_tag="VIO83_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY8330897.1"
                     /translation="MANQLASWLDWLQRQPLLLTLIASSLLLAVAWLANWLVKHILVR
                     GLYKALSTTELGRQTRFQDHGVIRRLANVVPALILSIGVELVPGLPKALVTVVHNVSN
                     ALIVLAIVLALSALLDVANTLYLRRRDAHLRPIKGYLQVLKIAAFAIATVLMIAILVD
                     RSPLILLSGLGAMAAVLMLIFQDTLLSLVASVQISSNDLVRVGDWVEMPQLNADGDVV
                     DIALHTVKVQNWDKTITSIPTKRFITDSFKNWRGMQESGGRRIKRSLFLDQNSVHFLD
                     AGEIEHLRTFALLKDYLRGKLGELEEWNRRLAEQGEAPLNQRRVTNIGTFRAYVENYL
                     RHHARLRQDMTLLVRQLSPTADGLPLELYCFTNTTAWVQYEAIQADIFDHLLAILPEF
                     GLRVFQHPSGADLRTWRAPPQG"
     gene            complement(2094..4268)
                     /locus_tag="VIO83_06190"
     CDS             complement(2094..4268)
                     /locus_tag="VIO83_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent copper receptor"
                     /protein_id="HEY8330898.1"
                     /translation="MSVFARPSLAQSRLSRPFALNPFQRRWWPAHAALLGVLALPLQA
                     AELEEGAAAAEDEIAPLVITSVAPKSPLTVVTNPKQPRQPVPASDGADYLKTIPGFAA
                     IRNGGVNGDPVFRGMFGSRIKLLTNGGEMLGACPGRMDSPSSYISPESYDRLTVIKGP
                     QSVLWGPGASAATVLFEREPEQFSEPDWRLNSSLLAGSNGRFDRSIDAAAGSQDGYAR
                     IMANRSQADDYENGDGDTVQSRFNKWNTDVALGWTPDANTLLELTAGTGDGEARYAGR
                     SMDGSQFERESLGLRFKKTYIGETFHGLEAQVYYNYADHVMDNFSLRDPDPAASMAMM
                     RNPMASNVDRRTLGGRVAGTWRWDGVELVGGVDAQRNEHRKRASSYNMMTGTYTDADE
                     FAWNKDADFHNYGLFGELTWKLDQRQRLIGGARLDRAEAKDYRQTHTSHNPMTMKSQT
                     WANPSADESRHDTLYSGFLRYERDLQSLPATAYVGLGHSERFPDYWELFSASAGPVGS
                     IQAFETVKPEKTTQLDFGLQYRQGPLEAWASAYVGVVEDYILFDYVPGIMPGMTRTMV
                     DNIDARIAGAELGASYRFTPNWKGDASLAYAWGENDSDNRAMPQIPPLEARLGLTYER
                     DNWSTTGLWRVVAAQNRIAEGQGNVVGKDFDESAGFGVLSFNGAYRLSKNLKLSAGVD
                     NLLDKNYSEHLNLAGNAGFGFPADTRIDEPGRTYWTRLDLSF"
     gene            complement(4357..4719)
                     /locus_tag="VIO83_06195"
     CDS             complement(4357..4719)
                     /locus_tag="VIO83_06195"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 domain-containing protein"
                     /protein_id="HEY8330899.1"
                     /translation="MRLAAWLGLFAMLMAFAGPLYSQLRALDSLRLSLAIDLEALHCA
                     AEQPFLDDQGPAEWVQSLEQCGYCNLLAHTPSLPPSLQLALPAPSSHVHQYLPPSESL
                     PQAPPASRHARAPPALHA"
     gene            4741..5022
                     /locus_tag="VIO83_06200"
     CDS             4741..5022
                     /locus_tag="VIO83_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330900.1"
                     /translation="MPPPWKELRSQSPLQALRMIAQRHASHLVRHGAAQASLPAPARV
                     SRAKPPRKKTEEFAPKTKSNSAEKTSRVKYANFSTMLAIHAQRTLGFAP"
     gene            5157..>5857
                     /locus_tag="VIO83_06205"
     CDS             5157..>5857
                     /locus_tag="VIO83_06205"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY8330901.1"
                     /translation="MPMLKDPSQKYRAFPQIDIPDRTWPSKTITQAPIWLSSDLRDGN
                     QSLIEPMDADKKLRMFQILVQVGVKEIEVGFPSASQTDFDFVRTLIEGGHIPDDTTIQ
                     VLTQAREDLIVRTFESLKGAKKAIVHYYNATAPSFRKIVFHQDQAGVTQIAINAAQII
                     KRQAAQYPETDWRFEYSPEVFSSTETPFAVEVCNAVIGVFQPTPQQKLILNLPATIEA
                     ATPNVYADQIEWFGR"
BASE COUNT          944 a         2049 c         1927 g          937 t
ORIGIN      
        1 gccggcgcag gccgagggct tcctcgcccg tctgctggac cagccggtgc ccggcggcgt
       61 ggcggtggtc gatctcggca ccggcgcgca acgccccgcg gcccgttacc agggcaagcc
      121 ggtgctggtg acccgggaag acggccggcg ctggatcgcc gtggtcggcc tgccgctgac
      181 ggtcaagccg ggccgcgagc agctcgaact ggccgacggc cgccgcctgg ccttcgaggt
      241 cggcagccgc cactacaatg aacagcgcat caccctgaag aaccagcagc aggtcacgcc
      301 caacgccgcc aacctcaagc gcatcgagcg cgagctggcc gtgcagaccg cggcctacca
      361 gcagttcagc ccgcgccagc cgagcaatct gctgttcgac aagccggtcg gcggcccgct
      421 gtccagcccg ttcggcctgc gccgcttctt caacggcgag gaacgcaacc cgcactcggg
      481 cctcgacttc gccgtgccgg ccggcacccc ggtcaaggcg ccggcggcag gcaaggtgat
      541 cctggtcggc gactacttct tcaacggccg caccgtgttc gtcgaccacg gccaggggct
      601 gatcagcatg ttctgtcatc tgtcacggat cggcgtcaaa gtcggcgacg agcttgcgcg
      661 cggtgcggtg ctcggcaagg tcggcgccac tggccgcgcc accgggccgc acctgcactg
      721 gaacgtcagc ctcaacgacg cgcgggtcga cccgagcatc ttcatcggcg cgttccagcg
      781 ctgaccacgc gccggggcag accaggagtt atccatggcc aaccaactcg ccagctggct
      841 cgactggctg cagcgccagc cgctgctgct caccctgatc gcctccagcc tgttgctggc
      901 ggtcgcctgg ctggccaact ggctggtcaa gcacatcctg gtgcgcggcc tgtacaaggc
      961 gctcagcacc accgaactgg gccggcagac gcgcttccag gaccatgggg tgatccgccg
     1021 gctggccaac gtggtgccgg cgctgatcct ctccatcggc gtcgagctgg tccccggcct
     1081 gcccaaggcg ctggtgaccg tggtgcacaa cgtcagcaac gcgctgatcg tgctggccat
     1141 cgtcctggct ctcagcgccc tgctcgacgt ggccaacacc ctgtacctgc ggcgccgcga
     1201 tgcccacctc aggccgatca agggctacct gcaggtcctc aagatcgccg ccttcgccat
     1261 cgccacggtg ctgatgatcg ccatcctggt cgaccgctcg ccgctgatcc tgctctccgg
     1321 cctgggcgct atggcggcgg tgctgatgct gatcttccag gacaccctgc tctccctggt
     1381 ggccagcgtg cagatttcct ccaacgactt ggtccgcgtc ggcgactggg tggagatgcc
     1441 gcagctcaac gccgacggcg acgtggtcga catcgccctg cacacggtca aggtgcagaa
     1501 ctgggacaag accatcacta gcatcccgac caagcgcttc atcaccgact cgttcaagaa
     1561 ctggcgcggc atgcaggagt ccggcgggcg acggatcaag cgcagcctgt tcctcgacca
     1621 gaacagcgtg cacttcctcg atgccgggga gatcgaacac ctgcgcacct tcgccctgct
     1681 caaggactat ctgcgcggca agcttggcga actggaggag tggaaccgcc gcctcgccga
     1741 gcagggcgag gcgccgctca accagcgccg ggtcaccaat atcggcacct tccgtgccta
     1801 cgtggagaac tacctgcgcc accacgcgcg cctgcgccag gacatgacgc tgctggtgcg
     1861 ccagctcagc cccaccgccg acggtctgcc gctggagctg tactgcttca ccaacaccac
     1921 cgcctgggtg cagtacgaag ccatccaggc ggacatcttc gaccacctgc tggcgatcct
     1981 cccggagttc ggcctgcgcg tgttccagca cccgagcggc gccgacctgc gcacatggcg
     2041 cgccccgccg caggggtagc cccgccggct cctcgccagc ggggcgggcg ggatcagaag
     2101 ctcagatcca ggcgcgtcca gtaggtccgc cccggctcgt cgatccgcgt atcggcaggg
     2161 aagccgaaac cggcgttgcc ggccaggttg aggtgctcgg agtagttctt gtccagcagg
     2221 ttgtcgaccc cggcgctgag cttgaggttc ttgctcagcc ggtaggcgcc gttgaacgac
     2281 agcacgccga agcccgcgct ttcgtcgaag tccttgccga ccacgttgcc ctgcccctcg
     2341 gcgatgcggt tctgcgccgc caccacgcgc cacaggccgg tggtgctcca gttgtcacgc
     2401 tcgtaggtca gccccaggcg cgcttccagc ggcgggatct gcggcatggc gcggttgtcc
     2461 gagtcgttct cgccccaggc gtaggccagg ctggcgtcgc ccttccagtt gggggtgaag
     2521 cggtagctgg cgcccagctc ggcgccggcg atgcgcgcgt cgatgttgtc gaccatggtg
     2581 cgggtcatgc cgggcatgat ccccggtacg tagtcgaaca ggatgtagtc ttccaccacc
     2641 ccgacatagg ccgaggccca ggcttccagc ggcccctggc gatactgcag gccgaagtcg
     2701 agctgggtgg tcttctccgg cttgaccgtc tcgaacgcct ggatgctgcc caccgggccg
     2761 gcgctggcgg agaacagctc ccagtagtcg gggaagcgct cgctatggcc gagaccgacg
     2821 taggcggtgg ccggcaggct ctgcaggtcg cgctcgtagc gcaggaagcc gctgtacagg
     2881 gtgtcgtggc ggctctcgtc ggcgctgggg ttggcccagg tctgcgactt catggtcatc
     2941 gggttgtggc tggtgtgggt ctgccggtag tccttggcct cggcgcggtc caggcgcgcc
     3001 ccgccgatca ggcgctgccg ctggtcgagc ttccaggtca gctcgccgaa caggccgtag
     3061 ttgtggaagt cggcgtcctt gttccaggcg aactcatcgg catcggtata ggtgccggtc
     3121 atcatgttgt agctggaggc gcgcttacgg tgctcgttgc gctgcgcatc gacgccgccg
     3181 accagctcga cgccgtccca gcgccaggtg ccggccaccc ggccgccgag ggtgcggcgg
     3241 tcgacgttgc tggccatcgg gttgcgcatc atcgccatac tggcggccgg gtccggatcg
     3301 cgcaggctga agttgtccat cacgtggtcg gcgtagttgt agtagacctg cgcctcgagg
     3361 ccgtggaagg tctcgccgat gtaggtcttc ttgaagcgca ggccgaggct ttcgcgctcg
     3421 aactggctgc cgtccatgct gcgcccggcg tagcgcgcct cgccgtcgcc ggtgccggcg
     3481 gtcagctcga gcagggtgtt ggcgtccggc gtccaaccca gggccacatc ggtgttccac
     3541 ttgttgaagc gcgactgcac ggtatcgccg tcgccgttct cgtagtcgtc ggcctgcgag
     3601 cggttggcca tgatccgcgc gtaaccgtcc tggctgccgg cggcggcgtc gatgctgcgg
     3661 tcgaagcggc cgttggagcc ggccagcagg ctgctgttca ggcgccagtc cggctcgctg
     3721 aactgctccg gctcgcgctc gaacagcacg gtcgccgccg aggcgcccgg cccccacagc
     3781 acgctctgcg ggcccttgat cacggtcaga cggtcgtagc tttccgggct gatgtaggag
     3841 ctcggcgagt ccatgcgtcc cgggcaggcg ccgagcatct cgccgccgtt ggtcagcagc
     3901 ttgatccgcg agccgaacat gccgcggaac accgggtcgc cgttgacccc gccgttgcgg
     3961 atggcggcga agccggggat ggtcttcagg tagtcggcgc cgtcgctggc cggcaccggc
     4021 tggcgcggct gcttgggatt ggtcaccacg gtcagcggcg acttcggtgc cacgctggtg
     4081 atcaccagcg gcgcgatctc gtcttcggcg gcagcggccc cctcctccag ctcagccgcc
     4141 tgcaacggca gcgccagcac ccctagcagc gcggcatggg ccggccacca gcggcgctgg
     4201 aacggattga gggcgaacgg acgggacagg cgcgactgcg ccagggacgg acgagcgaaa
     4261 acggacatgt tggtattcca cgcaaaagac ggactgcacg tcgtcaggcg gatgcgccgc
     4321 agcgcaccgg caggcagacg accggttcag gacgggtcag gcgtggagag cggggggcgc
     4381 gcgcgcgtgc cggctggccg gcggtgcctg gggcaggctc tcgctgggcg gcaggtattg
     4441 gtgaacgtgg ctggagggcg ccggcagggc cagctgcagg ctcggcggca gcgacggggt
     4501 atgcgccagc aggttgcagt agccgcactg ctcgaggctc tgcacccact cggccgggcc
     4561 ctggtcatcg aggaacggct gttcggcagc gcagtgcaac gcctcgaggt cgatggccag
     4621 cgacagccgc aggctgtcca gcgcccgcag ctgcgagtac agcggcccgg cgaacgccat
     4681 gagcatggcg aacaggccaa gccaggctgc cagacgcatg cgcccttgcc gaatcacccg
     4741 atgccgcctc cctggaaaga attgcgcagc cagtcaccgc tgcaagcgct gcggatgatc
     4801 gcacagcgcc acgccagcca cctagtgcga cacggggccg cacaggcctc cctgccggcg
     4861 ccggctcgcg tcagccgcgc caaaccaccc cgcaagaaaa ccgaagaatt cgcgccaaaa
     4921 acgaaatcca acagcgcaga aaaaacatca cgagttaaat atgccaattt ttcaacaatg
     4981 cttgccattc atgcgcagcg gacgctaggg tttgcgccat gaacactgcc cacaccctca
     5041 tcctgcagcg gcaataccag tgccagcacc tggtcagtcc gcgactgcgg ggctgaagcg
     5101 gcgccgttca cccctcttgc ccgccgccct gcggctaccg ttttcaggaa ttcgccatgc
     5161 ccatgctcaa agatccgtcg cagaagtacc gcgccttccc gcagatcgac attcccgatc
     5221 gcacctggcc gtcgaagacc atcacccagg cgccgatctg gctgagctct gacctgcgcg
     5281 acggcaacca gtcgctgatc gagccgatgg atgccgacaa gaagctgcgc atgttccaga
     5341 tcctcgtgca ggtcggcgtc aaggaaatcg aggtgggctt cccgtccgcc tcgcagaccg
     5401 acttcgactt cgtgcgcacc ctgatcgagg gcggccacat tcccgacgac accaccatcc
     5461 aggtgctcac ccaggcccgc gaagacctga tcgtgcgcac cttcgagtcg ctcaagggcg
     5521 ccaagaaagc catcgtccac tactacaacg ccaccgcgcc gagctttcgc aagatcgtct
     5581 ttcatcagga ccaggccggg gtgacccaga tcgccatcaa cgcggcgcag atcatcaagc
     5641 gccaggccgc gcagtacccg gaaaccgact ggcgcttcga gtactcgccc gaggtgttca
     5701 gctccaccga gacgcccttc gccgtcgagg tgtgcaacgc ggtgatcggc gtgttccagc
     5761 cgaccccgca gcagaagctg atcctcaacc tgccggcgac catcgaggcg gccacgccga
     5821 acgtctacgc cgaccagatc gagtggttcg gccgcca
//
LOCUS       DATCUN010000154        11487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_119151, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000154 DATCUN010000000
VERSION     DATCUN010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11487
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119151"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..1247
                     /locus_tag="VIO83_06210"
     CDS             336..1247
                     /locus_tag="VIO83_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8330902.1"
                     /translation="MNLRHSWAAAGLVLSSLCWAGNALVARAVADSIPPMALAFWRWS
                     LALAILLPFIAGPLWQQRAVVRGAGWRLWLVAGLGIASYNSLLYHAAHSTSAINITLL
                     NTCLPLATFLGAGVLLGQWPARRAWFGMALAACGLLLLIGRGDLATFRSLDFYRGDLL
                     MLLAVVAWALYSLLIRLWAPRLALPPLALLGVMILFGLLLMLPLYLLELTRVGGFMVT
                     PRNVAAIGYTALFASLLAYLSWNHGLKVLGAARASLFSYLMPVFAALLGWLLLGEQLA
                     FYHWLGGALIFAGLLLATRPVVANGTT"
     gene            complement(1244..1714)
                     /locus_tag="VIO83_06215"
     CDS             complement(1244..1714)
                     /locus_tag="VIO83_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330903.1"
                     /translation="MHRTRTLLPPRSGSVARLLLLTCLAGWLPGASAQQPAPATPRQT
                     AFDDNNYRPGEIVNRLPPPPPTARVRRSPPPATTTRSAKWSWKTRQSLAHGTFQWREQ
                     NGRIDYASVCMNEQPGSLRYRDCRKGAKVAFARLCKARRDPAACHAQNNYSALR"
     gene            complement(1830..1906)
                     /locus_tag="VIO83_06220"
     tRNA            complement(1830..1906)
                     /locus_tag="VIO83_06220"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1870..1872),aa:Met,seq:cat)
     gene            complement(2064..3164)
                     /gene="ychF"
                     /locus_tag="VIO83_06225"
     CDS             complement(2064..3164)
                     /gene="ychF"
                     /locus_tag="VIO83_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY8330904.1"
                     /translation="MGFNCGIVGLPNVGKSTLFNALTKSGIAAENFPFCTIEPNSGIV
                     PMPDARLDALAEIVKPERVLPTTMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIAH
                     VVRCFEDDNVIHVSNSVDPKRDIEIIDLELIFADLDSCEKQLQKVTRNAKGGDKEALA
                     QKAILEQLIPHFTEGKPARTLMKNMSSDEKALIRGFHLLTSKPVMYIANVAEDGFENN
                     PLLDVVKAIAEEEGAIVVPVCNKIEAEIAELEDGEEKDMFLESLGLEEPGLNRVIRAG
                     YQLLNLQTYFTAGVKEVRAWTVKVGATAPQAAAVIHTDFEKGFIRAEVIAYDDFIQYK
                     GEAGAKEAGKWRLEGKEYIVKDGDVMHFRFNV"
     gene            complement(3206..3790)
                     /gene="pth"
                     /locus_tag="VIO83_06230"
     CDS             complement(3206..3790)
                     /gene="pth"
                     /locus_tag="VIO83_06230"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY8330905.1"
                     /translation="MTAIQLIVGLGNPGPEYEQTRHNAGALFVERVASAQGVNLATDK
                     KYFGLVGKFSHQGRDVRLLIPTTYMNRSGQSVAALANFFRIPPAAILVAHDELDMPPG
                     TAKLKVGGGHGGHNGLRDIIAQLGNQNSFYRLRLGIGHPGDKNLVSGFVLGRAPRSEQ
                     EKLEASIDFALDVLPEMLAGDWTKAMQKLHGQKA"
     gene            complement(3825..4412)
                     /locus_tag="VIO83_06235"
     CDS             complement(3825..4412)
                     /locus_tag="VIO83_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEY8330906.1"
                     /translation="MNNFALNAEARSDLGKGASRRLRRNASLVPAVIYGGEAAPLSIS
                     LLAKDFAKLLENEAVFSHVLALNVDGNVETVVIKALQRHPAKGFVLHADFVRVVADHK
                     LNATVPLHFINAESADAVKLQGGEISHTMSEVEVSCLPKDLPEFIEVDLAKVAIGQTV
                     HLSDLTVPAGVELVALAHGNDLAIANIHASRVQGE"
     gene            complement(4519..5460)
                     /locus_tag="VIO83_06240"
     CDS             complement(4519..5460)
                     /locus_tag="VIO83_06240"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY8330907.1"
                     /translation="MSKMMVFTGNANPDLARRVVRQLHIPLGDVSVGKFSDGEISVEI
                     NENVRGKDVFLIQPTCAPTNDNLMELVVMADAFRRSSASRITAVIPYFGYARQDRRPR
                     SARVAISAKVVADMLTVVGVDRVLTVDLHADQIQGFFDVPVDNIYGSPTLVDDIQAQR
                     FENLMIVSPDIGGVVRARAVAKSLGVDLAIIDKRRPKANVSEVMHIIGDVEGRTCVLV
                     DDMVDTAGTLCHAATALKKHGAAKVYAYCTHPVLSGRAIENIENSVLDELVVSNTIPL
                     SAAAQACDRIRQLDIGPVVAEAVRRISNEESISAMFR"
     gene            complement(5557..5631)
                     /locus_tag="VIO83_06245"
     tRNA            complement(5557..5631)
                     /locus_tag="VIO83_06245"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5597..5599),aa:Gln,seq:ttg)
     gene            complement(5680..5754)
                     /locus_tag="VIO83_06250"
     tRNA            complement(5680..5754)
                     /locus_tag="VIO83_06250"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5720..5722),aa:Gln,seq:ttg)
     gene            complement(5773..6639)
                     /gene="ispE"
                     /locus_tag="VIO83_06255"
     CDS             complement(5773..6639)
                     /gene="ispE"
                     /locus_tag="VIO83_06255"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY8330908.1"
                     /translation="MHEATLILPAPAKLNLFLHILGRRPDGYHELQTVFQFLDYGDEL
                     GFRLRDDGEIHLHTAIAGVPHDSNLIVRAARRLQQEAGTPLGADIWLDKRLPMGGGIG
                     GGSSDAATTLLGLNHLWQLDLDLDRLATLGLELGADVPVFVRGHAAFAGGVGEQLAPV
                     DLEEPWFLVAVPQVSVSTAEIFCAPELTRDTPAIRVCGLPEGGGHNDCQPVVEQRYPE
                     VRNALNLLNKFVPARMTGTGACVFGSFPNHCDADKVSRQLPATLPSFVAQGRNVSMLH
                     RTLQALVQEVNA"
     gene            complement(6662..7279)
                     /gene="lolB"
                     /locus_tag="VIO83_06260"
     CDS             complement(6662..7279)
                     /gene="lolB"
                     /locus_tag="VIO83_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HEY8330909.1"
                     /translation="MNLRGLLLLAGLLLLAGCAGLGPREDVGGQGTASAWRQHKTEVA
                     TIDGWQIDGKVGIRAPQDSGSGVLFWLQRRDYYDIRLSGPLGRGATRLTGREGDIRLE
                     VANQGRYQAESPEALLEEQIGWRLPVSHLLWWIRGLPAPDSKSRLTLNADSRLARLEQ
                     DGWRVEYADYQQQNGYWLPQRLKLFGENLDITLVIKAWQPRRLGL"
     gene            complement(7276..9003)
                     /locus_tag="VIO83_06265"
     CDS             complement(7276..9003)
                     /locus_tag="VIO83_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8330910.1"
                     /translation="MNKSLALLTALALLGGCQALPSREPDATPPAAATDTPPVAAPAP
                     SASFSPDTLYSLLVAEIAGQRDRFDIALDNYVEQARSTDDPAVTERAYRIAEYLGADD
                     AALETAILWANSAEGNLDAQRAAAIQLARAGRHEEAMLYMEQVLQAKGDTHFDFLALT
                     AAQTDPETRAGLLQSFERLLERHPDNAQLLFGRAVLLQQDGRSEEALTSLEQHPAGSR
                     EVAPLLMRARLLRSLERGEEALPLLKEGLRLHPDDKRLRLAYARELLQEQRLDEARAQ
                     FRELLQRFPEDDDLRLSLALVSLEAKAWDDARRLLRELIERDAHVDTAHFNLGRLAEQ
                     DSDSAAALREYALVGPGNDFLPAQVRRTELLVKAGRSAEASRLLASERDSQPDYALQL
                     YLIEIESLTNQERLGDAWQLITQALEQYPEDLNLLYTRAMLAEKRGDLSGLERDLRTI
                     IEREPDNAMALNALGYTLADRTTRYAEAHALIEKAYALNPTDPAILDSLGWVNYRLGN
                     LAEAEKLLRQALARMPDHEVAAHLGEVLWAAGKQDEARQVWRQALAETPDSAVLRDTL
                     KRLTGKVKP"
     gene            9182..10450
                     /gene="hemA"
                     /locus_tag="VIO83_06270"
     CDS             9182..10450
                     /gene="hemA"
                     /locus_tag="VIO83_06270"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY8330911.1"
                     /translation="MAFLALGINHKTASVAVRERVAFGPEQLVDALQQLCRVTPCREA
                     AILSTCNRSELYLALDEAQAEAILAWLAGYHGLSLDELRACAYIHQDNEAVRHMMRVA
                     AGLDSMILGEPQILGQMKDAYASAREAGTLGPLLGRLFQATFSTAKTVRTDTRIGENP
                     VSVAFAAVSLAKQIFSDLPRSQALLIGAGETITLVARHLHEQGIKRIVVANRTLERAS
                     LLAEEVGGQAVLLADIPEQLVNSDIVISSTASPLPILGKGAVERALKKRKHRPVFMVD
                     IAVPRDIEPEVGELDDVYLYTVDDLHEVIAENLRSRQGAARAAEELVLTGVDEFMARL
                     RERDSVDLLRSYRQRAEQLRDDELAKALRQLGNGSPAEEVLAQLARGLTNKLLHAPSV
                     QLKKFSAAGRHDALAVAQELLDLDGAGQHR"
     gene            10508..>11487
                     /gene="prfA"
                     /locus_tag="VIO83_06275"
     CDS             10508..>11487
                     /gene="prfA"
                     /locus_tag="VIO83_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY8330912.1"
                     /translation="MKASLLNKLDTLQDRFEELTALLGDAEVIGDQTKFRAYSREYAE
                     IEPVNAAFRAFRKVQGDLEGAQALLKDSDPDLREMAEEEVTEAKAQLEVLESQLQRML
                     LPKDPNDGRNVFLEIRAGTGGDEAAIFSGDLFRMYSRYAERQGWRVEILSESEGEHGG
                     YKEVIARVEGDNVYAKLKFESGAHRVQRVPETESQGRIHTSACTVAVLPEPDEQAAIE
                     INPADLRVDTYRASGAGGQHINKTDSAVRITHLPTGMVVECQEERSQHKNRARAMGWL
                     AARLQDQQDAAAHKEISETRKLLVGSGDRSERIRTYNFPQGRVTDHRINLT"
BASE COUNT         1784 a         3671 c         4036 g         1996 t
ORIGIN      
        1 ctgtggtcgg ccgggggctg gagcctgctg gcgccacggc tgtgccggcc gctggggcgc
       61 gtgcatttcg ccggcgccga tctggcacgg cgctggccgg gcacactcga gggcgcggtg
      121 gaggctggcg aacaggcggc cgaggaagtg ctcggcctcg gttgaacggc gggcgagttt
      181 gacttgccaa gacggtgagg caattcgtaa ggtgccgggc tggtctgcca ggagtcgtcc
      241 atgcctttcc cccaagtccc accgcgccgc cccgagcgct gcggcccttg ctccgctgcc
      301 cggtactcgt ggtcgttgcc cgccagggcg tgccgatgaa cctgcgtcac agctgggccg
      361 ccgccgggct ggtgctgtcc agcctgtgct gggccggcaa tgcgctggtg gcccgggcgg
      421 tggctgacag catcccgccg atggcgctgg cgttctggcg ctggtcgctg gcgctggcca
      481 tcctcctgcc cttcattgcc ggccccctgt ggcagcagcg cgctgtcgtg cgtggcgccg
      541 gctggcgcct gtggctggtc gccgggctgg gcatcgccag ctacaactcg ctgctctacc
      601 acgcggcgca cagcacctcg gcgatcaaca tcaccctgct caacacctgc ctgccactgg
      661 cgaccttcct cggtgccggc gtgctgctcg gccaatggcc ggcgcggcgt gcctggttcg
      721 gcatggcgct ggcggcctgc ggtttgttgc tgttgatcgg ccgtggcgat ctggcgacct
      781 ttcgcagcct cgacttctat cgcggcgact tgctgatgct gctggccgtg gtggcctggg
      841 cactgtactc gctgctgatc cgcctgtggg cgccgcgcct ggcgctgccg ccactggccc
      901 tgctgggcgt gatgatcctg ttcggcctgc tgctgatgct gccgctctac ctgctcgagc
      961 tgacccgcgt cggcggcttc atggtcacgc cgcgcaacgt ggctgccatc ggctatacgg
     1021 cgctgttcgc ctcgctgctc gcctacctga gctggaacca cgggctgaag gtgctcggcg
     1081 cggcgcgcgc ctcgctgttc agctacctga tgccggtgtt cgccgcgctg ctcggctggc
     1141 tgctgctggg cgaacagctg gccttctacc actggctggg cggtgcgctg atcttcgccg
     1201 gcctgttgct ggccacccgc ccggtcgtgg cgaacggcac gacctagcgc agcgccgagt
     1261 agttgttctg cgcatggcag gcagccgggt cgcggcgcgc cttgcacagc ctggcgaagg
     1321 cgaccttggc ccccttgcgg cagtcgcgat agcgcaggga gccgggctgc tcgttcatgc
     1381 acacgctggc gtagtcgatg cgcccgttct gctcgcgcca ctggaaggtg ccgtgcgcca
     1441 ggctctggcg ggttttccag gaccacttgg cggagcgggt ggtggtggcg ggcggcgggc
     1501 tgcgcctgac ccgggccgtc ggaggtggcg gcggcaggcg gttgacgatc tcgccagggc
     1561 gatagttgtt gtcgtcgaac gccgtctgtc gcggcgtcgc tggggcaggc tgctgagcgc
     1621 tggcgccggg gagccagccg gcgaggcatg tcagcagcag aagtcgcgca acgctgccgc
     1681 tgcggggcgg gagtagggtt cgggtgcggt gcatggtgcc tcctggcatg ggcaataccc
     1741 cggatccttc ggggcccctg cagcctagcc tgcacgtttt tccggcacaa cgaaaaaggg
     1801 cctgcattgc tgcaggccct tttggaattt ggtggctacg cagggacttg aaccccggac
     1861 cccagcatta tgaatgccgt gctctaacca actgagctac gtagccaacg gcgcgcatta
     1921 ttcgcgtcgg cggggctatt gtcaatccct gtcacgtaaa aaagttcaga cggatcaacg
     1981 gcttggctgt gcggcacgaa ttgcaccggt aaaaacagca agacccggca cacgggccgg
     2041 gtcttgcggg cgcgcggggc gactcagacg ttgaagcgga agtgcatcac gtcgccgtcc
     2101 ttgacgatgt actccttgcc ttccaggcgc cacttgccgg cttccttggc gccggcttcg
     2161 cccttgtact ggatgaagtc gtcataggcg atgacttcgg cgcggatgaa gcccttctcg
     2221 aagtcggtgt ggatcacggc ggcggcctgc ggcgcggtgg cgccgacctt gacggtccag
     2281 gcacgcactt ccttgacccc ggcggtgaag taggtctgca ggttgagcag ctggtagccg
     2341 gcgcggatca cccggttgag gcccggttct tccaggccca gcgactcgag gaacatgtcc
     2401 ttctcctcgc cgtcttccag ctcggcgatt tccgcctcga tcttgttgca taccggcacc
     2461 acgatggcgc cttcttcctc ggcgatggcc ttgaccacgt cgagcagcgg gttgttctcg
     2521 aagccgtcct cggcgacgtt ggcgatgtac atcaccggct tgctggtgag caggtggaaa
     2581 ccgcggatca gcgccttctc gtcgctgctc atgttcttca tcagggtgcg cgccggcttg
     2641 ccctcggtga agtgcgggat cagctgctcg aggatggctt tctgcgccag ggcttccttg
     2701 tcgccgccct tggcgttgcg ggtgaccttc tgcagctgct tctcgcagct gtcgaggtcg
     2761 gcgaagatca gttcgaggtc gatgatctcg atgtcgcgct tggggtcgac cgagttggac
     2821 acgtggatca cgttgtcgtc ctcgaagcag cgcaccacgt gggcgatggc gtcggtctcg
     2881 cggatgttgg cgaggaactt gttgcccagg ccttcaccct tggaggcgcc ggcgaccagg
     2941 ccggcgatgt cgacgaactc catggtggtc ggcagcacgc gctcgggctt gacgatctcg
     3001 gcgagggcgt cgaggcgcgc gtcgggcatc ggcacgatgc cgctgttcgg ctcaatggtg
     3061 cagaacggga agttctccgc ggcgatgccg gacttggtca gggcgttgaa cagggtggac
     3121 ttgccgacgt tgggcaggcc gacgatgccg cagttgaatc ccatggtgta tccctcgcgg
     3181 agatgagttc gatcgtggag atgagtcagg ctttctggcc gtgcagtttc tgcatggcct
     3241 tggtccagtc gccggcgagc atttccggca gcacgtcgag ggcgaagtcg atgctggcct
     3301 cgagcttttc ctgttcgctg cgcggagccc ggccgaggac gaagcccgag acgaggttct
     3361 tgtcgccggg gtggccgatg ccgagccgca ggcgatagaa ggaattctgg ttgccgagct
     3421 gggcgatgat gtccctgagc ccgttgtgcc cgccgtggcc gccgcccacc ttgagcttgg
     3481 cggtacccgg aggcatgtcg agttcgtcgt gggccaccag gatggccgct ggcggaatgc
     3541 ggaagaagtt cgccagcgcc gccacggact ggccgctgcg gttcatgtag gtggtgggga
     3601 tgagcagacg aacgtcgcgg ccctgatggc tgaacttgcc gaccaggccg aagtacttct
     3661 tgtcggtcgc gaggttgacg ccctgggcgg acgcgacgcg ctcgacgaaa agagcccctg
     3721 cgttgtgccg ggtctgttcg tattccggac cggggttacc gaggccgacg atcagttgaa
     3781 tggcagtcac gacaggggct ctcttggttc agtcaacgag tgacttattc gccttgcacg
     3841 cgggaagcgt ggatgttggc gatggccagg tcgttgccgt gcgccagggc aaccagctcg
     3901 acgccggccg gaacggtcag gtcggacagg tgtacggtct ggccgatggc caccttggcc
     3961 aggtcgactt cgatgaattc cggcagatcc ttcggcaggc aggaaacttc cacttccgac
     4021 atggtgtggg agatttcgcc accctgcagc ttcacggcat cagcgctttc ggcgttgatg
     4081 aagtgcagcg gtacggtagc gttcagcttg tggtcggcga caacgcggac gaagtcggcg
     4141 tgcaggacga agcctttggc cgggtggcgc tgcagggcct tgatcacgac ggtttcaacg
     4201 ttgccgtcga cgttcagggc gagcacgtgg ctgaagacag cctcgttttc cagcagcttg
     4261 gcgaaatcct tggccagcag gctgatggac agcggagcgg cttcgccacc gtagatcacg
     4321 gcaggaacca ggctggcgtt gcgacgcagg cggcggctcg cacctttccc caggtcggaa
     4381 cgcgcttcgg cattcagggc aaaattgttc attgcatttc tccagaatga caacacgccc
     4441 gagacgcttg cgaccagcgt ttcgggcggt tgactaaggc cccgcacggg gcggggcagt
     4501 tgtatcggcg ccggttcctt agcggaacat ggcgctgatg gattcttcgt tgctgatgcg
     4561 gcgtacggct tcggccacga ccggaccgat gtccagctgg cggatgcggt cacaggcctg
     4621 cgcagcggcg gacagcggga tggtgttgct caccaccagc tcgtcgagca ccgagttctc
     4681 gatgttctcg atggcgcggc cggacagcac cgggtgggtg cagtaggcgt agaccttggc
     4741 ggcaccgtgc ttcttcagcg cggtggcggc atggcacagg gtgccggcgg tgtcgaccat
     4801 gtcgtccacc agcacgcagg tacggccttc aacgtcgccg atgatgtgca tcacttcgga
     4861 aacgttggcc ttcgggcgac gcttgtcgat gatcgctaga tcgacgccca ggctcttggc
     4921 cacggcacgc gcacgcacca cgccaccgat gtccggggag acgatcatca ggttttcgaa
     4981 gcgctgggcc tggatgtcgt cgaccagggt cggcgagccg tagatgttgt ccaccggcac
     5041 gtcgaagaag ccctggatct ggtcggcatg cagatcgacg gtgagcacgc ggtcgacacc
     5101 gaccacggtc agcatgtcgg cgacgacctt ggcgctgatc gccacacggg cggagcgcgg
     5161 acgacgatcc tgacgggcat agccgaagta ggggatgacc gcagtgatgc gactggccga
     5221 ggagcggcgg aaggcatcgg ccatcaccac cagttccatc agattgtcgt tggtcggtgc
     5281 gcaagtcggc tgaatcagga aaacgtcctt gccgcggacg ttctcgttga tttcgacgct
     5341 gatttcgccg tcggagaact tgccgacgga aacgtcaccg aggggaatat gcaattgacg
     5401 cacgacacgg cgggcgagat cggggttggc gttccccgtg aagaccatca tcttggacac
     5461 gcgcagtacc tgccggctga gggtggacct ggatgaacaa aaaacctctg tgagctcgcg
     5521 gggttgccgc aaaaccacaa aggtttttcg agaatatggc aggggcggct ggattcgaac
     5581 caacgcatgg caggatcaaa acctgctgcc ttaccgcttg gcgacgcccc tgtggcgtaa
     5641 aagaatgctg atccggtgtt acgtaacctc gggaggttat ggcaggggcg gctggattcg
     5701 aaccaacgca tggcaggatc aaaacctgct gccttaccgc ttggcgacgc ccctgtatcg
     5761 tataaccgaa tgctaggcat tcacttcctg taccagtgct tgcagcgtgc ggtgcaacat
     5821 cgaaacattg cgcccctgag ctacgaagct tggcagagtg gccggaagct ggcgcgaaac
     5881 tttatcagca tcgcagtgat ttgggaagct cccaaacaca caagctccgg tgccggtcat
     5941 tcttgcagga acaaatttgt tcagcaagtt caaggcgtta cgtacttcag ggtaacgctg
     6001 ctcgaccacc ggctggcagt cgttatgacc gcccccttcg ggaaggccgc aaactctaat
     6061 ggccggggta tcccgtgtca actccggggc gcagaaaatt tctgctgtgc tgacagacac
     6121 ttgcggcacg gcaacgagaa accagggttc ctcgaggtcg accggggcga gctgctcgcc
     6181 gacgccgccg gcgaaggcag catggccgcg gacgaacacc ggcacgtcgg cgccgagttc
     6241 caggccaagg gtggccaggc ggtcgaggtc gaggtcgagc tgccacaggt ggttgaggcc
     6301 gagcagggtg gtggcggcat ccgagctgcc gccaccgata ccgccgccca ttggcaggcg
     6361 cttgtccagc cagatgtcgg cgcccagcgg cgtgccggct tcctgctgca ggcggcgcgc
     6421 ggcgcgcacg atcaggttgc tgtcgtgggg cacgccggca atcgccgtgt gcaggtggat
     6481 ttcgccgtcg tcgcgcaggc gaaagcccag ctcgtcgccg tagtcgagga actggaacac
     6541 cgtctgcagc tcgtggtagc cgtcgggacg gcggccgagg atgtgcagga acaggttgag
     6601 cttggccggg gccggcagga tcagggtagc ttcgtgcatg gcagtcatgg caagcgggta
     6661 ttcagaggcc gaggcggcgg ggctgccagg ccttgatcac cagggtaatg tcgagatttt
     6721 cgccgaacag cttgagccgc tgcggcagcc agtagccgtt ctgctgctgg tagtcggcgt
     6781 actcgacccg ccagccgtcc tgctcgaggc gcgccaggcg gctgtcggca ttcagggtca
     6841 ggcgactctt gctgtcgggg gcgggcaggc cgcggatcca ccacagcagg tgggagaccg
     6901 gcaggcgcca gccgatctgc tcctccagca gcgcctcggg cgattcggcc tggtagcggc
     6961 cctggttggc cacttcgagg cggatgtcgc cctcgcggcc ggtgagacgg gtggcgccgc
     7021 gaccgagcgg gccggacagg cggatatcgt agtagtcgcg ccgctgcagc cagaacagca
     7081 cgccgctgcc ggagtcctgc ggcgcacgga tgccgacctt gccgtcgatc tgccagccgt
     7141 cgatggtggc gacctcggtt ttgtgctggc gccaggcgct ggcggtgccc tggccgccga
     7201 catcctcgcg cgggccgagg ccggcacagc cggcgagcag cagcaggccg gcgagcagca
     7261 gcaggccgcg caggttcatg gcttcacctt gccggtcagg cgcttgagag tatcgcgcag
     7321 cacggcgctg tccggcgttt ccgccagcgc ctggcgccag acctggcggg cctcgtcctg
     7381 cttgccggcg gcccacagta cctcgccgag gtgggcggcg acttcgtggt cgggcatgcg
     7441 cgcaagggcc tggcgcagca gcttctcggc ctccgccagg ttgcccaggc ggtagttgac
     7501 ccagccgaga ctgtcgagga tcgccgggtc ggtcgggttc agcgcatagg ccttctcgat
     7561 cagcgcgtgg gcctcggcat agcgcgtggt gcggtcggcc agggtgtagc cgagggcgtt
     7621 gagcgccatg gcgttgtccg gttcgcgctc gatgatcgtg cgcaggtcgc gctccagccc
     7681 gctcaggtcg ccgcgcttct cggcgagcat ggcgcgggtg tagagcaggt tgaggtcttc
     7741 cgggtactgc tcgagggcct gggtgatgag ctgccaggcg tcgccgaggc gttcctggtt
     7801 ggtcaggctc tcgatctcga tcagatacag ctgcagcgcg tagtccggct ggctgtcgcg
     7861 ctcgctggcg agcaggcgac tggcttcggc actgcgcccg gccttgacca gcagttcggt
     7921 gcgacgcacc tgggccggca ggaagtcgtt gcccgggccg accagggcgt attcgcgcag
     7981 cgccgcggcg ctgtcgctgt cctgctcggc gaggcggccg aggttgaagt gggcggtgtc
     8041 gacatgggcg tcgcgctcga tcagttcgcg cagcaggcgg cgcgcatcgt cccaggcctt
     8101 ggcctccagg ctgaccagcg ccagcgacag gcgcaggtcg tcgtcctcgg ggaagcgctg
     8161 cagcagttcg cggaactgcg cccgcgcctc gtccagccgt tgctcttgca gcagctcgcg
     8221 ggcataggcc aggcgcaagc gcttgtcgtc ggggtgcagg cgcagtcctt ccttgagcag
     8281 cggcagggct tcctcgccgc gttcaagcga gcgcagcagg cgggcgcgca tcagcagcgg
     8341 ggcgacctcg cggctgccgg ccggatgctg ctcgaggctg gtcagggcct cctcgctgcg
     8401 gccgtcctgt tgcagcagca cggcgcggcc gaacagcagc tgggcgttgt ccggatggcg
     8461 ctcgagcagg cgctcgaagc tctgcagcag gccggcgcgg gtctccggat cggtctgcgc
     8521 ggcggtcagg gcgaggaagt cgaagtgggt gtcgcccttg gcctgcagca cctgctccat
     8581 gtacagcatg gcctcctcgt ggcgcccggc gcgggccagc tggatggcgg cggcacgctg
     8641 ggcgtcgaga ttgccctcgg cgctgttggc ccagaggatg gcggtttcca gcgcggcatc
     8701 atcggcaccg aggtattcgg caatgcgata ggcgcgttcg gtgaccgccg ggtcgtcggt
     8761 gctgcgcgcc tgctcgacgt agttgtcgag ggcgatgtcg aagcggtcgc gctgaccggc
     8821 gatttccgcc accagcaggc tgtacagggt gtccggactg aacgaggcgc tcggcgcagg
     8881 cgcggcgacc ggcggggtgt cggtcgccgc tgcgggcggg gtggcatcgg gttcgcggga
     8941 gggcagggcc tggcagccgc ccagcagggc gagggcagtc agcagcgcaa gggatttgtt
     9001 catggcaggc atcaggaggg ctcaccggca ggtcggccaa tcatgacaca tgcgcccggt
     9061 caatcccatg gtcgcagcgc gtcaagcagg gcgccgctaa cgacgatcgg cgcctggcag
     9121 ttgtcctgca ctggcctaag taggacaatt gcccgctttc aggccacctc agcatccccg
     9181 catggctttt ctcgcgctcg gcatcaacca caaaaccgca tcggtggcgg tccgcgagcg
     9241 cgtggctttt ggccccgagc aactggtcga cgccttgcag cagctgtgcc gggtgacgcc
     9301 gtgccgcgag gcggcgatcc tctccacctg caaccgcagc gagctgtacc tagcgctgga
     9361 cgaggcgcag gccgaggcga tcctggcctg gctggccggt taccacggcc tcagtctcga
     9421 cgagctgcgc gcctgcgcct acatccacca ggacaacgag gcggtgcgcc acatgatgcg
     9481 cgtggccgcc ggcctggact cgatgatcct cggcgagccg cagatcctcg gccagatgaa
     9541 ggatgcctac gccagtgcgc gcgaggccgg caccctcggt ccgctgctcg gccggctgtt
     9601 ccaggccacc ttcagcaccg ccaagaccgt gcgcaccgac acccgcatcg gagagaaccc
     9661 ggtgtcggtg gccttcgccg cggtcagcct ggccaagcag atcttcagcg acctgccgcg
     9721 cagccaggcg ctgctgatcg gcgccggcga gaccatcacc ctggtcgccc gccacctgca
     9781 cgagcagggc atcaagcgca tcgtggtggc caaccgcacc ctcgagcgcg ccagcctgct
     9841 ggccgaagag gtcggtggcc aggcggtgct gctggccgac attcccgagc agttggtcaa
     9901 cagcgacatc gtcatcagct ccaccgccag cccgctgccg atcctcggca agggcgcggt
     9961 cgagcgggcg ctgaagaagc gcaagcaccg tccggtgttc atggtcgaca tcgccgtgcc
    10021 gcgcgacatc gagccggaag tcggcgaact ggacgacgtc tacctgtaca ccgtcgacga
    10081 cctccacgag gtgatcgccg agaacctgcg cagccgccag ggcgccgccc gcgccgccga
    10141 ggaactggtg ctgaccgggg tcgacgagtt catggcgcgc ctgcgcgagc gcgacagcgt
    10201 cgacctgctg cgcagctatc gccagcgcgc cgaacagctg cgcgacgacg agctggccaa
    10261 ggcgctgcgc cagctcggca acggcagtcc ggccgaggag gtgctggccc agctggcgcg
    10321 tggcctgacc aacaagctgc tgcatgcgcc cagcgtgcag ctgaagaaat tttccgccgc
    10381 cggccgccac gatgcgctgg ccgtggccca ggaactgctc gacctcgacg gggccgggca
    10441 gcatcgctaa cgcccctgcg cgccgtggcg gtcaagccgc ggcgccagcc atggtgaaac
    10501 cacaccgatg aaagcctcgc tgctgaacaa gctcgacacc ctgcaggatc gtttcgagga
    10561 gctcaccgcg ctgctcggcg acgccgaggt gatcggcgac cagaccaagt tccgcgccta
    10621 ctcccgcgaa tatgccgaga tcgagccggt caacgccgcc ttccgcgcct tccgcaaggt
    10681 gcagggcgac ctcgagggcg cccaggcgct gctcaaggac agcgatcccg acctgcgcga
    10741 gatggccgag gaagaggtca ccgaggccaa ggcgcagctc gaggtgctgg agagtcagct
    10801 gcagcgcatg ctgctgccca aggaccccaa cgacgggcgc aacgtgttcc tcgagatccg
    10861 cgccggcacc ggcggcgacg aggcggcgat cttctccggc gacctgttcc gcatgtactc
    10921 gcgctacgcc gaacgccagg gctggcgggt cgagattctc tccgagagcg agggtgagca
    10981 cggcggctac aaggaggtga tcgcccgcgt cgagggcgac aacgtctacg ccaagctcaa
    11041 gttcgagtcc ggcgcccacc gcgtgcagcg cgtgccggaa accgagtcgc aggggcgcat
    11101 ccacacctcg gcctgcaccg tggcggtgct gccggagccg gacgagcagg cggccatcga
    11161 gatcaacccg gccgacctgc gcgtcgacac ctaccgcgct tccggcgccg gcggccagca
    11221 catcaacaag accgactcgg cggtgcgcat cacccacctg ccgaccggca tggtggtcga
    11281 gtgccaggaa gagcgctcgc agcacaagaa ccgcgcccgc gccatgggct ggctggcggc
    11341 ccggctgcag gaccagcagg acgccgccgc gcacaaggaa atctccgaga cgcgcaagct
    11401 gctggtcggt tcgggcgacc gttccgagcg catccgcacc tacaacttcc cgcagggccg
    11461 ggtcaccgac caccgcatca acctgac
//
LOCUS       DATCUN010000155         8394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_176163, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000155 DATCUN010000000
VERSION     DATCUN010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176163"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..1778
                     /locus_tag="VIO83_06280"
     CDS             372..1778
                     /locus_tag="VIO83_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY8330913.1"
                     /translation="MNTSTNAAHLPAGIFRAYDIRGVVGDSLTSETAYWIGRTIGSES
                     LACGEPGVVVGRDGRLSGPELAAALIRGLRESGCNVTDIGMVPTPVLYFATHVLGASS
                     GVMVTGSHNPPDYNGFKIVIAGHTLANERIQALHQRLENNDLASGSGSGSGSLTQVEV
                     LEQYHQRIADDVVLARPLKVVVDCGNGVAGVNAPQLIEALGCEVIPLFCDVDGTFPNH
                     HPDPGKPENLKDLIAKVAETGADLGLAFDGDGDRVGVVTNQGEMIYPDRLLMLFARDV
                     LSRNPGADVIFDVKCTRGLAGLISGYGGRPLMWKTGHSLIKAKMKETGALLAGEMSGH
                     IFFKERWYGFDDGIYSAVRLLEILSLEKRSAAEVFADFPVALSTPEINIKVTDERKFA
                     LIEALQRDAQWGDANLFTLDGVRVDYPHGWGLVRASNTTPVLVLRFEADDAAELARIQ
                     QVFHAQLKAVAPDLELPF"
     gene            1796..2701
                     /gene="argB"
                     /locus_tag="VIO83_06285"
     CDS             1796..2701
                     /gene="argB"
                     /locus_tag="VIO83_06285"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004575216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY8330914.1"
                     /translation="MPLNREAAEQVAKVLTEALPYIRRFAGKTLVVKYGGNAMESDEL
                     KTSFARDIVLMKAVGINPVVVHGGGPQIGDLLKRLSIESHFIDGMRVTDAQTMDVVEM
                     VLGGQVNKDIVNLINQHGGSAIGLTGKDAGLIRARKLKVTRQTPEMTQPEIIDIGHVG
                     EVVGVNVDLLNMLVAGDFIPVIAPIGVGANGESYNINADLVAGKVAEALKCEKLMLLT
                     NTAGLLDKQGQVLTGLSTAQVDGLIADGTIYGGMLPKIRCALEAVQGGVNSAHIIDGR
                     VPNAVLLEIFTDSGVGTLITNAQPR"
     gene            complement(2822..3409)
                     /locus_tag="VIO83_06290"
     CDS             complement(2822..3409)
                     /locus_tag="VIO83_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY8330915.1"
                     /translation="MQMGNKLLLLLLGVLAATPAQAELYRYLNRQGVVVLDRQGVPPE
                     YVGNGYEVIDEEGRVLRAVPPAPSAEELRQRVAAQRQARADAQLRRLYPRVADLEVAR
                     AHQLRDLDSLIVMARGNLNAVQQRVLGLEQQAAEQQRAGQPVPAALLQQLEGLRGEQR
                     QAEAELQRLQELRQRSETRFAADRLRLVEILGREP"
     gene            complement(3531..5006)
                     /locus_tag="VIO83_06295"
     CDS             complement(3531..5006)
                     /locus_tag="VIO83_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEY8330916.1"
                     /translation="MTDNNQKSYRELHKPKEQFASREEYLEHELTIMAPRRWALNLPF
                     RDYRFELEDWVPAMAATIGKIVMVAAVVAAFAGPLGLSSEFVIENARFEMLIAALLFV
                     LLFSGVFNPTANLAGTHGPLIPLIPLIVASGGHPMALGIMVGVLGFVLGISKGGSLLA
                     RLTSDGVCGGLLLYLGFIGVTSQIKSLFAWANGFDMGYVAFVIVFATIVMYAYLEHIR
                     MRWLAIPLGAILAAVIAFALGAPFEFSTEPGIPNLNPAYWWGEDTGWKLGLPEFQHFV
                     AVAPFAVLAVAMWSPDFLGHRIFQELNYPPKAKKVLMNIDDTMCIAAARQAVGTALGG
                     GNLTSSWGTYMVPAAIAKRPIPAGAILTGLLCVIAAVWGYPMDLAIWPPVLSVALLVG
                     VYLPLLEAGMQMTREGKTSQSAAIVIFSSALVNPVFGWSLTMLLDNLGLIGDKERGQG
                     LGHRDRWIIPGVVFLILCGVMAAIGMFPGIPALLESFRALA"
     gene            complement(5204..5845)
                     /gene="pyrE"
                     /locus_tag="VIO83_06300"
     CDS             complement(5204..5845)
                     /gene="pyrE"
                     /locus_tag="VIO83_06300"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY8330917.1"
                     /translation="MQAYQREFIRFAIERGVLRFGEFTLKSGRTSPYFFNAGLFNSGL
                     ALARLGRFYAEAVQASGIDFDVLFGPAYKGIPLASATAIALAEQHGRDLPWCFNRKEA
                     KEHGEGGSLVGAPLVGRALIVDDVITAGTAIREVMQIIQAQGAQAAGVLIALNREERG
                     QGELSAIQEVERDFGIPVVSIVSLSQVLDYLAQDQALKQHLPAVEAYRAQYGI"
     gene            5917..6696
                     /locus_tag="VIO83_06305"
     CDS             5917..6696
                     /locus_tag="VIO83_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY8330918.1"
                     /translation="MRIVSVNVNGMQAAVERGLLGWLQSQNADVICLQDTRASSYDME
                     DPAWQLDGYFMYAADAEVPSQGGVAIYSRQQPKAVIFGLGFEQADRYGRYLQADFDKV
                     SIASLLMPSGQKGDDDLNAKFKFMDDFTSYLNKQRRKRREYIYCSSLYVAHQKLDVKN
                     WRECQQAPGFLAPERAWMDEVLGNLGYVDALREVSREGDQYSWWPDTEQAAMLNLGFR
                     FDYQLLTPGLRRCVRSAQLPRQPRFSQHAPLVVDYDWTLSI"
     gene            complement(6756..7070)
                     /locus_tag="VIO83_06310"
     CDS             complement(6756..7070)
                     /locus_tag="VIO83_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEY8330919.1"
                     /translation="MLCHYAAFAWFLAPMIGNVIGPLVVWQLKRESDPYVDAQGKEAL
                     NFQITLSLALMVCGVLAWILIGFPLMVLVSVVGLVLTIIGGIKANEGKPWRYPFCLRL
                     LS"
     gene            complement(7184..7903)
                     /gene="rph"
                     /locus_tag="VIO83_06315"
     CDS             complement(7184..7903)
                     /gene="rph"
                     /locus_tag="VIO83_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY8330920.1"
                     /translation="MNRPSGRAADQMRPIRITRHYTKHAEGSVLVEFGDTKVICTVSA
                     EAGVPRFLKGQGQGWLTAEYGMLPRATGSRTQREAARGKQGGRTLEIQRLIGRSLRAA
                     LDLSKLGETTLYIDCDVIQADGGTRTASITGATVALIDALAALKKRGSLKQNPLKQLI
                     AAVSVGMYQGEPVLDLDYLEDSAAGTDLNVVMTDAGGFIEVQGTAEGTPFQPEELNAM
                     LELARKGITELFELQRAALDN"
     gene            8068..>8394
                     /locus_tag="VIO83_06320"
     CDS             8068..>8394
                     /locus_tag="VIO83_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY8330921.1"
                     /translation="MVHSMTAFARVENAGPYGTLSWELRSVNHRYLEPHLRLPEAFRD
                     LEGSVREALRQALARGKVECTLRLAEEGSGKSLQVDRERAAQVVAAAEAVAALIQQPA
                     QATDRGG"
BASE COUNT         1539 a         2913 c         2639 g         1303 t
ORIGIN      
        1 cgtggaccgg cctgctggct ggcctgctgg tgctggcggg ggccctcggc ggcgccgccc
       61 tgtaccggca tgccgaacgg caacgccagc ggcatgatcg cgaggccctg gagagctata
      121 cccggcgtct gggcatcagc ccgcaggcgc cggcgccgca gctgctcgac gacgaatacc
      181 gcgacctcgc cgacctgctg gccagactgc cgccgagcga accgcgcggc gttgccgcag
      241 tctcagtagc tagcgacctg aacactcccg cgcccgccgc cgcgccgacc ccggccccgg
      301 cggacgtctt cgacctgatt gatcccgaac cggccctgcc ggctcaaccc agcatggagc
      361 aaaacgccca gatgaatacc agcaccaacg ccgcgcacct gccggccggc atcttccgcg
      421 cctatgacat ccgcggcgtg gtcggcgaca gcctgaccag cgaaaccgcc tactggatcg
      481 gccgcaccat cggctcggaa agtctcgcct gcggcgagcc cggcgtggtg gtcggccgcg
      541 acggccgcct gtccggcccg gagctggccg cagcgctgat ccgcggcctg cgcgagagcg
      601 gctgcaacgt caccgacatc ggcatggtgc cgaccccggt gctgtacttc gccacccacg
      661 tgctcggcgc cagcagcggc gtgatggtca ccggcagcca caacccgccg gactacaacg
      721 gcttcaagat cgtcatcgcc ggccacaccc tggccaacga gcgcatccag gccctgcacc
      781 agcgcctgga gaacaacgac ctggccagcg gcagcggcag cggcagcggc agcctgaccc
      841 aggtcgaggt gctcgaacag taccaccagc gcatcgccga cgacgtggtg ctggcccgtc
      901 cgctcaaggt ggtggtcgac tgcggcaacg gcgtggccgg ggtcaacgcc ccgcagctga
      961 tcgaggccct cggctgcgag gtgatcccgc tgttctgcga cgtcgacggc accttcccca
     1021 accaccaccc ggacccgggt aagccggaga acctcaagga cctgatcgcc aaggtcgccg
     1081 agaccggcgc cgacctgggc ctggccttcg acggcgacgg cgaccgcgtc ggcgtggtga
     1141 ccaaccaggg cgagatgatc tatcccgacc gcctgctgat gctgttcgcc cgcgacgtgc
     1201 tgagccgcaa ccccggcgcc gacgtgatct tcgacgtcaa gtgcacccgc ggcctggccg
     1261 gcctgatcag cggctacggc ggtcgtccgc tgatgtggaa gaccggtcac tcgctgatca
     1321 aggcgaagat gaaggaaacc ggcgccctgc tggccggcga gatgagcggg cacatcttct
     1381 tcaaggagcg ctggtacggc ttcgacgacg gcatctacag cgccgtgcgc ctgctggaaa
     1441 tcctcagcct ggagaagcgc agcgccgccg aggtgttcgc cgacttcccg gtcgcgctgt
     1501 ccaccccgga aatcaacatc aaggtcactg acgagcgcaa gttcgccctg atcgaggccc
     1561 tgcagcgcga cgcgcagtgg ggcgacgcca acctgttcac cctcgacggc gtgcgcgtcg
     1621 actaccccca tggctggggc ctggtgcgcg cctccaacac cactccggtg ctggtgctgc
     1681 gcttcgaggc cgacgacgcc gccgaactcg cgcggatcca gcaggtcttc cacgcccagc
     1741 tcaaggccgt ggcccctgac ctggaactgc ccttctgatg ccactggagc ccagcatgcc
     1801 tctcaatcgt gaagctgccg aacaggtagc caaggtcctc accgaagccc tgccctacat
     1861 ccgccgcttc gccggcaaga ccctggtggt caagtacggc ggcaacgcca tggaaagcga
     1921 cgagctgaag accagcttcg cccgcgacat cgtgctgatg aaggccgtcg gcatcaaccc
     1981 ggtggtggtg cacggcggcg gcccgcagat cggcgacctg ctcaagcgcc tgtcgatcga
     2041 gagccacttc atcgacggca tgcgcgtcac cgacgcgcag accatggacg tggtggagat
     2101 ggtcctcggc ggccaggtca acaaggacat cgtcaacctg atcaaccagc atggcggcag
     2161 cgccatcggc ctgaccggca aggatgccgg gctgatccgc gccaggaagc tcaaggtgac
     2221 ccgccagacg ccggagatga cccagccgga gatcatcgac atcggccacg tcggcgaagt
     2281 cgtcggggtc aacgtcgacc tgctgaacat gctggtggcc ggcgacttca tcccggtgat
     2341 cgcgccgatc ggcgtgggcg cgaacggcga gtcgtacaac atcaacgccg acctggtggc
     2401 cggcaaggta gccgaggcgc tcaagtgcga gaagctgatg ctgctgacca acaccgcggg
     2461 cctgctggac aagcagggcc aggtgctgac cggcctgtcc accgcccagg tcgacggcct
     2521 gatcgccgac ggcaccatct acggcggcat gctgccgaag atccgctgcg ccctcgaggc
     2581 ggtgcagggc ggggtcaaca gcgcgcacat catcgacggc cgcgtgccga atgccgtgct
     2641 gctggagatc ttcaccgaca gcggcgtcgg caccctgatc accaacgcgc agccgcgctg
     2701 aggggtcacc aaggcccgcc agggctgagg gctcgccgag gcgctaccgc gccgcggcga
     2761 gcggtcgcgc cggaggtctc ctccggcgca ggccgttagc aggtggttgt tcaacaaccg
     2821 gtcagggctc gcgaccgaga atctcgacca gccgcagccg gtcggcggca aaccgcgtct
     2881 cgctgcgctg acgcagctcc tgcaggcgct gcagctcagc ctcggcttgc cgctgctcgc
     2941 cgcgcaagcc ctccaactgc tgcagcagcg ctgccggtac cggctggccg gcccgctgct
     3001 gctccgccgc ctgctgctcc agccccagca cccgctgctg caccgcgttc aggttgccgc
     3061 gcgccatcac gatcaagctg tccagatcgc gcaactgatg cgcccgcgcc acctcgaggt
     3121 cggccacgcg gggatagagc cggcgcagct gcgcatcggc gcgggcctga cgctgcgccg
     3181 cgacccgctg gcgcaactcc tcggcactcg gcgccggcgg caccgcgcgc agcacccggc
     3241 cctcttcgtc aatcacctcg tagccattgc cgacgtattc cggcggcacg ccctggcgat
     3301 ccagcaccac caccccctgc cggttcagat agcggtacag ctcggcctgg gccggggtcg
     3361 ccgccagcac ccccaacagc aacaacaaca gcttgtttcc catctgcatt gatcagcccc
     3421 tcgcgtcatc gggcttgctc gggcgacgct gagcgcctgt tcgcgacctg gtaattctcc
     3481 gacaaaaaag gggagccgca gctccccctc tttttgcccg cgctatgtta tcaggccagc
     3541 gcgcggaaag actccagcag tgccgggatg cccgggaaca tgccgatcgc cgccatcacc
     3601 ccgcagagga tcagaaacac cacgcccggg atgatccagc gatcgcgatg gcccagaccc
     3661 tggccgcgct ccttgtcgcc gatcaggccg aggttgtcga gcagcatggt cagcgaccag
     3721 ccgaacaccg ggttcaccag cgccgaggag aagatcacga tggccgccga ctgcgaggtc
     3781 ttgccctcgc gggtcatctg catgccggct tccagcagcg gcaggtacac accgaccagc
     3841 agggccacgc tgagcaccgg cggccagatc gccaggtcca tcgggtagcc ccacaccgca
     3901 gcgatcacgc agagcaggcc ggtgaggatc gcgccggccg gaatcggccg cttggcgatg
     3961 gccgccggca ccatgtaggt gccccaggac gaggtcaggt tgccgccgcc cagcgccgta
     4021 ccgaccgcct ggcgcgccgc ggcgatgcac atggtgtcgt cgatgttcat caggaccttc
     4081 ttggccttgg gcggatagtt cagctcctgg aagatccgat gaccgaggaa gtccggcgac
     4141 cacatcgcca ccgccagcac cgcaaagggc gccacggcaa cgaaatgctg gaattccggc
     4201 agacccagtt tccagccggt atcttcgccc caccagtagg ccggattcag attgggaata
     4261 cccggctcgg tgctgaactc gaacggcgcc cccagcgcga aggcaattac cgcggccaga
     4321 atggcaccca gcggaatcgc cagccagcgc atacgaatat gttccaggta ggcatacatc
     4381 acgatggtgg caaacacgat cacgaaggcg acatagccca tgtcgaagcc attggcccag
     4441 gcgaacaggc tcttgatctg cgaagtaacg ccaatgaagc cgagatagag cagcaggccg
     4501 ccacacaccc cgtcactggt cagccgcgcc agcagactgc cgcccttgga aatccccagc
     4561 acgaagccca gcacgccgac catgatgccc agcgccatcg gatggccgcc ggaggcgacg
     4621 atcagcggaa tcagcggaat cagcgggcca tgggtgccgg ccaggttggc agtcgggttg
     4681 aacaccccgg aaaacagcag cacgaacagc agcgcggcga tcagcatctc gaagcgggcg
     4741 ttttcgatga cgaactcgct ggacaggccc agcggcccgg caaacgcggc caccaccgcg
     4801 gcgaccatga caatcttgcc gatggtcgcg gccatcgccg ggacccagtc ttccagttcg
     4861 aagcgatagt cgcggaaggg caggttcagc gcccagcgcc gcggcgccat gatggtcagt
     4921 tcgtgctcga ggtattcttc ccgcgaggca aactgttctt tgggcttatg cagctcgcga
     4981 taactctttt gattattatc agtcatacat tcaactatta ttagattaat attaaagacg
     5041 aagtcaggcc gtgacctgac ttgctctgct taatcgctat tattcttatg aggcgggtga
     5101 ccatgctccg catggccacc tggcaggcct tgaactggta tgccaattca gaaaaatagt
     5161 tctttttaat agttgttata tggcctaaaa ctttttgcgc cgatcagatt ccgtactgcg
     5221 cgcggtaggc ttccactgcc ggcagatgct gcttgagcgc ctgatcctga gcgagataat
     5281 ccagcacttg gctcagcgag acaatgctta ccaccggtat accaaagtcg cgctcgactt
     5341 cctggatcgc cgaaagttca ccctggccac gctcctcgcg attcagcgca atcagcacgc
     5401 cggcggcctg cgcaccctgc gcctggatga tctgcatcac ctcgcggatc gccgtaccgg
     5461 cggtgatcac gtcatcgacg atcagcgccc gaccgaccaa cggcgcaccc accaggctgc
     5521 caccctcgcc atgttccttg gcttccttgc ggttgaagca ccacggcaga tcgcgaccat
     5581 gctgctcggc cagcgcgatg gcggtggcgg aagccagtgg gatgcccttg taggccgggc
     5641 cgaacagcac gtcgaagtcg atgccgctgg cctgcaccgc ttcggcgtag aaacggccga
     5701 gacgggcgag ggccaaaccg ctgttgaaca gcccggcatt gaagaagtag ggactggtgc
     5761 gcccggactt gagggtgaac tcgccgaagc gcagtacgcc gcgctcgatg gcgaagcgga
     5821 tgaattcgcg ttgatatgcc tgcatgggat gcctgtttta gccaaaaaag cacgaagctc
     5881 aggtatcata cacacaggtt ttttcagggg ccatttatgc ggattgtcag tgtcaacgtg
     5941 aacggtatgc aggcagcggt cgagcgcggc ctgctcggtt ggctgcaaag ccagaatgcc
     6001 gacgtcatct gcctgcagga cacccgcgcc tcgagctacg acatggaaga tcccgcctgg
     6061 cagctggacg gttacttcat gtatgccgcc gacgccgagg ttcccagcca gggcggggtg
     6121 gctatctatt cgcggcagca acccaaggcg gtgatcttcg gcctcggctt cgagcaggcc
     6181 gaccgttacg ggcgctacct gcaggccgac ttcgacaagg tcagcatcgc cagcctgctg
     6241 atgcccagcg ggcagaaagg cgacgacgac ctgaacgcca agttcaagtt catggacgac
     6301 ttcaccagct atctgaacaa gcagcgtcgc aagcgccgcg agtacatcta ctgcagctcg
     6361 ctgtacgtgg cgcaccagaa gctggacgtg aagaactggc gcgagtgcca gcaggcgccg
     6421 ggcttcctcg cacccgagcg ggcgtggatg gacgaagtgc tgggcaacct cggctacgtc
     6481 gatgccctgc gcgaagtcag ccgcgagggt gaccagtaca gctggtggcc ggacaccgag
     6541 caggcggcca tgctcaacct cggcttccgc ttcgactacc agctgctcac ccccggcctg
     6601 cgccgctgcg tacgcagcgc ccagctgccg cgccagccgc gcttctccca gcacgcaccg
     6661 ctggtggtcg actacgactg gacgctgagc atctgacgcc gcacgcatgc aaaaagccga
     6721 ctcagcgagt cggctttttt atgcaccgcg cgggctcagc tcagcagacg caggcagaac
     6781 ggatagcgcc agggcttgcc ctcgttggcc ttgatgccac cgatgatggt cagcaccagg
     6841 ccgaccacgc tgaccagcac catcagcgga aagccgatca ggatccaggc cagcacgcca
     6901 cagaccatca gcgccaggct cagggtgatc tggaagttca gcgcttcctt gccctgcgca
     6961 tcgacgtagg ggtcgctctc gcgcttgagc tgccagacca ccagcgggcc gatgacattg
     7021 ccgatcatcg gcgcaagaaa ccaggcgaag gccgcatagt ggcagagcat cgcccacttg
     7081 cgcgcctcgg cggaggggga tggcaccggc agttgcccct cgggccgccg ctcatcggac
     7141 tgcatctgct cgctcatcgc tcacctccgg ccagcggggc cgttcagttg tccagcgccg
     7201 cgcgctgcag ttcgaacagc tcggtgatgc ccttgcgcgc cagttcgagc atcgcattga
     7261 gctcttccgg ctggaacggc gtgccctcgg cggtgccctg cacctcgatg aagccaccgg
     7321 cgtcggtcat caccacgttg aggtcggtac cggccgccga atcctccaga tagtcgagat
     7381 cgagcaccgg ttcgccctgg tacatgccca ccgacaccgc cgcgatcagt tgcttgagcg
     7441 ggttctgctt cagcgaacca cgcttcttca gcgccgccag cgcatcgatc agcgccacgg
     7501 tggcgccggt gatcgacgcg gtgcgggtgc cgccgtcggc ctggatcacg tcgcagtcga
     7561 tgtacagggt ggtctcgccg agcttgctca ggtccagtgc ggcacgcagc gagcggccga
     7621 tcaggcgctg gatctccagg gtgcggccgc cctgcttgcc gcgcgccgct tcacgctggg
     7681 tacgcgagcc ggtggcgcgc ggcagcatgc cgtattccgc ggtcagccag ccctggccct
     7741 ggcccttgag gaagcgcggc acgccggctt cggcactcac cgtgcagatc accttggtgt
     7801 cgccgaactc caccagcacc gaaccctcgg catgcttggt gtagtggcgg gtgatacgga
     7861 tcgggcgcat ctggtcggcg gcgcgaccac tgggacgatt catcggcatg tcctgtcgag
     7921 agaagaaaaa ctgcgcccca ttatagaggt ccctgcgcat cgcgaaccac cgtcgcattg
     7981 gggccgcccg gcgcctgggt tacactggcg gactgaatac gtacggcgtc catgcgcgcc
     8041 atccccttat cactgcgagg accgcccatg gtacacagca tgaccgcctt tgcccgggtc
     8101 gagaacgccg ggccgtacgg caccctgagc tgggagctgc gctcggtcaa ccaccgctac
     8161 ctggagccgc acctgcgcct gccggaagcc ttccgcgatc tcgagggcag cgtccgcgaa
     8221 gcgctgcgcc aggccctggc ccgcggcaag gtcgaatgca ccctgcgcct ggccgaggaa
     8281 ggcagcggca agtcgctgca ggtcgatcgc gagcgcgccg cccaggtggt cgccgccgcc
     8341 gaagcggtcg ccgcgctgat ccagcagccc gctcaagcaa ctgatcgcgg cggt
//
LOCUS       DATCUN010000156        10236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_213202, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000156 DATCUN010000000
VERSION     DATCUN010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10236
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213202"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VIO83_06325"
     CDS             <1..664
                     /locus_tag="VIO83_06325"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8330922.1"
                     /translation="KQLAAFGRPRKLDSARHCQLWQVTVDNAPAVPDLDALARRYPVA
                     TPQGELQVVSLPGVFSHGRLDVGSRLLLEHLDGLPQGELLDFGCGAGVLGAALQRRYP
                     HSHLHLLDVDAFAVASSRQTLAANGLQGQVLAGSGIADAPHELAAIVSNPPFHQGVHT
                     DYQATEQLLREAARHLRPGGELRLVANSFLKYAPLIEKHLGPCTVLAERDGFRVYSAR
                     RN"
     regulatory      856..1032
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            1065..1649
                     /locus_tag="VIO83_06330"
     CDS             1065..1649
                     /locus_tag="VIO83_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEY8330923.1"
                     /translation="MQSLFVPTGLVALAEIGDKTQLLALLLAARFRKPWPIIWGILVA
                     TVANHFAAGALGKWVASFFSEATLSWVLAASFLAVAAWMLVPDQIDEDEASHFKRYGP
                     FLTTLIAFFLAEMGDKTQIATVMLAAQYEHFWLVVTGTTLGMLIANVPVVLAAHFSAD
                     KLPMTLIHRISALCFLALAGYAAWHAATLSGWLG"
     gene            1832..3571
                     /gene="dauA"
                     /locus_tag="VIO83_06335"
     CDS             1832..3571
                     /gene="dauA"
                     /locus_tag="VIO83_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HEY8330924.1"
                     /translation="MSRLARVPLFTAWRQTLRAGYTRQALRGDILAGLTVGIIAIPLA
                     MALAIAVGAPPQHGLYTVLVAAPLIAISGGSRFNVSGPTAAFVVILLPIVQQYGLAGL
                     LLCSMLAGLILIALGLLKAGRLIQFVPYPVTLGFTAGIGVVIGGLQLKDALGLTVTAS
                     AAHFPEQMLNLVLALPSAHLGDALVAIVTLAVLIAWPRLVPRVPGHLAALAVGTLFGL
                     LLEFAGLGIATLGERFSYMVDGVAQPGIPPYLPSFALPWQLADAPPLSLALIRELLAP
                     AMAIAMLGAIESLLCAVVADGMSGSKHDSNGELIGQGLGNLIAPLFGGITATAAIART
                     AASLRAGAFSPLAAVIHAGVVLAAMLLLAPLFSYLPMAALAALLLMVAWNMAEAPHVV
                     HTLRIAPRSDVVVLLTCLVLTVAFDMVLAVGAGLLLAAGLFIRQMSALTDTAPLPRHF
                     HQALHDLPEHVLAYAIRGPLFFGAAEKALNTLCRFSPGLKVVIVDISAVPLLDMTALA
                     ALETVLRDFRHHGVGLIFSGTSSRVRLQLRRAGIHRHNHQLAYVHDLDQARGKALRWL
                     EEKAEPAVTAASA"
     gene            complement(3695..4252)
                     /locus_tag="VIO83_06340"
     CDS             complement(3695..4252)
                     /locus_tag="VIO83_06340"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEY8330925.1"
                     /translation="MSVELAHIRQVMAEADCLYTADQLDAAIARLAGEINRDLAERNP
                     LVFCVMNGGLIFAGKLLALLDFPLELSYLHATRYRGETSGGELFWKAKPEMSLIDRDV
                     LIVDDIFDEGHTLAAIVDYCKHAGASAVHTAVLIDKEHDRKARPDMNASYVGLPCVDR
                     YIFGYGMDYKGYWRNAPGIFAVKGM"
     gene            4410..5048
                     /gene="upp"
                     /locus_tag="VIO83_06345"
     CDS             4410..5048
                     /gene="upp"
                     /locus_tag="VIO83_06345"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY8330926.1"
                     /translation="MPVHEIRHPLIRHKLGLMRRADISTKNFRELAQELGALLTYEAT
                     QDLPLEDEYIQGWCGEVQVERIAGKKITVVPILRAGIGMLDGVLSLIPSAKVSVVGLA
                     RNEATLEAERYLEKLVPGIEQRLALIVDPMLATGGSLIATIDLLKAAGCREIRALVLV
                     AAPEGIAALSAAHPDVQVYAAAIDQRLNEHGYIIPGLGDAGDKIFGTRQKDA"
     gene            5229..6212
                     /locus_tag="VIO83_06350"
     CDS             5229..6212
                     /locus_tag="VIO83_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HEY8330927.1"
                     /translation="MSSHVAKPKMKRSDLLWTLIGLGAVVLSCFLLYRELHSVSLDEV
                     AGSLRAIPGLNWLLAGGATLGAYFALAWYDHIALAHLGKRVPWRFITLCSFTTYALAH
                     NIGASVFSGAVVRYRAYRSKGLSPQEIGLLIFFCSFTFGLGTLLASGCVLVLRPGLTQ
                     QILNVPPALSVAIGLLLLLLVGLYVLGSWRQFKPWTRGKLHVEYPRLGIVARQLLAGP
                     LELLCAAAIIYFALPAAHNPGYLVVLGVFLASFSLALLSHAPGGLGVLEMSFIAALPG
                     IPTVDVLAALIVFRALYLLLPFALSLLVVLGFEWGQWRRHKAGDAGPRPPA"
     gene            6281..7546
                     /locus_tag="VIO83_06355"
     CDS             6281..7546
                     /locus_tag="VIO83_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY8330928.1"
                     /translation="MTLASPARACGIDFGTSNSTVGWRRPGADNLIALEDGKDTLPSV
                     LFFNFEEQRPAFGRQALGEYLDGYEGRLMRSLKSLLGSPLLGSATTVLGSAMPFKQIL
                     GLFLGELKQRAEAAAGRPFEQVVLGRPVFFVDDDPAADREAADTLVEVARELGFQDVS
                     FQYEPIAAAFDYESGIDREELVLIVDIGGGTSDFSLVRLSPERRALAERQGDILATGG
                     VHIGGTDFDKQLSLNGVMPLFGYGSRMKSDALMPTSHHMNLATWHTINAVYAQKSKIA
                     LESMRYDIADPTGIDRLLRLIEQRAGHWLAIEVEASKIALTEEQRRHIDFSRIEAGLS
                     AELTRALFEAAIAQQLERINAGIGQLLSDAGVTAEQVDTLFFTGGSSGIPTLRQSAAR
                     LLPNARQVEGNRFGGIGSGLAIEAHKRYG"
     gene            7759..>10236
                     /locus_tag="VIO83_06360"
     CDS             7759..>10236
                     /locus_tag="VIO83_06360"
                     /inference="COORDINATES: protein motif:HMM:NF019874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8330929.1"
                     /translation="MTESNLLRLQLFGMLGLVFVLTLALGGYFTLQQTRSFREDSAAL
                     EAAGRREQKAMLAASLTTLHNQLELLRQSSEAVLKRDIRQQVDHAVQIATALYQQEKG
                     RRPEGEIRRLIVETLRPLRFFGGSGYFFIDDLAGNCVLLPINPEREGHSLLDNRDVDG
                     RYIMRDLLASVANPQQRGFSRYRWYAPGNPAAMADKIAYARVFEPFGWLIGSGEYLSK
                     IEHLLQQQALRQLRTTRLGSNGYIAVIHRDGRVLLSPSNPAAEGRRVDELPPAEQAAI
                     RNILDTSHNGGGFTLYQWRSLVEPRLSAKLSLVSNLDEWGWILVSGVYMDDLQQSLAQ
                     RQLKLEQDLQASLQTTVLALLLVMALALGLAAGLSRWLSRRFARYQQDIDAGHERLRV
                     WAESDPLTLLPNRACLRSRLDEAIARAHGDGLQLALLFIDLDRFKNINDSLGHAIGDG
                     LLVQVAQRMSVALRVSDTVSRLGGDEFVVLLEGLSHSEQAAQVAALLLHATSGQYSVA
                     GHDLAITLSIGIAVYPADGADAETLLKNADAAMYHAKACGRNNFQFFAAAMNTQVREH
                     LELENRLRQALARHELSLHYQPQYDLQSGRLSGCEALMRWHNPELGQVAPDKFIPIAE
                     DSGLILPLGEWALGEACHQVMAWQAQGLPLLSVAVNVSAVQFRHADLAAMVERTLRSS
                     GLPPALLELELTESVLAENLEQVNATLQRLKQLGVRLAMDDFGTGYSSLAYLKHFKLD
                     TLKIDRSFVSDLPGSSDNAALAITIIGMARSLGMTCIAEGIETVAQHDFLRGHGCTHG
                     QGYRLSRPLTSAAMAALLGASRGDAETP"
BASE COUNT         1523 a         3830 c         3293 g         1590 t
ORIGIN      
        1 caagcagctg gccgccttcg gccgaccgcg caagctggac agcgcgcgcc actgccagct
       61 gtggcaggtg acggtggaca acgccccggc cgtaccggac ctcgacgccc tcgcccgccg
      121 ctacccggtg gcgaccccgc agggtgaact gcaggtggtc agcctgcccg gcgtgttcag
      181 ccacggccgc ctggacgtcg gttcgcgcct gctgctggag cacctcgacg gcctgccgca
      241 gggcgagctg ctcgacttcg gctgcggcgc cggggtgctc ggcgccgcgt tgcagcgccg
      301 ctatccgcac agccacctgc acctgctgga tgtcgacgcc ttcgccgtcg ccagcagccg
      361 ccagaccctc gccgccaacg gcctgcaagg ccaggtgctg gccggcagcg gcatcgccga
      421 tgcgccgcac gagctggccg ccatcgtcag caacccgccg ttccaccagg gcgtgcacac
      481 cgactaccag gccaccgagc agctgctgcg cgaggccgcc cgccacctgc gccccggcgg
      541 cgagctgcgc ctggtggcca acagcttcct caagtacgcg ccgctgatcg aaaagcatct
      601 cggcccctgc acggtgctgg ccgagcgcga cggcttccgc gtctattcgg cgcggcgcaa
      661 ctgatctcct gtagggtgga caacggcgca gccttgtcca ccacccagca acgcaatccg
      721 taggctggaa aactcgcgca gcgattttcc gccagcccct gtatccatcc cggcgccaac
      781 ggtggaaaag acctgcggtc gttttccacc ctacgccttg cctgccccat cccgcttggg
      841 cacaatgcgc gccgtcctga gggagtagtc tgccgccgag cgattcggcg gcgtgcgcgt
      901 caacatactt ggtccacaga ccatggcgcg cacaacccgc accggccacg ccggcgccgc
      961 ggttcgacaa gactcatgac actcacaagc cgacccgggg cgggcgagct gtgcgtgtca
     1021 tgtcattgcc gacccgcccc ccactggaat gcctgatgga atacctgcaa tcgctgttcg
     1081 tccccaccgg cctggtcgcc ctcgccgaaa tcggcgacaa gacgcagctg ctcgccctgc
     1141 tgctcgccgc ccgtttccgc aagccctggc cgatcatctg gggcatcctg gtcgccaccg
     1201 tcgccaacca cttcgccgcc ggcgcgctgg gcaagtgggt cgcctcgttc ttcagcgaag
     1261 ccaccctgag ctgggtgctc gccgcctcct tcctagccgt ggccgcctgg atgctggtgc
     1321 ccgaccagat cgacgaggac gaagcctcgc acttcaagcg ctacgggccg ttcctcacca
     1381 cgctgatcgc cttcttcctc gccgagatgg gcgacaagac gcagatcgcc acggtgatgc
     1441 tggccgcgca gtacgagcat ttctggctgg tggtcaccgg caccaccctg ggcatgttga
     1501 tcgccaacgt gccggtggta ctggccgcgc acttctcggc cgacaagctg ccgatgaccc
     1561 tgatccaccg catctccgcg ctgtgcttcc tggcactggc cggctatgcc gcctggcatg
     1621 cggcgaccct gagcggctgg ctgggctgag gcgcgccatg accgtgtagg agcgcgcttg
     1681 ctcgcgatca tgctgcgcgc aggcatacgg atcgccagca agccggctcc tgccgggaat
     1741 cgcttcgccg ttccacctcc gtcccatagc cgtttgtcgg taaacggctt aacctctcag
     1801 agaagagggg aaatataatg gaggacaagc aatgtcccgg cttgcgcgcg ttccgctgtt
     1861 cactgcctgg cgccagaccc tgcgcgccgg ctacacccgt caggcgctgc gcggcgacat
     1921 cctcgccggg ctgacggtcg gcatcatcgc catcccgctg gccatggccc tggccatcgc
     1981 cgtcggcgcc ccgccgcagc acggcctgta caccgtgctg gtggccgcgc cgctgatcgc
     2041 catcagcggc ggctcgcgct tcaacgtctc cggtcccacc gccgccttcg tggtgatcct
     2101 gctgcccatc gtccagcagt acggcctcgc cggcctgctg ctgtgcagca tgctcgccgg
     2161 cctgatcctg atcgccctcg gcctgctcaa ggccggccgc ctgatccagt tcgtgcccta
     2221 tccggtcacc ctcggcttca ccgccggcat cggcgtggtc atcggcggcc tgcagctcaa
     2281 ggacgccctc ggcctgacgg tgaccgccag cgcggcgcac ttccccgagc agatgctcaa
     2341 cctggtgctc gccctgccca gcgcccatct gggcgatgcg ctggtcgcca tcgtcaccct
     2401 cgccgtgctg atcgcctggc cgcggctggt gccgcgggtg cccggccatc tcgccgcgct
     2461 ggcggtgggc accctgttcg gcctgctgct ggagttcgcc ggcctgggca tcgccaccct
     2521 cggcgagcgc ttcagctata tggtcgacgg cgtcgcgcag ccgggcatcc cgccctacct
     2581 gccgagcttc gccctgccct ggcagctggc cgacgccccg ccgctgagcc tcgcgctgat
     2641 ccgcgagctg ctcgccccgg cgatggccat cgccatgctc ggcgccatcg aatccttgct
     2701 ctgcgcggtg gtggccgacg gcatgagcgg cagcaagcac gactccaacg gcgagctgat
     2761 cggccagggg ctgggcaacc tgatcgcccc gctgttcggc ggcatcaccg ccaccgcggc
     2821 gatcgcccgc accgccgcca gcctgcgcgc cggcgccttc tcgccgctgg cggcggtcat
     2881 ccatgccggc gtggtgctgg ccgccatgct gctgctggcg ccgctgttca gctacctgcc
     2941 gatggcggcg ctggctgcgc tgctgctgat ggtggcgtgg aacatggccg aggcgccgca
     3001 cgtggtgcat accctgcgca tcgccccgcg cagcgacgtg gtggtgctgc tgacctgcct
     3061 ggtgctgacc gtggcgttcg acatggtgct ggcggtgggc gccggcctgc tgctggccgc
     3121 cggcctgttc atccgccaga tgagcgcgct gaccgacacc gcgccgctgc cgcgccactt
     3181 ccaccaggcg ctgcacgacc tgcccgagca tgtgctggcc tacgcgatcc gcggcccgct
     3241 gttcttcggc gcggcggaaa aggcgctgaa caccctgtgc cgcttcagcc cgggattgaa
     3301 ggtggtgatc gtcgatatca gcgcagtgcc gctgttggac atgaccgccc tggcggcgct
     3361 ggaaaccgtg ctgcgcgact tccgccacca cggcgtcggc ctgatcttca gcggcaccag
     3421 ctcgcgggtg cgcctgcagc tgcgccgcgc cggcatccac cgccacaacc accagctggc
     3481 ctacgtgcac gacctggatc aggcgcgcgg caaggcgctg cgctggctgg aggagaaggc
     3541 ggagccggcg gtcaccgccg cgtccgcgta gggtgggtca ggctgcaggc cgtaacccac
     3601 caaccaggcc gcaggcctgg cattcgcatg cgcgacctcg cggtcgcgat ggtgggttac
     3661 gccgctgcgc ggctaaccca ccctaccaag ggcctcacat gcccttgacc gcgaagatcc
     3721 ccggcgcgtt gcgccagtag cccttgtagt ccatgccgta gccgaagatg tagcggtcga
     3781 cgcagggcag gccgacgtag ctggcgttca tgtccgggcg cgccttgcgg tcgtgctcct
     3841 tgtcgatcag cacggcggtg tgcaccgcgc tggcgccggc gtgcttgcag tagtcgacga
     3901 tggcggccag ggtgtggccc tcgtcgaaga tgtcgtcgac gatcagcacg tcgcggtcga
     3961 tcagcgacat ctccggcttg gccttccaga acagctcgcc gccgctggtc tcgccgcggt
     4021 agcgggtggc gtgcaggtag gacagctcca gcgggaagtc cagcagcgcc agcagcttgc
     4081 cggcgaagat cagcccgccg ttcatcacgc agaacaccag cggattgcgc tcggccaggt
     4141 cgcgattgat ctcgccggcg aggcgcgcga tggcggcgtc cagctggtcg gcggtgtaca
     4201 ggcagtcggc ttccgccatc acctggcgga tgtgcgcgag ttcgacggac atggcggctt
     4261 ctctcccggg ttggccgcgg cgtgcagcgc ggcaaaggcc agcaaggtac ccatccgccc
     4321 gcccggcggc aagcctgcca atggcgctgc atacctggcg ggccgctgga tgctttactt
     4381 gccgtcacca ccttcaggga gatccgccca tgcccgtcca cgagatccgc cacccgctga
     4441 tccgccacaa gctcggcctg atgcgccgcg ccgacatcag cacgaagaac ttccgcgaac
     4501 tggcccagga gctgggcgcc ctgctcacct acgaggcgac ccaggacctg ccgctggagg
     4561 acgagtacat ccagggctgg tgcggcgagg tgcaggtgga gcgcatcgcc ggcaagaaga
     4621 tcaccgtggt gccgatcctg cgcgccggca tcggcatgct cgacggcgtg ctcagcctga
     4681 tccccagcgc caaggtcagc gtggtcggac tggcgcgcaa cgaggcgacc ctcgaggccg
     4741 agcgctacct ggagaagctg gtgccgggga tcgagcagcg cctggcgctg atcgtcgatc
     4801 cgatgctggc caccggcggc tcgctgatcg ccaccatcga cctgctcaag gcggccggct
     4861 gccgggaaat ccgcgccctg gtgctggtcg ccgcaccgga aggcatcgcc gcgctgagcg
     4921 ccgcccaccc cgacgtgcag gtctatgccg ccgccatcga ccagcgcctc aacgagcacg
     4981 gctacatcat cccgggcctc ggcgatgccg gcgacaagat cttcggcacc cggcagaagg
     5041 acgcctgaag cgtctgccgc ggcgcaggtt acccatgcgg caacgctgca cgttgccgca
     5101 ccgggttgag cgtgggggtt cgcctcgggg atactgcgcc gcctgcacgg ccgccagcgg
     5161 ccgaaccagc agccggccca ggctcgcgca ccgtcgcgcc cagctaccca gtcaacgagg
     5221 aactgccgtt gtccagccac gtcgccaaac cgaagatgaa acgcagcgac ctgctgtgga
     5281 cgctgattgg tctgggtgcc gtggtgctgt cctgcttctt gctctatcgc gaactgcaca
     5341 gcgtctccct cgacgaggtg gccggcagcc tgcgggccat ccccgggctg aactggctgc
     5401 tggccggcgg cgccaccctg ggcgcctact ttgccctggc ctggtacgac cacatcgccc
     5461 tcgcccatct gggcaagcgg gttccctggc gcttcatcac cctctgttcg ttcaccacct
     5521 acgccctggc ccacaacatc ggcgcctcgg tgttctccgg cgcggtggtg cgctatcgcg
     5581 cctaccgcag caagggcctc agtccgcagg aaatcggcct gctgatcttc ttctgctcct
     5641 tcacgttcgg cctcggcacc ctgctcgcca gcggctgcgt gctggtgctg cgccccggcc
     5701 tgacccagca gatcctcaac gtcccgccgg cgctctcggt ggccatcggc ctgctgctgt
     5761 tgctgctggt cggcctgtat gtgctcggct cctggcgcca gttcaagccc tggacccgcg
     5821 gcaagctgca tgtcgagtac ccgcgtctgg gcatcgtcgc ccgccagctg ctggccggcc
     5881 cgctggagct gctgtgcgcc gcggcgatca tctacttcgc cctgccggcc gcgcacaacc
     5941 ccggctatct ggtggtgctc ggcgtgttcc tcgcctcctt ctcactggcc ctgctgtccc
     6001 acgccccggg cgggctgggc gtgctggaga tgtcgttcat cgccgcgttg ccgggcattc
     6061 cgaccgtcga cgtgctcgcc gccctgatcg tgttccgcgc cctctacctg ctgctgccgt
     6121 tcgccctgtc gctgctggtg gtgctcggtt tcgagtgggg ccagtggcgc cgtcacaagg
     6181 ccggcgacgc cggtccgcga ccgccggcct gatcgccggg gcagcattcc ccgccgtact
     6241 cgcatagaat gcgcggccgt ttttccggag ccgccttccc atgaccctcg cctcgcccgc
     6301 ccgcgcctgc ggcatcgact tcggcacctc caactccacc gtcggctggc gacgccccgg
     6361 cgccgacaac ctgatcgccc tcgaagacgg caaggacacc ctgccctcgg tgctgttctt
     6421 caacttcgag gagcagcgcc cggccttcgg ccgccaggcg ctcggtgaat acctcgacgg
     6481 ctacgaaggc cgcctgatgc gctcgctgaa gagcctgctg ggctcgccgc tgctgggcag
     6541 cgccaccacc gtgctgggca gcgccatgcc gttcaagcag atcctcggcc tgttcctcgg
     6601 cgagctgaag cagcgcgccg aagccgccgc cggccgcccc ttcgagcagg tggtgctggg
     6661 ccgcccggtg ttcttcgtcg acgacgaccc ggccgccgac cgcgaggccg ccgacaccct
     6721 ggtcgaggtc gcccgcgaac tcggcttcca ggacgtgtcc ttccagtacg agccgatcgc
     6781 cgcggccttc gactacgagt cgggcatcga ccgcgaggag ctggtgctga tcgtcgacat
     6841 cggcggcggc acctcggact tctcgctggt ccgcctgtcc cccgagcgcc gcgcgctggc
     6901 cgagcgccag ggcgacatcc tcgccaccgg cggcgtgcac atcggcggca ccgacttcga
     6961 caagcagctc agcctcaacg gcgtgatgcc gctgttcggc tacggcagcc ggatgaagag
     7021 cgacgcgctg atgcccacca gccaccacat gaacctggcc acctggcaca ccatcaacgc
     7081 ggtctacgcg cagaagtcga agatcgccct ggaaagcatg cgctacgaca tcgccgaccc
     7141 gaccggcatc gaccgcctgc tgcgcctgat cgagcagcgc gccggccact ggctggcgat
     7201 cgaggtggag gccagcaaga tcgccctcac cgaggagcag cgccgccaca tcgacttcag
     7261 ccgcatcgag gcggggctga gcgccgagct gacccgcgcg ctgttcgaag cggccatcgc
     7321 ccagcagctc gagcgcatca acgccggcat cggccagctg ctcagcgacg ccggcgtgac
     7381 ggccgagcag gtcgacacgc tgttcttcac cggtggctcc agcggcatcc cgacgctgcg
     7441 ccagagcgcg gcccgcctgc tgcccaacgc ccgccaggtc gagggcaacc gtttcggcgg
     7501 catcggcagc ggcctggcca tcgaggcgca caagcgctac ggctgacagc cggcccacga
     7561 ccggctgcgc gacgatcgag cgctgggcta aaggccccgc gccctctaga atgtgacgcc
     7621 gaccacaaaa acacctgcca ttcatcggga acggcaggtt agggtggaaa gttccccgaa
     7681 cgccagctac gcgccatcag cccgcgctca tccaggaaca cggccccatc tcgagcaagg
     7741 gaagtgcgga gcatggcgat gactgaaagc aacctcctgc gcctgcagct gttcggcatg
     7801 ctggggctgg tattcgtgct gaccctggcc ctgggcggct acttcaccct gcagcagacc
     7861 cgcagcttcc gcgaggacag cgccgccctc gaggccgcgg ggcgccgcga gcagaaggcc
     7921 atgctcgcgg catcgctgac caccctgcac aaccagctcg agctgctgcg ccagagcagc
     7981 gaggcggtgc tcaagcgcga catccgccag caggtcgacc acgccgtgca gatcgccaca
     8041 gcgctctacc agcaggaaaa gggccgccgc cccgaaggcg agatccgccg gctgatcgtc
     8101 gaaaccctgc gcccgctgcg cttcttcggc ggcagcggct acttcttcat cgacgacctc
     8161 gccggcaact gcgtgctgct gccgatcaat cccgagcgcg agggccactc gctgctggac
     8221 aaccgcgacg tcgacggccg ctacatcatg cgcgacctgc tggccagcgt cgccaacccg
     8281 cagcagcgcg gcttcagccg ctaccgctgg tacgcgccgg gcaaccccgc ggccatggcg
     8341 gacaagatcg cctatgcccg ggtcttcgag ccgttcggct ggctgatcgg cagcggcgaa
     8401 tacctgtcca agatcgagca cctgctgcag cagcaggccc tgcggcagct gcgcaccacg
     8461 cgcctgggca gcaatggcta catcgccgtg atccaccgcg acggccgggt gctgctgtcg
     8521 ccgagcaacc cggccgccga gggccgccgg gtcgacgaac tgccgccggc cgagcaggcc
     8581 gccatccgca acatcctcga caccagccac aacggcggcg gcttcaccct ctaccagtgg
     8641 cgcagcctgg tcgagccgcg gctgagcgcc aagctgtcgc tggtcagcaa cctggatgaa
     8701 tggggctgga tcctggtcag cggcgtgtac atggacgacc tgcagcaaag cctggcgcaa
     8761 cgccagctca agctcgagca ggacctgcag gccagcctgc agaccaccgt gctcgccctc
     8821 ctactggtga tggcgctggc cctgggcctg gccgccgggc tgtcgcgctg gctgtcgcgc
     8881 cgcttcgccc gctaccagca ggacatcgac gccggccacg agcgcctgcg cgtgtgggcc
     8941 gagtccgatc cgctgaccct gctgcccaat cgcgcctgcc tgcgcagccg cctcgacgag
     9001 gccatcgcgc gggcccacgg cgacggcctg cagctggccc tgctgttcat cgacctcgac
     9061 cgcttcaaga acatcaacga ctcgctcggc cacgccatcg gcgatggcct gctggtccag
     9121 gtcgcccagc gcatgtccgt ggccctgcgc gtcagcgaca ccgtcagccg cctcggcggc
     9181 gacgagttcg tcgtcctcct cgagggcctc agccacagcg aacaggccgc ccaggtcgcc
     9241 gcgctgctgc tgcacgccac cagcggccag tacagcgtcg ccggccatga cctggcgatc
     9301 accctgagca tcggcatcgc cgtctacccg gccgacggcg cggacgcgga aaccctgctg
     9361 aagaacgccg acgccgccat gtaccacgcc aaggcctgtg ggcggaacaa cttccagttc
     9421 ttcgccgcgg cgatgaatac ccaggtacgc gagcatctgg aactggaaaa ccgcctgcgc
     9481 caggcactcg cacgccacga actgagcctg cactaccagc cgcagtacga tctgcaaagc
     9541 ggccggctga gcggctgcga ggcgctgatg cgctggcaca acccggaact cggccaggtc
     9601 gcgccggaca agttcatccc catcgccgag gacagcggac tgatcctgcc gctgggcgag
     9661 tgggcgctcg gcgaggcctg ccaccaggtc atggcctggc aggcccaggg cctgccgctg
     9721 ctcagcgtgg cggtcaacgt gtcggcggtg cagttccgcc atgccgacct ggccgcgatg
     9781 gtcgagcgca ccctgcgcag cagcggcctg ccgccggcgc tgctggagct ggaactgacc
     9841 gaaagcgtgc tggcggagaa cctcgagcag gtcaacgcca ccctgcaacg cctcaagcag
     9901 ctgggcgtgc gcctggcgat ggacgacttc ggcaccggct actcgagcct ggcctatctc
     9961 aagcacttca agctggatac gctgaagatc gatcgctcgt tcgtcagcga cctgccgggc
    10021 tccagcgaca atgccgcgct ggccatcacc atcatcggca tggcccgctc actgggcatg
    10081 acctgcatcg ccgagggcat cgaaaccgtg gcccagcacg actttctgcg cggccacggc
    10141 tgcacccacg gccagggtta tcggctgtcc aggccgctga ccagcgcggc catggccgcg
    10201 ctgctgggcg ccagccgcgg cgacgccgag acgccc
//
LOCUS       DATCUN010000157         5978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_119222, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000157 DATCUN010000000
VERSION     DATCUN010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119222"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VIO83_06365"
     CDS             <1..411
                     /locus_tag="VIO83_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HEY8330930.1"
                     /translation="IDTRGIPAISALVEQASGRQVAWQKSVVGAGVFTHEAGIHVDGL
                     LKHRRNYEGLNPDELGRSHSLVLGKHSGAHMVRSRYRELGIELDDWQCQALLGRIRVF
                     STGSKRSPQAAELEEFYRQLGEQGAPVLAAGGRA"
     gene            408..1199
                     /gene="cysE"
                     /locus_tag="VIO83_06370"
     CDS             408..1199
                     /gene="cysE"
                     /locus_tag="VIO83_06370"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEY8330931.1"
                     /translation="MSLFGEWRDDIRCVFQRDPAARTRFEVLTTYPGVHAILLYRLAH
                     RLWRAEWRYPARLLSFLARLVSNVDIHPGAVIGPRFFIDHGAGVVIGETAEIGADVTL
                     YHGVTLGGTSWRKGKRHPTLGDGVLVGAGAKILGPISIGAGARVGANSVVVQDVPEGC
                     TVVGIPGKVVRLREAGQPNPYGIDLDHHLIPDPVGKAIACLLERLDSLEKQVEAGGRV
                     AAGSQQQYYQGCSVDNSICESDCAGGAVALGQALPLRLAASPAGD"
     gene            1225..1764
                     /locus_tag="VIO83_06375"
     CDS             1225..1764
                     /locus_tag="VIO83_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330932.1"
                     /translation="MDLQDFNGDGLYFDEAQSAAVDSLLAAAAEQYGEGTAEQPLLAA
                     QALAPDDLSVLVGLYRFYYYQHRYQDALQIAERVLAVVGPRLKLPARWQALDASHLAI
                     AAGRGIGLLRFHLLALKGAGYLSLRLGLFEQGKAMLAKVVELDADNRLGARLLLDVLA
                     ANNAEIVTFPTAATAETRP"
     gene            1761..2108
                     /locus_tag="VIO83_06380"
     CDS             1761..2108
                     /locus_tag="VIO83_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase-stabilizing/protective protein NifW"
                     /protein_id="HEY8330933.1"
                     /translation="MTVQGFSPDSDLTLDEAMEELVSAEDFLDFFAVPFAQGVVHINR
                     LHIMQRYHDYLTKAGSLDAHDDVARAAIYRQLLARAYQDFVDSDAQTEKVFKVFRMHE
                     PQTTFVSIDQLLS"
     gene            2127..2597
                     /locus_tag="VIO83_06385"
     CDS             2127..2597
                     /locus_tag="VIO83_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039800894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HEY8330934.1"
                     /translation="MSLPQFEYGDEVRVVRNVRNDGTYPGMDTGTLLIRRGSVGCVYD
                     VGTYLQDQLIYRVHFLESGRTVGCREEELIPASAPWTPSLFEFRDNVTAMRSFAVRGE
                     VVVAQGQVGSVMKVLRDLPDGVQYHVHFGDGRVLQVPERSLCMADPQAEVGDEQ"
     gene            2587..3468
                     /gene="nifM"
                     /locus_tag="VIO83_06390"
     CDS             2587..3468
                     /gene="nifM"
                     /locus_tag="VIO83_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifM"
                     /protein_id="HEY8330935.1"
                     /translation="MSSERLMSDGDSRYYLLKVAHEQFGRAPGELAAEQHEQAARIVA
                     RQRQIEEAVLRSPEASGVVIPAAQVEDAWASIGSRYAEGELQQALAGHGLDDVGLRAM
                     LARELKVEAVLERVCAGLPEVSETDVGLYYFSHLEQFEVPATRVARHILITINPDFPE
                     NTRESARVRIEAIAKRLRSKPERFAEQALKHSECPTALQDGLLGAIKPGTLYPQLEAS
                     LFELAEGETSPVLESPLGFHLLRCEAVQHARRLALEEVLPRLRDSLQNRQRKARQRQW
                     LASLLQPSAPVENQAHG"
     gene            3461..4789
                     /gene="clpX"
                     /locus_tag="VIO83_06395"
     CDS             3461..4789
                     /gene="clpX"
                     /locus_tag="VIO83_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY8330936.1"
                     /translation="MADTEKTCCSFCGAEKSPSVPLIAGTEGRICEACVTLAHKVVSS
                     WGQRRKSQQLAPQLRTPAAYKQHLDESVIGQEAAKETLAVAVYNHYLRLLNCDREPIC
                     QLGEEVELEKSNILMAGPSGTGKTLLVRSLARILGVPFATADATTLTQAGYVGDDVDS
                     IITRLLEAANGDVQQAQWGIVYIDEVDKLAKRSGGGTAVRDISGEGVQQALLKMVEGT
                     EVRIAKSGRRNEHSEEQVVDTRNILFIAGGAFPGLEALVCSRIRPKTGGIGFHAQPPR
                     QEQPSINELLAALLPDDLHEFGLIPEFIGRFPIITFLQELDHATLLRILTEPRNALVK
                     QYKQLFAYQGVKLEVSEAALNHIADQALTRKTGARGLRAVLEAALQQTMFSMPSKPLL
                     RGCTLDLVEGDAEWHLDVLETLAEEGDVEQADGRLLPEASPQQALMSVDL"
     gene            5014..5556
                     /locus_tag="VIO83_06400"
     CDS             5014..5556
                     /locus_tag="VIO83_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039800902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEY8330937.1"
                     /translation="MARIGLFFGTNTGKTRKVAKMIKKRFDDDTMADALNVNRATAED
                     VAQYQYLIIGTPTLGDGQLPGLSADCENESWEEFLPKLQGVDFTGKTVAIFGLGDQVG
                     YAAEFLDAMGLLNDFLVERGAKVVGAWPTEGYEFEHSEAVVDGKFVGLALDLDNQSGL
                     TEERLSTWLQLIAPDFDLSL"
BASE COUNT         1051 a         1918 c         2015 g          994 t
ORIGIN      
        1 atcgacaccc gcggcatccc ggcgatctcg gcgctggtcg agcaggcctc cggacgtcag
       61 gtggcctggc agaagagcgt ggtcggcgcc ggggtgttca cccacgaggc gggcatccac
      121 gtcgatggcc tgctcaagca ccggcgcaac tacgaggggc tgaaccccga cgaactgggg
      181 cgcagccaca gcctggtgct gggcaagcat tccggcgccc acatggtgcg cagccgctac
      241 cgcgagctgg gcatcgagct ggacgactgg cagtgccagg cgctgctcgg gcgtatccgc
      301 gtcttctcca ccggcagcaa gcgcagcccg caggccgccg aactggaaga gttctatcgc
      361 cagttgggcg aacaaggcgc gccggtcctg gctgcaggag gtcgggcatg agcctgttcg
      421 gcgaatggcg cgacgatatc cgctgcgtgt tccagcgaga tccggcggcg cgcacccgct
      481 tcgaggtgct caccacctac ccgggggtgc acgcgatcct gctctatcgc ctcgcgcatc
      541 gcctgtggcg ggccgagtgg cgctatccgg cgcggctgct gtcgttcctc gcacggctgg
      601 tcagcaatgt cgacatccat cccggcgcgg tcatcggccc gcgcttcttc atcgatcacg
      661 gcgccggcgt ggtgatcggc gagaccgccg agatcggcgc ggacgtgacc ctctatcacg
      721 gcgtcaccct cggcggcacc agctggcgca agggcaagcg ccatccgacc ctcggcgacg
      781 gcgtgctggt cggcgccggg gcgaagatcc tcgggccaat cagcatcggc gccggcgccc
      841 gggtcggcgc caactcggtg gtggtgcagg acgtgccgga gggctgcacg gtggtcggca
      901 tccccggcaa ggtggtcagg ctgcgcgagg ccggccagcc caacccctac ggcatcgacc
      961 tcgaccatca cctgattccc gacccggtgg gcaaggccat cgcctgcctg ctcgaacgcc
     1021 tggacagcct ggagaagcag gtcgaggcgg gcggtcgggt ggccgccggc agccagcagc
     1081 agtactacca gggctgcagt gtggacaaca gcatctgcga aagcgactgc gccggtggtg
     1141 ccgtcgccct cggccaggca ctgcccctgc ggcttgccgc cagtccggct ggcgactgag
     1201 cgggcaaaca acggaggcgc gcgcatggac ctgcaggact tcaatggcga tggcctgtac
     1261 ttcgacgaag cgcagtcggc ggcggtcgac agcctgctcg ccgccgccgc ggaacagtac
     1321 ggcgagggca cggccgagca gcccttgctg gcggcccagg cgctggcccc ggacgatctc
     1381 agcgtgctgg tcggcctgta ccgcttctat tactaccagc accgctatca ggacgccctg
     1441 cagatcgccg agcgagtgct ggcggtggtc gggccgcgcc tcaagctgcc agcgcgctgg
     1501 caggcgctcg acgccagcca cctcgccatc gcggcggggc gcggcatcgg cctgttgcgt
     1561 ttccatctgc tggcgctcaa gggcgccggc tacctcagcc tgcgcctggg cctcttcgag
     1621 cagggcaagg ccatgctcgc caaggtcgtc gagctggatg cggacaaccg cctgggcgcc
     1681 aggctgctgc tggatgtgct ggcagcgaac aacgccgaga tcgtcacttt ccccactgcg
     1741 gccaccgcgg agacacgacc atgaccgtac aaggcttttc cccggattcc gacctgacgc
     1801 tggacgaggc catggaggag ctggtgtccg ccgaagactt cctcgacttc ttcgcggtgc
     1861 ccttcgcgca gggtgtcgtg cacatcaacc ggctgcacat catgcagcgc taccacgact
     1921 atctgaccaa ggccggcagt ctggacgcgc atgacgatgt agcgcgcgcg gccatctacc
     1981 gccagctgct ggcgcgtgcc taccaggatt tcgtcgactc cgacgcgcag accgagaagg
     2041 tcttcaaggt cttccgcatg catgagccgc agaccacctt cgtctccatc gaccagctgc
     2101 tgagctgacc aggggagatc agggccatga gtctgccgca attcgagtac ggcgatgagg
     2161 tgcgcgtggt acgcaatgtg cgcaacgacg gcacctatcc gggcatggat accggcaccc
     2221 tgctgatccg ccggggttcg gtgggctgtg tgtacgacgt cggtacctac ctgcaggatc
     2281 agctgatcta ccgggtgcac ttcctcgagt cgggacgcac cgtcggctgc cgcgaggagg
     2341 agctgatccc cgcctcggcg ccgtggacgc caagcctctt cgagtttcgc gacaacgtca
     2401 cggccatgcg cagcttcgcc gtgcgtggcg aagtggtggt ggcgcagggc caggtgggca
     2461 gtgtgatgaa ggtgctgcgc gacctgcccg atggcgtgca gtaccacgtg cacttcggcg
     2521 atggccgggt gctgcaggtc cccgagcgga gcctgtgcat ggccgatccg caagcggagg
     2581 tgggtgatga gcagtgaacg actgatgagc gacggcgaca gccgctatta cctgctgaag
     2641 gtggcccacg agcagttcgg ccgggctccc ggcgaactgg ccgcggagca gcatgagcag
     2701 gctgcacgca tcgtcgcccg ccagcggcag atcgaggagg cggtgctgcg cagcccggaa
     2761 gcgtccgggg tggtgattcc cgccgcacag gttgaagacg cctgggcgag cattggcagc
     2821 cgctatgccg aaggcgagct gcagcaggcg ctcgccggcc acggtctgga cgacgtcggc
     2881 ctgcgtgcca tgctggcccg ggagctgaag gtcgaggcgg tgctcgagcg cgtctgcgcc
     2941 ggtctgccgg aagtcagcga gaccgacgtc ggcctgtact acttcagtca tctcgagcag
     3001 ttcgaggttc cggccacgcg cgtggcgcgg cacatcctga tcaccatcaa tccggacttt
     3061 ccggaaaaca cccgggagtc cgcacgggta cgcatcgagg ccatcgccaa gcgtctgcgc
     3121 agcaagccgg agcgcttcgc cgagcaggcg ctgaagcatt cggaatgtcc gaccgcgctg
     3181 caggacggcc tgcttggcgc catcaagccc ggcaccctgt atccgcagct ggaggccagc
     3241 ctgttcgaac tggcggaggg ggagaccagc ccggtgctgg agtcgccgct gggtttccac
     3301 ctgctgcgct gcgaggccgt ccagcacgcc cggcgcctgg ctctcgagga agtcctgccg
     3361 cgcctgcgcg acagcttgca gaaccggcag cgcaaggctc gccagcggca atggctggcg
     3421 agcctgctgc aaccatccgc ccctgtggag aaccaggccc atggctgaca ccgagaaaac
     3481 ctgctgctcg ttctgcggcg cggaaaagag tccgagtgtg ccgctgatcg ccggcaccga
     3541 ggggcgtatc tgcgaggcct gcgtcacact ggcccacaag gtggtgagca gctgggggca
     3601 gcggcgcaag agccagcagc tggcgccgca gctgcgtacc ccggcggcct acaagcagca
     3661 tctggacgag tcggtgatcg gccaggaggc ggccaaggaa accttggcgg tggcggtcta
     3721 caaccattac ctgcgcctgc tcaactgcga ccgcgagccg atctgccagc tcggtgagga
     3781 ggtcgagctg gaaaaatcca acatcctcat ggccggtcca tcgggcaccg gcaagaccct
     3841 gctggtgcgc agcctggcgc gcatcctcgg cgtgccgttc gccacggccg acgccaccac
     3901 cctgacccag gccggctatg tcggcgacga tgtcgacagc atcatcactc gtctgctgga
     3961 ggccgccaat ggcgacgtgc agcaggcgca gtggggcatc gtctacatcg acgaggtgga
     4021 caagctggcc aagcgcagtg gcggcggcac ggcggtgcgc gacatttccg gcgaaggggt
     4081 acagcaggcc ctgctgaaga tggtcgaggg caccgaagta cgcatcgcca agtccgggcg
     4141 gcgcaatgag cacagcgaag agcaggtggt ggatacgcgc aacatcctgt tcatcgccgg
     4201 cggcgcgttt cccggcctgg aggccttggt gtgcagccgt atccggccga agaccggcgg
     4261 catcggcttc cacgcccagc cgccgcgtca ggagcagccg tcgatcaacg agctgctggc
     4321 cgccctgctg ccggacgatc tgcatgagtt cggtctgatc cccgagttca tcggccgctt
     4381 cccgatcatc accttcctgc aggagctgga ccacgccacc ctgctgcgca tcctcaccga
     4441 gccgcgcaat gcgctggtca agcaatacaa gcagctgttc gcctaccagg gcgtcaagct
     4501 ggaggtcagc gaggcagcac tgaaccacat cgccgatcag gctctgacgc gtaagaccgg
     4561 tgctcgcggc ttgcgtgccg tgctggaggc ggctctgcag cagaccatgt tcagcatgcc
     4621 atcgaagcca ttgctgcgtg ggtgcacgtt ggatctggtc gagggtgacg cggagtggca
     4681 cctcgatgtg ctggaaaccc tggcagagga gggcgatgtc gagcaggcgg atggccgcct
     4741 gctgcccgag gcaagcccgc agcaagcgct gatgtcggtc gacctgtgat tgtcgcaata
     4801 ccgacagggc gcgttgttgg cacgcttccc ggctgtcggt tccctgacaa agacgctttc
     4861 ttgaaatgga aaagctttac gtatcagtag gttgtgagtg cttttgggcg ttgctgaagt
     4921 ggcccgtttc ctgctgataa ccaatgcccg cctggtcaaa ccgaggcagg cagccccgaa
     4981 aacggatctc atgcacgcag tcagaggtga gctatggcca ggattggact cttcttcggc
     5041 accaatacgg gcaaaacccg caaggtggcc aaaatgatca aaaagcgctt cgacgacgac
     5101 accatggcgg acgcgctgaa cgtcaatcgc gccaccgctg aggacgtggc ccagtaccag
     5161 tacctcatca tcggtacacc aaccctcggc gatggccaac tgcccgggct gtcggccgat
     5221 tgcgagaacg aaagctggga agagttcctg ccgaagctgc agggtgtgga tttcaccggc
     5281 aagaccgtgg ctatcttcgg cctgggtgat caggttggct atgccgcgga gttcctcgac
     5341 gccatggggt tgctcaacga cttcctcgtc gagcgtggcg ccaaggtggt cggtgcctgg
     5401 cctaccgagg gctacgagtt cgaacactcc gaagcggtgg tcgacggcaa gttcgtcggc
     5461 ctggccctgg acctggataa ccagagcggc ctcaccgagg agcgcttgag tacctggttg
     5521 cagctgattg cccccgactt cgatctgagc ctgtaaggcg ggcagagccc ggtcatgcgc
     5581 cgcaacggcc catgaccgcc tgcgggctcg ggatctcttc cgcagcacgc cttggcctcg
     5641 gaaagtctcc agcgcctggg gtgacgccat atagcagagc agtactcgcc atttgccggg
     5701 tgctgaagtt gctccggtat tttcctggac aggtgttgac agcgttttta aaggctgtat
     5761 tattcgccac ctcgctgcgg cggcctccgg gttgaagcta gcgcaagtgg ttgaaacgaa
     5821 aagaaatttt cacgaagcgc ttgacaggtt gtaaggctgc tgtagaattc gcggcctcgg
     5881 ttgagacgaa acactggctc ggtcgaaacg ctctttaaca agttgaatca agcaattcgt
     5941 gtgggtgctt gtgagataag actgacagtc gcaagatt
//
LOCUS       DATCUN010000158         4720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_81307, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000158 DATCUN010000000
VERSION     DATCUN010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81307"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="VIO83_06405"
     CDS             complement(<1..141)
                     /locus_tag="VIO83_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020289628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEY8330938.1"
                     /translation="MNQASLSAFIWSVADLLRGDYKQSEYGRVILPFTVLRRLDCVLE
                     ATK"
     gene            620..1771
                     /locus_tag="VIO83_06410"
     CDS             620..1771
                     /locus_tag="VIO83_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330939.1"
                     /translation="MFHKHWRLLLGLFFLGFAVVALTRNFIVVTATDAFINANLIAVR
                     SPIPGQIARPLPPVGSRTGTSTLAQVRNTRADQTLTLEISQRLEQVDSALSGASARLE
                     KLKAAEREFAAWSRRYVGARGVYLSQRQSQAAANREASLRQLENARSQLKRLEGVQAY
                     ISRDMLEQARTSAQVAEQSLLAATAETEQVASERAALGQRLQIADSYSERTYSEQKLQ
                     ETRLQAAMLEADIMSLQQQRKALADNLQRSQGQLGRESLANVTVPDGAVWRRLPVGAH
                     VERGGDLGQVAPCDDAVVTATLDRNSFDKLYVGMPVRASFRLEDGSRHTVELALVTLT
                     GGSTENALGMAIPFGRAIVEDAFGAVLRARDPSALGCAIGRPVDLKFGA"
     gene            1782..3800
                     /locus_tag="VIO83_06415"
     CDS             1782..3800
                     /locus_tag="VIO83_06415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8330940.1"
                     /translation="MLNALIPSLLIISMLLLAPEVSRILRGHDLPVPRGRWRALIFVP
                     LLLLQLRYLHWRLTSTLPELSLSPEGLWPLLFLVFEMTTMLTLAMTYLTLTRRSDRTP
                     EVEANLGLLGQAEARVAVFIPTYNEPWEVLERTLLGAISQRFPGVRVCVLDDSRRGWL
                     RARCAEFGVEYCQRDNNLGAKAGNMNAGLATLRARGFDPEFIAVLDADFIAKPDFVSR
                     CLVLHLLHPDAGLVQTPQHFFNPDPVQMNLVLTGTAPDEQRFFFDELLPSKDAWGTAF
                     SCGTSALIRTRALDAIGGFPTESVTEDMLMSLRMREHGWQTLYLNEPLSTGLSPQGCS
                     EYIKQRHRWCLGFIQIVRNIYNPFSTRHRLGLRDRVQLLDTFLYWAVSFPARYVFLLA
                     PVVYWVTGWQVVVAAIEQILLHLLPWLLAHALVMAWISRGSLQPVLSDVTQLLTGRAA
                     IHATLTGLFSKGEHKFVVTPKGDLGHAVRIDWQTMSPILILAGLNLCGILIALFPSAW
                     HHDLPFEPRVVNLFWLIVNSLILALTALACIERPRRRVQERMQLDDPLAARVQAGPVT
                     WRAQLFDLSLSGCHVDIDAAGHTLDRLVGNEVVLELGNNERYRLELVRRSDATHFGGR
                     FVDLSMDQRMRLFERVFAENPAYLRHQTGSSEVLAKMLHNLWSRRGHR"
     gene            complement(3826..4134)
                     /locus_tag="VIO83_06420"
     CDS             complement(3826..4134)
                     /locus_tag="VIO83_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY8330941.1"
                     /translation="MGFDVRFTEQAQQDIERLYEWLLQRAQGDFSVAERALQAIGDAI
                     TVLELAPLSCRKADPGGAFLRELVIDFGASGYVLLFEIENSQTVTVLAVRHQREDDYY
                     "
     gene            complement(4140..4403)
                     /locus_tag="VIO83_06425"
     CDS             complement(4140..4403)
                     /locus_tag="VIO83_06425"
                     /inference="COORDINATES: protein motif:HMM:NF041551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HEY8330942.1"
                     /translation="MKSASLPSLRVDPALREAAEAVLREGETLSSFVEQSVRAQVQLR
                     RQQEEFMARGLASRECARQSGRHVQARDVLAALKSRLDKARQG"
BASE COUNT          790 a         1577 c         1549 g          804 t
ORIGIN      
        1 cttggtcgcc tccagcacgc agtccagccg ccgcagcacg gtgaagggca ggatgacgcg
       61 gccatattcg gattgcttgt agtcaccacg cagcaggtcg gccacggacc agatgaaggc
      121 ggaaagcgat gcttggttca aggtgatggt tccggggaag gcatgggggc tgggcggtgg
      181 ccgctcatgc accacgccgg atcaccaacc gggcctcagg tcccggccag ggccgcccca
      241 ggcgatgcgg tgccgatgcg gcggctcgcc accgccgccg cgcgctgcat gttggcaggc
      301 cggagcgccc ttcgccaagc gcagcctggt gtccgcgcct caggtcggcg gtgctggccg
      361 gaaaaagcgc ataaacggtg cgaagtgacg caggaggtca cactaaaacg actgaaaccg
      421 gcccgaaacc tggctccatg gcgatgtggc cggcgcacgg atcggaacct gccgccggcg
      481 cggacccggc gcttggcatg gctcttgcat ccggcactgg cagccgtgcc tttccgcagg
      541 ctgcctcccc cctttctcca ggccccttcg acggggacgc acccgcatgc ccgcaccttt
      601 gtccaacctc cttacgaccg tgttccacaa gcactggcga ctgctgctgg gcctcttctt
      661 tctgggcttc gcggtggtgg cgctgacgcg caatttcatc gtcgtcacag caaccgacgc
      721 cttcatcaac gcaaacctga tcgccgtgcg cagccccatt cccggtcaga tcgcacgacc
      781 gctgccgccg gtcggcagcc ggactggtac ctctaccctg gcccaggtgc gcaacacgcg
      841 cgccgaccag accctgactc tggagatctc ccagcgcctg gaacaggtgg actctgcact
      901 gtccggagcg agcgcgcgcc tggaaaaact gaaggcggcg gaaagggagt tcgccgcgtg
      961 gtccaggcgc tatgttggcg cgcgcggcgt ctacctcagc cagcgccaat cccaggcagc
     1021 ggccaaccga gaggccagcc tgaggcaact ggagaacgcc cgcagtcagc tgaaacggct
     1081 ggaaggcgtg caggcttata tatcccgcga catgctcgag caggcccgca cttcggcgca
     1141 ggtggccgag cagtcactgc tggcggcgac cgccgaaacc gagcaggtgg cctccgaacg
     1201 tgcagccctg ggccagcgcc tgcagatcgc cgacagctac tccgagcgca cctactccga
     1261 gcagaagctg caggagacgc ggctgcaggc cgcaatgctg gaagcggaca tcatgtcact
     1321 gcagcaacaa cgcaaggcgc tggccgacaa cctgcaacgc agccaaggcc agttggggcg
     1381 agaatcgctg gccaacgtca ccgtgcccga cggagcggtg tggcggcgcc ttccggtcgg
     1441 cgcgcatgtc gagcgcggcg gcgaccttgg ccaggtcgcg ccctgtgacg atgccgtagt
     1501 caccgccacc ttggaccgca actcgttcga caagctgtat gtgggcatgc cggtacgggc
     1561 aagcttcagg ctggaggacg gcagccggca cacggtcgaa ctggcactgg tcaccctgac
     1621 cggcggttcc accgagaatg cactgggcat ggccatcccg ttcggacgcg caatcgtgga
     1681 ggacgcattc ggtgcggtgc tcagagcacg cgacccgtct gcgctgggct gcgcgattgg
     1741 caggcccgtg gacctgaagt tcggggcatg agggcgatca ggtgctgaat gcgctgattc
     1801 ccagcctgct gatcatcagc atgctgctgc tggcgccgga ggtgagcagg atcttgcgcg
     1861 gtcacgatct gcccgtgcca cgcggccgct ggcgcgcgct gatcttcgtg ccactgctcc
     1921 tgctgcagtt gcgttacctg cactggcgcc tgaccagcac actgcccgag ctttcgctgt
     1981 cgccggaggg gctatggccg ctgctgttcc tggtgttcga aatgaccacg atgctgacgc
     2041 tggcgatgac ttacctgacc ctgaccaggc gcagcgaccg gacgccggag gttgaggcca
     2101 acctcggcct gctaggccag gccgaagccc gggtggccgt gttcattccc acctacaacg
     2161 agccttggga ggtgctggaa cgtacgctgc tgggcgccat ctcacagcgt tttcccggcg
     2221 tgcgcgtgtg cgtgctcgac gacagccgcc gcggctggct gcgcgcgcgc tgtgccgagt
     2281 tcggcgtgga gtactgccag cgagataaca accttggggc aaaggccggc aacatgaatg
     2341 ccggcctggc aaccttgcgt gcgcgcggct tcgacccgga gttcatcgca gtgcttgatg
     2401 ccgacttcat cgccaagccg gattttgtca gccgttgcct ggtgttgcac ctactgcatc
     2461 cggacgcggg cctcgtacag acaccgcagc acttcttcaa ccccgatccg gtgcagatga
     2521 acctggtgct gaccggcacc gcaccggacg aacaacgctt cttctttgac gaactgctgc
     2581 cgtccaagga cgcctggggg acggccttca gctgtggcac ctcggcactg atccggacga
     2641 gggcgctgga cgccatcggc ggttttccga ccgagagcgt caccgaggac atgctgatga
     2701 gcctgcgcat gcgcgagcat ggctggcaga cgctgtacct gaatgagccc ctgagcaccg
     2761 ggctgtcgcc gcagggctgc agcgagtaca tcaagcagcg gcaccgctgg tgcctgggct
     2821 tcatccagat cgtacgcaac atctacaacc cgttctccac ccgccaccgg ctgggcctga
     2881 gggaccgcgt gcagctgctg gacaccttcc tgtactgggc cgtgagcttc cccgcgcgct
     2941 acgtcttcct gctggcgccg gtggtgtact gggtaacggg ttggcaggtc gtcgtggcag
     3001 ccatcgagca gatcctgctg catctattgc catggctgct tgcgcatgcg ctggtgatgg
     3061 cctggatctc gcgtggatcg ctgcagccgg tgctcagtga cgtgacccag ctgctgaccg
     3121 gtagagccgc gattcacgcg acgctgaccg ggctgttttc caagggcgag cacaaattcg
     3181 tggtgacgcc gaagggcgac ctggggcacg cggtgcggat cgactggcaa acgatgagcc
     3241 ctatcctgat cctggccggc ctcaacctgt gcggcatcct gatcgcactg ttcccgtcgg
     3301 cctggcacca cgacctgccg ttcgagccgc gtgtggtgaa cctgttctgg ctgatcgtca
     3361 acagcctgat cctggcgttg accgcactgg cctgcatcga acgcccacgc cgccgcgtac
     3421 aggaacgcat gcagctggat gatccgctcg cggccagggt gcaggccggt cccgtgacct
     3481 ggcgcgcgca gctgttcgat ctgtcgttgt ccggctgcca cgtcgacatc gatgccgcag
     3541 gccatacctt ggaccggctg gtcggcaacg aagtcgtgct ggaactcggc aacaacgagc
     3601 gctatcgcct ggagctggtg cgccgcagcg atgccacaca tttcggcggc cgcttcgtcg
     3661 acctgagcat ggaccagcgc atgcgcctgt tcgagcgcgt gttcgcagaa aacccggcct
     3721 atctgcgcca ccagactggc agcagcgaag tgcttgccaa gatgctgcac aacctgtgga
     3781 gccgccgcgg ccaccgctga agtgaccttg tgcggacacg tccgttcaat agtagtcgtc
     3841 ttcgcgctgg tgcctgaccg ccaggaccgt cacggtctgg ctgttttcga tttcgaacag
     3901 cagcacgtag ccgctggcgc cgaagtcgat gaccagttcg cgcaggaaag caccgcctgg
     3961 gtcagccttg cggcagctca ggggtgccag ttccagcacg gtgatggcgt cgccgatggc
     4021 ttgcagggcg cgctcggcca cgctgaaatc gccttgcgcg cgttgcagca accactcgta
     4081 gaggcgctcg atgtcctgtt gcgcctgctc ggtgaaacgg acatcgaagc ccacccgatt
     4141 cagccctggc gcgccttgtc cagtcgagac ttcagagcgg ccaggacgtc cctggcctgg
     4201 acatggcggc ctgactgccg ggcgcattcg cgcgatgcca gcccacgggc catgaactcc
     4261 tcctgctgcc ggcgcagctg gacctgggcg cgtaccgatt gctcgacgaa gctggagagg
     4321 gtttcgccct cgcgcaggac ggcttcggcc gcctcgcgca gggcaggatc aacccgcagg
     4381 gaaggaaggg aggctgattt catgccggtc accacgtgcg ttgcaattgc gatgcatggt
     4441 gtcaggatgc ggacaccgct tcaagcgcac gcagccccgc atgcgcggcc gcgccggcct
     4501 tgacccttct gctgcctcaa cccccactgg agcttcgaca ggcgtcaaac gcgcagccgg
     4561 gcgcgggtct ggacgccgct catcgcgaag gttcgccgcc ggcatgcggc tgggccgagg
     4621 ccagttccgg atgccagagc tcgtgggtgt ccacgcgcac cagcaggatc gcctgcaggg
     4681 cctgcacccg cgccagggcc tcggccagct gggcctggcg
//
LOCUS       DATCUN010000159        36224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_44522, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000159 DATCUN010000000
VERSION     DATCUN010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 36224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36224
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44522"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4233)
                     /locus_tag="VIO83_06430"
     CDS             complement(<1..4233)
                     /locus_tag="VIO83_06430"
                     /inference="COORDINATES: protein motif:HMM:NF033682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retention module-containing protein"
                     /protein_id="HEY8330943.1"
                     /translation="MAKLIGFVRAVVGEVFAVAADSSKRQLGEGDRVFAGEQLLTGAG
                     SAVAIRLVNGGELTVGRDSSLMLDTQLLAGENARAPIQPATPEHVPSAQELAEVEEVQ
                     QAIASGVDPTLSLPATAAGPGTGGPAGSGGGGISFVMLGETGDRVDPNIGYPTGPLQF
                     APLFPEIFPQGADEELDPIPSIRVEYFADATGQVLTERGVVDEAALPGGSRAESPAES
                     TFGRVVISSLDGIQAIQVQDKDGNWIDVLDGGVVQGQYGTLQVAPNGTWVFTLNGNTL
                     DHKDPNATTTDEVVQSFPVRVIDADGDISPPASLVINIGDDGPQAINDSVLTPEDTPV
                     TVNVLGNDIQGVDSGATLVSAVLTGGSGTVSFLPDGSVTFTPAPGFEGLASISYVMQD
                     ADGDTSQAVLTVGVGVDSVPDLAITYGPGAGVVDEAALNAGGSGDGLPGSNPSSTAEQ
                     TSGSIVVDTKGDSLGSVTISSVVIGGVDVTNGGTVSGQYGVLQVSKLTEGEYSWTYTL
                     TDNSLNHEGANQTGPADQLFDVFSVVATDNEGDPVGPQALTIAINDDGPTAVNDSVLT
                     PEDTPVTVNVLVNDVQGSDTATLIGASLTSGNGSVSFLPDGAVTFSPAPGFEGVATIA
                     YTILDADGDTSQAVLTVGVGVDSVPDLAITYGPGAGVVDEEALNAGGSGDGLPGSNPS
                     STAEQTSGSIVVDTKGDSLGSVTISSVIIGGVDVTNGGAVDGQYGVLQVTKIGEGEYS
                     WTYTLTDNSLDHAGVNLTGTDDQLFDVFSVVATDNEGDPAGPQALTIAINDDGPTAVN
                     DDSSLSEDATSVSGNVLVNDIAGADAGAVVTTPGTSIGSYGQLVLGAGGAYTYTLNTA
                     SPVVQGLDTGASLTETFAYTMRDADGDESSATVTITITGSNDVPSLTVDPGNGGANDV
                     VDEAGLSSGSDAASPSEFASGTFTLSDADGLDDLQSVTINGITVAIGSLAGSSFAGAS
                     GTLTITAYDAATGVATYTYQLTSPTTDLAGIETDTFTLSVSDGTTPSAPATITIDILD
                     DLPNAEDDVPASLVEDGLSLVEGNVLSNDEHANGQIGADGRFFVAWENSTANILSTYG
                     TVVLGDDGSYRFVLDNTRPATQALNAGEVVSETFTYTIRDADGDTDTAILTLSVTGTA
                     DGVVLGGLDGTGSEIVVDDANLADGSAANAALLTQTGSFTFSAPDGVQSLTVGGSPLI
                     SGGVLAGTLPTLTSGLGNTLTITGVDYDPATGGGTVFYSYTLLDNEMHTQPANDTALS
                     ESFAVELVGSGGSTTSQSLDVTILDDVPNAVSGKPASLVEDGLSLIEGNVLLNDVHTN
                     GQIGADGRFFVAWEDSTANILSTYGTVVLGDDGSFSFVLDNTLPATQALNAGEVVSET
                     FTYTIRDADGDTDTATLILRVAGADDGAVLSGL"
     gene            4704..6056
                     /locus_tag="VIO83_06435"
     CDS             4704..6056
                     /locus_tag="VIO83_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY8330944.1"
                     /translation="MRHTSMWVGLLLAAGFNQASAMSLTEAIQSTIDSHPEIHAANNS
                     RLSADEELKIAQGGYLPTVDMLASYGREQTDSPSTRIIGDHNTETLNSRTAELRLRQM
                     VFDGFYTPNEVARNEAVVNSRAYRALGATESLALRTIEVYLDVLKRREMVALAENNLQ
                     AHTRIHDQISMRAERGVGSTADRDQSQARLALAENNLYTEQVNLADAEANFFSAVGRM
                     PDELETPASIRGQMPESVASARDRVMDNSPLLKSAQSDVQAAEKQYESAKSKFYPRFD
                     VELSGNADDNIQGDEGHYNRWYAGVVMNYNLFNGMRDKALLQSGAHKINESMDIRNNA
                     LRILNENLMLSWNAMENARLQTPKARDYASYTSRVREAYQQQFSLGQRTLLDLLDSEN
                     ELFSANRRYTDVRYTEEFSMYRVVASMGDLLRTHNVVPPSEALARTEVKSEARLPSMK
                     "
     gene            6124..8283
                     /locus_tag="VIO83_06440"
     CDS             6124..8283
                     /locus_tag="VIO83_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016985929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEY8330945.1"
                     /translation="MTTMEQVGKADDPRHMHDDPLLDGLLILSRLHGRTVSRASLSAG
                     LPLPGQRLSPDLLTRAAARAGLQARLLRRPLEAISALNLPVLLLFKDGHCAVLRRWND
                     KGQAQILPCEADGGEQWVEREQLAAEYAGQALFARPRHELEELRTPLVPRVKSWFRDT
                     LKLSRGLYTDAILASLLINLLGLMVPLFVMQTYDRVVPNQALSTLWVLTIGLLIGTLF
                     ELLLRVLRFRLLDVAGKKTDVVLSATLFERITGMMMKSRPASIGGFAQSIHDFQGLRE
                     FLTAVTLTSLIDLPFALLMTVVIGLLGGWLVVIPLLAFPITALFAYVIQHRLRDTVQR
                     SLALGAERQALLIETLGGLETLKSCGAESERQHQWESTHGALSRLESHARLLSAVALN
                     GTLFLQQLAGVAMIVAGVYSIIAGNLSVGALVACYMLNGRIMAPLGQVASLITRHQQA
                     QLTMKSTDMLMNLPQERDSQQRPLEHTRLHGALELRQVAFSYAEQSSPALQGINLRIA
                     PGEKIAIIGRSGSGKSTLARLLMGFYSPSEGQILFDGIDQRQIDVADLRHQIGYVAHD
                     LPLLAGTLRSNMTLGARYISDERLLEVAELTGVSELARQHPQGFERPVGERGQLLSGG
                     QRQAVLLARALLLNPPLLLLDEPTSSMDNSSEEVLRQRLHDWSRSKTLLLITHRTSML
                     SLVDRLIVLDGGKIVADGPKETVMEALRKGRIGPASV"
     gene            8294..9649
                     /locus_tag="VIO83_06445"
     CDS             8294..9649
                     /locus_tag="VIO83_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEY8330946.1"
                     /translation="MSTLQSLGGYLNPLRQSRDTEFMPEVQGALVEDSTGLTRLTVWL
                     TALFLLTALVWAHFAVLDEVTTGEGKAIPSSKVQTIQNLEGGIVAEIFVREGQVVDKG
                     DPLLRLDDTRFVSNMSESEADRLALLARVERLSAEGEGRPLQLPEEIAKVAPGLAADE
                     EALYRSRQQGLSSEVSILQEQLRQKTQELAEFRAKAQQYRSSLGLIQSELNMSEPLVA
                     SGAISQVEVLRLRRSAVETRGSLNATNLAIPRAESGVKEVERKIEEARLTFRTNALKE
                     LNEVRTELSKITASSRAIEDRVSRTGVVSPVRGIVKQLKVNTIGGVVQPGSDLLEIVP
                     LEDSLLIEARIRPQDVAFLHPGQQAMVKFTAYDYTIYGGLKAKLEMIGADTITDEDGK
                     SFYLIQLRTDKSHLGSVDKPLLIIPGMVANVDIITGQKSVLDYLLKPVLKARSEALRE
                     R"
     gene            complement(9653..10276)
                     /locus_tag="VIO83_06450"
     CDS             complement(9653..10276)
                     /locus_tag="VIO83_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HEY8330947.1"
                     /translation="MSRVQCPRCARPLTHCLCPLIPHLASHTRVLILQHPDETRHALN
                     TARLAALGLANAELRIGEHFPELESELAMDGVPAWLLFPGEGTQTVEQLAASAAGEPV
                     ERLLIVPDGTWRKARRLLYCNPWLAGLPRLALEDGLVSRYRLRKAPAAGALSTIEAIV
                     HCLNGLEGAGRFDALLRPFEALIEMQIAAMGEEVYRRNHLRGDAPAE"
     gene            complement(10323..11240)
                     /locus_tag="VIO83_06455"
     CDS             complement(10323..11240)
                     /locus_tag="VIO83_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8330948.1"
                     /translation="MSATRQGVDAFALQLMVLLCGIWGLQQVGIKLAAPDMAPLLQVG
                     LRSGIAAVLVALFMLWRRQPLALGDGTLRAGLLVGTLFALEFFFVAEGLRHSSAAHIV
                     VLLYTAPIFAALGLHWRVPSERLRRLQWLGIVLAFAGIILAFAGGLLGQQLSAGMLLG
                     DLYGLLAGMSWGALTVSVRCSRLSEAPAAKTLLFQLVCGCLLLLPLAWLGGQTEVVRL
                     TPLVWGNLLFQGVVVAFASYLVWFWLLRRYLATGLGVFSFMTPLFGVCFGVLLLGERI
                     DALFIGGGLLVLFGISLVSADAWWRRLLS"
     gene            complement(11409..12590)
                     /locus_tag="VIO83_06460"
     CDS             complement(11409..12590)
                     /locus_tag="VIO83_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8330949.1"
                     /translation="MLLNLFNEMRAAKVPVSLRELLDLLAALERQVVFADMDAFYFLA
                     RTVLVKDERHFDKFDRAFAAYFNGLQSLDAALEALIPEDWLRKEFERSLSAEDKAQLN
                     SLGGLDQLLEAFKQRLAEQKERHAGGNKWIGTGGTSPFGSGGYHPEGIRIGDAGQRQG
                     RAAKVWEQREYRNLDDQMELGSRNLQLALRRLRKFAREGAAEEFDLDGTIEHTARDAG
                     LLNIRMRPERRNAVKLLLLLDIGGSMDSHVKVCEELFSACRTEFKHLEYFYFHNFVYD
                     TVWKDNQRRHGERLATLDLLHKYGPDYKVVFVGDAAMGPYEISHAGGSVEHWNDEPGY
                     VWMQRFMDTYKKLIWINPYPQDAWKYTASTGMVRELVHERMYPLTPAGLEEGMRFLAA
                     N"
     gene            complement(12676..13521)
                     /locus_tag="VIO83_06465"
     CDS             complement(12676..13521)
                     /locus_tag="VIO83_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8330950.1"
                     /translation="MKFEGTASYVATDDLKLAVNAAITLQRPLLVKGEPGTGKTLLAE
                     QLAEALGTRLITWHIKSTTKAHQGLYEYDAVSRLRDSQLGVDKVHDVRNYIRKGKLWE
                     AFEAGERVVLLIDEIDKADIEFPNDLLQELDRMEFFVYETGETIKAAQRPIILITSNN
                     EKELPDAFLRRCFFHYIAFPDCATLQRIVDVHFPGIRQELVQEALEIFFDIRKVPGLK
                     KKPSTSELVDWLKLLMADQIGEALLRERDPGKAIPPLAGALVKNEQDVQLLERLAFMS
                     RRAGR"
     gene            13685..14659
                     /gene="cysK"
                     /locus_tag="VIO83_06470"
     CDS             13685..14659
                     /gene="cysK"
                     /locus_tag="VIO83_06470"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY8330951.1"
                     /translation="MSRIYADNAQAIGNTPLIRINRLGPQGVTILAKNEGRNPAYSVK
                     CRIGASMIWDAEQSGRLKPGMTIVEPTSGNTGIGLAFVAAAKGYKLVLTMPASMSLER
                     RKVLKGLGAELVLTEPAKGMKGAIDKASELAAAHPGEYLLLQQFENPANPAIHEQTTG
                     PEIWNDTDGAIDVLVAGVGTGGTITGISRYIKNTMGKPLLSVAVEPAGSPVISQTLAG
                     AEVKPSPHKIQGIGAGFVPKNLDLSIVDRVETVSDDEAKEMALRLMREEGILCGISCG
                     AAMAAAVRIAKEPQMQGKTIVVILPDSGERYLSTMLFDGLFGEQELQQ"
     gene            14945..15151
                     /locus_tag="VIO83_06475"
     CDS             14945..15151
                     /locus_tag="VIO83_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY8330952.1"
                     /translation="MATGTVKWFNDTKGFGFIKPDDGGDDLFAHFSEIRAQGFKTLAE
                     NQKVSFEVTTGPKGKQASNIQIIG"
     gene            complement(15258..16037)
                     /locus_tag="VIO83_06480"
     CDS             complement(15258..16037)
                     /locus_tag="VIO83_06480"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEY8330953.1"
                     /translation="MCELLGMSANVPTDIVFSFTGLMQRGGRTGPHRDGWGIAFYEGR
                     GLRLFQDPQASSESEVARLVQRYPIKSEVVIGHIRQANVGKVGLANTHPFVRELWGRN
                     WCFAHNGQLAGFAPQPGFYRPVGDTDSEAAFCDLLNRVRLDFPEPVEIAQLLPTLVQA
                     CADYRRLGVFNALLSDGDWLFSFCSTKLAHITRRAPFGPAQLKDADLRVDFQAETTPD
                     DVVTVVATEPLTANEAWALYLPGQWMLWRRGEVAACGQVAG"
     gene            complement(16064..16498)
                     /locus_tag="VIO83_06485"
     CDS             complement(16064..16498)
                     /locus_tag="VIO83_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8330954.1"
                     /translation="MTEQDYLLAWAVYAVAALGCLLVGFRITRWMWRGLREPLRVLMA
                     VLLLSPTLVDPVREFYAPAIAITALDLLMKTGGNLWRAISDLAMFGMIGFALYLLFVV
                     IRWPLLRRSRAKRQAAEAAREPTMREVLEQRNLDDGRVEPRL"
     gene            16584..18617
                     /locus_tag="VIO83_06490"
     CDS             16584..18617
                     /locus_tag="VIO83_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY8330955.1"
                     /translation="MSLTPPLARRQDGADPYAWLEQRDSAEVLDYLHAENAWLEQQLA
                     DQGELREQLFEEIRARIRETDLSLPTPWGPWLYYQRTTAGEEYPRHYRCPRPADGSLG
                     IDEAGEQLLLDPNLLAGDGYLDLGAFSISPDHRRLAYSLDTEGDEIYQLYVKELGTGE
                     VIALPFADCDGSMTWANDSRTLFFGELDDTHRPHKLYRHRLGEKDAELVFEEQDGRFF
                     LGCHRSSSERQLILLLASKTTSEAWVLDAEQPLGEFRCLAPRAEDHEYYPDHGQVDGQ
                     WCWLIRSNQAGINFALYRAEESAPTREHWQVLVPHREALMLEDFSLNAGAITLDLRDN
                     GLPVIEVLPRGAASYRVQLPDAAYSLHVYDDLEFDSPAIRLRYEALNRPAQIRQLELA
                     SGTQTVLKQTPVEGPFDADAYESRRVWATAADGTQVPISLVGRRDSFGRPAPLYLYGY
                     GAYGESLDPWFSHARLSLLERGFLFAIAHVRGGGELGEAWYRAGKLEHKQNTFGDFIA
                     CAEKLIADGYTTPAQLAISGGSAGGLLIGAVLNQRPELFAAAIAEVPFVDILNTMQNP
                     ELPLTVTEYDEWGDPSEPAVYERIRAYAPYENVSAQAYPAILAVAGYNDSRVQYWEAA
                     KWVAKLRASKTDANLLLLKTELGAGHVGMSGRYQALKDAALEYAFLLKVFGLA"
     gene            complement(18681..19904)
                     /locus_tag="VIO83_06495"
     CDS             complement(18681..19904)
                     /locus_tag="VIO83_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330956.1"
                     /translation="MFQRFKTPWAVLLLGLATPLAAAQRVDVDYRVRFLPDSDQAEVT
                     LTLERGQALRYLDFSLGDKGYYSDFQAQGEWQQSSPEHGVWRPQEGKAQLTYRVKISH
                     PRANGRFDARMTPDWALLRGDDLVPAAKIDVQDDVELVSRLQFELPEGWQSVETGWPR
                     VGKNRFRIDNPERLFDRPTGWILAGKLGTRRATLGKTDVAVAAPVGEGVRRMDILTLL
                     TFVWPHAQAVFPREPAKLLIVGAGDPMWRGGLSAPNSLYMHRDRPLVSENGTSSLVHE
                     LVHVFSRISDTDRSDWITEGIAEYYAIELMRRAGGLSEDRYQKVREQLTRWSRKVTSL
                     RGEQASGPVTARAVLLLQDLDAEIRQASGNRHSLDDVTRGLMRLDKASTRDFVQVSEN
                     LLGGPSKVLDTRLLR"
     gene            complement(19955..20404)
                     /locus_tag="VIO83_06500"
     CDS             complement(19955..20404)
                     /locus_tag="VIO83_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HEY8330957.1"
                     /translation="MAAKTEPFWKRKTLVQLDRDEWESLCDGCGLCCLQKLEDEEDGS
                     VYYTRIACKLLDRDTCRCSNYAERKQHVPDCIQLTPADAAEFRWLPPTCAYRLVAEGK
                     DLLPWHHLVCGDPQAVHDVRISQAGRMLAESSVAEEDWEEHLIFRAG"
     gene            complement(20517..20948)
                     /locus_tag="VIO83_06505"
     CDS             complement(20517..20948)
                     /locus_tag="VIO83_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8330958.1"
                     /translation="MSAGKIGRLLLGGLLVSLAACVQTPSSPGAGGSEVQQHCQLMQQ
                     QCLAIHGAKTPEERQKLMLEHRQAMMAGGMGMSGGCPAAGAHGGMGMGMGPGMMDGGM
                     GHGMGSGAMGAAPTAEQLDRQIEHVEMMLKQLKAQRAGMGK"
     gene            complement(21119..21409)
                     /locus_tag="VIO83_06510"
     CDS             complement(21119..21409)
                     /locus_tag="VIO83_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HEY8330959.1"
                     /translation="MKRICSIYKSPRREMYLYVDKREALKRVPEGLLGAFGAPQHVFD
                     MLLTPERKLAREDAAQVLENIEKQGYHLQMPPPEDDYIEHLPDELLRRNDPV"
     gene            complement(21418..22536)
                     /gene="rnd"
                     /locus_tag="VIO83_06515"
     CDS             complement(21418..22536)
                     /gene="rnd"
                     /locus_tag="VIO83_06515"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEY8330960.1"
                     /translation="MPAIDIRWILDDASLAACCSVWRTRSFVALDTEFMRVDTFYPIA
                     ALVQVSDGEAVWLIDPLQIRDWAPFAALLEDPAVVKVLHSCSEDLEVFKRLTGSLPQP
                     LFDTQIAAGYLNIGFSMGYSRLVKELLGLDLPKDETRSDWLQRPLSEMQVRYAAEDVE
                     HLCEVYRELEARLSADKRAWLLDDGAELVAAQREVSDPQEAWREVKLAWRLNGQQLAV
                     LRALCTWREEQARRRDQPRNRILRERSLWPLARTQPRDVVALARIEDMHPRTVRQDGE
                     TLLRLIAEAAALPAEHWPAQLPEPLPLEASALLKKLRAVGQREGERLQIVPELMLRKK
                     VLEALLKTGYPAGPYTLPDSLRGWRRALMGQALLDTLA"
     gene            22681..23535
                     /locus_tag="VIO83_06520"
     CDS             22681..23535
                     /locus_tag="VIO83_06520"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY8330961.1"
                     /translation="MSLARLISPQDLARRLGQPGLVILDCRFALEDPAYGRRAHAAEH
                     LPGACFADLEQDLSAPVIAGKTGRHPLPDPQQLCQRLREWGASNDSEIVLYDDGPGAF
                     AARAWWLLLWLGKREGVYLLDGGLSAWQGAGLPLEQHQPQPEPGDFAGSPDDSLVLGG
                     DELLQRLSDPELILLDARGLPRFRGEVEPIDPVAGHIPGAQCAAFIDNLGNDGRFLPA
                     EVLRRRFAGLLGMRPPEQLVAYCGSGVTACHNLFALCLAGYPLARLYAGSWSEWITDP
                     ARPVARDS"
     gene            complement(24033..25037)
                     /gene="rpoS"
                     /locus_tag="VIO83_06525"
     CDS             complement(24033..25037)
                     /gene="rpoS"
                     /locus_tag="VIO83_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HEY8330962.1"
                     /translation="MATTKTTPEFDRDDEVLLMELGVAPEMLSLNVDDEVESVASTTS
                     SSSARQHKFVDYTRALDATQLYLNEIGFSPLLSPEEEVHFARMARRGEAAGRKRMIES
                     NLRLVVKIARRYVNRGLTLLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIE
                     RAIMNQTRTIRLPIHVVKELNVYLRAARELTQKLDHEPTADEIATLLEKPVSEVKRML
                     GLNERVTSVDVALGPDSDKTLLDTLTDDHPTDPCELLQDEDLGQSIDLWLSELTDKQR
                     EVVIRRFGLRGHESSTLEEVGREIGLTRERVRQIQVEALKRLREILESNGLSSESLFQ
                     "
     gene            complement(25142..26005)
                     /locus_tag="VIO83_06530"
     CDS             complement(25142..26005)
                     /locus_tag="VIO83_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8330963.1"
                     /translation="MNETIRASNKELGQVGHISNWMPRLLGGLCLSLSLGLLGGCASS
                     SGTSETIGSQHGAGSSRQPVTHGQYRVRRGDTLYSIAFRYGWDWKALAVRNNIPAPYT
                     IRVGQVIRFGGSGPAVPMAATTPPPAAVQARGGNPVATRPQTVAPVSAAAAGNAGEAQ
                     AARSAAGWTWPANGQLISLFSSNSSLNKGVDIDGELGQPVLAASGGSVVYAGSGLRGY
                     GELVIIKHNDTYVSAYGHNRKLLVREGQQVKAGDKIAEMGATGTDRVKLHFEIRRQGK
                     PVDPLQYLPSR"
     gene            complement(26064..26699)
                     /locus_tag="VIO83_06535"
     CDS             complement(26064..26699)
                     /locus_tag="VIO83_06535"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY8330964.1"
                     /translation="MTSQRTRERLIQRLCEEGLSNAQVLEVIRRTPRHLFVDEALAHR
                     AYEDTALPIGHNQTISQPFMVARMTELLLAGGPLDKVMEIGTGSGYQTAVLAQLVERV
                     FSVERIQSLQDRAKERLLELNLRNVVFRWGDGWEGWPALTPYNGIIVTAAAAQVPQAL
                     LDQLAPGGRLVIPVGTGEEQQLMLIVREGERFSRQVLDPVRFVPLLSGSVF"
     gene            complement(26732..27478)
                     /gene="surE"
                     /locus_tag="VIO83_06540"
     CDS             complement(26732..27478)
                     /gene="surE"
                     /locus_tag="VIO83_06540"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY8330965.1"
                     /translation="MRILIANDDGVYAPGIVALHNALADYAECVVIAPDADRSGASSS
                     LTLDRPLHPQRLDNGFISLNGTPTDCVHLGINGLLEHIPDMVVSGINLGANLGDDVLY
                     SGTVAAALEGRFLDKPAFAFSLLSRQPDNLPAAAHIARRLVAMHDQLELPPRTVINVN
                     IPNLPLERIRGVQLTRLGHRGRAPAPVKSVNPRGKEGYWISVAGDAEDGGPGTDFHAV
                     MQGYVSITPLQLDRTFHEAFRGLSILENLL"
     gene            complement(27466..28536)
                     /gene="truD"
                     /locus_tag="VIO83_06545"
     CDS             complement(27466..28536)
                     /gene="truD"
                     /locus_tag="VIO83_06545"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEY8330966.1"
                     /translation="MSSVLGELDLLGLTAWGEPCGQAVLKHTAEDFQVNEVLDIPLSG
                     AGEHLWLWVEKRGLNTEDAARRIARAAGVPLKAISYAGLKDRQALTRQWFSLHLPGKA
                     DPDLAAAEGDDLRILERARHSRKLQRGAHAANGFIIRLTALQAEREALEQRLQQIAGA
                     GIPNYFGLQRFGFDGGNLHEARHWAERKTLPEQRNRRSRVLSAARSYLFNRVLAARVA
                     DGSWNRALPGDLLAFTDSRSFFPAGEAECSDPRLAALDLHPTGPLFGAGELETGGAVR
                     VLEQQVQVDDAVLVNWLADAGLEQARRVLRLPIGGLTWHYPATDILQLEFVLPAGCFA
                     TVLVRELVRLLPTGPVDTPCEY"
     gene            complement(28533..29006)
                     /gene="ispF"
                     /locus_tag="VIO83_06550"
     CDS             complement(28533..29006)
                     /gene="ispF"
                     /locus_tag="VIO83_06550"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY8330967.1"
                     /translation="MRIGHGYDVHRFGEGEFITLGGVRIPHRFGLVAHSDGDVLLHAL
                     SDALLGAAALGDIGRHFPDTDPQFKGADSRVLLRHVFALVQAKGWQVGNVDATIVAQA
                     PKMAPHIEAMRATIAADLGIVLDQVNVKATTTEKLGFTGREEGIVVHAVALLLAR"
     gene            complement(29074..29919)
                     /gene="fghA"
                     /locus_tag="VIO83_06555"
     CDS             complement(29074..29919)
                     /gene="fghA"
                     /locus_tag="VIO83_06555"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEY8330968.1"
                     /translation="MTLEIISSNKSFGGWHNRYRHRSATLNCEMVFAVYLPPQAEPGA
                     KLPVLYWLSGLTCTDENFMQKAGAQRLAAELGLVLVAPDTSPRGAEVPGDPDGAWDFG
                     HGAGFYLNATEQPWAQHYRMHDYVVHELPALVEASFPVSDRRGISGHSMGGHGALVCA
                     LRNPGRYRSLSAFAPIGNPSSCPWGEKAFSRYLGSDRDSWRQWDACALLATASKRLPI
                     LVDQGEADSFLAIQLKPEVLRAAAAAVGHPLTLRLQPGYDHSYYFIASFIDDHLRHHA
                     AALHG"
     gene            complement(30020..31132)
                     /locus_tag="VIO83_06560"
     CDS             complement(30020..31132)
                     /locus_tag="VIO83_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEY8330969.1"
                     /translation="MIKSRAAVAFAPNQPLQIVEVDVEAPKKGEVLVRIVATGVCHTD
                     AYTLSGQDSEGVFPCILGHEGGGIVEAVGEGVTSLQVGDHVIPLYTAECGECKFCKSG
                     KTNLCSAVRATQGKGLMPDGTSRFSYQGQPIYHYMGCSTFSEYTVLPEVSLAKIPKEA
                     PLEKVCLLGCGVTTGIGAVLNTAKVTEGATVAIFGLGGIGLAAIIGAKMAKASRIIGI
                     DINPAKEAVARELGLTDFVNPKDHAKPIQEVIVEMTDGGVDYSFECIGNVQLMRAALE
                     CCHKGWGESTIIGVAPAGAEISTRPFQLVTGRVWRGSAFGGVKGRSELPSYVEKAQKG
                     EIPLDTFITHTMGLEDINKAFDLMHEGKSIRTVIHF"
     gene            31295..32191
                     /locus_tag="VIO83_06565"
     CDS             31295..32191
                     /locus_tag="VIO83_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8330970.1"
                     /translation="MNRWEGLDEFVAVAECGQFTAAAEHLGVSSSHVSRQIARLEECL
                     QARLLYRSTRRVSLTEAGQTFLQHCRNLLDARDEAWRAVSDLSAEPKGLLRMTCGVAY
                     GERFVVPLVNEFMSLHPQLSVEIQLTNRTLDLLHEGLDLAIRLGRLGDERLVATRLAP
                     REMYLCAAPAYLARAGAPQSLAELARHNCLIGSSDVWTFADDGREQQLRVQGNWRCNS
                     GPAVLDAALRGFGLCQLPDYYVQEHLKSGALLALLEDHRPPHTAVWALYPPQRNLSPK
                     VRQLVDFLKLRLIELPEYRAAG"
     gene            complement(32197..32904)
                     /locus_tag="VIO83_06570"
     CDS             complement(32197..32904)
                     /locus_tag="VIO83_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEY8330971.1"
                     /translation="MDTRSLLDQLLKAAPGLLGSAGSQSKPRHAERTADKDSGLGSLL
                     SGAAGGGLAAGAIGLLLGNKKARKYGGKALTYGGLAALGVMAWKAYSNWQQQQGAVSP
                     SASESPQTVDRLPPQQAEQHSHAILQAVIAAAKADGHIDERERQLIDAEVAKLSQDPQ
                     LLQWVDRELHQPLDPAAVARAAQTPEMAAEMYLASLLLVDEQNFMERAYLDELARQLR
                     LDPALRGELDRQVQQAQ"
     gene            complement(32988..34094)
                     /locus_tag="VIO83_06575"
     CDS             complement(32988..34094)
                     /locus_tag="VIO83_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEY8330972.1"
                     /translation="MIQLFQPLTLRQLTLPNRIVVSPMCQYSARDGLANDWHLVHLGS
                     RATGGAGLIIFEATGVTPEGRISPQCLGLWSDEQIEPLRRITRFIDSQGSVAGIQLAH
                     AGRKAGTWRPWEGKPGSVPLEQGGWTPLAPSAIAFDPHHHVPQAMSREQIAALVQDFA
                     RAAERALKAGFKVVEVHAAHGYLLHQFLSPLSNQRDDEYGGSFANRSRLLLEVVDAVR
                     AVWPAELPLFVRLSATDWVDGGWSERETVELSAQLRERGVDLIDVSSGGLAANAQIPI
                     GPGYQARFAERVRREAGIPTGAVGLITEPAQAEHVLRCEQADLILLARELLRDPYWPL
                     HAAEALGHRLPWPPQYVRAAQRDTPIRELGEDLD"
     gene            complement(34257..35972)
                     /gene="recJ"
                     /locus_tag="VIO83_06580"
     CDS             complement(34257..35972)
                     /gene="recJ"
                     /locus_tag="VIO83_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY8330973.1"
                     /translation="MRIEPRPLPEILPELGDLPPLLTRLYAARGVRSASELDKALARL
                     IPYNQLKGIDRAVELLVEALDKRQRILFVGDFDADGATASTVGVLGLRQLGAAHVDYL
                     VPNRFEYGYGLTPEIVAVALQREPDLLVTVDNGISSVEGVAAAKAAGLRVLVTDHHLP
                     GHELPAADAIVNPNQPECAFPSKCMAGVGVIFYVLLALRARLRDLDWFTRNGWSEPNL
                     AELLDLVALGSVADVVPLDANNRILVHQGLARIRAGRARPGLRAILEVAGRDHRRITS
                     TDLGFILGPRLNAAGRLDDMSLGIECLLCEDEALARDMAQQLDQLNHDRKAIEQGMQR
                     EALAQLKDLQVDELPFGLCLFDADWHQGVIGILASRLKERYHRPTIAFADAGDGVLKG
                     SARSVPGFHIRDALDAVAARHPGLISKFGGHAMAAGLSLPQANFAAFAAAFDAEVRRQ
                     LVEDDLTGRLLTDGQLAADEFRLELAQSLRQAGPWGQHFPEPLFHGVFEIIQQRIVGE
                     RHLKLVLKSECGGAQLDAIAFNVDREVWPNPTVRWAEVAYKLDVNEYRGQESVQLLVA
                     HLEPR"
BASE COUNT         6392 a        12326 c        11617 g         5889 t
ORIGIN      
        1 caggccactc agcacggcac cgtcatcggc ccctgccaca cgcaggatca gggtcgcggt
       61 atcggtatcg ccgtcggcat cgcggatggt gtaggtgaag gtctcgctca ccacctctcc
      121 ggcattgagc gcctgggtcg ccgggagcgt gttgtcgagg acgaaactga aactgccgtc
      181 atcgccgagg accacggtgc cgtaggtcga caggatattt gcggtggaat cctcccaggc
      241 gacgaagaag cgcccgtccg cgccgatctg gccattggtg tgcacgtcat tgagcagcac
      301 gttgccctcg atcagactca ggccgtcctc gaccaggctg gccggtttgc cgctcaccgc
      361 attgggcacg tcgtcgagga tggtgacatc caggctctga ctggtcgtac tgccgccgct
      421 gccgaccagc tccaccgcga agctttcgct cagcgctgtg tcgttggccg gctgggtgtg
      481 catctcgttg tccagcaggg tgtagctgta gaacaccgtg ccaccgcccg tcgccggatc
      541 gtagtcgacc ccggtgatcg tcagcgtgtt gcccagcccg ctggtcaagg tcggcagcgt
      601 cccggccaac acgccaccgc tgatcaacgg gctcccgccc accgtcaggc tctgtacgcc
      661 atccggtgca ctgaaggtga agctgccggt ctgcgtcagc agcgccgcat tcgcggcact
      721 gccgtcggcg aggttggcat cgtcgacaac gatctcgcta cccgttccat ccaggccacc
      781 cagcacgacg ccgtctgcag tgccggtcac gctgagcgtc aggatcgcag tgtccgtatc
      841 gccatcggca tcgcggatgg tgtaggtgaa ggtctcgctc accacctctc cggcattgag
      901 cgcctgggtc gcagggcgcg tgttgtcgag gacgaagcgg tagctgccgt catccccgag
      961 taccacggtg ccataggtcg acaggatatt tgcggtggaa ttctcccaag cgacaaagaa
     1021 gcgtccgtct gcgccgatct ggccattggc gtgctcgtcg ttggacagca cattgccctc
     1081 gaccaggctc agaccgtcct cgaccaggct ggctgggacg tcgtcctccg cattgggcag
     1141 gtcgtcgagg atgtcgatgg tgatggtcgc cggtgccgaa ggagtcgtgc cgtcggacac
     1201 actcagggtg aacgtgtcgg tttcgatgcc ggcaaggtcg gtggtgggcg aggtcagctg
     1261 ataggtgtag gtggctacgc cggtagccgc gtcataggcc gtaatggtca gcgtaccgct
     1321 ggctccggca aagctggagc cagccaggct accgatggct accgtaatgc cgttgatggt
     1381 cacgctctgc aggtcgtcca gcccatcggc atccgacagg gtgaaggtgc cgctggcgaa
     1441 ttcgctgggc gacgcggcat ccgagccgct cgacaggccg gcttcgtcga ccacatcgtt
     1501 ggccccgccg ttccccggat ccaccgtcag actcggtacg tcgttgctgc ccgtgatggt
     1561 gatggtgact gtcgcagagc tttcgtcacc gtcagcgtcg cgcatggtgt aggcgaaggt
     1621 ttcggtcagg ctcgcccccg tatccagacc ctgcacgacc gggctggcgg tattcagggt
     1681 ataggtgtag gcgccgccgg cacccagaac cagttgtccg tagctgccga tagaggtccc
     1741 aggcgtggtg acgaccgctc cagcgtcggc accggcgatg tcgttaacca gtacgttgcc
     1801 gctaacgctg gtggcgtctt cgctcaggct gctgtcgtca ttgaccgccg taggcccgtc
     1861 atcgttgatc gcaatggtca gcgcctgcgg gcccgccgga tcgccttcgt tgtccgtggc
     1921 caccacgctg aagacatcga acagctgatc atcggtgccg gtcagattga cacccgcatg
     1981 atcgagactg ttgtcggtca gggtgtaggt ccagctgtat tcaccctcgc cgatcttggt
     2041 cacctgcagc acaccgtact ggccgtcgac tgcgccgccg ttggtcacat cgactccgcc
     2101 gatgatcacg ctgctgatgg tcacactgcc cagtgagtcg cccttggtat ccacaacaat
     2161 ggagccagag gtctgctcgg ccgtgctgga cggattgctg cccggcaagc catctccgct
     2221 accaccggca ttcaacgcct cctcgtcgac caccccggca ccggggccat aggtaatggc
     2281 cagatccgga acagagtcga cgcccacgcc aacggtcagc accgcctgcg aggtatcgcc
     2341 gtccgcatca aggatggtgt aggcgatcgt cgccaccccc tcgaagcccg gagcgggtga
     2401 gaaggtcacc gcaccatccg gcaggaagct tacagagcca ttgcctgagg tcagagaggc
     2461 accgatgaga gtcgctgtgt ccgagccctg gacatcattg accagcacat tgaccgttac
     2521 aggcgtgtcc tcgggagtca acacactgtc attgaccgcc gtcggcccgt catcgttgat
     2581 ggcgatggtc agcgcctgcg gccccaccgg atcgccttcg ttgtccgtgg ccaccacgct
     2641 gaagacatcg aacagctggt cagcggggcc ggtctggttg gcgccttcat gattgaggct
     2701 gttgtcggtc agggtgtagg tccagctgta ctcaccctcg gtgagcttgc tcacctgcag
     2761 cacgccgtac tggccgctga ccgtgccgcc gttggtcaca tcgaccccac cgatgaccac
     2821 gctgctgatg gtcacactgc caagtgagtc acctttggta tccaccacaa tcgaaccgga
     2881 ggtctgctcg gccgtactgg acggattgct gcccggcaag ccatctccgc taccaccggc
     2941 attcaacgcc gcctcgtcga ccaccccggc accagggcca taggtaatgg ccagatcagg
     3001 aacagagtcg acccccacgc caacggtcag caccgcctgc gaggtatcgc cgtccgcatc
     3061 ctgcatcacg tagctgatgg acgccaaacc ctcgaagccg ggcgccggag tgaaggtcac
     3121 cgagccatcc ggcaggaagc tgaccgtacc gctgccgccc gtcaggacgg ccgaaaccag
     3181 cgtggcgccg gagtcgacac cctggatgtc gttgcccagg acattgaccg tcaccggcgt
     3241 atcttcaggc gtcagcacac tgtcattgat cgcctgcgga ccgtcgtcgc caatgttgat
     3301 caccaaggat gccggtggcg aaatatcccc atctgcgtcg atcacgcgaa ccggaaaact
     3361 ttgcaccacc tcatccgtcg tggtggcatt ggggtccttg tgatcgaggg tattgccgtt
     3421 gagggtaaac acccaggtgc cattgggtgc gacttgcaag gtgccgtact gcccctggac
     3481 cacgccgccg tccaagacgt cgatccagtt gccgtccttg tcctggacct gaatggcttg
     3541 aatgccatcc agcgaagaaa tcaccacgcg accgaaggtc gactcggctg ggctttctgc
     3601 tcggctgccc cccggcagtg cagcctcgtc cacgacaccg cgctcggtca gaacctgccc
     3661 cgtggcatcg gcgaagtatt cgactcgaat cgaaggaatg ggatccagct cctcatccgc
     3721 cccctgcgga aagatctccg ggaatagcgg ggcaaactgc agcggtccgg tcggatagcc
     3781 gatgttgggg tccacgcggt cgccggtttc ccccagcatc acgaaggaaa taccgccacc
     3841 accactgccg gcggggccgc cagtgccggg gccggccgcc gtggcgggca ggctgagggt
     3901 gggatcgaca ccggaggcga tggcctgctg gacctcctcg acttcggcca gctcctgggc
     3961 gctgggcaca tgttcggggg tggcgggttg aatcggcgcg cgcgcatttt ctccggccag
     4021 cagctgggta tccagcatca gcgaactgtc acgccccacg gtcaactcgc cgccgttaac
     4081 caggcggatg gctacagcgc tcccggcacc agtgagcagt tgctcgccgg caaaaacccg
     4141 atccccctcg cccagttggc gcttgctgct gtccgcggct acggcaaaaa cctctccgac
     4201 aaccgccctg acaaatccga tcaacttagc catgaatcca ccctccgata cagtcgcaag
     4261 cgtcgactgt cgtgcgtcgc tgattgcccg gacaggacag aacagctgct accagggtgg
     4321 tgactgcaga ggtgtgtgtg ttagtgcgct ccgcctaccg cagagcaaat gacgaaaaac
     4381 cgtcactcca gtgaccactt actcccccct ccacaaagca gttcccccct gctttcgctc
     4441 tccctgccgc ccgatcttct gaagtctgca aaatccggct gaaaacggat ttccattggc
     4501 ttttctctct aaacaatgtg gcgcgttttc agcgacagat aagaccattt ctgcataacc
     4561 cttatgagaa atcgctctac gcaaaccatg gaggatgttc ttagatactt gagaacggaa
     4621 taaaaaaatc gccaggcagc agcagaagtc atttttttgg cgacaaccat tagcaagtgg
     4681 ataccaatgg taaggagata cccatgcgcc acacatcaat gtgggttggg ctgcttttgg
     4741 cagcaggttt caatcaagcc tcggccatgt ccctcacgga agcaattcag agcaccatcg
     4801 atagccaccc cgagatccat gcggccaaca acagccggtt gtctgctgac gaagaactca
     4861 agattgccca aggcggctac ctgccgacgg tcgacatgct ggccagctat gggcgggagc
     4921 aaaccgacag tcccagcacc cgcatcatcg gcgatcacaa cacggaaacc ctgaactccc
     4981 gcaccgcgga gctgcgtttg cggcagatgg tgttcgacgg tttctatacc cccaatgaag
     5041 tggcgcgcaa cgaagcggtg gtcaactcac gggcttaccg tgcgctgggc gccacggaaa
     5101 gcctggcgct gcgcaccatc gaggtctacc tggacgtgct caagcggcgc gagatggtgg
     5161 ccctggccga gaacaacctg caggcgcaca cgcgcatcca tgaccagatc agcatgcgtg
     5221 ccgagcgcgg ggttggcagt accgccgacc gcgaccagtc gcaggcgcgc ctggccttgg
     5281 ccgagaacaa cctctacacc gagcaggtga acctggccga tgccgaggcg aacttcttca
     5341 gcgccgtcgg ccgcatgccc gacgagctgg aaaccccggc ctcgatccgc ggccagatgc
     5401 cggaaagcgt ggccagcgcc cgcgaccggg tcatggacaa cagcccgctg ctcaagtcgg
     5461 cccagtccga cgtacaggcc gccgagaaac agtacgaatc ggccaagtcc aagttctatc
     5521 cacgcttcga tgtcgaactc tccggcaatg ccgacgacaa catccagggg gatgagggtc
     5581 attacaaccg ctggtatgcc ggcgtggtga tgaattacaa cctgttcaac ggcatgcgcg
     5641 acaaggccct gctgcagtcc ggcgcgcaca agatcaacga gtcgatggac atccgcaaca
     5701 acgccctgcg gatactcaac gagaacctga tgctgtcgtg gaacgccatg gaaaacgccc
     5761 gcctgcaaac cccgaaggcc cgtgattacg ccagctacac gtccagggtg cgcgaggcct
     5821 accagcagca gttttccctg gggcagcgca ccctgctcga cctgctcgac agcgaaaacg
     5881 aactgttctc cgccaatcgc cgctacaccg acgtgcgcta cacggaagag ttctcgatgt
     5941 accgggtagt ggcgtccatg ggcgacctgc tgcgcaccca caatgtcgtg ccgccgagcg
     6001 aggccctggc ccgcaccgaa gtgaaaagcg aggcgcgcct gccgagcatg aagtgacacg
     6061 ttccgcagtc cgcgcctgaa aataataaaa gcagggactt gcttgcagga tgggggatgc
     6121 ttcatgacga ccatggaaca ggtgggcaaa gccgatgacc ctcggcatat gcatgatgat
     6181 ccgctgctcg acggcctgct gattctcagt aggctgcacg gccgaacggt cagccgggcc
     6241 agtctcagcg ctggcctgcc gctgcccggg cagcgtcttt cccccgacct gctgacgcgt
     6301 gccgccgcgc gcgccggact gcaggcacgc ctgctgcgcc ggccgctgga ggctatttcc
     6361 gcccttaacc ttcccgtgct gctgctgttc aaggacggcc actgcgccgt actgcgccgg
     6421 tggaacgaca agggccaggc gcaaatcctg ccctgcgaag cagacggtgg cgagcagtgg
     6481 gtcgagcgcg aacaactggc tgccgaatac gccggacagg cactgttcgc tcgcccgcgc
     6541 catgagctgg aagagctgcg cacaccgctg gtcccccggg tgaagtcgtg gtttcgcgac
     6601 accctcaagc tgtcgcgcgg gctgtatacc gacgccatcc tcgccagcct gctgatcaac
     6661 ctgctcgggc tgatggtgcc cttgttcgtc atgcaaacct acgaccgcgt ggtccccaac
     6721 caggcgctct cgacgctctg ggtgctgacc atcggcctgc tgatcggcac cctgttcgag
     6781 ctgctgctgc gagtgctgcg cttccgcctg ctcgatgtgg ccggcaagaa gaccgatgtg
     6841 gtgctctccg ccaccctgtt cgaacgcatc accgggatga tgatgaagtc ccggccggcc
     6901 agcatcggcg gcttcgcgca gagcatccac gacttccagg gcctgcgcga attcctcacc
     6961 gccgtcaccc tgaccagtct gatcgacctg cccttcgccc tgctgatgac cgtggttatc
     7021 ggcctgctcg gtggctggct ggtggtcatt ccgctactcg ccttccccat caccgcgctg
     7081 ttcgcctacg tcatccagca ccgcctgcgc gacaccgtgc aacgcagcct ggccctgggg
     7141 gccgaacgcc aggcgctgct gatcgaaacc ctcggcggcc tggaaaccct gaagagctgc
     7201 ggtgccgaga gcgagcgtca gcaccagtgg gaaagcaccc acggcgccct gagccggctc
     7261 gagagtcacg cgcgcctgct gtccgccgtg gcgctcaacg gcaccctgtt cctgcagcaa
     7321 ctggccggcg tggcgatgat cgttgccggc gtctacagca tcatcgccgg caacctcagt
     7381 gtcggtgcgc tggtcgcctg ctacatgctc aatggccgga tcatggcacc gctcgggcag
     7441 gtcgccagcc tgatcacccg ccaccagcaa gcccagctga ccatgaaaag caccgacatg
     7501 ctgatgaacc tgccgcagga gcgcgacagc cagcagcggc cgctggagca cacccgcctg
     7561 cacggcgcac tggaactgcg ccaggtcgcc ttcagctatg cggagcagag ttcaccggcg
     7621 ctgcagggca tcaacctgcg catcgccccc ggcgagaaga tcgccatcat cggccgcagc
     7681 ggctcgggca agagcaccct ggcgcgcctg ctgatgggtt tctatagccc gagcgaaggg
     7741 cagatcctgt tcgacggcat cgaccagcgg caaatcgatg tcgccgacct gcgccaccag
     7801 atcggctacg tcgcccacga tctccccctg cttgccggca ccctgcgcag caacatgacg
     7861 ctgggtgccc gctacatcag cgacgaacgc ctgctcgaag tcgccgagct gaccggcgtc
     7921 agcgagctgg cccgccagca cccgcagggc ttcgagcggc cggtcggcga acgcggccag
     7981 ctgctctccg gcggccagcg ccaagcggtg ctactggccc gcgcgctgct gctcaatcca
     8041 ccgctgctgc tgctcgacga gcccaccagc tcgatggaca acagcagcga ggaagtgctg
     8101 cgccagcgcc tgcacgactg gagccgcagc aagaccctgc tcctgatcac ccaccgtact
     8161 tccatgctca gcctggtgga tcgtctgatc gtgctcgacg gcggcaagat cgttgccgac
     8221 gggccgaagg aaacggtcat ggaagccttg cgcaagggcc gtatcggccc ggcatcggtc
     8281 tagggagacg ctcatgagca ctctgcaaag cctgggtggt tacctgaacc cgctgcgtca
     8341 gtcccgcgat accgagttca tgccggaagt ccagggcgcg ttggtggagg actccaccgg
     8401 cctgacccgc ctgacggtct ggctgaccgc cctgttcctg ctgaccgcgc tggtgtgggc
     8461 ccatttcgcc gtgctcgacg aggtgaccac cggcgaaggc aaggcgattc cctcaagcaa
     8521 ggtgcagacc atccagaacc tggaaggcgg catcgtcgcg gagattttcg tccgcgaagg
     8581 ccaggtggtg gacaagggcg acccgctgct gcgtctggac gacacccgct tcgtgtccaa
     8641 catgagcgaa agcgaagccg atcgcctcgc cctgctcgcc cgggtcgagc gcctgagtgc
     8701 cgaaggcgaa ggccgcccgc tgcagctgcc ggaggagatc gccaaggtcg cgccgggcct
     8761 ggccgccgat gaggaggccc tgtaccgctc gcgccagcag gggctgagca gcgaggtcag
     8821 catcctgcag gagcaactgc ggcagaaaac ccaggagctg gcggagttcc gcgccaaggc
     8881 gcagcagtac cgctccagcc tcggcctgat ccaaagcgag ctgaacatgt ccgagccgct
     8941 ggtggccagc ggcgccatct cccaggtcga ggtgctgcgc ctgcgccgca gcgccgtgga
     9001 aacccgcggc tcgctgaatg ccaccaacct ggccattccg cgtgccgaat cgggagtcaa
     9061 ggaggttgag cgcaagatcg aggaagcccg cctgacgttc cgcaccaacg ccttgaagga
     9121 actcaacgaa gtgcgcaccg agctgagcaa gatcaccgct tccagtcgcg ccatcgagga
     9181 ccgggtcagc cgtaccggcg tggtctcccc ggtgcgcggc atcgtcaagc agctcaaggt
     9241 caacaccatc ggcggcgtgg tgcagcccgg cagcgacctg ctggaaatcg tgccgctgga
     9301 agacagcctg ctgatcgagg cccgcatccg cccgcaggat gtcgccttcc tccaccccgg
     9361 ccagcaggcg atggtcaagt tcaccgccta cgactacacc atctacggcg gcctcaaggc
     9421 caaactggag atgatcggcg ccgataccat caccgacgag gatggcaaga gcttctacct
     9481 gatccagctg cgcaccgaca aaagccacct gggcagcgtg gacaagccgc tgctgatcat
     9541 tcccggcatg gtggccaacg tcgacatcat caccggccag aagagcgtgc tcgactacct
     9601 gctcaagccg gtgctcaagg cgcgctcgga agcgctgcgc gagcgttgac cctcactcgg
     9661 ccggcgcatc accgcgcagg tgattgcgcc ggtagacctc ctcgcccatc gccgcaatct
     9721 gcatctcgat cagcgcctcg aacgggcgca gcaaagcatc gaaacgcccc gccccttcca
     9781 gcccgttcag gcaatgcacg atagcctcga tggtcgacag cgcaccggcc gccggggcct
     9841 tgcgcaggcg atagcgactg accagcccgt cctccagcgc cagccgcggc aaccctgcca
     9901 gccaggggtt gcagtagagg agacgccgcg ccttgcgcca ggtgccgtcc ggcacgatca
     9961 gcagccgctc gaccggctcg cccgcggcgg acgccgccaa ctgctcgacc gtctgggtgc
    10021 cttcgccggg aaacagcagc cacgccggca caccatccat cgccagttcg ctctccagct
    10081 ccgggaagtg ctcgccaatc cgcagctcgg cgttggctag cccgagcgcg gccagccgcg
    10141 cggtgttcag ggcgtggcgg gtctcgtccg ggtgctgcag gatcagcacc cgggtgtggc
    10201 tggctagatg ggggatcagc ggacacaggc agtgtgtcag cgggcgcgcg cagcgcgggc
    10261 attggactcg ggacatggcg cgcattttcg catgcgcggc ggagcgcgcc cagccatgcg
    10321 cctcagctca gcaagcggcg ccaccacgca tcggcactga ccaggctgat gccgaaaagc
    10381 accagcagac cgccaccgat aaacaaggcg tcgatgcgct cgcccagcag cagcacgccg
    10441 aagcagaccc cgaacagcgg ggtcatgaag gaaaacaccc ccagccccgt ggccaggtag
    10501 cggcgcagca gccagaacca caccagatag ctggcgaagg ccaccaccac gccctggaac
    10561 agcaggttgc cccacaccaa cggggtaagg cggaccacct ccgtctgccc gcccagccag
    10621 gcgagcggca gcagcagcag gcagccacag accagctgga acagcagggt cttggccgcc
    10681 ggcgcctcgg acagccgact gcagcgcacc gatacggtca gcgcgcccca cgacatgccc
    10741 gccagcagac cgtacagatc gcccagcagc atgccggccg acagctgctg ccccagcaac
    10801 ccgccggcga aggccaggat gattccggcg aaggccagca cgatgcccag ccactgcagc
    10861 cgccgcaggc gctcgctggg cacgcgccag tgcagcccca gcgcggcgaa gatcggcgcg
    10921 gtgtagagca ggaccacgat gtgcgcggca ctgctgtggc gcagcccctc ggcgacgaag
    10981 aagaactcca gggcgaacag cgtgccgacc agcagaccgg cgcgcagggt gccatcgccc
    11041 agggcgagcg gctggcggcg ccagagcatg aacagcgcca ccagcacggc ggcgatcccc
    11101 gagcgcaacc cgacctgcag cagcggcgcc atgtccggcg cggccagctt gatcccgacc
    11161 tgctgcagtc cccagatgcc gcacagcagc accatcagtt gcagggcgaa cgcatccacg
    11221 ccctggcgtg tggcagacat cgaaaactcc tgaaaaaacg gcaaaccgcc ggcaggaagc
    11281 cgcggggcag aatcggtcag ccggcgggag aggatagcga cagtgcctcc gcttcagccg
    11341 ctgcgccggc atacgggcgc ggacagtagc agccgcaacg caacgccgga agaggcagcg
    11401 ggacgcgctc agttggcagc cagaaagcgc atgccctctt ccagccccgc cggagtcaac
    11461 gggtacatgc gctcgtgcac cagctcgcgg accatgccgg tggaagccgt gtatttccag
    11521 gcatcctgcg ggtaggggtt gatccagatg agcttcttgt aggtgtccat gaaacgctgc
    11581 atccagacgt agcccggctc gtcgttccag tgctcgacgc tgccgccggc gtggctgatc
    11641 tcgtagggac ccatcgcggc gtcgcctacg aacaccacct tgtagtccgg gccgtacttg
    11701 tgcagcaggt cgagggtggc gaggcgctcg ccgtggcgac gctggttgtc cttccacacc
    11761 gtgtcataga cgaagttatg gaagtagaag tactcgagat gcttgaactc ggtgcggcac
    11821 gcggagaaca gctcctcgca gaccttgacg tggctatcca tggagccgcc gatgtccagc
    11881 agcaggagca gcttgaccgc attgcgccgc tcggggcgca tgcggatgtt gagcaggccg
    11941 gcgtcgcgcg cggtgtgctc gatggtgccg tcgaggtcga actcctcggc cgcgccctcg
    12001 cgggcgaact tgcgcaggcg gcgcagggcc agctgcaggt tgcgcgagcc cagctccatc
    12061 tggtcgtcga ggttgcggta ttcgcgctgc tcccagacct tggccgcgcg gccctgacgc
    12121 tggccggcat cgccgatgcg gataccctcg ggatgatagc caccggagcc gaacggactg
    12181 gtgccgccgg tgccgatcca cttgttgccg ccggcgtggc gctccttctg ctcggccagg
    12241 cgctgcttga acgcctcgag cagctggtcc agcccgccga ggctgttcag ctgggccttg
    12301 tcctccgcgc tgagcgagcg ctcgaactcc ttgcgcagcc agtcctcggg aatcagtgcc
    12361 tcgagcgccg catcgagact ctgcaggccg ttgaagtagg ccgcgaaggc gcgatcgaac
    12421 ttgtcgaaat gccgctcgtc cttcaccagc acggtgcgcg ccaggaaata gaaggcgtcc
    12481 atgtcggcga acaccacctg gcgctcgagc gctgcgagca ggtcgagcag ttcgcgcagc
    12541 gacaccggca ccttggccgc gcgcatttcg ttgaacaggt tgagcagcat ctctatgccc
    12601 ccgggaatga ccgtagtgtg ggcaaccgcg cagcgttgcc caccggtgga aaaccgcctg
    12661 gcgggttttc ccgccctacc gcccggcgcg ccgactcatg aatgccaggc gctcgagcag
    12721 ctgcacgtcc tgctcgttct tcaccagcgc gccggccagc ggcgggatgg ccttgcccgg
    12781 gtcgcgctcg cgcagcagcg cctcgccgat ctggtcggcc atcagcagct tgagccagtc
    12841 caccagttcg gaggtggacg gcttcttctt cagccccggc accttgcgga tgtcgaagaa
    12901 gatttccagc gcctcctgca ccagctcctg gcggatgccg gggaagtgca cgtcgacgat
    12961 gcgctgcagg gtggcgcagt cggggaaggc gatgtagtgg aagaaacagc ggcgcaggaa
    13021 ggcgtccggc agctccttct cgttgttgga ggtaatcagg atgatcggcc gctgcgccgc
    13081 cttgatggtc tcgccggtct cgtagacgaa gaactccatg cggtcgagtt cctgcaacag
    13141 gtcgttgggg aactcgatgt cggccttgtc gatctcgtcg atcagcagca ccacccgctc
    13201 gccggcctcg aaggcctccc acagcttgcc cttgcgaatg tagttgcgca cgtcgtggac
    13261 cttgtcgacg cccagctgcg agtcgcgcag acgactcacc gcgtcgtact cgtagaggcc
    13321 ctggtgggcc ttggtggtcg acttgatgtg ccaggtgatc agccgggtgc ccagcgcctc
    13381 ggccagctgt tcggcgagca gggtcttgcc ggtgcccggc tcgcccttga ccagcagcgg
    13441 gcgctgcagg gtgatggcgg cgttgaccgc cagcttgaga tcgtcggtgg cgacgtagga
    13501 ggcggtgcct tcgaacttca tcggtatcaa tccttgcgcg gacaggcatg gccggccctg
    13561 cgggcggcga atgcccgact atagcgtgcc ggcccgggcg caggaaacgg cggtgctgga
    13621 cgctcccgcc acgaccgcat aggctgagcc ggttcatctt ttcatcggag gagaagtccc
    13681 gaccatgagc cgcatctacg cagacaacgc gcaagccatc ggcaatacgc cgctgatccg
    13741 catcaaccgc ctcggcccgc agggcgtgac catcctggcc aagaacgagg gccgcaaccc
    13801 ggcctactcg gtcaagtgcc ggatcggcgc cagcatgatc tgggacgccg agcagagcgg
    13861 ccggctcaag ccgggcatga ccatcgtcga accgacctcc ggcaacaccg gtatcggcct
    13921 ggccttcgtc gccgccgcca agggctacaa gctggtgctg accatgcccg cctcgatgag
    13981 cctggagcgg cgcaaggtgc tcaaggggct cggcgccgag ctggtgctga ccgagccggc
    14041 caagggcatg aagggcgcca tcgacaaggc cagcgaactg gccgccgccc acccgggcga
    14101 atacctgctg ctgcagcagt tcgagaaccc ggccaacccg gcgatccacg agcagaccac
    14161 cggcccggag atctggaacg ataccgacgg cgccatcgac gtgctggtgg ccggcgtcgg
    14221 caccggcggc accatcaccg gcatctcgcg ctacatcaag aacaccatgg gcaagccgct
    14281 cctctcggtg gcggtggagc cggccggctc gccagtgatc agccagaccc tggccggcgc
    14341 cgaggtcaag cccagcccgc acaagatcca gggcatcggc gccggcttcg tgccgaagaa
    14401 cctcgacctg tcgatcgtcg accgtgtcga gacggtgagc gacgacgagg caaaggagat
    14461 ggccctgcgc ctgatgcgcg aggaaggcat cctctgcggc atctcctgcg gcgccgccat
    14521 ggccgccgcg gtgcgcatcg ccaaggagcc gcagatgcag ggcaagacca tcgtggtgat
    14581 cctccccgac tccggcgagc gctacctgtc gaccatgctg ttcgacggcc tgttcggcga
    14641 gcaggagctg cagcagtaac cgccccggcc agaggagcga catgttcccc tggcaccgcc
    14701 cggacagccc ggcaaaagcc ggccaacccg cgccacgtct gggcttgaag ggatttttcg
    14761 cggcatggcg aaaaagtgtt tgccatgccg gacgggaacg ggcaaagtga aggtgtaggt
    14821 caaggcggaa tacgtaagct gtgaacgcac tgattcagta cgtcagttcg tcattctcga
    14881 tatcttgatt ccacaccgat gaactccggt tcagctacaa atcaacacct aggaattaca
    14941 cgaaatggca actggcactg ttaagtggtt caacgacacc aaaggcttcg gtttcatcaa
    15001 gccggacgac ggtggtgacg acctgttcgc ccacttctct gaaattcgcg ctcagggctt
    15061 caagaccctg gccgaaaacc agaaagtgtc cttcgaagtg accactggtc cgaagggcaa
    15121 gcaagcttcc aacatccaga tcatcggcta atcgctgatc tgtttgtgaa aaaaacccgg
    15181 ctctcgagcc gggttttttt atgcccgcga ttcagcgcaa gcccctagcg ctggttccac
    15241 cgttcgcaaa ccccgcctca gccggcgacc tgtccgcacg ccgcgacttc gccccgccgc
    15301 cagagcatcc actgccccgg cagatacagc gcccaggcct cgttggcggt cagcggctcg
    15361 gtagcgacca ccgtcaccac gtcgtccgga gtggtttccg cctggaagtc cacgcgtaga
    15421 tcggcatcct tgagctgcgc cggcccgaaa ggcgcacgcc gggtaatgtg tgccagcttg
    15481 gtcgagcaga agctgaacag ccagtcgccg tcgctgagca gggcattgaa cacgcccagg
    15541 cggcgatagt cggcgcaggc ctggaccagg gtcggcagca gctgcgcgat ctccaccggt
    15601 tcgggaaagt ccagacggac gcgattgagc agatcgcaga aggccgcttc actgtcggta
    15661 tcgcccaccg gccggtagaa accgggctgt ggcgcaaaac ccgccaactg gccgttgtgc
    15721 gcgaagcacc agttgcgccc ccacagctcg cgcacgaacg gatgggtgtt ggctaggccg
    15781 accttgccga cgttggcctg gcggatatgg ccgatgacca cctcgctctt gatcggatag
    15841 cgctgcacca gccgcgccac ctccgactcg ctgctcgcct gcggatcctg gaacaggcgc
    15901 aggccacggc cttcgtagaa ggcgatgccc cagccgtcgc gatgcgggcc ggtgcggccg
    15961 ccccgctgca tcagtccggt gaagctgaag acgatatcgg tcggcacgtt ggcgctcatg
    16021 cccagcagtt cacacatgcg acgactccct caggacggcg ggcttacagg cgcggctcga
    16081 cgcgaccgtc gtccagattg cgctgctcga gcacttcacg catggtcggt tcgcgcgccg
    16141 cctcggcggc ctgacgcttc gcccggctgc ggcgcagcag cggccagcgg atgacgacga
    16201 acagcaggta gagggcaaaa ccgatcatgc cgaacatcgc caggtcgctg atcgcccgcc
    16261 acaggttgcc gccggtcttc atcagcagat ccagtgcggt gatggcgatc gccggggcat
    16321 agaactcgcg caccggatcg accagcgtcg ggctcagcag cagcacggcc atcagtaccc
    16381 gcaagggctc gcgcagcccg cgccacatcc agcgggtgat gcgaaagccg accagcaggc
    16441 agcccagcgc ggcaacggcg tagacggccc aggcgagaag gtaatcttgt tcagtcatcg
    16501 gtgcgtggcg gcagcggaat aagggcgctt atgatagcgg cttttccccg gagccgcgcg
    16561 tcagctgcgg ctggagtcag ccgatgtccc tcaccccgcc cctcgcccgc cgccaggatg
    16621 gcgccgatcc ctatgcctgg ctggagcagc gcgacagcgc cgaagtgctc gactacctgc
    16681 atgccgagaa tgcctggctg gaacagcagc tcgccgacca gggggaactt cgcgagcagc
    16741 tgttcgagga gatccgcgcg cgcatccgcg agaccgacct gtcgctgccc acgccctggg
    16801 gaccgtggct gtactatcag cgcaccacgg ccggcgagga gtatccgcgc cactaccgct
    16861 gcccgcgccc ggccgacggc tcgctgggca tcgacgaagc cggcgagcag ctgctgctcg
    16921 accccaacct gctggccggc gacggctacc tggacctcgg cgccttcagc atcagtcccg
    16981 accaccgccg cctggcctac agtctggaca ccgagggcga cgagatctac cagctgtacg
    17041 tcaaggaact gggcacgggc gaagtcatcg ccctgccctt cgccgactgc gacggcagca
    17101 tgacctgggc caacgacagt cggacgctgt tcttcggcga gctggacgac acccatcgcc
    17161 cgcacaagct gtatcgccat cgcctgggcg agaaggatgc cgaactggtg ttcgaggagc
    17221 aggacggccg cttcttcctc ggctgccacc gctccagctc cgagcgccag ctgatcctgc
    17281 tgctggccag caagaccacc agcgaggcct gggtgctcga cgccgagcaa ccgctgggcg
    17341 agttccgctg cctggccccc cgcgcggaag accacgagta ctaccccgac cacggccagg
    17401 tcgatggcca gtggtgctgg ctgatccgca gcaaccaggc cggcatcaac ttcgccctgt
    17461 accgtgccga ggaaagcgcg ccgacccgcg agcactggca ggtgctggtg ccgcaccgcg
    17521 aggcgctgat gctcgaggac ttcagcctca acgccggcgc catcaccctc gacctgcgcg
    17581 acaacggcct gccggtgatc gaggtgctgc cccgcggcgc cgcgagctac cgggtgcaac
    17641 tgccggatgc ggcctacagc ctgcatgtct acgacgacct ggagttcgac agcccggcga
    17701 tccgcctgcg ctacgaggcg ctcaaccgtc cggcgcagat ccgccagctg gagctggcca
    17761 gcggcacgca gacggtgctc aagcagaccc cggtggaagg cccgttcgat gcggacgcct
    17821 acgagagccg ccgcgtctgg gccaccgcgg cggacggcac tcaggtgccg atcagcctgg
    17881 tcggccgccg cgacagtttc ggccgccccg cgccgctcta cctgtacggc tatggcgcct
    17941 acggcgaaag cctcgacccc tggttctccc acgcgcgcct gagcctgctg gagcgcggct
    18001 tcctgttcgc catcgcccat gtgcgcggtg gcggcgagct gggcgaggcc tggtaccgcg
    18061 ccggcaagct ggagcacaag cagaacacct tcggcgactt catcgcctgt gccgaaaagc
    18121 tgatcgccga cggctacacc accccggcgc agctggcgat cagcggcggc agcgccggcg
    18181 gcctgctgat cggcgccgtg ctcaaccagc gccccgagct gttcgccgcg gccatcgccg
    18241 aggtgccctt cgtcgacatc ctcaacacca tgcagaaccc cgagctgcca ctgacggtga
    18301 ccgagtacga cgagtggggc gacccgagcg agccggcggt gtacgagcgg atccgcgcct
    18361 atgcgcccta cgagaacgtc agcgcccagg cctacccggc gatcctcgcc gtcgccggct
    18421 acaacgacag ccgcgtgcag tactgggagg cggccaagtg ggtggccaag ctgcgcgcca
    18481 gcaagaccga cgccaatctg ctgctgctca agaccgagct gggcgccggc cacgtcggca
    18541 tgagcggccg ctatcaggca ctcaaggatg cggcgctgga atacgccttc ctgctcaaag
    18601 tgttcggcct ggcctgaagc ccgaccgatc aacgacaaag ggcgcccgtc ggcgcccttt
    18661 gtcgttactg ccaaccccgc tcagcgcagc aggcgggtat ccagcacctt cgacggcccg
    18721 cccaggaggt tctcgctgac ctgcacgaag tcccgcgtgc tggccttgtc caggcgcatc
    18781 aggccgcggg tcacatcatc cagcgagtgc cggttgccgc tggcctgacg aatctcggcg
    18841 tcgaggtcct gcagcagcag cacggcccgc gccgtcaccg gaccgctggc ctgctcgccg
    18901 cgcagggacg tgaccttgcg actccagcgg gtcaactgct cgcggacctt ctggtagcga
    18961 tcctcgctca ggccaccggc gcgacgcatc agctcgatgg cgtagtactc ggcaatgcct
    19021 tcggtgatcc agtcgctgcg gtcggtatcg ctgatgcggc tgaacacatg gaccagttca
    19081 tgcaccagcg agctggtgcc gttttcactg accagcggac ggtcgcggtg catgtacagc
    19141 gaattgggcg cggacagacc gccgcgccac atcgggtcgc cggcgccgac gatcagcagc
    19201 ttggccggct cgcggggaaa caccgcctgg gcgtgcggcc agacgaaagt cagcagggtg
    19261 aggatgtcca tgcgccgcac gccctcgccc accggcgcgg ccaccgccac gtcggtcttg
    19321 cccagggtag cgcgccgggt gccgagcttg ccggccagga tccagccggt ggggcggtcg
    19381 aacagccgct ctggattgtc gatgcggaaa cgattcttgc cgacccgtgg ccagccggtt
    19441 tccacgctct gccagccctc gggcagctcg aactgcaggc gcgaaaccag ctcgacgtcg
    19501 tcctgcacat cgatcttggc cgccggcacc agatcgtcgc cgcgcagcag cgcccagtcc
    19561 ggggtcatgc gggcgtcgaa gcggccattg gcacgcgggt ggctgatctt cacccggtag
    19621 gtcagctgcg ccttgccttc ctgcgggcgc cagaccccgt gctcgggact gctctgctgc
    19681 cactcgccct gggcctggaa gtcgctgtaa tagcctttgt cgccgaggct gaagtccagg
    19741 tacctcagcg cttggccgcg ttccagggtg agggtgacct cggcctggtc gctgtccggc
    19801 aggaagcgca cccgataatc cacgtcgacc cgctgcgccg cagccagcgg cgtggcaagc
    19861 ccgagcagca gcaccgccca cggtgtcttg aagcgttgga acatgacgga atctccttat
    19921 gctgggcagt tggataacct gtgcgcagac aggttcagcc ggcacgaaaa atcaggtgct
    19981 cttcccagtc ttcctcggcc acgctggact cggcgagcat gcgcccggcc tgggaaatgc
    20041 gcacatcgtg caccgcctgt ggatcgccgc agaccagatg gtgccagggc agcagatcct
    20101 tgccttcggc caccaggcga taggcgcagg tcggcggcag ccagcggaac tctgcggcat
    20161 cggcaggggt gagctggatg cagtcgggaa catgctgctt gcgctcggcg tagttgctgc
    20221 agcggcaggt gtcccggtcg aggagcttgc aggcgatgcg tgtgtagtag acgctgccat
    20281 cctcctcatc ctcgagtttc tgcaggcagc acaggccgca accatcgcag agcgactccc
    20341 attcgtcgcg atcgagctga acgagtgtct tgcgcttcca gaagggttcg gtcttggcgg
    20401 ccatggcggg gtctccgtga gggtgcggca gtctagccgc ctgcctcggc gcagccaagt
    20461 gcaggctgga gaccgctgcg accggcggcg cggaggccgc cgccggtcgc cactggctat
    20521 ttgcccatgc cggcgcgctg ggccttgagc tgcttgagca tcatttcgac gtgctcgatc
    20581 tgccggtcga gctgttcggc ggtcggcgca gcgcccatcg cgcccgaccc catgccgtga
    20641 cccatgcccc catccatcat ccccggcccc atgcccatgc ccataccgcc gtgcgcaccg
    20701 gcggccgggc agccgccgga catgcccatg ccgcccgcca tcatcgcctg acggtgctcc
    20761 agcatcagct tctgccgttc ctcgggtgtc ttcgccccgt gaatggccaa gcattgctgc
    20821 tgcatcaact gacaatgctg ctgaacctca ctgccgcccg caccgggtga cgatggcgtc
    20881 tgcacacagg ctgccagact caccagtaac ccacccaaca gcaaccgacc tatcttgcct
    20941 gcgctcatgt tgcttcctct tgcctatgcc tcgaagcagt tacgatagaa cgccgcgacc
    21001 gcttgtgctt gaccccgatc aagtcggcgc ccagcgcgga cgccttccac acgcggatgc
    21061 ggaacaagcg cacgggactc gctggcggca aacccaagac gccgccagct cggcaggctc
    21121 agaccggatc gttgcggcgc agcagttcgt ccggcaggtg ctcgatatag tcgtcctccg
    21181 gcggcggcat ctgcaggtgg tagccctgct tctcgatgtt ctccagcacc tgggccgcat
    21241 cctcgcgggc cagcttgcgc tcgggggtga gcagcatgtc gaacacgtgc tgcggcgcgc
    21301 cgaaggcgcc gagcaggcct tccggcacgc gcttcagggc ctcgcgcttg tcgacgtaca
    21361 ggtacatctc gcgccgcggg cttttgtaga tggagcagat acgtttcatc gtcaacctca
    21421 agccagggta tcgagcaggg cctggcccat cagcgcgcgg cgccagccgc gcagggaatc
    21481 cggcagggtg taggggccgg cgggatagcc ggttttcagc aaagcctcaa gcaccttctt
    21541 gcgcagcatc agctcgggca cgatctgcag gcgctcgccc tcgcgctggc cgaccgcgcg
    21601 cagcttcttc aacagtgccg aggcttccag cggcagcggc tcgggcagct gcgccggcca
    21661 atgctcggcc ggcagggccg ccgcctcggc aatcagccgc agcagggttt cgccgtcctg
    21721 acgcacggtc cgcgggtgca tgtcttcgat gcgcgccaac gccaccacat cgcgcggctg
    21781 ggtacgcgcc agcggccaca acgaacgctc gcgcaggatg cggttgcgcg gctggtcgcg
    21841 ccggcgcgcc tgctcctcgc gccaggtgca gagtgcacgc agcacggcga gctgctggcc
    21901 attcaggcgc caggccagct tgacctcgcg ccaggcctct tgcggatcgc tcacctcacg
    21961 ctgggcagcc accaactcgg cgccgtcgtc cagcagccag gcacgcttgt cagcactcag
    22021 tcgcgcctcc agttcacggt agacctcgca caggtgctcc acgtcctcgg cggcatagcg
    22081 cacctgcatc tcgctgagcg gacgctgcaa ccaatcggaa cgggtctcgt ccttcggcag
    22141 gtcgaggccc agcagctcct tgaccagccg cgagtagccc atggaaaaac cgatgttcag
    22201 gtagccggcg gcaatctggg tatcgaacag cggctgcggc aggctgccgg tcaggcgctt
    22261 gaacacctcg agatcctcgc tgcaggagtg cagcaccttg accactgccg gatcctccag
    22321 cagggcggca aagggtgccc agtcgcggat ctgcagcgga tcaatcagcc acaccgcctc
    22381 accatcgctc acctgcacca gcgcggcgat cggatagaag gtgtcgaccc gcatgaattc
    22441 ggtatccagg gcaacgaacg accgcgtgcg ccagacactg cagcaggcag cgaggctggc
    22501 gtcgtccagg atccagcgga tatcgatagc gggcacgggg ctctcctgcg gaaaaagtgc
    22561 agtataccgc gcaagcctgg cgaacagctg cgaatcgggc ctgcgcggcg ttcccggccg
    22621 cggtaatctt tagccgttta ccggcgctcc ccaagacaac ccatttgccg aggtcccccg
    22681 atgtcgctcg cccgcctgat cagcccgcaa gacctggccc gccgcctggg gcagcccggc
    22741 ctggtgattc tcgactgccg cttcgccctg gaggacccgg cgtatggccg gcgcgcccac
    22801 gctgccgagc atctgcccgg cgcctgcttt gccgatctgg agcaggacct gtcggcaccg
    22861 gtgatagccg gcaagaccgg ccgccacccg ctgccggatc cgcagcagct gtgccagcgt
    22921 ctgcgcgagt ggggagcgag caacgacagc gaaatcgtgc tgtacgacga cggccccggt
    22981 gcctttgccg cccgcgcctg gtggctgctg ctgtggctgg gcaagcgcga aggtgtctac
    23041 ctgctggatg ggggattgag cgcctggcag ggtgccggac tgccgttgga gcaacatcag
    23101 cctcaacccg agcccggcga cttcgcgggt agcccggacg acagcctggt gctgggcggc
    23161 gacgaattgc tgcagcgcct gagcgacccc gagctgatcc tgctggatgc gcggggattg
    23221 ccgcgctttc gtggcgaggt ggaaccgatc gacccggtgg ccgggcatat tcccggcgca
    23281 cagtgcgcag ccttcatcga caacctgggc aacgacggcc gcttcctgcc agccgaagtc
    23341 ctgcggcggc gctttgcagg gctgctcggc atgcgtccgc cggaacaact ggtcgcctac
    23401 tgcggttccg gggtaaccgc ctgccacaac ctgttcgccc tgtgcctcgc gggctacccg
    23461 ctggccaggt tgtatgccgg ctcctggagc gagtggatta ccgatccggc caggccggtg
    23521 gcgcgcgata gctgagcacg cacaatcctc ctgtggcggc cagcctggcg gcgggaacaa
    23581 cgccggcaaa aaacgcagac aaaaaaaagg gcgggttacc ccgccactct ttttttccct
    23641 aatcaccgtc ctggtgaacc acttccagtg gtgtcctgac cggcatccgt gccggctgcg
    23701 tcattccctg tacgcaaggt agatattagc cgtggcgggg ttttgcacaa gcacgcggcc
    23761 agccgctttc cactcgggaa aaacgcctca ccaaaaaatt aatcctttaa aaacaatcaa
    23821 ttaaaaaacc ataaaaaatt aaaggataaa ttccggcaaa caccccagag atcatcctac
    23881 aagacatgta agcaatgact tacatgtcct gtaggcgaac cgccggaact ttctcaaagc
    23941 aaggtagcca cgcgctcctt tatccgtatg cccacaagca cgaaccccga ccgaggccgg
    24001 ggttcgcatg cagggctgga gcagaacgcg ctttattgaa agagcgactc gctggacagg
    24061 ccgttgcttt ccagaatctc gcgcaggcgc ttgagcgcct cgacctggat ctgccgtacc
    24121 cgctcccggg tcaagccgat ctcacggccg acctcctcca gcgtgctgct ttcgtgcccc
    24181 cgcaggccga agcggcggat caccacctct cgctgcttat cggtcagctc ggacagccac
    24241 aggtcgatgc tctgccccag gtcctcatcc tgcagcagtt cgcagggatc ggtcggatga
    24301 tcgtcggtca gggtatccag caaggtcttg tcggagtccg gaccgagggc gacgtccacc
    24361 gaggtcaccc gctcgttcag gcccagcatg cgcttgacct cggaaaccgg cttttccagc
    24421 aacgtggcga tttcatcggc ggtgggttcg tgatcgagct tctgcgtcag ctcacgggcg
    24481 gcacgcagat agacgttgag ctccttgacc acatgaatcg gcaggcggat ggtgcgcgtc
    24541 tggttcatga tcgcccgttc gatggtctgg cgaatccacc aggtggcgta ggtggagaag
    24601 cggaaaccgc gctcgggatc gaacttctct accgcgcgga tcaggccgag gttaccctcc
    24661 tctatcaggt cgagcagggt caggccgcga ttgacgtaac gccgcgcgat cttcaccacc
    24721 agacgcaagt tgctctcgat catgcgcttg cgcccagccg cttcgccgcg acgggccatg
    24781 cgcgcgaaat gcacttcctc ttccgggctg agcaacggcg agaaaccgat ttcgttgagg
    24841 tagagctggg tggcatccag tgcgcgggtg tagtcgacaa acttgtgctg ccgcgcactg
    24901 ctggaagagg tggtgctagc gacggactca acctcgtcgt cgacattcaa agagagcatt
    24961 tccggagcga cacccagctc catcaggagc acttcatcgt cacggtcgaa ttccggcgtt
    25021 gtttttgtag ttgccatttt ttctcggtcc ctttctgagc tcgacgacag gcttttgcac
    25081 gcaatctatt gcttggtcaa agcccgctta ctcacactat caggacgaaa tgacagctca
    25141 atcaacgact cggcagatat tgcaacggat cgacgggttt accctgacgg cgaatctcga
    25201 aatgcagctt cacacgatca gtacctgtcg cccccatttc cgcaatctta tctccagcct
    25261 tgacctgctg cccctcccga accaataact tgcgattatg tccgtaagca ctgacgtagg
    25321 tatcgttatg cttgatgatg acaagctcac catacccacg aagtccactg cccgcatata
    25381 ccacgcttcc tccggaagct gcgagaacag gctggcccaa ttcaccgtcg atatcaacgc
    25441 ctttattcaa actgctgttc gaggaaaaca aactgatcaa ttggccattc gccggccatg
    25501 tccagcccgc ggcggaccgg gcggcctgcg cctcaccggc attgccggcc gcagccgcgg
    25561 ataccggtgc gacggtctgc ggccgagtcg cgaccgggtt gccgccacgc gcctgcaccg
    25621 ctgctggcgg cggcgtcgtg gccgccatgg ggactgcagg cccactgccg ccaaagcgaa
    25681 tcacctggcc gacgcggatg gtgtagggcg ccgggatatt gttgcgcacg gccagcgcct
    25741 tccagtccca gccgtagcgg aaggcgatcg agtacagggt gtcgccacgc cgtacccggt
    25801 actggccgtg ggtcaccggc tgacgcgagg agccggcacc atgctggctg ccgatagtct
    25861 cgctggtacc gctcgatgag gcgcaaccgc ccagcagacc cagcgacagg ctcaggcaca
    25921 ggccgcccag cagccgcggc atccaattcg atatgtgccc cacctggccc agctccttat
    25981 tgctcgcgcg gattgtttcg ttcatggcca cagttgcagg aaggtatttc gcatcgggtt
    26041 tttccgtaaa acagcagtac gactcagaat accgagccac tcaacagcgg gacgaaacgc
    26101 accgggtcca gcacttggcg ggagaaacgc tcgccctcgc gcacgatcag catcagctgc
    26161 tgctcttcgc cggttcctac cggaatcacc agccggccgc cgggcgccag ctgatccagc
    26221 agagcctgcg gcacctgtgc ggcggcagcg gtgacgatga tgccgttata cggggtcaag
    26281 gccggccagc cttcccaacc gtcaccccac cgaaagacca cattgcgcag attgagttcg
    26341 agcaggcgtt ccttggcacg atcctgcagc gactggatgc gttcgacgga aaacacccgc
    26401 tcgaccagct gagccagcac ggcggtctgg taaccggagc cggtgccgat ctccatcacc
    26461 ttgtccagcg ggccgccagc cagcagcagc tcggtcatgc gcgcgaccat gaacggctgg
    26521 gaaatggtct ggttgtggcc gatcggcagc gcggtgtcct cgtaggcccg gtgcgccagc
    26581 gcctcgtcga cgaacaggtg gcgcggcgtg cgacgaatca cctcgaggac ctgggcattg
    26641 ctcaggccct cttcgcacag gcgctggatc aggcgctcgc gggtacgctg cgaagtcatg
    26701 ccaatccccc gacgctgcag atcatccctt atcacagcag attctccaga atgctcaggc
    26761 cacggaaggc ttcgtgaaag gtgcgatcca gctgcagcgg cgtgatcgac acgtagccct
    26821 gcatcactgc atggaaatcg gtccccggcc cgccatcctc ggcatcgccg gccaccgaga
    26881 tccagtagcc ttccttgccg cgcggattga ccgacttgac cggtgccggc gcgcggccac
    26941 gatggccgag gcgggtcagc tgcacgccgc ggatgcgctc cagcggcagg ttgggaatgt
    27001 tgacgttgat cacggtgcgc ggcggcagct cgagctggtc gtgcatcgcc accaggcgcc
    27061 gggcgatgtg cgcggccgcc ggcaggttgt cgggctggcg cgacagcagc gagaaggcga
    27121 atgccggctt gtcgaggaaa cgcccctcca gggcggcagc cacggtgcca gagtacagca
    27181 cgtcgtcgcc cagattggcc cccaggttga tccccgacac caccatgtcc gggatgtgct
    27241 cgagcaggcc gttgatcccc aggtgcacgc agtcggtcgg tgtaccgttg aggctgatga
    27301 agccgttgtc cagccgctgc ggatgcaggg gacggtcgag ggtcaacgag ctgcttgccc
    27361 cgctgcggtc agcgtcgggc gcgatcacca cgcactcggc gtaatccgcc agcgcgttgt
    27421 gcagcgcgac tatcccgggg gcatacacgc cgtcatcgtt ggcgatcaat attcgcatgg
    27481 ggtgtccaca ggtcctgttg gcagcagccg gacgagttca cgcaccagca cggtggcgaa
    27541 acagccggcc ggcaggacga attccagttg caggatgtca gtggcaggat aatgccacgt
    27601 cagcccgccg atggggaggc gcagcacgcg tcgcgcctgc tccagaccgg catctgccag
    27661 ccagttcacc agtacggcat cgtccacctg gacctgctgc tcgagtactc gcacggcgcc
    27721 gccggtttcc agctcgccag caccgaacag cggcccagtg ggatgcaggt ccagcgccgc
    27781 cagacgcgga tcgctgcact cggcctcgcc ggccgggaaa aagctgcggc tgtcggtgaa
    27841 cgccagcagg tcgccgggca gcgcgcggtt ccagctgcca tcggccacgc gcgcggccag
    27901 cacgcggttg aacaggtagc tgcgcgccgc cgacagcacc cgcgagcggc ggttgcgctg
    27961 ctccggcagg gtcttgcgct ccgcccagtg gcgcgcctcg tgcaggttgc caccgtcgaa
    28021 accgaagcgc tgcagaccga agtagttggg gatgccggcg ccggcgatct gctgcagacg
    28081 ctgctccagc gcctcgcgct cggcctgcag cgcggtcagg cggatgatga agccgttggc
    28141 ggcatgggca ccgcgctgca acttgcgcga gtgacgcgcg cgctcgagga tgcgcaggtc
    28201 gtcgccttcg gcggcggcca gatcgggatc ggccttgccc ggcaggtgca ggctgaacca
    28261 ctggcgggtc agcgcctggc gatccttgag accggcatag ctgatcgcct tgagcggcac
    28321 cccggcggca cgggcgatgc gccgcgccgc atcctcggtg ttcagcccgc gcttctccac
    28381 ccacagccac aggtgctcgc cggcaccgga cagcgggata tccagcacct cgttgacctg
    28441 gaagtcctcc gcggtgtgct tgagcacggc ctggccgcag ggctcgcccc aggcggtgag
    28501 gccgagcagg tcgagttcgc cgagtacgct actcatcgcg ccagcaacag ggcgactgca
    28561 tgcacgacga tgccctcctc gcggccggtg aagccaagct tctcggtggt ggtggccttg
    28621 acgttgacct ggtccagcac gatgccgagg tcggcggcga tggtggcgcg catcgcctcg
    28681 atgtgcgggg ccatcttcgg cgcctgggca acgatggtgg cgtcgacgtt gccgacctgc
    28741 cagcccttgg cctgcaccag ggcgaacacg tggcgcagca gcacgcggct gtcggcgccc
    28801 ttgaactgcg gatcggtatc cgggaagtgc cggccgatgt cgcccagcgc cgccgcgccg
    28861 agcaacgcgt cgctgagcgc atgcagcagc acgtcgccgt cggaatgggc gaccagcccg
    28921 aatctgtggg gaatgcgcac gccgccgagg gtgatgaact cgccctcgcc gaagcggtgt
    28981 acgtcgtagc cgtggccgat acgcatatag aagaacgccc tggaaaagtc agggcgtgat
    29041 tctacaggac tggccagcgt gtaggagagc ggctcagccg tgcagcgccg ccgcatgatg
    29101 acgcaggtga tcgtcgatga aactggcgat gaagtagtag ctgtggtcgt agcccggctg
    29161 caggcgcagg gtcagcggat gaccgacggc ggcggccgcg gcacgcaata cctctggctt
    29221 gagctggatg gccaggaagc tgtcggcctc gccctggtcg accaggatcg gcaggcgctt
    29281 gctggcggtt gccagcagcg cgcaggcatc ccactgccgc cagctgtcac gatcgctgcc
    29341 gagatagcgg gaaaacgcct tctcgcccca gggacagctg ctcggattgc cgatcggcgc
    29401 gaaggccgac agcgagcggt agcgccccgg attgcgcagc gcgcagacca gcgcgccatg
    29461 gccgcccatc gagtggccgc tgatgccgcg ccggtcgctc accgggaagc tggcctcgac
    29521 cagcgccggc agctcgtgca ccacgtagtc gtgcatgcgg tagtgctgtg cccaaggctg
    29581 ctcggtggcg ttcagataga agccggcgcc gtggccgaaa tcccaggcgc cgtccggatc
    29641 gcccggcacc tcggcgccac gcgggctggt atccggcgcc accagcacca accccagctc
    29701 ggcggccagc cgctgcgcgc cggccttctg catgaagttc tcgtcggtgc aggtcagccc
    29761 gctcagccag tacagcaccg gcagtttcgc gcccggctct gcctgcggcg gcaggtagac
    29821 ggcgaacacc atctcgcagt tcagcgtggc cgaacggtga cggtagcggt tgtgccagcc
    29881 gccgaagctc ttgttgctgg aaatgatttc gagggtcatg aaccactccg tagggtgcgc
    29941 cgtgcgcacc catgggtttc ggtgcgcacg gcgcacccta cgagagaccc gcggggccag
    30001 agaacctcgc ggttcagctt cagaagtgga tgacggtacg gatgctcttg ccctcgtgca
    30061 tcaggtcgaa ggccttgttg atgtcctcca gacccatggt gtgggtgatg aaggtatcca
    30121 gcgggatttc gcccttctgc gccttttcca cgtagctcgg cagctcgctg cgacccttga
    30181 cgccgccgaa ggcgctgccg cgccagacgc gaccggtgac cagctggaac ggccgggtgc
    30241 tgatctcggc gccggccggc gccacgccga tgatggtcga ctcgccccaa cccttgtggc
    30301 agcactccag cgccgcgcgc atcagctgca cgttgccgat gcactcgaag gaatagtcca
    30361 cgccgccgtc ggtcatctcg acgatcacct cctggatcgg cttggcgtgg tccttggggt
    30421 tgacgaagtc ggtgaggccc agctcgcggg ccacggcctc cttggccggg ttgatgtcga
    30481 tgccgatgat gcgcgaagcc ttggccatct tggcgccgat gatcgccgcc aggccgatgc
    30541 cgcccaggcc gaagatcgcc acggtggcgc cttcggtgac cttggcggtg ttgagcaccg
    30601 caccgatgcc ggtggtcacg ccgcagccga gcaggcagac cttctccagc ggcgcctcct
    30661 tggggatctt ggccagggag acttccggca gcacggtgta ctcggaaaag gtcgagcacc
    30721 ccatgtagtg gtagatcggc tggccctggt agctgaaacg gctggtgccg tccggcatca
    30781 ggcccttgcc ctgggtggcg cgcaccgcgc tgcacaggtt ggtcttgccg gacttgcaga
    30841 acttgcactc gccgcactcg gcggtatata gcgggatcac gtggtcgccc acctgcagcg
    30901 aggtgacgcc ctcgcccacc gcttcgacga taccgccgcc ctcgtggccg agaatgcacg
    30961 ggaacacgcc ttcggagtcc tgccccgaca gggtataggc atcggtatgg cacacgccgg
    31021 tggcgacgat gcgcaccagc acctcgccct tcttcggcgc ctccacatcc acctcgacga
    31081 tttgcagcgg ctggttggga gcaaaggcta cggcggcacg cgacttgatc atgcggaact
    31141 cctggaaacg ataagggcca tgcgcggcga aaaccaaagc gccacgccac aacgaatggc
    31201 acaaagtgta gatcagcaca gaacaacgag taatcgacgc ccaggcaaaa tattattgct
    31261 gtacggggat aatcccacca cccaaggagc cgccatgaac cgctgggaag gactggacga
    31321 attcgtcgcc gtggccgagt gcggccagtt cacagctgcc gccgaacacc tcggcgtctc
    31381 ctcctcgcat gtcagccggc agatcgctcg cctcgaggag tgcctgcagg cccgcctgct
    31441 gtatcgcagc acccggcggg tcagcctgac cgaggccggg cagacctttc tgcagcactg
    31501 ccgcaacctg ctggacgccc gcgacgaggc ctggcgcgcg gtcagcgatc tgtccgcgga
    31561 gcccaagggc ctgctacgca tgacctgcgg ggtggcctac ggcgagcgct tcgtggtgcc
    31621 gctggtcaac gagttcatga gcctgcatcc gcagctgagc gtggagatcc agctgaccaa
    31681 ccggaccctg gacctgctgc acgagggcct cgacctggcc atccgcctcg gtcgcttggg
    31741 cgacgagcga ctggtggcga cccgcctggc gccgcgggag atgtacctgt gcgcggcgcc
    31801 cgcctacctc gcgcgcgccg gcgcaccgca gagcctggcc gagctggcgc ggcacaactg
    31861 cctgatcggc agcagcgatg tgtggacctt cgccgacgac ggccgggaac agcagctgcg
    31921 cgtgcagggc aactggcgct gcaacagcgg ccccgcggtg ctcgacgccg ccctgcgcgg
    31981 cttcggcctg tgccagctgc ccgactacta tgtgcaggaa cacctcaaga gcggcgcgct
    32041 gctcgccttg ctcgaggatc accggccgcc gcacaccgcg gtgtgggcac tgtatccgcc
    32101 gcagcgcaac ctctccccca aggtgcgcca gctggtggac ttcctcaagt tgcgcctgat
    32161 cgagctgccg gagtaccgcg ccgctggctg atgccgttac tgcgcctgct gcacctggcg
    32221 gtccagctcg ccgcgcagcg ccgggtcgag gcgtagctgg cgagccagtt cgtccaggta
    32281 ggcgcgctcc atgaagttct gctcgtccac cagcagcagg ctggccaggt acatttccgc
    32341 cgccatctcc ggggtctgcg ccgcgcgggc caccgccgcc gggtccagcg gctggtgcag
    32401 ctcgcggtcg acccactgca gcaactgcgg atcctggctg agcttggcca cctcggcgtc
    32461 gatcagctgg cgctcgcgct cgtcgatatg gccatcggcc ttggccgcgg cgatcaccgc
    32521 ctgcaggatc gcatgactgt gctgctcggc ctgctgcggc ggcaggcggt cgacggtctg
    32581 cggcgactcg ctggctgacg ggctcacggc gccctgttgc tgctgccagt tgctgtaggc
    32641 cttccaggcc atcaccccga gggcggccag gccgccgtag gtcagggcct tgccaccgta
    32701 tttgcgggct ttcttgttgc ccagcagcag gcctatggcg ccggccgcca gccctccgcc
    32761 ggccgcgccc gacaacaggc tgcccagccc gctgtccttg tcggcggtgc gctcagcgtg
    32821 gcgcggtttc gattgactgc cagcgctgcc gagcaggccg ggggcggcct tgagcaactg
    32881 gtcgagcaga ctgcgggtat ccatgggtca cgctcctgtg aagatcggcc gattatgccg
    32941 ccgccacggc ggcaggcggc cctggaaact gcaagcgcat gtaacgctca gtccagatcc
    33001 tcccccagct cgcggatcgg cgtgtcgcgc tgggcggcgc gcacgtactg cggtggccag
    33061 ggcagtcgat ggccgagcgc ctcggcggca tgcagcggcc agtaggggtc gcgcagcagc
    33121 tcgcgggcca gcaggatcag atcggcctgc tcgcaacgca gcacatgctc ggcctgcgcc
    33181 ggctcggtga tcagccccac cgcccccgtc ggaatgccgg cctcgcggcg cacgcgctcg
    33241 gcgaagcgtg cctgatagcc cggcccgatc gggatctgcg cgttggccgc caggccgccg
    33301 gaggacacgt cgatcaggtc gacgccacgc tcgcgcagct gcgcgctcag ctcgacggtt
    33361 tcccgctcgc tccagccgcc gtccacccag tcggtggcgg acaggcgcac gaacagcggc
    33421 agctcggccg gccacactgc gcgcaccgca tcgaccacct ccagcagcag acggctacgg
    33481 ttggcgaagc tgccaccgta ctcgtcgtcg cgctggttgc tcagcggcga gaggaactgg
    33541 tgcagcaggt agccgtgggc cgcatgcacc tcgaccacct tgaagccggc cttgagggcg
    33601 cgctcggcgg cgcgggcgaa gtcctgcacc agcgcggcga tctgctcgcg gctcatggcc
    33661 tgcgggacgt gatggtgcgg gtcgaaggcg atggccgacg gcgccagtgg cgtccagccc
    33721 ccctgttcca gcggcaccga gcccggcttg ccttcccacg gccgccaggt gccggccttg
    33781 cgcccggcat gcgccagctg gatgccggcc accgagccct ggctgtcgat gaagcgggtg
    33841 atgcgccgca gcggctcgat ctgctcgtcg gaccacaggc ccaggcactg cgggctgatc
    33901 cgcccttcgg gcgtgacgcc ggtggcctcg aagatgatca gccccgcgcc accggtggca
    33961 cgactgccca ggtgcaccag atgccagtcg ttggccaggc catcgcgggc gctgtactgg
    34021 cacatcggcg agaccacgat gcggttgggc agggtcagtt gccgcaaagt cagcggctgg
    34081 aacagctgga tcatggcgag gctccgcaag tcgtccgtta tcttgcggat attgcgccag
    34141 tggcggggag aatcaagggg cgcgcccgtc gcgccccgat ccagaccgca gcaggagacg
    34201 gcgcgtgcaa gggagggttc ccgcgcaggc gcgtggaacc ctcagcgggg caacggtcag
    34261 cgtggctcga gatgcgccac cagcagctgc acgctttcct ggccgcggta ctcgttgacg
    34321 tccagcttgt aggccacctc ggcccagcgc acggtcggat tgggccagac ctcgcggtcg
    34381 acgttgaagg cgatggcgtc cagctgggcg ccgccgcatt cgctcttcag caccagcttg
    34441 agatggcgct cgccgacgat gcgctgctgg atgatctcga atacgccatg gaacagcggc
    34501 tcggggaagt gctggcccca ggggccggcc tggcgcagcg actgggccag ctcgaggcgg
    34561 aactcgtcgg ccgccagctg gccgtcggtg agcaggcgac cggtgaggtc gtcctccacc
    34621 agctggcggc gcacctcggc atcgaaggcg gcggcgaagg cagcgaagtt cgcctgcggc
    34681 agcgacaggc cggcggccat ggcgtggccg ccgaacttgc tgatcagccc cggatggcgc
    34741 gcggccaccg catccagcgc atcgcggatg tggaagcccg gcaccgagcg cgccgagccc
    34801 ttgagcacgc cgtcgccggc atcggcgaag gcgatggtcg ggcggtggta gcgctccttg
    34861 agccgcgagg cgaggatgcc gatcacgccc tggtgccagt cggcgtcgaa caggcacagg
    34921 ccgaacggca attcgtccac ctgcaggtcc ttgagctggg ccagcgcctc gcgctgcatg
    34981 ccctgctcga tggccttgcg gtcgtggttg agctggtcga gctgctgggc catgtcgcgg
    35041 gccagcgcct cgtcctcgca gagcaggcac tcgatgccca ggctcatatc atccagccgc
    35101 cccgcggcgt tcaagcgcgg gccgaggatg aagcccagat cggtagaagt gatgcgccga
    35161 tgatcgcgcc cggcgacttc gagaatcgcc cgcaaccccg gccgcgccct cccggcacga
    35221 atccgcgcca gtccctgatg cacgaggatg cggttgttgg cgtccagcgg caccacgtcg
    35281 gcgacgctgc ccagcgccac cagatcgagc agttcggcca gattcggctc gctccagccg
    35341 ttgcgcgtga accagtccag gtcgcgcagg cgcgcgcgca gtgccagcag cacatagaag
    35401 atcaccccca caccggccat gcatttgctc gggaacgcgc attcgggctg gttcggattg
    35461 acgatcgcat cggccgccgg cagctcgtga cccggcaggt gatggtcggt caccagcacc
    35521 cggagcccgg ctgccttggc cgccgcgacg ccttcgacgc tggagatgcc gttgtccacg
    35581 gtcaccagca gatcgggctc gcgctgtagc gccacggcga cgatttccgg ggtgaggccg
    35641 tagccgtact cgaagcgatt gggcaccaga tagtcgacat gcgccgcgcc cagctggcgc
    35701 aggccaagca cgccgaccgt gctcgcagtg gcgccatcgg catcgaaatc accaacgaag
    35761 aggatgcgct ggcgcttatc cagcgcctcg accagcaact cgactgcacg gtcgatgccc
    35821 ttgagctggt tgtagggaat cagccgcgct agcgccttat ccagctcgct ggccgagcgc
    35881 acgccgcgcg ccgcatacag acgagtcagc agcggcggca gatcgccgag ttcgggcagg
    35941 atttcaggta aagggcgggg ttcgatgcgc atcggctggc tttccggtca cggctcttcg
    36001 gcacgggcgc cggagaaaat cggcgcaaaa gcatacagca atgcaccgtc agcaggccgc
    36061 caatcgcgat tcagccgacc ggtgccgttc aacgctcgcc cagcagccac tccagcgaca
    36121 cctcgaactg gccacgctcg tcggtgatga acagggtgcc gtcgctgatc atcacgcccc
    36181 attgcagact gcgcggcaga tccttgacca gtgcggcaag cgat
//
LOCUS       DATCUN010000160         4773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_44556, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000160 DATCUN010000000
VERSION     DATCUN010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44556"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(307..2091)
                     /locus_tag="VIO83_06585"
     CDS             complement(307..2091)
                     /locus_tag="VIO83_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent methanol/ethanol family
                     dehydrogenase"
                     /protein_id="HEY8330974.1"
                     /translation="MKHSGLTHTFARTTLGLALALSGMSAWAVTDDDILKDAQTPGEV
                     VTNGLGLQGQRYSPMDTLNADNVTQLRPVWAFSFGGEKQRGQQAQPLVKDGVMYMTAS
                     YSRVFAVDARTGKELWQYDARLPDGIMPCCDVINRGVALYDNLVIFGTLDAKLVALDK
                     DTGKVVWKKTVADYKAGYSITAAPLVVNGKLITGVSGGEFGVVGKIEAYNPKNGELLW
                     TRPTVEGHMGYVYKDGKPVEHGITGGEAGKSWPGDLWKTGGAAPWLGGYYDPETNQLL
                     FGTGNPSPWNSHLRPGDNLYSSSRLALNPDDGTIKWHFQSTPHDGWDYDGVNELVSFN
                     YQEGGKTIKAAGTADRNGFFYVLDRTNGKFIRGFPFVDKITWAKGLDKDGRPIYNEDN
                     RPGKPGDAGAQGKTVTVAPSFLGGKNWMPMAYSQDTGLFYVPSNEWSMDIWNEGTAYK
                     KGAAYLGAGFTIHPLNEDYIGVLRAIDPKTGKEVWRYKNYAPLWGGVLATKGNLVFTG
                     NPEGFLMAFDAKTGKKLYEFNTGSGVIGSPITWEQDGEQYVSVLSGWGGAVPLWGGEV
                     AKRIKDFNQGGMVWTFKLPKEVAAVAKR"
     gene            complement(2310..3317)
                     /locus_tag="VIO83_06590"
     CDS             complement(2310..3317)
                     /locus_tag="VIO83_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY8330975.1"
                     /translation="MQVIRLQAPGGLDRFQLLAEQDPGQPGAGEIRVRVHASSLNYHD
                     YLIASGAWPTVDGRIPMSDAGGVVEAVGAGVSEFAVGDHVLSCFFPRWQDGPPTVSDF
                     AEVPGDGWDGYAREVVVCPASWFTRAPQGYSHAEAATLTTAGLTAWRALVVNGGLKAG
                     DSVLVLGSGGVSIFALQFAKAMGATVLATSSSDAKLARMRELGADHCINYRQTPEWGA
                     VVRELTGGRGVDHVIEVGGPGTLAQSIAAVRTGGHIALIGVLTGHAGEVPTTLLMAKQ
                     ARVQGLIVGSRREQVDMVRAIDAIGLRPVIDRRFALADIGAAFRHQESGAHLGKICLE
                     F"
     gene            complement(3384..4322)
                     /locus_tag="VIO83_06595"
     CDS             complement(3384..4322)
                     /locus_tag="VIO83_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     1"
                     /protein_id="HEY8330976.1"
                     /translation="MRLFSLLVATILSLPLAAAELAYELEPRQIAADTWLLEGSTENF
                     AASNGGNIVNVAFIVTDAGVVLIDTGISKRYGEALRAAIGRVTDKPIALAINTHHHPD
                     HVFGNQAFSDVPIASLAHTRELLAEQGGAFADNLYRLLGDWMRGTEVLLPGETLEPGV
                     REIGGHRLQLLAFSGHTGADLAIFDQTTGVLFAADLVFYQRALTTPQTPGLDVWLADL
                     DALEKLPYTLLVPGHGPVATGHEPFVQMRDYLGWLDGLLRAAVAEGQDMNEAMRAPIP
                     ERFAAISQARFELTRTVSHLYPKYEARVFQRVDRQD"
     gene            complement(4440..>4773)
                     /locus_tag="VIO83_06600"
     CDS             complement(4440..>4773)
                     /locus_tag="VIO83_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEY8330977.1"
                     /translation="GCTAPSAVRAQPGWEEHLGEVFGARFPQGAGSRLRLRIAHPMDN
                     GLSGGIPEFYIERAELRGAAGAVLASLELHPSVSENPTLTLQLDGQDAGQQLWLRDNN
                     GNEFEAGF"
BASE COUNT          700 a         1609 c         1664 g          800 t
ORIGIN      
        1 cgatgaagtt gccgtagcgc ggcttgaggg aaaggatggc gccagcggta cccggttgtg
       61 cgtagatcat gctcatggcc tctcttttat tgttgcggtc cgatctggtt ctgcgcggac
      121 tgtgggttga tggtgtcagc cgcgctgccc tgcgggtatc acgcgaaggg ctacagaatc
      181 tcgttatttg gtagtagagc cgagtgagcc tagaagctac ccgccgccgt ggcgggcgtc
      241 cagcggcaca aatgcgaagg ggtcggcacc tggccgaccc cttctgctgg catcaccccc
      301 tgacgatcag cgcttggcga ccgccgccac ttccttcggc agcttgaagg tccacaccat
      361 gccgccctgg ttgaagtcct tgatgcgctt ggctacctcg ccgccccaca gcggcaccgc
      421 accaccccag ccggacagca ccgagacgta ctgctcgccg tcctgctccc aggtgatcgg
      481 cgagccgatc acgcccgagc cggtgttgaa ctcgtagagc ttcttgccgg tcttggcgtc
      541 gaaggccatc aggaagcctt ccgggttgcc ggtgaacacc aggttgccct tggtggccag
      601 cacgccgccc cacagcggcg cgtagttctt gtagcgccag acttccttgc cggtcttcgg
      661 gtcgatggcg cgcagcacgc cgatgtagtc ctcgttgagc gggtggatgg tgaagccggc
      721 accgaggtag gccgcgccct tcttgtaggc ggtgccctcg ttccagatgt ccatgctcca
      781 ctcgttggag ggcacgtaga acagcccggt gtcctggctg taggccatcg gcatccagtt
      841 cttgccgccg aggaacgacg gcgccacggt caccgtcttg ccctgcgcgc cggcatcgcc
      901 cggcttgccc ggacggttgt cctcgttgta gatcggccgg ccgtccttat ccaggccctt
      961 ggcccaggtg atcttgtcga cgaacgggaa gccgcggatg aacttgccgt tggtgcggtc
     1021 gaggacgtag aagaagccgt tacggtcggc ggtgccggcc gccttgatgg tcttgccgcc
     1081 ttcctggtag ttgaacgaca ccagctcgtt gacgccgtcg tagtcccaac cgtcgtgcgg
     1141 ggtggactgg aagtgccact tgatggtgcc gtcgtccggg ttcagtgcca gacgcgagga
     1201 cgagtagagg ttgtcgcccg ggcgcaggtg cgagttccac ggcgacgggt tgccggtacc
     1261 gaacagcagc tggttggttt ccgggtcgta gtagccgccc agccagggcg cggcgccacc
     1321 agtcttccac aggtcgcccg gccaggactt gccggcctcg ccgccggtga tgccgtgctc
     1381 gaccggcttg ccgtccttgt agacgtagcc catgtggcct tccacggtcg gccgggtcca
     1441 cagcagttcg ccgttcttcg ggttgtaggc ctcgatcttg ccgaccacgc cgaactcgcc
     1501 gccggacacg ccggtgatca gcttgccgtt gaccaccagc ggcgccgcgg tgatcgagta
     1561 gccggccttg tagtcggcca cggtcttctt ccacaccacc ttgccggtgt ccttgtcgag
     1621 cgccaccagc ttggcgtcga gggtgccgaa gatcaccagg ttgtcgtaca gcgccacgcc
     1681 gcggttgatc acgtcgcagc acggcatgat gccgtcgggc aggcgcgcgt cgtactgcca
     1741 cagctccttg ccagtgcgcg catccaccgc gaacacccgc gagtaggagg cggtcatgta
     1801 catcacgcca tccttgacca gcggctgggc ctgctggccg cgctgcttct cgccgccgaa
     1861 ggagaacgcc cacaccggac gcagctgggt gacgttgtcg gcgttgaggg tgtccatggg
     1921 gctgtagcgc tggccctgca ggccgagacc gttggtgacc acctcgccgg gggtctgcgc
     1981 atccttgagg atgtcgtcgt cggtgacggc ccaggccgac atgccggaca gtgccagcgc
     2041 gaggcccaag gtggtcctag cgaaagtatg ggtgagaccg gagtgcttca tacggctacc
     2101 tctgcgggtt cattgttatt gccgggaatt tttcccggtc tgcagcgaat tcttcgccgc
     2161 agggggtggc gcaacaattg atcgcgggga gcaaagacat gcttccttgg tagcaatacc
     2221 cagcgtcgca agggcgcgcc gggcgccgga agtcagcgca acgcaggaag tccaggggcg
     2281 cgggaggttg ctcccgcgcc ggcagggact cagaactcga ggcagatctt gccgaggtgg
     2341 gcaccgcttt cctggtggcg gaacgccgcg ccgatgtccg ccagggcgaa gcggcggtcg
     2401 atcaccggac gcaggccgat ggcgtcgatg gcgcgcacca tgtcgacctg ctcgcggcgg
     2461 ctgccgacga tcagcccctg cacgcgcgcc tgcttggcca tcagcagggt ggtcggcacc
     2521 tcgccggcat ggccggtcag cacgccgatc agggcgatat gcccgccggt gcgcacggcg
     2581 gcgatcgact gcgccagggt gcccgggccg ccgacctcga tgacgtggtc gacgccgcgc
     2641 ccgccggtca gctcgcgcac caccgcgccc cactcgggcg tctgccggta gttgatgcaa
     2701 tggtcggcgc ccagctcgcg catgcgcgcc agcttggcgt cggacgacga ggtggccagc
     2761 acggtggcgc ccatggcctt ggcgaactgc agggcgaaga tcgacacccc gccgctgccg
     2821 agcaccagca cgctgtcgcc ggccttgagg ccgccgttga ccaccagcgc gcgccaggcg
     2881 gtcagcccgg cggtggtcag ggtcgccgcc tcggcatggc tgtagccctg cggcgcgcgg
     2941 gtgaaccagc tcgccgggca caccaccacc tcacgggcgt agccgtccca gccatcgccg
     3001 ggcacctcgg cgaagtcgct caccgtcggc gggccgtcct gccagcgcgg gaagaagcag
     3061 gacagcacgt gatcgcccac cgcgaactcg ctgacgcccg cgcccaccgc ctcgaccacg
     3121 ccgccggcat ccgacatcgg gatgcggccg tcgacggtcg gccaggcccc gctggcgatc
     3181 aggtagtcgt ggtagttgag cgagctggcg tgcacccgca cgcggatctc gcccgccccg
     3241 ggctgccccg ggtcctgctc tgcgagcagt tggaaacggt cgagaccgcc gggcgcctgg
     3301 agtctgatga cctgcatggg gagttctcct gagggaatgg cgaagaagag gagcggcccg
     3361 catcgcgcgg gccgcgcagc agcttaatcc tgccggtcga ccctctggaa cacccgcgcc
     3421 tcgtatttcg ggtacaggtg gctgaccgta cgggtcagct cgaagcgggc ctggctgatg
     3481 gcggcgaagc gctcggggat cggcgcgcgc atcgcctcgt tcatgtcctg gccctcggcc
     3541 actgccgcgc gcagcaggcc gtcgagccag ccgaggtagt cgcgcatctg cacgaagggt
     3601 tcgtggccgg tcgccaccgg gccgtggccg ggcaccagca gcgtgtacgg cagtttctcc
     3661 agcgcatcca gatcggccag ccagacgtcc aggcccggcg tctgcggggt ggtcagcgcg
     3721 cgctggtaga acaccaggtc ggcggcgaac agcacgccgg tggtctggtc gaagatcgcc
     3781 aggtcggcgc cggtatggcc gctgaaggcc agcagctgca ggcggtggcc gccgatctcg
     3841 cgcacgccgg gctcgagggt ctcgccgggc agcagcacct cggtgccgcg catccagtcg
     3901 ccgagcaggc ggtacaggtt gtcggcgaag gcgccgccct gttcggccag cagctcgcgg
     3961 gtatgcgcca gcgacgcgat gggcacatcg ctgaaggcct ggttgccgaa cacgtggtcg
     4021 gggtggtggt gggtgttgat cgccagcgcg atgggcttgt cggtgacccg gccgatcgcc
     4081 gcgcgcaggg cttcgccgta acgcttcgag atgccggtgt cgatcagcac cacgccggcg
     4141 tcggtgacga tgaacgccac gttgacgatg ttgccgccgt tgctcgccgc gaagttctcc
     4201 gtgctgcctt ccagcagcca ggtgtccgcg gcgatctgcc ggggctcgag ctcgtaggcc
     4261 agctcggccg ccgccagcgg caggctgagg atagtcgcca caagcaggct gaacagacgc
     4321 atggtgcctc ctttccggtt gatcggtgtg ctgggtgcgc acggcgcacc ctacccacgc
     4381 agcgtcctga gccggatggt gcgccgcgcg caccatccgg caccacgagg actggccact
     4441 cagaaccccg cctcgaactc gttgccgttg ttgtcgcgca gccacagctg ctggccggcg
     4501 tcctgcccgt ccagctgcag ggtcagggtc gggttctcgc tgaccgaggg atgcagctcc
     4561 aggctggcca gcaccgcgcc ggccgcgccg cgcagctcgg cgcgctcgat gtagaactcg
     4621 gggatgccgc cggagaggcc gttgtccatc gggtgggcga tgcgcaggcg caggcggctg
     4681 ccggcgccct gcgggaagcg cgcgccgaac acctcgccga ggtgttcctc ccaaccgggc
     4741 tgggcgcgta ccgcactcgg cgcggtgcag ccg
//
LOCUS       DATCUN010000161         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_62703, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000161 DATCUN010000000
VERSION     DATCUN010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62703"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..929
                     /gene="phbB"
                     /locus_tag="VIO83_06605"
     CDS             180..929
                     /gene="phbB"
                     /locus_tag="VIO83_06605"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017338797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEY8330978.1"
                     /translation="MAAKQRVVLVTGGMGGLGETISTKMVDAGYQVVVTYSPSNTKST
                     EWVAAMKKNGYEITAVACDVADFDSCTKAVAEVQSKVGAVDILVNNAGITRDTTFKKM
                     DKVNWDAVMRTNLDSLFNMTKQVADGMVERGWGRVINISSVNGSKGAFGQTNYSAAKA
                     GVHGFTKALALEVAKKNVTVNTISPGYIGTKMVTAIPEDVLNTKILPQIPIGRLGKPE
                     EVAGLVIYLASEEAAFVTGANIAINGGQHMQ"
     gene            970..>1509
                     /locus_tag="VIO83_06610"
     CDS             970..>1509
                     /locus_tag="VIO83_06610"
                     /inference="COORDINATES: protein motif:HMM:NF042805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6781 family protein"
                     /protein_id="HEY8330979.1"
                     /translation="MIKAGIDQDAIVKMFSEATAQQGETLRRAVSEATLKALQGRELT
                     MANIKKVLSTVTTAATAGAAQNVASPIDVEALLTKAFSGMDAALLQAVEANRKALQQF
                     VDQGAGLQEKQLKGALANIEKMEDTFFATVTKAALGVAGPMQGPWEHVLGAMKLQGTD
                     TGAQAAQTVEQLMAQTQTAL"
BASE COUNT          305 a          495 c          512 g          197 t
ORIGIN      
        1 ggcggcagtg agcgtgtcca cccgccaatc taggcgccgg cccgcgcggc ggccttgatc
       61 ccggtcaagc gccgattcgc cgccgcgcct gtggaacccc tcttgcgcgg cccggcacgc
      121 gccggtaaca ctcgtccgaa cggcgcacct ccgttttcaa tcaacaagga gatacaacga
      181 tggcagcaaa gcaacgcgtg gtcctggtca ccggcggcat gggcgggctg ggcgagacga
      241 tctcgaccaa gatggtcgat gccggctacc aggtcgtcgt gacctactcg ccgagcaaca
      301 ccaagtcgac cgaatgggtc gccgcgatga agaagaacgg ctacgagatc accgcggtgg
      361 cctgcgatgt ggccgacttc gactcctgca ccaaggccgt ggccgaggtg cagagcaagg
      421 tgggcgcggt cgacatcctg gtcaacaacg ccggcatcac ccgcgacacc acgttcaaga
      481 agatggacaa ggtcaactgg gacgcggtga tgcgcaccaa cctcgacagc ctgttcaaca
      541 tgaccaagca ggtcgccgac ggcatggtcg agcgcggctg gggccgggtg atcaacatct
      601 cgtcggtcaa cggcagcaag ggtgcgttcg gccagaccaa ctactcggcg gccaaggccg
      661 gcgtgcacgg cttcaccaag gcgctggcac tcgaggtcgc gaagaagaac gtcaccgtca
      721 acaccatctc gccgggctac atcggcacca agatggtcac cgcgatcccc gaggacgtgc
      781 tgaacaccaa gatcctgccg cagatcccga tcggccgcct cggcaagccc gaagaggtcg
      841 ccggtctggt gatctacctg gcgtccgagg aagcggcctt cgtcacgggc gcgaacatcg
      901 cgatcaacgg cggccagcac atgcagtgag cgcgcggcgc ttcccttcac ccactctcga
      961 ggagattcaa tgatcaaggc agggatcgac caggacgcga tcgtcaagat gttctccgag
     1021 gccaccgccc agcagggcga gacgctgcgc cgggccgtca gcgaggcgac gctgaaggcg
     1081 ttgcagggcc gcgagctgac gatggcgaac atcaagaagg tgctgtcgac cgtgacgacc
     1141 gcggcgaccg ccggcgcggc gcagaacgtc gccagcccga tcgacgtgga agcgctgctg
     1201 accaaggcgt tcagcggcat ggacgcggcg ctgctgcagg ccgtcgaggc caaccgcaag
     1261 gcgctgcagc agttcgtcga ccagggcgcg gggctgcagg agaagcagct caagggcgcg
     1321 ctggccaaca tcgagaagat ggaggacacc tttttcgcca cggtgaccaa ggccgcgctg
     1381 ggcgtggccg gcccgatgca gggcccctgg gagcacgtgc tgggcgcgat gaagctgcag
     1441 ggcaccgaca ccggcgccca ggccgcgcag accgtggagc agctgatggc gcagacgcag
     1501 accgcgctg
//
LOCUS       DATCUN010000162         8135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_156958, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000162 DATCUN010000000
VERSION     DATCUN010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156958"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..699)
                     /gene="tpx"
                     /locus_tag="VIO83_06615"
     CDS             complement(202..699)
                     /gene="tpx"
                     /locus_tag="VIO83_06615"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEY8330980.1"
                     /translation="MSQVTLKGNPVRVDGQLPQVGAQAPAFRLVAADLSDKSLADFAG
                     QRKVLNIFPSIDTPTCATSVRKFNVEASKLSNTVVLCVSADLPFAQKRFCAAEGLDKV
                     VSLSTLRGREFLKDYGVAIAEGPLTGLAARAVVVLDENDKVLHSQLVAEIGNEPDYAA
                     ALAVL"
     gene            935..1747
                     /gene="xthA"
                     /locus_tag="VIO83_06620"
     CDS             935..1747
                     /gene="xthA"
                     /locus_tag="VIO83_06620"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY8330981.1"
                     /translation="MKLVSFNINGLRARPHQLAELIERHQPDVIGLQETKVADEQFPR
                     AAVEALGYHVEYHGQKGHYGVALLSRQAPQNLVKGFPWDGEESQRRFIGATFTDAHGQ
                     PLHVFNGYFPQGESRNHATKFPAKTQFYADLQRLLEEQFSPAQAVVVMGDFNISPQDS
                     DIGIGAENAKRWLRSGKCSFLPEEREWMARLHGWGLVDSFRALHPEVNDRFSWFDYRS
                     RGFEDQPKRGLRIDLIMTSSGLTPRLQAAGIDYELRGMDKPSDHAPVWLQLD"
     gene            1864..3231
                     /locus_tag="VIO83_06625"
     CDS             1864..3231
                     /locus_tag="VIO83_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HEY8330982.1"
                     /translation="MLRLPLQRSLRAHLLLGGLLATAIPTGSFAALPLPTDGQPALSI
                     QGSNTIGAKLGPALVGGLLEKEGFTDVRIVPGGENEQRVTARDDKGRMVNIQVAAHGS
                     STGFVALGNGSADLAASSRPIKDSEAASLVKFGDLKSPEGEQVIAIDGLAVILHPSNP
                     LKALSVPQLAKVFAGEVKTWEELGGRGGAINLYARDDNSGTWDTFKELVLTSQGKSLA
                     ATAKRFESSTQLSDSVSGDAQGIGFIGLPYIRQAKALAISAGDSQPMQPSAELVATED
                     YPLSRRLFLYVKPNETNPWAQQLVSFAHSPRGQEIVAKSGFISQTVKAMKVQLNGDMP
                     AGYRTLATDAQRLAVNFRFAEGSAELDNKALRDVRRVLRYLKDNNKMMSQVVLVGFGD
                     PKNDNPERALLLSKLRAVAVQRELVKSGVLVREVSGLGDALPVASNDSEEGREKNRRV
                     EVWVY"
     gene            3466..4446
                     /locus_tag="VIO83_06630"
     CDS             3466..4446
                     /locus_tag="VIO83_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY8330983.1"
                     /translation="MHALQAVLFADVLGTATWLWLTFFLLVFALLAFDLGVLHRDHRE
                     IGIGASLWLSAGYISVGLCFGAWVWYQMGATSALEYYTGFLIEKSLSMDNVFIMAMIF
                     GFFGIPRRYQHDVLFWGILGVIVLRALMIGLGAALIHHFEWVLHAFGVFLLATGVKML
                     FSPLDDSPGLLARNPLVLLLRRRMRVTEQLHEHHFFVQLPDAAGHLRRHATPLLLALV
                     LIELADLLFAVDSIPAIFAITQDPFIVYTSNIFAVLGLRALYFALAAMIHRFVYLKYA
                     LALVLVFIGAKIFVAGMFGKIPAPVSLAVTFTLLAGGVLFSLYKTRPRRD"
     gene            4540..4818
                     /locus_tag="VIO83_06635"
     CDS             4540..4818
                     /locus_tag="VIO83_06635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8330984.1"
                     /translation="MPIAMARHILVKTAAEAEQLKLRLEKGEDFATLARRHSFCPSRK
                     RGGDLGEIRPGQMVKPFENVVFRKPLHQVHGPLKTQFGWHLVLVYLRS"
     gene            complement(4906..6054)
                     /locus_tag="VIO83_06640"
     CDS             complement(4906..6054)
                     /locus_tag="VIO83_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY8330985.1"
                     /translation="MNITEMLRILASQDGSDLYLSTGAPPCAKFNGALRALSKEPLGP
                     GDVAKIAAEVMDAEQKVDFERELEMNLAISLPNIGRFRINIFKQRNEVSIVARNIKLD
                     IPRFEDLKLPPVLLDVILEKRGLVLFVGGTGSGKSTSLAALIDHRNRNHSGHIITIED
                     PVEYVHKHKKSIINQREVGVDTRSFQAALKNTLRQAPDVILIGEIRDRETMEHALAFS
                     ETGHLAISTLHANNANQALDRIINFFPEERRPQLLNDLGNNLKAFVSQRLVKTVDGKR
                     RAAVEILMGTATIRDLIKRGDFNLIKEVMEKSRPLGMQTFDQALIDLVNEGAISEEEA
                     VKNADSANNVRLKLKLHRDGEGGGQAKPDDTSTWSLAPTMEEEEEPTA"
     gene            6236..7825
                     /locus_tag="VIO83_06645"
     CDS             6236..7825
                     /locus_tag="VIO83_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004884468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEY8330986.1"
                     /translation="MISTANITMQFGAKPLFENVSVKFGNGNRYGLIGANGCGKSTFM
                     KILGGDLEPSSGQVMLDANTRLGKLRQDQFAYEEFNVIDTVIMGHEELWRVKAERDRI
                     YSLPEMSEEDGMAVAELETEFAEMDGYTAESRAGELLLGLGIPLAQHFGPMSEVAPGW
                     KLRVLLAQALFSNPDVLLLDEPTNHLDINTIRWLENILTARNSTMIIISHDRHFLNSV
                     CTHMADLDYGELRLFPGNYDEYMTAATQSREQLLADNAKKKAQIAELQTFVSRFSANA
                     SKAKQATSRAKQIDKIQLAEVKPSSRVSPFIRFEQTKKLHRQAVTVEHMSKGFDGKPL
                     FKNFSFSVEAGERVAIIGPNGIGKTTLLRTLVGDLTPDSGSVKWTESAEVGYYAQDHA
                     SDFEDDMTLFDWMGQWTQGGEQLVRGTLGRMLFSNDEILKSVKVISGGEQGRMLFGKL
                     ILNKPNVLVMDEPTNHLDMESIEALNLALENYPGTLIFVSHDREFVSSLATRIIELSE
                     DGVTDFSGTYDDYLRSQGVAA"
     gene            complement(7923..>8135)
                     /locus_tag="VIO83_06650"
     CDS             complement(7923..>8135)
                     /locus_tag="VIO83_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEY8330987.1"
                     /translation="PENTLHAVELLGGWPGALVAQQVFRHKTRKVSYQVVFWGIVALH
                     QAFWVDWLLLGGKFARQVLPQWVGLL"
BASE COUNT         1453 a         2761 c         2513 g         1408 t
ORIGIN      
        1 gtgcggtcgg cgcgggcctc ggcggcgctg ccggcggcgc catcagcaat ggcatgggcc
       61 gctgatcacg accacgccga gcccagcgtt cacaacaaaa agcccgcgaa agcgggcttt
      121 ttgttgcctg gtcgcctgac ctctacgctc aggccggcaa ccatgccagc ccaccgaggc
      181 aggcttcggg agcctggcga ctcagagcac cgcaagcgcc gccgcgtagt ccggctcgtt
      241 gccgatctcg gctaccagtt ggctgtgcag caccttgtcg ttctcgtcca gcaccaccac
      301 ggcgcgggcg gccaggccgg tcagcgggcc ttcggcgatg gccacgccgt aatccttgag
      361 gaactcgcga ccgcgcaggg tcgacaggct gaccaccttg tccaggcctt ccgcggcgca
      421 gaagcgcttc tgcgcgaacg gcaggtcggc cgagacgcac aacaccacgg tattgctcag
      481 cttactggcc tcgacgttga acttgcgcac cgaggtggcg caggtcgggg tatcgatgct
      541 ggggaagatg ttcaacacct tgcgctggcc ggcgaagtcg gcgagggact tgtcggacag
      601 gtcggcggct accaggcgga aggccggggc ctgggcaccg acctgcggca gctggccatc
      661 cacgcgcacc gggttgccct tcagggtcac ttggctcatt gctagaatcc tttattttaa
      721 attggttaag tacgaaacct agagcttcga aagagctatt ccgcgccccg tcagcgcaga
      781 aagcatcccg acagcggcgt gccgaaagcc tagcatgcgg ccgaacaggc aaatccatgc
      841 atttcgagct ggtactggcc agcgccgcac ggtggccggg acggggacga tcggctagga
      901 tcgccctcga actgtcaccc acggaaaacc gcccatgaaa ctcgtttcct tcaatatcaa
      961 tggcttgcgc gcacgtcctc atcagctggc tgagctgatc gagcggcacc agccggatgt
     1021 catcggtctg caggaaacca aggtggcgga cgagcagttt ccccgcgccg cggtcgaggc
     1081 gctcggctat cacgtcgagt atcacggcca gaagggccac tatggcgtcg ccctgctctc
     1141 gcgccaggcg ccgcagaacc tggtcaaggg cttcccgtgg gacggcgagg agtcgcagcg
     1201 gcgcttcatc ggcgccacct tcaccgacgc ccatggccag ccgctgcacg tgttcaacgg
     1261 ctacttcccg cagggcgaga gccgcaacca cgcaaccaag ttcccggcca agacgcagtt
     1321 ctacgccgac ctgcagcgcc tgctcgaaga gcagttcagc cccgcacagg ccgtggtggt
     1381 gatgggcgac ttcaatatct cgccgcagga cagcgacatc ggcatcggcg cggaaaacgc
     1441 caagcgctgg ctgcgcagcg gcaagtgcag cttcctgccg gaggagcgcg agtggatggc
     1501 ccgcctgcac ggctggggtc tggtggacag cttccgggca ctgcaccccg aggtgaacga
     1561 ccgcttcagc tggttcgact accgcagccg cggcttcgag gaccagccca agcgcggcct
     1621 gcgcatcgac ctgatcatga cctcgagcgg cctgacgcca cgcctgcaag ccgcgggcat
     1681 cgactacgag ctgcgcggca tggacaagcc gtcggatcac gcgccggtgt ggctgcaact
     1741 cgactgagtc gccattgcga cgctctgcca cgggcctgtc acgccagctg tcaccatgct
     1801 gtcattttac tgacttattc tgcgcggcat cctcaaacct gaccaacaag gttccctgcc
     1861 gccatgctgc gcctccccct tcagcgttcc ctccgtgccc acctgctgct cggcgggctg
     1921 ctggccaccg ccatccccac cggcagcttt gccgccctgc ccctgccaac cgatggccag
     1981 ccggcactga gcatccaggg ctccaacacc atcggcgcca agctgggccc ggcgctggtc
     2041 ggcggcctgc tggagaagga aggcttcacc gatgtgcgca tcgtgcccgg cggcgagaac
     2101 gagcagcggg tcaccgcgcg tgacgacaag ggccgcatgg tcaacatcca ggtggccgcg
     2161 cacggctcga gcaccggctt tgtcgccctg ggcaacggca gcgccgatct ggccgcttcc
     2221 tcgcgaccga tcaaggacag cgaagccgcc agcctcgtca agttcggtga cctgaagagt
     2281 ccggaaggcg agcaggttat cgccatcgac ggcctggcgg tgatcctgca cccatccaac
     2341 ccgcttaagg cgttgtcggt accgcagctg gccaaggtat tcgccggcga ggtgaagacc
     2401 tgggaggagc tgggcggccg tggcggcgcg atcaacctgt acgcccgcga cgacaactca
     2461 ggcacctggg acaccttcaa ggaactggtg ctgaccagcc agggcaagag cctggccgct
     2521 accgcgaagc gcttcgagtc gagcacccag ctgtccgact cggtcagcgg cgacgcacag
     2581 ggcatcggct tcatcggcct gccctacatc cgccaggcca aggcactggc gatcagtgcc
     2641 ggcgactcgc agccgatgca gccgagcgcc gaactggtgg ccaccgagga ttatccgctg
     2701 tcgcgtcgcc tgttccttta cgtcaagccg aacgaaacca atccctgggc ccagcaactg
     2761 gtcagcttcg cccacagccc gcgcggccag gaaatcgtcg ccaagagcgg cttcatctcg
     2821 cagaccgtga aggcgatgaa ggtccagctc aacggcgaca tgccggccgg ctaccgcacg
     2881 ctggcgaccg atgcccagcg tctggccgtc aacttccgct tcgccgaagg cagcgccgag
     2941 ctcgacaaca aggccctgcg cgacgtacgc cgggtactgc gctacctgaa ggacaacaac
     3001 aagatgatga gccaggtggt gctggtcggt ttcggcgacc cgaagaacga caaccccgag
     3061 cgcgccttgc tgctgtccaa gctgcgggcc gtcgccgtcc agcgcgaact ggtgaagagc
     3121 ggcgtgctgg tgcgcgaagt cagcggcctc ggcgatgccc tgccagtggc ctccaacgat
     3181 agcgaagaag gccgcgagaa gaaccgtcgc gtggaagtct gggtctactg atccggcgca
     3241 ggatcgggcc gcgggcggcg catgccgcac cgcagggccc gagtgcgacg cggctcgacc
     3301 cggtcacccc gccacccgcg catggccgga aaatcctgta accaaagccc gcttgcctga
     3361 ttgccgcgca cgacctagat tcttcaacga ccccgggccc ctctgacggt cgacagccgc
     3421 catgtcggag tcgctgacgc cgacaagcgg tgtccggagg gtgtcatgca cgcactgcaa
     3481 gctgtcctct tcgccgatgt tctcggcacc gccacctggc tgtggctgac cttcttcctg
     3541 ctcgtcttcg ccctgctggc cttcgacctc ggtgtcctgc accgcgacca tcgggaaatc
     3601 ggcatcggcg ccagcctctg gctgtccgcc ggctatatca gtgtcggcct ctgcttcggc
     3661 gcctgggtct ggtaccagat gggcgccacc agcgcgctgg agtactacac cggcttcctg
     3721 atcgagaaat cactgtcgat ggacaatgtg ttcatcatgg cgatgatctt cggcttcttc
     3781 ggcattcccc gccgttacca gcacgatgtg ctgttctggg gcattctcgg cgtgatcgtg
     3841 ctgcgggcgc tgatgatcgg cctgggcgcg gcgctgatcc accatttcga gtgggttctc
     3901 cacgccttcg gcgtgttcct gctggcaacc ggggtgaaaa tgctgttcag ccccctcgac
     3961 gacagccccg gcctgctggc cagaaacccg ctggtgctcc tgctgcgccg gcgcatgcgg
     4021 gtcaccgagc aactgcacga gcaccacttc ttcgtccagc tgccggatgc tgccggtcac
     4081 ctgcgacgcc atgccacccc gctgctgctc gccctggtgc tgatcgaact ggccgacctg
     4141 ctgttcgccg tggacagcat cccggcgatc ttcgccatca cccaggatcc cttcatcgtc
     4201 tacacctcga acatctttgc cgtgctcggc ctgcgcgccc tgtacttcgc cctggcggcg
     4261 atgatccacc gcttcgtcta tctgaagtac gccctggcgc tggtgctggt attcatcggc
     4321 gcgaagatct ttgtggccgg catgttcggc aagatcccgg caccggtgtc gctggccgtc
     4381 acctttaccc tgctggccgg tggcgtgctg ttttccctgt acaagacccg cccccgcagg
     4441 gactgagcga ggcgcagatc cactgcccag tggcgcaggc aaactggcgg tcttcggtta
     4501 cactgcgcgc ccgctgccat tcacccgagg accccgccga tgcccatcgc catggcccgc
     4561 cacatcctgg tcaagaccgc cgccgaagcc gagcagctca agctgcgcct ggagaaaggc
     4621 gaggacttcg cgaccttggc ccggcgccat tcgttctgcc cctcgcgcaa gcgcggcggc
     4681 gatctgggcg agatccgccc ggggcagatg gtcaagccgt tcgagaacgt ggtattccgc
     4741 aagccgctgc atcaggtcca cggcccgctg aagacccagt tcggctggca tctggtgctg
     4801 gtctacctgc gcagctgaaa cgccagtgcc ggagccacct gggtggactc cggcactggt
     4861 gcgcccgcag gcaccgcgcc gttcaggcgg cggcgccgag gaagatcaag ccgtcggctc
     4921 ttcttcctct tccatggtcg gcgccaggct ccaggtcgag gtatcgtcag gcttggcctg
     4981 accaccgccc tcgccgtcgc ggtgcagctt gagcttgagg cgcacgttgt tcgccgagtc
     5041 ggcgttcttc accgcttcct cttcgctgat cgcgccctcg ttgaccagat cgatcagcgc
     5101 ctggtcgaag gtctgcatgc ccagcggccg cgacttctcc atgacctcct tgatcaggtt
     5161 gaaatcgccc cgcttgatca ggtcgcggat ggtcgcggtg cccatcagga tctccaccgc
     5221 ggcgcgccgc ttgccgtcga cggtcttgac cagacgctgg gagacgaacg ccttgaggtt
     5281 gttgccgagg tcgttgagca gctgcggccg acgctcctcg gggaagaagt tgatgatgcg
     5341 gtccagcgcc tggttggcgt tgttggcgtg cagggtggag atcgccaggt ggccggtctc
     5401 ggagaaggcc agggcgtgtt ccatggtctc gcggtcgcgg atctcgccga tcaggatgac
     5461 atccggcgcc tggcgcaggg tgttcttcag cgccgcctgg aagctgcggg tatccacgcc
     5521 cacctcgcgc tggttgatga tcgacttctt gtgcttgtgc acgtactcca ccgggtcctc
     5581 gatggtgatg atgtgaccac tgtggttgcg gttgcggtgg tcgatcagcg ccgccagcga
     5641 ggtcgacttg cccgagccgg taccaccgac gaacagcacc aggccgcgct tctcgaggat
     5701 cacgtcgagc agcaccggtg gcagcttgag atcctcgaaa cgcgggatat ccagcttgat
     5761 gttgcgcgcg acgatggaca cctcgttgcg ctgcttgaag atgttgatcc ggaaacgacc
     5821 gatattgggc agggagatgg ccaggttcat ctccagctcg cgctcgaagt cgaccttctg
     5881 ctcggcatcc atcacctcgg cggcgatctt cgccacgtcg cccggcccca gcggctcctt
     5941 gctcagcgcg cgcagcgcac cattgaactt ggcgcagggc ggcgcaccgg tcgaaagata
     6001 caggtcggat ccatcctggc tggccaggat gcgcagcatt tccgtgatgt tcatcgagat
     6061 aaacgccgta acaggttgat ctaaaaggcc accattctgc atcaagcaat gcgtgacgcc
     6121 catcacggtt atccggattg agaagccgtc gatcctttag gctgccgcga ctgacgcaaa
     6181 acgctacaat gtacgttttt tgcgctcccg cgccctctcc ttgcaggtca gattcgtgat
     6241 ttccaccgct aacatcacca tgcagttcgg cgccaagccg ctgttcgaga acgtttccgt
     6301 caagttcggc aacggcaacc gctacggcct gatcggcgcc aacggttgcg gcaagtcgac
     6361 gttcatgaag atcctcggcg gcgatctgga accttccagc ggccaggtga tgctcgatgc
     6421 caacacccgc ctgggcaagc tgcgccagga ccagttcgcc tacgaagaat tcaacgtgat
     6481 cgacaccgtg atcatgggcc acgaggagct gtggcgggtc aaggccgagc gcgatcgcat
     6541 ctactcgctg ccggaaatga gcgaggaaga cggcatggcc gtggccgagc tggaaaccga
     6601 gttcgccgag atggacggct acaccgccga atcccgcgcc ggtgagctgc tgctcggcct
     6661 gggcattccg ctggcccagc acttcggccc gatgagcgaa gtggcaccgg gctggaaact
     6721 gcgcgtgctg ctggcccagg cgctgttctc caacccggac gtgctgctgc tcgacgagcc
     6781 gaccaaccac ctggacatca acaccatccg ctggctggaa aacatcctca cggcgcgtaa
     6841 cagcaccatg atcatcattt cccacgaccg ccacttcctc aacagcgtgt gcacgcacat
     6901 ggcggacctg gactatggcg agctgcgcct gttcccgggc aactatgacg agtacatgac
     6961 cgccgcgacc cagtcgcgcg agcagctgct ggccgacaac gccaagaaga aggcgcagat
     7021 cgccgaactg cagaccttcg tcagccgctt ctcggccaac gcctccaagg ccaagcaggc
     7081 cacctcgcgc gccaagcaga tcgacaagat ccagctggcc gaggtcaagc cgtccagccg
     7141 cgtcagcccg ttcatccgct tcgagcagac caagaagctg caccgccagg cggtgactgt
     7201 cgaacacatg tccaagggct tcgatggcaa gccgctgttc aagaacttca gcttcagcgt
     7261 cgaagcgggc gagcgcgtag ccatcatcgg ccccaacggc atcggcaaga ccaccctgct
     7321 gcgcaccctg gtcggcgacc tgactccgga cagcggctcg gtgaagtgga cggaaagcgc
     7381 cgaggtcggt tactacgccc aggatcacgc ctccgatttc gaggacgaca tgaccctgtt
     7441 cgactggatg ggccagtgga cccagggcgg cgagcagctg gtgcgcggca ccctcggccg
     7501 catgctgttc agcaacgacg agatcctcaa gtcggtcaag gtcatctccg gtggcgaaca
     7561 gggccgcatg ctgttcggca agctgatcct caacaagccc aacgtgctgg tgatggacga
     7621 accgaccaac cacctcgaca tggagtcgat cgaggcgctc aacctggcgc tggagaacta
     7681 cccgggcacg ctgatcttcg tcagccacga ccgcgagttc gtcagctccc tggccacccg
     7741 catcatcgag ctgtcggaag acggcgtgac cgacttcagc ggcacctacg acgactacct
     7801 gcgcagccag ggcgtcgcgg cctgaggtat ccagccggcc cgatgggccg gctgattttc
     7861 tgtaggcgcg aattcattca cgatagtggc tggccagcaa ccggtcgcgg ataaatccgc
     7921 gcctacagga gccctaccca ctgcggcagt acctgccgag cgaacttccc gcccaacagc
     7981 agccagtcga cccagaacgc ctggtgcagc gcgacaatcc cccagaacac cacctgatac
     8041 gagaccttgc gggtcttgtg cctgaacacc tgctgcgcca ccagcgcacc cggccagccg
     8101 ccgagcagct cgacggcgtg cagggtgttt tccgg
//
LOCUS       DATCUN010000163        14715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_231954, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000163 DATCUN010000000
VERSION     DATCUN010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 14715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14715
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231954"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4034
                     /locus_tag="VIO83_06655"
     CDS             <1..4034
                     /locus_tag="VIO83_06655"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEY8330988.1"
                     /translation="RQLSATALAWLRTPCRLAFAKADQPARVHRAVYPDYVSLRELDG
                     DGRVLRECRFMGLYTSTVYGESVQRIPCVRGKVAEVLRRSGFAPGSHLGKGLLQTLEG
                     LPRDDLFQTPVAELTATAVAIVQIQERNRLRLFLRHDPFGHFCYGLAYVPREIYSSEI
                     RRKIQQLLVQRLGASDCEYWTEFSESVLARVQFILRVDPRQPPQVEVRQLEQEVIQAC
                     RSWQDDYAGLLLESFGEAQGSDLLASFAGGFPAGYRERFEPHQALTDLQHLLGLNAER
                     PLRMSFQVPAQAGAELHGRIYHAATPLPLSDLLPVLENLGLRVLGEFSFCLRRQDGRE
                     YWIHDLSFSPLAGADPAALGELLEDAFVHIVQGAAENDGFNRLVLGAGLAWREVALLR
                     AYGRYLKQIRLGFDLGYIAATLGNHAAIAGELVRLFKVRFHLAQRLPVAELADKQQRL
                     EQAILVALEQVQVLNEDRILRRYLELIRATLRSNYYRTGADGRHRDYLSLKLDPQQIA
                     EMPRPVPRYEIFVYSPRVEGVHLRGGKVARGGLRWSDREEDYRTEVLGLVKAQQVKNT
                     VIVPVGAKGGFVPRHLPPGGSREAIQAEAIACYRIFVSGLLDLTDNLVDGLVVPPAQV
                     VRYDDDDPYLVVAADKGTASFSDIANALAADYGYWLGDAFASGGSAGYDHKGLAITAR
                     GAWVAVQRHFRELGIDVQQDRISVVGIGDMAGDVFGNGLLRSDRLQLVGAFNHQHIFL
                     DPDPDPALSFAERQRLFALPRSTWADYDAALISPGGGVFPRSAKRIAISPQMRARLAI
                     AAEQLTPAELIGALLRAPVDLLWNGGIGTYVKSSREGHLEVGDKANDAVRVDGRELRC
                     QVVGEGGNLGMTPLGRVEYCLYGGACNTDFIDNAGGVTCSDQEVNIKILLGEVVRAGD
                     LTLKQRDALLHEMSEAVATQVLAVVYRQTQALSLAQRYARGRLGEFRRLMVDLESRGR
                     LDRALEFLPGDDELNARAAQGQALTRPELALLLAYSKFDLKEALLHSPLVDEPYLRGE
                     LQRAFPLPLVQRFADALEHHRLRREIISTRLANDLIDHMGLTFVQRLHESTGLTAAHI
                     AAGWVVVRDLFDLPRWWQQLEALDQCIDAELQLALMEELMRLGRRATRWLLRSRRHEL
                     DMAVDVEHFAPGVRELAGSLGELLEGPPREAWQARRAELEGAGVPAELAQVLAAGDQL
                     YTLLAIVDAADATGQPPLRVAACHFAVGAELELGWYLQQLNALPVESNWQALARESLR
                     DELDSQQRAITVAVLQMDDGPLEIDNRLTLWLAHHQPLVARWRQLLVELRAVGAGDYT
                     VYTVANRELQDLAQSAQRGECIP"
     gene            4081..5421
                     /locus_tag="VIO83_06660"
     CDS             4081..5421
                     /locus_tag="VIO83_06660"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY8330989.1"
                     /translation="MKSPEELLHFAERLMAVEEIDCVTPDLNGIPRGKTMTVAGFLSG
                     RRLQMARGVLLQCLMGGYPPTRFYGSDDSDLALRADPQQVHILPWSEPPRALAICDAL
                     EIDGSPCALSTRGLLRRVLAEYAALGLTPVVATELEFYVFAAHPDPREPFQPPLGLDG
                     RREIGASAFSVASSHGLQPFFAEVYRGMAALGLPRDTFMHEMGTSQFEINFPHGDALQ
                     LADQTFLFKHLLREVALRHGLLAVCMAKPLGGVPGSSMHIHQSLVDEAGRNVFSAADG
                     EPTAAFAQFIGGLQAALAELTLLMAPNVNSYHRLAQPYASPNNACWAYDNRAAGLRIP
                     ASAAQDRRVENRLPGADANPYLALAASLGAGLFGLVEGLQPTLPVAGAFTVPEALRLP
                     TSMNEALHGLQRSVLARELFGEAFVDGYLASKTLELDSFLEEISPWERRLLAQV"
     gene            5649..7118
                     /locus_tag="VIO83_06665"
     CDS             5649..7118
                     /locus_tag="VIO83_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8330990.1"
                     /translation="MWRESLILLIDDNQQRRHDLAVILDFLGEPCSSCTSADWAERLA
                     GEDVARLSCVLLGRIDGKGGAPRLLKQLGPQAANLPLLLVEEQGSGDWPDDLRRRVLA
                     RLDMPPSYNQLLDSLHRAQVYLQVFDVARERGLQRETNLFRSLVGTSRAILQVRQQVQ
                     QVAGTEVGVLLLGESGTGKEVVARNLHYHSSRREFPFVPISCTAIAPELLESELFGYE
                     KGGFAGAISSHKGRLELAAGGTLYIDEVADLPAELQGRLLRVLQEGRFKRFGSDEEQL
                     IDVRIIAASRHDLEARVAAGTFRDDLYYRLNQFSIELPPLRERVEDIPLLLNELIARM
                     EHEKRGSIRFSSAALMSLCRHDWRGNVRELANLVERLAIMHPYGVMGVAELPKKFRHL
                     DGESAQEVDGVRRELDSPVMPVGALPGLDAPALLPVGGLDLKDYLGNLERSLIQQALD
                     DAGGVVARAAERLRLRRTTLVEKMRKYGMSRRDDEEGEL"
     gene            7163..7987
                     /locus_tag="VIO83_06670"
     CDS             7163..7987
                     /locus_tag="VIO83_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEY8330991.1"
                     /translation="MPGPVQVIAIASGKGGVGKTQTAINLACALAARGRRVLLLDASF
                     ALPNIDVALGLTPSCTLHDVLAGRCNLADALVSGPGGVQVIAGASAAGALLEPSVQQL
                     GGLIRAFDELPQAPDVLLVDCAPGAGSAVLSLLQAASEVLLVVNDEPAALSDALALMR
                     RLNQRHGMTRFRLLASMTFAQQAGEALHERLLRLSEPLAGLSLDYVGAIPFDDSLRRA
                     VQRQRPLLEVFPRSRASQAYAAVAEKVDGWPLPANPRGHLEFFVERLIAAQAIPRG"
     gene            8133..8816
                     /gene="ccmA"
                     /locus_tag="VIO83_06675"
     CDS             8133..8816
                     /gene="ccmA"
                     /locus_tag="VIO83_06675"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEY8330992.1"
                     /translation="MLSIAPPPGSHAVTSPFLEAVALACERDLRVLFERLDFALHAGE
                     MLQIAGPNGSGKTSLLRLLAGLMQPTAGEIRLAGRPLAEQRGELTRNLLWVGHAAGIK
                     GLLSPEENLAWLCALHTAETREAIWRALQAVGLRGFEDVPCHTLSAGQQRRVALARLY
                     LDPPPLWVLDEPFTALDKHGVAQLEEHLARHCEQGGMVVLTTHHTLTRKPAGYRELDL
                     AQAGSTADE"
     gene            8809..9480
                     /gene="ccmB"
                     /locus_tag="VIO83_06680"
     CDS             8809..9480
                     /gene="ccmB"
                     /locus_tag="VIO83_06680"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY8330993.1"
                     /translation="MSNVFTQLLTREARLLFRRPADLLNPLVFFGIVVALFPLAVGPE
                     SKLLETLSPGLVWVAALLAVLLSLDGLFRSDFEDGSLEQWVLSPHPLPLLVLAKVLAH
                     WLFSGLALVLLAPLLALMLGLPARCVPVLLLSLLLGTPILSLLGAVGAALTVGLKRGG
                     LLLALLILPLYIPVLILGSGALQSALQGLPVAGHLLWLASLTALAVTLTPFAIAAGLT
                     ISVGE"
     gene            9516..10259
                     /locus_tag="VIO83_06685"
     CDS             9516..10259
                     /locus_tag="VIO83_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HEY8330994.1"
                     /translation="MNWTWFHKLGSPKWFYDISGRWLPWLGAAAALLIAVGTVWGLAF
                     APPDYQQGNSFRIIYIHVPAAILAQSCYMMLAVAGVVGLVWKMKLADVALQCAAPIGA
                     WMTFIALVTGAVWGKPTWGAWWVWDARLTAMLILLFLYFGVIALGQSISNRDSAAKAC
                     AVLAIVGVINIPIIKYSVEWWNTLHQPATFTITEKPAMPAEMWVPLLIMVIGFYCFFA
                     VALLLRMRLEVLKRESRASWVKAEVERSL"
     gene            10256..10432
                     /gene="ccmD"
                     /locus_tag="VIO83_06690"
     CDS             10256..10432
                     /gene="ccmD"
                     /locus_tag="VIO83_06690"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEY8330995.1"
                     /translation="MSFESFADFLAMGKHGLYVWTSYGISLAVLALNVALPILARRRY
                     LHDEARRLRRESKQ"
     gene            10429..10911
                     /gene="ccmE"
                     /locus_tag="VIO83_06695"
     CDS             10429..10911
                     /gene="ccmE"
                     /locus_tag="VIO83_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY8330996.1"
                     /translation="MNPVRKKRLFIILGILAGVGVAVGLALSALQQNINLFYTPTQIA
                     AGEAPQDTRIRAGGMVREGSVSRSADSLDVQFVVTDFAKDVTIQYRGILPDLFREGQG
                     IVALGRLNEEGVLVADEVLAKHDEKYMPPEVTKALKDSGKLPEGSMPASAGAKMGGAQ
                     "
     gene            10908..12881
                     /locus_tag="VIO83_06700"
     CDS             10908..12881
                     /locus_tag="VIO83_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY8330997.1"
                     /translation="MIPELGHLAMILALCLAVVQASLPLIGAWRGDRQWMGLARPAAW
                     GQFAFLLFSFLCLTQAFLVDDFSVAYVAKNSNSALPWFYKFSAVWGAHEGSLLLWALI
                     LGAWTFAVSIFSRQLPEVMLARVLSVMGMISVGFLLFLIVTSNPFERLLPNAPTDGRD
                     LNPLLQDIGLIAHPPMLYMGYVGFSVVFAFAIAALLGGRLDAAWARWSRPWTIVAWAF
                     LGIGIVLGSWWAYYELGWGGWWFWDPVENASLMPWLVGTALLHSLAVTEKRGVFKSWT
                     VLLAIAAFSLSLLGTFLVRSGVLTSVHAFAADPERGTFILIFLLFVVGSSLTLFALRA
                     PVVKSNIGFGLWSRETLLLANNLVLVVACAMVLLGTLYPLVLDALTGAKLSVGPPYFN
                     ALFLPLMAILMLALGVGVLVRWKDTPLKWLSGMLLPVLVVSGAFGLLGAWLFGDFHWA
                     VLATCLLAAWVVLAGLRDLLDKTRHKGLLKGIAGLSRSYWGMQIAHLGIAVTALGVVL
                     SSQYALERDLRMAPGESVEVGGYQFVFDGAQHHQGPNYTSDRATVHVLQDGREITVLH
                     PEKRVYPVRGMPMTEAGIDPGFTRDLYVALGEPLEDGAWAVRLHVKPFVRWIWLGGLL
                     MGFGGFLAALDRRYRVKVTTKAREALGMTGAQA"
     gene            12878..13411
                     /locus_tag="VIO83_06705"
     CDS             12878..13411
                     /locus_tag="VIO83_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEY8330998.1"
                     /translation="MKRAILLLPLALFLGLAVFLYRGLFLDPAELPSALIGKPFPAFA
                     LPSVHDSARTLSAADLKGRPALVNVWATWCPTCKAEHQMLNQLAGQGVNIIGVNYKDD
                     NAAARKWLKDFLDPYQLNIEDKVGSLGLNLGVYGAPETFLIDKDGIIRHKYVGAIDER
                     VWREQLAPLYQELVDAR"
     gene            13411..13947
                     /locus_tag="VIO83_06710"
     CDS             13411..13947
                     /locus_tag="VIO83_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEY8330999.1"
                     /translation="MKRLLSKRWLAGGLLGLTLSLGAQAAIDTYEFKDEGDRARYRLL
                     TEELRCPKCQNQNIADSDAPIAMDLRREIHRMLGEGKSNDEIVDFLVARYGDFVRYNP
                     PVNARTWLLWYGPWLLLGGGLGVLALIVLRRRRPAASGQGADLSAEERARLDQLLKNS
                     VHKSSAEQSSADNPKADK"
     gene            13944..>14715
                     /gene="ccmI"
                     /locus_tag="VIO83_06715"
     CDS             13944..>14715
                     /gene="ccmI"
                     /locus_tag="VIO83_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014597049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEY8331000.1"
                     /translation="MTAFWLAAAALLLVAFAFLLIPLLRGRRAQAEEDRTALNVALYQ
                     ERVAELDAQHAAGALDAAQRDAGRREAARELLDDAEQAEGAQRKLGRWLPLAMALLMP
                     VLGLGLYLHWGASDALKLSEQLATEPQNIEEMTARLEETVRLQPDQAEAWYFLGRTYM
                     AQQRPKEAAPAFGRAVELAGRQPELLGQWAQALYFAGGQKFTDEVRSLGEETLRLDPQ
                     EPTTLGLLGIAAFEEQRFVDAVRYWERLVATLQPNDPSR"
BASE COUNT         2070 a         5140 c         5093 g         2412 t
ORIGIN      
        1 agcgccagct gtcggccacg gccctggcct ggctgcgcac gccgtgccgg ctggccttcg
       61 ccaaggccga ccagccggcg cgggtgcacc gcgcggtgta tccggactac gtgtccctgc
      121 gcgagctgga cggggacggg cgggtgctgc gcgagtgccg cttcatgggc ctgtatacct
      181 ctacggtcta cggcgagagc gtgcagcgca ttccctgcgt gcgcggcaag gtggccgagg
      241 tgctgcgccg ttccggcttt gcgcccggca gccatctggg caaggggctg ctgcagacgc
      301 tcgaggggct gccgcgcgac gacctgttcc agactccggt ggctgaactc accgccaccg
      361 cggtggccat cgtgcagatc caggagcgca accgcctgcg cctgttcctg cgtcacgatc
      421 cgttcggcca cttctgctac ggcctggcct acgtgccgcg ggagatctat tcctcggaga
      481 tccgccgcaa gatccagcag ctgctggtgc agcgcctggg cgccagcgac tgcgagtact
      541 ggaccgagtt ctccgagtcg gtgctggcgc gggtgcagtt catcctgcgc gtcgatccgc
      601 gccagccgcc gcaggtcgag gtgcgccagc tcgaacagga agtgatccag gcctgccgca
      661 gctggcagga cgactacgcc ggcctgctgc tggaaagctt cggcgaggcc cagggcagcg
      721 atctgctggc cagcttcgcc ggcggctttc cggccggtta tcgcgagcgt ttcgagccgc
      781 accaggcgct gaccgacctg cagcacctgc tcggcctgaa tgccgagcgc ccgctgcgca
      841 tgagtttcca ggtgccggcg caggccggcg ccgagctgca cggcaggatc taccatgccg
      901 ccacgccgct gccgctgtcg gacctgctgc cggtgctgga gaacctcggc ctgcgcgtgc
      961 tcggcgagtt ctccttctgt ctgcgccgcc aggacggccg cgagtactgg atccacgacc
     1021 tcagcttcag cccgctcgcc ggcgccgacc cggcggcgct gggcgaactg ctcgaggatg
     1081 ccttcgtcca catcgtccag ggcgccgcgg aaaacgacgg cttcaaccgc ctggtgctcg
     1141 gcgccggcct ggcctggcgc gaggtggcgc tgctgcgcgc ctacggccgc tacctcaagc
     1201 agatccgcct gggcttcgat ctgggctaca tcgccgccac cctgggcaac catgccgcca
     1261 tcgccggcga actggtgcgc ctgttcaagg tgcgcttcca tctggcccag cgcctgccgg
     1321 tggccgagct ggccgacaag cagcagcgtc tggagcaggc catcctcgtc gccctggagc
     1381 aggtgcaggt gctcaacgag gaccgcatcc tgcgccgcta cctggagctg atccgtgcca
     1441 ccctgcgcag caactactac cgcaccggcg ccgacggccg gcaccgggac tacctgagcc
     1501 tcaagctcga tccgcagcag atcgccgaga tgccgcggcc ggtgccgcgc tacgagatct
     1561 tcgtctactc gccgcgggtc gagggcgtgc acctgcgcgg cggcaaggtg gcgcgcggcg
     1621 gcctgcgctg gtcggaccgc gaggaggact accgcaccga ggtgctcggc ctggtcaagg
     1681 cgcagcaggt gaagaacacg gtgatcgtgc cggtcggcgc caagggcggc ttcgtgccgc
     1741 gccacctgcc gcccggcggc tcgcgcgagg cgatccaggc cgaggccatc gcctgctacc
     1801 ggatcttcgt cagcgggctg ctcgatctca ccgacaacct cgtcgatggc ctggtggtgc
     1861 cgcccgcgca ggtggtgcgc tacgacgacg acgaccccta cctggtggtg gcggcggaca
     1921 agggcaccgc cagtttctcc gacatcgcca acgccctggc cgccgactac ggctactggc
     1981 tgggcgacgc cttcgcttcc ggcggttcgg ccggctacga ccacaagggc ctggcgatca
     2041 ccgcgcgcgg cgcctgggtc gcggtgcagc ggcatttccg cgagctcggc atcgacgtgc
     2101 agcaggaccg catcagcgtg gtcggcatcg gcgacatggc gggcgacgtg ttcggcaacg
     2161 gcctgttgcg ctcggatcgc ctgcaactgg tcggcgcctt caaccaccag cacatcttcc
     2221 tcgatcccga ccccgatccg gcgctcagct tcgccgagcg ccagcgcctg ttcgccctgc
     2281 cgcgctcgac ctgggccgac tatgacgccg cgctgatctc gccgggcggc ggggtgttcc
     2341 cgcgcagcgc caagcgcatt gccatcagcc cgcagatgcg cgcgcggctg gccatcgccg
     2401 ccgagcagct gaccccggcc gagctgatcg gcgcgctgct gcgcgcgccg gtcgacctgc
     2461 tgtggaacgg cggcatcggc acctacgtca agtccagccg cgagggccat ctggaggtcg
     2521 gcgacaaggc caacgacgcc gtgcgggtgg acggccgcga gctgcgctgc caggtggtgg
     2581 gcgagggcgg caacctcggc atgacgccgc tcggccgggt cgagtactgc ctgtacggcg
     2641 gtgcctgcaa caccgatttc atcgacaacg ccggcggggt gacctgctcg gaccaggagg
     2701 tcaacatcaa gatcctcctc ggcgaggtcg tgcgcgccgg cgacctcacc ctcaagcagc
     2761 gcgatgcgct gctgcacgag atgagcgagg cggtggcgac ccaggtgctg gccgtggtct
     2821 accggcagac ccaggcgctg tcgctggcgc aacgctatgc ccgcggccgg ctcggcgagt
     2881 tccgccggct gatggtcgac ctggagagcc gcggccgcct ggaccgcgcg ctggagttcc
     2941 tccccggcga cgacgagctg aatgcccgcg cggcccaggg ccaggcgctg acccgcccgg
     3001 agctggcgct gctgctggcc tacagcaagt tcgatctgaa ggaggcgctg ctgcactcgc
     3061 cactggtgga cgaaccctac ctgcgcggcg agctgcagcg cgccttcccg ctgccgctgg
     3121 tgcagcgctt tgccgatgcg ctggagcacc atcgcctgcg ccgcgagatc atcagcacgc
     3181 ggctggccaa cgacctgatc gaccacatgg ggctgacctt cgtccagcgc ctgcacgagt
     3241 ccaccggcct gaccgcggcg cacatcgccg ccggctgggt ggtggtgcgc gacctgttcg
     3301 acctgccgcg ctggtggcag cagctggagg cgctcgacca gtgcatcgac gccgagctgc
     3361 agctggcgct gatggaggag ctgatgcgcc tgggacggcg cgccacccgc tggctgctgc
     3421 gcagccgccg ccatgagctg gacatggccg tcgacgtcga acacttcgcc cccggggtgc
     3481 gcgagctggc cgggagtctc ggcgaactgc tcgaggggcc gccccgcgag gcctggcagg
     3541 cgcgtcgtgc ggagctggag ggtgccgggg tgccggccga actggcgcag gtgctggccg
     3601 ctggcgacca gctgtacacc ctgctggcga tcgtcgatgc cgccgacgcc accgggcagc
     3661 cgccgctgcg ggtggcggcc tgccacttcg ccgtcggcgc cgagctggag ctgggctggt
     3721 acctgcagca gctcaacgcc ctgccggtgg agagcaactg gcaggcgctg gcccgcgagt
     3781 cgctgcgcga cgagctggac agccagcagc gggccatcac cgtggcggtg ctgcagatgg
     3841 acgacggtcc gctggaaatc gacaaccgcc tgaccctgtg gctggcgcat caccagccgc
     3901 tggtggcgcg ctggcggcag ctgctggtgg agctgcgcgc cgtcggtgcc ggcgactaca
     3961 ccgtgtacac ggtggccaac cgcgagttgc aggacctggc gcagagtgcg cagcgcgggg
     4021 agtgcatccc ctgagggccg ataccttgca atcatccgca atgaagccga gaacgaaacc
     4081 atgaaaagcc cggaagaact gctgcacttc gccgagcgcc tgatggcggt cgaggagatc
     4141 gactgcgtga cgccggacct caacggcatc ccgcgcggca agaccatgac cgtcgcgggc
     4201 ttcctctccg gccggcgcct gcagatggcg cgtggcgtgc tgctgcagtg cctgatgggc
     4261 ggctacccgc cgacccgctt ctacggcagc gacgacagcg acctggcgct gcgcgccgat
     4321 ccgcagcagg tccacatcct gccgtggagc gagccgccac gggcgctggc gatctgcgat
     4381 gcgctggaaa tcgacggcag cccctgcgcg ctgtccaccc gcggcctgct gcgccgggtg
     4441 ctggccgagt atgcggcgct gggcctgacc ccggtggtgg ccaccgagct ggagttctac
     4501 gtcttcgcag cccaccccga tccgcgcgag ccgttccagc cgccgctggg cctggatggc
     4561 cggcgggaaa tcggcgcctc ggccttcagc gtggcttcca gccacggcct gcagccgttt
     4621 ttcgccgagg tctaccgggg catggcggcg ctgggcttgc cgcgcgacac cttcatgcac
     4681 gagatgggca ccagccagtt cgagatcaac ttcccccatg gcgacgccct gcagctggcc
     4741 gaccagacct tcctgttcaa gcacctgctg cgcgaggtgg cgctgcgtca cggcctgctg
     4801 gcggtgtgca tggccaagcc gctgggcggc gtgccgggca gctccatgca tatccaccag
     4861 agcctggtgg acgaggcggg gcgcaacgtg ttcagcgccg ccgacggcga accgacggcg
     4921 gccttcgccc agttcatcgg cggcctgcag gccgcgctgg ccgagctgac cctgctgatg
     4981 gcgcccaacg tcaattccta ccaccgcctg gcccagccct atgcctcgcc gaacaacgcc
     5041 tgctgggcct acgacaatcg tgccgccggc ctgcgcatcc cggccagcgc cgcgcaggac
     5101 cggcgggtgg agaaccgcct gccgggcgcc gacgccaatc cctacctggc cctggccgcc
     5161 agcctggggg ccgggctgtt tgggctggtg gaaggcctgc agccgacgct gccggtcgcc
     5221 ggcgccttca cggtgccgga ggccctgcgc ctgccgacca gcatgaacga ggcgctgcac
     5281 ggcctgcagc gcagcgtgct ggcgcgcgag ttgttcggcg aggcgttcgt cgacggctat
     5341 ctggcgagca agacgctgga actggacagt tttctcgagg aaatcagccc ctgggaacgg
     5401 cgcctgctgg cgcaggtctg acgacgagaa aatgacattg tgttcgccct gagtcgtcgc
     5461 ctcgcctcca atgaaaagcg ggcgatggct caaggttgct gcagggcaga tgctcggaaa
     5521 gtcacgtcat aaatttgact gttctcgttc ttttggcttt actgctggca ctttagcggc
     5581 atggctgtct ccgccagccg ccgaccttgg ctctgtgccc tttcctgccc cctcccgacc
     5641 gaccgatcat gtggcgtgaa tctctaatcc tgctgataga tgacaaccaa caacgccgcc
     5701 atgacctggc ggtgatcctc gacttcctcg gcgagccctg cagcagctgc accagcgccg
     5761 actgggccga acggctggcc ggcgaggatg tcgcccgcct gtcctgcgtc ctgctcggcc
     5821 ggatcgacgg caagggcggc gccccgcgcc tgctcaagca gctgggtccg caggcggcca
     5881 acctgccgtt gctgctggtc gaggagcagg gcagcggcga ctggccggac gacctgcgcc
     5941 gccgcgtgct ggcgcgtctg gacatgccgc cgagctacaa ccagctgctc gactcgctgc
     6001 accgcgcgca ggtctacctg caggtgttcg atgtcgctcg cgagcgcggc ctgcagcgcg
     6061 agaccaacct gttccgcagc ctggtcggca ccagccgggc gatccttcag gtgcgccagc
     6121 aggtgcagca ggtggccgga accgaggtcg gcgtgctgtt gctcggcgag tccggcaccg
     6181 gcaaggaagt ggtggcgcgc aacctgcact accactccag ccgtcgtgaa ttcccgttcg
     6241 taccgatcag ctgcacggcg atcgccccgg agctgctgga aagcgagctg ttcggctacg
     6301 agaagggggg cttcgccggt gccatcagca gtcacaaggg gcgcctcgaa ctggcggccg
     6361 gcggcaccct gtacatcgac gaggtcgcgg acctgccggc ggagctgcag gggcgcctgc
     6421 tgcgggtgct gcaggagggc cggttcaagc gcttcggcag cgacgaggag cagctcatcg
     6481 acgtgcgcat catcgccgcc agccgccatg acctggaggc gcgcgtggcg gccggtactt
     6541 tccgcgacga cctgtactac cggctcaacc agttttccat cgagctgccg ccgctgcgcg
     6601 agcgggtcga ggatattccg ctgctgctca acgagctgat cgcgcggatg gagcacgaga
     6661 agcgcggctc gatccgcttc agctcggcgg cgctgatgtc gctgtgccgg cacgactggc
     6721 gcggcaacgt gcgcgagctg gccaatctgg tcgagcggct ggccatcatg catccctatg
     6781 gggtgatggg cgttgccgag ctgccgaaga agttccgcca tctcgacggc gaatcggcgc
     6841 aggaggtcga cggcgtgcgc cgcgagctcg actcgccggt gatgccggtg ggcgccttgc
     6901 cggggctgga tgcgccggcg ctgctgccgg tcggtggcct ggatctcaag gattacctgg
     6961 gcaatctcga gcgctcgctg atccagcagg cgctggacga tgccggcggg gtagtggcgc
     7021 gcgccgccga gcggctgcgc ctgcggcgta ccacgctggt ggagaagatg cgcaagtacg
     7081 gcatgagccg gcgcgacgac gaggagggtg aactctagtg ggccggcagt tttcgggaag
     7141 gcgcgcggcg gccgcgaaga acatgccggg tccggtccag gtgatcgcca tcgccagcgg
     7201 caagggcggc gtcggcaaga cgcagacggc gatcaacctg gcctgcgcgc tggccgcgcg
     7261 gggtcggcgg gtattgctgc tggacgccag cttcgccctg cccaatatcg atgtggccct
     7321 cggcctgacg ccgtcgtgca ccctgcacga cgtgctggcc gggcgctgca acctggccga
     7381 tgcgctggtg agcgggccgg gcggagtgca ggtgatcgcc ggcgcctcgg cggcgggcgc
     7441 cctgctggag ccgagcgtgc agcagctggg cggactgatc cgggccttcg acgagctgcc
     7501 gcaggcgccg gacgtgctgc tggtcgactg tgcgccgggc gccggcagcg cggtgctgag
     7561 cctgctgcag gcggccagtg aggtgctgct ggtggtcaac gacgagccgg cggccctgtc
     7621 cgatgccctg gccctgatgc ggcgcctgaa ccagcggcac ggcatgaccc gtttccgcct
     7681 gctggccagc atgaccttcg cccagcaggc cggagaggcc ctgcacgagc gtctgctgcg
     7741 gctgtccgag ccgctggccg ggctgtcgct ggattatgtc ggcgcgattc ccttcgacga
     7801 ctcgctgcgc cgcgccgtgc agcgccagcg cccgctgctc gaggtgtttc cgcgcagccg
     7861 cgcttcgcag gcctacgccg cggtggccga gaaggtcgat ggctggccgc tgccggccaa
     7921 cccgcgcggc catctggagt tcttcgtcga gcggctgatc gcggcgcagg ccatcccgcg
     7981 cggctgaggc tcgggagtcc ctggggatga gctgcgacag ccggtcgcag ctcatcctta
     8041 agtcagtcat aagctgtcga aaatacgatc tccgggcgtt taggggcgca tgtataatgc
     8101 cgcccgactc cgctcgggcc atgtcccgag cgatgctttc gattgctcca ccgccgggta
     8161 gccatgcagt gaccagtccc tttctcgaag ccgttgcgct cgcctgcgag cgggatctgc
     8221 gcgtgctgtt cgagcgactc gacttcgccc tgcacgccgg cgagatgctg cagatcgccg
     8281 ggcccaacgg cagcggcaag accagcctgc tgcgcctgct cgccggtctg atgcagccga
     8341 ccgccggcga gatccgcctg gccggaaggc ccttggctga gcagcgtggc gagctgactc
     8401 gcaacctgct gtgggtcggc catgccgccg ggatcaaggg cctgctcagc ccggaagaaa
     8461 acctcgcctg gctgtgcgcc ctgcacaccg cggaaacccg cgaggcgatc tggcgcgcgc
     8521 tgcaggcggt cggtctgcgc ggcttcgagg acgtgccctg ccataccctg tccgccggcc
     8581 agcagcgtcg cgtcgccctg gcccgcctgt atctcgatcc gccgccgctg tgggtactcg
     8641 acgaaccctt cactgccctc gacaagcacg gcgtggccca gctcgaggag cacctggccc
     8701 gccactgcga gcagggcggc atggtggtgc tgaccaccca ccataccctc acgcgcaagc
     8761 cggccggcta ccgcgagctg gatctggccc aggccgggag cactgccgat gagtaacgtg
     8821 ttcacccagc tgctgacccg cgaggcgcgc ctgctgttcc gccgcccggc cgatctgctc
     8881 aatccgctgg tattcttcgg catcgtggtg gcgctgttcc cgctggccgt gggaccggag
     8941 agcaagctgc tggaaaccct gtcgccgggc ctggtgtggg tcgccgcgct gctcgccgtg
     9001 ctgctgtcgc tggatggcct gttccgcagc gacttcgagg acggctcgct ggagcagtgg
     9061 gtcctgtcgc cgcatccgct gccgctgctg gtgctggcca aggtgctggc ccactggctg
     9121 ttttccggtc tggcgctggt gctgctggca cccctgctgg cgctgatgct cggcctgccg
     9181 gcgcgctgcg tgccggtact gctgctctcc ttgctgctcg gcacgccgat cctcagcctg
     9241 ctcggcgcgg tcggcgcggc gctgaccgtg ggtctcaagc gcggcggcct gctgctggcg
     9301 ctgctgatcc tgccgctgta catcccggtg ctgatcctcg gcagcggtgc cctgcagtcg
     9361 gccctgcaag gattgccggt ggccggccac ctgttgtggc tggccagcct taccgccctg
     9421 gcggtgaccc tgactccctt tgccatcgcc gccggcctga ccatcagtgt cggcgaatga
     9481 atgaacacac gcgcagcagt ttcaaggcgg tttctatgaa ttggacgtgg tttcacaagc
     9541 taggctcgcc caagtggttc tacgacatca gcggccgctg gctgccctgg ctgggcgcgg
     9601 cggcagcgct gctgatcgcc gtcggcacgg tgtgggggct ggccttcgcg ccgccggact
     9661 accagcaggg caacagcttc cggatcatct acatccacgt gccggcggcg atcctcgccc
     9721 agtcctgcta catgatgctg gcggtggccg gcgtggtcgg cctggtgtgg aagatgaagc
     9781 tggccgacgt cgccctgcag tgcgccgcgc cgatcggcgc ctggatgacc ttcatcgccc
     9841 tggtcaccgg cgcggtgtgg ggcaagccga cctggggcgc ctggtgggtc tgggatgcgc
     9901 gcctgaccgc gatgctgatc ctgctgttcc tctacttcgg ggtgatcgcc ctcggccagt
     9961 cgatcagcaa ccgcgacagc gcggccaagg cctgcgcggt gctggccatc gtcggggtga
    10021 tcaacatccc gatcatcaag tactcggtgg agtggtggaa caccctgcac cagccggcga
    10081 ccttcacgat caccgagaaa ccggcgatgc cggcggagat gtgggtgccg ctgctgatca
    10141 tggtcatcgg tttctactgc ttcttcgccg tcgccctgct gctgcgcatg cgcctcgagg
    10201 tgctcaagcg cgagtcgcgg gccagctggg tcaaagccga agtggagcgc agcctgtgag
    10261 tttcgagtcc ttcgccgatt tcctcgccat gggcaagcat ggcctgtacg tctggacctc
    10321 ctacggcatc agcctggcgg tcctggccct aaacgtggcg ctgccgatcc tggcgcgccg
    10381 gcgttacctg cacgacgagg cgcgccgtct gcgccgggag agcaagcaat gaatccggta
    10441 cgcaagaaac gcctgttcat catcctcggc atcctcgccg gggtcggcgt ggccgtcggc
    10501 ctggcgctca gcgccctgca gcagaacatc aacctgttct acacgccgac gcagatcgcc
    10561 gccggcgagg cgccgcagga cacccgcatc cgcgccggcg gcatggtcag ggagggctcg
    10621 gtgagccgct cggccgactc gctagacgtg cagttcgtgg tcaccgactt cgccaaggac
    10681 gtgaccatcc agtatcgcgg catcctcccc gacctgttcc gcgaagggca gggcatcgtc
    10741 gcgctcggcc ggctgaacga ggagggcgtg ctggtggccg acgaggtgct ggccaagcac
    10801 gacgagaagt acatgccgcc ggaagtgacc aaggcgctga aggacagcgg caagctgccg
    10861 gaaggcagca tgccggccag cgccggcgcc aagatgggag gcgcgcaatg atccccgaac
    10921 tcggccacct ggccatgatc ctcgccctgt gcctggccgt ggtgcaggcg agcctgccgc
    10981 tgatcggcgc ctggcgcggc gatcgccagt ggatgggact ggcccggccg gcggcctggg
    11041 ggcagttcgc cttcctgctg ttttccttcc tgtgcctgac ccaggccttc ctggtcgacg
    11101 acttctcggt ggcctacgtc gccaagaact ccaacagcgc gctgccgtgg ttctacaagt
    11161 tcagcgcggt gtggggcgcc cacgagggct cgctgctgct gtgggcgctg atcctcggcg
    11221 cctggacctt cgccgtgtcg atcttctcgc gccagctgcc ggaagtgatg ctggcccgcg
    11281 tgctgtcggt gatgggcatg atcagcgtcg gcttcctgct gttcctgatc gtcacctcca
    11341 acccgttcga gcgcctgctg cccaacgcgc cgaccgacgg ccgcgacctc aacccgctgc
    11401 tgcaggatat cggcctgatc gcccacccac cgatgctgta catgggttac gtcggcttct
    11461 cggtggtgtt cgccttcgcc atcgccgcgc tgctcggcgg ccgcctcgac gcggcctggg
    11521 cgcgctggtc gcggccgtgg accatcgtcg cctgggcctt cctcggcatc ggcatcgtgc
    11581 tcggctcctg gtgggcctac tacgagctgg gctggggcgg ctggtggttc tgggacccgg
    11641 tggaaaacgc ctcgctgatg ccctggctgg tcggcacggc gctgctgcac tcgctggcgg
    11701 tgaccgagaa gcgcggcgtg ttcaagagct ggacggtgct gctggccatc gccgccttct
    11761 cgctgagcct gctcggcacc ttcctggtgc gctccggcgt gctgacctcg gtacacgcct
    11821 tcgccgccga ccccgagcgc ggcaccttca tcctgatctt cctgctgttc gtggtgggca
    11881 gctcgctgac cctgttcgcc ctgcgcgcgc cggtggtcaa gagcaacatc ggtttcggtc
    11941 tgtggtcgcg cgagacgctg ctgctggcca acaacctggt gctggtggtg gcctgtgcga
    12001 tggtcctgct cggcaccctg tatccgctgg tgctcgatgc gctgaccggc gccaagctgt
    12061 cggtcggccc gccgtacttc aatgcgctgt tcctgccgct gatggccatc ctcatgctgg
    12121 ccctcggtgt cggcgtgctg gtgcgctgga aggacacccc gctgaagtgg ctgagcggca
    12181 tgctgctgcc ggtgctggtg gtcagcggtg cgttcggcct gctcggcgcc tggctgttcg
    12241 gcgacttcca ctgggcggtg ctggccacct gcctgctggc cgcctgggtg gtgctcgccg
    12301 ggctgcgcga cctgctcgac aagacccgcc acaagggcct gctcaagggc atcgccggcc
    12361 tgtcgcgcag ctactggggc atgcagatcg cccacctggg catcgccgtc accgccctcg
    12421 gcgtggtgct gtccagccag tacgccctgg agcgcgacct gcgcatggcg ccgggcgagt
    12481 cggtcgaagt cggcggctac cagttcgtct tcgacggcgc ccagcaccat caggggccga
    12541 actacacctc tgatcgcgcc accgtgcacg tgctgcagga cggccgcgag atcaccgtgc
    12601 tgcatccgga gaagcgtgtg tacccggtgc gcggcatgcc gatgaccgag gccggcatcg
    12661 acccgggctt tacccgcgac ctctacgtcg cgctcggcga gccgctggaa gacggtgcct
    12721 gggccgtgcg cctgcacgtc aagccgttcg tgcgctggat ctggctgggc ggcctgctga
    12781 tgggcttcgg cggcttcctc gccgcgctcg accgccgcta ccgggtcaag gtgaccacca
    12841 aggcccgcga ggccctgggc atgactggag ctcaagcatg aaacgcgcaa tcctgctgct
    12901 gccgctggcg ctgttcctgg gcctggcggt gttcctctac cgcggcctgt tccttgatcc
    12961 ggccgagctg ccctcggcgc tgatcggcaa gccgttcccc gcgttcgcgc tgccgtcggt
    13021 gcacgactcc gcgcgcaccc tcagcgccgc cgacctcaag ggccggcctg cgctggtcaa
    13081 cgtctgggcg acctggtgcc cgacctgcaa ggccgagcac cagatgctca accagctggc
    13141 cggacagggc gtgaacatca tcggcgtcaa ctacaaggac gacaacgccg cggcgcgcaa
    13201 gtggctcaag gacttcctcg atccctatca gctcaacatc gaggacaagg tcggcagtct
    13261 gggcctgaac ctcggcgtct acggcgcgcc ggagaccttc ctgatcgaca aggacggcat
    13321 catccggcac aagtacgtcg gcgccatcga cgagcgcgtc tggcgcgagc agctggcccc
    13381 gctgtaccaa gaactggtgg acgcacgctg atgaaacgac tgctgagcaa acgctggctg
    13441 gctgggggcc tgctgggcct gaccctgtcg ctgggcgccc aggccgccat cgacacctac
    13501 gagttcaagg acgagggcga ccgtgcgcgc taccgcctgc tcaccgagga gctgcgctgc
    13561 ccgaagtgcc agaaccagaa catcgccgac tccgatgcgc cgatcgccat ggacctgcgc
    13621 cgcgagatcc accgcatgct tggcgagggc aagagcaacg acgagatcgt cgacttcctg
    13681 gtcgcccgct acggcgactt cgtgcgctac aacccgccgg tcaacgcgcg cacctggctg
    13741 ctctggtacg gaccgtggct gctgctcggc ggcggtctcg gcgtgctggc gctgatcgtc
    13801 ctgcgccgcc gccgtcccgc ggcgtccggc cagggtgccg acctctccgc cgaggagcgc
    13861 gcgcgcctcg accaactgct gaagaactct gttcacaaga gctcggccga gcagagctcc
    13921 gccgacaacc ccaaggctga taaatgaccg ctttctggct tgccgccgca gcgctgctgc
    13981 tggtggcgtt cgctttcctg ctgattccgc tgctgcgcgg ccgtcgtgcc caggccgagg
    14041 aagaccgcac cgccctcaac gtcgccctgt accaggagcg tgtcgccgaa ctggacgccc
    14101 agcacgccgc cggcgccctc gatgccgccc aacgcgacgc cggccgccgt gaggccgccc
    14161 gcgagctgct ggatgatgcc gagcaggccg aaggcgcgca gcgcaagctg ggccgctggt
    14221 tgccgctggc gatggcgctg ctgatgccgg tgctcggcct tggcctgtac ctgcactggg
    14281 gcgccagcga tgcgctcaag ctgagcgagc agctggccac cgagccgcag aacatcgagg
    14341 aaatgaccgc ccgtctcgag gaaacggtgc gtctgcaacc ggatcaggcc gaagcctggt
    14401 acttcctcgg ccgtacctac atggcccagc agcgacccaa ggaggccgca ccggccttcg
    14461 ggcgtgccgt cgagctggcc ggccgccagc cggagctgct cggccagtgg gcgcaggcgc
    14521 tgtacttcgc cggcggacag aagttcaccg acgaggtgcg cagtctcggc gaggaaacgc
    14581 tgcgcctcga tccgcaggag cccaccaccc tcggtctgct cggcatcgcc gctttcgagg
    14641 agcagcgttt cgtcgacgcg gtgcgctatt gggaacgtct ggtcgccacg ctgcaaccga
    14701 acgacccgtc gcgcg
//
LOCUS       DATCUN010000164         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_137684, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000164 DATCUN010000000
VERSION     DATCUN010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137684"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2188
                     /gene="nirB"
                     /locus_tag="VIO83_06720"
     CDS             <1..2188
                     /gene="nirB"
                     /locus_tag="VIO83_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HEY8331001.1"
                     /translation="RKVIAADGSEATYDRLIIATGSNPFILPVPGNDLDGVIGYRDIA
                     DTEMMMATAKTHRHALVIGGGLLGLEAANGLKLRGMDVTVVHIGDWLLDRQLDKTAGQ
                     LLQTALEARGIQFRLNTHTAALLDNGEGRVCAAQFKDGEVIPADLVVMAAGIRPNTEL
                     AERAGIACNRGILVSDTLQTFDPRIYAVGECASHRGIAYGLVAPLFEQAKVCANHLAQ
                     FGIGMYQGSVTSTKLKVTGIDLFSAGDFMGEDGTETITLSDPIGGVYKKLVVKDDILV
                     GACLYGDTADGGWYFRQVRERANISEIRDHLMFGENTIGDVGHQGQHSTANMPDDMEV
                     CGCNGVCKGTIVKAIQENGLFSVDEVKKHTKAASSCGSCTGLVEQILISTVGGAADVK
                     PKSEKAICGCSDLNHGQVRKAIRDHHLTAMADAMAFMEWRTPNGCATCRPALNYYLIS
                     TWPGEARDDAQSRLINERAHANIQKDGTYSVVPRMWGGVTNAAELRRIADVADKYNVP
                     MVKVTGGQRIDLLGIKKEDLPGVWQDLDMPCGHAYGKSIRTVKTCVGSEFCRFGTQNS
                     TQMGIDLEHALFNMWSPHKVKLAVSGCPRNCAESGIKDVGIIGVDSGWELYIGGNGGI
                     KTEAGEFFVKVKTAEEVMEYSLAFLQLYREEAFYLERTVHYLHRVGMEHIKQAVLEDA
                     ERRKDLHARLLFSLSFEQDPWQQQVATPALKKEFERIPVVLEETTR"
     gene            2185..>2279
                     /locus_tag="VIO83_06725"
     CDS             2185..>2279
                     /locus_tag="VIO83_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase (NAD(P)H) small subunit"
                     /protein_id="HEY8331002.1"
                     /translation="MNWLDICALEEINVLGARVVAGPKGDIALFRT"
BASE COUNT          423 a          794 c          734 g          328 t
ORIGIN      
        1 ccgcaaggtg atcgccgccg acggcagcga ggccacctac gaccgcctga tcatcgccac
       61 cggctccaac ccgttcatcc tgccggtccc cggcaacgat cttgacggtg tgatcggcta
      121 ccgcgacata gccgataccg agatgatgat ggccaccgcc aagacccacc gccatgcgct
      181 ggtgatcggt ggcggcctgc tcggtctgga ggcggccaac ggcctcaagc tgcgcggcat
      241 ggacgtcacc gtggtgcaca tcggcgactg gctgctcgat cgccagctgg acaagaccgc
      301 cggccagctg ctgcagaccg ccctggaggc ccgcggcatc cagttccgcc tcaacaccca
      361 caccgccgcc ctgctcgaca acggcgaggg ccgggtgtgc gccgcccagt tcaaggatgg
      421 cgaggtgatc cccgccgacc tggtggtgat ggccgccggc atccgcccca acaccgaact
      481 ggccgagcgc gcgggcatcg cctgcaaccg cggcatcctg gtcagcgaca ccctgcagac
      541 cttcgatccg cgcatctacg cggtcggcga gtgcgccagc caccgcggca tcgcctacgg
      601 cctggtcgcc ccgctgttcg agcaggccaa ggtctgcgcc aaccatctgg cccagttcgg
      661 catcggcatg taccagggct cggtgacctc gaccaagctg aaggtgaccg gcatcgacct
      721 gttctccgcc ggcgacttca tgggcgagga cggcaccgag accatcaccc tctccgaccc
      781 gatcggcggc gtgtacaaga agctggtggt caaggacgac atcctggtcg gcgcctgcct
      841 gtacggcgac accgccgacg gcggctggta tttccgccag gtgcgcgagc gcgccaacat
      901 cagcgagatc cgcgaccacc tgatgttcgg cgagaacacg atcggcgacg tcggccacca
      961 gggccagcac agcaccgcga acatgcccga cgacatggag gtgtgcggct gcaacggcgt
     1021 gtgcaagggc accatcgtca aggccatcca ggaaaacggc ctgttcagcg tcgacgaggt
     1081 gaagaagcac accaaggccg ccagctcctg cggctcctgc accggcctgg tcgagcagat
     1141 cctgatcagc accgtcggcg gcgccgcgga cgtcaagccc aagagcgaga aggccatctg
     1201 cggctgcagc gacctcaatc acggccaggt gcgcaaggcg atccgcgacc atcacctgac
     1261 cgcgatggcc gatgccatgg ccttcatgga gtggcgcacc cccaacggct gcgccacctg
     1321 ccgcccggcg ctgaactact acctgatctc cacctggccg ggcgaggcca gggacgatgc
     1381 gcagtcgcgc ctgatcaacg agcgcgccca cgccaatatc cagaaggacg gcacctactc
     1441 ggtggtgccg cgcatgtggg gcggggtgac caacgcggcc gagctgcggc gcatcgccga
     1501 cgtggccgac aagtacaacg tgccgatggt caaggtcacc ggcggccagc gcatcgacct
     1561 gctcggcatc aagaaggaag acctgccggg cgtctggcag gacctggaca tgccctgcgg
     1621 ccacgcctac ggcaagtcca tccgtaccgt gaagacctgt gtcggcagcg agttctgccg
     1681 cttcggcacg cagaactcga cgcagatggg catcgacctg gagcacgcgc tgttcaacat
     1741 gtggtcgccg cacaaggtca agctggcggt ctccggttgc ccgcgcaact gcgccgagtc
     1801 ggggatcaag gacgtcggca tcatcggcgt ggattccggc tgggagctgt acatcggcgg
     1861 caacggcggc atcaagaccg aggccggcga gttcttcgtc aaggtcaaga ctgccgagga
     1921 agtcatggag tacagcctgg ccttcctgca gctgtaccgc gaggaggcgt tctacctgga
     1981 gcgcaccgtg cactacctgc accgcgtcgg catggagcac atcaagcagg ccgtgctgga
     2041 ggacgcggaa cgccgcaagg acctgcacgc ccgcctgctg ttctcgctgt ccttcgagca
     2101 ggacccgtgg cagcagcagg tcgccacccc ggcgctgaag aaggagttcg agcgcatccc
     2161 cgtcgtcctt gaggagacca cccgatgaac tggctggata tctgtgccct ggaagagatc
     2221 aacgtgctcg gcgcgcgcgt cgtcgccggc cccaagggcg acatcgccct gttcaggac
//
LOCUS       DATCUN010000165         4032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_101134, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000165 DATCUN010000000
VERSION     DATCUN010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101134"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1790)
                     /locus_tag="VIO83_06730"
     CDS             complement(<1..1790)
                     /locus_tag="VIO83_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY8331003.1"
                     /translation="MQHTVSQAVAGEPHRQTSEEVLAAFRTDAHAGLTGQEALLRIQG
                     HGANTLAEEAQPSAWWKFLAQFTDVLVVLLLVAALISAGLWLVERDSALPFEALAIFA
                     IVLLNAIMGYLQEARAERAVAALQKLSAAQATVIRDGVRQRIPAAEIVPGDLLLVEEG
                     DTIAADARLIESISLQMAEAALTGESQPASKSIAPIPGEAPVGDRHNMIFSGTTVTYG
                     RGRAVVTATGMRTQIGRIAGMLESVPSETTPLQQELDRVGRLLGTIVVAIAVVMIATI
                     LMVEDVRGFSAVFDVLILGVALAVAAVPEGLPAIVTAVLSLGVQRMARRHAIVRHLAA
                     VETLGSANVIASDKTGTLTRNEMTVRILATASGRVVFGGTGYGPQGEVSREDGAALDE
                     DLRAELLRALTVADRASNAVLVEQDGRWTVQGDPTEGALIVAARKAGLDPATLGARFR
                     RIGEVPFSSERKLMSTVHLDAETSDRCLVLTKGAPDRVLARCSHEWVGQQAQALTAAR
                     REQIAAVNDALAGQALRTLGLAFRALPGDAAASEVFDEGLEQDLVFLGLAGMIDPPRA
                     EARQAIGKAKAAGIRPIMITGDHPVTAAVIA"
     gene            complement(2093..2488)
                     /locus_tag="VIO83_06735"
     CDS             complement(2093..2488)
                     /locus_tag="VIO83_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8331004.1"
                     /translation="MSKTITISLPVTDLDRSRAFYSAIGFEPNPDFSFPDAAMMMWSE
                     HIGVMLLTHDRWRTFTTRPIPPNTSSEVALNISCASRQEVDAMAEAAGQHGGTTDINP
                     VEEHGTMYGRDFCDPDGHVWGAMWMDARE"
     gene            complement(2886..3635)
                     /locus_tag="VIO83_06740"
     CDS             complement(2886..3635)
                     /locus_tag="VIO83_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331005.1"
                     /translation="MSEHAFDDKEMGYFLPEDSQLLLTQIKNHIHFLARLAQPRIADE
                     TKEWAPVVGMAELMFCLDQLAEQLDLVLQEVSWLEGRARTEETSEDAQDADDADDAVE
                     ADDGAEADDATEADDGAEADDGAPAGEDPFVFGVTMDQLDQLNLLVDRITAYGDAVSA
                     DDMADFAPGTLSVLGHTIFDRANELDEIVTEINAQHLEPGARLRFSVKETPPAYLAQA
                     PSEVDQAPRAVPALAYPLQAHSPPAGGLHLH"
BASE COUNT          643 a         1411 c         1266 g          712 t
ORIGIN      
        1 gcgatgaccg cggccgtcac cgggtggtcg ccggtgatca tgatcgggcg gatccccgcc
       61 gccttggcct ttccaatcgc ctgcctggcc tccgcgcgtg gcggatcgat catgccggcc
      121 agccccagga acaccaggtc ctgctccagc ccctcgtcga agacctcgct cgccgcggca
      181 tcgccgggga gcgcgcggaa tgcgagcccg agcgtgcgca gggcctgacc ggccagcgcg
      241 tcatttaccg ctgcgatctg ctcgcgacgg gccgcggtca gcgcctgcgc ctgttggccc
      301 acccattcgt gcgagcagcg cgccagcact ctgtccggtg cgcccttggt cagcaccagg
      361 caccgatccg atgtttcggc gtccaggtgg acggtgctca tcagcttgcg ttcggaggag
      421 aacggcacct cgccaatgcg cctgaagcgg gcgccgagcg tggccgggtc gagtccggcc
      481 ttgcgtgcgg ccacgatgag tgcgccttcg gtcggatcgc cctgcaccgt ccagcgcccg
      541 tcctgttcga ccaggaccgc gttgctggca cgatcggcca cggtcagggc gcggagaagt
      601 tcggcgcgca ggtcctcatc cagcgcagcg ccatcctcgc ggctgacttc gccctggggg
      661 ccgtagccgg ttccgccgaa gaccacccgt ccgctggcgg tggccaggat ccgcacggtc
      721 atctcgttgc gggtcagggt cccggtcttg tccgatgcga tcacgttcgc cgagcccagc
      781 gtttccaccg ccgccaggtg gcgcacgatg gcgtgccgcc tggccatgcg ctggaccccc
      841 agcgagagca ccgcagtgac gatcgccggc aggccctccg gcacggcggc cacggccagc
      901 gccacaccca ggatcaggac gtcaaagacc gcggaaaatc cgcgcacgtc ctcgaccatc
      961 aggatggtgg cgatcatcac cactgcgatc gccaccacga tggtgcccag cagccggcca
     1021 acccggtcca gctcctgctg cagcggcgtg gtctcgctcg gcacgctctc cagcatgccg
     1081 gcgatgcgtc cgatctgggt ccgcatgccg gtggccgtga ccaccgcgcg gccgcgccca
     1141 taggtgaccg tggtgccgga gaagatcatg ttgtggcgat cgccgaccgg ggcctcgccc
     1201 gggattggcg cgatgctctt gctcgccggc tggctttctc cggtcagtgc agcctcggcc
     1261 atctgcagcg agatcgattc gatcaatcgc gcgtcggccg cgatggtgtc gccctcctcg
     1321 accagcagga ggtcgccagg cacgatctcg gcggccggga tgcgctggcg cacgccatcg
     1381 cggatcaccg tggcctgggc cgccgacagc ttctgcagcg cggccaccgc gcgctcggcg
     1441 cgggcctcct gcaggtagcc catgatcgcg ttcagcagga cgatggcgaa gatcgccagc
     1501 gcctcgaacg gcaaggcgga gtcgcgttcg accagccaca gcccggccga gatcaacgcc
     1561 gccaccagca gcaggacgac cagcacgtcg gtgaactggg ccaggaactt ccaccaggcc
     1621 gacggctgcg cttcctcggc cagggtgttc gccccgtgcc cctggatccg caacaaggcc
     1681 tcctgcccgg tcagcccggc gtgcgcgtcg gtccgaaatg cggcgaggac ctcctcgctg
     1741 gtctgtcgat ggggttcgcc cgcaaccgcc tgggacaccg tgtgctgcat acccgtttcc
     1801 aaatccgtgc ccaggtcgat ggatcgaagc gatggccgga ggcaccgaac cgatgcgccg
     1861 cgtccggcgc gaaccgccgg accacccccg gtcaaacggc cgcaaggcca gccgactcta
     1921 gtcgacgcgg cgtgcattca ctcatcaccc gcggggggat cgtgccgctt tcgatggcag
     1981 ccggtgcagg ggcgagggat tggcaccacg cttcagcttg ccttcgcccg ggatcgcgtc
     2041 ccttttgcga gggcctctcg cacctggacc aggtgaagtg aacctgaccc cgttattccc
     2101 ttgcgtccat ccacatggcg ccccacacat gcccatccgg gtcacagaag tcgcggccgt
     2161 acatggtgcc gtgctcttcg accgggttga tgtcggtggt cccgccatgc tggcccgcgg
     2221 cttctgccat ggcatccacc tcctggcgtg atgcgcacga gatattcaat gcaacctcgc
     2281 tcgaggtgtt cggcgggatc ggccgcgtgg tgaacgtgcg ccacctgtcg tgcgtcagca
     2341 gcatgacgcc gatgtgttcg ctccacatca tcatggccgc atcgggaaag gaaaaatccg
     2401 ggttgggctc gaagccgatg gccgaataga acgccctgga ccgatcgagg tccgtcacag
     2461 gcaggctgat ggtgatggtc ttcgacatgc ggatgctcct gctgcgtggg ccgacgcctc
     2521 atcatccgcc atctgggctg gaagtgaacc tgaccccgtc aatcctctct aatcatctca
     2581 gcacgcgccg ctagaccgca tcacccaggc ccggcaagaa caacaaccac cccctgcctt
     2641 cctgtagagc ggagcttgct ccgctgctct tgtaaagcgg agcttgctcc gctgctcttg
     2701 tagagcggag cttgctccgc tggagcttta ccgaaattcc gaccgaagag cagcggagca
     2761 agctccgctc tacagggttc gcgtgtcacg cgggatggga cgcatcacgc gattcacctg
     2821 ccagcgccca acccgacccc gccggctttc ctccgaacga cgggatcggg ttccgaggca
     2881 tcggctcagt gcaggtgcag cccgcctgcg ggtgggctgt gagcctgcag tggatatgcc
     2941 aaagccggca ctgcacgcgg tgcctgatcc acctcggatg gtgcctgggc aagatacgca
     3001 ggcggcgtct ccttgaccga gaagcgcagg cgcgcgcccg gctccagatg ctgcgcgttg
     3061 atctcggtca cgatctcatc cagctcgttg gcccgatcga aaatcgtgtg ccccaggacc
     3121 gagagcgtac cgggggcaaa gtcggccatg tcgtcggcag acaccgcatc gccatacgcc
     3181 gtgatgcgat cgaccaagag gttgagctga tcgagctggt ccatcgtgac cccgaagaca
     3241 aaggggtctt cgccagccgg ggccccatcg tcggcttcag ccccatcgtc ggcttcagtc
     3301 gcatcgtcgg cttcagcccc atcgtcggct tcaaccgcgt cgtcggcatc gtctgcgtcc
     3361 tgcgcatcct cggaggtttc ctcggtccgc gcccgccctt ccagccacga cacctcctgc
     3421 agcaccaggt ccaactgctc ggccagctgg tccaggcaga acatcaactc cgccatgccc
     3481 accacgggcg cccactcttt cgtttcatcc gcaatgcgcg gctgtgccag cctcgccagg
     3541 aagtggatgt ggttcttgat ttgcgtaagc aacagctggc tgtcttccgg caggaaatac
     3601 cccatctcct tatcgtcgaa cgcgtgctca gacatcgtcg tctccttctc accatgaagt
     3661 gcccgcccgc gctgatgcga cggacgggaa gtcgggaggc tagaaaccgc gtacagacgg
     3721 cgggcgtatt ccccttgcgg gtgttgtatt agccgccctc ccgacgcgga gcattgcgtc
     3781 ggcttgtccc tgcggaatgc aggcacaaaa aacccaccgg tttgacggag gtgggtaccg
     3841 ctgtacgagg agtttctagg ctccttgaac ccgagattgc taccacaagc cgcctgtgtc
     3901 aacacctgga ttgagcacca atgccctgcc ccagaatgac ggcaaagttc gctttgctgt
     3961 tgccgctgct gttgccgctg ctgttgccgc tgctgttgcc gctgctgttg ccgctgctgt
     4021 tgccgctgct gt
//
LOCUS       DATCUN010000166        36914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_119489, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000166 DATCUN010000000
VERSION     DATCUN010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 36914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36914
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119489"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="VIO83_06745"
     CDS             <1..1253
                     /locus_tag="VIO83_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009867811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEY8331006.1"
                     /translation="GQESWSEEDIRASRQLLREATTAQAPVYQTDPLGLMLVVETGDS
                     HITILDGDKLEPFHRFATRAQLHGSPKFTRDGRYAFFVSRDGWVTKYDLWTLEVVAEV
                     RAGLDTRNIAVDPTGDHVAVANYQPHTLVLLDGDLNLLKSMPALDQKAEHSSRVSAVY
                     SVPSRRSFVVALKDIAELWEVSYDPQAEDMAIGLVHDFQYREGTFVTGYLNPRRIELD
                     APLDDFFFNPDQAELFGVSPQGEVQVIQLDARQRIGGLPLQGRPHMGAAVTWSRQGVT
                     LMASTNLKLPEVAVVDPQRRELVQRIALQGPGFFLAGHENSRYLFVDSMLAADSRHVL
                     QAIDRETLEVARRFAAEPGDTLAHVEFTRDGRYALASLWRDDGAVIVFDTQSLEEVKR
                     LPMKRPVGKYSVWRGIESQQGAAR"
     gene            1375..5271
                     /gene="purL"
                     /locus_tag="VIO83_06750"
                     /gene_synonym="purI"
     CDS             1375..5271
                     /gene="purL"
                     /locus_tag="VIO83_06750"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEY8331007.1"
                     /translation="MLILRGAPALSAFRHGKLLAQLTEKVPAVSGLYAEFAHFAEVSG
                     ALGAEEQQVLTRLLKYGPSVPMQEPAGRLFLVVPRFGTISPWSSKASDIAHNCSLEKI
                     QRLERGIAYYVQGELSDSDAELVAAALHDRMTQLVLGRFEEAANLFSHAQPKPLTAVD
                     VLGGGRAALEKANVELGLALAEDEIDYLVQAFTGLGRNPHDIELMMFAQANSEHCRHK
                     IFNASWDIDGESQDKSLFGMIKNTYQLHNEGVLSAYKDNAAVIVGNVAGRFYPNPETR
                     EYAASQEPVHILMKVETHNHPTAIAPFPGASTGSGGEIRDEGATGRGAKPKAGLTGFT
                     VSNLNIPGFEQPWEKPYGKPERIVTPLDIMIEGPLGGAAFNNEFGRPALTGYFRTFEQ
                     AIDTPRGEEVRGYHKPIMLAGGMGNIREDHVQKGEISVGGKLIVLGGPAMLIGLGGGA
                     ASSMATGTSSADLDFASVQRDNPEMERRCQEVIDRCWQLGERNPIKFIHDVGAGGLSN
                     AFPELVNDGGHGGRFELRNIPNDEPGMAPHEIWCNESQERYVLSVDAADLERFAAICE
                     RERCPFAVVGEATEEAHLTVADSHFGNKPVDMPLNVLLGKAPRMHRSAQREAELGDDF
                     NAASVYLDEAVGRVLRHPAVASKSFLITIGDRSITGLVARDQMVGPWQVPVADCAVTA
                     ASYDVYTGEAMAMGERTPLALLDAPASGRMAIGETLTNLAAARIEKLSDIKLSANWMA
                     AAGHPGEDARLYDTVKAVGMELCPALGITIPVGKDSMSMKTRWQDAGADKSVTAPMSL
                     IVSGFAPVQDIRRTLTPQLRLDQGASELLLIDLGRGQNRLGGSILAQVYSQIGRSVPD
                     VDKAEDLKGFFDTIQGLNADGKLLAYHDRSDGGLLTTVVEMAFAGHCGLDLQLDVLAA
                     SRAALGAALFSEELGAVVQVLQTDTAEVLARFAAVGLADCVSVIGQPVAGGDIRLGLG
                     GDVIYSAERRILQRIWSETSYRIQRMRDNAQCAEQEFDLLLDESNPGLSVKLGFDVND
                     NVAAPYINTGVRPRMAILREQGVNGQTEMAAAFHRAGFAVVDVHMSDIIAGRVSLEDF
                     KGLVACGGFSYGDVLGAGGGWAKSVLFNNRARDNFSAFFERKDSFALGVCNGCQMLSN
                     LGELIPGSEAWPRFVRNRSEQFEARVAMVQVQESASIFLQGMAGSRLPIAIAHGEGHA
                     EYASEEALLQADLSGAVALRFVDNHGKVTESYPANPNGSPRGITGLTTRDGRVTIMMP
                     HPERVFRAVQNSWRPDDWQEDAGWMRMFRNARVWVD"
     gene            5520..5837
                     /locus_tag="VIO83_06755"
     CDS             5520..5837
                     /locus_tag="VIO83_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqfO family protein"
                     /protein_id="HEY8331008.1"
                     /translation="MYKLCFFVPESHLETVKTAIFATGAGRIGSYDSCCWQTLGQGQF
                     HPLPGSQPYEGAQGELAQVAEWKVEMVVADELIHAAVKALKQNHPYETPAFDVWRLSD
                     MVF"
     gene            complement(5932..6435)
                     /locus_tag="VIO83_06760"
     CDS             complement(5932..6435)
                     /locus_tag="VIO83_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY8331009.1"
                     /translation="MRWSIVFCCFLFATLANAMSTPGLDRPAVDKVLVVKSERKLLLL
                     SRGEVLKSYRVSLGKQPNGPKRYQGDKRTPEGFYWIDWRKASDKYNLSMHISYPNQQD
                     LHHARQKGLPAGDMIMIHGTPLDEEYPEWYFHTLDWTEGCIAMRNHDIREVWSLVKDG
                     TLIEIRP"
     gene            6674..7276
                     /locus_tag="VIO83_06765"
     CDS             6674..7276
                     /locus_tag="VIO83_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY8331010.1"
                     /translation="MLDELRQRVQAHSPGSLPSDRNFPEAAVLMPITRSDDPQLVLTL
                     RASGLSTHGGEVAFPGGRRDPEDESLVFTALREAYEEVGLAPGLVEVVGPLSTQISRH
                     GIKVTPYVGFIPDYVEFKANDDEIAAVFSVPLEFFRDDPREITHRIDYLGYSWYVPSY
                     RYGGFKIWGLTAMMVVELVNLIYDAGIELREPPPHFSHYR"
     gene            7297..7821
                     /locus_tag="VIO83_06770"
     CDS             7297..7821
                     /locus_tag="VIO83_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY8331011.1"
                     /translation="MKYHLGDARVEAHPDSWIAPTASVIGKVRLEAGASVWFGAVLRG
                     DNELIHVGENSNVQDGSVLHTDMGWPLTIGKGVTIGHKVNLHGCSIGDHSLIGINAVV
                     LNGAKIGRHCLIGAGALIPEGKEIPDGSLVMGVPGKVVRSLSEEQMRGLENNAAHYVD
                     NARRYARELAEQAD"
     gene            7821..8048
                     /locus_tag="VIO83_06775"
     CDS             7821..8048
                     /locus_tag="VIO83_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEY8331012.1"
                     /translation="MAERQQDNVQEKPLASPCVYVCALDDDDICIGCQRSGEEITRWG
                     RMDNAERRAVLVRCQERALAAGQFLVKPNPL"
     gene            8061..9242
                     /gene="purT"
                     /locus_tag="VIO83_06780"
     CDS             8061..9242
                     /gene="purT"
                     /locus_tag="VIO83_06780"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HEY8331013.1"
                     /translation="MTMIGTPLSPSATRVLLCGSGELGKEVAIELQRLGCEVIAVDRY
                     ANAPAMQVAHRSHVISMLDGAALRAVIEQEQPHYIVPEIEAIATDTLVELEAEGFTVV
                     PTARAARLTMNREGIRRLAAEELGLPTSPYRFADSFADYAAAVAELGFPCVVKPIMSS
                     SGKGQSLLRGEADVQQAWDYAQEGGRAGKGRVIVEGFIDFDYEITLLTVRHAGGTTFC
                     APVGHRQEKGDYQESWQPQAMSPKALAESERIAHAVTESLGGRGLFGVELFIKGEQVW
                     FSEVSPRPHDTGLVTLISQDLSEFALHARAILGLPIPAIRQFGPSASAVILVEGESKE
                     VRFGNLGAALAEADTALRLFGKPEVSGQRRMGVALARDASIETARAKALRAAQAVTIQ
                     L"
     gene            complement(9314..10081)
                     /locus_tag="VIO83_06785"
                     /pseudo
     CDS             complement(9314..10081)
                     /locus_tag="VIO83_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019461.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
     gene            complement(10192..10986)
                     /gene="ccsA"
                     /locus_tag="VIO83_06790"
     CDS             complement(10192..10986)
                     /gene="ccsA"
                     /locus_tag="VIO83_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY8331014.1"
                     /translation="MTPLLPSLAAAGLYAGAALYQALLLSRRATPDKRLLGLLGVLAL
                     LAHAFGLRLLLNNGALQLDFFNASSLLAAAVIALTLLASLRIPVENLLVLLFPLGSLT
                     TLLALLPGGTTQPIVEEPGILAHILLSILAYGLLTIAVVQALLLLLQNRQLKNKHPSG
                     IIRNFPPLQTMESLLFGFLWGGWSLLSLSLLSGWLFLDDLFAQHLVHKTLLACVAWVV
                     FGVLLWGRHQLGWRGGQAIRWTLAGFLLLMLAYFGSKLVREFILHI"
     gene            11129..12505
                     /gene="ffh"
                     /locus_tag="VIO83_06795"
     CDS             11129..12505
                     /gene="ffh"
                     /locus_tag="VIO83_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY8331015.1"
                     /translation="MFENLTDRLSQTLRNVTGRAKLTEDNIKDTLREVRMALLEADVA
                     LPVVKDFVNKVKDRAVGTEVSKSLTPGQAFVKIVRAELEELMGAANEDLNLAATPPAV
                     ILMAGLQGAGKTTTVGKLARFLKERKKKSVLVVSADVYRPAAIKQLETLANEVGVTFF
                     PSDLSQKPVDIARAAIAEGKLKFIDVVIVDTAGRLHIDAEMMDEIKQVHAAINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSVRAITGKPIKFLGMG
                     EKSEALDPFHPDRVASRILGMGDVLSLIEQAEQTMDREKAEKLTKKLKKGKGFDLEDF
                     RDQLQQMKNMGGLGSLMDKLPMLGGMNLGQMGNAQNVAEKQFKQMEAIINSMTPLERR
                     DPEVISGSRKRRIALGSGTQVQDVGRLIKQHKQMQKMMKKVTAKGGMAKMMRGMSGLL
                     PGGGMPKM"
     gene            12727..12972
                     /gene="rpsP"
                     /locus_tag="VIO83_06800"
     CDS             12727..12972
                     /gene="rpsP"
                     /locus_tag="VIO83_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY8331016.1"
                     /translation="MVTIRLARGGSKKRPFYHLTVTNSRSAREGRFIERIGFFNPVAT
                     GGEVRLSVNAERAQYWLSQGAQASERAAQLLKEAAKA"
     gene            12983..13519
                     /gene="rimM"
                     /locus_tag="VIO83_06805"
     CDS             12983..13519
                     /gene="rimM"
                     /locus_tag="VIO83_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019460.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY8331017.1"
                     /translation="MQHSPAPAEDLVVLGKIVSVFGIRGEVKVYSYTDPLENLLDYRH
                     WTLKRDGEVRQVELVSGRLHGKVLTVRIKGLDDREEALSHVGFLILVPRSQLPALQDD
                     EIYWYQLEGLRVINQDGQLLGRVDHLLETGANDVLVVKPCAGSLDDRERLLPYTGQCV
                     QQVDLAAGEMRVDWDADF"
     gene            13523..14266
                     /gene="trmD"
                     /locus_tag="VIO83_06810"
     CDS             13523..14266
                     /gene="trmD"
                     /locus_tag="VIO83_06810"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY8331018.1"
                     /translation="MRVDVLSLFPEMFGAISEYGITSRAVKQGLLQLHCWNPRDYTED
                     RHQTVDDRPFGGGPGMVMKIKPLELALASAKTAAGGAAKVIYLSPQGRRLDQAAVREL
                     AKEERLILIAGRYEGIDERFIETHVDEEWSIGDYVLSGGELPAMVLIDAVTRLLPGAL
                     GHADSAEEDSFTDGLLDCPHYTRPEVYEGRSVPPVLLSGNHEHIRRWRLQQSLGRTWE
                     RRADLLDSRSLSGEEKKLLEEYIRQRDDR"
     gene            14309..14665
                     /gene="rplS"
                     /locus_tag="VIO83_06815"
     CDS             14309..14665
                     /gene="rplS"
                     /locus_tag="VIO83_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY8331019.1"
                     /translation="MSNKNLIIQQIEAEQMTKAIPAFAPGDTVIVQVKVKEGDRERLQ
                     AFEGVVIAKRSRGLNSAFTVRKISNGFGVERTFQTYSPLVDSINVKRRGDVRKAKLYY
                     LRALSGRAARIKEKLV"
     gene            14798..15694
                     /gene="xerD"
                     /locus_tag="VIO83_06820"
     CDS             14798..15694
                     /gene="xerD"
                     /locus_tag="VIO83_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY8331020.1"
                     /translation="MPAPDHPLIDRFLETLWLEKGLSRHTRSAYGSDLALFQGWLVAR
                     GFDLLRVDRELIREHLGWRLDNGYHARSTARLLSCLRGFYHFCSFEGLLAEDPTLLVS
                     LPRQARAVPSSLSEADVEALLAAPEVSEALGLRDRAMFEVLYACGLRVSELVALTLEQ
                     VNLRQGVVRVFGKGARERLVPLGEEAIDWLLRYLREARGELLIGKPSDVLFPSLRGEQ
                     MTRQTFWHRIKLHARTAGIDKPLSPHTLRHAFATHLLNHGADLRVVQMLLGHRDLSTT
                     QIYTHVARARLQALHAEHHPRG"
     gene            15794..16540
                     /locus_tag="VIO83_06825"
     CDS             15794..16540
                     /locus_tag="VIO83_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HEY8331021.1"
                     /translation="MRLFRFLAAATFGLASSIALAAEPDQAIRQALKSLQPDLPIEAI
                     SESPMPGLYQVQLKGGRLLYTSADAQFVLQGFLFQVKDGKTVNLTEQAESKGVAKQIN
                     AIPQAEMVVFPAAEKAAQKTHITVFTDTDCGYCQKLHSEVAELNRRGVEVRYVAFPRQ
                     GTESHGYNTLVSVWCAKDRREAMNLAKARKDVPAAQCDNPVAKQYQLGQMIGVQGTPA
                     IVLANGRMIPGYQPAPQLAEAALAAAAAAK"
     gene            16650..17954
                     /locus_tag="VIO83_06830"
     CDS             16650..17954
                     /locus_tag="VIO83_06830"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY8331022.1"
                     /translation="MKPVKVGICGLGTVGGGTFNVLKRNAEEIARRAGRGIEVAQIAA
                     RDANPQCDTTGTPITGDVFEVACNPEIDIVIELIGGYTVARELVLKAIENGKHVVTAN
                     KALIAVHGNEIFAKAREKGVIVAFEAAVAGGIPVIKAIREGLAGNRINWLAGIINGTG
                     NFILTEMREKGRAFDDVLKEAQALGYAEADPTFDVEGIDAAHKLTILASIAFGIPLQF
                     DKAYTEGITQLTTADVNYAEALGYRIKHLGVARRTEAGIELRVHPTLIPADRLIANVN
                     GVMNAVMVNGDAVGSTLFYGAGAGMEPTASAVVADVVDVTRVLTTDPENRVPHLAFQP
                     DALSDHPILPIEACESAYYLRIQAKDHPGVLAQVATILSERGINIESIMQKEAEEQAG
                     LVPMILVTHRVRESQMNDAIVALEALEGVSGQVVRIRVEQLN"
     gene            18028..19437
                     /gene="thrC"
                     /locus_tag="VIO83_06835"
     CDS             18028..19437
                     /gene="thrC"
                     /locus_tag="VIO83_06835"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY8331023.1"
                     /translation="MRYISTRGQAPALNFEDVLLAGLASDGGLYVPENLPRFTVEEIA
                     SWAGLPYHELAFRVMRPFVAGCINDADFKKILEDTYGVFAHQAVAPLTQLGANEWVLE
                     LFQGPTLAFKDFALQLLGRLLDHVLAKRNERVVIMGATSGDTGSAAIEGCKACDNVDI
                     FIMHPHNRVSEVQRRQMTTILGENIHNIAIEGNFDDCQEMVKASFADQGFLKGTRLVA
                     VNSINWARIMAQIVYYFHAALQLGAPHRSVAFSVPTGNFGDIFAGYLARNMGLPINQL
                     IVATNRNDILHRFMSGNRYDKDTLHASLSPSMDIMVSSNFERLLFDLHGRNGLAVSAL
                     MDEFKATGKLSVEDDRWSEARRLFDSLAVDDEQTCATIAQVHAQTGYLLDPHTAIGVK
                     AARDCRRSLALPMVVLGTAHPVKFPEAVEKAGIAAVPQLPPHLADLFQREERCTVLAN
                     DLKTVQDFVSQHGNRGKPL"
     gene            19851..21329
                     /gene="rmuC"
                     /locus_tag="VIO83_06840"
     CDS             19851..21329
                     /gene="rmuC"
                     /locus_tag="VIO83_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY8331024.1"
                     /translation="MPFELSSLLLGLVAAALPAAALLWQLQQQRSLAQQQLGQLAERL
                     TASEQAREELAAQLGQARSEAQGQQRQLLTLQGETAGLRRETQLLTVERQRAAEAAQQ
                     WQQERQQREEELRDLAAERAALAAALREQQQGHRQRLEEVQSARDELRAQFAELAGKI
                     FDEREQRFAESSQQRLGQLLDPLRERIHSFEQRVEQSYQQEARERFSLGKELERLQQL
                     NQRLGDEATNLTRALKGQKTQGNWGELVLERVLEHAGLEKGREYDTQVSLRGAGGERY
                     QPDVLIRLPGERQVVVDAKVSLTAYQQYVGAEDDGLRQQALKQHVQSLRSHLKGLSGK
                     DYQRLEGLHSLDFVLLFVPLEGAFAAALQAEPGLFEESFAQNIVIVSPTTLLATLRVI
                     DSLWRQERQDLNAREIAERAGALYDKFVLFVQDLDEIGNRLQQLDKAYANARNKLTAG
                     RGDLISRSEQLKLLGARASKSLPADWLARSASLPLALDEDPA"
     gene            complement(21413..22669)
                     /locus_tag="VIO83_06845"
     CDS             complement(21413..22669)
                     /locus_tag="VIO83_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEY8331025.1"
                     /translation="MTPDLDTWLNLASALAIGLLIGAERGWSAREILDTRLSAGIRTF
                     GLVGLLGGLGTLLAGHFGPLAWLGLLLAVAVLVTTAYLIDQKHDDDPGLTSEMALLLT
                     FLLGSLALAHSRLLAGGCAVVVALLLSLKEPMHSALKRLSAAELSGALKLLFISLVLL
                     PALPDQGYGPWQVFNPYATWWMVVLIAGLGFAAYAAMRLVGTRHGLLLTALLGGVVSS
                     TAMTLTLSRLHSQRELHALLAAGLLTTSALMFPRVLLEVAAINPHLLAELLWPLGAAA
                     LVYAGGALYWWRRAANSEQAPSAEPPLKNPFELGPALRFAVLLAVILLLVEVGRRELG
                     DVGVYLVAILSGLTDVDAITLSLSRSALHELDAQVAVRGIALAVISNSLIKGVLIAIV
                     GGPRLALLTVPVMAGGLGLGLALVLL"
     gene            complement(22754..26236)
                     /locus_tag="VIO83_06850"
     CDS             complement(22754..26236)
                     /locus_tag="VIO83_06850"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HEY8331026.1"
                     /translation="MSLSSGLITAVALSYMAVLFAIAFYGDRRQTSPRPQRPRLRAWV
                     YSLSLAVYCSSWTFFGSVGQAAGQLWSFLPIYLGPLLVLLVMPQMLAKMILISKQENI
                     TSIADFISARYGKSRPLAIVVALICLVGVLPYIALQLKGIVLGVNLLTGARADANGIS
                     AQDTALIVSLILALFTILFGTRNLDATEHHRGMVMAVAFESLVKLLAFLAVGVFVTWG
                     LFDGFDDLLAKAQAAPKLEDYWQQPLDWPAMLVQTGVAMMAIICLPRQFHVTVVENIE
                     PRDLNVARWVFPLYLALAALFVIPIALAGQLLLPAGVEPDSFVISLPLAEAHPALALL
                     AFIGGASAATGMVIVASVALSTMISNDMLLPWLLRRHSAERPFEEFRHWLLSVRRIAI
                     IALLLLAYVIYRMMGSNISLAGIGQIAFAAITQLGPAMFGALYWRQANSRGVFAGLAV
                     GALLWCYTLLLPLVAAGMGWSLDLFPGQRWLAGNPLGIPMDALTQGALLSLTGNFVVF
                     ALVSWLSRTRVSEHWQASRFVGQEASVRPSPRLLLAVQVQDLLTLAGRFVGEERARQS
                     FIRFAYRQGQGFSPNQTASSEWIAHTERLLAGVVGASSARAVVKATIEGRDMQVEDVV
                     RIVDEASEVLQFNRALLQGAIENITQGISVVDQSLRLVAWNRRYLELFEYPEGLISVG
                     RPISDIIRYNAERGLCGRGDADLHVAKRLYWMRQGTAHTSERIFPNGRVIELIGNPMP
                     GGGFVMSFSDITAHRQAEDALKEANESLEQRVAERTRELSELNEQLSEAKARAESANQ
                     SKSRFLAAVSHDLMQPLNAARLFSAALAHQEALPVEVQELVHHLDSSLRSAEDLIADL
                     LDISRLEGGRFTPERSTFALSTLYDALGVEFRALAQEQGIDLRIRASKLSIDSDSKLL
                     RRVLQNFLTNAFRYAKGKVLLGMRRDGQHLRLEVWDRGPGIPADKLEVIFEEFKRLDS
                     HQTRAEKGLGLGLAIADGLCRVLEHKLEVRSWPGKGSVFSVRVPLACGRAQQAPQRLQ
                     TTAGQAMNGAQVWCIDNEDSILTGMRSLLSRWGCQVQTARTREECAALLDGGSTPELV
                     LVDYHLDGGETGLALMGWLRARLGQPVPGVVISADGRAELIAEVHAGGLDYLSKPVKP
                     AALRALISRHLNLR"
     gene            complement(26457..27197)
                     /locus_tag="VIO83_06855"
     CDS             complement(26457..27197)
                     /locus_tag="VIO83_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HEY8331027.1"
                     /translation="MFQYSARPDLLKDRVILVTGAGRGIGAAAARTFAAHGATVILLG
                     KTIEHLEEVYDQIEAAGGPQPAIFPFNLETAQQHQYDDLAATIEREFGHLDGLLHNAA
                     LLGPRTPIEQFSGDNFMRVMQVNVNAVFMLTSVLLPLLKLSNDASVVFTSSSVGRKGR
                     AYWGAYAVSKFATEGIMQVLADEVDGISAVRANSVNPGATRTSMRAQAYPGENPQQNP
                     APEEIMPVYLYLMGPDSTGVNGQAFDAQ"
     gene            complement(27402..28073)
                     /gene="mupP"
                     /locus_tag="VIO83_06860"
     CDS             complement(27402..28073)
                     /gene="mupP"
                     /locus_tag="VIO83_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017906402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate phosphatase
                     MupP"
                     /protein_id="HEY8331028.1"
                     /translation="MRLRAVLFDMDGTLVDSAPDFIAVCQAMRAARELPPLDPQRIRD
                     QVSGGARAMVACTFGLTPEHPEFEPLRQEFLARYQQHCAVLTQPFDGIPELLADLEQA
                     RLRWGVATNKPLHYAEPIMQHLQLAERAAVLVCPEHVSRSKPDPEMLLLACQQLDLDP
                     REVLYVGDDERDIEAGRAAGTRTVAVRYGYIHPDDNPGHWGADLVIDHPRELRDVLER
                     ALCGC"
     gene            complement(28079..28777)
                     /gene="ubiG"
                     /locus_tag="VIO83_06865"
     CDS             complement(28079..28777)
                     /gene="ubiG"
                     /locus_tag="VIO83_06865"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003172645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEY8331029.1"
                     /translation="MSNVDHAEIAKFEALAHRWWDRESEFKPLHEINPLRVNWIDERV
                     GLAGKRVLDVGCGGGILSESMALRGATVTAIDMGEAPLAVARLHQLESGVQVDYRQST
                     AEALAAEMPGQFDVVTCLEMLEHVPDPASVIAACQRLVKPGGQVFFSTINRNPKAYLL
                     AIVGAEYLLKMLPRGTHDFKKFIRPSELGAWCRSSGLEVKDIVGLTYNPLSKTYKLGS
                     DVDVNYMIQTLREE"
     gene            complement(29002..30327)
                     /locus_tag="VIO83_06870"
     CDS             complement(29002..30327)
                     /locus_tag="VIO83_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HEY8331030.1"
                     /translation="MPSTTAPLDLLLLPEWIVPVEPAGVVLRDHALGIRDGRIALLAP
                     RQQALAIPARERRELPGMLLAPGLVNAHGHAAMSLFRGLADDLPLMTWLQEHIWPAEA
                     KWVSADFVRDGSELAIAEQIKGGISAFSDMYFFPEVASEVVHASGVRAQITIPVLNFP
                     VPGAHDTDEALRRGLELFADLRHHPRIKVAFGPHAPYTVSDDRLERVRVLAEQLDAGI
                     HMHVHETAHEVQQALELSGERPLARLARLGLLGPRFQAVHMTQIDDSDLALLVQHNSS
                     VIHCPESNLKLASGFCPVERLWQAGVNVAIGTDGAASNNDLDLLGETRTAALLAKAVA
                     GSATALDAHRALRMATLNGARALGIDDECGSLEAGKSADLVAFDLSGLAQQPVYDPVS
                     QLIYASGRDCVKHLWVGGKALLEDGRLTRLDEDELRAKARAWGQRIAGV"
     gene            30460..31536
                     /gene="mtnA"
                     /locus_tag="VIO83_06875"
     CDS             30460..31536
                     /gene="mtnA"
                     /locus_tag="VIO83_06875"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEY8331031.1"
                     /translation="MREQLLAAEKVTAIDWRHGVLHLLDQRALPLEETWLAYDSAAGV
                     AEAIRLMVVRGAPAIGIAAAYGVVLGVRARLAAGGDWQAALEEDFRVLAESRPTAVNL
                     FWALDRMRERLNRLADCDDVLAALEAEAVGIHASDREANLTMAQLGVELIRRHNSHPQ
                     KLLTHCNTGALATGGFGTALGVIRAAHLAGLVERVFADETRPWLQGARLTAWELAGEG
                     IPVSLNADAAAAHLMKTEGIAWVIVGADRITANGDVANKIGTYQLAVNAMHHGVRFMV
                     VAPSSTIDMNLESGDDIPIEERDGRELLEIGGQRVAADVHAVNPVFDVTPADLIDAIV
                     TEKGVVERPDAEKMKQLMCRKRLH"
     gene            31722..34376
                     /gene="gyrA"
                     /locus_tag="VIO83_06880"
     CDS             31722..34376
                     /gene="gyrA"
                     /locus_tag="VIO83_06880"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007963619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY8331032.1"
                     /translation="MGELAKEIIPVNIEDELKQSYLDYAMSVIVGRALPDARDGLKPV
                     HRRVLFAMSELGNDWNKPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSVDGDSAAAMRYTEVRMAKLAHELLADLDKETVDWVPNYDGTEQIPAVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLSEVIDGCLALIDNGELTVDELMQYIPGPDFP
                     TAAIINGRAGIIEAYRTGRGRIYMRARSMIEDIDKVGGRQQIVITELPYQLNKARLIE
                     KIAELVKEKKLEGITELRDESDKDGMRVVIELRRGEVPEVILNNLYAQTQLQAVFGIN
                     IVALIDGRPRILNLKDLLEAFVRHRREVVTRRTVFELRKARERGHILEGQAVALSNID
                     PVIALIKASPTPSEAKEALVSTPWESSAVVAMVERAGADSCRPENLDPQYGLRDGKYF
                     LSPEQAQAILELRLHRLTGLEHEKLLAEYQEILNQIGELIRILNSATRLMEVIREELE
                     VIRAEYGDVRRTEILDARLDLTLGDMIPEEDRVVTISHGGYAKTQPLAAYQAQRRGGK
                     GKSATGVKDEDYIAHLLVANSHTTLLLFSSKGKVYWLKTYEIPEASRAARGRPLVNLL
                     PLDDGEYITTMLPVEEYTEGHFIFMATANGTVKKTPLESFSRQRSVGLIALELDEGDV
                     LISAAITDGEREVMLFSDGGKVTRFKESDVRAMGRTARGVRGMRLPEGQKLISMLIPE
                     EGSQILTASARGYGKRTAITEFPEYKRGGQGVIAMVSNERNGRLVGAVQVLDGEEIML
                     ISDQGTLVRTRVDEVSSLGRNTQGVTLIKLANDETLVGLERVQEPSEVEGEELEGEEG
                     AVFEGTAVIDDIVEDQQLDAAADEEPQE"
     gene            34627..35712
                     /gene="serC"
                     /locus_tag="VIO83_06885"
     CDS             34627..35712
                     /gene="serC"
                     /locus_tag="VIO83_06885"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEY8331033.1"
                     /translation="MSKRAYNFCAGPAALPEAVLQRAQGELLDWHGKGLSVMEMSHRS
                     DEFVSIATKAEQDLRDLLNIPSNYKVLFLQGGASQQFAQIPLNLLPESGTADYIDTGI
                     WSQKAIEEASRFGNVNVAATAKPYDYFAIPGQNEWQLSKDAAYVHYAPNETIGGLEFQ
                     WVPETGDVPLVADMSSDILSRPIDVSRFGMIYAGAQKNIGPSGIVVNIVREDLLGRAR
                     SICPTMLDYKVAADNGSMYNTPPTLAWYLSGLVFEWLKEQGGVEAIGKLNDVKQRTLY
                     DFIDASGLYSNPINKSDRSWMNVPFRLADDRLDKPFLAGADERGLLNLKGHRSVGGMR
                     ASIYNAVDINAINALVAYMAEFEKEHG"
     gene            35712..36806
                     /gene="pheA"
                     /locus_tag="VIO83_06890"
     CDS             35712..36806
                     /gene="pheA"
                     /locus_tag="VIO83_06890"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY8331034.1"
                     /translation="MSEQELKALRLRIDALDEKVLELISERARCAQEVARVKMASLAE
                     GEVPVFYRPEREAQVLKRVMERNQGPLGNEEMARLFREIMSSCLALEQPLKVAYLGPE
                     GTFTQAAAMKHFGHAVISKPMAAIDEVFREVAAGAVNFGVVPVENSTEGAVNHTLDSF
                     LEHDMVICGEVELRIHHHLLVGENTKTDSISRIYSHAQSLAQCRKWLDAHYPNVERVA
                     VSSNAEAAKRVKGEWNSAAIAGDMAAGLYGLTRLAEKIEDRPDNSTRFLMIGNQEVPP
                     TGDDKTSIIVSMSNKPGALHELLVPFHDNGIDLTRIETRPSRSGKWTYVFFIDFVGHH
                     RDPLVKGVLEKISQEAVALKVLGSYPKAVL"
     gene            36820..>36914
                     /locus_tag="VIO83_06895"
     CDS             36820..>36914
                     /locus_tag="VIO83_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331035.1"
                     /translation="MSGNFLALAQPGVQQLSPYVPGKPVDELAREL"
BASE COUNT         6416 a        11986 c        12562 g         5950 t
ORIGIN      
        1 aggggcagga gtcgtggagc gaggaggaca tccgcgcttc ccgccagctg ctgcgcgagg
       61 ccacgacggc gcaggcgccg gtctaccaga ccgatccgct ggggctgatg ctggtggtcg
      121 agactggcga ttctcacatc acgatccttg atggcgacaa gctcgagccc tttcatcgtt
      181 tcgccacccg ggcgcagctg catggcagcc cgaaattcac ccgcgacggt cgctacgcat
      241 tcttcgtctc ccgcgatggc tgggtgacca agtacgacct gtggacgctg gaggtcgtgg
      301 ccgaggtgcg ggcggggctg gacacccgca acattgccgt cgacccgacg ggcgatcacg
      361 tcgctgtggc caactaccag ccccataccc tggtgctgct ggacggcgac ctgaacctgc
      421 tcaaatcgat gccagctctc gaccagaagg cggagcacag ctcgcgggtg tcggcggtct
      481 acagcgtgcc gtcgcgacgg tcgttcgtcg ttgcgctgaa ggacatcgcg gagttgtggg
      541 aggtcagcta cgacccgcag gcagaagaca tggcgatcgg tctggtgcac gacttccagt
      601 atcgggaggg caccttcgtc acggggtatc tcaatccacg acgcatcgag ctggacgcgc
      661 cgctggatga tttcttcttc aatcctgatc aggccgagct gttcggcgtg tctccgcagg
      721 gcgaggtgca ggtgatccag ctggatgcgc gccagcgaat cggcggcttg cctttgcagg
      781 ggcgcccgca catgggcgcg gccgtcacct ggagccgcca gggggtgacg ctgatggcca
      841 gcaccaacct caagctgccg gaagttgcgg tggtcgaccc gcagcggcgc gaactggtgc
      901 agcgcatcgc cctgcaaggt cccgggtttt tcctcgccgg ccatgagaac agccgctact
      961 tgttcgtcga ttcgatgctg gcggccgatt cgcggcatgt gctgcaggcg atcgaccggg
     1021 aaaccctgga ggtcgcgcgg cgcttcgcgg ccgagccggg cgatacgctg gcgcacgtcg
     1081 aattcacccg cgacggccgc tacgcgctgg ccagcctgtg gcgcgacgac ggtgccgtga
     1141 tcgtgttcga tacgcagagt ctggaggagg tcaaacgcct gccgatgaag cggccggtgg
     1201 gcaaatacag cgtctggcgt ggcatcgagt cccagcaggg cgctgcgcgt tagcgtcggt
     1261 cgggctcggg cgctcgtcgg cggctgcgct tgcagcgggt cgctgcccct ttgaccgccg
     1321 tcatgggcta gaatacgcgc cccgactgct cccctcttcg aggctgttcc gacgatgttg
     1381 attctgcgcg gcgctcccgc tctttccgcc ttccgtcacg gcaagctcct ggcacaactg
     1441 accgagaagg tccccgccgt gagcgggctg tatgccgagt ttgcccattt cgccgaggtt
     1501 tccggcgcgc ttggcgcgga agagcagcag gtcctgaccc gcctgctcaa gtacggcccc
     1561 agcgtgccga tgcaggagcc agccggtcgg ctgttcctgg tggtgccgcg tttcggcacc
     1621 atctcgccct ggtcgagcaa ggccagcgac atcgcccaca actgcagcct ggagaagatt
     1681 cagcgcctgg agcgtggcat cgcctactac gtgcagggcg agctgtccga cagtgacgcc
     1741 gagctcgtcg ccgccgcgct gcatgaccgc atgacccagc tggtgctcgg ccgtttcgag
     1801 gaagccgcca acctgttcag ccatgcccag cccaagccgc tcactgccgt ggatgtgctc
     1861 ggcggcggtc gcgccgcgct ggaaaaggcc aacgtcgagc tgggcctggc gctggccgaa
     1921 gacgagatcg actatctggt gcaggccttc accgggctgg ggcgcaaccc gcacgacatc
     1981 gaactgatga tgttcgccca ggccaactcc gagcactgcc gccacaagat cttcaatgcc
     2041 agctgggaca tcgacggcga aagccaggac aagtccctgt tcggcatgat caagaacacc
     2101 taccagctgc acaacgaggg tgtgctgtcg gcctacaaag acaacgccgc cgtcatcgtc
     2161 ggcaatgttg ccggccgctt ctatccgaat ccggagacgc gcgagtacgc cgcgagccag
     2221 gagccggtgc acatcctgat gaaggtggag acgcacaacc acccgaccgc catcgcgccg
     2281 ttcccgggtg cctccaccgg ctccggcggc gaaatccgcg acgagggtgc gaccggtcgc
     2341 ggtgccaagc cgaaggccgg cctgacgggc ttcaccgtct ccaacctgaa catccctggc
     2401 ttcgagcagc cctgggagaa gccctatggc aagcccgagc gcattgtcac gccgctggac
     2461 atcatgatcg aaggcccgct gggcggcgcc gcgttcaaca acgaattcgg ccgcccggcg
     2521 ctgactggct atttccgcac cttcgagcag gcgatcgaca cgccgcgcgg ggaggaagtg
     2581 cgcggctatc acaagccgat catgctcgcc ggcggcatgg gcaatatccg tgaagaccac
     2641 gtgcagaagg gcgagatctc ggtcggcggc aagctgatcg tgctcggcgg cccggccatg
     2701 ctgatcggtc tgggtggcgg cgcggcctcg tcgatggcca ccggcaccag ctcggcggac
     2761 ctcgacttcg cctcggtgca gcgcgacaac ccggaaatgg agcgccgctg ccaggaagtt
     2821 atcgaccgct gctggcagct gggcgagcgc aacccgatca agttcattca cgacgtcggc
     2881 gccggcggcc tgtccaacgc cttccctgag ctggtcaacg atggcggtca cggtggtcgc
     2941 ttcgagctac gcaacatccc caacgacgaa cccggcatgg cgccgcacga gatctggtgc
     3001 aacgaatccc aggagcgcta cgtgctctcg gtcgatgccg ccgatctgga gcgcttcgcc
     3061 gccatctgcg agcgcgagcg ctgccccttt gcggtcgtcg gtgaggccac cgaggaagcg
     3121 cacctgaccg tcgccgacag ccatttcggc aacaagccgg tggacatgcc gctcaacgtg
     3181 ttgctcggca aggcgccgcg catgcatcgc agcgcgcagc gcgaagccga actgggcgac
     3241 gatttcaatg ccgcgtcggt ctacctcgac gaggctgtgg gccgcgtgct gcgccacccg
     3301 gcggtggcga gcaagagctt cctgatcacc atcggcgacc gcagcatcac tggcctcgtg
     3361 gcgcgcgatc agatggtcgg accctggcaa gtgccggtgg ccgattgcgc ggtgaccgct
     3421 gccagctatg acgtctatac cggcgaggcg atggcgatgg gtgagcgcac gccgctggcc
     3481 ctgctcgacg cgccggcctc cggccgcatg gcgatcggcg aaacgctgac caacctcgcc
     3541 gcggcacgca tcgagaaact ctccgatatc aaactgtcgg ccaactggat ggccgctgcc
     3601 ggccacccgg gcgaggacgc ccgcctgtac gacacggtca aggcggtggg catggagctg
     3661 tgcccggcgc tgggcatcac cattccggtg ggcaaggact ccatgtcgat gaagacccgc
     3721 tggcaggacg cgggcgccga caagagcgtc accgcgccga tgtcgctgat cgtctccggc
     3781 ttcgccccgg tgcaggacat ccgccgtacc ctgaccccgc agctgcgtct ggaccagggc
     3841 gccagcgagc tgctgctgat cgatctcggc cgtggccaga accgcctggg cggctcgatc
     3901 ctggcccagg tctacagcca gatcggtcgc agcgtgccgg acgtcgacaa ggccgaagac
     3961 ctcaagggct tcttcgacac catccagggc ctgaacgccg acggcaagct gctggcctac
     4021 cacgaccgtt ccgacggcgg cctgctgacc accgtggtgg aaatggcctt cgccggccat
     4081 tgcggtctcg atctgcagct cgacgtgctg gctgccagcc gcgcggcgct cggcgccgcg
     4141 ctgttcagcg aggagctggg cgcggtggtc caggtccttc agaccgacac cgccgaggtg
     4201 ctggcgcgct tcgccgccgt tggcctggcc gactgcgtca gcgtgatcgg ccagccggtc
     4261 gccggcggcg acatccgtct gggtctgggt ggcgacgtga tctacagcgc cgagcgccgc
     4321 atcctgcagc gcatctggag cgagaccagc taccgcatcc agcgtatgcg cgacaacgcc
     4381 cagtgcgccg agcaggagtt cgacctgctg ctcgacgaga gcaaccccgg cctgtcggtc
     4441 aagctcggct tcgatgtcaa cgacaacgtc gctgcgccct atatcaacac cggtgtgcgt
     4501 ccgcgcatgg cgatcctgcg cgagcagggc gtcaacggcc agaccgagat ggcggccgct
     4561 ttccaccgcg ccggcttcgc ggtggtcgac gtgcacatga gcgacatcat cgccggtcgc
     4621 gtcagcctgg aagacttcaa gggcctggtg gcctgcggcg gcttctccta cggcgacgtg
     4681 cttggcgccg gtggcggctg ggccaagtcg gtgctgttca acaatcgcgc ccgcgacaac
     4741 ttcagcgcct tcttcgagcg caaggacagc ttcgccctcg gcgtgtgcaa cggctgccag
     4801 atgctctcca acctgggcga gctgattccg ggcagcgagg cctggccgcg cttcgtgcgc
     4861 aaccgctccg agcagttcga ggcgcgggtg gccatggtcc aggtgcagga gtcggcgtcg
     4921 atcttcctgc agggcatggc cggttcgcgt ctgccgatcg ccatcgccca tggcgagggg
     4981 catgccgagt acgccagcga ggaggccctg ctgcaggccg acctgtccgg cgccgtggcg
     5041 ctgcgcttcg tcgacaacca tggcaaggtc accgagagct acccggccaa ccccaacggt
     5101 tcgccgcgcg gcatcaccgg cctgaccacc cgcgacggtc gcgtcaccat catgatgccg
     5161 cacccggagc gcgtgttccg cgccgtgcag aactcctggc gtccggacga ctggcaggaa
     5221 gatgccggct ggatgcgcat gttccgcaac gcgcgggtct gggtcgactg agcccatgcc
     5281 ttcccgccgg tggccttcgg gctgccggtg ggcggcaagt tttattgcag tggcgcctgt
     5341 ttggcgccat ttctttgtgt ggggcgtgat cttgttcgcg gatttggcgt ctgaggatgg
     5401 attacgtttt tgtgctgtca gggacgaagc acctggtcct gagcgaccga attatcaccc
     5461 agtatcttct gtggccaagt gccactgccg ccgcaactct cttcgccccg gagttcttga
     5521 tgtacaagct gtgtttcttc gttcccgagt cccatctgga aacggtcaag accgcgatct
     5581 tcgctaccgg tgccggtcgc atcggcagtt acgacagctg ctgctggcag acccttgggc
     5641 agggacagtt ccatccgctg ccgggcagcc agccctacga gggtgcccag ggtgagctgg
     5701 cgcaggtggc cgagtggaag gtcgagatgg tggtggccga cgagttgatc catgcggcgg
     5761 tcaaggcgct caagcagaat catccctacg agacgccggc tttcgacgtc tggcgcctgt
     5821 cggacatggt gttctaggcg gtacggggga gccgcaacgc gtgctcgcca caaacaaaaa
     5881 aggtgccgac atcgacgatg cggcaccttt tttgtttgac cgtccacagc gtcaggggcg
     5941 gatctcgatc agcgtgccgt ccttgaccag gctccacacc tcgcgaatgt cgtggttgcg
     6001 catggcgatg cagccctcgg tccagtcgag ggtgtggaag taccactcgg gatattcctc
     6061 atccagcggc gtgccgtgga tcatgatcat gtcgccggcc ggcaggccct tctgccgggc
     6121 gtggtgcaga tcctgctggt tggggtagga gatgtgcatg gacaggttgt acttgtcgct
     6181 ggccttgcgc cagtcgatcc agtagaagcc ttccggggtg cgcttgtcgc cctggtagcg
     6241 cttcgggcca ttgggttgct tgcccagcga gacccggtag ctcttgagga cttcgccgcg
     6301 gctgagcagg agcagcttgc gctcggactt gaccaccagc accttgtcga ctgccggcct
     6361 gtcgaggcct ggcgtgctca tggcattagc cagggtggcg aacagaaaac agcagaagac
     6421 aatcgaccag cgcatgacag gcattccagt gcgaaccagg gtggccgtca gcccggcggc
     6481 cggttattca tgccgcgcgc tgctggggct gatcagcgcg cagttacgcc gtcctcgtgc
     6541 cggcgtttcg ctgtcaattc taggggagcg ccacgtggag ggaaagcgcg atatgccggt
     6601 tgccggcgga gggcttgggc gcgtccgggc tttcatgcca tgattcaagg cgaacaaatg
     6661 acgagactgc cccatgctgg acgaactgcg ccaacgcgtg caggcccata gccccggatc
     6721 cctgcccagc gatcgcaact tccccgaagc ggcggtgctg atgccgatca cccgcagcga
     6781 cgacccgcag ctggtgctga ccctgcgtgc cagcggcttg tccacccatg gtggcgaggt
     6841 ggcctttcct ggcgggcggc gcgatcccga ggacgagtcg ctggtcttca ccgccctgcg
     6901 cgaggcgtac gaggaggtgg ggctggcgcc cggtctggtc gaggtggtcg gtccgctcag
     6961 cacgcagatc tcccgccacg gcatcaaggt caccccctat gtcggcttta tccccgacta
     7021 tgtcgagttc aaggccaacg acgacgagat tgccgcggta ttctcggtgc cgctggagtt
     7081 tttccgcgac gacccgcgcg agatcaccca tcgcatcgac tacctcggct acagctggta
     7141 cgtgcccagc taccgctacg gcggcttcaa gatctggggc ctgaccgcga tgatggtggt
     7201 cgagctggtc aacctgatct acgacgccgg catcgagttg cgcgagccgc ccccccactt
     7261 cagtcactat cgttgagcga tccgaggaac ctgccgatga aataccatct gggcgatgcc
     7321 cgtgtcgaag cccatcccga cagctggatt gcgcccactg ccagcgtgat cggcaaggtg
     7381 cgtctggagg ccggcgccag tgtgtggttt ggcgccgtgc tgcgcggcga caacgagctg
     7441 atccacgtcg gcgagaacag caacgtgcag gacggcagcg tgctgcacac cgacatgggc
     7501 tggccgttga ccatcggcaa gggcgtcacc atcggccaca aggtcaacct gcatggctgc
     7561 agcatcggcg accacagcct gatcggcatc aatgccgtgg tgctcaacgg cgcgaagatc
     7621 ggccggcact gcctgatcgg cgccggtgcg ctgatccccg agggcaagga aattcccgac
     7681 ggcagcttgg tgatgggcgt gccgggcaag gtggtgcgca gcctcagcga ggaacagatg
     7741 cgcggcctgg aaaacaacgc cgcgcactac gtcgacaatg cccggcgcta tgcccgcgaa
     7801 ctggccgagc aggccgactg atggccgagc ggcaacagga caacgtgcag gaaaaaccgc
     7861 tcgcctcgcc ctgcgtgtac gtctgcgccc tggatgacga cgacatctgc atcggctgcc
     7921 agcgcagcgg tgaggaaatc acccgctggg ggcggatgga caacgccgag cgccgcgccg
     7981 tgctcgtgcg ctgtcaggag cgcgccctgg cggccggaca gtttctggta aaacccaatc
     8041 ccctctgaag gaagcattcc gtgaccatga tcggaacccc gctgtcgccg agcgcgaccc
     8101 gtgtgctcct gtgcggctct ggcgagctgg gcaaggaagt cgccatcgag ctgcaacgcc
     8161 tgggctgcga ggtgatcgcc gtcgaccgct acgccaacgc cccggccatg caggtggcgc
     8221 atcgcagcca cgtgatcagc atgcttgacg gcgccgcgct gcgtgcagtg atcgagcagg
     8281 agcagccgca ctacatcgtt cccgagatcg aggcgatcgc caccgacacc ctggtcgagc
     8341 tggaagccga gggcttcacc gtggtgccca cagcgcgcgc cgcccggctg accatgaacc
     8401 gcgagggcat ccgccgcctg gcggccgagg agctgggcct gccgacctcg ccgtaccgct
     8461 tcgccgactc cttcgccgac tacgccgcgg cggtggccga gctgggcttc ccctgcgtgg
     8521 tcaagccgat catgagctcg tcgggcaagg gccagagcct gctgcgcggc gaagccgatg
     8581 tgcagcaggc ctgggactat gcccaggaag gcggccgcgc cggcaaggga cgggtgatcg
     8641 tcgagggctt catcgacttc gactacgaaa tcaccctgct caccgtgcgc catgccggcg
     8701 gcaccacctt ctgtgcgccg gtcggtcatc gccaggagaa gggcgactac caggagtcct
     8761 ggcagccgca ggcgatgagt cccaaggcgc tggccgaatc cgagcgcatc gcccatgcgg
     8821 tgaccgagtc cctcggcggg cgcggcctgt tcggcgtcga gctgttcatc aagggcgagc
     8881 aggtgtggtt cagcgaggtg tcgccgcgcc cgcacgatac cggcctggtc accctgattt
     8941 cccaggatct gtccgagttc gccctgcacg cgcgggccat cctcggcctg ccgatcccgg
     9001 cgatccgcca gttcgggccc tcggcctcgg cggtgatcct ggtcgaaggc gagtcgaagg
     9061 aagtgcgttt cggcaacctg ggtgcggcgt tggccgaggc ggacaccgcc ctgcgcctgt
     9121 tcggcaagcc ggaggtcagc ggccagcggc gcatgggcgt ggcgctggcg cgcgacgcgt
     9181 cgatcgagac ggcgcgcgcc aaggcgctgc gtgcggcgca ggcggtgacg atccagctgt
     9241 aagacggatc tgcccgtaaa aaagggccgg ccctcgcgag aggtccggcc cttttcgttg
     9301 cggcggggtg gggtcaggcg gccggccccg gcaggcgttg gccgtgcggc cagatgcgta
     9361 cctgggtcac gcggttttcc gcggactgca ggatctccag gcggtaggcg ccgatcttca
     9421 ggcacacgct gctgtcgggc aggttttcca gggcttcggt gaccaggcca ttgatggtcc
     9481 gggggccgtc gcagggcagg cgccagctca gctcgcggtt gacctcgcgg atgtgggcgc
     9541 tgccgtcgat cacgtagctg ccgtcgtcct gggggtggat ttccggttca tgcaccccgg
     9601 gctgggtggt gaattcgccg acgatctcct cgaggatgtc ctccagagtg accaggccga
     9661 tgacctcgcc gtactcgtcg accaccatgc ccatgcgccg cttgtgcttc tggaagttga
     9721 tcagctgggt cgacagcggg gtgttttccg gcacgaaata gggagtcacg caggcgctgc
     9781 ggagcagttc gtggctcggc cggttgctgg tcagcaggcg ggcgatgcgg cgcatgtgca
     9841 ccacgccctc gacctggttg aggtcgtggc ggtagaccgg caggcgcgtg tgggtggtgc
     9901 tgcgcagttg cgcggtgatc tgctcgaggt cgtcctccag gtcgatgccg accacctcgt
     9961 tgcgcgggat catgatgtcg ttgaccgtga cccggtccag ctcgaggatg ccggccagca
    10021 tggcctggcg gctgcggggc tggccgggtg cggactcgtg cacggcgtcg cgcagttctt
    10081 cgtgatcggt cagctcggcg gcggggggcg gcagcgggcg cagcagttgc cggagcctgc
    10141 gcagcaggcg gggcaggagc ccggtatggt cgtcgcccac ggcgtctgcc ctcagatatg
    10201 caggatgaat tcgcggacca gcttgctgcc gaagtaggcc agcatcagca gcaggaagcc
    10261 ggccagggtc cagcggatcg cctggccgcc acgccagccg agctggtggc ggccccacag
    10321 cagcacgccg aacaccaccc aggccacgca ggcgagcagg gtcttgtgca ccaggtgctg
    10381 ggcgaacagg tcgtcgagga acagccagcc ggacagcagc gacagcgaca gcagcgacca
    10441 gccgccccac aggaagccga acagcaggct ttccatggtc tgcagcggcg ggaagttgcg
    10501 gatgatgccg gacgggtgct tgttcttcag ctggcggttc tgcagcagca gcagcagggc
    10561 ctggaccacc gcgatggtga gcaggccgta ggccaggatc gacagcagga tgtgggcgag
    10621 gatgcccggc tcctcgacga tcggctgggt ggtgccgcct ggtagcaggg cgagcagggt
    10681 ggtcaggctg cccagcggga acagcaggac cagcaggttt tccaccggga tgcgcaggct
    10741 ggccagcagg gtcagggcga tgacggcggc ggccagcagg ctggaggcat tgaagaagtc
    10801 gagctgcagt gcgccgttgt tcagcagcag gcgcaagccg aaggcgtggg ccagcagggc
    10861 gagcacgcca aggagaccga gcaggcgctt gtcaggcgtc gcacgacggg acagcagcag
    10921 tgcctgatag agtgcagcgc cggcatacag gccggccgcc gccaggctgg gcagcaaagg
    10981 cgtcataagt ccttgtggag gaagcctcgg agttgccgag tttggcacac aaccgactat
    11041 ccacgaaagt ctgcaggcgg tgtcggcggc ggccggtctc ggctataatc cgccgcctgc
    11101 cgcaagctcc atcttccaag gaacgtgcat gttcgagaat ttgaccgacc gcctctcaca
    11161 gacgctgcgc aatgtcaccg ggcgcgccaa gctgaccgag gacaacatca aggacaccct
    11221 gcgcgaggtg cgcatggccc tgctcgaggc cgatgtcgcc ctgccggtgg tcaaggactt
    11281 cgtcaacaag gtcaaggatc gcgcggtcgg caccgaggtg tcgaagagcc tgacgccggg
    11341 ccaggcgttc gtgaagatcg tccgcgccga gctggaagag ctgatgggcg cggccaacga
    11401 ggacctgaac ctcgctgcca cgccgccggc ggtgatcctg atggccggcc tgcagggcgc
    11461 gggcaagacc acgacggtcg gcaagctggc gcgcttcctc aaggagcgca agaagaagtc
    11521 ggtgctggtg gtgtcggccg acgtctaccg tccggcggcg atcaagcagc tggaaacctt
    11581 ggccaatgag gtgggggtga ccttcttccc ctccgacctc agccagaagc cggtggacat
    11641 cgcccgcgcg gccatcgccg agggcaagct gaagttcatc gacgtggtga tcgtcgacac
    11701 cgccggccgt ctgcacatcg acgccgagat gatggacgag atcaagcagg tccatgcggc
    11761 gatcaacccg gtggagaccc tgttcgtggt cgacgccatg accggccagg atgccgccaa
    11821 caccgccaag gccttcaacg aggccctgcc gctgactggc gtggtgctga ccaaggtcga
    11881 cggcgatgcc cgtggcggcg ccgcgctgtc ggtacgcgcc atcaccggca agccgatcaa
    11941 gttcctcggc atgggcgaga agagcgaagc gctcgatccg ttccatcccg accgcgtggc
    12001 ctcgcgcatc ctcggcatgg gtgacgtgct cagcctgatc gagcaggccg agcagaccat
    12061 ggaccgcgag aaggcggaga agctcaccaa gaagctgaag aagggcaagg gcttcgatct
    12121 cgaagacttc cgcgaccagc tgcaacagat gaagaacatg ggcggcctcg gcagcctgat
    12181 ggacaagctg ccgatgctcg gcggcatgaa cctcggccag atgggcaacg cccagaacgt
    12241 ggcggagaag cagttcaagc agatggaggc gatcatcaac tcgatgaccc ccctcgagcg
    12301 ccgcgatccg gaagtcatca gcggctcgcg caagcgccgc atcgccctgg gttccggcac
    12361 ccaggtgcag gatgtcggtc gtctgatcaa gcagcacaag cagatgcaga agatgatgaa
    12421 gaaggtcacc gccaagggcg gcatggccaa gatgatgcgc ggcatgagcg gtctgctgcc
    12481 cggcggcggc atgccgaaga tgtgagaatc gggcggcgac gcttgtcgtc gtcgccgatc
    12541 atcgcgggcc gctccggcgg tgcgcgacaa acagaggatg actccgtctt ccagtgcccg
    12601 ctttggcggg cgctggaaaa gggctttgca aatccccgga taatccttag aatgtgcggc
    12661 ctttcgggcc gtgcgcccta gtgtgcatgc agtgtttgca agcaacgact acaggaacga
    12721 agttcaatgg taaccatccg tcttgctcgt ggcggctcca aaaagcgccc tttctatcac
    12781 ctgaccgtga ccaacagccg ctccgcccgt gaaggtcgct tcatcgagcg catcggcttc
    12841 ttcaacccgg ttgctaccgg tggcgaagta cgtctgtccg tgaacgccga acgcgcccag
    12901 tactggctga gccagggtgc ccaggcttcc gagcgcgctg cccagttgct caaggaagcg
    12961 gcgaaagcct gagaccttga tgatgcaaca ttcgccggca cccgctgagg atctggtcgt
    13021 tctcggcaaa atcgtttcgg tgttcggcat ccgcggtgaa gtgaaggtgt attcctacac
    13081 cgacccgttg gagaacctgc tggactaccg tcattggacg ctcaagcgcg atggcgaggt
    13141 ccgccaggtc gaactggtca gtggccgcct gcatggcaag gtactgactg tccggatcaa
    13201 gggactcgac gatcgcgaag aggccctgag ccatgttgga ttcctcatcc ttgtgccacg
    13261 cagtcagctg ccggctctgc aggatgacga gatctattgg tatcagctgg aaggcctgcg
    13321 ggtcatcaac caggacgggc agttgctcgg tcgggtcgat cacttgctgg aaaccggtgc
    13381 caatgacgta ctggtggtga agccctgcgc gggtagtctg gatgatcgtg agcgcctgtt
    13441 gccctatacc gggcaatgcg tgcagcaggt cgatctggct gccggcgaga tgcgggtgga
    13501 ctgggatgcg gatttctaac ccatgcgggt agacgtgctc agcctgttcc ccgagatgtt
    13561 cggcgccatc agcgaatacg gcattaccag tcgcgcggtg aagcagggac tcctgcaact
    13621 gcactgctgg aatccgcggg actacaccga ggaccgccac cagacggtgg acgatcggcc
    13681 cttcggcggt ggccccggca tggtgatgaa gatcaagccg ctggagctgg ccctggcatc
    13741 cgccaagaca gccgcaggcg gtgcggccaa ggtgatctac ctgtcgccgc aaggacgtcg
    13801 tcttgaccag gcggcggttc gcgaattggc gaaagaggag cgtttgatcc tcattgccgg
    13861 gcgttacgaa ggcatcgacg aacgtttcat cgagacgcac gtggatgagg aatggtcgat
    13921 cggtgattat gtcttgtccg gcggtgagct gccggccatg gtactgatcg atgcggttac
    13981 gcggttgctg cccggagcgt tggggcatgc agactccgcc gaggaggact cgtttaccga
    14041 tggcctgctc gattgcccgc actacacccg acctgaggtg tacgagggca gaagtgttcc
    14101 gccggtgctg ctcagcggca accacgaaca catccggcgc tggcgtttgc agcagtccct
    14161 gggacgtacc tgggaacgac gtgccgatct tctggatagt cgctcgcttt ctggagaaga
    14221 gaagaagctg ctggaggaat acatccgcca gcgggacgat agataacgta tcgatggcaa
    14281 gtcccatgac ttgtctttgg agcacagcat gagcaacaag aacctgatca ttcagcagat
    14341 cgaagctgaa cagatgacca aagcgatccc ggccttcgcc cctggcgaca ccgtgatcgt
    14401 gcaagtgaaa gtgaaggaag gcgaccgtga gcgtctgcag gccttcgaag gcgtggtgat
    14461 cgccaagcgc agccgcggcc tgaacagcgc gttcaccgta cgcaagattt ccaacggctt
    14521 cggcgttgag cgtaccttcc agacctacag cccgctggtt gacagcatca acgtcaagcg
    14581 tcgcggcgac gtgcgcaagg ccaagctgta ctacctccgc gcgctgtccg gtcgtgctgc
    14641 acgcatcaag gagaagctgg tctaagaccc gctttcctgc tgaaaaaaag cagccttcgg
    14701 gctgcttttt ttgtgcccgc gattcgtccc gctccggctg gccgtgcgcg ccgtgctcgt
    14761 gccagactgc gggctttatt gctgtggcga ttttcccatg cctgctcccg accatccgct
    14821 gatcgaccgc ttcctcgaga ccctgtggct ggagaagggg ctctcgcggc atacccgttc
    14881 cgcctacggc agcgatctgg cgttgttcca gggctggctg gtggcgcgtg gcttcgatct
    14941 gctgcgcgtc gaccgcgagc tgattcgcga gcatctcggc tggcgtctgg acaatggcta
    15001 tcacgcccgt tccacggcgc ggctgttgtc gtgcctgcgt ggcttctacc atttctgcag
    15061 cttcgagggg ttgctcgccg aggatccgac cctgctggtc tcgctgccgc gccaggcgcg
    15121 ggcggtgccg agctcgctga gcgaggcgga tgtcgaggcg ctgctggcgg cgccggaggt
    15181 gagcgaggcg ctcggcctgc gcgaccgtgc gatgttcgag gtgctctacg cctgcggcct
    15241 gcgggtcagc gaactggtgg cgctgaccct cgagcaggtc aatctgcgcc agggcgtggt
    15301 gcgggtgttc ggcaagggcg cgcgcgagcg cctggtgccg ctcggcgagg aggccatcga
    15361 ctggctgctg cgctacctgc gcgaggcgcg cggcgagctg ctgatcggca agccgagcga
    15421 cgtgctgttt cccagcctgc gcggcgaaca gatgacccgg cagaccttct ggcaccgcat
    15481 caagctgcat gcgcgcacgg cgggcatcga caagccgctg tcgccgcaca ccctgcgcca
    15541 tgccttcgcc acccatctgc tcaaccatgg cgccgacctg cgggtggtgc agatgctgct
    15601 gggtcaccgc gatctgtcga ccacgcagat ctacacccat gtggcccgcg cgcgtctgca
    15661 ggccctgcat gccgagcatc atccgcgcgg ctagccgctc ggcggcgtgt gcgccggcac
    15721 agcgggcggg gtggggttgt atggtaggct tcgccgatca acaagctgcc ttatatcgtt
    15781 gcaaggagtc cccatgcgcc tgttccgttt tctcgctgcc gccacgttcg ggctggccag
    15841 ttccatcgcc ctggctgccg agcccgatca ggccatccgt caggccctga agtcgctgca
    15901 gccggacctg ccgatcgagg cgatcagcga aagcccgatg cccggcctgt accaggtcca
    15961 gctcaagggc ggacgcctgc tctataccag cgccgacgcc cagttcgtcc tgcagggctt
    16021 cctgttccag gtcaaggacg gcaagaccgt caacctcacc gagcaggccg agagcaaagg
    16081 ggtggccaag cagatcaacg ccatcccgca ggccgagatg gtggtgttcc ccgcggccga
    16141 gaaggcggcg cagaagaccc atatcaccgt gttcaccgac accgactgcg gctactgcca
    16201 gaagctgcac agcgaagtcg ccgagctcaa ccgccgcggc gtcgaagtgc gttatgtcgc
    16261 ttttccgcgc cagggcaccg aaagccatgg ctacaacacc ctggtcagcg tgtggtgcgc
    16321 caaggaccgt cgcgaggcga tgaacctggc caaggcgcgc aaggacgtac cggcggcgca
    16381 gtgcgacaac ccggtggcca agcagtacca gctcggccag atgatcggcg tgcagggcac
    16441 tccggccatc gtgctggcca acggccggat gatccccggc taccagccgg caccgcagct
    16501 ggccgaggcc gcgctggcgg ccgcggccgc ggcgaagtga gtgacgggtt gctgcggatt
    16561 gactaacaat cagccgcttc gtaatgtgcg gatcttttca tcggccggca tcgccggccg
    16621 tactgcgtcg taatgatggg gaaactagag tgaaaccggt gaaagtaggt atctgtgggt
    16681 tgggaaccgt cggtggcggc accttcaatg tgctcaaacg caatgccgag gagattgccc
    16741 gccgtgccgg gcgcggcatc gaagtggcgc agattgccgc gcgtgacgcc aacccgcaat
    16801 gcgataccac cggtaccccc atcaccggtg acgtgttcga ggtggcctgc aacccggaaa
    16861 tcgacatcgt catcgagctg atcggtggct acaccgtggc ccgcgagctg gtgctcaagg
    16921 ccatcgagaa cggcaagcac gtggtcaccg ccaacaaggc gctgatcgcc gtgcacggca
    16981 acgagatctt cgccaaggcc cgcgagaagg gcgtcatcgt cgccttcgaa gccgcggtgg
    17041 ccggcggtat cccggtgatc aaggcgatcc gcgagggcct ggccggcaac cgcatcaact
    17101 ggctggccgg catcatcaac ggcaccggca acttcatcct caccgagatg cgcgagaagg
    17161 gccgggcctt cgacgacgtg ctcaaggaag cccaggcact gggctacgcc gaagccgacc
    17221 cgaccttcga cgtcgagggc atcgacgccg cgcacaagct gaccatcctc gcgtccatcg
    17281 ccttcggcat ccccctgcag ttcgacaagg cctacaccga aggcatcacc cagctgacca
    17341 ccgccgacgt caactacgcc gaggcgctgg gctaccgcat caagcacctc ggcgtggccc
    17401 gtcgcaccga ggccggcatc gagctgcgcg tgcacccgac cctgatcccg gccgaccgcc
    17461 tgatcgccaa cgtcaacggc gtgatgaacg cggtgatggt caacggcgac gccgtcggca
    17521 gcaccctgtt ctacggcgcc ggcgccggca tggagccgac cgcctcggcg gtggtggccg
    17581 acgtggtcga cgtgacccgc gtgctgacca cagacccgga gaaccgcgtg ccgcacctgg
    17641 cgttccagcc ggacgcgctg tccgaccacc cgatcctgcc gatcgaggcc tgcgagagcg
    17701 cctactacct gcgcatccag gccaaggacc atccgggcgt gctcgcccag gtggcgacca
    17761 tcctttccga gcgcggcatc aacatcgagt cgatcatgca gaaggaagcc gaggagcagg
    17821 ccggcctggt gccgatgatc ctggtgaccc accgcgtgcg cgagtcccag atgaacgacg
    17881 ccatcgtcgc cctggaagcc ctggaaggcg tatccggcca ggtcgtgcgc atccgcgtcg
    17941 aacaactcaa ttaatccagg cggccagctt ccggcagcgt cgtccgacct gcctgccgct
    18001 ggccgctcag accgaaggtt tgcccccatg cgctatatca gtacccgcgg ccaggcgccg
    18061 gccctgaact tcgaagacgt gctgctggcg ggtctcgcca gcgacggcgg cctctacgtg
    18121 ccggaaaacc tgccgcgctt caccgtcgag gaaatcgcct cctgggccgg cctgccgtac
    18181 cacgagctgg ccttccgggt gatgcgcccg ttcgtcgccg gctgcatcaa tgacgctgac
    18241 ttcaagaaga ttctcgaaga cacctacggc gtgttcgccc accaggcggt ggcgccgctg
    18301 acccagctgg gcgccaacga gtgggtgctc gaactgttcc agggcccgac cctggcgttc
    18361 aaggacttcg ccctgcagct gctcggccgt ctgctcgacc acgtgctggc caagcgcaac
    18421 gagcgcgtgg tgatcatggg cgccacctcc ggcgacaccg gctcggccgc catcgagggc
    18481 tgcaaggcct gcgacaacgt cgacatcttc atcatgcacc cgcacaaccg cgtgtccgag
    18541 gtgcagcgcc ggcagatgac caccatcctc ggcgagaaca tccacaacat cgccatcgaa
    18601 ggcaacttcg acgactgcca ggagatggtc aaggccagct tcgccgacca gggcttcctc
    18661 aagggcaccc gcttggtggc ggtcaactcg atcaactggg cgcggatcat ggcccagatc
    18721 gtctactact tccacgccgc cctgcagctc ggcgcgccgc accgctcggt ggccttctcg
    18781 gtgccgaccg gcaacttcgg cgacatcttc gccggctacc tggcgcgcaa catgggcctg
    18841 ccgatcaacc agctgatcgt cgccaccaac cgcaacgaca tcctgcaccg cttcatgtcc
    18901 ggcaaccgct acgacaagga caccctgcac gcgtcgctgt cgccgtcgat ggacatcatg
    18961 gtctcctcca acttcgagcg cctgctgttc gacctgcacg gccgcaacgg cctcgccgtc
    19021 agcgcgctga tggacgagtt caaggccacc ggcaagctgt cggtcgagga tgaccgctgg
    19081 agcgaggcgc gccgcctgtt cgactcgctg gcggtggacg acgagcagac ctgcgcgacc
    19141 atcgcccagg tgcatgcgca gaccggctac ctgctcgacc cgcacaccgc gatcggcgtc
    19201 aaggccgcgc gcgactgccg ccgcagcctg gcgctgccga tggtggtgct cggcaccgcg
    19261 catccggtca agttcccgga agcggtggag aaggccggca tcgccgccgt gccgcagctg
    19321 ccgccgcacc tggccgacct gttccagcgc gaggagcgct gcacggtgct ggccaacgac
    19381 ctgaagaccg tgcaggactt cgtcagccag cacggcaacc gcggcaagcc gctgtaaggc
    19441 gctgatcgcg agttgtccac agagggccgg ttgcgcaagc aaccggccct cttgtttttc
    19501 cacaggcgaa cactgtccgc ggctgggtcc cggtaggggc agccaagggc agcactgcga
    19561 agttgtgcac aaaatccgca ggtcagtgcc ggcgtttgcg ttcggcgctg ctgcaatggc
    19621 cgcaggttgg ccctaataac taattcattg aaatacaagc agaaaaaaat ctgaacaaaa
    19681 agtaaccaga ttgtctgcag gccatgcatt gcagggggcg gggcgatctg tccggaattt
    19741 gtccacccag ttatccacag taattgtgga ttgaacgcgg caggcggcct gctgcatggc
    19801 cgtcccgacg gcccggtata ctcgccgcta ttgcgtccag gagttcgacc atgcctttcg
    19861 agttgtccag tctgttgctg ggtcttgtcg ccgcggcgct gccagcggcc gccttgctct
    19921 ggcagctgca gcagcaacgc agcctggccc agcagcagct cggccagctg gcggagcgcc
    19981 tgacggccag cgaacaggcg cgcgaggagc tggctgccca gctcggtcag gcgcgcagcg
    20041 aggcacaggg ccagcagcgc cagctgttga ccctgcaggg ggagacggcg gggttgcgcc
    20101 gcgagaccca gctgctgact gtcgagcgcc agcgggccgc cgaggcggcg cagcagtggc
    20161 aacaggagcg ccagcagcgt gaagaggagc tgcgtgacct ggctgcagag cgggcggcgc
    20221 tggcggcagc cctgcgcgag cagcagcagg gccatcgcca gcgtctggag gaggtgcaga
    20281 gcgcgcgcga cgagctgcgc gcgcagttcg ccgagctggc cgggaagatc ttcgacgagc
    20341 gcgagcagcg ctttgccgag agcagccagc agcgcctcgg ccagctgctc gatccgctgc
    20401 gcgagcgcat ccacagcttc gagcagcgcg tcgagcagag ttatcaacag gaggcgcgcg
    20461 agcgtttttc gctgggcaag gagctggagc gcctgcagca actcaaccag cgcctgggcg
    20521 acgaggcgac caacctgacc cgcgcgctca aggggcagaa gacccagggc aactggggcg
    20581 agctggtgct cgagcgggtc ctcgagcacg ccgggctgga gaagggccgc gagtacgaca
    20641 cccaggtcag cctgcgcggc gccggcggcg agcgctacca gccggacgtg ctgatccgcc
    20701 tgcccggcga gcgccaggtg gtggtggacg ccaaggtgag cctgaccgcc taccagcagt
    20761 atgtcggcgc cgaggacgac ggcctgcgtc agcaggccct caagcagcac gtgcagtcgc
    20821 tgcgcagcca cctcaagggg ctctccggca aggattacca gcgcctcgag gggctgcaca
    20881 gcctggactt cgtcctgctc ttcgtgccgc tggagggcgc cttcgccgct gcgctgcaag
    20941 ccgagccggg gctgttcgag gagtccttcg cgcagaacat cgtgatcgtc agtccgacca
    21001 ccctgctggc caccctgcgg gtgatcgaca gcctgtggcg ccaggagcgg caggacctca
    21061 acgcccgcga gatcgccgaa cgcgctggcg cgctgtacga caagttcgtg ttgttcgtgc
    21121 aggacctcga cgagatcggt aaccgcctgc agcagctgga caaggcctac gccaatgcgc
    21181 gcaacaagct caccgccggc cgcggcgacc tgatctcgcg cagcgagcag ctcaagctgc
    21241 tcggtgcgcg cgccagcaag agcctgccgg ccgactggct ggcgcgctcg gccagcctgc
    21301 cgctggcgct ggacgaggat ccggcctgac cggcagcggt ggataacgct gcatccaccc
    21361 tacggttgcg ctgtgcgctc gctgtagggt gcgccgcgcg caccgagagt gctcagagca
    21421 gcaccagcgc caggcccagt cccaggccgc cggccatcac cggcacggtc agcagggcca
    21481 ggcgcgggcc gccgacgatg gcgatcagca cgcccttgat caggctgttg ctgatcaccg
    21541 ccagggcgat gccgcgcact gccacctggg catccagctc gtgcagggcg ctgcgcgaca
    21601 gcgacagggt gatggcatcg acgtcggtca gccctgacag gatggccacc aggtagacgc
    21661 cgacatcacc cagctcgcgg cggccgacct ccaccagcag caggatcacc gccagcagca
    21721 cggcgaagcg cagcgccggg ccgagttcaa aggggttctt cagcggcggt tcggcgcttg
    21781 gcgcctgttc gctgttggcg gcgcgacgcc accagtacaa cgcgccgccg gcgtacacca
    21841 gcgcggcggc gcccagcggc cagagcagtt cggcgagcag atgagggttg atcgcggcca
    21901 cctccagcag cacccgcggg aacatcagcg ccgaggtggt cagcaggccg gccgccagca
    21961 gggcgtgcag ctcgcgctgg ctgtgcaggc gcgagagggt cagggtcatg gcggtggagg
    22021 acaccacgcc accgagcagg gcggtcagca gcaggccgtg gcgggtgccg accaggcgca
    22081 tggcggcgta ggccgcgaag cccagcccgg cgatcagcac caccatccac caggtggcgt
    22141 aggggttgaa cacctgccag gggccgtagc cctggtcggg cagcgccggc agcagcacca
    22201 gcgagatgaa cagcagcttc agcgcgccgg acagttcggc cgcgctgagg cgcttgagcg
    22261 cgctgtgcat cggctccttg aggctgagca gcagggccac caccaccgcg cagccgcccg
    22321 ccagcaggcg actgtgggcc agggccaggc tgccgaggag gaaggtcagc agcagggcca
    22381 tctcgctggt caggccgggg tcgtcatcgt gcttctggtc gatgaggtag gcggtggtca
    22441 ccagcacggc caccgccagc agcaggccga gccaggccag ggggccgaaa tggccggcga
    22501 gcagggtgcc gaggccgccg agcaggccga ccaggccgaa ggtgcggatg cctgcggata
    22561 ggcgggtatc gaggatttcg cgcgcgctcc agccacgctc ggcaccgatc agcaggccga
    22621 tggccaaggc gctggccagg ttcagccagg tgtcgaggtc aggagtcatg ggcaatcctt
    22681 tgggcaggcc ggtacagcca gtctaacagg ccgtcgccag ccccggccgc cgcgcggggc
    22741 ggccggggct ccctcagcgc agattcaggt ggcggctgat cagggcgcgc agggcggccg
    22801 gcttgaccgg tttgctcagg tagtcgagac cgccggcatg cacctcggcg atcagctcgg
    22861 cacgcccgtc ggcgctgatc accacgccgg gcaccggctg gccgaggcgc gcgcgcagcc
    22921 agcccatcag ggccaggccg gtctcgccac cgtccaggtg atagtcgacc aggaccagct
    22981 ccggcgtact gccgccgtcg agcagggcgg cgcattcctc gcgggtccgc gcggtctgca
    23041 cctggcagcc ccagcggctc agcaggctgc gcatgccggt aaggatgctg tcctcgttgt
    23101 cgatgcacca gacctgggcg ccgttcatgg cctggccggc ggtagtctgc aggcgctgtg
    23161 gcgcttgttg ggcgcggcca caggccagcg gcacgcggac gctgaacacg ctgcccttgc
    23221 ccggccagga gcgtacctcg agcttgtgct cgagtacccg gcacagaccg tcggcgatcg
    23281 ccaggccgag gcccaggccc ttttccgcgc gggtctggtg gctgtcgaga cgcttgaact
    23341 cctcgaagat cacctcgagc ttgtccgccg gaatgccggg gccgcggtcc cagacttcca
    23401 gacgcaggtg ctggccatcg cgacgcatgc cgagcagtac cttgcccttg gcgtagcgga
    23461 aggcattggt caggaagttc tgcagcaccc ggcgcagcag cttgctgtcg ctgtcgatgc
    23521 tcagcttgct ggcacggatg cgcaggtcga tgccctgctc ctgggcgagg gcgcggaact
    23581 ccacgccgag ggcgtcgtac agggtggaca gggcgaaggt gctgcgctcc ggggtgaagc
    23641 ggccgccctc caggcgcgag atgtccagca ggtcggcgat caggtcctcg gccgagcgca
    23701 gcgaggagtc caggtggtgc accagctcct gcacctcgac cggcaacgcc tcctggtggg
    23761 cgagggcggc ggagaacagc cgcgcggcat tcagtggctg catcaggtcg tggctgaccg
    23821 cggcgaggaa acggcttttc gactggttgg cgctttccgc acgcgccttg gcctcggaca
    23881 gctgctcgtt cagttcggac agctcgcggg tgcgctcggc gacccgctgc tcgaggctct
    23941 cgttggcctc cttgagcgcg tcctcggcct ggcggtgggc ggtgatgtcg ctgaacgaca
    24001 tgacgaagcc gccgccaggc atcgggttgc cgatcagctc gatcacccgg ccattgggga
    24061 agatgcgctc ggaggtgtgc gcggtgccct ggcgcatcca gtacaggcgc ttggccacgt
    24121 ggaggtccgc atcgcccctg ccgcacaggc cgcgctcggc gttgtagcgg atgatgtcgg
    24181 agatcggccg gccgacactg atcagcccct cggggtactc gaacagttcc agatagcggc
    24241 ggttccaggc caccaggcgc agcgactggt cgaccacgct gatgccctgg gtgatgttct
    24301 cgatcgcgcc ttgcagcagg gcgcggttga actgcaggac ttccgaggcc tcgtcgacga
    24361 tgcgcaccac gtcctcgacc tgcatgtcgc ggccttcgat ggtggccttg accacggcgc
    24421 gcgccgagga ggcgccaacc acgccggcca gcaggcgctc ggtgtgggcg atccactcgc
    24481 tgctggcggt ctggttcgga ctgaagccct ggccctgacg gtaggcgaag cggatgaagc
    24541 tctgccgcgc gcgctcctcg ccgacgaagc gaccggccag ggtcagcagg tcctgcacct
    24601 gcaccgccag caacaggcgc gggctggggc gtaccgaagc ctcctggccg acgaagcggc
    24661 tggcctgcca gtgctcggac acccgggtgc gcgacagcca ggacaccagg gcgaacacca
    24721 cgaagttgcc ggtcagcgac agcagggcgc cctgggtcag cgcatccatg gggatgccca
    24781 gcggattgcc ggccagccag cgctggccgg gaaacaggtc cagcgaccag cccatgcccg
    24841 cggccaccag gggcagcagc agggtgtagc accacagcag cgcgccgacg gccaggccgg
    24901 cgaacacgcc gcggctgttg gcctggcgcc agtacagcgc cccgaacatc gccgggccga
    24961 gctgggtgat ggcggcgaag gcgatctggc cgatgccggc caggctgatg ttcgacccca
    25021 tcatccggta gatcacgtag gccagcagca gcagggcgat gatggcgatg cggcgtaccg
    25081 acagcagcca gtggcgaaac tcctcgaacg gccgttcggc gctgtgccgg cgcagcagcc
    25141 agggcagcag catgtcgttg gagatcatgg tcgacagcgc cacgctggcg acgatgacca
    25201 tgccggtggc ggccgaggcg ccgccgatga aggccagcag ggccagcgcc gggtgggcct
    25261 cggccagcgg caggctgatg acgaaggagt ccggctccac cccggcgggc agcagcagct
    25321 gtccggccag ggcgatgggg atgacgaaca gggcggccag cgcgaggtac agcgggaaca
    25381 cccagcgcgc cacgttgagg tcgcgcggtt cgatgttctc caccacggtg acgtggaact
    25441 gccgcggcag gcagatgatc gccatcatcg ccacgccggt ctgcaccagc atggccggcc
    25501 agtcgagcgg ttgctgccag tagtcctcca gcttcggcgc cgcctgcgcc ttggccagca
    25561 gatcatcgaa gccgtcgaac agcccccagg tgacgaacac gccgacggcg aggaaggcca
    25621 gcagcttgac cagcgactcg aaggccaccg ccatgaccat gccgcggtga tgctcggtgg
    25681 cgtccaggtt gcgggtgccg aacaggatgg tgaacagggc gaggatcagc gacacgatca
    25741 gcgccgtgtc ctgggcgctg atgccgttgg cgtcggcgcg ggcgccggtg agcaggttga
    25801 cgccgaggac gatgcccttg agctgcaggg cgatgtaggg cagcacgccg accaggcaga
    25861 tcagcgccac cacgatggcc agtggccgcg acttgccgta gcgcgcggaa atgaagtcgg
    25921 cgatcgaggt gatgttctcc tgcttgctga tcaggatcat cttggccagc atctgcggca
    25981 tgaccagcag caccagcaat gggccgaggt agatcggcag gaacgaccac agctggccgg
    26041 ccgcctggcc gaccgaaccg aagaaggtcc agctggagca gtacaccgcc agcgacaggc
    26101 tgtagaccca ggcgcgcagg cgcgggcgtt gcggacgggg gctggtctgg cggcgatcgc
    26161 catagaaggc gatggcgaac aggaccgcca tgtaggaaag ggcgaccgcg gtgatcagcc
    26221 cgctggacag cgacatgaga actccggcat ttcagaatcg gcagagtttc gcaccaatgc
    26281 gggtcgacgt caggttcctg cgaccaaggt cgcgggcggc cggcaagcgc tctgccctgc
    26341 gggttgccgg cgtgcggccg gcgagcgggg caatgagggg cgcggcagtg cgccgttgcc
    26401 gtgggcgcca gcactgcagg gagggacggg cgtcgcggcg gacgcccgcc gtggtgtcac
    26461 tgggcgtcga aggcctggcc gttgacgccg gtgctgtccg ggcccatcag atacagatag
    26521 accggcatga tctcttccgg agccgggttc tgctgcggat tttcccccgg ataggcctgg
    26581 gcgcgcatgc tggtgcgggt ggcgccgggg ttgacgctgt tggcacgcac cgcgctgatg
    26641 ccgtcgacct cgtcggccag cacctgcatg atgccctcgg tggcgaactt ggacaccgcg
    26701 taggcgcccc agtaggcgcg ccccttgcgg ccgacgctgc tcgaggtgaa gaccaccgag
    26761 gcgtcgttgg acagcttgag cagcggcagc agcacgctgg tgagcatgaa caccgcgttg
    26821 acgttgacct gcatgacgcg catgaagttg tcgccggaga actgctcgat cggcgtgcgc
    26881 gggccgagca gcgcggcatt gtgcagcagg ccgtcgaggt ggccgaactc gcgctcgatg
    26941 gtggcggcga ggtcgtcgta ctggtgctgc tgggcggttt ccagattgaa cgggaagatc
    27001 gccggctgcg gcccgccggc ggcctcgatc tggtcgtaga cctcttccag gtgctcgatg
    27061 gtcttgccca gcaggatcac cgtggcgcca tgggcggcga aggtgcgcgc ggcggcggca
    27121 ccgatgccac ggccggcacc ggtgaccagg atcacccgat ccttgagcag gtcggggcgg
    27181 gcggaatatt ggaacatggg tagagcctcg ttgaatcgaa atcgcgaaac cggggccggg
    27241 cgtaggctgg gtcagccgct tgcggggtaa cccgccatcg ccaccggcag ctgccgcctg
    27301 cgcggcaagg gtgggtacgc tgcgctaacc cacgctacat tggcatccga gtgtggcgca
    27361 gggttggggt ggccgccatg cgccggaagt cttagagcct ctcagcagcc gcacaaggcg
    27421 cgctcgagca cgtcacgcag ctcgcgggga tgatcgatca ccaggtcggc gccccagtgg
    27481 ccggggttgt cgtcggggtg gatgtagccg tagcgcaccg ccaccgtgcg cgtgccggcg
    27541 gcgcggccgg cctcgatgtc gcgctcgtcg tcgccgacgt agagcacctc gcgcggatcc
    27601 aggtcgagct gctggcaggc cagcagcagc atctccgggt cgggcttgct gcggctgacg
    27661 tgctcggggc agaccagcac cgcggcgcgc tcggccagct gcaggtgctg catgatcggc
    27721 tcggcatagt gcagcggctt gttggtcgcc acgccccagc gcaggcgggc ctgctcgagg
    27781 tcggcgagca gctcggggat gccgtcgaac ggctgggtca gcaccgcgca gtgttgctgg
    27841 tagcgggcga ggaactcctg gcgcaggggc tcgaactcgg gatgctccgg cgtcaggccg
    27901 aaggtgcagg cgaccatggc ccgcgcgccg ccggagacct ggtcgcggat ccgctgcgga
    27961 tccagcggcg gcagttcgcg tgccgcgcgc atggcctggc acaccgcgat gaagtccggc
    28021 gcgctgtcga ccagcgtgcc gtccatgtcg aacagaaccg cccgtagccg catgccgctc
    28081 actcctcgcg cagggtctgg atcatgtagt tgacgtcgac gtcgctaccc agcttgtagg
    28141 tcttgctcag cgggttgtag gtcaggccga cgatgtcctt cacctcgaga ccgctgctgc
    28201 ggcaccaggc gcccagttcg gaggggcgga tgaacttctt gaagtcgtgg gtgccgcgcg
    28261 gcagcatctt cagcaggtac tcggcgccga cgatggccag caggtaggcc ttgggattgc
    28321 ggttgatggt ggagaagaac acctggccgc cgggcttgac caggcgctgg caggcggcaa
    28381 tcaccgaagc cggatcgggc acgtgctcga gcatctccag gcaggtgacc acgtcgaact
    28441 ggccgggcat ctccgcggcc agcgcctcgg cggtgctctg ccggtagtcg acctgcacgc
    28501 cggactccag ctggtgcagg cgcgccacgg ccagcggcgc ctcgcccatg tcgatggcgg
    28561 tgacggtcgc gccgcgcagg gccatcgact cgctgaggat gccgccgccg cagccgacgt
    28621 cgagcacgcg cttgccggcc aggccgacgc gctcgtcgat ccagttgacc cgcagcgggt
    28681 tgatctcgtg cagcggcttg aactcgctct cgcggtccca ccagcgatgg gcgagagcct
    28741 cgaatttggc gatttcggcg tggtcgacgt tgctcatggt gcggttccgt tgggccaagg
    28801 cgggaagctt cagctgtcta tcttgccagt tttcgcgccg cttgggggcg gacggcttgt
    28861 cgcccgggcc gcgaagggtg tcgtagggta gaagactcgc gcagcgatct tctaccgatg
    28921 ggcgggtgcg cggtagcacg cttggtgggt aatcgctgcg cgagttacca ccctacgcct
    28981 tgtcatccct tggcgggccg atcagacccc ggcgatccgc tggccccagg cgcgggcctt
    29041 ggcgcgcagc tcgtcctcgt ccaggcgcgt caggcggcca tcttccagca gcgccttgcc
    29101 gccgacccac aggtgcttca cgcagtcgcg gccgctggca tagatcagtt gcgagaccgg
    29161 gtcgtagacc ggctgctggg ccaggccgct gaggtcgaag gccaccaggt cggcgctctt
    29221 gccggcctcc agcgaaccgc actcgtcgtc gatgcccagc gcgcgggcgc cgttgagggt
    29281 ggccatgcgc agggcgcggt gcgcatccag cgcggtagcc gaaccggcca ctgccttggc
    29341 cagcagggcg gcggtgcggg tctcgccgag caggtcgagg tcgttgttgc tcgccgcacc
    29401 gtcggtgccg atggcgacgt tgaccccggc ctgccacagg cgctccaccg ggcagaagcc
    29461 gctggccagc ttgagattgg actcggggca gtggatcacg ctgctgttgt gctgcaccag
    29521 cagcgccaga tcgctgtcgt cgatctgggt catgtgcacc gcctggaagc gcgggccgag
    29581 caggccgagg cgggccaggc gggccagcgg ccgctcgccg gacagctcga gcgcctgctg
    29641 cacctcgtgg gcggtttcgt gcacgtgcat gtggatgccg gcatccagct gctcggccag
    29701 cacgcgcacg cgctcgagac ggtcgtcgct gaccgtgtag ggcgcgtgcg ggccgaaggc
    29761 gaccttgatc cgcgggtggt ggcgcaggtc ggcgaacagc tcgaggccgc ggcgcagtgc
    29821 ctcgtcggta tcgtgggcgc cgggcaccgg gaaattcagc accggaatgg tgatctgcgc
    29881 gcgcacgccg ctggcgtgca ccacttcgct ggccacctcg gggaagaagt acatgtcgga
    29941 gaaggcgctg atgccgccct tgatctgctc ggcgatggcc aactcgctgc cgtcgcggac
    30001 gaagtcggcg ctcacccact tggcctcggc cggccagatg tgctcctgca gccaggtcat
    30061 cagcggcagg tcgtcggcga ggccgcggaa caggctcatc gccgcgtggc cgtgggcgtt
    30121 gaccagcccg ggggcgagca gcatgcccgg cagctcgcgg cgctcgcggg cggggatggc
    30181 cagcgcctgc tggcgcgggg cgagcagggc gatgcggccg tcgcggatgc ccagcgcgtg
    30241 gtcgcgcagc accaccccgg ccggttccac tgggactatc cattcgggga gcaggagcag
    30301 gtccaggggg gcggtggtgc tgggcatgta cggggcttcc gttcgggcgg ttcgaaggcc
    30361 tgcgagtgta gccgctggcg gggcgctctt gaagcggctg tgcggcccgg caaggggtat
    30421 acttggcggc tttttccgga atccaagagg tagtcccaca tgcgcgagca actgctggct
    30481 gcagagaagg tcacggcaat cgactggcgc cacggcgtcc tgcatctgct cgaccagcgt
    30541 gcgctgccgc tcgaggaaac ctggctggcc tacgacagcg ccgccggcgt ggccgaggcg
    30601 atccgcctga tggtggtgcg cggcgctccg gccatcggca tcgccgccgc ctacggcgtg
    30661 gtgctcggcg tgcgggcgcg gctggcggcc ggcggcgact ggcaggcggc gctggaggag
    30721 gatttccgcg tgctggccga gtcgcggccg accgcggtca acctgttctg ggcgctggat
    30781 cgcatgcgcg agcgcctcaa tcgcctggcc gactgcgacg acgtgctcgc cgcgctggag
    30841 gccgaggccg tcggcatcca cgccagcgac cgcgaggcca acctgaccat ggcccagctt
    30901 ggcgtcgagc tgatccgccg ccacaacagc cacccgcaga agctgctgac ccactgcaac
    30961 accggcgccc tggccaccgg cggcttcggc accgcgctgg gggtgatccg cgccgcgcac
    31021 ctcgccggtc tggtcgagcg ggtattcgcc gacgagaccc gcccctggtt gcagggtgcc
    31081 cggctgaccg cctgggagct ggccggcgag ggcattccgg tgagcctgaa cgccgacgcc
    31141 gctgccgcgc acctgatgaa gaccgagggc atcgcctggg tgatcgtcgg cgccgaccgc
    31201 atcaccgcca acggcgacgt ggccaacaag atcggcacct accagctggc ggtcaacgcc
    31261 atgcaccatg gggtgcgttt catggtggtg gcgcccagct cgaccatcga catgaacctg
    31321 gagagcggcg acgacatccc gatcgaggag cgcgatggcc gcgaactgct ggaaatcggc
    31381 ggccagcgcg tggcggcgga cgtgcatgcg gtcaacccgg tgttcgacgt cacgccggcc
    31441 gacctgatcg atgccatcgt caccgaaaag ggtgtggtcg agcgcccgga tgcggaaaag
    31501 atgaagcagc tgatgtgccg caagcgcctg cactgatcgc cgggctgcag tgcgccggtt
    31561 gactgcgtca cagccctggc cgaggcgccg gactgccgtg tgccggggca ggtggttgtg
    31621 ataagctccg gcggtttata gaggcccgca agggccttcc ggatcgcgcg ttcgggctgc
    31681 ctctggcagt ccgcgcctag aactaaggaa ccaggcttct catgggcgaa ctggccaaag
    31741 aaatcatccc ggtcaatatc gaagacgagc tgaaacagtc gtacctggac tacgcaatga
    31801 gcgtgatcgt cgggcgcgcc ctgccggacg cgcgcgacgg cctcaagccg gtgcaccggc
    31861 gcgtgctgtt cgcgatgagc gagctgggca acgactggaa caagccgtac aagaaatccg
    31921 cccgtgtggt cggtgacgtc atcggtaagt atcacccgca cggcgacacc gcggtgtacg
    31981 acaccatcgt gcgcatggcc cagccgttct cgctgcgcta catgctggtc gacggccagg
    32041 gcaacttcgg ttcggtggac ggcgacagcg ccgccgccat gcgttacacc gaagtgcgca
    32101 tggccaagct ggcccacgaa ctgctggccg acctcgacaa ggaaaccgtc gactgggtgc
    32161 ccaactacga cggcaccgag cagatcccgg cggtcatgcc gaccaagatc cccaacctgc
    32221 tggtcaacgg ctcgtcgggc atcgccgtgg gcatggccac caacatcccg ccgcacaacc
    32281 tgagcgaagt gatcgacggc tgcctggcgc tgatcgacaa cggcgagctg accgtcgacg
    32341 agctgatgca gtacatcccc ggtccggact tcccgaccgc cgcgatcatc aacggtcgcg
    32401 ccggcatcat cgaggcttac cgtaccggtc gcgggcgcat ctacatgcgc gcccgctcga
    32461 tgatcgaaga catcgacaag gtcggtggcc gccagcagat cgtcatcacc gaactccctt
    32521 accagctgaa caaagcccgt ctgatcgaga agatcgccga gttggtcaag gagaagaagc
    32581 tcgaaggtat caccgagctg cgcgacgagt ccgacaagga tggtatgcgc gtcgtgatcg
    32641 agctgcgtcg cggcgaagtg cctgaggtga tcctcaacaa cctctatgcc cagacccagc
    32701 tgcaagcggt gttcggtatc aacatcgtcg cgctgatcga cggtcgtccg cggatcctga
    32761 acctcaagga cctgctggaa gccttcgttc gtcaccgtcg cgaagtcgtt acccgtcgta
    32821 ccgtgttcga actgcgcaaa gcccgcgaac gtggtcacat tctggaaggt caagcggttg
    32881 ccctgtcgaa catcgacccg gtcatcgccc tgatcaaggc ttcgccgacc ccgtcggaag
    32941 ccaaggaagc gctggtcagc accccttggg agtcctcggc ggtagtggcg atggttgaac
    33001 gtgccggcgc cgattcgtgc cgtccggaaa acctcgaccc gcaatacggc ctgcgcgacg
    33061 gcaagtactt cctgtcgcca gagcaggcgc aagccatcct ggaactgcgt ctgcaccgcc
    33121 tgaccggcct ggaacacgaa aagctgctgg ccgagtatca agagatcctc aaccagatcg
    33181 gcgagctgat ccgcatcctc aacagcgcca cgcgcctgat ggaagtgatc cgcgaagagc
    33241 tggaagtgat ccgtgccgaa tacggcgacg tgcgccgcac cgagatcctc gatgcgcgtc
    33301 tcgacctgac cctgggcgac atgatcccgg aagaagatcg cgtggtgacc atttcccacg
    33361 gtggctatgc caagacccag ccactggcgg cgtaccaggc ccagcgtcgt ggcggtaaag
    33421 gcaagtcggc gaccggtgtc aaggatgagg actacatcgc tcacctgctg gttgccaaca
    33481 gccacaccac gctgctgctg ttctccagca agggcaaggt gtactggctg aaaacctacg
    33541 aaatcccaga agcctcccgt gctgcccgtg gtcgtcctct ggtcaacctg ctgccgctgg
    33601 acgacggtga atacatcacc accatgctgc cggtcgagga atacactgaa ggtcacttca
    33661 tcttcatggc caccgccaac ggcaccgtga agaagacccc actggaatcc ttcagtcgtc
    33721 aacgcagcgt cggcctgatc gcgctggagc tggacgaagg cgacgtgctg atttccgcgg
    33781 ccattaccga tggcgagcgc gaagtcatgc tgttctccga cggcggcaag gtaacgcgct
    33841 tcaaggaatc cgacgttcgt gccatgggcc gtaccgctcg tggtgtgcgc ggcatgcgcc
    33901 tgccagaagg tcagaagctg atttccatgc tgatcccgga agaaggcagc caaatcctca
    33961 cggcttcggc gcgtggttac ggcaagcgta cggcgatcac cgagttccct gagtacaagc
    34021 gtggcggcca gggcgtgatc gccatggtca gcaacgagcg caacggccgt ctggtcggtg
    34081 cggtccaggt gctcgacggc gaggaaatca tgctgatttc cgaccagggc accctggtgc
    34141 gtacccgtgt cgacgaagtg tcgagcctgg ggcgtaatac ccagggcgtg accctgatca
    34201 agctggccaa cgatgaaacg ctggttggtc tggagcgggt ccaggagcct tcggaagtcg
    34261 agggtgaaga gctggaaggt gaagaaggtg ctgtgttcga aggcactgca gtgatcgacg
    34321 acatcgtcga agaccagcaa ctcgacgccg ccgctgacga agaaccacag gaataagcgg
    34381 gtaaacaggg ggcggatgaa aattcgcccc cttgttgttt gtccgagagg aaaatgtcga
    34441 cacccatgga tatcccctgt gggagcgagc ctgctcgcga agcaggcgcc gcggtatctc
    34501 tgaaacaccg cggtgatgca ttcgcgagca ggctcgctcc cacaggtgcc tgcttcagca
    34561 gggcccctgt gtcgattgat aatgtgattt attgcgtgat accaccaaat cagagcgaga
    34621 ttggatgtga gcaagagagc ctataacttc tgcgccggtc ctgcggcgct tcccgaagct
    34681 gtcctgcagc gcgcccaggg tgaactcctc gactggcacg gcaagggcct gtcggtcatg
    34741 gaaatgagcc atcgcagcga tgagttcgtg tccattgcca ccaaggccga gcaggatctg
    34801 cgtgatctgc tgaatatccc ctcgaactat aaagtgctgt tcctgcaagg cggcgccagc
    34861 cagcaatttg cccagattcc gctgaacctg ctgcctgaaa gcggcaccgc cgactatatc
    34921 gacaccggta tctggtcgca gaaagccatc gaggaagcgt cgcgcttcgg taacgtcaat
    34981 gttgccgcca ccgccaagcc ctacgactat ttcgccattc ctggccagaa tgaatggcag
    35041 ctgtccaagg acgcggccta cgttcactac gcgccgaacg aaaccatcgg cggcctggaa
    35101 ttccagtggg ttcccgagac cggtgacgtg ccattggtgg ccgacatgtc ttcggacatc
    35161 ctctcgcgcc cgatcgatgt ctcgcgcttc ggcatgatct acgccggcgc ccagaagaac
    35221 atcggcccga gcggtatcgt cgtgaacatc gttcgcgaag acctgctcgg tcgcgcccgt
    35281 tccatttgcc cgaccatgct cgactacaag gtcgcggccg acaacggctc catgtacaac
    35341 accccgccaa ccctggcctg gtacctgtcc ggcctggtgt tcgagtggct gaaagagcag
    35401 ggcggtgtcg aggccatcgg caagctcaat gacgtcaagc agcgcacgct gtacgacttc
    35461 atcgacgcca gcggcctcta cagcaacccg atcaacaagt cggaccgctc gtggatgaac
    35521 gtaccgttcc gcctggccga tgaccgtctg gacaagccgt tcctggctgg cgccgacgag
    35581 cgtggcctgc tgaacctcaa gggccaccgc tccgtgggcg gcatgcgcgc ctccatctat
    35641 aacgccgtcg acatcaacgc gatcaacgcg ctggtggcgt acatggcaga gttcgagaag
    35701 gaacacggct aatgtctgag caagaactca aggcactgcg cctgcgcatc gatgccctgg
    35761 acgaaaaagt cctggagctg atcagtgagc gcgcacgctg cgcccaggaa gttgcgcgag
    35821 taaagatggc ttcgctggcc gaaggcgaag tgccggtgtt ctatcgtccc gagcgcgaag
    35881 ctcaggtgct caagcgtgtc atggagcgca accaggggcc gctgggcaac gaagagatgg
    35941 cgcggctgtt ccgcgaaatc atgtcctcgt gcctggccct cgagcaaccg ctgaaagtcg
    36001 cgtacctggg ccctgaaggc accttcaccc aggcggcggc catgaaacac ttcggccacg
    36061 cggtgatcag caagccgatg gcggccatcg acgaagtgtt ccgcgaagtg gcggccggtg
    36121 cggtgaattt cggcgtggtc ccggtggaaa actccaccga aggcgcggtc aaccacaccc
    36181 tcgacagctt cctcgagcac gacatggtga tctgtggtga agtcgagttg cggattcacc
    36241 accacctgtt ggtcggtgaa aacaccaaga ccgacagcat cagccgcatc tactcccacg
    36301 cccagtccct ggcccagtgc cgcaagtggc tggacgccca ctacccgaat gtcgagcgcg
    36361 tggcggtgtc cagcaacgcc gaagcggcca agcgggtcaa gggcgagtgg aactcggcgg
    36421 cgattgccgg cgacatggcg gccggtctct acgggctgac ccgattggcc gagaaaatcg
    36481 aggaccgtcc ggacaactcc acgcgcttcc tcatgatcgg taaccaggaa gtgccgccga
    36541 ctggcgacga caagacctcc atcatcgtct cgatgagcaa caagccgggc gcgctgcacg
    36601 aattgctggt gccgttccac gacaatggca tcgacctgac gcgaatcgag acccgtccgt
    36661 cgcgcagcgg caaatggacc tacgtgttct tcatcgactt cgtcggccac caccgcgacc
    36721 cgctggtaaa aggtgtgctg gaaaagatca gtcaggaagc agtagcactc aaagtgctgg
    36781 gttcctaccc gaaagcagtt ctctaagggg cggtagcaaa tgagtggcaa tttcctcgct
    36841 ctggcacagc cgggcgtgca acaactttcg ccttacgttc cgggcaaacc cgtggacgaa
    36901 ctggcgcgtg agct
//
LOCUS       DATCUN010000167         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_176605, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000167 DATCUN010000000
VERSION     DATCUN010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176605"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VIO83_06900"
     CDS             complement(<1..751)
                     /locus_tag="VIO83_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEY8331036.1"
                     /translation="MNQPVRRDPVSRFFIGLWNVMNFTRRLIFNLVFFGLLFLVAVLV
                     IIGLAKGETGPRPLLDNTTLVIAPDAALVEQYSSDAASRALAKAFGQEGGEVQLRDLL
                     RVIEAAKADRKINRVLVDFDKLQASGYASLREVAGALADLRASGKQIVAFGENLGQGQ
                     YLLAAQANEVYLDPMGSVVIEGLGRYRQYYRQGLQDKLGVDVHLFRVGEFKSAAEPYI
                     LDAASAESKEADLYWMNDVWNRYLADVGKARR"
     gene            complement(872..1843)
                     /locus_tag="VIO83_06905"
     CDS             complement(872..1843)
                     /locus_tag="VIO83_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY8331037.1"
                     /translation="MHIIGIDVAKATFDIALPLGQGKYRTRSKIANNAQGWEQLLAWR
                     QKHAPEAAVGMEATGIYHQALAEHLVAAGVTVYVINPAQIKAYGQSELLRTKTDRTDA
                     KLIARFFQARQLEPERLRPYLPPTPAQRKLQALVRRLDDLQQMRQMELNRLESANEVL
                     VEGIDQVVSTLDGQIAQIKKAITDHIDEDPDLRDQRNLLHTIPGISGTTSAFLLACLG
                     QLTGYTDVGQLVAHAGLNPAQRQSGKHEDKVRISRIGDATLRTKLYMPALVAKRHNPV
                     LAAFAQRLAEQGKPPKVILCALMRKLLHLVWGVLRSGKPFDPQHAVA"
BASE COUNT          340 a          656 c          721 g          387 t
ORIGIN      
        1 gcctgcgggc cttgcccacg tcggccaggt accggttcca gacatcgttc atccagtaca
       61 ggtccgcttc cttggactcg gccgaggccg cgtcgaggat gtagggctcg gcggcggact
      121 tgaactcgcc cacgcggaac agatgcacgt ccacgcccag cttgtcctgc aggccctggc
      181 ggtagtactg gcggtagcgg cccaggcctt cgatcaccac gctgcccatc gggtccaggt
      241 agacctcgtt ggcctgcgcg gccagcaggt actggccctg gcccaggttc tcgccgaagg
      301 ccacgatctg cttgcccgag gcgcgcaggt cggccagcgc gccggccacc tcgcgcagcg
      361 aggcgtagcc gctggcctgc agcttgtcga aatccaccag cacgcggttg atcttgcggt
      421 cggccttggc cgcctcgatc acccgcagca ggtcgcgcag ctgcacttcg ccgccttcct
      481 ggccgaaggc cttggccagc gcgcggctgg ccgcatcgga ggaatactgc tccaccagcg
      541 ccgcgtcggg cgcgatcacc agggtggtgt tgtccagcag cggccgcgga ccggtctcgc
      601 ccttggccag cccgatgatc accagcaccg cgaccaggaa cagcaggccg aagaagacca
      661 ggttgaagat cagccggcgg gtgaagttca tcacgttcca caggccgatg aaaaatcggc
      721 tgacgggatc gcggcggacg ggctggttca ttggcatgcg cgctctgtac aggaactggc
      781 cgccagctta gccgctggat ggcgccatcg gcatgcccca cacgtcatga cacctgtcgt
      841 gggagccgcg tagataccgt cttgcggtgg tctaggcaac ggcatgctgc gggtcgaagg
      901 gcttgcccga tcgcagtacg ccccagacca ggtgcaggag ttttcgcatg agtgcgcaga
      961 ggatgacctt gggcggcttg ccctgttcgg ccaggcgctg ggcaaaggca gccaggaccg
     1021 ggttgtggcg cttggctacc aaggcgggca tgtagagttt ggtgcgcaag gtggcatcgc
     1081 cgatacgcga gatgcggacc ttgtcctcat gcttgccgga ctggcgctgg gctgggttca
     1141 ggccggcatg ggccaccagt tggccgacgt cggtgtagcc ggtgagctgg cccaggcagg
     1201 ccagcaggaa ggcgctggtg gtgccggaga tgccgggaat ggtgtggagc aggttgcgct
     1261 gatcgcgcag gtccgggtcc tcgtcgatgt gatcggtgat ggccttcttg atctgggcga
     1321 tctggccatc cagggtagac accacctgat cgatgccctc gacaaggacc tcgttggcgc
     1381 tctccaggcg gttgagctcc atctgccgca tctgctggag atcatccagc cggcgcacca
     1441 gggcctgcag tttgcgctgg gccggtgtcg ggggcagata gggccgcagc cgctccggtt
     1501 cgagctggcg cgcctggaag aagcgggcga tgagcttggc gtcggtgcga tcggtcttgg
     1561 tgcgcaacaa ttcgctctgg ccataggcct tgatctgggc cggattgatc acgtacacgg
     1621 tcaccccggc ggcgaccagg tgctcggcca gggcctggtg gtagatcccg gtggcctcca
     1681 tgcccacggc cgcttcaggt gcatgcttct ggcgccaggc cagcagctgt tcccaccctt
     1741 gcgcattgtt ggcgatcttg cttcgggtgc ggtacttgcc ctgccccagg ggcagggcga
     1801 tgtcgaaagt ggccttggcc acgtcaatgc cgatgatgtg catttgcttg cgcctatttc
     1861 cgattgcagg acttgatcaa cagaaaccgc catgcttgcc cttgtcagtg caggctcacg
     1921 ccgaagcggg ccgaagatac cgttcaagct ttcaggcagt gatggttccg gtgcgccgaa
     1981 atctacgaca caagctcgat ggcttcaggg cgggtacggc gtcactggaa cctcccggag
     2041 atcaccgggg aagtcggcct gatcagggcg gacttcaaga cacaagggcg ggtcttgacc
     2101 cgcc
//
LOCUS       DATCUN010000168         7023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_269868, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000168 DATCUN010000000
VERSION     DATCUN010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269868"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3886)
                     /locus_tag="VIO83_06910"
     CDS             complement(<1..3886)
                     /locus_tag="VIO83_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY8331038.1"
                     /translation="MEHSTASRIASRFAGLPADKRREFLAKMQEQGVSFGQLPIPPAT
                     TPQATFELSYAQQRQWFLWQLDPQSSAYNIPVALRLQGPLNLAALQQSFDVLLERHQS
                     LRSRFVEEDGQVMQALASFNTLPIEQHDLRAEPEALRLESAMKKVEAEIARPFDLQHG
                     PLLRVSLLQLDVDDHVLVLTLHHIVTDGWSMGVLVEEFSRLYAAHCQGQSAGLEALPI
                     QYSDYAAWQRRWMEAGELERQLDWWREKLGSEQPLLELPIDRPRPAQPSQRGARLDFA
                     LDASLATGLMSLARQRGVTPFMLLLASFQALLYRYSGQSDLRIGVPIGNRNRAETRGL
                     IGFFVNTQVMRAELDGRLPFAQLLEQTRAFSLDAQDHQDLPFERLVQALQGERSLSHS
                     PLFQVMFNHQQAKPAAVSGLLAGLRVEVLNATAHTTQFDLQLDTQEEGDKLFASLTYA
                     TDLFDAERIEHLARHWRNLLAGVLANPQTPLAELPLLDADEHLRILDDLNDTAQDYPG
                     EVCVQRHFETRVMENPQATALVFQGQSLSYAELNLRANRLAHHLRAQGVGPEVIVGIA
                     CERSLEMVVGLLAILKAGGAYVPLDPQYPLDRLGYMIEDSGISLLLTQEHLLAQLPTP
                     DSVRTLCLQAHSEWLSELPGDDLPNLAVGENLAYMIYTSGSTGKPKGAGNRHVALHNR
                     LEWMQQAYNLLPSDRVLQKTPFSFDVSVWEFFWPLMKGATLVIAAPGEHRDPQRLAAL
                     IVEQAITTLHFVPSMLSAFIAADEALACTSLTRIVCSGEALPMELQRQTLRSLPQAHL
                     YNLYGPTEAAIDVTHWTCVEEGRDSVPIGRPIGNLRTLILDSELQPMPQGAVGELYLG
                     GIGLARGYHRRGALTAERFVADPFGSGERLYRTGDLARYRADGAIDYCGRIDHQVKIR
                     GLRIELGEIEARLQQHAGVQEAVVLALDGPSGKQLVAYIVPQDRALLSADAQQQCAWR
                     ETLKSHLLGSLPDYMVPAQTVLIEHMPLSPNGKLERKRLPAPTAQVSQRAFEAPRSEH
                     ERVLAQIWQDVLGLEQVGRQDNFFELGGDSIISIQVVSRARRAGLSLQPRDLFQQQTL
                     QALAAVVKEQAAPLAQQGSVDGVQTLTPIQRWFFEEPIPQRAHWNQAVLLTPRETLEL
                     PRLQGALQRVLKQHDALRLRFSQVDGQWSARYVGADSADVIDMLWTARVASDASLESV
                     CDEAQRSLSLQDGPLLRVALIAQADGSQRLLLVIHHLAVDGVSWRVLLEDLQAAYQGQ
                     DLPPKTSAFQAWADKLNAYAKSETAMNELSWWQ"
     gene            complement(3886..>7023)
                     /locus_tag="VIO83_06915"
     CDS             complement(3886..>7023)
                     /locus_tag="VIO83_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020795706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY8331039.1"
                     /translation="HSAAYNIPAALRLKGPLNRRALRTTFAQLQVRHETLRTTFEQKG
                     QQARPVVHVELPLQFNEQPINESAINTAVAAEIARPFDLRHGPLWRCLLLHVSAEDHV
                     LVLTVHHIAADGWSMQVMVDEFSALYQAAVQERAANLQALPVAYSDYARWQRDWLEAG
                     EGARQLAWWREQLSGSQAPLELPSELTRPARRSERGASLALNIDRQLLAGLRERAQEQ
                     QVTLFMLLLASFQTLLHRQSGQSGISVGVPSAGRSRLETEGLIGFFINTQVLRAEIDG
                     QQPFSALLQQVKQTALDAQAHQDLPFEQLVDALQPDRSLNHSPLFQVLYNHQQKLGAS
                     VERAVADLQVERLHWQQHTAQFDLVLDTQEQGDELDANLSYATDLYDEASMQRFAEQW
                     LNLLRAVVENPQQRVAELPLLQAPQYGELIQHWNPTFEAQAQSPTLHQLFEAQAAQRP
                     HAIALTCEGEQMSYAALNAEANRLARKLREQGVSPEVRVGIATERAIPLVVGLLAILK
                     AGGAYVPLDPQYPVERLSYMIDDSGIGLLLTQSHLIDGLPAREGVQVLDLTALQLDRY
                     SADNLAPLASPDNLAYVIYTSGSTGRPKGALLSHGNVGRLLTATAGEFDFGVNDVWTL
                     FHSYAFDFSVWELFGALCTGGRLVIVPYYISREPQAFHRLLCDEGVTVLNQTPTAFRQ
                     LLPIACASPRSLALRQVIFGGEALEVASLRSWFERFGDQQPTLVNMYGITETTVHVTY
                     RAIRLADLDAKAQSPIGLPIRDLRWYLLDSQLQPVPVGVAGELYIAGAGLARGYHGRS
                     GLTAERFVPSPFDAAQRLYRSGDLARQRADGSIDYLGRIDQQVKIRGFRIELGEIEAA
                     VLAQPGVRQAVISVHVDDGGPQLCAYVVAELTSNDPIAWRDTLRAALSVDLPDYMVPR
                     HWLLLDALPLTSNGKLDRKALPLPDAETWQRPFEAPEGELEQQLAAIWAEVLGVARIG
                     RRDNFFELGGHSLLAAQASARVELELGIELPLRVLFESNDLQAYAANAWQHAPIDNDA
                     RLDALESLLDEMEIN"
BASE COUNT         1216 a         2215 c         2313 g         1279 t
ORIGIN      
        1 tctgccacca gctcaattca ttcatggccg tttcggactt cgcataagca ttgagcttgt
       61 ccgcccaggc ctggaaggca ctggtcttgg gcggcaggtc ctggccttga tacgcggcct
      121 gcaaatcttc cagcagcacg cgccaggaca ccccgtccac cgccaggtgg tggatgacca
      181 acagcaaacg ttgcgaacca tcggcctggg cgatcagcgc cacgcgcagc agcggcccgt
      241 cttgcaggct gaggctgcgc tgggcttcat cgcacaccga ttccagcgac gcatcactgg
      301 cgacgcgggc agtccagagc atgtcgatga catcggcgct gtcggcgccg acataacgcg
      361 ccgaccattg gccgtcgacc tggctgaaac gcaagcgcag ggcatcgtgc tgcttgagca
      421 cacgttgcag ggcgccttgc aggcgcggca gttcgagggt ttcgcgcggc gtcagcaaca
      481 ccgcctggtt ccagtgggcc cgctgcggga tcggttcttc gaagaaccag cgctggatcg
      541 gcgtgagtgt ttgcacgcca tccaccgacc cttgctgcgc cagcggtgcg gcttgttctt
      601 tcaccaccgc ggccagggct tgcagggtct gttgctggaa caggtcgcgc ggttgcaggc
      661 tcaggccggc acggcgggcg cggctgacca cctggattga aatgatcgag tcgccgccca
      721 gttcaaagaa gttgtcctgg cggccgacct gctccaggcc cagcacgtcc tgccagattt
      781 gcgccagcac gcgctcatgt tcgctgcgtg gtgcttcgaa cgcgcgctgg ctgacctgcg
      841 cggtcggtgc cggcaggcgc ttgcgctcga gtttgccgtt ggggctcagg ggcatgtgtt
      901 cgatcagcac ggtctgcgcc ggcaccatgt agtccggcag gctgccgagc agatgacttt
      961 tcagggtttc gcgccaggcg cattgctgct gcgcatcggc gctgagcaac gcgcgatcct
     1021 gcggcacgat gtaggccacc agttgcttgc cgctcgggcc gtcgagggcc agcaccacgg
     1081 cttcctgtac gccggcatgt tgttgcaggc gcgcttcgat ttcccccagt tcgatgcgca
     1141 agccgcgaat cttcacctga tggtcgatcc gaccgcagta atcaatggca ccgtcggcgc
     1201 ggtagcgcgc caggtcaccg gtgcggtata gacgctcgcc gctgccaaac gggtcggcca
     1261 cgaaacgctc ggcggtcaac gcgccacggc ggtgataacc ccgtgccagg ccgataccgc
     1321 ccaggtacag ttcgcctacc gcgccctgcg gcatgggttg cagttcgctg tcgaggatca
     1381 aggtgcgcag gttgccgatc ggccggccga tcggcacgct gtcgcggccc tcttccacgc
     1441 acgtccagtg ggtcacgtcg atggccgctt cggtcgggcc atacaggttg tacagatggg
     1501 cttgcggcag gctgcgcagg gtctggcgtt gcagctccat cggcagcgct tcgccactgc
     1561 agacgatgcg ggtcagcgag gtgcaggcca gcgcctcatc ggcagcgatg aaggccgaga
     1621 gcatcgacgg cacgaagtgc agcgtggtga tggcttgctc gacgatcagt gcagcgaggc
     1681 gttgcggatc gcgatgctcg cctggagcgg cgatcaccag cgtcgcgcct ttcatcagcg
     1741 gccagaaaaa ctcccacacc gacacgtcga aactgaacgg tgttttttgc agcacgcggt
     1801 cgctcggcag caagttgtag gcctgctgca tccactcgag gcggttatgc agggctacat
     1861 gacggttgcc cgcacctttc ggttttccgg tggaaccgga ggtgtagatc atgtaggcga
     1921 gattttcgcc gacagccagg ttgggcagat catcgcccgg cagttcgctc agccactcgg
     1981 aatgcgcttg caggcagagg gtgcgcacgc tgtccggcgt cggcagttgc gccagcaggt
     2041 gttcctgggt caacagcagg gaaatgccgc tgtcctcgat catgtagccc aggcgatcga
     2101 gcgggtattg cgggtccagc ggcacgtagg cgccgccggc cttgaggatc gccagcaggc
     2161 cgacgaccat ttccagcgaa cgctcgcagg cgatcccgac gatcacttcc gggccgacac
     2221 cctgggcgcg caagtggtgg gccaggcggt tggcgcgcag gttgagctcg gcgtagctca
     2281 gggactggcc ctggaacacc agcgccgtcg cctgtggatt ttccatcact cgcgtttcga
     2341 aatgccgctg tacgcagact tcgcctggat agtcttgagc ggtgtcgttg aggtcgtcga
     2401 ggatgcgcag gtgctcgtcg gcatcgagca acggcaattc ggccagcggg gtttgcgggt
     2461 tcgccaacac cccggccagc aggttgcgcc aatgccgggc caggtgctcg atgcgttcgg
     2521 cgtcgaacag gtccgtggcg taggtcaggc tggcgaacag cttgtcgccc tcttcctggg
     2581 tgtccagctg caggtcgaac tgggtggtgt gcgccgtggc gttcagcacc tcgacacgca
     2641 ggccggcgag caagccgctg accgctgcgg gtttggcctg ctggtggttg aacatcacct
     2701 ggaacaacgg gctgtggctc aagctgcgtt cgccttgcag ggcctgcacc aggcgctcga
     2761 acggcaagtc ctgatggtcc tgggcatcca gcgaaaaggc gcgggtctgc tcgagcaact
     2821 gagcgaacgg cagccggcca tcgagttcgg cgcgcatcac ctgggtgttg acgaagaagc
     2881 cgatcaggcc acgggtttcc gcgcgattgc ggttgccgat cggcacgcca atacgcaggt
     2941 cggactggcc gctgtagcga tagagcaagg cctggaaact ggccagcaac agcatgaatg
     3001 gcgtgacgcc gcgctgtctg gccaaggaca tcaggccggt ggcgagcgag gcatccaagg
     3061 cgaagtccag acgagcgccg cgctggctcg gttgtgccgg acgcgggcga tcgatgggca
     3121 actccagcaa cggctgttcg ctgccgagct tttcacgcca ccaatcgagt tgacgttcaa
     3181 gctcgccggc ttccatccag cgccgctgcc aggccgcata gtccgaatac tggatcggca
     3241 acgcttcaag tccagcgctc tggccttggc aatgggccgc gtagagcctt gagaactctt
     3301 cgaccagcac gcccatcgac cagccatcag tgacgatgtg atgcagggtc agcaccagca
     3361 catggtcatc aacgtccagc tgtaacaagc tgacgcgcaa cagcgggccg tgttgcaagt
     3421 cgaacggacg ggcaatctcc gcctcgactt ttttcatcgc cgattcaagg cgtagcgctt
     3481 cgggttcggc gcgcagatcg tgctgttcga tgggcagcgt gttgaagctc gccagggctt
     3541 gcatcacctg gccgtcttcc tcgacaaaac ggctgcgcag actctgatga cgctccagca
     3601 gcacgtcgaa actctgttgc agcgccgcaa ggttcagcgg cccctgcaga cgcagggcca
     3661 caggaatgtt gtaggcgctg ctctgcgggt cgagctgcca caagaaccat tgacgctgct
     3721 gcgcatagga caattcgaaa gtcgcttgcg gcgtggtggc gggcgggatc ggcagctggc
     3781 cgaagctcac gccctgctcc tgcatttttg ccagaaactc acggcgcttg tcggccggca
     3841 ggccagcgaa gcgggaggcg atacgcgagg cggtgctgtg ttccatcagt taatctccat
     3901 ctcgtccaga agcgactcaa gcgcatccag gcgtgcatcg ttgtcaatcg gggcgtgttg
     3961 ccaggcgttg gcggcataag cctgaaggtc gttactttcg aacagcacgc gcagcggcag
     4021 ctcgatgccc agttccagtt ccacgcgggc actggcctgg gcggcaagca gcgaatgccc
     4081 gcccaattca aagaagttgt cacgccgccc gatccgggcc acgcccaaca cctcggccca
     4141 gatcgccgcc agctgctgtt cgagctcgcc ctcgggggct tcgaacgggc gctgccaggt
     4201 ttcggcatcc ggcagcggca aggccttgcg gtcgagcttg ccgttgctgg tcagcggcaa
     4261 ggcatcgagc aacaaccagt gcctaggcac catgtaatcc ggcagatcga cgctgagtgc
     4321 ggcgcgcagg gtgtcgcgcc aggcgatcgg atcattcgaa gtgagttcgg cgacgacgta
     4381 ggcgcacagc tgcggaccgc cgtcatcgac atgcacgctg atcaccgcct gacgcacgcc
     4441 cggttgcgcc agcacggccg cttcgatctc gcccagttcg atgcggaacc cgcggatctt
     4501 cacttgctgg tcgatccgcc ccaggtagtc gatgctgccg tcggcgcgct ggcgcgccag
     4561 gtcgccgctg cgatacaggc gctgcgccgc atcgaagggg cttggcacga aacgctcggc
     4621 ggtcaggccg ctacgcccgt gataaccgcg cgccaggccg gcgccggcga tgtacagctc
     4681 ccccgccacg cccaccggta ccggttgcaa ctggctgtcg agcaagtacc agcgcaggtc
     4741 gcggatcggc aggccgatcg ggctttgcgc cttggcatcg aggtcggcca ggcggatcgc
     4801 gcggtaggtc acgtgcaccg tggtctcggt gatgccgtac atattcacca gcgttggttg
     4861 ttggtcaccg aagcgttcga accaggaacg caagctggcc acttccagcg cttcgccacc
     4921 gaagatcacc tggcgcaacg ccaggctgcg cggactggcg caggcaatcg gcaacaactg
     4981 gcggaacgcg gtcggggtct ggttcagcac cgtcacccct tcatcgcaga gcaagcgatg
     5041 aaacgcctgc ggttcgcgac tgatgtagta cggcacgatc accaggcgcc caccggtgca
     5101 caacgcgccg aataactccc acaccgagaa atcgaaggcg taggagtgga acagggtcca
     5161 gacatcgttc acgccgaaat cgaactcgcc agcagtggcc gtgagcaggc gcccgacgtt
     5221 gccatggctg agcaaggcgc cttttgggcg gcctgtggag ccggaggtgt agatcacgta
     5281 ggccagattg tccgggcttg ccaacggcgc caggttgtcg gcgctgtagc ggtccagctg
     5341 caacgccgtc agatcaagaa cctgcacgcc ttcgcgggcc ggcaagccgt cgatcaggtg
     5401 agattgggtc aacagcaacc caatgccgct gtcatcgatc atgtagctca ggcgctcgac
     5461 cgggtattgc gggtccagcg gcacgtaggc gccaccggcc ttgagaatcg ccagtaagcc
     5521 gaccaccagc ggtattgccc gctcggtggc gataccgacc cgcacttcgg ggctgacacc
     5581 ctgctcgcgc agtttgcgcg ccaggcggtt ggcctcggcg ttgagcgcgg cgtagctcat
     5641 ctgctcgcct tcgcaggtca gtgcgatggc gtgtgggcgt tgcgcggcct gggcttcgaa
     5701 cagctgatgc aaggtgggcg attgcgcttg tgcctcgaag gtcggattcc agtgctggat
     5761 caattcgccg tattgcggcg cttgcagcag cggcaattcg gcgacgcgct gctgtgggtt
     5821 ttcgaccact gcgcgcaaca ggttcaacca ctgttcggcg aagcgctgca tcgaggcctc
     5881 gtcgtacagg tcggtggcgt agctcaggtt ggcgtccagt tcatcgccct gctcctgggt
     5941 atcgagcacc agatcgaatt gcgcggtgtg ctgctgccag tgcaggcgct cgacctgcaa
     6001 atcggccacc gcgcgctcga cactggcgcc gagtttttgc tgatggttgt agagcacctg
     6061 gaacaacgga ctgtgattga ggctgcggtc cggttgcagt gcatcgacca gttgctcgaa
     6121 cggcaagtcc tgatgagcct gcgcgtccaa tgccgtttgc ttgacctgtt gtagcaaggc
     6181 gctgaacggt tgctgcccgt cgatctcggc acgcagcacc tgggtattga tgaagaagcc
     6241 gatcaggcct tcggtttcca ggcgtgaacg ccctgcactc ggcacgccga cgctgatgcc
     6301 cgactgcccg ctctggcggt gcagcagggt ctggaaactt gccagcaaca gcatgaacag
     6361 cgtcacttgc tgttcttgcg cacgctcgcg caaaccggcc agcagctggc gatcgatgtt
     6421 caacgccagg ctggcaccgc gctcgctgcg ccgggccgga cgggtcagtt cacttggcaa
     6481 ctccagcggt gcctggctgc cggacagttg ctcgcgccac cacgccagtt gccgcgcacc
     6541 ctcgccggct tccagccagt cgcgctgcca acgggcgtag tcgctgtaag ccaccggcag
     6601 tgcctgcagg ttggcagcac gctcttgcac ggcagcctga tacagcgcgc tgaattcatc
     6661 gaccatcacc tgcatcgacc agccatcggc ggcgatgtgg tgcacggtaa gcaccagcac
     6721 atggtcttcg gcgctgacgt gcagcagcaa acaacgccac agcgggccat ggcgcagatc
     6781 gaacggccgg gcgatttctg cggccaccgc agtgttgatt gccgactcat tgatgggctg
     6841 ttcattgaac tgcaacggca gctcgacatg caccaccggg cgcgcctgct ggcccttctg
     6901 ttcgaaggtg gtgcgcaggg tttcgtggcg aacctgtaac tgggcgaagg ttgtgcgcag
     6961 ggcccggcgg ttcagcgggc ctttgaggcg cagcgccgcc gggatgttgt aggccgcgct
     7021 gtg
//
LOCUS       DATCUN010000169         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_194957, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000169 DATCUN010000000
VERSION     DATCUN010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194957"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1898)
                     /gene="betT"
                     /locus_tag="VIO83_06920"
     CDS             complement(<1..1898)
                     /gene="betT"
                     /locus_tag="VIO83_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HEY8331040.1"
                     /translation="METSQRPGATLNPPVFFTSVLLILALVVFAVAFPDSAQSLFSLV
                     QGWIITNASWFYILAVALILITTVFLAVSRYGDIKLGPDHSQPDYSNTTWFAMLFSAG
                     MGIGLMFFGVAEPVMHFVAPPVGEPHSVQAAREAMKLTFFHWGLHAWAIYAIVALILA
                     FFCYRHGLPLTLRSALYPLIGERIHGPIGHAVDIFAILGTVFGVATSLGYGVLQINSG
                     LNHLFGLPVGTWVQIGLIVGVTALATLSVVTGLDKGIRRLSELNLALAAILLLLVLVL
                     GPTVYLLQAFVQNTGSYLSEIVSKTFNLYAYEPTDWLGGWTLLYWGWWMSWSPFVGMF
                     IARVSRGRTIRQFISGVLFVPAGFTLLWMTVFGNGAIHMIMQQGLTSLAETVGQDSSL
                     ALFAFLEQFPFASVLSLLAVLMVVVFFVTSADSGSLVVDMLASGGSNHTPLWQRIFWA
                     SSIGVVAIALLLADGLSALQTATIASALPFSVILLASMWGLLKALHLDATKRVLRHQT
                     SGHTRQARSPGGWQRRLRNMVMFPRRAHVNRFLDEVVQPACLAVTEELRKQGLTAEVQ
                     SGDDHRVRLEVRHGAEVDFVYEVHPRAYVQPSFVMLEGDEERKYFRAEVHLLEGGQDY
                     DVMGWSRD"
     gene            complement(2093..>2372)
                     /locus_tag="VIO83_06925"
     CDS             complement(2093..>2372)
                     /locus_tag="VIO83_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009395817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEY8331041.1"
                     /translation="AQQVAAAQQRQLERQGCANAHLDLPGLRQHCPLSDADRLWLESA
                     GERLRLSLRALHRVLKVARTLADLEGLADIQRAQLAEALQYRAASEPG"
BASE COUNT          419 a          739 c          879 g          335 t
ORIGIN      
        1 tcgtcgcgcg accagcccat cacgtcgtag tcctggccgc cctcgagcag gtgcacctcg
       61 gcgcggaagt acttgcgctc ctcgtcgccc tcgagcatga cgaagctcgg ctgcacgtag
      121 gcgcgcggat gcacctcgta gacgaagtcc acttccgcgc cgtggcgcac ctccagacgc
      181 acgcggtggt cgtcgccgct ctgcacctcg gcggtcaggc cctgcttgcg cagttcctcg
      241 gtcaccgcga gacaggccgg ctgcaccacc tcgtcgagga agcggttgac gtgggcgcgg
      301 cgcgggaaca tcaccatgtt gcgcaggcgg cgctgccagc cgccggggct gcgcgcctgg
      361 cgcgtatgcc cggaggtctg gtggcgtagc acgcgcttgg tggcgtccag gtgcagggcc
      421 ttgagcagcc cccacatcga ggccagcagg atcaccgaga acggcagggc gctggcgatg
      481 gtggcggtct gcagggcgct gaggccgtcg gccagcagca gggcgatggc caccacgccg
      541 atcgagctgg cccagaagat ccgctgccac agcggggtgt ggttgctgcc gccggaggcg
      601 agcatgtcga ccaccagcga gccggagtcg gccgaggtga cgaagaacac caccaccatc
      661 agcaccgcca gcagcgacag caccgaggcg aagggaaact gctcgaggaa ggcgaacagc
      721 gccagcgagc tgtcctgacc gaccgtctcg gccaggctgg tcaggccctg ctgcatgatc
      781 atgtggatgg cgccgttgcc gaagacggtc atccacagca gggtgaagcc ggccgggacg
      841 aacagcacgc cgctgatgaa ctggcggatg gtgcgcccgc gcgacacccg ggcgatgaac
      901 atgccgacga agggcgacca ggacatccac cagccccagt acagcagggt ccagccgccc
      961 agccagtcgg tcggctcgta ggcgtacagg ttgaaggtct tgctgacgat ctccgacagg
     1021 tagctgccgg tgttctgcac gaaggcctgc agcaggtaca ccgtggggcc gaggaccagc
     1081 accagcagga ggaggatggc ggccagcgcc aggttcagct cggacaggcg gcggatgccc
     1141 ttgtccagcc cggtgaccac cgacagggtg gccagcgcgg tgacgccgac gatcaggccg
     1201 atctgcaccc aggtgccgac cggcaggccg aacaggtggt tgaggccgct gttgatctgc
     1261 agcacgccgt agccgagcga ggtggccaca ccgaacaccg tgccgaggat ggcgaagatg
     1321 tccaccgcgt ggccgatggg gccgtggata cgttcgccga tcagcgggta gagcgccgag
     1381 cgcagggtca gcggcaggcc gtggcggtag cagaagaagg ccaggatcag cgcgacgatg
     1441 gcgtagatcg cccaggcgtg caggccccag tggaagaagg tcagcttcat cgcctcgcgg
     1501 gcggcctgca cgctgtgcgg ctcgcccacc ggcggggcga cgaagtgcat caccggctcg
     1561 gcgacgccga agaacatcag gccgatgccc atgccggcgg aaaacagcat ggcgaaccag
     1621 gtggtgttgc tgtagtcggg ctggctgtgg tccgggccga gcttgatgtc gccgtagcgg
     1681 ctgacggcga gaaataccgt ggtgatcagg atcagggcga cggcgaggat gtagaaccag
     1741 ctggcgttgg tgatgatcca gccctgcacg aggctgaaca ggctctgggc gctgtccggg
     1801 aaggccacgg cgaacaccac cagggcgagg atgagcagga ccgaggtgaa gaacaccggc
     1861 gggttgaggg tggcgccggg gcgctgggat gtttccattg agcaagctct ccgtatgagc
     1921 ggatggccgc caagggcgga tagcagacag actggaccgg ccgccagggc tgcgcaaacg
     1981 cgcgcagaga cccgtcggca ggaggttgtg gccggccatt ctaacaagct gatagtttta
     2041 attgtggtta tagttatttc ttatagcttg gctgtatcgg cagggcggct gctcagccgg
     2101 gctcgctggc tgcgcggtac tgcagcgcct cggccagctg ggcgcgctgg atgtcggcga
     2161 ggccttcgag atcggcgagg gtgcgcgcca ccttgagcac ccggtgcagg gcgcgcagcg
     2221 acaggcgcag gcgctcgccg gcactctcca gccacagacg gtcggcgtcg ctcagcgggc
     2281 agtgctggcg caggccgggc aggtcgaggt gggcgttggc gcagccctgg cgctcgagct
     2341 ggcgctgctg ggcggcggcc acctgctgtg cc
//
LOCUS       DATCUN010000170         3531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_25909, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000170 DATCUN010000000
VERSION     DATCUN010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25909"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VIO83_06930"
     CDS             complement(<1..197)
                     /locus_tag="VIO83_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8331042.1"
                     /translation="MSIEIRNVSKRFGAFQALNDINLDIHSGELVALLGPSGCGKTTL
                     LRIIAGLETPDAGSIAFHGEDV"
     gene            complement(201..1076)
                     /gene="cysW"
                     /locus_tag="VIO83_06935"
     CDS             complement(201..1076)
                     /gene="cysW"
                     /locus_tag="VIO83_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEY8331043.1"
                     /translation="MSSVTAASASAHANAARRGHALGRNLLIVSAWLVFALFLLLPLY
                     VVLSEALKLGLGTFFTAIVEPDAIAALQLTLLAVAISVPLNLVFGVAAAWCVSKYEFR
                     GKSLLVTLIDLPFSVSPVIAGLIYVLLFGAQGFFGEWLSERDVQIVFAVPGIVLATLF
                     VTVPFVARELIPLMQEQGTQEEEAARLLGANGWQMFWHVTVPNIKWGLIYGVVLCTAR
                     AMGEFGAVSVVSGHIRGVTNTLPLHVEILYNEYNHVAAFSVASLLLALALFILLLKQW
                     SEARLARLKSNAAEE"
     gene            complement(1090..1908)
                     /gene="cysT"
                     /locus_tag="VIO83_06940"
     CDS             complement(1090..1908)
                     /gene="cysT"
                     /locus_tag="VIO83_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEY8331044.1"
                     /translation="MSRRISPVIPGFGLTLGYTLVYLGLLVLIPLGAMFVHAAQLSWG
                     EFWTIVSAPRVLAALKLSFGTALVAAVINGIIGTLLAWVLVRYTFPGRKIIDAMIDLP
                     FALPTAVAGIALTALYTPNGLLGQLVSELGFKIAFTPLGISLALTFVTLPFVVRTVQP
                     VLADIPREVEEAAACLGAKPLQVFRHVLLPALLPAWLTGFALAFARGVGEYGSVIFIA
                     GNMPMKTEILPLLIMVKLDQYDYTGATSIGVLMLVVSFVLLLLINLLQRRIETP"
     gene            complement(2081..3079)
                     /locus_tag="VIO83_06945"
     CDS             complement(2081..3079)
                     /locus_tag="VIO83_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8331045.1"
                     /translation="MSIRRFALAALAGLSLSAAVQAQTTLLNVSYDPTRELYKEYNAA
                     FNKHWQAQGHEAVSVQQSHGGSGKQARAVIDGLKADVVTLALAGDIDELNKLGKLIPE
                     DWQSRLPQASTPYTSTIVFLVRKGNPKQIKDWDDLVRPGVEVITPNPKTSGGARWNFL
                     AAWAYAEQQYGSEAKAQEFTEQLYKNVPVLDTGARGSTITFVKNNIGDVLLAWENEAF
                     LALQEEGGENFEIVAPSLSILAEPPVAVVDKNVDKHGTREVATAYLNYLYSAEGQRIA
                     AKNFYRPRDSKVAAEFAKQFPNLKLATIDQDFGGWKVAQPKFFNDGGVFDQIYQAQ"
     gene            complement(3280..3462)
                     /gene="oscA"
                     /locus_tag="VIO83_06950"
     CDS             complement(3280..3462)
                     /gene="oscA"
                     /locus_tag="VIO83_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur starvation response protein OscA"
                     /protein_id="HEY8331046.1"
                     /translation="MSATVRSIEGQDEASLLREINNALRGLRFGAVEITVHNGQVVQI
                     ERKEKFRLQSPATRQA"
BASE COUNT          614 a         1088 c         1230 g          599 t
ORIGIN      
        1 acgtcctcgc cgtggaaggc gatgctgccg gcgtccgggg tttccaggcc ggcgatgatg
       61 cgcagcaggg tggtcttgcc gcagccggac gggccgagca gggccaccag ctcgccgctg
      121 tggatatcca ggttgatgtc gttgagggcc tggaaggcgc cgaagcgctt gctgacgttg
      181 cggatctcga tactcatggc ttattcctcg gcagcgttcg acttgaggcg cgccaggcgg
      241 gcctcgctcc actgcttgag cagcaggatg aacagcgcca gggccagcag caggctggcg
      301 acgctgaagg cggcgacgtg gttgtactcg ttgtagagga tctccacgtg cagcggcagg
      361 gtgttggtga cgccgcggat gtggccggaa accaccgata ccgcgccgaa ctcgcccatc
      421 gcccgcgcgg tacacagcac cacgccgtag atcaggcccc acttgatgtt cggtacggtg
      481 acgtgccaga acatctgcca gccgttggcg ccgagcaggc gcgcggcctc ctcttcctgg
      541 gtgccctgct cctgcatcag cgggatcagc tcgcgggcga cgaagggcac ggtgacgaac
      601 agggtggcca gcacgatgcc gggcacggcg aagacgatct gcacgtcgcg ctcgctcagc
      661 cactcgccga agaagccctg ggcaccgaac agcagcacgt agatcagacc ggcgatcacc
      721 ggcgacaccg agaacggcag gtcgatcagg gtgaccaaca ggctcttgcc gcggaattcg
      781 tacttgctca cgcaccaggc ggcggcgacg ccgaacacca ggttgagcgg caccgagatg
      841 gccacggcga gcaaagtcag ctggagcgcg gcgatggcgt ccggctcgac gatcgccgtg
      901 aagaaggtgc ccaggcccag cttcagcgcc tcgctgagca ccacgtagag cggcagcagg
      961 aggaacaggg cgaacaccag ccaggcgctg acgatcagca ggttgcgccc cagcgcatgg
     1021 ccgcggcgcg cggcgttggc atgggcactg gcggaggcag cggttacgga tgacatgacg
     1081 gcttcctcct cacggggttt cgatgcggcg ctgcagcagg ttgatcagca gcagcaggac
     1141 gaaggacacc accagcatca gcacgccgat gctggtggcg ccggtgtagt cgtactggtc
     1201 gagcttgacc atgatcagca gcggcaggat ctcggtcttc atcggcatat tgccggcaat
     1261 aaaaatgacg gatccgtact cgccgacgcc gcgggcgaag gccagggcga agccggtcag
     1321 ccaggccggc agcagcgccg gcagcagcac gtggcggaac acctgcagcg gcttggcgcc
     1381 caggcaggcg gcagcctcct cgacctcgcg cgggatgtcg gccagcaccg gctgcaccgt
     1441 gcgcaccacg aacggcaggg tcacgaaggt cagcgccagg ctgatgccca gtggggtgaa
     1501 ggcgatcttg aagcccagtt cgctgaccag ctggccgagc aggccgttgg gcgtgtacag
     1561 cgcggtcagg gcgatgccgg ccaccgcggt gggcagggcg aacggcaggt cgatcatcgc
     1621 gtcgatgatc ttgcgcccgg ggaaggtgta gcgcaccagc acccaggcca gcagggtgcc
     1681 gatgatgccg ttgatcacgg cggcgaccag ggcggtgccg aaactcagct tgagcgcggc
     1741 cagcacccgc ggcgcggaaa cgatggtcca gaactcgccc cagctgagct gggcggcgtg
     1801 gacgaacatg gcacccagag gaatcagcac caacaggccg aggtacacca gggtgtagcc
     1861 cagcgtcagc ccgaagccgg gtatgacggg ggaaatgcgg cgggacatag gctcctctgg
     1921 atcacatgac gggattcgcc gtgcggcgaa cccgtggatt atgcagcgaa gcgctgcagc
     1981 gcccggaaag acgctgcagc gcaggatttg gccaagtcca gttcggcagg gtgcgccggt
     2041 catgcaccgt ggcgctggtg cgcacagcgc accctacgga tcactgggcc tggtagatct
     2101 ggtcgaacac gccgccgtcg ttgaagaact tcggctgggc gactttccag ccaccgaaat
     2161 cctggtcgat ggtggccagc ttgaggttgg ggaactgctt ggcgaactcg gcagcgacct
     2221 tgctgtcacg cggacggtag aagttcttcg ccgcgatacg ctggccttcc gcgctgtaca
     2281 ggtagttcag gtaggcggtg gccacttcgc gggtgccatg cttgtcgacg ttcttgtcga
     2341 ccaccgccac cggcggctcg gcgaggatcg acagcgacgg cgcgacgatc tcgaagttct
     2401 cgccgccctc ttcctgcagg gcgaggaacg cctcgttttc ccaggccagc agcacgtcac
     2461 cgatattgtt cttgacgaag gtgatggtcg agccacgggc gccggtgtcc agcaccggga
     2521 cgttcttgta cagctgctcg gtgaattcct gcgccttggc ctcgctaccg tactgctgct
     2581 cggcataggc ccaggcagcg aggaagttcc agcgcgcacc gccggaggtc ttcgggttgg
     2641 gggtgatcac ttccacgccc ggcctgacca ggtcgtccca gtccttgatc tgcttggggt
     2701 tgcccttgcg caccaggaac acgatggtcg aggtgtaggg ggtgctggcc tgcggcaggc
     2761 ggctctgcca gtcttccggg atcagcttgc cgagcttgtt gagctcgtcg atgtcgccgg
     2821 ccagggccag ggtcaccacg tcggccttga ggccatcgat caccgcacgg gcctgcttgc
     2881 ccgagccgcc gtgggactgc tgcacgctca cggcctcgtg gccctgggcc tgccagtgct
     2941 tgttgaaggc ggcgttgtat tccttgtaca gctcgcgggt cgggtcatag gacacgttga
     3001 gcagggtggt ctgtgcctgt accgcagcgc tcagggaaag gccggcgaga gcggccaggg
     3061 cgaaacgacg aatggacatg ctgttgctcc ttgctgcgtg cctctctggc acgccaatca
     3121 attcaggggt ggtgaaggtc ttggctggac ttcagcgaca cattcgccag caacaacggt
     3181 ggtagaggtg cgtgctcatg gcgatactcg tgctcatctt gtggttatgg ctcggggtca
     3241 tgttcacgcc ctccggtgat ccagtcgggc tggtcggcgt caggcctggc gcgtggccgg
     3301 cgactgcagc cggaattttt ccttgcgctc gatctgcacc acctggccgt tgtgcacggt
     3361 gatttccacc gcaccgaaac gcaggccgcg cagggcgttg ttgatctcgc gcaggaggct
     3421 ggcttcgtcc tggccttcga tactgcgaac ggtggcgctc atgctgatgc tccttgatcg
     3481 gctcgccacc ggctggaggg atggcgtgga cgcatcttag gcagagcctg a
//
LOCUS       DATCUN010000171         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_270025, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000171 DATCUN010000000
VERSION     DATCUN010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270025"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VIO83_06955"
     CDS             complement(<1..309)
                     /locus_tag="VIO83_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HEY8331047.1"
                     /translation="MLSLFQRKRVPPAAGTPPTSAIETPKGSMRPESAASLLATPRRQ
                     KLLEHIWQRTSLSRRQFATLYLAPLERYAELVQQFPASENHHHAYPGGMLDHGLEIVA
                     Y"
     gene            complement(512..1402)
                     /locus_tag="VIO83_06960"
     CDS             complement(512..1402)
                     /locus_tag="VIO83_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEY8331048.1"
                     /translation="MLLAVAPDVFVGDIFAMKGSTAINLFVRDMPRLSVDIDVIYLPW
                     QTPRDQALQAINQELAAIATRVAPLGVQTRLVRAKDLGDTKLIVENDAQQVKIEVNVV
                     FRGSVLPIERRPLSTRTSDLFGVEFELPVLAPDELYASKLVAALDRQHPRDLFDVWQL
                     YESGDISDGMVECFVIYLAGHNRPPHEVLFGNDKDIAGEYERAFVGMTEVDCSLETLL
                     DARARMRRELPQRLSTAHKQFLSGLARAEPDWSLVQCQHAAQLPALRWKLANLETFRK
                     RRPDDFAAQSAALDTGLGQS"
     gene            complement(1425..2183)
                     /locus_tag="VIO83_06965"
     CDS             complement(1425..2183)
                     /locus_tag="VIO83_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009397193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEY8331049.1"
                     /translation="MGGNSRHQVIKRLQAGLPRGAPFDLATLSQFGVSPQLAAHYADG
                     GWLVRLAHGVYAFPNDEFGVYGALKFLQQRVPGLHVGGKSALALQGVRHNLGSRETLV
                     LWGDGRFALPAWFTSRFPARYVHARLFDWPDTALASKTLTTPPGLPEDLRVTAPERAV
                     LELLYEAGVKQSLEEARNLFDGLRSPREDLLGQLLSCCTSVKAVRLFLTWARETNLVD
                     VDALLEQYPVRTGSNTRWMSRLDDGTLLSLRPHG"
     gene            complement(2446..2724)
                     /locus_tag="VIO83_06970"
     CDS             complement(2446..2724)
                     /locus_tag="VIO83_06970"
                     /inference="COORDINATES: protein motif:HMM:NF025304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8331050.1"
                     /translation="MGKHYSHMSAEERAVLQLERDQGSSLRSIGRRLGRSASTLSREV
                     RLLGMSSYSAHPAGEGYRQRRRNSVRARKLVEGTELHQFVHDRLVLER"
BASE COUNT          450 a          879 c          923 g          501 t
ORIGIN      
        1 ataggcgacg atctccaggc cgtggtccag catgccgccc ggataggcgt ggtggtggtt
       61 ctcggaggcc gggaactgct ggaccagctc ggcgtagcgc tccagtgggg cgaggtagag
      121 cgtggcgaac tgccggcgcg agagcgatgt gcgctgccag atgtgttcca gcagtttctg
      181 ccggcgcggc gtggccagca gcgatgcggc cgactccggc cgcatcgacc ctttcggagt
      241 ttcgatggcg gatgtgggcg gtgtgccggc ggcgggtggc acccttttgc gctggaacag
      301 cgagagcatg gcgaaccccg gatgacgggc tgctcggggc gccttttggc cttttcagga
      361 tagggccttt ccccttggcg cccttccttt gccccttccc agccctttgg cctttgtcgg
      421 aagcctttgg gtatagggcc gctgcttcgc gggtgccacg atgaatgcgg catggggcaa
      481 tcccggcaag tggggcgcag caggatgttc gtcagctctg gcccaagccg gtgtcgaggg
      541 cggcggattg cgctgcgaag tcgtcgggac ggcgcttgcg gaaggtttcg agattggcga
      601 gcttccagcg cagtgccggt agttgcgcgg cgtgctggca ctgcaccagc gaccagtccg
      661 gttctgcgcg cgccagcccg ctcagaaact gcttgtgcgc ggtgctcagt cgctgtggca
      721 gctcgcgccg catcctggcg cgagcgtcga gcagtgtctc cagggagcag tccacttcgg
      781 tcatgccgac aaaggcccgc tcgtactcgc cggcgatgtc cttgtcgttg ccgaacagca
      841 cttcgtgggg cggtcggttg tggcccgcca gatagatcac gaagcactcg accatgccgt
      901 cgctgatgtc gcccgattcg tagagctgcc acacgtcgaa caggtcgcgg gggtgctgcc
      961 gatccagcgc ggccaccagc ttgctggcgt acagctcgtc cggtgccaga accggcagct
     1021 cgaactcgac gccgaacagg tcgctggtcc tggtgctcag cggccgccgc tcgatgggca
     1081 gcacgctgcc gcggaacacg acattgacct cgatctttac ctgctgggcg tcgttctcga
     1141 cgatcagctt ggtgtctccc aggtccttgg cgcgaaccag gcgtgtctgc acgcccagtg
     1201 gcgcaacgcg cgtggcgatg gcagccagct cctggttgat ggcttgcagc gcttggtcgc
     1261 gcggcgtctg ccacgggagg tagatcacgt cgatgtccac cgacaggcgc ggcatgtccc
     1321 gcacgaacag gttgatggcc gtgctgccct tcatggcgaa gatgtcgccc acgaacacgt
     1381 ccggcgcgac ggccagcagc aggcgaaggg tgtccgccta ggtcttatcc atgaggtctc
     1441 aggctcagca aggtgccgtc atcgagccgg ctcatccagc gcgtgttgct gccggtgcga
     1501 accgggtact gctccagcag agcatcgaca tccacgaggt tggtctcgcg cgcccaggtg
     1561 aggaacaggc gcacggcctt cacgctggtg cagcaggaca gcagttgtcc gagcaagtcc
     1621 tcgcgggggg aacgcagtcc atcaaagagg ttgcgagcct cttcgaggct ctgcttgacg
     1681 ccggcttcgt acagcagctc cagaacggca cgctcggggg ctgtcacccg caaatcctcg
     1741 ggcaggccag gcggcgtggt cagggtcttg ctggccagcg ccgtgtccgg ccagtcgaac
     1801 aggcgggcgt gcacgtagcg ggccggaaag cgcgaggtga accaggctgg caacgcgaaa
     1861 cgaccgtcgc cccagagcac cagcgtctcc cggctgccca ggttgtgccg cacgccctgc
     1921 aaggccaggg cgctcttgcc gccgacgtgc aggccgggca cgcgctgttg caggaacttc
     1981 agcgcaccgt agaccccgaa ctcatcgttc gggaaggcat aaacgccatg ggccagacgc
     2041 acgagccacc cgccgtcggc atagtgggcg gcgagctggg gagacacccc gaactggctc
     2101 agggtggcaa ggtcgaacgg cgccccgcgg gggaggcccg cctgcagccg cttgattact
     2161 tggtgcctgg aatttccacc catgctataa aaatagcacg gaattcaatc agatcctgga
     2221 gctttcgcaa gaacgcgcaa ggaaagtagc atcaagctcc agtggccagg cagcaggcgc
     2281 tgttgaaccg cttcgggccg atgcacgatg cgcagttcct caggcaccca ggtgcacttg
     2341 gctgccgtgc ttctatgtag cccccgcttc ggcttgccct ggcgcaaggc gtcgatcagg
     2401 ggctgcttaa tcgtggcgca gtctgctggc aatctgctgc ggagatcagc gctccagcac
     2461 cagacgatcg tgcacgaact gatgcagttc cgtgccttca accagcttgc gggcccgcac
     2521 gctgtttcgg cgtcgctggc ggtagccttc ccccgccggg tgagccgaat acgacgacat
     2581 gccgagcagg cgcacctctc ggctcaaggt cgatgcactg cggcccagcc tgcgcccgat
     2641 gcttcgaagg cttgaccctt gatcccgctc caactgcagt accgcacgct cttccgcact
     2701 catgtgactg tagtgttttc ccatctgcac accttaccct tggcgaggtg ttg
//
LOCUS       DATCUN010000172         3911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_137967, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000172 DATCUN010000000
VERSION     DATCUN010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137967"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VIO83_06975"
     CDS             complement(<1..857)
                     /locus_tag="VIO83_06975"
                     /inference="COORDINATES: protein motif:HMM:NF025124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8331051.1"
                     /translation="MKDQPDLEWKDLQPESAGTAAGRFPHPDTGGSWDDQPIPQVQLA
                     DLDARALADFRQRAASSQRLPGSAAEDDAALVEQLQLFHLKRAAVLLFHAEPEKLVTG
                     AYIRIGHFRRDNGLVEQHAIGGNLFAQLERSLALLEEHLQRDPDGGSEPRAPADYPLP
                     LAALREALVNAIVHKDYASGIPIQIRIHDDRISLWNPARLAQGWSVASLRARHLSRPG
                     NPDIANAFYRAGLIEAWGLGIDKLIAACRAHGIVEPLLRHEQDGLWLEFAFTRALPRH
                     FAASPASRGA"
     gene            complement(1006..2127)
                     /locus_tag="VIO83_06980"
     CDS             complement(1006..2127)
                     /locus_tag="VIO83_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY8331052.1"
                     /translation="MDVFRYLHLMVEQGGSDLFFSVGAPPQLKREGNSLPIGKTPLAP
                     GSVQQLAYQMMNQKQIAEFERDLEMNLAIGLDGAGRFRVNVYYQRGDVAMVVRHIKSQ
                     IPSVGELGLPAKLEELAMLDRGLILVVGAAGSGKSTTLAAMLDLRNRSRGGHIVCIED
                     PIEFIHSHQRAIIDQREVGLDTRSFEQALLNVLRESPDVIMIGEIRDLATMQHALHYS
                     ETGHLCLATLHATNTTQAIERICNFFPQEARQQVLGDLALNLQGIVGQRLVPGREEKR
                     VAAVELLLKTPYIADLIQRDQLDEIKTALGRSQELGLQTFDQHLYSLVQAGRISLDEA
                     LRHADSRTDLTLRAKLARGFHAEDAELKVMRDSELNITL"
     gene            complement(2230..2973)
                     /locus_tag="VIO83_06985"
     CDS             complement(2230..2973)
                     /locus_tag="VIO83_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016489952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8331053.1"
                     /translation="MTLRTALFYFLLGLSTVIWCILSLLIAPWLPQKARYIFIARTWC
                     RFAVWLSRAMLGIDYRLEGLQNIPHQPCVILAKHQSAWETFFLTAHFAPLTQVLKQEL
                     LRIPFYGWAFKLLKPIGIDRSNPKQALKQVAHLGLERLQQGNWLLIFPEGTRIPAGEM
                     GKFSRSGASVAVAAGVPVLPVAHNAGEVWPRDGWQRHPGTVSVVFGPALHAEGEGPRA
                     VAELNQRAETWIGETMQKINGTARANQAD"
     gene            complement(2970..3515)
                     /gene="gmhB"
                     /locus_tag="VIO83_06990"
     CDS             complement(2970..3515)
                     /gene="gmhB"
                     /locus_tag="VIO83_06990"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEY8331054.1"
                     /translation="MKLLILDRDGVINEDSDAYIKSVAEWIPIPGSVEAIARLSRAGW
                     TVAVATNQSGLARGYYDEATLEAMHARLRRLVAEQGGQLGLIVHCPHGPDEGCDCRKP
                     LPGLLQQIAAHYGIELAGRWFVGDSPGDIAAAQAVGCQPVLVRSGKGERTRAKGLPQG
                     VLVFDDLAAVAAHLLAVEADR"
     gene            complement(3706..>3911)
                     /locus_tag="VIO83_06995"
     CDS             complement(3706..>3911)
                     /locus_tag="VIO83_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018927313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331055.1"
                     /translation="EAVAPLAAARNYRAALERLASLRTPVDAFFDSVLVNADDAAVRA
                     NRYALLSQLRGLFLGVADISLLG"
BASE COUNT          565 a         1245 c         1469 g          632 t
ORIGIN      
        1 gcgccacggc tggcggggct tgcagcgaag tgacggggca gcgcgcgggt gaaggcaaac
       61 tccagccaca ggccatcctg ctcgtggcgc agcaggggtt cgacgatgcc atgggcgcgg
      121 caggcggcga tgagtttgtc gatgcccagg ccccaggctt cgatcagccc ggcgcggtag
      181 aaggcgttgg cgatgtccgg gttgccgggg cgcgacaggt ggcgggcgcg caggctggcg
      241 acgctccagc cctgcgccag gcgcgccgga ttccacaggc tgatgcggtc gtcgtgaatg
      301 cggatctgga tcgggatgcc gctggcgtag tccttgtgca cgatggcgtt gaccagcgcc
      361 tcgcgcagcg ccgccagcgg cagcgggtag tcggcgggag cccgtggttc gctgccgcca
      421 tcggggtcgc gctgcaggtg ctcctcgagc agcgccaggc tgcgctcgag ctgggcgaac
      481 aggttgccgc cgatggcatg ctgctcgacc aggccgttat cgcgacggaa gtggccgatg
      541 cggatgtagg cgccggtgac cagcttttcc ggctcggcgt ggaacagcag cacggcggca
      601 cgcttgagat ggaacagctg caactgttcg accagcgcgg catcgtcctc cgccgcgctg
      661 ccgggcaggc gctggctgct ggcggcgcgc tggcggaagt cggccagggc gcgggcatcg
      721 agatcggcca gttgcacctg ggggatcggc tggtcgtccc agctgccacc ggtatccggg
      781 tgcgggaagc ggccggcggc cgtgccggcg gactccggct ggaggtcttt ccactcgagg
      841 tctggctggt ctttcatccg cgattccctg gttgcgcgcg aggagctccc ggcatgttaa
      901 ccaaatgccg cggacggcgg gcaggcgacg gcgtgcattc gttcatgttc cgcgcacgcg
      961 gctgccgccg ggcggcggcc cggcaggcag catgggggac ggcggttaca gggtgatgtt
     1021 cagctcggag tcgcgcatca ccttgagctc ggcgtcctcg gcatggaagc cgcgggccag
     1081 cttggcgcgc agggtcaggt cggtgcgcga gtcggcgtgg cgcagggcct cgtcgaggct
     1141 gatgcgcccg gcctgcacca ggctgtagag gtgctggtcg aaggtctgca ggccgagctc
     1201 ctggctgcgg ccgagggcgg tcttgatctc gtcgagctgg tcgcgctgga tcaggtcggc
     1261 gatgtagggg gtcttcagca gcagctcgac cgcagccacg cgcttctcct cgcggccggg
     1321 caccaggcgc tggccgacga tgccctgcag gttgagggcg aggtcgccga gcacctgctg
     1381 gcgcgcctcc tgggggaaga agttgcagat gcgctcgatg gcctgggtgg tgttggtggc
     1441 gtgcagggtg gccaggcaca ggtggccggt ctcgctgtag tgcagggcgt gctgcatggt
     1501 ggccaggtcg cggatctcgc cgatcatgat cacgtccggc gactcgcgca gcacgttgag
     1561 cagcgcctgc tcgaagctgc gggtgtccag gccgacctcg cgctggtcga tgatcgcgcg
     1621 ctggtggctg tggatgaact cgatcggatc ctcgatgcag acgatatggc cgccgcgcga
     1681 acggttgcgc aggtcgagca tcgccgccag ggtggtcgac ttgcccgagc cggcggcgcc
     1741 gaccaccagg atcaggccgc ggtcgagcat cgccagttct tccagcttgg ccggcaggcc
     1801 gagctcgccg acgctgggga tctggctctt gatgtggcgc accaccatgg ccacgtcgcc
     1861 gcgctggtag tagacgttga cccggaagcg cccggcgccg tccaggccga tggccaggtt
     1921 catctccaga tcgcgttcga actcggcgat ctgcttctgg ttcatcatct ggtaggccag
     1981 ctgctgtacc gatcccggcg ccagcggcgt cttgccgatc ggcaggctgt tgccctcgcg
     2041 cttgagctgc ggtggcgcgc cgacgctgaa gaacaggtcg gagccgccct gttcgaccat
     2101 caggtgcagg taacgaaaca catccatgcc gcttgcctcc gtgaaccggg aagagtggtg
     2161 gcgctgcccg tgccggcgcg aggccgggca gcgcgacccc ttcccggcgc gggaaggggg
     2221 tgcaggcctt cagtctgcct ggttcgcccg cgccgtgccg ttgattttct gcatggtttc
     2281 gccgatccag gtctccgcgc gctggttgag ctcggccacg gcgcgcgggc cttcgccttc
     2341 ggcgtgcagg gccgggccga acaccacgct gaccgtgccg ggatggcgct gccagccgtc
     2401 acgcggccag acttcgccgg cgttgtgcgc caccggcagc accggaacgc cggcggctac
     2461 cgccaccgag gcgccgctgc gcgagaactt gcccatctcg ccggccggaa tgcgcgtgcc
     2521 ttccgggaag atcagcagcc agttgccctg ctgcaggcgt tccaggccga ggtgggccac
     2581 ctgcttgagc gcctgcttgg ggttgctgcg gtcgatgccg atcggcttga gcagcttgaa
     2641 cgcccagccg tagaacggga tgcgcagcag ctcctgcttg agcacctggg tcagcggcgc
     2701 gaagtgggcg gtgaggaaga aggtttccca cgcgctctgg tgcttggcga gaatcacgca
     2761 gggctggtgc gggatgttct gcagtccttc gaggcggtag tcgatgccga gcatcgcccg
     2821 gctcagccac acggcgaagc ggcaccaagt gcgggcgatg aagatgtagc gggccttctg
     2881 cggcagccag ggggcgatca gcaggctgag gatgcaccag atgacggtgc tcaggccgag
     2941 caggaaatag aacagggcgg tacgcagggt catcggtcgg cctccacggc cagcaggtgg
     3001 gcggcgacgg ccgccagatc gtcgaacacc agcacgcctt gcggcaggcc tttggcccgg
     3061 gtgcgttcac ccttgccgct gcgcaccagc accggctggc agcccaccgc ctgggcggcg
     3121 gcgatgtcgc cggggctgtc gccgacgaac cagcggccgg ccagttcgat gccgtagtgc
     3181 gcggcgatct gctgcagcag gccgggcagc ggcttgcggc agtcgcagcc ctcgtccggg
     3241 ccgtgcgggc agtgcacgat caggccgagc tggccgccct gctcggccac cagccggcgc
     3301 aggcgcgcgt gcatagcctc gagggtggcc tcgtcgtaat agccgcgcgc caggcccgac
     3361 tggttggtgg ccaccgccac ggtccagccg gcccggctga ggcgggcgat cgcctccacc
     3421 gaaccgggga tcgggatcca ttcggcgacg gacttgatgt aggcgtcgga gtcctcgttg
     3481 atgaccccgt cgcggtcgag aatcagcagt ttcataaggg cagcttcaag ggcgtagggt
     3541 ggaaaactcg cgcagcgatt ttccaccggg gtagatggtg gacaaggctg cgccgttgtc
     3601 caccctacgc tttggctctg tggataagga gcggaggaaa tatccacagg gtccggcaat
     3661 tcggtgtctg tggataacgg ccgggaccgc tatccacagg ggcggtcagc cgagcagcga
     3721 gatgtccgcc acgccgagga acagcccgcg cagctggctc agcagcgcgt agcggttggc
     3781 gcgcacggcg gcatcgtcgg cgttgaccag cacgctgtcg aagaacgcat ccaccggcgt
     3841 gcgcaggctg gccagacgct ccagggcggc gcggtagttg cgcgcggcag ccagcggggc
     3901 gaccgcttcg g
//
LOCUS       DATCUN010000173         7730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_270266, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000173 DATCUN010000000
VERSION     DATCUN010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270266"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VIO83_07000"
     CDS             complement(<1..252)
                     /locus_tag="VIO83_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HEY8331056.1"
                     /translation="MRDYPTGAQLLESAEALLRAQLLPALPAEQKQNALMIARAMGIA
                     ARQLQAGEQPERTELADLQALLQIAASPAPTSAEALRAQL"
     gene            complement(252..1265)
                     /locus_tag="VIO83_07005"
     CDS             complement(252..1265)
                     /locus_tag="VIO83_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017133617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEY8331057.1"
                     /translation="MNPELSSPLQAFIAQQAGAASVEFSELRLLSGGAIQENWLAIAR
                     IEGGPLAGTQELVIRADSPTGVSVSHGRAQEFALLRAAFAAGVTVPEPLWLCAGREII
                     GRPFFVMRRVAGTATGHQLVKDRSLGGDRSELARRLGAELARIHSIRPPRADLDFLPS
                     YAEAPALHLIQLHRDYLDNHHTPHPLLEWGLRWLERHAPPRGELVLCHRDFRTGNYMV
                     DGNGLTAILDWEFAGWSDPLEDLGWFCARCWRFGADALEAGGIGQREDFYQGYESVSG
                     RPIDRAQVAYWEVMAHMNWAIIAIQQAERHLSGEEDSLLLALTGHIVPELEYEILRMT
                     EGT"
     gene            complement(1262..2428)
                     /locus_tag="VIO83_07010"
     CDS             complement(1262..2428)
                     /locus_tag="VIO83_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017133616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8331058.1"
                     /translation="MDFTLSAEQRDLQQRVRDFIADTIIPYEKDPRCGAHGPSGELRR
                     ELIAKAKAAGLLSAHVSPEYGGLGLDHRSKAVFFEEAGYSPLGPVALNIAAPDEGNMH
                     MLEAIATEEQKERWLRPLAAGEIRSCFCMTEPDPGAGSDPSMLQTTARQVGDEFLISG
                     RKWFITGAEGATLAIIMAKLEDGRATMFLADMNNPGIRIERTMDALDSCFPGGHCVVS
                     FDNLRVPASAILGELGEGFKYAQVRLSPARLTHCMRWLGQARRCHDVASRYAARRMAF
                     GKPLGQHEGVGFMLADNELDMHSARLSIWHTAWVLDQGHLGTSESSMAKVIASEATWR
                     VADRSVQVLGGQGVTGETIVARIFSDMRAFRIYDGPSEVHRWSLAKRIFKRNSA"
     gene            complement(2459..3310)
                     /locus_tag="VIO83_07015"
     CDS             complement(2459..3310)
                     /locus_tag="VIO83_07015"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEY8331059.1"
                     /translation="MQIRTVGVVGAGTMGNGIAQVCAMAGLKVIMVDISEAALARGEQ
                     TINASLDRLVRKESITADAKAAALANLVSTTDKQALAEADLVIEAATEAEALKLAILK
                     DIDAIVRPDALIASNTSSISITKLAAATSRPDKFIGTHFFNPVPVLALVELIRGLQTS
                     DATQAAAEAFVARIGKTSINARNSPGFAVNRILVPMINEAVFVLQEGLASAEDIDAGM
                     KLGCGQPIGPLALADLIGLDTLLAVMDVFYHSFNDSKYRPAPLLREMVEAGYLGRKSG
                     RGFHRYD"
     gene            complement(3358..4545)
                     /locus_tag="VIO83_07020"
     CDS             complement(3358..4545)
                     /locus_tag="VIO83_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HEY8331060.1"
                     /translation="MAQRQIFVVGAARTPIGTFGGSLKDTPLTMLATTACSAALQRSR
                     VDPGEVGHVVLGNVIPTEPRDAYLSRVAAMDSGIPQETPAFNVNRLCGSGLQAIVSAA
                     QAILLGDTDIAIGAGAESMSRGPFILPSLRWGARLGDSGAVDYMNGILHDPWQKTHMG
                     VTAENVAARYGISREQQDALALESQRRAARAIAKGYFQEQIVPVQLQTRKGVVSFASD
                     EHVRGDLSLEQLAAMKPIFQKDNGTVTPGNASGLNDGAAAVVLAEEQRANQLGLQPLA
                     RLVGYAHAGVDPAYMGIGPVPAVRLVLERTRLSIDQIDVIEANEAFAAQACVVIQELG
                     LDPAKVNPNGSGISLGHPVGATGAIITTKAIYELHRTGGRYALVTMCIGGGQGIAAIF
                     ERV"
     gene            5265..5591
                     /locus_tag="VIO83_07025"
     CDS             5265..5591
                     /locus_tag="VIO83_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8331061.1"
                     /translation="MSVIGGLSAISLQASAESADMMQLAEQKQCMTCHALDHDMPKAP
                     SFTSIAKKYPDAALEVLAGKVRNGGEGHWGAAPMPSAGARVEVTDEESRQLVAWILNM
                     RQDSKQ"
     gene            5819..6601
                     /locus_tag="VIO83_07030"
     CDS             5819..6601
                     /locus_tag="VIO83_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8331062.1"
                     /translation="MSKDSVLDCFRLDGRIALVTGASSGLGRHFAKLLAAVGAEVLVA
                     ARRADKLDELVAEIEAAGGKAHALALSVTDQASVVACFDEAARMAGVPDIIVNNAGVT
                     VTKPLLEQGEEDWDSVLDTNLKGGWLVATEGARRMVAADKGGCIVNVASILGERVTGG
                     VAPYAVSKAGLIQATKAMALELARYGIRANVLLPGYIATDLNQDFLGSPAGEKLRARV
                     PSRTFGTPADLDGPMLLLASDAGRHMSGACLAVDGGHLVSTL"
     gene            complement(6736..7623)
                     /locus_tag="VIO83_07035"
     CDS             complement(6736..7623)
                     /locus_tag="VIO83_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8331063.1"
                     /translation="MDLRHLRYFIAVAEEQNIGRAAARLHISQPPLTRQIQQLEDELG
                     VRLFTRTPRGMELTGAGELFLEEARNIRALVDQATERTQRAGQGKLGRLDVAIFGSGI
                     LDTIPKVLHAFREAYPEVKLVLHSMSKGEQIEALRQRRIDAGFNRMLAPLPDLATQLV
                     ATESLFLAVHESSPLAREAVVPFKALAEHPLIVFPTGARPNFVDKVMGLCQQMGFVPE
                     VAQEVGDSVTGVALVAGGFGVCLVPQSASVLTFPGVVYLPISGAPENASVDLSCIYRA
                     DDQSPILDAFLQTIRTFGK"
BASE COUNT         1247 a         2387 c         2708 g         1388 t
ORIGIN      
        1 cagctgtgcg cgcagtgctt cggcgctggt cggggccggg cttgcggcaa tttgcaggag
       61 cgcctgcagg tcggccagct ccgtgcgctc aggctgctcg cccgcctgca gctggcgggc
      121 ggcgatgccc atggcccggg cgatcatcaa ggcattctgt ttctgctcgg caggcagggc
      181 gggcagcagc tgggcgcgca gcagggcttc ggcgctttcc agcagttgtg ctccagtggg
      241 gtagtcgcgc atcaggtgcc ctccgtcatc cgcaggattt cgtattccag ctccgggacg
      301 atgtgtccgg tcagcgccag cagcagcgaa tcctcctcgc cagagagatg gcgctcggcc
      361 tgctggatgg cgatgatcgc ccagttcatg tgcgccatga cttcccagta ggcgacctgc
      421 gcgcggtcga tgggacggcc gctgacgctc tcgtagccct ggtagaagtc ctcgcgctgg
      481 ccgatgccgc cggcttccag cgcgtccgcg ccgaagcgcc agcagcgcgc acagaaccag
      541 ccgaggtcct ccagcggatc gctccagccg gcgaactccc agtcgaggat ggcagtcagg
      601 ccattgccgt cgaccatgta gttgccggtg cgaaaatccc ggtggcagag caccagctcg
      661 ccgcgtggcg gggcgtggcg ctccagccag cgcaggcccc attccagcag cggatgcggg
      721 gtgtggtggt tgtcgaggta gtcgcggtgc agctggatca ggtgcagtgc cggtgcttcg
      781 gcgtagctcg gcaggaagtc gagatcggcg cgcggcgggc ggatcgagtg gatccgcgcc
      841 agctcggcgc ccagccggcg ggccagttcg ctgcggtcac cgcccaggct gcggtccttg
      901 accagctggt gcccggtagc ggtgccggcc acccggcgca tgacgaagaa cggccggccg
      961 atgatttcgc gcccggcgca cagccacagc ggctccggca ccgtgacccc ggcggcgaag
     1021 gccgcgcgca gcagggcgaa ctcctgggcg cggccatggc tgaccgacac cccggtgggg
     1081 gagtcggcgc ggatcaccag ttcctgagtg ccggccagcg gaccaccctc gatccgggcg
     1141 atggccagcc agttctcctg gatggcgccg ccggagagca ggcgcagctc gctgaactcg
     1201 acgctggcgg ccccggcctg ttgggcgatg aaggcctgca gggggctact cagttcggga
     1261 ttcatgcgct gttcctcttg aagatgcgct tggccaggct ccagcgatgg acttccgagg
     1321 ggccgtcgta gatgcggaag gcgcgcatgt cggagaagat ccgcgcgaca atggtctcgc
     1381 cggttacgcc ctggccgccg agcacctgca cgctgcggtc ggcgacccgc caggtggctt
     1441 ccgaggcgat gaccttggcc atgctcgact cgctggtgcc gaggtggccc tggtcgagca
     1501 cccaggcggt gtgccagatg gacaggcggg cgctgtgcat gtccagttcg ttgtcggcga
     1561 gcatgaagcc gacgccctcg tgctggccga gcggcttgcc gaaggccatg cgccgggcgg
     1621 catagcggct ggcgacgtcg tggcagcggc gcgcttggcc caaccagcgc atgcagtggg
     1681 tcaggcgggc aggggagagg cgcacctggg cgtacttgaa gccctcaccc agctcgccaa
     1741 ggatcgcgct ggccggcacc cgcaggttgt cgaagctgac cacgcaatgg ccgccgggga
     1801 agcacgagtc cagtgcgtcc atggtccgct cgatgcggat gcccgggttg ttcatgtcgg
     1861 cgaggaacat ggtcgcccgg ccgtcctcca gcttggccat gatgatcgcc agggtggcgc
     1921 cttccgcgcc ggtgatgaac cacttgcgtc cgctgatcag gaactcgtcg ccgacctggc
     1981 gggcggtggt ctgcagcatc gacggatcgg agccggcgcc cggatcgggc tcggtcatgc
     2041 agaagcacga gcggatctcg ccggcggcca gcgggcgcag ccagcgctcc ttctgctcct
     2101 cggtggcgat cgcctcgagc atgtgcatgt tgccctcgtc gggcgcggcg atgttcagcg
     2161 ccaccggtcc cagcggcgag tagccggcct cctcgaagaa caccgccttg ctgcgatggt
     2221 cgaggcccag gccaccgtat tccggcgaga cgtgcgcgga gagcaggccg gcggccttgg
     2281 ccttggcgat cagctcgcgg cgcagctctc cgctgggacc gtgggcgccg cagcgcgggt
     2341 ccttttcgta ggggatgatg gtgtcggcga tgaagtcgcg cacccgttgc tgcaggtcgc
     2401 gctgttcggc ggaaagggtg aagtccatgg caggctctcg cgggtaggtg gcaaagggtc
     2461 agtcgtagcg gtggaaaccg cgaccgctct tgcggccgag gtagccggcc tcgaccattt
     2521 cccgcagcag cggcgccggg cggtacttgg agtcgttgaa gctgtgatag aacacgtcca
     2581 tcaccgccag cagggtatcc aggccgatca ggtcggccag cgccagcggg ccgatcggtt
     2641 ggccgcagcc cagcttcatg ccggcgtcga tgtcctcggc gctggccagg ccttcctgca
     2701 gcacgaacac cgcctcgttg atcatcggca ccaggatgcg gttgaccgcg aagccggggc
     2761 tgttcctggc gttgatcgag gtcttgccga tcctggcgac gaaggcctcg gcggccgcct
     2821 gggttgcatc gctggtctgc aggccgcgga tcagctcgac cagcgccagt accggcaccg
     2881 gattgaagaa gtgggtgccg atgaacttgt ccggccggct ggtggctgcg gccagcttgg
     2941 tgatggagat cgacgaggtg ttgctggcga tcagcgcgtc gggacggacg atggcgtcga
     3001 tatccttgag gatcgccagc ttgagggctt ccgcctcggt ggcggcctcg atcaccaggt
     3061 cagcctcggc cagggcctgc ttgtcggtgg tgctgaccag gttggccagc gcggccgcct
     3121 tggcgtccgc ggtgatggat tccttgcgca ccagacggtc gaggctggcg ttgatggtct
     3181 gctcgccgcg ggccagtgcg gcctcggaga tgtccaccat gatgaccttg agaccggcca
     3241 tggcacagac ttgggcaatc ccgttgccca tggttccggc gccgacgacg cctacggttc
     3301 ggatctgcat tgtgttgctc ctgtcttgtt cttgttgtca gtgaggcaag tggcgactca
     3361 gacgcgctcg aagatcgcgg cgatgccctg gccgccgcca atgcacatgg tcaccagggc
     3421 atagcggccg ccagtgcggt gcagttcgta gatcgccttg gtggtgatga tcgcgccggt
     3481 cgcgccgacc ggatggccca gcgagatgcc cgagccgttc gggttgacct tggccgggtc
     3541 cagccccagc tcctggatca ccacgcaggc ctgggcggcg aaggcttcgt tggcttcgat
     3601 cacgtcgatc tggtcgatgg acaggcgggt gcgttcgagc accaggcgca cggccggtac
     3661 cgggccgatg cccatgtagg ccgggtcgac cccggcgtgg gcatagccga ccaggcgggc
     3721 cagcggttgc aggccgagct ggttggcgcg ctgctcttcg gccagcacca cggcggcagc
     3781 gccgtcgttg aggccggagg cattgcccgg cgtgacggtg ccgttgtctt tctggaagat
     3841 cggcttcatc gcggccaatt gctcgaggct gaggtcgccg cgcacatgct cgtcgctggc
     3901 gaagctgacc acgcctttgc gcgtctgcag ttgcaccgga acgatctgct cctggaaata
     3961 gcctttggcg atggcccgcg cggcgcggcg ctggctttcc agggcaagcg catcctgctg
     4021 ttcacggctg atgccatagc gcgcggcgac gttttccgcg gtgacaccca tgtgggtctt
     4081 ctgccagggg tcgtggagga tgccgttcat gtagtcgacg gcgccgctat cgccgaggcg
     4141 cgcgccccag cgcaggctgg gcaggatgaa cgggccgcgg ctcatgctct cggctccggc
     4201 gccgatggcg atgtcggtat cgcccagcag gatggcttgg gcggcggaaa cgatcgcctg
     4261 cagacccgat ccgcacaggc ggttgacgtt gaaggcgggg gtttcctggg gaatgccgga
     4321 gtccatggcg gctacgcggc tcagataggc atcgcgtggc tcggtcggga tcacgttgcc
     4381 cagtacgaca tggccgactt cgccggggtc gacccgggag cgttgcaggg ccgcactgca
     4441 ggccgtggtc gccagcatgg tcaggggggt gtccttgagc gaaccgccga aggtgccgat
     4501 gggggtgcgg gctgcaccga ctacgaaaat ctgacgctga gccatggtgt ctccttgttg
     4561 ttatcgggcc aatgggcaca caaggccatc ggcttgagtc gtggagcagg ggagcgaggt
     4621 cttgctgaat tgattaaaaa acagaactga tcgtttgtaa atgggcttgc accgctcgat
     4681 ttgttcttct agattctgac tcgaccgagg cgcaagccgc tgccaggcag gcattgcgcc
     4741 aaggtgagaa cgcatctcct ctttggaagg cgatctcggg accatcccat gcgttcaagg
     4801 tggtcatacc ctggatcgtt gagagacaag gccatgaaaa taaaaagaaa tatgacttgt
     4861 ttttttattg ccggcttgtt cgtgaccctg agttatctgg ccgtgcaatc cccttcgttg
     4921 ccgtccgtcg ccaacgagac agcgatcatc acgcatgggg atgccgtgca taaccccata
     4981 gtcatcaagg gctggccttc gtttttctag acgcttgcag cccgatcagt cgcgcgcgct
     5041 gcaagacctg cgacatcaag gacccgacgc agagcatcac ctgggtggcc ccggaacacc
     5101 ctggcgggcc gtgctaatcc ggtatgcagg tcatgcggtc atacaggctc ttcaagcctc
     5161 cagcgaggct gaacagaggt gccggatagg catttttcat catgctgtgc caataaatga
     5221 aaacaatata tgaggctgat gatatgaaaa aaacaattat ggcaatgtcg gtgattggcg
     5281 gtctttccgc gatttccctg caggcatcgg cggagtcggc ggacatgatg cagctggccg
     5341 agcagaagca gtgcatgacg tgccatgcgc tcgaccacga tatgcccaag gcgccgtcgt
     5401 tcacgagcat tgccaagaag tatccggacg ccgcgctgga agtgctggca ggcaaggtgc
     5461 gtaacggcgg agaagggcac tggggtgcgg cgccgatgcc ttcggcgggg gcccgcgtcg
     5521 aagtgactga cgaggaatcc cgccagctgg tggcctggat cctcaacatg cggcaggaca
     5581 gcaagcagta aaccgcaacc ttcgaggtct ttgcgcaaac tcccctgatg cagatcgggg
     5641 gatccaaaga ctctccggca cagcaccgag cgcgcagagc cgcgtccctt ccaatatctg
     5701 cgacatccac acgcgggtac atgactgccg tgcccaggac gtggtgctgg cgtatggcgg
     5761 gcaatatcct cgctcagtgc ttgctggttg ggtcgaccgc tatccgatgg ggtagagcat
     5821 gagtaaggat tccgttctcg actgttttcg cctggacggg cgcattgccc tggtgaccgg
     5881 tgcctccagt ggcctcgggc ggcacttcgc caagctgctg gctgcggtcg gtgccgaggt
     5941 gctggtcgcc gcccggcgcg ccgacaagct tgacgaactg gtggcagaaa tcgaggctgc
     6001 cggcggcaag gcgcacgcat tggcgctcag cgtcaccgat caggccagtg tcgtcgcctg
     6061 tttcgacgag gcggccagga tggcgggggt gcccgacatc atcgtcaaca atgccggcgt
     6121 cacggtgacc aagcccttgc tcgagcaggg cgaagaagac tgggacagtg tgctcgacac
     6181 caatcttaaa ggcggctggc tggtcgccac cgagggcgcg cggcgcatgg tggcggcgga
     6241 caagggtggc tgcatcgtca acgtggcgtc gatcctgggc gagcgggtga cgggaggcgt
     6301 ggcgccttac gcggtctcga aggccggtct gatccaggcc accaaggcga tggcgcttga
     6361 gctcgcgcgc tacggcatcc gcgccaatgt gctgctgcca ggctacatcg ctaccgatct
     6421 caaccaggac ttcctcggca gccctgcggg cgaaaagctg cgggcgcgag tgcccagtcg
     6481 tactttcggg acgcctgcgg acctggatgg ccccatgctg ctgctggcct ccgatgccgg
     6541 gcgtcacatg tcgggtgcct gcctcgccgt cgacggcggc catctcgtca gcaccctgta
     6601 ggtggccagc agggacgaag caccagctct cgagcagtcc attactgcgg ccgcgttggg
     6661 ctcgtctgcg gttacccggt gcttcgactg cagcgttggc gccggcctat tgccggcatt
     6721 gcattggggc acggatcact tgccgaatgt cctgatcgtc tgcaaaaagg catcgaggat
     6781 cggcgactgg tcgtcggcgc ggtagatgca gctcagatcg acgctggcgt tttcgggcgc
     6841 gccgctgatc ggcaggtata ccactccggg gaaggtcagt accgaggctg actgcggcac
     6901 caggcacacg ccgaaaccgc ccgccaccag ggcgacgccg gtcacggagt cgcccacctc
     6961 ctgcgccacc tccggcacga agcccatctg ctggcagagg cccatcacct tgtcgacgaa
     7021 gttgggccgc gcgccggtgg ggaacacgat cagcggatgc tcggccagcg ccttgaacgg
     7081 cacgacggcc tcgcgggcga ggggactgct ctcgtggacc gcgaggaaaa gcgattcggt
     7141 ggcgacgagc tgagtggcca ggtctggcag cggcgcgagc atgcggttga agcccgcgtc
     7201 gatccggcgc tggcgcaagg cctcgatctg ctcgcccttg ctcatgctgt gcaggacgag
     7261 cttgacctcg ggataggcct ccctgaaggc gtgcagcacc ttcggaatgg tgtcgagaat
     7321 ccccgagccg aagatggcga cgtcgagccg gccgagcttg ccctggccgg cccgctgggt
     7381 ccgctcggtg gcctgatcga ccagcgcccg gatgttgcgc gcttcctcga ggaacagctc
     7441 gcctgctccg gtcagctcca tgccgcgcgg ggtgcgggta aacagccgca cgcccagttc
     7501 atcctccagt tgctggatct gccgggtcag gggtggttgc gagatgtgca ggcgggctgc
     7561 ggcccgcccg atgttctgct cttcggcaac ggcgatgaag tagcggagat gacgcagatc
     7621 catgctggac gaccttgtgg gaggaaactg gtcgcttgat acctttgcgg tagcaagatg
     7681 acgtttttat ggtattggac gctaagccat tcgggcaata gatttctggt
//
LOCUS       DATCUN010000174         5571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_195231, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000174 DATCUN010000000
VERSION     DATCUN010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195231"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VIO83_07040"
     CDS             <1..717
                     /locus_tag="VIO83_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8331064.1"
                     /translation="GTPDFDSIMPVLHAFQDKLDLTAFEFFSDKALAKVLGRGDVPAP
                     FETDCPYYALLEFEASSEEVSNAALELFEHCVEQGWVLDGVMSQSEQQLQNLWKLREY
                     ISETISHWTPYKNDISVTVSKVPAFLKEIDAIVGEHYPDFEIVWFGHIGDGNLHLNIL
                     KPDAMAKEEFFAKCATVNKWVFETVEKYNGSISAEHGVGMTKRDYLGYSRSPEEIACM
                     KAIKAVFDPNGIMNPGKIFA"
     gene            820..1485
                     /locus_tag="VIO83_07045"
     CDS             820..1485
                     /locus_tag="VIO83_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014595619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8331065.1"
                     /translation="MSYQHQYADRTRIHFPVGKLVCVGRNYAEHAKELNNPVPSEPLL
                     FIKPATAAVALEGGFAIPQDRGSVHYEAEIAVLIGKPLSTRPNDEEIRDAISGFGVAL
                     DLTLRDVQSRLKDKGHPWEIAKAFDGACPLSPFVPGDAVEDLADIGVRLSINDELRQD
                     GNSRDMLTPILELIRYISGHFSLQPGDVVLTGTPAGVGALASGDRLVVELPELCRFES
                     HVL"
     gene            1605..2519
                     /locus_tag="VIO83_07050"
     CDS             1605..2519
                     /locus_tag="VIO83_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HEY8331066.1"
                     /translation="MPPRTARRFLLPALLLSIGAGALSASHFMHWGAQLELYWREQAV
                     APAARAAGIWLPDYRLARETSLPGLEQDETSGLTWNPATDTLFTVTGKRPKLVELSRD
                     GQVLRSIELVGFSDTEAVEALDDGRLAIVDERHHLLAVFRLEPGVQRLDLTDTTRYDL
                     GFADAGNKGFEGLAWNPRSRRLLLAKERDPLGLFSLPLPVAADAGVTLEALPSANLPV
                     RDLSSLTVDPRSGRTLLLSDESRLLLELDEQGRPFSFLSLFGGRNGLGDGIQQAEGVA
                     MDAQGNIYVVGEPNRFYVFSRQPGSTPR"
     gene            complement(2524..2865)
                     /locus_tag="VIO83_07055"
     CDS             complement(2524..2865)
                     /locus_tag="VIO83_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014595622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEY8331067.1"
                     /translation="MSGKFRLKKASNGQFQFNLEASNGQPILSSEQYKAKDSALNGIE
                     SVRKNAVREGAFEPLTAKDGRPYFVLKASNGQVIGQSQMYASAASCNQGMESVKKHAP
                     EALLVDETLAG"
     gene            complement(3035..3706)
                     /gene="rpiA"
                     /locus_tag="VIO83_07060"
     CDS             complement(3035..3706)
                     /gene="rpiA"
                     /locus_tag="VIO83_07060"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEY8331068.1"
                     /translation="MNQDQLKQAVAQAAVDFIHPRLESRSIVGVGTGSTANCFIDALA
                     KIRQDFDGAVASSDATAARLKSHGIPVYDLNSVPELEFYVDGADETNARLELIKGGGG
                     ALTREKIVAAVAKTFICIADGSKLVERLGAYPLPVEVVPMARSFVARELVKLGGDPEY
                     REGFVTDNGNIILDVHNLLIGDAVALEQRINNIVGVVTNGLFAARPADLLLLGTNEGV
                     RRLER"
     gene            3796..5370
                     /gene="ilvA"
                     /locus_tag="VIO83_07065"
     CDS             3796..5370
                     /gene="ilvA"
                     /locus_tag="VIO83_07065"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEY8331069.1"
                     /translation="MGGGESRLGPIPPTPSANSPMLEQYVKKILTSPVYDVAVETPLQ
                     PARSLSERLNNQILLKREDLQPVFSFKIRGAYNKVAQLSEEEKARGVIAASAGNHAQG
                     LALTAKKLGIKAIIVMPKSTPDIKVQAVRARGARVVLHGDAFPEALAHCLKLVEEKGY
                     TFVHPYDDPDVIAGQGTIAMEILRQQPGRLDAIFVPVGGGGLIAGIAAYVKYLRPDIK
                     VIGVEPEESDCLRAALAADERVILSQVGLFADGVAVAQIGAEPFRICRAFVDEVVTVT
                     TDEICAAMKDIYDDTRSITEPAGALGVAGIKRYVQRRGVSGQTLVAIDSGANVNFDRL
                     RYVAERAELGEQREAIIAVTIPEKPGSFKAFCQAIGKRAITEFNYRYKSPAEAQIFVG
                     VQTHPENDPREQLVAMLREQGFPVLDLTDSELAKLHIRHMVGGHAPGAENERVYRFEF
                     PERPGALFNFLNKLGGRWNISMFHYRNHGAADGRVLVGLQVPEDELALLTEALDGIGY
                     PYWDESNDPAYRLFLG"
     gene            5446..>5571
                     /locus_tag="VIO83_07070"
     CDS             5446..>5571
                     /locus_tag="VIO83_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019093431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8331070.1"
                     /translation="MRILLVGAGGFIGRHLHVALRAAGHQVLATARQPDPGASGDW"
BASE COUNT          929 a         1955 c         1815 g          872 t
ORIGIN      
        1 ggcaccccgg atttcgattc gatcatgccg gtgctgcacg ccttccagga caagcttgac
       61 ctcactgcct tcgagttctt ctccgacaag gcgctggcca aggtgctggg ccgcggcgac
      121 gtgccggcgc cgttcgagac cgactgcccg tactacgcgc tgctcgaatt cgaagccagc
      181 agcgaggagg tgtccaacgc cgccctggag ctgttcgagc actgcgtcga gcagggctgg
      241 gtgctcgacg gcgtgatgag ccagagcgag cagcagctgc agaacctgtg gaagctgcgc
      301 gagtacatct ccgagaccat ctcgcactgg accccgtaca agaacgacat ctcggtcacc
      361 gtgtcgaagg tgccggcgtt cctcaaggaa atcgacgcca tcgtcggcga acactacccg
      421 gacttcgaga tcgtctggtt cggccacatc ggcgacggca acctgcacct gaacatcctc
      481 aagcccgacg ccatggccaa ggaagagttc ttcgccaagt gcgccacggt gaacaagtgg
      541 gtgttcgaga ccgtcgagaa gtacaacggc tcgatctccg ccgagcacgg tgtgggcatg
      601 accaagcgcg actacctggg ctacagccgc tcgccggaag aaatcgcctg catgaaggcg
      661 atcaaggcag tgttcgatcc caacgggatc atgaaccccg gcaagatctt cgcctgactc
      721 gcgtagggtg gaaaaccgct tagcgttttc caccttggcc gcgaacggtg gaaaaggcct
      781 ccggccgttt tccaccctac ggattatcag gagtctttca tgagctacca gcaccagtat
      841 gccgaccgca cccgcatcca cttcccggtg ggcaagctgg tctgcgtcgg ccgcaactac
      901 gccgaacacg ccaaggaact gaacaacccg gtgcccagcg agccgctgct gttcatcaag
      961 ccggccaccg ccgccgtggc gctggagggc ggcttcgcca ttccgcagga tcgcggcagc
     1021 gtgcactacg aggcggagat cgccgtgctg atcggcaagc cgctgtcgac ccggccgaac
     1081 gacgaggaaa tccgcgatgc catcagcggc ttcggcgtgg cgctggacct gaccctgcgc
     1141 gacgtgcaga gccgcctcaa ggacaagggc cacccgtggg aaatcgccaa ggccttcgac
     1201 ggcgcctgcc cgctgtcgcc gttcgtcccc ggcgatgccg tggaggacct ggccgacatc
     1261 ggcgtgcgcc tgagcatcaa cgacgagctg cgccaggacg gcaacagccg cgacatgctg
     1321 acgccgatcc tcgagctgat ccgctacatc agcggccact tctcgctgca gccgggcgac
     1381 gtggtgctga ccggcacccc ggccggcgtc ggcgcgctgg ccagcggcga ccgcctggtg
     1441 gtcgagctgc cggagctgtg ccgcttcgag agccacgtgc tctgagccac gccacgagcc
     1501 gggcgagtac cggctcgtgg ctcccctctc cggggaaaat tcacattgtt ttcaaccggc
     1561 tgcaggcatg ctgtggcacc cgccatcctg agagcctgtc gcccatgccc cctcgcaccg
     1621 cccgccgctt cctgctgccc gccctcctgc tgtcgatcgg cgccggagca ctgagcgcca
     1681 gccacttcat gcactggggt gcgcagctgg aactgtactg gcgcgagcag gcggtggccc
     1741 ccgcggcgcg cgccgccggc atctggctgc cggactaccg cctggcgcgg gaaaccagcc
     1801 tgcccggcct ggaacaggac gaaacctccg gactgacctg gaatccggcg accgacaccc
     1861 tgttcaccgt caccggcaag cgtcccaagc tggtcgaatt gagccgtgac ggccaggtgc
     1921 tgcgcagcat cgaactggtc ggtttctccg acaccgaggc ggtcgaagcg ctggacgacg
     1981 gccgcctggc catcgtcgac gaacgccacc acctgctggc ggtgttccgc ctggagccgg
     2041 gcgtgcagcg cctcgatctg acggacacca cccgctacga tctcggcttt gccgacgccg
     2101 gcaacaaggg cttcgagggt ctggcctgga atccgcgcag ccgacgcctg ctgctggcca
     2161 aggagcgcga cccgctgggc ctgttcagcc tgccgcttcc cgtcgcggcg gacgcgggcg
     2221 tgacgctcga ggcgctgccc agcgccaacc tgccggtgcg cgacctgtcc tcgctgaccg
     2281 tcgacccgcg cagcggccgc accctgctgc tctccgacga gtcgcgcctg ctgctggagc
     2341 tggacgagca gggcaggccc ttcagcttcc tcagcctgtt cggcggccgc aacggcctgg
     2401 gcgacggcat ccagcaggcc gaaggcgtgg ccatggatgc gcagggcaac atctacgtgg
     2461 tcggcgaacc caatcgcttc tacgtgttca gccgccagcc gggcagcacg ccgcgctgag
     2521 gtcctagccg gccagcgtct cgtccaccag cagcgcctcg ggcgcgtgct tcttgaccga
     2581 ctccatgccc tggttgcagc tggcggcgct ggcgtacatc tggctctggc cgatcacctg
     2641 gccgttgctg gccttgagca cgaagtacgg ccgcccgtcc ttggcggtca gcggctcgaa
     2701 ggcgccctcg cgcacggcat tcttgcgcac cgactcgatg ccgttgagtg ccgaatcctt
     2761 ggccttgtac tgctcgctgc tcaggatcgg ctggccgttg ctggcctcca ggttgaactg
     2821 gaactggccg ttgctggcct tcttcagacg aaacttgccg gacatggtct acctcctcgg
     2881 ttggcggatc gcttaccgat aaaactagac gctattagcg cgggctgccg ggcaagctgg
     2941 cggctcgccg gcaaaagcgc gaacccgctc cccgcaaagc aagaaggccg gcgcacccca
     3001 gggtggccgg ccttccggta atgcccgcgg ggactcagcg ctcgaggcgg cgcacaccct
     3061 cgttggtgcc gagcagcaac aggtcggccg ggcgggcggc gaacagaccg ttggtgacca
     3121 cgccgacgat gttgttgatg cgctgctcca gtgccaccgc atcgccgatc agcaggttgt
     3181 gcacgtcgag gatgatgttg ccgttgtcgg tgacgaagcc ttcgcggtac tcggggtcgc
     3241 cgcccagctt gaccagctcg cgggccacga agctgcgcgc catcggcacc acttccaccg
     3301 gcagcgggta ggcaccgaga cgctcgacca gcttgctgcc gtcggcaatg cagatgaagg
     3361 tcttggccac ggccgcgacg atcttctcgc gggtcagcgc gccgccaccg cccttgatca
     3421 gctccaggcg cgcgttggtc tcgtcggcac cgtcgacgta gaactccagc tcggggacgc
     3481 tgttgaggtc gtataccgga atgccgtggc tcttcaggcg ggcggcggtg gcatccgagc
     3541 tggccactgc gccgtcgaag tcctgacgga tcttcgccag ggcgtcgatg aagcagttgg
     3601 cggtggagcc ggtgccgacg ccgacgatgc tgcgcgactc gaggcgcgga tggatgaaat
     3661 cgacagcggc ctgggccacc gcctgcttga gctgatcctg gttcatggcg gattccgcga
     3721 agagaaaaat cgaccggcat tataacgtca agacggcgcg gatcggcgcc ccacgcgggt
     3781 ttttgccggt ggccgatggg cggtggcgag agtagactcg ggcccattcc gcctaccccg
     3841 tctgcgaact ctccgatgct cgaacagtac gtgaagaaga tcctcacctc gcccgtctac
     3901 gatgtggcgg tggaaacccc cctgcaaccg gcgcgcagcc tgtccgagcg cctgaacaac
     3961 cagatcctgc tcaagcgcga ggatctgcag ccggtgttct ccttcaagat ccgcggcgcc
     4021 tacaacaagg tcgcccagct cagcgaggag gaaaaggccc gcggggtgat cgccgcctcc
     4081 gccggcaacc acgcccaggg cctggcgctg accgccaaga agctcggcat caaggcgatc
     4141 atcgtcatgc ccaagagcac gccggacatc aaggtgcagg cggtgcgcgc ccgcggcgcc
     4201 cgcgtggtgc tgcacggcga cgccttcccc gaggcgctgg cgcactgcct caagctggtc
     4261 gaggagaagg gctacacctt cgtgcatccc tacgacgacc ccgacgtgat cgccggccag
     4321 ggcaccatcg ccatggagat cctccgccag cagccgggtc gcctcgacgc aatcttcgtg
     4381 ccggtcggcg gcggcggcct gatcgccggc atcgccgcct acgtcaagta cctgcgcccg
     4441 gacatcaagg tgatcggcgt cgagccggag gaatccgact gcctgcgcgc cgccctggcc
     4501 gccgacgagc gggtgatcct cagccaggtc ggcctgttcg ccgacggcgt ggcggtggcg
     4561 cagatcggcg ccgagccgtt ccgcatctgc cgcgccttcg tcgacgaggt ggtcaccgtg
     4621 accaccgacg agatctgtgc ggcgatgaag gacatctacg acgacacccg ctcgatcacc
     4681 gagccggctg gcgccctcgg cgtggccggg atcaagcgct acgtgcagcg ccgcggcgtc
     4741 agcgggcaga ccctggtggc catcgactcc ggcgccaacg tcaacttcga ccgcctgcgc
     4801 tatgtcgccg agcgcgccga gctgggcgag cagcgcgagg cgatcatcgc cgtgaccatc
     4861 ccggagaagc cgggcagctt caaggcgttc tgccaggcca tcggcaagcg cgcgatcacc
     4921 gagttcaact accgctacaa gtcgccggcc gaggcgcaga tcttcgtcgg cgtgcagacc
     4981 catccggaga acgacccgcg cgagcagctg gtggcgatgc tgcgcgaaca gggcttcccg
     5041 gtgctcgacc tgaccgacag cgagctggcc aagctgcaca tccgccacat ggtcggcggc
     5101 cacgcgccgg gcgcggagaa cgagcgggtg taccgcttcg aattccccga gcgccccggc
     5161 gcgctgttca acttcctcaa caagctcggc gggcgctgga acatctcgat gttccactac
     5221 cgcaaccacg gcgccgccga cggccgcgtg ctggtcggcc tgcaggtgcc ggaggacgag
     5281 ctggcgctgc tcaccgaagc gctggacggc atcggctatc cgtactggga cgagagcaac
     5341 gacccggcct accggctgtt cctcggctga ccccggccgc accgatcaca gggaggtgat
     5401 cgccatgccc cacccgctcc ccctgccgac ccggagggcg cgctgatgcg catcctgctg
     5461 gtcggcgccg gcggcttcat cggccgccac ctgcatgtcg cgctgcgtgc ggccggccac
     5521 caggttctcg ccacggcacg ccagcccgac cccggcgcca gcggcgattg g
//
LOCUS       DATCUN010000175         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_138143, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000175 DATCUN010000000
VERSION     DATCUN010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138143"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..364)
                     /locus_tag="VIO83_07075"
     CDS             complement(137..364)
                     /locus_tag="VIO83_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331071.1"
                     /translation="MLEATLAQLEGLVAELLQQNQTLTASCQQLEQQLQQAREENDAL
                     QMAALEQEEQQGATLARLQALVQRAGASSAA"
     gene            724..1422
                     /locus_tag="VIO83_07080"
     CDS             724..1422
                     /locus_tag="VIO83_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8331072.1"
                     /translation="MGAQWKAKPKEAAANARGKIFGKLVKEIMIAARNGADPDMNPKL
                     RLAVHQAKKASMPKETLERAIKKGAGLSGEVVNYERTVYEGFAPHQVPVIVECLTDNV
                     NRTVAEVRVLFRKGQMGSSGSVSWDFDHLGMIEATPEGDADPELAAIEAGAQDFEPAE
                     EGATLFYTDPTDLDAVCKALPEFGFTVQSAQLGYKAKNPVSLSGTDLEEVEAFLEALD
                     GHDDVQNVYVGLAG"
     gene            complement(1489..2040)
                     /locus_tag="VIO83_07085"
     CDS             complement(1489..2040)
                     /locus_tag="VIO83_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331073.1"
                     /translation="MNKMSIALALAGATLLGGCASSNYAPVESPTRLQVAFADPAWDG
                     QTVPAGQRCRWAGGHGSTPRLLVSNIPSGANALVVEFSDRSYFLMDHGGHGKIGVWLS
                     GAPSSITIPSVAGESLDLPEGMFVEKEHQGWLRGNGGAYLPPCSGGRGHRYYATVKAV
                     YKAKAAGEQSRLLGEQIIEMGKY"
     gene            complement(2219..>2617)
                     /locus_tag="VIO83_07090"
     CDS             complement(2219..>2617)
                     /locus_tag="VIO83_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2076 family protein"
                     /protein_id="HEY8331074.1"
                     /translation="PATTPAAVQPARSGWHEPAASNQNGFGQASRFGQQPGFGQRGAH
                     APQSGGFLSGALQTAAGVAGGMVLGNMLMDMFQGDEAEKVAEQAPAEPAEQPAAMEEP
                     VAPEENYADSGFFGGGDEGGFFGGDDEDFV"
BASE COUNT          459 a          895 c          841 g          422 t
ORIGIN      
        1 ctgaacgccg cgatcaacat taaagccgcc gggctggcgg tgttagccct tggagagaac
       61 gtaagtggca tcggtcgtgt accgctgtcc tgatctcgat gaattgggaa tctccgtcct
      121 tcagggcgga gagcagtcaa gcggcgctac tggcaccggc acgctgcact agtgcctgca
      181 ggcgggccag ggtggcgccc tgctgctctt cctgctcgag cgcggccatt tgcagggcgt
      241 cattctcttc cctggcctgt tgcagctgtt gctcgagctg ctggcagctg gcggtcaggg
      301 tctggttctg ttgcagcagt tctgccacaa ggccttcaag ttgcgcgagg gttgcttcaa
      361 gcatgggaac tgtccggagc tcgtgaaagg ggcgcgaaga atagataaaa gcccccgccc
      421 ctacaaccac gcaccgccca gcgttgcagc ccggcctcgc gcccaggatg aaccgcacat
      481 acctgatagc aataccggca ctgccacctc atgccccggg aagcaaccgc gctcagcccc
      541 gggagaccct gagcggtcac ccgccagggt gccgcctgat cgcgggttca cgccaaattg
      601 gatatctcca tccgccccgg cttgattccc gacaggtgcc gccaggccgc caagtcgcta
      661 gaatcgccct cttcccgttc ccgggcccgc ccgggactca accgtcgcag cgagagaacc
      721 gatatgggcg cccagtggaa agccaaaccg aaagaagccg ccgccaatgc caggggcaag
      781 atcttcggca agctggtgaa ggaaatcatg attgccgcgc gcaacggcgc cgacccggac
      841 atgaacccca agctgcgtct ggcggtgcat caggccaaga aggcgtcgat gcccaaggag
      901 accctggagc gcgcgatcaa gaagggcgcg gggctgtccg gcgaggtggt caactacgag
      961 cgcaccgtct atgaaggctt cgccccgcac caggtgccgg tgatcgtcga gtgcctgacc
     1021 gacaacgtga accgtaccgt ggcggaagtc cgcgtgctgt tccgcaaggg tcagatgggc
     1081 agttccggct cggtgagctg ggacttcgac cacctgggca tgatcgaagc gactccggaa
     1141 ggcgacgccg atccggaact ggccgccatc gaagccggtg cccaggactt cgagccggcc
     1201 gaggaaggcg ccaccctgtt ctacaccgac ccgaccgacc tggacgcggt gtgcaaggcg
     1261 ctgcccgagt tcggcttcac cgtgcaatcg gcccagctgg gctacaaggc caagaatccg
     1321 gtcagcctgt ccggcactga cctcgaggaa gtcgaagcct tcctcgaggc cctcgacggc
     1381 cacgacgacg tgcagaacgt ctacgtcggc ctggctggct gagttcagcc cacagccaga
     1441 acagggaccc ggggcgtgtc gccatgcgta gacacgccct gccccgcctc agtatttgcc
     1501 catctcgatg atttgctcgc ccagcaggcg ggactgctcg ccagcggcct tggccttgta
     1561 cacggccttc accgtggcgt aatagcggtg tccgcgccct cccgagcagg gcggcaggta
     1621 ggcgccaccg ttgccgcgca gccagccctg gtgttccttt tcgacgaaca tgccctctgg
     1681 caggtcgagg ctctcgcccg ccaccgacgg aatggtgatg ctcgacggcg cgccggacag
     1741 ccacaccccg atcttgccgt ggccaccgtg gtccatcagg aagtagctgc gatcgctgaa
     1801 ctcgacgacg agcgcgttgg cgccactcgg gatgttgctc accagtagcc gtggcgtcga
     1861 accatggccg ccagcccagc ggcagcgctg cccggcgggg acggtctggc catcccaggc
     1921 tgggtcggca aaggccacct gcagccgggt tgggctttcc accggcgcat agttcgatga
     1981 cgcgcaacca ccgagcaggg tcgccccggc caaggccagc gcgatcgaca tcttgttcat
     2041 tggttttcct caatgctcgc cccgaacgat cggtgcaggc gctgcggtgc ggggcagtca
     2101 ggcagcgcga cggcgcatgc tgccatgccg gcccgggaca gctccagagc cggacatgca
     2161 cattgcccgc ccgcacctgt caggcgcggg cgcagtctct caaggaacgc agcagcaatc
     2221 agacgaagtc ttcgtcgtcg ccaccgaaga agccaccttc atcgccaccg ccgaagaaac
     2281 cgctgtcggc atagttttct tccggggcga ccggctcttc catggccgcc ggctgttctg
     2341 ccggttcggc cggtgcctgc tcggcgacct tctccgcctc gtcgccctgg aacatgtcca
     2401 tcagcatgtt gcccagcacc ataccgccgg ccacgccggc agcggtctgc agcgcgccgg
     2461 agaggaagcc gccggactgc ggggcatgcg cgccgcgctg gccgaaaccg ggctgctggc
     2521 cgaagcgcga cgcctgaccg aagccgttct ggttgctggc ggccggctca tgccagcccg
     2581 agcgtgccgg ctgcaccgcc gccggggtag tggcggg
//
LOCUS       DATCUN010000176         5913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_252125, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000176 DATCUN010000000
VERSION     DATCUN010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252125"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VIO83_07095"
     CDS             complement(<1..1175)
                     /locus_tag="VIO83_07095"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019408015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEY8331075.1"
                     /translation="MSDHASSGHGNRALALATLAIHGGQSPDPSTGAVMPPIYATSTY
                     AQSSPGEHQGFEYSRTHNPTRFAYERCVASLEGGSRGFAFASGMAASSTVMELLDAGS
                     HVVAMDDIYGGTFRLFERVRKRTANLQFSFVDLTDLAAFEASITPQTKMVWIETPTNP
                     MLKIVDIAAVSAIAKRHGLVVVVDNTFASPMLQRPLELGADLVLHSATKYLNGHSDMV
                     GGMVVVGDNAELAEQMAFLQNSIGGVQGPFDSFLALRGLKTLPLRMKAHCANALALAQ
                     WLETHPAVEKVIYPGLASHPQHELATRQMNGYGGIVSIVLKGGFEAAKRFCEKTELFT
                     LAESLGGVESLVNHPAVMTHASIPVERRAQLGISDALVRLSVGVEELGDLQVDLEGAL
                     "
     gene            complement(1306..2676)
                     /locus_tag="VIO83_07100"
     CDS             complement(1306..2676)
                     /locus_tag="VIO83_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY8331076.1"
                     /translation="MAIHSSVLDLIGHTPIVKAQRLDTGVCELYLKLESANPGGSIKD
                     RIGLSMIEAAEKRGELTPGATLVEGTAGNTGLGLALVAQQKGYKLILVVPDKMSREKI
                     FNLKAMGAEVRLTRSDVAKGHPEYYQDMAKRVAEETPGAYFINQFGNPDNPAAHEFGT
                     GPEILEQMGGDLDAIVFGCGSSGTMTGLSRAFASASPKTELVLADPVGSILAEYINDG
                     VLNDKSGSWLVEGIGEDFLPSIADFSRVTKAYAITDAESFHTARELLGKEGILGGSST
                     GTLLAAALKYCKEQTTPKKVLVLVCDTGNKYLSKMYNDYWMLDNGFLERPQSGDLRDL
                     ILRPYGQRDTVVIGPNDLLTTAYQRMKLYDVSQLPVMDGDQLVGIVDESDVLLHVYGD
                     EARFRDPVATAMVSKLDRLDVKSPIEALLPVFDRGQVAIVMDGGTFLGLITRIDLLNY
                     LRRRVQ"
     gene            complement(2878..3096)
                     /locus_tag="VIO83_07105"
     CDS             complement(2878..3096)
                     /locus_tag="VIO83_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEY8331077.1"
                     /translation="MFEGQPQSEIDARRKRDPEFKALYERHQKLNKKCMDAQLGVLPI
                     DDLTLGQMKREKLLAKQKLERMYDSPMH"
     gene            complement(3491..4402)
                     /locus_tag="VIO83_07110"
     CDS             complement(3491..4402)
                     /locus_tag="VIO83_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331078.1"
                     /translation="MTVAPKKSRTLRASSLVLAAGLALPLLAQAAEDPAVAQLTQRLM
                     AIQANPDASQLAAFERLQAQQAVADLAKAKRRDLDQARYLAERRVEIAETSVRAALAQ
                     REVDQLERTRSELLIEASRRDAQRARQETERLRVQAQIQAEETERLRQSAEQEALARQ
                     DAETALTSVAGKQTARLSAAQQSAAKLAREEAELVSGSKLPGSKFDSRGEVFTFNGDA
                     FAAGKGSLSGGAKGQAKALAEYLNIGKKGRVRIEAYDAAAGVGDQRAQALRDALVAAG
                     VSASRLQVAGKKAAATKARSAEVIIAP"
     gene            complement(4402..4743)
                     /locus_tag="VIO83_07115"
     CDS             complement(4402..4743)
                     /locus_tag="VIO83_07115"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HEY8331079.1"
                     /translation="MACTFALAGAAQAQDGALELMQARQAVDKATQADADQYAPDLIA
                     VARQGLEQAQRAAGDRRERKNAPALAVRATVDADLARARSEEATATAQLQLRRNEVTQ
                     LQRQLATGENR"
     gene            4898..>5913
                     /locus_tag="VIO83_07120"
     CDS             4898..>5913
                     /locus_tag="VIO83_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY8331080.1"
                     /translation="MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPP
                     GMVKKIAYSLMDEGQVPEFERELELNMAIALAEAGRFRVNVFKQRGEVGMVIRAIRSK
                     IPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
                     PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFA
                     ETGHLCLATLHSNNADQTIERILNFFPESAHRNVLMNLSLNLRAVVSQRLVKGKDGRR
                     LPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCLFRMAKNGIIEQEEA
                     LRAA"
BASE COUNT          952 a         1917 c         2014 g         1030 t
ORIGIN      
        1 agcgcacctt ccagatccac ctgcagatcc cccagctcct ccacgcccac gctcaaccgc
       61 accagcgcat cgctgatgcc cagctgggcg cggcgttcca ccgggatcga ggcatgggtc
      121 atcaccgccg ggtggttgac caggctttcc acgccgccga gcgactcggc caaggtgaac
      181 agctcggtct tctcgcagaa gcgctttgcg gcctcgaaac cgcccttgag gacgatcgag
      241 acaatgccgc cgtagccgtt catctggcgc gtggccagtt catgctgcgg gtgcgaggcc
      301 aggccggggt agatcacttt ctctaccgcc gggtgggtct ccagccactg ggccagggcc
      361 agcgcgttgg cgcaatgggc cttcatgcgc agcggcagcg tcttcaggcc acgcagggcc
      421 aggaagctgt cgaacgggcc ctgcacgccc ccgatggagt tctgcaggaa ggccatctgt
      481 tcggccagct cggcgttgtc gcccaccacc accatgccgc caaccatgtc cgagtgcccg
      541 ttgaggtact tggtggccga gtgcaggacc aggtccgcgc ccagttccag cgggcgctgc
      601 agcatcggcg aggcgaaggt gttgtccacc accacgacca ggccgtggcg cttggcgatg
      661 gcactcaccg cggcgatgtc cacgatcttc agcatcgggt tggtcggggt ttcgatccag
      721 accatcttgg tctgcggggt gatcgaggcc tcgaacgcgg ccaggtcggt caggtcgacg
      781 aagctgaact gcaggttggc ggtgcgcttc cgcacgcgct cgaacaggcg gaaggtgccg
      841 ccgtagatgt catccatcgc cacgacgtgg ctgccggcgt ccagcagctc catcacggtg
      901 gagctggcgg ccatgcccga ggcgaacgcg aagccgcggc tgccgccttc cagggaggcc
      961 acgcaacgct cgtaggcgaa gcgggtcggg ttgtgggtac gcgagtactc gaagccctgg
     1021 tgttcaccgg ggctggactg ggcgtaggtg gaggtggcgt agatcggcgg catcactgcg
     1081 ccggtgcttg gatccggcga ctgtccgccg tggatggcca gggtagccag ggcaagcgca
     1141 cggttgccgt gcccggagga agcgtggtcg gacatgttgt ttcccagcaa ggagagcccc
     1201 ggtgggggct cccgcaaaag gagcggaagt ctatcagcgg cggcttaatc ggccgttgac
     1261 gcggataaac gcagattcag agcagcggcg gccggggcca ggcgcttact gaacgcggcg
     1321 gcgcaggtag ttcagcaggt cgatgcgggt gatcaggccc aggaacgtgc cgccgtccat
     1381 gacgatggcc acctggcccc ggtcgaacac cggcagcagc gcctcgatcg gggacttcac
     1441 atccagccgg tccagcttgc tgaccatggc cgtggccacc gggtcgcgga agcgcgcttc
     1501 gtcgccatac acatgcagca ggacatcgct ttcatccacg atgccgacca gctggtcgcc
     1561 gtccatcacc ggcagctgcg acacgtcgta cagcttcatg cgctggtagg cggtggtcag
     1621 caggtcgttc gggccgatca cgacagtgtc gcgctggccg tagggacgca ggatcaggtc
     1681 gcgcaggtcg ccgctctgcg ggcgctccag gaaaccgttg tccagcatcc agtagtcgtt
     1741 gtacatcttg gacaggtact tgttgccggt gtcgcagacc agcaccagca ccttcttcgg
     1801 ggtggtctgc tccttgcagt acttcagcgc cgccgccagc agggtgccgg tggacgagcc
     1861 gccgaggatg ccttccttgc ccagcagctc gcgcgcggta tggaagctct ccgcgtcggt
     1921 gatggcatag gccttggtga cccggctgaa atcggcaatg gagggcagga agtcttcgcc
     1981 gatgccttcc accagccagc tgcccgactt gtcgttgagc acgccgtcgt tgatgtactc
     2041 ggccaggatc gagccgaccg ggtcggccag gaccagctcg gtcttcggcg aggcgctggc
     2101 gaaggcgcgg gacaggccgg tcatggtgcc cgagctgccg cagccgaaca cgatcgcgtc
     2161 caggtcgccg cccatctgct ccaggatctc cgggccggtg ccgaactcat gggcggccgg
     2221 gttgtccggg ttgccgaact ggttgatgaa gtatgcgccc ggggtttcct cggccacgcg
     2281 cttggccatg tcctggtagt actccgggtg gcccttggcc acgtccgaac gggtcaggcg
     2341 cacttcggcg cccatcgcct tcaggttgaa gatcttctcg cggctcatct tgtcgggcac
     2401 gaccaggatc agcttgtagc ccttctgctg ggcgaccagg gccaggccca ggccggtgtt
     2461 gccggcggtg ccctcgacca gggtcgcgcc cggggtgagc tcgccgcgct tttcagcggc
     2521 ctcgatcatc gacaggccga tgcggtcctt gatcgatcct ccgggattgg cgctctccag
     2581 cttcaggtag agctcgcaga caccggtgtc caggcgctgg gccttgacga tcggggtgtg
     2641 cccaatgagg tcgagtacgg aggagtggat tgccatcgga tcacatcgtt cgcgccgcaa
     2701 tgcggccgga ccggtgatta tccgacggtt gacgcggaaa gtcaggttgt ggcgattaaa
     2761 ggccgatgcc gggctgtagg cggcggggtg atgccggccg cgacagcgga gcgacgcgaa
     2821 cggcgcccgt cgacgaggtg acggacgccg cccgcggcgt tgggtcaaaa ccttacatca
     2881 atgcatgggg gagtcgtaca tccgttccag cttctgcttg gccagcagct tctcacgctt
     2941 catctggccc aaggtgagat cgtctatcgg cagtacaccg agttgggcat ccatgcactt
     3001 tttgttcaac ttctggtgcc gttcgtaaag tgccttgaat tccggatcac gcttgcggcg
     3061 tgcgtcgatc tcgctctggg gctgaccttc aaacatgatg aacctcctat cgctgtacgg
     3121 cagaaacaag aacgccccgg cctgacggca cggggcgttg cttttgggtg gagcacctga
     3181 cgatgaccgc gaccgaaccc gcatgcgcaa ccgggactgc ccggactgca gactgctgtg
     3241 gttcgtgccc tcggttcatc ggcccggccc tccaatcgtc cgggtcgcgg aaggcgcccc
     3301 ggacaactga ccctacgcct gcccgaaggg gtcttcaagc gtttcatctg aaattttcga
     3361 tgcgctccag ccggggttca ggctgaacgc gcatttcaac gaaaattaaa caaaaacagg
     3421 tagttgcgtc atgaccaacg gtcatgaccg acccgctgcc agcccacggc atgagccgcg
     3481 cagaaccggc tcagggggcg atgatgacct cggccgaacg cgccttggtg gccgcggcct
     3541 tcttgcccgc cacctgcagc cggctggcgc tgacgccggc ggccaccagg gcgtcgcgca
     3601 gggcctgggc gcgctggtcg cccaccccgg ccgcggcgtc ataggcctcg atccggaccc
     3661 ggcccttctt gccgatgttc aggtactcgg ccagtgcctt ggcctggccc ttggcgccac
     3721 cggacaggct gcccttgccg gccgcgaagg cgtcgccatt gaaggtgaac acttcgccgc
     3781 ggctgtcgaa cttcgacccc ggcagcttgc tgcccgagac cagttcggct tcctcgcggg
     3841 ccagcttggc cgcgctctgc tgggccgcgc tcaggcgggc ggtctgcttg ccggccacgc
     3901 tggtcagcgc cgtttcggcg tcctggcggg ccagggcctc ctgctcggcc gactgacgca
     3961 ggcgctcggt ttcctcggcc tggatctgcg cctgcacgcg cagccgctcg gtttcctgcc
     4021 gggcgcgctg ggcgtcgcgg cggctggctt cgatcagcag ctcgctgcgg gtacgttcca
     4081 gctggtccac ctcgcgctgg gccagggccg cacgcacgct ggtttcggcg atctcgaccc
     4141 ggcgttcggc caggtaacgg gcctggtcca ggtcgcggcg cttggccttg gccaggtcgg
     4201 ccacggcctg ctgggcctgc aggcgctcga aggcggccag ctgcgaggcg tcgggattgg
     4261 cctggatggc catcaggcgc tgggtcagct gggccacggc cgggtcttcg gcggcctggg
     4321 ccagcagcgg cagggccagg ccggcggcca gcaccaggct ggacgcgcgc agggtgcggg
     4381 acttcttcgg cgcgacggtc atcaacggtt ctccccagtg gcgagctggc gttgcagctg
     4441 ggtcacttca ttgcgacgca gctgcagctg ggcggtggcg gtggcttctt cgctgcgggc
     4501 gcgggccagg tcggcatcca cggtggcgcg cacggccagg gccggggcgt tcttgcgctc
     4561 gcggcggtcg ccggcggcgc gctgggcctg ttccaggcct tggcgggcca cggcgatcag
     4621 atccggggcg tactggtcgg catcggcctg ggtggccttg tccaccgcct gccgggcctg
     4681 catcagttcc agggcgccgt cctgggcctg ggcggcgcct gcgagcgcga atgtgcacgc
     4741 catcacgcaa acaatcatga ccaccgacgg gatacgtcgt atttgtgcga agctattcat
     4801 tgggggtgcc gtcgccagaa gtaggagcgc ggccattgtc ggaagcctgc ggcgtgcttg
     4861 caatggcacg cggccgattg ttggggaaac atttcgcatg gatatcggct atttcctgaa
     4921 gctgatgacc gaaaagaacg cgtcggacat gttcttgacc accggcgccc cggtctacat
     4981 caagatcgag ggcaagctgt acccgttggg gaacaccggc ttgcccccgg gcatggtcaa
     5041 gaaaatcgcc tactcgctga tggacgaggg ccaggtgccc gagttcgagc gcgagctgga
     5101 gctgaacatg gccatcgccc tggccgaggc cgggcgtttc cgcgtcaacg tgttcaagca
     5161 gcgcggcgag gtcggcatgg taatccgtgc gatccgcagc aagatcccca gcatcgaaga
     5221 gctcaacctg ccgcaggtgc tcaaggacgt catcatgacc ccgcgcgggc tggtgctggt
     5281 ggtgggctcg accggttcgg gcaagtccac ctcgctggcg tcgatgatcg accaccgcaa
     5341 cagcaccacc accggtcaca tcctcaccat cgaggatccg atcgagtacc tgcacaagca
     5401 caagatgtcc atcgtcaacc agcgcgaggt ggggctggat acgcacgcct tccacaacgc
     5461 actgaagaac gcgatgcgtg aagcgccgga cgtgatcctg atcggcgaga tcctggatgc
     5521 cgaaaccatg gaggcggcca tcgccttcgc cgagaccggg cacctgtgcc tggccacgct
     5581 gcactccaac aacgccgacc agaccatcga gcgcatcctc aacttcttcc cggaaagcgc
     5641 gcaccgcaac gtgctgatga acctgtcgct gaacctgcgc gcggtggtgt cccagcgcct
     5701 ggtgaagggc aaggacgggc gccgcctgcc ggccaccgag gtgttgatca acaccccgat
     5761 gatccgcgac ctgctgcgcc gcggccaggt gcacgagatc aaggcggcga tggaggcctc
     5821 gctggaagaa ggcatggaga gcttcgacca gtgcctgttc cggatggcca agaacggcat
     5881 catcgagcag gaggaagcgc tgcgcgcggc cga
//
LOCUS       DATCUN010000177         3114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_157646, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000177 DATCUN010000000
VERSION     DATCUN010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157646"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VIO83_07125"
     CDS             <1..208
                     /locus_tag="VIO83_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331081.1"
                     /translation="RFVPDDIHVVAEVPRTLSGKKQELPVKKLLLGQPIEKVVNKDAM
                     ANPQCLDWYVAFAARRAVPAAAVG"
     gene            complement(258..1193)
                     /locus_tag="VIO83_07130"
     CDS             complement(258..1193)
                     /locus_tag="VIO83_07130"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8331082.1"
                     /translation="MNLRQLRQFVTLAETGNFHRAAELLHMAQPPLSVSIRKLEEEMG
                     DALFERRSTGVFITPVGEAMLADARLTLFHAEQCRQAVLDARQGQGGLLRMGIIGSAT
                     YALLPELIPSLRERFPKIELELTEATSSEILDGLVSRRFDVGFVRYPVLHPAPFELLP
                     MDRDDFVLAVSERSPLAAQEAIALSEAAHEPFIMYPRDKVPSLSALALMRCQLSGFTP
                     HVAQEAMQVQTIMSLVASGLGVGLVAGVARLVMPRGVKCLALTDNPPGFHVGIALARL
                     QGNTSRLVERFTEHALRFSTAPDVAPPPAAQPSAD"
     gene            1323..2309
                     /locus_tag="VIO83_07135"
     CDS             1323..2309
                     /locus_tag="VIO83_07135"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8331083.1"
                     /translation="MHKRRTILGALGGAALITLGAATLAPVAQAQTFPSKTIKFVVPF
                     GPGSGTDTSARYFARKLQDITGQSVVVENKPGANGFIAVKQVLAAPADGYTVFIGSNS
                     TLAVNAALFKELPYDPVKDFAPLTMMMRSPAMLTVTPQAPYQDLKGLLAYAKANPGKI
                     NFGAGSAGYQLMGELLNDAAKVQTVHVPFKGAGETMTAVASGTVDYAFAEVTAVQELA
                     KGGRVKVLAVAADKRVASSPDVPTASEAGLPGFEAYTWVGAMVSAKTPAAETARLAEL
                     FTAISRTDETRAFYERIGATPMTGGPAQMHEFQKNEIALWKRIVHQAKVPQQ"
     gene            2306..>3114
                     /locus_tag="VIO83_07140"
     CDS             2306..>3114
                     /locus_tag="VIO83_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY8331084.1"
                     /translation="MSERAAMPPGALQHVKVLDLSRVLAGPWAGQTLGDLGAEVIKVE
                     RPGTGDDTRAWGPPYVTDPAGQSTGESAYYMCTNRNKQSVTVDFTRPEGQQIVRQLAA
                     QCDVLIENFKTGGLTQYGLDYASLRALNPRLVYCSVTGFGQDGPYAHRAGYDFLIQGM
                     GGLMSITGRSDGEPGGGPMKVGVALTDILTGLYASTAILAALQAREHTGRGQHIDLAL
                     LDVGVACLANQGMNYLYGDRVPQRMGNAHPNTVPYQDFPTADGHMILAIGN"
BASE COUNT          551 a         1041 c         1049 g          473 t
ORIGIN      
        1 gcgcttcgtg cccgacgaca tccatgtggt ggccgaggtg ccgcgcacgc tgagcggcaa
       61 gaagcaggag ctgcccgtca aaaaactgct gctgggccag cccatcgaga aggtggtcaa
      121 caaggatgcg atggccaacc cacagtgcct cgattggtac gtggcgttcg ccgcgcgccg
      181 cgcggtgccg gccgctgccg tcggctgaac gccgcgcctg ctgcgggatg gcgcactggc
      241 gttgcggggg caagccttca gtcggccgac ggctgtgccg cgggcggcgg cgccacatcg
      301 ggcgctgtgg aaaagcgcag ggcatgctcg gtgaagcgct cgacgagccg gctggtattg
      361 ccttgcagcc gcgccagcgc aatgcccaca tgaaaccccg gcggattgtc ggtcagtgcg
      421 aggcatttca cgccacgcgg catcaccagc cgcgccacgc cggcgaccag ccccacgccc
      481 aggcccgagg ccaccaggct catgatggtc tgcacctgca tggcctcctg ggccacatgc
      541 ggcgtgaagc ctgagagctg gcagcgcatc agcgccaggg ccgagaggct gggcaccttg
      601 tcccgcgggt acatgatgaa gggctcgtgt gccgcttcgg acagcgcgat ggcctcctgc
      661 gccgcgagcg ggctgcgctc cgagacggcg aggacgaagt catcgcggtc catgggcagt
      721 agctcgaacg gggccgggtg cagcacgggg tagcgcacaa aacccacatc gaagcggcgc
      781 gagaccaggc cgtcgaggat ttcggacgag gtggcctcgg tcagttcgag ctcaatcttg
      841 ggaaaacgct cgcgcagcga ggggatcagc tcgggcagca gcgcatacgt cgccgagccg
      901 atgatgccca tgcgcagcag tccaccctgg ccctggcgcg catccaacac ggcctggcgg
      961 cattgctcgg cgtggaacaa cgtgagccgc gcatcggcca gcatggcctc gcccaccggg
     1021 gtgatgaaga cgcccgtgga gcggcgctcg aacagcgcat cgcccatttc ttcttcgagt
     1081 ttgcggatgg acaccgacaa cggcggttgc gccatgtgca gcagctcggc cgcgcggtgg
     1141 aagttacccg tttccgcgag cgtgacgaat tggcgcagtt ggcgcaggtt catgtgatat
     1201 ccaaatggta ttggcttttc aattcttaat attagacggc actagtcctg ctggctagag
     1261 tgcgagccaa ggccccacgg agggcccggc aaacctgccc ccattccagg agacaaattc
     1321 ccatgcacaa gcgcagaacc attctgggcg ccctcggcgg cgctgccctg atcacgctgg
     1381 gcgccgccac cctcgcgccc gtcgcccagg cgcagacctt cccatcgaag accatcaagt
     1441 tcgtcgttcc cttcgggcct ggcagcggca ccgacacctc tgcgcgctat ttcgcgcgca
     1501 agctccagga catcacgggc cagtccgtgg tggtcgagaa caagccgggc gccaacggct
     1561 tcatcgccgt caagcaggtg ctcgcggcgc cagccgacgg ctacaccgtg ttcatcggca
     1621 gcaattcaac gctggccgtg aacgccgcgc tgttcaagga gctgccctat gacccggtca
     1681 aggatttcgc accgctgacg atgatgatgc gctcgcccgc catgctcacc gtcacgccgc
     1741 aggcgccgta ccaggacctc aaaggcttgc tggcctacgc caaggcaaac cccggcaaga
     1801 tcaatttcgg cgccggctcg gcgggttacc agctcatggg cgagttgctc aacgatgcgg
     1861 ccaaggtgca gaccgtgcat gtgccgttca agggtgcagg cgagaccatg acggccgtgg
     1921 cctccggcac ggtggactat gcctttgccg aggtgaccgc cgtgcaggag ctggccaagg
     1981 gtggccgcgt gaaggtgctc gccgtggcgg ccgacaagcg cgtggcctcg tcgcccgacg
     2041 tgcccaccgc cagcgaggcc ggcttgcccg ggttcgaggc ctacacctgg gtgggcgcca
     2101 tggtcagcgc caagacgcca gcggccgaga cggccaggct cgccgagctg ttcacggcga
     2161 tctccaggac ggacgagacc cgtgccttct acgagcgcat tggcgccaca cccatgaccg
     2221 ggggccccgc gcagatgcac gagttccaga agaacgagat cgcgctgtgg aagcgcatcg
     2281 tgcaccaggc caaggtgccg cagcaatgag cgaaagggcc gccatgccgc caggagcgct
     2341 ccagcatgta aaggtgctgg atctttcgcg cgtgctcgca gggccgtggg ccggccagac
     2401 actgggggac ctgggggccg aagtgatcaa ggtcgagcgc cccggcaccg gcgacgacac
     2461 ccgcgcctgg ggcccgccgt atgtgacgga cccggcgggc cagtccacgg gtgagtcggc
     2521 ctactacatg tgcaccaacc gcaacaagca gtcggtgacg gtggatttca cgcggcctga
     2581 gggccagcag atcgtgcgcc agctcgcggc gcagtgtgac gtgctcattg agaatttcaa
     2641 gaccggcggc ctcacgcagt acggcttgga ttacgcgagc ctgcgcgcgc tcaacccgcg
     2701 cctggtgtat tgctcggtca ccggcttcgg ccaggacggg ccctatgcgc accgcgcggg
     2761 gtatgacttt ctgatccagg gcatgggcgg gctcatgagc atcactggcc gctccgatgg
     2821 cgagcccggc ggcggcccca tgaaggtggg ggtggccctc accgacatcc tcaccggcct
     2881 gtatgccagc acggccattc tggccgccct gcaggcgcgc gagcacacgg gccggggcca
     2941 gcacattgat ctggcgctgc tcgacgtggg cgtggcctgc ctggccaacc agggcatgaa
     3001 ttacctgtat ggcgacaggg tgccgcagcg catgggcaac gcgcacccca acaccgtgcc
     3061 ctaccaggac tttccgaccg ccgacggcca catgatcctg gccattggca acga
//
LOCUS       DATCUN010000178         5241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_101529, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000178 DATCUN010000000
VERSION     DATCUN010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101529"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..665)
                     /locus_tag="VIO83_07145"
     CDS             complement(138..665)
                     /locus_tag="VIO83_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEY8331085.1"
                     /translation="MKGARLIIGSMLLLGLTACDGSGDFSDLQAYMNEVRAKPKGVVE
                     PLPQFQPYEAFTYSAAAMRHPFQPPVRVDLQKRQKGSAEIKPDESRPKQFLEGFNIEM
                     FQMVGLLANDGGRFALVSGVGGVHRVKVGDYLGRNYGRIVAINDDRIEVVEIVTDGEG
                     GWLERPRTLTLKERS"
     gene            complement(662..1285)
                     /gene="pilO"
                     /locus_tag="VIO83_07150"
     CDS             complement(662..1285)
                     /gene="pilO"
                     /locus_tag="VIO83_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEY8331086.1"
                     /translation="MSLAQSLESLRKVNLSELDANNLGSWPAPVKVIAGALLLIGVLA
                     LGYNFHLKDLQAELEQRGAEEVTLKQQFETKAFQAANLEAYRQQMVEMEQSFGALLRQ
                     LPGDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQIRVVGAYHDLATF
                     VSGAASLPRIVTLHDFEIKPVAKDGEAKLSMSILAKTYRYNDKGLQK"
     gene            complement(1282..1908)
                     /locus_tag="VIO83_07155"
     CDS             complement(1282..1908)
                     /locus_tag="VIO83_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEY8331087.1"
                     /translation="MARINLLPWREHRREERKQRFLVALGAVLVGAAALVFAGDQYLN
                     GAIEQQNARNDYVRKEIAALDARIKEISELKKRREELLERMKIIQDLQGNRPVTARVF
                     DQLVRTLPDGVHFTALKMTGKNIAIQGAAESNNRVSALMRNLDASDWLAAPNLTEVKA
                     VTAGTVDQANVFQLTVQQTRPDEEGAAQASASMAAGKAQQANSKGATK"
     gene            complement(1908..2972)
                     /locus_tag="VIO83_07160"
     CDS             complement(1908..2972)
                     /locus_tag="VIO83_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY8331088.1"
                     /translation="MLGLFNKTANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPL
                     PLNAVVEKNIAEIEGVGQALSRVLAKARTGARNVAVAVAGSAVITKTIEMDAGLSEDE
                     LENQLKVEADQYIPYPLEEVAIDFEVQGVSPRNPERAEVLLAACRKENVEVREAALAL
                     AGLTAKVVDVEAYALERAFALLEPRLVDGRHELTVAVVDVGATMTTLSVLHNGKTIYT
                     REQLFGGKQLTEEIQRRYGLSFDEAGLAKKQGGLPDDYDSEVLLPFKEAVVQQVSRSL
                     QFFFAAGQFSDVDCILLAGGTASLPDLDRMIQQKIGTPCVVANPFADMALNSKVNAAA
                     LASDAPSLMIACGLAMRSFD"
     gene            3157..>5241
                     /locus_tag="VIO83_07165"
     CDS             3157..>5241
                     /locus_tag="VIO83_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEY8331089.1"
                     /translation="MRLLKLLSWTCIALISAVMLGFSGAFLYLSPGLPSVETLRNVRM
                     QVPLRIYSADDKLIAEFGEMRRSPIQFANIPPDFINALLAAEDDNFANHYGVDVKSLM
                     RAAAQLLQSGQIQTGGSTITMQVAKNFFLSNERSFARKINEILLALQIERALDKNAIL
                     ELYVNKIYLGNRAYGIEAAAQIYYGKSIGELSLAQLAMIAGLPKAPSRYNPVANPERA
                     KIRRDWILARMYELGKIDQQRYQTALAETVEAHQQAVRPPELEAPYVAEMARAEIVGR
                     FGGDAYTEGYRVHTTVRSDLQTEANQALREGLLNYDQRHGYRGPEARFPALDPAGWLA
                     KLAEQRTLGGLEPAIVTTIGSDGIQVLTRDGSEQPIAWQSMKWARPHINSNSLGRQPG
                     KPAEVVQKGDLIRVLRQEDGSLRFVQLPQAQGALVSLDPADGAIRALTGGFSFEQSNY
                     NRATQAKRQPGSSFKPFVYSAALDKGFTAASLINDAPIEFVDPYSGEVWRPKNDNNTF
                     LGPIRMREALYKSRNLVSIRLLQEIGIEYALDYSKRFGLPREELPRNFSLALGTANLT
                     PLEVTTGYAVFANGGYHVQPYLITQIDNRDGEPLFRANPPRIPDHLTGAGASQATLPV
                     AVTSAIPEGAPPAETPPTAERVLDPRTAYIMTSMLQDVIKRGTGRRALSLQRSDLAGK
                     TGTTNEQKDSWFA"
BASE COUNT          983 a         1820 c         1515 g          923 t
ORIGIN      
        1 cctgcagatt ggcagccagg actgccggcg aaagcattgc agccagcaag gtcaggctga
       61 agcgcgaaat ggaactgttc attgtctgaa tccgttgtgc agaccgagtt ggattgttca
      121 tgcgtggacc cccgccctca ggaacgttct ttgagggtca gagtgcgcgg ccgctcgagc
      181 cagcccccct cgccgtcagt aacaatttcg acgacctcga tgcggtcatc attgatcgcc
      241 acgatgcgcc cgtagttgcg cccgaggtag tcgccaacct tgacccgatg caccccgcca
      301 acaccactga ccaaggcgaa gcgaccacca tcgttggcca gcaggccgac catctggaac
      361 atctcgatat tgaaaccttc aaggaactgc ttgggtctcg actcgtccgg cttgatctca
      421 gccgagccct tctggcgctt ctgcagatcg acacgcaccg gcggctggaa cgggtggcgc
      481 atggccgcgg cactgtaggt gaaggcctca tagggctgga actgcggcag cggctcgaca
      541 acccccttgg gcttggcgcg cacctcgttc atgtaggcct gcaggtcact gaaatcaccg
      601 ctaccgtcgc aggcggtcag gccgagcagc agcatgctgc cgataatcag gcgggctccc
      661 ttcatttctg cagccccttg tcgttgtagc gataggtctt ggccaggatg ctcatgctca
      721 gtttggcctc gccgtccttg gccaccggct tgatctcgaa atcatgcagg gtgacgatcc
      781 gcggcaggct ggcagcgccg ctgacgaagg tagccaggtc gtgataggcg ccgaccacgc
      841 ggatctggat cggcagttcg atgtagaact gctgggtaac ttccggcagc agcttgatct
      901 cctcgaactc caggccgctg cccagaccgg tacgggtaat gtcctccagc aggccaggca
      961 cttcggtatc gcccggcagc tggcgcagca gcgcgccgaa ggactgctcc atctccacca
     1021 tctgctgccg gtaggcctcc agattggcgg cctggaaggc cttggtctcg aactgctgct
     1081 tgagggtgac ctcttcggcg ccccgctgct ccagctcggc ctgcagatcc ttgaggtgaa
     1141 agttgtaacc cagcgccagc acgccgatca gcagcagggc cccggcgatc accttcaccg
     1201 gtgccggcca ggaaccgaga ttgttggcgt caagctcact gagattgacc ttgcgcaggc
     1261 tttccaggga ttgagccaga ctcatttcgt ggctcctttg ctattggctt gttgggcttt
     1321 tccagccgcc atgctggcgc tcgcctgcgc agcgccctcc tcatccggcc gggtttgctg
     1381 gacggtcagc tggaatacgt tggcctgatc cacggtaccg gcagtcactg ccttgacctc
     1441 ggtcaggttg ggcgccgcca gccagtccga ggcatcgagg ttgcgcatca gcgcggacac
     1501 ccggttgttc gactccgccg caccctggat cgcgatgttc ttgccggtca tcttcagcgc
     1561 ggtgaaatgc acgccatccg gcagggtacg caccaactgg tcgaacaccc gcgcggtgac
     1621 cggacggttg ccctgcagat cttggatgat cttcatccgc tcgaggagct cctcgcggcg
     1681 tttcttcagc tcgctgatct ccttgatccg tgcatccagc gcagcgattt ccttgcgcac
     1741 atagtcattg cgtgcgttct gctgctcgat tgcaccattc aggtactgat caccggcgaa
     1801 caccaaggct gcggcgccga ccagcaccgc ccccagcgcc accaggaaac gctgcttgcg
     1861 ctcctcgcgg cgatgctcgc gccagggtag aaggttgatt ctggccatca gtcaaaactc
     1921 ctcatcgcca gcccgcaggc gatcatcagc gatggggcat cgctggccag cgcggcggcg
     1981 ttcaccttgc tgttcaatgc catgtcggcg aacggattgg ccaccacgca gggggtgcca
     2041 atcttctgct ggatcatccg atcgagatcc ggcagcgatg ccgtaccacc ggccagcagg
     2101 atgcagtcga catcgctgaa ctgaccggcc gcgaagaaga actgcagcga gcgcgacacc
     2161 tgctgcacca ccgcctcctt gaacggcaac agcacttcgc tgtcgtagtc atcgggaagg
     2221 ccgccctgtt ttttcgccag gccggcttca tcgaaggaca ggccatagcg ccgctggatc
     2281 tcctcggtca gctgcttgcc gccgaacagc tgctcacggg tatagatggt cttgccgttg
     2341 tgcaggacgc tgagggtggt catggtcgca ccgacgtcca ccactgcgac ggtcaactcg
     2401 tgacgcccgt cgaccagccg cggctcgagt agggcaaagg cccgctccag cgcataggcc
     2461 tcgacgtcga ccaccttggc ggtcagaccg gccagcgcca gcgcggcttc gcgcacctcg
     2521 acgttttctt tgcggcaggc ggccagcagc acctcggcgc gttcggggtt gcgcggcgag
     2581 acaccctgca cctcgaagtc gatggccacc tcttccagcg gatagggaat gtactggtcc
     2641 gcctcgacct tcagctggtt ttccagctcg tcctcagaca atccggcatc catctcgatg
     2701 gtcttggtga tcaccgccga accggccaca gccacggcca cgttccgggc gccagtacgt
     2761 gccttggcca gcacccggct gagcgcctgc cccacgccct cgatctcggc gatgttcttc
     2821 tcgacaaccg cattcagcgg caagggctcg accgcatagg cctcaacctt gtagcgcccc
     2881 cccgagcggc tcagctccag cagcttgact gaagtcgaac tgatgtcgat ccccaatagc
     2941 gtattcgcgg ttttattgaa gagccctagc acggccaatt tcctatcagc atccgaaagt
     3001 tacggacggt tcatgtattt cagcattaaa tgcccccact tgacagctgg gcaaatggct
     3061 acttcaggga aaaaacacct ataatgtgaa cagcttttgc cttttccctg cgccacccac
     3121 ccagcccatt cctctccctg gacaagcaag atcccgatgc gtctgttgaa gcttctgtcg
     3181 tggacctgta tcgccctgat cagcgcagta atgctgggat tcagtggcgc ctttctctac
     3241 ctcagccccg gcctgccgtc ggtggaaacc ctgcgcaacg tgcgcatgca ggtcccgctg
     3301 cgcatctaca gcgccgacga caagctgatc gccgagttcg gcgaaatgcg ccgcagcccc
     3361 atccagttcg ccaacatccc cccggacttc atcaacgcct tgctggccgc ggaggacgat
     3421 aatttcgcga atcattatgg cgttgacgtc aaaagtctga tgcgggctgc cgcgcaatta
     3481 ttgcagagcg ggcagatcca gaccggcggc agcaccatca ccatgcaggt ggcgaagaac
     3541 ttcttcctct ccaacgagcg cagctttgcc cgcaagatca acgagatcct gctcgccctg
     3601 cagatcgagc gggcgctcga caagaacgcc atcctcgagc tctacgtcaa caagatttac
     3661 ctcggcaacc gcgcctacgg catcgaggcg gcggcgcaga tctactacgg caaatcgatc
     3721 ggcgagctgt cgctggccca gctggcaatg atcgccggcc tacccaaggc cccctcgcgc
     3781 tacaacccgg tagccaaccc ggagcgcgcc aagatccgcc gcgactggat cctcgcgcgc
     3841 atgtacgagc tgggcaagat cgaccagcag cgctaccaga ctgcgctggc cgaaaccgtc
     3901 gaagcccacc agcaagccgt cagaccgccg gagctggaag cgccctatgt ggcggaaatg
     3961 gcccgcgccg aaatcgtcgg ccgcttcggt ggcgacgcct ataccgaagg ctaccgcgtg
     4021 cacaccaccg tgcgcagcga cctgcagacg gaagccaacc aggccctgcg cgaaggcctg
     4081 ctcaactacg accagcgcca cggctatcgt ggacccgagg cgcgcttccc cgccctcgac
     4141 ccggccggct ggctggccaa actggccgag cagcgcacgc tgggcggact cgagcccgcc
     4201 atcgtcacga ccattggcag tgacggcatc caggtgctga cccgcgacgg cagcgaacag
     4261 ccgatcgcct ggcagagcat gaaatgggcc cgcccccaca tcaacagcaa cagcctcggc
     4321 cgccagcccg gcaagccggc cgaggtggtg cagaagggcg acctgatccg cgtcctgcgc
     4381 caggaggacg gcagcctgcg cttcgtccag ctgccgcagg cgcaaggcgc cctggtctcg
     4441 ctggacccgg ccgacggcgc gatccgcgcc ctgaccggcg gcttctcctt cgagcagagc
     4501 aactacaatc gcgccaccca ggccaagcgc cagcctggct ccagcttcaa gcccttcgtc
     4561 tacagcgctg cgctggacaa gggctttacc gccgccagcc tgatcaacga cgcacccatc
     4621 gagttcgtcg acccctacag cggcgaggtc tggcgcccga agaacgacaa caacaccttc
     4681 ctcggcccga tccgcatgcg cgaggcgctg tacaagtcgc gcaacctggt atcgatccgc
     4741 ctgctgcagg aaatcggcat cgaatacgcc ctcgactaca gcaagcgctt cggcctgccg
     4801 cgcgaggagc tgccacgcaa cttctcgctg gcgctcggca ccgccaacct gaccccgctg
     4861 gaagtcacca ccggctatgc ggtattcgcc aacggcggtt atcacgtcca gccttacctg
     4921 atcacgcaaa tcgacaaccg cgatggcgag ccgctcttcc gcgccaaccc gccgcgcata
     4981 ccggaccacc tgaccggcgc tggcgcatcg caagcgaccc tgccggtcgc cgtgacgagc
     5041 gcaatccccg agggcgcccc gcccgccgaa acgccaccga ccgccgagcg cgtcctcgat
     5101 ccgcgcaccg cctacatcat gaccagcatg ttgcaggacg tgatcaagcg cggcaccggc
     5161 cggcgcgccc tgagcctgca gcgcagcgac ctggccggca agacgggcac caccaacgaa
     5221 cagaaggact cctggttcgc c
//
LOCUS       DATCUN010000179         2730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_214126, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000179 DATCUN010000000
VERSION     DATCUN010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214126"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VIO83_07170"
     CDS             <1..227
                     /locus_tag="VIO83_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY8331090.1"
                     /translation="GKVERIVDFGAFVNILPGKDGLVHISQISDKRIEKVTDVLKEGE
                     EVKVLVLDVDNRGRIKLSIKDVAAAEASGV"
     gene            complement(303..599)
                     /locus_tag="VIO83_07175"
     CDS             complement(303..599)
                     /locus_tag="VIO83_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014340249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HEY8331091.1"
                     /translation="MKAFLCLLLVALSAPLSAATLRCDRDLVSSGDHTSEVLDKCGQP
                     NSRETIGYRRRGDDWGNYEEVRVEEWVYGPRNGMYYFLRFEGNRLIEIDSDRGR"
     gene            complement(680..>2730)
                     /locus_tag="VIO83_07180"
     CDS             complement(680..>2730)
                     /locus_tag="VIO83_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEY8331092.1"
                     /translation="ASDTPEDPQVSPLADATRAPEAANAAAPKQNAAPATESTTRSTA
                     SAPAQQGADTAAPTLATSESAPQTAAASSTGAWQVLLQDAQLRGWRAHLEDRVPQPAV
                     ALDVGPLDLDLQGFSSSGEQPFSLKLASGVGARGQLEADGQLRLKPLSSQLKVRTRDL
                     DLRLAQAYLSPFIHLELRSGLLASDVAVDLQGSAPLALKVSGRAEVSQLHTLDTLKGR
                     DFVKWQLLNVDGLDYRHGEQLRIDRLRLQQPYARFIINEDLSTNVKDLLIAQPPKPQP
                     APPGKPLHVVVGGVDIADGSANFADLSLTPNFATAIQQLNGRIGTLDNQSSKPATVDI
                     RGKVDRYAPVTIQGQLTPFSPLEQLDITTRFKRVELTTLTPYAGKFAGYKIRKGRLNL
                     DLHYRIEKGRLNADNKLLLEDLQLGERVDSPSATDLPVRLAVALLKDTHGNIDIALPV
                     QGDLNNPEFSVAPIVWQTLRNLILRAVQAPFKLIAGLVDGGDEQLDSVPFAAGSSELD
                     PPARATLDTLAKALQQRPTLRLEVEGMSRQAADGPLLAEQRLQREYQDTWYRMLQRRG
                     DKVPAEASQLKVPEDMQGSLLEGIYRARLKQQPPAEWRELKKPERAARLRQALLDSWA
                     QSSLLLRQLAQDRARAIKAYLVEQGQLADERIYLLEVGSAEAAGDNPRVAVPLHLDSE
                     "
BASE COUNT          362 a          850 c         1023 g          495 t
ORIGIN      
        1 tcggcaaggt cgagcgcatc gtcgacttcg gtgccttcgt caacatcctg ccgggcaagg
       61 acggtctggt gcacatttcg cagatcagcg acaagcgcat cgagaaggtc accgacgtgc
      121 tcaaggaagg cgaagaggtc aaggtgctgg tgctggacgt ggacaaccgc ggccgcatca
      181 agctgtcgat caaggacgtg gcggctgccg aagcctccgg cgtctgatcg acgtgatcgc
      241 gtgacagaaa aggccctccg gggccttttt tgttgtccgc gattcggctg cccggggtga
      301 cgctaacgcc cgcggtcgct gtcgatctcg atcaggcgat tgccttcgaa gcgcaggaag
      361 tagtacatgc cgttgcgcgg gccgtagacc cactcctcga cgcgcacctc ctcgtagttg
      421 ccccagtcgt cgccgcgccg gcggtagccg atggtctcgc ggctgttcgg ctggccgcac
      481 ttgtcgagta cctcgctggt atggtcgccg ctgctgacca ggtcgcggtc gcagcgcagg
      541 gtggcggcgc tgagcggggc actcagggcc accagcagca ggcagaggaa agctttcacg
      601 acagaaccct cacgacggga aagaggccgg cgccgtgggc accggcaagg gtattgatcg
      661 gaccctcgtg gtggtcagtt tactcgctgt ccagatgcag cggcacggcg acgcgcgggt
      721 tgtcaccggc ggcctcggcg ctgccgacct ccagcaggta gatgcgctcg tcggccagct
      781 gcccctgctc gaccagatag gccttgatcg cccgcgcgcg atcctgggcc agctggcgca
      841 gcagcagcga actctgcgcc caggagtcga gcagcgcctg gcgcaggcgc gcggcgcgtt
      901 cgggcttctt cagctcgcgc cattcggccg gcggctgttg cttgaggcgg gcgcggtaga
      961 tgccttcgag caggctcccc tgcatgtcct ccggcacctt gagctggctg gcctcggccg
     1021 gcaccttgtc gccgcggcgt tgcagcatgc ggtaccaggt gtcctggtac tcgcgctgca
     1081 ggcgctgttc ggccagcagc gggccgtcgg cggcctgccg gctcatgcct tcgacttcca
     1141 gacgcagcgt cgggcgctgc tgcagggcct tggccagcgt gtccagggtg gcgcgggccg
     1201 gcgggtccag ttcgctggag ccggcggcga agggcacgct gtccagttgt tcgtcgccgc
     1261 cgtcgaccag tccggcgatc agcttgaacg gcgcctgcac tgcgcgcagg atcaggttgc
     1321 gcagggtctg ccagacgatc ggcgccacgc tgaactcggg gttgttgagg tcgccctgca
     1381 ccggcagggc gatgtcgatg ttgccgtggg tatccttgag caacgccacc gccaggcgca
     1441 ccggcaggtc ggtggcgctg ggactgtcga cccgctcgcc gagctgcagg tcctccagca
     1501 gcagcttgtt gtcggcgttc agccggccct tctcgatgcg gtagtgtagg tcgaggttga
     1561 ggcggccctt gcggatcttg tagccggcga acttgccggc gtagggggtc agggtggtca
     1621 gctcgacccg tttgaagcgg gtggtgatgt ccagctgctc cagtggcgag aagggggtca
     1681 gctggccctg gatggtcacc ggggcgtagc ggtcgacctt gccgcggata tccaccgtgg
     1741 ccggcttgct ggactggttg tcgagggtgc cgatccggcc attgagctgc tggatggcgg
     1801 tggcgaagtt gggtgtcagc gagaggtcgg cgaagttggc cgagccgtcc gcgatgtcca
     1861 cgccaccgac caccacgtgc agcggcttgc cgggcggcgc cggctggggc ttgggcggct
     1921 gggctatcag cagatccttg acgttggtgc tgaggtcctc gttgatgatg aagcgcgcgt
     1981 acggctgttg caggcgcagc cggtcgatgc gcagctgctc gccgtggcgg tagtcgaggc
     2041 cgtcgacgtt gagcagctgc catttgacga aatcgcggcc cttgagggtg tcgagggtgt
     2101 gcagctggct cacctcggcg cggccgctga ccttgagtgc tagcggcgcg ctgccctgca
     2161 ggtcgacggc gacatcgctg gcgagcaggc cgctgcgcag ttccaggtgg atgaacgggc
     2221 tgagataggc ctgggccagg cgcaggtcga ggtcgcgcgt gcgcaccttg agctggctgc
     2281 tcagtggttt caggcgcagc tggccgtcgg cctcgagctg gccgcgggca ccgacgccgc
     2341 tggccagctt gaggctgaag ggttgttcgc cgctgctgct gaagccctgc aggtcgagat
     2401 ccagcgggcc gacgtccagc gctaccgccg gctgcggcac gcggtcctcg agatgggcgc
     2461 gccagccgcg cagctgggcg tcctgcagca gtacctgcca cgctccggtg ctgctggcgg
     2521 ctgccgtctg cggcgcagat tcagacgtgg cgagggttgg cgctgcggtg tcggcaccct
     2581 gctgggctgg ggcgctggcg gtgctccgtg tggtgctctc ggtggcgggc gcggcgtttt
     2641 gcttcggagc ggctgcgttt gcggcttccg gagcccgggt cgcgtcggcg agcgggctga
     2701 cctggggatc ttcgggagtg tccgacgccc
//
LOCUS       DATCUN010000180         3534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_214161, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000180 DATCUN010000000
VERSION     DATCUN010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214161"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VIO83_07185"
     CDS             <1..1111
                     /locus_tag="VIO83_07185"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY8331093.1"
                     /translation="FAVEVCNAVIGVFQPTPQQKLILNLPATIEAATPNVYADQIEWF
                     GRHIDRRDSVLISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVA
                     LNMYTQGVDPQLDFSDIDGVRKVVEECNQLPVHPRHPYVGDLVHTAFSGSHQDAIRKG
                     FAQQDPEGIWEVPYLPIDPADIGRSYEAVIRVNSQSGKGGITYLLEQEYGISLPRRMQ
                     IEFSQVVQRETDRHGLEMSARQIYDLLVEEYLQGPATYSLKGHRLQEENGVTTVEVEV
                     EQGGAVQSLRGTGNGPLEALVAALPVAVEVMDYSEHAIGAGSNAKAAAYLELRIDGGR
                     PLHGVGIDANLTTASFRALFSALNRALRQSEAQAA"
     gene            1314..2030
                     /locus_tag="VIO83_07190"
     CDS             1314..2030
                     /locus_tag="VIO83_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease I family protein"
                     /protein_id="HEY8331094.1"
                     /translation="MRALLALLLVVIPCLSVHAEAPRTFREAKKVAWKLYADNPVDFY
                     CGCAYKGNRVDLDSCGYQPRKNANRAGRIEWEHVVPAWVIGHQRQCWQQGGRKNCTSN
                     DPLFSKAEADLHNLVPVVGEVNGDRSNFGFGMLSAKPSQYGACPFVVDFKQRTAMPPE
                     SSRGTIARIYLYMSERYQLRLSKQDQRLYDIWNRQYPVSAWERQRNQRIACVQGNANP
                     HVGAVDLKRCAPRAKQLAYN"
     gene            2195..2824
                     /locus_tag="VIO83_07195"
     CDS             2195..2824
                     /locus_tag="VIO83_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEY8331095.1"
                     /translation="MQHGSLHVALGLTVLAALIVSGISPYDRATWWLEVSPVLIAAPL
                     LLATGRRFPLTTLLYVLITLHCLVLILGGAYTYARVPAGFWVQELFELGRNPYDKLGH
                     FMQGFVPALIAREILLRGRFVAPGRMLAFLSLCVAMTISAVYELIEWWVALLAGGGAV
                     EFLGTQGDPWDTQADMFWALIGASTGLLGLAWLQDRQIAKLERTNRRVG"
     gene            2993..>3534
                     /locus_tag="VIO83_07200"
     CDS             2993..>3534
                     /locus_tag="VIO83_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8331096.1"
                     /translation="MAAPTLLSLPLLALALGGFVVGSSEYIIMGLLPEVARDLGVSLA
                     DAGLLVSAYAMGVVIGAPLLGPLLGRLPRKQALMWLMGAYSLGNLGCALAPDYAWLVA
                     ARLFTAISHAGFFGCATLLAAELAPPQRRASAMALVLSGLTIANVLGVPLGAWFGQAS
                     SWRMTFFAVAGFGASTLLAQV"
BASE COUNT          569 a         1298 c         1132 g          535 t
ORIGIN      
        1 cttcgccgtc gaggtgtgca acgcggtgat cggcgtgttc cagccgaccc cgcagcagaa
       61 gctgatcctc aacctgccgg cgaccatcga ggcggccacg ccgaacgtct acgccgacca
      121 gatcgagtgg ttcggccgcc acatcgaccg tcgcgacagc gtgctgatca gcctgcacac
      181 ccacaacgac cgcggcaccg gcgtggccgc caccgagctg ggcctgatgg ccggcgccga
      241 ccgcgtggaa ggctgcctgt tcggcaacgg cgagcgcacc ggcaacgtcg acctggtcac
      301 cgtggcgctg aacatgtaca cccagggcgt cgacccgcag ctggacttct ccgacatcga
      361 cggcgtgcgc aaggtggtcg aggagtgcaa ccagctgccg gtgcacccgc gtcaccccta
      421 cgtcggcgac ctggtgcaca ccgccttctc cggctcgcac caggacgcga tccgcaaggg
      481 cttcgcccag caggacccgg aagggatctg ggaagtaccc tacctgccga tcgacccggc
      541 cgacatcggc cgcagctacg aggcggtgat ccgcgtcaac agccagtccg gcaagggcgg
      601 catcacctac ctgctcgagc aggagtacgg catcagcctg ccgcggcgca tgcagatcga
      661 gttcagccag gtggtgcagc gcgagaccga ccgccacggc ctggagatga gcgcgcggca
      721 gatctacgac ctgctggtcg aggaatacct gcagggtccg gcgacctaca gcctcaaggg
      781 ccatcgcctg caggaagaaa acggcgtcac cacggtggaa gtggaagtcg agcaaggcgg
      841 cgccgtgcag agcctgcgcg gcaccggcaa cggcccgctg gaggcgctgg tcgccgccct
      901 gccggtcgcc gtcgaggtga tggactacag cgagcacgcc atcggcgccg gcagcaacgc
      961 caaggccgca gcctacctcg aactgcgcat cgacggcggc cgcccgctgc acggcgtcgg
     1021 catcgacgcc aacctgacca ccgccagctt ccgcgccctg ttcagcgcgc tgaaccgcgc
     1081 cctgcgccag agcgaggcgc aggcggccta acatgcgcca gcgcggcaac accgacgccc
     1141 cggaccgcaa ggttcggggc gctgttcatt cggcccgcga gcaactgcca agctgcgtct
     1201 ggtcggggca caccaccatc gcaagcaatt gttttttctg aattttcgac agctcgcggt
     1261 agaatcgccg gctaccgcat ggatgcgcct ctcagtctgc tgtcgagatc tccatgcgcg
     1321 ccctgcttgc cttgctcctc gtcgtcatcc cctgcctttc cgtccatgcc gaagcccccc
     1381 gcaccttccg cgaagccaag aaggtcgcct ggaagctgta tgccgacaac ccggtggatt
     1441 tctattgcgg ctgcgcctac aagggcaatc gcgtcgatct ggacagctgc ggctaccagc
     1501 cgcgcaagaa cgccaaccgt gccggccgca tcgagtggga acacgtggtg ccggcctggg
     1561 tgatcggcca tcagcgccag tgctggcaac agggcgggcg caagaactgc accagcaacg
     1621 acccgctgtt cagcaaggcc gaggccgacc tgcacaacct ggtgccggtg gtcggcgagg
     1681 tcaacggcga ccgcagcaac ttcggcttcg gcatgctcag cgccaagccc agccagtacg
     1741 gcgcctgccc cttcgtggtc gacttcaagc agcgcactgc catgccgccg gagtccagcc
     1801 gcggcaccat cgcgcgcatc tacctgtaca tgagcgagcg ctaccagctg cgcctgtcca
     1861 agcaggacca gcgcctgtac gacatctgga accgccagta cccggtcagc gcctgggagc
     1921 gccagcgcaa ccagcgcatc gcctgcgtgc agggcaacgc caacccgcat gtcggcgcgg
     1981 tagacctcaa gcgctgcgca cctcgcgcga agcagctggc ctacaactga gggcaccgcg
     2041 cggtgcgccg cgcgcaccac tccgcaacgg aggcgtcgaa tggtgcgcag ggcgcaccct
     2101 acacagctcg ctggcccata tcacgcggcc ggacaatgga cggcacctga tactgcggga
     2161 taaggtgacg gcgagctgac tgaaggacaa ggcgatgcaa cacggatccc tgcatgtggc
     2221 cctaggcctg acggtactgg ccgcgctgat cgtctccggc atctcgccct acgaccgtgc
     2281 cacctggtgg ctggaagtct cgccggttct gatcgccgcg ccgctgctgc tggccaccgg
     2341 gcggcgcttc ccgctgacca ccctgctcta cgtgctgatc accctgcact gcctggtgct
     2401 gatcctcggc ggcgcctaca cctacgcgcg ggtgccggcc ggcttctggg tccaggaact
     2461 gttcgaattg ggccgcaatc cctacgacaa gctcggccac ttcatgcagg gcttcgtgcc
     2521 ggcgctgatc gcccgcgaaa tcctcctgcg cgggcgcttc gtcgcccccg ggcgcatgct
     2581 ggccttcctg tcgctgtgcg tggcgatgac catcagcgcc gtctacgaac tgatcgaatg
     2641 gtgggtggcg ctgctcgccg gcggcggcgc ggtggagttc ctcggcaccc agggcgaccc
     2701 ctgggacacc caggccgaca tgttctgggc gctgatcggc gccagcacgg ggctgctcgg
     2761 cctggcgtgg ctgcaggacc ggcagatcgc caagctggag cgcacaaacc gtagggtggg
     2821 ttaggccgca ggccgtaacc caccgaccgg ctgccaagcc ggcaaaggga tacctggcgg
     2881 catccatggt gggttacgcc gcaagcggct aacccaccct acgcccctcg ccgcgctaga
     2941 atgcgccccc cgcactcctg ccgcccgcca ctcctccgga cttcccttcg ccatggccgc
     3001 cccaaccctg ctttccctgc ccctgctggc cctcgccctc ggcggcttcg tggtcggcag
     3061 cagcgaatac atcatcatgg gcctgctgcc cgaggtggcc cgcgacctgg gcgtcagcct
     3121 ggccgatgcc ggcctgctgg tcagcgccta cgccatgggc gtggtgatcg gtgcgccgct
     3181 gctcggcccg ctgctcggcc gcctgccgcg caagcaggcg ctgatgtggc tgatgggcgc
     3241 ctacagcctc ggcaacctgg gctgcgcgct ggcgccggac tatgcctggc tggtcgccgc
     3301 gcgactgttc actgccatca gccatgccgg cttcttcggc tgcgccaccc tgctcgccgc
     3361 cgagctggcc ccgccgcagc ggcgcgcctc ggccatggcc ctggtgctca gcggcctgac
     3421 catcgccaac gtgctcggcg tgccgctggg cgcctggttc ggccaggcca gcagctggcg
     3481 gatgaccttc ttcgccgtcg ccggcttcgg cgccagcacc ctgctcgccc aagt
//
LOCUS       DATCUN010000181        10600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_26353, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000181 DATCUN010000000
VERSION     DATCUN010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10600
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26353"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1099)
                     /gene="zipA"
                     /locus_tag="VIO83_07205"
     CDS             complement(212..1099)
                     /gene="zipA"
                     /locus_tag="VIO83_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEY8331097.1"
                     /translation="MDIGLREWLIVIGIIVIGGILIDGWRRMRGGRGRLKFKLDRSAA
                     NLPDDDGDKELLGPVRVVDRHQEPSLDEDDLPEAALEPRSKRRDEPLAGDLELGMTEP
                     VAAPLRMPAADDMPAEPRSKDKLREKATEKATEKADKVAAASAPPRDLPPVEEVLVIS
                     VVARDPDGFKGPALLQSILESGLRFGDMDIFHRHESMAGNGEVLFSMANALKPGTFDL
                     DDIDHFSTRAVSFFLGLPGPRHPKQAFDLMVAAARKLAHELGGELKDEQRSVMTAQTI
                     EHYRQRIVEFERRALMNKR"
     gene            complement(1246..4734)
                     /gene="smc"
                     /locus_tag="VIO83_07210"
     CDS             complement(1246..4734)
                     /gene="smc"
                     /locus_tag="VIO83_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY8331098.1"
                     /translation="MRLKCIRLAGFKSFVDPTTVSFPSNMAAVVGPNGCGKSNIIDAV
                     RWVMGESSAKNLRGESMTDVIFNGSNTRKPVSQASIELVFDNSDGSLLGEYAAYSEIS
                     IRRRVTRDAQNTYFLNGTKCRRRDITDIFLGTGLGPRSYSIIEQGMISKLIEARPEDL
                     RNFIEEAAGISKYKERRRETESRIRRTQENLERLADLRQELERQLERLQRQAQAAEKY
                     QQFKAEERQLKAGLAALRWLALDELARQRQSVIGDQEVAMEALVAEQRSADASIERLR
                     DGHHDLSEAFNQVQARFYSVGGDIARIEQSIQHGQQRLRQLQDDLREAERAREETASH
                     LAHDRELLTSLAEELALLEPEQALAQAAAEEGAAALEEAEGAMQAWQEQWDGFNQRSA
                     EPRRQAEVQQARIQQLEQSLERLVERERRLQDERRQLADDPAAAQMEEFAEQLAEGEL
                     ELEGLQAGEEQLAERLQQLRDELQQAGREQQQAQGELQRLNGRIASLEALQQAALDPG
                     AGAAEWLREHELTARPRLAEGLRVDAGWELAVETVLGADLQAVLLDDFAGLALTAFTQ
                     GELRLACLDAGVARASGSLLDKVQAPVDLSPWLGRVRAVESLDEALAARSSLAEGESL
                     ISRDGYWVGRHFLRVRRASEAQSGVLARGQELERLAGEREVLEATLAALEERLQQLRE
                     QQTRQEDAREQQRRRLQEENRRQGELKARLSASQARHEQLALRRRRLEEELAEQAEQR
                     ALEVEQLGESRLYLQDALDAMASDTEQREQLLVARDALRERLDRVRQDSRQHKDHAHQ
                     LAVRLGSLRAQHDSTRQALERLELQAARLVERCEQLSLNLEEGDEPLQELRFRLEELL
                     ERRMQVDEEMRLARLALDDADRELRDAEKRRSSAEQQAQLLRGQLEQQRLDWQTLSVR
                     RKTLEDQLLEDGYDLPTVLANLPEAAAEREWESQLEQLAARIQRLGPINLAAIDEYQQ
                     QSERKRYLDAQNDDLVEALETLENVIRKIDKETRNRFKETFEQINGGLQALFPKVFGG
                     GQAYLELTGEDLLDTGVAIMARPPGKKNSTIHLLSGGEKALTALALVFSIFQLNPAPF
                     CMLDEVDAPLDDANVGRYARLVKEMSEKVQFIYITHNKIAMEMADQLMGVTMHEPGCS
                     RLVAVNVEEAVALAEA"
     gene            5035..5868
                     /locus_tag="VIO83_07215"
     CDS             5035..5868
                     /locus_tag="VIO83_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019471469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEY8331099.1"
                     /translation="MTQPSPPQSWRERLYVIIFKADTPAGKRFDEILLLAILASLAVV
                     MLDSVKSINQHYALPLVALEWSFTALFAVEYLLRLYCSPKPLRYAFSFYGVVDLLAVL
                     PALLALVYTGAQYLMIVRVLRMLRVFRILKLTPYLSQANFLLVALRGSQQKITVFLLT
                     VSTLVIVFGTLMYVVEGPHHGFTSIPTSIYWAVVTLTTVGFGDITPKTPLGQGLATLI
                     MITGYSIIAVPTGIFTAELASAMRGGSETLQHACPTCGKAEHEASAGFCSRCGHPLFP
                     K"
     gene            5954..6949
                     /locus_tag="VIO83_07220"
     CDS             5954..6949
                     /locus_tag="VIO83_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8331100.1"
                     /translation="MKRLFSASLLAASLLAAGAAQAATLLNVSYDVMRDFYKDYNPAF
                     QKHWQAEGGKPLTIQMSHGGSSKQARAVIDGLPADVITMNMATDINALHDHGQLIPQD
                     WAARLPDNSAPFTSATVFIVRKGNPKGLKDWPDLLKSGVEVVVPNPKTSGNGRYTYLS
                     AWGYVLKNGGDEQAAKDFVGKLFKQAPVLDTGGRAATTTFMQNQIGDVLITFENEAEM
                     IAREFGRGGFEVIYPSVSAEAEPPVAVVDKVVDKKGTRKEAEAYLKYLWSDEAQRIAA
                     NNYLRPRNPAILAEFADRFPKVDILPVVKTFGEWPQIQKTHFNDGGVFDQVYTAQ"
     gene            complement(7024..7968)
                     /locus_tag="VIO83_07225"
     CDS             complement(7024..7968)
                     /locus_tag="VIO83_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331101.1"
                     /translation="MRRFAVGILIALILPLTACTVTAGDPGPYYGRPYPPPAHGQWRW
                     DDDLRVYVSIGYPYVYYHDHSYYRWHDNHWSSGPRYNGPWRVVEHRYVPSRLGQRYYP
                     GYPGRRFDDSHDRGYDRYDRRDDDRWQQRDNRDLRNYQNRRDQQQREMERNRGDNRWQ
                     QRDNRDLRDYQNRRDLQQRDMGRNRGDSRGEHWDSRDLRAAPNRRELQQRDMQHNRGD
                     NRWERHDAPQNRQSQRHDGRQGEQWQQRDQRGNERRIEQRSQQRSERHAEQRPQGREE
                     DQQRTRRALMQSNPRDRQDAAGSRGGEQHQEYRGGRQW"
     gene            8214..9527
                     /gene="guaD"
                     /locus_tag="VIO83_07230"
     CDS             8214..9527
                     /gene="guaD"
                     /locus_tag="VIO83_07230"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HEY8331102.1"
                     /translation="MTAPLHAYRTALLYSLDDPARVGVESSYRYLQDGLLLVEAGRIR
                     AIESAANLLPQLPAGTPLRHWPDALIVPGFVDTHIHYPQTGIVGAGGEQLLDWLARYT
                     YPAELRFADRDYAEQVARFFVAELLRNGTTSALVFPTVHPESVDAFFTVAAELDLRML
                     CGKVLMDRNAPPALCDTAQSGYEQSLALIERWHGKGRLGYAVTPRFAASCSDAQLAMA
                     GRLLREYPGVHLHTHLAENRQEVAWVRTLFPARSSYLDVYAHHDLVGPRSVFAHAIHL
                     GDRDCRQLAASGAAVAFCPTSNLFLGSGLFDLGRAERLGLKVGIGSDIGAGTSFSLLQ
                     TLNEAYKVEQLQGRTLDPFKALYLATLGGARALGLDERIGSLAPGHEADFVVLDWHAT
                     PLLQFRLQQAQSLAERLAVLLTLGDDRVVRETFVAGHSRHRRDAN"
     gene            9617..9829
                     /locus_tag="VIO83_07235"
     CDS             9617..9829
                     /locus_tag="VIO83_07235"
                     /inference="COORDINATES: protein motif:HMM:NF025156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEY8331103.1"
                     /translation="MKEYKVTLYREGMLGSLLLGASKVDPEKFSDFLNQNAREGWRVV
                     TMEKDVRRMLLLWKREAYLVIMERDR"
     gene            9826..10038
                     /locus_tag="VIO83_07240"
     CDS             9826..10038
                     /locus_tag="VIO83_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331104.1"
                     /translation="MRAGALVCLLVFVGWVALALVQLWFAPLSGELFFKLTVTAGALF
                     IVALAITLVVNEYGRNKRLKDGGYLD"
     gene            complement(10169..>10600)
                     /locus_tag="VIO83_07245"
     CDS             complement(10169..>10600)
                     /locus_tag="VIO83_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEY8331105.1"
                     /translation="EPIQGGIARAREQLAARGESMPEPPPPAAQLAIKVSVSLAPELA
                     AKVQPDDTLFIFARAASGPPMPLAAKRLKVADLPVEVSLSDADAMMPQLKLSNFAEIR
                     LVARVSRAGTPTAGEWVGQSEVLKTAGVGDQKLVIDRADQK"
BASE COUNT         1684 a         3726 c         3357 g         1833 t
ORIGIN      
        1 gcatccggca cactcggctc gtcgagcacg taatagcggt agttgtgcgc gttgatggcg
       61 gtgcgcaatt gttcgatgcg ccgggcagcg ttactggtca taagaggcaa tccgtcaaac
      121 aaaaaaagca gccggggctg ctctgtttgc actgtctgtt cgcaagacgg ccccgcgtca
      181 ggcgcagggc cgctttatct ggttcaaata ctcagcgttt gttcatcagc gcccggcgct
      241 cgaactcgac gatacgctgg cggtaatgct cgatagtctg cgcggtcatt acactgcgct
      301 gctcgtcctt cagttcgcca cccagctcgt gagccaactt gcgcgcggca gcgaccatca
      361 ggtcgaaggc ctgcttggga tggcgcgggc ccggcaggcc gaggaagaag ctcaccgcac
      421 gggtactgaa gtggtcgatg tcgtccagat cgaaggtgcc gggcttgagg gcgttggcca
      481 tggagaacag cacctcgccg ttgccggcca tgctttcgtg gcgatggaag atgtccatgt
      541 cgccgaagcg caggccgctt tccagaatgc tctgcagcag cgccggaccc ttgaaaccgt
      601 cggggtcgcg ggcaaccacg ctgatcacca gcacttcctc caccggcggc aggtctcggg
      661 gcggcgcaga ggccgccgcg accttgtcgg ccttctcggt cgccttctcg gtcgccttct
      721 cgcgcagctt gtccttgctg cgcggctccg caggcatgtc gtcagccgcc ggcatgcgca
      781 gcggcgcggc caccggctcg gtcatgccga gctccagatc gcccgccagg ggctcgtcgc
      841 gacgcttgct gcgcggctcc agcgcggctt ccggcagatc atcctcgtcc agcgagggct
      901 cctggtgcct gtcgaccacg cgcacggggc ccagcaactc cttatcgcca tcatcgtcag
      961 gcagattggc ggcgctgcgg tcgagcttga acttcagacg gcccctgccc ccgcgcatgc
     1021 ggcgccagcc atcaatcagg attccgccga tcacaataat accgatgacg atcagccatt
     1081 cgcgcaaacc gatgtccatg aaagacacat tcccctatcc gaatccgggc aaatcaattg
     1141 caagttggca agcacagcgg ccggctcgaa ccgtttatat accgcaacgc cttgtaatgg
     1201 cccataaatt aacatgacga cacaaactat acaccgttcg tattgtcagg cttccgccaa
     1261 agccaccgct tcttcgacgt ttaccgctac cagacgcgaa cagcctggct cgtgcatggt
     1321 cacgcccatc agctgatctg ccatttccat ggcgatcttg ttgtgggtga tatagatgaa
     1381 ctgcaccttc tccgacattt ccttgacgag ccgtgcataa cggccaacgt tggcatcgtc
     1441 cagcggcgca tccacttcgt cgagcatgca gaacggcgcc ggattgagct ggaagatcga
     1501 aaacaccagg gccagcgccg tcagcgcctt ctcgccaccg gacagcagat ggatggtgct
     1561 gttcttcttg cctggcgggc gcgccatgat cgccacccct gtatcgagta gatcttcgcc
     1621 ggtaagttcc agatacgcct ggccgccgcc gaacaccttg ggaaacagcg cctgcagacc
     1681 gccattgatc tgctcgaagg tttccttgaa gcgattgcgg gtttccttgt cgatcttgcg
     1741 gatgacgttt tccagggttt ctagcgcctc gaccagatcg tcgttctgcg catccaggta
     1801 acgcttgcgc tcggactgct gctggtactc gtcgatggcg gcgaggttga tcggccccag
     1861 acgctggatg cgtgccgcca gctgctccag ctggctttcc cactcgcgct cggcagccgc
     1921 ctcgggcaga ttggccagca ccgtgggcag gtcatagcca tcctcgagca gctgatcctc
     1981 gagggtcttg cgccgcacgc tcaaggtctg ccagtcgaga cgctgctgct cgagctggcc
     2041 acgcagcagc tgggcctgct gctcggcgct gctgcggcgc ttctcggcgt cgcgcagctc
     2101 gcggtcggca tcgtcgagcg ccaggcgcgc cagacgcatt tcctcgtcga cctgcatgcg
     2161 ccgctcgagc agctcctcga gtcggaaacg cagttcctgc aggggctcgt cgccttcctc
     2221 gaggttgagg ctgagctgct cgcagcgctc gaccagacgc gccgcctgca gctcgaggcg
     2281 ctccagcgcc tgacgggtgg agtcgtgctg ggcgcgcagc gagccgaggc gcacggccag
     2341 ctggtgggcg tgatccttgt gctggcgact gtcctggcgc acgcggtcga ggcgctcgcg
     2401 caaggcgtca cgggcgacca gcagctgctc gcgctgctcg gtgtcgctgg ccatggcatc
     2461 cagcgcatcc tgcaggtaca ggcgcgactc gcccagctgc tccacctcca gggcgcgctg
     2521 ctcggcctgc tcggccagtt cctcctcgag gcgacggcga cgcagggcca gctgctcatg
     2581 gcgcgcctgg ctggcggaga gacgggcctt gagctcgccc tggcggcggt tctcttcctg
     2641 caggcgtcgg cgctgctgct cgcgcgcgtc ttcctgccgg gtctgctgct cgcgcagttg
     2701 ctgcaggcgc tcttccaatg ccgccagcgt cgcctccaac acttcgcgct cgcccgccag
     2761 ccgctccagc tcctggccgc gcgccagcac accgctttgc gcctcgctgg ctcggcgtac
     2821 gcgcaggaaa tgccggccga cccagtagcc gtcgcgactg atcagacttt cgccttcggc
     2881 caggctgctg cgcgcggcca gggcctcgtc cagcgactcg accgcacgca cgcggccgag
     2941 ccagggactc agatcgaccg gcgcctgcac cttgtccagc aggctgccgc tcgcacgggc
     3001 cacgccggca tcgagacagg ccaggcgcag ctcgccctgg gtaaacgcgg tcaaggccag
     3061 gccggcgaaa tcgtccagca gcaccgcctg caggtcggcg cccagcacgg tttccaccgc
     3121 cagctcccaa ccggcgtcga cgcgcaggcc ttcggccagg cgtggccgcg cagtcaactc
     3181 atgctcgcgc agccactcgg ccgcgccggc gccggggtcc agcgccgcct gctgcagcgc
     3241 ttccagcgag gcgatacggc cgttcagcct ctgcaactcg ccctgggcct gctgctgctc
     3301 gcgaccggcc tgctgcaact cgtcgcgcag ctgctgcaga cgctcggcca gctgctcctc
     3361 gccagcctgc agaccttcca gctccagctc gccctcggcc agctgctcgg cgaactcctc
     3421 catctgcgcc gcggccggat cgtcggccag ctggcgacgc tcgtcctgca ggcgccgctc
     3481 gcgctcgacc agccgttcga ggctctgctc cagctgctgg atgcgcgcct gctgcacctc
     3541 ggcctggcgc cgcggctcgg cgctgcgctg gttgaagccg tcccactgct cctgccaggc
     3601 ctgcatggcg ccttccgcct cctcgagagc ggcggcgccc tcctcggcgg cggcctgcgc
     3661 cagcgcctgc tcgggctcga gcagcgccag ctcctcggcc aggctggtca gcagctcgcg
     3721 gtcatgggcc aggtggctgg cggtttcctc gcgggcacgc tcggcctcgc gcagatcgtc
     3781 ctgcaactga cgcaggcgct gctggccgtg ctggatgctc tgctcgatgc gggcaatgtc
     3841 gccgcctact gaatagaagc gcgcctgtac ctggttgaag gcttccgaca ggtcatggtg
     3901 gccgtcgcgc agccgctcga tgctggcgtc ggcactgcgc tgctcggcga ccagcgcctc
     3961 catggccact tcctggtcgc cgatcacgct ctggcgctgc cgcgccagct cgtccagcgc
     4021 cagccagcgc agggcggcca gcccggcctt gagctggcgc tcctcggcct tgaactgctg
     4081 gtacttctcg gcggcctggg cctggcgctg cagacgctcg agctggcgct ccagctcctg
     4141 gcgcaggtcg gcgaggcgct cgaggttttc ctgggtgcgg cggatgcggc tctcggtctc
     4201 gcggcggcgc tccttgtact tggagatgcc tgcggcttcc tcgatgaagt tgcgtagatc
     4261 ctcggggcgc gcctcgatca gcttggagat catcccctgc tcgatgatcg agtagctgcg
     4321 cgggcccagg ccggtgccga ggaagatgtc ggtgatgtcg cgccgccggc acttggtgcc
     4381 gttgaggaag taggtgttct gcgcgtcgcg ggtcacccgg cggcggatgg agatctcgct
     4441 gtaggcggcg tactcgccaa gcaggctgcc gtcgctgttg tcgaagacca gctcgatgga
     4501 ggcctggctg accggcttgc gcgtgttgga gccgttgaag atgacgtcgg tcatcgactc
     4561 gccgcggagg ttcttcgccg aactctcgcc catcacccag cgcacggcgt cgatgatgtt
     4621 ggacttgccg caaccgttgg ggccgaccac cgccgccatg ttgctcggga agctgaccgt
     4681 ggtcgggtcg acgaacgact tgaagcccgc cagacggatg cacttcagac gcatggcacg
     4741 acactccggg cgcgactcga ccgcagcgcg aggcacggct gcagacagcg gaaattcggg
     4801 gtggcgccgg tggcggactg gaggatcatg ggcaggcgtc tgtcacgggt tgcgcggcgc
     4861 ggcagtctac cacagggcct tgccgctcca agcggagcgc cgtcgcgctt tgcctgcgcc
     4921 gtcacaggca cgcctcgccg gggcgcgcac gcggcggcac aaagcccgct aacataggcg
     4981 aaatgaacct ggacagccgc gcggcccgac ccgcgcctgg atcgagaagc ccccatgacg
     5041 caaccgtcac ccccgcagag ttggcgcgaa cgcctgtatg tcatcatctt caaggccgat
     5101 accccggccg ggaagcgctt cgacgaaatt ctcctgctgg ccatcctcgc cagtctggcc
     5161 gtggtgatgc tcgacagcgt caagtcgatc aaccagcact acgcgctgcc gctggtcgct
     5221 ctggaatgga gcttcaccgc gctgttcgcc gtcgaatacc tgctgcgcct gtactgctcg
     5281 cccaagccgc tgcgctatgc cttcagcttc tacggggtgg tcgacttgct cgccgtgctg
     5341 ccggcgctgc tggcgctggt ctacaccggc gcccagtacc tgatgatcgt ccgcgtgctg
     5401 cgcatgttgc gggtgttccg catcctcaag ctgacgccct acctgagcca ggccaacttc
     5461 ctgctggtcg cgctgcgcgg tagccagcag aagatcaccg tcttcctgct gaccgtgagc
     5521 accctggtca tcgtgttcgg caccctgatg tacgtggtcg agggaccgca ccacggtttc
     5581 accagcatcc ccaccagcat ctactgggcg gtggtgaccc tcaccaccgt cggcttcggc
     5641 gacatcacgc cgaagacccc gctcggccag ggcctggcca cgctgatcat gatcaccggc
     5701 tattcgatca tcgccgtacc caccggcatc ttcaccgccg aactggccag cgccatgcgg
     5761 ggcggcagtg aaactctgca gcatgcctgc ccgacctgcg gcaaagcgga acatgaagcc
     5821 agcgccggct tctgcagtcg ctgcggccat ccactgttcc cgaagtaata aacaaatacc
     5881 cataaagtat taattggaat aaaaatcggc agttatagtt ccgggataat caccccgcct
     5941 tgaggacatg cccgtgaaac gactgttcag tgcctccctg ctggccgcct ccctgctggc
     6001 ggccggcgcc gctcaggccg cgaccctgct caacgtctcc tacgacgtga tgcgcgactt
     6061 ctacaaggac tacaacccgg cgttccagaa gcactggcag gccgagggcg gcaaaccgct
     6121 caccatccag atgtcccacg gcggctcgag caagcaggcc cgcgcggtga tcgatggcct
     6181 gccggccgac gtgatcacca tgaacatggc caccgacatc aacgccctgc acgaccacgg
     6241 ccagctgatc ccgcaggact gggcggcccg cctgccggac aacagcgcgc ccttcacctc
     6301 ggccaccgtg ttcatcgtgc gcaagggcaa ccccaagggc ctgaaggact ggccggacct
     6361 gctcaagagc ggcgtcgaag tggtggtgcc caacccgaag acctcgggca acggccgcta
     6421 cacctacctg tcggcctggg gctacgtgct gaagaacggc ggtgacgaac aggctgccaa
     6481 ggacttcgtc ggcaagctgt tcaagcaggc accggtgctg gacaccggcg gtcgcgccgc
     6541 caccaccacc ttcatgcaga accagatcgg cgatgtgctg atcaccttcg agaacgaagc
     6601 cgagatgatc gcccgcgaat tcggccgcgg cggcttcgaa gtgatctacc cgagcgtgtc
     6661 cgccgaggcg gagccgcccg tggcggtggt cgacaaggtg gtcgacaaga aaggcacccg
     6721 caaggaggcc gaggcctacc tgaagtacct gtggtcggac gaggcccagc gcatcgccgc
     6781 caacaactat ctgcgcccgc gcaacccggc gatcctcgcc gagttcgccg accgcttccc
     6841 gaaagtggat atcctgccgg tggtgaagac cttcggcgag tggccgcaga tccagaagac
     6901 ccacttcaac gacggcggcg tgttcgacca ggtctatacc gcgcaataac gctgacgtga
     6961 acaaaaaggc gctgcggttt atccgcagcg cctttttcgt ttatccggcc acgccgcacg
     7021 atgctaccac tggcgcccgc cccggtactc ttggtgctgc tcgccgccac gggagcccgc
     7081 agcatcctgt cgatcgcgcg gattcgactg catcagcgca cgccgggtgc gctgctgatc
     7141 ctcctcgcgc ccctgcggac gctgctcggc atgccgctcc gagcgctgtt gggaacgctg
     7201 ttcgatgcgc cgctcgttac cccgctgatc gcgctgctgc cattgctcgc cctgacgccc
     7261 atcatgacgt tgcgactggc gattctgcgg agcgtcatgg cgctcccagc ggttgtcacc
     7321 gcggttgtgc tgcatgtcgc gctgctgcag ctcgcgccga ttcggggcgg cgcgcagatc
     7381 gcgactgtcc cagtgctccc cgcggctgtc gccacgattg cgacccatgt cgcgctgctg
     7441 cagatcgcgc cgattctggt aatcgcgcag gtcgcggttg tcccgctgct gccagcggtt
     7501 gtcgccacga ttgcgctcca tctcgcgctg ctgctgatcg cgtcgattct ggtaattgcg
     7561 caggtcgcga ttgtcccgct gctgccagcg gtcgtcgtcg cggcggtcgt agcggtcata
     7621 accgcgatcg tgcgagtcgt cgaaacgccg gcccgggtaa cccgggtagt aacgctgccc
     7681 cagccgcgac ggtacgtagc gatgctcgac tacccgccac gggccgttgt agcgcggccc
     7741 cgagctccag tgattgtcat gccagcgata ataggaatgg tcgtggtagt agacgtacgg
     7801 gtaaccgatg ctgacgtaca cacgcagatc gtcgtcccag cgccactgac catgagcggg
     7861 cggcgggtaa ggcctgccgt agtacgggcc gggatctccc gccgtaacgg tgcaggctgt
     7921 cagcggcagg atcagcgcga taaggatccc tacagcaaaa cgcctcattt gctagtctcc
     7981 acagcaggaa agaacccgct caaagcgttg attcgcggca cccgcaccaa tcatcgcgac
     8041 tgccagtgtt gctctcttgt gcgcttggcg cgccagggga ggaaggtacg cataacccgg
     8101 cagcgaaact acacggcggc gtcacaaagt cagacaacac gccaggggac ttgttgcaac
     8161 cacagccggt caaccgcaac actgtcccag agatcaccag gcagcccgcc cgcatgaccg
     8221 ccccactgca cgcctaccgc accgccctgc tgtacagcct ggatgacccg gcccgggtcg
     8281 gtgtcgaatc cagctaccgc tatctgcagg atggcctgtt gctggtcgag gccggccgca
     8341 tccgcgccat cgagagcgcc gcgaacctgc tgccacagct gccggccggc accccgctgc
     8401 ggcactggcc cgacgcgctg attgtccccg gctttgtcga cacccacatc cactacccgc
     8461 agaccggcat cgtcggcgcc ggcggcgagc agctgctgga ctggctggca cgctacacct
     8521 atccggccga actgcgcttc gccgaccgcg actacgccga acaggtggcc cgcttcttcg
     8581 tcgccgagct gctgcgcaac ggtaccacca gcgccctggt gtttcccacc gtgcaccccg
     8641 agtcggtgga tgccttcttc acggtcgccg ccgaactgga cctgcgcatg ctctgcggca
     8701 aggtgctgat ggaccgcaac gccccgccgg cgctgtgcga caccgcacag agcggctacg
     8761 agcagagcct ggcgctgatc gagcgctggc acggcaaggg ccgcctcggt tacgcggtga
     8821 cgccgcgctt cgccgccagc tgcagcgacg cgcagctggc gatggccggg cgcctgctgc
     8881 gcgagtatcc cggcgtgcat ctgcacaccc atctggccga gaaccgccag gaagtcgcct
     8941 gggtgcgcac gctgttcccc gcgcgcagca gctacctgga cgtctacgcc caccacgacc
     9001 tggtcggccc gcgctcggtg ttcgcccacg ccatccatct cggcgaccgc gactgccggc
     9061 agctggccgc cagcggcgcc gcggtggcct tctgtcccac ctccaacctg ttcctcggca
     9121 gcggcctgtt cgacctcggc cgcgcagaac ggctgggcct gaaggtcggc atcggcagcg
     9181 acatcggcgc cggcaccagc ttctcgctgc tgcagacgct gaacgaggcg tacaaggtgg
     9241 agcaattgca gggccgcacc ctcgacccct tcaaggcgct gtacctggcc accctcggcg
     9301 gcgcccgcgc actcggcctg gacgagcgga tcggcagcct cgcccccggt cacgaggccg
     9361 atttcgtggt gctggactgg catgccacgc cgctgctgca gttccgcctg cagcaggcgc
     9421 agagcctcgc cgaacgcctg gccgtgctgt tgaccctcgg cgatgaccgc gtggtgcggg
     9481 aaaccttcgt cgccggacac tcgcggcatc ggcgcgatgc gaactgagct gtagccgctt
     9541 tcgcaacaag cgccctccgc tatcatggcc gcgcgcagca caccggctgc ccgctcacca
     9601 caaccaggac aatacgatga aggaatacaa ggtcaccctg taccgggaag gcatgctcgg
     9661 ctcgctgctg ctcggcgcct ccaaggtcga cccggagaag ttcagcgact ttctcaacca
     9721 gaacgcccgc gaaggctggc gggtggtgac catggaaaag gacgtccgcc gcatgctgtt
     9781 gctgtggaag cgcgaagcct atctggtgat catggagcgc gaccggtgag agccggcgcg
     9841 ctcgtctgcc tactcgtctt cgtcggctgg gtcgcgctgg cgctggtgca gctgtggttc
     9901 gccccgctga gcggcgagct gttcttcaag ctgaccgtca ccgccggggc gctgttcatc
     9961 gtcgccctcg ccatcaccct ggtggtgaac gagtacggcc gcaacaagag gctgaaggac
    10021 ggcggctatc tcgactgaac cccgccgccc gcagggtggg gcaggccgca ggccgtaacc
    10081 caccaaccgg ccgcaaggcc ggcctgcagc atgcctggcg gcatgagcgg cgggttacgc
    10141 cgccatgcgg cgcacccagc cggcctcgct acttctgatc ggcccgatcg atcaccagct
    10201 tctggtcgcc gaccccggcg gtcttgagga cctcgctctg cccgacccac tcgccggcag
    10261 tcggcgtgcc ggcacgggaa acccgcgcca ccaggcggat ctcggcgaag ttggacagct
    10321 tcaactgcgg catcatggca tcggcatcgg acaggctgac ctccaccggc aggtcggcga
    10381 ccttcagacg cttggcggcc agcggcatcg gcgggccgga ggcggcgcgg gcgaagatga
    10441 acagggtatc gtccggctgc accttggcgg ccagttccgg cgccaggctg acgctcacct
    10501 tgatggccag ctgcgccgcg ggcggcggcg gttcgggcat gctttcgccc cgcgcggcga
    10561 gctgctcgcg cgcgcgggcg atgccgccct ggatcggctc
//
LOCUS       DATCUN010000182         5814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_158035, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000182 DATCUN010000000
VERSION     DATCUN010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158035"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>5814
                     /locus_tag="VIO83_07250"
     CDS             <1..>5814
                     /locus_tag="VIO83_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY8331106.1"
                     /translation="AEALQQGAPAGLSFSIPQNHERTNFPLTLAATAENQLSINWSYQ
                     CSHFSREAIVRMSEHLAALLTAMVAAPQAALSELDLLTEVERTQQLLEWNPPFTAERN
                     PLCVHQLIEAQALIRPDATALIFSDLELSFDQLNRRANQLAHRLRALGIGPEERVGLA
                     MQRSPEMIIGLLAILKAGGAYVPLDPAYPAERLRYLMQDSGISLLVSQSWLREKLPLP
                     EGLPVLEIDREALEGMPDSNPVNLTCGENLAYLIYTSGSTGRPKGVSVAHGPLAMHCV
                     KIGELYGMTPDDRELQFASISFDGAHERWLTPLVFGSAVMPRDDELWSVERTCAEIEK
                     HGITIACFTPSYLGQIADFMGEAGRDLPIRSYTPCGEGMAKHAFDEVQQVLQPKRLIN
                     GYGPTETVITPLIWLAYPETEFDSAFMPIGRPVGNRSAYILDSGLQPLPVGVAGELYL
                     GGEGVARGYHQRPDLTAERFVPDPFVPGARLYRSGDLARFRADGVVEYLGRIDQQVKI
                     RGFRIELGEIEACLLEHEGVREAFVIDRDGPVGRQLVGYVVPRDPLADEQELQDALTA
                     HLRSRLPAHMLPAHLLCLAALPLTPNGKLDRQGLPAPEATRHTQDQVAAASPAEALLV
                     DIWQDLLGLDSVGVTENFFELGGDSIISLQAVSRAGLRGLVFSPKQLFEQQSIRALAA
                     VASRREALLVEVPAVEKFSLVPHIDSTAREGLQDLYPLSPMQQGMLFHCLESPQLNPY
                     VNQLSVAVDGLQVPRFRAAWQALIQRHEVLRAAFRWRDGLADPLQAVFASVELPIEEL
                     DWREHSDTEKALGELAAAEQAKGFDLSCPPLMRFVLVRLGEERYQMIWIYHHLLLDGW
                     SASRLLGEMLRLYHHDSLPALTGRYADYIAWLQRQDQAQAESFWRERLASLEAPTILA
                     SASGAAGSGHGVMYSDLDAEATRKLHRFAKRQRVTLNTLVQGAWLLLLQRHSGQRSVA
                     FGATVAGRPTGLAGAQDMLGLFINTLPVIQTLDPHQPLGEWLRDLQDYNLAVRDFEHT
                     PLSDVQRWAGQGGQGLFDSIIVFENHPVDRALKGGEEGELRFAEVGSGGVTNFPMDLM
                     VSANDQGLEVEYLYLRDRFSEQEVQRIRAQLEGLLRALPEDADCLLGNIGLPEARVSL
                     PQAFSDNPDLLHSFNQHVRSQPQKIALFCEDREVSYADLEHQANALAQLLISRGIGPE
                     SLVAVALPRSERTIVAFLAVLKAGAAYLPLDLSYPAERLAYMLEDSAASLLLCDSDLG
                     DRLVLAASPPRVLLDQTSLVGNTECPVSHPLPGHLAYLIYTSGSTGQPKGVAVERGPI
                     ARHCRGIIDLYELAPRSRELHFMSFAFDGAQERWLSVLLAGGSLVIREDNLWTPEQTL
                     EVLHRHNVTVACFPPAYLQQMAEVAERLGNPPAVEVYCFGGDAVPDASFEQVKRALRP
                     QRLVNGYGPTETVVTPLLWRAQASETCEAAYAPIGRGVAGRGLYILDADLNPLPVGVS
                     GELYLGGECLARGYHQRPGMSAERFIADPFAAGGRMYRTGDLVRQRECGLIDYLGRLD
                     QQVKIRGFRIELGEIEARLRDCAGVQDAAVVVHDTPTGKQLVGYVVAAAAAAAGLDRR
                     LKAELQTQMPDYMVPARILVLQRFPLSANGKLDRRALPVPEWTLGGYRAPRNALEIAL
                     ATIWQEVLGVPQVGIDDNFFELGGDSLQVLKVISRVRSQPQLGFELKLRDLMQKPCIA
                     ELSGYQAAETSSAPDPLLALNGRVAKVPALFCLHAGFGTVFDYEPLARRLEGRRTVYG
                     LQCRMLLDPQWQDVSLLAMAQAYAQRIRTQQPQGPYYLLGWSLGGALTQLVAHELESQ
                     GQTVAFAGLVDSYVAGTAEAGDWREDLADFLKFVLGQPSEQAQALIALKAQGLNEREG
                     AAAVIEAAMHGANDHAALGASELTQIFATGSSLKQLAL"
BASE COUNT         1001 a         1855 c         1906 g         1052 t
ORIGIN      
        1 gccgaggcct tgcagcaggg cgcaccggct ggcttgagtt tctcgattcc acaaaaccac
       61 gagcgcacca acttcccgct gaccctggcg gccaccgcgg aaaaccaact cagcatcaac
      121 tggagctacc agtgcagcca cttcagccgc gaggcgatcg tgcgcatgag cgagcacctg
      181 gccgcgttgt tgacggcgat ggtggctgca ccacaagcgg ccttgagcga gctggacctg
      241 ctgaccgagg tggaacgcac gcagcaattg ctggagtgga acccgccgtt caccgcagaa
      301 agaaatccgc tgtgcgtgca tcaactgatc gaggcccagg cccttatccg acctgacgcc
      361 acggcgctga tcttcagtga cctcgaactg agcttcgacc aactcaaccg ccgcgccaac
      421 cagttggccc atcgcctgcg cgccttgggc atcggcccgg aagagcgggt cggcctggcc
      481 atgcagcgct cgccagagat gatcatcggc ctgttggcga tcctcaaggc cggcggtgcc
      541 tacgtgccgc tggacccggc ctacccggcg gagcgcctgc gctacctgat gcaagacagc
      601 ggcatttccc tgctggtcag ccaatcctgg ctgcgggaaa aactgccact gcccgaaggc
      661 ttgccggtgc tggaaatcga ccgcgaagcg cttgagggga tgcccgacag caacccggtc
      721 aacctgacct gtggcgaaaa cctcgcctac ctgatctata cctcgggttc caccgggcgg
      781 cccaaaggcg tcagcgtggc ccacggcccg ctggccatgc actgcgtaaa gatcggcgag
      841 ttgtacggca tgaccccgga cgatcgcgag ctgcaatttg cctcgatcag cttcgacggc
      901 gcccacgagc gctggctgac gcccctggtg ttcggttcgg cggtgatgcc gcgcgacgac
      961 gagttgtgga gcgtggagcg cacctgcgcc gagatcgaaa agcacggcat caccatcgcc
     1021 tgtttcaccc ccagctacct ggggcagatc gccgatttca tgggcgaagc gggacgtgat
     1081 ctgccgatcc gctcctacac cccatgcggt gaagggatgg ccaagcatgc gttcgacgaa
     1141 gtgcagcaag tcctgcaacc caagcgcctg atcaacggct atggcccgac cgaaacggtg
     1201 atcacgccgc tgatctggct ggcctatccg gagaccgaat tcgactcggc gttcatgccg
     1261 attggccggc cggtgggcaa ccgcagtgcc tacatcctcg acagcggttt gcagccgttg
     1321 ccggttggcg tggcgggtga gttgtacctg ggcggcgagg gcgttgcccg tgggtatcac
     1381 cagcgtcctg acctgactgc cgaacgcttc gtgcccgatc cgttcgtgcc gggggcgcgt
     1441 ctgtaccgca gtggcgacct ggcgcggttc cgtgccgatg gcgtcgtcga atacctcggc
     1501 cgtatcgacc agcaagtgaa gattcgcggc ttccgtatcg aactcggcga aatcgaagcc
     1561 tgcctgctcg aacatgaagg cgtgcgtgaa gcctttgtga ttgatcgcga cggtccggtg
     1621 ggccggcaac tggtgggcta cgtggtgccg cgcgatccgc tggctgacga acaggaactg
     1681 caggatgcat tgaccgcgca cctgcgcagt cgcttgccgg cgcacatgct gccagcgcat
     1741 ttgctatgcc tggccgcgtt gccgctgacg ccgaatggca agctcgatcg ccagggtttg
     1801 ccagcgcctg aggcgactcg acacactcag gatcaagtgg ccgccgcatc gcctgccgaa
     1861 gcgctgttgg tggacatctg gcaggacttg ctcggcctgg actcggtggg cgtcaccgag
     1921 aacttcttcg aactgggcgg tgattcgatc atctcgctgc aagcggtgag ccgtgccggg
     1981 ctgcgcgggc tggtgttcag tcccaagcag ttgttcgagc agcagagcat tcgtgcactg
     2041 gccgcggtgg cgagccgtcg tgaagccttg ttggtcgaag tgcctgcagt cgagaagttt
     2101 tccttggtgc cgcacatcga ttccaccgcc cgcgaaggct tgcaagacct gtatccgctg
     2161 tcgccgatgc agcagggcat gctgttccat tgcctcgaat cgccgcagct caacccctac
     2221 gtcaaccagt tgagcgtggc agtggacggc ttgcaggtgc cgcgattccg cgcggcgtgg
     2281 caagccttga tccagcgtca tgaagtcctg cgcgccgcgt tccgttggcg cgatggcctg
     2341 gccgatccgc tgcaagcggt gttcgccagc gtcgagttgc cgatcgagga actcgactgg
     2401 cgtgagcaca gcgacacaga aaaggcgctg ggcgaactgg ccgccgccga acaggccaag
     2461 ggtttcgacc tgagctgccc gccgctgatg cgctttgtcc tggtgcgcct gggtgaagaa
     2521 cggtaccaga tgatctggat ctaccatcac ctgctgctcg atggctggag tgcctcgcgc
     2581 ctgctcggcg agatgctgcg cctgtaccac cacgacagcc tgccggcgct gaccgggcgc
     2641 tatgccgact acatcgcctg gctgcaacgc caggaccagg cgcaggcaga aagcttctgg
     2701 cgtgagcgcc tggcgtcgct cgaggcaccg accatcctgg cctcggcctc gggtgccgca
     2761 ggttccggtc acggcgtgat gtacagtgac ctcgacgccg aggcgacgcg caagttgcac
     2821 cgtttcgcca agcgtcagcg cgtcaccctc aacaccctgg tgcaaggcgc ctggctgttg
     2881 ctgctgcaac gtcacagcgg ccagcgtagc gtggcgttcg gcgcgacagt ggcggggcgg
     2941 ccgacggggc tggccggtgc ccaggacatg ctcggcctgt tcatcaacac cttgccggtg
     3001 atccagaccc tcgacccgca ccagccgctg ggcgagtggc tgcgtgactt gcaggactac
     3061 aacctggccg tgcgcgactt cgaacacacg ccattgtccg acgtgcagcg ctgggccggg
     3121 cagggtggcc agggcctgtt cgacagcatc atcgtgtttg aaaaccatcc ggtagaccgt
     3181 gccttgaaag ggggcgagga aggtgagctg cgcttcgccg aagtcggcag cggcggcgtc
     3241 accaacttcc cgatggactt gatggtcagc gccaacgacc agggcctgga agtcgagtac
     3301 ctgtacctgc gagatcgctt cagtgaacag gaagtgcagc gcattcgcgc acagttggaa
     3361 ggtttgctgc gtgcgttgcc agaggatgcg gactgtctgc tcggtaacat cggcctgccc
     3421 gaagcgcgcg tgagcctgcc gcaagccttt agcgacaacc ctgatctgct gcacagcttc
     3481 aatcagcatg tgcgcagcca accgcagaaa atcgccttgt tctgtgagga tcgcgaagtc
     3541 agctacgccg atcttgaaca ccaggccaat gcgctggcgc agttgttgat cagtcgaggc
     3601 attggcccgg aaagcctggt ggcggtggcc ttgccccgtt cggagcgcac catcgtcgcg
     3661 ttccttgccg tgctcaaggc cggtgcggcc tatctgccgc tggacctgtc atacccggcc
     3721 gagcgcctgg cctacatgct cgaagattcg gccgccagcc tgttgctgtg cgacagcgac
     3781 ctgggggatc gcctggtact ggccgccagc ccgccacgcg tgttgcttga tcagacgagc
     3841 ctggttggca acaccgagtg cccggtttcc catccactgc cggggcacct ggcgtacctg
     3901 atctacacct ccggctccac cggccagccc aaaggcgtgg cggtggaacg tggaccgatt
     3961 gcccggcatt gtcgcggcat catcgacctc tacgaactgg cgccgcgcag ccgcgaactg
     4021 cacttcatgt cgttcgcctt cgacggtgcc caggaacgct ggcttagcgt gctgttggcc
     4081 ggtggcagcc tggtgattcg cgaggacaat ctgtggaccc cggaacagac cctggaggtg
     4141 ctgcatcgcc acaacgtcac cgtcgcctgc ttcccgccgg cgtacctgca acagatggcc
     4201 gaagtggccg agcgcctggg caatccgcca gcggtcgagg tgtactgctt tggcggtgat
     4261 gcggtgccgg acgccagctt cgagcaggtc aagcgcgccc tgcgtccgca gcgtctggtc
     4321 aacggctacg gcccgaccga aaccgtggtc acgccgctgc tgtggcgtgc gcaagccagc
     4381 gaaacctgcg aggcggccta tgcgccgatt ggccgtggtg tggcaggccg tggcctgtac
     4441 atcctcgacg ccgacctcaa tccgttgccg gtcggtgtca gcggcgagct gtacctgggc
     4501 ggcgagtgcc tggcccgtgg ttaccaccag cggccgggca tgagcgccga gcgtttcatt
     4561 gccgatccgt tcgcggccgg tggtcgcatg taccgcaccg gcgacctggt gcgccagcgc
     4621 gagtgtgggt tgatcgacta cctcggccgg ctcgaccagc aagtgaagat ccgcggcttc
     4681 cgtatcgaac tgggcgagat cgaagcccgt ctgcgtgatt gcgcaggggt gcaggatgcc
     4741 gccgtggtgg tgcatgacac gcccaccggc aaacagttgg tcggttatgt agtggccgcc
     4801 gctgctgccg ctgccgggct ggatcggcgc ttgaaggccg agctgcaaac gcagatgccg
     4861 gactacatgg tgccggcgcg gatcctggtg ctgcagcgct tcccgctgtc ggccaacggc
     4921 aagcttgatc gtcgcgcact gccggtgccg gaatggacgc ttggcggtta ccgcgcgcca
     4981 cgtaacgcgc tggaaatcgc cctggcgacg atctggcaag aggtattggg tgtgccgcaa
     5041 gtgggcatcg acgacaactt cttcgaactc ggcggcgact cattgcaggt gctcaaagtc
     5101 atctcgcggg tgcgtagcca gccacaactg ggcttcgaat tgaaactgcg cgacctgatg
     5161 cagaaaccct gcatcgctga actcagtggc taccaggctg ccgagacttc gagtgcgccg
     5221 gacccgttgc tggccttgaa tggccgggtg gcgaaggtgc cggcgctgtt ctgcctgcac
     5281 gccgggttcg gtacggtgtt cgactacgag cccctggccc gtcgcctgga aggccggcgt
     5341 acggtgtacg gcctgcaatg ccgcatgctg ctcgacccgc aatggcagga tgtgtcgttg
     5401 ctggccatgg cccaggccta tgcccagcgc attcgcacgc aacagccgca aggcccgtac
     5461 tacctgctcg gctggtcgct ggggggcgcg ttgacgcaat tggtcgccca tgaactggag
     5521 agccaggggc agacggtagc cttcgccggg ctggtcgaca gttatgtggc gggtaccgcc
     5581 gaagcggggg actggcgcga agatttggcg gacttcctga agtttgtcct cggtcaaccg
     5641 tctgaacaag cacaggcatt gattgccttg aaagcccaag gcctcaacga gcgcgaaggc
     5701 gccgccgcgg tgatcgaggc ggccatgcac ggcgctaatg accacgcagc actgggcgct
     5761 agtgaactga cgcagatatt cgccactggc tcgtcgctca aacaactcgc cttg
//
LOCUS       DATCUN010000183         3769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_26634, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000183 DATCUN010000000
VERSION     DATCUN010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26634"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VIO83_07255"
     CDS             complement(<1..216)
                     /locus_tag="VIO83_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HEY8331107.1"
                     /translation="MSTPSRTEWEHRASILKIESRAFIHGEYAAAVSGATFDCLSPID
                     GRLLAQVASCEGADAERAVLDARATFES"
     gene            406..1731
                     /locus_tag="VIO83_07260"
     CDS             406..1731
                     /locus_tag="VIO83_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY8331108.1"
                     /translation="MSEPSQPPAATPRLDDYWMPFTANRQFKASPRLLESAAGLYYRS
                     VDGRQILDGCAGLWCCNAGHGRREISEAVARQVATLDYAPSFQMGHPLPFELAERLAA
                     IAPPGLNKLFFTNSGSESADTALKIALAYQRAIGQGTRTRLIGRELGYHGVGFGGLSV
                     GGMLGNRRAFSANLLPGADHLPHTLDLERNAFSKGLPAFGAEKADELERLVALHGAEN
                     IAAVMVEPMSGSAGVILPPVGYLQRLREITRKHGILLIFDEVITGFGRIGEAFAAQRW
                     GVVPDILTSAKGLTNGAVPMGAVFVDEALFDAFMGGPDGIELFHGYTYSGHPLACAAA
                     LAALDIYQDEGLFQRAIALEDYWCDALLSLRDLPHVVDIRCVGLVGAVQLAADPAGAG
                     RRVFEQCFRDGALVRCTGDTLALSPPLIIEREQIDHLLEILAGALRQLA"
     gene            complement(1814..1969)
                     /gene="rpmG"
                     /locus_tag="VIO83_07265"
     CDS             complement(1814..1969)
                     /gene="rpmG"
                     /locus_tag="VIO83_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY8331109.1"
                     /translation="MRDLIRLVSSAGTGHFYTTDKNKRTTPGKIEIKKYDPVVRKHVI
                     YKEGKIK"
     gene            complement(1981..2217)
                     /gene="rpmB"
                     /locus_tag="VIO83_07270"
     CDS             complement(1981..2217)
                     /gene="rpmB"
                     /locus_tag="VIO83_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY8331110.1"
                     /translation="MSRVCQVTGKGPVTGNKISHANNKTRRRFLPNLQHHRFWVESEK
                     RFVRLRVSAKGMRVIDKRGIDAVLAEIRTRGEKV"
     gene            complement(2439..3113)
                     /gene="radC"
                     /locus_tag="VIO83_07275"
     CDS             complement(2439..3113)
                     /gene="radC"
                     /locus_tag="VIO83_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY8331111.1"
                     /translation="MSIRDWPVQERPREKLLARGAATLSDAELLAIFLRTGVAGRSAV
                     DLARHLLSEFGGLRQLLEADLEGFSAHLGLGPAKYAQLQAVLEMGRRHLAARIQRDSA
                     LESPQAVRDYLKARLRHEPHEVFACLFLDTRHRVLAFEVLFHGSIDGASVYPRQVVKR
                     ALAHNAAAVILTHNHPSGVAEPSQADRQLTQKLKEALALVEVRVLDHFVVGDGEPVSL
                     AEYGWI"
     gene            3311..>3769
                     /locus_tag="VIO83_07280"
     CDS             3311..>3769
                     /locus_tag="VIO83_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEY8331112.1"
                     /translation="MQRLQRKRIVLGIGGGIAAYKSAELVRRLRDQGAEVRVVMTRAA
                     REFITPLTLQALSGHPVFHDLLDPAAEAAMGHIELARWADLVLIAPATADLMARLAQG
                     SADDLLTTLVLATDAPVALAPAMNQAMWRDPATQANAELLAQRGLHLFGPA"
BASE COUNT          587 a         1342 c         1231 g          609 t
ORIGIN      
        1 ggactcgaag gtggcgcgcg catccagcac cgcccgctcg gcgtcggcgc cttcgcagct
       61 ggccacctgc gccagcaggc ggccgtcgat cgggctgagg cagtcgaaag tcgccccgga
      121 gacggccgcg gcgtactcgc catggatgaa ggcgcggctc tcgatcttca ggatgctggc
      181 gcggtgttcc cactcggtac ggctcggcgt gctcatgagg tgctccgggg ctgttgtttg
      241 ggtcatcatg gctgcagctg gaaaacctgc gcaagccggg gcaggcgaaa ttccgcgcga
      301 aacagctcag gatttttgac gaatcggcgc gcgagacggc atagcgttca ctatcctgca
      361 cacattgccc agccaccgcg cccttttgcg caggaagacc acgccatgag cgagccaagc
      421 cagccgcccg ccgccactcc gcgcctcgac gactactgga tgcccttcac cgccaaccgc
      481 cagttcaagg ccagcccgcg cctgctggaa agcgccgcgg ggctgtacta ccgcagcgtc
      541 gacggccggc agatcctcga cggctgcgcc gggctgtggt gctgcaacgc cggccacggc
      601 cgccgcgaga tcagcgaggc ggtggcgcgc caggtggcca cgctggacta cgcgccgagc
      661 ttccagatgg gccacccgct gccgttcgaa ctggccgaac gcctcgccgc catcgccccg
      721 ccggggctga acaagctgtt cttcaccaac tccggctcgg agtcggcgga caccgcgctg
      781 aagatcgccc tcgcctacca gcgcgccatc ggccagggca cgcgcacccg cctgatcggc
      841 cgcgagctgg gctaccacgg cgtcggcttc ggcggcctct cggtgggcgg catgctcggc
      901 aaccgccggg cgttctccgc caatctgctg ccgggggccg accacctgcc gcacaccctg
      961 gatcttgagc gcaatgcctt cagcaagggg ctgccggcgt tcggcgcgga aaaggccgac
     1021 gagctggaac gcctggtcgc cctgcacggt gcggagaaca tcgccgcggt gatggtcgag
     1081 ccgatgagcg gctcggccgg ggtgatcctg ccgcccgtgg gctacctgca gcgcctgcgc
     1141 gagatcaccc gcaagcacgg catcctgctg atcttcgacg aggtgatcac cggcttcggg
     1201 cgcatcggcg aggccttcgc cgcccagcgc tggggcgtgg tgccggacat cctcaccagc
     1261 gccaagggcc tgaccaacgg cgcggtgccg atgggcgcgg tgttcgtcga cgaggccctc
     1321 ttcgacgcct tcatgggcgg cccggacggc atcgagctgt tccacggcta cacctattcc
     1381 ggccacccgc tggcctgcgc cgccgccctc gcggcgctgg acatctatca ggacgagggc
     1441 ctgttccagc gcgccatcgc gctggaggac tactggtgcg acgcgctgct cagcctgcgc
     1501 gatctgccgc acgtcgtcga catccgctgc gtcggtctgg tcggcgccgt gcagctcgcc
     1561 gccgatccgg ccggcgccgg ccggcgggtg ttcgagcagt gcttccggga cggcgcgctg
     1621 gtgcgctgca ccggcgacac cctggcgctg tcgccgccgc tgatcatcga gcgggagcag
     1681 atcgaccacc tgctggagat cctcgccggc gccctgcgcc agctcgcctg agccgcagcg
     1741 acaaaacgca gaaaccgcag gcaagaaaaa acccgccacg aggggcgggt ttcttcttca
     1801 agggccggat caattacttg atcttgcctt ccttgtagat cacgtgcttg cggacgaccg
     1861 gatcgtactt cttgatctcg atcttaccgg gggtggtgcg cttgttcttg tcggtggtgt
     1921 agaagtggcc ggtaccggcg ctggacacca aacggatcag atcacgcatg attgtgctcc
     1981 ttaaactttt tcgccgcggg tgcggatttc agccagcacg gcgtcgatgc cgcgcttgtc
     2041 gatgacgcgc atgcccttgg ccgatacgcg cagacgcacg aagcgcttct cggactcgac
     2101 ccagaagcga tggtgctgca ggttcggcag gaaacgacga cgggttttgt tgttcgcgtg
     2161 ggaaattttg ttcccggtaa ccggaccctt accggtcact tgacagactc tcgacatacc
     2221 tcagccctct taaaccacat gcccaacccg gcatgggttg gccgcttgaa tcggtgtgaa
     2281 ttttactttc tatcggggtc ttaccgactg tgccatcggg ctgaaaagcc gggccccaga
     2341 aaagagcgca gttttatagc agaaagccgc cccagtcgca aggcgcacgc cacagagccg
     2401 accaccggcc ggaacagata tcccggccgc gtagtctctc agatccagcc gtattcggcg
     2461 agcgacaccg gttcgccgtc gccgaccacg aaatgatcga gcacgcgcac ctcgaccagc
     2521 gccagcgcct ccttgagctt ctgggtcagc tggcggtcgg cctggctcgg ctcggccacc
     2581 cccgagggat ggttgtgggt gaggatcacc gcggcggcgt tgtgcgccag cgcgcgcttg
     2641 accacctggc gcggatagac gctggcgccg tcgatggaac cgtggaacag cacctcgaag
     2701 gccagcacgc gatggcgggt gtcgaggaac aggcaggcga acacctcgtg cggctcgtgg
     2761 cgcaggcgcg ccttgaggta gtcgcgcacc gcctgcgggc tttccagcgc cgagtcgcgc
     2821 tggatccgcg cggccaggtg gcggcggccc atttccagca ccgcctgcaa ctgggcgtac
     2881 ttggccggcc ccaggcccag atgggcgctg aagccctcaa gatcagcctc cagcaactgg
     2941 cgcaggccgc cgaactcgct cagcaggtgg cgcgccagat ccactgcgct gcgcccggcc
     3001 accccggtgc gcaggaagat cgccagcagc tcggcatcgc tcaaggtcgc cgccccgcgg
     3061 gccaacagtt tttcccgcgg ccgctcctgc accggccagt cccgaatgct catccgtcct
     3121 ctccctgtgt gatggtcgcg cgccgtcctg gcgcagccgc gcgtttcacg ctgcaggccg
     3181 cgtcccacgc tgcctgcgcg agtggcgctg tgctatctta gccgccctgt tccgaagccg
     3241 gcagcgcctg ttgtgacccc gttcgcgctg ccgtgtattc caccccgttc accgaataaa
     3301 aggcaggttc atgcagcggc tgcagcgtaa acgcatcgtc ctgggtatcg gcggcggcat
     3361 cgccgcctac aagagcgccg agctggtccg ccgcctgcgc gaccagggcg ccgaagtgcg
     3421 cgtggtgatg acccgcgccg cccgcgaatt catcaccccg ctgaccctgc aggcgctgtc
     3481 cggccatccg gtgttccacg acctgctcga cccggccgcc gaggcggcca tgggccacat
     3541 cgagctggcc cgctgggccg acctggtact gatcgccccg gccaccgccg acctgatggc
     3601 gcgcctggcc cagggcagcg ccgacgacct gctgaccacc ctggtgctgg ccaccgacgc
     3661 gccggtggcg ctggccccgg cgatgaacca ggcgatgtgg cgcgacccgg ccacccaggc
     3721 caacgccgaa ctgctcgccc agcgcggcct gcacctgttc ggcccggcc
//
LOCUS       DATCUN010000184        30501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_177399, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000184 DATCUN010000000
VERSION     DATCUN010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 30501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30501
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177399"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VIO83_07285"
     CDS             complement(<1..266)
                     /locus_tag="VIO83_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8331113.1"
                     /translation="MLICPICSGALHTLDNGVQCSHGHRFDRARQGYLNLLPVQHKKS
                     LDPGDNAAMVEARRRFLDGGNYAPLAARLAELAAGRAPARWIDIG"
     gene            complement(270..1412)
                     /gene="dapE"
                     /locus_tag="VIO83_07290"
     CDS             complement(270..1412)
                     /gene="dapE"
                     /locus_tag="VIO83_07290"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY8331114.1"
                     /translation="MTPPSPTLELACELIRRASVTPLDEGCQSLMMQRLAAVGFNVEP
                     MQIEDVENFWARRGGEGPVLCFAGHTDVVPTGPLEAWQHPPFAAFVDADGMLCGRGAA
                     DMKGSLAAMIVAVERFVADHPQHQGAIAFLITSDEEGPALHGTQAVVERLAARNERLD
                     WCIVGEPSSTSLVGDMVKNGRRGSLNGKLRIQGVQGHVAYPHLAQNPIHLVAPALAEL
                     AAEHWDAGNAFFPPTSFQISNIHSGTGTTNVIPGELVALFNFRFSTESTVEGLQQRVA
                     AILDRHGLKWQVEWSLSGLPFLTEPGALLDAVSASIRNVTGRETVASTSGGTSDGRFI
                     ATLGTQVVELGPVNATIHQVNERVLASDLDLLTEIYYQTLVRLLAN"
     gene            1643..2872
                     /locus_tag="VIO83_07295"
     CDS             1643..2872
                     /locus_tag="VIO83_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY8331115.1"
                     /translation="MAKKQVNNKSSQLLDWLAANGGYNPVELTEDGVPLVGTSEDDLL
                     TGGAGDDVIDGGEGDFDTVNYGNAKKVKVDLAAETATGEGNDTLVNVENVIGSAKGSD
                     FITGDELGNMLDGWGGNDKLWGDAGDDILFGGDGNDQLRGGDGYDELDGGAGNDQLFG
                     DAGDDLLYGGAGNDQLRGGEGNDVLYGDAGNDTLRGEAGDDTFYAGAGNDSMFGGAGN
                     DTYYVEDAKDKVKENAGEGTDEIVASYSYSLEKVKNVENLTLSEDAKPLNINATGNSL
                     DNVLTGNSGDNVLKGLAGNDILVGREGDDTLIGGDGDDIFVFDNLDGVDSVEDFTIGS
                     DLLQLDALVFTSLAAGVQDANLVMGTEAADSDDFLVFDADNGALYYDADASGAEAAVQ
                     IATVGVSSLVAADFVVV"
     gene            complement(3060..5648)
                     /locus_tag="VIO83_07300"
     CDS             complement(3060..5648)
                     /locus_tag="VIO83_07300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8331116.1"
                     /translation="MSARKLGLHLVMVVAVASLFTGLWAWYNQPLKAPDWPDQISGFS
                     FSPFRLHQSPQGNTYPSEDEIRADLNLVATHTDNIRTYSVDGTLADIPRLAEEAGLRV
                     TLGIWLSNDLAANERQIERGIEIAKRERSVVRVIVGNEALFREEVTIEQMIGYLDRVR
                     SALKVPVTTAEQWHIWQKYPEMAKHVDLIAAHVLPYWEFVPREDSIQFVLDRARDMKK
                     LFPKKPLLLAEVGWPSNGRTRGGAEANQADQAIYLRTLVNKLNSKGYNYFVIEAFDQP
                     WKASEEGSVGAYWGVYNADRQAKFPLTGTVVEIPQWRLLAVASGVLALLALALLLIDG
                     SALRQRGRTFLTIVSFLAATMLVWIAYDYSQQYSNWFSTLVGLLLALGAVGVMIVLLT
                     EAHELAETVWKHERVRPFPPVTGDDAYRPKVSIHVPCYNEPPEMVKQTLDALANLDYP
                     DFEVLVIDNNTKDPAVWEPVQAHCATLGPRFRFFHVAPLAGFKGGALNYVLQHTAADA
                     EVIAVIDSDYCVERNWLKHMVPHFADPEIAVVQSPQDYRDQNESLFKKLCYAEYKGFF
                     HIGMVTRNDRDAIIQHGTMTMTRRSVLDELGWADWCITEDAELGLRVFEKGLSAAYSE
                     QSYGKGLMPDTFIDFKKQRFRWAYGAIQIMKRHLASLFMGKGSELSRGQRYHFIAGWM
                     PWIADGMNIFFTLGALLWSAAMIIVPHRVDPPLMIFAIPPLALFVFKLGKILFLYRRA
                     VGVNMRDAISAAVAGLALSHTIAKAVLYGFVTSSIPFFRTPKMRSNHGLLKAIAEARE
                     EVFVMLLLWGAAVGISLTQKNAGLDVTFWVIVLLVQSLPYLAALVMALLSSLPKPEED
                     AAEVPA"
     gene            complement(5892..6308)
                     /gene="tnpA"
                     /locus_tag="VIO83_07305"
     CDS             complement(5892..6308)
                     /gene="tnpA"
                     /locus_tag="VIO83_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEY8331117.1"
                     /translation="MENIKTPRTGRHCVSMMHCHLVFITKYREGLFTAEHLESLHSIF
                     AKVCEDFEAELVTFNGEKDHVHLLVNYPPKVSISALVNSLKGASSRRIKRLYPELNQP
                     AYRGNALWSPSYFAGSVGGASIEAVQEYIRARKTPD"
     gene            6330..7385
                     /locus_tag="VIO83_07310"
     CDS             6330..7385
                     /locus_tag="VIO83_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8331118.1"
                     /translation="MKMIKALRVRIRDKHAALLSEMARQVNFVWNYVNELSSRSIRER
                     GRFLSAYDLHPYLKGSGKLLGLHSQTIQGITEEYVTRRKQFKKQQLAWRRSGGSRRSL
                     GWIPFKAATWKDGQVVFNGHYFKVWDSYGLAQYRFRSGSFNEDARGRWYFNVVVEVDV
                     QPSPGQEALGIDLGLKDVATCSDGTKLDNARFYRGLEPKLAIAQRAGKKARVRAIHAK
                     IANRRKEALHQFSRALVERCGTIIVGDVSSTKLVKTSMAKSVLDAGWGQLKAMLAYKC
                     AHAGIVFKEVNEAYTTQTCSCCGVLPNSRPRGIAGLGIREWTCSECGVTHDRDINAAR
                     NILAAGHGRPAVGIPVI"
     gene            7432..8244
                     /gene="tcdA"
                     /locus_tag="VIO83_07315"
     CDS             7432..8244
                     /gene="tcdA"
                     /locus_tag="VIO83_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HEY8331119.1"
                     /translation="MHIDEQRFGGIARLYGRLGLERLAAAHVAVVGIGGVGSWAAEAL
                     ARSGVGEISLFDLDDVCVTNTNRQIHAHVGNIGRAKVEAMAERIRAINPDCIVHAVTD
                     YVTRETMADYITEQLDGVIDCIDSVVAKAALISWCKRRKIQIVTTGAAGGQLDPTQVQ
                     VADLNKTVNDPLVAKVRSLLRRDYNFSRTPGRTYSVACVYSREQLRYPQPDGSVCQAK
                     GFAGEGVRLDCAGGFGAVTMVTATFGMVAAARLVDKLVAGARRPAERKTIVG"
     gene            complement(8335..8736)
                     /locus_tag="VIO83_07320"
     CDS             complement(8335..8736)
                     /locus_tag="VIO83_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015634326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEY8331120.1"
                     /translation="MSLPGAASEALADFSHCPGWEQRARLLMQWGERLAPLSEAEQCE
                     GNRVHGCESRVWLVADGDDGRLNFRASSDARLLRGLLALLLLRVNGLTATELAEVDVA
                     DWFTQLGLSRQLSPSRSNGLNAVLQRIRELA"
     gene            complement(8733..9938)
                     /locus_tag="VIO83_07325"
     CDS             complement(8733..9938)
                     /locus_tag="VIO83_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEY8331121.1"
                     /translation="MSLPSPWRADFPAFAAFTASRQTYLDTAATAQKPQKVLDALLDH
                     YAGGAANVHRAQHGPGERATLAFEGAREKVAQWLNADSPAEIVFTRGSTEGLNLLAYG
                     LEHLFSAGDEIVISALEHHANLLPWQQLAQRRGLKLVVLPLDGAGDIDLEQAALLIGP
                     RTRLLAVSQLSNVLGRWQPLERLLPLARAQGAFTVVDGAQGAVHGRHDLQALDCDFYV
                     FSGHKLYAPDGIGALYGRSRALAQLRHWQFGGEMVQHADYHSARFRPAPLGFEAGTPA
                     IAAAIGLGAALDYLGELDEYAVAAHEAALHQRLLTGLRQFDGLQLLSEPHTALASFVV
                     DGVHNADLSHLLSEQGIAVRAGQHCAMPLLHSLGRPGALRVSLGLYSEGDDLERFFAA
                     LNHALELLR"
     gene            complement(10204..11238)
                     /gene="dapD"
                     /locus_tag="VIO83_07330"
     CDS             complement(10204..11238)
                     /gene="dapD"
                     /locus_tag="VIO83_07330"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY8331122.1"
                     /translation="MSQHLYSIAFGVGTQNRQGTWLEVFYALPLLNPPAELVATTANA
                     LGYTGGNQTLALSNNQAHDLAGALKQIDAAQAALLTRLAESRKPLVAVLLAEDSAPAS
                     TPEAYLKLHLLSHRLVKPHGTSLAGIFALLPNVAWTNEGAIDLAELAERQLEARLKGE
                     LLEVFSVDKFPKMTDYVVPSGVRIADTARVRLGAYVGEGTTIMHEGFVNFNAGTAGPG
                     MIEGRVSAGVFVGKGSDLGGGCSTMGTLSGGGNIVISVGEGCLIGANAGIGIPLGDRC
                     TVESGLYITAGTKVAVLDDQGSLVEVVKARDIAGQNDLLLRRNSQTGAVECKTNKTAV
                     ELNEVLHAHN"
     gene            complement(11280..11642)
                     /locus_tag="VIO83_07335"
     CDS             complement(11280..11642)
                     /locus_tag="VIO83_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HEY8331123.1"
                     /translation="MPSEIKDLQLYGIKACDTMKKARSWLDERQLSYRFHDYKAAGID
                     RASLERWCNEHGWDKVLNRAGTTFRKLADEQKADLDQARAIDLMLAQPSMIKRPVLDL
                     GERTLLGFKPELYAAALA"
     gene            complement(11681..11851)
                     /locus_tag="VIO83_07340"
     CDS             complement(11681..11851)
                     /locus_tag="VIO83_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331124.1"
                     /translation="MMDNPLNVMTALIGIGMLMLGVGYTQRASDAGVAVLAIGVLVML
                     SSLVYRLYLAFG"
     gene            complement(11848..13050)
                     /gene="dapC"
                     /locus_tag="VIO83_07345"
     CDS             complement(11848..13050)
                     /gene="dapC"
                     /locus_tag="VIO83_07345"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020296625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEY8331125.1"
                     /translation="MNRALDLLQPYPFEKLRALLAGAQPPATKKPIALSIGEPKHRSP
                     SFVAEALASNLDQLAVYPSTIGLPALRESIAAWCERRFGVPAGWLDPAKHVLPVNGTR
                     EALFAFTQAVVDRSPDALVVSPNPFYQIYEGATLLAGAQPHYLPCLAENGFNPDFDAV
                     PAEVWQRCQILFICSPGNPTGALVPLDTLKQLIALADQHDFVIAADECYSELYFDEQN
                     PPAGLLSACAELGRSDFKRCVVFHSLSKRSNLPGLRSGFVAGDAEILKPFLLYRTYHG
                     CAMPVQTQLASIAAWNDEAHVQANRALYREKFDAVLDILGGVLEVERPDGGFYLWAKT
                     PGDDTTFTRELFEREHVTVVPGSYLSRAVDGLNPGAGRVRMALVAPLAECVEAAERIR
                     AYLQERQG"
     gene            complement(13064..15775)
                     /locus_tag="VIO83_07350"
     CDS             complement(13064..15775)
                     /locus_tag="VIO83_07350"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY8331126.1"
                     /translation="MSHPEVDHELFDRGQFQAELALKSSPIAAFKKAIRRAGEILDGR
                     FSATCDIRRLITGRAWFVDQILREAWARFDWGDANDIALVAVGGYGRGELHPHSDIDI
                     LILLDGAQHDSLREPIEGFLTLLWDIGLEIGQSVRSVEDCAEQGRADLTVITNLMESR
                     TLVGADDLRRRMLAATGTQYMWPAREFFLAKRAEQHARHAKYNDTEYNLEPNVKGSPG
                     GLRDLQNILWVIGRYYGTLTLETLVTRGLLTPSEFGLLSASQEFLWKVRYALHMVAGR
                     AEDRLLFDHQRRLAGLLGFTASNAKLAVESFMQKYYRVVMAAAELSDLVNQTLEEQLL
                     EQPDSGELRPLNARFQVRDGFIEATDPGVFKRCPSAILEIFVLMAQQPEIRGVRSGTI
                     RLLRDHRQLIDDKFRHDIRNTSLFVELFKSHEGIHRNLRRMNRYGILGRYLPEFGHII
                     GQMQHDLFHIYTVDAHTLNLIKNLRKLDRPEMAEKYPLASELMAQLPKPELVYFAGLY
                     HDIAKGRGGDHSELGAKDALAFCERHHLPPWDSRLIAWLVKNHLVMSTTAQRKDLSDP
                     QVIHDFACTVGDQTHLDYLYVLTVADINATNPTLWNSWRASLMRQLYAETKRALRRGL
                     ENPIDREERIRENQHAALKLLHAREGIDADAAEQLWAQLGDHYFLRHSAEDIAWHAEA
                     ILRHPHDGAPLVLIKETTQREFEGGTQIFIYAPDQHDFFAVTVAAMDQLNLNIQDARI
                     LTSTSQFTLDTYVVLDADGGSIGDDPERIAEIRQGLVDALKNPLDYSTIIKRRVPRQL
                     KSFGFAPQVTIHTDAQRPLSVIEVIAPDRPGLLARVGQIFLDFDLSVQNAKIATLGER
                     VEDVFFVTDAHHQPLSDPELCARLQESLVRQLSDTQDSAVPLSRISF"
     gene            complement(15811..16593)
                     /gene="map"
                     /locus_tag="VIO83_07355"
     CDS             complement(15811..16593)
                     /gene="map"
                     /locus_tag="VIO83_07355"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY8331127.1"
                     /translation="MTVTIKTPEEIEKMRVAGRLAAEVLEMIEPHVRAGVTTEELNRL
                     CHDYIVDVQQAVPAPLNYHGFPKSICTSINHVVCHGIPNDKPLKDGDILNIDITVIKD
                     GYFGDTSKMFLVGNAPEWAQRLCQITQECLYKGISLVRPGAHLGDIGEIIQKHAESNG
                     YSVVREYCGHGIGNVFHEEPQVLHYGRAGSGLELKAGMTFTIEPMINQGRHETRLLGD
                     GWTAITKDRKLSAQYEHTLLVTENGCEILTLRNDDTIARCLP"
     gene            16860..17591
                     /gene="rpsB"
                     /locus_tag="VIO83_07360"
     CDS             16860..17591
                     /gene="rpsB"
                     /locus_tag="VIO83_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY8331128.1"
                     /translation="MSQVNMRDMLKAGCHFGHQTRYWNPKMGKFIFGSRNKIHIINLE
                     KTLPMFNEALAFVEKLAAGKNKILFVGTKRSASKIVREEAARAGMPFVDHRWLGGMLT
                     NFKTIRASIKRLRDLEVQSQDGTFEKLTKKEALMRTRDLEKLERSLGGIKDMGGLPDA
                     LFVVDVDHERIAITEANKLGIPVIGIVDTNSSPEGVDYIIPGNDDAIRAVQLYLGSMA
                     DAVLSGKSKSGAEFAEAASSEAAEG"
     gene            17718..18587
                     /gene="tsf"
                     /locus_tag="VIO83_07365"
     CDS             17718..18587
                     /gene="tsf"
                     /locus_tag="VIO83_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY8331129.1"
                     /translation="MAEITAALVKELRERTGQGMMDCKKALTAAGGDIEKAIDDMRAA
                     GAIKAAKKAGNIAAEGSIAVKVADDNKSAVILEVNSQTDFLALQDDFKNFVAASIEKA
                     FADKLADVAPLIAAQEPAREALVSKCGENVNIRRLTRVEGDVVGSYLHGHRIGVVVTL
                     KGGNAELAKEIAMHVAASNPQFVSAAEVSAEAVAKEKEIFLALNADKIAGKPENIVEN
                     MVKGRIAKFLAEASLVEQPFVKNPDVKVGELAKQAGAEVVSFVRYEVGEGIEKAEVDF
                     AAEVAAQVAASQQ"
     gene            18805..19539
                     /gene="pyrH"
                     /locus_tag="VIO83_07370"
     CDS             18805..19539
                     /gene="pyrH"
                     /locus_tag="VIO83_07370"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY8331130.1"
                     /translation="MAQLSGRQPRYKRILLKLSGEALMGSEEFGIDPKVLDRMALEIG
                     QLVGIGVQVGLVIGGGNLFRGAALSAAGMDRVTGDHMGMLATVMNSLAMRDALERSNI
                     PALVMSAISMVGVTDHYDRRKAVRHLASGEVVIFSAGTGNPFFTTDSAACLRGIEVNA
                     DLVLKATKVDGVYTADPFKDPNAEKFEHLTYDEVLDRKLGVMDLTAICLCRDHKMPLR
                     VFNMNKPGALLNIVVGGAEGTLVEEA"
     gene            19542..20099
                     /gene="frr"
                     /locus_tag="VIO83_07375"
     CDS             19542..20099
                     /gene="frr"
                     /locus_tag="VIO83_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY8331131.1"
                     /translation="MINEIKKDAQERMRKSLESLDHAFAKIRTGRAHPSILDSVMVSY
                     YGSDTPLRQVANVIAEDSRTLALTVFDKSMIQAVEKAIMTSDLGLNPATAGTTIRVPM
                     PALTEETRKGFTKQARAESENARVAIRNIRRDALAQLKDLVKEKEISEDDERRAADEV
                     QKLTDKAVADVEKALEAKEADLMAV"
     gene            20116..20880
                     /gene="uppS"
                     /locus_tag="VIO83_07380"
     CDS             20116..20880
                     /gene="uppS"
                     /locus_tag="VIO83_07380"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY8331132.1"
                     /translation="MDKTKQAVQAAIPRHVAIIMDGNNRWAKKRFLPGVAGHKAGVDA
                     VRAVIEVCAEAGVEVLTLFAFSSENWQRPADEVGALMELFLMALRREAKKLVSNGIRL
                     RVLGDRTRFHPELQAAIGEAEAQAAGGTGMLLQVAANYGGQWDIAQAAQRLAREVAAG
                     HLAADEITPDLLQRCLVTGEQPPVDLCIRTGGEQRISNFLLWQVAYAELYFSDALWPD
                     FKHAAMRAALDDFARRQRRFGKTSEQVAAEQRKPTC"
     gene            20874..21689
                     /locus_tag="VIO83_07385"
     CDS             20874..21689
                     /locus_tag="VIO83_07385"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY8331133.1"
                     /translation="MLKQRIITALIMLPIALGGFFLLQGGWFALFIAAIVCVGAWEWA
                     RLAGLEVQPLRLAYAAGVALLLLGLYQLPQLAGPLLTAAVLWWLAAIGLVLGYPGSSR
                     AWQALPVRLLIGLLILLPAWQGLLLLKQWPQGNSLILAVMVLVWSADIGAYFSGKRFG
                     RRKLALQVSPGKSWEGLYGGLACSLLLTLLVGLYRDWDAAELLVALIGAALVVLISVV
                     GDLTESMFKRQAGIKDSSNLLPGHGGVLDRIDSLTAAVPMFAVLLWLHGWGSL"
     gene            21686..22870
                     /gene="ispC"
                     /locus_tag="VIO83_07390"
     CDS             21686..22870
                     /gene="ispC"
                     /locus_tag="VIO83_07390"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017336704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY8331134.1"
                     /translation="MKQQITVLGATGSIGLSTLDVIARHPHDYAVFALTGYSRLVELE
                     RLCLLHPPRYAVVPSADAARQLQGSLLAAGLHIEVLVGEQGLCEVAAHPEVDAVMAAI
                     VGAAGLKPTLAAVQAGKRVLLANKEALVMSGALFMQAVQTHGAQLLPIDSEHNAIFQC
                     MPGDYARGLGQVGVRRILLTASGGPFRSWPLERLSEATPQQACAHPNWAMGRKISVDS
                     ASMMNKGLELIEACWLFDARPAQVEVVIHPQSVIHSLVDYVDGSVLAQLGNPDMRTPI
                     AHALAWPRRIDSGVAPLDLFAIARLDFEAPDERRFPCLKLARQAAETGGTAPALLNAA
                     NEIAVAAFLDERIRFHEIAHMIDVVLNRIPAVEVRELEQVLAADAQARAVAHEWLAQY
                     GR"
     gene            22887..24239
                     /gene="rseP"
                     /locus_tag="VIO83_07395"
     CDS             22887..24239
                     /gene="rseP"
                     /locus_tag="VIO83_07395"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HEY8331135.1"
                     /translation="MNALYMIVGTLVALGVLVTIHEFGHFWVARRCGVKVLRFSVGFG
                     TPLLRWHDRQGTEYVIAAVPLGGYVKMLDEREGDVPPALLEQAFNRKSVQQRFAIVAA
                     GPVANFLLALLFFWILAMLGSQQVRPVIGSVESGSVAGQAGVVAGAEVLAVDGEPTRG
                     WSEVNLQLIRRLGETGTLRLRLQEPGSTVESERSLALAGWLKNVEDPDPLASLGIRPW
                     RPQIAPVLAQLDPEGPAKAAGLQVGDRLLSVEGRLLDEWQQVVDAVREQPGQRVEFMV
                     ERDGRRFEVSLTLAARGEGKQRSGYLGAGVEGGEWPADMLREVSYGPLDAVGESVKRT
                     WTMSRMTLDSIGKMLFGELSVKNLSGPITIAKVAGASAQSGVGDFLSFLAYLSISLGV
                     LNLLPIPVLDGGHLLFYLVEWARGRPLSERVQAIGMQIGVSLVVGLMLLALFNDISRL
                     "
     gene            24317..26662
                     /gene="bamA"
                     /locus_tag="VIO83_07400"
     CDS             24317..26662
                     /gene="bamA"
                     /locus_tag="VIO83_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEY8331136.1"
                     /translation="MKRLLLPAVIAALMTAEVHAESFTITDIRVNGLQRVSAGSVFGA
                     LPLNVGDAVDDRRLVDATRSLFKTGFFQDIRLGRDGNVLVVDLVERPSISTIEIEGNK
                     AIATDDLLKGLKQSGLAEGEIFQQATLEGVRNELQRQYVAQGRYSATIETKMVSQPRN
                     RVALKIEINEGSVAAIQHINIVGNKVFSSEELAGLFELRTTNWLSFIRSDDKYSREKL
                     SGDLERLRSYYLDRGYINMDISSTQVSITPDKKHVYVTVNINEGEKYRIREVKLAGDL
                     RVPEEEVRQLLLVKEGQIFSRKVMTSTSELITRRLGNEGYTFANVNGIPEAHDEDNTV
                     SLTFAVDPGKRAYVNRINFRGNTKTEDEVLRREMRQMEGGWASTYLIDQSKTRLERLG
                     YFKEVNVETPQVPGTDDQVDVNYSVEEQPSGSVTASVGFAQSAGLILGGSISQSNFLG
                     TGNKVNLGLTRSEYQTRYNFAFTDPYWTEDGVSLGYNMFYRTTDYDDYDVDFTSYSVD
                     SLGAGINLGYPVSETSRLNYGLSVQQDDISPGTYAVDEIRDFVDQEGDSYTNFKGSIG
                     WSESTLNRGMFATRGHSQSLSGEITLPGSDLSFYRMEYNGQVFTPLWGKDYAMRFHTR
                     LGYADSYGSTSELPFYEHYYAGGFGSVRGFEDNSLGPRSTPAAADPDPESDAFGGNVL
                     VQGGAELLFPMPFVKDQRSLRTVLFWDVGNVFDTNCSSAQEARSESGCDLGLSDMASS
                     VGVGLTWLTAMGPLSFSLGMPVMKPDEADTQVFQFSLGQTF"
     gene            26708..27211
                     /locus_tag="VIO83_07405"
     CDS             26708..27211
                     /locus_tag="VIO83_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY8331137.1"
                     /translation="MRKLTQAVLFAAALVSMPAFAEMNIAVLNYQMALLESDAAKKYA
                     VDAEKKFGPQLTRLKTLESDAKRIQDRLMKEGDKLPQSERERLELEFKQKARDFQFQS
                     KELNESKAVADRDMLKQLKPKLDKAVEEVIKSGSYDLVLERGAVVDVKPKFDITPKVI
                     ERMNQQR"
     gene            27211..28272
                     /gene="lpxD"
                     /locus_tag="VIO83_07410"
     CDS             27211..28272
                     /gene="lpxD"
                     /locus_tag="VIO83_07410"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY8331138.1"
                     /translation="MMTVPVFTLGELAQRVGAELRGPADLAISGLAALQDAGPQQLTF
                     LANAQYRKHLASCQAGAVLLTAADAEGYAGAALVLANPYLAFAQLSHLFDRKPVAAPG
                     IHPSAVVAEDACIDPSASIGAGVVVESGARIGARVSLGAYCVVGARSVIGDDGWLAPR
                     VTLYHDVRIGQRVVIQSGAVLGGEGFGFANDKGVWIKIAQIGGVTIGDDVEIGANTTI
                     DRGALADTVIGNGVKLDNQIMIAHNVQIGDHTAMAGCCGISGSTKIGSHCMIAGGVGM
                     VGHIEVCDNVFVTGMTMVTRSITEPGAYSSGTAMQPAGEWKKSAARLRHLDDMAKRLQ
                     QLEKRLAAVTSSGDAASDD"
     gene            28318..28758
                     /gene="fabZ"
                     /locus_tag="VIO83_07415"
     CDS             28318..28758
                     /gene="fabZ"
                     /locus_tag="VIO83_07415"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY8331139.1"
                     /translation="MMDINEIRELLPHRYPFLLVDRVVEMNLEEKRIRAYKNVTINEP
                     FFNGHFPGHPIMPGVLIIEAMAQAAGILGFTMMGAKAAADTLYLFVGSDNLRFRSPVT
                     PGDQLTLEARVLSAKRGLWKFECQASIDGKAVCSGEIICAEQKK"
     gene            28755..29531
                     /gene="lpxA"
                     /locus_tag="VIO83_07420"
     CDS             28755..29531
                     /gene="lpxA"
                     /locus_tag="VIO83_07420"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEY8331140.1"
                     /translation="MSLIDPRAIIDPSAKLADTVEVGPWSIIGPNVEIGEGTVIGPHV
                     IVKGPTVIGRHNRIYQFSSVGEDTPDLKYKGEPTRLVIGDHNVIREGVTIHRGTVQDR
                     YETTIGNHNLLMAYVHIGHDSVIGDHCILVNNTALAGHVHVGDWAILSGFTLVHQFCH
                     IGAHSFSGMGTAIGKDVPAFVTVFGSPAEARSMNFEGMRRRGFSDEVMHALRRAYKVV
                     YRKGMTVEEALAELAADAEQYPEVALFCDSVRNSTRGITR"
     gene            29536..>30501
                     /gene="lpxB"
                     /locus_tag="VIO83_07425"
     CDS             29536..>30501
                     /gene="lpxB"
                     /locus_tag="VIO83_07425"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY8331141.1"
                     /translation="MTRPLKIALVAGEASGDILGAGLMQAIKARHPEVQFIGVGGPRM
                     QAEGLDSYFPMERLAVMGLVEVLGRLPELLKRRRRLIRTLIDERPDVFIGIDAPDFTL
                     GVELKLRQAGIRTVHYVSPSVWAWRQKRVLKIRQACDLMLTLFPFEAQFYEAQQVPVC
                     FVGHPLADTIDLHADRAAARSALDLPGQGGVVALMPGSRGGEVERLGSLFLDTAARLC
                     ALRPGLRFVLPCANAERRAQVEAMLAGRELPVQLLDGRSHEALAACDAVLIASGTATL
                     EALLFKRPMVVAYRVAPLTYRILKRLVKSPYISLPNLLAGRLLVPE"
BASE COUNT         5274 a         9314 c        10453 g         5460 t
ORIGIN      
        1 ccgatgtcga tccagcgcgc gggcgcgcgc ccggcggcca gctcggccag gcgagcggcc
       61 agcggtgcgt agttcccgcc atcgagaaag cgccggcgcg cctcgaccat ggcggcattg
      121 tcgcccgggt cgaggctctt cttgtgctgc accggcagca ggttcaggta gccctggcgg
      181 gcgcggtcga aacgatggcc gtggctgcac tgcacgccgt tgtcgagggt gtgcagcgcg
      241 ccgctgcaga tcgggcagat gagcatgggt cagttcgcca gcagacgaac cagcgtctgg
      301 tagtagattt ccgtaagcag atccaggtcg ctggccagca cgcgctcgtt gacctggtgg
      361 atggtggcgt tgaccgggcc gagttcgacc acctgggtgc ccagcgtggc gatgaagcgt
      421 ccgtcggagg tgccgccgct ggtggaggcg acggtctcgc ggccggtgac gttgcggatg
      481 ctggcggata cggcatccag cagcgcgccc ggctcggtga gaaacggcag gccggacagg
      541 gaccattcga cctgccactt gaggccgtgg cggtcgagga tcgcggcgac gcgctgctgg
      601 agcccttcca cggtggactc ggtggagaag cggaagttga acagcgcgac cagctcgccg
      661 ggaatcacgt tggtggtgcc ggtgcccgag tggatgttgg aaatctggaa gctggtcggc
      721 gggaagaagg cgttgccggc gtcccagtgc tcggcggcta gttcggccag ggctggggcg
      781 accaagtgga tggggttctg cgccaggtgc ggataggcga cgtggccctg tacgccctgg
      841 atgcgcagtt tgccgttgag cgagccgcgg cggccgttct tgaccatgtc gccgaccagc
      901 gaggtgctcg acggctcgcc gacgatgcac cagtccaggc gctcgttgcg cgcggccaga
      961 cgctcgacca ccgcctgggt gccgtgcagg gccgggcctt cctcgtcgct ggtgatcagg
     1021 aaggcgatgg ccccttggtg ctgcggatgg tcggcgacga agcgctccac ggcgacgatc
     1081 atcgccgcca ggctaccctt catgtccgcc gcgccgcggc cgcagagcat gccgtcggca
     1141 tcgacgaagg ccgcgaaggg cggatgctgc caggcttcca gcggcccggt cggcaccacg
     1201 tcggtatgcc cggcgaagca cagcaccggc ccttcgccgc cgcggcgggc ccagaagttt
     1261 tccacgtcct cgatctgcat cggctccaca ttgaagccga ccgcggccag gcgctgcatc
     1321 atcaggctct ggcagccctc gtccagcggg gtgacggagg cgcggcggat cagctcgcag
     1381 gccagttcga gggtggggga ggggggggtc atgcaggctc cggaacgacg acagatcaaa
     1441 ggtgtctatc ttaaagcaaa atcacagctg gtcagagcga agcggtagcc gcatgtattg
     1501 gcgtgcgagc gtgatgccgc cgattagcgg gcgtattggc cgatcagttg cgcagcgttg
     1561 tgagagttgg ttctaacttc atttttgaaa gctgggtagt cttgcactac tcaaccttat
     1621 ttccagccct gtggaggcat tcatggcaaa gaagcaagtt aacaacaagt cgagccagct
     1681 gttggattgg ctggctgcaa atggtgggta caatccggtc gagcttaccg aggatggcgt
     1741 accactggtc ggcaccagtg aagacgatct cctcaccgga ggggctggcg atgacgttat
     1801 cgatggtggc gaaggtgact tcgataccgt caactatggt aacgccaaga aagtgaaggt
     1861 agacctggct gcagagactg ccactggcga aggcaacgat acgctggtca atgtcgagaa
     1921 cgtaatcggt tccgcgaagg gcagcgactt catcacaggt gacgagctgg gcaacatgct
     1981 cgatggctgg ggtggcaacg acaagctgtg gggcgatgcg ggcgacgaca ttctgttcgg
     2041 cggtgacggc aacgaccaac tccgtggtgg cgatggctac gatgagcttg acggtggcgc
     2101 cggcaacgat cagctgttcg gcgatgctgg cgatgacctg ctgtacggtg gcgccggcaa
     2161 tgaccagctg cgcggcggtg agggcaacga tgtcctctac ggcgatgccg gcaacgacac
     2221 gctgcgtggt gaggcgggtg acgacacctt ctatgccggt gctggcaatg acagcatgtt
     2281 cggcggagct ggcaacgaca cctactatgt cgaagatgcc aaggacaagg tcaaggaaaa
     2341 tgctggcgaa ggcaccgatg agatcgtggc cagctactcc tatagcctgg agaaggtaaa
     2401 gaatgtcgag aacctgaccc tttccgaaga cgccaagccg ttgaacatca atgcaaccgg
     2461 caactccttg gacaacgtgc tgactggcaa cagcggtgac aacgtgctga agggcctggc
     2521 tggcaacgac atcctggtcg gtcgcgaagg cgacgatacc ctgatcggtg gcgacggcga
     2581 cgacatcttc gtcttcgaca acctcgatgg cgtcgatagc gttgaggatt tcaccattgg
     2641 tagcgacctg ctgcagctgg acgccctggt gttcacctcc ctggccgcag gcgttcagga
     2701 tgcgaatctg gtgatgggca cagaggccgc cgacagcgac gacttcctgg tcttcgacgc
     2761 ggacaatggc gctctgtact acgacgcaga cgctagtggt gccgaggctg cggtgcagat
     2821 tgccaccgtg ggcgtttcca gtctggtggc tgcggacttc gtggtcgtct gatcctgcag
     2881 gacgctggtt gaaaaaaaag ggggctgccg tatggcagcc ccctttttcg ttgtgcgtcc
     2941 agcctgcgtg caggctggga aatcaatgtc ctttcatgtg ctaaacgatg ctagcgcctt
     3001 aactctcagt aaaggctggg ccccaatccc acgtaagggg gaaggggcga agctcaggct
     3061 caggccggca cctccgcggc atcctcctcc ggcttgggca gcgaggagag cagggccatg
     3121 accagtgcgg ccaggtaggg cagcgactgc accagcagta cgatgaccca gaaggtcacg
     3181 tccagaccgg cgttcttctg ggtcagggaa atgccgacag cggcgcccca cagcagcagc
     3241 atgacgaaga cttcctcgcg cgcctctgca atagctttga gcaggccgtg gttgctgcgc
     3301 atcttcggcg tgcggaagaa cgggatgctg ctggtgacga agccgtacag caccgccttg
     3361 gcgatggtgt gcgacagcgc caggccggct accgcggcgg agatggcgtc gcgcatgttc
     3421 acgccgaccg ctcggcggta gaggaacagg atcttgccca gcttgaacac gaacagggcc
     3481 agcggcggga tggcgaagat catcagcggc gggtcgaccc ggtgcggcac gatgatcatc
     3541 gccgccgacc agagcagggc gccgagggtg aagaagatgt tcatgccatc ggcaatccac
     3601 ggcatccagc cggcgatgaa gtggtagcgc tggccgcggg acagctcgct gcccttgccc
     3661 atgaataggc tggccaggtg gcgcttcatg atctggatgg cgccataggc ccagcggaag
     3721 cgctgcttct tgaagtcgat gaaggtgtcg ggcatcaggc ccttgccgta gctctgctcg
     3781 gagtaggcgg cggacagccc cttctcgaac acgcgcaggc ccagctcggc gtcctcggtg
     3841 atgcaccagt cggcccagcc cagttcgtcg agcaccgagc gacgggtcat ggtcattgtg
     3901 ccgtgctgga tgatcgcgtc gcggtcgttg cgggtcacca tgccgatgtg gaagaagccc
     3961 ttgtattccg catagcagag cttcttgaac agcgattcgt tctggtcgcg gtagtcctgc
     4021 ggcgactgca ccacggcgat ctccgggtcg gcgaagtgcg gcaccatgtg cttgagccag
     4081 ttacgctcga cgcagtagtc ggagtcgatc accgcgatca cttcagcgtc ggcggcggtg
     4141 tgctgcagca cgtagttcag tgcgccgccc ttgaagccgg ccagcggtgc gacgtggaag
     4201 aagcggaagc gcgggccgag ggtggcgcag tgggcctgca ccggctccca gaccgccggg
     4261 tccttggtgt tgttgtcgat caccaggacc tcgaagtccg ggtagtcgag attggccagg
     4321 gcgtcgaggg tctgcttgac catctccggt ggctcgttgt agcagggcac gtggatcgac
     4381 accttgggcc ggtaggcatc gtcgccggtc accggcggga agggccgcac gcgttcgtgc
     4441 ttccacaccg tctcggctag ctcgtgggct tcggtgagca ggacgatcat caccccgact
     4501 gcgccgaggg ccagcagcag gccgacgagg gtgctgaacc agttgctgta ctgctggctg
     4561 tagtcgtagg cgatccacac cagcatggtg gcggcgagga acgagacgat agtcaggaag
     4621 gtgcgaccgc gctggcgcag ggcactgccg tcgatcagca gcagggccag ggcgagcagg
     4681 gcgagcacgc cggaggccac ggccagcagg cgccactgcg ggatttccac cacggttccg
     4741 gtgaggggga acttggcctg gcggtcggcg ttgtagacac cccagtaggc acccaccgag
     4801 ccttcttcgc tggccttcca gggctggtcg aaggcctcga tgacgaaata gttgtagccc
     4861 ttggagttca gcttgttgac cagagtgcgc aggtagatcg cctggtcggc ctggttggcc
     4921 tcggcgccac cacgggtgcg gccgttgctc ggccagccga cttccgccag cagcagcggt
     4981 tttttcggga acagcttctt catgtcgcgg gcgcggtcga gcacgaactg gatcgagtct
     5041 tcgcgcggga cgaactccca gtagggcagt acgtgggcgg cgatcaggtc gacgtgcttg
     5101 gccatttccg gatatttctg ccagatgtgc cactgctcgg cggtggtcac cggtaccttg
     5161 agcgccgaac gcacgcgatc cagatagccg atcatctgtt caatggtgac ttcttcacgg
     5221 aacagggctt cgttgccgac gatgacccgg acgacgctgc gctcgcgttt ggcgatctcg
     5281 ataccgcgct ctatctgcct ttcgttggcg gcaaggtcgt tgctcagcca gatgcccagg
     5341 gtcacgcgca ggccggcttc ctcggccaga cgcgggatgt cggcgagggt cccgtccacc
     5401 gagtaggtac ggatgttgtc ggtgtgggtc gcgaccaggt tgaggtcggc gcggatctcg
     5461 tcttcgctcg gataggtatt gccctgcggg ctctggtgca gacggaacgg cgagaacgag
     5521 aagccggaga tctggtcggg ccagtccggg gctttcagcg gctggttgta ccaggcccac
     5581 aggccggtga acagtgaagc cacggccacg accatgacca ggtggagacc aagtttgcgt
     5641 gcagacattg tatgtatggg ttccagcaga aggaacggga gaaagccgac ggacgccggt
     5701 tggcgcgcat cctactctgc ggcccgacgc cttgtacaga ggtcgcacaa gcgcctccgg
     5761 acccccagtt tgacctgttg ccgggcgatc tgttccaagc cggcacttgg gtgtgacaaa
     5821 cagctcaatt tattaaagac gcgaaagccc cgcacttcca gtgcggggat ggatagcgct
     5881 aacccgcagg gttaatcagg agttttccgt gcacgaatat attcctggac cgcctcaatt
     5941 gatgcccctc ccacactgcc tgcaaagtag ctcggactcc agagtgcatt gcccctatag
     6001 gctggctgat tcagctcagg atacagacgc ttgatgcgcc gactggaggc ccctttcagt
     6061 gagttgacca gcgccgagat gctgaccttc ggtgggtagt tgaccagcag gtgaacatgg
     6121 tccttttcgc cattgaaggt taccagttcc gcctcgaagt cctcgcagac cttggcgaag
     6181 atcgagtgca agctttccag gtgctcggcc gtgaagagcc cctcacggta cttagtgatg
     6241 aagaccaaat ggcagtgcat catggaaacg cagtggcggc ctgttcttgg tgtcttgata
     6301 ttttccatag acctaattat attggcggca tgaaaatgat caaggccctg cgcgtacgca
     6361 ttcgcgacaa gcatgcggcc ctcttgagtg agatggccag gcaggtcaat ttcgtctgga
     6421 attacgtcaa tgagctgagc agtcgctcga tccgagaacg cggccggttt ctcagcgcct
     6481 acgaccttca cccttacctc aaaggctctg gtaagttgct cggactgcac agccagacca
     6541 tccagggcat caccgaagag tacgtcacgc gtcgcaagca gttcaaaaag caacaattgg
     6601 cctggcggcg cagcggcggc agccgccgct cgctcggctg gattccgttc aaagccgcca
     6661 cctggaagga tggtcaggtc gtcttcaacg gccactactt caaggtgtgg gacagctacg
     6721 gcctggccca gtaccgtttc cgctcgggca gcttcaacga agatgcccga ggccgctggt
     6781 acttcaacgt ggttgtcgag gtcgacgtgc agccctcccc gggccaggaa gccctaggca
     6841 ttgacctcgg gctgaaggat gtggccacct gcagcgatgg tacgaagctg gataatgccc
     6901 gtttctaccg cggcctggag ccgaagctgg ctatcgccca gcgggccggg aaaaaggccc
     6961 gcgtgcgggc tatccacgcg aagatcgcca acaggcgcaa ggaagctttg caccagttca
     7021 gccgcgcgct cgtcgagcgc tgcggcacga tcatcgtggg cgatgtcagc tccacgaaac
     7081 tcgtgaaaac ctcgatggcc aagtcggtgc tggacgccgg ctggggtcaa ctgaaagcga
     7141 tgctggccta caagtgcgcg cacgcaggca tcgtgttcaa agaggtcaac gaggcgtaca
     7201 ccacccaaac ctgcagctgt tgcggcgtat tgcctaacag caggccgaga ggtatcgcag
     7261 ggcttggaat aagagaatgg acctgcagtg agtgcggtgt cactcatgac cgcgatatca
     7321 acgcagccag gaatattctc gcggccggac atggccgtcc agcagtggga atccccgtca
     7381 tttagggcgg agaggatgtc aaaatgtagc cgacttgttt acgggggtgg catgcacatc
     7441 gatgagcagc gtttcggcgg tattgcccgc ctgtatggcc gtctcggtct cgagcgtctg
     7501 gcggccgccc atgtggcggt ggtcggcatc ggcggcgtcg gctcctgggc ggccgaggcg
     7561 ctggcgcgca gcggggtggg cgagatctcg ctgttcgatc tcgacgatgt ctgtgtgacc
     7621 aacaccaacc ggcagatcca tgcccacgtc ggcaatatcg gtcgggccaa ggtcgaggcc
     7681 atggccgagc gcatccgggc gatcaatccg gactgtatcg tgcatgcggt gaccgattac
     7741 gtcacccgcg agaccatggc cgattacatt accgagcagc tcgacggggt gatcgactgc
     7801 atcgacagcg tggtggccaa ggcggcgctg atcagctggt gcaagcggcg caagatccag
     7861 attgtcacca ccggcgctgc cggtgggcag ctcgacccga ctcaggtgca agtcgccgat
     7921 ctgaacaaga ccgtcaacga ccccctggtg gccaaggtgc gttcgctgct gcggcgcgac
     7981 tacaacttct cgcgcacccc cgggcgcact tacagcgtgg cctgcgtgta ctcccgcgag
     8041 cagttgcgct atccccagcc agacggcagc gtgtgccagg ccaagggctt tgccggcgag
     8101 ggcgtgcgcc tggactgcgc cggcggtttc ggcgcggtga cgatggtcac cgccaccttc
     8161 ggcatggttg ctgcggcgcg gctggtggac aagctggtgg ctggtgcgcg ccgcccggcg
     8221 gagcgcaaga caatcgtagg gtagaagatt cgcgcagcga tctcctaccg atgctccgcg
     8281 tgctcgatgg tggacaatcg ctggcgcgag ttgtccaccc tacgcctgat ccggctacgc
     8341 cagctcgcgg atccgctgca gcacggcatt cagcccgttg ctgcgcgagg gcgacagctg
     8401 gcgagacagg ccgagctggg tgaaccagtc ggcgacatcc acctcggcca gctcggtcgc
     8461 ggtgaggccg ttgacccgca gcagcagcag ggccagcagg ccgcgcagca ggcgggcatc
     8521 gctgctggcg cgaaagttca agcgcccgtc gtccccgtcg gccaccagcc agacccggct
     8581 ttcgcaaccg tgcacgcggt tgccctcgca ttgctcagct tcactgagcg gtgccaggcg
     8641 ctcgccccac tgcatgagca ggcgcgcgcg ctgctcccag ccggggcagt ggctgaagtc
     8701 ggccagcgcc tcgctggccg cgccaggcag gctcatcgca gcaactccag ggcgtgattg
     8761 agcgcggcga agaagcgctc cagatcgtcg ccttcgctgt acagaccaag cgacacgcgc
     8821 agcgcgccgg gccggccgag gctgtgcagc agcggcatgg cgcagtgctg gccggcacgc
     8881 acggcgatgc cctgctcgct gagcagatgg gacaggtcgg cgttgtgcac gccgtcgacc
     8941 acgaagctgg ccagcgcggt gtgcggctcg ctcaacagct gcaggccgtc gaactggcgc
     9001 aagccggtga gcaggcgctg gtgcagcgcg gcttcgtggg ccgcgacggc gtactcgtcc
     9061 agctcgccca ggtagtcgag ggcggcgccg aggccgatgg cggcggcgat ggccggggtg
     9121 ccggcctcga aacccagcgg cgccggacga aagcgcgcgc tgtggtagtc ggcgtgctgc
     9181 accatctcgc cgccgaactg ccagtggcgc agttgcgcca aggcgcggct gcggccgtag
     9241 agcgcgccga tgccgtccgg ggcgtagagc ttgtggccgg aaaacacgta gaagtcgcag
     9301 tccagtgcct gcaggtcatg ccgaccatgg accgcgccct gggcgccgtc gaccactgtg
     9361 aatgccccct gcgcgcgggc caggggcaac aggcgctcca gcggctgcca gcggcccagt
     9421 acgttggaca gctggctgac cgccaacagg cgggtgcgcg ggccgatcag cagggcggcc
     9481 tgctccaggt cgatatcgcc ggcgccatcc agcggcagca ccaccagctt gagtccgcgg
     9541 cgctgcgcca gttgctgcca aggcagcagg ttggcgtggt gctcgagggc gctgatgacg
     9601 atctcgtcac cggcggagaa caggtgctca aggccgtagg ccagcagatt cagaccctcg
     9661 gtgctgccgc gggtgaagac gatctccgcg gggctgtccg cattcaacca ctgcgccacc
     9721 ttctcgcggg cgccttcgaa ggccagggtg gcccgctcgc ccggaccgtg ctgggcgcga
     9781 tgcacattgg cggcgccgcc ggcgtagtgg tcgagcaggg cgtccagtac cttctgcggc
     9841 ttctgcgcgg tggcggcggt atcgaggtag gtctggcggc tggcggtgaa ggcggcgaag
     9901 gccgggaagt cggcacgcca gggggagggc agggacatgg tggctcctgt cagggccagt
     9961 ggtggactgc cgctatatgg cgagtccacc ctacggtgct ggcgcttaaa aattgctctt
    10021 aaagcgtagc gagcgaaggt caggcaaggc gaagtcaggc gaggacgcgg agtttactga
    10081 gtgtaaatga gcagtactcg tttcactcgc ccttcgggcc gccctgcggg cgttcagtaa
    10141 acagcactgt ccgagcctga cttcaacgca gcatggccga gcgtagtagc tttaagtcaa
    10201 tttttagttg tgcgcgtgca gcacttcgtt cagttcgacc gcggtcttgt tggtcttgca
    10261 ctcgaccgcg ccggtctgcg agttgcggcg cagcagcagg tcgttctgcc cggcgatgtc
    10321 gcgggccttg accacttcca ccagggagcc ctggtcgtcg agcacggcga ccttggtgcc
    10381 ggcagtgatg tacaggccgg attcgacggt gcagcggtcg cccagcggga tgccgatgcc
    10441 ggcattggca ccgatcaggc agccttcgcc gaccgagatg acgatgttgc cgccaccgga
    10501 cagggtgccc atggttgagc agccgccgcc caggtccgag cccttgccga cgaacacgcc
    10561 ggcggagacg cggccttcga tcatgcccgg gccggcggtg ccggcgttaa agttgacgaa
    10621 gccctcgtgc atgatggtgg tgccttcgcc gacgtaggcg cccaggcgca cgcgggcggt
    10681 gtcggcgatg cgcacgccgc tcggcaccac gtagtcggtc atcttcggga acttgtcgac
    10741 cgagaacact tccagcagct cgcccttcag gcgggcttcc agctggcgct cggccagttc
    10801 cgccaggtcg atggcgcctt cgttggtcca cgccacgttg ggcagcagtg cgaagatgcc
    10861 ggccaggctg gtgccgtgcg gcttgaccag gcgatgcgac agcaggtgca gcttgaggta
    10921 ggcctccggg gtgctggccg gtgcgctgtc ttcggccagc agcacggcga ccagcggctt
    10981 gcggctctcg gccaggcggg tgagcagggc ggcctgggcg gcgtcgatct gcttgagggc
    11041 gccggccagg tcatgggcct ggttgttgct cagggcgagg gtctggttgc cgccggtgta
    11101 gccgagggcg ttggcggtgg tagccaccag ttcggccggc gggttgagca gcggcagggc
    11161 gtagaagact tccagccagg tgccctgacg gttctgggtg ccgacgccga aggcgatgct
    11221 gtagaggtgc tgcgacatgg ggattgatcc tttcactatt cagtattcaa tcggcgggct
    11281 caggcgagcg ccgccgcata gagttcgggc ttgaagccga gcagggtccg ctcgccgagg
    11341 tcgaggaccg ggcgcttgat catcgacggc tgggcgagca tcaggtcgat ggcgcgggcc
    11401 tggtcgagat cggccttctg ttcgtcggca agcttgcgga aggtggtgcc ggcgcggttg
    11461 aggaccttgt cccagccatg ctcgttgcac catctttcca ggctggcgcg gtcgatgccg
    11521 gcggccttgt agtcgtgaaa acggtagctc agctgccgtt cgtcgagcca gctgcgcgcc
    11581 ttcttcatgg tatcgcaggc cttgattcca taaagctgca ggtctttgat ttcactgggc
    11641 atttcgtgtt tccgagaatc gtggcggggc gtcggcgggg tcagccgaag gccaggtaca
    11701 gacggtagac cagcgatgac agcatgacca gcacaccgat ggccagtacc gccacgccag
    11761 catcgctcgc gcgctgggtg tagccgacgc cgagcatcag catgccgatg ccgatcagcg
    11821 cggtcatcac gttcagcggg ttgtccatca tccttgacgt tcctgcaggt aggcacggat
    11881 acgctcggcc gcttcgacgc actcggccag cggcgcgacc agggccatgc gcacgcggcc
    11941 ggcgccgggg ttgaggccgt ccactgcgcg cgacaggtag gagccgggca ccacggtgac
    12001 gtgctcgcgc tcgaacagct cgcgggtgaa ggtggtgtcg tcgcccgggg ttttcgccca
    12061 cagatagaag ccgccgtccg ggcgttcgac ctcgagcacg ccgccgagga tgtccagcac
    12121 ggcgtcgaac ttctcgcggt acagggcgcg gttggcctgc acgtgggcct cgtcgttcca
    12181 ggcggcgatg ctcgccagct gggtctgcac cggcatggcg cagccgtggt aggtgcgata
    12241 gagcaggaac ggtttgagga tttcggcgtc gccggcgacg aagccggagc gcaggcccgg
    12301 caggttggag cgcttggaca ggctgtggaa caccacgcag cgcttgaagt cgctgcggcc
    12361 gagttcggcg caggcgctca gcaggccggc gggcgggttc tgctcgtcga agtacagctc
    12421 gctgtagcac tcgtcggcgg cgatcacgaa gtcgtgctgg tcggccaggg cgatcagctg
    12481 cttgagggtg tccagcggca ccagggcgcc ggtggggttg ccgggcgagc agatgaacag
    12541 gatctggcag cgctgccaga cttccgccgg cacggcgtcg aagtcggggt tgaagccgtt
    12601 ctcggccagg cacggcaggt agtgcggctg ggcgccggcg agcagggtgg cgccttcgta
    12661 gatctggtag aacgggttgg ggctgaccac cagggcgtcg gggctgcggt cgaccaccgc
    12721 ctgggtgaag gcgaacagtg cctcgcgggt gccgttgacc ggcagcacgt gcttggccgg
    12781 gtccagccag ccggccggca cgccgaagcg gcgctcacac caggcggcga tggactcgcg
    12841 cagcgccggc aggccgatgg tggagggata cacggcaagc tggtcgaggt tgctggccag
    12901 cgcctcggcg acgaaactcg gcgagcggtg cttgggctcg ccgatggaca gggcgatcgg
    12961 cttcttggtc gccggcggct gggcgccggc aagcagcgcg cgcagcttct cgaacgggta
    13021 gggctgcagg aggtcgaggg cacggttcat caagagttcc tgttcagaag ctgatgcgtg
    13081 acagcgggac ggcgctgtcc tgggtgtcgg aaagctggcg gaccagggat tcctgcagac
    13141 gagcgcagag ttccgggtcg gacagcggct ggtggtgggc gtcggtgacg aagaatacgt
    13201 cttccacgcg ctcgccgagg gtggcgatct tggcgttctg caccgacagg tcgaagtcga
    13261 ggaagatctg cccgacgcgg gccagcaggc cggggcggtc gggggcgatc acctcgatca
    13321 cgctcagcgg gcgctgggcg tcggtatgga tggtcacctg cggggcgaag ccgaagctct
    13381 tcagctggcg cggcacccgg cgcttgatga tggtgctgta gtccagcggg ttcttcagcg
    13441 cgtcgaccag gccctggcgg atctcggcga tgcgctcggg atcgtcgccg atcgagccgc
    13501 cgtcagcgtc cagtaccaca taggtgtcga gggtgaactg gctggtcgag gtgaggatgc
    13561 gcgcgtcctg aatgttcagg ttgagctggt ccatggctgc cacggtcacg gcgaagaaat
    13621 cgtgctggtc cggggcgtag atgaagatct gcgtgccgcc ctcgaattcg cgctgggtgg
    13681 tttccttgat cagcaccagc ggcgcgccgt cgtggggatg gcgtaggatg gcttcggcat
    13741 gccaggcgat atcctcggcg ctgtggcgca ggaagtagtg gtcgcccagt tgcgcccaca
    13801 gttgttcggc ggcatcggcg tcgatgccct cgcgcgcgtg cagcagcttg agcgccgcgt
    13861 gctggttctc gcggatgcgt tcctcgcggt cgatcgggtt ctccaggccg cggcgcaggg
    13921 cgcgcttggt ctcggcgtac aactggcgca tcagtgaggc gcgccaggag ttccacagcg
    13981 tcgggttggt ggcgttgatg tcggccacgg tcagcacgta gaggtagtcg aggtgggtct
    14041 ggtcgccgac cgtgcaggcg aagtcgtgga tcacctgcgg gtcggaaaga tccttgcgct
    14101 gcgcggtggt ggacatgacc aggtggttct tcaccagcca ggcgatcagc cggctgtccc
    14161 acggcggcag gtggtggcgc tcgcagaagg ccagggcatc cttggcgccc agttcggagt
    14221 ggtcgccgcc gcggcccttg gcgatgtcgt ggtacagacc ggcgaagtac accagctcgg
    14281 gcttgggcag ctgggccatc agctcgctgg ccagcgggta cttttccgcc atctccggac
    14341 ggtcgagttt gcgtaggttc ttgatcaggt tgagggtgtg cgcgtcgacc gtatagatgt
    14401 ggaacaggtc gtgctgcatc tgaccgatga tgtggccgaa ctccggcagg tagcggccga
    14461 ggatgccgta gcggttcatc cgccgcaggt tgcggtggat gccctcatgg gacttgaaca
    14521 gctcgacgaa caggctggtg ttgcggatgt catggcggaa cttgtcgtcg atcagctggc
    14581 gatggtcgcg cagcaggcgg atggtgccgg agcgcacgcc gcggatctcc ggctgctggg
    14641 ccatcagcac gaaaatctcc aggatcgcgc tggggcagcg cttgaagacg cccgggtcgg
    14701 tggcctcgat gaagccgtcg cggacctgga agcgcgcatt cagcggccgc agctcgccgg
    14761 agtccggctg ctcgagcagc tgttcctcga gggtctggtt gaccaggtcg ctcagctccg
    14821 cggccgccat caccacccgg tagtacttct gcatgaagct ctcgaccgcc agcttggcgt
    14881 tgctggcggt gaagccgagc aggccggcca ggcgccgctg gtggtcgaac agcaggcggt
    14941 cctcggcgcg cccggcgacc atgtgcaggg cgtagcgcac cttccacagg aactcctggc
    15001 tggcgctcag caggccgaat tcgctcgggg tcagcaggcc gcgggtgacc agggtttcca
    15061 gggtcagggt gccgtagtag cggccgatga cccagaggat gttctgcagg tcgcgcaggc
    15121 cgccgggcga gcccttgacg ttgggctcga ggttgtactc ggtgtcgttg tacttggcgt
    15181 ggcgggcgtg ctgctcggca cgcttggcga gaaagaactc gcgcgccggc cacatgtact
    15241 gggtgccggt ggcggcgagc atgcgtcggc gcaggtcgtc cgcgccgacc agggtgcggc
    15301 tttccatcag gttggtgatc accgtcaggt cggcgcgccc ctgttcggcg cagtcctcca
    15361 ccgaacgcac gctctggccg atctccaggc cgatgtccca cagcagggtg aggaagccct
    15421 cgatcggttc gcgcaggctg tcgtgctgcg cgccgtcgag caggatcagg atgtcgatgt
    15481 ccgagtgcgg atgcagctcg ccgcgaccat agccgccgac cgcgaccagg gcgatgtcgt
    15541 tggcatcgcc ccagtcgaag cgggcccagg cttcgcgcag gatctggtcg acgaaccagg
    15601 cgcggccggt gatcaggcgg cggatgtcgc aggtggcgga gaagcgcccg tcgagtattt
    15661 cgccggcccg gcggatcgcc ttcttgaagg cggcgatcgg gctgctcttc agggcgagct
    15721 cggcctggaa ctggccgcgg tcgaacagct catggtccac ttccggatgc gacatcgggc
    15781 gatctccgtt caggggctgg gcggcgctgc tcagggcagg cagcgggcga tggtgtcgtc
    15841 gttgcgcagg gtgaggatct cgcaaccgtt ttcggtcacc aggagggtgt gttcatactg
    15901 ggcagagagc ttgcggtcct tggtgatcgc cgtccagccg tcgccgagca ggcgggtctc
    15961 gtggcgaccc tggttgatca tcggctcgat ggtgaaggtc atgccggcct tgagttcgag
    16021 gccgctgcca gcgcgcccat agtgcagcac ctgcggctct tcgtggaaca cgttgccgat
    16081 gccgtggccg cagtactcgc gaaccaccga gtaaccgttc gactcggcgt gcttctggat
    16141 gatctcgccg atgtcgccca ggtgggcgcc ggggcgaacc agcgagatgc ccttgtacag
    16201 gcattcctgg gtgatctggc aaagtcgctg ggcccactcg ggcgcgttgc cgaccaggaa
    16261 catcttgctg gtatcgccga agtagccgtc cttgatcacg gtgatgtcga tgttgaggat
    16321 gtcgccatcc ttgagcggct tgtcgttggg gatgccgtgg cagaccacgt ggttgatcga
    16381 ggtgcagatc gacttgggaa acccgtggta gttcagcggc gcgggaacgg cctgctgcac
    16441 gtcgacgatg tagtcgtggc agaggcggtt cagttcctcg gtggtgacgc ccgcgcggac
    16501 gtgcggctcg atcatctcca gcacttcggc ggccaggcgg ccggcgacac gcattttctc
    16561 gatttcctcg ggggtcttga tggtgacggt catcagctct ctctggcgcg ctgcgcctgc
    16621 ctacggtaaa caggaaagga tgtcattcta acagacccag catccgctgc ggacggccgc
    16681 ggcggcgtgc aggcgggttt cgccgagcgt gcatgtatgg tataaagcgc ggcgcttttc
    16741 ggggtagtcc cggaaaggca ccgaaaccac acacgcatcg acacaatcta tcctgggtgc
    16801 ccgcaagggt tggatattgg gatgcgtgga ggctcaaccc gacttatcga ggaatagaaa
    16861 tgtcccaagt caacatgcgc gatatgctca aggccggctg ccacttcggc caccagaccc
    16921 gttactggaa cccgaagatg ggcaagttca tcttcggctc gcgcaacaag atccacatca
    16981 tcaacctcga aaaaaccctg ccgatgttca acgaggccct ggccttcgtt gagaagctgg
    17041 ctgctggcaa gaacaagatc ctgttcgtcg gcaccaagcg ttccgccagc aagatcgtcc
    17101 gtgaagaggc tgcccgcgcc ggcatgccgt tcgtcgatca ccgctggctc ggcggcatgc
    17161 tgaccaactt caagaccatc cgtgcctcga tcaagcgtct gcgcgatctg gaagtgcagt
    17221 cccaggacgg caccttcgag aagctgacca agaaagaagc gctgatgcgc acccgcgatc
    17281 tggaaaagct ggagcgcagc ctgggtggta tcaaggacat gggcggcctg ccggacgccc
    17341 tgttcgtggt cgacgttgat cacgagcgca tcgccatcac cgaagccaac aagctgggta
    17401 tcccggtcat cggcatcgtc gataccaaca gcagcccgga aggtgtggac tacattatcc
    17461 cgggtaacga cgacgccatc cgcgccgttc agctgtacct gggctcgatg gccgacgccg
    17521 tgctgagcgg caagtccaag tccggtgccg agttcgccga agctgcttcg tccgaagctg
    17581 ccgaaggcta agcgccaggc gcgccaagcg ttatccggtg cgcaaaaaag ggggctaggc
    17641 cccctttttg ccacctccga attgccgccc ggcccgccgg gcgaactggt ttagagcaac
    17701 tctagaggac tccgaacatg gcagagatta ctgcagccct ggttaaagaa ctgcgcgagc
    17761 gcactggcca aggcatgatg gattgcaaga aggccctgac tgccgctggt ggcgacatcg
    17821 aaaaggccat cgacgacatg cgtgccgctg gtgccatcaa ggccgccaag aaggccggca
    17881 acatcgctgc cgaaggttcc atcgcggtca aagtggctga cgacaacaag tcggccgtga
    17941 tcctcgaagt caactcccag accgacttcc tggctctgca ggacgacttc aagaacttcg
    18001 ttgctgccag catcgagaag gcctttgccg acaagctggc cgacgtcgct ccgctgatcg
    18061 ctgctcagga gccggctcgc gaagccctgg tttccaagtg cggcgagaac gtcaacatcc
    18121 gtcgcctgac tcgcgtcgaa ggcgacgtgg tgggttccta cctgcacggc caccgcattg
    18181 gtgttgtggt taccctcaag ggtggcaatg ccgagctggc caaggaaatc gccatgcacg
    18241 ttgcggccag caacccgcag ttcgtcagcg ctgccgaggt ttccgcggaa gccgttgcca
    18301 aggaaaagga aatcttcctg gccctgaatg ccgacaagat cgccggcaag ccggaaaaca
    18361 tcgtcgagaa catggtcaag ggtcgcatcg ccaagttcct cgccgaagcc agcctggtcg
    18421 agcagccttt cgtcaagaac cccgatgtca aggtcggcga actggccaag caggccggtg
    18481 ccgaagtcgt ctccttcgtg cgttacgaag tgggcgaggg catcgagaag gctgaagtcg
    18541 acttcgctgc cgaagttgct gcccaggtgg ctgccagcca gcagtaagac gctctgatgc
    18601 tgtccgagaa aaggctgccc gcttccgcgc gcagcctttt ctttgggggc cggcagcgcc
    18661 gatctgccgg gcctgctaca agcaagaaat ggatgctgtt tttcgtgcca tgagggcaca
    18721 gatttcgtcc ggcgtggtca aatccgcgcc ggcatgcgga aggcgccggc ttttcgcaac
    18781 ctttcactgc cgcaggagat tcgtatggcc cagctgagtg gacgccaacc ccgctacaaa
    18841 cgtattttgc tcaagctcag cggtgaagcg ctgatgggca gcgaggagtt cggtatcgat
    18901 cccaaggtgc tcgaccgcat ggcgctggag atcggtcagc tggtgggcat cggcgtccag
    18961 gtcgggctgg tcatcggcgg cggcaacctg ttccggggtg cggcgctgag tgcggccggc
    19021 atggatcggg tcaccggcga tcacatgggc atgctggcca cggtgatgaa ctcgctggcc
    19081 atgcgcgacg ctctggagcg ctcgaacatc ccggctctgg tgatgtcggc gatctccatg
    19141 gtcggggtca ccgaccacta cgaccggcgc aaggccgtgc gccacctggc tagcggggaa
    19201 gtggtgatct tctcggctgg caccggcaat ccgttcttca ccaccgactc ggcggcctgc
    19261 ctgcgtggca tcgaggtcaa tgccgacctg gtgctgaagg cgaccaaggt cgatggggtg
    19321 tacactgccg atccgttcaa ggatcccaat gccgagaagt tcgagcacct cacctatgac
    19381 gaggtgctcg accgcaagct gggcgtgatg gatctgacag ccatctgcct gtgccgcgat
    19441 cacaagatgc cgctgcgggt gttcaacatg aacaagcccg gagccttgct taatattgtt
    19501 gtcgggggcg ccgaaggcac cctggtcgag gaggcttgag aatgatcaac gagatcaaga
    19561 aagacgcgca ggagcgcatg aggaagtcgc tggagtcgct ggatcacgcc tttgccaaga
    19621 tccgcaccgg ccgcgcccac ccgagcattc tggacagcgt catggtgtcc tactacggct
    19681 ccgatacccc gctgcgccag gtggccaacg tgatcgccga ggattcgcgc acgctggccc
    19741 tgaccgtgtt cgacaagagc atgatccagg cggtcgagaa ggcgatcatg acctccgatc
    19801 tggggctcaa tccggccact gccggcacca ccatccgcgt gccgatgccg gccctgaccg
    19861 aggaaacccg caagggcttc accaagcagg cgcgtgccga gtcggagaat gcccgcgtgg
    19921 cgatccgcaa tatccgccgc gatgccctgg cccagctcaa ggatctggtc aaggaaaagg
    19981 aaatcagcga ggacgacgag cgtcgcgccg ccgacgaggt gcagaagctg accgacaagg
    20041 ccgtggccga tgtcgagaag gcgctcgaag ccaaggaagc cgacctgatg gccgtctgac
    20101 ggccggatga cagtcatgga caagacgaaa caagccgtgc aggccgccat tccgcgccac
    20161 gttgccatca tcatggatgg caacaaccgc tgggcgaaga aacgcttcct gcccggcgtg
    20221 gccgggcaca aggcgggcgt cgacgcggtg cgtgcggtga tcgaggtgtg tgccgaggcg
    20281 ggcgtcgagg tgctgactct gttcgccttc tccagcgaga actggcagcg tccggccgac
    20341 gaggtgggtg cgctgatgga gttgttcctc atggccctgc gccgcgaggc caagaagctg
    20401 gtgagcaacg gcatccgcct gcgggtgctg ggtgatcgca cgcgcttcca tccggaactg
    20461 caggccgcga ttggcgaagc cgaggcgcag gccgctggcg gcacggggat gctgctgcag
    20521 gtggcagcca actacggcgg ccagtgggac atcgcccagg ccgcgcagcg tctggcgcgc
    20581 gaggtggcgg ctggtcatct ggcggccgac gagatcacgc cggacctgct gcagcggtgc
    20641 ctggtcaccg gcgagcagcc gcctgtcgat ctgtgcatcc gtaccggcgg cgagcagcgc
    20701 atcagcaatt tcctgctctg gcaggtggcc tacgccgagc tgtatttctc cgacgcgctc
    20761 tggccggact tcaagcacgc agccatgcgt gccgcactgg acgattttgc ccgccggcag
    20821 cgccgcttcg gcaagaccag cgagcaggtg gcggcggaac aacggaaacc tacatgctga
    20881 aacagcgcat catcactgcc ctgataatgc tgcccatcgc tctgggcggc ttttttctcc
    20941 ttcagggtgg ctggtttgcc ctgttcattg ccgcgatcgt ctgtgtcggt gcgtgggaat
    21001 gggcgcgcct ggccgggctc gaggtgcagc cgctgcgcct ggcctatgcg gccggcgtcg
    21061 cgctgttgct gctcgggttg taccagctgc cgcagctggc ggggccgctg ctgacggcgg
    21121 cggtgctctg gtggctggcg gcgatcggcc tggtgctggg ttatcccggc agcagtcgtg
    21181 cctggcaggc gctgccggtg cgtctgctga tcggcctgct gatcctgctg ccggcctggc
    21241 agggcctgct gctgctcaag cagtggccgc agggcaacag cctgatcctg gcggtgatgg
    21301 tgctggtgtg gagcgccgat atcggcgcct acttcagcgg caaacgcttt ggtcggcgca
    21361 agctggcgct gcaggtcagt cccggcaaga gctgggaggg cctctacggg gggctggcct
    21421 gcagcctgct gctcaccctg ctggtcggcc tgtatcgcga ctgggatgcc gcggagctgc
    21481 tggtcgcgct gatcggcgcg gcgctggtgg tgctgatctc ggtggtcggc gatctcaccg
    21541 aaagcatgtt caagcgccag gctgggatca aggacagcag caacctgctg ccgggtcacg
    21601 gtggcgtgct ggatcgcatc gacagcctga cggcggcggt gccgatgttt gccgtgctgc
    21661 tctggctgca tggctggggt agcctgtgaa gcaacagata accgtactgg gtgcgaccgg
    21721 ttccatcggt ctgtccaccc tcgatgtaat cgcccgccat ccgcacgact acgcggtgtt
    21781 cgccctgacg ggctacagcc ggctggtcga gctggagcgc ctgtgcctgc tgcatccacc
    21841 gcgttatgcc gtggtgccga gtgccgacgc cgcgcggcaa ctgcagggca gtctgctggc
    21901 tgccggcctg cacatcgaag tgctggtcgg cgagcagggc ttgtgcgaag tggcggcgca
    21961 tcccgaggtg gatgcggtga tggcggccat cgtcggcgct gccggcctca agccgacctt
    22021 ggcggcggtg caggcgggca agcgcgtgct gctggccaac aaggaagccc tggtgatgtc
    22081 cggggccctg ttcatgcagg ccgtgcagac ccacggggcg cagctgctgc cgatcgacag
    22141 cgagcacaac gccatcttcc agtgcatgcc gggcgactat gcgcgcggcc tggggcaggt
    22201 cggcgtgcgc cggatcctgc tcaccgcctc aggcgggccg ttccgcagct ggccgctgga
    22261 gcgcctgagc gaggccacgc cgcagcaggc ctgtgcccat cccaactggg cgatggggcg
    22321 caagatctcg gtcgactcgg cgagcatgat gaacaagggc ctggagctca tcgaggcctg
    22381 ctggctgttc gatgcgcgac cggcccaggt cgaggtggtg atccatccgc agagcgtgat
    22441 tcattcgctg gtcgactacg tcgacggctc ggtactggcc cagctcggca atccggacat
    22501 gcgcacgccg atcgcccatg ccctggcgtg gccgcggcgc atcgattcgg gtgtcgcgcc
    22561 gctggatctg ttcgccatcg ctcgcctgga tttcgaagcg ccggacgagc ggcgtttccc
    22621 ctgcctgaag ctggcgcggc aggccgccga aaccggcggc acggcgccgg cgctgctgaa
    22681 tgccgccaac gagattgcgg tggcggcttt tcttgacgag cgcatccgct tccacgagat
    22741 tgcacacatg atcgacgtgg tgttgaaccg cattcctgcc gtcgaggtcc gcgagctgga
    22801 gcaggtgctg gccgccgacg cgcaggcgag agcggttgcc cacgaatggc tggcgcaata
    22861 cggacgttga gtccggaggt gttccgatga atgcgctgta catgattgtg ggcaccctgg
    22921 tggcattggg cgtgctggtt accatccacg agttcggcca cttctgggtg gcgcggcgct
    22981 gtggcgtcaa ggtcctgcgc ttctccgtgg ggttcggcac gccactgctg cgctggcacg
    23041 accgtcaagg taccgagtac gtcatcgcgg cggtgccgct gggcggctac gtgaagatgc
    23101 tcgacgagcg cgagggcgat gtaccgccgg ccctgctgga gcaggcgttc aaccgcaaga
    23161 gcgtgcagca gcgctttgcc atcgtggctg ccggcccggt ggccaacttc ctgctggcgc
    23221 tgctgttctt ctggattctg gccatgctcg gcagccagca agtgcgcccg gtgatcggca
    23281 gcgtcgagtc gggcagtgtg gccgggcagg cgggggtggt ggccggtgcc gaagtactgg
    23341 cggtggacgg cgagccgacg cgcggctgga gcgaggtcaa tctgcagctg attcgccgtc
    23401 tcggcgaaac cggtaccctg cgcctgcgcc tgcaggagcc ggggtcgacg gtggaaagcg
    23461 agcgcagcct ggcgctcgcc ggctggctca agaatgtcga ggatcccgat ccgctggcgt
    23521 cgctggggat ccgcccgtgg cggccgcaga ttgcgccggt gctggcccag ctcgatcccg
    23581 aggggccggc caaggcagcc ggcctgcagg tgggggatcg actgctgagc gtggaagggc
    23641 gcctgctgga cgagtggcag caggtggtcg atgccgtacg cgagcagccg ggtcagcgcg
    23701 tcgagttcat ggtcgagcgt gacggtcgcc gtttcgaggt gagcctcacc ctggctgcgc
    23761 gtggcgaagg caagcagcgc agcggctatc tcggtgccgg tgtcgagggt ggcgaatggc
    23821 cggcggacat gctgcgcgag gtcagttacg gtccgctgga tgccgtgggg gagagcgtca
    23881 agcgcacctg gacgatgagc cggatgaccc tcgactcgat cggcaagatg ctgttcggcg
    23941 agctctcggt aaaaaacttg agtggcccga taacgattgc taaagtggcc ggcgcttcgg
    24001 cccagtctgg ggtgggggat ttcctgagtt tccttgccta cctgagcatt agcctggggg
    24061 tgctcaacct gttgccgatt ccggtcctcg atggcgggca tctgctgttc tatctggtcg
    24121 agtgggcccg tggccggcca ctctccgagc gggtacaggc gattggcatg cagataggcg
    24181 tcagtctggt tgtcggcctg atgctcctcg cgctgtttaa tgacatcagc cgactgtagc
    24241 ctgccatgca ttgcggagct gccgcctgtt gcggcagttc ctttttagtc agtctgaaaa
    24301 gtatagaaag gacttcatga aacgcttgct gctaccggcg gtaattgccg cgctgatgac
    24361 cgccgaggtt cacgccgagt cctttaccat caccgatatt cgcgtcaacg gcctgcagcg
    24421 ggtgtctgcc ggcagcgtgt tcggtgcctt gccgctcaat gtgggtgacg cggtcgatga
    24481 ccgccggctg gtggatgcga cccgctccct gttcaagacc ggtttcttcc aggatattcg
    24541 cctcggtcgc gacggcaacg tgctggtggt cgatctggtc gagcgtccgt cgatctccac
    24601 tatcgagatc gagggcaaca aggccatcgc caccgatgat ctgctcaagg gcctgaaaca
    24661 gtccggcctg gccgagggcg aaatcttcca gcaggccacc ctcgagggcg tgcgcaacga
    24721 gctgcagcgc cagtacgtgg cccagggccg ctattcggcc accatcgaga ccaagatggt
    24781 cagccagccg cgtaaccgcg tggcgctgaa gatcgaaatc aacgaaggct cggtcgcggc
    24841 gatccagcac atcaacatcg tcggcaacaa ggtgttttcc agcgaagagc tcgccggcct
    24901 gttcgagctg cgcaccacca actggctgtc gttcatccgc agcgatgaca agtactcccg
    24961 cgaaaagctg tccggcgacc tcgagcgcct gcgttcgtat tacctcgatc gcggctacat
    25021 caacatggac atctcctcga cccaggtgtc catcacgccg gacaagaagc acgtctacgt
    25081 caccgtcaac atcaacgagg gcgagaagta ccgcatccgc gaggtgaagc tggccggtga
    25141 tctgcgcgta cccgaggaag aggtgcgcca gctgctgctg gtcaaggaag ggcagatctt
    25201 ctcgcgcaag gtgatgacca gtacttccga gctgatcacc cggcgcctgg gtaacgaggg
    25261 ctacaccttt gccaacgtca acggcattcc cgaggcacat gacgaggaca ataccgtcag
    25321 cctgaccttc gccgtcgatc cgggcaagcg tgcctacgtc aaccgcatca acttccgcgg
    25381 caacaccaag accgaggacg aggtgctgcg tcgcgagatg cgccagatgg agggcggctg
    25441 ggcctcgacc tacctgatcg accagtccaa gacccgtctc gagcgcctcg gctacttcaa
    25501 ggaagtcaac gtcgaaaccc cgcaggtgcc tggcaccgac gaccaggtcg acgtcaacta
    25561 cagcgtcgag gagcagccgt ccggctcggt caccgccagc gtcggctttg cgcagagcgc
    25621 cggcctgatc ctcggcggct cgatcagcca gagcaacttc ctcggcaccg gcaacaaggt
    25681 caacctcggc ctgacccgca gcgagtacca gacccgctac aacttcgcct tcaccgatcc
    25741 ctactggacc gaagacggcg tcagcctcgg ctacaacatg ttctaccgca ccaccgacta
    25801 cgacgactac gacgtcgact tcaccagcta ttcggtggac agtctgggcg cggggatcaa
    25861 cctcggctac ccggtcagcg aaacctcgcg gctgaactat ggcctgtcgg tccagcagga
    25921 cgatatcagc cccggtacct acgcggtcga cgagatccgt gacttcgtcg accaggaagg
    25981 cgacagctac accaacttca agggctcgat cggctggtcg gagtcgaccc tcaaccgcgg
    26041 catgttcgcc acccgcggtc attcgcagag cctgagcggc gagatcaccc tgccgggcag
    26101 cgatctgtcc ttctatcgta tggaatacaa cgggcaggtg ttcacgccgc tgtggggtaa
    26161 ggactacgcc atgcgcttcc acacccgcct gggttacgcc gacagctacg gctcgaccag
    26221 cgagctgccg ttctacgagc actactacgc cggtggcttc ggctcggtgc gcggcttcga
    26281 ggacaacagc ctcggcccgc gcagcacgcc ggcagcggcg gacccggatc cggaaagcga
    26341 cgccttcggt ggtaacgtgt tggtgcaggg cggcgcggag ctgctgttcc ccatgccgtt
    26401 cgtcaaggat cagcgctcgc tgcgcacggt gctgttctgg gacgtgggta acgtgttcga
    26461 caccaattgc agcagtgctc aggaggcgcg cagcgagtcc ggatgtgacc tcggtctgag
    26521 tgatatggcc agctcggtgg gtgtcggcct gacctggctg acggccatgg gcccgctgag
    26581 tttcagtctc ggcatgccgg tcatgaagcc cgacgaagcc gatacccagg tgttccagtt
    26641 ctccctggga cagactttct aagcccttcg gggccactaa ccaaccgatt tctagcagga
    26701 gttcatcgtg cgcaagttga cccaagctgt cctgttcgcc gctgctctgg tgagcatgcc
    26761 ggccttcgct gaaatgaata tcgcagttct caattaccag atggccctgc tcgaatccga
    26821 tgccgccaag aagtatgcgg tggatgcgga gaagaagttt ggcccgcagc tcacccgtct
    26881 gaagaccctg gagagcgatg ccaagcgcat tcaggaccgt ctgatgaagg aaggcgacaa
    26941 gctgccgcag agcgagcgcg agcgtctcga gctggaattc aagcaaaagg cccgcgattt
    27001 ccagttccag tccaaggagc tgaacgaatc caaggccgtt gccgaccgcg acatgctcaa
    27061 gcagctcaag ccgaagttgg acaaggctgt tgaggaagtg atcaagagcg gcagctacga
    27121 tctggtgctc gagcgcgggg cggtggtcga cgtcaagcct aagttcgaca tcacccccaa
    27181 ggtgatcgag cgcatgaacc agcagcgctg atcatgacgg ttcccgtttt cactctgggc
    27241 gagttggctc agcgtgtcgg tgctgagctg cgtggtccgg cggacctggc catcagtggt
    27301 ctggccgccc tgcaagatgc gggcccgcag cagctgacct ttctcgccaa cgcacagtac
    27361 cgcaagcacc tggccagctg ccaggccggg gcggtgctgc tgaccgcggc cgatgccgaa
    27421 ggttatgccg gcgctgcgct ggttctggcc aatccctatc tggcctttgc ccagttgtcg
    27481 catctgttcg accgcaagcc ggttgctgcg cccggcatcc atccgagtgc tgtagtcgcc
    27541 gaggatgcct gcatcgatcc gagcgcttcg atcggtgccg gtgtggtggt cgagtctggc
    27601 gcgcgcatcg gtgcgcgggt gagtctcggt gcctactgcg tggtcggcgc gcgcagcgtg
    27661 atcggcgacg acggctggct ggcaccgcgc gtgaccctct atcacgacgt acgcattggc
    27721 cagcgcgtgg tcatccagtc cggggccgtg ctgggtggcg aaggtttcgg ttttgccaac
    27781 gacaagggcg tgtggatcaa gattgcccag atcggcggcg tgaccatcgg cgacgatgtc
    27841 gagatcggcg cgaacaccac catcgaccgc ggcgcactcg ccgatacggt gatcggcaac
    27901 ggcgtcaagc tggataacca gatcatgatc gcgcacaacg tgcagatcgg cgaccacacc
    27961 gccatggccg gctgctgcgg catttccggc agcaccaaga tcggcagtca ctgcatgatc
    28021 gccggcggtg tcggcatggt tggccatatc gaggtgtgcg acaacgtatt cgttaccggc
    28081 atgaccatgg tcacccgctc gatcaccgag ccgggtgcct attcctcggg caccgccatg
    28141 cagccggccg gcgagtggaa gaagagcgca gcgcgccttc gtcatctgga cgacatggcc
    28201 aagcgcctgc agcagctgga aaaacgcctg gctgcggtga cctccagcgg tgatgcggca
    28261 tcagatgact gatgcgtgct tccccgcgtt ccctattttg atcaaggccc ccacgacatg
    28321 atggatatca acgagattcg cgaactccta ccgcaccgct acccgttcct gctggtggat
    28381 cgggtggtgg agatgaatct cgaggaaaag cgcatccgcg cctacaagaa cgtgaccatc
    28441 aacgaaccgt tcttcaacgg ccactttcct ggccatccga tcatgccggg cgtgctgatc
    28501 atcgaggcca tggcccaggc ggccggcatt ctcgggttca ccatgatggg ggccaaggcc
    28561 gccgccgata ccctgtacct gttcgtcggc tccgacaacc tgcgcttccg ctcgccggtg
    28621 accccggggg atcagctgac cctcgaggcc cgcgtcctca gtgccaagcg cggcctctgg
    28681 aagttcgagt gccaggccag catcgacggc aaggcagtct gctcgggtga gatcatctgc
    28741 gcggaacaga aaaaatgagt ttgatcgacc ctcgcgccat catcgatccg agtgccaagc
    28801 tggccgacac tgtcgaagtc ggcccctggt cgatcatcgg gccgaacgtg gaaattggcg
    28861 agggtaccgt gatcggcccg cacgtgatcg tgaagggccc cacggtgatc ggccggcaca
    28921 accgcatcta ccagttttcc tcggtaggcg aagacacccc cgatctgaag tacaagggcg
    28981 agccgacccg gctggtgatt ggcgatcaca acgtgatccg cgagggcgtc accatccatc
    29041 gcggtaccgt gcaggatcgc tacgagacca ccatcggcaa ccacaacctg ctgatggcct
    29101 acgtgcacat cggtcacgac agtgtgatcg gcgaccactg catcctggtc aacaacaccg
    29161 cgctggccgg gcatgttcac gttggcgact gggcgatcct ctccgggttc accctggttc
    29221 accagttctg ccatatcggc gcgcacagct tctccggcat gggcacggcg atcggcaagg
    29281 acgtaccggc cttcgtcacc gtattcggca gtccggccga ggcgcgcagc atgaacttcg
    29341 agggcatgcg ccggcgtggc ttcagcgacg aggtgatgca cgccctgcgc cgtgcctaca
    29401 aggtcgtgta ccgcaagggc atgaccgtag aggaggcgct ggccgagctg gccgcggatg
    29461 ccgagcagta cccggaagtg gcgctgttct gcgactcggt gcgcaactcg actcgcggca
    29521 tcacccgctg atccgatgac tcgtccattg aagatcgccc tggtcgccgg cgaggcgtcc
    29581 ggcgacatcc tcggcgccgg cctgatgcag gccatcaagg ctcgccatcc cgaggtgcag
    29641 ttcatcggtg tcggcggccc gcgcatgcag gccgagggtc tcgactccta ctttcccatg
    29701 gagcgcctgg cggtcatggg cctggtcgag gtgcttggcc gcctgcccga gctgctcaag
    29761 cgccgccgtc gcctgatccg caccctgatc gacgaacgcc cggacgtgtt catcggcatc
    29821 gacgcccccg acttcaccct cggggtcgaa ctcaagctgc gccaggccgg catccgcacc
    29881 gtgcactacg tcagcccgtc ggtgtgggcc tggcggcaga agcgcgtgct gaagatccgt
    29941 caggcctgcg acctgatgct gaccctgttc cccttcgagg cgcagttcta cgaagcgcag
    30001 caggtgccgg tgtgcttcgt cggccatccg ctggccgata ccatcgatct gcatgccgac
    30061 cgcgctgccg cgcggagcgc gctcgacctg ccggggcagg gcggcgtcgt ggcgctgatg
    30121 cccggcagtc gcggtggcga ggtcgagcgc ctgggcagtc tgttcctcga cacggcggcg
    30181 cgactgtgtg cgttgcgccc cggtctgcgt ttcgttctgc cctgtgccaa tgccgagcgc
    30241 agggctcagg tcgaggccat gctcgccggt cgcgagctgc cggtgcagct gctcgatggc
    30301 cgctcccacg aggcgctggc cgcctgcgat gcggtactga tcgcctccgg cacggcgact
    30361 ctcgaggcgc tgctgttcaa gcggccgatg gtggtggcct accgcgtggc gccgctgacc
    30421 taccgcatcc tcaagcgcct ggtgaaaagc ccgtacatct cgctgcccaa cctgctggcc
    30481 gggcgcctgc tggtgcccga g
//
LOCUS       DATCUN010000185         3958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_120635, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000185 DATCUN010000000
VERSION     DATCUN010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120635"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VIO83_07430"
     CDS             <1..209
                     /locus_tag="VIO83_07430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEY8331142.1"
                     /translation="ARGIGLGAALFGLAHAPFSLPFALVAGVAGLGYGWVLQLSGRLY
                     AALLLHGAVNLLHFLLLSYPLRLG"
     gene            complement(301..612)
                     /gene="sutA"
                     /locus_tag="VIO83_07435"
     CDS             complement(301..612)
                     /gene="sutA"
                     /locus_tag="VIO83_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SutA"
                     /protein_id="HEY8331143.1"
                     /translation="MSDEDLEQDELEGAEEAEEELGAAPDDDGDADDEVVAPVVKKGK
                     KEAEPEELPSIEAKQKERDALARAMEEFLARGGKVQEVESNVVADPPKKPDSKYGSRP
                     I"
     gene            complement(841..2094)
                     /locus_tag="VIO83_07440"
     CDS             complement(841..2094)
                     /locus_tag="VIO83_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY8331144.1"
                     /translation="MDSNALISLHYALDTFYLLICGALVMWMAAGFAMLESGLVRSKN
                     TTEILTKNVILYGVACVMYLLVGYHIMYSSPEGGILPSLGFLIGAEHAADLVAAGGDD
                     APAFSKRADFFFQVVFVATAMSIVSGAVAERMKLWAFLAFAVVMTGFIYPVQGYWKWG
                     SGFLNQAGFLDFAGSGVVHLCGATAALAGVLMLGARRGKYASNGQVNAIPGANLPLAT
                     LGALILWFGWFGFNGGSQLKISSIGDANAVASAFLNTNMAAAGGLLAALVVARLLFGK
                     ADLTMALNGALAGLVAITAEPVTPAALTAVLIGAVGGVLVVGGVLLLDKLKIDDPVGA
                     IAVHGLGGIWGLLAVPLTNDGGSFTAQLLGIVCIFTWVFVASLIVWAVLKAVMGLRVD
                     AEDEYAGVDIAECGMEAYPEFTRKS"
     gene            complement(2169..3488)
                     /locus_tag="VIO83_07445"
     CDS             complement(2169..3488)
                     /locus_tag="VIO83_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY8331145.1"
                     /translation="MTLRKIAGLGALLSLASPGLALAAEEVTLNSGDTAWMLTATVLV
                     LLMTIPGLALFYGGMVRAKNLLSVMMQCFAITGLISVLWVVYGYSLAFDTTGMEQGVV
                     NFNSFVGGLSKAFLSGLTPSSLIGAFPESVFITFQMTFAIITPALIVGAFAERMKFSA
                     MLIFMAVWFTLVYAPIAHMVWAGNGGLMWDWGVLDFAGGTVVHINAGIAGLVACIVLG
                     KRKGYPTTHMAPHNLGYTLIGAAMLWVGWFGFNAGSAIAANGTAGMAMLVTQIATAAA
                     ALAWMFAEWVTHGKPSALGIASGVVAGLVAITPAAGTAGPMGALVIGLASGLICFFCA
                     TSLKRKLGYDDSLDAFGVHGVGGIVGALLTGVFAAPALGGFGTVEDIGAQLFTQFKGV
                     AFTVVYTAIVTFVILKVLDLVMGLRVSEETESVGLDLAEHNERGYNL"
     gene            complement(3549..3887)
                     /gene="glnK"
                     /locus_tag="VIO83_07450"
     CDS             complement(3549..3887)
                     /gene="glnK"
                     /locus_tag="VIO83_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY8331146.1"
                     /translation="MKLVTAIIKPFKLDDVRESLSEIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVAIADDQLDSVIDAITKAANTGKIGDGKIFVVSLEQAIRI
                     RTGETGTDAI"
BASE COUNT          698 a         1304 c         1320 g          636 t
ORIGIN      
        1 ccgcccgcgg catcggcctg ggcgccgcgc tgttcggcct ggcccatgcg ccgttcagcc
       61 tgccgttcgc cctggtcgcc ggcgtggccg ggctgggcta tggctgggtc ctgcagctgt
      121 ccggccggct gtacgccgcg ctgctgctgc acggggcggt caacctgctg cacttcctgc
      181 tgctgagcta cccgctgcgc ctgggctgaa cggccatccg ccgccgcagg ctgcgcgacg
      241 cagaaatgaa aagggcgacc ccgaggggtc gcccttgcgc cagccagtga tcggaagcga
      301 tcagatgggg cggctgccgt atttgctgtc cggctttttg ggcggatcgg caaccacgtt
      361 ggattcgact tcctgcacct tgccgccacg ggccaggaac tcctccatcg cccgggccag
      421 cgcatcgcgc tctttctgct tggcctcgat cgatggcagc tcttccggct cggcttcctt
      481 cttgcccttc ttgactaccg gagcgaccac ctcgtcgtct gcatcgccgt catcatccgg
      541 cgcggcaccc agctcctctt cggcctcctc ggcaccttcc agctcgtctt gttccagatc
      601 ttcgtcgctc atgatgaaac cccatggtgc agcaaaacag gttggttatg gcccggcagc
      661 gtcgcgtgtc tgcgccgccg gtgcaagcat tgatggactc gccacggatg ccgccgagcg
      721 gcacgagcag gcaaggctga cagcccattc gcgcgcattc tagcgcggcg ccagaaaaag
      781 caaagggcgc ctaagggcgc cccatgcatt caagcagata ctttacgcaa cgccagccgc
      841 tcagctcttg cgggtgaact ccggataggc ctccatgccg cactcggcga tgtccacgcc
      901 ggcgtactcg tcctcggcat ccacccgcag gcccatgacc gccttgagga cggcccagac
      961 gatcaggctg gcgacgaaca cccaggtgaa gatgcagacg atgccgagca gctgggcggt
     1021 gaagctgccg ccgtcattgg tcagcggcac ggcgagcagg ccccagatgc cgcccaggcc
     1081 atgcaccgcg atggcgccca ccgggtcgtc gatcttcagc ttgtccagca gcagcacgcc
     1141 acccaccacc agcacgccgc ccacggcgcc gatcagcact gcggtcagcg ccgccggggt
     1201 caccggctcc gcggtgatcg ccaccagtcc ggccagggcg ccgttgaggg ccatggtcag
     1261 gtcggccttg ccgaacagca ggcgcgcgac gaccagcgcc gccagcagac cgccggccgc
     1321 cgccatgttg gtgttgagga aggccgaggc cacggcgttg gcgtccccga tgctgctgat
     1381 cttcagctgc gagccgccgt tgaagccgaa ccagccgaac cacaggatca gcgcaccgag
     1441 ggtggccagc ggcaggttgg cgccgggaat ggcgttgacc tggccgttgc tggcgtactt
     1501 gccgcgccgc gcgccgagca tcagcacgcc ggccagcgcc gcggtggcgc cgcacaggtg
     1561 caccacaccg gaaccggcga agtcgaggaa accggcctgg ttgaggaaac ccgaacccca
     1621 tttccagtag ccctgcaccg ggtagatgaa gccggtcatc accaccgcga aggcgaggaa
     1681 ggcccacagc ttcatgcgct cggccaccgc gccggaaacg atcgacatgg cggtagcgac
     1741 gaacaccacc tggaagaaga agtcggcacg tttggagaag gccggcgcgt cgtcgccacc
     1801 ggcggcgacc aggtccgccg cgtgctcggc gccgatcaga aagcccaggc tcggcaggat
     1861 gccgccctcc gggctggagt acatgatgtg gtagccgacc agcaggtaca tcacgcaggc
     1921 cacgccgtag aggatgacgt tcttggtgag gatctcggtg gtgttcttcg agcgcaccag
     1981 accggactcg agcatggcga agccggccgc catccacatc accagggcgc cgcagatcag
     2041 caggtagaag gtgtcgaggg cgtagtgcag ggagatcaga gcattgctgt ccatggggaa
     2101 attccttgtg ccaggggcgc gttgcacgcc tcggtcgcgg gcgcccgcac ggggcgcccg
     2161 ccacgtactt acaggttgta gccgcgctcg ttgtgctcgg cgaggtcgag gccgacgctc
     2221 tcggtttcct cgctgacgcg caggcccatc accagatcga gcaccttgag gatcacgaag
     2281 gtgacgatgg cggtgtagac cacggtgaag gccacgccct tgaactgggt gaacagctgg
     2341 gcgccgatgt cttccacggt gccgaagcca cccagggccg gtgcggcgaa cacgccggtc
     2401 agcagggcgc cgacgatgcc gccgacgccg tgcacgccga aggcgtccag cgagtcgtca
     2461 tagcccagct tgcgcttgag gctggtggcg cagaagaagc agatcagacc cgaggccagg
     2521 ccgatcacca gggcgcccat cgggccggcg gtaccggcgg ccggggtgat ggcgaccagg
     2581 ccggcgacca cgccggaggc gatgcccagg gcgctgggtt tgccgtgggt gacccactcg
     2641 gcgaacatcc aggccagggc ggcggcggcg gtggcgatct gggtgaccag catggccatg
     2701 ccggcggtgc cgttggcggc gatcgccgag ccggcgttga agccgaacca gccgacccac
     2761 agcatggcgg cgccgatcag ggtgtagccg aggttgtgcg gcgccatatg ggtggtcggg
     2821 tagcccttgc gcttgcccag cacgatgcac gccaccagac cggcgatacc ggcgttgatg
     2881 tgcaccacgg tgccgccggc gaagtccagc acgccccagt cccacatcag gccgccgttg
     2941 ccggcccaga ccatgtgcgc gatcggcgcg tagaccagag tgaaccacac ggccatgaag
     3001 atcagcatcg cggagaactt catgcgctcg gcgaaggcac cgacgatcag cgccggggtg
     3061 atgatggcga aggtcatctg gaaggtgatg aacacgcttt ccgggaaggc gccgatcagg
     3121 ctgctggggg tcaggccgct gaggaaggcc ttggacaggc cgccgacgaa ggaattgaag
     3181 ttgacgaccc cctgctccat gccggtggtg tcgaaggcca ggctgtagcc gtagacgacc
     3241 cacagcacgc tgatcaggcc ggtgatggcg aagcactgca tcatcaccga caggaggttc
     3301 ttggcgcgga ccatgccgcc gtagaacagc gccaggccgg gaatggtcat cagcaacacc
     3361 agcacggttg ccgtcagcat ccatgcggta tcgccactgt tgagagtcac ttcttcggca
     3421 gccagggcca aacctgggct ggcgagagac aacagggctc ctagccctgc gattttgcgc
     3481 agagtcattg tgttactcct ggggcgtggg ggttcggtgg agtagtagtg taggtagcgc
     3541 ggtgcggctt agatcgcgtc ggtgccggtt tcgccggtac ggatgcggat cgcctgctcc
     3601 aggctgacca cgaagatctt gccgtcgccg atcttgccgg tgttggccgc cttggtgatg
     3661 gcgtcgatga ccgagtccag ctgatcgtcg gcgatcgcca cctcgatctt cactttcggc
     3721 aggaaatcga ccacgtattc ggcaccgcgg tacagctcgg tgtgcccctt ctgccggccg
     3781 aagcccttga cctcggtaac ggtgatgccc tgtacgccga tttccgacag cgactcgcgc
     3841 acgtcgtcga gcttgaacgg cttgatgatg gcagtgacta gcttcatgaa agtttctccc
     3901 gtaatggtga acttgcccca ggaacaacga acccacgaca agtctaagcg cagaatcc
//
LOCUS       DATCUN010000186         4334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_45814, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000186 DATCUN010000000
VERSION     DATCUN010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45814"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..113
                     /locus_tag="VIO83_07455"
     tRNA            37..113
                     /locus_tag="VIO83_07455"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:71..73,aa:Asp,seq:gtc)
     gene            complement(539..1495)
                     /locus_tag="VIO83_07460"
     CDS             complement(539..1495)
                     /locus_tag="VIO83_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY8331147.1"
                     /translation="MHRLLPSLALAGLIAAYGAWSQQRPVGHYLSDLRSQIISEQRPA
                     AARGNLLVIEPALNPHDYQSIARLHLKLAAHLQQARQAGLLTAQTLAVFPEHIGSGLL
                     LLGEKRQVYQARSLPQAMRWLVASNPLDFALALLQTEGSPRAVDALLRMKAQDMADAY
                     QQLFAGLAREFAIPLVAGSIILPQPQIRDGRLLAGNGPLYNVSLVFAADGQLLGAPQY
                     KQQAEAFEAQLLNGSAPPPQAVATPLGRLGVLLGADADNPQLLARLQAQQIELLVLPR
                     FRGETAGMGKVADANWRGQLWDFPPQGARTHSGAQLHNHWLD"
     gene            1775..3163
                     /locus_tag="VIO83_07465"
     CDS             1775..3163
                     /locus_tag="VIO83_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEY8331148.1"
                     /translation="MSLQKKPQMVEAVMFFNERGICKEMLYPEFEAMLDGVVNLPEFT
                     DQQMRAAFVLINPRLLVKSVVCFYLDFGGKGEADRGWNIPLRHLAERGGSGPDLGVGP
                     IRLACRSQCPVSWHQMHLWDPQLAPGHNDLLLIRDAVKRNNLGVLVEDETAQAAVQPA
                     KLHMAPEHKWYMQPDDLDLIRSGEEARKNERAEQEQRLKAAQLLKQQRLRITSLTQQH
                     EEELARLRLQTAQREKALQERLAALEQELAEQARRNDDLKARLTAQAESFQQVRVEMD
                     RQLHSLAHDGRSEIDGLRAQFEQEAQARINAALLEYQERLARQDAQLASQSQLDAQLR
                     AELAQLTEERDRLGREGGEQTLERLAAQGVVFVAYHPGAGHLTIPLQDIVVYQRNPMA
                     YVAAKCFVSEAQYSQWLAHYQQPSCFAQLPNGNRCNMPIDRVESPSRFVSGESDCCAR
                     HRASVRQRNLGS"
     gene            3163..4107
                     /locus_tag="VIO83_07470"
     CDS             3163..4107
                     /locus_tag="VIO83_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY8331149.1"
                     /translation="MLAADWPPPARLQAVCCGWLQRAGVELALLRLDLIDLLISGNKW
                     YKLAPHLAEARRLGAPGLISLGGAHSNHLHALAAAGARFGLATVGLLRGHEQETPTVA
                     DLRRFGMQLHWLGYAGYRDRHREDFWNPWRVLYPGFVPVAEGGLSLAAAQSCATLVDE
                     IERALPGLVWTGYDQLWAAAGSGTTLAGLVLGERGRHPVLGTLAVPADHGVASALERL
                     LDEAGVAAGQGYRLLDASRGGFARLDGELARFILDFEHETGVPLEPLYTGKLLLALQE
                     AVARGEIARGSRIVAVHSGGLQGRRALESRLAELASQS"
     gene            complement(4123..>4334)
                     /locus_tag="VIO83_07475"
     CDS             complement(4123..>4334)
                     /locus_tag="VIO83_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEY8331150.1"
                     /translation="PLARLRWLAPSACSADELWSAAQLDAWLDELPADAAPRMLVHLT
                     EAADGRWQERERVFLVNDRWPLPPQ"
BASE COUNT          715 a         1397 c         1488 g          734 t
ORIGIN      
        1 tttacccagt tgtaccggac gaaagtccac cgacgcgcag cggtagttca gtcggttaga
       61 ataccggcct gtcacgccgg gggtcgcggg ttcgagtccc gtccgctgcg ccatatcgaa
      121 aagccctctg gcagtcatgc cagacggctt tttactttct gcaatattca tttattcgtc
      181 atcctggctc tttaggctga tactcgtagt cacaagacat cgaagaaatc agtcaagaag
      241 aggaatgccc atggacgact atcaggaaga actgctggat ctgcaggctg ccgaacgtga
      301 cctgcctgaa cccgcggaag atgccaccga gttgtgatca acccactcgt cgctggttgc
      361 gacgaaaatc atcgggcgtc tgcccgagag cgcgtttgaa ggcccgctaa acggtttgcg
      421 ccaaggcgaa accaggcagg ccatccactt gaacgtcaga tctacatccc cccctgccac
      481 ttgccgccag ctcgcgaccc tgtcgccgag tgctggcgga tctgcgtgcg cacgaacctc
      541 agtccagcca atggttgtgc agctgcgcgc cgctgtgcgt gcgtgcgcct tgcggcggga
      601 aatcccacag ctggccacgc cagttggcat ccgcaacctt gcccatgccc gccgtctcgc
      661 cacgaaaacg tggcagcacc agcagctcga tctgctgcgc ctgcaggcgg gcgagcagct
      721 gcggattgtc ggcgtcggcg ccgagcagca cgccgaggcg gccgagcggg gtggcgacgg
      781 cctgtggcgg cggggcggag ccgttcagca actgcgcttc gaaggcttca gcctgctgct
      841 tgtactgggg ggcaccgagc aactggccgt cggcggcgaa caccagactg acattgtata
      901 gcgggccgtt gccggccagc aggcggccat cgcggatctg cggctgcggc aggatgatcg
      961 agccagccac cagcgggata gcgaattcgc gggccagccc ggcaaacagc tgctggtagg
     1021 cgtcggccat gtcctgcgct ttcatgcgca gcaaggcgtc gacggcgcgg ggcgagcctt
     1081 cggtctgcag cagggcgagg gcaaaatcca gcggattgct ggctaccagc cagcgcatgg
     1141 cctggggcag gctgcgtgcc tggtagacct ggcgtttctc gccgagcagc agcaggccgc
     1201 tgccgatgtg ttcggggaac actgccagtg tctgtgccgt gagcaggcca gcctggcgtg
     1261 cctgctgcag gtgagccgcc agcttgaggt gcaggcgcgc gatactctgg tagtcatggg
     1321 ggttgagagc cggctcgatg accagcagat tgccgcgggc cgcagcgggt cgttgctcac
     1381 tgatgatctg gctgcgcaga tcggacaggt agtggccaac cggacgttgc tggctccacg
     1441 cgccataggc cgcgatcagg cccgccagcg ccagggatgg caacagtctg tgcatggaaa
     1501 actcctttca gcagctgtca gcctagggga ggtgggcggg cttgccaatc tggcgcctgg
     1561 cttgaggcgc taccgtgtca cagtttgggc gcgacggacg gtcctgtgcg cagactggcc
     1621 ggtagcctgc caacctcttc acattgctgg gcggcgcttt tctcctagac tcggcccata
     1681 taaggttggg cgcatgcatt tttcatgcgc cagatcatgg accgcttgtt caagcgccgg
     1741 gcgctgggca atcaggcctg catgaggaaa gtcgatgagc ctgcagaaaa agccgcagat
     1801 ggtagaagcc gtgatgttct tcaacgagcg tggcatctgc aaggaaatgc tctatccgga
     1861 gttcgaggcc atgctggacg gcgtggtcaa cctgccggag ttcaccgacc agcagatgcg
     1921 tgcggccttc gtactgatca atccgcgcct gctggtcaaa tcggtagtgt gtttctatct
     1981 ggactttggt ggcaaggggg aggccgatcg tggctggaac attcccctgc gtcacctggc
     2041 cgagcggggc gggagcggcc ccgatctggg cgttggacca atccgcttag cctgtcgcag
     2101 ccagtgcccg gtgtcctggc accagatgca cctgtgggat ccgcagctgg cgccggggca
     2161 caatgacctg ctgttgatcc gtgacgccgt caagcgcaac aacctcggcg tgctggtcga
     2221 agacgagacc gcgcaggcgg cggtgcagcc ggccaagctg cacatggcgc ccgagcacaa
     2281 gtggtacatg cagcctgacg atctcgattt gatccgctcg ggcgaggaag cgcgcaagaa
     2341 cgagcgggcc gaacaggagc agcgcctcaa ggcggcccag ctgctcaaac agcagcgtct
     2401 gcgcatcacc agcctgaccc agcagcatga ggaggagctg gcgcgtctgc ggctgcagac
     2461 tgcgcaacgc gagaaagccc tgcaggagcg cctggcggcg ctggagcagg agttggccga
     2521 acaggcccgg cgcaacgacg atctgaaggc gcgtctcacc gcccaggcgg agagcttcca
     2581 gcaggtccgc gtggagatgg atcgccagtt gcattccctg gcgcatgacg gacgcagcga
     2641 gatcgacggc ctgcgtgccc agttcgagca ggaagcccag gcgcggatca acgcggcgct
     2701 gctcgagtac caggagcgtt tggcccggca ggatgcccag ctcgccagcc agtcccagct
     2761 ggacgcgcag ttgcgcgccg agctggcgca actgaccgag gagcgcgacc gtctggggcg
     2821 cgagggcggc gagcagacac tcgagcgcct ggcggcgcag ggcgtggtgt tcgttgctta
     2881 ccatccgggc gctggtcacc tgaccattcc gctgcaggac atcgtcgtct accagcgcaa
     2941 ccccatggcc tatgtggcgg ccaagtgctt cgtcagcgag gcgcagtaca gccagtggct
     3001 ggcgcattac cagcagccga gctgcttcgc ccagttgccc aacggcaacc gctgcaacat
     3061 gcccatcgac cgggtcgagt cgcccagccg cttcgtcagt ggcgagtccg actgctgcgc
     3121 ccgacaccgc gccagtgtgc gccagcgcaa tctcggcagc tgatgctggc cgctgactgg
     3181 ccgccgccgg cgcggctgca ggcggtgtgc tgtggctggc tgcagcgcgc cggtgtcgag
     3241 ctggcgctac tgcgcctgga cctgatcgat ctgctgatca gtggcaacaa gtggtacaag
     3301 ctcgcccccc atctggccga ggcccgccgc cttggtgcgc ccggactgat cagtctgggc
     3361 ggtgcccact ccaaccatct gcacgccctg gccgccgccg gtgcccgctt cggtcttgcc
     3421 accgtcggcc tgctgcgcgg ccatgagcag gaaaccccca ccgttgctga tctgcgccgc
     3481 ttcggcatgc agctgcactg gcttggctac gccggctatc gcgaccgtca tcgggaagac
     3541 ttctggaatc cctggcgagt gctttatccg ggtttcgtgc cggtcgccga aggcggcctg
     3601 agcctggcgg cggcgcagtc ctgcgcgacc ctggtcgacg agatcgagcg cgcgctgccc
     3661 gggctcgtct ggaccggtta cgaccagctg tgggccgcgg ccggcagcgg caccaccctg
     3721 gccggcctgg tgctgggcga gcgcggtcgc catccggtac tcggcaccct ggcggtgccg
     3781 gccgatcatg gcgtggcgtc ggcgctggag cgtctgctcg acgaggccgg cgtcgcggca
     3841 ggacagggct accggctgct ggacgccagt cgcggcggtt tcgcccgcct ggatggcgag
     3901 ctggcccgct tcatcctcga cttcgagcac gaaaccggtg ttccgctcga gccgctgtat
     3961 accggcaagc tgctgctggc gctgcaggag gcggtggcac gcggcgagat tgcccgcggc
     4021 agccgcatcg ttgcggtgca cagtggcggc ctgcaggggc gccgggcgct ggagtcacgg
     4081 ctggccgagc tggccagtca gtcctgagtt ttcgtggcgc ggtcactgcg gcggcagcgg
     4141 ccagcgatcg ttgaccagaa agacccgctc gcgctcctgc cagcgtccgt cggcagcctc
     4201 cgtcaggtgc accagcatgc gcggtgcggc gtcggcgggc agttcgtcca gccaggcgtc
     4261 cagctgcgcg gcgctccaga gctcgtcggc gctgcacgcg ctcggcgcca gccagcgcag
     4321 gcgcgccagg ggct
//
LOCUS       DATCUN010000187         3147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_120679, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000187 DATCUN010000000
VERSION     DATCUN010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120679"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VIO83_07480"
     CDS             complement(<1..383)
                     /locus_tag="VIO83_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020306893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331151.1"
                     /translation="MKEQLIELITLISSGCMRDEEIARIADEAAQAYADPQAFLAANP
                     DVNYGDDFPIPLGEWVVVGSLPDTVLFLGDSPSELFQQIRDSFGPGVPFVLTAKQLAK
                     ADLLSALRRMQVQMSALYPEKGGYVL"
     gene            625..1929
                     /locus_tag="VIO83_07485"
     CDS             625..1929
                     /locus_tag="VIO83_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8331152.1"
                     /translation="MTTTSALTPLLMSAAPLAPDMSISDVADRLLTPECKAFLSLAVV
                     DADGRPQGLISRYALQNIFMLRFGRDLHGQKPAHSVMNDAPLVVCLDTRLEEAAQQVT
                     SKLTYPITEDFILVDGEGRYQGLGTVLDLLKAMEKRVAQRNAVLRRALVDLKESQAQL
                     VQSEKMASLGQMVAGVAHELNTPLGYVKNNVQLLRELKQPLLQLADTHGALLDCLHAE
                     DCDEARLAAALGEAVDAREQAAPELLKEDLQQLFSDTLYGLEQIGELVVGLKNFARLD
                     RAMSEDIDLNDCVRSALVISRNNIKDKAEVTAQLGDLPRIPCAPSQINQVLLNLLTNA
                     AQAMERFGQILVKTWADNDAVFISVQDSGKGMPPEVLAKIFDPFFTTKPVGQGTGLGL
                     SISFKIIQDHGGTIRVASEPGRGTRFLIRLPRAQAPNLQESA"
     gene            1931..2842
                     /locus_tag="VIO83_07490"
     CDS             1931..2842
                     /locus_tag="VIO83_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8331153.1"
                     /translation="MTAPTRILFVDDEERILRSLAMQFRRQYDVLTESDPRRALERLK
                     SESVQIIVSDQRMPQMSGAEFLAQASEIAPHTLRILLTGYSDLDAAVDALNSGGIFRY
                     LTKPWDQQEMTNTLRQAAGIAASQAPAALTDSSEPTTALTVLLLDDHAETLAAVGSFC
                     HAGGHRLLHARNLPEALQQLNSEAVDLLISDLKLAGEDTAPLLKTLAQAHPRLLSLVV
                     TPFQDTQALLRLINEAQIFRYLPKPIRPALFEKSLSAAARQAQAWRAQPTHVVARLAE
                     APKEERERDKVGSLMGMLGRLRERLIA"
     gene            complement(2864..>3147)
                     /locus_tag="VIO83_07495"
     CDS             complement(2864..>3147)
                     /locus_tag="VIO83_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331154.1"
                     /translation="GAVALRYWSLSEAGYIPVSNHGPGVQILTAGGLGAAGGSGGKGG
                     VAGAGEFSTRGNGIKVYRNGGAPGEAGLTGAPGSSGETGRFLVQPQARP"
BASE COUNT          560 a         1144 c          990 g          453 t
ORIGIN      
        1 agcacgtaac cgcccttctc cggatacagc gcgctcatct gcacctgcat gcgccgcagg
       61 gcgctgagca ggtcggcctt ggccagctgc ttggcggtca gcacgaaggg cacgcccgga
      121 ccgaaggagt cgcggatctg ctggaacagt tcgctggggc tgtcgcccag gaacagcacg
      181 gtatccggca ggctgccgac caccacccac tcgccgagcg gaatggggaa gtcgtcgccg
      241 tagttgacgt cggggttggc agcgaggaac gcctgcggat cggcataggc ctgggctgcc
      301 tcgtcggcga tgcgcgcgat ctcttcgtcg cgcatgcagc ccgaactgat cagggtgatc
      361 agctcaatca gctgttcttt catggagagg tcctgtggga gccaagaaaa ttcgccggca
      421 ttttcgccga tgcggcgctc cggcggggag cttattcgcc gccccggcgc cattcccgac
      481 cggccaccga cagatcgcag agcgccagaa ggcgcaagcc gggcaccgcg ggagtgtgcc
      541 gcgctgcgca aaaccaccgg taacaccatc gcaaccttgc gctgcttcaa tggcggccac
      601 tggataggac gttgagagtc atgcatgacc accactagcg ctctgacacc gctgctgatg
      661 tccgcagcgc cgctggcccc cgacatgagc atctcggatg tggccgaccg cctgctgacc
      721 cccgaatgca aggccttcct gtccctggcc gtggtcgacg ccgacggccg cccgcagggc
      781 ctgatcagcc gctatgccct gcagaacatc ttcatgctgc gcttcggccg cgacctgcac
      841 gggcagaagc cggcccacag cgtgatgaac gacgcgccac tggtggtctg cctggacacc
      901 cgcctggaag aggcggccca gcaggtcacc tcgaagctga cctacccgat caccgaggac
      961 ttcatcctgg tcgacggcga gggccgctac cagggtctgg gcacggtgct cgacctgctc
     1021 aaggccatgg aaaagcgcgt cgcccagcgc aacgcggtgc tgcgccgggc gctggtcgac
     1081 ctcaaggaat cccaggccca gctggtgcag tcggagaaga tggcctcgct gggccagatg
     1141 gtcgccggcg ttgcccacga gctgaacacc ccgctgggtt acgtgaagaa caacgtgcag
     1201 ctgctgcgcg agctgaagca gccgctgctg caactggccg acacccacgg cgccctcctc
     1261 gactgcctgc acgccgagga ctgcgacgag gcgcgcctgg ccgctgccct gggcgaggcg
     1321 gtcgacgccc gcgaacaggc ggcgccggag ctgctcaagg aagacctcca gcagctgttc
     1381 agcgacaccc tctacggcct cgagcagatc ggcgaactgg tggtcggcct gaagaacttc
     1441 gcccgcctcg accgcgccat gagcgaggac atcgacctca acgactgcgt gcgcagcgcc
     1501 ctggtgatct cccgcaacaa catcaaggac aaggccgagg tcaccgcgca actcggcgat
     1561 ctgccgcgca ttccctgcgc gccgtcgcag atcaaccagg tgctgctcaa cctgctgacc
     1621 aacgccgccc aagccatgga gcgcttcggc cagatcctgg tgaagacctg ggccgacaac
     1681 gacgccgtgt tcatttccgt gcaggactcc ggcaagggca tgccgcccga ggtgctggcg
     1741 aagatcttcg atcccttctt caccaccaag ccggtcggcc agggcaccgg cctcggcctg
     1801 tccatcagct tcaagatcat ccaggaccat ggcggcacga ttcgcgtcgc ctccgagccg
     1861 gggcgaggca cacgcttcct gatccggctg ccgcgcgcgc aagcgcccaa cctgcaggag
     1921 agtgcctgat atgaccgccc ccacccggat cctcttcgtc gacgacgaag agcgcattct
     1981 gcgcagcctg gccatgcagt tccgccgcca gtacgacgta ctcaccgaaa gcgacccgcg
     2041 ccgcgcgctg gaacgcctga agagcgagtc ggtgcagatc atcgtcagcg accagcgcat
     2101 gccgcagatg agcggcgcgg agtttctcgc ccaagccagc gagatcgccc cgcacaccct
     2161 gcgcatcctg ctcaccggct actccgacct cgacgccgcg gtcgatgcgc tgaacagcgg
     2221 cggcatcttc cgctacctga ccaagccgtg ggatcagcag gagatgacca acaccctgcg
     2281 ccaggcggcg ggaatcgccg ccagccaggc ccctgcggca ttgaccgaca gctcggagcc
     2341 gaccaccgcg ctgaccgtac tgctgctcga cgaccatgcg gaaaccctgg ccgcggtcgg
     2401 cagtttctgc cacgccggcg gccatcgcct gctgcacgcc cgcaacctgc ccgaagccct
     2461 gcagcagctg aacagcgaag cggtcgacct gctgatcagc gacctcaagc tggcgggcga
     2521 agataccgcg ccgctactca agaccctggc ccaggcgcat ccgcgcctgc tcagcctggt
     2581 ggtcacgccg ttccaggaca cccaggcgct gctcaggctg atcaacgagg cgcagatctt
     2641 ccgctacctg cccaaaccga tccgcccggc cctgttcgag aagagcctga gcgcggccgc
     2701 ccgtcaggcc caggcctggc gggcacaacc gacccatgtg gtggcgcgcc tggccgaagc
     2761 cccgaaagag gaacgcgaac gcgacaaggt cggcagcctg atgggcatgc tcggccgcct
     2821 gcgcgagcgg ctgatcgcct gaggccggct gaacgccggt cggtcagggc cgcgcctgcg
     2881 gctgcaccag gaagcgtccc gtctcgccgc tgctccccgg cgcgccagtc agacctgcct
     2941 cgcccggcgc gccgccattg cgatagacct tgatgccgtt gccacgggtg ctgaattcgc
     3001 cggcaccggc aaccccgccc ttgccgccac tgccgccagc ggcgcccagg ccaccggcgg
     3061 taaggatctg cacgcccgga ccatggttgc tgaccgggat atagccggcc tcgctcaggc
     3121 tccagtagcg cagcgccacc gcgccac
//
LOCUS       DATCUN010000188         5007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_233291, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000188 DATCUN010000000
VERSION     DATCUN010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233291"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..1579)
                     /gene="dctM"
                     /locus_tag="VIO83_07500"
     CDS             complement(296..1579)
                     /gene="dctM"
                     /locus_tag="VIO83_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter large permease
                     protein DctM"
                     /protein_id="HEY8331155.1"
                     /translation="MTILFLFLALFVLMFIGVPIAVSLGLAGSVTIMLFSPDSVRSLA
                     IKLFETSEHYTLLAIPFFLLAGAFMTTGGVARRLIDFANACVGHIRGGLAIGAVFACM
                     LFAALSGSSPATVAAVGSIAIAGMVRSGYPQAFGAGIVCNAGTLGILIPPSVVMVVYA
                     AATETSVGKLFMAGVVPGILLGAVLMVAIYIIAVKKNLPALPRASVREWLSAARKAVW
                     GLLLMVIILGGIYSGMFTPTEAAAVAAVYSGFVALFVYKDMSLRECPKVILESAKLSI
                     MLMFIIANAMLFAHVLTTEQIPQTITAWVIDMGLSPWMFLLVVNIVLLVAGAFMEPSA
                     IILILAPILFPIAVQLGIDPIHLGIIMVVNMEIGLITPPVGLNLFVTSAVTGMPVSAV
                     IRAAMPWLMMLLGFLLIITYVPAVSLALPEWLGMS"
     gene            complement(1576..2208)
                     /locus_tag="VIO83_07505"
     CDS             complement(1576..2208)
                     /locus_tag="VIO83_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY8331156.1"
                     /translation="MNALRLVWDRFEEGFIVFLLAAMTLVTFVYVILNNLYTLFYNLA
                     ERFEGASEALFAIGDAILDLAQAMTWSNALTKALFAWLIFFGLAYGVRTAGHIGVDAL
                     VKLASKPVQRLIGLVGCLFCLGYAGLLSVASFEWVKTLFDAAIGAEDLGHFGVMQWHI
                     GLIVPVGFAMVFIRFAEIFVRILRNQQTGLGLADEAAEAVKLSDHQELNK"
     gene            complement(2324..3319)
                     /gene="dctP"
                     /locus_tag="VIO83_07510"
     CDS             complement(2324..3319)
                     /gene="dctP"
                     /locus_tag="VIO83_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP substrate-binding protein
                     DctP"
                     /protein_id="HEY8331157.1"
                     /translation="MFKLTAKALACALSLTIAGIAQAADPIVIKFSHVVAEHTPKGQG
                     ALLFKKLAEERLAGKVKVEVYPNSSLFGDGKEMEALLLGDVQIIAPSLAKFEHYAKPI
                     QIFDLPFLFDNIQAVDRFQNSAAGKSLLTSMEGKGITGLGYWHNGMKQLSANKPLYEP
                     KDARGLKFRVQASQVLEEQFKAVRANPRKMSFAEVYQGLQTGVVNGAENPYSNIYSQK
                     MHEVQKYITETNHGVLDYMVITNTKFWSGLPEDVRSELGKIMAEVSVEVNKQSEALNQ
                     GDKQRILDAKTTEIIALNDEQRGKWRDAMKPVWKKFEADIGADLIKAAEAANAAQ"
     gene            complement(3622..5004)
                     /locus_tag="VIO83_07515"
     CDS             complement(3622..5004)
                     /locus_tag="VIO83_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014822149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8331158.1"
                     /translation="MSSDNLISADAQVLLIDDDPHLRKALSQTLDLAGLRVVSLGDAR
                     EVGTRLPADWPGVVVSDIRMPGLDGLALLEQLRGRDAELPVLLITGHGDVPLAVQAMR
                     AGAWDFLEKPFPSDTLLDSVRRALAMRRLVLENRQLRQTLSGRDALEGRLIGLSPEIV
                     RLRQQVAALARTNADVLILGETGSGKEVVARALHDLSARRDGPFVAINAGALAESVVE
                     SELFGHEAGAFTGAQKRRIGKFEFANGGTLFLDEIESMSLEVQVKLLRLLQERAVERL
                     GSNQLIPLDIRIIAATKEDLRQAADQGRFRADLYYRLNVAPLRIPPLRERGDDVRLLF
                     RHFCEAAANRHGLPLREPDASQSAALLTHNWPGNVRELQNVAERFALGLDLGLGEVLA
                     ELPAEGNLAARVEAFERSLITTELAHGHSSLRSLAEALGVPRKTLHDKLRKHGLSLGE
                     SGGNPHDDLD"
BASE COUNT          807 a         1630 c         1803 g          767 t
ORIGIN      
        1 tcccagcgct cgatctgctc ggccagcgcc ggcagttcgc gggcggcgcg ctccaccggc
       61 cagtccgggc aggcggcgtg ggcaaggggg atgacaggca gtgccagcgc cacgagggca
      121 gccggcagcg cgcgcagcag gcggcggggc atggggtgag cgtccttgct cgggagagag
      181 cggccagctt agggggatcg gcggaggccg gctgtgcgct ggcgccgctt ttcgccgggc
      241 aaaaaaagac ccggcagcgc cgggtcgaat gcctttcaag cctaccgcgg aaaactcagc
      301 tcatccccag ccactcgggc agcgccagcg acaccgccgg cacgtaggtg atgatcagca
      361 ggaagcccag cagcatcatc agccacggca tggcggcgcg gatcaccgcg ctgaccggca
      421 tgccggtgac cgccgaggtg acgaacaggt tgaggccgac cggcggggtg atcaggccga
      481 tctccatgtt caccaccatg atgatgccga ggtggatcgg gtcgatgccc agctgcacgg
      541 cgatcgggaa caggatgggg gcgaggatca ggatgatcgc cgacggttcc atgaaggcgc
      601 cggccaccag cagcacgatg ttcaccacca gcaggaacat ccacggcgac aggcccatgt
      661 cgatcaccca ggcggtgatg gtctgcggga tctgctcggt ggtcagcacg tgggcgaaca
      721 gcatggcgtt ggcgatgatg aacatcagca tgatgctcag cttggccgac tcgaggatca
      781 ccttcgggca ctcgcgcagg ctcatgtcct tgtagacgaa cagggcgacg aagccggagt
      841 acaccgcggc caccgccgcg gcctcggtgg gggtgaacat gcccgagtag atgccgccga
      901 ggatgatcac catcagcagc aggccccaca ccgccttgcg cgcggcggaa agccactcgc
      961 gcacggaggc gcgcggcagc gccggcaggt tcttcttcac cgcgatgatg tagatcgcca
     1021 ccatcagcac cgcgccgagc aggatgcccg gcaccacgcc ggccatgaac agcttgccga
     1081 ccgaggtctc ggtggcggcg gcgtacacca ccatcaccac cgacggcggg atgaggatgc
     1141 cgagggtgcc ggcgttgcag acgatgccgg cgccgaacgc ctgcggatag ccggaacgca
     1201 ccatgccggc gatggcgatg gagccgaccg ccgccacggt ggccggcgag gagccggaca
     1261 gcgcggcgaa cagcatgcag gcgaacaccg caccgatggc caggccgccg cggatgtggc
     1321 cgacgcaggc attggcgaag tcgatcagcc ggcgggccac gccgccggtg gtcatgaagg
     1381 cgccggcgag caggaagaac gggatcgcca gcagggtgta gtgctcggac gtctcgaaca
     1441 gcttgatcgc cagcgagcgt accgagtccg ggctgaacag catgatggtg accgaaccgg
     1501 ccaggccgag ggacacggcg atcggcacgc cgatgaacat cagcacgaac agggcgagga
     1561 acaggaacag gatggtcatt tgttcagctc ctggtgatcg ctcagcttga ccgcctcggc
     1621 ggcctcgtcg gccaggccca gaccggtctg ctggttgcgc aggatgcgca cgaagatttc
     1681 cgcgaagcgg atgaacacca tggcgaagcc gaccggcacg atcaggccga tgtgccactg
     1741 catcacgccg aagtggccga ggtcctcggc gccgatggcg gcgtcgaaca gggtcttgac
     1801 ccactcgaag ctggccacgc tgagcaggcc ggcgtagccc aggcagaaca gacagccgac
     1861 caggccgatg aggcgctgca ccggcttgct ggccagcttg accagcgcgt cgacgccgat
     1921 gtggccggcg gtgcgcacgc cataggccag gccgaagaag atcagccagg cgaacagcgc
     1981 cttggtcagc gcgttgctcc aggtcatcgc ctgggccagg tcgaggatgg cgtcgccgat
     2041 ggcgaagagc gcttcgctgg cgccctcgaa gcgctcggcc aggttgtaga acagcgtgta
     2101 caggttgttg aggatgacgt agacgaacgt caccagggtc attgccgcca gcaggaagac
     2161 gatgaagccc tcctcgaagc gatcccagac cagacgcaag gcgttcatgg ggtatctccg
     2221 ctgagaaagc gcggggcggg ccccctgcag gcgtgcgctg tgcagggggc caccggcccg
     2281 gcgcgagccg ggagcgaccg gggtcgctcg gtggtgcgag tgcttactgg gctgcgttgg
     2341 cggcttcggc ggccttgatc aggtcggcac cgatgtcagc ctcgaacttc ttccacaccg
     2401 gcttcatcgc atcgcgccac ttgccgcgct gctcgtcgtt gagggcgatg atctcggtgg
     2461 tcttggcatc gaggatgcgc tgcttgtcgc cctggttgag ggcttccgac tgcttgttca
     2521 cctcgacgct gacctcggcc atgatcttgc ccagctcgct gcgcacgtcc tccggcaggc
     2581 cgctccagaa cttggtgttg gtgatcacca tgtagtcgag cacgccgtgg ttggtctcgg
     2641 tgatgtactt ctgcacctcg tgcatcttct gcgagtagat gttcgagtag gggttctcgg
     2701 cgccgttgac cacgccggtc tgcaggccct ggtagacctc ggcgaagctc atcttgcgcg
     2761 ggttggcgcg caccgccttg aattgctcct cgagcacctg cgaggcctgc acgcggaact
     2821 tcaggccgcg ggcgtccttc ggctcgtaca gcggcttgtt ggccgacagc tgcttcatgc
     2881 cgttgtgcca gtagcccagg ccggtgatgc ccttgccttc catcgaggtc agcaggctct
     2941 tgccggcggc gctgttctgg aagcggtcga ccgcctggat gttgtcgaac aggaacggca
     3001 ggtcgaagat ctggatcggc ttggcgtagt gctcgaactt ggccagcgac ggcgcgatga
     3061 tctgcacgtc accgagcagc agcgcttcca tttccttgcc gtcgccgaac agcgaggagt
     3121 tcgggtagac ctcgaccttg accttgccgg ccaggcgctc ctcggccagc ttcttgaaca
     3181 gcagcgcacc ctggcccttc ggggtgtgct cggcgaccac gtgggagaac ttgatcacga
     3241 tcgggtcggc cgcctgggcg ataccggcga tggtgagcga gagggcgcag gcgagcgcct
     3301 tggcagtcag cttgaacatg cgggacatcc tcattgttat gtcatgcagc gtgaaaggcg
     3361 ttaccccggc ggcgagctgt ccggccggca cggacgccgc cgtgcgccgg cggacaggtc
     3421 gggcgcgaga tgccttgagc ttagcaaccg ctatgccagc gtgccgggag gccgccgcgc
     3481 cggggtgggg cgggcggcgt cagccgctct ggcgcgagcc ttgcaggcag gggttcgact
     3541 ttggtcgagg gttgtccggc cgcgccgcgc gcaggccgga cggcgtgcgc gggcgggaag
     3601 gggtggcggg attccgccgg gtcagtcgag gtcgtcgtgc ggatttccgc cactctcgcc
     3661 aaggctcagg ccgtgcttgc gcagcttgtc gtgcagggtc ttgcgcggca cgccgagggc
     3721 ctcggccagg ctgcgcagcg agctgtggcc gtgggccagc tcggtggtga tcaggctgcg
     3781 ctcgaaggcc tccacccgtg ccgccaggtt gccctcggcg ggcagctccg cgagcacttc
     3841 gccgaggccg agatcgaggc ccagggcgaa gcgctcggcg acgttctgca gctcgcgcac
     3901 gttgcccggc cagttgtgcg tcagcaacgc ggcgctctgg ctggcgtccg gctcgcgcag
     3961 cggcaagccg tggcggttgg ccgccgcctc gcagaagtgg cgaaacagca ggcgcacatc
     4021 gtcgccgcgc tcgcgcagcg gcgggatgcg cagcggcgcg acgttgaggc ggtagtacag
     4081 gtcggcgcgg aagcgcccct ggtcggcggc ctggcgcagg tcctccttgg tcgcggcgat
     4141 gatgcggatg tccagcggga tcagctggtt ggagccgagg cgctcgaccg cgcgctcctg
     4201 cagcaggcgc agcagcttga cctgcacctc gaggctcatg ctctcgatct cgtcgaggaa
     4261 cagggtgccg ccgttggcga actcgaactt gccgatgcgg cgcttctgcg cgccggtgaa
     4321 ggcgccggcc tcgtggccga acagctcgct ctccaccacc gactcggcca gcgcgccggc
     4381 attgatggcc acgaacggac cgtcgcggcg cgccgacagg tcgtgcagcg cgcgcgccac
     4441 cacctccttg ccgctgccgg tctcgccgag gatcagcacg tcggcgttgg tccgcgccag
     4501 cgcggcgacc tgctggcgca ggcgcacgat ctccggcgac aggccgatca ggcggccctc
     4561 cagggcgtcg cggccggaca gggtctggcg cagctggcgg ttttccagca ccaggcggcg
     4621 catggccagg gcgcggcgca cgctgtcgag cagggtatcg ctggggaaag gcttctcgag
     4681 gaagtcccag gcgccggcgc gcatcgcctg caccgccagc ggcacgtcgc cgtggccggt
     4741 gatcagcagc accggcagct cggcgtcgcg cccgcgcagc tgttccagca gcgcgaggcc
     4801 atcgaggccg ggcatgcgga tgtcgctgac caccacgccc ggccagtcgg ccggtagccg
     4861 tgtgcccacc tcgcgcgcat cgcccaggct gaccacccgc aggccggcca ggtccagggt
     4921 ctggctcagc gccttgcgca ggtgcgggtc gtcgtcgatc agcagcacct gggcgtcggc
     4981 gctgatcagg ttgtcgctgc tcattgc
//
LOCUS       DATCUN010000189        23523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_26891, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000189 DATCUN010000000
VERSION     DATCUN010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 23523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23523
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26891"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VIO83_07520"
     CDS             complement(<1..985)
                     /locus_tag="VIO83_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY8331159.1"
                     /translation="MSQFPHNERPLALRFPLHGSRLIEASAGTGKTFTISALYLRLVL
                     GHGGADGFARELLPPEILVVTFTDAATRELRDRIRARLVQAAKVFRDEEQGDDLLRQL
                     RGDFPAEQWPACARRLDIAAQWMDEAAVSTIHGWCQRMLREHAFDSGSLFTQTLETDH
                     GELMAEVVRDYWRQQCYPLAGAALGWVMNHWLHPDKLLKDKLRPLLGEADAAPTEALG
                     VLLERALETQARELAELKAPWPAWADELEALLDAAVAAKAVDGRKIQARYYQPWFAKL
                     REWAAGDEAALDIGTGFTRLTPDGLAEAWKKGEPPQHAALEAMAGLKGRLDA"
     gene            complement(982..4425)
                     /gene="recC"
                     /locus_tag="VIO83_07525"
     CDS             complement(982..4425)
                     /gene="recC"
                     /locus_tag="VIO83_07525"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEY8331160.1"
                     /translation="MQGNELTPGLMVVHGNRLEELRELAVQWMRLHPLAPLENEVVLV
                     QSNGIAQWLKLALAAQDSCGIAAAIQVELPARFLWQTYRAVLGSESIPLESPLDKAPL
                     TWRLMRLLPNLLAGEAFAPLRRFLADDSDLRKRHQLAERLADLFDQYQVYRADWLADW
                     AAGLDQLRTSRNGAKALDEAVRWQPALWRALLEDVGREQLGESRAGVHPRFVAHLRNV
                     DERPRGLPRRVIVFGISSLPAQMVEALAALARFSQVLLCVHNPCQHHWGDIVPDQDLL
                     RHQYRRQQHKGGMTPGLSADELHQHAHPLLAAWGKQGRDYINLLDQHDDPEHYRARFA
                     AINQRIDLFGEGDTAHLLGQLQDDILELRPLAETRDTWPELDPAKDQSLRFHIAHSAQ
                     REVEILHDQLLARFSADPSLRPREIIVMVPDVNAYAPHIQAVFGQYAQDDARYIPFTL
                     ADQGQRGCEPLLIAVEHLLKLPEGRLTVSEVLDLLDVPALRARFAIDESELPTLHRWI
                     EGAGIRWGLDAERRASLGLPDGLEQNTWRFGLRRMLLGYAVGSGAACAGIEPYDEIGG
                     LDAALIGPLVKLLDALDALHRRLARSATPSEWGTTLRALLADFFVAQNERDETLLGQL
                     VEALDSWLELCAGVELTEPLPLTVVREAWLGGLDQGRLSQRFLAGAVNFCTLMPMRAI
                     PFRMVCLLGMNDGDYPRSVTPLDFDLMAGDYRPGDRSRREDDRYLLLEALLAAREQLY
                     VSWVGRSIRDNSERPASVLVGQLRDHLGSGWTLTGGGDLLHALTTEHPLQPFSRDYFA
                     GGSELFSYAREWADLHRQSAQAAEVGALPPFQADAPLALETLQNFLRDPVRHFFTQRL
                     KVHLDEEVQAQLDEEPFGLDGLQLYQLRQELLGAATLALEEGPEQVEPALQATALRLQ
                     RSGQLPLAGFGERLREQVLEPLPAQMQRYQALCALWPDRLDSPRQLPFAHAGVELDGW
                     LGGLRSRGGEALARIELQPGELKSKDKGWKWHRLLRPWVAHVAAAACGVPLTTLLVGE
                     DVRLEFQPVAQDEARQILQRWLEGWRDGLNAPLPVALQTAIAWLSAEDEIKAAKAASE
                     AYEGGYQRTGEVEQSASLARQYPDFAALTVDGDFYQWAHDLYQPLLKADIRELKEEAR
                     A"
     gene            4518..4754
                     /locus_tag="VIO83_07530"
     CDS             4518..4754
                     /locus_tag="VIO83_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HEY8331161.1"
                     /translation="MKQPISGYHRDDENHWVAELACGHNQHVRHNPPWVSRPWVTTAA
                     GRERMLGYELQCRKCDAGAPPDRPQAPDGPDSAP"
     gene            complement(4857..5573)
                     /locus_tag="VIO83_07535"
     CDS             complement(4857..5573)
                     /locus_tag="VIO83_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEY8331162.1"
                     /translation="MTRLIRSLWLAASLAAFSSPLLASSPSAAPLIDSLAKAAPTLNH
                     EVLRHALAAMQCAVNNGSAPADRLAVIDFSLPSSERRLWVFDLKRKTLLLRELVAHGQ
                     GSGENFATSFSNREGSHQSSIGLFRTSETYHGQHGYSLRMDGLEPGINDRARERAIVI
                     HPADYVDPAWVKRHGRIGRSHGCPAVRPEVARMVIDNLKGGQFMFSWYPDRKWMQSSA
                     YLNCKPQRVAAILASRDSDS"
     gene            5815..7410
                     /locus_tag="VIO83_07540"
     CDS             5815..7410
                     /locus_tag="VIO83_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY8331163.1"
                     /translation="MLAMLLVSCQLAVAGAVAGESDNALQAELQQGQLSCASRPLPLA
                     ASARETLLDFYRQQGSERVWNRARLASLLTQLERLADDGLSPEAYHLADLRRLERISA
                     SDAQRTACREVLASHAYLQALQDLSWGRLDPAQVEPIWHSQHTPAPSRPSGAPAFATS
                     GLGDLPATFERARPQFKPYRNLRNAYARLRQQPLPAWPRIADGALLRPGKRDARVPLL
                     EQRLARAGYLTRTAGRPAGADSDLYDPLLVDAVETFQRDHQLQPDGVVGPATLAELNI
                     SAAARRDQVRVNLERLRWLAREMEPSMVLVDLAGTRVSYYRDRQLLWQARTQVGRAER
                     PSPLLKSRITHLTLNPTWTVPPTIFRKDKLPEIQRNIGYLAENRMRVLDNAGNELNPH
                     AVDWYNPQGILLRQDAGPTSALGVLAIRFPNPFSVYLHDTPSQQLFNKLPRVFSSGCV
                     RVENVLQLLDHLLADASPAERERIAAIHASGKTKNVNLPHAVTILLAYWTAEADDAGR
                     LQFRPDIYQHDAPILAALEAAER"
     gene            7524..8528
                     /locus_tag="VIO83_07545"
     CDS             7524..8528
                     /locus_tag="VIO83_07545"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY8331164.1"
                     /translation="MDLEQYLRALPKAELHLHIEGTLEPELMFALAERNGIALPWHSV
                     EETRAAYAFSDLQSFLDLYYAGAAALLREEDFFDLAMAYFERAHADGVVHAELFFDPQ
                     THTARGVAFATVFDGLERACSAARERYNISSRLILCFLRHLSEEDGFATLEQALPHLA
                     RIHGVGLDSSEQGHPPSKFVRLFARCRALGLHIVAHAGEEGPPAYIVEALDLLKAERI
                     DHGVRAAEDPALLQRLAREQVPLTVCPLSNVKLCVFPNLRQHNLKTLLDAGLKVTINS
                     DDPAYFGGYVLENYRQTALALQLSRDELKRIARNSLEAAFVDAVERAPWLARLEALAD
                     "
     gene            8598..9164
                     /locus_tag="VIO83_07550"
     CDS             8598..9164
                     /locus_tag="VIO83_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010954122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY8331165.1"
                     /translation="MNSKPAGERFRLYSAQQLDGVIEQMARQAAALFVGRREVVLVGI
                     LRRGEPLAQRMQSALVEHCGLPRWPLYPLHLKRYADDLSLLHAETALTENPELAALDL
                     SRTSVLLVDDVLYEGHSLLRASAWLAQLGAMETRTAVLVDRCVSRQPVRADITGLRLQ
                     VAPTDIIECNVPPYEREFGIEVLRRALN"
     regulatory      9238..9348
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      9351..9450
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9475..10092
                     /locus_tag="VIO83_07555"
     CDS             9475..10092
                     /locus_tag="VIO83_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEY8331166.1"
                     /translation="MLIYIYLIAITAEAMSGALAAGRRNMDMFGVGLIAFVTALGGGT
                     VRDILLGHFPVGWTQHPSYIYLTIGAGLFTMLVARFMHHLHKIFLVLDAMGLIAFTVI
                     GCNVALELNYSTPVVIMAGMITGVFGGILRDILCQRVPVVLRHELYASVSMLVAMLYL
                     LLNKLGINHDITLLASFGFGLALRLAAIRWSWSLPVFSYQPGRWE"
     gene            complement(10093..10758)
                     /locus_tag="VIO83_07560"
     CDS             complement(10093..10758)
                     /locus_tag="VIO83_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEY8331167.1"
                     /translation="MMRKAQIEVPIPDRVAAGKTLARLLDAYRNQPDTLVLALPRGGV
                     PVAWEIARELALPLDLMLVRKLGMPGHEEYAMGAIASGGVRVLNEEALLYGRVDAGTI
                     DRVTERQKSELERRDHLYRGNRPYPELQGCQVLLVDDGLATGSTMRAAVQAVRQQAPA
                     RIVLVAPVASTDAIAMLRPLVDEVVCPLVPDALSSIGEWYSDFGQTSDEEVQALLRQA
                     WAR"
     gene            11018..11506
                     /locus_tag="VIO83_07565"
     CDS             11018..11506
                     /locus_tag="VIO83_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HEY8331168.1"
                     /translation="MDKHKQGHKDTLLQPGHHDRDKEHQHLAGTAHCTGCGAYFHAGR
                     WSWQRQAGSHPEEITCPACRRIAENDAAGNVTLSGGFVQAHRDDLLNLIRNTEEGESK
                     EHPLERLIGISEEDDKVMVGTTGTHLANRIGHALEAAYDGEVSYTYTENETHLDVNWQ
                     RD"
     gene            complement(11523..12044)
                     /locus_tag="VIO83_07570"
     CDS             complement(11523..12044)
                     /locus_tag="VIO83_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003179003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY8331169.1"
                     /translation="MTAPIRLACILLVALLAGCSSQAPLPPQTPVSQAPAAYGPAEDV
                     LFRAIGLVGTPYRWGGNTPDSGFDCSGLIGYVYRDAAGVQLPRTTREMSALRAPQVAR
                     QHLQSGDLVFFATNGGRQVSHAGIYVGEDRFVHAPSAGGTVRLDSLNNSYWQQRYLDA
                     RRVLAGERLARNP"
     gene            complement(12157..12756)
                     /locus_tag="VIO83_07575"
     CDS             complement(12157..12756)
                     /locus_tag="VIO83_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY8331170.1"
                     /translation="MLKRFAPLVPLTLALFLAACASTSHQAQQPQQSFSSPSQQLSFE
                     LDAAAAAKAEESSVEQQLLDGKPYELPSMHSILERGFSLIGTRYRPGGSSTATGFDCS
                     GFIGYLFREEAGIKLPRSTREMINIPAPKVARHDLQPGDLIFFATSGRGRVNHAGIYI
                     GEGEFIHSSSTRSGGVRVDKLASSYWNSRYLMAKRVIDK"
     gene            complement(12947..21613)
                     /locus_tag="VIO83_07580"
     CDS             complement(12947..21613)
                     /locus_tag="VIO83_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY8331171.1"
                     /translation="MNIQRILTLLQLRRLETGFRLWRGSPRTFEYEPALRAELFSAEQ
                     MASHGAQLAREHRLSSRSASHSLLARLGDNEALLVRSCETLTTATLSTRRVMPAAEWL
                     LDNFYLIEEQIRIAQKHLPKGYSRELPRLANGPSAGLPRIYDIALEIISHGDGRVDSE
                     TLRRFVAAYQTVTPLTLGELWAIPIMLRLALIENLRRVSSRVMAKWNDRSLADDWADR
                     MTETAECDPKNIVLSVADMARSKPPMTSSFVAELVRRLQGQSSALALPLTWIEQHLAE
                     AGHTIEYMVQMDAQQQATDQVSIGNSIGSLRLLSATDWRDFVESLSLVEQVLRQDPPG
                     IYAAMDFATRDHYRHVVERLSRNSAHSEEAVARAALELAQANAGPDRAAAVAGHVGFY
                     LIDKGLPELEHRLAAHGTLEERYQRLLSRSPLRFYLGLATLLTLLFAAPFLGAAQASG
                     WHGLLLALLVIPALLTTSRLALGLVNWLVTLTVPPSLLPRLDLSEGIPAEARTLVVVP
                     TLIAREKDIEALVEGLEVRFLANRDAHLHFALLTDFMDAASEVLEQDAPLLALARERI
                     DELNRKYPDADHFFLFHRARRWNPAERVWMGHERKRGKLAQLNALLRGAGQEHFALIV
                     GNLAALQSVRYVITLDTDTQLPRDAARQFIGTMEHPLNRPVHDPQQRCITSGYAILQP
                     RVGISLPSTARSAYARLFGSEAGIDPYTRAVSDVYQDLFQQGSFIGKGIYAVDAFEQA
                     LTGCFPDNRILSHDLIEGCYARTGLLSDVQLFEEYPSRYSADSQRRHRWIRGDWQLLP
                     WLLPWVPTQCNGFEHNRLNALARWKIFDNLRRSLEPAALLTMLLAGWSGLAQPAGWTL
                     AILALLLIPPLLPSLQEIFQKPHDVPLDQHLAVVLRAAGQNLLRAALTLAWLPCEALC
                     CLDAITRTLWRMLVSHRRLLQWNPSCEVERTSVHDLPGLYRLMWASPLLAALVILALL
                     SSPVALAVATPLLLLWLASPAIAWWLGKPAERIAFAPTADELRFLRALSRRTWAFFDR
                     HVGPQDNWLPPDNLQEQPTAVIAHRTSPTNMGMALLAHLAAYDFGYLSAGRLLARLEQ
                     SLAVMNRLERHRNHFYNWYDTQSLQTLPPRYVSTVDSGNLAGLLLTLRPGLLELADDQ
                     VFDARQLAGLGDTLDVLRDALPAAALDSREMLALQAELDAARANPPPSLAAAADCLHG
                     LLTRAQALAEALNAAPESDVEFWLQALVEQCRDQLDELSRFLLPASASGAADDAVVAL
                     PTWRQLAQLEAGQWPAALREQVAEVQRAAAERIATATRLAELAGELAMMDFGFLYDTR
                     SNLFAIGYNVDEHLRDASYYDLLASEARLTNFVTIAQGQSPQQSWFTLGRLLTSNGGV
                     PVLLSWSGSMFEYLMPLLVMPNHEDTLLDQTCRAAVARQIAYGHQLGLPWGVSESGYN
                     TLDVNFNYQYRAFGVPGLGLQRGLGEDVVVAPYASALALMVAPQAACTNLQRLAEQGL
                     AGRYGLYEAVDYTAARLPRGHDAAVVQSFMAHHQGMSLLALASVLLGRPMQRRFESDP
                     QFRANALLLQERIPKTAAEYLHASGLATADVGTQVSAAKLRVFTDPDRRRPAVKLLSN
                     GRYHVLVSSGGGGYSRCRELALTRWHEDITCDNWGMFCYLRDVASGIFWSSAHQPTLR
                     RPQSYEAIFSEARAEFRVRERDFDTHTEIVVSPEDDIELRRIHVTNRARVRRTIELTS
                     YAEVVLASAISDALHPAFSNLFVQTELLEPLQAILCTRRPRSSEEVAPWMCHLLAAHE
                     VAIDSISYETDRRRFIGRGRSVVHPAAMDATPDGHGLLSNSAGSVLDPIVAIRCRITL
                     DPDQSATIDLMTGVNHSREGCLQLIDKYRDRHLADRVFDLAWTHSQVLRRQLNVSQVD
                     AQLYEQLATSVIYANAAFRADASVLTANQRNQSGLWGQGISGDLPIVLLQIHAAENIE
                     LVRQLVQAHAYWRLKGLAVDLVIWNEDRAGYRQHLQDLIMGLIASGIDASLIDRPAGV
                     FVRLAQQLSGEDRTLMQSVARLILSDDHGSLAEQVHRRRVEPPLPRFEPKLMRLGATA
                     AQPAAVPDAAADTLILRNPYGGFSADGDEYVLTLLPGQATPAPWVNVLANPDFGTVLS
                     ESGGAYTWSENAHEFRLTPWQSDPVGDVSGEALYLRDEMSGHYWSPTPLPRRGSGAYV
                     TRHGFGYSVFEHSEDGIHTELWVYVALDAPIKFSRLKIRNDSGRLRRLSATGYVEWVL
                     GDLRAKSAMHVVSEAEPLGALLAHNAYSLEFPGRVAFFDVDAPSRSVTADRSEFIGRN
                     GNLLAPAAMTQARLSGRTGAGLDPCAAIQVGFELGAGASHELVFRLGAEHDAAAANAL
                     LQRFRGSAAAAAVLDAVQAHWRETLGRVQVATPDPAVDVLVNGWLLYQVIVCRFWARS
                     GYYQSGGAFGFRDQLQDVMAMLHAAPHAARQHLLLCASRQFPEGDVQHWWHPPLGRGV
                     RTQCSDDYLWLALATSRYVQVTGDRSVLDEPAGYIEGRLLAAGEESYYDLPARAPLDE
                     TLYRHCVRAIEHGMARGVHGLPLIGAGDWNDGMNRVGEQGRGESVWLGFFLYEVLLNF
                     AETAREYGDDSFARHCEEQAATLQGNLEAHGWDGAWYRRAYFDDGTPLGSASNDECRI
                     DSIAQSWSVLSGAAPDERRRRAMDSLDRYLVRRDVGLIQLLEPPFDRSALDPGYIKGY
                     VPGVRENGGQYTHAAIWATMAFAALGDSARAWELLRMINPVSHGDSAAAIEVYKVEPY
                     VVAADVYGVFPHAGRGGWTWYTGSAGWMYRLMVESLLGLQVEGERLRIRPVLPAQWPG
                     FTLHYRFGASTYHIAVRQTAAAEGPAQFRLDGTLHSGEDIPLIDDGRDHQVEIDCRPR
                     GE"
     gene            complement(21844..22515)
                     /locus_tag="VIO83_07585"
     CDS             complement(21844..22515)
                     /locus_tag="VIO83_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY8331172.1"
                     /translation="MRDYPWLHEYCLNRFGSLAALEAHLPQPKSAAELRAVSDDRYLS
                     LLSLRIFRAGLKHSVVDGKWPAFEEVFFGFDPHKVVLMGAEHLERLMQDARLIRHLAK
                     LKSVPRNAQFVLDVAREHGSFGALLADWPASDITGLWRYLSKHGCQLGGLSAPRFLRM
                     AGKDTFIPTDDVVAALKAQGIVDKAPTSQRDLAAVQAAFNQWQEQSGRPLCQLSMMLA
                     CTVNH"
     gene            complement(22607..>23523)
                     /locus_tag="VIO83_07590"
     CDS             complement(22607..>23523)
                     /locus_tag="VIO83_07590"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY8331173.1"
                     /translation="IRAVLDGQDPRLLVVLGPCSLHDAESALDYAARLAALAPQVDDR
                     LLLVMRAYVEKPRTTIGWKGLVYDPHLDGSGDMAAGLALSRRLMLDILELGLPLASEL
                     LQPLAAGYFEDLLGWAAIGARTSESQIHREMVSGLDLPVGFKNGTDGSLGIACDAMRS
                     AAHAHRHFGIDALGHPALLETRGNPDTHLVLRGGHGGPNHDAGSVAAARVALERQGIP
                     ARIVVDCSHANSGKDPLRQPQVLEDVLDQRLAGDASLRGVMLESHLFDGCQPLSGALR
                     YGVSITDGCLGWAATEQSLLRAAERLRG"
BASE COUNT         3827 a         8047 c         8074 g         3575 t
ORIGIN      
        1 gcgcatccag gcggcctttc aggccggcca tggcctccag cgccgcgtgc tgcggcggct
       61 cgcccttctt ccaggcttcg gccaggccgt ccggggtcag gcgggtaaag ccggtgccga
      121 tatccagggc ggcctcgtcg ccggccgccc actcgcgcag cttggcgaac caaggctggt
      181 agtagcgcgc ctggatcttg cgcccgtcca ccgccttggc cgccacggcg gcatccagca
      241 gcgcctccag ttcgtcggcc caggccggcc acggcgcctt caactcggcc agttcgcggg
      301 cctgcgtttc cagcgcccgc tccagcagta cccccagcgc ctccgtcggc gccgcgtccg
      361 cctcgccgag cagcgggcgc aatttgtcct tgagcagctt gtccgggtgc agccagtgat
      421 tcatcaccca gcccagcgcc gcgccggcca gcggatagca ctgctggcgc cagtagtcgc
      481 gcaccacctc ggccatcagc tcgccgtggt cggtttccag ggtctgggtg aacaggctgc
      541 cgctgtcgaa ggcgtgctcg cgcagcatgc gctggcacca gccgtggatg gtggagaccg
      601 ccgcctcgtc catccactgc gccgcgatgt ccaggcggcg ggcgcaggcc ggccactgct
      661 cggccgggaa gtcgccgcgc agttggcgga gcagatcgtc gccctgctct tcgtcgcgga
      721 acaccttggc cgcctgcacc aggcgggcgc ggatgcggtc gcgcagctcg cgggtggcgg
      781 cgtcggtgaa ggtcaccacg aggatttccg gcggcagcag ttcgcgggcg aagccgtcgg
      841 cgccgccgtg gccgagcacc aggcgcaggt agagggcgga aatggtgaag gtcttgccgg
      901 taccggcgct ggcctcgatc agccggctgc cgtgcagcgg gaagcgcagc gccagcgggc
      961 gctcgttgtg cgggaactgg ctcatgcgcg ggcttcctcc ttgagttcgc ggatgtccgc
     1021 cttgagcagc ggctggtaga ggtcgtgggc ccactggtag aagtcgccgt cgacggtcag
     1081 cgccgcgaag tccgggtact gccgcgccag gctggcactc tgctcgacct cgccggtgcg
     1141 ctggtagcca ccctcgtagg cttcgctggc ggccttggct gccttgatct cgtcttcggc
     1201 ggacagccag gcgatggcgg tctgcagcgc caccggcagc ggtgcgttca ggccgtcgcg
     1261 ccagccttcc agccagcgct ggaggatctg ccgtgcctcg tcctgggcca ccggctggaa
     1321 ttcgaggcgg acatcctcgc cgaccagcag cgtggtcagc ggcacgccac aggccgccgc
     1381 ggcgacatga gccacccacg gccgtagcag gcggtgccac ttccagccct tgtccttgct
     1441 cttcagctcg ccgggctgca gctcgatgcg tgccagcgcc tcaccgccgc gactgcgcag
     1501 gccaccgagc cagccatcca gctcaacgcc ggcatgggcg aacggcaatt gccggggcga
     1561 gtccagccgg tcgggccaca gcgcgcacag cgcctggtag cgctgcatct gcgccggcag
     1621 cggctccagc acctgctcgc gcaggcgctc gccgaagccg gccaacggca gctggccgct
     1681 gcgctgcagg cgcagggcgg tcgcctgcag cgccggctcg acctgctcgg gcccctcctc
     1741 caacgccagg gtggcggcgc cgagcagctc ctggcgcagc tggtagagct gcaggccgtc
     1801 gaggccgaac ggctcctcgt cgagctgggc ctggacctcc tcgtcgaggt gcaccttgag
     1861 gcgctgggtg aagaagtgcc tgaccggatc gcgcaggaaa ttctgcaggg tctccagggc
     1921 cagcggggca tcggcctgga acggcggcag cgcgcccacc tccgctgcct gcgccgactg
     1981 ccggtgcagg tcggcccact cgcgggcata gctgaacagc tcgctgccgc cggcaaagta
     2041 gtcgcggctg aacggctgca gcgggtgctc ggtggtcagc gcgtgcagca ggtcgccacc
     2101 gccggttagc gtccagccgc tgccaagatg gtcacgcaac tggccgacca gcaccgaggc
     2161 cggccgctcg ctgttgtcgc ggatgctgcg gcccacccag ctgacataca gctgctcgcg
     2221 cgcggcgagc aaggcttcga gcagcaggta gcggtcgtcc tcgcggcgcg agcggtcacc
     2281 gggccggtag tcgccggcca tcaggtcgaa gtccagcggc gtcacgctgc gcgggtagtc
     2341 gccgtcattc atgccgagca ggcagaccat gcgaaacggg atggcgcgca tcggcatcag
     2401 ggtgcagaag ttcaccgcac cggcgaggaa gcgctggctg aggcggccct ggtccagccc
     2461 gccgagccag gcctcgcgca ccacggtgag cggcagcggc tcggtgagct cgacgccggc
     2521 gcacagctct agccagctat ccagcgcctc gaccagctgg ccgagcaggg tctcgtcgcg
     2581 ctcgttctgc gccacgaaga aatcggcgag cagtgcgcgc agggtcgtgc cccactcgct
     2641 tggggttgcc gaccgagcca gacgccgatg cagcgcatcg agggcatcca gcagcttgac
     2701 cagcgggccg atcagcgcgg cgtccagccc gccgatctcg tcatagggtt cgatgccggc
     2761 gcaggccgcg ccgctgccca ccgcgtagcc gagcagcatg cggcgcaggc cgaagcgcca
     2821 ggtgttctgc tccaggccgt ccggcaggcc cagcgaggca cggcgctcgg cgtccaggcc
     2881 ccagcggatg ccggcgcctt cgatccagcg gtgcagggtc ggcagctcgc tctcgtcgat
     2941 ggcgaagcgc gcacgcagcg ccggcacgtc gagcaggtcg agcacctcgc tgacggtcag
     3001 ccggccttcc ggcagtttca gcaggtgttc gacggcgatc agcagcggct cgcagccgcg
     3061 ctggccctgg tcggccaggg tgaaggggat gtagcgagcg tcgtcctggg cgtactggcc
     3121 aaacaccgcc tggatgtgcg gggcgtaggc gttgacgtcc ggcaccatga cgatgatctc
     3181 gcgcgggcgc aggctcgggt cggcggaaaa gcgcgccagc agctggtcgt ggaggatctc
     3241 cacctcgcgc tgggcgctgt gggcgatatg gaagcgcagc gactggtcct tcgccgggtc
     3301 gagttcaggc caggtatcgc gggtttccgc cagcgggcgc agctcgagga tgtcgtcctg
     3361 cagctggccg agcaggtgcg cggtgtcgcc ctcgccgaac aggtcgatgc gctgattgat
     3421 cgcggcgaaa cgcgcgcggt agtgctcggg gtcgtcgtgc tggtcgagca ggttgatgta
     3481 gtcgcggccc tgcttgcccc aggcggccag cagcggatgg gcgtgctggt gcagctcgtc
     3541 ggcggacagg ccgggcgtca tgccgccctt gtgctgctgg cggcggtact ggtggcgcag
     3601 caggtcctgg tcaggaacga tgtcgcccca atggtgctgg caggggttgt gcacgcacag
     3661 cagcacctgg ctgaagcgcg ccagggcggc cagcgcctcg accatctgcg ccggcagcga
     3721 ggagatgccg aacacgatca cgcggcgcgg caggccgcgc ggcctctcgt cgacgttgcg
     3781 caggtgcgcg acgaagcgcg gatgcacgcc ggcgcggctc tcgcccagct gctcgcggcc
     3841 gacatcctcc agcagcgcgc gccacagcgc cggctgccag cgcaccgcct cgtccagcgc
     3901 cttggcgcca ttgcgcgagg tgcgcagctg atccagaccg gcagcccagt cggccagcca
     3961 gtcggcgcgg tacacctggt actggtcgaa caggtcggcc aggcgctcgg ccagctggtg
     4021 gcgcttgcgc aggtcgctgt cgtcggcgag gaagcggcgc agcggagcga acgcttcacc
     4081 ggccagcaga ttgggcagca gacgcatcag ccgccaggtc agcggcgcct tgtccagcgg
     4141 cgactccagt ggaatgctct cgctgcccag cacggcgcgg taggtctgcc agaggaagcg
     4201 cgccggcagc tcgacctgga tggccgcagc gatgccgcaa ctgtcctggg cggccagcgc
     4261 cagcttcagc cactgagcga tgccgttgct ctgcaccagc accacctcgt tctccagcgg
     4321 cgccagcgga tgcaggcgca tccactgcac cgccagttcg cgcagctcct ccaggcggtt
     4381 gccgtgcacc accatcagcc ccggcgtcag ctcgttgccc tgcatgtctc gctcccttca
     4441 ccgacagagc gcgcattgtg ccacccgggt gcgacaaaga gtgtcgcggg ggaacgggcg
     4501 gctgctaacc tgcggcgatg aaacagccga tcagcggata ccaccgcgac gacgaaaacc
     4561 actgggttgc cgagctggcc tgcggccaca accagcacgt gcggcacaac ccgccgtggg
     4621 tcagccggcc gtgggtgacg acagctgcgg ggcgagagcg gatgctgggt tacgaattac
     4681 agtgccggaa atgcgatgca ggggcgccgc cggatcggcc ccaggcgccg gatgggccag
     4741 atagcgcacc gtgacccgtt cgcggtttgc cggtgcgcga ccgggcgctc gcgatggtgg
     4801 gttacgccgc tgcgcggccg acccacccga cgaaaagcct cgggctcagc gcccgatcag
     4861 ctatccgaat cgcgcgaggc gaggatcgcc gccacccgct gcggcttgca gttgaggtag
     4921 gcggaagact gcatccactt gcggtcggga taccaggaga acatgaactg cccccccttg
     4981 agattgtcta tcaccatacg agccacttcc ggacgcaccg ccgggcaacc gtggctgcgg
     5041 ccgatgcggc catggcgctt gacccaggcc gggtcgacgt agtcggcggg atggatgacg
     5101 atggcgcgct cgcgggcacg gtcgttgatg cccggctcca ggccgtccat gcgcaacgag
     5161 tagccgtgtt ggccgtgata ggtttccgag gtgcggaaca ggccgatgct cgactgatgg
     5221 ctgccttcgc gattggagaa actggtggcg aagttttcgc cggagccctg cccgtgggcg
     5281 accagctcgc gcagcagcag ggtcttgcgc ttgagatcga agacccacag acggcgctcc
     5341 gacgagggca gcgagaagtc gatgaccgcc agacggtcgg ccggcgcgga gccgttgttg
     5401 accgcgcact gcatggccgc cagggcgtgg cgcagcactt cgtggttcag ggtcggcgcc
     5461 gccttggcca gactgtcgat caacggcgca gcgctggggg agcttgccag aagtggagaa
     5521 gagaaagccg caagacttgc agccagccag agcgagcgaa tcagcctggt catggagaaa
     5581 ggtgtcctct gtatggtgta ccgacagagc acgggaaact gcggcgccaa cctcgctggc
     5641 gcaccgcccc acgcaccgcc cgtccgttac atgacgccct gtcgttgtta ctgtcgtcct
     5701 gcttatgatc aacggcgtac atcagcatct agctgcgtta ccgctcgtta ttaaggcatt
     5761 ctagcaagca aatgatggtc gggagctgag cgtttgtcta aaaaatacac acccatgctg
     5821 gcaatgctgc tggtgagctg ccagctggcc gtcgccggag cagtcgctgg cgagtctgac
     5881 aacgcgctgc aagccgaatt gcaacagggc cagctgagct gtgccagtcg ccccttgccg
     5941 ctcgcggcca gcgcgcggga aacgctgctc gacttctacc ggcaacaggg ctccgagcgc
     6001 gtgtggaacc gtgctcgtct cgccagcctg ctgacccagc tggagcgcct ggccgacgac
     6061 ggcctgtcgc cggaggccta tcacctggcc gatctgcgca ggctcgagcg catctccgcc
     6121 agcgacgccc agcgcacggc ctgccgcgag gtgctggcca gccatgccta cttgcaggcg
     6181 ctgcaggacc tgagctgggg ccggctcgat ccggcccagg tggagccgat ctggcactcc
     6241 cagcacacgc cggcacccag ccggccgagc ggagctccgg cttttgcgac cagcggactg
     6301 ggcgacctgc ccgccacctt cgagcgggcg cgtccgcagt tcaagcccta tcgcaacctg
     6361 cgcaacgcct atgcccgcct ccgccagcag ccgttgcccg catggccgcg gatcgccgac
     6421 ggcgcgctgc tgcgcccggg caagcgcgat gcccgtgtgc cgttgctgga gcagcgcctg
     6481 gcccgtgcag gctatctgac cagaacggcg ggccgcccgg ctggcgcaga cagcgacctg
     6541 tacgatccgt tgctggtgga cgccgtcgaa accttccagc gcgaccatca gctgcaaccc
     6601 gacggcgtcg tcggcccggc gaccctggcc gaactgaaca tctcggccgc cgcccgcagg
     6661 gaccaggtgc gcgtcaacct cgagcgcctg cgctggctgg cccgggaaat ggagccgagc
     6721 atggtgctgg tggatctcgc cggcacccgg gtgtcctact accgcgaccg ccagctgctg
     6781 tggcaggcac gtacccaggt gggccgggcc gagcgcccct cgccactgtt gaagtcgcgc
     6841 atcacccacc tgaccctgaa cccgacctgg acggtgccac cgaccatctt ccgcaaggac
     6901 aagctgccgg aaatccagcg caacatcggc tatctggcgg aaaaccgcat gcgcgtgctg
     6961 gataacgccg gcaacgagct gaatccgcac gccgtcgact ggtacaaccc ccagggcatc
     7021 ctgctgcgcc aggacgccgg cccgacaagc gcgctgggcg tgctcgccat ccgctttccc
     7081 aacccgttct cggtgtatct gcacgacacc cccagccagc agctgttcaa caagttgccg
     7141 cgggtgttca gttcgggctg cgtgcgggtc gagaacgtcc tgcagctgct cgaccacctg
     7201 ctggccgacg ccagcccggc cgagcgcgag cgcatcgccg cgatccacgc cagcggcaag
     7261 accaagaacg tcaacctgcc ccacgcggtg accatcctgc tggcctactg gacggcggag
     7321 gccgacgacg ctggccgact gcagttccgc ccggacatct accagcacga cgcgccgatc
     7381 ctcgccgcgc tggaggcggc cgagcgctga ctggcggccc ttgcgcgcgc cccgggaggc
     7441 gcctcgcctg cgcggactgc tctacgctga aagccaatag cccgggcggc gaggcctcgg
     7501 gcgcaccgga aacggagttc atcatggatc tcgaacagta cctgcgcgcc ctgcccaagg
     7561 cggagctgca cctgcacatc gaaggcaccc tggagccgga gctgatgttc gcgctggccg
     7621 aacgcaatgg catcgccctg ccctggcaca gtgtcgagga aacccgtgcc gcctatgcct
     7681 tcagcgatct gcagagcttt ctcgacctgt actacgccgg cgctgccgcg ttgcttcgcg
     7741 aggaggactt cttcgacctg gccatggcct acttcgagcg cgcccacgcc gatggcgtgg
     7801 tgcatgccga gctgttcttc gacccgcaga cccacaccgc ccgcggggtg gccttcgcca
     7861 ccgtgttcga cggcctggag cgcgcctgca gcgcggcccg tgagcgctat aacatcagct
     7921 cgcgactgat tctctgcttc ctgcgccacc tcagcgagga ggacggcttc gccaccctcg
     7981 agcaggccct gccgcacctc gcccgtatcc acggcgtcgg cctcgactcc tcggagcagg
     8041 gccatccgcc gagcaagttc gtccgcctgt tcgcccgctg ccgcgcgctg ggcctgcaca
     8101 ttgtcgccca tgccggcgag gaaggcccgc cggcctacat cgtcgaggcg ctggacctgc
     8161 tcaaggccga gcgcatcgac cacggcgtgc gtgccgccga ggacccggca ctgctccagc
     8221 gcctggcacg cgagcaggtg ccgctcaccg tctgcccgct gtccaacgtc aagttgtgcg
     8281 tcttccccaa cctgcgccag cacaacctca agaccctcct cgatgcgggc ctcaaggtga
     8341 cgatcaactc cgacgatccg gcgtatttcg gcggctatgt actggagaac taccggcaga
     8401 ccgcgctggc gctgcagctg tcgcgtgacg aactcaagcg catcgcgcgc aacagcctgg
     8461 aagcggcctt tgtcgacgcc gtcgagcgcg cgccctggct ggcacggctg gaggcactgg
     8521 cggactgaac gaggcagccg cgccgctgcg gatctacgct gtaagtcgtc atccggatag
     8581 ctatcgagag gccaaccatg aacagcaagc ccgccggcga acgcttccgg ctgtattcgg
     8641 cgcagcagct cgatggtgtg atcgagcaga tggcgcgcca ggccgcggcg ctgttcgtcg
     8701 ggcgccgcga ggtggtgctg gtaggcatcc tgcgccgcgg cgaaccgttg gcgcagcgta
     8761 tgcagagtgc gctggtggag cattgcggcc tgccgcgctg gccgctgtac ccgctgcacc
     8821 tgaagcgcta tgccgacgac ctcagcctgc tgcatgcgga aacggcgctg accgagaacc
     8881 ccgaactggc cgccttggat ctgtcgcgca ccagcgtgct gctggtcgac gacgtgcttt
     8941 acgaagggca ctcgctgctg cgggccagcg cctggctggc gcaactgggc gcgatggaga
     9001 cgcgcacggc ggtgctggtc gaccgttgcg tgagccgcca gccggtacgc gccgacatca
     9061 ccgggctgcg cctgcaggtg gcccccaccg acatcatcga atgcaacgtg ccaccctatg
     9121 agcgcgaatt cggcatcgag gtgctgcgcc gcgccctgaa ttgactccgc gcgggggcat
     9181 ccgcagcact tccgaccatc gcctgggctg gcaagcgcag gactcgcccg tataatgcgc
     9241 cggttcgcag gagagtgagc gttcgccgcg gccgccaagg ccagccggac gctccgccga
     9301 aggcgcaaac tcccataatc gctcaggctc aggaactgcg gacgcccatt ttcgccacat
     9361 ctggagagcg gttgctgcgg gcaacccacc gaaggggcaa gcagccgacg gctgcgtaaa
     9421 ctctcaggta cacaggacag agggagaggc gctgcggact taaggagtcc tctcttgctc
     9481 atctacatct atctcatcgc gatcacggcc gaggccatgt ccggtgcgct ggccgccggc
     9541 cggcgcaaca tggacatgtt cggcgtgggc ctgatcgcct tcgtcaccgc actcggcggc
     9601 ggcacggtgc gcgacatcct gctcggccac tttccggtgg gctggaccca gcatccgagc
     9661 tatatctacc tgaccatcgg cgccggcctg ttcaccatgc tggtcgcccg cttcatgcac
     9721 cacctgcaca agatcttcct ggtgctcgac gccatgggcc tgatcgcctt caccgtgatc
     9781 ggctgcaacg tggcgctgga gctcaactac tcgaccccgg tggtgatcat ggccggcatg
     9841 atcaccggcg tgttcggcgg catcctgcgc gacatcctct gccagcgcgt accggtggtc
     9901 ctgcgccacg agctgtatgc cagcgtctcc atgctggtgg ccatgctcta tctgctcctg
     9961 aacaagctgg gcatcaacca cgacatcacc ctgctggcct ccttcggctt cggcctggcc
    10021 ttgcgcctgg cggcgatccg ctggtcgtgg tcgctgccgg tgttttccta ccagcctggc
    10081 cgctgggaat agtcagcgcg cccaggcctg gcgcagcaac gcctgcacct cctcgtcgct
    10141 ggtctggccg aagtcgctgt accactcgcc gatggaggac agggcgtccg gcaccagcgg
    10201 gcagaccact tcgtccacca gcgggcgcag catggcgatg gcgtcggtgg aagccaccgg
    10261 cgccaccagc acgatgcgcg ccggtgcctg ctggcgcacc gcctgcaccg ccgcgcgcat
    10321 ggtcgaaccg gtcgccaggc catcgtcgac cagcagcacc tggcagccct gcagctcagg
    10381 atacggacga ttgccgcggt agaggtggtc gcgccgctca agctcgctct tctgacgctc
    10441 ggtcaccctg tcgatagtcc cggcgtcgac acgcccgtag agcagcgcct cctcgttgag
    10501 cacccgcacc ccgccgctgg cgatcgcgcc catggcgtac tcctcgtgcc cgggcatgcc
    10561 gagcttgcgc accagcatca ggtcgagcgg cagcgccaac tcacgagcga tctcccacgc
    10621 caccggcact ccgccgcgcg gcagggccaa gacgagggtg tcgggctggt tgcggtaggc
    10681 atcgagcagg cgggccaggg tcttgccggc cgccacccgg tcgggaatcg gcacttcgat
    10741 ctgcgccttg cgcatcatgc aaacctccgg ctggctgcag gtcgagcagc ctgttcctgc
    10801 aagcctatac cgggccgccg ggtctgcaag ccagcaacag cctgacgccg agtcgctatc
    10861 agaatccccc tgcaaacagc gaatgcaact aagggtgcag ggacaggctt gaccataatc
    10921 aaacagtccc ccggtgcgct tttcaagact ggagaggaca tgtggccgac acgggcatcg
    10981 tggggccggc agaccaacct ttcagcggaa gtcagttatg gacaagcata agcagggcca
    11041 caaagacaca ctgctccagc ctgggcacca tgatcgagac aaggagcacc agcatcttgc
    11101 cggcaccgcc cactgcaccg gctgcggcgc ctatttccat gccggtcgct ggtcgtggca
    11161 gcggcaggcc ggcagccacc ccgaggagat cacctgcccg gcctgccggc gcatcgccga
    11221 aaacgacgcg gccggcaacg tgaccctgtc aggcggattc gtacaggcgc accgcgacga
    11281 cctcctcaac ctgatccgca acaccgagga aggcgagagc aaggagcatc cgctggagcg
    11341 cctgatcggc atcagcgagg aagacgacaa ggtcatggtc ggcaccaccg gcacgcatct
    11401 ggccaaccgc atcggccacg ccctggaggc cgcctacgat ggggaagtga gctacaccta
    11461 caccgaaaac gaaacccacc tggacgtcaa ctggcagcgc gactgagcac tgccaggtgg
    11521 gctcaggggt tgcgcgccag gcgctcccct gccagcacgc gccgggcatc gaggtagcgt
    11581 tgttgccagt agctgttgtt gagactgtcg agacgcaccg tgccgccggc actgggagca
    11641 tgcacgaagc ggtcttcgcc aacgtagata ccggcatgac tgacctggcg gccaccattg
    11701 gtggcgaaga acaccagatc gcccgactgc aggtgctgac gcgccacttg gggcgcgcgc
    11761 agggcgctca tctcgcgggt ggtacgcggc aactgcaccc cggcggcatc gcggtacaca
    11821 tagccgatca ggccgctgca gtcgaaaccg ctgtcaggcg tgttgccgcc ccagcgatag
    11881 ggggtgccga ccaggccgat ggcgcgaaac agcacatctt cggcaggtcc gtaagcggcc
    11941 ggcgcctggc tgacaggtgt ctggggggga agcggagcct gcgaggagca gcctgcgagg
    12001 agcgcgacga gaaggataca ggctagacgg attggcgctg tcatgtggca tatccctgcg
    12061 gcaaacgcct gcgactgtgc cgcgcatgcg agcgcggcac cctttcatcg agcggactcc
    12121 cggcgcgccc cacaggggta ttgccgggtc ttgcgcttat ttgtcgataa cgcgcttggc
    12181 catcaggtaa cgactgttcc agtagctgct ggccagcttg tccacccgca ctccaccgct
    12241 acgggtgctg gaagagtgga tgaactcgcc ctcaccaata tagatgcccg cgtgatttac
    12301 gcgaccacga ccgctggtgg cgaagaaaat caggtcgccg ggctgcaggt cgtgacgggc
    12361 gactttcggc gccggaatgt tgatcatctc gcgggtcgag cgcggcagct tgatgcccgc
    12421 ctcttcgcgg aacagatagc cgatgaaacc gctgcaatcg aagccggttg cggtcgagct
    12481 gccacccgga cggtagcgcg tcccgatcag ggagaagccg cgctccagga tggagtgcat
    12541 gctcggcagc tcatagggct tgccgtcgag caactgctgc tcgacgctgc tctcttccgc
    12601 cttggcggcg gctgcggcgt ccagctcgaa ggacagctgc tggctcggac tgctgaatga
    12661 ctgctgtggc tgttgtgcct ggtgcgaagt actggcgcaa gcagcgagaa aaagggccag
    12721 ggtcagtggc acgaggggtg cgaagcgttt gagcatgggc acgaccgtgt cgttataaag
    12781 gttgctgaaa tttgatgaga tcggactatg ccggctatga ggtcgatttg caaattttat
    12841 agcgctgagt gtgactttac gccgcaaagg tgccgtctgc gcgactgaac gccgcaggca
    12901 gcgggctttc agagctaaaa cggcgcggcc gccaacggct cattggctat tcgccgcgcg
    12961 gccggcagtc gatttcgacc tgatgatcgc gaccatcgtc gatcagcgga atgtcctcgc
    13021 cgctgtgcag cgtgccgtcc aggcggaact gtgccggccc ctcggcggcc gcggtctgcc
    13081 gcacggcgat gtggtaggtg cttgcgccaa agcggtaatg cagggtgaag ccgggccatt
    13141 gtgcggggag cacagggcgg atgcgcaggc gctcgccttc cacctgcagg cccagcaggg
    13201 attccaccat cagccggtac atccagccgg ccgagccggt gtaccaggtc cagccgccgc
    13261 gtcccgcatg ggggaacacc ccgtacacgt cggcggccac cacgtatggc tcgaccttgt
    13321 agacctcgat cgccgcggcg ctgtcgccat ggctgaccgg gttgatcatg cgcagcagct
    13381 cccaggcccg ggcgctgtcg cccagcgcag cgaacgccat ggtcgcccag atggccgcat
    13441 gggtgtactg gccgccgttc tcgcgcacgc cgggcacgta gcccttgatg tagccggggt
    13501 cgagcgccga gcggtcgaaa ggtggctcga gcaactggat cagcccgaca tcgcggcgca
    13561 ccagatagcg gtcgagcgaa tccatcgccc ggcgccgtcg ttcatccggt gcggcaccgg
    13621 agagcaccga ccagctctgc gcgatcgaat cgatccggca ttcgtcgttg ctcgccgagc
    13681 ccagcggcgt gccgtcgtcg aaataggcgc ggcggtacca ggcgccatcc cagccatgcg
    13741 cctcgaggtt gccctgcagg gtggcggcct gctcctcgca atgccgggcg aaagaatcgt
    13801 cgccatactc acgcgcggtc tcggcaaagt tcaacagcac ctcgtagagg aagaagccca
    13861 gccacacgct ttcgccgcgg ccctgctcac cgacccggtt catgccgtcg ttccagtcgc
    13921 cggcgccgat cagcggcagg ccatggacgc cgcgggccat gccgtgctcg atggcccgca
    13981 cgcagtgccg atacagcgtt tcgtccagcg gcgcgcgcgc cggcaggtcg tagtaggact
    14041 cttcgcccgc ggccagcagg cggccctcga tatagccggc cggctcgtcg agcacgctgc
    14101 gatctccggt gacctgtaca taacggctgg tggccagggc cagccacagg tagtcgtcgg
    14161 agcactgggt gcgcacgccg cgccccagcg gcggatgcca ccagtgctgt acatcgccct
    14221 cggggaactg gcgcgaggcg cacagcagca ggtgctggcg cgcggcgtgc ggggcggcgt
    14281 ggagcatcgc catgacgtcc tgcagctggt cgcggaagcc gaacgccccg ccggactggt
    14341 aataaccgct gcgcgcccag aagcgacaga cgatgacctg gtacagcagc cagccgttga
    14401 ccagcacgtc gaccgccgga tcgggggttg ccacctgcac ccggcccagc gtttcgcgcc
    14461 agtgcgcctg cacggcgtcg agcaccgcgg ccgccgccgc actgccacgg aagcgctgca
    14521 gcagggcgtt cgccgccgcg gcgtcgtgtt ccgcgcccag gcggaacacc agttcgtggc
    14581 tggccccggc acccagctcg aagccgacct ggatcgccgc gcaggggtcc agtccggcgc
    14641 cggtccgccc ggacagccgc gcctgggtca ttgctgccgg tgccagcagg ttgccgttgc
    14701 ggccgatgaa ctcgctgcgg tcggcggtga cgctgcgact cggcgcgtcg acgtcgaaga
    14761 acgccacccg gccgggaaac tcgagcgaat aggcattgtg cgccagcagg gcgccgagcg
    14821 gctcggcttc gctgaccacg tgcatcgccg acttggcgcg caagtcgccc agcacccact
    14881 cgacatagcc ggtggcggac agccggcgca ggcgccccga gtcgttgcgg atcttgagcc
    14941 gggaaaactt gatcggcgca tccagcgcca cgtacaccca cagctcggtg tggatgccgt
    15001 cctcgctgtg ctcgaacacg ctgtagccga agccgtggcg ggtcacgtac gcgccgctgc
    15061 cgcggcgcgg cagcggggtc ggcgaccagt agtggccact catctcgtcg cgcaggtaca
    15121 acgcctcgcc gctgacatcg ccgaccggat cgctctgcca gggcgtcagg cggaactcgt
    15181 gggcgttctc gctccaggta taggcgccgc cgctctcgga gaggacggtg ccgaagtcgg
    15241 gattggccag cacgttgacc cagggcgccg gggtcgcctg ccccggcagc agggtcaaca
    15301 catattcgtc gccgtccgcg ctgaagccgc cgtagggatt gcgcaggatc agcgtgtccg
    15361 ccgcggcatc gggcactgcg gcgggctgcg cggccgtggc gccgaggcgc atcagcttcg
    15421 gctcgaagcg cggcagcggc ggctccacgc ggcgccggtg cacctgctcg gccaggctgc
    15481 catggtcgtc gctgaggatc agccgggcga ccgactgcat cagggtgcgg tcctcgcccg
    15541 acagctgctg ggccaggcgc acgaagaccc cggccggacg gtcgatcagg ctggcatcga
    15601 tgcccgaggc gatcaggccc atgatcaggt cctgcaggtg ttgccggtag ccggcacggt
    15661 cctcgttcca gatcaccagg tccaccgcca ggcctttcag gcgccagtag gcatgcgcct
    15721 gcaccagctg gcggaccagc tcgatattct ccgccgcgtg gatttgcagc agcacgatcg
    15781 gcagatcgcc ggagatgccc tgcccccaca ggccggactg attgcgctga ttggcggtca
    15841 gcacgctggc atcggccctg aatgcagcat tcgcgtagat gaccgaggta gccagctgct
    15901 cgtacagctg cgcatcgacc tgcgagacat tgagctggcg gcgcaggacc tggctatggg
    15961 tccacgccag gtcgaacacg cggtcggcca gatggcggtc gcggtatttg tcgatcagct
    16021 gcaggcagcc ctcgcgactg tggttgacgc cagtcatcag gtcgatggtc gccgactggt
    16081 cgggatcgag ggtgatgcgg cagcggatcg ccacgatcgg atcgagcacc gagccggcgc
    16141 tgttggagag caggccgtgc ccgtccggcg tggcgtccat cgccgccgga tggacgacgc
    16201 tgcggccgcg gccgataaag cgccggcggt cggtttcgta ggagatcgag tcgatggcga
    16261 cctcgtgcgc cgccagcagg tggcacatcc agggcgccac ttcctcgctg gagcgcggcc
    16321 gacgggtaca gaggatcgcc tgcagcggct ccagcagctc ggtctgcacg aacaggttgc
    16381 tgaacgccgg atgcagcgca tcactgatcg ccgaggccag caccacttcg gcatagctgg
    16441 tgagctcgat ggtgcggcgg acgcgggcac ggttggtgac gtggatacgg cgcagctcga
    16501 tgtcgtcctc cggcgacacc acgatctcgg tatgggtgtc gaagtcgcgc tcgcgcacgc
    16561 gaaactcggc ccgcgcctcg gagaagatcg cctcgtaact ttgcgggcgc cgcagcgtcg
    16621 gctggtgggc gctggaccag aagatgccac tggcgacatc gcgcaggtag cagaacatgc
    16681 cccagttgtc gcaggtgatg tcctcgtgcc agcgggtcag cgccagctcg cggcagcggc
    16741 tgtagccgcc accgccgctg ctcaccagca cgtgatagcg cccgttggac agcagcttga
    16801 cggcaggccg gcggcggtcg ggatcggtga agacccgcag cttggcggcg ctcacctgcg
    16861 tccccacgtc ggcggtggca aggccggagg catgcaggta ctcggcggcc gttttcggga
    16921 tgcgttcctg cagcagcaag gcgttggcgc gaaattgcgg gtcggattcg aagcggcgct
    16981 gcatcggccg gccgagcagc acgctggcca gcgccagcag actcatgccc tggtggtgcg
    17041 ccatgaagga ctggaccact gccgcgtcgt gcccgcgcgg caggcgcgcc gcggtgtagt
    17101 cgaccgcctc gtacaggcca tagcgtccgg ccagaccttg ctcagcgagg cgctgcaggt
    17161 ttgtgcaggc cgcctgtggg gccaccatca acgccagtgc cgaggcatac ggcgccacca
    17221 ccacgtcctc gccgagcccg cgctggagcc ccagccccgg caccccgaag gcgcggtact
    17281 gatagttgaa gttgacatcc agggtgttgt agcccgactc ggagacgccc cacggcagcc
    17341 ccagctggtg gccgtaggcg atctgccggg ccaccgccgc gcggcaggtc tggtcgagca
    17401 gggtgtcctc gtggttcggc atcaccagca gcggcatcag atactcgaac atcgacccgg
    17461 accaggacag caacaccggc acgccgccgt tgctggtcag cagccggccg agggtgaacc
    17521 agctctgctg gggcgactgt ccctgggcga tggtcacgaa gttggtcaga cgcgcctccg
    17581 aggccagcag gtcgtagtag ctcgcatcgc gcaggtgctc gtcgacgttg tagccgatgg
    17641 cgaacaggtt gctgcgcgtg tcgtagagaa agccgaaatc catcatcgcc agctcgccgg
    17701 ccagctcggc cagccgcgtg gcggtggcga tgcgctcggc ggcggcgcgc tgcacttcgg
    17761 caacctgttc gcgcaacgcc gcgggccact ggccggcctc cagctgcgcc agctggcgcc
    17821 aggtgggcag cgccaccaca gcgtcgtctg ccgcgccgct cgcgctcgcc ggcagcagga
    17881 aacggctcaa ctcatccagt tggtcgcggc attgctcgac cagcgcctgc agccagaact
    17941 ccacgtcgct ctccggtgcg gcattgagcg cctcggccag cgcttgcgcg cgagtgagca
    18001 gcccatgaag gcagtccgcc gcggccgcca gcgagggcgg cgggttggct cgcgccgcgt
    18061 ccagctcggc ctgcaacgcg agcatctcgc ggctgtcgag cgcggcggcc gggagcgcgt
    18121 cgcgcagcac atcgagggta tcgcccaggc ccgccagctg gcgggcgtcg aacacctgat
    18181 cgtcggccag ctccagcagt ccggggcgca gggtcagcag cagcccggcc aggttgccgc
    18241 tgtccaccgt cgacacgtag cgcggcggca gggtttgcaa cgactgggtg tcgtaccagt
    18301 tgtagaagtg gttgcgatgg cgttccagcc ggttcatgac ggcgagcgac tgctccagac
    18361 gcgccagcag tcgcccagcg ctcaggtagc cgaagtcata ggcagccagg tgcgccagca
    18421 gggccatgcc catgttggtc ggcgaggtgc ggtgggcgat gacggcggta ggctgctcct
    18481 gcagattgtc cggcggcagc cagttgtcct gcgggccgac atggcggtcg aagaaagccc
    18541 aggtccgccg cgacaaggcg cgcagaaaac gtagctcgtc ggccgtcggg gcgaatgcta
    18601 tgcgctcggc cggcttgccc agccaccagg cgatcgccgg cgaggccagc cacagcaaca
    18661 gtaacggggt ggcgaccgcc agtgcgacgg ggctggacag caaggccagg atcaccagag
    18721 cggccagcag cggactggcc cacatcagcc gatagagtcc cggcagatcg tggacgctgg
    18781 tgcgctcgac ttcgcaggag ggattccact gcaacaaccg gcgatgactg accagcatgc
    18841 gccacagcgt gcgcgtgatc gcatccaggc agcacagcgc ctcgcacggc agccaggcca
    18901 gggtcagcgc cgcgcgcagg agattctgcc ccgcggcgcg caggaccacc gccaggtgct
    18961 ggtcgagcgg cacgtcgtgg ggcttctgga agatctcctg caaggatggc aacagcggcg
    19021 ggatcagcag cagggcgagg atcgccaggg tccagccggc cggctgcgcc aggccggacc
    19081 agcctgccag cagcatcgtc agcaaggccg ccggctccag gctgcggcgc aggttgtcga
    19141 aaatcttcca gcgcgccagc gcgttcaagc gattgtgctc gaagccgttg cactgcgtcg
    19201 gcacccacgg cagcagccac ggcaacagct gccagtcgcc gcgaatccag cgatgccggc
    19261 gctggctgtc ggcgctgtag cgcgagggat actcctcgaa caactgcaca tcgctgagca
    19321 gccccgtgcg ggcgtagcag ccctcgatca ggtcgtgact gaggatgcgg ttgtcgggga
    19381 aacacccggt gagggcctgc tcgaaggcat ccaccgcata gatgcccttg ccgatgaacg
    19441 agccctgctg gaacaggtcc tgatagacgt cggagacggc gcgggtgtag ggatcgatcc
    19501 ccgcctcgct gccgaacagc cgggcatagg cggagcgtgc cgtgctcggc aggctgatgc
    19561 cgacccgcgg ttgcaggatc gcgtagccgg aggtgatgca gcgctgctgc ggatcgtgga
    19621 cgggacggtt gagcggatgc tccatggtgc cgatgaactg gcgcgccgcg tcgcgcggca
    19681 gctgggtgtc ggtgtccagg gtgatgacgt agcgcaccga ctgcaaggcc gcgagattgc
    19741 cgacgatcag cgcaaagtgc tcctgccccg cgccgcgcaa cagcgcattg agctgggcga
    19801 gcttgccgcg cttgcgctcg tggcccatcc agactcgctc ggccggattc cagcgccggg
    19861 cgcggtggaa caggaagaag tgatcggcgt cgggatattt gcggttcaac tcgtcgatgc
    19921 gttcgcgggc cagcgccagc agtggcgcat cctgctccag cacctcgctg gccgcatcca
    19981 tgaagtcggt cagcagggcg aaatgcagat gggcgtcgcg gttggccaga aagcgcacct
    20041 ccaggccctc gaccagcgcc tcgatatcct tctcccgggc gatcagcgtc ggcaccacca
    20101 ccagcgtgcg cgcctcggcg gggatgccct cgctcaagtc caggcgcggc agcaggctcg
    20161 gcggcacggt cagcgtcacc agccagttga ccaggccgag cgccagccgg ctggtggtca
    20221 gcagcgcggg aatcaccagc aatgccagca acagtccatg ccaaccgctg gcctgtgccg
    20281 cccccaggaa cggcgcggcg aacagcaggg tcagcagcgt ggccaggccg aggtagaagc
    20341 gcagcggtga gcggctcagc agacgctggt agcgttcttc cagcgtgcca tgggcggcga
    20401 ggcggtgctc cagctcgggc agccccttgt cgatcaggta gaagccgacg tgaccggcaa
    20461 cggccgcagc gcgatccggc ccggcgttgg cctgcgccag ctcgagcgcc gcgcgcgcca
    20521 ccgcttcctc cgagtgcgca ctgtttctgg acaggcgttc gacgacatgc cggtaatggt
    20581 cgcgcgtcgc gaaatccatg gcggcataga tccccggcgg atcctggcgc agcacctgct
    20641 cgaccaggct caggctctcg acgaaatcgc gccagtcggt cgccgacagc aggcgcaggc
    20701 tgccgatgct gttgccgatg gacacctggt cggtcgcctg ctgctgggca tccatctgca
    20761 ccatgtactc gatggtgtgc cccgcctcgg ccaggtgctg ttcgatccag gtcagcggca
    20821 gggccagcgc cgaactctgc ccctgcagcc ggcgcaccag ttcggcgacg aacgaactgg
    20881 tcatcggcgg cttcgagcgc gccatgtcgg cgacgctgag cacgatgttc ttcgggtcgc
    20941 actcggcggt ttcggtcatc cggtcggccc agtcgtcagc caggctgcga tcgttccatt
    21001 tggccatgac ccgcgaggac acccggcgca aattctcgat caaggccagg cgcagcatga
    21061 tcgggatcgc ccacagctcg ccgagggtga gcggggtgac cgtctggtag gcggcgacga
    21121 agcggcggag agtctcgctg tcgacccggc catcgccgtg ggaaatgatc tccagggcga
    21181 tgtcgtagat ccgcggcagg ccggccgacg ggccattcgc caggcgcggc aattcgcggc
    21241 tgtagccctt gggcagatgc ttctgcgcga tgcggatctg ttcctcaatc aggtagaagt
    21301 tgtcgagcaa ccattccgct gcgggcatga cccgtcgagt ggacagggtc gcggtagtca
    21361 gcgtttcgca gctgcgcacc agcagggctt cgttgtcgcc cagccgcgcc agcagcgagt
    21421 gcgacgccga gcgcgagctc aagcggtgtt cgcgcgccag ctgggcgcca tggctggcca
    21481 tctgctcggc gctgaacaac tcggcgcgca aggcgggctc gtactcgaac gtgcgaggcg
    21541 agccgcgcca cagacggaac ccggtttcca gtcggcgcaa ctgaagcaag gtgagaattc
    21601 gctgaatgtt catcgcttcc cggccggcag gctgaaccgt gaaagagatt gtttagagta
    21661 gcgaagtatt tccagcctgc tggcgaggat ggataggcgg gagattgcag ccctgccgga
    21721 gtggcgaaaa tagccggacc aaacggggag aaggcgcgga aaagtgcgcg ccacagcgca
    21781 gcctggcgcc agccgccgcc tcagcgcagg cacttgcaaa gggccggctt caggctcgct
    21841 gcgtcagtgg ttgaccgtgc aggccagcat catcgacagc tggcacagcg gccgtccgct
    21901 ctgctcctgc cactggttga aggccgcctg caccgccgcc aggtcgcgct ggctggtcgg
    21961 cgccttgtcg acgatgccct gcgccttgag cgcagcgacc acatcgtcgg tgggaatgaa
    22021 ggtgtccttg ccggccatgc gcaggaagcg cggcgcggac aggccgccga gctggcagcc
    22081 gtgcttgctc aggtagcgcc acagaccggt gatgtcgctc gccggccagt cggccagcag
    22141 ggcgccgaag ctgccatgct cgcgcgccac gtcgaggacg aactgcgcgt tgcgcggcac
    22201 gctcttcagc ttggcgaggt ggcggatcag gcgcgcgtcc tgcatcagcc gctcgagatg
    22261 ctcggcgccc atcagcacca ccttgtgcgg atcgaatccg aagaacacct cctcgaaggc
    22321 cggccacttg ccgtccacca cgctgtgctt gagcccggcg cggaagatgc gcaggctgag
    22381 cagcgacagg tagcggtcgt cgctgaccgc ccgcagctcg gcggccgatt tcggctgcgg
    22441 caggtgcgcc tccagcgcgg cgagcgaacc gaagcggttc aggcagtatt cgtgcagcca
    22501 agggtaatcg cgcattgccg ctccgctcgc gtagcaaaag aagcggcaag gttgcccgca
    22561 agcggcgccg ccctgcaagc ccggcacggc tcgcgaggct tcctcctcag ccgcgcagcc
    22621 gctcggcggc gcgcaacagg ctctgctcgg tggccgccca gcccaggcag ccgtcggtga
    22681 tcgacacccc gtagcgcagc gcgccggaca gcggctggca gccgtcgaac agatggcttt
    22741 ccagcatcac cccccgcagg ctggcatcgc cggccaggcg ctggtcgagc acatcctcca
    22801 gcacctgcgg ctggcgcagc ggatccttgc cgctgttggc gtgactgcaa tcgaccacga
    22861 tccgcgccgg gatgccctgg cgctcgagcg cgacgcgggc ggcggcgacg ctgccggcgt
    22921 catgattcgg tccgccgtgg ccgccgcgca gcaccagatg ggtatccggg ttgccgcggg
    22981 tttccagcag cgccgggtgg ccgagggcgt cgatgccgaa gtggcgatgc gcatgcgccg
    23041 ccgagcgcat ggcgtcgcag gcgatgccca ggctgccgtc ggtgccgttc ttgaagccca
    23101 ccggcaggtc caggccgctg accatctcgc ggtggatctg cgactcgctg gtgcgcgcgc
    23161 cgatcgccgc ccagccgagc agatcctcga agtagccggc cgccagcggc tgcagcagtt
    23221 cgctggccag cggcaggccg agctcgagga tgtccagcat caaccggcgc gacagcgcca
    23281 gccccgctgc catgtcgccg ctgccgtcca gatgcgggtc gtagaccagt cccttccagc
    23341 cgatcgtggt acgcggcttc tcgacgtagg cacgcatcac cagcagcagc cggtcgtcga
    23401 cctgaggcgc cagcgcggcc aggcgtgcag cgtagtcgag ggccgactcg gcatcgtgca
    23461 gcgagcaggg cccgagcacc accagcaggc gcgggtcctg gccgtcgagc acggcgcgga
    23521 tgg
//
LOCUS       DATCUN010000190        11925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_120783, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000190 DATCUN010000000
VERSION     DATCUN010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120783"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VIO83_07595"
     CDS             <1..220
                     /locus_tag="VIO83_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidoreductase"
                     /protein_id="HEY8331174.1"
                     /translation="DNIYYSQGCSGHGVTYTHLAGKVLAEALRGQAERFDAFAGLRHY
                     PFPGGRLFRVPFTALGAWYYQLRDRLGA"
     gene            complement(281..1243)
                     /locus_tag="VIO83_07600"
     CDS             complement(281..1243)
                     /locus_tag="VIO83_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HEY8331175.1"
                     /translation="MHQSLRLKALGLLLAGLLLGGCAGSPQLPAEVRLLPERVELSDV
                     PFFPQAEYQCGPAALATMLNQRGVITTPGLLKDRVFLPGREGSLQVELVAAARDNDLL
                     VYPLAPRLDALLAEVAAGNPVLVLQNLAFDWYPRWHFAVLVGYDRHKRELILRSGTTR
                     RWVTDFASFDATWARGGRWAVLTLPPERLPAQAELRPWLQAAADLEQTGHARAAGRAY
                     QAAVQQWPSEGVGWFALGNARYATKDSSGAQVALRQAVAVEPSLAPGWGNLAHVLAEQ
                     GCAGQAEQAQQCARRLAPQDARLREALPATGGGRNCPNLPACPQ"
     gene            complement(1228..1626)
                     /locus_tag="VIO83_07605"
     CDS             complement(1228..1626)
                     /locus_tag="VIO83_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HEY8331176.1"
                     /translation="MFKRAVSRRLAAVLAASQLLLVVQVPVAQASMIGTTEVLQAQQQ
                     QVDRQQLLGMLEDRGVQDKLASLGVERDQVEQRIQSLTNAELAQFNQQLSEAPAGGII
                     GIIVLFLVIFIITDLLCATDIFSFVKCINR"
     gene            1950..3782
                     /locus_tag="VIO83_07610"
     CDS             1950..3782
                     /locus_tag="VIO83_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY8331177.1"
                     /translation="MTSSNTPEQADTPPVKRAEQARGDFAVVRDLLPYLRPYLGRILL
                     ALGLVVAAKLLNLLVPIALKQIVDGLNVEPSLLLLPVGLLLAYGTSRIGVTLFTELRQ
                     VVFARVMARTSRQVTLQVFRHLHGLSLKFHLGRRTGGVARDVERGGSAIADLLDWTLY
                     SIIPILLEVILVTAVLVWAYDWRFAAITLATLLVYGLWTFAVTEWRTRYYRASVEADT
                     RANERAVDSLLNYETVKYFNNEEHEAARYDENLRHLENAKVKATTSLAALNLGQAAVV
                     AGGVTAMMWLAAAGVVDGSMTVGDLVLVNAYLLQLSAPLFLLGMMYREVKQALTNMER
                     LFGLLDERQDVQDAPDATPLATTQPRVRFEDVRFGYDPRRQILHDIDFEIPAGGTVAV
                     VGHSGCGKSTLARLLYRFYDVDGGRITIDGHDLRQLTQASLRGAIAIVPQDTVLFNDS
                     IYYNILYGRPDASREEVEAAARAAHIHDFVQGLPDGYDTPVGERGLKLSGGEKQRVAI
                     ARAILKNPSILIFDEATSALDSQSERAIQAELERIQVGRTTLVIAHRLSTVMNADQIL
                     VMDGGRIAERGSHPQLLALNGQYAQMWRLQQQRAEQSAAEQQHL"
     gene            3852..4367
                     /locus_tag="VIO83_07615"
     CDS             3852..4367
                     /locus_tag="VIO83_07615"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEY8331178.1"
                     /translation="MPGQMHAIQADLTTLAVNAIVNAANPSLLGGGGVSGAILRAGGP
                     QLVEECRRLGGCATGDAKLTRGYRLPAKFVVHTVGPVWQGGNRHEADLLASCYRRALA
                     LAAEAGARTLAFPNISTGIYGYPFEEAAAIAVATVRSALASHPSIESVTFCCFSAADL
                     AVYTRILEASA"
     gene            4453..4974
                     /gene="phaR"
                     /locus_tag="VIO83_07620"
     CDS             4453..4974
                     /gene="phaR"
                     /locus_tag="VIO83_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEY8331179.1"
                     /translation="MPDRNHVRLIKKYANRRLYDTQTSTHITLSDIRKLVIQEESFQV
                     VDAKTGEDLTRSILLQIIQEAESDGEPIFSSEMLKGIIRFYGPFQGMLGSYLDKSIQA
                     VVDIQAQTGQQSSQAWNDFMHSQIPTMQKLMGQYVEQSKQLYLNTQNLFGMFPGFPGT
                     AGRGDKDKDDDKS"
     gene            complement(5058..5627)
                     /locus_tag="VIO83_07625"
     CDS             complement(5058..5627)
                     /locus_tag="VIO83_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY8331180.1"
                     /translation="MSFFDSEKLQAAQKTNLEVLEQVNGKVLKGLEQLSRLQIAALRS
                     ASEEHFENARKLLTVRDPQSFTSVFSAFANPAHQAERLMEFNRQVYDLFSSTQADIAK
                     LGERQVAQNAQQVQELVEEIAKNAPAGSESAVAVLKTAVETASNVYESAQKTAKQAAE
                     IAENGINAAASAAGQATRDATKAAGAARK"
     gene            6108..6851
                     /locus_tag="VIO83_07630"
     CDS             6108..6851
                     /locus_tag="VIO83_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HEY8331181.1"
                     /translation="MTNKRIALVTGGMGGIGTAISRRLAREGFVVVVGCSPQSTRKDA
                     WLKQQQQDGFEFHCMTSDITDWDDTVRAFAKVREEVGQIDVLVNNAGITRDTTFKKMT
                     PQDWSAVISTNLNGLFNTTKQVVDSMVERGWGRIINISSVNGQRGQFGQTNYSAAKAG
                     IHGFSMALAREVASKGVTVNTISPGYIRTEMTAVIREDVMEKIVSGIPVGRLGQPEEI
                     ASMIAWLASDEGAYATGADFSVNGGLNMA"
     gene            6940..8118
                     /locus_tag="VIO83_07635"
     CDS             6940..8118
                     /locus_tag="VIO83_07635"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017902125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY8331182.1"
                     /translation="MNDVVIVAATRTAIGSFQGGLSSISAPDLGAAVIRQVLEQTGVQ
                     PSAIDEVILGQVLAAGSGQNPARQTAIKAGLPHEVPALTLNKVCGSGLKAVQLAVQAI
                     RCGDAELVIAGGQENMSLSPYVLPKARTGLRMGHAELVDSMIKDGLWDAFNDYHMGVT
                     AENLAQRFDISRAQQDAFAAASQQKAAAAIEAGRFKDEITPILIPQRKGDPLVIATDE
                     QPRGDTTLDSLSKLKPAFSKDGSVTAGNASSLNDGAAVVLLASAKKAAELGLPVLAKI
                     TAYASAGVDPAIMGIGPVSATRRCLEKAGWSIDDLDLVEANEAFAVQALSVNKELGWD
                     TDKVNVNGGAIALGHPIGASGCRILVTLVHELQRRNLSKGLATLCIGGGQGVALAIER
                     "
     gene            8208..9914
                     /gene="phaC"
                     /locus_tag="VIO83_07640"
     CDS             8208..9914
                     /gene="phaC"
                     /locus_tag="VIO83_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEY8331183.1"
                     /translation="MDNAPPFTAFFSANMPFVARAALEQLRTWVRHNPWFQGNNTDAW
                     FEVDSAILDRLQAEYSGEWAQLGMQVLTLQPFTFSDSRFTGSAWSQPLFGSLAALYLL
                     NSRFLMRLLDELPIESGKMRQRLTFLVEQLIAAGAPSNYLNSNPEALERAVATQGASL
                     FSGLLHLASDMHEGKLRQCDSGAFEVGVNLATTPGQVVFQNPLLQLIQYAPQGETQFK
                     RPLLIVPPAINKFYILDLQPESSLVRYALEQGHPVFLVSWRNADASIANATWDDYVEK
                     GIIEAIKVTQSISGQEQLNLLGYCVGGTLLSCGLGVLAARGEKPAASLTLLVTLLDFE
                     DAGVLNVFVDEEIVSYRERTIGGGGGNYGLFRGEDMGNTFSLLRPNELWWNYGVEKYL
                     KGQKPRPLDLLYWNNDSTHLPGPMYCWYMRHTYLQNDLKSGKQQVCGETLDLGKLDMP
                     TYLVGTREDHIVPWRGAFASSRLLKGPTRFVLGASGHIAGVINPPAKKKRSYWTNTEL
                     PGAPDDWFAGAEEHAGSWWGDWAEWLAKTSGERGVPVTNLGSADYPPLEAAPGSYVKQ
                     RD"
     gene            complement(9963..10634)
                     /locus_tag="VIO83_07645"
     CDS             complement(9963..10634)
                     /locus_tag="VIO83_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8331184.1"
                     /translation="MSGIQKSAQRSLSLEVGDVLLQGDLTLPDAAEGLVLFAHGSGSS
                     RFSPRNQQVARYFNDLGLATLLLDLLTEDEQDVDEVTRQFRFDIALLARRLTGVLDWL
                     QQEERLQELVIGLFGASTGAAAALICAAERPHSVAAVVSRGGRTDLAGAALERVRAPT
                     LLIVGGEDDEVLALNRESLLRLGGIQHLGVVAGATHLFEEPGKLLVVAELAGEWFLRY
                     LRQVP"
     gene            10870..11358
                     /locus_tag="VIO83_07650"
     CDS             10870..11358
                     /locus_tag="VIO83_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8331185.1"
                     /translation="MNVCEVMHKPAQCCHSSDSLELAAQLMAQNDCGAIAVVDQANHA
                     IGIVTDRDIALLAAERHQPLWELQAGELTGKHNLYTCHDDTDVHEALHMMADRKVRRL
                     PVVGADGELHGMVAMSDILHGLRMSGASVQAAPEDWREALETFNHITTPSLPINMPDK
                     DM"
     gene            complement(11407..>11925)
                     /locus_tag="VIO83_07655"
     CDS             complement(11407..>11925)
                     /locus_tag="VIO83_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY8331186.1"
                     /translation="VLVVAGGCGLAPLAGWIEEALRHPAPPQLRVLYGARHAEAQVLG
                     RERARWRAVLPLIETLDQGGAEALRGSPLAQLDRLFAEEVPQVVLCCGPEAFMLAIAK
                     ACVRRGVAADSIWLSVERRMHCAVGLCGHCYIADSYACVDGPTYRYDQYLTLLARGNG
                     RGALGELGVGPC"
BASE COUNT         2241 a         4165 c         3589 g         1930 t
ORIGIN      
        1 cgacaacatc tactactcgc agggctgcag tggccatggg gtgacctaca cgcacctggc
       61 cggcaaagtg ctggccgagg ccctgcgcgg tcaggccgaa cgtttcgacg cctttgccgg
      121 cctgcgccac taccccttcc ctggcggccg gctgttccga gtgccgttca ccgcactggg
      181 tgcctggtac tatcagctgc gcgaccggct gggcgcctga ataaagcacc caaagaccaa
      241 cggcacccga aaggtgccgt tggtctttgg gtgctggccg ctactgcggg caggcaggca
      301 agttcgggca gttcctgccc ccccctgtag ccggcagcgc ctcgcgcaag cgtgcatcct
      361 gtggcgccag ccggcgcgcg cattgctgcg cctgctccgc ctgcccggca cacccctgct
      421 ccgccagcac atgagccagg ttgccccaac ccggcgccag cgacggttcg accgcgacgg
      481 cctggcgcag cgcaacctgt gccccggagg aatccttcgt cgcgtagcgg gcattgccca
      541 gcgcaaacca gcccactccc tcactcggcc attgctgcac ggcggcctga taggcacgac
      601 cggctgcgcg cgcatgcccg gtctgctcca gatcggcggc ggcctgcaac caggggcgca
      661 gctcggcctg cgccggcaac cgctccggag gcagggtcag caccgcccag cgtccgccac
      721 gcgcccaggt cgcgtcgaag ctggcgaagt cggtcaccca gcgccgggtg gtgccggagc
      781 gcaggatcag ctcgcgcttg tgccggtcgt agcccaccag caccgcgaag tgccagcgcg
      841 gataccagtc gaacgccagg ttctgcagca ccaggaccgg attccccgcc gccacttcgg
      901 ccagcagggc atccagccgc ggcgccagcg ggtagaccag cagatcgttg tcgcgcgccg
      961 ccgccaccag ctcgacctgc aggctgccct cgcgtccggg caggaacacc cgatccttga
     1021 gcaagcccgg cgtggtgatc acgccgcgct gattgagcat ggtcgccagc gcggcggggc
     1081 cgcactggta ctcggcctgc gggaagaacg gcacgtcact cagctcgacc cgctccggca
     1141 atagacgaac ctctgccggc agctgcggcg aacctgcgca gccaccgagc agcaggccgg
     1201 ccagcagcaa gccgagcgcc ttcagcctca acgattgatg catttgacga aggagaagat
     1261 gtcagtcgcg caaagcaggt cggtaatgat gaagatcacc aggaacaaca cgatgatacc
     1321 gatgatgccg cccgccggtg cctcgctgag ctgttgattg aactgcgcca gctcggcgtt
     1381 ggtcaggctc tggatgcgtt gctcgacctg gtcccgctcg acccccaggg acgccagctt
     1441 gtcctgcacc ccgcggtcct ccagcatgcc cagcagctgc tggcggtcga cctgctgttg
     1501 ctgcgcctgc aggacttccg tggtgccaat catcgaggct tgcgctaccg gcacctgaac
     1561 caccagcaac agctgggaag ccgccagcac ggcagccaat cgacgagaaa ctgctcgctt
     1621 gaacatggga cgctcctttt attgtttcct gtggatccgc tctgacacgt gcaggacagt
     1681 agggttcctc tgcagaatag gttatgaggc tgcaacgtgt cgcccttcac gccccgccat
     1741 ccctgtcaat gtgcacctgt tcaccgcagg catggccagc cgatcgcagc gcattcctgt
     1801 cgatgagcag agccttaccc tgggcacctg gcggaccagc gcctttttca cgttgcacga
     1861 cagccgcgag ccgcccagca tagcccaccc cgatgaatcg atttgccgag ccaacccacc
     1921 tcggcaagcg ccacaggaac ccggtgacga tgacgtccag caatacccca gaacaagccg
     1981 acaccccacc cgtcaaacgt gccgagcagg cacgcggcga ctttgccgtg gtccgcgacc
     2041 tgctgcccta cctacgcccc tatctgggac gcatcctgct ggccctgggc ctggtggttg
     2101 ccgccaaact gctcaacctg ctggtgccga tcgcgctcaa gcagatcgtc gacggcctca
     2161 acgtcgagcc cagtctgttg ctattgccgg tcggcctgct gctggcttac ggcacctcgc
     2221 gcatcggcgt caccctgttc accgagctgc gccaagtggt gttcgcccgg gtcatggcac
     2281 gcacctcgcg gcaggtcacc ctgcaggtat tccgtcactt gcacggcctc agcctgaagt
     2341 tccacctcgg ccggcgtacc ggcggggtgg cgcgggatgt cgagcgcggt ggcagcgcca
     2401 tcgccgacct gctcgactgg accctgtact cgatcatccc gatcctgctg gaggtgatcc
     2461 tggtcaccgc cgtgctggtg tgggcctacg actggcgctt cgccgccatc accctcgcca
     2521 ccctgctggt ctacggcctg tggaccttcg ccgtcaccga atggcgcacc cgctactacc
     2581 gcgcctcggt ggaggccgac acccgcgcca acgagcgcgc cgtggactcg ctgctcaact
     2641 acgagacggt caagtacttc aacaacgagg aacacgaggc ggcgcgctac gacgagaatc
     2701 tgcgccacct ggagaatgcc aaggtcaagg ccaccaccag cctggctgcg ctcaacctcg
     2761 gccaagctgc cgtggtcgcg ggcggcgtca ccgcgatgat gtggctggcc gctgccggcg
     2821 tcgtggacgg cagcatgacg gttggcgatc tggtgctggt caacgcctac ctgctgcagc
     2881 tttccgcccc gctgttcctc ctcggcatga tgtaccgcga ggtcaagcag gccctgacca
     2941 acatggagcg cctgttcggc ctgctcgatg agcgccagga tgtgcaggac gcgccggacg
     3001 ccacgccgct ggccaccacc cagccacggg tgcgcttcga ggacgtgcgc ttcggctacg
     3061 atccacgccg gcagatcctc cacgacatcg acttcgagat ccccgcaggc ggtacggtgg
     3121 cggtggtcgg ccactccggc tgcggcaagt cgaccctggc gcgcctgctg taccgcttct
     3181 acgacgtgga cggcggacgc atcaccatcg acggtcacga cctgcgccag ctgacccagg
     3241 cctccctgcg tggggccatc gccatcgttc cgcaggacac cgtgctgttc aacgacagca
     3301 tctactacaa catcctctac ggccgccccg acgccagtcg cgaggaagtg gaagccgctg
     3361 cccgtgccgc gcacatccac gacttcgtgc agggcctgcc ggacggctac gataccccgg
     3421 tcggcgagcg cggcctcaag ctctccggcg gcgagaagca gcgcgtggcg atcgcccgcg
     3481 cgatcctcaa gaaccccagc atcctgatct tcgacgaagc cacctcggcg ctggactcgc
     3541 agtccgagcg cgccatccag gccgaactgg agcgcatcca ggtcggccgc accaccctgg
     3601 tcatcgccca ccgcctgtcc acggtgatga atgccgacca gatcctggtg atggacggtg
     3661 gacgcatcgc cgagcgcggc agccacccgc aactcctggc cctgaacggc cagtacgccc
     3721 agatgtggcg cttgcagcag cagcgcgcgg agcagtcggc agcggagcaa cagcacttgt
     3781 gacgcgctac gcacggccgg cgccgatggc ctaatgtcag acaggacccg acaccgccgc
     3841 gagcacccgc catgcccgga caaatgcacg ccatccaggc cgacctcacc accctggcgg
     3901 tcaacgccat cgtcaacgcc gccaaccctt cgctactcgg cggcggtggg gtctccggcg
     3961 ccatcctgcg cgcgggcggg ccgcaactcg tggaggaatg ccgccgcctc ggtggctgcg
     4021 ccaccggcga cgccaaactg acccgcggct atcgtctgcc ggccaagttc gtcgtgcaca
     4081 cggtcggccc cgtctggcag ggcggcaacc gccacgaggc ggacctgctc gcctcctgct
     4141 accgacgcgc tctcgcgctc gccgccgagg ccggtgcgcg caccctggcc tttccgaaca
     4201 tcagcaccgg catctacggc taccccttcg aggaggcagc agccattgcg gtggccacgg
     4261 tccgctcggc attagcgagc caccccagca tcgagtcggt aacgttctgc tgtttctccg
     4321 ccgccgatct tgcggtgtat acccgcatcc tcgaggcaag tgcctgagca cgagcccagc
     4381 aagcagcgac tcggggcgtg cgcttttggg ttaacatgag ttgaaacctg cccttgcccg
     4441 agagtccgca gcatgcctga tcggaaccat gtccgcctga tcaagaaata cgccaaccgt
     4501 cgtctctacg acacgcagac cagcacccac atcacgctga gcgatatccg caagctggtc
     4561 atccaggaag aatcattcca ggtggtggat gccaagaccg gcgaagacct gacgcgcagc
     4621 atcctgctgc agatcattca ggaagccgaa agcgacggcg agcccatctt ctccagcgaa
     4681 atgctcaagg ggatcatccg cttctacggc cccttccagg gcatgctcgg cagttacctg
     4741 gacaagagca tccaggcggt ggtcgacatt caggcccaga ccggccagca atcgtcgcag
     4801 gcgtggaacg acttcatgca cagccagata ccgacgatgc agaagctgat ggggcagtat
     4861 gtggagcagt cgaaacagct gtacctgaac acgcagaatc tgttcggcat gttcccgggc
     4921 ttccccggca cggctggtcg cggcgacaag gacaaggacg acgacaagtc ctgattcgca
     4981 cagttagata acactccgca ccaaatatgc acaaagcccc ggcatgaccg gggctttgtg
     5041 cattcatcag gcttggctta cttgcgagcc gcaccagctg ccttggtggc atcacgggtc
     5101 gcctgaccag cggccgaagc agcggcattg atgccatttt cggcgatttc ggctgcctgc
     5161 ttggcggttt tctgcgcgct ttcatagacg ttgctggcag tctcgacagc agtcttcagc
     5221 acagccacag ccgactcgga gccagctggc gcattctttg caatttcctc taccagttcc
     5281 tgcacctgct gagcgttctg ggcaacctga cgctcaccga gcttggcgat gtcggcctgg
     5341 gtgctggaga acaggtcgta cacctggcgg ttgaactcca tcagacgctc ggcttgatgg
     5401 gccggattgg cgaaggcact gaacacggag gtgaagcttt gcggatcacg aacggtgagc
     5461 agcttgcggg cgttttcgaa gtgctcctcg ctggcggagc gaagcgcggc gatctgcagg
     5521 cggctgagct gctcgagacc cttgagcacc ttgccattca cctgctcgag cacttcgagg
     5581 ttggttttct gagcggcttg cagtttttcc gaatcgaaaa aggacatgaa tgaatctcca
     5641 gaggatgaaa ttaaggtcgg gaaagccgtg ctgttgtgaa ttcattcacc cgacacgcaa
     5701 atctattctt ggcgattcct gagcgacgcg caactgtttt tttgcactgc acaaaaaaat
     5761 tttgcccacc tcagcaaccg acaacccggc gcaagatctc cgcaaacggt gatcaagccc
     5821 cctgaaatag caagaccaag tgacgatcct gtgaatgcga ggtaatcaat agacccagag
     5881 agaccagccg attttgcaca gcaacagccg cgagaccgcg gaacaacggc actctgaggc
     5941 tcatccgccc cagaaacgtt caaaaaaacg taacaaccca caagcaatgt ttatccatcg
     6001 ggacccaaac agaattagcc cgaagctaga ccacagatcg gcgcagcatg cgccgctcga
     6061 tccggcaagg atgtcccggt tttcaactca ccactggagt aaagacaatg actaacaagc
     6121 gtattgcact ggtgactggc ggtatgggtg gcatcggcac ggcgatcagc cgtcgcctgg
     6181 cccgtgaagg ttttgtagtg gtggttggct gcagcccgca gtcgacccgc aaggatgcct
     6241 ggcttaagca gcagcagcag gacggtttcg aatttcattg catgaccagc gacattaccg
     6301 actgggacga caccgtgcgc gccttcgcca aggtgcgcga ggaggtcggc cagatcgacg
     6361 tactggtcaa caacgcgggc atcactcgcg ataccacgtt caagaagatg accccgcagg
     6421 actggagcgc ggtgatttcc accaacctca acggcctgtt caacaccacc aagcaggtag
     6481 tcgactcgat ggtcgagcgc ggctggggcc gcatcatcaa catctcttcg gtcaacggcc
     6541 agcgcggcca gttcggccag accaactact cggcagccaa ggccggcatc catggtttca
     6601 gcatggcgct ggcccgtgaa gtggccagca agggcgtcac cgtcaacacc atctccccgg
     6661 gctacatccg caccgagatg accgcggtga tccgtgagga cgtgatggag aagatcgttt
     6721 ccggcatccc ggtcggccgc ctcggccaac cggaggagat cgcctccatg atcgcctggc
     6781 tggcctccga cgagggtgcc tatgccaccg gcgcggactt ctcggtcaac ggcggcctga
     6841 acatggccta agtcggcccc ggcatgctct gcaaccgccc ggcatcctct ccggcaccgg
     6901 gcggtcgtct cccttcttgc ttgtagtcga ggtctttata tgaacgatgt tgtcattgtc
     6961 gccgctaccc gtaccgccat cggcagcttt cagggtggcc tgagctccat ttccgccccc
     7021 gacctgggcg ccgcggtgat ccgccaggtg ctggaacaaa ccggcgtgca gccgagcgcc
     7081 atcgacgaag tgatcctcgg ccaggtcctc gccgccggct ccggacagaa cccggcccgc
     7141 cagaccgcga tcaaggccgg cctgccccat gaggtgccgg cactgaccct gaacaaggtc
     7201 tgcggctccg gtctcaaggc cgtacagctg gctgtccagg ccatccgctg tggcgatgcc
     7261 gagctggtga tcgctggcgg ccaggaaaac atgagcctgt cgccttacgt cctgcccaag
     7321 gcccgcaccg gcctgcgcat gggccatgcc gagctggtcg acagcatgat caaggacggc
     7381 ctgtgggatg ccttcaacga ctaccacatg ggtgtcaccg ccgagaacct ggcccagcgt
     7441 ttcgatatca gccgtgcgca gcaggacgcc ttcgccgccg cgtcccaaca gaaggccgcc
     7501 gccgctatcg aggccggtcg cttcaaggac gagatcaccc caatcctgat cccccagcgc
     7561 aagggtgatc cgctggtaat cgccaccgac gaacagccgc gcggcgacac caccctggac
     7621 agcctgagca agctcaagcc ggccttcagc aaggatggca gcgtcaccgc cggcaacgcc
     7681 tcgtccctca acgacggcgc cgcggtggtg ctgctcgcta gcgcgaagaa ggccgccgaa
     7741 ctgggtctgc cggtgctggc gaagatcacc gcgtatgcca gcgctggcgt cgacccggcg
     7801 atcatgggca tcggtccggt ctcggccact cgccgctgcc tggaaaaggc cggctggagc
     7861 atcgacgacc tcgacctggt cgaagccaac gaagcctttg ccgtgcaggc gctgagcgtc
     7921 aacaaggaac tgggctggga taccgacaag gtcaacgtca acggcggcgc aatcgccctt
     7981 ggccacccga tcggtgcttc cggctgccgc atcctggtga cgctggtgca tgagctgcag
     8041 cgccgcaacc tgagcaaggg cctggcaacc ctgtgcatcg gtggcggtca gggtgtcgcg
     8101 ctggcaatcg aacgctgata tggtctgaac gccctcgccc cggccatgtc cggggcgaga
     8161 gcgttgccaa tcggacacca gcaaccccca gatagcggac acaagaaatg gataacgccc
     8221 cgcccttcac ggcctttttc tcggccaaca tgcccttcgt cgcccgcgca gccctagagc
     8281 aactgcgcac atgggtacgc cacaacccgt ggttccaggg caacaacacc gatgcctggt
     8341 tcgaggtgga cagtgccatc cttgaccgcc tgcaggcgga atacagcggc gaatgggcgc
     8401 aactcggcat gcaggtactg accctgcagc ccttcacttt cagcgattcg cggtttaccg
     8461 gcagcgcctg gagccaaccg ttgttcggct cgctggcggc cctttacctg ctcaactcgc
     8521 gctttctcat gcgcctgctc gacgagctgc cgatcgaatc cggcaagatg cgccagcgcc
     8581 tcaccttcct ggtggaacaa ctgatcgctg ccggtgcgcc gagcaactac ctgaacagca
     8641 acccggaagc cctcgagcgc gcggtggcaa cccagggcgc cagcctgttc agcggcctgc
     8701 tgcacctggc cagtgacatg cacgaaggca agctgcgcca gtgcgacagc ggcgccttcg
     8761 aggtgggtgt caatctggcc accacccccg gccaggtggt cttccagaat ccgctgctcc
     8821 agctgatcca gtacgcaccg cagggcgaga cccagttcaa gcgtccgctg ctgatcgtgc
     8881 cgccggcgat caacaagttc tacatcctcg acctgcagcc ggaaagctcg ctggtgcgct
     8941 atgccttgga gcagggccac ccggtcttcc tggtttcctg gcgcaacgcc gacgcgagca
     9001 tcgccaatgc cacctgggac gactacgtcg agaagggcat catcgaggcg atcaaggtca
     9061 cccaatccat cagcgggcaa gaacagctca acctgctcgg ctactgcgtc ggcggcaccc
     9121 tgctctcctg cggtctgggc gttctcgccg cccgtggcga gaagccggca gcgagcctga
     9181 ccctgctggt caccctactc gacttcgagg atgccggcgt actcaacgtg ttcgtcgacg
     9241 aggagatcgt cagttaccgc gagcgcacca ttggcggtgg cggcggcaac tacggcctgt
     9301 tccgcggcga ggacatgggc aacaccttct ccctgctgcg ccccaacgag ctgtggtgga
     9361 actacggcgt cgagaagtac ctcaagggcc agaagccgcg tccgctggac ctgctgtact
     9421 ggaacaacga cagcacccac ctgcccggcc cgatgtactg ctggtacatg cgccacacct
     9481 acctgcagaa cgacctgaag agcggcaagc aacaggtctg cggcgaaacc ctcgacctgg
     9541 gcaagctcga catgccgacc tacctggtcg gcacccgcga ggaccacatc gtgccctggc
     9601 gtggcgcctt tgccagcagc cgtctgctca agggcccaac ccgtttcgtc cttggtgcct
     9661 ccgggcacat cgccggggtg atcaacccgc cggccaagaa gaagcgcagc tactggacca
     9721 acaccgaact gccgggtgct ccggacgact ggtttgccgg tgccgaagag catgccggca
     9781 gctggtgggg tgactgggcc gagtggctgg ccaagacctc gggcgaacgc ggcgtgcccg
     9841 tcaccaacct gggctccgcc gactacccgc cgctggaagc ggcgcccggc agctatgtga
     9901 aacagcgcga ctgacccgct gcagcgtccc cggccagccg gccggggacg ctcctcttcg
     9961 cctcagggca cctggcgcag atagcgcagg aaccactccc ccgccaactc ggccaccacc
    10021 agcagcttgc ccggttcctc gaacagatgc gtggcgcctg ccaccacccc cagatgctgg
    10081 ataccgccca agcgcagcaa gctctcgcga ttgagcgcca gcacctcgtc atcctcacca
    10141 cccacgatca gcagcgtcgg cgcacggacc cgctccagag ccgcaccagc cagatcggtg
    10201 cgcccgccac gggacaccac cgcagccaca ctgtgaggac gctccgccgc acagatcagc
    10261 gccgccgccg caccggtact ggcaccgaac agcccgatga ccagctcctg caagcgctcc
    10321 tcctgctgca gccagtcgag cacgcccgtc aggcgtcgcg ccagcagcgc aatgtcgaaa
    10381 cggaactgac gggtcacctc gtcgacatcc tgctcgtcct cggtgagcag atcgagcagc
    10441 agggtggcca gccccaggtc gttgaagtag cgcgccacct gctggttgcg cggactgaag
    10501 cggctgctgc cgctgccatg ggcgaacagc accagacctt cggccgcgtc cggtagcgtc
    10561 agatcgccct gcagtagcac atctccgact tcgaggctca ggctacgctg cgctgacttt
    10621 tggattccgc tcatgactcc accccattga tcgagttgcc ggccgtggcc catcgaaact
    10681 ctgcatgaag tctagatgtt tggtcgcacc gccagcggac aacaggcccg cacttgatca
    10741 atatcaaggc agccgtgccg cagtagcgcc caatgaatga ttctgttccc gtcaggagta
    10801 cacgggttca ttcgcccggt caccgccggc aagggctgcc cacgatgacc cctatgaagg
    10861 agcgggatca tgaacgtctg cgaggtaatg cataagccgg cccagtgctg tcatagcagc
    10921 gacagcctgg agctggccgc gcaactgatg gcgcagaacg attgcggcgc catcgccgtg
    10981 gtcgaccagg ccaatcatgc catcgggatc gtcaccgacc gcgatatcgc cctgctggcg
    11041 gccgaacggc accagccgct gtgggaactg caagccggcg agctgaccgg caagcacaac
    11101 ctgtacacct gccacgacga caccgacgtg cacgaggcgc tgcacatgat ggcggaccgg
    11161 aaagtacgcc gcctgccggt ggtcggcgcc gatggcgagc tgcatggcat ggtcgccatg
    11221 tccgacatcc tccatggcct ccgcatgtcc ggtgccagtg tgcaggcagc gcccgaggac
    11281 tggcgggaag cgctggagac cttcaaccac atcaccacgc cgagcctgcc gatcaacatg
    11341 cccgacaagg acatgtagct tggctcggca taaccggccc ggattcgccg cgaggcgatc
    11401 cgggcttcag caaggcccca cccccagctc gccgagcgcg ccgcggccat tgccccgcgc
    11461 gagtaacgtc aggtattggt cgtagcgata ggtcggccca tcgacacagg catagctgtc
    11521 ggcgatgtaa cagtgcccgc agagtcccac ggcgcagtgc atgcgccgct ccaccgacag
    11581 ccagatgctg tccgccgcca ccccgcgccg cacacacgcc ttggcgatgg ccagcatgaa
    11641 cgcctccggg ccacagcaca gcaccacctg cggcacttcc tcggcgaaca ggcgatccag
    11701 ctgggctagc ggcgagccgc gcagcgcctc ggccccaccc tggtcgaggg tttcgatcag
    11761 cggcagcacc gcgcgccagc gcgcccgttc acgtccgagc acctgagcct cggcgtggcg
    11821 cgcaccgtag agcacgcgca gctgcggtgg cgcgggatgg cgcagggctt cctcgatcca
    11881 gccagccagc ggcgccagcc cgcagccacc ggcaaccacc agcac
//
LOCUS       DATCUN010000191         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_158491, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000191 DATCUN010000000
VERSION     DATCUN010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158491"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VIO83_07660"
     CDS             complement(<1..312)
                     /locus_tag="VIO83_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEY8331187.1"
                     /translation="MSQPLRIVFAGTPEFAARHLQALLDAGLTPLAVYTQPDRPAGRG
                     QKLMASPVKQLAVAHGIPVLQPATLRDPAAQAELAALAPDLLVVVAYGLILPQVVLDI
                     PH"
     gene            complement(362..871)
                     /gene="def"
                     /locus_tag="VIO83_07665"
     CDS             complement(362..871)
                     /gene="def"
                     /locus_tag="VIO83_07665"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY8331188.1"
                     /translation="MAILNILEFPDPRLRTLAKPVIEVDDALRQLIDDMFETMYAAPG
                     IGLAATQVNVHKRLVVMDLSEDQSAPRVFINPELETLSEELGPYQEGCLSVPGFYENV
                     DRPLKVRIKALDRDGQPFEEIAENLLAVCIQHECDHLNGKLFVDYLSGLKRDRIKKKL
                     EKQHRQQHS"
     gene            1012..2037
                     /locus_tag="VIO83_07670"
     CDS             1012..2037
                     /locus_tag="VIO83_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY8331189.1"
                     /translation="MRKTLVALLLMAAGSLAQAEVQLKDGHPQNYTVVRGDTLWDISG
                     KFLKQPWKWPEIWQVNPQVQNPNLIYPGDVLSLTYVDGQPRIQLNRGASRGTIKLSPT
                     VRSTPMAEAIPTIPLEKINSFLLSNRILDSEVELSQAPYIVAGNAERVVSGAGDRIYA
                     RGSFADGQPVYGLFRQGKTYTDPQSGEVLGINADYVGSGEVVAEEGDIATLNLSRTNQ
                     EARLGDRLLPTEERAISSTFQPSEPSSEIDGRIIDVPRGVTQIGLFDVVTLDKGARDG
                     LSEGNVLAVYKTGETVRDRITGESVKVPDERSGLLMVFRTYDKLSYGLVLNASRSLAV
                     QDKIRNP"
     gene            2150..>2531
                     /locus_tag="VIO83_07675"
     CDS             2150..>2531
                     /locus_tag="VIO83_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY8331190.1"
                     /translation="MPHPAALSPAELEARLRLHALPEVGPRRFKQLLAAFGSASAALS
                     APASAWQSLGLPAACAVARRSAGVREQVANSLAWLEQPTQHLLCLDDPRYPALLAELD
                     DAPPLLFVAGDPAQLERPQLAMVGS"
BASE COUNT          431 a          855 c          839 g          406 t
ORIGIN      
        1 atgcgggatg tccagcacca cctgcggcag gatcaggccg taggccacca ccaccagcag
       61 gtcgggcgcc agtgccgcca gttcggcctg ggcggccggg tcgcgcaggg tcgccggttg
      121 cagcaccgga atgccgtggg cgacggcgag ctgcttgacc gggctggcca tcagcttctg
      181 gccgcggccg gccgggcggt cgggctgggt gtacaccgcc agcggggtca ggcctgcgtc
      241 gagcagggcc tgcaggtggc gggcggcaaa ttccggggta ccggcgaaga cgatgcgcag
      301 gggctggctc atggggctcg ctcaagaaaa aaggcttgcc gcgggcaagc cttctgatga
      361 atcaggagtg ttgctgacga tgctgttttt ccagcttctt cttgatccgg tcgcgcttga
      421 ggccggacag gtagtcgacg aacagcttgc cgttgaggtg gtcgcactcg tgctggatgc
      481 acacggcgag caggttctcg gcaatttcct cgaagggctg gccgtcgcgg tccagcgcct
      541 tgatgcgcac cttcagcggg cgatcgacgt tctcgtagaa accgggtacc gacaggcagc
      601 cttcctggta ggggccaagc tcctcgctca gggtttccag ctcggggttg atgaacaccc
      661 gcggcgcgct ctggtcctcg gacaggtcca tcaccaccag acgcttgtgc acgttgacct
      721 gggtcgccgc caggccgatg ccgggcgctg cgtacatggt ctcgaacatg tcgtcgatca
      781 gctggcgcag ggcgtcgtcc acctcaatca ccggcttggc gagggtgcgc aggcggggat
      841 cggggaattc gagaatgttc aggatcgcca tatgcgtttg tgatgcactt gtggaataaa
      901 tcgaaaaccg gctgataaga tgcccggcag ctcgaaaaac ggctccaggc cgcgtctttc
      961 gccggccagc gcacgttttg tgtgtcggca catgataaag ggattcacct catgaggaaa
     1021 acattagtcg ccctgctgct gatggcggct ggcagcctgg cccaggccga agtccagctc
     1081 aaggatggcc acccgcagaa ctacaccgtg gtgcgcggcg acaccctgtg ggacatctcc
     1141 ggcaagttcc tgaagcagcc gtggaagtgg ccggaaatct ggcaggtcaa cccgcaggtg
     1201 cagaatccca acctgatcta cccgggcgac gtgctcagcc tgacctatgt cgacggccag
     1261 ccgcgcatcc agctcaaccg cggcgcctcg cgcggcacca tcaagctgtc gcccaccgtg
     1321 cgcagcacgc cgatggccga ggcgatcccg accattccgc tggagaagat caacagcttc
     1381 ctgctcagca accgcatcct cgacagcgag gtcgagttgt cgcaggcgcc gtacatcgtc
     1441 gccggcaacg ccgagcgcgt ggtcagcggc gctggcgacc gcatctatgc ccgcggcagc
     1501 ttcgccgacg gccagccggt ctacggcctg ttccgccagg gcaagaccta caccgacccg
     1561 cagagcggcg aagtgctcgg catcaatgcc gactacgtcg gcagcggcga agtggtggcc
     1621 gaggaaggcg acatcgccac cctcaacctg agccgcacca accaggaagc acgtctgggt
     1681 gaccgcctgc tgcccaccga ggagcgcgcg atcagctcga ccttccagcc cagcgagccg
     1741 agcagcgaga tcgacggccg gatcatcgac gtgccgcgcg gggtaaccca gatcggcctg
     1801 ttcgacgtgg tgaccctaga caagggcgcg cgcgacggcc tcagcgaagg caacgtgctg
     1861 gcggtataca agaccggcga aaccgtgcgc gaccgcatca ccggcgagag cgtcaaggtg
     1921 ccggacgaac ggtccggcct gctgatggtg ttccgcacct acgacaagct cagctacggc
     1981 ctggtgctca acgccagccg ttcgctggcg gtgcaggaca agattcgcaa tccgtaatcc
     2041 cgccctcgtt gcgcgcacga ccgacaacgc ccccgcatgc gggggcgttg tctttttccc
     2101 gccgccatcc taggctcggg cccctgtttc ccgatcaagg atgatcgtca tgccgcaccc
     2161 tgccgccctg tcgccggccg aactggaagc ccgcctgcgc ctgcatgccc tgccggaagt
     2221 cggtccgcgc cgcttcaagc aactgctcgc cgccttcggc tcggccagtg ccgccctgag
     2281 cgcgccggcc tccgcctggc aatcgctcgg cctgccggcg gcctgtgccg tggcgcggcg
     2341 cagcgccggg gtgcgcgaac aggtggcgaa cagcctcgcc tggctggagc agccgacgca
     2401 gcacctgctg tgcctcgacg atccgcgcta cccggccctg ctcgccgagc tggacgacgc
     2461 gccgccgctg ctgttcgtcg ccggcgatcc ggcgcagctg gagcgcccgc agctggccat
     2521 ggtcggcagc c
//
LOCUS       DATCUN010000192         1754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_177663, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000192 DATCUN010000000
VERSION     DATCUN010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177663"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..>1754
                     /locus_tag="VIO83_07680"
     CDS             386..>1754
                     /locus_tag="VIO83_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEY8331191.1"
                     /translation="MLKKLFQSIRSPLRRKRAVRSTPEVLGGSQHSLRREQISRNAVG
                     VVERLQKAGYQAYLVGGCVRDLLLGHQPKDFDVATSATPEQVRAEFRNARVIGRRFKL
                     VHVHFGREIIEVATFRAHHPQGEDDEDSHLASRNESGRILRDNVYGNQEDDAQRRDFT
                     INALYYDPASEHILDYAHGVHDIRNRLVRLIGDPEQRYLEDPVRMLRAVRFAAKLDFD
                     IEKHSAAPIRRLARLLNDIPAARLFDEILKLLLSGQGERTFDLLCEYDLLAPLFPASA
                     AALKADPEYAGRLMRNALANTDERIRQGKPVTPAFLFAALLWPALPARAAALQASGMP
                     ALPALQEAAHEVISEQCRRTAIPKRFTLPLREIWDMQERLPRRNGKRADLLLENPRFR
                     AGYDFLLLRESAGEQTDDLGEWWTRYQDVSDSERRSMIRALSEGEGRDKAAPRKRRRS
                     PRKRRS"
BASE COUNT          316 a          664 c          526 g          248 t
ORIGIN      
        1 gagaggagct ccccgacggg aacctccctc gaccggaccg agaacaataa aactgccccg
       61 aggcagcctc ccgcaccggt tgaacccgca agggtcgcgg cacgctcagc gtccaaagta
      121 atccgtttgt tctttagccc ctgcttgggg atattaccgg cctgccagtg ccggacgggt
      181 cgccaacaag aacaacaagg ctcggcagaa caacaagaac aaagcaccaa tcgacttaag
      241 gggagcctcg gctccccttg tgctttgtgc attggccagc ctactggcca ccctctcgtt
      301 caccatccca ccggccagtg ctagaattcg cctccattgc gcggggccga tcctgttccc
      361 gcctcatccg gtaaccagtc gccccatgct gaagaagctg ttccagtcca tccgctctcc
      421 cctgcgccgc aagcgcgccg tccgctcgac gccggaagtc ctcggcggca gccagcactc
      481 gctgcgccgc gagcagatca gccgcaacgc cgtcggcgtg gtcgagcgcc tgcagaaagc
      541 cggctatcag gcctatctgg tcggtggctg cgtgcgcgac ctgctgctcg ggcaccagcc
      601 caaggacttc gacgtcgcca ccagcgccac tcccgagcag gtgcgtgccg agttccgcaa
      661 cgcgcgggtg atcggccggc gcttcaagct ggtccacgtc catttcggcc gcgagatcat
      721 cgaggtggcc accttccgcg cccaccatcc gcagggcgag gacgacgagg acagtcatct
      781 cgcctcgcgc aacgagagcg ggcgcatcct gcgcgacaac gtctacggca atcaggaaga
      841 cgacgcccag cgccgcgact tcaccatcaa tgcgctgtac tacgacccgg ccagcgagca
      901 catcctcgac tacgcccacg gcgtgcacga catccgcaac cgcctggtgc gcctgatcgg
      961 cgatccggaa cagcgttacc tcgaggaccc ggtgcgcatg ctgcgtgcgg tgcgcttcgc
     1021 cgccaagctg gacttcgaca tcgagaagca cagcgccgcg ccgatccggc gcctggcccg
     1081 cctgctcaac gacattcccg cggcccgcct gttcgacgag atcctcaagc tgctgctctc
     1141 cggccagggc gaacgcacct tcgacctgct ctgcgaatac gacctgctcg ccccgctgtt
     1201 cccggccagc gccgccgcac tcaaggccga tccggagtat gccgggcgcc tgatgcgcaa
     1261 tgccctggcc aacaccgacg agcgcatccg ccagggcaag ccggtcaccc cggccttcct
     1321 gttcgccgcc ctgctgtggc cggccctgcc ggcacgcgcc gccgccctgc aggcgagcgg
     1381 catgccggcg ctgccggccc tgcaggaagc cgcccacgaa gtgatcagcg agcaatgccg
     1441 gcgtaccgcg attcccaagc gcttcaccct gccgctgcgg gaaatctggg acatgcagga
     1501 gcgcctgcca cgccgcaacg gcaagcgcgc cgacctgctg ctggagaacc cgcgcttccg
     1561 cgccggctac gacttcctgc tgctgcgcga aagcgccggc gaacagaccg acgacctcgg
     1621 cgagtggtgg acccgctatc aggacgtcag cgacagcgag cgccgcagca tgatccgtgc
     1681 cctcagcgaa ggcgaaggcc gcgacaaggc ggcgccgcgc aagcgccggc gcagcccgcg
     1741 caaacgccgc agcg
//
LOCUS       DATCUN010000193        17785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_8022, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000193 DATCUN010000000
VERSION     DATCUN010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 17785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17785
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8022"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VIO83_07685"
     CDS             <1..843
                     /locus_tag="VIO83_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8331192.1"
                     /translation="DSGALRILAGGEQPLADAELARPQSIRFATAEGERAHAFFYPPR
                     NAACAVPSNEKPPLVLFLHGGPTSACYPVFDPRIQFWTQRGFAVADLNYRGSSGFGRA
                     FRLRLAGEWGRIEVDDAVALVGHLGALGWVDARRAFIRGASAGGYTALCALAFHDVFR
                     GGASLYGVSDPLSLRRATHKFEGDYLDWLIGDPVRDAERYRARTPLLHAGRIAAPLIF
                     FQGGLDAVVVPQQTEAMVGALRARGVPVEYRLYAEERHGFRQAANLADALEREWQFYR
                     RLLA"
     gene            979..2139
                     /gene="ercA"
                     /locus_tag="VIO83_07690"
     CDS             979..2139
                     /gene="ercA"
                     /locus_tag="VIO83_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase-like regulatory protein
                     ErcA"
                     /protein_id="HEY8331193.1"
                     /translation="MSVISPLRKFVSPEIIFGAGSRHSVGNYAATFGARRVLVVSDPG
                     VQAAGWVGDVLASLDRQGIAHVLYTAVSPNPRVEEVMHGAELYRASGCDVIVAVGGGS
                     PMDCAKGIGIVAAHGRSILEFEGVDTIRVPSPPLILIPTTAGTSADVSQFVIISNQAE
                     RMKFSIVSKAVVPDVSLIDPETTVSMDPFLSACTGIDALVHAIEAFVSTGSGPLTDPH
                     ALEAMRLINGNLVQMIANPHDIALREKIMLGSMQAGLAFSNAILGAVHAMSHSLGGYL
                     DLPHGLCNAALVEHVVAFNFDAAPERFRVVAETFGIDCRGLTPPQVRGRLVDHLIAFK
                     RAVGFEESLKLHGVGSSDIPFLSQHAMQDPCILTNPRHSTQRDVEVVYGEAL"
     gene            2123..3901
                     /locus_tag="VIO83_07695"
     CDS             2123..3901
                     /locus_tag="VIO83_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NahK/ErcS family hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HEY8331194.1"
                     /translation="MAKPSDGRSDSGAQAGNRLSATPAAARPDVADLLGLGSHSARKS
                     YYPELSARLDELERERNRYKWLFEHAVHGIFQGRLNHGIRAANPALAHMLGYEDPQQV
                     LWSETDLAERLFVGGAAELARIRAVLRERHGLFGYETRLRRRDGSHIDVLMNLLLKPD
                     EEDVVEGFVADITERKQAQLRQQQLNVELEQRVAARTAELLEANRHLQQEIRQRERIQ
                     CELREARDAAEAANQSKDKYLAAASHDLLQPLNAARLLISTLRERALPASEANLVERT
                     HQALEGAEDLLADLLDIARLDSRAIRPDLAVYRLDELLAPLASEFQPVAEAAGLELRV
                     RIPRLAVNTDFRLLTRILRNFLSNACRYTGQGRVLLGCRRHGKQLLIEVWDTGRGIPA
                     DKLEEIFLEFNQLDAGRAAERKGVGLGLAIVERIARMLDYRIQVRSQPGRGSVFSIEV
                     PLADYVAPEPVARPRETLGNPLPGRRVLVVDNEPDIQQSMQALLAQWGCEVRVAGGLV
                     EARKQLSGWPAELLLVDYHLDQGANGCDLIQTLRSEAAAPLPAVMITAERSEQCRRQL
                     HEQGIPLLNKPVKPGKLRAALSQFLS"
     gene            4031..5086
                     /locus_tag="VIO83_07700"
     CDS             4031..5086
                     /locus_tag="VIO83_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8331195.1"
                     /translation="MLKRTLLVLGLTAGSALAHAEEVLRVYGGEGRLAPSLIEEFHKQ
                     TGIRVEYEAYAAAEEVLGDEAFGRRYDVVFPVHFQLPALVAAQRLQLLDPAKLANLKQ
                     IDPDLDTMLDGSSAQTRYAVPYQWGTVGLALNVPKVVEALGAVPEDSWSLLFDPRNNE
                     RLAKCGIGMLDARQETLSLLLNYKGRTLARSGPRQVQQAGEELAALRGKVATFGNRAY
                     IEQLASGQLCAAIAWSGHALKGADAGAPLRYVMPREGALMFIDTLAIPSNARNVAAAH
                     RFIDYLAGADVNTRNARETLFYPVIRGDAPAMNKLASERTDLVVASGQRRSFYYLESL
                     SPRQKAAVDAAWLQVKR"
     gene            complement(5076..5978)
                     /locus_tag="VIO83_07705"
     CDS             complement(5076..5978)
                     /locus_tag="VIO83_07705"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY8331196.1"
                     /translation="MLRHLPAPLRGVITSLLLGLNTLLWCWPLFALALLKLLPFSATQ
                     ALSRRLMGQVAARWADGNRAWMQWVQDTQWQVEGLEQLQVRGSCLVISNHQSWVDIFV
                     LQYHLNRRLPLLRFFLKKELLWIPVFGLCCWALDFPFMQRHSKAYLARHPERRDADRQ
                     TARQACARLRGVPVALFNFLEGTRFTEAKQAAQQSPYRHLLKPRAGGIALAIDAMGEQ
                     LEALVDVTIHYPDGRPRFRDLLCGRLQRVTVCFTQRPIPAEFLGRNYDQDAAYRLQFQ
                     QWVNGLWQDKDALLERLRRPPVSA"
     gene            6195..6680
                     /locus_tag="VIO83_07710"
     CDS             6195..6680
                     /locus_tag="VIO83_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8331197.1"
                     /translation="MQIAANKAVSIDYTLTNEGGEVIDSSAGGAPLVYLHGAGNIIVG
                     LEKALEGKQPGDELDVTIEPQEAYGDYSVELVAVLNRAMFEGVDELEVGMQFHASAPD
                     GGMQIVTIRDIEGEDVTVDGNHPLAGQSLNFKVKVIDVRDASAEEIAHGHVHGEGGHH
                     H"
     gene            6797..7288
                     /locus_tag="VIO83_07715"
     CDS             6797..7288
                     /locus_tag="VIO83_07715"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY8331198.1"
                     /translation="MSVFHDLNLKALDGQELPLAPFKGKVVLVVNVASKCGLTPQYAG
                     LEHLHQQYRERGFSVLGLPCNQFAGQEPGSEEEIRAFCSLNYGVSFPLSAKVEVNGHD
                     RHPLYLLLAGEGAEFPGDITWNFEKFLVGPDGRVLERFSPRVTPEDPQLIQAIEKALA
                     ALA"
     gene            complement(7311..8057)
                     /gene="cysZ"
                     /locus_tag="VIO83_07720"
     CDS             complement(7311..8057)
                     /gene="cysZ"
                     /locus_tag="VIO83_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HEY8331199.1"
                     /translation="MPTQALSGPGYLGAGLRLILSPGLRLFVLLPLTINLLLFIGLSV
                     LAFQQFGSWVDALMPGLPAWLSFLEYLLWPLFVVLVLLILFFGFTLLANLIAAPFNGF
                     LAEKVEAVVRGQDTSPPFSWGELLAMIPRTLGRELRKLGYFLPRALGLLVLSLIPGLN
                     LIAAPLWLLFGVWMMAVQYIDYPADNHKMGWDEMLAWLRAKRWQSLGFGAVTYAALLV
                     PGLNILLMPAAVAGATVFWVREDGATRLGR"
     gene            complement(8212..8553)
                     /locus_tag="VIO83_07725"
     CDS             complement(8212..8553)
                     /locus_tag="VIO83_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017476018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HopJ type III effector protein"
                     /protein_id="HEY8331200.1"
                     /translation="MTALTDFRAALQHDEHAFADTLAFITAHYDYQPQAFVNGGVENA
                     AGQNEGSCKLLGLALLEGLSDEETLRAFGEHYRSVLATPAGSDHGNIRALQTSGLAGV
                     RFAALPLTRKS"
     gene            complement(8550..8843)
                     /locus_tag="VIO83_07730"
     CDS             complement(8550..8843)
                     /locus_tag="VIO83_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HEY8331201.1"
                     /translation="MTEQQRLELEAAAFRRLLQHLGERSDVQNIELMNLAGFCRNCLG
                     KWYKAAADERGIELSAEDARDTIYGMPYAEWKAQHQKEATPEQLAAFNQASKP"
     gene            complement(8944..9315)
                     /gene="folX"
                     /locus_tag="VIO83_07735"
     CDS             complement(8944..9315)
                     /gene="folX"
                     /locus_tag="VIO83_07735"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HEY8331202.1"
                     /translation="MPRLEPGMARIRVKDLRLRTYIGIKEEEILNKQDVLINLTILYP
                     AQDAVQGNDIDQALNYRTITKAVIHHVEENRFALLERLTQEILDLVMANPAVRYAEVE
                     VDKLHALRFAESVSITLAGHR"
     gene            complement(9316..9885)
                     /gene="folE"
                     /locus_tag="VIO83_07740"
     CDS             complement(9316..9885)
                     /gene="folE"
                     /locus_tag="VIO83_07740"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY8331203.1"
                     /translation="MSDQLSQITQHYREILKGLGEDTEREGLLDTPKRAAKAMQYLCR
                     GYEQSLDEIVNGALFASDNDEMVIVRDIELYSLCEHHLLPFIGKAHVAYIPTGKVLGL
                     SKVARIVDMFARRLQIQENLTRQIAEAVQQVTNAAGVAVVIEAQHMCMMMRGVEKQNS
                     VMTSSVMLGAFRQSTNTRQEFLQLIGRSK"
     gene            complement(9930..10640)
                     /gene="folM"
                     /locus_tag="VIO83_07745"
     CDS             complement(9930..10640)
                     /gene="folM"
                     /locus_tag="VIO83_07745"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HEY8331204.1"
                     /translation="MSQSPAPILITGASQRVGLHCARRLLDAGQPLIVSYRTERPALD
                     ELRQRGALALPADFSSEAGILAFIDQLKQHTDRLRAIVHNASDWLREEPGHEAEAFTR
                     MVNVHMLAPYLINLRCRELLERCELADIVHITDDVARRGSANRVAYCASKAGLENLTL
                     SFAAQFAPRIKVNAIAPALVMFHEHDDAHYREKTLAKSALGIEPGAEVIYQSLRYLLD
                     NPYVTGTSLCVNGGRHLK"
     gene            complement(10933..11373)
                     /locus_tag="VIO83_07750"
     CDS             complement(10933..11373)
                     /locus_tag="VIO83_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEY8331205.1"
                     /translation="MRHPTVWPLTLYYDGNCPLCAREIRALRRRASPQRLQLVDIDAA
                     DFDPAPLGFSRSQLQQRLHARFADGQWVSGLDATFWSWCAAGLGRWAAPLRWPLLRPL
                     LELGYRLFCRLRPYLHWLPHTDGAARCRAGACELPDKTGNAGRD"
     gene            11470..12423
                     /locus_tag="VIO83_07755"
     CDS             11470..12423
                     /locus_tag="VIO83_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8331206.1"
                     /translation="MPEFSVASVSVPAVPREEELYPIREVSRLTGVNPVTLRAWERRY
                     GLIQPTRTDSGHRLYSLADIDAVRSILAWIERGVAVSKVGKILARSSTAKAPSAPAYD
                     DVAAGEWGEWQAQLRRAVAAFDEVGLERLYGQVFSSYPLAVVFQDVLLPVWQELLLRQ
                     DGFGQTSEWLFLDTFLRGRALQRLQLSRGQSEERVLLAALPGHGHELELLVTGLLLAG
                     SDLAVSLLGIGQPFDELPLVCEKIQPQALVLFSNRPPEAELPRQLGRLVLALDCPLAL
                     AGEVAELAEESLQGTPIACLGSEGRLMPRRLRQFLAGHLDT"
     gene            complement(12791..13246)
                     /locus_tag="VIO83_07760"
     CDS             complement(12791..13246)
                     /locus_tag="VIO83_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8331207.1"
                     /translation="MINAKLLQLVIDASNDGIVVAEQEGDDNILIYANRAFERLTGYS
                     VDDILYQDCRFLQAGDRTQTGLDAIRQAVREHRPCRQVIRNYRKDGTPFWNELSITPV
                     LNEADQLTYYIGIQKDVTEQVEAEQRVRELEAELAAVKAERDALRAGKA"
     gene            13367..13819
                     /locus_tag="VIO83_07765"
     CDS             13367..13819
                     /locus_tag="VIO83_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEY8331208.1"
                     /translation="MLEVAILSGSAFGAAEEVARHAEQQLTAAGLAACHLPDIGLDAL
                     LALDPQALLVVTSTTGMGEVPDDLQALLDEIDAWQPDWRGRPVAVIGLGDSCYGENFC
                     AAALQAHAQLLKLGMRDVQSQLQLDASETVTPAEDAEPWLARLAANLR"
     gene            14015..15037
                     /locus_tag="VIO83_07770"
     CDS             14015..15037
                     /locus_tag="VIO83_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEY8331209.1"
                     /translation="MFATMEAVRVEELHLASDPQTGLRAIVAIHNTHLGPALGGCRYL
                     AYADEDSAARDAVRLARGMSYKAALAGLALGGGKAVIIRPPQPSSREALFEAFGRFVD
                     KLGGRYITAVDSGTSGADMDCIARHTSHVTSTTAGGDPSPHTALGVFAGIRATALARL
                     GSDDLEGLRIAVQGLGHVGYALAGHLAAAGAELLVSDIDESRVQLAVERFGAQPVSSE
                     ALLTVPCDILAPCGLGGAINPQTVGQLRCAAVAGAANNQLDSLEMGDELAARGILYAP
                     DYVINSGGLIFVALQHQGRSPAEITAHLAQIGSRLSEIYAEALAEQRSPARVADSLAE
                     RLIYGC"
     gene            15102..16199
                     /gene="pdhA"
                     /locus_tag="VIO83_07775"
     CDS             15102..16199
                     /gene="pdhA"
                     /locus_tag="VIO83_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEY8331210.1"
                     /translation="MPHSRIDLPYTRYLDADGRPLEPLPDWVEDEARVVGLYRAMLRT
                     RLFDQKVIALQRTGRIGTYASSLGQEAIGTAIGSLLRDDDVFLPYYRDSAAQLLRGVS
                     MTEMLLYWGGDERGSAYAGPVARGDFPLCVPIATQALHACGVASAFKIRGEARVALTT
                     CGDGATSKGDFLEALNVAGAWQLPVVFVVNNNQWAISVPRRIQCGAPTLAQKAIGAGF
                     AGEQVDGNDVLAVAERLRQAIDHARHGKGPTLLECITYRLCDHTTADDATRYRSAEEV
                     NQAWHEEPIKRLQNFLVARGWWDEQREQALVAETQAELQQAIDAYEATPAQDLQEVFR
                     HVYADWPAALAEQREQFAERVARRTQGADHE"
     gene            16192..17178
                     /locus_tag="VIO83_07780"
     CDS             16192..17178
                     /locus_tag="VIO83_07780"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019647693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY8331211.1"
                     /translation="MSEKKYTLLEAVNLALHRAMAEDANVVVLGEDIGVNGGVFRATL
                     GLREAFGFKRVIDTPLAENMIAGLSIGMASQGLKPVMEIQFLGFIYAAMEQLVSHASR
                     LRNRTRGRLSCPLVLRSPMGAGIRAPEHHSEATEALFAHIPGLRVVIPSSPARAYGLL
                     LAAIDDPDPVIFLEPTRLYRMNPQPLVDDGRRLPLDSCFTLREGSDITLISWGASVHE
                     TLQAAGALAERGVSAEVIDVASVKPLDLDTLEASVRKTGRCVIVQEAPKSGGVGAEIA
                     ASLYERVLLDLQAPIQRVAAPDIPPPLYRLEPYYLPDVGDILAACDAVLEYA"
     gene            17325..>17785
                     /locus_tag="VIO83_07785"
     CDS             17325..>17785
                     /locus_tag="VIO83_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY8331212.1"
                     /translation="MKHFKLPDLGEGLQEAEIVEWHVKPGDSVKADQLLVSVETAKAI
                     VDIPAPYDGVVLKTYGVDGDIIHVGEPLLAYEGEGEDSGTVVGKLEAGEGGEGDSFFV
                     GAAPSTREHLAPRATPAVRQLARQLGVELGGLTGSGADGLITRADVEAVAE"
BASE COUNT         2751 a         5979 c         6223 g         2832 t
ORIGIN      
        1 gattcggggg cgctgcgcat cctcgccggc ggcgagcagc cgctggccga cgccgaactg
       61 gcgcgcccgc agtcgatccg cttcgccacg gcggaggggg agcgcgccca cgccttcttc
      121 tacccgccgc gcaacgccgc ctgcgcggtg cccagcaacg agaagccgcc gctggtgctg
      181 ttcctgcacg gcggaccgac ctcggcctgc tatccggtgt tcgacccgcg catccagttc
      241 tggacccagc gcggctttgc cgtggccgac ctcaactacc gcggctccag cggtttcggc
      301 cgcgctttcc gtctgcgtct ggccggcgag tgggggcgca tcgaggtgga cgacgccgtc
      361 gccctggtcg gtcatctcgg cgcgctgggc tgggtggatg cccggcgcgc ctttatccgc
      421 ggcgccagtg ccggcggcta taccgcgctg tgcgcgctgg ccttccacga cgtcttccgc
      481 ggcggcgcca gcctgtacgg ggtcagcgat cccttgagcc tgcgccgcgc cacccacaag
      541 ttcgagggcg actacctgga ctggctgatc ggcgaccccg tgcgcgatgc cgaacgctac
      601 cgggcgcgca cgccgctgtt gcacgccggg cgcatcgccg cgccgctgat cttcttccag
      661 ggcggcctgg atgcggtggt ggtgccgcag cagaccgagg cgatggtcgg cgccctgcgc
      721 gcgcgcggcg tgccggtgga ataccggctg tatgccgagg agcgccacgg cttccgccag
      781 gccgccaacc tggccgatgc cctcgagcgc gagtggcagt tctaccggcg cttgctcgct
      841 tagcgccggg cctggcggac ggaagccgct accgtagagc ggctgtgcca ccttgggtgg
      901 attgcaaaga ccgactaatg cagtaggctg tcgtttagca atagttcata acaacaacga
      961 tgttcgtcag gtccctgcat gtctgtcatc agcccgcttc gcaagttcgt cagcccggaa
     1021 atcatcttcg gcgccggctc ccgccactcg gtcggcaact acgccgcgac cttcggtgcg
     1081 cgccgggtgc tggtggtgtc cgatcccggc gtgcaggcgg ccggctgggt cggcgacgtg
     1141 ctggccagcc tggatcgcca gggcatcgcc cacgtgctgt acaccgccgt gtcgcccaac
     1201 ccgcgggtcg aggaggtgat gcacggcgcc gagctgtacc gcgccagcgg ctgcgacgtg
     1261 atagtcgcgg tcggcggcgg cagcccgatg gactgcgcca agggcatcgg catcgtcgcc
     1321 gcccacgggc gcagcatcct cgagttcgag ggcgtcgata ccatccgcgt gcccagcccg
     1381 ccgctgatcc tgatccccac caccgccggc acttcggccg acgtgtcgca gttcgtgatc
     1441 atctccaacc aggccgagcg gatgaagttc tccatcgtca gcaaggcggt ggtgccggac
     1501 gtgtcgctga tcgatccgga aaccaccgtc agcatggatc cgttcctctc cgcgtgcacc
     1561 ggcatcgacg ccctggtgca cgccatcgag gccttcgtct ccaccggcag cgggccgctc
     1621 accgaccccc acgcgctgga ggcgatgcgg ctgatcaacg gcaacctggt gcagatgatc
     1681 gccaatccgc acgacatcgc cctgcgcgag aagatcatgc tcggcagcat gcaggccggt
     1741 ctggccttct ccaacgccat tctcggcgcg gtgcacgcca tgtcgcacag cctcggcggc
     1801 tacctcgacc tgccgcacgg cctgtgcaac gccgcgctgg tcgagcacgt ggtggcgttc
     1861 aacttcgacg ccgcgccgga gcgctttcgc gtggtcgccg agaccttcgg catcgactgc
     1921 cgcggcctga ccccgccgca ggtgcgtggc cggctggtcg accacctgat cgccttcaag
     1981 cgcgcggtcg gcttcgagga gtccctcaag ctgcacggcg tgggcagctc ggacattccc
     2041 ttcctgtcgc agcacgcgat gcaggaccca tgcatcctga ccaaccccag gcactcgacc
     2101 cagcgcgacg tcgaggtggt gtatggcgaa gccctctgac ggtcgctccg acagcggcgc
     2161 tcaggcgggc aatcggttgt ccgccacgcc cgcggcggcc cgtccggacg ttgccgacct
     2221 gctcggtctc ggcagccact cggcgcgcaa gagctactac ccggagctgt cggcgcgcct
     2281 cgatgagctg gagcgcgaac gcaaccgcta caagtggctg ttcgagcacg cggtgcacgg
     2341 catcttccag gggcgcctga accacggcat ccgcgccgcc aatccggcgt tggcgcacat
     2401 gcttggctac gaggatccgc agcaggtgct gtggtcggag accgacctgg ccgagcggct
     2461 gttcgtcggc ggcgccgccg agctggcgcg cattcgcgcg gtgctgcgcg agcgccacgg
     2521 cctgttcggc tacgagacgc gcctgcgccg gcgcgacggc agccacatcg acgtgctgat
     2581 gaacctgctg ctcaagcccg acgaggagga tgtggtcgag ggcttcgtcg ccgacatcac
     2641 cgagcgcaag caggcccagc tgcgccagca gcagctcaac gtcgagctgg agcagcgcgt
     2701 cgccgcgcgc accgccgagc tgctcgaggc caaccgccac ctgcagcagg agatccgcca
     2761 gcgcgagcgc atccagtgcg agttgcgcga ggcgcgcgac gccgccgagg cggccaacca
     2821 gagcaaggac aagtacctgg ccgcggccag tcacgacctg ctgcagccgc tcaacgccgc
     2881 gcgcctgctg atctccaccc tgcgcgagcg cgccctgccg gccagcgagg ccaacctggt
     2941 ggagcgtacc catcaggccc tggaaggcgc cgaggacctg ctcgccgacc tgctcgacat
     3001 cgcccgcctg gacagccggg cgatccgccc ggacctggcg gtgtatcgcc tcgacgagct
     3061 gctggcgccg ctggcctcgg agttccagcc ggtggccgag gcggccggcc tcgagctgcg
     3121 cgtgcgcatc ccgcgcctgg cggtgaacac cgatttccgc ctgctcaccc gcatcctgcg
     3181 caacttcctc agcaacgcct gccgctacac cgggcagggc cgcgtgctgc tcggttgccg
     3241 ccgccacggc aagcagctgc tgatcgaggt atgggacacc gggcgcggca tcccggccga
     3301 caagctggag gagatcttcc tcgagttcaa ccagctcgac gccggtcgcg ccgccgagcg
     3361 caagggcgtc ggcctcggcc tggctatcgt cgagcgcatc gcgcgcatgc tcgactaccg
     3421 catccaggtg cgctcgcagc cgggacgcgg ctcggtgttc agcatcgagg tgccgctggc
     3481 cgactacgtg gcgccggagc cggtcgcgcg cccgcgcgag acgctgggca atccgctgcc
     3541 cgggcgccgc gtgctggtgg tcgacaacga gccggacatc cagcagagca tgcaggcgct
     3601 gctggcccag tggggctgcg aggtgcgcgt ggccgggggg ctggtggagg cgcgcaagca
     3661 gctctccggc tggccggccg agctgctgct ggtggactat cacctcgacc agggcgccaa
     3721 cggctgcgat ctaatccaga cgctgcgcag cgaggcggcc gcgccgctgc cggcggtgat
     3781 gatcaccgcc gagcgcagcg agcagtgccg ccgccagctg cacgaacagg gcattccgct
     3841 gctcaacaaa ccggtcaagc cgggcaagct gcgcgccgcg ctcagtcagt ttctttcctg
     3901 aaatgtgaag caaatcacct ttggcgcaat gagttgcgac aaaggtcgta tagcccgttt
     3961 aaatcctgcg cgcatagccg ctgccatggg tgcggcttgc ccatccaata agaacaagga
     4021 gtaacgggtc atgttgaagc ggacgctgct ggtgctgggg ctgactgcgg ggagcgcgct
     4081 ggcgcacgcc gaagaagtgc tgcgggtgta cggcggggag gggcgactgg cgcccagcct
     4141 gatcgaggag ttccacaagc agacgggaat ccgcgtggag tacgaggcct acgccgcggc
     4201 ggaggaggtg ctgggcgacg aggctttcgg ccggcgttac gacgtggtct tccccgtgca
     4261 cttccagctg ccggcgctgg tcgccgcgca gcgcctgcaa ctgctggatc cggccaagct
     4321 ggccaacctg aaacagatcg atcccgacct ggacaccatg ctcgacggct cctcggcgca
     4381 gacccggtat gccgtaccct atcagtgggg gaccgtcggc ctggcgctga acgtgcccaa
     4441 ggtggtcgaa gcgctgggcg cggtgcccga ggacagctgg agtctgctgt tcgatccccg
     4501 caacaacgag cgcctggcca agtgcggcat cggcatgctg gatgcgcgcc aggaaaccct
     4561 ctcgctgctg ctcaactaca aggggcgcac gctggcccgc agcggcccgc ggcaggtcca
     4621 gcaggccggc gaggagctgg ccgcgctgcg cggcaaggtc gccaccttcg gcaaccgcgc
     4681 gtacatcgag cagctggcca gcggccagtt gtgcgcggcc atcgcctgga gcgggcatgc
     4741 gctgaagggg gccgacgccg gggcgccgct gcgttacgtc atgccgcgcg agggcgcgct
     4801 gatgttcatc gacaccctgg cgattcccag caacgcccgg aacgtcgcgg cggcgcatcg
     4861 cttcatcgac tacctggccg gtgccgacgt gaacacccgc aatgcccgcg agacgctgtt
     4921 ctacccggtc atccgcggcg atgcgccggc gatgaacaag ctggccagcg agcggacgga
     4981 cctggtggtc gccagcggcc agcgacgcag cttctactac ctggagtccc tgagtccgcg
     5041 gcagaaggcc gcggtggacg ccgcctggct gcaggtcaag cgctgaccgg cggccgccgc
     5101 agacgctcga gcagggcatc cttgtcctgc cacaggccgt tgacccactg ctggaactgc
     5161 aggcggtagg ccgcgtcctg gtcatagttg cggccgagga actcggcggg gatcggccgc
     5221 tgggtgaagc acaccgtcac ccgttgcagc cgcccgcaga gcaggtcgcg aaagcgcggg
     5281 cggccgtcgg ggtagtggat ggtcacgtcg accagcgctt ccagttgctc gcccatcgcg
     5341 tcgatggcca gggcgatgcc gccggcgcgc ggcttgagca ggtggcggta gggcgactgc
     5401 tgcgccgcct gcttggcctc ggtgaagcgc gtgccctcga ggaagttgaa cagcgccacc
     5461 ggcacgccgc gcagacgcgc gcaggcctgg cgcgcggtct gccggtcggc gtcgcggcgc
     5521 tccgggtggc gcgccaggta ggccttggag tggcgctgca tgaacgggaa gtccagcgcc
     5581 cagcagcaca ggccgaacac cggaatccac agcagctcct tcttcaggaa gaaccgcagc
     5641 agcggcaggc ggcggttgag gtggtactgc aggacgaaga tgtcgaccca gctctggtgg
     5701 ttgctgatca ccaggcagga gccgcgcacc tgcagctgct cgagtccctc gacctgccac
     5761 tgggtatcct gcacccactg catccacgcc cggttgccgt cggcccagcg ggcggcgacc
     5821 tggcccatca ggcgccggct cagcgcctgg gtggcgctga atggcaacag cttgagcagc
     5881 gccagggcga acagcggcca gcaccacagc agggtgttga gccccagcag caggctggtg
     5941 atgacaccgc gcaacggcgc gggcaggtgt ctgagcatcg cgggttctcg aacggtgggc
     6001 cggcagggcc ggatgatact gcagccgtgg ccgtggcata aaatcgctgg acttggccgt
     6061 tgttggcgga caatcgtggc gctgcgcacg cggccgcatc cgcctgtccc gcagcgggtc
     6121 gctttcaacc cggcggcaac gtagaatcag cgttcgcacc tatccatatc caaaatattc
     6181 aaaggtttca aaccatgcag atcgcggcca acaaggcggt atccatcgac tacaccctga
     6241 ccaacgaagg cggtgaagtc atcgacagct ccgccggcgg tgcaccgctg gtatacctgc
     6301 atggcgccgg caacatcatc gtcggtctgg aaaaggccct cgaaggcaag cagcccggcg
     6361 acgagctgga cgtcaccatc gagccgcagg aagcctacgg cgactacagc gtcgagctgg
     6421 tggccgtgct caaccgcgcc atgttcgaag gcgtcgacga gctggaagtc ggcatgcagt
     6481 tccacgcctc cgctccggac ggcggcatgc agatcgtcac catccgcgac atcgaaggcg
     6541 aggacgtcac cgtcgacggc aaccacccgc tggctggcca gagcctgaac ttcaaggtca
     6601 aggtgatcga cgtgcgcgac gcctctgccg aggaaatcgc ccacggtcac gtgcatggcg
     6661 aaggtggtca tcaccactaa gcccggcaag cagatgtcca tctgctgcta agctgaagta
     6721 caggaaaggc gctattgcga tccgtgatag tgcctttttt ttggatcgtt gttccggatt
     6781 tgcatggagc cttgtgatga gcgttttcca cgacctgaat ctgaaggccc tggacggcca
     6841 ggagctgccc ctggcgccgt tcaagggcaa ggtggtgctg gtggtcaatg tcgcctcgaa
     6901 gtgcggcctg actccgcaat acgccgggct ggagcatctc catcagcagt accgcgagcg
     6961 cggcttcagc gtgctcggcc tgccgtgcaa ccagttcgcc gggcaggagc cgggcagcga
     7021 ggaggaaatc cgcgcgttct gcagcctcaa ctacggggtc agcttcccgc tgagcgccaa
     7081 ggtcgaggtc aacggccatg accgccatcc gctgtacctg ctgctggcgg gcgagggcgc
     7141 ggagtttccc ggcgacatca cctggaactt cgagaagttc ctggtcggcc cggacggtcg
     7201 ggtgctcgag cgtttctcgc cgcgcgtcac gccggaagat ccgcagctga tccaggcgat
     7261 cgagaaggcc ctggcggcgc tggcctgagc cgccgccccc cgccgttggc tcagcgcccc
     7321 agtcgcgtgg cgccgtcctc gcgtacccag aacaccgtcg ctccggccac tgcggccggc
     7381 atcaacagga tgttcagccc cggcaccagc agcgcggcgt aggtgacggc gccgaagccc
     7441 aggctctgcc agcgcttggc gcgcagccag gcgagcatct cgtcccagcc catcttgtgg
     7501 ttgtccgccg ggtagtcgat gtactgcacc gccatcatcc atacaccgaa cagcagccac
     7561 agcggcgcgg cgatcaggtt cagccccgga atcagcgaca gcaccagcag ccccagcgcc
     7621 cgcggcagga agtagccgag cttgcgcagt tcgcggccga gggtgcgcgg gatcatcgcc
     7681 agcagctcgc cccagctgaa cggcggcgag gtgtcctggc cgcgcaccac ggcctcgacc
     7741 ttctcggcga ggaagccgtt gaacggtgcg gcgatcaggt tggccagcag ggtgaagccg
     7801 aagaacagga tcagcagcac cagcaccacg aacagcggcc acagcaggta ttcgaggaag
     7861 ctcagccagg cgggcaggcc gggcatcagg gcgtcgaccc aggagccgaa ctgctggaac
     7921 gccagcacgc tcaggccgat gaacagcagc aggttgatgg tcagcggcag caggacgaac
     7981 aggcgcaggc cggggctgag gatcaggcgc aggccggcgc cgagatagcc ggggccggag
     8041 agggcttggg tgggcatggg taactccgtg gggaacacgg ggccaactta acacggggcg
     8101 tgccgcaggc cgtaacccac cagcgatgcc gccaggcatc gcgagcaccg gcctcgcggc
     8161 cggctggtgg gttacgccgc tctgcggcta acccacccta cggggggtat ctcagctctt
     8221 gcgcgtcagc ggcagcgcgg cgaagcgcac gccggccagg ccgctggtct gcagggcgcg
     8281 gatgttgccg tggtcgctgc ctgcgggggt ggccagcacg ctgcggtagt gttcgccgaa
     8341 ggcgcgcagg gtttcctcgt cgctcagccc ttcgagcagg gccaggccga gcagcttgca
     8401 cgagccctcg ttctgcccgg cggcgttctc cacgccgccg ttgacgaagg cctgcggctg
     8461 gtagtcgtag tgggcggtga tgaacgccag ggtgtcggcg aaggcgtgct cgtcgtgctg
     8521 cagggcggcg cggaagtcgg tcagtgcggt catggcttgc tcgcttggtt gaaggcggcc
     8581 agttgctcgg gcgtggcctc tttctggtgt tgggctttcc actcggcgta gggcatgccg
     8641 tagatggtgt cgcgggcgtc ctcggcggac agctcgatgc cgcgctcgtc ggcggcggcc
     8701 ttgtaccact tgcccaggca gttgcggcag aagccggcga ggttcatcag ctcgatgttc
     8761 tgcacgtcgc tgcgctcacc cagatgctgg agcaggcggc ggaaggcggc ggcttcgagt
     8821 tcgaggcgtt gttgttcggt catggcggac tctgcagggg gccggttggg cgtcgcgggc
     8881 gcggcgcgtt cgcggcgatc ataagagccg cggcggcgcc ggacaatgcc cggcgggcag
     8941 ccctcagcga tgcccggcca gggtgatcga caccgactcg gcaaagcgca gggcgtgcag
     9001 cttgtcgacc tcgacctcgg cgtagcgcac cgccgggttg gccatcacca ggtcgaggat
     9061 ctcctgggtc agccgttcga gcagggcgaa gcggttttcc tcgacatggt gaatcaccgc
     9121 cttggtaatg gtgcggtagt tcagcgcctg gtcgatgtcg ttgccctgca ccgcgtcctg
     9181 cgccgggtag aggatggtca ggttgatcag cacatcctgc ttgttgagga tttcctcctc
     9241 cttgataccg atgtaggtgc gcaggcgcag atccttgacg cggatgcgcg ccatgccggg
     9301 ctccagtcga ggcatttact tgctccgtcc gatcagttgc aggaattcct ggcgggtgtt
     9361 ggtcgactgg cggaaggcgc cgagcatcac cgaggaggtc atcaccgagt tctgcttttc
     9421 caccccgcgc atcatcatgc acatgtgctg cgcctcgatc accacggcca cgccggcggc
     9481 gttggtcacc tgctggacgg cctcggcgat ctgccgggtg aggttttcct ggatctgcag
     9541 gcggcgggcg aacatgtcga cgatgcgcgc caccttggac aggccgagca ccttgccggt
     9601 ggggatgtag gcgacgtggg ccttgccgat gaacggcagc aggtggtgtt cgcacagcga
     9661 gtacagctcg atgtcgcgga cgatcaccat ctcgtcgttg tcggaggcga acagcgcgcc
     9721 gttgacgatc tcgtccagcg actgctcgta gccgcggcac aggtactgca tggccttggc
     9781 ggcccgcttg ggggtgtcca gcaggccttc acgctcggta tcctcgccga ggcctttgag
     9841 gatttcgcgg taatgctggg tgatttgcga cagttgatcg ctcatgttcg attcctcgtg
     9901 gcggccgctt ggggctcact gtagcgggct cacttcaggt ggcggccgcc gttgacgcac
     9961 aggctggtgc cggtgacgta ggggttgtcc agaagatagc gcaggctctg gtagatgacc
    10021 tccgcgcccg gctcgatgcc gagggccgac ttggccaggg ttttctcgcg gtagtgcgcg
    10081 tcgtcgtgtt cgtgaaacat caccagcgcc ggggcgatgg cgttgacctt gatccgcggg
    10141 gcgaactgcg cggcgaacga cagggtcagg ttctccagcc cggccttgct ggcgcagtag
    10201 gccacgcggt tggcgctgcc gcggcgggcg acgtcgtcgg tgatatgcac gatatcggcc
    10261 aactcgcagc gctcgagcaa ttcgcggcaa cgcaggttga tcaggtaggg cgccagcata
    10321 tgcacgttga ccatccgggt gaaggcctcg gcctcgtgtc cgggctcctc gcgcagccag
    10381 tcggaggcgt tgtgcacgat ggcgcgcagg cggtcggtgt gctgcttgag ctggtcgatg
    10441 aaggcgagga tgccggcttc gctggaaaag tcggccggca gggccagcgc gccgcgctgg
    10501 cgcagctcgt cgagcgccgg acgctccgtg cggtagctga cgatcagcgg ctggccggcg
    10561 tcgagcaggc gccgcgcaca gtgcaacccg acccgctggc tggcgccggt gatcaggatg
    10621 ggggcgggtg actggctcat gacggcctct tggattcaag gatggttcag cggcacagcc
    10681 gccgggtacc tgacaagtgc gggacggagc accgactgaa ccaggagcaa agttatatca
    10741 gtattcgaag ttgtacaacc ctgcgagccg tgtgggtagt cggatgttgg ccggtgtctg
    10801 gccgaaaaag gcccttaaag cgccggtttc cccggctgtg cgggcttctt gaactggcat
    10861 tttattgtac tgtatttttt attttgtaca tgtttttggc agcttttccg gccattgcaa
    10921 cgctggcggg cctcagtccc tgccggcgtt gccggttttg tctggcaact cgcaggctcc
    10981 tgcgcggcag cgcgccgcgc cgtccgtgtg gggcagccag tgcaggtagg ggcgcaggcg
    11041 gcagaacagt cgatagccga gctccagcag cggacgcaac agcggccagc gcaagggcgc
    11101 ggcccagcga ccgaggccgg cggcgcacca gctccagaag gtggcgtcga ggccgctgac
    11161 ccactggcca tcggcgaagc gcgcgtgcag gcgctgctgc agctggctgc ggctgaagcc
    11221 gagcggggcg ggatcgaagt cggcggcgtc gatgtccacc agttgcaggc gctgtggcga
    11281 ggcacgccgg cgcagggcgc ggatctcgcg ggcgcacagc gggcagttgc cgtcgtagta
    11341 gagcgtcagt ggccagacgg tggggtggcg catggcgagc gtccttctgt gtataggtct
    11401 tgtacaattt aagccggcaa tggcctgggt tacaatcgcc actcacatcc gccgtgcggt
    11461 gcccatgcca tgcccgaatt ctccgtcgcc tccgtcagcg ttcccgccgt accccgcgag
    11521 gaagagctgt atccgatccg tgaggtgtcg cgcctgaccg gcgtcaaccc ggtcaccctg
    11581 cgggcctggg agcggcgcta cgggctgatc cagccgaccc gcaccgacag cggccatcgc
    11641 ctgtattcgc tggccgacat cgacgcggtg cgcagcatcc tggcctggat cgagcgcggc
    11701 gtggcggtca gcaaggtcgg caagatcctc gcccgcagca gcacggccaa ggcgcccagc
    11761 gcgccggcct acgacgacgt ggccgccggt gagtggggcg agtggcaggc gcagctgcgc
    11821 cgcgccgtgg cggccttcga cgaggtgggc ctggagcgcc tctacggcca ggtgttctcc
    11881 agctatccgc tggcggtggt gttccaggac gtgctgctgc cggtgtggca ggagctgctg
    11941 ctgcgccagg acggtttcgg ccagaccagc gagtggctgt tcctcgacac cttcctgcgc
    12001 ggccgcgccc tgcagcgcct gcagctgagt cgcgggcaga gcgaggagcg cgtgctgctg
    12061 gccgcgctgc ccggccatgg ccacgagctg gagctgctgg tgaccggtct gctgctggcc
    12121 ggcagcgacc tggcggtgag cctgctcggc attggccagc cgttcgacga gctgccgctg
    12181 gtgtgcgaga agatccagcc gcaggcgctg gtgctgttct ccaatcgtcc gccggaagcc
    12241 gagctgccgc gccagctcgg gcgcctggtg ctggcgctgg actgcccgct ggcgctggcc
    12301 ggcgaggtgg ccgagctggc cgaggagtcc ctgcagggca cgccgatcgc ctgcctgggc
    12361 agcgaagggc ggctgatgcc gcggcgtctg cgccagttcc tcgccggtca tctggatacc
    12421 tgacgggcac cttcatcggc ccggctgccc gggtcgatat cccaaaattc cacacacgtc
    12481 attcgtcgcc cgggcatggc tcgggtggcg ccttgggctg tcggcgaaaa aatactgtgc
    12541 gcgctgtaca ggaatgcttg ctgtgtacgg caatttatta tacaaaagct atacagaagt
    12601 gcgcgctgta cagtccatcc cctgcaagcc tgtacgccgc accctgcctg cgcactccgg
    12661 cagccgccga tcccggattt tttggctgcc ggagtttttt attttgcgga ttgcgatgtg
    12721 gctgggcgcg acccgcgcgt ctgcgccagt acgccggcgg cagagcgcgg actgaagtcg
    12781 cccggcgggc tcaggccttg ccggcgcgca gggcgtcccg ctcggccttc accgcggcca
    12841 gctcggcttc cagctcgcgc acgcgctgtt cggcctcgac ctgctcggtg acatccttct
    12901 ggatgccgat gtagtaggtc agctggtcgg cctcgttgag caccggggtg atcgacagtt
    12961 cgttccagaa cggcgtgcca tccttgcggt agttgcggat cacctggcgg cacgggcgat
    13021 gctcgcgcac cgcctggcgg atggcgtcca gcccggtctg ggtacggtcg ccggcctgca
    13081 ggaagcggca gtcctgatag aggatgtcgt cgacgctgta gccggtcagg cgctcgaagg
    13141 cccggttggc atagatgagt atgttgtcgt cgccttcctg ttcggccacc acgatgccgt
    13201 cgttggaggc gtcgattacc agttgcagca gcttggcgtt gatcatcggg actgtcctgt
    13261 aggtgatggc ggcattctaa tccatccctg tgcgcttgcc atggcggcgc agcggttgcg
    13321 cattggcgca taatggcggc tttcccttgc tgcccgagga gcctccatgc tcgaagtcgc
    13381 cattctttca ggttctgcct tcggtgccgc cgaagaggtg gcccgccatg ccgagcagca
    13441 gctgacggcc gccggcctgg ccgcgtgcca tctgccggat atcggcctcg acgcgctgtt
    13501 ggcgctggac ccgcaggcgc tgctggtggt gacctcgacc accggcatgg gcgaggtgcc
    13561 ggacgacctg caggcgctgc tcgacgagat cgacgcctgg cagccggact ggcgcggccg
    13621 gccggtggcg gtgatcggcc tgggcgactc ctgctacggc gagaacttct gtgccgccgc
    13681 cctgcaggcc catgcgcagc tgctgaagct gggcatgcgt gatgtgcagt cgcaactgca
    13741 actggatgcc agcgagacgg tgaccccggc cgaggacgcc gagccgtggc tggcgcgcct
    13801 ggcggccaac ctgcgctgag agcctgatcg ccgggcagcg gggcgagcgc tgcctatact
    13861 gagacgaaag aagcgtcgcg ttggccgtac gggccacgtg gatcgcgacg cgggcggatg
    13921 tgtgtcgggt cggctctcca ctcggcacag acaccgcctg atacgcgatc ctgccgacga
    13981 cgccgaactc atcgaccagg caggagttcg tctcatgttc gccaccatgg aagccgtgcg
    14041 cgtcgaggag ctgcatctgg ccagcgaccc gcagaccggc ttgcgcgcca tcgtcgccat
    14101 ccacaacacc catctcggcc cggcgctcgg cggctgccgc tatctggcct atgccgacga
    14161 ggacagcgcc gcgcgcgatg ccgtgcgtct ggcccgcggc atgagctaca aggccgcgct
    14221 ggccggcctg gccctgggcg gcggcaaggc ggtgatcatc cgcccgccgc agccgagcag
    14281 ccgcgaggcg ctgttcgagg ccttcggccg cttcgtcgac aagctcggcg ggcgctacat
    14341 cacggcggtg gacagcggca cctcgggtgc cgatatggac tgcatcgccc ggcacaccag
    14401 ccatgtcacc agcaccacgg cgggtggcga tccgtcgccg cacaccgccc tcggcgtgtt
    14461 cgccggcatc cgcgccaccg ccctggcccg cctgggcagc gacgatctcg aggggctgcg
    14521 catcgccgtg cagggcctcg ggcatgtcgg ctacgcgctg gccgggcacc tggccgccgc
    14581 cggtgccgag ctgctggtca gcgacatcga cgagagccgc gtgcagctgg cggtcgaacg
    14641 ctttggcgcg cagccggttt ccagcgaggc gctgctcacc gtgccgtgcg acatcctcgc
    14701 cccctgcggt ctgggtgggg cgatcaaccc gcagacggtc ggccagctgc gctgcgccgc
    14761 ggtggccggt gccgccaaca accagctgga cagcctggaa atgggcgacg agctggcggc
    14821 ccgcggcatc ctctatgcgc cggactacgt gatcaattcc ggcggcctga tcttcgtcgc
    14881 cctgcagcac caggggcgca gcccggcgga aatcaccgcc cacctggcgc agatcggcag
    14941 ccgtctcagt gaaatctatg ccgaggcgct ggccgagcag cgctcgccgg cgcgggtcgc
    15001 cgacagcctg gccgagcgcc tgatctacgg ttgctgacga tcagctcttg agccaccgcg
    15061 gcgcgaccgc caatgcacct cgcggtgcca gggaggatgc catgccccac agccgaattg
    15121 atctaccgta cacccgctac ctcgatgccg acggccgtcc gctggagccg ctgcccgact
    15181 gggtggagga cgaagcacgg gtggtcgggc tgtatcgcgc catgctgcgc acccgcctgt
    15241 tcgatcagaa ggtgatcgcc ctgcagcgca ccgggcgcat tggcacctat gcctccagcc
    15301 tggggcagga agccatcggc accgccatcg gcagcctgct gcgtgacgac gacgtgttcc
    15361 taccgtacta ccgcgacagc gccgcccagc tgctgcgcgg ggtgagcatg accgagatgc
    15421 tgctgtactg gggtggcgac gagcgcggca gcgcctatgc aggcccggtg gcgcgcggcg
    15481 acttcccgtt gtgcgtgccg atcgccaccc aggcgctgca tgcctgcggg gtggccagcg
    15541 cgttcaagat ccgcggcgag gcgcgggtgg cgctgaccac ctgcggcgac ggcgccacca
    15601 gcaagggcga cttcctcgag gccctgaacg tcgccggcgc ctggcagctg ccggtggtgt
    15661 tcgtggtcaa caacaaccag tgggcgatct cggtgccgcg gcgcatccag tgcggcgcgc
    15721 cgaccctggc gcagaaggcc atcggcgccg gcttcgccgg cgagcaggtg gacggcaacg
    15781 acgtgctcgc ggtggccgag cgcctgcgcc aggccatcga ccatgcccgc cacggcaagg
    15841 gaccgaccct gctggagtgc atcacctacc gcctctgcga ccacaccacc gccgacgacg
    15901 ccacccgcta ccgcagcgcc gaggaggtca accaggcctg gcacgaggaa ccgatcaagc
    15961 gcctgcagaa ctttctggtg gcgcgcggct ggtgggacga gcagcgcgag caggcgctgg
    16021 tggccgagac ccaggccgag ctgcagcagg ccatcgacgc ctacgaggcg accccggccc
    16081 aggatctgca ggaggtattc cgccacgtct acgccgactg gccggcggcg ctggccgagc
    16141 agcgcgagca gttcgccgag cgcgtcgccc gccgcacgca aggagcggat catgagtgag
    16201 aaaaagtaca ccctgctgga ggcggtcaac ctggccctgc accgggcgat ggccgaggac
    16261 gccaacgtgg tggtgctcgg cgaggacatc ggcgtcaacg gcggcgtgtt ccgcgctacc
    16321 ctcggtctgc gcgaggcgtt cggcttcaag cgggtgatcg acacgccgct ggcggagaac
    16381 atgatcgccg gcctgtccat cggcatggcc agccagggcc tcaagccggt gatggaaatc
    16441 cagttcctcg gcttcatcta cgcggcgatg gagcagctgg tgtcccacgc cagccgcctg
    16501 cgcaaccgca cccgcggccg gctgagctgc ccgctggtgc tgcgctcgcc gatgggggcc
    16561 ggcatccgcg cgcccgagca ccacagcgaa gccaccgagg cgctgttcgc ccatatcccc
    16621 ggcctgcgcg tggtcatccc ttcgtcgccg gcgcgggcct acggcctgct gctcgccgcc
    16681 atcgacgatc ccgacccggt gatcttcctc gagccgaccc gcctgtaccg gatgaacccg
    16741 cagccgctgg tcgacgacgg ccgccgcctg ccgctggaca gctgcttcac cctgcgcgag
    16801 ggcagcgata tcaccctgat cagctggggc gccagcgtgc acgagaccct gcaggccgcc
    16861 ggcgccctgg ccgagcgcgg ggtcagcgcc gaggtgatcg acgtggccag cgtcaagccg
    16921 ctggacctcg acaccctgga agcctcggtg cgcaagaccg ggcgctgcgt gatcgtccag
    16981 gaggcgccga agagcggcgg ggtgggtgcg gagatcgccg ccagcctgta cgagcgcgtg
    17041 ctgctcgacc tgcaggcgcc gatccagcgg gtcgccgcgc cggacatccc gccgccgctg
    17101 taccgcctgg agccgtacta cctgccggac gtcggcgaca tcctcgccgc ctgcgacgcg
    17161 gtgctggagt acgcctgagc tgcctgacgg acgtagggtg gaaaactcgc gcagcgattt
    17221 tccaccgcca tgcacaaagg tggacaaggc tgcgccgttg tccaccctac ggttgatgct
    17281 cccgtggccg cgacaggtca gggaggaact gggggaaccg aaccatgaaa cacttcaagc
    17341 tgcccgacct cggcgaaggc ctgcaggagg cggagatcgt cgagtggcac gtcaagcccg
    17401 gcgacagcgt caaggccgac cagttgctgg tctcggtgga aaccgccaag gccatcgtcg
    17461 acattcccgc cccctatgac ggcgtggtgc tgaagaccta cggcgtcgat ggcgacatca
    17521 tccacgtcgg cgagcccctg ctggcctacg agggcgaggg cgaggacagc ggcaccgtgg
    17581 tcggcaagct ggaggccggc gagggcggcg agggcgacag cttcttcgtc ggcgccgcgc
    17641 cgtcgacccg cgagcacctc gccccgcgcg ccactccggc ggtgcgccag ctggcgcgtc
    17701 agctcggcgt ggagctgggg gggctgaccg gcagcggcgc cgatggcctg atcacccgtg
    17761 ctgatgtcga ggcggtggcg gaaaa
//
LOCUS       DATCUN010000194        27436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_46024, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000194 DATCUN010000000
VERSION     DATCUN010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 27436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27436
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46024"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="VIO83_07790"
     CDS             <1..1512
                     /locus_tag="VIO83_07790"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8331213.1"
                     /translation="GVPALHWLGGERLREFARRLQELPQQPVATPAGLHAELRDYQQH
                     GLAWMQALRELKVGGILADDMGLGKTLQTLAHILAEKQAGRLTRPALIVMPTSLIPNW
                     QDEAARFAPQLRVLALHGAKRKALFEQIGEHDLVLTTYALLPRDLKQFVGRGWHLLIL
                     DEAQNIKNPRSRAAQAAGQLSADQRLCLTGTPLENHLGELWSLFNFLMPGWLGDSKAF
                     TRTYRTPIEKKADALRLAHLVARVRPFLLRRTKEQVARELPPKTEITQRIELTDVQRD
                     RYEALRLAMDQKVRAEIQRLGIGRSQIVILEALLRLRQACCDLRLLGDEGAEHGSADS
                     GKLSALLEMLEELTSEGRRVLLFSQFTSMLGLIEGELKARKIAYAKLTGATQDRRAPV
                     QAFQSGAVPVFLISLKAGGAGLNLTAADTVIHFDPWWNPAAEAQASDRAYRIGQDKPV
                     FVYKLIARGSVEEKIQQLQQSKASLARSVLEGGGQGDWALSEADLNALLAPLA"
     gene            1525..2169
                     /locus_tag="VIO83_07795"
     CDS             1525..2169
                     /locus_tag="VIO83_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEY8331214.1"
                     /translation="MRFSDTGNAPAQSIACEQRDYPEWHRGRARYGVWTLPVECPQVL
                     ARVARAQAHLGDWLHQGYRRQAHITLFVCGFPAEQALLDDDFPLARLAAQQQALRELG
                     AAPFELHISGLDSFASAPFLSVTDPAGRLEALRTVLAGHSAEIRQSPYHAHLTVGLYG
                     RTVLRADLQQRLAAFDEDEPLALGVDELHYSTYAATELFGPLHTERCLDLRRWS"
     gene            2380..4575
                     /locus_tag="VIO83_07800"
     CDS             2380..4575
                     /locus_tag="VIO83_07800"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase"
                     /protein_id="HEY8331215.1"
                     /translation="MAKKSGIISGIGNASVFVDLDGDGVRDSNEIGTVADASGKFILI
                     GAGKALKGPLVVEGGFNLATDLANTLTLTAASNAKVVNSLTTLIQSIAHATGTAPSVA
                     AKSLLLSLGLKGISLLTLDPAKTADLGKAGSPKVTNALKALQVQEQLDALLKMGGAVL
                     AGASGTNSGTLQTGLLDAIARQIADGKQLRLDDATQIAEILATAADSAGLSTEQRDLV
                     VSVAADAASVIVMTTGSITQLIGTVLAKGKSATAATVDGVLATLGHLEKSSNSIAGSL
                     KTAVSESLVAALLDEQLKSNTPEQQAISKSQTISGVQTSELLDTSSYIVQALDISLES
                     SFSKWGKTDLTYSYEQSQPADHSNQDDIVNWQPVPAFAQGQIDALMQHAEAISGLDIG
                     KVDSNGDIRLGVVDTLSTTYAYAYFPEVSGQLAIAGDLFLGSNLISDATESFADGTYA
                     YFGVLHELGHALGLKHPFEGVSILPESEDVHANTVMSYTESHALVPMLSWADGPVSFS
                     TESVYPHTFMVYDIAALQSLYGADMATATGNDTYAYGDDPFYETIWDAGGTDTLDFSL
                     TTHQNAIDLTPGSYSTVNYRSLDTQIAEAQATLTAALGHSYYDHFVEKTFNSLADEFF
                     TGEGALGIAYGVVIENAIGGGANDTFFDNEVDNVLYGNAGNDVFNLGTGGYDTVYGGD
                     GDDLLALSMSESSVWITSSASEALLIADAFAVKLVGVESIQFSDSVYYV"
     gene            complement(4622..6778)
                     /gene="speC"
                     /locus_tag="VIO83_07805"
                     /pseudo
     CDS             complement(4622..6778)
                     /gene="speC"
                     /locus_tag="VIO83_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798143.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            7088..9388
                     /locus_tag="VIO83_07810"
     CDS             7088..9388
                     /locus_tag="VIO83_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HEY8331216.1"
                     /translation="MQKRSSIAQENFTARVSKAFKEELARSVNPWELYNDWYRYGVDV
                     AERSLLFWDTLRQRGNDYLEHLQQGQPPLLHFDYETVLDGRSFERPVNYALLRILPPA
                     GVQVDPKRRPYLILDPRAGHGPGIGGFKDDSQVGVALRAGHPVYFVSFFREPEPGQTL
                     LDVCAAEQRFVRKVRELHPDSHKPALVGNCQGGWAAMMLTASVPDEAGPVVINGAPMS
                     YWGGAWQEGEGDNPMRYSGGLLGGTWLASLTADLGNGIFDGAWLVQNFENLNPANTFW
                     DKYYHLFAHIDSEPPRFLEFERWWGGYFLMNREEIEWITRNLFVGNRLWSGETRNASG
                     EAFDLRAIQSPIILFASLGDNITPPQQAFNWVADVYGSTEEIKARGQVIVGLLHEEIG
                     HLGIFVSGKVARKEHAQLVSVMQHIEMLPPGLYGMEIIERRSADGQIDYEVEFHEKRL
                     EEIVARLNRFQRADEKPFEAVAELSEFNQRAYQLFAQPWVQAMSTAASAELLRELHPL
                     RVQHWGISDRNPWLWWLAPAAAAVKAQRQKTDADALPWHWERFGAELISASLDHYRAL
                     RDATSEAQFFQVFGSLSGLYLADRAEAKTVAAPGTIEPRQLAFVEEALASMREGGYSE
                     AFARVSALLARRGELPLSLLATRHELAQDYADLLPAQSPETWRRIRGEQEIIAHYEPE
                     QAIASLPDLLAETADRERLLTLLERLLEDERLQQTSPTAEQLAMLARIRGVLSGKPVT
                     RQRATSRRAKGWSNSAKTEHHHDRQA"
     gene            9372..10325
                     /locus_tag="VIO83_07815"
     CDS             9372..10325
                     /locus_tag="VIO83_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEY8331217.1"
                     /translation="MTAKHEKYRRLIEFCRELPPTPTAVAWPCDQGSLQGVVDAAREG
                     LIAPILVGPRERIEALAAQHGINLDGLPIEDAPYSQAAAARAVELVREGKAEALMKGS
                     LHTDELMGAVVKRETGLRTARRVSHCFVMDVPGHADPLIITDAAVNIAPTLAEKMDIT
                     QNAIDLAHALLVGEQDEDVRVAILSATETVNPAVPSTVEAAALCKMADRGQITGAILD
                     GPLALDNAISLEAAQIKRIDSPVAGRANVLVVPDLEAGNMLAKSLTFLAGADAAGIVL
                     GARVPIILTSRADSLLSRLASCAVAVLVAKARRESAGKAIG"
     gene            10330..11514
                     /locus_tag="VIO83_07820"
     CDS             10330..11514
                     /locus_tag="VIO83_07820"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEY8331218.1"
                     /translation="MEQVILVLNAGSSSLKFSLFIAHQAEPELRLRGQIEGINGAPHF
                     IARDTAGAILAEQRWSEDSQLGHAGAALHLVEFLRGFRDRYQLIAVGHRVVHGGQEFT
                     GPTLVDGEILERLARFNPLAPLHQPHNLAPIRAIAERAPQLPQVACFDTAFHRRQPEL
                     AQMFALPPAITERGVRRYGFHGLSYEYIASTLPQLDPAAAAGRVVVAHLGNGASLCAM
                     LAGRSMASTMGFTAVDGLPMGTRCGNIDPGVILYLLDELKMDARAIERLIYKESGLLG
                     VSGISSDMRELLASDAPSAGLAVDLFVYRIGRELGSLAAALGGLDALVFTGGIGEHAA
                     PIRERVCRAAAWLGLQFDAAANADGGPRISAPSSPVSAWVIPTNEELMIARHTRQAIL
                     PI"
     gene            11534..12316
                     /gene="fabI"
                     /locus_tag="VIO83_07825"
     CDS             11534..12316
                     /gene="fabI"
                     /locus_tag="VIO83_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY8331219.1"
                     /translation="MTPEIPRPVLKGAKALVVGVANEHSIAYGCAKAFAELGAEVAIT
                     YANDKTRSYVEPLARELNAPIFMPLDVSQPGQLEAVFAEIERHWGQLDILVHSIAWAP
                     KEDLQGGLLDSSAEGFGLAMDISCHSFVRMARLAAPLMRNGGTMFAMSYYGANKVVPN
                     YNLMGPVKAALEASCRYLAYELGPQGIRVHAISPGPLKTRAASGLKDFDLLLNEAAQR
                     APLGELVDIMDVGFTCAWLATPYARRLSGETLYVDGGVNIMA"
     gene            12701..13465
                     /locus_tag="VIO83_07830"
     CDS             12701..13465
                     /locus_tag="VIO83_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEY8331220.1"
                     /translation="MPIKYARRLTDRRRSKQSNRQLGLCLAFIAGAANAGGFLAVRQY
                     TSHMTGILSSVADNLVLGKTDLALGGIGGFLSFVFGAISSTLLINFSRRRRLHSQFAL
                     PLALEAGLLLCFGVLGARLMEVPGFFVPLTVMLLCFIMGLQNAIITKLSNAEIRTTHI
                     TGMVTDIGIELGKALYVNRHDRANQIPVHANRERLRINILLVGCFFVGGVSGAFGFQH
                     FGFLATVPLAALLLTLAAVPMVDDLRLNWWLWRRRR"
     gene            complement(13557..13781)
                     /locus_tag="VIO83_07835"
     CDS             complement(13557..13781)
                     /locus_tag="VIO83_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010211805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HEY8331221.1"
                     /translation="MRKHPVMGATLMLCIGLLAGCSTPAAITLKDGRELQSLDTPEFD
                     EDSGFYEFKQPDGKLTRLNKDEVRSIKDLD"
     gene            14209..15279
                     /locus_tag="VIO83_07840"
     CDS             14209..15279
                     /locus_tag="VIO83_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY8331222.1"
                     /translation="MKQQQKWIGRGVLLAALIGGGWLVWQGLQPKGLGDGFVSGNGRI
                     EATEIDVATKLAGRIAEIGVDEGDFVQPGQVLARMDTEVLQAQLRQAEAQVRQAQNAI
                     LTARAMVAQRESEKATAEAVVLQRQAELTAAQKRHQRTETLVTRNAASRQQLDDDLAA
                     RLGAQAAVTAARAKVLSDQAAIEAAKSQVIEAESTVEAAQATAARLQADINDSALKTD
                     RVARVQYRIAQAGEVLGAGGKLLNLVDLADVYMTFFLPERQAGRVALGSEVRLVLDAA
                     PQFVIPAKVSYVASVAQFTPKTVETASEREKMMFRVKARIDPELLRKYQDQVKTGLPG
                     MAYLKLDPEAEWPTHLQLNAQQ"
     gene            15276..18020
                     /gene="rbbA"
                     /locus_tag="VIO83_07845"
     CDS             15276..18020
                     /gene="rbbA"
                     /locus_tag="VIO83_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HEY8331223.1"
                     /translation="MSEAPVARLDNVSLSYGKTQALDGVSLEVPARRMVGLIGPDGVG
                     KSSLLALVAGARQIQQGSVQVLDGDMASRHHRRVVCPRIAYMPQGLGKNLYPTLTVFE
                     NLEFFGRLFGQAREERRWRIDGLTRSTGLHPFLDRPAGQLSGGMKQKLGLCCALIHDP
                     DLLILDEPTTGVDPLSRNQFWELIEHIRADRPQMSVLVATAYMDEAQRFDHLVAMDAG
                     RVLATGTPAELLARTASASLEEAFIRLLPEEKRRQHRELVITPRQHNDSIAIEAHGLT
                     MRFGDFVAVDQVNFRIERGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAFLFGKPVD
                     PHDMQTRQRVGYMSQSFSLYSELTVLQNLDLHARLFHIPAERRAARIGEMLERFGLSA
                     EADSLPTSLPLGVRQRLSLAVAVIHNPEILILDEPTSGVDPIARDGFWELLVQLSRQD
                     GVTIFISTHFMNEALRCDRISLMHAGRVLDSDTPQALMDKRGLPSLEATFIAYLEEAA
                     AENAASLPAPASSPAPVAAAPAAPGRTADRQSRFSLRRLLSYSRRESMELRRDPIRAT
                     MAMLGTVILMFIMGYGISFDVENLSYAVLDRDQTSTSQSYLLNMSGSRYFIEQAPLAS
                     HAELDQRLRSGDISLAVEIPPNFARDLKRGSPPQVAFWIDGAMPMRADTIKGYVQGIH
                     LSYLQQLAREAGVDNLGAPTEVAVRYRYNPDVQSLPAMMPAMIPLLLMMIPAMLTALG
                     VVREKELGSITNFYVTPTTRLEFLIGKQLPYIALGMVNFAMMVLLAVYLFGIPVKGSL
                     LTLTFGALLYIACATGLGLLMSSILNSQIAAIFGTTIATLLPAIQFSGLTHPVSALEG
                     ASAFIGQLYPTSHFLIISRGVFSKALNLADLYPYFIPMLLTIPVLTLLSIAGLRKQEK
                     "
     gene            18022..19149
                     /locus_tag="VIO83_07850"
     CDS             18022..19149
                     /locus_tag="VIO83_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8331224.1"
                     /translation="MSASRRKLANIFQLGLKELRSLYRDPALLILIIWSFTMAVYSSA
                     TAIPEAPHRASIAVVDEDQSQASRRIINAFQLPYFIQPAAIDLAQMDSGMDAGLYTFT
                     LNIPPGFQEDLLAGRQPTIQLNVDATQVSQAFTGAGHIQQIIATETAEFAQRYRSSEA
                     LPIEAVVRTAFNPNLTRAWFGAVNEVINQITILAIILTGAALIREREHGTIEHLLVMP
                     VTPFEIMLGKVWSMGLVVLAASAFALRFVVEGWLDIPLQGSTLLFTGGAALHLFAVTS
                     MGIFFATVARSMPQLGLLTILTLMPLQILSGGTTPRESMPELVQNVMLAAPTTHFVEL
                     AQAILFRGADLNIVWPQLLALATIGSMFFIGALSRLRKSLR"
     gene            19201..20652
                     /locus_tag="VIO83_07855"
     CDS             19201..20652
                     /locus_tag="VIO83_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY8331225.1"
                     /translation="MNRTPGTLRKLALAIALSSAPTLLLAEPAAPSPRADLIGVYQQA
                     LENNADLAAARADYLARKEVVPQARSGLLPQLNGGASLGDTKTELDQPSVSLERSGTL
                     YQASLSQALFRADRWFQLQAAKAVSEQAALQFSSVEQNLVLQSAEAYFAVLRAQDGLA
                     ASRAEEAALKRHYDQANDRFEVGLSDRTEVLEAQSSYDTARANRILAERLVEDAFQAL
                     ATLTNRDYSFIEGIRHTLPVSPPAPNDAKAWVDTAMQRNLDLQASQYAVTGAEETLRQ
                     RKAGHAPTVDLVAQYQKGDNDRLGFTNTALAGRIGSTISGDVEQQSIGVELNIPLYSG
                     GLTNSQVRESYQRLNQTEQLRESLRRQVVQNTRDFHRAVNTDVAQVAARRQAIVSSQS
                     ALEATELGYEVGTRNIVDVLDAQRQLYLAVRLYNNTRYDYILNHLRLKRSAGILSPAD
                     LQDLESFLKPDYNPDQDFLPPGLAPSLSGRQGE"
     gene            20832..21359
                     /locus_tag="VIO83_07860"
     CDS             20832..21359
                     /locus_tag="VIO83_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3087 domain-containing protein"
                     /protein_id="HEY8331226.1"
                     /translation="MFEIKQLDPETYRRQTRRSTLIIVMIFALLAMLLSSMAVLLFGE
                     AGGDNFRWNMGGVIAGLLLTGALVRLHFWSQPWMAPAVYGWQLKRSLMSVTNVMHHVT
                     AGVQARDPAAMKLLRFYHLGLTQMHQLDANSSALSQMVREINQHRECMEALHMDTDQY
                     RLDPAWLEAVKKAGA"
     gene            complement(21403..22172)
                     /locus_tag="VIO83_07865"
                     /pseudo
     CDS             complement(21403..22172)
                     /locus_tag="VIO83_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019690659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
     gene            complement(22257..23429)
                     /locus_tag="VIO83_07870"
     CDS             complement(22257..23429)
                     /locus_tag="VIO83_07870"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY8331227.1"
                     /translation="MTRRDAKTVTLVLGAALALTAYGGDRGGAKYPTLNGDKPLVVGH
                     RGAAGYLPDHTLEGYRKAIELGADFIEPDLVATRDGVLVARHEPNITSTTDVRGRPEF
                     ASRKTRRTVDGVEEEGWFVTDFTLAELKTLRAIQPFADRDQSYNGKLQIPTFDEVLDL
                     ARSEGARLGRSIGVYAETKHPTYHARLGLKLEDRLLDTLARYGYTSKASPVILQSFEV
                     SNLQYLRGKTQLRLVQLVDASDINADGSIQLAAPFDKPYDFAVAGDARTFASLLTPAG
                     LKEVKTYADGIGPWKPYLIPSRLLDDNHDGQPDDLNGDGQLDERDRVMMPPTEVVRNA
                     HAAGLFVHAYTFRSEAKRLASDFKGDPGAEYRLFYTLGVDGVFSEFADHARAARDD"
     gene            complement(23705..24472)
                     /locus_tag="VIO83_07875"
     CDS             complement(23705..24472)
                     /locus_tag="VIO83_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8331228.1"
                     /translation="MAIPYLDLAPSGLFATFARNDRGRDLLVGDIHGQASLLEHLLTK
                     AGFDRRVDRVFAAGDLVDRGPQSLQVLALLDEPWFHSVRGNHDQMCVDGASGFSTEQH
                     MMHGGGWFYQLGSRHRQDVSERLRQLPIAFEIEGRNQLRFGLVHGECPLSDWQRFREV
                     LSAPLERTRGNTDGLLERMTVIGDAMWNKARLEQQDTSLVTGIDQIYVGHSRVRAILQ
                     LGNTTFIDTGNCFENGSLSLIDLHTQTTWSARLHELT"
     gene            24653..27301
                     /locus_tag="VIO83_07880"
     CDS             24653..27301
                     /locus_tag="VIO83_07880"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY8331229.1"
                     /translation="MAFPLVNPSQLRSPSLTDKTLARPRLLQRLQESASRGCRLTLLS
                     APLGYGKSTLLAQYAASLQTPWAWYRLDNADNQPLHLLLHLHAALGLATPERDLVAAD
                     EPRLWSAILQRLEHAADHFTLILDDLHLLRAGRARHYLEQLLRFGPPGLHLLAACEGQ
                     PELALSHLRREEQLTVLDARELALDSEETRQLANSRGAKLGTDALYYELRANSEGWIS
                     GLLLWLTAYREQPPASQSRRDLRNVTRQAYAYVTRFLEEELLAQLPSELLAFLESTAV
                     VQTFDPTLAGILSGQPNAERLIRQLLRRDLFIEQRPGERVEYRYHAVLRGVLYQRLQQ
                     RDPQLLQQLHQQAAAWLLEQRRYSEAIHQYGRARDFNAVLAIAERHTFDLLRDGQINA
                     LVDLLSEVSGQNSSDHFTLAISEASIVLATNDIASAQACIRRLQRQNVPRHPERVQQT
                     IAYLRTLLAFLGGNLRHGLELASRALQRYPQRNTAGSVLRFNRARCLFHLGLLGEARL
                     DVTQTLEELNDFGFSGFANIPHLLLGQIELAQGDSDAAMKRFLALERESPATASRNFY
                     ELFHHLGLGLVFVQHNRLEQARQCLAKAEALALSFPHTAGLVRVLHYQACLFDALGDS
                     NMAQARWDEVRRMARQFRQFGLYRLAGAWRVRLAVRERDEDFILQWLQEWHWCQRHYG
                     EDLLPEEWLAYAWVQRHLGQPAVAGQILDNLREQAESEGNRRLQLDLWLLDATLRRDH
                     GDQMAALACLDDALHLAVSYSLGQMLHHEGRELAELFRQLLLPQVRRHAGLERPLPSR
                     DLLAAALTGLVSDSTMHMLLEPITRRELDVLRRMARGQNNQQLADGLFVSLSTVKTHI
                     NNLFRKLDVTDRDAALRAARELQLLD"
     gene            complement(27318..>27436)
                     /locus_tag="VIO83_07885"
     CDS             complement(27318..>27436)
                     /locus_tag="VIO83_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY8331230.1"
                     /translation="AAGKLPKVALVACMRKLLTTLNAMARSGLPWNPEFKTA"
BASE COUNT         4794 a         9511 c         8646 g         4485 t
ORIGIN      
        1 ggggtacccg ccttgcactg gctgggcggc gagcgcctgc gcgaattcgc ccggcgcctg
       61 caggaattgc cgcaacagcc ggtggccacg ccggccggcc tgcacgccga gctgcgcgac
      121 tatcagcagc acggcctggc ctggatgcag gcgctgcgcg aactcaaggt cggcggcatc
      181 ctcgccgacg acatgggcct gggcaagacg ctgcagacct tggcgcacat cctcgctgag
      241 aagcaggccg ggcgcctgac ccgcccggcg ctgatcgtca tgcccaccag cctgatcccc
      301 aactggcagg acgaggcggc gcgcttcgcc ccgcagctgc gcgtactggc cctgcacggt
      361 gccaagcgca aggcgctgtt cgaacagatc ggcgagcacg atctggtgct gaccacctac
      421 gccctgctgc cgcgcgacct caagcagttc gtcggccgcg gctggcatct gctgatcctc
      481 gacgaggcgc agaacatcaa gaatccgcgc agccgcgcgg cccaggccgc cggccagctc
      541 agcgccgacc agcgcctgtg cctgaccggc acgccgctgg aaaaccacct cggcgaactg
      601 tggtcgctgt tcaacttcct gatgcccggc tggctcggcg acagcaaggc cttcacccgc
      661 acctaccgca cgccgatcga aaagaaggct gacgccctgc gcctggccca tctggtcgcg
      721 cgggtcagac ccttcctgct gcgccgcacc aaggagcagg tggcgcgcga actgccgccg
      781 aagaccgaga tcacccagcg catcgagctg accgacgtgc agcgtgatcg ctacgaggcc
      841 ctgcgcctgg ccatggacca gaaggtccgc gccgagatcc agcgcctggg catcggccgc
      901 agccagatcg tcatcctcga ggccctgctg cgcctgcgcc aggcctgctg cgacctgcgc
      961 ctgctcggtg acgaaggcgc cgagcacggc agcgccgact ccggcaagct gagcgccctg
     1021 ctggagatgc tcgaggagct gaccagcgag ggccgccgcg tactgctgtt ctcccagttc
     1081 accagcatgc tcggcctgat cgaaggcgag ctgaaggccc gcaagatcgc ctatgccaag
     1141 ctcaccggcg ccacccagga ccgccgtgcg ccggtgcagg cgttccagag cggtgcggtg
     1201 ccggtgttcc tgatcagcct caaggcaggc ggcgccggcc tcaacctgac cgccgccgac
     1261 accgtgattc acttcgaccc ctggtggaac ccggccgccg aagcccaggc cagcgaccgc
     1321 gcctaccgca tcggccagga caagccggtg ttcgtctaca agctgatcgc ccgcggcagc
     1381 gtcgaggaga agatccagca gctgcagcag agcaaggcca gcctcgcgcg cagcgtgctg
     1441 gagggcggcg gccagggcga ctgggccctc agcgaggccg acctgaacgc cctgctggcg
     1501 ccactggcct aggtgcgggc ctgagtgcgg ttctccgaca ccggcaatgc ccccgcgcag
     1561 agcatcgcct gcgagcagcg cgattacccg gaatggcacc gcggccgcgc ccgctacggc
     1621 gtgtggacac tgccggtgga gtgtccgcag gtcctggctc gagtggcgcg ggcgcaggcc
     1681 catctgggcg actggctgca tcaaggctac cggcgccagg cgcatatcac cctgttcgtc
     1741 tgcggcttcc ccgccgagca ggcgctgctg gacgatgact tcccccttgc gcgactcgcg
     1801 gcccagcaac aggcgttgcg cgagctgggt gccgccccgt tcgagctgca catcagcggc
     1861 ctggacagct tcgccagcgc gcccttcctc agcgtgaccg atcccgcagg ccggctggaa
     1921 gcgctgcgga cggtactggc tgggcacagc gcagagatcc gccagagccc ctaccacgcc
     1981 catctcaccg tgggactcta cggccgcacc gtactgcgcg ccgacctgca gcagcgcctg
     2041 gcggccttcg acgaagatga gccgctagca ctaggcgtgg acgagctgca ttacagcacc
     2101 tatgccgcca ccgagctgtt cggcccgctg cacaccgagc gctgcctcga cctccgccgg
     2161 tggagctagc cccagacggt ctggctgcta cgccggctac tggctgaacg actcgccatt
     2221 gagagctgct tcgccatgct cctcgccggg acggtatcat ccgccccgaa cttgccacgc
     2281 agcagaactc atgctggccc gcagaccact cgaaatcggc tggcgatctc ccattgcggg
     2341 tcgcccagcc tcggcaagaa atcaggatag gaaatcctca tggcaaagaa gtccggcatc
     2401 atcagcggta tcggcaacgc cagcgtattc gtggatctgg acggcgatgg cgttcgggac
     2461 agcaacgaaa ttggcactgt cgccgatgcc agcggcaagt tcatcctgat cggcgccggc
     2521 aaggcgctga aaggccctct ggtggtcgag ggcggcttca acctcgcaac cgacttggcc
     2581 aacaccctga cgctcactgc tgccagcaac gccaaggtgg tgaactcgct gaccaccctg
     2641 atccagtcga tcgcccacgc aaccggtacc gccccgagcg tcgcagccaa gagtctgctg
     2701 ctatcgctgg gactcaaggg aatcagcctg ctgacgctcg acccagccaa aactgccgac
     2761 ctcggcaagg ccggcagtcc aaaggtgacc aacgccctca aggccctgca ggtacaggag
     2821 caattggatg ctctgctgaa aatgggcggc gccgtgctgg ccggagcatc cggcacaaat
     2881 agcggcaccc tgcagacagg tctgctcgat gccattgccc gacagattgc cgatggcaaa
     2941 caactgcgtc tcgacgatgc gacgcagatc gccgagatcc tcgcgacggc cgccgattcc
     3001 gccggtctca gtaccgaaca gcgcgatctt gtcgtcagcg tggccgctga cgcagccagc
     3061 gtgatcgtca tgaccaccgg cagcatcacc cagctaatcg gcaccgtact ggcgaaggga
     3121 aagagcgcga cggcggcgac ggtcgatggc gtgctcgcaa ccttggggca tctcgaaaaa
     3181 tccagcaaca gcattgccgg ctcgctgaaa accgccgtta gcgaaagcct ggttgctgct
     3241 ctgcttgatg aacaattgaa gagcaatacg cctgaacaac aggccatatc gaaaagccag
     3301 accatttctg gcgtacaaac cagcgagttg ctggatacca gcagctacat cgtccaggcg
     3361 ctggacataa gcctggagtc gagcttctcg aagtggggca agaccgacct cacctacagc
     3421 tacgagcaat cgcaaccggc cgatcacagc aaccaggacg acatcgtcaa ctggcagccc
     3481 gttccggcgt tcgcacaggg ccagatcgac gccttgatgc aacatgccga agccattagc
     3541 ggtctcgaca tcggcaaggt ggacagcaac ggcgacatcc ggctcggcgt agtcgataca
     3601 ctcagcacca cctatgcata cgcctacttt ccagaggttt ccgggcagct tgccatcgct
     3661 ggggatctgt tcctcggcag caacctgatc agcgatgcga ccgaaagctt cgctgacggt
     3721 acctacgcct attttggcgt cctccatgaa ctagggcatg cactgggcct gaaacaccct
     3781 ttcgaagggg tgtcaatcct gcctgaaagc gaagacgttc atgccaacac cgtgatgtcg
     3841 tacaccgaaa gccacgcgct cgtgcccatg ctttcctggg cagacggccc ggtatccttc
     3901 agcacggaga gcgtctaccc gcacaccttc atggtgtacg acattgctgc cctgcaaagc
     3961 ctttatggcg ccgacatggc cacggcaacc ggcaacgaca cctacgccta cggcgatgat
     4021 cccttttacg aaaccatctg ggatgccggc ggcacggata cgctggactt ttccctcacc
     4081 acgcaccaga acgccatcga cctcacgccc ggcagctata gcacggtcaa ctaccgcagt
     4141 ctcgacaccc agatcgccga ggcccaggca accctcaccg ccgcgctcgg ccactcgtat
     4201 tacgaccact ttgtcgaaaa aaccttcaat agcctggcgg acgagttctt caccggcgaa
     4261 ggtgcactgg gcatcgccta cggagtcgtc atcgagaacg ccataggcgg cggcgccaac
     4321 gacactttct tcgacaacga agtcgacaac gtcctctacg gcaatgccgg caacgacgta
     4381 ttcaacctgg gcaccggagg ctacgacacc gtctacgggg gggatggcga tgacctgctg
     4441 gctctctcca tgagcgaatc cagcgtatgg atcacctcct ccgccagcga agccctactg
     4501 attgccgacg ctttcgccgt gaagctggtc ggggtggaga gcatccagtt ctccgattcg
     4561 gtttattacg tctgaacgtc agtcacagcg gtgccgaccc gacaccgccg tgactgctgc
     4621 gttactcctt gaccacgtag ccgtaggcac gcttgcggcc gtcggcttcc cgctgcaggt
     4681 agacgccctg gatttccggc tcgaagccgg gcagcgcgtt gacgccctcc tccatcgcca
     4741 ggaagtactt gagcgccgca ccaccccata cctcgccggg caccacgcag agcaccccgg
     4801 gcggataggg cagcgcccct tccgcagcaa tccgtccgtc gatctcggcc agcggcacca
     4861 gctcgacgtt gttgcgcagc aggtgctggt tggcttccca cggcgtcagg gcctggcggg
     4921 ggaagtgttc cttcctgaac atctgcttct gcagcttgtt catctcgtgg cgcttgtaga
     4981 agtcgtgcat ttccttgcac agccggcgca gcgtatagcc gctgtatttt tcgtgcgcgg
     5041 catggatggc cggcagcact tcgctcagcg gactgtcgtg gtcgatatgg cgctcgaact
     5101 gggcaatatg ggccaccagg tgctgcagct tggcgaagtt gtccgagggt gtgagcagga
     5161 acaggatgga gttgaggtcc gccttctccg gcactatgcc gttggcgcgc aggtagtggg
     5221 cgagcaccgc ggccggaatg ccgagcgagg catagccccc ggtggtcggg tcgatgccaa
     5281 aggtggtcaa tagcatcttg cacgggtcga cgtagtactg gtccctggcg tagccggcga
     5341 agtcgtgcca cttgtcttcc ggcttgaatt tgaagaagct caggtcgtcg atgatcatct
     5401 cggtttcgtg ctcggcccac ggcaggccgt ccaccagcgt cgggatgaat ggcttgatgt
     5461 acttgcaggt acgcagcagc agcttgcgcg cctccacgcc gttcttgatg cactcgcgcc
     5521 acaggttgcg ccccgccagc ccgtcatgca tctgcgcgtt gacatccagc gccgcgaaga
     5581 tcggatagaa cggactggtc gaggcatgca tcatgtaggc gttgttcagc cgcttgtggt
     5641 tgcagtagcg gggctggccc ttgatgtggc ggtccttctt gtggatctgc gacgactgcg
     5701 agaagcccgc ctgctgcttg tgcaccgact gggtgacgaa cagccccgga tcgtcagggc
     5761 ccaggtcgag cagcaacggc gagctgtcgt gcatcatcgg gatgaactgt tcgtagccga
     5821 cccaggccga gtcgaacagg atgtagtcgc acagtttgcc gatccggtcc accacctgtc
     5881 gggcgtcgta gatggtgccg tcgtaggtgc cgagctggat caccgccagg cggaacggcc
     5941 gctcctgctg cgccgtggcc ggatcgacct cggcaatcgc cgcgcggatg tactgttcgt
     6001 cgaagcagtg cgcgtcgatg ccgccgatga agccgtagca gttgcgcgcc gtctccagat
     6061 acaccggccg ggcgccgcag aggatcagcg cgccgaggtg gatggacttg tggttgttgc
     6121 ggtcgaacag taccaggtcg tccttggcca gcagcgcgct ggtcaccacc ttgttcgagg
     6181 tcgaggtgcc gttgagcacg aagtaggtct tgtcggcgtt gtagatgcgc gcggcgttca
     6241 tctacgcgtc gcgggccggc ccctcgtgga tcagcaggtc gccgagcttg acgtcggcat
     6301 tgcacaggtc ggcgcggaac acgttctcgc cgaagaattc gaagaacgcc cgtcccagcg
     6361 gatggccgcg gaagaactgc ccgccctggt gccccgggca gtcgaaggcc acgttgccgg
     6421 tcttgacgta ctccttcagc gccttgaaga acggcgggta gagcttctgt gtgtaggtgt
     6481 cggccgcctg ggcgatgcgc ttgccgttga agtggcgctc gcccttgccc accttcagcg
     6541 tgccggcaat gcgggaaagg atgtcttccg gcaccgtctc gtccggccgc agggcgacga
     6601 acagcgggat cgggaacccg gtatggccga tccgggtcag cacctcccgc gcctcgtccg
     6661 ccagcgccac cacagccgcc acattggtga agtcggtgct ggccaggtcg atcatcgggt
     6721 agtcgaggat gaaactgtcg aacagcgacg gcggacaggc gaccttgaac gtgctcatgc
     6781 ggatctccgg gcggaaagcc agttgcggca atcacctgaa tctagcctcg gccgtgagct
     6841 ttgccagcgc agtgcggcag ggcgccggac gcgcccgctg cgcagcgctt ggcctctttg
     6901 ccgtgcttgc cctggcgctg caaacgtcta tcccgctcgt tgccgaccag cggcatggcc
     6961 tacacgggga cagcaactag gcttctgtgc caagtcaaca gcacagggca gatacatgga
     7021 caaggattag gacatcgccg ccaggctggc acgcagccag gatatcgcag ccaagaccgg
     7081 caaggtcttg cagaaacgca gcagcatcgc tcaggagaat ttcacggcac gggtgagcaa
     7141 ggccttcaag gaggaactgg ccaggtcggt caatccctgg gagctgtaca acgactggta
     7201 tcgctacggc gtcgacgttg ccgagcgctc gctgctgttc tgggacaccc tgcgccagcg
     7261 cggcaacgac tatctggagc acctgcagca gggccagccg ccgctgctgc atttcgacta
     7321 cgagaccgtg ctggacgggc gcagcttcga gcgcccagtc aactacgcac tgttgcgcat
     7381 cctgccgccg gccggggtgc aggtggaccc gaagcggcgc ccctacctga tcctcgatcc
     7441 gcgcgccggg catggccctg gcatcggcgg cttcaaggac gattcccagg tcggcgtggc
     7501 cctgcgtgcg gggcatccgg tgtatttcgt gagcttcttc cgcgagcccg agccgggcca
     7561 gaccctgctc gacgtgtgcg ccgccgagca gcgcttcgtg cgcaaggtgc gcgagctgca
     7621 cccggacagc cacaagccgg cgctggtggg caactgccag ggcggctggg cggcgatgat
     7681 gctcaccgcc tcggtgccgg acgaagccgg cccggtggtg atcaacggcg cgcccatgtc
     7741 ctactggggc ggcgcctggc aggaaggcga aggcgacaac cccatgcgct attccggcgg
     7801 cctgctcggc ggcacctggc tggcctcgct caccgccgac ctaggcaacg gcatcttcga
     7861 cggcgcctgg ctggtgcaga acttcgagaa cctcaacccg gccaacacct tctgggacaa
     7921 gtactaccac ctgttcgccc acatcgacag cgagccgccg cgcttcctcg agttcgagcg
     7981 ctggtggggc ggctacttcc tgatgaaccg cgaagagatc gagtggatca cccgcaacct
     8041 gttcgtcggc aaccgcctat ggtccggcga gacccgcaac gccagcggcg aagcgttcga
     8101 cctgcgggcg atccagtcgc cgatcatcct gttcgcctcc ctgggcgaca acatcacccc
     8161 gccgcagcag gccttcaact gggtggcgga cgtctacggc agcaccgagg agatcaaggc
     8221 gcgcggccag gtcatcgtcg gcctgctgca cgaggagatc ggccatctgg gcatcttcgt
     8281 gtccggcaag gtggcccgca aggaacacgc ccagctcgtc tcggtgatgc agcacatcga
     8341 aatgctgccg cccggtctgt acggcatgga gatcatcgag cggcgcagtg ccgacgggca
     8401 gatcgactac gaggtggagt tccacgagaa gcgcctggag gagatcgtcg cccgcctcaa
     8461 tcgcttccag cgcgccgacg aaaaaccctt cgaggcagtg gccgagctgt ccgagttcaa
     8521 ccagcgcgcc taccagctgt tcgcccagcc ctgggtgcag gccatgagca ccgcggccag
     8581 cgccgaactg ttgcgcgagc tgcaccccct gcgcgtccag cactggggca tttccgaccg
     8641 caatccctgg ctgtggtggc tggccccggc tgccgcggcg gtcaaggcgc aacgccagaa
     8701 gacggatgcc gacgccctgc cctggcactg ggaaaggttc ggcgccgaac tgatcagcgc
     8761 ctccctcgac cactaccggg cgctgcgcga tgccacgagc gaggcgcagt tcttccaggt
     8821 cttcggcagc ctgtccggcc tctacctggc cgaccgggcc gaggcgaaga ccgttgccgc
     8881 acccggcacg atcgagccgc ggcagctcgc ctttgtcgag gaagccctgg ccagcatgcg
     8941 cgaaggcggc tacagcgaag cctttgcccg ggtgtcggcc ctgctcgcgc ggcgcggcga
     9001 gctgcccttg tcgctgctgg ccacccgcca tgaactcgcc caggactatg ccgacctgct
     9061 gcccgcgcaa tccccggaga cttggcgacg tatccgtggc gagcaggaaa tcattgccca
     9121 ctatgaaccc gagcaggcca tcgccagcct gccggatctg ctggccgaaa cagcagaccg
     9181 cgagcgcttg ctgaccctgc tggagcgtct gctggaagac gagcgcctgc agcagaccag
     9241 tccgaccgct gaacagctcg ccatgctggc gcgcatccgc ggcgtgctgt ccggcaagcc
     9301 cgtcacccgc cagcgcgcca ccagcaggcg cgcgaaaggc tggagcaact cggcgaaaac
     9361 ggagcatcac catgaccgcc aagcatgaga aatatcgacg gctgatcgag ttctgccgcg
     9421 agttgccgcc caccccgacg gcggtcgcct ggccctgcga ccagggttcc ctgcagggtg
     9481 tggtggatgc ggcgcgcgag ggcctgatcg cgccaatcct ggtcggcccg cgcgagcgga
     9541 tcgaagccct ggccgcacag catggcatca acctcgacgg actgcccatc gaggacgcgc
     9601 cctacagcca ggccgccgcc gcccgggcgg tggaactggt gcgcgaaggc aaggccgagg
     9661 cactgatgaa gggcagcctg cacaccgacg aactgatggg cgcggtggtc aagcgggaaa
     9721 ccggactgcg cacggcgcgg cgggtcagcc actgcttcgt gatggacgtg ccggggcatg
     9781 cagaccctct gatcatcacc gatgcggcgg taaacatcgc gccgaccctg gccgagaaga
     9841 tggacatcac ccagaacgcc atcgacctgg cccacgccct gctggtcggc gaacaggacg
     9901 aggacgtgcg ggtggcgatc ctttcggcaa cggaaaccgt caaccccgcc gtgccctcca
     9961 cggtcgaggc ggccgcgctg tgcaagatgg ccgaccgcgg gcagatcacc ggcgccatcc
    10021 tcgacggtcc cctggcgctg gacaacgcca tcagcctgga agcggcgcag atcaagagga
    10081 tcgactcgcc ggtggccggg cgcgccaacg tgctggtggt gcccgacctg gaagccggca
    10141 acatgctcgc caagagcctg acgttcctcg ccggggcgga tgccgccggc atcgtgctgg
    10201 gcgcgcgggt gccgatcatc ctcaccagcc gcgccgactc gctgctcagc cgcctggcct
    10261 cctgcgcggt ggcggtcctg gtggccaaag cccggcgcga gagcgccggc aaggcgatcg
    10321 ggtgagtcca tggagcaggt catcctcgtc ctcaacgccg gctcgtccag cctcaagttc
    10381 tccctgttca ttgcgcatca agcggagccg gaactgcgcc tgcgcgggca aatcgagggc
    10441 atcaacggcg cgccgcactt catcgcccgc gatacggcgg gcgccatcct cgccgagcag
    10501 cgctggagcg aagacagcca gctcggccac gccggtgcgg ccctgcacct ggtggaattc
    10561 ttgcgcggct tccgcgaccg ctaccagctg atcgctgtgg gccaccgcgt ggtgcacggc
    10621 ggccaggaat tcaccgggcc gacccttgtc gatggcgaga tcctcgagcg gctcgcccgc
    10681 ttcaatccac tggccccgct gcaccagccg cacaatctgg caccgatccg cgccatcgcc
    10741 gagcgggcgc cgcagctgcc tcaggtggcc tgcttcgata ccgccttcca tcgccggcag
    10801 ccggagctcg cccaaatgtt cgccctgccg ccagccatta ccgagcgcgg cgtgcggcgc
    10861 tacggcttcc acgggctgtc ctacgagtac atcgccagca ccctgccgca actggacccc
    10921 gcggcggcgg ccggccgggt ggttgtcgct cacctcggca acggcgccag cctgtgcgcc
    10981 atgctcgccg ggcgcagcat ggccagcacc atgggcttca ccgcggtgga cggcctgccg
    11041 atgggcaccc gctgcggcaa tatcgaccct ggggtgatcc tctatctgct ggacgagctg
    11101 aagatggacg cccgcgccat cgagcggctg atctacaagg agtccgggct gctcggggta
    11161 tccggcatct ccagcgacat gcgcgaactg ctggccagtg acgcgccaag cgccggcctg
    11221 gcggtggacc tgttcgtcta ccgcatcggc cgcgaactcg gctcgctggc cgcagccctc
    11281 ggcggcctgg acgcgctggt gttcaccggc ggcatcggcg agcatgcagc gccgatccgc
    11341 gagcgcgtct gccgcgctgc cgcctggctg ggactgcagt tcgacgccgc cgccaatgcg
    11401 gacggcggcc cgcggatcag cgcgccttcc agcccggtgt cggcctgggt gattcccacc
    11461 aacgaagaac tgatgatcgc ccgccatacc cggcaggcga ttttgccaat ctgaaaaccc
    11521 gaggaaaaag gacatgactc cggaaatccc ccgcccggta ctcaaaggcg ccaaggcact
    11581 ggtggtcggc gtggccaacg aacactcgat cgcctacggc tgtgccaagg ccttcgccga
    11641 actcggcgcc gaagtggcca tcacctacgc caacgacaag acccgcagct acgtcgaacc
    11701 gctggccagg gaactgaacg cgcccatctt catgcccctg gatgtcagcc agcccggtca
    11761 gctcgaagcg gtattcgccg agatcgaacg gcactggggg caactggaca tcctcgtcca
    11821 ctccatcgcc tgggcgccca aggaagacct gcagggtggg ctgctcgact cctcggcaga
    11881 gggcttcggc ctggccatgg acatctcctg ccactccttc gtgcgcatgg cccgcctggc
    11941 ggcgccgctg atgcgcaatg gcggcacgat gttcgccatg agctattacg gggccaacaa
    12001 ggtggtgccc aactacaacc tgatgggccc ggtgaaggcc gcgctggaag cctcctgccg
    12061 ctacctcgcc tacgagctcg gcccccaggg catccgcgtg cacgccatct cccccggccc
    12121 gctgaagacc cgcgccgcct ccggcctcaa ggatttcgac ctgctgctca acgaagccgc
    12181 ccagcgcgca ccgctgggcg agctggtgga catcatggac gtcggcttca cctgcgcctg
    12241 gctggccacg ccctatgccc ggcggctgtc cggcgagacg ctgtacgtgg atggcggggt
    12301 gaacatcatg gcctgaggac cggccccgga tgtccgggcg gccaggcatg cgggtcgatc
    12361 cggcgcctgg ttgcctatgc catgagctgt ccggctggcc gatgctcaga gacattcgac
    12421 atgggcctgg tcaccggccg ggcgctggcc ccacgcacta cgaccacgcg cactgcccct
    12481 tctgatcact tggtggggca tggaggggaa tgcctatcca tccagtcttc caattcatta
    12541 tgttcaatag cgtaccataa atatcatcct cgctccgtac tccgccggtc ggagcagccg
    12601 gcgtatcact cgatgccggc caatcgcccc cactggccgc acccggccgt ataatccctc
    12661 cacctaccct gcagcccgga cggatggaat caccggaacc atgcccatca agtacgcgcg
    12721 tcgcctcact gaccgtcgcc gctcgaagca gagcaatcgc cagctcggcc tgtgcctggc
    12781 gttcatcgcc ggtgccgcca atgccggcgg ctttctggcc gtcaggcagt acacctcgca
    12841 catgaccggc atcctttcct cggtggccga caacctggtg ctgggcaaga ccgatctggc
    12901 actgggcggg attggcggct ttctgtcgtt cgtgttcggc gccatcagct ccaccctgct
    12961 gatcaacttc tcgcgccgac gacgcctgca cagccagttc gccctgccgc tggcactcga
    13021 agccggattg ctgctgtgtt tcggcgtgct gggtgcacga ttgatggagg ttcccggctt
    13081 cttcgtgccg ctcaccgtga tgctgctgtg cttcatcatg ggcctgcaga acgccatcat
    13141 caccaagctc tccaacgccg agatccgcac cacccacatc accggaatgg tcaccgacat
    13201 cggcatcgag ctgggcaagg cgctctacgt caatcgccac gaccgcgcca accagatccc
    13261 cgtgcatgcc aaccgcgaac gcctgcgtat caacatcctg ctggtcggct gcttcttcgt
    13321 cggtggggta agcggcgcgt tcggcttcca gcacttcggc tttctcgcca ccgtaccgct
    13381 ggccgcgctg ttgctgaccc tggcggcagt gccgatggtg gacgatctgc gcctgaactg
    13441 gtggttgtgg cgccgacggc gatgacgctg cgcgaggcgc tacccacgac agcacgcgca
    13501 agcgctgcgg aaatagcgca accagcgagt gcaggcatca ccaccgggcc aggtacttaa
    13561 tccaggtctt tgatggagcg gacttcgtcc ttgttgaggc gagtcaactt gccgtcgggc
    13621 tgtttgaact cgtagaaccc gctgtcttcg tcaaactccg gggtgtccag ggactgcagc
    13681 tcgcgcccat cctttagggt gatggcggca ggcgtggaac agcccgccag caggccgatg
    13741 cacagcatga gggtggcgcc catgacagga tgcttgcgca tgatgagtta ccttcttgag
    13801 agagcatgaa aagtgtagtc agcctatggc aggcacacag actcgcccgt gtgcctgggc
    13861 gacagctggc tttggtcatg aatctcagtg ggcggctcct tgctccggcg ccttgctgcc
    13921 cgcgatgaca gactgttgat ccaccggtcc aatccagctc gccactcgcg cgcaggcatc
    13981 tcggcacccc atcccgctgg cgaatcatcc gtacaccgtc actccccatg ctccttcaaa
    14041 tgcctgacac ccgcggacca tgacgtggcg cgccttgcca ctgccacagg agcaaccgcc
    14101 ccatgtttag gtttattctg acctgaagac ttcgtcattc tgtacattct gcgtcgacca
    14161 aactctatgc tgtcagcgga atgcgcctct ctgatcggga aacaagggat gaaacagcaa
    14221 cagaaatgga tcggccgcgg cgtgttgctg gcggccctga tcggtggcgg ctggctcgtc
    14281 tggcagggat tgcaacccaa ggggctgggt gatggcttcg tcagcggcaa cgggcgcatc
    14341 gaggccaccg agatcgacgt ggccaccaag ctggccgggc gcatcgcgga gatcggcgtc
    14401 gacgagggcg attttgtcca gcccggacag gtgctcgccc gcatggacac cgaggtactg
    14461 caggcccagc tgcgccaggc cgaggcccag gtgcgccagg cgcagaacgc catcctcacc
    14521 gcccgcgcca tggtcgcgca gcgcgaaagc gagaaggcca ccgccgaggc ggtggtgctg
    14581 cagcgccagg ccgagctgac cgccgcgcag aaacgccacc agcgcaccga gacgctggtc
    14641 acgcgcaacg ccgcgtcgcg ccagcagctc gacgacgatc tggccgcacg cctcggcgcc
    14701 caggcggcgg tgacggcggc gcgcgccaag gtgctgtccg accaggccgc catcgaggcg
    14761 gccaaatccc aggtgatcga agccgaatcc accgtcgagg cggcgcaggc gaccgcagct
    14821 cgcctgcagg ccgacatcaa cgacagcgcg ctgaagaccg accgggtagc ccgcgtgcag
    14881 taccgcatag cgcaggccgg cgaggtgctc ggcgccggcg gcaagctgct caacctggtc
    14941 gatctggccg acgtctacat gaccttcttc ctccccgagc gccaggccgg ccgcgtggcg
    15001 ctgggcagcg aggtgcgcct ggttctcgac gccgcgccgc agttcgtgat cccggccaag
    15061 gtcagctacg tcgccagcgt cgcccagttc acgccgaaga ccgtggaaac cgccagcgag
    15121 cgggagaaga tgatgttccg ggtcaaggca cgcatcgatc ccgaactgtt gcgcaagtac
    15181 caggatcagg tgaagaccgg cctgccgggc atggcctacc tcaagctcga tcctgaggcc
    15241 gaatggccca cccacctgca gttgaatgcc cagcaatgag tgaggcaccc gtcgcccggc
    15301 tggacaacgt cagtctcagc tacggcaaga cccaagccct cgacggtgtc agcctggagg
    15361 tcccggcccg gcgcatggtc ggcctgatcg gcccggacgg ggtcggcaag tccagcctgc
    15421 tggcgctggt cgccggcgcc cggcagatcc agcaaggcag cgtgcaggtg ctggatggcg
    15481 acatggcttc gcgtcatcac cgtcgcgtcg tgtgcccgcg catcgcctac atgccgcagg
    15541 gcctgggcaa gaacctctac ccgacgctga cggtgttcga gaacctcgag ttcttcggcc
    15601 gcctgttcgg ccaggcccgt gaggagcggc gctggcggat cgacggactg acccgcagca
    15661 ccggcctgca tcccttcctc gaccgcccgg cgggccagtt gtccggcggc atgaaacaga
    15721 agctcggcct gtgctgcgcg ctgatccacg atcccgacct gctgatcctc gacgagccga
    15781 ccaccggcgt cgatccgctg tcgcgcaacc agttctggga gctgatcgag cacatccgcg
    15841 ccgaccgccc acagatgagc gtgctggtgg ccaccgccta catggacgag gcgcagcgct
    15901 tcgaccatct ggtggcgatg gacgccggcc gcgtgctcgc caccggcacg ccggccgaac
    15961 tgctggcacg caccgccagc gccagcctgg aggaagcctt catccgcctg ctgccggagg
    16021 agaaacgccg ccagcaccgc gagctggtga tcacgccgcg ccagcacaac gacagcatcg
    16081 ccatcgaggc ccacggtctg accatgcgct tcggcgattt cgtcgcggtc gaccaggtga
    16141 actttcgcat cgaacgtggg gagatcttcg gctttctcgg ctccaacggc tgcggcaagt
    16201 cgaccaccat gaagatgctc accggcctgc tgccggccag cgagggcgag gccttcctgt
    16261 tcggcaagcc ggtggacccg cacgacatgc agacccgcca gcgggtcggc tatatgtcgc
    16321 agtccttctc gctgtacagc gaactgacgg tgctgcagaa cctcgacctg catgcccgcc
    16381 tgttccacat ccctgccgag cgccgcgccg cgcgcatcgg cgagatgctc gagcgcttcg
    16441 gtctgagcgc agaggccgac agcctgccca ccagcctgcc gctgggcgtg cgccagcgcc
    16501 tgtcgctggc ggtggcggtg atccacaacc cggaaatcct catcctcgac gagcccacct
    16561 cgggggtcga ccccatcgcc cgcgacggct tctgggaact gctggtgcag ctgtcgagac
    16621 aggatggggt gaccatcttc atctccaccc acttcatgaa cgaggcgctg cgttgcgacc
    16681 gcatctcgct gatgcacgca ggccgggtgc tggacagcga cacgccgcag gcgctgatgg
    16741 acaagcgcgg cctgccgagc ctggaagcca ccttcatcgc ctatctcgag gaggctgccg
    16801 cggagaacgc ggccagcctg ccggcacccg ccagctcgcc tgcccccgtg gcagcggcac
    16861 ccgcggcccc cggccgcacg gcagaccggc aatcgcgttt cagcctgcgc cgcctgctca
    16921 gctacagccg ccgcgagtcg atggaactgc gccgcgaccc gatccgcgcc accatggcca
    16981 tgctcggcac ggtcatcctg atgttcatca tgggctacgg catcagcttc gacgtggaga
    17041 acctgagcta cgcggtgctc gaccgcgacc agaccagcac cagccagagc tatctgctca
    17101 acatgtccgg ctcgcgctat ttcatcgagc aggcgccgct ggccagccat gccgagctgg
    17161 accagcgcct gcgcagcggc gacatcagcc tggcggtgga gatcccgccg aacttcgccc
    17221 gcgacctcaa gcgcggcagc cccccgcagg tggccttctg gatcgacggc gccatgccga
    17281 tgcgcgccga caccatcaag ggctacgtgc agggcatcca cctcagctac ctgcagcaac
    17341 tggcccgcga ggccggcgtc gacaatctcg gcgcgcccac cgaggtcgcc gtgcgctacc
    17401 gctacaaccc cgacgtgcag agcctgccgg cgatgatgcc ggcgatgatc ccgctgctgc
    17461 tgatgatgat cccggcgatg ctcaccgccc tcggcgtggt gcgcgagaag gagctgggct
    17521 cgatcaccaa cttctacgtc accccgacca cccgcctgga gttcctgatc ggcaagcagc
    17581 tgccctacat cgccctgggc atggtcaact tcgccatgat ggtgctgctg gcggtgtacc
    17641 tcttcggcat cccggtcaag ggcagcctgc tgaccctgac cttcggcgcc ctgctctata
    17701 tcgcctgcgc caccggcctc ggcctgctga tgtcgtcgat cctcaacagc cagatcgccg
    17761 ccatcttcgg caccaccatc gccaccctgc tgccggccat ccagttctcc ggactgaccc
    17821 acccggtatc ggcgctggag ggcgccagcg ccttcatcgg ccagctgtat cccaccagcc
    17881 atttcctgat catcagccgc ggggtgttct ccaaggcgct gaacctggcc gatctctacc
    17941 cgtacttcat ccccatgctg ctgaccattc cggtgctgac cctgctcagc atcgccgggc
    18001 tgcgcaagca ggagaaatga gatgagcgcc agccgccgca agctcgccaa catcttccag
    18061 ctcggcctca aggagctgcg cagcctgtac cgcgatccgg cgctgctgat cctgatcatc
    18121 tggtcgttca ccatggcggt ctactccagc gccacggcca tcccggaagc tccgcaccgc
    18181 gccagcatcg cggtggtcga cgaggaccag tcgcaagcct cgcggcgcat catcaacgcc
    18241 ttccagctgc cctacttcat ccagcctgcc gccatcgacc tggcgcagat ggacagcggc
    18301 atggatgccg gcctgtacac cttcaccctg aacattccgc cgggcttcca agaggacctg
    18361 ctggccggcc gccagccgac catccagctc aacgtcgacg ccacccaagt cagccaggcg
    18421 ttcaccggcg ccgggcatat ccagcagatc atcgccaccg agacggccga gttcgcccag
    18481 cgctatcgca gcagcgaggc cctgcccatc gaggcggtgg tgcgcaccgc gttcaacccc
    18541 aacctgaccc gcgcctggtt cggcgcggtg aacgaggtga tcaaccagat caccatcctg
    18601 gcgatcatcc tcaccggtgc ggcactgatt cgcgagcgcg agcacggcac catcgagcac
    18661 ctgctggtga tgccggtcac cccgttcgag atcatgctcg gcaaggtctg gtcgatgggc
    18721 ctggtggtgc tggcggcgtc ggccttcgcc ctgcgcttcg tggtcgaggg ctggctggac
    18781 atcccgctgc agggctcgac gctgctgttc accggcggcg ccgccctgca cctgttcgcc
    18841 gtcacctcga tgggcatctt cttcgccacc gtggcccgct ccatgccgca actgggcctg
    18901 ctgaccatcc tgacgctgat gccgctgcag atcctctccg gcggcaccac cccgcgcgag
    18961 agcatgcccg agctggtgca gaacgtgatg ctggccgccc cgaccaccca cttcgtcgaa
    19021 ctggcgcagg ccatcctgtt ccgcggcgcc gacctgaaca tcgtctggcc gcaactgctg
    19081 gcgctggcta ccatcggcag catgttcttc atcggcgccc tgtcgcgcct gcgcaagtcg
    19141 ctgcgctaac ggatacttcc aatgatgcgc tccaccgcca acaccacaag gaggcacgcc
    19201 ttgaacagga cccccggtac gctgcgcaag cttgccctgg ccatcgcgct cagcagcgcc
    19261 cccaccctgc tgctggccga accagccgca ccgtcgccgc gggccgacct gatcggcgtc
    19321 taccagcagg cgctggagaa caatgccgac ctcgccgcgg cccgcgccga ctacctggcg
    19381 cgcaaggaag tagtcccgca ggcacgctcg ggcctgctgc cgcagctcaa cggcggcgcc
    19441 agcctcggcg atacgaagac cgaactggac cagccctcgg tcagcctcga gcgcagcggc
    19501 accctctacc aggccagcct cagccaggcg ctgttccgcg ccgaccgctg gttccagctg
    19561 caggccgcca aggcggtcag cgaacaggcg gccctgcagt tctcctccgt cgagcagaat
    19621 ctggtcctgc agagcgccga agcctacttc gccgtcctgc gcgcccagga tggcttggcc
    19681 gcgagccgag ccgaggaagc ggcgctcaag cgccactacg accaggccaa cgatcgcttc
    19741 gaggtcggcc tgtccgaccg caccgaagtg ctcgaggcgc agtccagcta cgacaccgcg
    19801 cgcgccaacc gcatcctcgc cgaacgcctg gtggaagacg ccttccaggc gctcgccacc
    19861 ctgaccaatc gcgactacag cttcatcgaa ggcatccgcc ataccctgcc cgtcagcccg
    19921 ccggcgccca acgacgccaa ggcctgggtc gataccgcga tgcagcggaa cctcgacctg
    19981 caggccagcc agtacgcggt gaccggtgcc gaggaaaccc tgcgccagcg caaggccggc
    20041 cacgcgccga ccgtggacct ggtggcgcag taccagaaag gcgacaacga ccggctgggc
    20101 ttcaccaata ccgccctggc cgggcggatc ggcagtacca tcagcggcga cgtcgaacag
    20161 cagagcatcg gcgtggagct gaacatcccg ctgtacagcg gcggcctgac caactcgcag
    20221 gtgcgcgagt cgtaccagcg cctgaaccag accgagcagc tgcgcgaaag cctgcgccgc
    20281 caggtggtgc agaacacccg cgacttccac cgcgcggtga acaccgacgt ggcgcaggtg
    20341 gcggcccgca gacaggccat cgtctccagc cagagcgccc tggaggcgac cgagctcggc
    20401 tacgaggtcg gcacccgcaa catcgtcgac gtactcgacg cccagcgcca gctctacctg
    20461 gccgtgcgcc tgtacaacaa cacgcgctac gactacatcc tcaaccacct gcgcctgaag
    20521 cgctccgccg gcattctcag cccggccgac ctgcaggacc tggagagctt cctcaagccc
    20581 gactacaacc ccgaccagga cttcctgccc ccgggcctgg cgccgagcct gagcggccgc
    20641 caaggcgagt gagcaaacgg ccggcgcgtt acgcccggcc gaacagctcg gcgacgccgg
    20701 gttggcgaga cccctgcttt gcgcagcggg ggctgagcca tccgagcagc ccatttccca
    20761 tcacttgccg cggctttacg ggtgataatg cgccgattgt ttcccgctca atgacggagt
    20821 cccgcggcgt gatgttcgag atcaagcagt tagatccaga gacctaccgg cggcagacgc
    20881 ggcgcagcac gctgatcatc gtgatgatct tcgcgctgct cgccatgctg ctgtccagca
    20941 tggccgtgct gctgttcggc gaagccggtg gcgataattt ccgctggaac atgggcggcg
    21001 tgattgccgg cctgctactg accggggcgc tggtgcggct gcacttctgg tcgcagccct
    21061 ggatggcacc ggccgtttac ggctggcagc tcaagcgcag cctgatgagc gttaccaacg
    21121 tcatgcacca cgtcaccgcc ggcgtgcagg cccgcgatcc ggccgccatg aaactgctgc
    21181 gtttctatca tcttggactg acccagatgc accagctgga tgccaactcc agcgccctca
    21241 gccagatggt gcgggagatc aaccagcacc gcgagtgcat ggaggccttg cacatggaca
    21301 ccgaccagta tcgattggac cctgcctggc tcgaagcggt gaagaaggcc ggcgcatagc
    21361 gcccgatacg ggccgcatgc gccgcaatgt cccaagtccc ggtcagtcgt attccgcttc
    21421 ctggtgctcg cccagcaggc gccgttgccg tggcgtggcc gcggccagca cggcgggatt
    21481 gaactgccag tgcaggtacg gcgagaactt ctgcgccacc atgcgccggc catagtgcac
    21541 ctgcagcagg tagcgcgtgc ggtctggggt ccggttgtcg ctaccggcgt gccacagttc
    21601 gctgcgaaac atcagcacgt cgccggcccg gcacagcacc ggctgcgcgg tgcggccctg
    21661 ccactggctt tcgccggcca tgggcgctcg cccggcgcgg tggctgccgg ggatcactcg
    21721 ggtggggctg aggtcggcat cgatatcgtc gaggtagagg tgtgcggtgc agatctgcat
    21781 cggcaacgcg aaggatgggt cggccaacag gctcggtggc agctccatca ccaggtagtc
    21841 cagatgcagc tcggcgccga cgaagccggg atggctgcgc caggccgtct ggccgatgat
    21901 gtggcaatcg gccccagggc ggcctcggcc agctcgatga tcccgcccag gtcgagatac
    21961 ggcagccaga gcggatcgcg gttgaatacg cacttgtaat ggtcgcacag gccgctgtag
    22021 tcccagtgca taggctgcag gctgtcgatc gcctggcgca gcatggcgat ttgcgccgca
    22081 gcgagaaccc cggggagcag gacgaaaccg tcctggtgca aggcccgcag acggctggcg
    22141 gtcagggctg acaagggttc ggtgctggcc atgcggtgct cccggcggac gctgatgacg
    22201 ccacgtcata ctggcattgc gtggggggct cagccagtgc ggcatcgcaa acagcttcaa
    22261 tcgtcgcgcg ccgcccgcgc atgatcggca aattcgctga acaccccgtc cacccccagc
    22321 gtgtagaaca gccggtattc cgccccgggg tcgcccttga agtcggaggc cagccgtttg
    22381 gcttcgctgc ggaaggtata ggcgtgcacg aacagcccgg cggcatgggc gttcctgacc
    22441 acctcggtgg gcggcatcat cacccggtcg cgctcgtcga gctggccgtc gccgtttagg
    22501 tcgtccggct ggccatcgtg gttatcatcc agcagccgtg aaggaatgag atagggcttc
    22561 caggggccga tgccgtcagc gtaggtcttg acctctttca ggccggcagg cgtcagcagg
    22621 ctggcgaagg tgcgggcatc gccggccacc gcaaagtcgt agggcttgtc aaagggtgcg
    22681 gccagctgga tgctgccatc ggcattgata tcgctggcgt ccaccagttg caccaggcgc
    22741 agctgcgtct tgccgcgcag gtactgcaga ttcgacacct cgaacgactg caggatcacc
    22801 ggcgaggcct tgctggtgta gccgtagcgg gccagggtat cgagcaggcg atcttcgagc
    22861 ttgagcccca gacgggcgtg ataggtcgga tgcttggtct cggcatacac gccaatgctg
    22921 cgccccaggc gcgccccttc gcttctggcc aggtcgagca cctcatcgaa ggtgggaatc
    22981 tgcaacttgc cgttgtagga ctgatcgcgg tcggcaaagg gctggatggc gcgcagggtc
    23041 ttgagttcgg cgagcgtgaa gtccgtgacg aaccagcctt cttcctcgac accatcgacc
    23101 gtccgccgcg tcttgcggct ggcgaattcg gggcgacccc tgacgtcggt ggtgctggtg
    23161 atgttcggct catggcgcgc caccagcacg ccatcccggg tagccaccag gtcgggctcg
    23221 atgaagtcgg cgcccagctc gatggctttc cggtagccct ccagcgtgtg gtccggcagg
    23281 tagccggcgg cgccgcggtg gccgaccacc aggggcttgt cgccgttcag cgtggggtat
    23341 ttcgccccgc cgcgatcacc gccgtaggcc gtcagcgcca gagcggcacc gaggaccagc
    23401 gtcaccgttt ttgcatcgcg ccgggtcata tctcacctcg ttctatcgct gcgggggcag
    23461 acaaccgacc ttggttccca ggttggtgcc ctacagccta gccagtattc ggcccatggc
    23521 tattcagaac atcgagccga cgattcagtg catcagcggc ggtagtcatc ccctggatgt
    23581 cacctccgtg gaagatgagg atcagcagga gccatggccc ccccaaaggg cccgtatgct
    23641 ggtttgtcag ctgcgagtta agacgatctg ctaatgctgg atctggcgaa tgagcccgag
    23701 gacttcacgt cagttcgtga agtcgtgcac tccaggtggt ctgggtgtgc aggtcgatca
    23761 agctgagcga gccgttctcg aagcagttac cggtgtcgat gaacgtggtg ttgccgagct
    23821 ggaggatggc ccgcacccgg ctatggccga cgtagatctg gtcgatgccc gtcaccagcg
    23881 aggtgtcctg ctgttccagt cgcgccttgt tccacatggc gtcgccgatg acggtcatgc
    23941 gctccagcag tccatcggta ttgccgcgag tgcgctcgag cggggcgctg agtacctcgc
    24001 ggaagcgctg ccagtcggac aacgggcatt cgccgtgcac gaggccgaag cggagctggt
    24061 tgcgcccttc gatctcgaag gcgatcggca gttgccggag ccgctcgctg acatcctgcc
    24121 ggtgccggct ccccagctga tagaaccagc cacccccatg catcatgtgc tgctcggtgg
    24181 agaagccact ggcgccatcc acgcacatct ggtcgtgatt gccgcgcacg gagtggaacc
    24241 agggctcatc gagcagcgcc agcacctgca gcgactgcgg gccgcgatcc accaggtcgc
    24301 cggcagcgaa gacgcgatcc acccgacggt cgaagccggc cttggtcagc aggtgctcaa
    24361 gcagcgaggc ctggccgtgg atgtcaccca ccagcaggtc gcgcccccgg tcattgcgag
    24421 cgaaggtggc gaacaacccg ctgggtgcca ggtcgagata ggggatggcc atgatgatgc
    24481 tgtccttggt tggcgccggt tacagggtgg tcagggatga ataatgaacc tctactggcg
    24541 aaaggagcca ggcggctcgc gcgccagggt atcggtccac cgaggcacat cggcccgtcg
    24601 acttctggta acttcgcggc tatcgcatcg cgaagaagac agaagaacaa caatggcttt
    24661 cccgttagtc aacccgagtc agctgcgctc accctcgctc acggacaaga ccctggcgcg
    24721 accgcgcctg ctgcagcgcc tgcaagagtc cgccagccgt ggctgccgcc tgaccttgct
    24781 cagcgctccg ctcggctacg gcaagagcac cctgctcgcc cagtacgcgg cgagcctgca
    24841 gacgccctgg gcatggtatc ggttggacaa cgccgacaac caaccgttgc acctgctgct
    24901 gcacctgcat gccgccctcg gcctggccac acccgagcgc gacctggtgg cggccgacga
    24961 gccgcgcctg tggagcgcca tcctccagcg actggagcac gcggcggacc acttcaccct
    25021 gatcctcgac gacctgcacc tgctgcgcgc cggccgtgcc cgccactacc tcgagcagtt
    25081 gctgcgcttc gggccgcccg gcctgcacct gctggccgcc tgcgaaggcc agccggaact
    25141 ggccctcagc cacctgcgtc gcgaggagca gctgaccgtc ctcgatgccc gcgagctggc
    25201 cctggacagc gaggaaaccc gccagttggc caacagccgc ggcgccaagc tgggcaccga
    25261 tgccctctac tacgagctgc gcgccaacag cgaaggctgg atcagcggcc tgctgctctg
    25321 gctcaccgcc taccgcgagc agcctccggc cagccagtcg cgcagggacc tgcgcaacgt
    25381 gacccggcag gcctacgcct atgtgacgcg cttcctcgaa gaagagctgc tcgctcagct
    25441 cccttccgag ctgctggcct tcctcgaaag caccgcggtg gtgcagacct tcgacccgac
    25501 gctggcgggc atcttgagcg gccagccgaa tgccgaacgg ctgattcgtc aactgctgcg
    25561 ccgcgacctg ttcatcgagc agcgccccgg cgagcgcgtg gaataccgct accatgcggt
    25621 cctccgcggc gtgctctacc agcgcctgca gcagcgcgat ccgcagttgc tgcagcagtt
    25681 gcaccagcag gctgccgcct ggctgctgga gcagcgccgc tactctgagg ccatccatca
    25741 atacggccgg gccagggact tcaacgcggt gctcgccatc gccgaacggc acaccttcga
    25801 cctgctgcgc gatgggcaga tcaacgcgct ggtcgacctg ctcagcgagg tttccgggca
    25861 gaacagcagc gatcacttca ccctggccat cagcgaagcc tccatcgtgt tggcgaccaa
    25921 cgacatcgcc agcgcgcagg catgcatccg gcgccttcag cgccaaaatg tgccgcgcca
    25981 ccccgagcgg gtccaacaga ccatcgccta cctgcgtacc cttctcgcgt tcctcggcgg
    26041 caacctgcgc catggcctgg aactggcgag ccgggcgctg cagagatatc cgcagcgcaa
    26101 caccgccggc tctgtgctgc gcttcaaccg agcccgatgc ctgttccacc ttggcctgct
    26161 tggcgaggca cgcctcgacg tgacacagac tctggaggag ctcaacgact tcggtttcag
    26221 cggcttcgcc aacattcccc acctgttgct ggggcagatc gaactggctc aaggtgacag
    26281 cgacgcggct atgaagcgct tcctggcgct ggagcgagaa tcgccggcca ccgcctcgcg
    26341 taacttctac gagctgttcc accatctggg cctggggcta gtgttcgtcc agcacaatcg
    26401 tttggagcag gcccggcagt gtctggcaaa agccgaagct ctagcgctca gctttcccca
    26461 tacggccgga ctggttcggg tcctccatta ccaagcttgc ctgttcgacg cactgggcga
    26521 cagcaacatg gcacaggccc gctgggacga ggtccggcgc atggcccgcc agttccgcca
    26581 gttcggtctc tatcgcctgg ctggtgcctg gcgtgtccgc ctggccgtgc gcgagcgcga
    26641 cgaggacttc atcctgcaat ggcttcagga atggcactgg tgccagcgcc actacggcga
    26701 ggatctcttg ccggaggaat ggctggccta tgcttgggtg cagcgccatc tcggtcaacc
    26761 ggctgtggcc ggacagatcc tagacaactt gcgcgagcag gccgagagcg aaggcaaccg
    26821 tcgtctgcag ctggatctgt ggctactcga cgctaccctg cggcgcgacc atggcgatca
    26881 aatggcagcc ttggcgtgcc tggacgatgc cctgcatctg gccgttagct acagcctcgg
    26941 ccagatgctg caccacgagg gccgcgagct ggccgagctg ttccgccagc tgctgctgcc
    27001 acaggtgcgc cggcatgcgg ggctagaacg tccactaccg tcccgcgatc tgctggccgc
    27061 agcattaacc ggactcgtta gcgatagcac catgcatatg ctgcttgaac ccatcacccg
    27121 ccgcgagttg gacgtgctgc ggcgtatggc gcgtggacag aacaaccagc agcttgccga
    27181 cggtctgttc gtcagcctca gtacggtgaa gacccacatc aacaacttgt tccggaagct
    27241 cgatgtcacc gatcgcgacg ctgcgctgcg cgctgcccga gagctgcaac tgctcgactg
    27301 agtaaccgtg tcccgagtca agcggttttg aactcgggat tccaaggcag tccgctcctg
    27361 gccatggcat tgagcgtggt caggagcttg cgcatgcagg caacgagcgc gaccttgggc
    27421 agcttgccgg cggcca
//
LOCUS       DATCUN010000195         4089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_271291, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000195 DATCUN010000000
VERSION     DATCUN010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271291"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1434
                     /locus_tag="VIO83_07890"
     CDS             223..1434
                     /locus_tag="VIO83_07890"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY8331231.1"
                     /translation="MQVSKSNKLANVCYDIRGPVLKHAKRLEDEGHRILKLNIGNPAP
                     FGFEAPEEILQDVIRNLPISQGYSDSKGLFSARKAVMQYCQQKNIEGVTIEDIYLGNG
                     VSELIVMALQALLNNGDEVLIPAPDYPLWTAATSLAGGKAVHYLCDEQSNWYPDLDDI
                     KAKITRNTKALLLINPNNPTGAVYPKEVLEQIIEVARQHNLVIFSDEIYDKILYDGAE
                     HISTASLAPDVLCLTFNGLSKAYRVAGFRSGWVIISGPKHKARGYIEGLDILANMRLC
                     ANVPAQHAIQTALGGYQSINDLVLPHGRLYEQRNRTWELLNDIPGVSCVKPMGALYAF
                     PKIDPKVCPIHNDEKFVLDLLLQEKLLIVQGTAFNWPWPDHFRVVTLPRVDDLELAIG
                     RIGNFIKGYRQ"
     gene            1481..1912
                     /locus_tag="VIO83_07895"
     CDS             1481..1912
                     /locus_tag="VIO83_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331232.1"
                     /translation="MDLQIDDFYQDASRALLALYRAFPRKTTLYIDDLIGYHEPDEYG
                     LPAVRHQSCLGTLLWLGEEGYLRHAGTLSHEAVEQVVLSEKGFLRLSTRIADVQLQHA
                     DLPENALRVRSTLARQLQESLASGDGERLANLARQLLNPDA"
     gene            2028..2903
                     /gene="htpX"
                     /locus_tag="VIO83_07900"
     CDS             2028..2903
                     /gene="htpX"
                     /locus_tag="VIO83_07900"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEY8331233.1"
                     /translation="MMRILLFLATNLAVLVIASITLKLLGVDRFTGQNYGSLLVYCAV
                     FGFAGSLISLFISKWMAKMSTGTEIISQPRTRHEQWLLQTVEQLSRDAGIKMPEVGIF
                     PAYEANAFATGWNKNDALVAVSQGLLERFSPDEVKAVLAHEIGHVANGDMVTLSLIQG
                     VVNTFVMFFARIFGNFVDKVILKNEEGPGIGYFVATIFAELVLGILASIIVMWFSRKR
                     EFRADESGADLAGTGAMIAALQRLRAEQGVPVHMPESMTAFGINGGLKHGLAGLLMTH
                     PPLEDRIEALRQRGH"
     gene            3008..3655
                     /locus_tag="VIO83_07905"
     CDS             3008..3655
                     /locus_tag="VIO83_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HEY8331234.1"
                     /translation="MRPPLTLVFATLLLLTGCQSAYYSAMEKVGVHKRDILADRVEEA
                     RDAQVEAKEQFRDALERYRSVVQVNGGELEERYNALSSEYAASEDSAKAVHQRIAAVE
                     DVAEALFDEWEGELAQYSNASLRSASARELARTRQDYRTLLQRMKTAERRIEPVLSVL
                     HDQVLFLKHNLNARAIGSLQGEYRTLQGNVDQLLRDMQRAIDESDAFVRRLQSSN"
     gene            complement(3699..>4089)
                     /locus_tag="VIO83_07910"
     CDS             complement(3699..>4089)
                     /locus_tag="VIO83_07910"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156222.1"
                     /note="catalyzes the S-adenosylmethionine-dependent
                     transmethylation of thiopurine compounds; may be involved
                     in selenium cycling by forming dimethylselenide and/or
                     dimethyldiselenide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HEY8331235.1"
                     /translation="ALRRYQAQGIAVLQGDFFAVTPAQTAGCAGYYDRAALIALPPEM
                     RRRYVAQLQALLPSGCRGLLVTIDYPQEQKDGPPFAVSDAEVRALYGEGWQIELLEDT
                     DVLADNAKFGAQAVTELRQRVYRLLRR"
BASE COUNT          777 a         1454 c         1201 g          657 t
ORIGIN      
        1 ctctccgaca actcctcgcg ccactcttcc aggggctttt cgaccttttg catggaattc
       61 acctcgccgt ggaaaaaagc ccgcccgttc cctttaaccg ggctcgaggc ggtcgtatca
      121 tgcgtgcctt tcagtctgtc acccgtgttt gcacctcaca agccatccac agggcttgcg
      181 ccggaaacga atgcgggccg ctggttagat cggggtccca tcatgcaggt cagcaaatcc
      241 aacaagctcg ccaacgtctg ctacgacatc cgcgggccgg tactcaagca cgccaagcgc
      301 ctcgaagatg aaggccaccg catcctcaaa ctgaacatcg gcaaccccgc cccgttcggt
      361 ttcgaggcgc cggaggaaat cctgcaggac gtgatccgca acctgccgat ctcccagggc
      421 tacagtgact ccaagggtct gttcagtgcg cgcaaggcgg tgatgcagta ctgccagcag
      481 aagaacatcg aaggcgtcac catcgaggac atctacctcg gcaacggcgt gtccgagctg
      541 atcgtcatgg ccctgcaggc gctgctcaac aacggcgacg aggtgctgat cccggcaccc
      601 gactacccgc tgtggaccgc ggccaccagc ctggccggcg gcaaggcggt gcactacctg
      661 tgcgacgagc agtcgaactg gtaccccgac cttgacgaca tcaaggccaa gatcacccgc
      721 aacaccaagg cgctgctgct gatcaacccg aacaacccca ccggcgcggt gtacccgaag
      781 gaagtgctgg agcagatcat cgaggtcgcc cgccagcaca acctggtgat cttctccgac
      841 gagatctacg acaagatcct ctacgacggt gccgagcaca tctccaccgc ctcgctggcg
      901 ccggacgtgc tgtgtctgac cttcaacggc ctgtccaagg cctaccgggt agccggtttc
      961 cgctccggct gggtgatcat ctccggaccc aagcacaagg cgcgcggcta catcgaaggc
     1021 ctggacatcc tcgccaacat gcgcctgtgc gccaacgtgc cagcccagca tgcgatccag
     1081 accgcactcg gtggctacca gagtatcaac gacctggtcc tgccccatgg ccgcctctac
     1141 gagcagcgca accgcacctg ggagctgctc aacgatatcc ccggggtcag ctgtgtcaag
     1201 ccgatgggcg ccctctatgc cttcccgaag atcgacccca aggtctgccc gatccacaac
     1261 gacgagaagt tcgtgctcga cctgctgctg caggaaaagc tgctgattgt ccagggcacc
     1321 gccttcaact ggccgtggcc ggaccacttc cgcgtggtca ccctgccacg ggtcgacgac
     1381 ctcgagctgg ccatcggccg catcggcaac ttcatcaagg gctaccgcca gtaacctgcc
     1441 gatctgccgc cctgtggggc ggcagatgcg agtccccagc atggatctac agatcgacga
     1501 cttctaccag gacgccagcc gcgccctgct cgccctctat cgggcgtttc cgcgcaagac
     1561 caccctctac atcgacgacc tgatcggcta ccacgagccc gacgagtacg gcctgcccgc
     1621 cgtccgccac cagagctgcc tgggcaccct gctgtggctc ggcgaggaag gctacctgcg
     1681 ccatgccggc accctcagcc acgaggccgt cgagcaggtc gtgctgagcg agaaaggctt
     1741 cctgcgcctg tccacccgca ttgccgatgt gcaactgcaa cacgccgacc tgcccgagaa
     1801 cgcgctgcgg gtccgcagca cgctggcccg ccagctgcag gaaagcctcg ccagcggcga
     1861 cggcgaacgc ctggccaatc tggcccgcca gctgctcaat cccgacgcct agccggcagg
     1921 cgacacagtt tgaaatagcg cctgggttgt aataaccccg cgcgctccct tatatagccg
     1981 ctcagactca tcgcgactct gccgcaagca aggagacggt tttcatcatg atgcgtattc
     2041 tgctgttcct ggccaccaac ctggccgtac tggtaattgc cagcatcacc ctcaagctgc
     2101 tcggagtcga ccgtttcacc ggccagaact acggcagcct gctggtctac tgcgccgtgt
     2161 tcggctttgc cggctcgctg atctcgctgt tcatctccaa gtggatggcg aagatgagta
     2221 ccggcaccga aatcatcagc cagccacgca cccgccacga acagtggctg ctgcagaccg
     2281 tcgagcagct gtcgcgcgat gccgggatca agatgcccga ggtcggcatc ttccccgcct
     2341 acgaggccaa cgccttcgcc accggctgga acaagaacga tgcgctggtc gcggtcagcc
     2401 agggcctgct cgaacgcttc tcgccggacg aagtgaaggc cgtgctggcc cacgagatcg
     2461 gccacgtggc caacggcgac atggtcaccc tgtcgctgat ccagggcgtg gtgaacacct
     2521 tcgtgatgtt cttcgcacgc atcttcggca acttcgtcga caaggtgatc ctcaagaacg
     2581 aggaaggccc gggcatcggc tacttcgtgg cgaccatctt cgccgagctg gtgctgggca
     2641 tcctcgccag catcatcgtc atgtggttct cgcgcaaacg cgagttccgc gccgacgagt
     2701 ccggcgccga cctggcgggt accggggcga tgatcgccgc gctgcagcgc ctgcgtgccg
     2761 aacagggcgt gccggtacac atgccggaaa gcatgaccgc cttcggcatc aacggcggcc
     2821 tcaagcatgg cctggccggc ctgctgatga cccacccgcc gctggaagac cgcatcgagg
     2881 ccctgcgcca gcgcggtcac tgattgccaa gcgggtcgaa gttttacccc gggacagggc
     2941 gacgcaagtc gcccttttcc tgtccgctgg ttaccatgcc cccgttatcc ctcgcgagaa
     3001 gctgccgatg cgcccacctc tgaccctcgt gttcgccacc ctgctcctgc tgaccggctg
     3061 ccagagcgcc tactacagcg ccatggaaaa ggtcggcgta cacaagcgcg acatcctcgc
     3121 cgaccgtgtc gaggaagcgc gcgacgccca ggtggaagcc aaggagcagt tccgcgacgc
     3181 gctggagcgc taccgcagcg tggtgcaggt caatggcggt gagctggagg aacgctacaa
     3241 cgcgctcagc agcgaatacg ctgccagcga ggacagcgcc aaggccgtgc atcagcgcat
     3301 cgccgccgtc gaggacgtgg ccgaagcgct gttcgacgaa tgggaaggcg agcttgcgca
     3361 gtacagcaac gccagcctgc gcagcgccag tgcccgcgag ctggcccgca cccgccagga
     3421 ctaccgtacg ctgctccagc gcatgaagac tgccgaacgg cgcatcgagc cggtactcag
     3481 cgtgctgcac gaccaggtgc tgttcctcaa gcacaacctc aacgcccgcg cgatcggctc
     3541 gctgcagggc gagtaccgca ccctgcaggg caacgtcgac cagctgctgc gcgacatgca
     3601 gcgcgccatc gacgagtccg atgccttcgt gcggcgcctg cagagcagca actgacgacc
     3661 agcacggccc gtcgcggccc cggcgctcgc caaagcgact agcgacgcag cagccgatac
     3721 acccgctggc gtagctcagt caccgcctgc gcgccgaact tggcgttgtc cgccagcaca
     3781 tcggtgtcct ccagcagctc gatctgccag ccttcgccat acagcgcgcg cacctcggca
     3841 tcgctgaccg cgaacggcgg gccgtccttc tgctcctgcg gataatcgat ggtcaccagc
     3901 agcccacggc agccactcgg cagcagcgcc tgcagctgcg ccacgtagcg acggcgcatc
     3961 tccggcggca gggcgatcag cgccgcgcgg tcgtaatagc cggcacaacc cgccgtctgc
     4021 gccggcgtga cggcgaagaa gtcgccctgc agcacggcaa tgccctgcgc ctgatacctg
     4081 cgcaaggcg
//
LOCUS       DATCUN010000196         4583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_82941, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000196 DATCUN010000000
VERSION     DATCUN010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82941"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..140)
                     /locus_tag="VIO83_07915"
                     /pseudo
     CDS             complement(1..140)
                     /locus_tag="VIO83_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014720463.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
     gene            complement(179..1180)
                     /locus_tag="VIO83_07920"
     CDS             complement(179..1180)
                     /locus_tag="VIO83_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007986225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8331236.1"
                     /translation="MRISVPGRLGFGTAPLGNMYRNIPEQEALDTVEAAWQQGIRYFD
                     AAPMYGAGLAEIRLGTALQQHRRDDYVLSTKVGRLILDEREDTTQRDLGEKGGLFEHG
                     LPNKIVYDYTADGTLRAIEASLARLKTDRLDIVWIHDPARDFHGEGWRDVFDIAMNGA
                     TKALTRLREEGVISAWGLGVNRVEPCEMALQQADPDGFLIAGRYTLLDHADALRTLMP
                     ASTARGLGIVVGGPYNSGILAGGTHYEYQQATPDILARVDALRAVCAEFNVDIRAAAL
                     QFSLAHPAVAAVIPGASQPSRIAENLALVDAEIPAAFWQALRTRGLVAVDAPLPQTA"
     gene            complement(1220..2221)
                     /locus_tag="VIO83_07925"
     CDS             complement(1220..2221)
                     /locus_tag="VIO83_07925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017339934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03571 family LLM class oxidoreductase"
                     /protein_id="HEY8331237.1"
                     /translation="MSPASHASNASALSRLTAGGFSIGVEAPLDNDWTPAGELARRNS
                     GRLPGEPDMQRHAALAKLVDQLGFRALWVRDVPVYDPSFGDAAQVFEVFSYLGYLAGI
                     THDILLGTAAVVLPIREPLLTLKAAATVQQLSGNRLLMGVASGDRPVEYPLFGRDFPG
                     RGANFRNQVDLLRDHARAHLPEPLDLLPRPSTPLPLLVAGLSQQTPAWIGQHMDGSLA
                     FPGTPAEHAHRVAAWRAVAGAKPYASFIHLELDARPDAPMKRWRFGFSGGRTALIQEL
                     QAMRAAGVDHIGLHVRRNQRPLDDTFHEIAQYVLPLFHTCTATDNAPGAATRATADT"
     gene            2570..3022
                     /locus_tag="VIO83_07930"
     CDS             2570..3022
                     /locus_tag="VIO83_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY8331238.1"
                     /translation="MKVKYVETRAKGGKIINLVVTSRLAHRTLDYCQTTKNKAAATAD
                     GKTARVLLNSRGRPVTSKSYSPAFERVADRLGLSATPHMSRYTVATNMKSRLEQMEKQ
                     SANINPIKVIQHLLAHSSAEPTELYLASANSIDAGTLRAILETEEALG"
     gene            complement(3214..3444)
                     /locus_tag="VIO83_07935"
                     /pseudo
     CDS             complement(3214..3444)
                     /locus_tag="VIO83_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611954.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3821..4042
                     /locus_tag="VIO83_07940"
     CDS             3821..4042
                     /locus_tag="VIO83_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331239.1"
                     /translation="MIDRMKFELPGKPAYWIAAGILCALTGLGMDMGMSMSGTVLSVS
                     EPAKWGGFGALIGTSVGLMAGFGKGSAQR"
     gene            complement(4405..4583)
                     /locus_tag="VIO83_07945"
                     /pseudo
     CDS             complement(4405..4583)
                     /locus_tag="VIO83_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014340837.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          816 a         1404 c         1562 g          801 t
ORIGIN      
        1 agccgggccg ccgcaagcgc ggcgggcagc acgccgttca ccccgcgccg ttcgccggtc
       61 agcgccagct cgcccagaaa ttcttagcgc gacaacacct gcgtatcgat ctgcccgctg
      121 gcggcgaaga tgcccagcgc gccgcgctac ggcgatggca ctacgcccac ccttcgcctt
      181 acgccgtttg cggcagcggc gcatccaccg ccaccaaccc gcgcgtgcgc agtgcctgcc
      241 agaacgcagc cggaatctcc gcatccacca gcgccagatt ctcggcgatg cggctcggct
      301 ggctggcgcc gggaatcacc gccgccacgg ccggatgcgc cagcgagaac tgcagggcgg
      361 ccgcgcggat gtccacgttg aactcggcgc acaccgcacg cagggcgtcc acgcgggcga
      421 ggatgtcggg cgtggcctgc tggtattcgt aatgcgtgcc gccggccagg atgccggagt
      481 tgtacgggcc gcccaccacg atgcccaggc cgcgcgcggt gcttgccggc atcagggtgc
      541 gcagtgcgtc ggcgtggtcc agcagggtgt accggccggc gatcaggaag ccgtccgggt
      601 cggcctgctg cagcgccatt tcgcacggct cgacgcggtt gacgcccagc ccccaggcgc
      661 tgatgacgcc ttcctcgcgt agccgggtca gcgccttggt cgcgccgttc atggcgatgt
      721 cgaatacgtc gcgccagcct tcgccgtgga agtcgcgggc cgggtcgtga atccacacga
      781 tgtccaggcg gtcggtcttc aggcgcgcca ggctcgcctc gatggcgcgc agcgtgccgt
      841 cggcggtgta gtcgtagacg atcttgttgg gcaggccgtg ctcgaacagg ccgcccttct
      901 cgcccaggtc gcgctgggtg gtgtcttcgc gctcatcgag gatcaagcgc cccaccttcg
      961 tgctgagtac gtagtcgtcg cggcggtgtt gctgcagcgc cgtgcccagc cggatctcgg
     1021 caaggccagc gccgtacatg ggggcggcgt cgaagtagcg gatgccctgt tgccaggcgg
     1081 cctcaacagt gtcgagggct tcctgctcgg ggatgttgcg gtacatgttg cctagcgggg
     1141 cggtgccaaa gcccagccgg ccgggaacgg aaatgcgcat gggtgtctcg atgaaggggg
     1201 agggggaaag cggtcgtgct caggtgtctg cggttgcgcg cgttgccgcg ccgggcgcgt
     1261 tgtcggtggc cgtgcaggtg tggaacagcg gcaggacgta ctgggcgatt tcgtggaagg
     1321 tgtcatccag cgggcgctgg ttgcggcgca cgtgcaggcc gatgtggtcc acaccggcag
     1381 ctcgcatggc ctgcagctcc tggatcagcg cggtgcggcc gccactgaag ccgaagcgcc
     1441 agcgcttcat gggcgcgtcg gggcgcgcgt ccagctccag gtggatgaag ctggcatagg
     1501 gtttggcacc ggccaccgcg cgccacgcgg ccacgcgatg tgcgtgctct gccggcgtgc
     1561 cggggaaggc caggctgcca tccatgtgct ggccgatcca cgcaggggtt tgctgcgaca
     1621 gcccggccac cagcagcggc agcggcgtgg acgggcgggg cagcaggtcc agcggttcgg
     1681 gcaggtgcgc gcgcgcgtgg tcgcgcagca ggtcaacctg gttgcggaag ttggcgcccc
     1741 gaccggggaa gtcgcggccg aacagggggt attccaccgg gcggtcaccg ctggccacgc
     1801 ccatcagcaa ccggttgccg ctgagctgct gcacggtggc ggcggctttg agggtcagca
     1861 gcggctcgcg gatgggcagc accacggcgg cggtgcccag caggatgtcg tgggtgatgc
     1921 cggccaggta gccaaggtag ctgaacacct cgaacacctg cgcggcatcg ccgaaggacg
     1981 ggtcgtacac cggcacgtcg cgcacccaca acgcccggaa gccgagttgg tccaccagct
     2041 tggccagggc cgcatggcgc tgcatgtccg gttcgccggg cagccggcca ctgttccgtc
     2101 gggcgagctc accagcgggg gtccagtcgt tgtccagcgg cgcttccaca ccgatgctga
     2161 agccgccggc ggtgaggcgg gagagggcgc tggcgttgga tgcgtgcgag gcgggggaca
     2221 tggggagatc cagtgagagg ccggtggtac gggtggccga cagtctggtg gctggatctt
     2281 tgcggagtaa acggggaatg cgggaatttc ttttgcttgg agttcaacaa tcaacgaagg
     2341 gggaagctgc gatccagccg atgcggtggg gacgggtatc gctcgaacta gccggctgcg
     2401 gcgagcaaaa acttacaaga gaaaagtgcg tgccatgagc ccggaggaga cttcgcttat
     2461 ggctgccatc tgaaggtgcc ttactccctg acgttccttt ggactcttgg cacgggccct
     2521 cgtcgcacta ccgtcgcaga cctcgctctg acacagtccg aagaccatcc tgaaggttaa
     2581 gtatgtcgag acgcgggcaa agggtgggaa gatcatcaac ctggtggtga cctcccgctt
     2641 ggcgcatcgt acgctcgact actgccagac aactaaaaac aaggctgctg caacggcaga
     2701 cgggaaaacc gctcgcgttc ttctgaactc aaggggcagg ccagtcacat ccaagtctta
     2761 ctccccagcc tttgaacgag ttgccgatcg tctcggtctg agcgcgacac ctcacatgtc
     2821 gcgatacacc gtcgctacga acatgaagag tcgtcttgag cagatggaaa agcagagcgc
     2881 aaacatcaac ccgatcaagg taatccagca tcttcttgca cacagtagtg cagagccaac
     2941 tgagctttac ctggcctccg ccaactccat tgacgcaggc acacttaggg cgattctaga
     3001 gacagaagag gcgctgggat gactgaggtt tcagtagccc agttcgccgg cgacttcccc
     3061 ggagatcctg tcgacagcta cgacttctcc cccttaggcc ttggaaccaa gcctacgtag
     3121 gcattgctgc tcgctctcac tggagtcacc tctacctaca ggctgcagag tcgaaagcaa
     3181 gcttgggggg cgcttcgaaa gttcgtgaaa ttcctaatcc agttggatgg cgatgcttgg
     3241 aagaatatgc caccctttac tggcgatcct gcgccgcatt ctcgggctgt cgcgccgctc
     3301 gatgtgatcg aacaaatctg ctggtgctcc atccaacgtg aggtggttgg gccgacagat
     3361 ccgctaacgc ttgagcattc caaagaattc ttcgcaggta gcgttgtcgc cacaatggcc
     3421 cactgtgctc attgagtgga gaacctgacc cgcgcgaact cagtgaactt gcggcgctga
     3481 tcgccgactg cctgagcaac gtagcctgta gcctcgtcca aagcactggc cgaggcatcg
     3541 gcgttcatgc gacagtcgat ggcgaatcat cgcatcgccg acctacaggg gcgcctgatg
     3601 cgcggattga acgagggctt cgagcaggac tgagcggagg tgtttactcc gcgttagcgg
     3661 ctaccgctgg accatgcttg cgtcgggcga atgcttcgag cggggctgtc tagagcgaag
     3721 cagcctgagt gcaggcaggc tgagagcaag cagttcgcga agggcgcgcc gcatgcaagg
     3781 ccacaaccgt atgtattcct acagaaggga ggcaagaaaa atgatcgatc ggatgaagtt
     3841 cgaactaccc ggcaagccgg cctactggat tgccgccggc atactctgcg ctctgaccgg
     3901 cctgggcatg gacatgggca tgagcatgag cgggaccgtg ctgtcagtga gcgagccggc
     3961 caagtggggc ggtttcggag cactgatcgg aaccagcgtc ggtctgatgg cggggtttgg
     4021 caagggttcc gcgcaacgct gaccgcacaa cgcctagccg ccgcgtgtgc gacgggtggc
     4081 gttgattcgc aacgagcatt gagtcatggt cgtcgccagt aagtggcggc accgcctcgt
     4141 cggtgctcag ctgcttgatg ctcagctcgt cgacccgtat gtgccgacgt acaagctgac
     4201 caccgcgcag ctgggcgccc tcattagcgg ttcttgcgcg cacatcgccg ggttgcagct
     4261 gcatggcgga caaaatgatt cgcacgcaat ccttctgtgt tcggatggat gcgagtcggc
     4321 tcgttggtca ggctgggagc cagctgaaca gatatgcaac tttctcgacg gcgctgctga
     4381 cagacaaatt tacgacctaa aagcgagttc acctaggacc agcacgccgt tgtgcgccag
     4441 cgatatctcg cccgggcgcg gaaacgagcc gccgcccacc agcgataccg cgctggcggt
     4501 gtggtgcggt gagcggtacg ggcgctgccg ccaacgcgcg gtgtcgatgc cgccgccggt
     4561 gcaggacgcc accgtggcgg tct
//
LOCUS       DATCUN010000197         6417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_139319, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000197 DATCUN010000000
VERSION     DATCUN010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139319"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VIO83_07950"
     CDS             complement(<1..303)
                     /locus_tag="VIO83_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY8331240.1"
                     /translation="MSVFTPLERHELEAFLAPYGLGRLLEFEGIAAGTENSNFFVSLE
                     HGEYVLTLIERGPREDMGFFIALLDVLHAAGLPVPYAVRAQDGQALGELAGLPALLQ"
     gene            complement(440..724)
                     /locus_tag="VIO83_07955"
     CDS             complement(440..724)
                     /locus_tag="VIO83_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HEY8331241.1"
                     /translation="MRAINRLLLAGLLAAAPLAAIAQDEAPSAEPDVTIRQEGDKTIQ
                     EYRVNGFLYAIKITPKVGKPYFLVRADGDSNFIRSDKPDMLIPQWEIFRW"
     gene            802..3567
                     /gene="polA"
                     /locus_tag="VIO83_07960"
     CDS             802..3567
                     /gene="polA"
                     /locus_tag="VIO83_07960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY8331242.1"
                     /translation="MSLAPLVLVDGSSYLYRAFHALPPLHTSRGLPTGAVKGVLNMLL
                     SLRRQYPDSPFAVVFDAKGPTFRDELFEHYKSHRPPMPDDLRVQIEPLHACVRALGLP
                     LLCIEGVEADDVIGTLARSSAAAERPVVISTGDKDMAQLVNGHIILVNTMTNPPTYMD
                     SDGVTAKFGIGPELIIDYLALMGDKVDNIPGVPGVGEKTALGLLQGLGGGLDVIYANL
                     EQVPALPIRGAKSLPAKLEEHREMAYLSYQLATIKTDVALELSLDQLHPSAPDRETLI
                     PLYRELEFKNWLDDLLREAKAADLAAPAANAAENAAPTPAAPEQHYETVLEPAQFDAW
                     LAKLQAAELFAFDTETTGLDAQQAELVGISFAVSAHEAAYVPLAHSYMGVPEQLDRDA
                     VLAALKPLLEDPARAKVGQHAKYDINILARYGIEVRGVAFDTMLESYVLDSTATRHDM
                     DSLALKYLGQSTVKFEDIAGKGAKQLTFDQIALEQAGPYAAEDADVTLRLHQALWSKL
                     EAIPSLAKVLTEIEMPLVPVLARIERHGALVDARLLGQQSLELGEKMQQLQRAAFELA
                     GEEFNLASPKQLGAILYDKLGLPTLSKTAKGQASTAESVLAELAEQGYALPQVLMDYR
                     SVSKLKSTYTDKLPEQINPHTGRIHTSYHQAVTATGRLSSSDPNLQNIPIRTAEGRRI
                     RQAFIAAPGYRLVAADYSQIELRIMAHLAQDEGLLDAFRHDRDVHRATAAEVFGVELE
                     QVTTEQRRSAKAINFGLIYGMSAFGLAKQIGVDRKQAQAYIDRYFARYPGVLAYMERT
                     RAQAAEQGYVETLFGRRLYLPEIHAKNGAMRKAAERTAINAPMQGTAADIIKHAMVAV
                     DAWLPASGLDARMILQVHDELVLEVREDQVEALKAGLLPLMSGAAQLDVPLLVEAGVG
                     SNWDEAH"
     gene            complement(3791..4441)
                     /gene="yihA"
                     /locus_tag="VIO83_07965"
     CDS             complement(3791..4441)
                     /gene="yihA"
                     /locus_tag="VIO83_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY8331243.1"
                     /translation="MFVKNPILGLCQQASFIKSAAQVDQCPPDEGCEVAFAGRSNAGK
                     SSAMNTLTHARLARTSKTPGRTQLLNFFRLDDQRRLVDLPGYGYAKVPIPLKQHWQRH
                     LEAYLGSRHSLVGVVLLMDIRHPLTEFDCLMLDWTKASCLPMHILLTKADKLTYGAAK
                     NALLKVQKDIRQGWGEVASVQLFSAPKRQGIEEAHAVLAHWMGLLGAEDAEAAADA"
     gene            4662..4958
                     /locus_tag="VIO83_07970"
     CDS             4662..4958
                     /locus_tag="VIO83_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8331244.1"
                     /translation="MKKLLLAVSLVAATFSVQAAQDPEAVFNRACGVCHMGAIPTAPV
                     KGDKAAWEPRLAKGMDALVASVTNGFNAMPPRGMCMDCTAEDYKAIIQWMSTPK"
     gene            5004..5609
                     /locus_tag="VIO83_07975"
     CDS             5004..5609
                     /locus_tag="VIO83_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8331245.1"
                     /translation="MNKLLVSLLLTLGVTGLAQAAGDATAGQTKAAVCGACHGPDGNS
                     PAPTFPKLAGQGERYLLKQMKDIKSGARPVVPMTGLLDNLSDQDLADISAYFASQKGS
                     VGQADPKLVARGEALFRGGNLTAGMPACSGCHSPNGEGNDLAGFPQLGGQHAAYVATQ
                     LTNFREGVRTNDGDTMIMRSIAAKLSNKDIEAVSSYIQGLH"
     gene            5761..6405
                     /locus_tag="VIO83_07980"
     CDS             5761..6405
                     /locus_tag="VIO83_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEY8331246.1"
                     /translation="MRNLILSAALAGISLFGATAHAEDIQAGKQYVELTSAVPVAKPG
                     KIEVVELFWYGCPHCYQLEPTIQPWAAKLPEDVNFVRIPAMFGGVWNVHGQMFLTLEA
                     MGAEAKVHEAIFSAIHREGKKLATPEEMAEFLAGQGIDKDKFLSTFNSFAIKGQMEKA
                     KKLAMAYQITGVPVLVVNGKYRFDLGTAGGPGGAIQVADHLIAKERAAQPAPAQ"
BASE COUNT         1138 a         2217 c         2059 g         1003 t
ORIGIN      
        1 ctgcagcagc gccggcagcc cggccagctc gcccagcgcc tggccgtcct gggcgcgcac
       61 cgcgtagggc accggcaggc cggcggcgtg cagcacgtcg agcagggcga tgaagaagcc
      121 catgtcctcc cgcggcccgc gctcgatcag ggtcagcaca tactcgccat gctccaggct
      181 gacgaagaaa ttgctgttct ccgtgccggc ggcgatgccc tcgaactcca gcaggcggcc
      241 gagcccgtag ggcgcgagga aggcttccag ttcgtggcgt tccagcgggg tgaagacgga
      301 catgacgcct cctgcagtac gattggtgcg gtcggcaaaa cccagcgtag cggttctggc
      361 taggcgtgca ggtgcagaca gtacaagtgg tacggcaagc ctgcccaaca acgccagcag
      421 gcgttttgcc ggccgcgact taccagcgga agatttccca ctgcggaatc agcatgtccg
      481 gcttgtccga acgaatgaag ttgctgtcgc cgtcggcgcg caccaggaag tacggcttgc
      541 cgaccttcgg ggtgatcttg atggcgtaga ggaagccgtt gacgcggtat tcctggatgg
      601 ttttgtcgcc ctcctggcgg atggtcacgt ccggctcggc gctcggcgcc tcgtcctggg
      661 cgatggcggc aagcggagcg gcggccagga ggccggcgag cagcagacgg ttgattgcac
      721 gcatgatggt aaccttgtcc ctttgaagtg aatgaccccg tcatcttacc tcggtcgcat
      781 tgcccaagag tcgatttccc tatgagtcta gcccctctcg tcctggtgga cggttcctcc
      841 tacctgtacc gcgccttcca cgcgctgccg ccgctgcaca cctcgcgcgg cctacccacc
      901 ggggcggtga agggcgtgct gaacatgctg ctgagcctgc gccggcagta tccggacagc
      961 cccttcgcgg tggtgttcga tgccaagggg ccgaccttcc gcgacgagct gttcgagcac
     1021 tacaagtcgc accgcccgcc gatgcccgac gacctgcgcg tgcagatcga gccgctgcac
     1081 gcctgcgtgc gcgccctggg cctgccgctg ctgtgcatcg agggcgtgga ggccgacgac
     1141 gtgatcggca ccctggcgcg ctccagcgcg gcggccgagc gcccggtggt gatctccacc
     1201 ggcgacaagg acatggcgca gctggtcaac ggccacatca tcctggtcaa caccatgacc
     1261 aacccgccga cctatatgga cagcgacggc gtcacggcca agttcggcat cggccccgaa
     1321 ctgatcatcg actacctggc gctgatgggc gacaaggtcg acaacatccc cggcgtgccg
     1381 ggggtcggcg agaagaccgc gctgggcctg ctgcagggcc tgggcggcgg cctcgacgtg
     1441 atctacgcca acctcgaaca ggtgccagcg ctgccgatcc gcggcgccaa gtcgctgccg
     1501 gccaagctcg aggaacaccg cgagatggcc tacctgtcct accagctggc caccatcaag
     1561 accgacgtgg cgctggagct cagcctcgac cagctgcacc cgagcgcgcc ggaccgcgag
     1621 acgctgatcc cgctgtaccg cgagctggaa ttcaagaact ggctggacga cctgctgcgc
     1681 gaggccaagg ccgccgacct cgcggccccc gccgcgaacg ccgccgagaa cgcggcgcct
     1741 acacccgccg cgcccgagca gcactacgag accgtgctgg agcccgcgca gttcgacgcc
     1801 tggctggcca agctgcaggc cgccgagctg ttcgccttcg acaccgagac caccggcctc
     1861 gacgcccagc aggccgagct ggtcggcatt tccttcgccg tcagcgccca tgaggcggcc
     1921 tatgtgccgc tggcgcacag ctacatgggc gtgcccgagc agctcgaccg cgacgcggtg
     1981 ctggccgcgc tcaagccgct gctggaagac ccggccaggg ccaaggtcgg ccagcacgcc
     2041 aagtacgaca tcaacatcct cgcccgctac ggcatcgagg tgcgcggcgt ggccttcgac
     2101 accatgctgg aatcctacgt gctggactcc accgcgaccc gccacgacat ggacagcctg
     2161 gcgctgaagt acctcggcca gtcgacggtg aagttcgagg acatcgccgg caagggcgcc
     2221 aagcagctga ccttcgacca gatcgccctc gagcaggccg gtccctacgc cgccgaggac
     2281 gccgacgtca ccctgcgcct gcaccaggcc ctgtggagca agctggaggc cattccctcg
     2341 ctggcgaaag tgctgaccga gatcgagatg ccgctggtgc cggtgctggc gcgcatcgag
     2401 cgccacggcg cgctggtcga cgcccgactg ctcggccagc agagcctgga gctgggcgag
     2461 aagatgcagc agctgcagcg cgcggccttc gagctggctg gcgaggagtt caacctcgcc
     2521 tcgcccaagc agctcggcgc catcctctac gacaagctcg gcctgccgac cctgtcgaag
     2581 accgccaagg gccaggcctc gaccgcggaa agcgtgctcg ccgagctggc cgagcagggc
     2641 tacgcgctgc cacaggtgct gatggactac cgcagcgtca gcaagctcaa aagcacctac
     2701 accgacaagc tgccggagca gatcaatccg cacaccgggc gcatccacac cagctaccac
     2761 caggcggtga ccgccaccgg ccggctgtcc tcctcggacc cgaacctgca gaacatcccg
     2821 atccgcaccg ccgagggccg gcgcatccgc caggccttca tcgccgcgcc ggggtaccgg
     2881 ctggtggccg ccgactactc gcagatcgag ctgcgcatca tggcccacct ggcccaggac
     2941 gagggcctgc tcgacgcctt ccgccacgac cgcgacgtgc accgcgccac cgccgccgag
     3001 gtgttcggcg tcgagctgga acaggtgacc accgagcagc ggcgcagcgc caaggcgatc
     3061 aacttcggcc tgatctacgg catgagcgcc ttcggcctgg ccaagcagat cggcgtcgac
     3121 cgcaagcagg cgcaggccta catcgaccgc tacttcgccc gctatcccgg cgtgctggcc
     3181 tatatggagc gcacccgcgc ccaggccgcc gagcagggct acgtggagac gctgttcggc
     3241 cgccgcctgt acctgccgga gatccacgcc aagaatggcg ccatgcgcaa ggctgccgag
     3301 cgcacggcga tcaacgcgcc gatgcagggc accgcggcgg acatcatcaa gcatgcgatg
     3361 gtggcggtgg acgcctggct gccggcgtcc gggctggatg cgcggatgat cctccaggtg
     3421 cacgacgaac tggtgctgga ggtgcgcgag gatcaggtcg aggcgctcaa ggccggcctg
     3481 ctgccgctga tgagcggcgc cgcccagctg gacgtgccgc tgctggtgga ggccggggtg
     3541 ggcagcaact gggacgaggc gcactgacac ggccggcgct gctcgccagc gccgccagaa
     3601 agccgaccga cggttgaact ttcgccagga cacccactcg aattgcatga gtggttggca
     3661 gacctctctg tggattgttc ttgttctatt cacactgaga atccggatcc cccgccctgg
     3721 cgatccggta ttgaaacccg gacttccatc cccccaacgg aagtccggtt ttttttgccc
     3781 gccagaagcc tcaggcgtcc gccgctgcct cggcgtcttc cgcccccagc aagcccatcc
     3841 agtgggccag caccgcgtgc gcctcctcga tcccctggcg cttgggcgcg gagaacagct
     3901 gcacgctggc cacctcgccc cagccctggc ggatgtcctt ctgcaccttg agcagcgcgt
     3961 tcttcgccgc accgtaggtc agcttgtcgg ccttggtcag caggatgtgc atgggcaggc
     4021 agctggcctt ggtccagtcg agcatcaggc agtcgaattc ggtcagcgga tgacggatgt
     4081 ccatcagcag gaccacgccg accaggctgt ggcggctgcc cagataggcc tccaggtggc
     4141 gctgccagtg ctgcttgagc gggatcggca ccttggcgta gccgtagccg ggcaggtcga
     4201 ccaggcggcg ctggtcgtcg agacggaaga agttgagcag ctgggtccgc cccggggtct
     4261 tcgaggtgcg cgccaggcgg gcatgggtca gggtgttcat cgcactggac ttgccggcgt
     4321 tggaacgccc ggcgaaggcc acttcgcagc cctcgtccgg cgggcactgg tcgacctgcg
     4381 cggcgctctt gatgaaggag gcctgctgac acagaccgag gatgggattc ttgacgaaca
     4441 tggcgtgggc ctgtgctgcg cctgcacata ggcatggcag gctgggactg ggggtgaact
     4501 gcgaccaaat gtcgcgagcg gcggttccgt ttccgttttg acgtcgcctg tatataatgc
     4561 cacggatttt gtgtgcgctt tgttctcaag ttagtgccca tgcggacgga ccccgccagc
     4621 agaatgccgg gccgtttccc cctgatgaaa tgaggtcgac catgaagaaa ctgttgcttg
     4681 ctgtgtcgct cgtcgccgcc accttctcgg tgcaggctgc ccaggatccg gaagcggtct
     4741 tcaatcgtgc ttgcggcgtc tgccatatgg gtgccatccc cacggcaccg gtcaagggcg
     4801 acaaggccgc ctgggaaccg cgtctggcca agggcatgga cgcactggtt gcaagcgtca
     4861 ccaatggttt caatgccatg ccgccacgcg gcatgtgcat ggactgcacc gccgaggact
     4921 acaaggccat catccagtgg atgagcacgc ccaagtaagc ccgacacacc cttttccccc
     4981 cttagccgta gttggattag ctgatgaaca aactactcgt gagtctgctg ttgaccctgg
     5041 gcgtgactgg cctggctcaa gccgctggtg atgccactgc cggacaaacc aaggcagccg
     5101 tctgcggcgc ctgccacggt cctgacggca acagcccggc accgaccttc ccgaagctgg
     5161 ctggccaggg tgaacgctat ctgctcaagc agatgaagga catcaagagt ggcgcgcgcc
     5221 cggtagtgcc gatgaccggc ctgctggaca acctgagcga tcaggacctg gccgacatct
     5281 ccgcctactt cgccagccag aagggcagcg tcggtcaggc cgatcccaag ctggtggcgc
     5341 gcggcgaagc actgttccgc ggcggcaacc tcaccgccgg catgccggcc tgcagcggct
     5401 gccactcgcc gaacggcgaa ggcaacgacc tggccggctt cccgcaactg ggcggccagc
     5461 acgccgccta cgtggccacg cagctgacca acttccgcga aggcgtgcgt accaacgacg
     5521 gcgacaccat gatcatgcgc tccatcgccg ccaagctgag caacaaggac atcgaagcgg
     5581 tgtccagcta tatccagggc ctgcactaag cacccctgca gtagcctgca acacaaaaaa
     5641 gggtggccat ggccaccctt ttttttgccc gcctcgccta caatcgcgtg gaatccgtcg
     5701 accgcctcgc tgtccaatca gccgtgcggc aaaccacttc atcctgttca ggagtcaagc
     5761 atgcgtaacc tgattctctc ggccgcgctg gccggcatca gcctgttcgg cgccaccgcc
     5821 cacgccgagg acatccaggc cggcaagcag tacgtcgaac tgaccagcgc ggtaccggta
     5881 gccaagcccg gcaagatcga agtggtcgag ctgttctggt acggctgccc gcactgctac
     5941 cagctcgagc cgaccatcca gccgtgggcc gcgaagctgc cggaagacgt caacttcgtg
     6001 cgcatcccgg ccatgttcgg cggcgtgtgg aacgtccacg gccagatgtt cctgaccctc
     6061 gaggcgatgg gcgcggaagc caaggtgcat gaagccatct tctcggccat ccaccgcgaa
     6121 ggcaagaagc tcgctacccc ggaggaaatg gctgaattcc tcgccggcca gggcatcgac
     6181 aaggacaagt tcctcagcac cttcaactcc tttgccatca agggccagat ggagaaggcc
     6241 aagaagctgg ccatggccta ccagatcacc ggcgtgccgg tgctggtggt caacggcaag
     6301 taccgcttcg acctcggcac cgccggcggc ccgggcggcg ccatccaggt cgccgaccac
     6361 ctgatcgcca aggagcgcgc cgcccagccc gccccggccc agtaagcggc ggccgcc
//
LOCUS       DATCUN010000198         3375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_253378, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000198 DATCUN010000000
VERSION     DATCUN010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253378"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VIO83_07985"
     CDS             complement(<1..664)
                     /locus_tag="VIO83_07985"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8331247.1"
                     /translation="MRLVPNSLHDQVAATLREQIFAGSLTPGSFLDEAALCERLEISR
                     TPLREALKVLTAEGLLRHEPRRGCFVAEVTQRDLDEIFPVIALLEGRCAWEAARNASD
                     ADIEALATLHAQLEAHAKAHRINAYYATNFAIHEAIITLADNRWLAQVIGDLRKILKL
                     ARLQQLKAPGRLEQSLAEHLAVFAALKAHDSDGAEAAMRTHLHRQREALRAVALQQKS
                     RLV"
     gene            complement(1033..1494)
                     /locus_tag="VIO83_07990"
     CDS             complement(1033..1494)
                     /locus_tag="VIO83_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331248.1"
                     /translation="MRTYLEDFSALCRRSLLSLGPHQRGIGIQSFPHGTCGVVTALTG
                     RLLYEMTGEQGAHVCGTGHPDLGALQSHAWLEVRGYIVDLTHDQFRGTGMTGWVAQAS
                     DWHAKFDREVYPFRRAPRGALYANEAYVAMKSAASHLCLRPSRRVGASTVG"
     gene            1751..1945
                     /locus_tag="VIO83_07995"
     CDS             1751..1945
                     /locus_tag="VIO83_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331249.1"
                     /translation="MFNGSTVASQDAARWYELTDQYAAAVVAVQRGDAAARVRMLAMY
                     AELKRFAQSQGQPFPRGLGT"
     gene            2210..3028
                     /locus_tag="VIO83_08000"
     CDS             2210..3028
                     /locus_tag="VIO83_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY8331250.1"
                     /translation="MLDFFVERFPAVTEVSWRDRMAAGDVVDDAGHPIRPDAPYRGHQ
                     RLYYYRTLANEPRIPYEAQVLFQDAHLLVADKPHFLPVTPGGQYVQETLLVRLKRQLG
                     IDTLVPVHRIDRSTAGLVLFSLDPGTRGAYQALFAARRVEKSYEAVVHWPAGHTLAAV
                     YRSRLESIKGKMPVWEAPGEPNSETRFELLEMLGADRARLRLSPVTGRTHQLRVHCAA
                     LGMPIVNDPIYPTWLPVGSERFDQPLQLLARTLEFTDPLSGATRRFASELRLAG"
     gene            3104..3334
                     /locus_tag="VIO83_08005"
     CDS             3104..3334
                     /locus_tag="VIO83_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331251.1"
                     /translation="MNRAHAFSFLPLLRVLPLACVLAGCGTPAYEVVTYPNGATRTPS
                     TADAVGYCTQKGLTAQILERAPGQTGVMFRCI"
BASE COUNT          543 a         1169 c         1124 g          539 t
ORIGIN      
        1 aaacgagcct cgatttctgt tggagcgcca cggcgcgcaa ggcttcgcgc tggcgatgga
       61 ggtgggtgcg catggcggcc tcggcgccat cgctgtcgtg cgccttcagc gcagcgaaca
      121 cggccaggtg ctcggccagg ctctgctcga ggcggccggg cgccttcagc tgctgcaggc
      181 gcgcgagctt caatatcttg cgcaggtcgc cgatcacctg cgccagccag cggttgtcgg
      241 cgagcgtgat gatcgcctcg tggatggcga agttggtggc gtagtaggcg ttgatgcgat
      301 gcgccttggc gtgcgcctcg agctgcgcgt gcagcgtggc cagcgcctcg atgtcggcgt
      361 cgctcgcgtt gcgcgcggct tcccaggcgc agcgcccttc gagcagcgcg atgacgggaa
      421 agatctcgtc gaggtcgcgc tgcgtgacct cggccacgaa gcagccccgg cgcggctcgt
      481 ggcgcagcag cccttcggcc gtgagcacct tgagcgcctc gcgcaggggc gttcgcgaga
      541 tttccagccg ctcgcacagc gcggcttcgt ccaggaagct gccgggcgtg agcgacccgg
      601 cgaatatctg ctcccgcaag gtggcggcca cctggtcgtg cagcgaattc gggacgaggc
      661 gcatgagggc tccggtcgga ccttgaggca tcggacagcg gccaggccgt cggcgcccgc
      721 ctccgtaatt tcaggtaatt acagataatt atgggcaagg tgccccggac cgacaagggc
      781 ggaaaggggc ggaagggggc ggacaaaccc gctgtcggcg tggacggccc cgtcgccgtg
      841 tgtggccgga aagcgcgcaa aagaaggccc gcgccaggcg ggcccgaagg gtgggttctt
      901 gatgcccacc ctggagaaac agagttcagc gtagtcgcgg tgcccctgca gggcaagtcc
      961 ggtgcaagtt cgcttccggg ttcttcggtc tggcgcgggg gcgtgttttc ttcccgccgg
     1021 atggatgttt cctcagccca ccgtcgaagc gccgacccga cgcgaaggac gaaggcagag
     1081 atggctcgcc gcactcttca tggcgacata cgcctcgttg gcgtagagcg caccacgggg
     1141 ggcgcgccga aacggataca cctcccggtc gaacttggcg tgccagtcgg atgcctgcgc
     1201 cacccagccg gtcatcccgg tcccgcggaa ctggtcatgg gtcaggtcga cgatgtagcc
     1261 acgcacctcg agccacgcat gcgattgcag cgcccccagg tccggatgac ccgtaccgca
     1321 cacatgtgcg ccttgctcgc cggtcatctc atacagcagc cgcccggtca gcgccgtgac
     1381 cacgccacag gtgccgtgcg gaaaggactg gatgccgata ccgcgctgat gcggcccgag
     1441 cgacagaagg ctcctgcggc agagcgcact gaaatcttcg aggtacgttc tcatgaaccg
     1501 tcttcagcgc agggcgtccg gacccgccga agcaaaggga aaggcggggc atttttcaca
     1561 gctgcgcatt atcacgtcga ggcgctgccc ccttcctcgg aggccccgcc gacgcggcat
     1621 caccgcgtcg gcctaccaaa ataccgattc gtcacaataa cttagaaaga atgttgtaca
     1681 ttccgcttta agagggaaca cttaagttcg cgctgcggac agacagcgcg gatttccacg
     1741 gaggacagga atgttcaatg gttcgacggt ggcgtctcaa gatgcggcgc gttggtatga
     1801 gttgacggac caatacgcgg cggccgtggt ggcggtgcag cgaggcgacg ccgcggcgcg
     1861 agtgcggatg ttggccatgt atgccgaact caagcgcttt gcgcagagcc aggggcaacc
     1921 ctttccgaga ggtctcggga cctgacgagg tcgcttcgcg atcgctgccg ggcgcgatcg
     1981 ccctgccacc cgaccgtgag acgctggcgc gtggcatccg tgcatccgtg catgcgcgcc
     2041 cgatgcgccc caggcagcac gccaccggta cattcggggc accgtgcgcc gttccccatc
     2101 tccacccttt ccgccctctc cgcgcccttc gccctcccca ccgcccttgc ccacccgcga
     2161 tggcgtcggc cccagcaccg tcggattgcc ggccggccac tggccgacca tgctcgactt
     2221 cttcgtcgag cgcttcccgg ccgtgaccga agtgtcgtgg cgcgaccgca tggcggcggg
     2281 cgacgtggtc gacgacgccg gccacccgat ccggcccgac gcgccgtacc gcggccatca
     2341 gcgcctctac tactaccgca cgctcgccaa cgaaccgcgc atcccgtacg aagcgcaggt
     2401 gctgttccag gacgcgcacc tgctcgtcgc cgacaagccg cacttcctgc ccgtcacgcc
     2461 cggcggccag tacgtgcagg aaacgctgct ggtgcgcctc aaacgccagt tgggcatcga
     2521 cacgctggtg ccggtgcacc gcatcgaccg cagcaccgcc ggcctggtgc tcttcagcct
     2581 cgatcccggg acacgggggg cctaccaggc gctcttcgcg gcgcgccgcg tcgagaagtc
     2641 gtacgaggcg gtcgtgcact ggccggcggg gcacacgctg gccgccgtct accgcagccg
     2701 cctcgaaagc atcaagggca agatgccggt gtgggaagcg cccggcgagc cgaactcgga
     2761 aacgcgcttc gagctgctgg aaatgctggg cgccgaccgc gcgcggctgc gcctgtcgcc
     2821 ggtgaccggc cgcacgcacc agttgcgcgt gcactgcgcc gcgctgggca tgcccatcgt
     2881 caatgaccca atctacccga cgtggctgcc ggtcggcagc gagcgcttcg accagccgct
     2941 gcaactgctc gccaggacgc tggaattcac cgatccgttg agcggcgcga cacgccgctt
     3001 cgcgtcggag ctgcggctgg caggctgaac ggatccgggg ccggacgccg ttccttcacc
     3061 ggcggcgacc acccgtcgga ccgactgcgc cagaatcggc cggatgaacc gagctcacgc
     3121 cttctccttc ctccctcttc tgcgcgtcct gccgctcgcc tgcgttctcg ccggctgcgg
     3181 cacgccggct tacgaagtcg tcacctaccc caatggcgcg acgcgcacgc cgagcacggc
     3241 cgatgccgtg ggctattgca cgcagaaagg cctgacggca cagatcctgg agcgggcgcc
     3301 cggacagacc ggcgtcatgt tccgctgcat ctgaccgggc ctctcccacc gggtatcgga
     3361 gactcggcct ctcag
//
LOCUS       DATCUN010000199         4157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_139362, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000199 DATCUN010000000
VERSION     DATCUN010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139362"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2117
                     /gene="pgaA"
                     /locus_tag="VIO83_08010"
     CDS             <1..2117
                     /gene="pgaA"
                     /locus_tag="VIO83_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010565355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HEY8331252.1"
                     /translation="YRQALLREPNNTDLQLGLALTQADGGQASEAVQRTRALVAAKPD
                     DPNRRMALGYALTRAGLNYDALFEFDQAFIRAGDKPEVAREYIVALQKARLPEPALRL
                     ATQRPGLLDPVTQRRLEGDLAAERVRMAEFAARTEKERYVIADRALQDYDKLLTRWTP
                     DASAHDDVVRWRIDRLGALKARARTAEVIREYETLRAEGVQLPTYAVRWVAASYLDQR
                     QPEKAEPLYRQALSATDAEPDDRVEDSTALFYALQESDKGVEARKVATTLADNQKPRV
                     ELKGLPIGNPNDAWMDAQQLSAQSGVYGGDLPGSEAGLEALVARAPGNVGLRLAQADM
                     YRARDLPRRAEATLKETEAQVPRDISLEVSQAYTAMDLQEWRQLDVLADDVIARNPDS
                     RQVQRLQRLRNVHDMAEVRVEAYTGKSYGGGNNGDVGAVTGSRDWGIESVIYTPPIDE
                     DWRLFAGAGYATADFSEGTGQHRWQRVGVERRTRDMTLEAEVSNHSYGFGSKQGARVS
                     IARDINDNWQYGGSLDYLSATTPLRALNADITANGGSGFIRWRANESREWKLSLSPSH
                     FSDGNDRIETLLTGREGIYSSPGVQVDLGLEVSASHNSKADTVYFNPKSDFSVLPTVT
                     VNHVLYHRYETQWSQQFQVGAGTYSQRDYSTGGVGLIGYGQRFRWNDVLEAGANLSLI
                     SRPYDGDRERDLRLLVDLTYRF"
     gene            2130..4127
                     /gene="pgaB"
                     /locus_tag="VIO83_08015"
     CDS             2130..4127
                     /gene="pgaB"
                     /locus_tag="VIO83_08015"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HEY8331253.1"
                     /translation="MTVLSRCLLVLGVLLASACAQQPAPFTPPAERPTPANEVPWPKN
                     HFLGIAYHDVEDRDPDQAVVAVRTERLIEQLAWLRENGYQAVSVDQVLAARRGGPALP
                     PKAVMLSFDDGYSSFYTRVMPILRAYHWPALLAPVGTWIDTPMNQPVDFAGSPRPRGE
                     FLTWAQIREVSRSGLVEIAAHTDASHKGILANPQGNLEPAATSLRFDPATGRYETQAQ
                     FEARMRADVVAISNKIRSVTGKAPRVWVWPYGAAKGTSLAIVGEQGYEMALTLEDGLD
                     SSSNLMNSPRFLVASDPDGEHFANSIVAVQTQAPLRVLHVDLDNVYDPDPAQQARNLD
                     QLVQRVVDMGAGTVFLQAFADPKGDGLVHQLYFPNRHLPVRADLFNRVAWQLHTRAHA
                     SVYAWMPVLSFALDPKLPRVTRWDPETGKVGLDPDQYQRLSPFDPQVRKIIGEIYEDL
                     ARNNAIDGVLYHDDALLSDFEDASPAALKVYAANGLPASIAALRADPAVMQRWTRFKS
                     RYLIDFTQELTAKVRAIGGPQVQTARNIFAEPMLNPQSEAWFAQNLDDFLQTYDWTAP
                     MAMPLMEGETLKNSNAWLEKLVATVKARPGALERTVFELQAKDWRTKAAPDLNGAQLA
                     EWMGVLKRQGVTSFGYYPDNFLENSPDLKTVRPALSNQWNP"
BASE COUNT          787 a         1446 c         1275 g          649 t
ORIGIN      
        1 tgtatcgcca ggcattgctg cgtgaaccga acaataccga cctgcaactc ggcctggcgt
       61 tgacccaggc cgacggcggc caagccagcg aggccgtgca gcgcacccgc gcattggtcg
      121 cggccaagcc cgacgacccc aaccggcgca tggccctggg ctacgcgctg actcgcgccg
      181 gcttgaatta cgacgccctg tttgagttcg accaagcgtt tatccgcgcc ggcgacaagc
      241 ctgaagtcgc ccgtgaatac atcgtcgccc tgcaaaaggc ccgcctgccg gagccggcct
      301 tgcgtctggc gacccagcgc ccgggcctgc tcgaccccgt cacccagcgc cgcctcgaag
      361 gcgacctggc cgccgaacgg gtacgcatgg ccgagttcgc cgcgcgcacc gagaaagaac
      421 gctacgtgat cgccgaccgc gccctgcagg actacgacaa attgctcacc cgctggaccc
      481 ccgatgccag cgcccatgac gacgtggtgc gctggcgcat cgaccgcctg ggcgcactca
      541 aggcccgggc gcgcaccgcc gaggtgattc gcgagtacga aaccctcagg gccgaaggcg
      601 tgcaattgcc gacctacgca gtgcgctggg tggcggcgtc ctacctcgac cagcgccagc
      661 cggaaaaggc cgagccgctg tatcgccagg cactgagcgc caccgacgcc gagcccgatg
      721 accgggtcga agacagcacg gccttgttct acgccctgca ggaaagcgac aaaggtgtcg
      781 aagcccgcaa ggtcgccact accctcgccg acaaccagaa accgcgggtc gaactcaagg
      841 gtctgcccat cggcaacccc aacgatgcat ggatggatgc ccagcaactg tcggcgcagt
      901 ccggcgtgta cgggggcgat ctgccgggca gcgaagccgg cctggaagcc ctggtggcac
      961 gggctccggg caacgtcggc ctgcgcctgg cccaggcgga catgtaccgc gcccgcgacc
     1021 tgccacggcg cgccgaagcg accctcaagg aaaccgaagc ccaggtaccg cgcgacatca
     1081 gcctggaagt gtcccaggcc tacaccgcca tggatctgca ggagtggcgc caactcgacg
     1141 tgctcgccga tgacgtcatc gcccgcaacc ccgacagccg ccaggtgcag cgtctccagc
     1201 gcctgcgcaa cgtccacgac atggccgaag tgcgcgtcga ggcctacacc ggcaaaagct
     1261 atggcggcgg taataacggc gacgtgggcg cggtcaccgg cagccgtgac tggggcattg
     1321 aaagcgtgat ctacacgccg cccatcgatg aagactggcg cctgttcgcc ggcgctggct
     1381 acgccaccgc cgacttttcc gaaggcaccg gccagcaccg ttggcagcgc gtgggcgtgg
     1441 agcggcggac ccgcgacatg accctggaag ccgaggtgtc caaccactcc tacggctttg
     1501 gctccaaaca aggcgcgcgc gtgtcgatcg cccgcgacat caacgataac tggcaatacg
     1561 gcggcagcct cgattacctc tcggccacca caccgttgcg tgcgctgaat gccgacatca
     1621 cggccaacgg cggcagcggc ttcatccgct ggcgcgccaa tgaaagccgt gagtggaagc
     1681 tgtccctcag cccgtcccac ttcagcgacg gcaacgaccg catcgaaacc ttgctcactg
     1741 gccgcgaggg catctacagc tcgccgggcg tgcaagtgga cctgggcctg gaagtcagcg
     1801 ccagccacaa cagcaaggcc gacacggtgt acttcaaccc gaagtcggac ttcagcgtgt
     1861 tgcccaccgt caccgtcaac catgtgctct atcaccgcta cgagacccag tggagtcagc
     1921 agttccaagt cggagcgggt acgtatagcc agagggatta ttccaccggc ggtgtcggct
     1981 tgatcggtta cggccaacgc tttcgctgga acgacgttct cgaagccggc gccaacctga
     2041 gcctgatcag tcgaccctat gacggtgatc gcgaacgcga tctgcgcctg ctcgtcgacc
     2101 tcacttaccg tttctagaag agcctgacca tgactgtcct cagccgttgc ttgctggtcc
     2161 tgggtgtatt gctggccagt gcctgcgccc agcagcccgc ccctttcact ccacccgccg
     2221 agcggccgac cccggccaac gaagtgccgt ggccgaaaaa ccatttcctg ggcattgcct
     2281 atcacgacgt cgaggatcgc gaccccgacc aggcggtggt ggcggtgcgt accgaacgtt
     2341 tgatcgagca gttggcctgg ctgcgggaga acggttacca ggcggtcagt gtcgaccagg
     2401 tcctcgcggc ccgccgtggc ggtccggcgc tgccgccaaa agccgtgatg ctcagcttcg
     2461 atgatggcta ttcgagcttc tatacccgcg tgatgccgat actgcgggcc tatcactggc
     2521 cggccctgct tgcgccggtt ggcacctgga tcgacacgcc gatgaaccaa ccggtggact
     2581 ttgccggctc accgcgcccg cggggtgagt tcctgacctg ggcgcagatc cgcgaagtgt
     2641 ctcgctcggg tctggtggaa atcgccgcgc acaccgatgc cagtcacaaa gggatattgg
     2701 ccaaccccca gggcaacctg gagccggcag cgacctccct gcgcttcgat ccggccaccg
     2761 gccgctatga aacccaggcg cagttcgagg cgcggatgcg cgcggatgtc gtggcgatct
     2821 ccaacaagat ccgcagcgtc accggcaagg caccgcgggt gtgggtatgg ccctacggcg
     2881 cagccaaggg cacctcgctg gcgatcgtcg gcgagcaggg ctatgagatg gccctgaccc
     2941 tggaagacgg cctcgacagc tcgtccaacc tgatgaacag cccgcgcttc ctggtggcgt
     3001 ccgacccgga cggcgaacat ttcgccaaca gcatcgtcgc ggtgcaaacc caggcaccgc
     3061 tgcgggtgct gcatgtggac ctggacaacg tctacgaccc ggacccggcc caacaagcgc
     3121 gcaacctcga ccaactggtg cagcgcgtgg tggacatggg cgcgggcacc gtgttcctgc
     3181 aagccttcgc cgaccccaag ggtgatggcc tggtgcatca actgtacttc cccaaccgcc
     3241 acttgccggt gcgtgccgac ctgttcaacc gtgtcgcctg gcagttgcac acccgtgccc
     3301 atgccagcgt gtatgcctgg atgccggtgc tcagtttcgc cctcgacccc aagctgccgc
     3361 gcgtgacccg ctgggacccg gagaccggca aggtcggcct cgacccggat caataccagc
     3421 gcctgtcgcc gttcgacccg caggtgcgca agatcattgg cgagatctat gaggacctgg
     3481 cccgcaacaa tgccatcgac ggcgtgctgt accacgacga tgcgctgctg tccgacttcg
     3541 aagacgccag ccccgccgcg ctcaaggtct atgccgccaa cggcctgccc gccagcatcg
     3601 cggccctgcg cgccgacccg gcggtgatgc agcgttggac gcgcttcaag agtcgttacc
     3661 tgatcgactt cacccaggag ctgaccgcca aggtccgcgc catcggtggt ccgcaggtgc
     3721 agaccgcgcg caatatcttc gccgagccga tgctcaaccc gcagagcgaa gcgtggttcg
     3781 cgcagaacct cgacgacttc ctccagacct atgactggac cgcgccgatg gccatgccgc
     3841 tgatggaagg cgagacgctc aagaattcca atgcctggct ggaaaaactg gtcgccacgg
     3901 tcaaggcgcg ccccggcgca ctggaacgca ccgtattcga actgcaagcc aaagactggc
     3961 gcaccaaggc cgcgccggac ctgaacggtg cacaattggc cgaatggatg ggcgtgctca
     4021 aacgccaggg ggtcacgagt tttggctact acccggacaa cttcctggaa aattccccgg
     4081 acttgaaaac cgtgcgtccg gccctttcca accaatggaa cccttgacca tgttcgatag
     4141 aatcctggct ttattcg
//
LOCUS       DATCUN010000200         3914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_158909, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000200 DATCUN010000000
VERSION     DATCUN010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158909"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VIO83_08020"
     CDS             complement(<1..125)
                     /locus_tag="VIO83_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016395386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331254.1"
                     /translation="MSLRSLALVACCLLLVACNKVNQENFSRLKAGMSKAEVEQLL"
     gene            complement(296..1306)
                     /locus_tag="VIO83_08025"
     CDS             complement(296..1306)
                     /locus_tag="VIO83_08025"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEY8331255.1"
                     /translation="MSRVTLSRYLIEETRSNHTPADLRFLIEVVARACKEISHAVSKG
                     ALGGVLGSMGTENVQGEVQKKLDVLSNDILLEANEWGGHLAGMASEEMEQAYQIPGQY
                     PKGAYLLVFDPLDGSSNIDVNVSVGTIFSVLRCPDDCDSSCDLGEHAFLQPGTRQVAA
                     GYAIYGPQTMLVLTLGNGVKGFTLDRELGSFELTHDNMHVPESTAEFAINMSNQRHWE
                     APVKRYVDELLAGKEGPLGKNYNMRWIASMVADVHRILTRGGVFMYPRDGRDPSMPGK
                     LRLMYEANPMSMIVEQAGGAATTGTQRILDIQPTSLHQRVPVFLGSKEEVERISGYHQ
                     NA"
     gene            1490..1897
                     /locus_tag="VIO83_08030"
     CDS             1490..1897
                     /locus_tag="VIO83_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009396120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY8331256.1"
                     /translation="MTKHLLVGADPERLETWIKFPGNSLCRDCRATCCSLPVEVRLKD
                     LIRLGVIDEFEAGEPPKQIAKRLMKEGTVGHFNQKSGIFTLQHHSSGDCLYLDQKTRL
                     CTVYANRPDTCRNHPQIGPKPGYCAFQPKPGRR"
     gene            complement(1924..2859)
                     /locus_tag="VIO83_08035"
     CDS             complement(1924..2859)
                     /locus_tag="VIO83_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8331257.1"
                     /translation="MDILQAMRVFARVVDCGSFTAAAQLLELSTAQVSRLVSELEGSL
                     SARLLHRTTRRLRLTEVGERYLQRCRQILAEVDEATAEASGAHLTPSGRLRVHSFTGV
                     GIQYLVPLAARYAEQYPDVSLDLSLSQRTPDLLEEGHDVVITHSRELPDSDLIAQRLG
                     CMYSVVCAAPAYLQQYGTPQTPEELREHRCLRLLDPVYPGDWVFERLGAMQVINPGQS
                     FQVNVSEAMVEAAAAGMGVCLLPNFVAARALREGSLVRLLPAWQLHERNIYALYSSRR
                     FLDAKIKTWVEFIKAELPRILERDAKDLDNPAYWA"
     gene            complement(3238..3495)
                     /locus_tag="VIO83_08040"
     CDS             complement(3238..3495)
                     /locus_tag="VIO83_08040"
                     /inference="COORDINATES: protein motif:HMM:NF022429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2790 domain-containing protein"
                     /protein_id="HEY8331258.1"
                     /translation="MKTKALFLTAMLASASVFAADASAQSEKAREYAYGMNLDVAEVV
                     AIEEAPHALCEPVNSKMTYRDSKGQTHTVSYVKQSQDVGCY"
     gene            complement(3695..>3914)
                     /locus_tag="VIO83_08045"
     CDS             complement(3695..>3914)
                     /locus_tag="VIO83_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY8331259.1"
                     /translation="INPTKGKKLDNMRASGKDETIQLVPPIRFTLEQALEFIDDDELV
                     EVTPKSIRLRKKYLNENDRKRYERSSKL"
BASE COUNT          691 a         1257 c         1323 g          643 t
ORIGIN      
        1 agcagctgct cgacctcggc cttgctcatg ccggccttga gcctggagaa gttttcctgg
       61 ttgaccttgt tgcaggcgac cagcagcagg cagcaggcga ccagggcgag ggaacgcagc
      121 gacatcgggg gaactcctga aaatggaatg cggcaagcat ggcacaggcg ccgactgcca
      181 acgttgcgtt ggacggcaaa agcgccggcg cgttccccgc aaagcacgac gccggccaca
      241 agggccggcg tcaggggacg gagcgcgcgc tccgtccatg gccgtgaggc gaggatcagg
      301 cgttctggtg atagccgctg atgcgctcga cctcttcctt ggagccgagg aacaccggca
      361 cgcgctggtg cagcgaggtc ggctggatat cgaggatgcg ctgggtgccg gtggtggcgg
      421 cgccgccggc ctgctcgacg atcatcgaca tcgggttggc ctcgtacatc aggcgcagct
      481 tgccgggcat cgacggatcg cggccgtcgc gcgggtacat gaacacgccg ccgcgggtga
      541 ggatgcgatg cacgtcggcc accatcgagg cgatccagcg catgttgtag ttcttgccca
      601 gcgggccttc cttgccggcc agcagctcgt ccacgtagcg cttgaccggg gcttcccagt
      661 ggcgctggtt ggacatgttg atggcgaact cggcggtgct ttccggcacg tgcatgttgt
      721 cgtgggtcag ctcgaaggag cccagctcgc ggtcgagggt gaagcccttg acgccgttgc
      781 cgagggtcag caccagcatg gtctgcgggc cgtagatggc atagccggcg gccacctggc
      841 gggtacccgg ctggaggaag gcgtgctcgc cgaggtcgca gctgctgtcg cagtcgtccg
      901 ggcagcgcag cacggagaag atggtgccga ccgagacgtt gacgtcgatg ttggaagagc
      961 cgtccagcgg atcgaacacc agcaggtagg cgcccttcgg gtactggccg ggaatctggt
     1021 aggcctgctc catttcctcg gaagccatgc cggccaggtg gccgccccac tcgttggctt
     1081 ccagcaggat atcgttggac agcacgtcga gtttcttctg cacctcgccc tgcacgttct
     1141 cggtgcccat gctgcccagc acgccgccca gcgcgccctt ggacaccgca tggctgatct
     1201 ccttgcaggc gcgcgccacc acttcgatga ggaagcgcag atcggccggg gtgtggttgc
     1261 tgcgggtctc ttcgatcaga tagcgactga gggtaacgcg ggacatgggc ggctccggag
     1321 gggggcagga aaaaatctgc ggcattttaa cccgagttgc agcgcgccgc ctcctgccgc
     1381 gcatcaatag ctcggcattc ggccgaccgg ttgccggcaa aaactgctgc gcaacgggcg
     1441 ccgcttgctt atcatcgcgc gccttccgcc cttcaccctg cgaaccccga tgaccaaaca
     1501 cctgctggtc ggcgccgatc ccgagcgcct ggaaacctgg atcaagttcc ccggcaacag
     1561 cctgtgccgc gactgccgcg ccacctgctg cagcctgccg gtggaggtgc gcctcaagga
     1621 cctgatccgc ctcggcgtga tcgacgagtt cgaggccggc gagccgccca agcagatcgc
     1681 caagcggctg atgaaggaag gcaccgtcgg ccacttcaac cagaagtccg gcatcttcac
     1741 cctgcagcac cactccagcg gcgactgcct gtacctggac cagaagaccc gtctgtgcac
     1801 ggtctacgcc aaccgcccgg acacctgccg caaccatccg cagatcggcc cgaagcccgg
     1861 ctactgcgcc ttccagccca agcccggccg ccgctgaggc gccggcacgc cccgcgccga
     1921 agctcaggcc cagtaggccg gattgtcgag atccttggcg tcgcgctcga ggatgcgcgg
     1981 cagctcggcc ttgatgaact cgacccaggt cttgatcttg gcatcgagga agcgccgcga
     2041 cgaatacagc gcgtagatgt tgcgctcgtg cagctgccag gcaggcagca ggcgcaccag
     2101 gctgccctcg cgcagcgccc gggcggcgac gaagttgggc agcagacaga cgcccatgcc
     2161 ggccgcggca gcctccacca tcgcctcgga gacgttgacc tggaagctct gcccggggtt
     2221 gatcacctgc atcgcgccga ggcgctcgaa cacccagtcg cccggataca ccggatcgag
     2281 caggcgcagg cagcggtgct cgcgcagctc ttcgggcgtc tgcggcgtgc cgtactgctg
     2341 cagataggcc ggcgcggcgc acaccacgct gtacatgcag ccgagacgtt gggcgatcag
     2401 gtccgaatcg ggcagttcgc gggagtgggt gatcaccacg tcgtgccctt cctcgagcag
     2461 gtcgggcgta cgctgcgaca gggacagatc cagactgacg tccggatact gctcggcata
     2521 gcgcgccgcc aggggcacca ggtactggat gcccacgccg gtgaaggagt gcacgcgcag
     2581 gcgtccgctg ggggtgagat gggccccact ggcctcggca gtggcctcgt cgacctcggc
     2641 aaggatctgc cggcagcgct gcagatagcg ctcgcccacc tcggtcaggc gcaggcgccg
     2701 ggtggtgcga tgcagcaggc gggcgctcag gctgccttcc agctcggaca ccagccgcga
     2761 cacctgggca gtggacagct cgaggagctg ggccgcggcg gtgaagctgc cgcagtcgac
     2821 gacccgggcg aagacgcgca ttgcctgcag gatatccatc gcgaaccttg cctccagagc
     2881 ggcatgccat ccagccgcat accgaccaga aaaatccgcg ccaatatacg tcagcagcgg
     2941 ctcatgacca aaacggcagg cgtgcccggc ggcaagacag gccgacgacg gccacaggat
     3001 tgcagcattg ctgggcgaca agacgccacg cagccgcaca gggctgccat gcgttgccgc
     3061 agcagtcgct agagaagggg gaaccgcgga gtcgtgcggc ggaaggggcg gccctgacgg
     3121 tcgatcaggc ctgttgttgc gggtcgggcg cagcaggaag ggcgatgtcg tcgtgcgggc
     3181 gcatcaacaa cgtccgcagg cgctggccac cgaagcggta gcgcctgccg ggaggactca
     3241 gtagcagccg acgtcctggc tctgcttgac gtagctgacc gtgtgggtct gacccttgga
     3301 gtcgcgatag gtcatcttgc tgttgaccgg ttcgcacaga gcgtgcggcg cctcttcgat
     3361 ggccaccact tcggcgacat ccaggttcat gccgtaggca tattcgcggg ctttttcgct
     3421 ctgcgccgag gcatcggcgg cgaacacact ggccgaagcc agcatggcag tcaggaacag
     3481 ggctttggtt ttcatggcaa ctcgatctcc tgcgtggaaa ggaattaccc gatggaggga
     3541 tcggcttcgg gcgtgccgtt attgtcaggc agcccgggaa acggaaaaac agcggcgcga
     3601 acaagccagt attacgcagg acgcaacaat cacacgaaaa aagcccccgc cggcaggacc
     3661 ggcgggggct tctggtttca gctcagcgca agacttacag cttgctgctg cgctcgtaac
     3721 gcttgcggtc gttctcgttc aggtacttct tgcgcagacg gatcgacttc ggggtcactt
     3781 cgaccagttc gtcgtcgtcg atgaactcca gcgcctgttc cagggtgaag cggatcggcg
     3841 gaaccagctg gatggtctcg tccttgccgg aagcacgcat gttgtcgagc ttcttgccct
     3901 tggtggggtt gatc
//
LOCUS       DATCUN010000201         1959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_121208, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000201 DATCUN010000000
VERSION     DATCUN010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121208"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VIO83_08050"
     CDS             complement(<1..313)
                     /locus_tag="VIO83_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008018621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HEY8331260.1"
                     /translation="MPAIVRPGLFLASLLVSVFSSLSGCIDSGGIAPQAELADGAELP
                     TDAAIRHAAQSADWPRAQWWQAYGDAQLNAWVERALAGSPGLAMAAAWRMAASVGNSA
                     PS"
     gene            561..1508
                     /locus_tag="VIO83_08055"
     CDS             561..1508
                     /locus_tag="VIO83_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8331261.1"
                     /translation="MDILFNMRAFVCVAETGSFTAAAARLDLTTSYVSRAVATLETHL
                     RTRLLHRTTRRIALTEAGQRYLMRCEQILGYIEEAEAEASDAHARPAGTLKVHAMTGI
                     GQHYLIDAAAEYNAKYPDVSFDLTLANRTTDILDEGYDISVVIARELPDSGFISKQLG
                     KTYSVLCAAPSYIDKHGFARQPADLAKHRCLRLVNSVMSLDKWLLDGPDGEELVTVSQ
                     TPFQVNTADAMSVALKAGMGIGALPIYAALQSLQDGSLMRVLPNHTLFPLGVFALYPS
                     RQYLDAKTRTWVEFLRDYMPQRLAADESHLLACSTQLMG"
     gene            complement(1519..>1959)
                     /locus_tag="VIO83_08060"
     CDS             complement(1519..>1959)
                     /locus_tag="VIO83_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8331262.1"
                     /translation="AAGLSLLLALLVQISRLAQPADAPRPGQGRRAILLEQFRELVNR
                     QFRQHWPLELYAERLGISAAQLGRICREELGESAMGLINARLMREAQRQLAYTELEIK
                     QIAHALGFRDTSYFSRYFRKQAGIKPSEFRAAFQSVAAAPAAGE"
BASE COUNT          346 a          710 c          632 g          271 t
ORIGIN      
        1 ccgacggcgc cgaattgccc accgacgcgg ccattcgcca cgccgcagcc atggccaggc
       61 cgggactgcc ggccagtgca cgctcgaccc aggcgttcag ctgcgcatcg ccataggcct
      121 gccaccactg cgcgcgcggc cagtcggcgc tctgggcggc gtggcgaatg gccgcgtcgg
      181 tgggcaattc ggcgccgtcg gccagctcgg cctgcggggc tatgccaccg ctgtcgatgc
      241 agccggaaag ggaagaaaat acggaaacaa gcagactggc gaggaacaga ccgggccgaa
      301 cgatggcggg cactgcagca catcctggat gggggcgtgg ggtcggggaa ggctggcgat
      361 tctaagagcg cccgcgggcg ataataagca cgcagataga tgatggattg tgtacggaac
      421 ggtcataatc attggcaagc ttcgtgacag aattcacgcc agccgacagc ctgcgccaca
      481 aggcctgcag ccgcggcctt cctgccaacg gtcaaggatt tccagcgcag ccaaaccgcc
      541 ctcccgcccg gaagcgatca atggatatcc tcttcaacat gcgcgccttc gtctgcgtgg
      601 cggaaaccgg cagtttcacc gctgccgcgg cgcgcctcga cctgaccacc tcctacgtct
      661 cgcgcgccgt cgccaccctg gaaacccacc tgcgcacgcg cctgctgcac cgcacgacgc
      721 gacgcatcgc cctcaccgag gccggccagc gctatctgat gcgctgcgag cagatcctcg
      781 gctacatcga ggaagccgag gccgaggcca gcgacgcgca cgcgcgtccg gccggcaccc
      841 tcaaggtcca cgcgatgacc ggcatcggcc agcactacct gatcgacgcc gccgccgaat
      901 acaacgcgaa atacccggac gtcagcttcg acctgaccct ggccaaccgc accaccgaca
      961 tcctcgacga gggctacgac atctcggtgg tgatcgcccg cgagctgccg gactccggct
     1021 tcatctccaa gcagctgggc aagacctaca gcgtgctgtg cgccgcgccg agctacatcg
     1081 acaagcacgg cttcgcccgc cagcccgccg acctggccaa gcaccgctgc ctgcgcctgg
     1141 tcaactcggt gatgagcctg gacaagtggc tgctcgacgg ccccgacggc gaggaactgg
     1201 tgaccgtcag ccagaccccc ttccaggtca acaccgccga tgccatgagc gtggcgctga
     1261 aggccggcat gggcatcggc gccctgccga tctacgcggc gctgcagagc ctgcaggacg
     1321 gcagcctgat gcgcgtgctg cccaaccaca cgctgttccc gctcggcgtg ttcgccttgt
     1381 acccgtcgcg ccagtacctg gacgccaaga cccgcacctg ggtcgagttc ctgcgcgact
     1441 acatgcccca gcgcctcgcc gccgacgaaa gccacctgct cgcctgcagc acgcaactga
     1501 tgggctagcg accggcgctc actcgccggc cgcgggcgcc gcggccacgg actggaacgc
     1561 ggcgcgaaat tcgctcggct tgatccccgc ctgcttgcgg aagtagcggc tgaagtagga
     1621 ggtgtcgcga aagccgaggg cgtgggcgat ctgcttgatc tccagttccg tgtaggccag
     1681 ctggcgctgc gcctcgcgca tcagccgcgc attgatcagg cccatcgccg actcgcccag
     1741 ctcctcgcgg cagatgcggc cgagctgcgc cgcgctgatg ccgaggcgct cggcgtacag
     1801 ctccagcggc cagtgctggc ggaactggcg gttgaccagc tcgcggaact gctcgaggag
     1861 aatcgcccgc cggccctggc ccggccgcgg ggcatcggcc ggctgcgcca ggcgggagat
     1921 ctgcaccagc agggcgagca gcagcgacag cccggcggc
//
LOCUS       DATCUN010000202         4907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_253549, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000202 DATCUN010000000
VERSION     DATCUN010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253549"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VIO83_08065"
     CDS             complement(<1..594)
                     /locus_tag="VIO83_08065"
                     /EC_number="1.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate
                     dehydrogenase"
                     /protein_id="HEY8331263.1"
                     /translation="MHTQQVQQRFAHKVAVITGAAQGIGRRVAERMAAEGGRLVLVDR
                     SELVHELADELAGVAEVLTLTADLEQAGDCQRVMAAAVERFGRLDILVNNVGGTIWAK
                     PFEHYRENEIEAEVRRSLFPTLWCCHAALPQMLTQGSGAIVNVSSIATRGVNRAPYGA
                     AKGGVNALTACLALETAERGIRVNATAPGGTEAPPRRI"
     gene            complement(659..1669)
                     /gene="benC"
                     /locus_tag="VIO83_08070"
     CDS             complement(659..1669)
                     /gene="benC"
                     /locus_tag="VIO83_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020192424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase electron transfer
                     component BenC"
                     /protein_id="HEY8331264.1"
                     /translation="MSHKIALNFEDGVTRFIDAKGSETVADAAYRQGINIPLDCRVGA
                     CGACKCQVESGSYELGEDFIEDALSADEAAQGMVLTCQMRAQSDCVVRVPVASSACRI
                     KQGSFRAAIREVQALSDSTIRLAIEGDELGKLAFLPGQYVNLQVPGSEQTRAYSFSSM
                     PQDGKVSFLIRNVPGGLMSGYLTQLAKVGDQMTLTGPLGSFYLRDVRRPLLLLAGGTG
                     LAPFLAMLEKLAASGSDQPVHLIYGVTNDFDLVEMDKLAEFAQRLPNFTYSACVANPD
                     SQHPLKGYVTQHIAPGQLNDGDVDIYLCGPPPMVEAVNQDLRQRGIQPANFYYEKFAA
                     SA"
     gene            complement(1790..2278)
                     /gene="benB"
                     /locus_tag="VIO83_08075"
     CDS             complement(1790..2278)
                     /gene="benB"
                     /locus_tag="VIO83_08075"
                     /EC_number="1.14.12.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007944753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase small subunit"
                     /protein_id="HEY8331265.1"
                     /translation="MSISYEAVRDFLYQEARFLDDKDWDSWLELYAEDATFWMPAWDD
                     NDELTEDPQKEISLIWYGRRAGLEDRVFRIRTERSSASIPDTRTSHNISNLEIVEQGE
                     GFCKVRFNWHTLSFRYKTVDQYYGTSFYTLDTRGANPLVKAKKVVLKNDYVRHVIDVY
                     HI"
     gene            complement(2275..3639)
                     /gene="benA"
                     /locus_tag="VIO83_08080"
     CDS             complement(2275..3639)
                     /gene="benA"
                     /locus_tag="VIO83_08080"
                     /EC_number="1.14.12.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015270384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase large subunit"
                     /protein_id="HEY8331266.1"
                     /translation="MSVGREYLNSLLEENQEKGLYRCKREMFTDAELFELEMKHIFEG
                     NWIYMAHESQIPEKNDYYTVTMGRQPVFIARNRAGELNAFINSCSHRGATLCRFKHGN
                     KATYTCPFHGWTFDTNGKLLKVKDPSEAGYPDSFNCSGSHDLTKIARFESYRGFLFGS
                     LNADVVPLVEHLGESAKIIDMIVDQSPEGLEVLRGSSTYVFEGNWKLQAENGADGYHV
                     TATHWNYAATQGNRKDKDAGDDIRAMSAGGWAKKGGGFYSFENGHLLLWTRWDNPQDR
                     PLYERRDQLAQEVGEARADWMIGNSRNLCLYPNLYLMDQFSSQLRIARPISVDKTEVT
                     IYCIAPKGESAEARAHRIRQYEDFFNVSGMATPDDLEEFRACQEGFQGRALPWNDMSR
                     GAKHWIEGADEAAKEIQLQPLMSGVRAEDEGLFVIQHRHWLATMQKAIEQEQQNLIHV
                     EAGK"
     gene            complement(3858..4811)
                     /locus_tag="VIO83_08085"
     CDS             complement(3858..4811)
                     /locus_tag="VIO83_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8331267.1"
                     /translation="MDRRLLNEKSLVFANADPHAVSDYVNQHVGTHRILLPNCGHPEA
                     SLNHRAFGSLDLCQISYGSRVQVTSPALETLYHLQILLRGHCLWLGRGEEHYFTPGEL
                     LLINPDDPVDLTYSDDCEKFIVKLPATFLDKACSENQWRHPGEGVRFTPRHHLEQLEG
                     FVSLLSLVCQEAEAEQAMPLVQEHYAKIIASKLLGLPGCNVSREPLTAGCPSFERLVE
                     FIERNLKQDISLEQLAELAHMSPRSLYALFERHAGTTPKHYIRQRKLETIHARLSDPQ
                     AAVRNVTEVALDYGFLHLGRFSECYKSTFGELPSDTLRRRN"
BASE COUNT          765 a         1484 c         1701 g          957 t
ORIGIN      
        1 gatgcgccgc ggcggcgcct cggtgccgcc cggtgcggtg gcgttgacgc ggatgccgcg
       61 ctcggcggtt tccagcgcca ggcaggcggt cagcgcgttg acgccgccct tggccgcgcc
      121 gtagggcgcg cggttgacgc cgcgggtagc gatggaggac acgttgacga tggcgccgct
      181 gccctgcgtg agcatctgcg gcagcgccgc gtggcagcac cacagggtgg ggaacagcga
      241 gcggcgcacc tcggcctcga tctcgttttc ccggtagtgc tcgaacggct tggcccagat
      301 ggtgccgcca acgttgttga ccaggatgtc gagacggccg aagcgttcga cggcggccgc
      361 catcacccgc tggcagtcgc cggcctgttc gaggtcggcg gtgagggtca gcacctcggc
      421 gacgccggcc aactcgtcgg ccagttcatg gaccagctcg gagcggtcga ccagcaccag
      481 gcgaccgccc tcggcggcca tccgctcggc gacgcgacgg ccgataccct gggcggcgcc
      541 ggtgatcacc gcgaccttgt gtgcaaagcg ttgttgtact tgttgtgtgt gcatggcgag
      601 gcatccgtga aaaaaatagc ccgcgcggct tcagccggac cgcgggccca aaggactgtc
      661 aggcactggc cgcgaacttc tcgtagtaga agttggccgg ctggatgccg cgctggcgca
      721 gatcctggtt gactgcttcg accatcggcg gcggaccgca caggtagatg tcgacgtcgc
      781 cgtcgttcag ctggcccggc gcgatgtgct gggtcacgta acccttgagc gggtgctggc
      841 tgtccgggtt ggccacgcag gcgctgtagg tgaagttggg caggcgctgg gcgaattccg
      901 ccagcttgtc catctccacc aggtcgaagt cgttggtgac gccgtagatc aggtgcaccg
      961 gctggtcgct gccgctggcg gcaagctttt ccagcatggc caggaacggc gccagcccgg
     1021 taccgccagc cagcagcagc agcggccggc gcacgtcgcg caggtagaag ctgcccagcg
     1081 ggccggtcag ggtcatctgg tcgccgactt tcgccagctg ggtgaggtag ccgctcatca
     1141 gcccgcccgg cacgttgcgg atcaggaacg acaccttgcc gtcctggggc atcgagctga
     1201 acgagtaggc gcgggtctgc tcgctgcccg gcacctgcag gttgacgtac tggccgggca
     1261 ggaacgccag cttgcccagt tcgtcgccct cgatggccaa gcggatggtg ctgtcggaaa
     1321 gcgcctgcac ttcacggatg gcggcgcgga agctgccctg cttgatgcgg caggcgctgg
     1381 aggcgacggg cacgcgcacc acgcagtcgc tctgcgcgcg catctggcag gtcagcacca
     1441 tgccctgggc ggcctcgtcg gcactgaggg cgtcctcgat gaagtcttca cccagttcat
     1501 agctgccgga ttcgacctgg cacttgcagg cgccgcaggc gccgacccgg cagtccagcg
     1561 gaatattgat gccctggcgg tacgcggcgt cggccacggt ttcgctgcct ttggcgtcga
     1621 tgaaacgggt aaccccgtct tcgaaattca gtgcgatttt gtgcgacatg ctgcacctcg
     1681 cttggagctg gtatttcgcg ccggccgcag ccgtgcgcgc acgcagggtg gaaggcgaac
     1741 cttccttccg gagcgatgcg ggccgctggc ggcggcccgc tcgcttcttt tagatgtggt
     1801 agacgtcgat cacgtggcgc acgtagtcgt tcttcagcac caccttcttc gccttgacca
     1861 gcggattggc gccgcgggta tcgagggtgt agaagctggt gccgtagtac tggtccacgg
     1921 tcttgtagcg gaagctcagg gtgtgccagt tgaagcgcac cttgcagaag ccttcaccct
     1981 gctcgacgat ttccaggttg ctgatgttgt gcgaggtacg ggtgtccgga atgctcgcgc
     2041 tggatcgctc ggtgcggatg cggaagacgc ggtcttccaa gccggcgcgg cggccgtacc
     2101 agatcagcga gatttccttc tgcggatcct cggtcagctc gtcgttgtcg tcccaggccg
     2161 gcatccagaa ggtggcgtcc tcggcgtaca gctccagcca gctgtcccag tccttgtcgt
     2221 cgaggaagcg ggcttcctga tagaggaaat cgcgtacagc ttcgtaggag atgctcattt
     2281 gccggcctcc acgtggatca ggttctgttg ttcttgttcg atggccttct gcatggtcgc
     2341 cagccagtga cggtgctgga tgacgaacag accttcgtct tcggcgcgca caccgctcat
     2401 cagcggctgc agctggattt ccttggcggc ctcgtcggcc ccctcgatcc agtgcttggc
     2461 accacgggac atgtcgttcc acggcagggc gcgaccctgg aagccttcct ggcaggcgcg
     2521 gaattcctcg aggtcgtccg gagtggccat gccgctgacg ttgaagaagt cttcgtactg
     2581 gcggatgcgg tgggcgcggg cctcggcgct ctcgcctttc ggcgcgatgc agtagatggt
     2641 gacttcggtc ttgtcgacgg agatcggccg ggcgatgcgc agctgcgagc tgaactggtc
     2701 catcaggtac aggttcgggt acaggcacag gttgcgcgag ttgccgatca tccagtcggc
     2761 gcgagcctca ccgacttcct gagccagctg gtcacggcgc tcgtacagcg gacggtcctg
     2821 cgggttgtcc cagcgggtcc acagcagcag gtggccgttc tcgaaggagt agaaaccgcc
     2881 gcccttcttg gcccagccgc cggcgctcat ggcgcggatg tcgtcgccgg catccttgtc
     2941 cttgcggttg ccctgggtgg ccgcgtagtt ccagtgggtc gcggtgacgt ggtagccgtc
     3001 ggcaccgttc tcggcctgca gcttccagtt gccctcgaac acgtaggtgc ttgatccgcg
     3061 cagcacttcc aggccctcgg gggactggtc gacgatcatg tcgatgatct tcgccgactc
     3121 gccgaggtgc tcgaccagcg ggaccacgtc ggcattcagg ctgccgaaca ggaagccgcg
     3181 ataggactcg aagcgggcga tcttggtcag gtcatgggaa ccgctgcagt tgaagctgtc
     3241 cgggtagccg gcttcgctcg gatccttgac cttgagcagc ttgccgttgg tgtcgaaggt
     3301 ccagccgtgg aacgggcagg tgtaggtcgc cttgttgccg tgcttgaagc ggcacagggt
     3361 ggcgccacgg tggctgcagg agttgatgaa ggcgttcagt tcgccggcgc ggttgcgggc
     3421 gatgaacacg ggctgacggc ccatggtcac ggtgtagtag tcgttcttct cgggaatctg
     3481 gctctcgtga gccatataga tccagttgcc ttcgaagatg tgtttcatct ccagctcgaa
     3541 cagctcggca tcggtgaaca tctcccgctt gcagcggtag aggccctttt cctggttttc
     3601 ttcgagcagg gagttcaggt attcgcggcc cacagacatg tctgcgttct cctttgttgt
     3661 ttttgtactg tggagttcag actaagcctg cgaccggacg ggcaatatcc gctttctgca
     3721 cgatcgctat ccgctttttg caggatgtgg aaaacgccgg gcggaaacgg acaaacccgc
     3781 gcgacggcgg gtgagcagga ggcaggcgga ggaggtgcag aggcacggcg ggccgacgcc
     3841 cgcccggtgg cgcagggtca gttgcggcgg cgcagggtgt ccgagggcag ttcgccgaag
     3901 gtgctcttgt agcactcgga gaagcggccg aggtgcagga aaccgtagtc cagggcgact
     3961 tcggtgacgt tgcgcaccgc cgcctgcggg tcgctcaggc gggcatggat cgtctccagc
     4021 ttgcgctggc ggatgtagtg cttgggcgtg gtgccggcat gccgctcgaa cagcgcatac
     4081 agcgagcgcg gactcatgtg cgccagctcg gccagctgtt cgaggctgat gtcctgcttg
     4141 aggttgcgct cgatgaactc gaccagacgc tcgaacgacg ggcagccggc cgtgagcggc
     4201 tcgcggctga cgttgcagcc cggcaggccg agcagcttgc tggcgatgat cttggcgtag
     4261 tgctcctgca ccaggggcat cgcctgttcg gcctcggctt cctggcagac caggctcagc
     4321 aggctgacga agccttccag ctgctcgagg tggtggcgcg gcgtgaagcg cacgccctcg
     4381 ccggggtgac gccactggtt ctcgctgcag gccttgtcga gaaaggtggc cggcagcttg
     4441 acgatgaact tctcgcagtc gtccgagtag gtcaggtcga ccggatcgtc ggggttgatc
     4501 agcagcagtt cgccgggtgt gaagtagtgc tcctcgccgc gaccgagcca caggcagtgg
     4561 ccgcgcagga ggatctgcag gtgatagagg gtttccaggg ctggcgaggt gacctgcacg
     4621 cggctgccgt agctgatctg gcaaagatcc aggctgccga aggcacgatg attcaggctc
     4681 gcctccggat gcccgcagtt gggcaggagg atccgatggg tgccgacatg ctggttgacg
     4741 tagtccgata ccgcatgcgg atcggcattg gcgaaaacca gactcttttc gttcaacaaa
     4801 cgacggtcca tgactcgcgc agctcttgta gttgttattg gcacaccggg gtggctgagg
     4861 acacagcgcc accctatttt ctgcattgac ccacaacact gcgtccc
//
LOCUS       DATCUN010000203         7093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_234009, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000203 DATCUN010000000
VERSION     DATCUN010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234009"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VIO83_08090"
     CDS             <1..194
                     /locus_tag="VIO83_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331268.1"
                     /translation="RLRRIRQPYPALRLFAWLVLLAGAGSASGVLLVPALLLGLAASA
                     ALAWVFFTAAGELGAEPAA"
     gene            complement(219..779)
                     /locus_tag="VIO83_08095"
     CDS             complement(219..779)
                     /locus_tag="VIO83_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY8331269.1"
                     /translation="MADDDFSQFLSEMRGVKRIKVEERADTGKPKADRQQVANRQQNA
                     LLSQQTTTVDGLSDLFVIDVGAEDELYWVRDGVQEGQMRKLKAGQIPFEGSLDLHGMN
                     VETARATLWEFLAEAARFEVRCVRVTHGKAARTDGRRPMVKSHVNTWLRQHPQVLGFT
                     SCVARHGGTGAVYVLLKRTMLEGRDE"
     gene            875..1795
                     /gene="prmB"
                     /locus_tag="VIO83_08100"
     CDS             875..1795
                     /gene="prmB"
                     /locus_tag="VIO83_08100"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY8331270.1"
                     /translation="MSANHSRLQSIRDLIRWGVSRFHGAELFFGHGTDNAWDEARQLV
                     LGGLHLPWDTAESWLDCRLEADELEAVCALLQRRIDERIPAAYLLGEAWFCGQPYCVD
                     ERVLVPRSPIAELIDRHFAPWLKGTPTRILDLCTGSGCIGIACAHAFPAAEVVLADLS
                     FDALEVANLNIERHELEERVYTVQGDGFDGLPGQRFDLIVSNPPYVDAEDFADMPAEF
                     HHEPAMGLACGDDGLDLVRRMLAEAADHLNDDGVLVIEVGNSQVHVESAYPEVEFTWV
                     EFANGGHGVFVLSAEQCRRHQALFRARLAS"
     gene            complement(1851..2819)
                     /locus_tag="VIO83_08105"
     CDS             complement(1851..2819)
                     /locus_tag="VIO83_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8331271.1"
                     /translation="MPSTRLLRTALLLTLLLCCALVRAAPPSILQWPVELVVPGGILH
                     GSLMLPETAQPAPVVLMIAGSGPTDRDGNNPAGGNNDSLRHLALGLAERGIASLRYDK
                     RGVAASHAAAPHEEQLSVEAYVADAVAWGQLLKADPRFSQLILLGHSEGALIATLAAA
                     DSGADALVSLAGIAHPLGTVLREQLQGRLPPTLAAETETILRELERGRVVPQVSPKLR
                     VLLRPSVQPYLITLLRQRPAEAFAQVPIPALIVQGTRDFQVDPQEALLLQQAKPEAQL
                     LLVEGMNHVLRVVPADAPPFASYNDPQLPLAGELLDGLARFILPAP"
     gene            2941..4032
                     /gene="aroC"
                     /locus_tag="VIO83_08110"
     CDS             2941..4032
                     /gene="aroC"
                     /locus_tag="VIO83_08110"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY8331272.1"
                     /translation="MSGNTYGKLFTVTTAGESHGPALVAIVDGCPPGLDISLEDLQRD
                     LDRRKPGTSRHTTQRQEADEVEILSGVFEGKTTGCAIGLLIRNTDQKSKDYSAIKDLF
                     RPAHADYTYHHKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLATLGISVRGYMSQLG
                     PIEIPFKTWGSVEQNAFFSPDPDKVAELEAYMDQLRRDQDSVGAKITVVAEGVPPGLG
                     EPIFDRLDAELAHALMSINAVKGVEIGAGFASVAQRGTEHRDELTPAGFLSNNAGGIL
                     GGISSGQPIVAHLALKPTSSITTPGRSIDIHGNPVEVITKGRHDPCVGIRATPIAEAM
                     MAIVLMDHLLRHRAQNADVQVSTPVLPQL"
     gene            4088..5242
                     /locus_tag="VIO83_08115"
     CDS             4088..5242
                     /locus_tag="VIO83_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8331273.1"
                     /translation="MTSALPYWRLSGFYFSYFALLGATAPFLALYFHHLGFSAARIGE
                     LVAIPMLMRCVAPNLWGWLGDVTGRRLLIVRLGALCTLVSFASIFYRQDYAWLALVMA
                     LHAFFWHAVLPQFEVITLAHLREQAARYSQIRLWGSIGFICTVVGLGALFERLSLDAY
                     PWALIGIMLAIVLSSLWIPNAQPQVHASEAARGGFLQQLRRPGVPAFYAAVALMLLSH
                     GPYYTFITLHLESLGYSRTQIGWLWALGVLAEILLFLVMARLLARFSVRQVLLASLLL
                     AALRWLLLGNLADHWPVLLLAQVMHAATFGSFHAAAIHFVRHSFAARQQGQGQALYAA
                     LSGSGAAVGALYAGYSWNSLGPAWTFAIASLAALLAAGIVVWRLREPAAT"
     gene            5281..5901
                     /locus_tag="VIO83_08120"
     CDS             5281..5901
                     /locus_tag="VIO83_08120"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016781482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HEY8331274.1"
                     /translation="MTDKRALLTRQIIEAGRFLYGRGWSPATSSNYSARLAADQALLT
                     VSGKHKGQLTDNDVLATDLAGNSLEPGKKPSAETLLHTQLYAWRADIGAVLHTHSVNA
                     TVLSRLTPGDYLLLEDYELQKAFSGVTTHEGRVVVPIFANDQDIARLAGQVQQWLEGH
                     ADCAGYLIRGHGLYTWGAQMSDALRQIEAFEFLFECELKTRQVLGR"
     gene            5945..6490
                     /locus_tag="VIO83_08125"
     CDS             5945..6490
                     /locus_tag="VIO83_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone dioxygenase"
                     /protein_id="HEY8331275.1"
                     /translation="MSRLSVYHESTPQVPNKVLTHAEDIAATLAEVGVRFERWQASAP
                     IAPGASQDEVIAAYRHEIDKLMSEQGYVTVDVISLDRSHPQKAELRAKFLDEHTHGED
                     EVRFFVAGRGLFTLHVGDQVYAVLCEKNDLISVPAGTPHWFDMGEEPHFVAIRLFNNP
                     EGWVAQFTGDSIAKHFPPLED"
     gene            6493..>7093
                     /gene="mtnC"
                     /locus_tag="VIO83_08130"
     CDS             6493..>7093
                     /gene="mtnC"
                     /locus_tag="VIO83_08130"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HEY8331276.1"
                     /translation="MPIKAILTDIEGTTSAVSFVFDVLFPYAREHLPAFVRDHAAEPA
                     VAAQLDAVRAESGEAEADVERVVAILLEWIAADRKATPLKALQGMVWEQGYRAGQLKG
                     HVYPDAVEALRQWHAAGYELYVYSSGSIQAQKLIFGCSEAGDLTPLFSGYFDTTSGGK
                     REAESYARIAAAIGRPAGEILFLSDVVQELDAARAAGMAT"
BASE COUNT         1028 a         2527 c         2400 g         1138 t
ORIGIN      
        1 tgcgcctgcg gcgcatccgc cagccgtacc cggcgctgcg cctgttcgcc tggctggtgc
       61 tgctggccgg cgccggctcg gccagcgggg tgctgctggt gccggcgctg ttgctgggcc
      121 tggccgccag tgcggcactg gcctgggtgt tcttcaccgc agccggggag ctgggggcgg
      181 agccggcggc ctgagtgggc cgtactgaac ggtccaggct actcgtcgcg cccctcgagc
      241 atggtccgct tcaacagcac gtagacggcg ccggtgccgc catgcctggc cacgcaggag
      301 gtgaagccga gcacttgcgg gtgctggcgc agccaggtgt tgacgtggct cttgaccatt
      361 ggccggcgac cgtcggtgcg tgccgcctta ccgtgggtca cgcgcacgca gcgcacctcg
      421 aagcgcgccg cctcggcgag aaactcccag agggtggcgc gcgccgtctc cacgttcatg
      481 ccgtgcaggt cgaggctgcc ctcgaagggg atctgcccgg ccttgagctt gcgcatctgg
      541 ccttcctgca cgccgtcgcg cacccagtac agctcgtcct cggcgccgac gtcgatgacg
      601 aacaggtcgg acagcccgtc gaccgtggtg gtctgctggc taagcagcgc gttctgctgg
      661 cggttggcga cttgctggcg atcggccttg ggtttgccgg tgtcggcgcg ctcctccacc
      721 ttgatgcgct tgactccgcg catctcgctg agaaattggg aaaaatcgtc gtctgccata
      781 cgggctctcc acgtcaaggc gggcatttta acggatgcgg cggcgcggcg ggcgttcggc
      841 tagaatgcgc gccctatcac cgaggagccg tgccatgagc gccaaccaca gccgtctgca
      901 aagcatccgt gacctgatcc gctggggtgt cagccgcttc catggcgccg agctgttctt
      961 cggtcacggt accgacaatg cctgggacga ggcgcgccag ctggtgctcg gcggcctgca
     1021 cctgccctgg gataccgcgg aaagctggct cgactgtcgt ctggaggcgg acgagctgga
     1081 ggcggtctgc gcgctgctgc agcggcgcat cgacgagcgc attccggcgg cctacctgct
     1141 cggcgaggcc tggttctgcg gccagccgta ttgcgtcgac gagcgcgtgc tggtgccgcg
     1201 ctcgccgatc gccgagctga tcgacaggca cttcgcgccc tggctcaagg gtacgcctac
     1261 gcgcatcctc gacctgtgca ccggctccgg ctgcatcggc atcgcctgcg cccacgcctt
     1321 cccggcggcc gaggtggtgc tggccgacct gtcgttcgac gcgctggagg tggccaacct
     1381 caacatcgag cgccacgaac tggaggagcg cgtctacacc gtgcagggcg acggcttcga
     1441 cggcctgccg ggccaacgct tcgacctgat cgtgtccaat ccgccctatg tcgacgccga
     1501 ggacttcgcc gacatgcccg ccgagttcca ccacgagccg gccatgggcc tggcctgcgg
     1561 cgacgacggt ctggacctgg tgcggcgcat gctcgccgag gccgccgacc acctgaacga
     1621 cgacggtgtg ctggtgatcg aggtgggcaa cagccaggtg cacgtcgagt ccgcctatcc
     1681 ggaagtggaa ttcacctggg tcgaatttgc caacggcggc cacggcgtgt tcgtgctcag
     1741 cgccgagcag tgccgtcggc accaggcgct gttccgcgcg cgcctggcgt cttgagcgtg
     1801 gggtgcgccc tgcgcaccat gagcatgccc tgcggtgcgc agggcgcacc ctacggggcg
     1861 ggcaggatga agcgcgccag gccatcgagc agttcgccgg ccagcggcag ctgcgggtcg
     1921 ttgtaggagg caaacggcgg cgcgtcggcc ggcacgacgc gcagcacgtg gttcatgccc
     1981 tcgaccagca gcagctgcgc ctccggcttg gcctgctgca acagcagcgc ctcctgcggg
     2041 tcgacctgga agtcgcgggt gccctggacg atcagcgccg ggatcggcac ctgggcgaag
     2101 gcttcggccg ggcgctggcg cagcagggtg atcaggtagg gctgcacgct cggccgcagc
     2161 agtacccgca gcttcggcga gacctgcggc accacgcggc cgcgttccag ttcgcgcagg
     2221 atcgtctcgg tttctgccgc cagcgtcggc ggcaggcgcc cctgcagttg ctcgcgcagc
     2281 accgtgccga gcggatgggc gatgccggcg aggctgacca aggcatcggc gccggagtcg
     2341 gcggcggcca gggtggcgat cagcgcaccc tcgctgtggc cgagcaggat cagctgggaa
     2401 aagcgcgggt cggccttgag cagctggccc caggccaccg catcggcaac gtaggcctcg
     2461 acgctcagct gttcctcgtg cggtgccgcc gcgtggctgg cggccacgcc gcgcttgtcg
     2521 tagcgcaggc tggcgatgcc gcgctcggcc aggcccaggg ccaggtgccg gaggctgtcg
     2581 ttgttgcccc cggcaggatt gttgccgtcg cggtcggtcg gtccggaacc ggcgatcatc
     2641 agcaccaccg gcgccggctg cgccgtttcc ggcagcatca ggctgccgtg caggatgccg
     2701 ccgggtacca ccagctccac cggccattgc aggatgctcg gcggcgcggc ccgcaccagg
     2761 gcgcaacaca gcagcagcgt cagcagcagg gcggtgcgta gcaggcgcgt gctgggcatg
     2821 ggggagggct ccgggcaagg tcggttggac tggcaggtgc gcgcagccgg cgtttcccca
     2881 gcggcggggc gcttgggtat actgccggcc ttcatttttt cccgccgcgg agcatcctgc
     2941 atgtctggca atacttacgg caagctgttc accgtcacca ccgccggtga aagccatggc
     3001 ccggcgctgg tcgccatcgt cgacggctgc ccgcccgggc tggacatctc gctggaagac
     3061 ctgcagcgcg acctcgaccg ccgcaagccc ggcaccagcc gccacaccac ccagcgccag
     3121 gaagccgacg aggtggaaat cctctccggc gtgttcgagg gcaagaccac cggctgcgcc
     3181 atcggcctgc tgatccgcaa caccgaccag aagtccaagg actactcggc gatcaaggat
     3241 ctgttccgcc cggcccacgc cgactacacc taccaccaca agtatggcct gcgcgactac
     3301 cgtggcggcg gccgcagctc ggcgcgcgag accgccatgc gcgtcgccgc cggcgccatc
     3361 gccaagaagt acctggcgac tctgggcatc agtgtacgcg gctacatgag ccagctcggt
     3421 ccgatcgaga tcccgttcaa gacctggggc agcgtcgagc agaacgcctt cttcagcccc
     3481 gatccggaca aggtggccga gctggaggcc tacatggacc agctgcgccg cgaccaggac
     3541 tcggtcggcg cgaagatcac cgtggtcgcc gaaggcgtgc cgccgggcct cggcgagccg
     3601 atcttcgacc gcctcgacgc cgaactggcc catgcgctga tgagcatcaa cgcggtcaag
     3661 ggcgtggaga tcggcgccgg cttcgccagc gtcgcccagc gcggcaccga gcaccgcgac
     3721 gagctgaccc cggcgggctt cctgtccaac aacgccggcg gcatcctcgg cggcatttcc
     3781 tccggccagc cgatcgtcgc ccacctggcg ctcaagccga cctcgagcat caccactccg
     3841 ggccgctcca tcgacatcca cggcaacccg gtggaagtga tcaccaaggg gcgccacgat
     3901 ccctgcgtcg gcatccgcgc cacgccgatc gccgaggcga tgatggccat cgtgctgatg
     3961 gaccacctgc tgcgccaccg cgcgcagaac gccgacgtgc aggtgagcac cccggtgctg
     4021 ccgcagctgt gagtgccggt gctggccgga tgccttcccg ggtagtccgg ccggcgctcg
     4081 ctagatcatg acctccgcat tgccctactg gcgcctgtcc ggcttctact tcagctactt
     4141 cgccctgctc ggtgcgacgg cgccgttcct cgccctgtat ttccatcacc tgggcttttc
     4201 cgcggcgcgc atcggcgagc tggtggccat ccccatgctg atgcgctgcg tggcgcccaa
     4261 cctgtggggc tggctgggcg atgtcactgg ccggcgcctg ctgatcgtgc gcctcggtgc
     4321 gctctgtacc ctggtcagtt tcgccagcat cttctaccgc caggactacg cctggctggc
     4381 gctggtgatg gccctgcatg cgttcttctg gcacgcggtg ctgccgcagt tcgaggtgat
     4441 caccctggcc cacctgcgcg agcaggcggc gcgctacagc cagatccgcc tgtggggctc
     4501 gatcggcttc atctgcaccg tggtcggcct cggtgcgctg ttcgagcggc tgagtctgga
     4561 tgcctacccc tgggcgctga tcggcatcat gctggccatc gtcctcagca gcctgtggat
     4621 tcccaacgcc cagccgcagg tgcatgccag cgaggccgcg cgcggcggct tcctgcagca
     4681 gctgcgccgt cccggtgtgc cggcgttcta cgccgccgtg gcgctgatgc tgctcagcca
     4741 cggtccgtac tacaccttca tcaccctgca cctggaaagc ctcggctaca gccgtaccca
     4801 gatcggctgg ctgtgggcgc tcggcgtgct ggcggagatc ctcctgttcc tggtgatggc
     4861 gcgcctgctg gcgcgcttct cggtgcgcca ggtgctgctc gccagcctgc tgctggcggc
     4921 actgcgctgg ctgctgctgg gcaacctggc cgatcactgg ccggtgctgc tgctcgcgca
     4981 ggtgatgcat gcggcgacct tcggcagctt ccacgccgcg gccatccatt tcgtgcgcca
     5041 cagcttcgcc gctcgccagc aggggcaggg gcaggcgctg tacgcggcgc tgtccggctc
     5101 cggcgccgcg gtgggcgcgc tctatgcggg ttacagttgg aacagcctcg gcccggcctg
     5161 gaccttcgcc atcgccagcc tggcggcgtt gctcgccgcg ggcatcgtgg tgtggcgcct
     5221 gcgcgagccc gctgccacgt gattccgttt tttgcttcct gacccttcga gaccgcccag
     5281 atgaccgaca agcgcgcact tctcacccga cagatcatcg aggccggccg cttcctttac
     5341 ggccgcggct ggtcgccggc caccagcagc aactactcgg cgcgcctggc tgccgaccag
     5401 gcgctgctca ccgtgtccgg caagcacaag ggccagctga ccgacaacga cgtgctggcc
     5461 accgacctgg ccggcaacag cctggagccg ggcaagaagc cctcggccga gaccctgctg
     5521 cacacccagc tgtacgcctg gcgcgccgac atcggcgcgg tgctgcacac ccactcggtc
     5581 aacgccaccg tgctgtcgcg gctgaccccc ggcgactacc tgctgctgga ggactacgag
     5641 ctgcagaagg ccttcagcgg cgtcaccacc cacgagggcc gggtggtggt gccgatcttc
     5701 gccaacgacc aggacatcgc ccgcctggcc ggccaggtgc agcagtggct ggaggggcac
     5761 gccgactgcg cgggctacct gatccgtggc cacggccttt acacttgggg cgcacagatg
     5821 agcgacgccc tgcgccagat cgaagccttc gagttcctct tcgaatgcga actgaagacc
     5881 cgccaggtgc tgggtcgctg accgaactag ccgaccgaac cagtcggtca cccaaggagt
     5941 ccccatgagc cgcctgagcg tctaccacga gtccaccccg caagtgccga acaaggtgct
     6001 gacccacgcc gaggacatcg ccgccaccct ggccgaggtc ggcgtgcgct tcgagcgctg
     6061 gcaggccagc gcgcccatcg ctcccggcgc cagccaggac gaggtgatcg ccgcctatcg
     6121 ccacgagatc gacaagctga tgagcgagca gggctacgtc accgtcgacg tgatcagcct
     6181 ggaccgcagc catccgcaga aggccgagct gcgcgccaag ttcctcgacg agcacaccca
     6241 cggcgaggac gaggtgcgct tcttcgtcgc cggccgcggc ctgttcaccc tgcacgtcgg
     6301 cgaccaggtg tacgcggtgc tgtgcgagaa gaacgatctg atctcggtac cggccggcac
     6361 cccgcactgg ttcgacatgg gcgaggagcc gcacttcgtc gccatccgcc tgttcaacaa
     6421 ccccgaaggc tgggtcgccc agttcaccgg cgacagcatc gccaagcact tcccgccgct
     6481 ggaggactga cggtgccgat caaagccatc cttaccgaca tcgagggcac caccagcgcg
     6541 gtcagcttcg tcttcgacgt gctgttcccc tacgcccgcg agcacctgcc ggccttcgtg
     6601 cgcgaccatg ccgccgagcc ggcggtggcc gcgcagctgg atgccgtgcg cgccgagagc
     6661 ggcgaggcgg aggccgatgt cgagcgggtc gtcgccatcc tcctcgagtg gatcgccgcc
     6721 gaccgcaagg ccacgccgct caaggcgctg cagggcatgg tctgggaaca gggctaccgg
     6781 gccggccagc tcaagggcca cgtctatccg gatgcggtgg aggcactgcg ccagtggcat
     6841 gccgccggct atgagctgta cgtgtactcc tccggttcga tccaggcgca gaagctgatc
     6901 ttcggctgct ccgaggccgg cgacctgacg ccgctgttct ccggctattt cgacaccacc
     6961 agcggcggca agcgcgaggc cgagtcctac gcgcgcatcg ccgccgccat cggccgcccg
     7021 gccggcgaga tcctgttcct ctccgacgtg gtgcaggagc tggacgccgc gcgcgccgcc
     7081 ggcatggcca ccg
//
LOCUS       DATCUN010000204         5773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_83461, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000204 DATCUN010000000
VERSION     DATCUN010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83461"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /locus_tag="VIO83_08135"
     CDS             <1..1554
                     /locus_tag="VIO83_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEY8331277.1"
                     /translation="GLAPKTARRIAPDGSESDVPLEAVQVGDRLRVRPGEKVPVDGVV
                     LEGHSVVDESMISGEPIPIEKAAGDSVIGATVNGTGALLIEARRVGSDTLLAQIVQMV
                     AEASRSRAPIQNLVDLVAAYFVQAVVGVAILTFIIWSVLGPQPAMAYALINAVAVLII
                     ACPCALGLATPMSIMVASGKGASLGVLFKSAEAIQTLRDVDTLVVDKTGTLTEGRPRL
                     QDVVVAEGFDESEVLRLAATLERGSEHPLAAAIVAGAEERGVEPGRYEDFDSVTGQGV
                     RGRVQGRDVALGNRALMDSRGVELGALAERAEALRGEGKTAMFLAVDGRAAGLVAVAD
                     PIKETTPAAIRSLHEEGIRIVMLTGDSATTARAVAGRLGIDEVMAEVQPDQKAQKIKE
                     LQAQGRFVAMAGDGINDAPALAQAQVGIAMGTGTDVAMESAGVTLVKGDLTGIVRAIR
                     LSRATMRNIRQNLWFAFGYNSLGVPIAAGVLYPFFGILLSPMIAAAAMSFSSVSVIGN
                     ALRLSRVTV"
     gene            complement(1574..2437)
                     /locus_tag="VIO83_08140"
     CDS             complement(1574..2437)
                     /locus_tag="VIO83_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8331278.1"
                     /translation="MNDDNKVLACVDQSRFADYVADYAAWAARRMDAPLEFLHVIDRH
                     PEQASAADHSGAIGIDAQENLLSRLSAQDEARTRNAREQGRIFLNRLRERAIAAGAAR
                     VDVRQRHGELAQTLAEQEHGVRLLILGRRGEAAERTQRDLGRNVEHVVRALHKPILTV
                     TEGFTPPQRVMIAFDGGIVTRRGVAMVVGSPLFRGLPIYLLMSGREKKDARRQLEWAR
                     TTLEAAGFAVSAALIPGDAESVIARTVLAQSIDLLIMGAFGHSPLRSLLFGSKTADLL
                     RSSRIPTLLLR"
     gene            complement(2452..3930)
                     /locus_tag="VIO83_08145"
     CDS             complement(2452..3930)
                     /locus_tag="VIO83_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY8331279.1"
                     /translation="MMKSLRQDWLANVRNDLLAGIVVALALIPEAIAFSIIAGVDPKV
                     GLYASFCIATVIAFAGGRPGMISAATGAMALLMVTLVKEHGLQYLLAATVLTGVLQII
                     AGWLKLGALMRFVSRSVITGFVNALAILIFMAQLPELTNVTWHVYAITAAGLGIIYGF
                     PYITRAVPSPLVAIVVLTGVAIFLGLDIRTVGDMGQLPDSLPIFLLPDVPLNLETLAI
                     IFPVSATLAVVGLLESMMTASIVDELTDSNSNKNRECVGQGVANIASGFLGGMAGCAM
                     IGQSVINVKSGGRGRLSTLAAGVFLLLMVVFIGDWVARIPMAALVAVMIMVSIGTFNW
                     ASIRGLRQHPKSSSVVMLATVVVTVGTHDLAKGVLVGVLLSGFFFAHKVGQILRVASR
                     TEDEGRMRTYTVTGQVFFASAERFINAFDYKEVIEKVRIDVSRAHFWDITAVSALDKV
                     VIKFRREATEVEVIGLNQASATMVDRFAVHDKDGADDLLLGH"
     gene            4254..4526
                     /locus_tag="VIO83_08150"
     CDS             4254..4526
                     /locus_tag="VIO83_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331280.1"
                     /translation="MCRAEGELKNAMDGIFSASLGGAILFGLRRRSNVGCSSRPRLQL
                     AEERQTMHRARRPQRGLMGPLFVTAGHRRGRAFLQAASIGKPLCAG"
     gene            4719..>5773
                     /locus_tag="VIO83_08155"
     CDS             4719..>5773
                     /locus_tag="VIO83_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY8331281.1"
                     /translation="MTTVEGPSTPIGHIVGVQGPVVVIACERLPPLRQALLARFDHQA
                     YLFEVHQHLDEHHLRAVTLHGTSGLTRGMPVYDTGAPLHVPVSADCLGRVLNVFGEPQ
                     DGGLPLAGGAFRNVHQPPPALREVRGVSELLPTGIKVIDLLCPFFRGGKTGLFGGAGV
                     GKTVLIMEFMHAIEAVHQGVSVFAGVGERIREGHELWHDMREAGVLPHALLVFGEMDE
                     SPGVRFRVALTALTYAEYLRDTQHREVLLLIDNIYRMVQAGSEVSSLLGRMPATVGYQ
                     PTLGTEIAEVEDRIASTAAGAVTSVQAVYVPADDLTDPAVSAILGHLDTTVILSRRQA
                     GQGLYPAVDPLRSFSKLA"
BASE COUNT          940 a         1856 c         2041 g          936 t
ORIGIN      
        1 ggtctggcgc ccaagaccgc gcggcgcatc gcgcccgacg gcagcgaatc cgacgtgccg
       61 ctcgaagccg tgcaggtcgg cgaccggctg cgtgtgcggc ccggcgagaa ggtgccggtg
      121 gacggtgtgg tgctggaggg ccacagcgtc gtcgacgagt ccatgatctc cggcgagccg
      181 atcccgatcg agaaggcggc gggtgacagc gtgatcggtg ccaccgtcaa cggcaccggc
      241 gcactgctga tcgaagcccg gcgggtgggc agcgacaccc tgctcgcgca gatcgtgcag
      301 atggtggccg aggccagccg ttcgcgcgcg cccatccaga acctggtgga cctggtggcg
      361 gcgtacttcg tccaggccgt ggtcggcgtc gccatcctca ccttcatcat ctggagcgtc
      421 ttggggccgc agccggccat ggcctatgcg ctcatcaatg cggtggccgt gctgatcatc
      481 gcctgcccct gcgcgctggg gctggccacg cccatgtcga tcatggtggc cagcggcaag
      541 ggcgcctcgc tgggggtgct gttcaagagc gccgaggcca tccagactct gcgcgacgtc
      601 gacaccctgg tggtcgacaa gaccggcacc ctcaccgaag gccggccgcg gttgcaggac
      661 gtggtggtcg ccgagggctt cgacgaaagc gaagtcctgc gtctggctgc gaccctggag
      721 cgcggcagcg agcacccatt ggccgccgcc atcgtcgccg gcgccgagga acgcggcgtc
      781 gagccgggcc gctacgagga tttcgattcg gtgaccggcc agggcgtgcg cgggcgcgtt
      841 cagggccggg acgtggcgct cggcaaccgc gcgctgatgg attcgcgagg ggtcgagctc
      901 ggcgcactgg ccgagcgcgc cgaagcgctg cgcggcgagg gcaagaccgc catgttcctg
      961 gccgtggacg gtcgcgccgc cgggctggtg gcggtggccg atccgatcaa ggaaaccacg
     1021 cccgcggcca tccgctcgct gcacgaggag ggcatccgca tcgtcatgct caccggcgac
     1081 agcgccacca cggcgcgggc agtggccgga aggctcggca tcgatgaggt gatggccgag
     1141 gtgcagccgg atcagaaggc gcagaaaatc aaggagctgc aggcgcaggg ccgctttgtc
     1201 gccatggccg gcgacggcat caacgacgcc ccggcgctgg cccaggccca ggtcggcatc
     1261 gccatgggca ccggcaccga cgtggccatg gagagcgccg gcgtgaccct ggtcaagggc
     1321 gacctgaccg gcatcgtgcg cgccatccgc ctgtcccgcg ccaccatgcg caacatccgc
     1381 cagaacctgt ggttcgcctt cggctacaac agcctgggcg tgcccatcgc cgccggcgtg
     1441 ctgtatccgt tcttcggcat tctgctgtca ccgatgatcg cggcggcggc catgtccttc
     1501 agctcggtgt cggtgatcgg caatgcgctg cgattgagcc gggtgacggt gtagcacccg
     1561 ggcctgcggc gagtcagcgc agcagcaggg tcgggatacg ggacgagcgc agcaggtcgg
     1621 cggtcttgct gccgaacagc aggctgcgca gtggtgaatg gccgaaggcc cccatgatga
     1681 gcaggtcgat ggactgcgcc agcaccgtcc tggcgatgac gctctcggcg tcaccgggga
     1741 tcagggccgc cgagaccgcg aagccggcgg cttccagggt ggtcctggcc cattcgagct
     1801 gccggcgggc gtccttcttt tctctgcccg acatcagcag gtagatcggc aggccccgga
     1861 acaggggact gcccaccacc attgccacgc cccgccgggt gacgatgccg ccatcgaagg
     1921 cgatcatcac gcgctgcggc ggcgtgaagc cttccgtcac cgtcaggatc ggcttgtgca
     1981 gggcgcgcac cacgtgctcg acgttgcggc ccagatcgcg ctgggtacgc tcggccgcct
     2041 caccgcggcg gccgagtatc agcaggcgca cgccgtgttc ctgctcggcc agggtctgcg
     2101 ccagctcgcc gtggcgttgc cgtacatcga cccgcgcggc cccggccgca atggcgcgct
     2161 cccgcagccg gttcagaaag atgcggcctt gctcgcgggc gttcctggtg cgggcttcgt
     2221 cttgcgcgct tagcctggac aacaggttct cttgggcgtc gatgccgatg gcgccgctgt
     2281 ggtctgcagc cgacgcctgc tcggggtggc ggtcgatcac gtgcaggaac tccagcggtg
     2341 catccatgcg ccgggcggcc catgccgcgt agtcggccac gtagtcggcg aaacgggatt
     2401 gatccacgca ggccagcacc ttattgtcgt cgttcatgcc ctggtctcct gtcagtggcc
     2461 cagcagcaag tcgtcggcgc cgtctttgtc atgcaccgcg aagcggtcca ccatcgtggc
     2521 gctggcctgg ttcagaccga tgacttcgac ctcggtcgct tcgcgccgga acttgatgac
     2581 aaccttgtcc agtgcgctga cggcggtgat gtcccagaag tgagcacggc tcacgtcgat
     2641 gcgcactttc tcgatgactt ccttgtagtc gaaggcattg atgaagcgtt ctgccgaggc
     2701 gaagaacacc tgccctgtaa cggtataagt gcgcattcga ccttcgtctt ccgtgcgtga
     2761 ggcgacgcgc agaatctggc ccaccttgtg agcgaagaag aagccggaca gcagcacgcc
     2821 gaccagcacg cccttggcca ggtcgtgcgt gccgacggtg accactacag tggcgagcat
     2881 caccacactg gagcttttgg ggtgctgtcg caggcctcgg atcgaggccc agttgaaggt
     2941 accgatggac accataatca tcaccgcgac cagcgccgcc atcggaatgc gggccaccca
     3001 gtccccgatg aacaccacca tcagcagcag gaacacgcca gcggccagtg tggagagtcg
     3061 accccggcca ccggatttca cgttgatgac cgattggccg atcatggcgc agcccgccat
     3121 gccgccgagg aagccagagg cgatattggc gacaccctga cccacgcatt cgcgattctt
     3181 gttgctgttg gagtcggtca gctcatcgac gatggaggcc gtcatcatcg attccagcag
     3241 gccgaccacg gccagggtgg ccgagaccgg gaagatgatc gccagggttt ccaggttcag
     3301 cggcacatcg ggcagcagga agatcggcag gctgtcgggc agctggccca tgtcgcccac
     3361 ggtgcgaatg tcgaggccga ggaagatcgc cacgccggtc agcacgacga tggcaaccag
     3421 cggcgatggc accgcccggg tgatgtaagg gaagccgtag atgatgccga ggcctgccgc
     3481 cgtgatggca tagacgtgcc aggtgacatt ggtcagctcg ggcaactgcg ccatgaagat
     3541 caggatggcc agcgcattga cgaagccggt gatgaccgag cgagagacaa agcgcatcag
     3601 ggcgccgagc ttgagccagc cggcgatgat ctggagcacg ccggtcagta ccgtggcggc
     3661 cagcaggtat tgcaggccgt gctccttgac gagcgtgacc atcagcagcg ccatcgcgcc
     3721 ggtggcggcc gagatcatgc ccggccgccc gcctgcaaag gcgataacgg tagcgatgca
     3781 gaacgaggcg tagagcccga ccttcggatc gacgccggcg atgatcgaga aggcgatcgc
     3841 ctctgggatc agtgccagcg cgacgacgat gccggccagc agatcgttcc ggacattggc
     3901 gagccagtcc tggcgcaagg atttcatcat tcttccattc cgtttttgat tgccggccag
     3961 tggcggcagt cggggcggac gcaagcgcgt ttccccggtc ggcggaacac agaacgagcc
     4021 ccgagtgatc cggggccagc aagacgcggt gaatcggacg tgcagcctcg tgcgagcgta
     4081 atgtgcgatt gcagctggct cgcagcgaga aagagactta cacgggttga actccgatgg
     4141 ttgcttgctg gtgctttggg aagcgcggat tcattcggcg cttcccgtcc ggggcaggat
     4201 gcccaggcgt gaatccagca ccaagtgctt gattcacgga gtccggcgcg ggcatgtgcc
     4261 gcgccgaagg cgagctgaaa aacgccatgg atggcatttt cagcgcttcc ttggggggcg
     4321 cgattctatt tggtttgcgg cggcgcagca atgtcggctg ttccagccgg cccagactgc
     4381 agttggccga ggagcggcaa accatgcacc gcgcccggcg tccgcagcgc ggtctgatgg
     4441 ggccgttgtt cgtcacggca gggcatcgcc ggggcagggc tttcctgcag gcggcgtcga
     4501 ttggcaagcc gctgtgcgcc gggtgacact gtcaagcgca tgatcgcagt caaaccagcc
     4561 acatgatggc agtcggggga gtctgtagcc tgggttgccc tggcaggcct cgcaggcctg
     4621 cacggcaaat ggaccgggtg gagaccgatg tacacggacg gttgcagcag cttgatgagg
     4681 tgcgcccagg taatgatgtc ttgcccaaag ggttgccgat gacgacggtt gaggggccgt
     4741 ccacccccat cggccatatc gtcggcgtgc aggggccggt ggtggtgatc gcttgcgagc
     4801 gcctgccgcc gctgcgtcag gcgttgctgg cgcgcttcga tcaccaggcc tacctgttcg
     4861 aggtgcacca gcacctggac gagcaccacc tgcgcgcggt gacgctgcac ggcaccagcg
     4921 ggctgacgcg cggcatgccg gtgtacgaca ccggcgcgcc gctgcacgtg ccggtgtcgg
     4981 ccgattgtct gggccgggtg ctgaacgtgt tcggcgaacc ccaggacggc ggcctgccgc
     5041 tggccggcgg cgcctttcgc aatgtgcacc agccgccgcc ggcgctgcgt gaggtgcgcg
     5101 gcgtcagcga gctgctgccg accggcatca aggtgatcga cctgctgtgc ccgttctttc
     5161 gcggcggcaa gaccgggctg tttggcggcg ccggggtcgg caagacggtg ctgatcatgg
     5221 agttcatgca cgccatcgag gcggtgcacc agggcgtgtc ggtgttcgcc ggggtcggcg
     5281 agcgcatccg ggagggccac gagctgtggc acgacatgcg cgaggccggc gtgctgccgc
     5341 acgcgctgct ggtgttcggc gagatggacg agtcgcccgg ggtgcgcttt cgcgtggcgc
     5401 tgaccgcgct cacctatgcc gagtacctgc gcgacaccca gcaccgcgag gtgctgctgc
     5461 tgatcgacaa catctaccgc atggtgcagg ccggcagcga ggtgtcgagc ctgctcgggc
     5521 gcatgccggc cacggtcggc taccagccca cgctgggcac cgagatcgcc gaggtcgagg
     5581 accgcatcgc ctccaccgcc gccggcgcgg tgacctcggt gcaggcggtg tacgtgccgg
     5641 cggacgatct gaccgacccg gcggtgagtg ccatcctcgg acatctggac accacggtga
     5701 ttctgtcgcg ccggcaggcg gggcagggcc tgtacccggc ggtggacccg ctgcgctcgt
     5761 tcagcaagct ggc
//
LOCUS       DATCUN010000205        16751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_8785, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000205 DATCUN010000000
VERSION     DATCUN010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 16751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16751
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8785"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="VIO83_08160"
     CDS             complement(<1..171)
                     /locus_tag="VIO83_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331282.1"
                     /translation="MSNAGNPVRRGRLTVFLGAASGVGKTLAMLQAAQVQLRQGVALR
                     VGLVAASDAAAAA"
     gene            complement(308..763)
                     /locus_tag="VIO83_08165"
     CDS             complement(308..763)
                     /locus_tag="VIO83_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8331283.1"
                     /translation="METSRSREIATMIQPARILTIGIDQPLSQVAAFLAEPRNFPRWA
                     SGLADGLEPAADSPLLPLAGSEWLADTPHGRLRIRFSPANLFGVADHWVHLEDDRIVY
                     VPLRVVANGDGCTVSLTLFRRPEIDEEHFAADCDWVRRDLLELRRVLGG"
     gene            914..2101
                     /locus_tag="VIO83_08170"
     CDS             914..2101
                     /locus_tag="VIO83_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8331284.1"
                     /translation="MPDTSAAPRIPRTVWALGFVSLFMDVSSEMVHSLLPAFMVGTLG
                     IGMLTLGLIEGVSEATALLVKVFSGALSDYLGKRKLLLLFGYGLAALSKPLFPLAQSA
                     ELVFGARLLDRIGKGIRGAPRDALIADVAPQAIRGACFGLRQSMDTLGAFVGPLLAIL
                     LMLWSADDIALVLWFAVLPALLCVAMLAFGVAEPEQAATARRFRSPIHGEALRQFSPA
                     YWWVVAVGAVFTLARFSEAFLVLRAQQLGFPIAWVPLVMVVMAAFYMLSAYPVGKWSD
                     RIGRERLLGVGLVLLVLADLLLAWAESSMTVLIGVALWGLHMGFSQGILATMVADTTP
                     AELKGTAFGLFNLLSGVALLLASGIAGWLWQTYDAATTFHAGALFSVAALALLLGRGA
                     RRP"
     gene            2098..2778
                     /locus_tag="VIO83_08175"
     CDS             2098..2778
                     /locus_tag="VIO83_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8331285.1"
                     /translation="MNLNADFDRRVVIHTANAPWQDSPQAGVQRIPLDRLGEEVARAT
                     SLVRFAAGSRFPAHVHGGGEEILVLEGVFSDETGDYPVGSYLRNPPGSRHTPSSAPGC
                     TLLVKLWQFAPDDHGDLRLDTAAMPWLPGDVEGLTILPLHEHGGVRTALLRLQPGSRC
                     AEQCFAGGEEVFVLDGSLADEAGNYPAGSWLRNPRGSRCRRYSEQGALLYVKTGHIGA
                     PTCWDATR"
     gene            complement(2988..3284)
                     /locus_tag="VIO83_08180"
     CDS             complement(2988..3284)
                     /locus_tag="VIO83_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017475991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY8331286.1"
                     /translation="MNIATTTLDEPKKTCATCQACCCRLEVMLITDTGVPERYIDIDA
                     WGGEVMARLDDGWCAALDRETLLCTIYENRPLICREFEAGEYECLSERKKYLNE"
     gene            complement(3433..3774)
                     /locus_tag="VIO83_08185"
     CDS             complement(3433..3774)
                     /locus_tag="VIO83_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017846747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HEY8331287.1"
                     /translation="MCSAEKVPRKKSVISSFCAWEGDTLVLNILGTPNAKQDAIGKPK
                     GHQLRVSAAAAPVAGRATDHMVRFLAKEFGVTPHDIEVVFGRYNINKQMRIKSPKSLP
                     AVISKHLSQPE"
     gene            complement(3941..4618)
                     /locus_tag="VIO83_08190"
     CDS             complement(3941..4618)
                     /locus_tag="VIO83_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEY8331288.1"
                     /translation="MLRLTTLLAGLVLSLNAFALSIADLSQGDASGGLKDALSQGAKI
                     AVQQLGKPGGFSNDPDVRIGLPGNLGKIASTMKMMGMGNQVEQLEATMNQAAEAAVPH
                     AQALLLDAVKKMTISDAKGILGGPQDAATQYLNRTSREQIRAKFLPIVKQATDQVGLA
                     QQYNAFAGKAASFGVIDAKSANLEGYVAEQALDGLFEVIAQQEASIRENPAGAATSLA
                     KKVFGTL"
     gene            complement(4702..4866)
                     /locus_tag="VIO83_08195"
     CDS             complement(4702..4866)
                     /locus_tag="VIO83_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331289.1"
                     /translation="MLHYEIPYACFAADSGEGRKPTKAVVTRLAELPRHDFSVIDLKN
                     PKDIRSLRDQ"
     gene            4909..6675
                     /locus_tag="VIO83_08200"
     CDS             4909..6675
                     /locus_tag="VIO83_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEY8331290.1"
                     /translation="MVVLVPLAKRLQLGAVLGYLVGGVLIGPSVLGLIAHPASVAQIS
                     ELGVVMLMFIIGLELSPKRLWLMRRAVFGAGLAQVLLCGAGIGAIAVYLFDQRWNSAV
                     ILGLGLALSSTAFALQLLAERHELHSPHGRLAFSILLFQDIAAIPLMALVPLLSGVSR
                     PEMSHGDSLVELLKVVGCVAGLIVGGRYLLRPVLRFVAKSGLQDIFTALALLVVMGSA
                     LLMEWVGMSMALGAFIAGVLLADSEYRHAIEAKIEPFKGMLLGLFFLSVGMSANLGLL
                     LDSFWVVLGLTVLLLLVKLLLASLVGLFPGHLKPLEALRLGVMLASGGEFAFVVFKIA
                     GDHDLFSARVHDLLVLTITLSMALTPLVLLLLSRLIKPAAPEAATAPAETVDAHEARV
                     VIMGMGRVGRIVARILHAQRVPFVAFDTSVEAVELTRNISNLPIYYGNPLHLELLHAA
                     KAEQAEFFIIATDDAQANLETARLIRHRYPHMKVITRARDWQQLHALLDIEAGPVRET
                     FHASLEMGRRVLLGLGLSEEQAELRIQRFRRHDERMLRALYEVASDKDASLKTAHAYY
                     DDLQTLFDNDLREAPISAPEVK"
     gene            6689..8314
                     /locus_tag="VIO83_08205"
     CDS             6689..8314
                     /locus_tag="VIO83_08205"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003227804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEY8331291.1"
                     /translation="MSVSLLSRYACFVGCVLFTLGSLPFLASEPGLWPLTLISGVLSL
                     VGINDLRQTQHAVRRNYPLLGNIRYLVEFIRPEVRQYLLEGDDEQLPFSRAQRSLVYA
                     RAKNESAEKAFGTLSDVYQPGFEFIGHSMLPAEHVDPAGFRVTVGGPQCRQPYSASIF
                     NISAMSFGALSANAIRALNQGAALGGFAHDTGEGSISPYHCEHRGDLIWELGSGYFGC
                     RTADGRFDAQRFALQASDPQVKMIEIKLSQGAKPGHGGILPRHKVTPEIAATRGVAQG
                     EDCVSPARHREFATPIELLQFIARLRELSAGKPVGFKFCLGHPWEFMAIAKAMLETGI
                     LPDFIVVDGKEGGTGAAPREFTDHIGVPMREGLLFVHNTLVGLNLRDQISLGASGKIV
                     SAFDIASVLAIGADWANSARGFMFAIGCIQSQSCHTNKCPTGVATQDPLRQRALVVAD
                     KAARVHNFHRSTLKALAEMLAAAGLEHPAQLEPRHLVRRISTTEIKLFSQLHVFLKPG
                     ELLGEPIEGEFYARMWQMARADSFEPAALAAEG"
     gene            complement(8465..8830)
                     /locus_tag="VIO83_08210"
     CDS             complement(8465..8830)
                     /locus_tag="VIO83_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5064 family protein"
                     /protein_id="HEY8331292.1"
                     /translation="MFEPGHVHMTHPVGSFGLPPYNIDGYYEVRPGAKAGPIVHFRMV
                     GEIDGTPFEEEFEMSHDTVFNFASVMGKLAVKHGMPLNNSPVMRNHKDYDAVFEDIRA
                     KLKVAVGGPLSAHDPNRDA"
     gene            8909..9733
                     /gene="ppk2"
                     /locus_tag="VIO83_08215"
     CDS             8909..9733
                     /gene="ppk2"
                     /locus_tag="VIO83_08215"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019098865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY8331293.1"
                     /translation="MARNKKKKAADLPAEKEKLGRKEYEEELMRLHVELVKLQEWVVA
                     KGLKVCVVFEGRDGAGKGGTIKAITERVSPRVFRVVALPAPSDREKTQMYGQRYLAHM
                     PAAGEVVIFDRSWYNRAGVERVMGFCSEEQAQRFLNVVPLFEKMMVESGIILIKYWLE
                     VSAEEQERRLEDRIDDGRKIWKLSPMDLKSFSRWDEYTRARDEMFAATDTSWAPWYMA
                     RSEEKRRVRLNIISHLLQQVPYEDITRREKIKLPKRSKIGSYKAAPYPFRMVEERF"
     gene            complement(9828..10754)
                     /gene="arcC"
                     /locus_tag="VIO83_08220"
     CDS             complement(9828..10754)
                     /gene="arcC"
                     /locus_tag="VIO83_08220"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEY8331294.1"
                     /translation="MRLVIALGGNALLRRGEAMTAENQRDNVRVACAQIAKVAAGNEL
                     VIAHGNGPQVGLLALQGAAYDTANPYPLDVLGAETEGMIGYMIEQELGNLLPAELPFA
                     TILTQVEVDGADPAFQKPTKPIGPVYAPEEAERLAREKGWSIAPDGNQFRRVVASPRP
                     QRIIEIRPIQWLLEKGAVVICAGGGGIPTLYENGKLRGVEAVIDKDLCSALLAEQLES
                     DLLVIATDVDAAYVDWGKPTQKAIAQAHPDELERLGFAAGSMGPKVQAACEFARHTGK
                     VAVIGSLPEIEAIVQGSAGTRVSTERAGLTFR"
     gene            complement(10768..11778)
                     /locus_tag="VIO83_08225"
     CDS             complement(10768..11778)
                     /locus_tag="VIO83_08225"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY8331295.1"
                     /translation="MAFNMHNRNLLSLMHHSTRELRYLLDLSRDLKRAKYTGTEQQHL
                     GRKNIALIFEKTSTRTRCAFEVAAYDQGASVTYIDPGSSQIGHKESMKDTARVLGRMY
                     DAIEYRGFSQEIVEDLAKYAGVPVFNGLTDEYHPTQMLADVLTMREHSDKPLHEISYA
                     YLGDARNNMGNSLLLIGAKLGMDVRIAAPKALWPSDEHVAACQQFAEETGARITLTED
                     AKAAVKGVDFVHTDVWVSMGEPVEAWGERIQQLLPYQVNMAMMKASGNPRVKFMHCLP
                     AFHNSETKVGQQIAAQYPHLANGIEVTDDVFESPYCIAFDQAENRMHTIKAILVSTLA
                     DI"
     gene            complement(11864..13117)
                     /gene="arcA"
                     /locus_tag="VIO83_08230"
     CDS             complement(11864..13117)
                     /gene="arcA"
                     /locus_tag="VIO83_08230"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEY8331296.1"
                     /translation="MSKLKLGVHSEAGKLHKVMVCSPGLAHLRLTPNNCDELLFDDVL
                     WVSQAKRDHFDFASKMQDRGVDVVEMHNLLTDTVQNQEALKWILDRKLSANQVGVGFG
                     EEMRSWLEGMEPRQLAEFLIGGVSAADLLKGLGDSSVLKMLVDFLGNTSFILPPLPNT
                     QFTRDTTCWIYGGVTLNPMYWPARRQETLLTSAIYKFHPNFVNAEFDIWWGNPDEDHG
                     AATLEGGDVMPVGNGVVLIGMGERSSRQAIGQVAQALFAKGAAERVIVAGLPKSRAAM
                     HLDTVFTFCDRDLVTVFPEVVNQIVPFSIRPDASKPGGMDIRREDKHFIQVVGEALNL
                     KQLRVVETGGDAYEVEREQWDDGNNVVCLSPGVVVGYDRNTYTNTLLRKAGVEVVTIS
                     AGELGRGRGGGHCMTCPIIRDPLDY"
     gene            complement(13238..14899)
                     /gene="sulP"
                     /locus_tag="VIO83_08235"
     CDS             complement(13238..14899)
                     /gene="sulP"
                     /locus_tag="VIO83_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004575691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HEY8331297.1"
                     /translation="MLKQYRLDWLPKDIAAGLVLTSMLVPVGIAYAEASGVPGIYGLY
                     ATIIPLLAYALFGPSRILVLGPDSSLAAPILAVVLPLSGGDPMRAITLASMMAVVSGV
                     VCIVIGLLRLGFITELLSKPIRYGYMNGIALVVLIAQAPKLFGISIDSEGPLLDLWHL
                     AEAIAAGGANWYSFAVGGGSLALILLLKPFKQLPGILIVVILATLVVGFFDLNQFGVK
                     VLGDLPQGLPSFVLPWLSGVDLATVVLGGCAVAIVSFADTSVLSRTYAARTQTKVDPN
                     QEMVGLGAANLAAGLFQGFPISSSSSRTPVAEAAGSKTQLTGVVGALAVAALLLVAPQ
                     LMRYLPNSALAAVVIAAVLGLFEFADLKRIFRIQRWEFWLSMACFAGVAVFGAIPGIC
                     LAVGIAVIEYLWDGWRPHYAILGRVDGVRGYHDTKRYPNARRVPGLVLFRWDAPLFFA
                     NAELFRECLLEAIEESPTPVRRVVVAAEPVTSIDVTSADMLGELCKTLRERGIELHFA
                     EMKDPVKDKFSRFGLLEVFGADIFQPTVGAAVDDYLGDHRVDWTP"
     gene            15655..16266
                     /locus_tag="VIO83_08240"
     CDS             15655..16266
                     /locus_tag="VIO83_08240"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017684806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8331298.1"
                     /translation="MPVAVIFDAFGTLLKIQGGQHPYLRLLKLGKARGRTPRADDIEF
                     LMQQPLTLSGAAQFFEIQAAPDELAALEQVLADEIERVEPYSDGLDAVRLLHAHGVRV
                     GVCSNLAFPYGQAIRRCYPELDAYLLSSELGVMKPDAKIYQHACEQLDVLPHNACMVG
                     DSQRCDRDGPTAFGIEGFYLDRSGGAGDYADLFSFAHDVLNRL"
     gene            complement(16374..16480)
                     /locus_tag="VIO83_08245"
                     /pseudo
     CDS             complement(16374..16480)
                     /locus_tag="VIO83_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543214.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         2713 a         5169 c         5723 g         3146 t
ORIGIN      
        1 cgctgcggcc gccgcgtcag acgctgcgac caggccgacg cgcagggcca cgccctggcg
       61 cagttgcacc tgggccgcct gcagcatggc cagagtcttg cccacgcccg acgcggcgcc
      121 gagaaatacc gtgagccggc cacgacggac cgggttgccg gcattgctca ttgaaccccc
      181 tcgctggatg ccatggataa gtccgctgcc tgctgaaaaa ggaagtgtgc agggcggatt
      241 ttctcccggc gctggcgcgt ggcggcgtaa aaaaacagta aaaacgcctg gctggggccc
      301 ggcgtgctca gccgcccagc acccggcgca gttccagcag gtcgcggcgt acccagtcgc
      361 agtccgctgc gaaatgttct tcgtcgattt cgggccggcg gaacagggtc agcgacaccg
      421 tgcagccatc gccattggcc accacgcgca gcggcacgta gacgatgcgg tcatcctcca
      481 gatggaccca gtggtcggcc acgccgaaga gattggccgg gctgaagcgg atccgcagtc
      541 gcccgtgggg cgtgtcggcc agccactcgc tgccggcgag cggcaggagg gggctgtccg
      601 cggcgggttc caggccgtcg gccaggccgc tggcccagcg cgggaagttg cgcggttcgg
      661 cgaggaaggc tgccacctgg gacaggggct ggtcgatgcc gatggtgagg atgcgtgccg
      721 gctggatcat ggtggcaatc tcgcgggagc gggaagtttc caatcagtct tgcagagcct
      781 tcggcttggc gccattcgtt ggggggctgt ttgggccgcc gccgtggcct gcctctggta
      841 cacgggacta cgctgatcgg cacgagcgtc gcacggcact gccactgctg cgccagatcg
      901 ggaggccaga gccatgccgg acaccagcgc agccccgcgc atcccccgca ccgtctgggc
      961 gctgggcttc gtcagcctgt tcatggacgt gtcgtccgag atggtgcaca gcctgctgcc
     1021 ggcgttcatg gtcggcaccc tgggcatcgg catgctcact ctcggcctga tcgaaggcgt
     1081 cagcgaggcc acggcgctgc tggtcaaagt gttctccggg gcgctcagcg actacctcgg
     1141 caagcgcaag ctcctgctgc tgttcggcta cggcctggcg gcgctgagca aaccgctgtt
     1201 cccgctggcg cagtcggccg agctggtgtt cggcgcgcgg ctgctcgacc gtatcggcaa
     1261 gggcatccgt ggcgccccgc gcgatgcgct gatcgccgat gtcgcgccgc aggcgattcg
     1321 tggcgcctgc ttcggcctgc gccagtcgat ggacaccctg ggcgccttcg tcggcccgtt
     1381 gctggcgatc ctgctgatgc tctggtccgc tgacgatatc gcgctggtgc tgtggttcgc
     1441 cgtgctgccg gcgctgctct gcgtggccat gctggcgttc ggcgtggcgg agccggagca
     1501 ggcggcgacg gcgcggcgct tccgctcgcc gatccatggc gaagccctgc gccagttttc
     1561 cccggcctac tggtgggtgg tggcggtcgg cgcggtattc accctggcgc gcttcagcga
     1621 ggcgttcctg gtgctgcgcg cgcagcagct gggcttcccc atcgcctggg tgccgctggt
     1681 gatggtggtg atggcggcgt tctacatgct gtcggcctac ccggtgggca agtggtcgga
     1741 tcgcatcggc cgcgagcggc tgctgggcgt cgggctggtg ctgctggtgc tggccgatct
     1801 gctgctggcc tgggccgagt cgagcatgac ggtgctgatc ggcgtcgccc tgtgggggct
     1861 gcacatgggc ttcagccagg gcatcctcgc cactatggtg gccgacacca ccccggccga
     1921 actcaagggc accgccttcg gcctgttcaa cctgctcagc ggcgtggccc tgctgctggc
     1981 cagcggcatc gccggctggc tgtggcagac gtatgatgcg gccacgactt tccatgccgg
     2041 cgcgctgttc tcggtggcag cgctggcgtt gctgctgggc cgaggagcga ggcgaccatg
     2101 aacctgaatg ccgatttcga ccggcgcgtg gtgatccaca ccgccaatgc gccgtggcag
     2161 gactcgccac aggcgggcgt gcagcgcatc ccgctggacc gcctcggcga ggaagtggcg
     2221 cgcgccacca gcctggtgcg cttcgccgcc ggcagccgct ttcccgcgca cgtgcacggt
     2281 ggcggcgagg agatcctggt actggagggc gtattcagcg acgaaaccgg cgactacccg
     2341 gtcggcagct acctgcgcaa cccgcctggc agccgccaca cgcccagctc ggcgccgggc
     2401 tgcaccctgc tggtcaagct ctggcagttc gccccggacg accacggcga cctgcgtctg
     2461 gataccgccg ctatgccctg gctgcccggc gacgtcgaag gactgacgat cctgccgctg
     2521 cacgagcacg gcggcgtgcg taccgcgctg ctgcgcctgc agcccggcag ccgttgcgca
     2581 gaacaatgct ttgcgggcgg cgaggaagtc ttcgtgctgg acggcagcct ggccgacgag
     2641 gccggcaact acccggccgg cagctggctg cgcaacccgc gcggcagcag gtgccgccgc
     2701 tattcggagc agggcgcctt gctctatgtg aagaccgggc atatcggcgc gccgacctgc
     2761 tgggacgcga cacggtagcg ggccgttcgc ggcgcgcgca ggacactgcc catctcgcag
     2821 gggccaggca agagcgaaga acagcgtagg cgcgatgtcg ggtattggcg ggagacgggg
     2881 cgatgggggg atgagcgggc cgggttgttt catctcaagc cactttggca tgtggctgat
     2941 ccagacgaac tcggaaagtt tttcgtactt gtccgcgaag taaaacatta ctcgttcaaa
     3001 tattttttcc gttcgctaag gcactcgtat tcgcccgctt caaattcccg gcaaataagc
     3061 gggcgatttt catagatggt gcacagcaag gtttcgcggt cgagcgcggc acaccagcca
     3121 tcgtccaggc gcgccatgac ctcgccgccc cacgcatcga tatcgatata gcgttccggg
     3181 acgccggtgt ccgtgatcag catgacctcc agacggcaac agcaggcctg gcaggttgca
     3241 caggttttct tgggctcatc cagtgtcgtt gttgcaatat tcatgatttg ctgtgttgga
     3301 atcaaattat tgttcaattg tagcgataat ggcgaatgtg cgccatttcg agtcgtatgg
     3361 ctgggttttg gtaatgatta cttgggtggt ctggtcattg tggggacttt ggcctggtca
     3421 ggtttcaggt gcttattctg gttgggataa gtgtttgctg attaccgctg gcaggctctt
     3481 gggtgatttg atgcgcattt gtttgttgat gttgtatctg ccaaatacaa cttcgatgtc
     3541 atggggggtg acgccaaatt ccttcgccag gaagcgaacc atatgatcgg tagcccggcc
     3601 cgcaacgggt gcggcggcgg cactgacgcg tagctgatgg cctttgggct tgccgatggc
     3661 atcctgtttg gcgttgggtg tgccgagaat gttgagcacc aacgtatcgc cctcccacgc
     3721 acagaaagaa ctgattaccg acttcttgcg cggcactttt tcagcggagc acattgactt
     3781 tttcctcttg ggttttgcgc cgtccggaca gcaagaagcc cgcacggagc gggcttcttg
     3841 tctggggtgc taatccttca gctgggtcga tagtgccggg cggcgtgtga attaaacgtg
     3901 aaaagccgca gccgcctgtc cgtacccgtg gctgggttgc ttacagcgtg ccgaacacct
     3961 tcttggccag gctggtggcg gcaccggccg ggttctcgcg gatgctggct tcctgctggg
     4021 cgatcacttc gaacaggccg tcgagcgcct gctcggccac gtagccttcg aggttggcgc
     4081 tcttggcgtc gatcacgccg aagctggcgg ccttgccggc gaaggcgttg tactgttggg
     4141 ccaggccgac ctggtcggtg gcctgcttga cgatgggcag gaacttggcg cggatctgtt
     4201 cgcggctggt gcggttgagg tactgggtgg cggcgtcctg cgggccgccg aggatgccct
     4261 tggcatcgct gatggtcatc ttcttcaccg cgtccagcag cagggcctgg gcgtgcggca
     4321 cggcggcttc ggcggcctgg ttcatggtgg cttccagctg ttcgacctgg ttgcccatgc
     4381 ccatcatctt catggtgctg gcgatcttgc cgaggttgcc cggcaggccg atgcgcacgt
     4441 ccggatcgtt gctgaaaccg ccgggcttgc ccagttgctg tacggcaatc ttcgcgccct
     4501 ggctgagggc gtccttgagg ccgccgctgg cgtcgccctg gctcaggtcg gcaatcgaca
     4561 gggcgaaggc attgaggctc agcacaaggc cggcgagcag ggtagtgaga cggagcatgg
     4621 cggcaatcct attgcggggg aaaacctgat cttagcagcg ccccggccag gcgcccacgc
     4681 gctggcgcgg catgctttgt cttattggtc acgcaagctt cggatatcct tggggttttt
     4741 caggtctatg acgctgaagt cgtgacgcgg cagctcggcc agccgcgtca cgacggcttt
     4801 cgttggtttt cggccttcgc cgctgtcggc ggcgaagcag gcataaggaa tttcgtagtg
     4861 gagcatgggg cgggcctgtt gcaggccagt gttgtctgtc ttctcaccat ggtggtgctg
     4921 gttccactgg ccaagcgcct gcagctcggc gccgtgctgg gctatctggt cggcggggtg
     4981 ttgatcggtc ccagcgtgct ggggctgatc gcccatcccg ccagtgttgc ccagatctcc
     5041 gagctgggcg tggtgatgct gatgttcatc atcggcctgg aattgtcgcc caagcggctg
     5101 tggctgatgc gccgcgcggt gttcggcgcg gggctggccc aggtgttgct ctgcggggcg
     5161 ggcatcggcg cgatagcggt gtatctgttc gatcagcgct ggaacagcgc ggtgattctg
     5221 ggtctggggc tggcgctgtc ctcgacggcc ttcgcgctgc agttgctggc cgagcgccat
     5281 gagctgcaca gtccgcatgg ccggctggcg ttttccatcc tgctgttcca ggacatcgcg
     5341 gccattccgc tgatggccct ggtgcccctg ctcagtgggg tcagccggcc ggagatgagt
     5401 cacggcgaca gtctcgtcga actgctcaag gtggtgggct gcgtggcggg tctgatcgtc
     5461 ggcgggcgct atctgctgcg tccggtgttg cgcttcgtcg ccaagtccgg gctgcaggac
     5521 atcttcactg cactggcgct gctggtggtg atgggcagcg ccctgctgat ggagtgggtc
     5581 ggcatgtcca tggcgctggg ggcgttcatc gccggcgtgc tgctggccga ctcggaatac
     5641 cggcacgcga tcgaagccaa gatcgagccg ttcaagggca tgttgctggg gctgttcttc
     5701 ctcagcgtgg gcatgagcgc caacctgggg ctgctgctgg actcgttctg ggtggtgctg
     5761 ggcctgacgg tgctactgct gctggtcaag ctgctgctgg ccagtctggt cggtctgttc
     5821 cccgggcacc tgaagccgct ggaggccctg cgtctggggg tgatgctcgc ctctggtggc
     5881 gaattcgctt tcgtggtgtt caagatcgcc ggtgaccatg atctgttcag cgcgcgggtg
     5941 catgacctgc tggtgctgac catcaccctg tcgatggccc tgacgccgct ggtgctcctg
     6001 ctgctgtcgc ggctgatcaa gcccgctgcg cccgaggccg ccacggcgcc ggcggagacg
     6061 gtcgatgccc atgaggcgcg cgtggtgatc atgggcatgg ggcgggtcgg gcgtatcgtc
     6121 gcgcgcatcc tgcatgcgca gcgcgtgccc ttcgtggcgt tcgatacctc ggtcgaggcc
     6181 gtcgagctga cccgtaacat cagcaatctg ccgatctatt acggcaatcc cctgcacctg
     6241 gaactcctcc atgcggccaa ggccgagcag gcggagttct tcatcatcgc caccgatgac
     6301 gcgcaggcca accttgaaac cgcccggctg atccgccatc gctatccgca catgaaggtc
     6361 atcacccgcg cccgtgactg gcagcaactg cacgcgctgc tggatatcga ggccggaccg
     6421 gtgcgcgaga ccttccacgc cagcctggag atgggccggc gggtcctgct gggcctgggg
     6481 ttgagcgagg agcaggcgga gttgcgcatc cagcgcttcc gccggcatga cgagcggatg
     6541 ctgagagcgc tgtacgaggt cgccagcgac aaggatgcca gcctcaagac cgcccatgcc
     6601 tactacgacg atctgcaaac gctgttcgac aatgatctgc gggaagcgcc gataagcgcg
     6661 ccggaagtca aataaagggg aagtgcgcat gagcgtgtcg ttgctgagtc gttatgcctg
     6721 tttcgtcggc tgcgtgttgt tcaccctcgg cagcctgccg tttctggcca gcgagccggg
     6781 actgtggcca ctgaccctga tcagcggcgt gctcagcctg gtggggatca acgacctgcg
     6841 ccagacgcag catgcggtgc ggcgcaacta tccgctgctc ggcaatatcc gctacctggt
     6901 ggagttcatc cgtccggaag tgcgccagta cctgctggaa ggcgatgacg agcagctgcc
     6961 gttctcccgc gcgcagcgct cactggtcta tgcgcgggcg aagaacgaga gtgcggaaaa
     7021 ggccttcggc acgctcagcg acgtctacca gcccggcttc gagttcatcg gccactccat
     7081 gctgccagct gaacatgtcg atccggcggg ctttcgcgtc accgtgggcg ggccgcagtg
     7141 ccggcaaccc tactctgcgt cgatcttcaa catctcggcg atgagcttcg gcgcgctgag
     7201 cgccaacgcc atccgcgcgc tgaaccaggg cgccgcgctc ggcggcttcg cccacgacac
     7261 cggcgagggc agcatcagcc cgtaccactg cgagcaccgc ggcgacctga tctgggagct
     7321 gggcagcggc tacttcggct gccgtacggc ggacggccgt ttcgatgcgc agcgctttgc
     7381 cctgcaggcc agcgatccgc aggtgaagat gatcgagatc aagctgagcc agggcgccaa
     7441 gcccggccat ggcggcatcc tgccccgaca caaggtcacc ccggaaatcg ccgccacccg
     7501 cggcgtcgcc cagggcgagg actgcgtgtc accggcgcgc caccgcgagt ttgctacgcc
     7561 gatcgagctg ctgcagttca tcgcccgtct gcgcgagctg tccgctggca agccggtcgg
     7621 cttcaagttc tgcctcggcc atccgtggga gttcatggcc atcgccaagg ccatgctgga
     7681 gacgggcatc ctgcccgact tcatcgttgt cgacggcaag gagggcggca ccggtgcggc
     7741 gccacgcgaa ttcaccgacc atatcggcgt gccgatgcgc gagggcctgc tgttcgtgca
     7801 caacaccctg gtcggcctga acctgcgcga ccagatcagc ctcggggcca gcggcaagat
     7861 cgtcagcgcc ttcgacatcg ccagcgtgct ggccatcggc gccgactggg ccaactcggc
     7921 gcgcggcttc atgttcgcca tcggctgcat ccagtcgcaa agctgccata ccaacaagtg
     7981 cccgaccggc gtggcgaccc aggacccgct gcgccagcgc gcgctggtgg tggcggacaa
     8041 ggccgcgcgg gtgcacaact tccaccgcag cacgctcaag gcgctggccg aaatgctcgc
     8101 cgccgccggc ctcgagcatc cggcccagct ggagccccgg catctggtac ggcgtatctc
     8161 caccacggaa atcaagttgt tttcccagct gcacgtgttc ctcaagccgg gtgagctgct
     8221 cggcgagccg atcgagggcg agttctacgc gcgcatgtgg cagatggcgc gggccgacag
     8281 tttcgagccc gcggcgctgg ccgccgaggg gtagggccgc agcgcaagag cccgtactgg
     8341 gcgggctctc tggcccgttc atcctggccg tggcctgcgg gctaccccgg aaatgggcag
     8401 gcgtctctgc gcccggcagc ccagggccgg gcattgaact gtccaggctc aggccaaccg
     8461 cgggtcaggc atcccggttg gggtcatggg cgctcagcgg cccgccgacg gccactttga
     8521 gcttggcgcg gatgtcctcg aacaccgcgt cgtaatcctt gtggttgcgc atgaccgggc
     8581 tgttgttgag tggcatccca tgcttgaccg cgagcttgcc catcacactt gcgaagttga
     8641 acacggtgtc gtgggacatc tcgaactctt cctcgaaagg cgtgccgtcg atctcgccaa
     8701 ccatgcggaa gtgcacgatc ggccctgcct tggcgccagg gcgcacttcg taatagccat
     8761 cgatgttgta gggcggcagg ccgaaactgc ccaccggatg ggtcatatgc acgtgaccgg
     8821 gctcgaacat tactacactc ctaacattgg gaactgcagg cagtcctgag tgtaggtcgt
     8881 gtattcatgc ggcaaaggag gttggcaaat ggctaggaac aagaagaaaa aagcagcgga
     8941 tctaccggct gagaaggaaa aactaggtcg taaggagtac gaagaagagc tgatgcggct
     9001 gcacgtcgag ctggtcaagc tgcaggagtg ggtggtggcc aagggcctga aggtgtgcgt
     9061 cgtcttcgag ggccgcgatg gcgcaggcaa gggcggcacc atcaaggcga tcaccgagcg
     9121 ggtcagcccg cgggtgttcc gcgtggtggc gctgccggcg cccagcgacc gggaaaagac
     9181 ccagatgtat ggacagcgct acctggccca catgccggcg gctggcgagg tggtgatctt
     9241 cgaccgcagc tggtacaacc gcgccggcgt cgagcgggtg atgggcttct gcagcgagga
     9301 gcaggcccag cgttttctca acgtggtgcc gctgttcgag aagatgatgg tcgagtccgg
     9361 gatcatcctg atcaagtact ggctcgaggt gagtgccgag gagcaggagc gccgcctgga
     9421 agatcgcatc gacgacggac gcaagatctg gaagctgtcg ccgatggacc tgaaatcctt
     9481 cagccgctgg gacgagtaca cccgtgcccg cgacgaaatg ttcgcggcca ccgacacctc
     9541 ctgggcgccc tggtacatgg cgcgctccga ggagaagcgg cgcgtgcgcc tgaacatcat
     9601 cagccacctg ctccagcagg ttccctacga ggacatcacc cggcgggaga agatcaagct
     9661 gcccaagcgc tccaagatcg gcagctacaa ggctgcgccg tatccattca ggatggtcga
     9721 ggagcgcttc tgaagagccg gaaaccggta tcggacgcac cttttccgct agatcaagac
     9781 aaaggcccgc ggttgtacca agggcctttg ccgtcatgga ttggcgctca gcggaaggtc
     9841 agtcccgctc gctccgtgct gacgcgggta ccggcgctgc cctggacgat cgcctcgatc
     9901 tccggcagcg agccgatcac cgccaccttg ccggtatggc gggcgaactc gcaggcggcc
     9961 tggaccttcg gacccatgga accggcggcg aagccgaggc gctccagctc gtccgggtgc
    10021 gcctgggcga tggctttctg cgtcggcttg ccccagtcga cataggccgc gtcgacgtcg
    10081 gtggcgatca ccagcaggtc gctttccagc tgttcggcca gcagggccga gcagaggtcc
    10141 ttgtcgatca ccgcctccac gccgcgcagc ttgccgttct cgtacagggt cgggatgccg
    10201 ccgccaccgg cgcagatcac caccgcgccc ttttccagca gccactggat cggacggatc
    10261 tcgatgatgc gttgcggccg cgggctggcc accacgcgac ggaactggtt gccatcgggg
    10321 gcgatgctcc agcctttctc cctggccaga cgctcggctt cttccggcgc gtagaccggg
    10381 ccgatcggct tggttggttt ctggaacgcc gggtcggcgc cgtccacctc gacctgggtg
    10441 agaatggtgg cgaagggcag ctcggccggc agcaggttgc ccagctcctg ttcgatcatg
    10501 tagccgatca tgccttcggt ctcggcgccc agcacgtcca gcgggtaggg gttggcggta
    10561 tcgtaggccg cgccctgcag ggccagcagg ccgacctgcg ggccgttgcc gtgggcgatc
    10621 accagttcgt tgccggcggc caccttggcg atctgcgcgc aggcgacgcg cacgttgtcg
    10681 cgctggttct ccgcggtcat ggcctcgcca cggcgcagca gggcgttgcc gcccagggcg
    10741 atgactagtc gcatgcgggt gctcctctca gatgtccgcc agggtcgata ccaggatcgc
    10801 cttgatggtg tgcatgcggt tttccgcctg gtcgaaggcg atgcagtagg gcgactcgaa
    10861 gacgtcgtcg gtcacctcga tgccattggc caggtgcgga tactgcgcgg cgatctgctg
    10921 gccgaccttg gtctcgctgt tgtggaaggc cggcaggcag tgcatgaact tcacccgcgg
    10981 gttgccgctg gccttcatca ttgccatgtt gacctggtag ggcagcaatt gctggatgcg
    11041 ttcgccccag gcttccaccg gctcgcccat cgacacccac acatcggtgt ggacgaagtc
    11101 cacgcccttc accgcggcct tggcgtcttc ggtgagggtg atgcgcgcgc cggtttcctc
    11161 ggcgaactgc tggcaggcgg cgacgtgctc gtcgctcggc cacagcgcct tgggtgcggc
    11221 gatgcgcacg tccatgccga gcttggcgcc gatcagcagc agcgagttgc ccatgttgtt
    11281 gcgggcgtcg cccaggtagg cgtagctgat ctcgtgcagc ggcttgtcgc tgtgctcgcg
    11341 catggtcagc acgtcggcga gcatctgggt cgggtggtat tcgtcggtca ggccgttgaa
    11401 caccggcaca ccggcgtact tggccagatc ctcgacgatc tcttggctga agccgcgata
    11461 ctcgatggcg tcgtacatgc ggccgagcac ccgggcggta tccttcatgc tttccttgtg
    11521 gccgatctgc gaggagcccg gatcgatgta ggtgacgctg gcgccttggt cgtaggcggc
    11581 cacctcgaag gcgcagcggg tgcgggtgga ggtcttctcg aagatcagcg cgatgttctt
    11641 gcgcccgagg tgctgctgtt cggtgccggt gtacttggcg cgcttgaggt cgcgcgacag
    11701 gtcgaggagg taacgcagct cgcgggtgct gtggtgcatc aggctgagca ggttgcggtt
    11761 gtgcatgttg aacgccatga cggattccct ctggtgaaat cggagctgtg ctgtatgaac
    11821 gcgggcgagc gcggcgacct cacccggccg ccgcgctggg gcatcagtag tcgagcgggt
    11881 cgcggatgat cgggcaggtc atgcagtggc caccgccacg gccgcggccc agctcgccgg
    11941 cgctgatggt gaccacttcc accccggcct tgcgcagcag ggtgttggta taggtgttgc
    12001 ggtcgtagcc gaccaccacg ccgggcgaca ggcagaccac gttgttgcca tcgtcccact
    12061 gctcgcgttc cacctcgtag gcgtcgccgc cggtctccac cacgcgcagc tgcttcaggt
    12121 tgagggcctc gccaaccacc tggatgaagt gcttgtcctc gcggcggatg tccatcccgc
    12181 ccggcttgct ggcgtcgggg cggatgctga acggcacgat ctggttgacc acttccggga
    12241 agacggtgac caggtcgcga tcgcagaagg tgaacacggt atccaggtgc atggcggcgc
    12301 gcgacttggg caggccggcg acgatcaccc gctcggctgc gcccttggcg aacagggcct
    12361 gagccacctg gccgatggcc tggcgcgagc tgcgctcgcc catgccgatc agcaccacgc
    12421 cgttgccgac cggcatcacg tcgccgccct cgagggttgc ggcgccatgg tcctcgtccg
    12481 ggttgcccca ccagatgtcg aattcggcat tcacgaagtt cgggtggaac ttgtagatcg
    12541 cgctggtcag cagggtttcc tggcggcgcg ccggccagta catcgggttg agggtcacgc
    12601 cgccgtagat ccagcaggtg gtgtcgcggg tgaactgggt gttgggcagc ggcggcagga
    12661 tgaagctggt gttgcccagg aagtcgacca gcatcttcag cacgctgctg tcgccgaggc
    12721 ccttcagcag gtcggcggcc gatacgccac cgatcaggaa ctcggccagc tgacggggtt
    12781 ccatgccttc cagccagctg cgcatttcct cgccgaagcc aacgccgacc tggttggccg
    12841 acagcttgcg gtcgaggatc cacttcagcg cttcctggtt ctgcacggtg tcggtcagca
    12901 ggttgtgcat ctccaccacg tcgacaccgc ggtcctgcat cttgctggcg aagtcgaagt
    12961 ggtcgcgctt ggcctgggac acccagagca cgtcgtcgaa cagcagttcg tcgcagttgt
    13021 tcggagtcag gcgcaggtga gccaggcccg gcgagcagac catcaccttg tgcagcttgc
    13081 cggcttcgga atgaacgccg agtttgagct tggacatcgg gaactccttg cgtggatgct
    13141 gttgattgag tgtagaaagc tcaggcggcg atctcgctgc cggtgacagc ctgcgcatcc
    13201 aggcgggctg cgctcagtcg cccaggattc agtgtgctca gggtgtccag tccacccggt
    13261 gatcgccgag gtagtcatcc acggcggcgc ccacggtggg ctggaagatg tctgcgccga
    13321 acacttccag cagtccgaag cggctgaact tgtccttgac cgggtccttc atctccgcga
    13381 aatgcagctc gatccctcgc tcgcgcaggg ttttgcacag ctcaccgagc atgtccgcgg
    13441 aggtgacgtc gatgctggtg accggctcgg ccgccaccac cacccggcgt accggcgtcg
    13501 gcgactcctc gatggcctcc agcaggcatt cacggaacag ctcggcgttg gcgaagaaca
    13561 gcggcgcgtc ccagcggaac agcaccagac cgggcacccg gcgggcgttc ggatagcgct
    13621 tggtatcgtg atagccacgc acgccgtcga cgcgaccgag aatggcgtaa tgcgggcgcc
    13681 agccgtccca caggtactcg atcacggcga tccccaccgc caggcagatg ccggggatcg
    13741 cgccgaacac ggcgaccccg gcgaagcagg ccatcgacag ccagaactcc cagcgctgga
    13801 tgcggaagat gcgcttgagg tcggcgaact cgaacaggcc gagcaccgcg gcgatcacca
    13861 ccgcggccag cgcgctgttg ggcaggtagc gcatcagttg cggcgcgacc agcagcagcg
    13921 ccgccaccgc cagggcgccg accaccccgg tcagctgggt cttggagcca gcggcctcgg
    13981 ctaccggcgt gcgcgaggag ctgctgctga tgggaaagcc ctggaacagc ccggcggcca
    14041 ggttggcggc gcccaggccg accatttcct ggttggggtc gaccttggtt tgcgtgcgcg
    14101 cggcataggt gcgcgacagc acgctggtgt cggcgaagga gacgatggcg accgcgcagc
    14161 cgccgagcac cacggtggcc aggtccacgc cgctgagcca gggcaggacg aagctgggca
    14221 gcccctgggg caggtcgccc agcaccttga cgccgaactg gttcaggtcg aagaagccaa
    14281 ccaccagggt cgcgaggatc accacgatga ggatgccggg cagctgcttg aacggcttga
    14341 gcagcaggat cagcgccagg ctgccgccgc ccacggcaaa gctgtaccag ttggccccgc
    14401 cggcggcgat ggcctcggcc aggtgccaga ggtcgagcag ggggccttcg ctgtcgatgg
    14461 agatgccgaa cagcttggga gcctgagcga tcagcacgac cagggcgatg ccgttcatgt
    14521 agccgtagcg gatcggcttg gacagcagtt cggtgatgaa gcccaggcgc agcaggccga
    14581 tgacgatgca caccacgccg gagaccacgg ccatcatgct ggccagggtg atggcgcgca
    14641 tcgggtcgcc gccggagagc ggcagcacca cggcgaggat gggcgcggcg agcgacgagt
    14701 ccgggccgag caccaggatg cggctggggc cgaacagggc gtaggccagc agcggaatga
    14761 tggtggcata caggccgtag atgcccggca cgccggaggc ctcggcgtag gcaatgccga
    14821 ccggcaccag catggaggtg agcaccaggc cggcggcgat gtccttgggc agccagtcga
    14881 ggcggtactg cttgagcatc agcagaccgg gcagccagcg cagccaggag gtcttggcgg
    14941 gcgaggggga cgggttgtcg ggcgtcgtgg gctctggtgg catggcaaat cgtccttatc
    15001 aaaggccggg agctggctca ggaggtcggg ctactgggag tctgggagtc aagaggaagg
    15061 ctaggtcaag aaacgtctac atgccctgct cagggtggcc gggtgctgct ggccgtctac
    15121 gacatgggga gtgagtctcg agccgcttga agcgggcatc ttgctgggga aggggcaggt
    15181 ataccaagga tatacggggg caggcgggaa cctgcgggca caaaaaaacc ggctacaggg
    15241 gccggtttct ctgggtttcg tcgacatgtc ggagcatgtg gaaacaagta tgtggagcgg
    15301 gcgatgggat cggaatccca tttgatcaag ctcgctacaa tacagcttta cgctagatat
    15361 atggcgcctt cctgttgatt ggaaagcggc gtcttagcta ttggtgaaga tactcagctt
    15421 tcgagggatg tcaagaggga ttttgcggag gttcaagccc gatccaggcc ttgactgggg
    15481 agggcggcca ccggacgcgg gttcatttgc ccgggacgac gagttgtcca ggcacctgtc
    15541 catgatagcg agggggtagg gtggcctggc ccttggctct tcggaacgaa tgacatcccg
    15601 aagcgttcat gccagtctga cacgcaccag cagatcatca ggaagttctc gtccatgccc
    15661 gtggctgtca tctttgatgc gtttggcacc ctgctcaaga ttcaaggcgg ccagcatccc
    15721 taccttcgac tgctcaagct tggcaaggct cgcggccgta ctccgcgtgc agacgacatt
    15781 gaatttctga tgcagcagcc gctgacgctg tcgggggccg ctcagttttt tgagattcag
    15841 gctgcgcccg atgagctagc agcgctggag caagtgctcg ctgacgagat agagcgcgtc
    15901 gagccctact cggacgggct tgacgcagtg cgtctgttgc acgctcatgg tgtacgggtg
    15961 ggcgtttgct ccaatctagc ttttccctat ggccaagcga tcaggcgctg ctatcccgaa
    16021 ctcgatgcat acctgctcag ttccgagctg ggtgtcatga agcctgacgc gaaaatctac
    16081 cagcatgcct gcgaacagct cgatgtcttg ccccataacg cttgcatggt cggggactcg
    16141 cagcgctgtg accgggatgg cccgacagcg tttgggatcg aggggtttta tctggatcgc
    16201 agcggtgggg caggggatta cgccgacttg ttcagcttcg cccatgatgt gctgaacagg
    16261 ctgtagcgcg aggcaggatc attttcagtc gacggccccg cctcctaatg ggcgcggggg
    16321 cgtcagcccc gtcaacgaat tccgctgctc agactgttag cctatgaaaa cggaattccc
    16381 ccaaccgtta tccgcagatc tggcttgtgg taaatcgctt gctgaagctg aattttttca
    16441 gcttcgacca aagctcgtca ctgagcatca atcggggcat tgcaaacccg cagggttatt
    16501 ggtgtaggaa cctcaattct gcgggtttgc ttctcttcct caagacttcc agcgcgaaac
    16561 gtcaacagga cttaagccat caagacccag ggcggtgagg atttctaggg ctcgcttccc
    16621 ggttaatcgc aatgctgctc cgcttaggtg agcagcattg caccacaggt cttctacggt
    16681 aaattacatt cagatgtgac gtagaaaacc cgcatcacaa ccaggttccg ccctggcgtt
    16741 catctggctc g
//
LOCUS       DATCUN010000206         8001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_102964, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000206 DATCUN010000000
VERSION     DATCUN010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8001
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102964"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VIO83_08250"
     CDS             <1..375
                     /locus_tag="VIO83_08250"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8331299.1"
                     /translation="LLGRRPRQPLGFQVGMDLSGAAGRAWSNMAHCVFGELQRAPQLF
                     EAPLIRTQFEQTLMTTLLQWQPNNLQGEGPEHRNKVLPRYVKLAEEYLQAHPEQPITA
                     ETLATLTGVSARCTAGSRAFSA"
     gene            372..563
                     /locus_tag="VIO83_08255"
     CDS             372..563
                     /locus_tag="VIO83_08255"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8331300.1"
                     /translation="MSPMRYLRDIRMERVRQDLLDPHKPRSVTAIATHWGFFQLGRFA
                     TEYRHRFAEAPRETILKRH"
     gene            744..1973
                     /locus_tag="VIO83_08260"
     CDS             744..1973
                     /locus_tag="VIO83_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009868033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="styrene monooxygenase/indole monooxygenase
                     family protein"
                     /protein_id="HEY8331301.1"
                     /translation="MRKITIIGAGQAGLQLGIGLLGQGYAVRIVSNRSPEQIGAGRVM
                     SSQSMYDMALAFERELGIAFWDDECPPTDGVHMRAGSGDQLLIDWRGLMGAPGQSVDQ
                     RIKMPVWMAEFQRRGGELVIEDAGIPELERYAQTSDLVIVSTGKGEIGKLFERDNDLS
                     AFDAPLRTISLTYVHGMKPREDFSALNISINPGIGELVNFPGMTLSGPCDIINLECIP
                     NGPMDRWHEVQSQEEHLALAVQMIEEYFPWEAERCQNLRLTDDQCTLVGRITPTVRKP
                     VGYLPSGRTVLGMADVLVLNDPITGQGSNNASKCANVYMQCILDQGEKAFDDAWKNAT
                     FARYWDYAQWVAKFTNTHLLPPTEPVLKVLTACGRLPELAAHFSNAFNDPKMLTPWYY
                     DSAEAERFIASFDSKAA"
     gene            1983..3188
                     /locus_tag="VIO83_08265"
     CDS             1983..3188
                     /locus_tag="VIO83_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009868034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEY8331302.1"
                     /translation="MTIKKPKRHGLRIAASTVALCALPWSIAQATQGTMPHGYGVKSE
                     GMGGVVMALPQDALVGASNPAGMVQVGDRLDLGVAFLKVDQGAEFAGTDYEASHDRDL
                     YVIPQLGLNKMLDERSSIGLSLVGNGVGTGYASSDNIGGLQSPGSEFQQMVGTLSYSR
                     QLNEVHSVGVGLLLIRQALDIDGTASIGLPEGRDVAYGTGLRLGWMARLNDEITFGAS
                     YATRGYMSKMDRFENLLAEDGDMDMPETYGMGLAYQKDRLTLGLDVQRIMWADVKSLA
                     NPGVGSATGAPGSSNGPGFGWQNQTVWRVGAAYDVLPELTLRAGYNHGSKLIDSQDTY
                     LGLLAPSANRRHATAGATYRLDNGHELSMAYARSFKEKVKGDGPGPDGLTNVYMGQHW
                     LSLSYGIAF"
     gene            3203..3697
                     /locus_tag="VIO83_08270"
     CDS             3203..3697
                     /locus_tag="VIO83_08270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY8331303.1"
                     /translation="MIEAKQLRQALSNFATGITIVTTQDDRQQPIGVTASSFNSVSLT
                     PPLVLWSIAKSAYSYASFAQAEHFAVHVLGADQAALSDRFARASTDKFAGLDTARGVG
                     DVPLIHGCMARFECATEHRYEGGDHMIIVGRVLNLNLSERPCDPLLFFKGRYATLDGG
                     DQAA"
     gene            complement(3950..4026)
                     /locus_tag="VIO83_08275"
     tRNA            complement(3950..4026)
                     /locus_tag="VIO83_08275"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3990..3992),aa:Arg,seq:ccg)
     gene            4241..5695
                     /gene="gabD"
                     /locus_tag="VIO83_08280"
     CDS             4241..5695
                     /gene="gabD"
                     /locus_tag="VIO83_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY8331304.1"
                     /translation="MELKDPDLFRQQAFVNGQWCEADHGARTDIHNPASGEKLGSVPN
                     MGRAETRRAIAAAQAAQPAWRALTAKERSTRLRKWYELIMANQDDLAQIMTAEQGKPL
                     AEARGEVAYGASFIEWFAEEAKRAYGDVIPAHAADKRILVQKEPVGVTAAITPWNFPS
                     AMITRKAGPALAAGCAMVLKPAPQTPFSALALAELAQRAGIPAGLFSVLPADVETSRE
                     VGAELCENPIVRKLSFTGSTAVGIQLMAQCAPTLKKLSLELGGNAPFIVFADADLDAA
                     VEGAMISKYRNAGQTCVCANRIYVQESVYDAFAAKLAAAVATLKVGNGSEAGVTTGPL
                     IDARAVAKVQRHLDDALSQGARVLAGGQALGGNFFAPTVLVDVPPSALLAREETFGPL
                     APLLRFSDEADVIRQANATEFGLAAYFYARDLGRVFRVAEALEYGMVGINTGLISTEV
                     APFGGMKASGLGREGSKYGLDEYLEIKYLCLGGI"
     gene            5736..7016
                     /gene="gabT"
                     /locus_tag="VIO83_08285"
     CDS             5736..7016
                     /gene="gabT"
                     /locus_tag="VIO83_08285"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEY8331305.1"
                     /translation="MDSTNASLLQRRQAAVARGVSQVHPIVADHAHNATVVDVEGREY
                     LDFAGGIAVLNTGHLHPKVIAAVQAQLGKLTHTCFQVLAYEPYIALCEQIARRVPGDF
                     AKKTLLVTTGSEAVENAVKIARAATGRAGVIVFTGAYHGRTLMTLAMTGKKVPYAAGM
                     GLMPGGVYRAQFPCALHGVSVDDAIASIERVFKNDAEPRDIAAIVIEPVQGEGGFYPA
                     PVEFMQRLRALCDQHGILLVADEVQTGAGRTGTFFAMEQMGVLADLTTFAKSVGGGFP
                     IAGVCGKAEIMDVIAPGGLGGTYAGNPLACAAALAVLEVFEEEQLLERSQVIGERLRA
                     GLRALQAQHPSIVDVRGLGAMVALELGEGGNSHKPDAELTNRVVARARDKGLILLSCG
                     PYGNVLRMLVPLTVEDALLERGLAILGECFAELA"
     gene            complement(7068..>8001)
                     /locus_tag="VIO83_08290"
     CDS             complement(7068..>8001)
                     /locus_tag="VIO83_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HEY8331306.1"
                     /translation="DINLDIHSGELVALLGPSGCGKTTLLRIIAGLETPDAGSIAFHG
                     EDVSGHDVRDRNVGFVFQHYALFRHMTVFDNVAFGLRMKPKKERPGEAQIAEKVHELL
                     NMVQLDWLADRYPEQLSGGQRQRIALARALAVEPKILLLDEPFGALDAKVRKELRRWL
                     ARLHEEINLTSVFVTHDQEEAMEVADRIVVMNKGVIEQIGHPGEVYQRPASEFVYHFL
                     GDSNRLHLGEDRHVQFRPHEVVLAREAAADLEAAEVRDIRPLGAITRVTLRVDSQSEL
                     IEAEVTQDHPALAGLARGQVLYFKPQLSRSQVGA"
BASE COUNT         1390 a         2744 c         2650 g         1217 t
ORIGIN      
        1 ctgctggggc gccgtccccg ccagccgctc ggctttcagg tgggcatgga tctgtcgggc
       61 gccgcgggac gcgcctggag caacatggcg cattgtgtgt tcggcgagct gcaacgggcc
      121 ccccagctgt tcgaagcgcc gctgattcgt acccagttcg aacagacgct gatgaccacg
      181 ctgttgcaat ggcagccgaa caatctgcag ggcgaagggc ctgagcacag aaacaaggtg
      241 ctgccgcgct acgtcaagct ggccgaagag tacctgcagg cccatccgga gcagcccatc
      301 acggcggaga cgctggcgac cctcaccggc gtcagcgctc gctgtacagc gggttccaga
      361 gctttctcgg catgagtccc atgcgttacc tgcgggatat ccgcatggag cgggtacgcc
      421 aggatctgct cgacccgcac aagccgcgca gcgtcaccgc catagccacc cactggggct
      481 tcttccagct gggccgcttc gccaccgaat accgccacag gttcgccgaa gcgccccgcg
      541 agaccatcct caaaagacac taaaagtcgg caaatctggc gaatatgacg ctaagcacag
      601 gctaaatagc cacactcaaa ataactgcac tttgcgcata ctctctgcat tgcgcgcata
      661 gttgcccgcc gccccgttgc tccacattgg cattgccctc ccgcaggggg ctccaccaat
      721 agccgtcaac ggagcaacga agcatgcgca agatcaccat catcggagcc ggccaggccg
      781 gcctgcaact cggcatcggc ctgcttggcc agggctatgc cgtccgcatc gtctccaacc
      841 gcagccccga acagatcggc gcgggccgcg tcatgtccag ccaatccatg tacgacatgg
      901 cgctggcctt cgagcgagaa ctcgggatcg ccttctggga cgacgagtgc ccgcccaccg
      961 acggcgtgca catgcgcgca ggcagcggcg atcagttgct gatcgactgg cgcggcctga
     1021 tgggtgcacc ggggcaatcg gtggaccagc gcatcaagat gccggtgtgg atggccgagt
     1081 tccagcgtcg cggtggcgaa ctggtgatcg aggatgccgg catcccggag ctggagcgct
     1141 acgcgcagac cagcgacctg gtgatcgttt ccaccggcaa gggcgagatc ggcaagctgt
     1201 tcgagcgcga caacgacctg agcgcgttcg acgccccgct gcgcaccata tccctgacct
     1261 acgtgcacgg catgaagccg cgcgaggact tctccgccct caatatcagc atcaacccgg
     1321 gcatcggcga actggtcaac ttcccgggca tgaccctgtc cggcccctgc gacatcatca
     1381 acctcgagtg cattccgaat gggccgatgg accgctggca cgaggtgcag agccaggaag
     1441 aacatctggc gctggccgtg cagatgatcg aggagtactt cccctgggaa gccgagcgct
     1501 gccagaacct gcgcctgacc gacgaccagt gcacgctggt ggggcgcatc acgccgaccg
     1561 tgcgcaagcc ggtcggctac ctgccctccg gacgtaccgt gctcggcatg gccgatgtgc
     1621 tggtcctcaa cgatcccatc actggccagg gatccaacaa cgccagcaag tgcgccaacg
     1681 tgtacatgca atgcatcctc gaccagggcg agaaggcctt cgacgacgcg tggaagaatg
     1741 ccaccttcgc gcgctattgg gactacgccc aatgggtggc caagttcacc aacacccatc
     1801 tgctgccgcc taccgagccg gtactcaagg tgctcaccgc ctgtggccgc ctgccggagc
     1861 tggccgcgca cttctccaac gccttcaacg atccgaagat gctcaccccc tggtactacg
     1921 acagcgcgga agccgagcgc ttcatcgcct ccttcgacag caaagccgcc tgagggggaa
     1981 ccatgaccat aaaaaaacct aaacggcacg gcctgcgcat cgccgccagt accgttgccc
     2041 tgtgtgcgct tccctggagc atcgcccagg cgacccaggg caccatgccc cacggctacg
     2101 gggtgaaatc cgaaggcatg ggcggcgtgg tgatggcctt gccgcaggac gccctggtgg
     2161 gcgcatccaa cccggcgggc atggtgcagg taggcgatcg cctcgacctc ggcgtggcct
     2221 tcctcaaggt agaccagggc gccgagttcg ccggcacgga ctacgaggcc tcccacgacc
     2281 gggatctgta cgtgatcccg cagctcggcc tgaacaagat gctcgacgaa cgcagcagca
     2341 tcggcctcag cctggtcggc aatggcgtgg gtaccggcta cgccagctcg gacaatattg
     2401 gtggcctgca gagccccggc tcggaattcc agcagatggt cgggaccctg agctactcgc
     2461 gccaactgaa cgaggtgcac agcgtcggcg tggggctgct gctgatccgc caggcgctgg
     2521 atatcgacgg caccgccagc atcggcctgc ccgagggacg cgacgtggcc tatggcacgg
     2581 gcttgcgcct gggctggatg gcccgcctca acgacgaaat taccttcggc gccagctatg
     2641 ccacccgtgg ctacatgtcg aagatggacc gcttcgagaa tctgctcgcc gaagacggcg
     2701 acatggatat gccggaaacc tatggcatgg gcctggccta ccagaaggac cggctgacgc
     2761 ttggcctgga tgtgcaacgc atcatgtggg ccgacgtcaa atccctcgcc aacccgggcg
     2821 tcggttccgc aaccggcgcc cccggcagct ccaacggccc gggcttcggc tggcagaacc
     2881 agacggtatg gcgggtgggt gcggcctacg acgtactccc ggagctgacc ctgcgtgccg
     2941 gctacaacca cggcagcaaa ctcatcgaca gccaggacac ctacctcggc ctgctggcac
     3001 cgtccgccaa ccgccggcat gcgacggcgg gggcgaccta tcgcctcgat aacggccatg
     3061 aactgtccat ggcctacgcc cgctcgttca aggagaaggt caagggtgac ggcccgggac
     3121 cggacggtct gaccaacgtc tacatggggc aacactggct gtccctgagt tacggcatcg
     3181 cgttttaggg ggtggtatga acatgatcga agctaaacag ctacgccagg cattgagcaa
     3241 cttcgcgacc ggcatcacca tcgtgaccac ccaggatgat cggcagcaac cgatcggcgt
     3301 gaccgcgagc agcttcaact cggtgtcgct gaccccaccg ctggtgctgt ggagcatcgc
     3361 caaaagcgcc tacagctatg ccagcttcgc ccaggccgag cactttgccg tgcatgtgct
     3421 gggggcggat caggcagccc tgtccgaccg cttcgccaga gcctccaccg acaagttcgc
     3481 cgggctcgat accgccaggg gcgttggcga cgtgccgctg atacacggct gcatggcgcg
     3541 cttcgagtgt gcgaccgagc atcgctacga gggcggtgac cacatgatca tcgtcggccg
     3601 cgtgctgaac ctgaacctgt cggagcgccc gtgcgacccc ttgctgttct tcaagggacg
     3661 ttacgcgacc ctcgatggcg gggatcaggc ggcctaggcc ggcgggcgat ccttcctgcg
     3721 cgctgcgtgg gaaggatcat cgcgcagggg tcgacatcag gccggcccct gcaaaacctg
     3781 ggcaccgaga caacccgcaa cgccactgaa caacggcgat gcaggcagcg gcgatcagga
     3841 tcggcagggc tgcgcgtcga ttgaactgga gaacatttcg ggagggttct gagccgagga
     3901 ccggatcagg gagcgccttc aggaaaaagg cgctgaagcg aagtaaagat ggtgcaccag
     3961 gcgggattcg aacccacgac ccctgccttc ggagggcagt actctatcca gctgagctac
     4021 tggtgcagcg ggcgccatga tacgcatgtt ggcgggagtc gtccacggcg ccgaggggcg
     4081 ttcgcgatgt cgaacgcggc cacccgccag cgccgtcacc gccgctggcc aaccgccgtc
     4141 cacggtgcca tccgttgctt tttccgcaca gccattgccc gcaacgccag cccggcctag
     4201 gattgtttga catttcgaac atttctctgc ggagagcgca atggaactga aggaccccga
     4261 cctgttccgt cagcaggcct ttgtgaacgg ccagtggtgc gaggccgacc acggcgcgcg
     4321 caccgacatc cacaacccgg ccagcggcga aaagctgggc agcgtgccga acatgggccg
     4381 cgcggagacc cgccgcgcca tcgctgcggc gcaggcggcg cagccggcct ggcgcgcgct
     4441 gactgccaag gagcgttcaa ccaggttgcg caagtggtac gagctgatca tggccaacca
     4501 ggacgacctg gcgcagatca tgaccgccga gcagggcaag ccgctggccg aggcgcgcgg
     4561 cgaggtggcc tacggcgcct cgttcatcga gtggttcgcc gaggaggcca agcgcgccta
     4621 cggcgacgtg atcccggcgc atgccgccga caagcgcatc ctggtgcaga aggagccggt
     4681 cggcgtcacc gcggcgatca cgccgtggaa cttccccagc gcgatgatca cccgcaaggc
     4741 cggcccagcg ctggccgcgg gctgcgccat ggtgctcaag ccggcgccgc agacgccctt
     4801 ctccgccctg gccctggccg agctggcgca gcgcgccggg attcccgccg ggctgttcag
     4861 cgtgctgccg gccgacgtcg agaccagccg cgaagtcggc gccgagctgt gcgagaaccc
     4921 gatcgtgcgc aagctgtcgt tcaccggctc gaccgccgtc ggcatccagc tgatggcgca
     4981 gtgcgcgccg accctgaaga agctgtcgct ggagctgggc ggcaacgcgc ccttcatcgt
     5041 cttcgccgac gccgacctgg atgccgcggt cgagggggcg atgatctcca agtaccgcaa
     5101 cgccgggcag acctgcgtgt gcgccaaccg catctacgtg caggagtcgg tctacgacgc
     5161 cttcgccgcg aagctcgccg ccgcggtggc cacgctcaag gtgggcaacg gcagcgaggc
     5221 cggggtgacc accggcccgc tgatcgatgc ccgcgcggtg gccaaggtgc agcgccacct
     5281 cgacgacgcg ctgagccagg gcgcccgggt gctcgccggc ggccaggcgc tgggcggcaa
     5341 cttcttcgcg ccgaccgtgc tggtcgacgt gccgccgagc gcgctgctgg cccgcgagga
     5401 aaccttcggc ccgctggcgc cgctgctgcg tttcagcgac gaggctgacg tgatccgcca
     5461 ggccaacgcc accgaattcg gcctggccgc ctacttctac gcccgcgacc tgggccgggt
     5521 gttccgcgtc gccgaggcgc tggagtacgg catggtcggc atcaacaccg ggctgatctc
     5581 caccgaggtg gcgccgttcg gcggcatgaa ggcttccggc ctcggccgcg agggctccaa
     5641 gtacggcctc gacgagtacc tggagatcaa gtacctgtgc ctgggcggca tctgaaccac
     5701 tgctgcggcc gcatcgcggc catgcgagga agatcatgga cagcaccaac gcatccctgc
     5761 tgcaacgccg gcaggccgcc gtggcccgtg gcgtcagcca ggtccacccg atcgtcgccg
     5821 accacgcgca caacgccacg gtggtcgacg tcgagggccg cgagtacctc gacttcgccg
     5881 gcggcatcgc cgtgctcaac accggccacc tgcatcccaa ggtgatcgcc gcggtgcagg
     5941 cgcagctcgg caagctgacc cacacctgct tccaggtgct ggcctacgag ccgtacatcg
     6001 cgctgtgcga gcagatcgcc cggcgcgtgc cgggcgactt cgccaagaag accctgctgg
     6061 tcaccaccgg ctcggaggcg gtggaaaacg cggtgaagat cgcccgcgcc gccaccggcc
     6121 gcgccggggt gatcgtgttc accggcgcct accacggccg caccctgatg accctggcga
     6181 tgaccggcaa gaaggtgccc tacgccgccg gcatgggcct gatgccgggc ggcgtgtacc
     6241 gcgcccagtt tccctgcgcg ctgcacgggg tgagcgtcga cgacgccatc gccagcatcg
     6301 agcgggtgtt caagaacgac gccgagccgc gcgacatcgc cgccatcgtc atcgagccgg
     6361 tgcagggcga aggcggcttc tacccggcgc cggtggaatt catgcagcgc ctgcgcgcgc
     6421 tgtgcgacca gcacggcatc ctgctggtcg ccgacgaggt gcagaccggc gccgggcgca
     6481 ccggcacctt cttcgccatg gagcagatgg gcgtgctggc cgacctgacc accttcgcca
     6541 agtcggtggg cggcggcttc ccgatcgccg gggtgtgcgg caaggccgag atcatggacg
     6601 tgatcgcccc cggcggcctc ggcggcacct acgccggcaa cccgctggcc tgcgccgcgg
     6661 cgctggcggt gctggaggtg ttcgaggaag agcagttgct ggagcgcagc caggtcatcg
     6721 gcgaacggct gcgcgccgga ctgcgtgcgc tgcaggcgca acacccgagc atcgtcgacg
     6781 tgcgcggcct gggcgcgatg gtcgccctcg agttgggcga aggcggcaat tcgcacaagc
     6841 ccgacgccga gctgaccaac cgagtggtcg cccgcgcccg cgacaaggga ctgatcctgc
     6901 tgtcctgcgg cccctacggc aacgtgctgc gcatgctggt gccactgacc gtggaggacg
     6961 ccctgctcga gcgcggcctg gcgattctcg gcgagtgctt cgccgagctg gcctgagtca
     7021 cgccaccggc gcccggggct gcgcaacgcg ccccgggcgg cacggactca ggcgccgacc
     7081 tgcgagcggc tcagctgcgg cttgaagtac agcacctgac cgcgcgccag cccggccagt
     7141 gctggatggt cctgggtcac ctcggcctcg atcagctcgc tctggctgtc gacgcgcagg
     7201 gtgacccggg tgatggcgcc gagcgggcgg atgtcgcgca cctcggccgc ttccaggtcg
     7261 gccgccgcct cgcgcgccag caccacctcg tgcgggcgga actgcacgtg gcgatcctcg
     7321 cccaggtgca ggcggttgga gtcaccgagg aagtggtaga cgaactcgct ggccgggcgc
     7381 tggtagacct cgccgggatg gccgatctgc tcgatcaccc ccttgttcat caccacgatg
     7441 cggtcggcga cttccatcgc ctcttcctgg tcgtgggtga cgaacaccga ggtcaggttg
     7501 atctcctcgt gcaggcgcgc cagccagcgg cgcagctcct tgcgcacctt ggcatccagc
     7561 gcgccgaacg gctcgtcgag gaggaggatc ttcggctcca ccgccagcgc gcgggccagg
     7621 gcgatgcgct ggcgctggcc gccggacagc tgctccggat agcggtcggc cagccagtcg
     7681 agctgcacca tgttgagcag ttcgtggacc ttctcggcaa tctgcgcctc gccggggcgt
     7741 tccttcttcg gcttcatgcg caggccgaag gcgacgttgt cgaacaccgt catgtggcgg
     7801 aacagcgcgt agtgctggaa cacgaagccg acgttgcggt cgcgcacgtc gtggccggaa
     7861 acgtcctcgc cgtggaaggc gatgctgccg gcgtccgggg tttccaggcc ggcgatgatg
     7921 cgcagcaggg tggtcttgcc gcagccggac gggccgagca gggccaccag ctcgccgctg
     7981 tggatatcca ggttgatgtc g
//
LOCUS       DATCUN010000207         2499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_234276, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000207 DATCUN010000000
VERSION     DATCUN010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234276"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1937)
                     /gene="yccS"
                     /locus_tag="VIO83_08295"
     CDS             complement(<1..1937)
                     /gene="yccS"
                     /locus_tag="VIO83_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015637328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HEY8331307.1"
                     /translation="MPATSLHQSLRRLWALEKFSYSLRVFVALTGCMALSWTQDRMDL
                     LIPLFLGIIASALAETDDSWRGRLQALLVTLVCFAAAAFAVELLFPYPWLFVAALALS
                     TFALTMLGALGERYGTIASGTLILAIYTMITVDQRGGEVADLWQQPLLLVAGAAWYGL
                     LSVLWQALFSQQPVQQALARVFRELGLYLRLKAALFEPVRELDVEARRLELAQQNGKL
                     VAALNAAKEIILHRVGKGRATPQVNRYLKLYFVAQDIHERASSSHYPYNELAEAFFHS
                     DVLFRCRLLLRQQGRACRVLAEAIELRQPFAYQRASAQALDDLNASLAYLQAQQNPQW
                     RGLLRSLHALASNLDTLEQLLGAASNPDALAEAQDSTLFDRDPHSLREVGERLRQQLT
                     PTSLLFRHALRLALALAAGYGVLHWIHPEQGYWILLTTVFVCQPSYGATRRKLVQRIA
                     GTVLGLIAGWALFDLFPSAPVQSLIAVAAGVAFFATRSTRYTLATAAITLMVLFCFNQ
                     VGDGYGLIWPRLIDTLLGSLIAGLAVFLILPDWQGRRLPSVVANTLRCHSRYLREIML
                     QYASGKRDDLAYRLVRRNAHNADAALSAALANMLLEPGHFRREADTGFRCLALSHTLL
                     SYLSALGAHRAALPAELRGGL"
     gene            2109..>2499
                     /locus_tag="VIO83_08300"
     CDS             2109..>2499
                     /locus_tag="VIO83_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEY8331308.1"
                     /translation="MPPWLCRCCRGLLLGLLAPLWVLAAPAPVVVLTLDGAIGPASAD
                     YVVRGLARAEQQGAQLVVLTLDTPGGLDSSMRAIIKAILASPVPVASYVAPGGARAAS
                     AGTYILYASHVAAMAPGTNLGAATPVQI"
BASE COUNT          383 a          816 c          956 g          344 t
ORIGIN      
        1 atcagcccac cgcgcagctc ggccggcagc gccgcccggt gcgcgccgag ggccgacagg
       61 tagctgagca gggtgtgcga cagcgccagg cagcgaaagc cggtatccgc ctcgcggcgg
      121 aaatgtccgg gctccagcag catgttggcc agcgcggcgg acagcgcggc gtcggcgttg
      181 tgggcgttgc gccggaccag gcggtaggcc aggtcgtcgc gcttgccgct ggcgtactgc
      241 aacatgattt cccgcaggta gcggctgtgg cagcgcaggg tattggccac cacgctgggc
      301 aggcggcggc cctgccagtc gggcaggatc aggaacaccg cgaggccggc gatcaggctg
      361 ccgagcaggg tgtcgatcag ccgcggccag atcagcccgt agccgtcgcc gacctggttg
      421 aagcagaaca gcaccatcag ggtgatcgcc gcggtggcca gggtgtagcg cgtgctgcgg
      481 gtagcgaaga aggccacccc ggcggcgacg gcgatcagcg actgcacggg cgcgctgggg
      541 aacaggtcga acagcgccca gccggcgatc aggccgagta ccgtgccggc gatgcgctgt
      601 accagcttgc ggcgggtggc gccgtagctg ggctggcaga cgaacaccgt ggtcagcagg
      661 atccagtagc cctgctccgg atggatccag tgcagcacgc cgtagccggc ggccagcgcc
      721 agggccaggc gcagggcgtg gcggaacagc agcgaggtgg gggtgagctg ctggcgcagg
      781 cgctcgccga cctcgcgcag gctgtgcggg tcgcggtcga acagggtgct gtcctgggcc
      841 tcggcgaggg catccgggtt gctcgccgca ccgagcagtt gctcgagggt gtcgaggttg
      901 ctggccagcg cgtgcagcga gcgcagcagg ccgcgccact gcggattctg ctgcgcctgc
      961 aggtaggcca gcgaggcatt caggtcgtcg agggcctggg cgctggcgcg ctggtaggcg
     1021 aacggctggc gcagctcgat ggcttcggcc agcacgcggc aggcccggcc ctgctggcgc
     1081 agcagcagcc ggcagcggaa cagcacgtcg ctgtggaaga acgcctcggc cagctcgttg
     1141 taggggtagt gcgaggagct ggcgcgctcg tggatgtcct gggcgacgaa gtacagcttc
     1201 aggtagcgat tgacctgcgg cgtcgcccgg cccttgccga cccggtgcag gatgatctcc
     1261 ttggcggcgt tcagtgccgc caccagcttg ccgttctgct gcgccagctc caggcgccgc
     1321 gcttccacgt cgagctcgcg caccggctcg aacagcgccg ccttgaggcg caggtacagg
     1381 cccagctcgc ggaacacccg ggccagcgcc tgctgcactg gctgctggct gaacagcgcc
     1441 tgccagagca ccgagagcag gccgtaccag gccgcgccgg cgaccagcag caggggttgc
     1501 tgccacaggt cggcgacctc gccgccgcgc tggtcgacgg tgatcatggt gtagatggcc
     1561 aggatcaggg tgcccgaggc gatggtgccg tagcgctcgc ccagcgcacc gagcatggtc
     1621 agggcgaagg tggacagcgc cagcgcggcg acgaacagcc agggataggg gaacagcagc
     1681 tcgacggcga aggccgcggc ggcgaagcag accagggtca ccagcagcgc ctgcaggcga
     1741 ccgcgccagc tgtcgtcggt ctccgccagg gcgctggcga tgatgccgag gaacagcggg
     1801 atcagcaggt ccatgcggtc ctgggtccag ctcagggcca tgcagccggt gagggcgacg
     1861 aatacccgca ggctgtagct gaacttctcc agcgcccaca ggcggcgcag ggattggtgg
     1921 agcgaagtgg cgggcatggg gcgtcctgcg tgaaggtgca tgcggaggtc gcggcagcgg
     1981 caaccgtacg gctgcattct atacacagcc tccgcctggc ggtcagccgc tgcccgagcg
     2041 caaggacgtg gctggcctcg actatcctgc aaggaatcgc cttgcctgga cccgccggga
     2101 ggatggtcat gccgccatgg ctgtgtcgct gttgccgagg cctgctgctg gggctgctgg
     2161 caccgctgtg ggtgctggct gcgccggcgc cggtggtggt gctgacgctg gacggtgcca
     2221 tcggcccggc cagcgccgat tacgtggtcc gcggcctggc gcgtgccgaa cagcaaggcg
     2281 cgcagctggt ggtgctgacc ctcgataccc cgggcgggct ggacagctcg atgcgcgcga
     2341 tcatcaaggc gatcctcgcc agtcccgtgc cggtggccag ctatgtggcg cccggcggcg
     2401 cgcgggcggc cagcgccggc acctacatcc tgtatgccag ccatgtcgcc gccatggcgc
     2461 cgggcaccaa cctcggcgcc gccaccccgg tgcagatcg
//
LOCUS       DATCUN010000208         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_253885, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000208 DATCUN010000000
VERSION     DATCUN010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253885"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1024
                     /gene="yjiA"
                     /locus_tag="VIO83_08305"
     CDS             53..1024
                     /gene="yjiA"
                     /locus_tag="VIO83_08305"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY8331309.1"
                     /translation="MSHSPIPVTVLSGFLGAGKTTLLKHMLQAEHGLRLAVIENEYSE
                     TPIDGQLLGSEPVQLLTLANGCVCCSIHVELEKALYLLLDKLDSGELAFDRLVIECTG
                     LADPAPVAQTFFADEELCSRYVLDGILTLVDAANAGRHLQETIAQAQVGFADRILLSK
                     TDLVASAQVEALSQRLQRINRRAPIRVVEHGRIELAELLDVRGFNLNADFGPALSLRP
                     LLPAANKDRIATLVLKSERPLDMDRLSAFMEDLLERHGNALLRYKGVLHVAGEPRRMV
                     FQGVLRLYGFDWDAEWGMDEARESVLVFIGDNLPEEEIRAGFAALQV"
     gene            complement(1288..2544)
                     /gene="glyA"
                     /locus_tag="VIO83_08310"
     CDS             complement(1288..2544)
                     /gene="glyA"
                     /locus_tag="VIO83_08310"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY8331310.1"
                     /translation="MFSRDLTLARYDADLFAAMEQEAQRQEEHIELIASENYTSPAVM
                     EAQGSVLTNKYAEGYPHKRYYGGCEYVDIVEQLAIDRAKQLFGADYANVQPHAGSQAN
                     SAVFQALVKPGDTVLGMSLAHGGHLTHGASVNFSGKIYHAVQYGLNPETGLIDYDEVE
                     RLAVEHQPKMIIAGFSAYSQVLDFPRFREIADKVGAYLFVDMAHVAGLVAAGVYPNPV
                     PFADVVTTTTHKTLRGPRGGLILAKANEEIEKKLNSAVFPGGQGGPLMHVIAAKAVCF
                     KEALSPEFKAYQQQVVKNAQAMAEVFVERGFDVVSGGTQNHLFLLSLIKQDITGKDAD
                     AALGRAFITVNKNAVPNDPRSPFVTSGLRIGTPAVTTRGFGEAECRELAGWICDILQN
                     MGDESVIDAVREKVKAVCAKLPVYGK"
BASE COUNT          391 a          828 c          879 g          456 t
ORIGIN      
        1 tgaattcccg cgccacgccc cgtcgtggcg cgtttcgttt cgaggagcgc ccatgtcgca
       61 ttcccccatt ccggtcaccg tgctcagcgg ctttctcggt gccggcaaga ccaccctgct
      121 caagcacatg ctgcaggccg agcacggcct caggctggcg gtgatcgaga acgagtacag
      181 cgagacgccg atcgacggcc agttgctcgg cagcgagccg gtgcagctgc tgaccctggc
      241 caacggctgc gtctgctgct cgatccacgt cgagctggag aaggccctgt acctgctgct
      301 cgacaagctc gacagcggcg agctggcttt cgaccgcctg gtgatcgagt gcaccggcct
      361 ggccgacccg gcgccggtgg cgcagacctt cttcgccgac gaggagctgt gcagccgcta
      421 tgtgctggac ggcatcctca ccctggtcga cgcggccaac gccgggcgcc acctgcagga
      481 aaccatcgcc caggcgcagg tcggttttgc cgaccgcatc ctgctgagca agaccgatct
      541 ggtcgccagc gcgcaggtcg aggcgctgag ccagcgtctg cagcgcatca accgccgcgc
      601 gccaatccgc gtggtcgagc acggccgcat cgagctggcc gagctgctgg acgtgcgcgg
      661 cttcaacctc aacgccgact tcggtccggc gctgagcctg cgcccgctgc tgccggcggc
      721 gaacaaggat cgcatcgcca ccctggtgct caaaagcgag cgtccgctgg acatggaccg
      781 cctcagcgcc ttcatggagg acttgctgga gcgccacggc aacgcgctgc tgcgctacaa
      841 gggcgtgctg cacgtcgccg gcgagccgcg gcgcatggtg ttccagggcg tgctacgcct
      901 gtacggcttc gactgggacg cggagtgggg catggatgag gcgcgcgaga gcgtgctggt
      961 gttcatcggc gacaacctgc cggaggagga gatccgcgcg ggcttcgctg cgctgcaggt
     1021 gtagggtgaa caacggcgca gccttgtcca ccgtcctgcg gcagcggatg ccgggccggt
     1081 aactgctggt cgcgtaggct gggttggacc gcaggtcgta acccaccgac cggccgtgag
     1141 gccagcaatg ggatgcctgt cggcatcctt ggtgggttac gccgcttggc ggctaaccca
     1201 ccctacgagt ctcgcgccca caaaaaagcc cggcacttgg ccgggcttct tcgttgcagc
     1261 gctggcgctg ctttttaacg ctagagctta cttgccgtac accggcagct tggcgcagac
     1321 ggccttgacc ttctcgcgca cggcgtcgat caccgactcg tcgcccatgt tctgcaggat
     1381 gtcgcagatc cagccggcca gctcgcggca ctcggcttcg ccgaaaccgc gggtggtgac
     1441 ggccggggtg ccgatgcgca ggccggaggt gacgaacggc gaacgcgggt cgttcggcac
     1501 ggcgttcttg ttgacggtga tgaaggcgcg acccagggcg gcgtccgcat ccttgccggt
     1561 gatgtcctgc ttgatcagcg acagcaggaa caggtggttc tgggtaccgc cggaaaccac
     1621 gtcgaagccg cgctcgacga acacttcggc catggcctgg gcgttcttca ccacctgctg
     1681 ctggtaggcc ttgaactcgg ggctcagcgc ttccttgaag cacaccgcct tggcggcgat
     1741 cacgtgcatc agcgggccgc cctggccgcc cgggaacacg gcggagttca gcttcttctc
     1801 gatctcttcg ttggccttgg ccaggatcag gccgccgcgc gggccgcgca gggtcttgtg
     1861 ggtggtggtg gtgaccacgt cggcgaaagg taccgggttc gggtacacgc cggcggcgac
     1921 cagaccggcc acgtgggcca tgtcgacgaa caggtaggca ccgaccttgt cggcgatttc
     1981 gcggaagcgc gggaaatcca gcacctgcga gtaggcggag aagccggcga tgatcatctt
     2041 cggctggtgc tcgacggcca ggcgctcgac ctcgtcgtag tcgatcaggc cggtttccgg
     2101 gttcaggccg tactgcacgg cgtggtagat cttgccggag aagttgacgc tggcgccgtg
     2161 ggtcaggtga ccgccgtggg ccaggctcat gcccagcacg gtgtcgcccg gcttgaccag
     2221 cgcttggaac acggcgctgt tggcctggct accggcgtgc ggctggacgt tggcgtagtc
     2281 ggcgccgaac agctgcttgg cgcggtcgat ggccagctgc tcgacgatgt cgacgtactc
     2341 gcaaccaccg tagtagcgct tgtgcgggta gccttcggcg tacttgttgg tcagcacgct
     2401 gccctgggct tccatcaccg ccgggctggt gtagttctcc gaggcgatca gctcgatgtg
     2461 ctcttcctgg cgctgggctt cttgctccat ggcggcaaac aggtcggcgt cataacgggc
     2521 gagggtcaaa tcacggctga acatggcggt cctc
//
LOCUS       DATCUN010000209         2778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_103323, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000209 DATCUN010000000
VERSION     DATCUN010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103323"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..2079
                     /gene="recQ"
                     /locus_tag="VIO83_08315"
     CDS             277..2079
                     /gene="recQ"
                     /locus_tag="VIO83_08315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEY8331311.1"
                     /translation="MPSSPAHDLLSRIFGYDDFRGPQQAIVEHVAAGNDALVLMPTGG
                     GKSLCYQVPSLLREGTGIVISPLIALMQDQVEALRQLGVRAEFLNSTLDGDTAARVER
                     ELLAGELDMLYVAPERLLTGRFLSLLSRSRIALFAIDEAHCVSQWGHDFRPEYRQLTV
                     LHERWPDVPRIALTATADPPTQREIAERLDLTGAQHFVSSFDRPNIRYTVVQKDNAKR
                     QLLDFLKVHRQEAGIVYCMSRRKVEETAEFLCKEGLNALPYHAGLPAEVRAANQRRFL
                     REDGIVMCATIAFGMGIDKPDVRFVAHTDLPKSMEGYYQETGRAGRDGEPAEAWLCYG
                     LGDVVLLKQMIEQSEAGEERKSLERNKLDHLLGYCESMQCRRQVLLAGFGETYPQPCG
                     NCDNCLVPPDAWDASVAAQKALSCVYRSGQRFGVGHLIDILRGGENDKIRQFGHSELS
                     TYGIGKDLDARTWRSVFRQLVAASLLEVDTEAYGGLRLTDGSREVLKGQRKVMMRRES
                     PKTRERDRSGQRTGLSVLPEDLVLFNALRSLRAELAKEQNVPAFVIFHDSTLRNIAEQ
                     RPTSIDALGKVGGIGGTKLARYGERLVEIVREEG"
     gene            2174..2569
                     /locus_tag="VIO83_08320"
     CDS             2174..2569
                     /locus_tag="VIO83_08320"
                     /inference="COORDINATES: protein motif:HMM:NF033807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopL family metal-binding regulatory protein"
                     /protein_id="HEY8331312.1"
                     /translation="MTPGGLLLRVVLMLSLLLNGLNAAMAGPMTLAMAHATSAEPASP
                     CHGDAPAPAMPAHHDHAAADTAGTQAPHDGDHCKIKDCLRSCAQQPSLTVSVAWLPSP
                     PPPALAPLGHTRAALPMPPLDRITRPPIG"
BASE COUNT          449 a         1076 c          821 g          432 t
ORIGIN      
        1 ggggggacac gccgtaaatc catccctgga ggctcgtggg cgccatccat ggcgcccaac
       61 ggtcccgcca acccacccac cccgcgccaa ccatcggcag tgaaaccatg ggcttcggct
      121 tcggcttcgg ctctggctcc ggctctggct ttggctttgg ctttggcttt ggctttggct
      181 tgagattcgg cctggggagg ccagtgggca agcccctacc cactgatccg ctgttggagg
      241 acacggccgg cccgcgtggc ggggtaaact agtccgatgc cttccagccc cgcgcacgac
      301 ctgctcagcc gcatttttgg ttacgacgat ttccgcggtc cgcagcaggc gatcgttgaa
      361 catgtcgctg ccggcaatga cgccctcgta ttgatgccca ccggcggcgg caagtcgctg
      421 tgctaccagg tgccttccct cctccgcgag ggtaccggca tcgtcatctc cccgctcatt
      481 gccctcatgc aggaccaggt cgaagccctg cgccagctcg gcgtgcgcgc cgaattcctc
      541 aattccaccc tcgatggcga caccgccgcc cgggtcgaac gcgaactgct cgccggcgag
      601 ctcgacatgc tctatgtcgc ccccgagcgc ctgctcaccg gccgcttcct gtccctgctc
      661 tcgcgcagcc gcatcgccct gttcgccatc gacgaagccc actgcgtctc ccagtggggc
      721 cacgacttcc ggcccgagta ccgccagctt accgtcctgc atgaacgctg gcccgacgtc
      781 ccgcgcatcg ccctcaccgc caccgccgac ccgcctaccc agcgcgaaat cgccgaacgc
      841 ctcgacctca ccggcgccca gcacttcgtc agctccttcg accggcccaa catccgttac
      901 accgtcgtcc agaaagacaa cgccaagcgc cagctgctcg acttcctcaa agtccaccgc
      961 caggaagccg gcatcgtcta ctgcatgtcg cggcgcaagg tcgaggaaac cgccgaattc
     1021 ctgtgcaagg aaggcctcaa cgccctgccc taccacgccg gcctgcccgc tgaagtgcgc
     1081 gccgccaacc agcgccgctt cctgcgcgag gatggcatcg tcatgtgcgc caccatcgcc
     1141 ttcggcatgg gcatcgacaa acccgacgtc cgcttcgtcg cccacaccga cctgcccaag
     1201 tccatggaag ggtattacca ggaaaccggc cgtgccggcc gcgacggcga acccgccgaa
     1261 gcctggctct gctacggcct gggcgacgtg gtgctgctca agcagatgat cgaacagtcc
     1321 gaggccggcg aagaacgcaa aagcctcgaa cgcaacaagc tcgaccacct gctcggctac
     1381 tgcgaatcca tgcagtgccg gcgccaggtg ctgctggccg ggtttggcga aacctacccc
     1441 cagccctgcg gcaactgcga caactgcctg gtgccccccg acgcctggga cgccagcgtg
     1501 gccgcccaga aagcgctcag ctgcgtctac cgtagcggcc agcgcttcgg cgtgggccac
     1561 ctgatcgaca tcctgcgcgg cggcgagaac gacaagatcc gccagttcgg ccacagcgaa
     1621 ctgagcacct acggcatcgg caaggacctc gacgcccgca cctggcgcag cgtgttccgc
     1681 caactggtgg ccgccagcct gctcgaagtg gacaccgagg cctacggtgg cctgcgcctc
     1741 accgacggca gccgcgaggt cctcaagggc cagcgcaagg tcatgatgcg ccgcgaaagc
     1801 cccaagaccc gcgagcgcga ccgcagcggc cagcgtaccg gcctgtccgt gctgcccgaa
     1861 gacctggtcc tgttcaacgc gctgcgcagc ctgcgcgccg aactggccaa ggaacagaac
     1921 gtaccggcgt ttgtgatctt ccacgacagc accctgcgca acatcgccga acagcgccct
     1981 accagcatcg acgcgctcgg caaggtcggc ggcatcggcg gcaccaagct cgctcgctac
     2041 ggcgagcgcc tggtcgaaat cgtccgcgag gaaggctgat gcccacctgc gcccgcacgg
     2101 atacgcgtcc agagttgaac gccgcgttca tccacaggtg cgcacgcccc ggtttggcgc
     2161 tagggtgacg cccatgaccc ccggtggcct gctcctgcgc gtggtactga tgctcagcct
     2221 gttgctcaac gggctgaacg ccgccatggc cggcccgatg accctggcca tggcccacgc
     2281 caccagcgcc gagcccgcat cgccctgcca cggtgatgct ccggcccctg ccatgcccgc
     2341 ccatcacgac cacgccgctg ccgacaccgc cggcacgcag gcgccgcacg atggcgatca
     2401 ctgcaagatc aaggactgcc tgcgcagctg tgcgcagcag cccagcctga ccgtgtccgt
     2461 cgcgtggctg cccagcccgc cgccgcccgc gctggcgccc ctgggccaca cccgcgcggc
     2521 gttgccgatg ccgcccctgg accgcatcac ccgccctccg atcggctgag cctgcgctca
     2581 ctgcgagcgc ctgtgcgaag cgatgccgtg cgcccccgtg cgtgcgcatc ttgacggcac
     2641 ctgcgtgcct ttcgaatttg attcggagtt gcaatgtcct tttcccctcc ccccggcccg
     2701 ggcgccccac ccatgccgtc gcgccgtctg ttcgtgcagg gcgctgccgc cggtggcgtg
     2761 gtcgccggcc tggtggcc
//
LOCUS       DATCUN010000210         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_9159, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000210 DATCUN010000000
VERSION     DATCUN010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9159"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VIO83_08325"
     CDS             <1..372
                     /locus_tag="VIO83_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8331313.1"
                     /translation="DQAGGLDSRLSALGSMLGATLFLLPLFGYSVISQPPASWGGWSV
                     WLSLLGLGLVCTALAYIVYFRLLSSVGPVRSMTTTFLIPPFGVLWGALLLDEPLSMAH
                     VYGGVLIAAALWLVLKPARTR"
     gene            complement(482..2062)
                     /locus_tag="VIO83_08330"
     CDS             complement(482..2062)
                     /locus_tag="VIO83_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011059866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HEY8331314.1"
                     /translation="MTQFLGHNYIGGRRSANGTLQLQSLDASTGEPLPGTFFQASEAE
                     VDAAAKAAAAAYPIYRNLSAEKRATFLDAIADEIDALGEDFVATVCRETALPAGRIQG
                     ERGRTSGQLRLFARVLRRGDFYGARIDRALPERQPLPRPDLRQYRIALGPVAVFGASN
                     FPLAFSTAGGDTASALAAGCPVVFKAHSGHMATAEWVADAIIRAAERTQMPAGVFNMI
                     YGGGVGEWLVKHPAIQAVGFTGSLKGGNALSHMAATRPQPIPVFAEMSSINPVFLLPE
                     ALAVRGEQIAAQLAGSVTLGCGQFCTNPGLVIGLRSPQFSSFLEIFCASMNQQPAQTM
                     LNAAGLDSYSRGLGELHEHPGLTHLAGKPQQGNQAQPQVFKADVSLLLKGDELLQEEV
                     FGPTTIVIEVEDRAQLAAALQGLRGQLTATLIGEPDELLEYRWLAEMLQEKVGRILLN
                     GYPTGVEVCEAMVHGGPYPATSDSRGTSVGTLAIDRFLRPVCFQNYPDALLPEALQNA
                     NPLGIRRLVDGEVSSLAL"
BASE COUNT          368 a          662 c          666 g          393 t
ORIGIN      
        1 gatcaggccg gcggcctcga cagccgtttg tctgcgctgg gcagcatgct cggcgccacg
       61 ctgttcctgt tgccgttgtt cggctacagc gtgatcagcc aaccgccggc aagctggggc
      121 ggctggagtg tctggttgtc gctgctggga ttggggctgg tgtgcaccgc gcttgcgtac
      181 atcgtttact tccgcttgct cagttcggtc gggccggtga ggtcgatgac cacgaccttc
      241 ctgatcccgc cgttcggcgt gttatggggg gcgctgttac tggatgagcc attgtcgatg
      301 gcgcatgtct atggcggggt gttgattgcg gcggcgttgt ggctggtgtt gaagccggcc
      361 aggaccaggt aagttgctgc gacgatgatt gtaggagctg ccgcaggctg cgatcttttg
      421 atcttgtttt taaaagcaaa atcgaaagat cgcagcctgc ggcagctcct acagggggaa
      481 atcacaacgc caacgaactc acctccccat ccaccaatcg ccgaatcccc agcggattag
      541 cgttctgcaa cgcctcgggc agcaacgcgt ccggataatt ctggaagcac accgggcgca
      601 ggaaacggtc gatggccagg gtgccgaccg aggtcccgcg cgagtcggac gtcgccggat
      661 acggcccgcc atgcaccatc gcttcgcaca cctcgacgcc ggtcggatag ccattgagca
      721 gaatccgccc gaccttttcc tgcaacatct ccgccagcca gcgatactcc agcaactcgt
      781 caggctcgcc gatcaaggtc gccgtcaact gcccgcgcag gccctgtagc gccgccgcca
      841 actgcgcgcg gtcctcgact tcgatgacga tggtggtcgg cccgaatact tcttcctgaa
      901 gcaactcatc gcccttgagc aacaggctga catcggcctt gaacacttgc ggctgcgcct
      961 gattgccctg ttgcggcttg cccgccagat gagtcagccc cggatgttcg tgcagttcac
     1021 ccaaacctcg gctgtagctg tccagccctg cggcattgag catggtttgc gcaggttgct
     1081 ggttcatgct cgcgcaaaaa atttcgagaa agctgctgaa ctgcggcgaa cgcaagccga
     1141 tgaccaggcc tggattggta cagaactgac cacagcccag ggtcaccgag ccggcgagtt
     1201 gcgccgcgat ctgctcgccg cgcaccgcca gcgcctcggg cagcaggaac accgggttga
     1261 tgctcgacat ctcggcgaac accggaatcg gctgtggccg ggtcgcggcc atgtggctca
     1321 gggcattgcc acctttgagc gaaccggtga aacccacggc ctggatcgcc gggtgcttga
     1381 ccagccactc accgacgcca ccgccataaa tcatgttgaa aaccccggcc ggcatttgcg
     1441 tgcgttcggc ggcgcggatg atcgcgtccg ccacccattc ggcggttgcc atgtgcccgc
     1501 tgtgggcctt gaacaccacc gggcaaccgg cggccagggc cgaggcggta tcgccacccg
     1561 ccgtggagaa cgccaacgga aagttgctcg cgccgaacac ggccaccggc cccagggcga
     1621 tgcggtactg acgcagatcc gggcgcggca atggctggcg ctccggcagc gcccgatcga
     1681 tgcgtgcgcc gtagaaatca ccgcgacgca gcaccctggc gaacaagcgc aactggccgc
     1741 tggtgcgacc gcgttcgccc tggatacgcc cggccggcaa cgcggtttcg cggcaaacgg
     1801 tggcaacgaa atcctcgccc agcgcatcga tttcatcggc gatggcgtcg aggaaggtcg
     1861 cgcgtttttc cgcgctcagg ttgcggtaga tcgggtacgc cgccgcggcg gctttggcgg
     1921 cggcatccac ttcggcttcg gacgcctgaa agaaggtgcc gggcaatggc tcgccggtgc
     1981 ttgcatcgag actttgcagt tgcaaggtgc cgttggcgct gcggcggccg ccgatgtagt
     2041 tgtggccaag aaattgagtc atgtgagttc cttgatcaat ggcagacct
//
LOCUS       DATCUN010000211         5516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_28133, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000211 DATCUN010000000
VERSION     DATCUN010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28133"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /gene="recD"
                     /locus_tag="VIO83_08335"
     CDS             <1..1458
                     /gene="recD"
                     /locus_tag="VIO83_08335"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEY8331315.1"
                     /translation="SFSLITGGPGTGKTTTVVRLLALLQEPALAAGQPLRIRLAAPTG
                     KAAARLTESIGAQVASLPVKEDVRQAIPSEVTTLHRLLGSLPDSRHFRHHAGNPLPLD
                     VLVVDEASMIDLEMMACLLAALPRHGRLILLGDKDQLASVEAGALLGDLCRDAEGGFY
                     SPDTRAWLEAIGDETLADPALQQGDAARQPLAQRTVMLRHSRRFGSGSGIGRLARAVN
                     ARDPATARAILAEGSSDLHVLRLRGEQDAALERLLLDGLPAGEAKPQGYAHYLNVLRD
                     SRPAADCPPDDQRWQDWAGGVLKAFDDFQLLCAVRKGAWGVEGLNPRIAAALHKRGLL
                     ESDQGWYEGRPVLVTRNDYSLGLMNGDIGIALRLPEPAERPGEAPRPVLRVVFPRNDG
                     SGGLRFILPSRLSAVETVFAMTVHKSQGSEFAHTALILPDTLNPVLTKELVYTGITRA
                     RHWFSLVESRGGVFDEAVRRQVERRSGLVEALTNP"
     gene            1604..2050
                     /locus_tag="VIO83_08340"
     CDS             1604..2050
                     /locus_tag="VIO83_08340"
                     /inference="COORDINATES: protein motif:HMM:NF002007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEY8331316.1"
                     /translation="MSYMKWTSDLELGIEVIDNQHRRIVEYINDLHQAIDNQRRVEVG
                     EVLEQLVEYTMSHFAFEEDLQERAGYPFHHAHKKVHELFNRKIEDFQYRFDLGEDVSR
                     HLLTMLRAWLINHIKRDDADYAETVKIALATPQKQRGKGLFARLFG"
     gene            2600..4030
                     /locus_tag="VIO83_08345"
     CDS             2600..4030
                     /locus_tag="VIO83_08345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8331317.1"
                     /translation="MTLITQSKPASDTRSHTPRGAVLAAPGLLEQRYPDFEYFHDLDE
                     LLAFAGWDVLLLDCPAAQAGRYLFQLRRLEAYRFIPIYCCRDQDDWCRALGDGLPPAS
                     TDELIAQWRQWRERLALFNRGVPPERFDSRVLAWLWLRSQGEIRVLRDTSVAQHYRYP
                     LLEACAGTEEVNAAVWLQTMVQHGWLETGELVDRLRLCTGCGSGRLNYVDVCPECHAL
                     DIARQPSLHCFTCGNVGPQELFLKDGLLLCPNCLTRLRHIGSDYDRPLENYSCRSCQA
                     FFIDAAVEARCLDCGQVHAPDKLRVREVRHYRLAEAGRLRCRQGLQDSHADAAFSRLN
                     LRGGKEFAELLSWQIELVRRYGSPAFSLLGLRLVNLTGTLGALGEQRAHALVDSLVER
                     LVEMVRETDRCTRTSEDQLWLLLPHTGEEGLASLQQRLANSCAQLTELAAHGIEPRTV
                     GFTAPQDLLDQEDGRLLMARLAGELL"
     gene            4034..5362
                     /locus_tag="VIO83_08350"
     CDS             4034..5362
                     /locus_tag="VIO83_08350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8331318.1"
                     /translation="MDDIAAIVLGLYAEVARDGGGTALLIALFPAFLLLEVPLNLLIL
                     LGVLRWFTRQRFVRPCSGSYRPRVSCIITCYSEGRDVRKTLESLCEQVYPGDIEMIPV
                     VDGAAVNHETLRAIREFRVDARRYPRRLLRPIAKWQRGGRVSSLNAGLAYASGEVVMA
                     LDGDTSFDNNMVGAMVRHFADPCVPAVAGSLRVRNASSSWITAVQALEYLLSIHLAKI
                     GLGEWNLVNNISGAFGAFRRSVLDKVGGWDTHTAEDLDITLRIKNYFGRDSLRIPFEP
                     EAIGHTDAPVTLRQLLMQRLRWDGDLYFLYVRKHRHSLTPRLLGWPNFLMTLLTGFFF
                     QLVLPFIILGYCLVGLLVMPWPALLALAGLIYLVYLGSTLLLYLVLLLAVSERPRQDL
                     RLAPVVLIFPLCMLGMRCWSAVATLNEMLRRGHEETSMAPWWVLKKAKRF"
BASE COUNT          828 a         1859 c         1845 g          984 t
ORIGIN      
        1 agcttcagcc tgatcaccgg cggccccggc accggcaaga ccaccacggt ggtgcgcctg
       61 ctggcgctgc tgcaggagcc ggcactggcc gccggtcagc cgctgcgtat ccgtctggcc
      121 gcgcccaccg gcaaggccgc cgcgcggctg accgagtcca tcggcgccca ggtcgcctcg
      181 ctgccggtca aggaggacgt gcgccaggcg atccccagcg aagtcaccac cttgcaccgc
      241 ctgctcggca gcctgccgga cagccgccac ttccgccacc acgccggcaa cccgctgccg
      301 ttggacgtgc tggtggtcga cgaggcgtcg atgatcgacc tggagatgat ggcctgcctg
      361 ctcgccgcct tgccgcgcca cggccggctg atcctgctcg gcgacaagga ccagctggcc
      421 tcggtggagg ccggcgcgct gctcggcgac ctgtgccgcg atgccgaggg cggtttctac
      481 agcccggaca cccgtgcctg gctggaggcg atcggcgacg aaaccctcgc cgatccggcg
      541 ctgcagcaag gcgacgcggc gcgccagcca ttggcccaac gcaccgtgat gctgcgccac
      601 tcgcggcgct tcggcagcgg ctcggggatc ggccgtctgg cccgtgcggt caacgcccgc
      661 gacccggcca cggcacgggc gatcctcgcc gagggcagca gcgatctgca cgtgctgcgt
      721 ctgcgcggcg aacaggacgc agcgctggag cgcctgctgc tcgacggcct gcccgctggc
      781 gaggccaagc cgcagggcta tgcccactac ctgaacgtgc tgcgtgactc gcgtcccgcc
      841 gccgattgcc cgccagacga tcagcgttgg caggactggg ccggtggcgt gctcaaggcc
      901 ttcgacgact tccagctgct ctgcgccgtg cgcaagggcg cctggggcgt ggaagggctc
      961 aacccgcgca tcgctgccgc cctgcacaag cgcggcctgc tggagagcga ccagggctgg
     1021 tacgagggcc gtccggtgct ggtgacgcgc aacgactaca gcctgggcct gatgaacggc
     1081 gacatcggca tcgccctgcg cctgcccgag ccggccgagc gtccgggcga ggcgccgcgc
     1141 ccggtgctgc gcgtggtgtt cccgcgcaac gatggctcgg gcggcctgcg cttcatcctg
     1201 cccagccggc tcagcgcggt ggaaacggtg ttcgccatga ccgtgcacaa gtcgcagggc
     1261 tcggagttcg cccatacggc cttgatcctg cccgacaccc tcaacccggt gctgaccaag
     1321 gaactggtct acaccggcat cacccgcgcg cgacactggt tcagcctggt cgagagccgc
     1381 ggcggcgtgt tcgacgaggc ggtgcgccgc caggtggagc ggcgcagcgg gctggtggag
     1441 gcgctgacca atccctgagg gcgccgggat tccgccccgg gagggcaggg gcggaattcg
     1501 tagtattgca tgtggcggtt ttctggcttt ttgtgctggt tccggtcttg gtgtgatctg
     1561 ggtgtcagaa tatcgtgcat caccatgcac catggacggc cctatgagct acatgaaatg
     1621 gaccagcgat ctggaactcg gcatcgaggt catcgacaat cagcaccggc gcatcgtcga
     1681 gtacatcaac gatctgcacc aggccatcga caaccagagg cgggtggaag tcggcgaagt
     1741 gctggagcaa ctggtggagt acaccatgtc gcacttcgcc ttcgaggagg atctgcagga
     1801 gcgcgccggc tatcccttcc atcatgcgca caagaaggtc cacgaactgt tcaaccgcaa
     1861 gatcgaggac ttccagtacc gcttcgacct gggcgaggat gtttcccgcc acctgctcac
     1921 catgctgcgc gcctggctga tcaaccacat caagcgcgat gatgccgact acgccgaaac
     1981 ggtgaagatc gccctggcca cgccgcagaa acagcgcggc aagggcctgt tcgcccgcct
     2041 gtttggctga gcgcagtgtt cccgacgtcg ctcgatgagt gactgcaggg gcgcctgcag
     2101 atgcgccgcg gcggcacggc cactgccgct gccgcccgga tcaatcgctt ccgagggcct
     2161 gcgcggccga ggatgggcgt cggcctgcgg gtgctcgatg attttcgcgg gattctggcg
     2221 ctgtatggat ttctgtattt gtgtggcgag tatttagaca aaaaaatgac gttatttgtt
     2281 gctgggaagg caccgcgacc tgatcggatc ggtataatcc ggcgccgaac aaccgcttgc
     2341 gggtgttatc tgtttgcgga catgcgggcc gaccgtggca ttagtggcct gtttggttag
     2401 ttcagttagt caatttcggc gcccatggcc ccgatacggc gttgccactc ctcgccatag
     2461 ccctgctatg actcgtcgcg gcaccttgtc tcggaaccat ggtcatccga acttgagttg
     2521 accaaccgca caggccacca ggttgctggc ggtggggcac tttccggtcg atgaataatt
     2581 actcccagcc gagcctccct tgacactcat cactcaatcg aagcccgcct ccgatacgcg
     2641 cagccacacc cctcgcggtg ccgtactggc cgcgcccggt ctgctcgagc agcgctatcc
     2701 cgacttcgaa tatttccacg acctggacga gttgctggcc tttgccggct gggacgtgct
     2761 cctgctcgac tgcccggctg cccaggccgg tcgctacctc ttccagctgc gccgcctcga
     2821 agcctatcgc ttcattccga tctactgctg ccgcgatcag gatgactggt gccgggcgct
     2881 gggcgatggc ctgccgccgg ccagcacgga tgaactgatt gcgcagtggc gacagtggcg
     2941 agagcgcctg gccctgttca accgcggtgt gccgccggag cgcttcgaca gccgggtact
     3001 ggcctggctg tggttgcgct cgcagggcga gatccgcgtg ctgcgcgaca cctcggtggc
     3061 gcagcactat cgctacccgt tgctggaggc ctgtgctggg acggaggagg tcaatgccgc
     3121 cgtctggttg cagacgatgg tccagcacgg ctggctggag accggcgaac tggtcgatcg
     3181 cctgcgcctg tgcaccggct gcggttcggg gcggctcaac tacgtggacg tctgccccga
     3241 gtgtcacgcg ctggacattg cccgccagcc gtcactgcac tgcttcacct gcggcaacgt
     3301 cggcccgcag gagctgttcc tcaaggacgg gctgctgctg tgccccaact gcctgacccg
     3361 cctgcgccac atcggcagcg actacgaccg tccgctggag aactacagct gccgcagctg
     3421 ccaggcgttc ttcatcgacg cggccgtcga ggcgcgctgc ctggattgcg gccaggtgca
     3481 cgcgccggac aagctgcggg tccgcgaggt gcgccactac cgcctggccg aggcggggcg
     3541 cctgcgctgt cgccagggct tgcaggacag ccacgccgac gcggcctttt cgcggctcaa
     3601 cctgcgcggc ggcaaggagt tcgccgagct gctcagctgg cagatcgagc tggtgcgccg
     3661 ctatggcagc cctgccttct cgctgctcgg gctgcgcctg gtcaatctga ccgggaccct
     3721 cggcgcgctg ggcgaacagc gagcccatgc gctggtcgac agcctggtcg agcgcctggt
     3781 ggagatggtg cgcgagaccg atcgctgcac ccgcaccagc gaggatcagc tctggttgct
     3841 gctgccgcat accggcgagg agggactggc cagtctccag cagcgtctgg cgaactcctg
     3901 cgcgcagctc acggagctcg ccgctcacgg gatcgagccg cgcaccgtgg gtttcactgc
     3961 gccgcaggat ctgctcgatc aggaagacgg gcgactgctg atggcgcgcc tggcggggga
     4021 actgctctga gtcatggatg acatcgcggc catagtcctc ggcctgtatg ccgaggttgc
     4081 cagggacggt ggcgggacgg cactgctgat tgccctgttc ccggccttcc tgctgctcga
     4141 agtgcccctc aacctgctga tcctgctggg cgtgctgcgc tggttcaccc gccagcgctt
     4201 cgtccgcccc tgctcgggca gctaccgccc gcgggtctcc tgcatcatca cctgctacag
     4261 cgagggtcgc gacgtgcgca agaccctgga aagcctgtgc gagcaggtct atccgggcga
     4321 catcgagatg atcccggtgg tcgatggtgc cgcggtcaat cacgaaaccc tgcgcgcgat
     4381 ccgtgagttt cgcgtcgatg cccggcgcta tccgcggcgc ctgctgcggc cgatcgccaa
     4441 gtggcagcgc ggtggccggg tgtcctcgct gaatgccggg ctggcctatg ccagcgggga
     4501 ggttgtcatg gccctggacg gcgacacctc gttcgacaac aacatggtcg gcgccatggt
     4561 gcgccacttc gccgacccgt gcgtgccggc ggtggccggc agcctgcggg tacgcaacgc
     4621 cagcagctcc tggatcacgg ccgtgcaggc gctggagtac ctgctgtcca tccacctggc
     4681 caagatcggc ctcggcgaat ggaacctggt gaacaatatt tccggggcct tcggcgcctt
     4741 ccgccgcagc gtgctcgaca aggtcggtgg ctgggatacc catacggcgg aagacctcga
     4801 catcaccctg cggatcaaga actacttcgg ccgcgatagc ctgcgtattc ccttcgagcc
     4861 ggaggcgatc ggccataccg acgcgccggt gactctgcgc cagttgctca tgcagcgcct
     4921 gcgctgggat ggcgacctgt acttcctcta cgtgcgcaag catcgccaca gcctgacgcc
     4981 gcgcctgctg ggctggccga acttcctgat gaccctgctc accggcttct tcttccagct
     5041 ggtgctgccg ttcatcatcc ttggctattg cctggtcggt ctgctggtga tgccctggcc
     5101 ggcgctgctg gcgctggctg gcctgatcta tctggtgtac ctcggcagca ccctgctgct
     5161 gtacctggtg ctgctgctgg ccgtctccga acggccgcgg caggatctgc gcctggctcc
     5221 ggtggtgctg atcttcccgc tgtgcatgtt gggcatgcgc tgctggagcg cggtggccac
     5281 gctcaacgag atgctgcgtc gtggtcacga ggaaaccagc atggcgccct ggtgggtact
     5341 gaaaaaggcc aaaaggttct gaatgactcg actgacgctg ttgctgcttg ccgcctggct
     5401 gccggtgctg cccctgcagg ccgagcctct gtcgctggtg cgcctgctgg agcaggcggc
     5461 cccggcgccg acgcagcgcc agggcgccgc cgagttggag gcgatgcagg cggcga
//
LOCUS       DATCUN010000212         7395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_197035, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000212 DATCUN010000000
VERSION     DATCUN010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197035"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..2029)
                     /locus_tag="VIO83_08355"
     CDS             complement(281..2029)
                     /locus_tag="VIO83_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003142154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY8331319.1"
                     /translation="MLAIAFIQDLAVLMLAAGLVTVLCHRLQQPVVLGYIVAGFLIGP
                     HTPPVALISDEQSIRTLAELGVIFLMFGLGLEFRLRRLLRVGPTAILAAFFEIVLMIW
                     LGYQLGRLFAWSHMDALFLGAILAISSTTIIVKALGDLKMKHERFAQLIFGILIVEDV
                     LGIGLVALLSGIAASGTVATAEVLATLGKLLLFMLVSLLLGLLLVPRILAYVARFGSD
                     ETLLITVLGLCFGFCLLVIKLGYSMVLGAFLIGAIMAESRQLAQIERLVEPLRHMFSA
                     IFFVAIGLLIDPAVLRDYAWPIAAISLAVVLGKLLTCGIGAFVTGNDGRTSLRVGMGL
                     AQIGEFSFIIAALGVKLEVTSDFLYPVAVAVSAITTLTTPYLIRAADPLARQLAEAMP
                     AHVAGVFGAYGTWLRNIRQPGQHALLAALIRRSLLHAVINLALVAAIFLGGGYFAAPI
                     DLWLDQWLPQPNLHKALIWGAALLLSLPLLLAAYHRLKALSLLLAELGGHSSRSRRVR
                     RALSELIPLLTLGSFLPLLAALSSSILPPREWLLIIGLLAAVLLALLWRWFVRVHRQL
                     HSALQETLDQDEPPES"
     gene            2248..2739
                     /gene="purE"
                     /locus_tag="VIO83_08360"
     CDS             2248..2739
                     /gene="purE"
                     /locus_tag="VIO83_08360"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY8331320.1"
                     /translation="MSALVGVIMGSKSDWSTLSHTVDMLDKLGIPHEVKVVSAHRTPD
                     LLFQYAEQAEERGLEVIIAGAGGAAHLPGMCAAKTHLPVLGVPVQSSMLSGVDSLLSI
                     VQMPGGIPVATLAIGKAGAINAALLAASILGGKHPQFHAALKEFRAQQTESVLENPDP
                     REI"
     gene            2751..3833
                     /locus_tag="VIO83_08365"
     CDS             2751..3833
                     /locus_tag="VIO83_08365"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY8331321.1"
                     /translation="MKIGVIGGGQLGRMLALAGTPLGMNFAFLDPAPDACAAALGEHL
                     RADYDDKDHLRRLADEVDLVTFEFESVPAETVAYLAHFVPVYPSDESLRIARDRWFEK
                     SMFKDLGIPTPEFADIHSQADLDAAAAAIGLPAVLKTRTLGYDGKGQKVLRKPEDVAG
                     AFAELGSVPCILEGFVPFTGEVSLVAVRGRDGETRFYPLVHNTHVDGILRLSIASQAH
                     PLQALAEDYVGRVLEKLDYVGVLAFEFFEVDGGLKANEIAPRVHNSGHWTIEGSECSQ
                     FENHLRAIAGLPLGSTAKVGESAMLNFIGEVPAVDQVIAIDDCHLHHYGKAFKVGRKV
                     GHATLRSADLPTLKAQIAKVEALIAG"
     gene            4053..4298
                     /locus_tag="VIO83_08370"
     CDS             4053..4298
                     /locus_tag="VIO83_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY8331322.1"
                     /translation="MGILATLLIGLLVGVLARWLKPGRHPMGWIMTILLGIGGSVLAT
                     YGGQALGIYRAGQAAGFVGAVVGAIVLLIVFGYLKKS"
     gene            4337..4858
                     /locus_tag="VIO83_08375"
     CDS             4337..4858
                     /locus_tag="VIO83_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015272353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HEY8331323.1"
                     /translation="MRLPFVVCLSLIVPLSHAEPEEVDWLDLLTAEDLDAMLAMPEIG
                     HDGGEGDSTFYTPGGLKQLPNLPAVMYSTRTVAALDEQALSLVGYPVPLEREAGSPLR
                     RFLLLPYPGACNHLPPPPPNQIVLVSYPAGIAVDDPYVPLRVSGTLKVQAQGDAAYTL
                     DADSVGAVEEIEL"
     gene            complement(4889..5809)
                     /gene="pbpG"
                     /locus_tag="VIO83_08380"
     CDS             complement(4889..5809)
                     /gene="pbpG"
                     /locus_tag="VIO83_08380"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEY8331324.1"
                     /translation="MRTQIRSSLFTLLLSCAWIALTPTAHAAGPAAAHDPARLQLASS
                     SAMVVDLDSDQVLFADKPDSVRPIASISKLMTAVVTLDAQLPMDEVIPVTIHDTKELR
                     GVYSRVRVNSQISRKEMLRLALMSSENRAASALAHNYPGGHARFVAAMNAKAKALGMN
                     RTRFVEPTGLSEFNVSTARDLVRLLKAARQYPMIRELSTTPSKTASFRKPNYVLGFNN
                     TNPLVRNPGWDIEVTKTGFINEAGHCLALLTRVANRPVAMVLLDTSGKRSHIGDATRV
                     RRWLETGKGSPVPAGAKSYRKTVASGSHIE"
     gene            5975..7369
                     /locus_tag="VIO83_08385"
     CDS             5975..7369
                     /locus_tag="VIO83_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010792435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY8331325.1"
                     /translation="MSSSGIAEHAVEALSARERERFIAANPQSVALAERARQSLFGGV
                     PMHWMSDWSTPAPLFVESAQGARFLDVDGHQYLDFCLGDTGSMFGHSPAPIAAALREQ
                     AGRGLTTMLPGEDAVACGELLAARFGLPFWQVVTTATDANRFVLRWARAITGRKVILV
                     FDGCYHGTVDETLVRLRDGHTVHRPGLIGQARNLTQHSRVVPFNDLAALEAALAPGDV
                     AAVLCEPALTNIGMVLPQPGFMARLRELTRQAGSLLIVDETHTLSTGPGGCTRAWQLE
                     PDFLTLGKAIAGGVPCAVYGCSAEMAQAMQLTRAHSREMAEGHGHSGMGTTLSANALA
                     MHCLRANLEQVMTAPAYEHMLWLAARLAEGFRALIARHALPWSVTELGARCEFQFRAT
                     PPQNGAEAEAAFHDSLQMALHLYLINRGILITPFHNMTLCCPETSAGDVDQLLAALDA
                     ALSELLAMPGARQA"
BASE COUNT         1200 a         2591 c         2536 g         1068 t
ORIGIN      
        1 ggccggcctc ccgcgcgccg accagcgcgg cttcgtccag cccggccatc ggccgcagcc
       61 aggcaatgcg gccgtcctcg accagcacgg ccatcggctc gggcagttcg caccggccgt
      121 cgaacacgcg aacatcgcgc cacagcaggc gttgcgggga acctgcggac atcggacgat
      181 ctccctgttg cgagccgggc gcagcggtag gagcaccgcc gcgggcagct caggccaatc
      241 actctctcta aggtataggc gctgatcggc ccggcgtgcc tcaggactcg ggcggctcat
      301 cctggtcgag ggtttcctgc agggcgctgt gcagctggcg atgcacgcgg acgaaccagc
      361 gccagagcag ggcgagcagc acggcagcga gcaggccgat gatcagcagc cattcgcgcg
      421 gcggcaggat gctcgagctg agcgcggcga gcagcggcag gaagctgccg agggtcagca
      481 gcggaatcag ctcggacagc gcccggcgca cgcggcggct gcggctgctg tgcccgccca
      541 gctcggcgag cagcagcgac agggccttga gccggtgata ggcggccagc agcagcggca
      601 gcgacagcag cagcgccgca ccccagatca gcgccttgtg caggttgggc tgcggcagcc
      661 actggtcgag ccagaggtcg atcggcgcgg cgaaatagcc gccgccgagg aagatcgccg
      721 ccaccagcgc caagttgatg accgcgtgca gcaggctgcg ccggatcagc gccgccagca
      781 gggcatgctg acccggctgg cggatgttgc gcagccaggt gccgtaggcg ccgaacactc
      841 ccgccacatg cgccggcatg gcctcggcca gctgccgcgc cagcggatcg gcggcgcgga
      901 tcaggtaggg agtggtcagg gtggtgatcg ccgagaccgc caccgccacc gggtagagga
      961 agtcgctggt gacctcgagc ttgaccccca gcgcggcgat gatgaaggag aactcgccga
     1021 tctgcgccag gcccatgccg acgcgcagcg aagtgcgccc gtcgttgccg gtgacgaagg
     1081 cgccgatgcc gcaggtgagc agcttgccga gcaccacggc gaggctgatc gccgcgatcg
     1141 gccaggcgta gtcgcgcagc accgccgggt cgatcagcag gccgatggcg acgaagaaga
     1201 tcgcgctgaa catgtggcgc agcggctcga ccagccgctc gatctgcgcc agctggcgcg
     1261 actcggccat gatcgcgccg atcaggaagg cgcccagcac catgctgtag cccagcttga
     1321 tcaccagcag gcagaagccg aagcacaggc cgagcacggt gatcagcagc gtctcgtcgc
     1381 tgccgaagcg ggccacgtag gcgaggatgc gcggcaccag cagcaggccg agcagcagcg
     1441 acaccagcat gaacagcagc agcttgccga gggtggcgag cacctcggcg gtcgccaccg
     1501 tgccgctggc ggcgatgccg gagagcaggg cgacgaggcc gatgccgagc acgtcctcga
     1561 cgatcaggat gccgaagatc agctgggcga agcgctcgtg cttcatcttc aggtcgccga
     1621 gcgccttgac gatgatggtg gtcgaggaga tcgccaggat cgcgccgaga aacagcgcat
     1681 ccatgtggct ccaggcgaac aggcggccga gctggtagcc gagccagatc atcagcacga
     1741 tctcgaagaa ggcggcgagg atcgcggtgg gaccgacgcg cagcagccgg cgcaggcgga
     1801 actcgagacc gaggccgaac atcaggaaga tcacccccag ctcggcgagg gtgcggatgc
     1861 tctgctcgtc actgatcagc gccaccggcg gggtgtgcgg gccgatcagg aagccggcga
     1921 cgatgtagcc gagcaccacc ggctgctgga ggcggtggca cagcaccgtc accagcccgg
     1981 ccgccagcat gagcacggca agatcctgga tgaaggcgat ggcaagcatg gcgcacactc
     2041 ccggggcggg gccgggcaac ggcgcgcatc cccagttcga tgataggcgg gtctgcccgt
     2101 cgcgcgaacc tttgcgatat actgcgcgac tttcgaccgg tcacgcagtc cggtccacac
     2161 tccttcgcag acaagccacg tgcggttatc cggcgtggct tgttggtttt tgtcgcgcca
     2221 ggttggcgca gaacaagagg cacgacgatg agcgcactgg taggcgtgat catgggctcc
     2281 aaatccgact ggagcaccct cagccacaca gtcgacatgc tggacaagct cggcatcccc
     2341 cacgaggtca aggtggtctc cgcccaccgc accccggacc tgctgttcca gtacgccgaa
     2401 caggcggaag agcgcggcct ggaagtgatc atcgccggtg ccggcggcgc cgcccacctg
     2461 ccgggcatgt gcgcagccaa gacccacctg ccggtgctcg gcgtgccggt gcagtcgtcg
     2521 atgctgtccg gcgtcgactc gctgctgtcc atcgtgcaga tgcccggcgg catcccggtc
     2581 gccaccctgg ccatcggcaa ggccggcgcg atcaatgccg cgctgctggc ggcgagcatc
     2641 ctcggcggca agcatccgca gttccacgcg gcactcaagg agttccgcgc ccagcagacc
     2701 gaatcggtgc tggaaaaccc ggacccccgc gaaatctgag gcgaacactc atgaagatcg
     2761 gcgtaatcgg tggcggccag ctgggtcgca tgctggccct ggcgggcact ccgctgggga
     2821 tgaactttgc cttcctcgac ccggcgccgg acgcctgcgc cgccgccctc ggcgagcacc
     2881 tgcgcgccga ctacgacgac aaggaccacc tgcgccgcct ggccgacgag gtggatctgg
     2941 tcaccttcga gttcgagagc gtgccggccg agaccgtggc ctacctcgcg catttcgtgc
     3001 cggtgtatcc gagcgacgaa tcgctgcgca tcgcccgcga ccgctggttc gagaagtcga
     3061 tgttcaagga cctcggcatc cccaccccgg aattcgccga catccactcg caggccgacc
     3121 tggacgccgc ggccgccgcc atcggcctgc cggcggtgct gaagacccgt accctcggct
     3181 acgacggcaa gggccagaag gtcctgcgca agcccgagga cgtcgccggt gccttcgccg
     3241 agctgggcag cgtgccgtgc atcctcgaag gcttcgtgcc cttcaccggc gaagtgtcgc
     3301 tggtggcggt gcgcggccgc gacggcgaga cgcgcttcta cccgctggtg cacaacaccc
     3361 acgtcgacgg catcctgcgc ctgtccatcg ccagccaggc gcacccgctg caggcgctgg
     3421 ccgaggacta cgtcggccgc gtgctggaga agctcgacta cgtcggcgtg ctggccttcg
     3481 agttcttcga ggtcgacggc ggcctcaagg ccaacgagat cgccccgcgc gtgcacaact
     3541 ccgggcactg gaccatcgag ggctccgagt gcagccagtt cgagaaccac ctgcgcgcca
     3601 tcgccggcct gccgctgggc tcgaccgcca aggtcggcga gagcgccatg ctcaacttca
     3661 tcggcgaagt gccggcggtg gaccaggtga tcgccatcga cgactgccac ctgcaccact
     3721 acggcaaggc cttcaaggtc ggccgcaagg tcggccacgc caccctgcgc agcgccgatc
     3781 tgccgaccct caaggcgcag atcgccaagg tcgaggcgct gatcgcgggc tgatcgcccg
     3841 cgtcccgtag ggtgggttag ccgcaaagcg gcgtaaccca ccaacgatgc cagccggcat
     3901 cgccatcgcc ggcctttgca ggccgggtgg tgggttgcgg cctgcggcct gacccaccct
     3961 acgattcccc gtcctggcga tgatcgcacg ggcaggcagc gctcccgccg actaggctag
     4021 aaggcaagtc catccctacg ggaacacttg ccatgggcat cctcgccacc ctcctcatcg
     4081 gcctgctggt cggcgtgctc gcccgctggc tcaagccggg ccgccatccg atgggctgga
     4141 tcatgaccat cctgctcggc atcggcggct cggttctcgc cacctacggc ggccaggcgc
     4201 tgggcatcta ccgcgccggc caggcggccg gcttcgttgg cgcggtggtc ggcgccatcg
     4261 ttctgctgat agtgttcggc tacctgaaga aatcctgaac cggcgccacg gcgccggtct
     4321 tcgccgcgag atccgcatgc gcctgccgtt cgtcgtctgc ctgagcctga tcgtccccct
     4381 gagccacgcc gagcccgagg aagtcgactg gctcgacctg ctgaccgccg aggacctcga
     4441 cgcgatgctg gcaatgccgg agatcggtca cgacggcggc gagggtgaca gcaccttcta
     4501 caccccgggc ggcctcaagc agctgccgaa cctgccggcg gtgatgtatt cgacgcgcac
     4561 ggtggccgcg ctggacgagc aggcgctgag cctggtcggc tacccggtgc cgctggagcg
     4621 cgaggcgggc agcccgctgc ggcgcttctt gctgctgccc tatccgggcg cctgcaacca
     4681 cctgccgccg ccgccaccca accagatcgt gctggtgagc tatcccgccg gcatcgccgt
     4741 cgacgacccc tacgtgccgc tgcgcgtcag cggcacgctg aaggtccagg cgcagggcga
     4801 cgcggcctac acgctggacg ccgacagcgt cggcgcggtc gaggagatcg agctgtagcc
     4861 gaccggcccg ccgcgccgcg ccctcctgtc actcgatgtg gctgccgctg gcgaccgtct
     4921 tgcgatagga cttggcaccc gccggcaccg ggctgccctt gccggtctcc agccagcgac
     4981 ggacgcgggt ggcgtcgccg atatggctgc gcttgccgct ggtatccagc agcaccatgg
     5041 ccaccgggcg attggcgacc cgggtgagca gcgccaggca gtggccggcc tcgttgatga
     5101 agccggtctt ggtgacctcg atgtcccagc cggggttgcg caccagcggg ttggtgttgt
     5161 tgaaacccag cacgtagttg ggcttgcgga agctggcggt cttgctcggc gtggtgctca
     5221 gctcgcggat catcgggtac tgccgcgcgg ccttgagcag gcgcaccagg tcgcgggcgg
     5281 tggacacgtt gaactccgac aggccggtcg gctcgacgaa acgggtgcgg ttcatgccca
     5341 gcgccttggc cttggcattc atcgccgcga cgaagcgcgc atggccaccc gggtagttgt
     5401 gcgccagggc ggaggcggcg cggttttccg acgacatcag ggccaggcgc agcatttcct
     5461 tgcggctgat ctggctgttg acccgcaccc gcgagtagac gccgcgcagc tccttggtgt
     5521 cgtggatggt caccgggatg acctcgtcca tcggcagctg ggcatcgagg gtcaccaccg
     5581 cggtcatcag cttgctgatc gaggcgatgg gccgcacgct gtccggcttg tcggcgaaca
     5641 gcacctggtc gctgtccagg tcgaccacca tggcactgct ggaagccagc tgcaggcggg
     5701 ccgggtcgtg cgccgcggcc gggccggcgg catgggcggt cggggtcaga gcaatccatg
     5761 cgcatgacaa cagtaaagtg aatagggagg agcgaatctg ggtgcgcatg gcgatggctt
     5821 cgcatggatg aaagaaaaac gtgtcgtatt gcgggtcaca gtatagtgaa ctggctcgaa
     5881 ttccgtggcc tctgccggcg catctttacc ctcgattgac tttcccgtgc ctgcgcctca
     5941 aagatgtgca aaaaaagcaa cgaggtgttc aatcatgagc tccagtggaa tcgccgaaca
     6001 cgccgtggaa gccctcagcg cgcgcgagcg cgagcgcttc atcgccgcca acccgcagtc
     6061 cgtggcgctg gccgagcgcg cccgccagtc actgttcggc ggcgtgccga tgcactggat
     6121 gagcgactgg tcgacgccgg caccgctgtt cgtcgagagc gcccagggcg cccgcttcct
     6181 cgacgtcgac ggtcaccaat acctcgactt ctgcctcggc gacaccggca gcatgttcgg
     6241 ccattcgccg gcgccgattg ccgccgccct gcgcgaacag gccggtcgcg gcctgaccac
     6301 catgctgccc ggcgaggacg cggtggcctg cggcgagctg ctggcggcgc gcttcggcct
     6361 gccgttctgg caggtggtga ccaccgccac cgacgccaac cgcttcgtcc tgcgctgggc
     6421 gcgggcgatc accgggcgca aggtgatcct ggtgttcgac ggctgctacc acggcacggt
     6481 cgacgagacc ctggtgcgcc tgcgcgacgg ccacaccgtg caccggccgg ggctgatcgg
     6541 ccaggcgcgc aacctgaccc agcacagccg cgtggtgccg ttcaacgacc tcgccgcgct
     6601 ggaagcggcg ctggcgcccg gcgacgtcgc cgccgtgctc tgcgagccgg cgctgaccaa
     6661 catcggcatg gtgctgccgc agcccggctt catggcccgg ctgcgcgagc tgacccggca
     6721 ggccggcagc ctgctgatcg tcgacgagac ccacacgctg tccaccggcc ccggcggctg
     6781 cacccgcgcc tggcagctcg agccggactt cctcaccctc ggcaaggcca tcgccggcgg
     6841 cgtgccctgc gcggtgtacg gctgcagcgc ggaaatggcc caggccatgc agctgacccg
     6901 cgcccactcg cgcgagatgg ccgagggcca cggccacagc ggcatgggca ccaccctgtc
     6961 ggccaacgcc ctggccatgc actgcctgcg cgccaacctc gagcaggtga tgactgcccc
     7021 ggcctacgag cacatgctgt ggctggcggc gcgcctggcc gagggcttcc gggcgctgat
     7081 cgcccgccac gcgctgccct ggtcggtgac cgagctgggc gcgcgctgcg agttccagtt
     7141 ccgcgccacg ccgccgcaga acggcgccga ggccgaggcg gccttccacg acagcctgca
     7201 gatggccctg cacctgtacc tgatcaaccg cggcatcctg atcacgccgt tccacaacat
     7261 gaccctgtgc tgcccggaaa ccagcgccgg cgacgtcgac cagctgctcg ccgccctcga
     7321 cgcggcgctg agcgagctgc tggccatgcc cggcgcgcgg caggcctgag ccatggcccg
     7381 ctatccctcc ggcat
//
LOCUS       DATCUN010000213         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_178997, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000213 DATCUN010000000
VERSION     DATCUN010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178997"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VIO83_08390"
     CDS             complement(<1..220)
                     /locus_tag="VIO83_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8331326.1"
                     /translation="MATRFQVDVVDYPTQREALHRVRQRVFVEEQQVPAALEIDALDP
                     LSAHVLARGSDGEPIGAGRLTPDGRIGRM"
     gene            complement(223..1641)
                     /locus_tag="VIO83_08395"
     CDS             complement(223..1641)
                     /locus_tag="VIO83_08395"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8331327.1"
                     /translation="MAARTTKTPVIEIQARPGLPLGMPAAVFLRDYWQKRPLLIRGAF
                     PDFETPVQPEDLAGLACEETALSRLITHDRATDGWSVRTGPFQEDEFPGMPDHDWTLL
                     VQDVDKWDADVRALVNHFDFLPRWRMDDVMISFAATGGSVGAHVDQYDVFLLQAYGHR
                     RWQIDASESTKGKRPPLDFRDDVELKLLRRFKPTHDWVLEPGDMLYLPPNVPHNGVAE
                     DPCLTFSFGMRAPASAELISDYLDTLIEGADESIRYQDPDLKLPEDPNEIDVLAMNRV
                     VEALNAIRMNDPDKLGDWFGRFITTYRAAGDVVAGADPLPREEIEAALATGLDLQRHP
                     WARMAWRRAKRGASLYCSGLEFALPVKDAQVLAATEQLGGPLYLKLSAKGRDAVQALV
                     AGGYYQLFDPNAIDEDFDDEEDEEDGHFAEDAVVDGTDSIEVMHDATVEADVHEVTIH
                     EDGIEVIVDFDDSDDQDDGKPV"
BASE COUNT          391 a          811 c         1018 g          468 t
ORIGIN      
        1 ccatgcggcc gatccggccg tccggggtga gccgtccggc accgatcggt tcgccgtcgc
       61 tgccacgggc caggacgtgg gcgctgagcg ggtccagcgc gtcgatttcc agcgcggccg
      121 gcacctgctg ttcctcgacg aacacgcgct ggcggacccg atgcagcgcc tcgcgctgcg
      181 tcgggtagtc cacgacgtcg acctggaagc gggtcgccat gctcagacgg gcttgccgtc
      241 gtcctggtcg tcgctgtcgt cgaaatcgac gatgacttcg atgccatctt cgtggatggt
      301 cacttcatgc acgtcggcct cgacggtggc gtcgtgcata acctcgatgc tgtcggtgcc
      361 gtcaaccacg gcgtcctcgg cgaagtggcc gtcctcttcg tcctcttcgt catcgaaatc
      421 ctcgtcgatg gcgttggggt cgaacagctg gtaatagccg ccggccacca gggcctgcac
      481 ggcgtcgcgg cccttggccg agagcttgag gtagagcggg ccgccgagct gttcggtggc
      541 cgccaggacc tgcgcatcct tgaccgggag ggcaaactcc aggccgctgc agtacaggct
      601 ggccccgcgc ttggcgcggc gccaggccat gcgtgcccac gggtggcgct gcaggtccag
      661 gcccgtggcc agcgcggcct cgatttcttc gcgcggcagc gggtcggcac cggccaccac
      721 gtcgccggcg gcgcggtagg tggtgatgaa gcggccgaac cagtcgccga gcttgtcggg
      781 gtcgttcatg cggatggcat tgagcgcctc gaccacgcgg ttcatggcca gcacgtcgat
      841 ttcgttgggg tcttcgggca gcttgagatc cggatcctgg tagcggatcg actcgtcagc
      901 accctcgatg agggtgtcca ggtagtcgct gatcagctcg gccgaggccg gggcgcgcat
      961 gccgaaggag aaggtcaggc aggggtcttc ggccacaccg ttgtgcggga cgttgggcgg
     1021 caggtagagc atgtcgcccg gttccagcac ccagtcgtgg gtgggcttga agcggcgcag
     1081 gagcttgagt tcgacgtcgt cgcggaagtc cagcggcggg cgcttgccct tggtggattc
     1141 actggcgtcg atctgccagc ggcggtggcc gtaagcctgc agcaggaaga cgtcgtactg
     1201 gtcgacgtgg gcgccgaccg agccgccggt ggcggcgaag ctgatcatca catcgtccat
     1261 gcgccagcgc ggcaggaagt cgaagtggtt gacgagggcg cggacgtcgg cgtcccactt
     1321 gtcgacgtcc tggacgagca gggtccagtc gtggtcgggc atgccgggga attcgtcttc
     1381 ctggaacggg ccggtacgca cgctccagcc gtcggtggcg cggtcatggg tgatgaggcg
     1441 cgacagggcg gtttcttcgc aggccaggcc ggcgaggtct tcgggctgga cgggggtttc
     1501 gaaatcgggg aaggcgccgc ggatgagcag cgggcgcttc tgccagtagt cgcgcaggaa
     1561 gacggcggcg ggcatgccga gcggcaggcc ggggcgggcc tggatttcga tgacgggggt
     1621 cttggtggtg cgggcagcca tgggggaatc cttggagcgt gtagacggaa agcgggggcg
     1681 cggcaggtgg ccggcgcgag cggggccatt gtccgctggt tggggccgat gtgcacctgc
     1741 ggtggggtgt cgcggggtgg tgggggtgga gtttggaagc gtaaggccag gtccaaagct
     1801 acgtccaaag cgacgtccag agcgacgtcc agagcgacgt ccagagcagc gccttgactg
     1861 actgcggcgg gaggtggccg ggcagccttg cccaggacac gccgtaaacc cgtccctggg
     1921 ggctcggtgt tgccatccat ggcaacacac ggtcctgggc aaggccgccc ggccacctct
     1981 ggacggtatc cggtgtgggc ggcaggtaag gcgggtcagt ggcctgcgtt gttacgtgac
     2041 gtgcgcggta agcgaggtac gacagaggtg atgtgttgct ttggtagggt cgggtcccaa
     2101 ccgattgccg agaaggggtc ctgatccggt aacgcattgc aatgagacgc ccgtagagcc
     2161 gggctctgcc cggctgctct atggcctacg acgtaaaacg cacgctggcg gtacagctgc
     2221 ccgtctttct ccggccaagg tcatgcgttc gtcgcgttga tcagcagtat cggcggtcga
     2281 tcgggatccg accctaccgg gttggtcggt ggcgggcctg tccgcggcct ttgtcatgcg
     2341 gggatatgcg cgggtcccgc accgggcgac gtcggctcgg gcgatgcccc gcagccggtt
     2401 tagtccgtgg cttcgcggcg acgcaaggcc ggccggcgca tggcgctggt agggtcgggt
     2461 cccaaccgac tgccgagaag ggatcccgat ccggtaacgc attgcaatga aacgccccgt
     2521 agcgccgggc tctgcccggc tgccccgcag cccacaacga agaagggacg caggcagtgc
     2581 cggcgtccct tctggggtga tgcgggtggc gcgatcctca gatcgccttg gccaggtccg
     2641 ccgccaggcc gatgtagccg cccggggtca ggtcgcgcag gttctgct
//
LOCUS       DATCUN010000214         4148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_83983, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000214 DATCUN010000000
VERSION     DATCUN010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83983"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VIO83_08400"
     CDS             complement(<1..387)
                     /locus_tag="VIO83_08400"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY8331328.1"
                     /translation="MKGKPRLVLPLLCCLVAGVLLAGCATMTSSTGRRQNVGAVSQQE
                     LDQLGAQAFAEAKQKQAISRDPGQNAYVRCVVNALVAQLPASTRGIAWETAVFEDSDA
                     NAFALPGGKVGVNTGMFPVARTQDQLA"
     gene            complement(439..1281)
                     /locus_tag="VIO83_08405"
     CDS             complement(439..1281)
                     /locus_tag="VIO83_08405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY8331329.1"
                     /translation="MEADPVQSGGGQGASSRPGSALPLRQALSRFALEVLIVAVLFGL
                     LSLSGALAWGLWRGVQLALQGMPTADPGAIMQAVGKPGPLATIAMSVLGVGGTALAAM
                     LWRAPPRVDERRRSRAVLRMPETWLLALFTAAVVICFSAVVSQTAAALRQSLDPTNMT
                     LVRGTLAQHPGLLLLFAVVAAPIYEEVLFRRVLFGRLWRDGWPLLGAVLSGVLFAFMH
                     EPPWMGDKSLAAQLPLWVVYTCMGIAFAWVYRRTGSLGASIIAHGLNNAFALGLMMAF
                     GVAR"
     gene            complement(1293..3767)
                     /gene="gyrB"
                     /locus_tag="VIO83_08410"
     CDS             complement(1293..3767)
                     /gene="gyrB"
                     /locus_tag="VIO83_08410"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEY8331330.1"
                     /translation="MTDETNGNGSGNGQAEYGAESIKVLKGLDAVRKRPGMYIGDTDD
                     GSGLHHMVYEVVDNAIDETLAGWASRVEVILNADGSCTVTDDGRGIPVDIHEGEGVSA
                     AEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSDWLKLVIYRGGLKHEMEFRRG
                     DAVSPLVITGKAPLRPNGEFLSGTEVTFLPSTDTFAFIDFDRKTLEHRLRELAFLNSG
                     VTIYLRDLRDAEPFEEVLHYDGGIAAFVQHLDKAKTALLKAPIMIRGVRENVEVDLSL
                     WWNDGYHENVLCFTNNIPQRDGGTHLSAFRSALTRIITGYAEASGAAKREKVSVSGED
                     AREGLTCVLSVKVPDPKFSSQTKDKLVSSEVRPAVEGLVSEGLGQWFEEHPTEAKLIV
                     QKIAEAAAAREAARKARELTRRKSALDITSLPGKLADCQERDPAKSEIFLVEGDSAGG
                     SAKQARNRENQAVLPLRGKILNVERARFDKMLSSDQIGTLITALGAGIGRDDFDIEKI
                     RYHKIVVMTDADVDGAHIRTLLLTFFYRQMPELIERGHVYIGLPPLYKLKQGKQELYL
                     KDDAALNVYLANSAVENAALIPAAGEPPISGEVLEKLLILFANARDTIARNTHRYNPA
                     LLEAMIDAAPTDVLPSLDPAALRKELDTLEGALNSPSIGSPRYTLELQTATDTRPAAL
                     LATRHHMGEELTQVLSLSAFETGELRPLREAAAQLHGLVRDGARIQRGARSQEITSFA
                     QAQAWLLEEAKKGRQIQRFKGLGEMNPEQLWETTVNPDTRRMLQVRIEDAVAADQIFS
                     TLMGDVVEPRREFIDANALKVSNLDI"
     gene            complement(3764..>4148)
                     /locus_tag="VIO83_08415"
     CDS             complement(3764..>4148)
                     /locus_tag="VIO83_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331331.1"
                     /translation="AARDRDAAAGRSLTGPHRGDFEAIHAEKDRPAAECSTGEQKALI
                     LNLVLAQAARLSGAESAPVPILLLDEVAAHLDRRRRAALFDEILALELQAFLTGTDEH
                     LFEDLKGRALGVRVDASGLSILEDE"
BASE COUNT          646 a         1358 c         1442 g          702 t
ORIGIN      
        1 ggccagctga tcctgggtcc gggccaccgg aaacatgccg gtgttcactc cgaccttgcc
       61 gccgggcagg gcgaaggcgt tggcgtcgct gtcctcgaac accgccgttt cccaggcgat
      121 gccgcgtgtg gatgccggca gctgggccac cagcgcgttg accacgcagc gcacgtaggc
      181 gttctgtccg ggatccctgc tgatggcctg tttctgcttg gcctcggcga aggcctgggc
      241 gccgagctga tccagttcct gctgcgagac cgcgcccacg ttctggcgac ggccggtcga
      301 ggaggtcatg gtggcgcaac cggccagcaa cacgccggcg accaggcagc acaacagcgg
      361 gaggacgagt cggggtttgc ctttcatgcg gatcagtgtt ggcatacaaa ccatgaactt
      421 tccgtcaagc ttccgcgctc agcgggcgac gccgaaggcc atcatcaggc cgagcgcaaa
      481 agcgttgttg agcccgtgcg cgatgatcga tgcccccagg gagccagtgc gccggtagac
      541 ccacgcaaag gcgatgccca tgcaggtgta gaccacccac agcggcagtt gcgcggccag
      601 cgacttgtcg cccatccatg gaggttcgtg catgaaggcg aacagcaccc cactcaacac
      661 cgcgcccagc aatggccagc cgtcacgcca gagcctgcca aacagcacgc gccggaacag
      721 gacttcttcg tagatcggcg cggcgaccac ggcgaacagc agcagcagcc cgggatgctg
      781 ggccagcgtg ccgcgcacca gggtcatgtt ggtgggatcc agcgattggc gcagggccgc
      841 ggcggtctgc gagacgaccg cgctgaagca gatcaccacc gcggcggtga acaacgccag
      901 cagccaggtc tccggcatgc gcaggacggc acgcgagcgg cgacgttcat cgacgcgggg
      961 cggggcgcgc cagagcatgg ccgccagcgc cgtgccgccc accccgagca cgctcatggc
     1021 gatggtcgcc agcgggccgg gcttgccgac tgcctgcatg atcgcgccgg gatcggcggt
     1081 gggcatgccc tgcagcgcca gctgcacgcc gcgccagagg ccccaggcca gggcgcccga
     1141 cagcgaaagc aggccaaaca gcaccgccac gatcagcacc tccagcgcga agcgcgacag
     1201 cgcctggcgc agcggcagtg cgcttcccgg ccgggaagag gctccctgcc cgccgccgga
     1261 ctggaccgga tcggcttcca tgcgcgccag gctcagatgt ccaggttgga caccttcagc
     1321 gcattggcgt cgatgaactc gcggcgcggc tccaccacat cgcccatcag cgtgctgaag
     1381 atctggtcgg cggccaccgc gtcctcgatc cggacctgga gcatgcgccg ggtatcggga
     1441 ttgaccgtgg tttcccacag ctgctcgggg ttcatttcgc ccaggccctt gaagcgctgg
     1501 atctggcggc ccttcttggc ttcctccagc agccaggcct gggcctgggc gaagctggtg
     1561 atctcctggc tgcgggcgcc acgctggatc cgggcgccgt cgcggaccag gccgtgcagc
     1621 tgcgcggccg cttcacgcag cgggcgcagt tcgccggttt cgaaggccga cagtgatagc
     1681 acctgggtca gttcctcgcc catgtggtgg cgggtggcca gcagcgcggc cgggcgtgtg
     1741 tcggtggcag tctgcagttc cagggtatag cgcgggctgc cgatgctggg gctgttgagc
     1801 gcgccttcca gggtgtccag ctccttgcgc agggccgcag gatccaggct gggcaggaca
     1861 tcggtcggcg cggcatcgat catcgcctcg agcagggccg gattgtagcg atgtgtattg
     1921 cgcgcgatcg tatcgcgtgc attggcgaac aggatcagca gtttttccag gacctcgccg
     1981 gaaatgggcg gctcgccggc ggccgggatc agcgcggcat tctccaccgc gctgttggct
     2041 aggtagacgt tgagcgccgc gtcgtccttc aggtacagct cctgcttgcc ctgcttgagc
     2101 ttgtacagcg gcggcaggcc gatgtagacg tggccgcgct cgatcagctc gggcatctgc
     2161 cggtagaaga aggtcagcag cagggtgcgg atatgggcgc cgtcgacgtc ggcgtcggtc
     2221 atgaccacaa tcttgtggta gcggatcttc tcgatgtcga agtcgtcgcg gccgatcccc
     2281 gcgcccagcg cggtgatcag cgtgccgatc tggtcggacg aaagcatctt gtcgaagcgg
     2341 gcgcgctcga cgttgaggat cttgccccgc agcggcagca ccgcctggtt ctcgcggttc
     2401 ctggcctgtt tggccgagcc gccggcggaa tcgccctcga ccaggaagat ctctgacttg
     2461 gccggatcgc gttcctgaca gtcggcgagc ttgccgggca gcgaggtgat gtcgagcgcc
     2521 gacttgcggc gggtgagctc gcgggccttg cgggcggctt cacgggcggc ggcggcctcg
     2581 gcgatcttct ggacgatcag cttggcctcg gtcgggtgct cctcgaacca ttggccgagt
     2641 ccttcggaca ccaggccctc gaccgccggg cgaacctcgg aggagaccag cttgtccttg
     2701 gtctgcgagg agaacttcgg gtcgggcacc ttgaccgaca gcacgcaggt cagcccctcg
     2761 cgcgcgtcct cgccggagac cgacaccttc tcgcgcttgg ccgccccgct cgcttcggcg
     2821 tagccggtga tgatccgggt cagcgccgag cggaaggccg acaggtgggt gccgccgtcg
     2881 cgctggggga tgttgttggt gaagcacagg acgttctcgt ggtagccgtc gttccaccac
     2941 agcgacaggt cgacctcgac gttctcgcgc acgccgcgga tcatgatcgg cgccttgagc
     3001 agcgcggtct tggccttgtc caggtgctgg acgaaggcgg cgatgccccc gtcgtagtgc
     3061 agcacttcct cgaacggctc ggcgtcgcgc aggtcgcgca ggtagatggt cacgcccgag
     3121 ttcaggaagg cgagctcgcg cagccggtgc tccagcgtct tccggtcgaa gtcgatgaag
     3181 gcgaaggtgt cggtcgaggg caggaaggtg acctcggtgc ccgacaggaa ctcgccgttc
     3241 ggccgcaagg gcgctttgcc ggtgatcacc agcggcgaga cagcatcgcc ccggcggaac
     3301 tccatctcgt gcttgaggcc gccgcggtag atgaccagct tgagccagtc cgacagggcg
     3361 ttgaccaccg agacgccgac gccgtgcagg ccgccggaga ccttatagct gttctggtcg
     3421 aatttcccgc cggcgtgcag ctgggtcatg atgacctcgg ccgccgagac gccctcgccc
     3481 tcgtggatgt ccaccgggat gccgcggccg tcgtcggtga cggtgcacga gccgtcggcg
     3541 ttgaggatca cctcgacgcg gctggcccag ccggccagcg tctcgtcgat ggcgttgtcg
     3601 accacctcgt agaccatgtg gtgcaggccc gagccgtcgt cggtgtcgcc gatgtacatg
     3661 ccggggcgtt tgcgcacggc atcgaggccc ttgaggacct tgatcgattc cgcgccatat
     3721 tcggcctggc cgttgccact gccgttgccg ttcgtctcgt cggtcattcg tcttccaaga
     3781 tggaaaggcc ggaggcgtcc acgcgaacgc ccagggctcg tcctttcaag tcctcgaaca
     3841 ggtgctcgtc cgtgccggtg agaaaggcct ggagctcgag cgccagtatt tcgtcgaaca
     3901 atgcggctcg ccggcggcgg tccagatgcg ctgcgacttc gtccagcaac aatataggaa
     3961 ctggcgctga ttctgcacct gaaagacggg ctgcctgggc caaaacaagg ttcaaaatca
     4021 gggctttttg ctctccggtg gagcattcgg cggccgggcg gtccttctcg gcgtggatgg
     4081 cttcgaaatc gccccggtgc gggccggtca gcgatcggcc ggcggcggcg tcgcggtcgc
     4141 gcgcagcc
//
LOCUS       DATCUN010000215         6520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_254223, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000215 DATCUN010000000
VERSION     DATCUN010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254223"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /gene="aceE"
                     /locus_tag="VIO83_08420"
     CDS             complement(<1..365)
                     /gene="aceE"
                     /locus_tag="VIO83_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253702.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY8331332.1"
                     /translation="MQDLDPVETQEWLDALESVLDREGETRAHYLLTRLGEQASRTGT
                     QLPYAITTPYRNTIPVTHEARMPGDLFMERRIRSLIRWNALATVMRANKKDPDLGGHI
                     STFASSATLYDIGFNYFFKA"
     gene            633..3569
                     /gene="glnE"
                     /locus_tag="VIO83_08425"
     CDS             633..3569
                     /gene="glnE"
                     /locus_tag="VIO83_08425"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016972664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEY8331333.1"
                     /translation="MSLPTLTALPAELHALVEHARQTFVSRLDEGGQTALAAWPPERR
                     EALERVLGASDFVAEQAARDPQMLLELAASGELERALAAGELREQLGQALAGCSSEDE
                     LARCLRRYRNRQQSRIIWRDVNRLADLAETCRDLSELADACIDLAYHWLYARLCEQSG
                     TPVGARSGEAQHLVVLGMGKLGAFELNLSSDIDLIFAYPEGGETVGARRALDNQEFFT
                     RLGQKLIKALDAVTVDGFVFRVDMRLRPYGSSGPLVYSFNAMEQYYQDQGRDWERYAM
                     IKARVVAGDQAAGAQLQQMLRPFVYRRYLDFSAIEALRTMKQLIQQEVRRKGMTENIK
                     LGAGGIREVEFIAQAFQLIHGGRDLSLQQRPLLKVLATLAGQGYLPAPVVAELTEGYC
                     FLRYLEHALQAIADRQTQMLPDGEQERARVAFIMGFDGWNALLDRLAYWRGRIDFHFR
                     QVIADPDDEAGANLAGIGDEWLPLWQNALEAEAAQHQLREAGFADAAVAWQRIEGLQH
                     ASPVRAMQRLGRERLDAFMPRLLAAMVAHGTPDLLLERLLPLVEAVARRSAYLVLLSE
                     NPGALERLLTLCAASPWVAEQIARFPLLLDELLNEGRLYSPPGKAELTAELRERLTRI
                     PEDDLEQQMEALRHFKLAHGLRVAASEIAGSLPLMKVSDYLTWLAEAILEQVLQLGWR
                     QVGQRHGYPRRADGSVASEPDFIIVGYGKVGGIELGHSSDLDLVFVHSGDQQTETDGA
                     KPIDSAQFYTRLGQRIIHLLTAQTASGALYEVDMRLRPSGVSGLLASSLAAFERYQES
                     EAWTWEHQALVRARKLTGSEGVAEQFEQIRARVLGRERDGAALRAEVSEMRAKMRDNL
                     GTKSTAGGTAAEAFDAAATFNLKHDAGGIVDIEFMVQYAVLAWSRQYPELLRFTDNIR
                     ILEGLERAGLIAGADVHLLQEAYKAYRAAAHRLALQKQAGVVSGDHFHEERRGVMRIW
                     RELGLA"
     gene            3689..4612
                     /locus_tag="VIO83_08430"
     CDS             3689..4612
                     /locus_tag="VIO83_08430"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEY8331334.1"
                     /translation="MSMADRDGVIWYDGEMVQWRNATTHVLTHSLHYGMGVFEGVRAY
                     NTPQGTAIFRLEAHTDRLFDSAHIMGMKIPFTKDEINEATKAAVRDNGLESAYIRPLA
                     FYGSEGMGIRADNLKVHVIVAAWHWGAYMGDEALENGIKIRTSSFTRHHVNITMTRAK
                     SSGAYVNSMLALQEAVSAGADEALLLDPEGYVAEGSGENVFIVKNGVIYTPEVTACLN
                     GITRNTVLTLAREQGIEIVEKRITRDEVYIADEAFFTGTAAEVTPIREVDGRQIGIGR
                     RGPVTEKLQKAYFDLVTGKTDAHAEWRTLVK"
     gene            4700..5749
                     /gene="waaF"
                     /locus_tag="VIO83_08435"
     CDS             4700..5749
                     /gene="waaF"
                     /locus_tag="VIO83_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEY8331335.1"
                     /translation="MRILIVGPSWVGDMVMAQTLFQCLKQRHADCQIDVLAPDWSRPI
                     LERMPEVRRALSFPFGHGVFDLAGRRRIGKSLAGQYDQAILLPNSLKSALVPFFAGIP
                     LRTGWRGEMRYGLLNDIRTLDEQRYPLMIERFMALAYAPGAALAQPYPRPRLEIDPVS
                     REAALHKFGLSLDRPVLVLCPGAEFGEAKRWPAEHYAAVADAKIRAGWQVWLFGSKSD
                     HPVGETIREELIPGLQEEAVNLAGETSLAEAIDLMSCADAVVSNDSGLMHVAAALNRP
                     LVAVYGSTSPQFTPPLAEQVEIVRLGLDCSPCFERTCRFGHYNCLRDLQPQPVLDALQ
                     RLAGEPLADPLPQEH"
     gene            5750..>6520
                     /gene="waaC"
                     /locus_tag="VIO83_08440"
     CDS             5750..>6520
                     /gene="waaC"
                     /locus_tag="VIO83_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEY8331336.1"
                     /translation="MRVLLIKTSSLGDVIHSLPALTDAMRAIPGIRFDWVVEEGFAEI
                     PTWHPAVDRVIPVALRRWRKNLWSTLRGGEWRQFKARLNEQRYDLVIDAQGLLKSALL
                     TRYVQAPVAGLDRASAREPLASRFYDRGCTVPWGVHAVERVRQLFAQALGYPLPAETG
                     DYALDRQRLLAGRNDEPYLLFLHGTTWVTKHWPERYWRELAERLSAAGWAIRLPWGNA
                     EEQARAERIAAGLDRVSVLPKLNLAGVAAELATATQDVP"
BASE COUNT         1055 a         2248 c         2223 g          994 t
ORIGIN      
        1 gccttgaaga aatagttgaa gccgatgtcg tacagggtcg cactggaggc gaaggtggag
       61 atgtggccac ccaggtccgg gtccttcttg ttcgcgcgca tcaccgtggc cagggcgttc
      121 cagcggatca gcgagcggat gcgccgttcc atgaacaggt cgcccggcat gcgtgcctcg
      181 tgggtcaccg ggatggtgtt gcgatacggc gtggtgatgg cgtacggcag ctgggtgccg
      241 gtgcgcgaag cctgctcgcc cagacgggtc agcaggtagt gagcgcgggt ttcaccttca
      301 cgatcgagga cggactccag ggcgtccagc cattcctggg tttcgacggg atcgagatct
      361 tgcatggctt gctccagggc ggaaaggctt ccagaatcgg aggccgtata taaagcgcgg
      421 tcgggcttgt gggcgactgc ggcgaatttt gtggttgtcg gggcgcgacg ctgttgcagc
      481 gtgctccacc tgccaggggt cacccggcga cccgtgtagt tttactacat gtttgcggca
      541 aaagcagctc cctgcgacaa aagtttagta gcaaaactac agctaacgac gaccggccag
      601 atggccgtcg cggcgcgata aaaggataga ccatgagcct ccccaccctg actgccctgc
      661 ccgcggaact ccatgccctg gtcgagcacg cccggcagac cttcgtctcg cgcctcgacg
      721 agggcgggca aaccgcgctg gcggcctggc cgccggagcg ccgcgaggcg ctggagcggg
      781 tgctgggggc cagcgatttc gtcgccgaac aggcggcgcg cgacccgcag atgctcctcg
      841 agctggccgc cagcggcgag ctggagcgcg ccctggcagc aggcgaactg cgcgagcaac
      901 tgggccaggc gctggccggc tgcagcagcg aggacgaact ggcgcgctgc ctgcgccgct
      961 accgcaaccg ccagcagagc cggatcatct ggcgcgacgt caaccgcctg gccgacctcg
     1021 ccgagacctg ccgcgatctc tccgagctgg ctgatgcctg catcgacctg gcctaccact
     1081 ggttgtacgc gcggctgtgc gagcagtccg gcacgccggt cggcgcgcgc agtggcgagg
     1141 cccagcacct ggtggtgctc ggcatgggca agctcggcgc cttcgagctc aacctgtcct
     1201 cggacatcga cctgatcttc gcctaccccg agggcggcga gaccgtcggc gcgcgccgcg
     1261 cgctggacaa ccaggagttc ttcacccgcc tgggacagaa gctgatcaag gcgctggatg
     1321 cggtgaccgt cgacggcttc gtgttccgcg tcgacatgcg cctgcgcccc tacggctcca
     1381 gcggcccgct ggtgtacagc ttcaacgcca tggagcagta ctaccaggac cagggccgcg
     1441 actgggagcg ctacgcgatg atcaaggcgc gcgtggtggc cggcgaccag gccgccggcg
     1501 cgcagctgca gcagatgctg cggccgttcg tctatcgccg ctacctggac ttctcggcca
     1561 tcgaggcgct gcgcaccatg aagcagctga tccagcagga ggtgcgccgc aagggcatga
     1621 ccgagaacat caagctcggt gccggcggca tccgcgaggt ggagttcatc gcccaggcct
     1681 tccagctgat ccacggcggc cgcgacctca gcctgcagca gcgcccgctg ctcaaggtgc
     1741 tcgccaccct ggccggccag ggctacctgc cggcgccggt ggtggccgag ctgaccgaag
     1801 gctactgctt cctgcgctac ctggagcacg ccctgcaggc catcgccgac cgccagacgc
     1861 agatgctccc cgacggcgag caggagcgcg cgcgggtcgc cttcatcatg ggcttcgacg
     1921 gctggaatgc gctgctcgac cgcctggcct actggcgcgg gcgcatcgac ttccatttcc
     1981 gccaggtgat tgccgatccc gacgacgagg ccggggccaa tctggccggc atcggcgacg
     2041 agtggctgcc gctgtggcag aacgccctgg aggcggaagc cgcccagcac cagctgcgcg
     2101 aagccggctt cgccgatgcg gcggtcgcct ggcagcgcat cgagggcctg cagcatgcct
     2161 cgccggtgcg cgccatgcag cgcctcggcc gcgagcggct ggacgccttc atgccgcgcc
     2221 tgctggcggc gatggtcgcc cacggcactc cggacctgct gctcgaacgt ctgctgccgc
     2281 tggtcgaggc ggtggcgcgg cgctcggcgt acctggtgct gctcagcgag aaccctgggg
     2341 cgctggagcg cctgctgacc ctgtgcgcgg ccagcccgtg ggtggccgag cagatcgccc
     2401 gtttcccgct gctgctcgac gagctgctca acgaggggcg gctgtacagt ccgcccggca
     2461 aggccgagct gaccgccgag ctgcgcgagc ggctgacgcg cattcccgag gacgacctcg
     2521 agcagcagat ggaggcgctg cgccacttca agctggccca cggcctgcgc gtggccgcct
     2581 cggagatcgc cggcagcctg ccgctgatga aggtcagcga ctacctgacc tggctggccg
     2641 aggcgatcct cgagcaggtc ctgcagctgg gctggcgcca ggtcggccag cgccacggct
     2701 acccgcgccg cgccgacggc agcgtggcca gcgagccgga cttcatcatc gtcggctacg
     2761 gcaaggtcgg cggcatcgag ctgggccaca gctccgacct cgacctggtg ttcgtccaca
     2821 gcggcgacca gcagaccgag accgacggcg ccaagccgat cgacagcgcg cagttctaca
     2881 cccgcctggg ccagcgcatc atccacctgc tcaccgcgca gaccgcctcc ggcgcgctgt
     2941 acgaagtgga catgcgcctg cgcccctccg gcgtgtccgg cctgctggcc agctcgctgg
     3001 cggccttcga gcgctaccag gaaagcgagg cctggacctg ggagcaccag gcgctggtgc
     3061 gcgcgcgcaa gctgaccggc tccgaggggg tcgccgagca gttcgagcag atccgcgccc
     3121 gggtgctcgg ccgcgagcgc gatggcgccg cgttgcgtgc cgaagtcagc gagatgcgcg
     3181 ccaagatgcg cgacaacctg ggcaccaaga gtaccgccgg cggcaccgcg gcggaggcgt
     3241 tcgatgcggc ggcgaccttc aatctcaagc acgatgccgg tggtatcgtc gatatcgaat
     3301 ttatggtgca atatgccgtt ctcgcctggt cgcggcagta ccctgaactg ctgcgtttca
     3361 ccgacaacat ccgcattctg gaagggctgg aacgggctgg cttgatcgcc ggcgccgacg
     3421 tccacctcct gcaggaggcc tacaaggcct atcgcgcagc tgcccaccgc ctggccttgc
     3481 agaaacaggc cggcgtcgtg agtggcgacc acttccacga ggagcgccgc ggcgtgatgc
     3541 ggatctggcg cgagctgggg ctcgcgtagg cgacaggtcg cgggcgatct gtctgccgca
     3601 ggcagaagtg cggcgcatga cgccaggcgt catgcggtcc gcgggtggac tgaaggcaaa
     3661 cacgaattct tgaaggagct ggcaaaaaat gtcgatggcc gatcgtgatg gcgtgatctg
     3721 gtatgacggt gaaatggtgc agtggcgcaa cgcgaccacc cacgtgctga cccacagcct
     3781 gcactacggc atgggcgtgt tcgaaggcgt gcgcgcctac aacacgccgc agggcaccgc
     3841 gatcttccgc ctggaagcgc acaccgaccg tctgttcgat tccgcccaca tcatgggcat
     3901 gaagattccg ttcaccaagg acgaaatcaa cgaggccacc aaggccgccg tgcgcgacaa
     3961 cggtctggaa agcgcctaca tccgtccgct ggccttctac ggttcggaag gcatgggcat
     4021 ccgcgccgac aacctgaaag tgcacgtgat cgtcgcagcc tggcactggg gcgcctacat
     4081 gggcgacgag gcgctggaaa acggcatcaa gatccgcacc agctcgttca cccgccacca
     4141 cgtcaacatc accatgaccc gtgccaagtc cagcggcgcc tacgtcaact cgatgctggc
     4201 cctgcaggaa gcggtgtccg ccggtgccga cgaggccctg ctgctggatc cggaaggcta
     4261 cgtggccgaa ggctcgggcg agaacgtgtt catcgtcaag aacggcgtga tctacacccc
     4321 cgaggtgacc gcctgcctga acggcatcac ccgcaacacc gtgctgaccc tggcccgcga
     4381 gcagggcatc gagatcgtcg agaagcgcat cacccgcgac gaggtgtaca tcgccgacga
     4441 ggccttcttc accggcaccg ccgccgaagt caccccgatc cgcgaagtgg acggccgcca
     4501 gatcggcatc ggccgccgcg gcccggtcac cgagaagctg cagaaggcct acttcgacct
     4561 ggtcaccggc aagaccgatg cccacgccga gtggcgcacc ctggtcaagt gaagcgagcg
     4621 tacgcttcac ggctggatgc gtgagtcaga aagccagtga cggggaggcg caagcctccc
     4681 tgttcatttc ggaaatcgca tgagaatact gatcgtaggt ccctcctggg tgggcgacat
     4741 ggtgatggcg cagacgctgt tccagtgcct gaagcagcgc catgccgact gccagatcga
     4801 cgtgctggct cccgactgga gccggccgat cctcgagcgc atgcccgagg tgcgccgggc
     4861 gctgagcttc ccgttcggcc acggcgtgtt cgaccttgcc ggccgccggc gcatcggcaa
     4921 gtcgctggcc ggccagtacg accaggcgat cctgctgccc aactcgctca aatccgcgct
     4981 ggtgccgttc ttcgccggca ttcccctgcg taccggctgg cgcggcgaga tgcgctacgg
     5041 tctgctcaac gacatccgca cgctcgacga gcagcgctat ccgctgatga tcgagcgctt
     5101 catggccctg gcctacgcgc ccggcgccgc gctggcgcag ccctatccgc gcccgcgcct
     5161 cgagatcgac ccagtcagcc gcgaggcagc gctgcacaag ttcggcctga gcctggaccg
     5221 cccggtgctg gtgctgtgcc ccggtgccga gttcggcgag gccaagcgct ggccggccga
     5281 gcactacgcc gcagtggccg acgccaagat ccgcgccggc tggcaggtct ggctgttcgg
     5341 ctcgaagagc gaccacccgg tgggcgagac gatccgcgag gaactgatcc ccggcctgca
     5401 ggaagaggcg gtcaacctgg ccggcgaaac cagcctggcc gaggccatcg acctgatgtc
     5461 ctgcgccgat gccgtggtgt ccaacgactc cgggctgatg cacgtggccg ctgccctgaa
     5521 ccgcccgctg gtggcggtgt acggctccac ctcgccgcag ttcaccccgc cgctggccga
     5581 gcaggtggaa atcgttcgtc tcggcctcga ctgcagcccg tgcttcgagc gcacctgccg
     5641 tttcggccac tacaactgcc tgcgcgacct gcagccgcag ccggtgctcg acgccctgca
     5701 gcgcctggcc ggcgagccgt tggccgatcc gttgccgcag gagcactgag tgcgcgttct
     5761 gctgatcaag acgtcgtcgc tgggcgacgt catccatagc ctgccggcgc tcaccgacgc
     5821 catgcgtgcg attcccggca tccgtttcga ctgggtggtg gaggagggtt tcgccgagat
     5881 ccccacctgg catccggcgg tcgaccgggt gatcccggtg gccctgcgcc gctggcgcaa
     5941 gaacctgtgg agcaccctgc gcggcggcga atggcggcag ttcaaggcgc gcctgaacga
     6001 gcagcgctac gacctggtga tcgacgccca ggggttgctg aagagcgcct tgctgacccg
     6061 ctacgtccag gccccggtcg ccgggctgga ccgcgccagc gcccgcgagc cgctggccag
     6121 ccgtttctac gatcgcggct gcaccgtgcc ctggggcgtg cacgcggtgg agcgggtgcg
     6181 ccagctgttc gcccaggccc tcggctatcc gctgccggcg gaaaccggcg actacgcgct
     6241 ggatcgccag cgcctgctgg ccgggcgcaa cgacgagccc tacctgctgt tcctgcacgg
     6301 caccacctgg gtcaccaagc actggccgga gcgctactgg cgcgagctgg ccgagcgcct
     6361 cagcgccgcc ggctgggcga tccgcctgcc gtggggcaat gccgaggagc aggcgcgcgc
     6421 cgagcgtatt gccgccggac tcgaccgggt cagcgtgctg ccgaaactga acctggccgg
     6481 cgttgccgcc gagctggcca ccgccacgca ggatgtgccg
//
LOCUS       DATCUN010000216         2329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_103649, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000216 DATCUN010000000
VERSION     DATCUN010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103649"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VIO83_08445"
     CDS             <1..1138
                     /locus_tag="VIO83_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8331337.1"
                     /translation="YEFELRQVSGGETIELGQATLQVMHTPGHTPEHISLQLFDAQQG
                     DQPIALFTGDTLFNLDVGRPDLLGEGSERKLAGALYHTLFDHYLPLGDRIEIYPCHGA
                     GSACGKSIGDRRHSTLGSERLFNPALREQRSEDAFIDWLLADMPEPPRHYARLKKLNL
                     RPAVQIEGPALPPPLAPSDFEALAAHDMQVVDVRSILAFGGGHVPGAVNIALRNEFVN
                     WAGRMLDDARPILLVGESADDVHQAVTQLYRIGLDDIRGYLRNGMTDWQNAALPLNRL
                     QEWTVHELNARREAPDVQVLDVRSPQEVAAGRVPGARHIFVAHLADRLNELDPDKTTV
                     TYCGSGYRASIAASILKKAGFRDVANVPGSWMAWKAANLPVQEG"
     gene            complement(1194..1976)
                     /locus_tag="VIO83_08450"
     CDS             complement(1194..1976)
                     /locus_tag="VIO83_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8331338.1"
                     /translation="MVEIGLGLLVGLVLALTGAGGGILTVPLLIFVVGLELHQAAPIG
                     LMAVGFAAWVGAVIGLRDGIVRYKAAALMACCGVLLAPVGLWVAARVAHDVLAVVFAL
                     VMFWVAFRAFRRPPSDDTTPRAVPCRLNPQTGRFHWTSRCAATVAASGTLAGFLSGLL
                     GVGGGFVLVPALSRFSDLPMRSIAATSLAVIGLVSVSGVLSAALTGGIQWAVALPFSA
                     GAVLGMFAGRTVSSRLDGRLLQRGFGLVAAVAASALLLQVAF"
     gene            complement(2031..>2329)
                     /locus_tag="VIO83_08455"
     CDS             complement(2031..>2329)
                     /locus_tag="VIO83_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEY8331339.1"
                     /translation="FVMGGAIAVASVGFYFASKRSRAVLGDIMRMPTATRIDRPLVLG
                     SLAFGAGWGLAGYCPGPAVATLLSGRSEPLIFVLAMLAGMALYELQTRFSGKPQ"
BASE COUNT          471 a          818 c          715 g          325 t
ORIGIN      
        1 ctacgagttc gagcttcgcc aggtcagcgg tggcgagacg atagagctcg ggcaggccac
       61 gctgcaggtc atgcacacgc ccgggcatac gcctgaacat atctccctgc agctgttcga
      121 cgcccagcag ggcgaccagc cgatcgcgct gtttaccggc gataccctgt tcaacctgga
      181 cgtcgggcgc cccgacctgc tgggcgaagg cagcgaacga aagctggccg gcgcgctgta
      241 ccacacactt ttcgatcact acctgcccct tggcgacagg attgaaatct acccctgcca
      301 cggcgccggc tcggcctgcg gcaagtctat cggcgaccgt cgccactcca cgctcggcag
      361 cgagcgtctc ttcaaccccg cgttgcgcga gcagcggagc gaggacgcgt tcatcgattg
      421 gctactggcg gacatgccgg aaccaccccg tcactacgcg cgcctgaaaa aactgaactt
      481 gcgcccagcg gtgcagatag aaggccctgc cctgccgccg cccctcgccc cgtccgactt
      541 cgaggcactg gccgcgcacg acatgcaggt tgtggacgtc cgctcgatcc tcgcgtttgg
      601 cggcgggcac gtgcccgggg cggtcaacat cgccctgcgc aacgagttcg tgaactgggc
      661 gggccggatg ctcgacgacg ctcgcccgat cctgctggtg ggcgaatcgg ccgacgatgt
      721 ccaccaggct gtcacccagc tctaccggat aggcctggac gatattcgcg gctatctgcg
      781 caacggcatg acggactggc agaacgcggc gctaccgctg aacaggcttc aggaatggac
      841 ggttcatgaa ctgaatgcac gtcgtgaagc gcccgatgta caggtgctgg atgtacgcag
      901 cccccaagag gttgcggcgg gtcgggtgcc tggcgccagg cacatcttcg tcgcgcacct
      961 cgccgaccgg ctcaacgagc tggatccgga caagaccacc gtcacctact gcggatccgg
     1021 ctatcgcgcc tccatcgctg ccagcatcct caagaaagcc ggctttcgtg atgtggccaa
     1081 tgtccccggt tcctggatgg cctggaaggc ggcaaacctg ccggtccagg aaggctaagg
     1141 tcttatccgg tcgcgcggcg caggtgtctg gcttgccacc tgcgggtgcg cgatcagaag
     1201 gcgacttgaa gcagcaaggc cgacgcggca acagccgcga ccaatccgaa gccccgctgc
     1261 agcaggcgcc cgtccaggcg agaggaaacc gtgcggccgg cgaacatccc gagcacggca
     1321 ccggccgaga atggcagcgc cactgcccac tggatgccac ccgtcagcgc ggcggacaac
     1381 acgccgctga ccgacaccag gccgatgacc gccaacgacg tggcggcaat cgaacgcatc
     1441 ggcagatcac tgaagcgact gagcgccggc accagcacga acccgccgcc gacccccagc
     1501 aagccggaga ggaaaccggc caaggtgccg gaggcggcga cggtcgctgc acagcgtgag
     1561 gtccagtgaa agcggcccgt ctgcgggttc agccgacagg gcacggctct cggggtcgtg
     1621 tcatcgctcg gcgggcgcct gaatgccctg aaggcgaccc agaacatcac cagcgcaaac
     1681 accacggcaa gcacatcgtg cgctacccgc gcggccaccc agaggccgac gggcgcgagc
     1741 aacacgccac agcaggccat cagggccgcc gccttgtacc ggacaatgcc gtccctcaga
     1801 ccaatcactg ccccgaccca ggccgcgaat ccgaccgcca tcaacccgat tggcgcggcc
     1861 tgatgcagct cgagcccgac cacgaagatc agcaacggca cggtgaggat gccgccgccg
     1921 gcaccggtca gcgccaggac gaggccgacc aatagcccaa gcccgatctc aaccatcgtt
     1981 gcgctccgga agccttggat cacctttcga tttagagcga ctgcaaccta tcactgcggc
     2041 ttccctgaga agcgtgtctg caactcgtag agcgccatac ccgccaacat ggcgaggaca
     2101 aagatcaagg gttcgctccg accactcaaa agcgtcgcga ccgccggacc cggacaatag
     2161 ccagccagcc cccatcccgc tccgaatgcc aggctgccca gtacaagagg gcgatcgatc
     2221 cgggtggcgg tgggcattcg catgatatct cccagcaccg cccgggaccg tttggaggcg
     2281 aaatagaacc cgacactggc gaccgcaatc gcaccgccca taacgaacg
//
LOCUS       DATCUN010000217         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_28447, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000217 DATCUN010000000
VERSION     DATCUN010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28447"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VIO83_08460"
     CDS             <1..406
                     /locus_tag="VIO83_08460"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY8331340.1"
                     /translation="AGLEFIRIAGPFTVLAEHLEQTVAREGFADFNAQGNTVYGSWVV
                     TGESRPYTGTLGNVTPKGPAGALELLLRYSDLDLNDAGLEGGYQHDWTVGANYYFTSH
                     FKVQVNYIKANSRRAGVEVDPNAFGIRAQVMF"
     gene            complement(435..1424)
                     /locus_tag="VIO83_08465"
     CDS             complement(435..1424)
                     /locus_tag="VIO83_08465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8331341.1"
                     /translation="MDPIASIVVPAYNAAGFIGRSIASIRQQTLKDLEILVVDDASTD
                     DTVATVKALQVHDQDIRLFEQDCNGGAAVARNTGIAQARGRYIAFLDADDIMLPQRLQ
                     HLIRHAEEQQLDIVADNQVLHDSYLDRQVGLLAFNESQHPQPLTALSFLKGSSNVPSL
                     REILSGSRAAYFPLIKPIIRRAFLTEHQLRYDPACRFGEDFDIIIRCLLEGARASIVP
                     QAYYVYTLSHSDVSNTRSPHSRTRFNMQPVIQNVDHLLRTYAHALTPQMRQALIRCRD
                     GCQGLEQFEQFKAELYGRRARAVLSLLTTPMLWQYVARSTTIKLRNLSTRRFA"
     gene            complement(1428..2930)
                     /locus_tag="VIO83_08470"
     CDS             complement(1428..2930)
                     /locus_tag="VIO83_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY8331342.1"
                     /translation="MANYVGQAVTTLVGIVFVPFYLRYLGAEAYGLVGFFVVLQLWLG
                     LLDVGLSPMLNREVARARADANGFTDFRRILRSVELIFAGIALATGLAMTLASPLLAA
                     HWFKVKSLDPAQVSQCLMLMGWMIGLRFFASIYRTALNGMEAQVSLNAINVGFAVAKA
                     VGSLAVLRYVSNTPLAFFLVQLLLGLLEPIVLAQRSYRVLPPTTERIGLAFAKAQLIT
                     AMPFGLSIAYTSILWLLLSQLDRLLLSGLLPLDEYGYFSLVMALTSAVTLAASPLSSA
                     LIPRLTYLHSKGEIDQLIRLYRNATQLTAVLMIPLTGMVALFGKELMYAWTGNLAAST
                     WASNIIFWYMLGNTFLTFTSYQYYLQYAYGDLALNVKINTLQAIIGAPLITLAAYRFG
                     ATGTAIVWCLLQAATFVVSPPLVQRKLAPGLYRPWMRQDLLPVATGAVLVLAVLSLLS
                     GDLIARFNRLEIFLALSVMGVCVVAGAAAGSSLLRSYLARMTHLAPTGHQ"
BASE COUNT          610 a         1012 c         1200 g          517 t
ORIGIN      
        1 cgccggcctg gagttcatcc gcatcgccgg cccgttcacc gtgctggccg agcacctgga
       61 gcagaccgtg gcgcgcgagg gctttgccga cttcaacgcc cagggcaaca ccgtctacgg
      121 cagctgggtg gtgaccggcg aatcgcgccc ctacaccggc acgctgggca acgtcacgcc
      181 caagggtccg gccggcgcgc tggaactgct gctgcgctac agcgacctgg acctcaacga
      241 tgccgggctg gaaggcggct accagcatga ctggaccgtg ggtgcgaact actacttcac
      301 cagccacttc aaggtgcagg tcaactacat caaggccaac agccgccgtg ccggggtgga
      361 ggtggacccc aacgcattcg ggatccgcgc gcaggtgatg ttctgaccgg cgccggcgtg
      421 ccgcgcgcga tgcgctaggc gaaacggcgc gtggacaggt tgcgcagctt gatggtcgtg
      481 gagcgggcca cgtactgcca cagcatcggc gtggtgagca ggctgagcac cgcgcgcgcg
      541 cgccggccgt agagctcggc cttgaactgt tcgaactgct caaggccctg gcagccgtcc
      601 ctgcagcgga tcagcgcctg cctcatctgc ggggtcagcg cgtgggcgta ggtgcgcaac
      661 aggtggtcca cgttctgtat gaccggctgc atgttgaagc gcgtgcgcga atgcggcgag
      721 cgggtgttgg agacgtcgct gtgtgacagc gtgtagacgt agtaagcctg cggcacgatg
      781 ctggccctgg cgccttccag caggcaacgg atgatgatgt cgaaatcctc gccaaagcgg
      841 caggcgggat cgtagcggag ctggtgctcg gtcaggaacg cgcgacggat gatcggcttg
      901 atcagcggga aataggcggc gcggctgccg gacaggatct cgcgcaggct cgggacgttg
      961 ctggatccct tgaggaagga cagcgcggtc aatggctggg ggtgctggct ttcattgaag
     1021 gccagcaggc cgacctgccg gtccagatat gaatcatgca gcacctggtt gtcggccacg
     1081 atatccagct gctgttcctc ggcgtgccgg atcaggtgtt ggagccgttg cggcagcatg
     1141 atgtcgtcgg catcgaggaa ggcgatgtaa cgcccgcggg cctgggcgat gccggtgttg
     1201 cgcgccacgg ccgcgccacc gttgcagtcc tgctcgaaca gccggatgtc ctggtcgtgg
     1261 acctgcaagg ccttgacggt ggccaccgta tcgtcggtgg aggcgtcgtc gacgaccagg
     1321 atttccaggt ctttcaaggt ctgctgccgg atcgaggcaa tgctgcggcc gatgaatccg
     1381 gccgcgttgt aggcaggcac cacgattgag gcgatgggat ccatgggtta ttgatgtcct
     1441 gtgggggcca gatgggtcat gcgggccagg taggagcgca gcagcgaact gcccgccgcc
     1501 gcgccggcca ccacgcacac gcccatgaca ctcagggcca ggaagatctc gagccggttg
     1561 aagcgggcga tgaggtcacc cgagagcagg gacagcaccg ccagcaccag caccgcacct
     1621 gtcgccacgg gcagcaggtc ctgtcgcatc cacggccggt acaggcccgg cgccagcttg
     1681 cgctggacca gcggcggaga caccacgaag gtcgccgcct gcagcaggca ccagacgatc
     1741 gccgtgccgg tggcgccgaa acggtaggcg gccagggtga tcagcggggc gccgatgatc
     1801 gcctgcagcg tgttgatctt gacgttcagc gccaggtccc cgtacgcgta ctgcaggtag
     1861 tactggtagg aggtgaaggt caggaaggta ttgcccagca tgtaccagaa gatgatgttc
     1921 gaggcccagg tggacgcggc gaggttgccg gtccaggcgt acatcagttc cttgccgaac
     1981 agcgccacca tgccggtgag cgggatcatc agtacggcgg tgagttgggt ggcattgcgg
     2041 tagagccgga tcagctggtc aatctcgccc ttggaatgaa ggtaggtcag gcgtgggatg
     2101 agcgcgctgc tgagcggcga ggcggccagg gtcacagccg aggtcaacgc catcaccagc
     2161 gagaaatagc catattcgtc caggggcagc aggccggaga gcagcagccg atccagctgg
     2221 gacagcagca gccacaggat cgaggtgtag gcgatgctca ggccaaaggg catggcggtg
     2281 atcagctggg ccttggcgaa ggccaggccg atgcgttcgg tggttggcgg caagacgcgg
     2341 tagctgcgct gggccagcac gatgggctcc agcagtccca gcagtagctg gacaaggaag
     2401 aacgccagcg gcgtattgct cacatagcgc agcaccgcca gcgaaccgac cgccttggcc
     2461 acggcgaagc cgacgttgat cgcgttgagc gagacctggg cctccatccc gttcagtgcg
     2521 gtccggtaga tcgaggcgaa gaaccgcagg ccgatcatcc agcccatcag catcaggcac
     2581 tggctgacct gggccgggtc cagcgacttc accttgaacc agtgcgcggc cagcagcggg
     2641 ctggccaggg tcatggccag gccggtggcc agggcgatgc cggcgaagat cagttccacg
     2701 ctgcgcagga tccggcgaaa gtcggtgaag ccgttggcat cggcgcgcgc gcgggccact
     2761 tcccggttga gcatcggcga gaggccgacg tcaagcagcc ccagccagag ctgcaggacc
     2821 acgaagaagc cgaccaggcc gtaggcttca gcacccaggt accgcaggta gaacggaacg
     2881 aagacgatgc cgaccagggt ggtcaccgcc tggccaacgt aattggccaa ggcgttcgtg
     2941 cgaacggagg gagagcgggt catgttcgcc tggggaaggt tgggtgctgc gtccccaagc
     3001 gtaacgccaa ggaggggctt ttcaggacgc gccttgccag ctgccggatg aaccgtgctt
     3061 cagccaggtc gcggcggcgg cctgcacctg gggaccggca atcggggggg ccggacggtg
     3121 ccggcaagcg gtctccacgg ccacgcgcgc gcccgaaaca ggccgccttc ggcctgcccg
     3181 cagcggcccg cgcgttagag cgcgtagccg aactgctgcc ggaactggtc gttgaactgg
     3241 tcgaagtcga agcgctggtt ctgggtgccg gggtgctcga ccttgagcgc gcccatcagg
     3301 ttgcccatgc gaccgatggt cagccagtcg cagccgcgc
//
LOCUS       DATCUN010000218         6696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_197313, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000218 DATCUN010000000
VERSION     DATCUN010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197313"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VIO83_08475"
     CDS             <1..550
                     /locus_tag="VIO83_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
                     /protein_id="HEY8331343.1"
                     /translation="LQLECIGCGACIDACDSIMDKMGYERGLVRYTSERELAGGKTRW
                     LRPRLVGYAAALLLMFGAFGWALSVRPMVQLDVTKDRGLFRENSEGQIENIYRLKVIN
                     KTQQPHLYAVSLVEPGPFVLQGERELQLAPGEILDVAVSVALTAERAPDSATPLRFAV
                     EDLDQPEVRVTAKSTFVSPLNR"
     gene            632..2041
                     /gene="mapR"
                     /locus_tag="VIO83_08480"
     CDS             632..2041
                     /gene="mapR"
                     /locus_tag="VIO83_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983343.1"
                     /note="MapR regulates genes involved in Pseudomonas
                     quinolone signal (PQS) production and anthranilate
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator MpaR"
                     /protein_id="HEY8331344.1"
                     /translation="MKRYEKFADEIAELIRTGVLAPGQKVPSVRHASRTYGVSPSTVF
                     QAYYLLESRGLILARARSGYFVRELAQRPLSEPGISAREAQTSVVDVSELVFSVLDSL
                     KDPDTVPFGSAFPSTDLFPLARLARSMAHGVRELSPRGVIADMTEGNADLRRQIALRY
                     MVSGVMLPLEELVITTGAMEALNLCLQCVTRPGDLVAIEAPAFYATLQVLERLELKAV
                     EIPVHPREGIDLASLEASLQQLPIKACWFMSSLQNPLGASMGEDKKRALYQLLQRHQV
                     PLIEDDVYAELYFGSQPPKPVKSFDREGLVMHCGSFSKCLAPGYRVGWVAGGRYAERI
                     RRLKLMTTISPSVPAQAAIADYLQHGGYDRHLRRLRHALETQQASMLASAARHFPAST
                     RVTRPSGGYFLWFEFPEQVDSLQLFQLALAQGISLAPGPIFSATRRFGNCARLNYGHP
                     WDAQSERAMEVLGRIIASF"
     gene            2159..3586
                     /gene="ccoN"
                     /locus_tag="VIO83_08485"
     CDS             2159..3586
                     /gene="ccoN"
                     /locus_tag="VIO83_08485"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003193164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEY8331345.1"
                     /translation="MSTTISEQAYDYRVVRQFAVMTVVWGVVGMAMGVFIASQLVWPA
                     LNFDLEWTSFGRLRPLHTSLVIFAFGGCGLFATSYYTVQRTCHTRLFGGPLIAFTFWG
                     WQAVIAIMLVSLPLGYTTTKEYAEIEFTGAVVLAIVWVAYAVVFFGTVMKRKTKHIYV
                     GNWFFGAFIVATAMLHIVNHLAIPVSWFKSYSIYSGATDAMVQWWYGHNAVGFFLTTG
                     FLGMMYYFVPKQADRPVYSYRLSIVHFWPLITLYIWAGPHHLHYTALPDWAQTLGMVM
                     SIVLLAPSWGGMINGMMTLSGSWHKLRTDPILRFLVVSLAFYGMSTFEGPMMAIKTVN
                     ALSHYTDWTIGHVHAGALGWVAMITFGSLYHLIPKVFGREQMHSIALINLHFWLATIG
                     TVLYIASMWVNGIMQGLMWRATNVDGTLTYSFVEALEASHAGFLVRLIGGLFFLTGML
                     LMAWNTWRTVRLADPVASQAHAKFA"
     gene            3638..3871
                     /locus_tag="VIO83_08490"
     CDS             3638..3871
                     /locus_tag="VIO83_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEY8331346.1"
                     /translation="MANELWLLTCVTLFISAVQLCLSGRSQRSLDEATMLPFADDPEV
                     AEQMEQETGRSRTGCACPGSCRGRCEDWQSFTA"
     gene            complement(3963..4844)
                     /locus_tag="VIO83_08495"
     CDS             complement(3963..4844)
                     /locus_tag="VIO83_08495"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY8331347.1"
                     /translation="MSNKVLFRPDEAAALMQAGKAVLIDIRDTESYQAGHIVGAVNVP
                     DVFSYLAQTSPEGLQALQAQFAELFGNAGLDGEKVAIVYEDALNTRYGGSCRGFWLLQ
                     YLGYPKVGILDGGLRAWKAFGGTVDNATVTPAAACFPVLPQAELLVTTEQMLAALADP
                     AIKLLDNRDKDEWLGESSSPYGKDFAPRKGRIPGAVWLEWYELMDTRKPVAAFKSASH
                     VRALAAERGLTPDDEIIIYCFKGARAANTYVAMKLAGFDKLRIYLGSWNEWSRDERLP
                     IETGLRKGAVRLLPQVV"
     gene            complement(4912..5361)
                     /gene="cynS"
                     /locus_tag="VIO83_08500"
     CDS             complement(4912..5361)
                     /gene="cynS"
                     /locus_tag="VIO83_08500"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HEY8331348.1"
                     /translation="MISSREEVTKLILAAKVSKGLKWSHVAEAIGLSKEWTTAGCLGQ
                     MTFDKPQAETLGKLFDLPDEAVAWLQIVPYKGSLPSAVPTDPLIYRWYEIVSIYGTTI
                     KELIHEEFGDGIMSAIDFSMDIQRQADPKGDRVNVVLSGKFLPYKSY"
     gene            complement(5445..6002)
                     /locus_tag="VIO83_08505"
     CDS             complement(5445..6002)
                     /locus_tag="VIO83_08505"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY8331349.1"
                     /translation="MSAATATCPTTVKTYLKPIDRDGLMAFAERGRNNPASRGTNKVH
                     SVMQGMYRSLSYVGNHEAVVVDEPLHLFGEDTAPAPGEIVLSGLGGCLAVGITAVATW
                     KQVKLTKLEIFLEGDIGNPAAWGAGGAEMQPEQMGFQAIRVKVLVEGDASREELDEIV
                     KHANFYSPVANTMRNPVPFSIGLAD"
     gene            6368..>6696
                     /locus_tag="VIO83_08510"
     CDS             6368..>6696
                     /locus_tag="VIO83_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8331350.1"
                     /translation="MVSKNTICLWYDGTAADAAKFYAETFPDSAVGAIVRAPGDYPAG
                     KQGDVLVVEFTVLGIPCMGLNGGPGIKHNEAFSFQVATDDQAETDRLWNAIVGNGGQE
                     SECGWCK"
BASE COUNT         1122 a         2240 c         2094 g         1240 t
ORIGIN      
        1 actgcagctg gagtgcatcg gctgcggtgc ctgcatcgac gcctgcgaca gcatcatgga
       61 caagatgggc tatgagcgcg gcctggtgcg ctacacctcc gagcgcgagc tggccggcgg
      121 caagacccgc tggctgcgcc ctcgcctggt cggctacgcc gccgcactgc tgctgatgtt
      181 cggcgccttc ggctgggcgc tgagcgtgcg cccgatggtg caactggacg tgaccaagga
      241 ccgcggcctg ttccgcgaga acagcgaggg acagatcgag aacatctatc gcctcaaggt
      301 gatcaacaag acccagcagc cgcacctcta cgccgtctct ctggtcgagc ctggtccctt
      361 cgtgctgcag ggcgagcgcg aactgcagct ggcgcccggg gaaatcctcg acgtcgcggt
      421 gagcgtggcg ctgaccgccg agcgcgcccc ggatagcgcc acgccgttgc gcttcgcggt
      481 ggaggatctg gaccagcccg aggtgcgcgt cacggctaag agcacgttcg tttcaccgct
      541 caatcgctga tcgcccagga aatattccca cgccatgaac agccggaact cgatagactg
      601 ccatgcatcc accccgctca acccggacaa gatgaagcgc tacgagaagt ttgccgacga
      661 gatcgccgag ctgatccgca ccggcgtgct ggcccccggg cagaaggtgc cctcggtgcg
      721 ccacgccagc cgcacctatg gggtcagccc gtcgacggtg ttccaggctt actacctgct
      781 ggaaagccgc ggcctgatcc tggcgcgggc gcgctcgggc tacttcgtcc gcgaactggc
      841 ccagcgcccg ctgtccgaac cggggatcag cgcccgcgag gcacagacca gcgtggtcga
      901 tgtcagcgag ttggtgttct cggtgctcga ctcgctgaag gacccggaca ccgtgccctt
      961 cggttcggcc ttccccagca ccgacctgtt ccccctcgcc cgcctggcgc gctccatggc
     1021 ccacggcgtg cgcgagctgt caccccgtgg ggtgatcgcc gacatgaccg agggcaacgc
     1081 cgacctgcgc cggcagatcg ccctgcgcta catggtcagc ggggtgatgc tgccgctgga
     1141 ggagttggtg atcaccaccg gcgccatgga ggcgctcaac ctgtgcctgc agtgcgtcac
     1201 ccggccgggc gatctggtgg ccatcgaggc gccggcgttc tacgccaccc tgcaggtgct
     1261 ggaacgcctg gaactcaagg cggtggaaat ccccgtgcac ccgcgcgagg gcatcgacct
     1321 ggccagcctg gaggccagtc tccagcagtt gccgatcaag gcctgctggt tcatgagcag
     1381 cctgcagaac ccgctgggcg cgagcatggg cgaggacaag aagcgggcgc tgtaccagct
     1441 gctgcagcgc catcaggtgc cgctgatcga ggacgacgtg tacgccgagc tgtacttcgg
     1501 cagccagcca cccaagccgg tgaagagctt cgaccgcgag ggcctggtga tgcactgcgg
     1561 ctcgttctcc aagtgcctgg caccgggcta ccgggtcggc tgggtggctg gcgggcgcta
     1621 cgccgagcgg atccgccggc tcaagctgat gaccaccatc tcgccgtcgg tgccggcgca
     1681 ggcggccatt gccgactacc tgcagcacgg cggctacgac cgtcatctgc gccggctgcg
     1741 ccacgccctg gaaacccagc aggcgtcgat gctcgcctcg gcggcgcggc acttcccggc
     1801 cagcacgcgg gtgacccggc ccagcggcgg ctatttcctc tggttcgagt tccccgaaca
     1861 ggtcgactcg ctgcagctgt tccagctcgc cctggcccaa ggcatcagcc tggcgccggg
     1921 gccgatcttc tcggccaccc ggcgcttcgg caactgcgcg cggctcaact acggccatcc
     1981 gtgggacgcg cagagcgagc gcgccatgga agtgctcggg cgcatcatcg cctcattctg
     2041 atccggtcta attgcgccga tctgagcctg ttttgcgcag aacccaagcc ttacagtgcc
     2101 ctcgcgcact cctctacgga gcgctcgcca tggacactgt ctgcagaaag gtttcgatat
     2161 gagcacaacg atttccgaac aggcgtacga ctaccgggtc gtgcgccagt ttgccgtgat
     2221 gaccgtggtc tggggcgtcg tcggcatggc catgggggtg ttcatcgcct cgcaactggt
     2281 ctggcccgcc ctcaacttcg acctcgaatg gaccagcttc gggcgcctgc gcccactgca
     2341 caccagcctg gtgatcttcg ccttcggcgg ttgcgggctg ttcgccacct cctactacac
     2401 ggtacagcgc acctgccata cccgcctgtt cggcggcccg ctgatcgcct tcaccttctg
     2461 gggctggcag gcggtgatcg ccatcatgct ggtcagcctg ccgctgggct acaccaccac
     2521 caaggaatac gccgagatcg agttcaccgg cgcggtggtg ctggccatcg tctgggtcgc
     2581 ctacgccgtg gtgttcttcg gcacggtaat gaagcgcaag accaagcaca tctacgtcgg
     2641 caactggttc ttcggcgcct tcatcgtcgc cacggcgatg ctgcacatcg tcaaccacct
     2701 ggccatcccg gtcagctggt tcaagtccta ttcgatctac tccggcgcca ccgacgccat
     2761 ggtgcagtgg tggtacgggc acaacgcggt gggcttcttc ctgaccaccg gcttcctcgg
     2821 catgatgtac tacttcgtgc ccaagcaggc cgaccgcccg gtgtattcct atcggctgtc
     2881 catcgtgcac ttctggccgc tgatcaccct gtacatctgg gccggcccgc accacctgca
     2941 ctacaccgcc ctgcccgact gggcgcagac cctcggcatg gtgatgtcga tcgtcctgct
     3001 ggcgccgagc tggggcggca tgatcaacgg catgatgacc ctgtccggct cctggcataa
     3061 gttgcgcacc gacccgatcc tgcgcttcct ggtggtgtca ctggccttct acggcatgtc
     3121 caccttcgag ggcccgatga tggcgatcaa gaccgtcaac gccctctccc actacaccga
     3181 ctggaccatc ggccacgtgc acgccggggc gctgggctgg gtggcgatga tcaccttcgg
     3241 ctcgctgtac cacctgattc ccaaggtgtt cggccgcgag cagatgcaca gcatcgccct
     3301 gatcaacctg cacttctggc tggccaccat cggtaccgtg ctgtacatcg cctcgatgtg
     3361 ggtcaacggc atcatgcagg gcctgatgtg gcgggctacc aacgtggatg gcaccctgac
     3421 ctactccttc gtcgaggcgc tggaagccag ccacgccggc ttcctcgtgc gtctgatcgg
     3481 cggcctgttc ttcctcaccg gcatgctgct gatggcctgg aacacctggc ggaccgtgcg
     3541 cctggccgac cctgtcgcct ctcaggctca tgcgaagttc gcatgacctt tcgcccggtg
     3601 ctcccgcgcc gggctttttt attcccggag aaacaccatg gccaacgaac tctggctgct
     3661 gacctgtgtg accctgttca tcagcgccgt gcagctctgc ctgtccggcc gaagccagcg
     3721 cagtctggac gaggcgacta tgctgccctt tgcggacgac ccggaagtgg ctgagcaaat
     3781 ggagcaagag accgggcgca gccgcacggg ctgcgcctgc ccgggctctt gccgtggtcg
     3841 ctgtgaggac tggcagagct tcacggcctg atgccaggcg cagctcgcag aggacgccat
     3901 acctccgaac tgaaacaaat gcggcgagcc ctgaccagga ctcgccccga ccgcgccacc
     3961 agtcagacca cctgcggcaa cagacgcacg gctcctttgc gcaggccggt ttcgatgggc
     4021 aggcgctcgt cacgcgacca ttcgttccag gaaccgagat agatgcgcag cttgtcgaag
     4081 ccagccagct tcatggcaac gtaggtattg gccgcccgcg cgcccttgaa gcaatagatg
     4141 atgatctcgt catcgggcgt caggcctcgc tcggccgcca aggcacgtac gtgactagcc
     4201 gacttgaatg ccgcgaccgg cttgcgggta tccatcagct cgtaccactc cagccagacc
     4261 gcaccgggga tgcgaccctt gcggggtgcg aagtccttgc cgtagggcga cgagctttcc
     4321 cccagccatt catccttgtc gcgattgtcg agcagcttga tcgctggatc ggccagtgcg
     4381 gccagcatct gctccgtggt caccagcagc tcagcctgcg gcagaacggg gaagcacgcc
     4441 gctgcggggg ttaccgtcgc gttgtcgacc gtgccgccga atgccttcca ggcccgcaga
     4501 ccaccatcga ggatgccgac cttcgggtag ccgaggtact gcaacagcca gaaaccgcgg
     4561 cacgatccgc catagcgggt attcagcgca tcctcgtaga cgatggcgac cttctcgcca
     4621 tccaggccgg cgttgccgaa cagctcggcg aattgcgcct gcaaggcctg cagcccctcg
     4681 gggctggttt gcgccaggta ggaaaagaca tccggcacat tgaccgcgcc gacgatgtgc
     4741 ccggcctgat aactctcggt gtcgcggatg tcgatcagca cggccttgcc tgcttgcatc
     4801 aaggctgccg cttcgtcggg cctgaaaagc actttgttgc tcatggttcc atcctctccg
     4861 gtttgctccg gccagcctga agctggccga gagcatgggc tgcagcagga atcagtagct
     4921 cttgtagggc aggaacttgc cggacaggac cacgttgacg cggtcgccct tcgggtcggc
     4981 ctggcgctgg atatccatgg agaagtcgat ggcgctcatg atgccgtcgc cgaactcctc
     5041 gtggatcagc tccttgatgg tggtgccgta gatgctgacg atctcgtacc agcggtagat
     5101 cagcgggtcg gtgggcacgg cgctcggcag cgagcccttg tacggcacga tctgcagcca
     5161 ggccaccgcc tcgtcgggca gatcgaacag cttgccgagg gtttccgcct ggggcttgtc
     5221 gaaggtcatt tggcccaggc agccggccgt ggtccactcc ttggacaggc cgatggcctc
     5281 ggccacatgg ctccacttca ggcctttgct gaccttggcg gccaggatca gtttggtcac
     5341 ttcttcgcgg ctggaaatca tgatgcgttc ctcattggtt cggtgtccgg gcagcgccgc
     5401 actggcgact gcccgcaaag tggtcgcagg gcgttgcgtc agtctcagtc ggccaggccg
     5461 atgctgaacg ggaccgggtt gcgcatggtg ttggccaccg gcgagtagaa gttggcgtgc
     5521 ttgacgatct cgtccagctc ctcgcggctc gcatcgccct ccaccagtac cttcacgcga
     5581 atcgcctgga agcccatctg ctcaggctgc atctcggcac caccggcacc ccaggccgcc
     5641 gggttgccga tatcgccctc gaggaagatt tccagcttgg tcagcttcac ctgcttccag
     5701 gtcgccaccg cggtgatgcc cacggccagg caaccgccca gaccggagag gacgatctcg
     5761 cctggggccg gcgcggtgtc ctcgccgaac aggtgcagcg gctcgtcgac caccacggct
     5821 tcgtggttgc cgacatagct caggctgcgg tacatgccct gcatgacgga gtggaccttg
     5881 ttggtgccac gggaggccgg gttgttgcgg cccctctcgg cgaaggccat caacccgtcg
     5941 cgatcgatcg gcttcaggta ggtcttcacg gtggtcgggc aagtggcggt tgctgcagac
     6001 atggcattct ccatcgggtt ttgatcgagc agttgggctg ctcttttgga ctcgaatgct
     6061 ctgttgcagc aggtgtgcca agtcacccca tgccgccaga atctgatttt tattcctttt
     6121 aaatcaaatg cttatccaat aaaggcgcac cctcccacaa ccttgttttc agcaaacctt
     6181 gggtatagtc ggaagctgac attgtcgaca ttgacgacaa tgtcagctcg cgaattggct
     6241 caggtcgctg gtgggtctac ccgggcggcg cccatgtccg cgatggcgcg gaggctcacc
     6301 agaagcgcca gttgcccaaa agctcggcta gccttcgtga atcaccgctc acaagtggga
     6361 gacaagcatg gtcagcaaga acacgatctg cctctggtac gacggcaccg cggcggacgc
     6421 agcgaagttc tacgccgaga cctttccgga cagcgcagtg ggagccatcg tgcgcgcgcc
     6481 cggcgactat cccgccggca agcagggcga tgtcctggtg gtcgagttca ccgtgctggg
     6541 cattccttgc atgggcctca acggcggccc ggggatcaag cacaacgagg cgttctcatt
     6601 ccaggttgcc accgacgacc aagccgaaac cgaccgcctg tggaacgcga tcgttggcaa
     6661 cgggggccag gaaagcgaat gcggctggtg caagga
//
LOCUS       DATCUN010000219         5057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_9449, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000219 DATCUN010000000
VERSION     DATCUN010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9449"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VIO83_08515"
     CDS             complement(<1..207)
                     /locus_tag="VIO83_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331351.1"
                     /translation="MPAGVDNGRRHGLAREIRMPLRYLFKQLLLPPGGLFVLLLLAWW
                     WRARRPRLALACGVLGLGGLWLLSL"
     gene            423..3044
                     /gene="leuS"
                     /locus_tag="VIO83_08520"
     CDS             423..3044
                     /gene="leuS"
                     /locus_tag="VIO83_08520"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY8331352.1"
                     /translation="MHEQYQPREIEAAAHSHWDAHQSFEVSEQPGKDTFYCLSMFPYP
                     SGKLHMGHVRNYTIGDVIARHQRMQGKNVLQPMGWDAFGMPAENAAMQNQVAPAAWTY
                     ANIDYMKTQLKSLGLAIDWSREVTTCKPDYYRWEQWLFTRLFEKGVIYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALVEKREIPMYYFKITAYADELLQSLDDLDGWPEQVKT
                     MQRNWIGKSRGMEVSFPYDAASIGSEGVLKVFTTRPDTLMGATYVAVAAEHPLASIAL
                     RQLPADQAAELQAFVDECKRGGVAEADIATQEKKGLATGLFVQHPLTGEKLPVWVANY
                     VLMNYGEGAVMAVPAHDERDFEFAHKYALPIKTVIRTSAGDQTPAPWQDAYAEHGQLI
                     NSAEFDGLDFAGAFDALEVALQKKGLGQARTQFRLRDWGISRQRYWGCPIPIIHCASC
                     GDVPVPEDQLPVVLPEDVVPDGAGSPLARMPEFYECSCPKCGGAARRETDTMDTFVES
                     SWYYARYASPQFEGGMVDKAAANHWLPVDQYIGGIEHAILHLLYARFFHKLMRDEGLV
                     DSDEPFTNLLTQGMVIAETYYRLQDNGAKLWFNPADVELELDAKGKPLSAKLKSDGLP
                     VEIGGIEKMSKSKNNGVDPQTMIEQYGADTCRLFMMFASPPDMSLEWSDSGVEGASRF
                     LRRVWRLAHAHVSTGLPGALDKTALSDAQKDVRRAIHLAIRQASQDIGQHHKFNTAIA
                     QVMTLMNVLEKAPQSEAQDRALLQEGLEAVALLLAPITPHISHALWQQLGHAEAIIDA
                     TWPQVDEAALVQDSLTLVVQVNGKLRGEIQVAASASREDVEAAARANENVLRFTEGTT
                     IRKVIVVPGKLVNIVAN"
     gene            3120..3716
                     /gene="lptE"
                     /locus_tag="VIO83_08525"
     CDS             3120..3716
                     /gene="lptE"
                     /locus_tag="VIO83_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY8331353.1"
                     /translation="MKRSLTGLLLVMLTTTLVACGFQLRGVGSNDFALKELDLSARNA
                     YGQTVKDVRRQLEGSGVRVHDGAPYELVLTGEGEQQRTASYTSSARSAEYQLTTSLDY
                     EIRSGNLSLLDDKLEVQRVYVHDSNNLIGSDQESQQLRTEMRRELVQQLLMRLQQLTP
                     ARLGELQEKAQARAEAAAKASAAARQTAPAPALPLLQR"
     gene            3757..4794
                     /gene="holA"
                     /locus_tag="VIO83_08530"
     CDS             3757..4794
                     /gene="holA"
                     /locus_tag="VIO83_08530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY8331354.1"
                     /translation="MKLNPAQLAKHLQGPLAPVYAVSGDDPLLCQEACDAIRAVCRAR
                     DFSERQVFHVDASFDWNLLREAGASLSLFADKRLLELRLPGGKPGDEGTRVLLEYLAR
                     PAEDTVLLLSLPRLDGKTQKSKWAKALIDGDNCQFVQIWPVDAAQLPQWIRQRLAQVG
                     LAASAEAVDLLVARIEGNLLAAVQEIEKLRLLVEGNQLDVQTVQASVADSARFDLFGL
                     LDAALAGDATHALRMLEGLRGEGVEAPVILWGLARELRLLATLAQQHGQGQPLERLIA
                     QAKPPIFGKRQALVGRAVQRQPASRWNQLLRDAQLIDAQIKGQAPGDAWSGLAQLALQ
                     IAGVRLNLPSE"
     gene            4892..>5057
                     /gene="arfA"
                     /locus_tag="VIO83_08535"
     CDS             4892..>5057
                     /gene="arfA"
                     /locus_tag="VIO83_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014592531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072344 - rescue of stalled ribosome
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue factor ArfA"
                     /protein_id="HEY8331355.1"
                     /translation="MAKPRKPGPNKAKSLVAQPLFRCRQERPAKGKGSYQREAFRFTD
                     REASSFPGQEK"
BASE COUNT          923 a         1836 c         1621 g          677 t
ORIGIN      
        1 caggctcagc agccacagtc cgcccaggcc gagcacacca caggccagcg ccagccgcgg
       61 ccgccgcgcc cgccaccacc aggccagcag cagcaacaca aacagcccgc ccggcggcag
      121 cagcaattgc ttgaacaggt aacgcagcgg cattctgatc tcccttgcca gcccatggcg
      181 gcggccgttg tcgacaccgg ccggcatcat atagcgaata cccgctcgcc tcgcgggcag
      241 ggcgccgcct tcgtcgcgcg ccagccctcc ggcaggagcc cgcatgaccg cccctccgcc
      301 gcctgccgca cgaccgagca gcggccccgc ccgcctgcgg cgatcagggt ttcccctgat
      361 ccgcgggggt catgtatgct tagccacttt ggtcaactcc ctttttccag tccggcagag
      421 ccatgcacga acagtatcag ccccgcgaaa tcgaagccgc cgcgcactcc cactgggacg
      481 cgcaccagtc ctttgaagta agtgaacagc caggcaagga caccttctac tgcctgtcga
      541 tgttccccta cccgagcggc aagctgcaca tgggccacgt gcgcaactac accatcggcg
      601 acgtgatcgc gcgccaccag cgcatgcagg gcaagaacgt gctgcagccg atgggctggg
      661 acgccttcgg catgccggcg gaaaacgctg caatgcagaa ccaggtcgcc cccgccgcct
      721 ggacctacgc caacatcgac tacatgaaga cccagctgaa gagcctgggc ctggccatcg
      781 actggtcgcg cgaagtcacc acctgcaagc cggactacta ccgctgggaa cagtggctgt
      841 tcacccgcct gttcgagaag ggcgtgatct accgcaagaa cggcacggtc aactgggacc
      901 cggtggacca gaccgtgctg gccaacgagc aggtgatcga cggtcgcggc tggcgctccg
      961 gcgcgctggt cgagaagcgc gagatcccga tgtactactt caagatcacc gcctatgcgg
     1021 atgagctgct gcagtccctc gacgatctgg acggctggcc cgagcaggtc aagaccatgc
     1081 agcgcaactg gatcggcaag tcgcgcggca tggaagtgag cttcccctac gacgccgcca
     1141 gcatcggcag cgagggcgtg ctgaaggtgt tcaccacccg cccggacacc ctgatgggcg
     1201 ccacctacgt ggccgtggcc gccgaacacc cgctggccag cattgccctg cgccagctgc
     1261 cggccgacca ggccgccgaa ctgcaggcct tcgtcgacga gtgcaagcgc ggcggcgtgg
     1321 ccgaagcgga catcgccacc caggagaaga agggcctggc caccggcctg ttcgtccagc
     1381 acccgctgac cggcgagaaa ctgccggtgt gggtcgccaa ctacgtgctg atgaactacg
     1441 gcgaaggcgc ggtgatggcc gtgccggccc acgacgagcg cgacttcgag ttcgcccaca
     1501 agtacgccct gccgatcaag accgtgatcc gcaccagcgc cggcgaccag accccggcgc
     1561 cgtggcagga cgcctacgcc gagcacggcc agctgatcaa ctccgccgag ttcgacggcc
     1621 tcgacttcgc cggcgccttc gacgccctcg aagtggccct gcagaagaaa ggcctcggcc
     1681 aggcgcgcac ccagttccgc ctgcgcgact ggggcatcag ccgccagcgc tactggggct
     1741 gcccgatccc gatcatccac tgcgccagct gcggcgacgt gccggtgccg gaagaccagc
     1801 tgccggtggt gctgcccgag gacgtggtgc cggacggcgc cggcagcccg ctggcgcgca
     1861 tgcccgaatt ctacgaatgc agctgcccga aatgcggcgg cgcggcgcgg cgcgaaaccg
     1921 acaccatgga cactttcgtc gaatcgtcct ggtactacgc gcgctacgcc tcgccgcagt
     1981 tcgagggcgg catggtcgac aaggccgcag ccaaccactg gctgccggtc gaccagtaca
     2041 tcggcggcat cgagcacgcc atcctgcacc tgctctacgc gcgcttcttc cacaagctga
     2101 tgcgcgacga gggcctggtc gacagcgacg agccgttcac caacctgctg acccagggca
     2161 tggtgatcgc cgagacctac taccgcctgc aggataatgg cgccaagctg tggttcaacc
     2221 cggccgacgt cgagctcgag ctggacgcca agggcaagcc gctgtccgcc aagctgaaga
     2281 gcgacggcct gccggtggaa atcggcggca tcgagaagat gtccaagtcg aagaacaacg
     2341 gcgtcgaccc gcagaccatg atcgagcagt acggcgccga cacctgccgc ctgttcatga
     2401 tgttcgcctc gccgccggac atgagcctgg agtggtccga ctccggcgtc gagggcgcca
     2461 gccgcttcct gcgccgcgtc tggcgtctgg cccacgccca cgtcagcacc ggcctgccgg
     2521 gcgcgctgga caagaccgcg ctgagcgatg cccagaagga cgtgcgccgc gccatccacc
     2581 tggccatccg ccaagccagc caggatatcg gccagcacca caagttcaac accgccatcg
     2641 cccaggtgat gaccctgatg aacgtgctgg agaaggcgcc gcagagcgag gcccaggacc
     2701 gcgccctgct gcaggaaggc ctcgaggccg tggccctgct gctggcaccg atcaccccgc
     2761 acatcagcca cgcgctgtgg cagcagttgg gccatgccga ggcgatcatc gacgccacct
     2821 ggccgcaggt cgacgaagcc gcgctggtgc aggacagcct gaccctggtg gtgcaggtca
     2881 acggcaagct gcgcggcgaa atccaggtcg ccgcttcggc ctcccgcgag gacgtcgaag
     2941 ccgctgcccg cgccaacgag aacgtgctgc gcttcaccga aggcaccacc atccgcaagg
     3001 tgatcgtggt ccccggcaag ctggtcaaca tcgttgccaa ctgagtgtcg ccacgcgacg
     3061 caagccatcc aacccgccgc ccgcccatcg cgggcggcgc tgcacaagag ggaagactga
     3121 tgaaacggag cctgaccggc ctgctgctgg tgatgctgac cactacgctc gtcgcctgcg
     3181 gctttcagct gcgcggcgtc ggcagcaacg atttcgccct gaaggaactc gacctcagcg
     3241 cccgcaacgc ctacggccag accgtcaagg acgtgcgtcg ccagctggaa ggcagcggcg
     3301 tgcgcgtgca tgacggcgcg ccctacgagc tggtcctgac cggcgaaggc gagcagcagc
     3361 gcaccgccag ctacaccagc tcggcgcgca gcgccgagta ccagctgacc accagcctcg
     3421 actacgagat ccgcagtggc aatctgtccc tgctcgacga caagctggaa gtgcagcggg
     3481 tctacgtgca cgactccaac aacctgatcg gctccgacca ggagtcccag cagctgcgca
     3541 ccgagatgcg ccgcgaactg gtgcagcagc tgctcatgcg cctgcaacag ctgacgccgg
     3601 cgcgcctcgg cgagctgcag gaaaaggccc aggcccgtgc cgaggccgcc gccaaggcca
     3661 gcgcagccgc ccgccagacc gcgccggcac cggctctgcc gctgctgcag cgctgatcag
     3721 ccagacgggg ccgcagggcc ccgttggctc ctgccgatga aactcaatcc cgcccaactg
     3781 gccaagcacc tgcagggccc gctcgcgccg gtgtacgcgg tcagcggcga cgacccgctg
     3841 ctgtgccagg aagcctgcga cgccatccgt gctgtctgtc gcgcccgcga cttcagcgaa
     3901 cgccaggtat tccacgtcga cgccagcttc gactggaacc tgctgcgcga ggccggcgca
     3961 agcctgtcgc tgttcgccga caagcgcctg ctggaactgc gcctgcccgg cggcaagccc
     4021 ggcgacgagg gcacccgcgt gctgctcgag tacctcgccc gcccggccga ggacaccgtg
     4081 ctactgctca gcctgccgcg gctcgacggc aagacccaga agagcaagtg ggccaaggcg
     4141 ctgatcgacg gcgacaactg ccagttcgtg cagatctggc cggtggacgc cgcgcagctg
     4201 ccgcagtgga tccgccagcg cctggcccag gtcggcctgg ccgccagcgc cgaggcggtg
     4261 gacctgctgg tggcgcgcat cgagggcaac ctgctggcgg cggtgcagga aatcgagaaa
     4321 ctgcgcctgc tggtcgaggg caaccagctc gatgtgcaga cggtgcaggc cagcgtggcc
     4381 gacagtgcgc gcttcgacct gttcggcctg ctcgacgcgg cgctggccgg cgacgccacg
     4441 cacgccctgc gcatgcttga gggcctgcgc ggcgagggcg tggaagcgcc ggtgatcctc
     4501 tggggtctgg cccgcgaact gcgcctgctc gccactctcg cccagcagca cggccagggc
     4561 cagccgctgg agcggctgat cgcccaggcc aagccgccga tcttcggcaa gcgccaggcc
     4621 ctggtcggcc gcgccgtgca gcgccagccg gcgagccgct ggaaccagct gctgcgcgat
     4681 gcccagctga tcgacgcgca gatcaagggc caagcgcccg gcgatgcctg gagcgggctg
     4741 gcgcaactgg ccctgcagat cgccggcgtg cgcctcaacc tgcccagcga gtgattccgc
     4801 tcgttttttg cacaacgcca ctggacattt tctgtacagc gaccgaagat gcgcgcgcct
     4861 gcacgctggc aggtccgcac gaggaaaggt catggccaag ccacgcaaac ccggcccgaa
     4921 caaggccaaa tccctggttg cacaaccgct gttccgctgc cgccaggaac gcccggccaa
     4981 gggcaagggc agctaccaac gcgaagcctt ccggttcacc gaccgggagg cttcttcctt
     5041 tccgggccag gaaaaag
//
LOCUS       DATCUN010000220         3893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_179232, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000220 DATCUN010000000
VERSION     DATCUN010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179232"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VIO83_08540"
     CDS             complement(<1..694)
                     /locus_tag="VIO83_08540"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEY8331356.1"
                     /translation="MARINLLPWRAERRKQRQRDFYGMLGFAAIGGVLLSLLIWFYYD
                     RQVSGQGERNAFLQTEIAKVKEQNKEIDRLDRQRDRLLARKKVIEELQAKRSQMVHLF
                     DALVRTIPDGVVLTALKQEGDILTLQGRTQSNARVSAYMRNLETSGWMSKPELSVIEA
                     KKPEPGKDGPTTLTDISALPYMFEVKVTLPAQSEPVNGVNPDGSVVVPADAAAAPADP
                     AAAPVAPLSPGAA"
     gene            complement(694..1752)
                     /locus_tag="VIO83_08545"
     CDS             complement(694..1752)
                     /locus_tag="VIO83_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY8331357.1"
                     /translation="MGLIPKSQSPLIGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLP
                     PNAVVEKNIVEVEAVGEAIRRAVNRSGTKARHAAAAVAGSAVITKLIPMPADLDENDM
                     EAQVELEAVNYIPYPIEEVNLDFEVLGAIPNNPEMVQVLLAASRSENVELRQSALELG
                     GLTARVMDVEAFAVENAFALVASELPIASDGVVALVDIGATMTTLNVLRGGRSLYSRE
                     QVFGGKQLTDEVMRRYGLTYEEAGLAKRQGGLPESYEMEVLEPFKEATVQQISRLLQF
                     FYAGSEFNRVDHIVLAGGCAALAGLPEMVEEQLGVATVVANPLAQMTLGPKVQAHALA
                     QDAPALMIATGLALRSFD"
     gene            1977..>3893
                     /locus_tag="VIO83_08550"
     CDS             1977..>3893
                     /locus_tag="VIO83_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY8331358.1"
                     /translation="MTRFRRWLRWLLVAILVLGLIGAAVAGGLYYAISSKLPDVQTLR
                     DIEMQEPMYVYASDGKLMAVFGESRRVPITMKEVPERLKQAFLATEDARFYEHGGVDY
                     KGIGRAVWLLATTNDKRVPGGSTITQQVARQFFLSSEYSYTRKLAEILLARKIESELS
                     KDEIFELYLNKSFFGNRAYGVAAAAEFYYGKKLGDLDLDEMASLAGIPKFPSSGNPIS
                     NPERARERRDFYVLQRMASLGFISQAEADAAKAVPMHASAHEPPVQVEAPYVAELVRQ
                     EMIARFGGDVVNKGYHVTTTINAELQTAANIAVRDGLRVYDHRHGWHGVEQHFEVPAD
                     ADAKALAAKLVGIPAQSGLLPAIVASTAADGSATVVLANRSELVLPVAASRWVNKTPA
                     KLLTRGDLVRVRAGEKEGEWLIDQIPRGQSALVSLDAESGALKALVGGFSFSGNKFNR
                     ATQARRQPGSSFKPFIYAAAFDKGFNPASIVLDAPVVFRDRRGKTWSPQNDGGGFRGP
                     MRLREALVQSRNLVSVRLLDGMGVDYARKYISEFGFAEAELPPNLSMSLGTASVTPLS
                     VARGYAVFANGGSRVDTWVIDQVTDRDGVVVFKENPAMACRDCAGTSGGAPVSQVVDG
                     FNFGAPAAPATAAAP"
BASE COUNT          638 a         1327 c         1253 g          675 t
ORIGIN      
        1 gtgcagcacc gggcgacagc ggggccaccg gcgcggcggc cggatcagcc ggggcggcgg
       61 cggcatcggc cggcaccacc acgctgccat cggggttgac gccattgacc ggctcgctct
      121 gcgccggcag ggtgaccttg acctcgaaca tgtacggcaa cgcgctgatg tcggtcaggg
      181 tggtgggacc gtccttgccg ggctcgggct tcttggcctc gatcaccgac agctccggct
      241 tactcatcca gcccgacgtt tccaggttgc gcatgtaggc gctgacccgg gcgttggact
      301 gggtgcgccc ctgcagggtg aggatgtcac cttcctgctt gagcgcggtc agcaccaccc
      361 catcgggaat ggtgcgcacc agcgcgtcga acaggtggac catctgcgag cgcttggcct
      421 gcagttcctc gatcaccttc ttgcgcgcca gcagccggtc acgctggcga tccaggcggt
      481 cgatctcctt gttctgctcc ttgaccttgg cgatctcggt ctgcaggaag gcattgcgct
      541 cgccctgccc gctgacctgc cggtcgtagt agaaccagat caacaacgac agcaggaccc
      601 cgccgatggc ggcgaagccg agcatgccgt agaagtcgcg ctggcgttgc ttgcgccgct
      661 cggcgcgcca cggcaataga ttgattcttg ccatcagtca aagctcctca gcgccagacc
      721 ggtggcaatc atcagcgccg gggcatcctg ggccagggca tgcgcctgga ccttgggacc
      781 cagggtcatc tgtgccagcg ggttggccac cacggtggcc acgcccagct gttcttccac
      841 catctccggc aacccggcca gcgccgcgca accgccggcc agcacgatgt ggtccacgcg
      901 gttgaattca ctaccggcat agaagaactg gagcaggcgg ctgatctgct ggaccgtggc
      961 ttccttgaac ggctccagca cttccatctc gtagctttcc ggcagcccgc cctggcgctt
     1021 ggccaagccg gcttcctcgt aggtgaggcc gtagcggcgc atcacttcgt cggtgagctg
     1081 cttgccgccg aacacctgtt cgcggctgta caggctacgc ccgccccgca ggacgttcag
     1141 ggtggtcatg gtggcgccga tgtccaccag ggcgaccacg ccatcactgg cgatcggcag
     1201 ttcgctggcg accagggcaa aggcgttctc gaccgcgaag gcctccacgt ccatgacccg
     1261 ggcggtcagc ccgccgagct ccagcgccga ttggcgcagc tccacgttct ccgaccgcga
     1321 cgccgccaac agcacctgga ccatctccgg gttgttggga atggcgccca gcacctcgaa
     1381 atcgaggttc acttcctcga tcgggtacgg gatgtagttg accgcttcga gctcgacctg
     1441 ggcttccatg tcgttctcgt ccagatcagc cggcatcggg atcagcttgg tgatcaccgc
     1501 cgagccggcg acggctgcgg cagcatgccg ggccttcgta ccggaacggt tcacggcgcg
     1561 gcggatagcc tcacccacgg cctccacttc cacgatgttc ttttccacga ccgcattcgg
     1621 cggaagtggt tccacagcgt aatgttccac gcggaaacga ttgccactgc gggaaagctg
     1681 caagagcttt actgcagtcg agctgatgtc gacgccaata agcggcgact ggctttttgg
     1741 gataagcccc acggtttctc ccctgccgac gggcacttgg acgcataacc tgcgcccgcg
     1801 cattaataac gttttttttg caaaccgcaa ccatcacgca gtgaaacgtg cactaccgca
     1861 cgtttcacgg ctccccagac ggtcgtcgat ctgcgctccc cgggaacaac cccagccgtt
     1921 cccttgcgta atctatactc tgcggccacg aaattcgcaa tcggaatctg tcactgatga
     1981 cacgctttcg ccgctggctg cgctggttgc tggtcgccat cctggtcctg ggcctgatcg
     2041 gcgccgccgt cgcaggtggc ctgtactacg ccatttcctc caagctcccg gacgtgcaga
     2101 ccctgcgcga catcgagatg caggagccca tgtacgtgta tgccagcgat ggcaagctga
     2161 tggccgtctt cggcgagtcg cggcgcgtac cgatcaccat gaaggaggtc ccggaacgtc
     2221 tcaagcaggc cttcctggcc accgaggacg cccgcttcta cgagcatggc ggggtggact
     2281 acaagggcat cggccgcgcg gtctggctgc tggccaccac caacgacaag cgcgtgccgg
     2341 gtgggtccac catcacccag caggtcgccc gccagttctt cctcagctcc gaatacagct
     2401 acacgcgcaa gctggccgag atcctgctgg cgcgcaagat cgagtccgag ctgagcaagg
     2461 acgagatctt cgagctgtac ctgaacaaga gtttcttcgg caaccgcgcc tacggcgtgg
     2521 ccgctgcggc cgagttctac tacggcaaga agctgggcga tctggacctg gacgagatgg
     2581 cctcgctggc cggcatcccc aagttcccgt cctcgggcaa cccgatcagc aaccccgagc
     2641 gggcccgcga gcgccgcgac ttctatgtgc tgcagcgcat ggccagcctg ggcttcatca
     2701 gccaggccga ggccgacgcc gccaaggcgg tgccgatgca cgccagcgcg catgagccac
     2761 cggtgcaggt ggaagccccc tacgtggccg agctggtccg ccaggaaatg atcgcccgct
     2821 tcggcggcga cgtggtcaac aagggctacc acgtgaccac cacgatcaat gccgagttgc
     2881 agacggcggc caacatcgcc gtgcgcgatg gcctgcgcgt gtacgaccac cggcatggct
     2941 ggcatggcgt ggagcagcac ttcgaggtgc cggccgatgc cgatgccaag gcgctggcgg
     3001 ccaagctggt cggcattccg gcccagtccg ggctgctgcc ggcgatcgtg gcatccactg
     3061 ccgccgatgg cagcgccacc gtggtgctgg ccaaccgcag cgagctggtg ctgcccgtcg
     3121 ccgccagccg ctgggtcaac aagaccccgg ccaagctgct gacccgaggc gacctggtgc
     3181 gcgtgcgcgc cggcgagaag gaaggcgagt ggctgatcga ccagatcccg cgcggccagt
     3241 ccgcgctggt ctcgctggat gctgaaagtg gcgccctgaa ggcactggtg ggcggcttca
     3301 gcttctccgg caacaagttc aaccgtgcca cccaggcgcg ccgccagccc ggctcgagct
     3361 tcaagccgtt catctatgcg gccgcgttcg acaagggctt caaccccgcc tcgatcgtgc
     3421 tggatgcccc ggtggtgttc cgcgaccgcc gcggcaagac ctggtcgccg cagaacgacg
     3481 gcggcggctt ccgtgggccg atgcgcctgc gtgaggcgct ggtgcagtcg cgcaacctgg
     3541 tctcggtgcg cctgttggac ggcatgggcg tggactacgc acgcaagtac atcagcgagt
     3601 tcggctttgc cgaggccgag ctgccgccca acctgtcgat gtcgctgggt accgcctcgg
     3661 tgaccccgtt gtcggtggcg cgcggctatg ccgtgttcgc caatggcggt tcgcgggtgg
     3721 acacctgggt gatcgaccag gtgaccgacc gtgacggggt ggtggtgttc aaggaaaacc
     3781 cggccatggc ctgccgcgac tgcgccggta ccagcggcgg cgcgccggtc agccaggtgg
     3841 tggacggctt caactttggc gcaccggctg ccccggccac cgccgcggcg ccc
//
LOCUS       DATCUN010000221         6635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_197423, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000221 DATCUN010000000
VERSION     DATCUN010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197423"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VIO83_08555"
     CDS             complement(<1..1012)
                     /locus_tag="VIO83_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010211748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta (1-6) glucans synthase"
                     /protein_id="HEY8331359.1"
                     /translation="MTSQPRSILIYCCTLLATLLALLGLWYGLGREQPLPDPQLANGK
                     LQCASYSPFDHDQSPFNKPLNISRERVEADVALLAQRFNCLRTYSVTGLEYLPDIARR
                     HGIKLMIGAWVSRAPKATRKEIELLVKTANAHPDVVTAVIVGNEALLRREITAKRLSA
                     LIREVKSRVSQPVTYADVWEFWLRHPEMAPAVDFITIHLLPYWEDDPTGIDGALAEVK
                     QVHEKFERLYAPKPIFVGETGWPSEGRQREDALPSRVNEARFIRGVIDMAEQNGWQYN
                     LIEAFDQPWKRISEGTVGGYWGLFDAYRADKGVLAGPVSNLPLWRNWLGVSALLLAAG
                     LLL"
     gene            complement(1123..1347)
                     /locus_tag="VIO83_08560"
     CDS             complement(1123..1347)
                     /locus_tag="VIO83_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEY8331360.1"
                     /translation="MLKTLTFTLMHFCIAFGVTYALTGSVAVGGLVATVEPLCNAVGF
                     FFHDKLWKRLEKHATADNEPARQSWQHRHA"
     gene            1621..1938
                     /locus_tag="VIO83_08565"
     CDS             1621..1938
                     /locus_tag="VIO83_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEY8331361.1"
                     /translation="MNNNENVYRQIYANPRFQELVAKRGRFAWMLSIIMLAAYFAFIL
                     LIAFDPKVLGIPLGPDTVTTWGIPAGLGLIFLAIALTGVYVQRANGEFDRINQEILNE
                     AQK"
     gene            1938..3596
                     /locus_tag="VIO83_08570"
     CDS             1938..3596
                     /locus_tag="VIO83_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEY8331362.1"
                     /translation="MIARFLTAATLLAASPMLLAGEALTGEVQKQATNWTAITMFVIF
                     IAFTMGITKWAAKRNTSTADYYTAGGSITGFQNGLAIAGDYMSAASFLGISALVFTSG
                     YDGLIYSIGFLVGWPLILFLMAERLRNLGKFTFSDVASYRLGQTQIRILSALGSLTVV
                     AFYLIAQMVGAGKLIQLLFGLDYYVAVILVGVLMVCYVLFGGMLATTWVQIIKAVLLL
                     SGASFMALMVMKGVNFDFGTLFSEAVKIHTKGEAIMSPGGLVTDPVSAISLGLALMFG
                     TAGLPHILMRFFTVSDAKEARKSVFYATGFIGYFYILTFIIGFGAILLVSTNPEYKDA
                     TGAIIGGTNMVAIHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYAC
                     VIKKGKAKEEDEMRVTKITTLTLGVVAILLGIAFEKQNIAFMVGLAFSIAASCNFPVL
                     FLSMYWKNLTTRGALFGGALGLFTALVLTILSPTVWVQVLGNAQAIFPYSYPALFSVT
                     VAFIGIWFFSITDKSKMASDERERFFPQFVRSQTGLGATGAVAH"
     gene            3749..4177
                     /locus_tag="VIO83_08575"
     CDS             3749..4177
                     /locus_tag="VIO83_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331363.1"
                     /translation="MDPESIVYGCIRDCPDGEPSARRQRRAHNRQMLDELPGLGGLPL
                     LSRQMFSFCRFEGEGLYQTQVIHFGASYRAVEYEWATWLQQFETLLGRLYWSSAVVHL
                     DTELRGFHTFRWESEQGFHQPGEGVLRMRCAWEHEGSLRG"
     gene            complement(4194..4424)
                     /locus_tag="VIO83_08580"
     CDS             complement(4194..4424)
                     /locus_tag="VIO83_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HEY8331364.1"
                     /translation="MLIPHALLEPETLTRLIEDFVTREGTDNGDDTPLTTRVARVRHA
                     LERGEAVIVFEPDSQQCQLMLRREVPVEWLTD"
     gene            complement(4501..4770)
                     /locus_tag="VIO83_08585"
     CDS             complement(4501..4770)
                     /locus_tag="VIO83_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HEY8331365.1"
                     /translation="MTDTPQAPQQDAMLKRLARIEGQVRGLQAMIRRGEDCEAIAQQF
                     AAARKALDRAYQELLACLLEEAVLDPQQDSRATLARVRSIFTKYT"
     gene            complement(4767..5321)
                     /locus_tag="VIO83_08590"
     CDS             complement(4767..5321)
                     /locus_tag="VIO83_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003303979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEY8331366.1"
                     /translation="MRIATLVLLATLGSQAVAADTQQLAAEGAALIPPFQQQLLETVK
                     TAMQSGGPVKAVEACQLLAPQITSQHSKAPWKVGRTALKLRNPANAPDAWERRVLEQF
                     AARAAAGEPIAELKQAEIVDGEFRLMKAIPTGEPCLACHGQQIKPELAAVIDQRYPQD
                     QARGFALGELRGAFTLRRSLTDQP"
     gene            complement(5460..>6635)
                     /gene="mnmC"
                     /locus_tag="VIO83_08595"
     CDS             complement(5460..>6635)
                     /gene="mnmC"
                     /locus_tag="VIO83_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEY8331367.1"
                     /translation="GGLAGCATASSLAERGWQVTLIERHAELAMEASGNPQGVLYLKL
                     SAHGTALSKLVVSGFGHTRRQLERLSAGADWSACGVLQLAFDAKEAERQARLAGAFPA
                     DLLRSVDASEASQLAGLPLEHGGLWYAEAGWVHPPALCRQLASHPNIRVLPSLEALDL
                     QRVDGQWQASQDGRLLAAAPVAVLCTASEVRNFAATAHLKLKRIRGQITRLPATAASR
                     SMQTVLCAEGYVAPPRGDEHTLGASFNFQRDDLDLCPDEHADNLGMLRQISPALGEAL
                     DIAHLDPAQLQGRAAFRSTSPDYLPLIGPVVDARAFNAAYAGLAKDASRRIDTPAPWL
                     EGLYVNCGHGSRGLITAPLSGELLAAWLDDEALPLPRAVAEACHPSRFLLRELIRGR"
BASE COUNT         1011 a         2109 c         2270 g         1245 t
ORIGIN      
        1 cgagcagcag gccggcagcc agcagcaggg cgctcacgcc cagccagttg cgccacagcg
       61 gcagattgga caccggcccg gcgagcacgc ccttgtcggc gcgataggcg tcgaacaggc
      121 cccagtagcc gccgaccgtg ccctcgctga tgcgcttcca cggctggtcg aaggcttcga
      181 tcaggttgta ttgccagccg ttctgctcgg ccatgtcgat gaccccgcgg atgaagcgtg
      241 cctcgttgac tcggctgggc agggcgtcct cgcgctggcg gccttcgctc ggccagccgg
      301 tttcgcccac gaagatcggt ttgggggcgt acaggcgctc gaacttctcg tgcacctgct
      361 tgacctcggc gagggcgccg tcgatgccgg tcggatcgtc ttcccagtag ggcagcaggt
      421 ggatggtgat gaagtcgacc gccggggcca tctccgggtg gcgcagccag aactcccaga
      481 catcggcgta ggtcactggc tggctgaccc gcgacttcac ttcgcggatc agcgcgctca
      541 ggcgcttggc ggtgatttcc cggcgcagca gcgcctcgtt gccgacgatc actgcggtca
      601 ccacgtccgg atgggcgttg gcggtcttca ccagcagttc gatttccttg cgcgtggcct
      661 tgggggcgcg gctgacccag gcgccgatca tcagcttgat gccgtggcgg cgggcgatgt
      721 cgggcaggta ttcgagaccg gtcaccgaat aggtgcgcag gcagttgaag cgctgggcga
      781 gcagggccac gtcggcctcc acgcgctcgc ggctgatgtt cagcggcttg ttgaacggcg
      841 actggtcatg gtcgaacggg ctgtaggagg cgcattgcag tttgccgttg gccagttgcg
      901 ggtcgggcag gggctgttcg cggcccaggc cataccagag cccgagcagg gcgagcaggg
      961 tggcgagcag ggtgcagcag tagatcagaa tggagcgggg ctgggaggtc atggcggcgc
     1021 aggttcaagg tcgaggccgc ggatattaaa gagcggaccc cgtgcgcgcc agaggcgcgc
     1081 tcattctgtc atggggctgg ccgaagatag ccccggcagg actcaggcgt gccggtgttg
     1141 ccagctctgc cgcgcgggct cgttgtcagc cgttgcatgc ttttccaggc gtttccacag
     1201 cttgtcgtgg aagaagaagc ccacggcatt gcacagcggc tcgacggtcg ctaccagccc
     1261 gccgacggcc acgctgccgg tcagcgcgta ggtcacgccg aaggcaatgc agaagtgcat
     1321 cagggtgaag gtcagggtct tgagcatggc acgcctccat gtactcgcac gtgagaatgg
     1381 ttttctcttg tgggaattct ggccggctca tgcccggtag tgaaattatc gacgttgatg
     1441 gtgaggatag tgccggtcta ttgccattct gctgccggtt cgccagagcc ctggcagtgc
     1501 tgcgggcggt caaggacggg catgcgggga ctactaaagt cgtctggtat tcgccatgcc
     1561 gccccctaaa gtaacgtaca tgccgcccat tgggaggctc gacagacgtg gagactaata
     1621 atgaataaca atgaaaacgt gtaccggcag atctatgcca acccacgctt ccaggagctg
     1681 gtcgccaagc gcggccgctt tgcctggatg ctctcgatca tcatgctggc agcctacttc
     1741 gccttcatcc tgctgatcgc cttcgatccc aaggtgctgg gcattccgct cggtccggat
     1801 accgtgacca cctggggcat cccggctggt ctgggcctga tcttcttggc catcgcactg
     1861 accggtgtct acgtgcagcg cgccaacggc gagttcgacc gcatcaacca ggaaatcctg
     1921 aacgaggccc aaaaataatg attgctcgtt tcctgaccgc tgccaccttg ctggcagcct
     1981 ccccgatgtt gctggcgggc gaagccctga ccggcgaagt ccaaaaacag gcgaccaact
     2041 ggaccgccat caccatgttc gtgatcttca tcgccttcac gatgggtatc accaagtggg
     2101 cggccaagcg caacacctcc accgccgact actacaccgc cggcggcagc atcaccggct
     2161 tccagaacgg cctggcgatc gccggtgact acatgtccgc ggcgtccttc ctgggtattt
     2221 ccgccctggt attcaccagc ggttacgacg gcctgatcta ctccatcggc ttcctggtcg
     2281 gctggccgct gatcctgttc ctgatggctg aacgcctgcg caacctgggc aagttcacct
     2341 tctccgacgt ggcctcctac cgcctcggcc agacccagat ccgcatcctg tcggctctcg
     2401 gctcgctgac cgtggtggcc ttctacctga tcgcccagat ggtcggtgcc ggcaagctga
     2461 tccagctgct gttcggtctc gactactacg tcgcggtgat cctggtcggc gtgctgatgg
     2521 tctgctacgt gctgttcggc ggcatgctgg ccaccacctg ggtgcagatc atcaaggcag
     2581 tcctgctgct gtccggtgcc agcttcatgg cgctgatggt gatgaagggt gtgaacttcg
     2641 acttcggtac cctgttctcc gaagccgtga agatccacac caagggcgaa gccatcatga
     2701 gcccgggcgg cctggtgacc gatccggtct ctgcgatctc cctgggtctg gcgctgatgt
     2761 tcggtactgc tggtctgccg cacatcctga tgcgcttctt caccgtgtct gacgccaagg
     2821 aagcccgcaa gagcgtgttc tacgctaccg gcttcatcgg ctacttctac atcctgacct
     2881 tcatcatcgg cttcggcgcg atcctgctgg tcagcaccaa tccggagtac aaggacgcta
     2941 ctggcgccat catcggcggc accaacatgg tggctatcca cctggccaac gctgtcggcg
     3001 gcaacctgtt cctcggcttc atctccgccg tagctttcgc caccatcctg gcggtggttg
     3061 ccggtctgac cctggcgggt gcctcggccg tgtcccacga cctgtacgcc tgcgtgatca
     3121 agaagggcaa ggccaaggaa gaggacgaga tgcgcgtgac caagatcact accctgactc
     3181 tgggtgtggt cgcgatcctg ctcggtatcg ccttcgagaa gcagaacatc gccttcatgg
     3241 tgggtctggc cttctccatc gccgccagct gcaacttccc ggtcctgttc ctctccatgt
     3301 actggaagaa cctgaccact cgtggcgcgc tgttcggtgg tgctctgggt ctgttcaccg
     3361 cgctggtgtt gaccatcctc agcccgaccg tatgggtcca ggtgctgggc aacgcccagg
     3421 cgatcttccc gtactcctac ccggcgctgt tctcggtcac cgtcgccttc attggtatct
     3481 ggttcttctc gatcaccgac aagtccaaga tggccagcga cgagcgcgag cgtttcttcc
     3541 cgcagtttgt gcgttcgcag actggcctgg gtgctaccgg tgccgtagcg cactaagcaa
     3601 cagggtcaat cgaccgtcga aaaaggggca gcctgcgggc tgcccctttt tttgtgtcga
     3661 ctgccggcgc cgcgagtctt gcggcagttc ctttccccgc gctgcctgct gtgatagaag
     3721 gcgggcctgt cctttcagga gtgtccgcgt ggaccccgaa tccatcgttt atggctgcat
     3781 ccgcgactgt ccggacggtg agccgtcagc ccggcgccag cgccgcgcgc acaaccggca
     3841 gatgctcgac gagctgccgg ggctgggtgg gttgccgctg ctcagtcggc agatgttctc
     3901 gttctgccgt ttcgagggag aagggctgta ccagacccag gtgattcatt tcggcgccag
     3961 ctaccgcgca gtggagtacg agtgggcgac ctggctgcag cagttcgaga cgctgctcgg
     4021 gcgcctgtac tggtcgagtg cggtggtgca tctggatacc gaactgcgcg gctttcacac
     4081 gtttcgctgg gaaagcgagc agggcttcca tcagccgggc gagggcgtgc tgcgcatgcg
     4141 ttgcgcctgg gagcacgagg gtagcttgcg cggctgagca gcgcgccgga cggtcagtca
     4201 gtcagccatt cgaccggcac ttcgcggcgc agcatcagct ggcactgctg gctgtccggt
     4261 tcgaacacga tcaccgcctc gccgcgctcg agggcgtggc ggacccgtgc gacgcgggtg
     4321 gtcagcggcg tgtcgtcgcc attgtcggtg ccctcgcggg tgacgaagtc ctcgatcagg
     4381 cgggtcaggg tctcgggctc gagcagcgcg tggggaatca gcatgagggg atctcggcaa
     4441 aacgggtggc gatcctggca tccgtgccgc tgtctggcta tccctgtcgg cgtcgtcgac
     4501 tcaggtgtac ttggtgaaga tgctgcgcac ccgcgccagg gtggcgcggc tgtcctgctg
     4561 cggatcgagc acggcttcct ccagcaggca ggcgagcagt tcctggtagg cgcgatccag
     4621 cgccttgcgg gcggcggcga actgctgggc gatggcctcg cagtcctcgc cgcggcggat
     4681 catcgcctgc agaccgcgga cctggccttc gatgcgcgcc agacgcttga gcatggcgtc
     4741 ctgctggggg gcttgcggcg tatcggtcat ggctggtcgg tcagcgagcg gcgcaatgtg
     4801 aaagccccgc gcaattcgcc gagggcgaag ccgcgggcct gatcctgtgg atagcgctgg
     4861 tcgatcaccg cggccagctc cggcttgatc tgctggccat ggcaggctag gcagggttct
     4921 ccggtgggga tggccttcat caggcggaat tcgccatcga cgatctcggc ctgcttgagc
     4981 tcggcgatcg gctcgccggc ggcggcgcgg gcggcgaact gctccagcac ccggcgctcc
     5041 caggcatccg gggcattggc cggattgcgc agcttcagcg cggtgcgccc gactttccag
     5101 ggcgccttgc tgtgctggct ggtgatttgc ggggcgagca gttggcaggc ttcgaccgcc
     5161 ttgaccgggc cgccgctctg catggcggtc ttgacggtct ccagcagctg ctgctggaac
     5221 ggcggaatca gcgcggcgcc ttcggcggcg agctgctggg tgtcggcggc gacagcctgg
     5281 ctgccgagcg tggcgagcag gacgagagtg gcgatgcgca tgacagagct cctgtcgaag
     5341 tttcctcgga tacctgtggg agtatctgca ggcgttgccg ccaaagcaat cccccgactg
     5401 acgcaggggc atgccggcac tccgcctgcc gcgccgcaga gggtgccgcg gggctggggt
     5461 cagcgcccgc ggatcagctc gcgcagcagg aaacggctgg ggtggcaggc ctcggcgacg
     5521 gcgcgcggca gcggcagcgc ttcgtcgtcc agccaggccg ccagcagttc gccggacagc
     5581 ggcgcagtga tcagtccgcg cgagccatgc ccgcagttga cgtacagccc ctcgagccag
     5641 ggtgccgggg tgtcgatgcg ccgactggcg tccttggcca ggccggcgta ggcggcgttg
     5701 aacgcacgcg catccaccac cgggccgatc agcggcaggt agtccgggct ggtgctgcga
     5761 aaggcggcgc ggccttgcag ctgcgcgggg tcgaggtgtg cgatgtccaa cgcttcgccc
     5821 agtgcgggcg agatctgccg gagcatgccg aggttgtcgg catgctcgtc cgggcacagg
     5881 tcaagatcgt cacgctggaa attgaagctg gcgcccaggg tgtgctcatc gccgcgcggc
     5941 ggggcgacgt agccctcggc gcacagcacg gtctgcatgc ttcggctggc ggcggtggcg
     6001 ggcaggcggg tgatctggcc gcggatgcgc ttgagcttga ggtgcgcggt ggcggcgaag
     6061 ttgcggacct cgctggcggt gcacagtacg gcgaccggtg cggcggccag caggcggccg
     6121 tcctggctgg cctgccactg gccgtcgacg cgctgcaggt cgagcgcctc gaggctgggc
     6181 agcacgcgga tgttcgggtg gctggccagc tgtcggcaca gcgccggcgg gtgcacccag
     6241 ccggcttcgg cgtaccacag gccaccgtgt tccagcggca ggccggccag ttgcgaggct
     6301 tcgctggcgt ccaccgaacg cagcaggtcg gcggggaagg caccggccag gcgcgcctgg
     6361 cgctcggctt ccttggcatc gaaggccagt tgcagcacgc cacaggcgct ccagtcggca
     6421 ccggcggaca gacgctcgag ctggcgccgg gtgtggccga aaccgctcac caccagcttc
     6481 gacaaggcgg tgccatgggc cgacagcttg aggtagagca cgccctgcgg attgcccgag
     6541 gcctccatgg ccagctcggc gtggcgctcg atcagggtga cctgccagcc gcgctcggcc
     6601 aggctgctgg ccgtggcgca gccggccagg ccgcc
//
LOCUS       DATCUN010000222         5197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_47582, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000222 DATCUN010000000
VERSION     DATCUN010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47582"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..680
                     /locus_tag="VIO83_08600"
     CDS             351..680
                     /locus_tag="VIO83_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331368.1"
                     /translation="MELNKAVLDCMQTLRRRLRDELGADIHLNQSDAVSALLGACERS
                     DSVRTRELGEELARLCAPQPAPAAQEERAQPLYRGQMPAAAEPVAPPAAPEERQVRIY
                     RGQRVYS"
     gene            958..1539
                     /locus_tag="VIO83_08605"
     CDS             958..1539
                     /locus_tag="VIO83_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEY8331369.1"
                     /translation="MAFELPPLPYEKNALEPHISTETLEYHHGKHHNAYVVNLNNLVP
                     GTEFEGKTLEEIVLTSSGGIFNNAAQVWNHTFYWNCLKPNGGGQPTGALADAINAAFG
                     SFDKFKEEFSKVSIGTFGSGWGWLVKKADGSLALASTIGAGNPLTSGDTPLLTCDVWE
                     HAYYIDYRNLRPKYVEAFWNLVNWDFVAANYAA"
     gene            1727..3811
                     /locus_tag="VIO83_08610"
     CDS             1727..3811
                     /locus_tag="VIO83_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009045342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8331370.1"
                     /translation="MSPYLRKSLSQRLFGQVLLAALLLGLLFISAQVAYSLQQTRQAI
                     RADGEQILDMLRAPSTQAAYQHDHGMAVQMLAGLLAHPGVRLASIRDGDGALLASQQR
                     APLAEAHNALSDWLFGREQHFAIRLSAAPPHPEDYGNLEVTLDTAYRANEFLAGARFM
                     LVSSLSGALLLGLALLLLFRWQLSRPLARLIEYLARINPERPAEHPLPLIKGHEDNEL
                     GLWINGTNRLLAAIARNSNRRREAEDNLLRMTQFDLLTGLPNRQLLQQQLEQILRDAN
                     RTQSRVAVLCLGLDDFKAVNEKYGYQTGDRLLVAVAERLRALDSRLGSLARLGGDQFA
                     LIQIAFEHTYEAAELAQQVLDALARPQHIDQHEISLCATLGITLFPEDGESTERLLQR
                     AEQTMTLAKQSARNHYQFYIASLDREIRRQRALEHDLRQALHNGQLELYYQPQVSNPG
                     HQAVAVEALLRWRHPQQGFIAPDQFIPLAEQNGLILEIGDWVLERACRQLHDWHAQGL
                     AGLRMAVNLSTAQLRSEKLPRQIVNLTQRYKLPPYSLELEVTETGLMADIASAKQQLL
                     SLRLAKVLLAIDDFGTGYSSLSYLKSLPLDKIKIDKSFVRDLTRDEDDATLVRTIIQL
                     AHNLNMKVIAEGVETAEQEAYLLAHGCSEGQGYLYCRPLPALEVTHYLFNATRRSPAA
                     VRHLEPAATPDD"
     gene            complement(3816..4718)
                     /locus_tag="VIO83_08615"
     CDS             complement(3816..4718)
                     /locus_tag="VIO83_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331371.1"
                     /translation="MVAVEPGLLVGLGMAAAACIGGGWWCLQRLAQARLLEDMPTSRV
                     RSAAQGYVELYGVLLPGPGEPLSAPLTGKPCLWWRYRIEEYRESGKSKSWRVLESASS
                     EGWLGFEDGSGQCLINPVGAEVRPARREVWHGERRHPRREDGHGLLGQLLGMGKRYRY
                     VEERLHAGEPLYALGEFRTSGGGRQGLDLAAAQAAVVREWKQDFRGLLARFDRDGSGD
                     LNDQEWRLVRLAAQLEAEDRHRAASCAPELNHLRQPQEALPFLLSCKGEDELARAFRW
                     QAAGGALLCLGGALAGTWLLAQGL"
     gene            complement(4718..>5197)
                     /locus_tag="VIO83_08620"
     CDS             complement(4718..>5197)
                     /locus_tag="VIO83_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003294644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY8331372.1"
                     /translation="GVGKAWSNIDVLLRQRHEELPKLVETCKQYMQHERTTLEAVIAA
                     RSAVASAREQGDVGALGRAESGLRAGLGQLFAVAENYPQLKANESFQHLQQRITGLEN
                     GIADRRELYNESVNLNNVRIEQFPDVLIARMFAFQAADLLQFSEAEKADVDLKPLFG"
BASE COUNT          918 a         1950 c         1588 g          741 t
ORIGIN      
        1 ccgcgcgcgc ggcggcggga gccggcatgg cacgactgcg cggcggcggc tcgtcgacag
       61 caaacgccgg ctcgcggtca tcggtcttca agctgggcag atcgtccagc tcgtccaggg
      121 aatcgttacg catgccaaca caacctgtga gtgcaaccgg aatggcgctc agtataccgc
      181 ctggccagcg cgacttcagg gctgcctcgc cgcggtgcaa caacgcaccg taacgaagcc
      241 gcagcgcggc tccgcgcgag cacaagccgc accgcgtcac ccggcaaagc tggtacgaag
      301 attgctccct cttcattcaa tactgcgagg ctgctcaagg gagagacccc atggaactga
      361 acaaggccgt gctcgattgc atgcagacac tgcgccgccg cctgcgcgat gaattgggcg
      421 cggacatcca cctcaaccag agcgatgccg tcagcgccct gctcggcgcc tgtgagcgca
      481 gcgactcggt gcgcacccgc gaactcggcg aggaactggc acgcctgtgt gcgccgcaac
      541 ccgcgccggc agcccaggag gaacgggcgc agccgctgta tcgcggccag atgcccgccg
      601 ccgcggaacc cgtcgccccg ccagccgctc ccgaagagcg gcaggtgcgc atctaccgcg
      661 gccagcgcgt ctacagctga tccgccagcg cggcggacag cgcccatcca cccccgcgcc
      721 gccgccaccg cgcccccctt tgcgacccgg cgcagcggcc gccccacaag agctgcgcag
      781 gggcctttcc cccacagatg ccgcgtggat aagccctggg gccagtgcta tgatccaaag
      841 ttcacagcgc cccaggggcc tggacagaac cgttgcaacg gccctgtcac accggtcgcg
      901 tgaaccggct tcgcgcacct tgcttgctca cacctgatga cgatacggag aatcaccatg
      961 gctttcgaac tgccgccgct gccttacgag aagaacgccc tcgagccgca catttccacc
     1021 gagaccctgg agtaccacca cggcaagcac cacaacgcct acgtggtcaa cctgaacaac
     1081 ctggtcccgg gcaccgagtt cgaaggcaag accctggaag agatcgttct gacctcctcc
     1141 ggcggcatct tcaacaacgc cgcccaggtg tggaaccaca ccttctactg gaactgcctg
     1201 aagccgaacg gcggtggcca gccgaccggt gccctggctg acgccatcaa cgccgccttc
     1261 ggttccttcg acaagttcaa ggaagagttc agcaaggttt ccatcggcac cttcggctcc
     1321 ggctggggct ggctggtgaa gaaggccgac ggttccctgg ccctggccag caccatcggc
     1381 gccggcaacc cgctgaccag cggcgacact ccgctgctga cctgcgacgt ctgggaacac
     1441 gcctactaca tcgactatcg caacctgcgt ccgaagtacg tcgaggcgtt ctggaacctg
     1501 gtcaactggg acttcgtggc tgccaactac gccgcctgag tcgaccccag ctgcacctga
     1561 agaagcccgg ccctgccggg cttcttcgtt tccgcccgcg gcctgccgcc gccgcgacac
     1621 gaccagcgtc gcgcattcgc cacgtcccct ttgacgccgc ccggccgatt cccaatactc
     1681 tgcaacgctt cacgagccga gaagcgccag acaaggaagc gtgccaatgt cgccatacct
     1741 ccggaagagt ctctcgcagc gactgttcgg ccaggttctg ttggcggccc tgctcctcgg
     1801 cctgctgttc atcagcgcac aggttgccta cagcctgcag cagacgcgcc aggccatccg
     1861 cgccgatggc gaacagattc tcgacatgct ccgtgcaccc tcgacccagg cggcctacca
     1921 gcacgaccac ggcatggccg tgcagatgct ggccggcctg ctcgcgcacc ccggggtacg
     1981 cctggccagc atccgcgacg gcgacggcgc actgctggcc agccagcaac gcgcccccct
     2041 ggccgaggcg cacaatgcgc tgagcgactg gctgttcggc cgcgaacagc acttcgccat
     2101 ccgcctgagc gccgcgcccc cacacccaga ggattacggc aacctcgaag tcaccctcga
     2161 caccgcctac cgggccaacg agttcctcgc cggcgcgcgc ttcatgctgg tcagcagcct
     2221 cagcggcgca ctactgctcg gcctggcgct gctcctgctg tttcgctggc agctgagtcg
     2281 gccgctggcg cgcctgatcg agtatctggc gcggatcaac cccgagcgtc cggccgaaca
     2341 cccgctgccc ctgatcaagg ggcacgaaga caacgaactc ggcctgtgga tcaacggcac
     2401 caaccggctg ctcgccgcca tcgcgcgcaa cagcaaccgg cgccgcgagg cggaggacaa
     2461 cctgctgcgc atgacccagt tcgacctgct caccgggctg cccaaccgtc aactgctgca
     2521 gcaacagctg gagcagatcc tccgcgatgc caaccgcacg cagagccggg tggcggtgct
     2581 ctgcctgggg ctcgacgact tcaaggcggt caacgagaag tacggctacc agaccggcga
     2641 ccgcctgctg gtggcggttg ccgagaggct gcgcgccctc gacagtcgcc tcggcagcct
     2701 ggcgcggctg ggcggcgacc agttcgcgct gatccagatc gccttcgagc acacctacga
     2761 ggccgccgaa ctggcccagc aggtactcga cgctctggcc cgaccgcagc acatcgacca
     2821 gcacgagatc agcctgtgcg ccaccctcgg catcaccctg ttccccgagg atggcgaaag
     2881 caccgagcgc ctgctgcagc gcgccgagca gaccatgacc ctggccaagc agagcgcgcg
     2941 caaccactac cagttctaca tcgccagcct cgaccgcgaa attcgccgcc agcgcgcgct
     3001 ggaacacgac ctgcgccagg cgctgcacaa cggccagctg gagctgtact accagccgca
     3061 ggtgagcaat cccggccacc aggcggtggc ggtcgaggcc ctgctgcgct ggcgtcatcc
     3121 gcagcagggc ttcattgcgc ccgaccagtt catcccgctg gccgagcaga acggtctgat
     3181 tctggagatc ggcgactggg tgctggagcg cgcctgccgc cagttgcacg actggcatgc
     3241 gcaggggcta gccgggctgc gcatggcggt caacctgtcc accgcgcaac tgcgcagcga
     3301 gaagctgccg cgccagatcg tcaacctgac ccagcgctac aagctgccgc cctacagcct
     3361 cgagctggag gtgaccgaga ccgggctgat ggcggatatc gccagcgcca agcaacaact
     3421 gctcagcctg cgcctggcca aggtgctgct ggcgatcgac gacttcggca ccggctattc
     3481 atcgctgagc tacctgaaga gcctgccgct ggacaagatc aagatcgaca agagcttcgt
     3541 gcgcgacctc acccgtgacg aggacgacgc caccctcgtg cgcaccatca tccagctggc
     3601 ccacaacctg aacatgaagg tgatcgccga aggcgtggaa accgccgaac aggaggccta
     3661 cctgctcgcc cacggctgca gcgaaggcca gggctacctg tactgccggc cgctgccggc
     3721 cctggaagtg acccactacc tgttcaacgc cacgcggcgc agtccagcgg cggtccgcca
     3781 tctggaaccg gccgccacac ccgacgactg aagccctaca gaccctgggc caacagccag
     3841 gtcccggcca gcgccccgcc cagacagagc agcgccccgc ccgccgcctg ccagcggaag
     3901 gcccgcgcca gctcgtcctc gcccttgcag gagaggagaa acggcagcgc ctcctgcggc
     3961 tggcgcaggt gattgagctc cggcgcgcag ctcgccgccc ggtgccgatc ctcggcttcc
     4021 agctgcgcgg ccaggcggac caggcgccac tcctggtcat tgagatcgcc gctgccatcg
     4081 cggtcgaagc gcgccagcag gccgcggaaa tcctgcttcc actcgcgcac caccgcggcc
     4141 tgcgccgcgg ccagatccag tccctgacgg ccgccgccgc tggtgcggaa ctcgcccaag
     4201 gcatacaacg gctcgccggc atgcaggcgc tcctcgacgt agcgatagcg cttgcccatg
     4261 cccagcagct gccccagcaa gccatggcca tcctcgcgac gtggatgacg acgctcgccg
     4321 tgccagactt cgcgccgggc cggacgcacc tcggcgccga ccgggttgat caggcactgc
     4381 ccggaaccgt cctcgaagcc cagccagccc tcgctgctgg ccgactccag cactcgccag
     4441 gacttgctct tgccgctctc gcggtactcc tcgatgcggt agcgccacca caggcacggc
     4501 ttgccggtca gaggcgcact caacggctcg cccggccccg gcagcagcac accgtacagc
     4561 tcgacgtagc cctgcgccgc cgagcggacc ctggaggtcg gcatgtcctc cagcagacgg
     4621 gcctgcgcca ggcgctgcag gcaccaccaa ccgccgccga tgcaggctgc agcggccatg
     4681 ccgaggccga ccagcaggcc cggttccacc gcgaccatca gccgaacagc ggcttgaggt
     4741 cgacgtcggc cttctccgcc tcgctgaact gcagcaggtc ggcggcctgg aaggcgaaca
     4801 tccgcgcaat cagcacgtcg gggaactgtt cgatgcgcac gttgttcagg ttgaccgact
     4861 cgttgtacag ctcgcggcgg tcggcgatgc cgttctccag cccggtgatg cgctgctgca
     4921 ggtgctggaa gctctcgttg gccttgagct gcgggtagtt ctccgccacc gcgaacagct
     4981 ggccgaggcc ggcgcgcaag ccgctctcgg cgcgaccgag ggcaccgacg tcgccctgct
     5041 cgcgggcgct ggccaccgca ctgcgcgcgg cgatcaccgc ctcgagggtg gtgcgctcgt
     5101 gctgcatgta ctgcttgcag gtttccacca gcttgggcag ctcctcatgg cgctggcgca
     5161 gcagcacgtc gatgttcgac cacgccttgc ccacccc
//
LOCUS       DATCUN010000223         4324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_122177, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000223 DATCUN010000000
VERSION     DATCUN010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122177"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..1880
                     /locus_tag="VIO83_08625"
     CDS             297..1880
                     /locus_tag="VIO83_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015371701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEY8331373.1"
                     /translation="MKYPLRPLVFWPTFLILLAAVIASYVDLTAFLAVSKQLNTVILQ
                     NFSWLFSAGSFAMLVLTAIVYFSPLGQVRIGGEGATPLLSKWRWFMVALCTTLAVGVL
                     FWTTAEPLYHLYSPPSSLPIEAGSAAAKSFALSTMFLHWTLTPYAIYLVPSLVFALVF
                     YNLRSHFSIGAMLQPLLGAERVKRYAGLIDTLAMFALVAGMASSLGTGALTLSGGLSQ
                     YIGGETTPLRLGLIIAVIVFTFVASAASGLQRGIVMLSSFNTWIMLALGAFVLVCGPT
                     LYIFSLGVESLGVYLDTFFSRSLSTGAASGDSWPQSWTVFYWSVWFAWAPVSALFLGK
                     IGRGYRVREFIQINMLYPALFTALWICIFSGSSLYFDALGDGSLNRVLNEQGVEHVLY
                     QMFQQLPASGLMIAFLLFVAFISFVTAADSSTDVIANLCSKGVTADSDLDGNPLLKIV
                     WGVIIGTVSWVMVAFVGIDGVKMLSNLGGLPGMILVLLASTSLLYWLKNPALLDVKAP
                     AVHVEPGLRGASPVADFAR"
     gene            1908..>4324
                     /locus_tag="VIO83_08630"
     CDS             1908..>4324
                     /locus_tag="VIO83_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY8331374.1"
                     /translation="MEIVTEFAYKVLEIEHCLIPLSDGTQLAARIWLPDTASVQAFPA
                     ILEYLPYRKRDGTALRDALTHPWMAGQGYVCVRVDMRGNGESQGLMADEYLLQEQDDA
                     LEVIDWLCQQPWCDGNLGMMGISWGGFNSLQVAARQPEALKAIITLCSTDDRYADDIH
                     YKGGNLLLENFGWAATMLNFSAAAPDPLLVGDAWKSLWQQRLDAMPLLAETWLQHQTR
                     DAYWRHGSVCEDYSAIKAAVYAVGGWGDAYKNAVPRLMQHLPGPKKAMIGPWIHKYPH
                     FAVPNPAIGFLQEARRWWDHWLKGIDNRVMDDAACTFYLQDVLAPKGSYAERPGIWVQ
                     TSGWPDPQVQWTDYSLNDHGLSPGQQALGGPRSICSPLTTGLHQGEYCAIWFGPDGPT
                     DQRRDDAQSLCFDSPPLAEPLALLGDVRLHLRLASDTPCGQLVARLNAVAPDGQVTQI
                     TYGVLNLALREDFSRLTPPVPGEPMDVQLNLDHVGVRVPAGHRLRLALSTASFPLLWP
                     SRELTTLTVLPAVQRVQLPIFSGAAVDAAYPLLGELLIAGQGARRAPAPGTVWVEPVL
                     LAQLGIEVGDRIELGYSAFTVAGVIADEPDRVAGAFSIGPRVLMNLDDLPATRLVSPQ
                     SRVRSRLLYDPPPTKAARLVSALEAELPPHTSVETADEGLDETRNMTRQAADFLRLVA
                     LAGVVLAAAAVVLSASNLTEKRLPAAATLKALGASRRLVLLTYAGLVAAVALLGGLSG
                     AAAGSVLERLLPILLSGLVPADLPPADVRMLPIAVLLALLVALAVAAPPLLRLGATPP
                     AAVLRAPA"
BASE COUNT          661 a         1425 c         1392 g          846 t
ORIGIN      
        1 tgggctgcgt agcggcccca aaaccatccc cccggtctac ctggaaaact cggcgcgacc
       61 ctactggggc cgctgcgcga cccaacgcgg gcaagcccgc tcgccacagc aatcgcggtt
      121 ttgctcttgc tcagccatga cctgacggaa tacccccata cccattggtc gattgcaccg
      181 cggccggcac ccacgcttat ctatagccac gcgaagactt caaggcggca cagtgcccgc
      241 ttctaacaat ggcctgcaaa ggcaataaaa atagtctgtc tggggaagcg cacgagatga
      301 aatacccatt gcgtcctttg gtcttctggc caacctttct gatcttgctg gcggcggtga
      361 tcgccagcta cgttgacctc accgcattcc tcgccgtgag caaacagctc aatacggtga
      421 tcctgcaaaa cttttcctgg ctattcagtg ccggttcttt cgccatgctg gtgctcacgg
      481 ccattgtgta tttttcgccc ctgggccagg tgcgtatcgg cggggagggc gccacgccgc
      541 tgttgagcaa atggcgctgg ttcatggtcg cgctgtgcac caccctggcg gtgggcgtgc
      601 tgttctggac cacggccgaa ccgctctacc atctgtactc gccaccctcc agcctgccga
      661 tcgaggcggg cagcgcggcg gccaagagct ttgccttgtc gacaatgttc ctgcactgga
      721 ccctcacgcc ctacgcgatt tacctggtgc cctccctggt gtttgccctg gtgttctaca
      781 acctgcgcag ccacttctcg attggcgcca tgttgcaacc cttgctcggc gccgagcggg
      841 tcaaacgcta tgccgggttg atcgacaccc tggcgatgtt cgccctggtc gcaggcatgg
      901 cgtcgtccct gggcaccggg gcgctgaccc tgtccggtgg cctgagccag tacatcggcg
      961 gtgagaccac gccgttgcgc ctgggcctga tcatcgcggt gatcgtgttc acctttgtcg
     1021 cctcggccgc cagcggcctg cagcgcggca tcgtcatgct gtcgtcgttc aacacctgga
     1081 tcatgctggc cctgggcgcg ttcgtgttgg tgtgtgggcc aaccctgtac atcttcagcc
     1141 tgggcgtcga gtccctgggg gtgtacctcg acaccttctt cagccgcagc ctgtccaccg
     1201 gtgccgccag tggcgacagc tggccgcaga gctggacggt gttctactgg tcggtgtggt
     1261 tcgcctgggc cccggtcagt gcgctgttcc tcggcaagat cggccggggc tacagggtgc
     1321 gcgaatttat ccagatcaat atgttgtacc cggcgctgtt taccgcgctg tggatctgca
     1381 ttttctccgg tagcagcctg tacttcgatg ccctcggcga tggcagtttg aaccgcgtgc
     1441 tcaacgagca aggcgtggag catgtgctgt accagatgtt ccagcagttg ccggccagcg
     1501 gcctgatgat cgcgtttttg ttgtttgttg cctttatctc ctttgtcacc gccgccgact
     1561 ccagcaccga cgtgatcgcc aacctgtgca gcaaaggcgt caccgccgat tcggacctgg
     1621 acggcaaccc gctgctgaag attgtctggg gcgtgatcat cggcacggtg tcgtgggtca
     1681 tggtggcctt tgtcggcatc gacggggtga agatgctctc caacctcggc ggcctgccgg
     1741 gaatgatcct ggtgctgctg gccagcacgt cgttgctcta ctggttgaag aaccccgcgc
     1801 tgctggacgt aaaggcccct gcggtccacg tcgaacctgg cctgcgtggt gccagccccg
     1861 tggcggattt tgcccgctga cttgcctctt tgttgctgga gttaccgatg gaaatcgtca
     1921 ccgaatttgc ctacaaggtc cttgaaatcg agcactgcct gatccccctg agcgacggca
     1981 cgcaactggc agcgcgtatc tggctgcctg acaccgccag tgtgcaggcc ttcccggcga
     2041 tcctcgaata cctgccctat cgcaagcgtg atggtacggc gctacgcgat gcgctgaccc
     2101 atccgtggat ggcggggcag ggctatgtct gcgtgcgggt cgatatgcgc ggtaatggcg
     2161 aatcccaggg cctgatggcc gatgagtacc tgttgcagga gcaggatgac gccctcgaag
     2221 tgatcgactg gctgtgccag cagccctggt gcgacggcaa cctgggaatg atgggcatct
     2281 cctggggcgg cttcaacagc ctgcaagtcg ccgcgcgcca gcccgaagca ttgaaggcga
     2341 tcatcaccct gtgctccacc gatgaccgct atgccgatga catccattac aagggcggca
     2401 acctgctgct ggagaacttc ggctgggccg cgaccatgct caacttcagc gcggcggcgc
     2461 ccgacccgct gctggtcggc gatgcctgga aaagcctgtg gcaacaacgc ctcgacgcca
     2521 tgccgctgct ggccgagacc tggctgcagc accagacccg cgatgcctat tggcgtcacg
     2581 gttcggtctg cgaggactac tcggcgatca aggcggcggt gtatgccgtg ggcggttggg
     2641 gcgatgccta caagaatgcg gtgccgcgcc tgatgcaaca tctgccggga ccgaagaaag
     2701 ccatgatcgg cccgtggatt cacaagtacc cgcacttcgc cgtgcccaac ccggccattg
     2761 gcttcctgca ggaggccagg cgctggtggg accactggct taaaggtatc gacaatcggg
     2821 tgatggacga cgctgcctgc accttttacc tacaggatgt gctggcaccc aagggcagtt
     2881 atgccgagcg cccagggatc tgggtgcaga cctcgggctg gcccgatccc caggtgcaat
     2941 ggaccgacta cagcctcaac gaccatggcc tcagccccgg ccagcaggca ctgggcggcc
     3001 cgcgcagtat ctgttcgcca ctgaccaccg gtctgcacca gggcgagtac tgcgccatct
     3061 ggttcggccc cgatggcccc accgatcagc gtcgcgacga tgcgcagtcg ctgtgtttcg
     3121 actcgccgcc gttggctgaa cccctggcgc tgctggggga tgtgcgcttg cacctgcgcc
     3181 tggccagcga taccccctgc gggcaactgg tggcacgtct gaatgcggtg gcgcccgacg
     3241 gccaagtcac gcagatcacc tacggcgtgc ttaaccttgc cctacgtgaa gatttctccc
     3301 gtctgacgcc gcctgtgccg ggcgagccca tggacgtgca attgaacctg gatcacgtcg
     3361 gcgtgcgcgt gccggccggc caccgcctgc gcctggccct gagtaccgcg agcttcccgt
     3421 tgctgtggcc cagtcgcgag ttgaccaccc tgactgtgct gcccgccgtg cagcgtgtgc
     3481 aattgccgat cttcagcggc gcggccgtgg acgccgccta cccgctgctg ggcgagctgc
     3541 tgatcgccgg tcagggggcg cggcgcgcgc cggcgcccgg cacggtgtgg gtggagccgg
     3601 tgttgctggc gcagctgggc atcgaggtgg gtgatcggat cgaactcggc tacagcgcgt
     3661 ttacggtggc gggcgtcatc gccgacgaac cggaccgcgt ggccggcgcc ttcagcatcg
     3721 gtccgcgggt gctgatgaac ctggacgatc tgccggctac gcgcctggtg tcgccgcaaa
     3781 gccgggtgcg cagccgactg ctctacgacc cgccgccgac gaaggctgct cgcctggtgt
     3841 cggcgctcga ggccgaactg ccgccccaca caagcgtcga aacggccgac gagggcctcg
     3901 acgagacacg caacatgacc cgccaggcag ccgattttct gcgcctggtg gccctggccg
     3961 gcgtcgtgct ggcggcggcg gcggtggtgc tgtcggcgtc caatctcacc gaaaaacgcc
     4021 tgccggcggc ggcgacactg aaagcgctcg gcgccagccg gcggctggtg ctgctgacct
     4081 atgccgggct ggtggccgcc gtcgcgttgc tgggtggctt gtccggtgca gcggccggca
     4141 gcgttctgga acggctgctg cccatcctgc tcagcggcct ggtgcccgcc gacctgccgc
     4201 cggccgatgt gcgcatgctg ccgatagccg tactgctggc actgctggtg gcgctggccg
     4261 tggccgcgcc gccgctgctg cgcctgggcg cgacgccgcc cgcggcggtg ctgcgcgcgc
     4321 ctgc
//
LOCUS       DATCUN010000224        14060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_179312, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000224 DATCUN010000000
VERSION     DATCUN010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 14060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14060
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179312"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VIO83_08635"
     CDS             complement(<1..1286)
                     /locus_tag="VIO83_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8331375.1"
                     /translation="MSGPDFGLLWQRAGFQVAESLLSVGPDDTYLRVVLSGVVNTLTV
                     AALALPLATVFGVVLGLLRLTRHPLLHRALALLVEPVRNTPVLLQLFVWYALLLQLPS
                     VRQAWQPLPGVFLSNRGLAMPLLHGAGGWMVILVATALVLWRTGRQPRWLQGAVLASG
                     FVALALAWHAVPPLQLDVPIAAGLGLQGGWSLSPEFTALVIGLTVFHAAYIADIVRGA
                     VLSVPAGQVDAASALGMRPATVAAQIVYPYAARVAVPPYANQCLMLVKNSTLAIAIGY
                     QELMAIVNTMIAQTGRAIEGMTLAAGFYVGISLLLAFFLARYNRRVNRHGIEGTGPLR
                     LRHRFGLPALRADALWASPVRAALSVIVLSVAALGLWLGLRWAVLDAVWAGPATACAD
                     AAGACWAAVTENSRLLLFGTLPAAQRHQASIACGLLL"
     gene            complement(1318..2364)
                     /locus_tag="VIO83_08640"
     CDS             complement(1318..2364)
                     /locus_tag="VIO83_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8331376.1"
                     /translation="MPSIPAFPSLRSVLLTAVAAAGTLCATLSHAGPTVDTIKKRGQL
                     VCGVSQGSAGLSLADSQGRWTGLDVDFCKALATAVLGSPDKVRYVPLSSQQRFPALLS
                     GEIDVLNRNTTVTSSRDAGLGITSTAIVFYDGQGFLVPKKLGITSAKNLDGAQVCVQP
                     GTNNEQNLVDWFTTHKLKYRPVVIENLVELEKAFYAGRCDVYLSDASTLAASRSARAG
                     NPDDYVILPERVNKSPLSPWVRDNDSNWIDIVRWTVNAVVTAEELGITSGNVDGFLVS
                     RDPQVRRLLGVDAGIGKSFQLDERWAYNVIKSVGNYGEIWDRNVGPKTPLKLERGYNA
                     LWNKGGLLYAPPFQ"
     gene            2397..3764
                     /locus_tag="VIO83_08645"
     CDS             2397..3764
                     /locus_tag="VIO83_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8331377.1"
                     /translation="MGLGVDRQGCRIEGTQILGMERALTSPREATDFAHAYAKNNQRR
                     LLEFAASLQGGDVDWQQRFRDYFPITAQKIYACQAYSSPLSPQVSQAVQAFFDGVTYA
                     RADKPEWLAAAESVRGQVGRLIGGAARSVAFTKNTTEGLNIVAHGFPWRDGDNVVIDD
                     QEHPVNVVPWLHLRTRGVEVRVAATEDRRVTVDALWQRVDARTRMVVVSYVQYSTGFR
                     ADIQTLAARCRNSGIRLVVDGIQAVGLLPIDVQGWGIDALACGAYKALLGPLGVGFLY
                     VAPDLLAELNPACLGASVANQIDRSVPDWTLRDRPPGDARHLEGGNINYPGVYGLARA
                     LIMIDEARVGRLAPWVLGLAAQLDAGLRGQGYQLLSSNREGENSFTVCLRHPEAFALR
                     KYLYAQGVVCNLLDTGALRLSMGGYNTPLEMDRVLEITERFASDATLRSQLMRPTAAQ
                     SYVRA"
     gene            complement(3749..5134)
                     /locus_tag="VIO83_08650"
     CDS             complement(3749..5134)
                     /locus_tag="VIO83_08650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010447296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8331378.1"
                     /translation="MVSSTRQFKLGAFLMQTGHHIAAWRHPGAQADAGVNFRHYVELA
                     QKAEAAKFDAIFLADSVGVRNSDLASLSRTARSDHFEPLTLLSAIAALTDRIGLIATV
                     STSFNEPFNVARKFASLDQISGGRSGWNLVTSSGTGEAQNFNRDEHFEHALRYERAAE
                     FHDVVKGLWDSWEDDSFVRDKESGQYFAEDKLHVLGHKGKHFSVRGPLNVARSPQGRP
                     VVVQAGASEAGRDLAARTAEVIFVAHQTFDEAKAFYADIKDRLPKHGRHPDDVKIMPG
                     IFPVVGRTQAEAEEKFEQLQALVHPVVGLSLLSNVIGGFDLSGLPVDGPLPALPETNG
                     PKSRQRLLLDLARRENLSIRELYLRIAGARGHQQVVGTPASIADQLQQWFEEGGADGF
                     NIMAPWFPGGLDDFITLVLPELRRRGLFRTEYEGRTLRDHLGLRRPVHPASAARLGRG
                     AQPVAERQART"
     gene            complement(5196..6062)
                     /locus_tag="VIO83_08655"
     CDS             complement(5196..6062)
                     /locus_tag="VIO83_08655"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008054973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8331379.1"
                     /translation="MSTLSTSSASNNELDIRGLGKRYTHAQAKSGELQVLEGIDLHVP
                     AGRFVSIVGASGCGKSTLLRLILGLDSQYEGQILLGGQAIAGTGRERGIVFQDHRLFP
                     WLTVAQNIAVGLRNAPFTAKEKTELVAEHVALVGLTGFERSWPHQISGGMAQRVAIAR
                     GLVNRPSVLLLDEPFGALDALTRSRLQNELQRIWQKEKITMLLVTHDVEEAVFLGDRV
                     VVMQPSPGRIRRIVDVDLPHPRNRSDPAFIALRDDVLSDFLDFDSDRAPLAAQPVGAA
                     DTGQPWRLQLAW"
     gene            complement(6070..6792)
                     /locus_tag="VIO83_08660"
     CDS             complement(6070..6792)
                     /locus_tag="VIO83_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019827375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8331380.1"
                     /translation="MVAVALWWLLSSLDVVNSALLVSPAKVFDTAVEQVVSGRLWRAL
                     SASLAREATGFTIGTVAGLLLGMLLGLSPMFNRIVGPSFNTFKQISLFAWIPLISVWF
                     GLGDVAKVVFLSLAALVPVVVNTCDGIRTVPRGLLEVARVYGFTRWQTVTQVVLPAAL
                     PAIFTGVYLALIYSWLATIGAEYLLVAGKGIGNTLIEGSEHFQMDLVIFGMVVIGTVG
                     WLMNAAARALERRLARWTGRTT"
     gene            complement(6882..7745)
                     /locus_tag="VIO83_08665"
     CDS             complement(6882..7745)
                     /locus_tag="VIO83_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008054975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8331381.1"
                     /translation="MSAEAELSAVLHGKGQHGPARASLPWRRFAATATRAADAALPWL
                     LPVALVALWFIGAEQGRISPQVLPPPLFVWETLRDLAVNGDLWLHVSTSFMRVGVGFA
                     VGTLLGLVLGSAMGLSRRFEAYVLPTFNALVQIPVLAWLPFVLLIVGIGEPLKYILIA
                     KAALVPVALNTLQGFRQTPVALREVGEVYGYSRRQQVLEIVLPHAVPTLFTGIRLGFT
                     KAWLSLVVVELVASSEGLGYLIVYGRQLFQLDLVMAAVIVVGAIGYAIDRLLDRLETA
                     VHRRQPGGAEP"
     gene            complement(7742..8827)
                     /locus_tag="VIO83_08670"
     CDS             complement(7742..8827)
                     /locus_tag="VIO83_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016783503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8331382.1"
                     /translation="MSSITRLVRWAATLTLALAASAHAAPDTIRIGVASAGGGDPVTF
                     GGSPGGVARSNNWLEDEFKASGIKVEWLFFKGAGPAVNEALSNKQIDFAYQGDLPQIV
                     GRSNGLKTKLLMVSGARNNLYLVTPPKSDIQSVKDLKDRNVSIFRGTNGHLVAINVLA
                     ASGLAERDVKGVNLDAGSAQAALVSNGVDAAFGGYEWFKVRDSGLAKVVYSTQGKDPA
                     LTRQAALLVRSEFEQANPAEVQRVVDVFVRAAHWSSEEKNRDELFRIWARSGTPVASW
                     AAEFDQQPLAIRNSPLVDDFIIARYKAVVADALKLKLIRREVSIDDWFDTRYLKASLK
                     KQGLEDYWTAFDAKGQPKKAGSSIASR"
     gene            complement(8824..10341)
                     /locus_tag="VIO83_08675"
     CDS             complement(8824..10341)
                     /locus_tag="VIO83_08675"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8331383.1"
                     /translation="MNDRLKRAHASRSAAVKAGLDHPVIDTDVHVNDFAPVLEDYVAQ
                     YGGNQLVDALRKALGGRFVTRNGGGKDWYQQSAEERQFHRTLRAPWWARVTRNSYDLA
                     TYTLPALLHERLGEQGSDYSILFPNDVLSPAAAGSEYRQPLHRAINHFHADQYRPYAD
                     RLTPVAGIPLHTPQEGIEELEFAVKTLGLKVINIAGGVRRPIRAIAEKYPAKEHPEIA
                     KQVGYIDFYGIDSEHDYDPFWAKVVELGVPVTTHYGSQGWTGRQSISNYMNNHIGHFA
                     DGSQAFAKALFFGGVTRRFPGLRVGLLEGGADWGSHVFTHLVDRFKKRNKHAVRNYDP
                     AEADIELLASLFEQYGGDLLKGRTLDKSTLLRDSLGISALPHSREPNESEIDDFALAG
                     IEKVEDIRDRWVNNFYFGSEADDRTVAAAFNDKVNPLGVKINAIWSSDVGHWDVPEFT
                     EPLAETWDLVQQGVISATDLKAFVFDNPHRFYTEANPRFFEGTEVGRKLALKDTK"
     gene            10599..12200
                     /locus_tag="VIO83_08680"
     CDS             10599..12200
                     /locus_tag="VIO83_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8331384.1"
                     /translation="MTTPWTPTRPMPQRAASAVLAVLAFAALTVTAQPRPGGTLTWLI
                     NPVPSSIVPLTTTAGGNAEIGPKVVEGLLTYDYELKPKPLLATAWSQSADGLQYTFTL
                     RKGVKWHDGKDFTSADVAFSILTLKQVHPRGRGTFANVVEVKTPDPHTAVIVLSKPSP
                     FLLTALASTESPIVPRHLYEGTDIASNKYNSAPVGTGPFMFKEWVQGSHIILERNPNY
                     WDKPKPYLERVVVRFVLDAAARAAALESGAADLANGTGGIPLSDVERFKKLPGIEIDA
                     RISPYLGSHQQIYFNLDTPVLQKREVRQAIAQAIDVNAFAKTVWYGYGTPSASPIGKG
                     IARYHDASIQPYPYNPKEAEAALDAAGLQRGAGGTRLKLRLLYNPFQDRRAADFVRQS
                     LGRIGIDAAVENYDFATYVTKAYTERAFDITLESLTNLFDPTVGVQRVFWSKNFKIGL
                     PFSNAPHYVNPEVDRLLEAAAVEVDEVKRRQLFVDFQQIVHRDIPSIELGANPQITVA
                     AKKVRNYAPTGENIRGSFADLYFQP"
     gene            complement(12259..13860)
                     /locus_tag="VIO83_08685"
     CDS             complement(12259..13860)
                     /locus_tag="VIO83_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8331385.1"
                     /translation="MFLSPPSFPLRSALLSALLAAGLAAHAAPPNHSGTLTLLTTSEP
                     TALVTIGNVATPILSVSAKVTEGLLKYDFDVNPQPQLATQWTISPDGLTYTFTLRRGV
                     KWHDGKDFTSADVAYSIELLKKIHPRGRNTFANLTAIDTPDKYTAVLRLSKPAPYLIR
                     ALVATETPIVPRHIYEGTDPGNNPNNNAPIGTGPFKFKEWVRGSHIVYERNTDYWDKP
                     KPFVDQLIVRFVTDPAAAAVAFETGTVDLGYRTPVPLADLERLKKVPALRFETKGNSY
                     SSNVTRLEFNLDNEYFRNEKVRQAVAHAVDRNVIVKVVNYGYGQVSYSPIAPGLKAFH
                     DPAPSPYAVDLKRANALLDEAGYPKKTGGVRFSVPLDFNPIGSDGARLADYLRSTLAR
                     IGIAVTVRAQDPSAFIKRIYTDRDFAFTTNGASNLFDPTVGVQRLYWSKNFIKGVPFS
                     NGTHYQNPVVDKLLEDAAVENDRAKRLKLFKDFQDTVARDVPDLNLYQPVFITIANQR
                     VHDHSLTADGVESNLADVWVDEAKK"
BASE COUNT         2360 a         4636 c         4796 g         2268 t
ORIGIN      
        1 aggagcagtc cgcaggcgat gctggcctgg tggcgctgcg cggccggaag ggtgccgaac
       61 aacagcagtc ggctgttctc ggtcaccgct gcccagcagg caccggccgc atcggcgcaa
      121 gcggtcgccg gccctgccca aaccgcgtcg agcacggccc atcggagccc gagccacagg
      181 ccgagcgccg cgaccgacag gacgatgacg ctgagtgccg cacgcaccgg cgacgcccag
      241 agcgcatcgg cccgaagcgc gggcaggccg aaccgatggc gcagccgcaa cgggcccgtg
      301 ccctcgatgc cgtggcgatt gacgcgccgg ttgtagcgtg ccaggaagaa ggccagcagc
      361 aggctgatgc cgacgtagaa gccggcggcc agcgtcatgc cttcgatcgc gcgcccagtc
      421 tgcgcgatca tcgtgttgac gatggccatc agttcttgat aaccgatggc aatggccagc
      481 gtgctgttct tgaccagcat caggcattgg ttggcgtaag gcggcaccgc gacgcgtgcc
      541 gcgtacgggt agacgatctg cgccgccacc gtggcgggcc gcatgcccag ggccgaggcg
      601 gcatcgacct gacccgcggg caccgacagc acggcaccgc gcacgatgtc ggcgatgtag
      661 gccgcatgga agaccgtcaa cccgatcacc agcgcggtga actccgggct caggctccag
      721 ccgccttgca ggcccaggcc cgctgcgatc ggcacatcga gctgcagcgg tggcacggcg
      781 tgccacgcca gcgccagtgc gacaaagccc gaggcgagca cagcgccctg cagccatcgg
      841 ggctgccggc cggtgcgcca gagcaccagg gccgtggcca cgaggatgac catccatccg
      901 cccgcgccat gcagcaaggg catcgcgagg ccgcggttgg acaggaagac gcccggcagc
      961 ggctgccacg cctggcgcac cgacggcagc tgcagcagca gcgcgtacca aacgaacaat
     1021 tgcagcagca ccggcgtgtt ccgcacaggc tcgaccagca gtgccaatgc acggtgcaac
     1081 agcggatggc gcgtgaggcg cagcagcccc agcaccacgc cgaacacggt ggcgagcggc
     1141 agggccaagg ccgcgaccgt cagcgtgttg accacaccgg acagcacgac acgcagatag
     1201 gtatcgtccg gcccgaccga caacaggctt tcggcgacct gaaagccggc ccgctgccag
     1261 agcaggccga aatcaggccc gctcatgccg cgaagctagc gcggcgtccc tcggtcttca
     1321 ctgaaacggc ggtgcgtaga gcaagccacc cttgttccac agggcgttgt agccgcgctc
     1381 gagcttgagc ggcgtcttcg ggccgacgtt gcggtcccag atctcgccgt agttgccgac
     1441 cgacttgatc acgttgtagg cccagcgttc gtcgagctgg aagctcttgc cgatgcccgc
     1501 gtcgacaccc agcaggcggc gaacctgcgg gtccctgctg accaggaagc cgtcgacgtt
     1561 gcccgacgtg atgcccagtt cttcggcggt caccacggcg ttgacggtcc aacgcacgat
     1621 gtcgatccag ttgctgtcgt tgtcgcgcac ccagggcgac agcggcgact tgttcacacg
     1681 ctcgggaagg atcacgtagt cgtcggggtt gccggcgcgt gccgagcggc tcgccgcgag
     1741 ggtcgatgcg tcggacaggt agacatcgca gcgtccggcg tagaaggcct tctcgagttc
     1801 gaccaggttc tcgatcacga ccggccggta cttgagcttg tgcgtggtga accagtcgac
     1861 caggttctgt tcgttgttgg tgccgggctg cacgcacacc tgcgcaccgt cgaggttctt
     1921 ggcgctggtg atgcccagct tcttcggcac caggaaaccc tgcccgtcgt agaagacgat
     1981 cgccgtcgac gtgatgccca ggcccgcgtc gcggctggag gtgacggtgg tgttgcggtt
     2041 cagcacgtcg atctcgccgg acaacagcgc cgggaagcgc tgctgcgaac tcaacggcac
     2101 gtagcgcacc ttgtccggag aacccagcac ggcggttgcc agggccttgc agaagtcgac
     2161 gtcgagcccc gtccaacgac cttggctgtc ggccagcgac aagccggccg acccctggct
     2221 gacgccgcag acgagctggc cgcgcttctt gatggtgtcg acggtcgggc cggcgtgcga
     2281 cagcgtggcg cacagcgtgc cggcagcggc aacagcggtc agcagaacgc tgcgcagcga
     2341 gggaaaagca ggaatcgacg gcatgaagag aggtccagga agaagcgagg gagaagatgg
     2401 gtctcggcgt cgatcggcag gggtgccgga tcgaagggac gcagattcta ggaatggaaa
     2461 gggctctgac ctcaccccgt gaagcgaccg atttcgcgca tgcttatgcg aaaaacaacc
     2521 aacgccgact cctagaattc gccgcttcac ttcaaggtgg cgacgtggat tggcagcagc
     2581 gcttcagaga ctatttcccg atcacggcgc agaagatcta cgcctgccag gcctacagca
     2641 gcccgctgtc gccgcaggtc tcgcaggcgg tgcaggcctt cttcgacggc gtgacctacg
     2701 cacgcgccga caagcccgaa tggctcgccg ccgccgagag cgttcgaggc caggtcggcc
     2761 gcctgatcgg cggtgccgcg cgcagcgtcg ccttcaccaa gaacacgacc gaaggcctga
     2821 acatcgtggc ccacgggttc ccctggcgcg acggcgacaa cgtggtgatc gacgaccagg
     2881 aacacccggt caacgtcgtg ccatggctgc atttgcgcac ccgcggcgtc gaagtgcgtg
     2941 tggccgccac cgaggaccgg cgcgtcacgg tcgatgcgct gtggcagcgg gtcgacgccc
     3001 gcacccggat ggtcgtggtg tcgtatgtgc agtacagcac cggcttccgg gccgacatcc
     3061 agaccttggc ggcgcgctgc cgcaacagcg gcatccggct ggtggtcgac ggcatccagg
     3121 ccgtcggctt gctgccgatc gacgtgcaag gctggggcat cgatgcgctg gcgtgcgggg
     3181 cgtacaaggc gctgctgggg ccgctgggcg tcggcttcct gtacgtggcc cccgacctgc
     3241 tggccgagct gaatccggcc tgtctgggcg catcggtcgc gaaccagatc gaccgcagtg
     3301 tgccggactg gaccctgcgc gacaggccac ccggcgacgc gcgccatctc gaaggcggca
     3361 acatcaacta ccccggcgtc tacggcctgg cgcgcgcctt gatcatgatc gacgaggccc
     3421 gggtggggcg gcttgcgccg tgggtactgg gcctggccgc gcagctcgat gccggcttgc
     3481 gcgggcaggg ctaccagctg ctctcatcca atcgcgaagg cgagaacagc ttcaccgtgt
     3541 gcctgcgcca ccccgaagcc ttcgcactgc gcaagtacct gtatgcgcaa ggcgtggtgt
     3601 gcaacctgct cgacacgggc gcgcttcgct tgtcgatggg cggctacaac acgccgctgg
     3661 agatggaccg cgtgctcgag atcacggaac gcttcgcctc cgatgcgacg ttgcgctcgc
     3721 aactgatgcg gcccactgcg gcccagtcct acgtgcgcgc ctgacgttcc gcgaccggtt
     3781 gcgccccgcg tcccaagcgc gccgcgctcg cgggatgcac cggtcgccgc agccccaggt
     3841 gatcgcgcag cgtgcggcct tcgtattcgg tgcggaacag gccgcgccgt cgcagttccg
     3901 gcagcacgag cgtgatgaag tcgtccaggc cgcccgggaa ccacggggcc atgatgttga
     3961 agccgtcggc gccgccttcc tcgaaccatt gctgcaactg gtcggcgatg ctggcgggcg
     4021 tgcccaccac ctgctggtgg ccgcgcgcgc cggcgatgcg caggtagagc tcgcggatgc
     4081 tgaggttctc gcggcgcgcc agatcgagca gcaggcgttg gcggctcttg ggcccgttcg
     4141 tctccggcag tgcgggcagc ggcccgtcga ccggcaggcc ggagaggtcg aagcccccga
     4201 tcacgttcga cagcagcgag aggccgacca ccggatgcac cagcgcctgc agctgttcga
     4261 acttctcctc ggcctccgcc tgcgtgcggc cgaccacggg gaagatgccg ggcatgatct
     4321 tcacgtcgtc ggggtggcgg ccatgtttcg gcagccgatc cttgatgtcg gcgtagaaag
     4381 ccttcgcctc gtcgaaggtc tggtgcgcga cgaagatcac ctcggccgtg cgcgcggcga
     4441 ggtcgcggcc ggcttcggac gcgccggcct gcaccaccac cggccgtccc tgcggtgacc
     4501 gcgcgacgtt gagcgggccg cgcaccgaga agtgcttgcc cttgtggccg agcacgtgca
     4561 gcttgtcttc ggcgaagtat tggccgctct ccttgtcgcg cacgaagctg tcgtcttccc
     4621 agctgtccca aagtcctttc accacgtcgt ggaactccgc cgcgcgctcg tagcgcagcg
     4681 catgctcgaa gtgctcgtcg cggttgaagt tctgcgcctc gccggtgccg ctggaggtca
     4741 ccaggttcca gcccgatcga ccgccgctga tctggtcgag cgaagcgaac ttgcgcgcca
     4801 cgttgaaggg ctcgttgaag ctcgtcgaca cggtcgcgat gaggccgatg cggtcggtca
     4861 gtgccgcgat ggccgacagc aatgtgagcg gctcgaaatg atcgctgcgc gcggtgcgcg
     4921 agagcgacgc cagatcgctg ttgcgcacgc cgacggaatc ggcaaggaag atggcatcga
     4981 acttggccgc ctctgccttc tgcgcgagct cgacgtagtg gcggaagttg acgccggcgt
     5041 cggcctgagc ccccggatgg cgccaggccg cgatgtggtg gccggtctgc atgagaaagg
     5101 cgccgagctt gaactggcgg gtggatgaaa ccatgggtcg agtgcctgaa gaggaaaagg
     5161 agaagaaaca aaagcctgcg aacggatcgc agcgatcacc acgccaactg cagccgccac
     5221 ggctggccgg tgtcggccgc accgaccggc tgtgcggcga ggggcgcgcg gtcgctgtcg
     5281 aagtcgagga agtcgctcaa cacgtcgtcg cgcagggcga tgaaggccgg atcgctgcgg
     5341 ttgcgcgggt ggggcaggtc gacgtcgacg atgcgccgga tgcgtcccgg cgatggctgc
     5401 atcaccacca cgcggtcgcc gaggaacacc gcctcttcca cgtcatgggt gaccagcagc
     5461 atcgtgatct tttccttctg ccagatgcgc tgcagttcgt tctgcagccg cgagcgggtg
     5521 agcgcgtcga gcgcaccgaa gggctcgtcg agcagcagca cgctggggcg gttgaccagg
     5581 ccgcgcgcaa tggccacgcg ctgggccatg ccgccggaga tctggtgggg ccacgagcgc
     5641 tcgaagccgg tcaacccgac cagcgccacg tgttccgcca ccagttcggt tttctccttc
     5701 gccgtgaaag gcgcattgcg caggccgacc gcgatgttct gcgcgaccgt gagccacgga
     5761 aagagccggt ggtcctggaa gacgatgcct cgctcgcgtc cggtgccggc gatggcctgg
     5821 ccgccgagca ggatctggcc ttcgtactgg ctgtcgaggc cgaggatgag gcgcagcagc
     5881 gtcgacttgc cgcagccgct ggcgcccacg atgctcacga agcggcccgc cggcacgtgc
     5941 aggtcgatgc cttcgagcac ctgcagctcg ccgcttttgg cctgcgcgtg ggtgtagcgc
     6001 ttgccgaggc cgcggatatc gagttcgttg ttggaggcag aggatgtcga aagagtgctc
     6061 attgggatct caggttgtgc ggccggtcca gcgcgccagc ctgcgttcca gcgcgcgggc
     6121 cgctgcattc atcagccagc cgaccgtgcc gatcacgacc atgccgaaga tcaccaggtc
     6181 catctggaaa tgctcgctgc cttcgatcag cgtgttgccg atgcccttgc cggccaccag
     6241 caggtactcg gcgccgatgg tcgcaagcca ggaatagatc agcgcgaggt acacgcccgt
     6301 gaagatcgcg ggcagggccg ccggcagcac cacctgcgtg accgtctgcc agcgcgtgaa
     6361 gccgtacacc cgcgccacct ccagcaagcc gcgcggcacg gtgcggatgc cgtcacaggt
     6421 gttcacgacc accggcacca gcgcggccag cgacaggaag acgaccttcg ccacgtcgcc
     6481 gaggccgaac cacaccgaga tcagcggaat ccatgcgaac agcgagatct gcttgaaggt
     6541 gttgaagctc ggtccgacga tgcggttgaa catcggcgac aggcccagca gcatgccgag
     6601 cagcaagcct gccacggtgc cgatggtgaa gccggtcgcc tcgcgcgcca ggctggcgct
     6661 caacgcgcgc cagagccgcc cgctcacgac ctgctcgacg gcggtgtcga acaccttggc
     6721 cggcgacacc agcaaggccg agttgacgac gtcgagagac gacagcagcc accacagcgc
     6781 cacggccacc atcggcaaca cgaaaccgcg ccagcggccg cgctgttcgg ggcgtagcgg
     6841 ctggaccacc gccagttcgg cggcgtctcg cgtggcgccg ctcatggctc ggccccgccg
     6901 ggttgtcgcc ggtgcaccgc ggtctcgagc cggtcgagca gccggtcgat ggcatagcca
     6961 atggcaccca ccacgatcac ggccgccatc accaggtcga gctggaacag ctgtcggccg
     7021 taaacgatga ggtagcccag cccttcgctg gaggccacca gctcgaccac caccagcgac
     7081 agccaggcct tggtgaagcc cagccggatg ccggtgaaga gcgtgggcac ggcatgcggc
     7141 agcacgatct ccaacacctg ctggcgccgg ctgtaaccgt agacctcgcc gacctcgcgc
     7201 agcgccacgg gcgtctggcg aaagccctgc aacgtgttga gcgccaccgg caccagggcc
     7261 gccttggcga tgaggatgta cttcagcggc tcgccgatgc ccacgatcag cagcacgaag
     7321 ggcaaccatg ccaggaccgg gatctgcacc agcgcattga aggtcggcag cacgtaggcc
     7381 tcgaaacgtc gcgacaggcc catggcggag cccagcacga gccccaacag cgtgccgacg
     7441 gcgaagccca cgcccacgcg catgaaactg gtgctcacgt gcagccacag gtcgccgttg
     7501 accgccaggt cgcgcagggt ttcccagacg aagaggggcg gcggcagcac ctgcggcgag
     7561 atccggcctt gctcggcgcc gatgaaccac aacgccacca gcgcaacggg cagcagccag
     7621 ggcagggcgg cgtccgccgc tctcgtggcc gttgcggcaa agcgtcgcca ggggagcgat
     7681 gcacgcgccg gcccgtgctg gcccttgccg tgcaataccg cggacagctc ggcttcggcg
     7741 ctcatcgcga ggcgatcgac gagcccgctt tcttgggctg gcccttggca tcgaaggccg
     7801 tccaatagtc ctccagaccc tgcttcttca gcgatgcctt caggtagcgc gtgtcgaacc
     7861 agtcgtcgat cgacacctcg cggcggatca gcttgagctt gagcgcgtcg gccaccacag
     7921 ccttgtagcg cgcgatgatg aaatcgtcga ccaacggcga gtttctgatg gccaggggct
     7981 gctggtcgaa ctcggccgcc catgacgcga cgggcgtgcc gctgcgcgcc cagatgcgga
     8041 agagctcgtc gcggttcttc tcttccgacg accagtgcgc ggcgcgcacg aacacgtcga
     8101 ccacgcgctg cacctcggcc gggttggcct gctcgaattc gctgcgcacg agcagcgcgg
     8161 cctggcgcgt caaggccggg tccttgccct gcgtggaata caccaccttg gccagccccg
     8221 agtcgcgtac cttgaaccat tcgtaaccgc cgaaggccgc gtccacgccg ttggacacca
     8281 gcgcggcttg cgcgctgccc gcgtcgaggt tcacgccctt gacgtcgcgc tcagccaacc
     8341 cgcttgctgc cagcacgttg atggcgacca gatgcccgtt ggtgccgcga aagatcgaca
     8401 cgttgcggtc cttcaggtcc ttcaccgact ggatgtccga cttgggtggc gtcaccaggt
     8461 aaaggttgtt gcgggcgccg ctcaccatca gcagcttggt cttcagcccg ttggagcgcc
     8521 cgacgatctg cggcaggtcg ccctggtagg cgaagtcgat ctgcttgttc gacagcgcct
     8581 cgttcaccgc cggccccgcg cccttgaaga acagccactc gaccttgatg cccgaagcct
     8641 tgaattcgtc ctccagccag ttgttgctgc gcgccacgcc gccgggcgag ccaccgaagg
     8701 tgaccgggtc gccaccgccg gccgatgcga cgcctattcg gatggtgtcg ggcgccgcat
     8761 gggccgatgc ggccaaggcc agggtgagcg tcgcggccca gcgcacaaga cgggtgatgc
     8821 tgctcatttc gtgtccttca gcgcgagctt gcggccgact tcggtgcctt cgaagaagcg
     8881 tggattcgct tcggtgtaga agcggtgcgg gttgtcgaag acgaaggcct tcaagtcggt
     8941 ggcggagatc acgccctgct gcaccaggtc ccaggtctcg gccagcggct cggtgaactc
     9001 gggcacgtcc cagtggccca cgtcggacga ccagatcgcg ttgatcttca cgcccagcgg
     9061 attgaccttg tcgttgaagg cggcggccac cgtgcggtcg tcggcttcgg agccgaagta
     9121 gaagttgttc acccagcggt cgcggatgtc ttcgaccttc tcgatgccgg ccagcgcgaa
     9181 gtcatcgatc tccgattcgt tcggctcgcg gctgtgcggc aacgcggaaa tgcccaggct
     9241 gtcgcgcagc agcgtggact tgtcgagcgt gcggcccttc agaaggtcgc ccccgtattg
     9301 ctcgaacagc gaggcgagca gttcgatgtc ggcctccgcc gggtcgtagt tgcgcaccgc
     9361 gtgcttgttg cgctttttaa agcggtctac caggtgtgtg aagacatgcg aaccccagtc
     9421 ggcgccgcct tcgagcaggc ccacacgcag cccgggaaag cgccgggtca cgccgccgaa
     9481 gaacagcgcc ttggcgaagg cttgcgagcc gtcggcgaag tggccgatgt ggttgttcat
     9541 gtagttgctg atcgactggc gcccggtcca gccctggctg ccgtagtgcg tggtaaccgg
     9601 cacgccgagt tcgaccacct tggcccagaa cgggtcgtag tcgtgctcgc tgtcgatgcc
     9661 gtagaagtcg atgtagccga cctgcttggc gatctcgggg tgttccttgg ccggatactt
     9721 ctcggcgatg gcgcggatcg gccggcgcac gccgccggcg atgttgatca ctttcaggcc
     9781 cagcgtcttg actgcgaact cgagctcctc gatgccctcc tgcggcgtgt gcagcgggat
     9841 gcccgcgacc ggggtcaggc ggtcggcata cggccggtac tggtcggcat ggaagtggtt
     9901 gatcgcgcgg tgcaacggct ggcggtactc gctgccggcc gcggccggcg agagcacatc
     9961 gtttggaaac aggatcgaat agtccgagcc ttgctcgccc aggcgctcgt gcagcagcgc
    10021 gggcagcgtg taggtggcca ggtcgtagct gttgcgcgtc acgcgggccc accagggcgc
    10081 ccgcagcgtg cgatggaact ggcgttcctc ggcactttgt tggtaccagt ccttgccgcc
    10141 accgttgcgc gtgacgaatc gcccacccag cgccttgcgc aacgcatcga ccagctggtt
    10201 gccgccgtac tgcgccacgt agtcctccag caccggcgcg aagtcgttga catggacatc
    10261 cgtgtcgatc accggatggt cgaggccggc cttgacggcg gcggagcgcg aagcgtgcgc
    10321 gcgtttcaaa cgatcgttca tctgcgggtt cctcgatggg ggaaggatcc gtgcaggcta
    10381 cgcgccggcc gtgcgctcgc caaagaaggc ttttggtgtt gcttagccgt tgcttgcata
    10441 aggcgaggcc gcgcgaggag gagcgaagag ggcatgccgc gcatgcagga aaaacagata
    10501 tcgaagcgcg catccgttgt ttggcccgcc gcaacgcgcg gctaacctgc ggccccgctg
    10561 catgtgatgc ggcctcttcg gtttttcgag aaaaagacat gacgacaccc tggactccca
    10621 cgcggccgat gccgcagcgc gcggcatcgg ccgtgctcgc cgttctcgcc ttcgcagcac
    10681 tgaccgtcac ggcccagccg cggccgggcg gcacgctgac ctggctgatc aaccctgtgc
    10741 cctcgtcgat cgttccgctc accaccacgg caggcggcaa tgccgagatc ggcccaaagg
    10801 tggtcgaggg cctgttgacc tacgactacg agctcaagcc caagccgctg ctcgccaccg
    10861 cctggtcgca gagcgcggac ggcctgcagt acaccttcac gctgcgcaaa ggcgtgaagt
    10921 ggcacgacgg gaaggacttc acctccgccg atgtcgcgtt ctcgatcctc acgctcaagc
    10981 aggtgcatcc gcgcggccgc ggtaccttcg ccaacgtggt ggaggtgaag acgcccgacc
    11041 cgcacacggc cgtcatcgtg ctgagcaagc cctcgccgtt cctgctgacg gcgctggcct
    11101 ccaccgagtc gccgatcgtg ccgaggcacc tctacgaggg caccgacatc gcgtccaaca
    11161 agtacaacag cgcaccggtg ggcaccgggc ctttcatgtt caaggagtgg gtgcagggca
    11221 gccacatcat cctggagcgc aatccgaact actgggacaa gcccaagccc tacctcgaac
    11281 gcgtggtggt gcgcttcgtg ctcgacgccg cggcgcgtgc agccgcgctg gagtcgggcg
    11341 cggccgactt ggccaacggc accggtggca ttccgctgtc ggacgtggag cggttcaaga
    11401 agctccccgg catcgagatc gacgcccgca tctcgcccta cctcggcagc caccagcaga
    11461 tctacttcaa cctggacacg ccggtgctgc agaagcgcga ggtgcgacaa gcgattgcgc
    11521 aggccatcga cgtcaacgcg ttcgccaaga cggtgtggta cggctacggc acgccgtcgg
    11581 cttcgccgat cggcaagggc attgctcgct accacgacgc gagcatccag ccttatccgt
    11641 acaacccgaa ggaggccgag gcggcgctcg atgcggccgg cctccagcgc ggcgcaggcg
    11701 gcacgcgcct gaagctgcgg ctgctctaca accccttcca ggatcggcga gcggcggact
    11761 tcgtgcgcca gtcgctgggc cgcatcggca tcgacgcggc ggttgagaac tacgacttcg
    11821 cgacctacgt caccaaggcc tacaccgaac gggcgttcga catcacgctg gagagcctca
    11881 cgaacctgtt cgatcccacg gtcggtgtgc agcgcgtgtt ctggtcaaag aacttcaaga
    11941 tcggcctgcc gttctccaac gcgccgcact acgtgaaccc ggaggtggat cgcctgctgg
    12001 aggccgcggc ggtcgaggtg gacgaggtca agcgccgcca gctcttcgtc gacttccagc
    12061 agatcgtgca ccgtgacatc ccgtcgatcg agctgggcgc caatccgcag atcacggtgg
    12121 cggcgaagaa ggtgcgcaac tatgcgccga ccggcgagaa catccgcggg agcttcgcgg
    12181 acctgtactt ccagccgtga accgagtcgt ggtttcgttg gaccagaggc ccgggtcgca
    12241 agcctcgggc tcctcgggct acttcttcgc ttcgtccacc cacacgtccg ccagatttga
    12301 ttccaccccg tccgccgtca gcgagtggtc gtgcacgcgc tggttggcga tggtgatgaa
    12361 caccggctgg tagaggttca ggtcgggcac gtcgcgtgcc acggtgtcct ggaagtcctt
    12421 gaagagcttc agccgcttgg cgcggtcgtt ctccacggcc gcgtcttcca gcagcttgtc
    12481 caccaccggg ttctggtagt gggtgccgtt ggagaagggc acacccttga tgaagttctt
    12541 cgaccagtac agccgctgca cgcccaccgt tgggtcgaac aggttgctcg cgccgttggt
    12601 ggtgaaggcg aagtcgcggt cggtgtagat gcgcttgatg aaggcgctcg ggtcctgcgc
    12661 acgcacggtc accgcgatgc cgatgcgcgc cagtgtcgag cgcaggtagt cggccaggcg
    12721 cgcgccgtcg gaaccgatcg ggttgaagtc cagcggcacg ctgaagcgca cgccaccggt
    12781 cttcttcgga tagccggctt cgtcgagcag cgcattggct ctcttcaggt cgacggcgta
    12841 gggagagggc gccgggtcgt ggaaggcctt caggccgggc gcgatcggcg agtagctgac
    12901 ctggccgtag ccgtagttca ccaccttgac gatcacgttg cggtccaccg cgtgggccac
    12961 ggcctggcgc accttctcgt tcctgaagta ctcgttgtcg aggttgaact ccagccgcgt
    13021 cacgttcgac gaataactgt tgcccttggt ctcgaagcgc agcgcgggca ccttcttcag
    13081 gcgctccagg tcggccagcg gcacgggcgt gcggtagccc aggtcgacgg tgccggtctc
    13141 gaaggccacg gcggcggccg ccgggtcggt cacgaagcgc acgatcaact ggtcgacgaa
    13201 gggcttgggc ttgtcccagt agtccgtgtt gcgttcgtac acgatgtggc tgccgcgcac
    13261 ccattccttg aacttgaacg ggccggtgcc gatgggcgcg ttgttgttcg ggttgttgcc
    13321 ggggtcggtg ccttcgtaga tgtgccgggg cacgatgggc gtctcggtcg cgaccagcgc
    13381 gcggatcagg tacggcgcgg gcttggacag ccgcagcacg gccgtgtact tgtcgggcgt
    13441 gtctatggcc gtgaggttgg caaaagtgtt gcgcccgcgc gggtggatct tcttgagcag
    13501 ctcgatggaa taggccacgt cggcggaggt gaagtccttg ccgtcgtgcc acttcacgcc
    13561 ccggcgcagc gtgaaggtgt aggtcaggcc gtcgggactg atcgtccact gggtggcaag
    13621 ctgtggctgc gggttgacgt cgaagtcgta cttgagcagg ccttcggtga ccttggcgct
    13681 cacgctcagg atgggcgtag cgacattgcc gatggtgacg agcgcggtcg gctcgctggt
    13741 cgtcagcagc gtgagcgtgc cgctgtggtt gggcggtgcg gcgtgggcgg cgaggcccgc
    13801 ggcaagcagc gcggagagga gggcgctgcg aagcgggaag gaggggggcg agagaaacat
    13861 ggttaaaaca gggtcggtag gaaaagaaga agtcaggcga tgacaggccc gacgggtgcc
    13921 aagccggctt gtgcagcgga ggccgaagaa gcctccggcg acaccagccc ttcgcgcagg
    13981 tcgcgcgcaa ccagcgccgg gtcgcgctcg gacgggtcgc ccaggccacc gccaccgggc
    14041 gtctcgacga tcaggcgatc
//
LOCUS       DATCUN010000225         3649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_179334, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000225 DATCUN010000000
VERSION     DATCUN010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179334"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..>3649
                     /locus_tag="VIO83_08690"
     CDS             266..>3649
                     /locus_tag="VIO83_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HEY8331386.1"
                     /translation="MEKVAAATNARGARSTTKQKAVSLDAALADDAWGWDSMASSCCS
                     GNRARFISLRKCDVVPVKVADGGVVHASHIGSVALRVVTDSGKVVRIVVDNVLYHERF
                     ASNLLSSELLTKKLGWQHHSTPEASYVVTPGGHRVTLSTRGRVAVLMSAGPERVMLAQ
                     AGAAGAVSDADADALVRLHEKMAHRGWTRMMHMLNSGRVLDHGIDLKSMSSSSIKAAE
                     ARVRECTACVKGKATRTPFGHRGLDRGTKAGECLHMDTYQVKVERDGRMVVEYGLVVK
                     DMFSGYMWHAQLRSKDEVADYVAVLVRQSETQFGCTVKRLYADGGTEFINQTLKGFCS
                     KHGKELRWTPARTQQLNGAAERSVRTFKDYERTMVQHAGAPLKLWGRAGWHAAYVWNR
                     THISKDTGMTPYEAMRGKKPSLRHINVWGCDAYCHVPKEQRSGLEPKAEPCIYLGHND
                     AQNAAYVLPLSTRKVITSRDVTYRSGSFTFMHALARGDDGVRDALAQVPEAELRSEAD
                     GEPDRVPAQGGQDGQPAAASSDSESDDTSESDPEEEYVVESIVGQRRRNGRLEFKVHW
                     AGYGAEEDSWEPESAVSDLAALDAWQAGQRRSKRLESEHKVDCDEADDEPQVHMAMSA
                     LRQLQLPEERPEPAAVMSAVATGIAALEAQTPQTYRQAMASPDAAKWKAALDKEMRSC
                     TEQQVWTLVRRDQLPPRANVLPCKEVFKLKLDEHGAVVEHKARFTPKGFRQKQGVDFF
                     ETFAHTGQYKTFRGSLSLVAKWNHELAQFDVPTAFLNAPVDEDIYMQLPEGYEQPGMV
                     CKLQKSLYGLKQAPRNWDKLVHTFITKDMAFKATVSDRSLYFKRSRSGRLMMIYRFVD
                     DMQGSYHADDAAEFQQSVALLQQRFNIKQLKTTTWMLGMRITRDRKARTITLDQELYV
                     SKALEKFGLQQCRVVSTPEAVGAAHDANPALDKPADRQRYMEMTGTLMYAAISTRPDI
                     AHAVHYLASNMQAPTQRHMQAAERVFRYLAGTKALGLVFGSRNGDTVGDSRGRRAQVQ
                     VDVCAFSDADWANDKGDRRSITGWVAKLNGDPVSWSSKKQRVVALSTCEAELYAESAA
                     IQEVLWLRGLMEELGLHTQTGSTVYGDNQSAIAVSENGVK"
BASE COUNT          707 a         1125 c         1320 g          497 t
ORIGIN      
        1 cgtgaagcgc aaggaggcca accagcagag tgctcctctg ctggaagggc cgggatgtgc
       61 cagtgggggc gcgcccctcg ctgatgctac cctggtacga gagaagagcc gagctgcagc
      121 cgccgcgccg acccctccgg cagagcccct caagagagcg gctgtcggcg cggtgggatc
      181 ctgctcggct cctccctcga ctctggcggt gtcggcggcc aagtcggctg ctgcaccgcc
      241 tgcgctcacg caacagggtc ccgcagtgga gaaggtggct gcggccacca atgcccgggg
      301 cgcccgctcg acgaccaagc agaaggcagt gtcgctggac gcagcactgg ctgatgatgc
      361 ctggggctgg gacagcatgg cgtcgtcctg ctgcagcggc aaccgcgctc gattcatctc
      421 gctgcgcaag tgcgatgtgg tgcccgtcaa ggtggcggac ggtggcgtcg tccatgccag
      481 ccacattggg agcgtggcgc tgcgtgtggt gacggacagc ggcaaggtgg tgcgcatcgt
      541 cgtcgacaac gtgctgtacc acgagagatt cgcgtcgaac ctgctgagca gcgagcttct
      601 gaccaagaag ctgggctggc agcaccacag caccccggag gcgagctacg tcgtcacgcc
      661 cggagggcac cgcgtgacgc tcagcacgcg cggcagagtg gcggtgttga tgagcgccgg
      721 accggagcgc gtgatgctcg cccaagcggg ggccgctggg gcggtcagcg acgcagacgc
      781 ggacgcgctg gtgcgcctgc acgagaagat ggctcatcgc ggctggacac gcatgatgca
      841 catgctcaac agcggccgag tgctcgacca cggcatcgac ctgaagagca tgagcagcag
      901 cagcatcaag gcggcagagg cgcgcgttcg tgagtgcacg gcgtgcgtca agggcaaggc
      961 gacgcggact ccctttgggc atcgcggcct ggaccgcggc accaaggcag gcgagtgctt
     1021 gcacatggac acgtaccagg tgaaggtgga gcgcgatggc cgcatggtcg tggagtacgg
     1081 cctggtggtg aaggacatgt tctccggcta catgtggcac gcccagctgc ggagcaagga
     1141 cgaggtcgcg gactacgtcg cagtccttgt tcgccagtcg gagacgcagt tcggctgcac
     1201 ggtcaagcgc ctctacgccg acggcggcac ggagttcatc aaccagacgc tgaaggggtt
     1261 ctgctccaag cacgggaagg agctgcgctg gacgccggcg cgcacccagc agctcaatgg
     1321 ggccgccgaa cgctcggtgc gcaccttcaa ggactacgag cgcaccatgg tgcagcacgc
     1381 gggcgcgccg ctgaagctct ggggccgggc gggatggcac gctgcgtacg tgtggaaccg
     1441 cacgcacatc tcgaaggaca ccgggatgac gccgtacgag gcgatgcgcg gcaagaagcc
     1501 gagcctgcgc cacatcaatg tgtggggctg cgacgcctac tgccacgtgc cgaaggagca
     1561 gcgcagcggg ctggagccca aggcggagcc gtgcatctat ctgggccaca acgacgcgca
     1621 gaacgcggcg tacgtgctcc cgctcagcac gcggaaggtg atcacctcgc gcgacgtgac
     1681 gtaccgcagc ggcagcttca ccttcatgca cgcgcttgcg cgcggcgacg acggcgtgcg
     1741 cgacgcgctg gcacaggtgc cggaggctga gctgcgctcc gaggccgacg gagagccaga
     1801 tcgagtgccg gctcaggggg ggcaggatgg ccagcccgcc gcggcgagca gcgactccga
     1861 gagtgacgac acctccgagt cggaccctga ggaggagtac gtcgtggaga gcatcgtggg
     1921 tcagcgcaga cgcaacggcc gcctcgagtt caaggtgcac tgggctggct acggcgcgga
     1981 ggaggacagc tgggagcccg agtcggctgt cagcgacctc gcagccctgg acgcctggca
     2041 ggccgggcag cgacgctcca aacgcctgga gtccgaacac aaggtcgact gcgacgaggc
     2101 cgatgacgag ccgcaggtgc acatggcgat gagcgcgctg cgccagctgc agctgccgga
     2161 agagcgccca gagcccgccg cggtgatgag cgctgtggcc acgggcattg ccgcattgga
     2221 agcgcagacg ccacagacct accgccaggc catggccagc ccggacgcgg ccaagtggaa
     2281 ggcggcgctg gataaggaga tgcgctcctg caccgaacag caggtgtgga cgctcgtgcg
     2341 acgagaccag ctgccgccca gggccaacgt gctgccgtgc aaggaggtct tcaagctgaa
     2401 gctggacgag cacggcgccg tggtggagca caaggcgcgc ttcacgccga aaggcttccg
     2461 gcagaagcag ggagtggact tcttcgagac cttcgcgcac accggccagt acaaaacctt
     2521 tcgtggctcg ctctccctgg tggccaagtg gaaccacgag ctggcgcagt tcgacgtgcc
     2581 gacggcgttc ctgaatgcgc ctgtcgacga ggacatctac atgcagctgc cggaagggta
     2641 cgagcagccc ggcatggtct gcaagctgca gaagtcgctg tacggcctga agcaggcgcc
     2701 gcgcaactgg gacaagctgg tgcacacatt catcacgaag gacatggcat tcaaggcgac
     2761 agtgagcgac cggtcgctgt acttcaagcg cagccgcagc ggaaggctga tgatgattta
     2821 ccgcttcgtc gacgacatgc agggcagcta tcacgcagac gacgcggcgg agttccagca
     2881 gagcgtggcg cttctgcagc aacgtttcaa catcaaacaa ctgaagacga cgacgtggat
     2941 gctgggcatg cgcatcacgc gcgaccgaaa ggcgcgcacg atcacattgg atcaggagct
     3001 gtacgtcagc aaggcgctgg agaagttcgg cctgcagcag tgccgcgtgg tcagcacgcc
     3061 cgaggcagtg ggtgcggcgc acgatgcgaa tccggcgctg gacaagcctg cagacagaca
     3121 gcggtacatg gagatgacag gcacgctcat gtacgcggcg atcagcacgc ggcccgacat
     3181 cgcgcatgca gtgcactatc tggcgagcaa catgcaggcg ccgacccagc gccacatgca
     3241 ggcggccgag cgcgtgttcc ggtacctggc ggggacgaag gcgctcggac tggtgttcgg
     3301 ctcgcgcaac ggcgacacgg tgggcgactc gcgggggcgc cgggcgcagg tgcaggtcga
     3361 cgtgtgcgcc ttctccgacg ccgactgggc caacgacaag ggcgacagga ggtcgatcac
     3421 cggctgggtg gcgaagctca acggcgaccc ggtgagctgg tcgagcaaga agcagcgcgt
     3481 ggtggcgctg tcgacgtgcg aggcggaact gtacgccgag tcggctgcga tccaggaggt
     3541 gctgtggctg cgcggcctga tggaggagct gggcctgcac acacagacag gatccacagt
     3601 gtacggcgac aaccagtcag ccatcgccgt cagcgagaac ggcgtcaag
//
LOCUS       DATCUN010000226         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_197538, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000226 DATCUN010000000
VERSION     DATCUN010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197538"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1097
                     /gene="glyQ"
                     /locus_tag="VIO83_08695"
     CDS             102..1097
                     /gene="glyQ"
                     /locus_tag="VIO83_08695"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_064729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY8331387.1"
                     /translation="MSQTTPAVRTFQDLILALQSYWAEQGCVVLQPYDMEMGAGTFHT
                     ATFLRALGPETWNAAYVQPSRRPADGRYGENPNRLQHYYQFQVVLKPNPDNIQDLYLG
                     SLRAIGIDPLVHDIRFVEDNWESPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGVECYP
                     VTGEITYGLERLAMYLQGVDSVYDLVYSDGPFGKVTYGDVFHQNEVEQSTYNFEHANV
                     DKLFELFDFYEAEANRLMALELPLPTYEMVVKASHTFNLLDARRAISVTARQQYILRV
                     RTLARAVAQSYLIARAKLGFPMATAELRDEVLAKLSESGLISDEVLGNKVLKEAE"
     gene            1097..>2052
                     /gene="glyS"
                     /locus_tag="VIO83_08700"
     CDS             1097..>2052
                     /gene="glyS"
                     /locus_tag="VIO83_08700"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_064728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY8331388.1"
                     /translation="MSAQDFLVELGTEELPPKALKTLAEAFLAGVEKGLKAAGLGYAS
                     ARYYAAPRRLAVLVEALATQQPDRSVNLDGPPVQAAFDKDGQPTQAALGFARKCGVDL
                     EQIDRSGAKLKFVQSIPGQPAASLLPGIVETSLNELPIPKRMRWAARREEFVRPTQWL
                     VMLFGDSVIDCTILTQQAGRVSRGHRFHANHDVRISAPANYAEDLRSAYVIADFAERR
                     TLIDARVAELASEQHGQAIVPPALLDEVTALVEWPVPLVCSFEERFLEVPQEALITTM
                     QDNQKYFCLLDANGKLLPRFITVANIESKDPAQIVSGNEKVVR"
BASE COUNT          362 a          749 c          626 g          315 t
ORIGIN      
        1 ggcgaccggg tgcacacgcc gagcgcgcgc ccccactgcg gcgcgcaccg cgcttcggtt
       61 atactcggcg gctttacgca agcctcagaa caggtgattt cgtgagccag actacgcctg
      121 ccgtgcgcac cttccaggac ctgatcctcg ccctgcaaag ctactgggcg gagcagggct
      181 gcgtggtatt gcagccctac gacatggaaa tgggcgccgg caccttccac accgccacct
      241 tcctgcgcgc cctcggtccc gagacctgga acgccgccta cgtgcagccc tcgcgccgcc
      301 cggccgacgg ccgctacggc gagaacccca accgcctgca gcactactac cagttccagg
      361 tggtgctcaa gccgaacccg gacaacatcc aggatctgta tctcggctcg ctgcgcgcca
      421 tcggcatcga cccgctggtc cacgacatcc gcttcgtcga ggacaactgg gaatcgccga
      481 ccctcggcgc ctggggtctg ggctgggaag tctggctcaa cggcatggaa gtcacccagt
      541 tcacctactt ccagcaggtc ggcggcgtcg agtgctaccc agtgaccggc gagatcacct
      601 acggcctcga gcgcctggcc atgtacctgc agggcgtcga ctcggtgtac gacctggtct
      661 actccgacgg cccgttcggc aaggtcacct acggcgacgt gttccaccaa aacgaggtgg
      721 agcagtccac ctacaacttc gagcacgcca acgtcgacaa gctgttcgag ctgttcgact
      781 tctacgaggc cgaagccaac cgcctgatgg ccctcgagct gccgctgccg acctacgaga
      841 tggtggtcaa ggcctcgcat accttcaacc tgctcgacgc ccgccgcgcc atctcggtga
      901 ccgcgcgcca gcagtacatc ctgcgcgtgc gcaccctggc ccgcgccgtg gcgcagagct
      961 acctgatcgc ccgcgccaag ctcggcttcc cgatggccac cgccgaactg cgtgacgaag
     1021 tcctggccaa gctgtccgag tcaggactga tttcggacga agtcctgggc aacaaagttc
     1081 tgaaggaggc cgaatgatga gcgctcaaga tttcctggtc gagctgggca ccgaagagct
     1141 gccacccaag gcgctgaaga ccctcgccga ggccttcctc gccggcgtcg agaagggtct
     1201 caaggccgcc ggcctgggct acgccagcgc gcgctactac gccgcgccgc gccgcctggc
     1261 ggtgctggtc gaggcgctgg ccacccagca gccggaccgc agcgtcaacc tcgacggccc
     1321 gccagtacag gccgccttcg acaaggacgg ccagccgacc caagctgcgc tgggcttcgc
     1381 ccgcaagtgc ggcgtcgatc tcgagcagat cgaccgcagc ggcgcgaaac tcaagttcgt
     1441 gcagagcatc cctggccagc cggccgccag cctgctgccg ggcatcgtcg aaacctcgct
     1501 gaacgagctg ccgatcccca agcgcatgcg ctgggccgcc cgccgcgagg agttcgtgcg
     1561 tccgacccag tggctggtga tgctgttcgg tgacagcgtg atcgactgca ccatcctcac
     1621 ccagcaggcc ggacgtgtgt cccgcggcca tcgcttccac gccaaccacg acgtgcgcat
     1681 ctccgctccg gccaactacg ccgaggacct gcgcagcgcc tacgtgatcg ccgacttcgc
     1741 cgagcgccgc acgctgatcg acgcccgcgt cgccgagctg gcaagcgagc agcacggcca
     1801 ggccatcgtg ccgccggccc tgctggatga agtgaccgcg ctggtcgagt ggccggtgcc
     1861 gctggtctgc tccttcgagg aacgcttcct cgaggtgccg caggaagcgc tgatcaccac
     1921 catgcaggac aaccagaagt acttctgcct gctcgacgcc aacggcaagc tgctgccgcg
     1981 cttcatcacc gtggccaaca tcgagtccaa ggacccggcg cagatcgtct ccggcaacga
     2041 gaaggtggtg cg
//
LOCUS       DATCUN010000227         7720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_122345, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000227 DATCUN010000000
VERSION     DATCUN010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122345"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VIO83_08705"
     CDS             <1..503
                     /locus_tag="VIO83_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009047871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8331389.1"
                     /translation="WLAVEDPAAPWPALGKAGKSAGPFYLVWTNPEAEQIGPEQWPFR
                     MVRLRVLAPVAERFPALRPDPALAASDPVNLGFAQFQKHCLACHRLNKQGDATFGPDL
                     NLPYNPTEYLRADLLRRYIRDPQALRHWPQARMPGFSEAALSEAQLDQLLAYLRHMAG
                     RKVAAD"
     gene            588..974
                     /locus_tag="VIO83_08710"
     CDS             588..974
                     /locus_tag="VIO83_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8331390.1"
                     /translation="MRRLLLALSLILPLSACQADTPPQTLAAIQNGSHVLIDVRTGEE
                     FAAGHLQGARLIPHYRIGAEISAVAPDKNTPIVLYCRSGRRSEIARQTLLELGYTQVV
                     DAGGYEQLKSDLAATGADSCSPTARC"
     gene            complement(988..1272)
                     /locus_tag="VIO83_08715"
     CDS             complement(988..1272)
                     /locus_tag="VIO83_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331391.1"
                     /translation="MKAWIVLPVLSLGLLLAGCAGKTQNRDGCAFQLDTAWQELDIAK
                     AEGFAGTVSYSKALSLLTAAKTLQQFESYDSCVRKAERARFYIRESRAGR"
     gene            complement(1350..2456)
                     /gene="mnmH"
                     /locus_tag="VIO83_08720"
     CDS             complement(1350..2456)
                     /gene="mnmH"
                     /locus_tag="VIO83_08720"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEY8331392.1"
                     /translation="MRANSAHYRDIFLHDLPLMDVRAPVEYTKGAFPHTVNLPLMNDL
                     ERQKVGTSYKQHGQDAAIELGHQLVRGQLKAERIEAWAAFARANPDGYLYCFRGGLRS
                     QITQQWLKEEAGIDYPRVIGGYKALRNFLIDTTEAAVAECDFVIVGGMTGTGKTEVLA
                     QLDNAVDLERHANHRGSSFGKHATPQPGQIDFENLLAIDLLKKRAAGMQQFVLEDESR
                     LIGICSLPLPLHHGMQQYPLVWLEDSLEGRIERILGDYVIDLSAEFIALHGEEQGFVA
                     FAERLQQSLKNIVKRLGGERYQRLAAIMDEALVEQQRSGAVDLHRGWIEGLLGEYYDP
                     MYAYQRESKAARIEFSGDQQAVVEYLRQRAVNRR"
     gene            complement(2456..3490)
                     /gene="selD"
                     /locus_tag="VIO83_08725"
     CDS             complement(2456..3490)
                     /gene="selD"
                     /locus_tag="VIO83_08725"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEY8331393.1"
                     /translation="MSEPIRLTQYSHGAGCGCKISPKVLEVILDGSGAQNLDPKLWVG
                     NASRDDAAVYAIDDERGVVSTTDFFMPIVDDPFDFGRIAATNAISDIYAMGGDPLMAI
                     AILGWPVNVLAPEVAREVIRGGRSVCDEAGIPLAGGHSIDAPEPIFGLAVTGIVDKRF
                     MKRNDTATEGCKLYLSKPLGIGILTTAEKKGKLRAEDVGLARDWMCTLNKPGARFGKL
                     EQVKAMTDVTGFGLLGHLVEMADGSGLSARIDYAAVPRIAGVDYYLDQGCVPGGTLRN
                     FDSYGERIAPLPEVQKLLLCDPQTSGGLLVAVEPAGEAEFLALAAELGLELAPIGALV
                     ARQNHAVEVL"
     gene            3687..4682
                     /locus_tag="VIO83_08730"
     CDS             3687..4682
                     /locus_tag="VIO83_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8331394.1"
                     /translation="MGKRVALVLGSGGARGYAHIGVIEELEARGYQISCIAGSSMGAV
                     IGGIYAAGKLPVYRRWVETLDYLDVLRLLDVSFSLGAIRGDRIFGRIRAFLGEINIED
                     LPIPYTAVATDLTNQQEVWFQEGCLHQAMRASAAIPSLFTPVVQGSRVLVDGGLLNPI
                     PIVPVVSSRCDLIVAVNVNASNQVQYELPVIERPPAMRNRLDQMMGSIGALGARLAFR
                     RRGDSELPALLATAEEFAPTGSPHGVASSKASHTHALENEAPDSVLEMINQSFDVMQN
                     SLAQYKIAGYPPDILVNVPKRVCRFFEFYKAPELIALGRRIASDSLDKYEEHHSS"
     gene            complement(4722..6281)
                     /locus_tag="VIO83_08735"
     CDS             complement(4722..6281)
                     /locus_tag="VIO83_08735"
                     /inference="COORDINATES: protein motif:HMM:NF017870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEY8331395.1"
                     /translation="MNKTRIALAVLAAASAVGTLAPWYTGMQLEEVLQTSIRQGNEQL
                     QESVPGTGVSMELVALERGFFASTAHYRLTLDDTSSGEEPTELLFSDRIEHGPLPLSR
                     LQALELKPVMAASRFELERSEDVIPWFVGAGGGSPLTGSLVLNYDNSVTGRVTLLPLQ
                     FAGEGNSVRFSGMNLDVAAGAQSEYLRLAGGMDSLILDLTRDGEPLQIQLKGLSLRSD
                     YRLGQSGLYTGDSRLALQQAQLLLADRPPVEVRDFVQTDRLEELDGQMKGAFGYDIGM
                     LSYGSQPLGSLNLSLSLANLDVLALKSLGDWYTGFLRRLQTQPDGDTGLSAEEHDGLQ
                     AGLGALLAGQPKLALDNLSLKTANGESRFNLVVDLARPVSYELPPAELARQLVGKVDA
                     RLVLAKPMIRDVVMYQAALDPAVDAAAAALEATQLAEMAGEMATSTQLARVEGDNIVA
                     NLSYADGQIELNGQTLPAEQFAGLLLSMAPAEAMGLMMGAEDGSAAGDGQDYPGADSG
                     EMATDSEAEPR"
     gene            complement(6330..7397)
                     /locus_tag="VIO83_08740"
     CDS             complement(6330..7397)
                     /locus_tag="VIO83_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8331396.1"
                     /translation="MIEHDRLLVQILMLSLLGACLWVLTPFWSALFWAAVLAFASWPL
                     MLGLTRLLRGRTAVAAGVLTLAWMLLVAGPLVWLGFNLADHIRSVVALLKDLQVEGLP
                     PPPTWLAGVPLFGARLVELWNSIDQQGTALFVTLKPYLGEMGNWLLARSAQLGKGVLE
                     LVLSLVLLFFFYRDGPRMAEYARSLLERLLDERADHYLELVAGTVQRVVNGVIGTAAA
                     QALLAYIGFLIAGVPGALILGLLTFAFSFIMIPPLVWGPATAWLFWQGDYGMGLFLGI
                     WGLVVISGVDNVLKPYLISRGGTLPLVVVLLGVLGGMLAFGFMGLFLGPTLLAVAYSL
                     IGDWIAGPQHKRRPAPPDAAA"
     gene            7534..>7720
                     /locus_tag="VIO83_08745"
     CDS             7534..>7720
                     /locus_tag="VIO83_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8331397.1"
                     /translation="MKTKCAYRYRFYPTPKQEILLARTFGCVRSVYNTILAWRTEAFQ
                     QEKTRIGYKEASARLTAL"
BASE COUNT         1299 a         2656 c         2538 g         1227 t
ORIGIN      
        1 cttggctcgc ggtggaggac cccgccgcgc cttggccggc gctgggcaag gccggcaaga
       61 gcgccggtcc cttctatctg gtgtggacca accccgaagc ggaacagatc ggccccgagc
      121 aatggccgtt ccgcatggtg cgcctgcgcg tactggcgcc ggtggccgaa cgcttccctg
      181 cgctgcgccc ggatccggcg ctggcagcaa gcgatccggt caacctcggc ttcgcccagt
      241 tccagaagca ttgcctggcc tgccaccgcc tgaataagca gggcgatgcc accttcggcc
      301 ccgacctcaa cctgccctac aacccgaccg aatacttgcg cgccgacctg ctgcgccgct
      361 acatccgcga cccgcaggcc ctgcgccact ggccgcaggc gcgcatgccc ggcttcagcg
      421 aagcggcgct gagtgaggca cagctcgacc agttgctggc ctatctgcgc cacatggccg
      481 ggcgcaaggt ggccgccgac tgacggcggc cacgcgccag tgcgccgcag cgacgtcggc
      541 cggcggctct gttagggtaa ggaatgatgt ctggaggagt gttttcgatg cgccgtctgc
      601 tgctcgccct gagcctgatc ctgccgttgt ccgcctgtca ggcggatacc ccgccgcaaa
      661 ccctggccgc gatccagaac ggctcgcatg tgctgatcga cgtgcgcacc ggcgaggagt
      721 tcgccgccgg ccacctgcaa ggcgcccgac tgatccccca ctaccgcatc ggtgcggaga
      781 tcagcgccgt cgcccccgac aagaacacgc ccatcgtgct ctactgccgc agcggccgcc
      841 gctcggaaat cgcccgccag acgctgctcg agctgggcta cacccaggtc gtcgatgccg
      901 gcggctacga gcagttgaag agtgacctgg cggcgaccgg cgccgactcc tgcagcccga
      961 ccgccaggtg ctgaagccgc cctgcgctca gcgcccggcg cgcgactcgc ggatatagaa
     1021 acgcgcccgc tcggccttgc gcacacagct gtcataggac tcgaactgct gcaaggtctt
     1081 agccgcggtc agcagggaca gcgccttgga gtagctgaca gtaccggcga agccttcggc
     1141 cttggcgata tccagctcct gccacgcagt atccagctgg aaggcgcagc cgtcgcggtt
     1201 ttgcgtcttg ccggcgcagc cggccagcag caggccaagg gacagaacgg gcagcacgat
     1261 ccatgccttc atctttgcat ctcctgcagc gggaattcct gcaagtctag ccattggccc
     1321 ggactatgcg tccgggccag gcacggatat cagcgacgat tgaccgcccg ctggcgcagg
     1381 tattccacca ccgcctgctg gtcgccactg aactcgatgc gcgccgcctt gctctcgcgc
     1441 tggtaggcgt acatcgggtc gtagtactcg ccgagcaggc cctcgatcca gccgcggtgc
     1501 aggtcgaccg cgccgctgcg ctgctgttcg accagtgcct catccatgat cgccgccaga
     1561 cgctggtagc gctcgccgcc caggcgcttg acgatgttct tcaggctctg ctgcagacgc
     1621 tcggcaaagg cgacaaaacc ctgctcctca ccgtgcaggg cgatgaattc ggcggacagg
     1681 tcgatcacgt agtcgccgag gatgcgctcg atgcgtcctt cgaggctgtc ctccagccag
     1741 accagcgggt actgctgcat gccatggtgc aaaggcagcg gcagcgagca gatcccgatc
     1801 aggcggcttt cgtcctcgag gacgaactgc tgcatgccgg cggcgcgctt cttcagcagg
     1861 tcgatggcca gcaggttctc gaagtcgatc tgccccggct gcggggtggc gtgcttgccg
     1921 aagctggagc cgcgatggtt ggcgtggcgc tcgaggtcga cggcgttgtc cagctgcgcc
     1981 agcacctcgg tcttgccggt gccggtcatg ccaccgacga tcacgaagtc gcactcggca
     2041 accgccgcct cggtggtgtc gatgaggaag ttgcgcagcg ccttgtagcc gccgatcacc
     2101 cgcgggtagt cgatgccggc ctcttccttg agccactgct gggtgatctg cgaacgcagg
     2161 ccgccgcgga agcagtacag gtagccgtcg gggttggcgc gggcgaaggc cgcccaggcc
     2221 tcgatgcgct cggccttgag ctggccacgc accagctggt ggcccaactc gatggcggca
     2281 tcctggccgt gctgcttgta gctggtaccg accttctgcc gctcgaggtc gttcatcagc
     2341 ggcaggttga ccgtgtgcgg gaaggcgccc ttggtgtatt ccaccggggc gcgcacgtcc
     2401 atcagcggca ggtcgtggag gaagatgtcg cgatagtggg cactgttggc acgcatcaga
     2461 gcacctctac cgcgtggttc tgtcgggcca ccagtgcgcc gatcggcgcc agctcgagac
     2521 ccagttcggc ggccagggcg aggaattccg cctcgccggc cggctccacc gccaccagca
     2581 ggccgccgct ggtctgcggg tcgcagagca gcagcttctg cacttccggc agcggggcga
     2641 tgcgctcgcc atagctgtcg aagttgcgca gcgtgccacc gggcacgcag ccctggtcga
     2701 ggtagtagtc gacgccggcg atgcgcggca ccgccgcata gtcgatgcgc gcgctcaggc
     2761 cgctgccgtc ggccatctcc accaggtggc cgagcaggcc gaagccggtg acgtcggtca
     2821 tcgccttgac ctgctccagc ttgccgaagc gggcgccggg cttgttcagg gtgcacatcc
     2881 agtcgcgggc caggcccacg tcctcggcgc gcagcttgcc cttcttctcg gcggtggtga
     2941 ggatgccgat gcccagcggc ttggacaggt acagcttgca gccttcggtg gcggtgtcgt
     3001 tgcgtttcat gaagcgcttg tcgacgatgc cggtgaccgc caggccgaag atcggctcgg
     3061 gggcgtcgat ggagtgaccg ccggccagcg ggatgcccgc ctcgtcgcac accgagcgac
     3121 cgccgcggat cacctcgcgg gccacttccg gcgccagcac gttgaccggc cagccgagga
     3181 tggcgatggc catcagcggg tcgccgccca tggcatagat gtcgctgatc gcgttggtcg
     3241 cggcgatgcg gccgaagtcg aagggatcgt cgacgattgg catgaagaag tcggtggtgg
     3301 agaccacccc gcgctcgtcg tcgatggcat agactgccgc gtcgtcacgc gaggcgttgc
     3361 cgacccacag cttggggtcg aggttctgcg cgccactgcc gtcgaggatc acctccagca
     3421 ccttggggga aatcttgcag ccgcagccgg cgccgtgact gtactgggtg aggcggatcg
     3481 gttcgctcat ggggcactcc ggggtattcg atggcggcga ttctagcaaa cgccacggca
     3541 caagacccac agcaccccgg atgacctgcg gaagggccat actgaaacaa agggtgtgaa
     3601 ggatacgacg gtgcatgacc gtcacacccg gccggcaggg cttgcaccgg ttggtccggc
     3661 cataggttga aaggggggaa gacatcatgg gcaaacgtgt cgcgctggtg ctcggctcgg
     3721 gcggcgctcg cggctacgcg catatcgggg tgatcgagga gctggaagcc cgcggctacc
     3781 agatctcctg catcgccggc agttcgatgg gcgcggtgat cggcggcatc tacgccgccg
     3841 gcaagctgcc ggtctatcgg cgctgggtgg aaaccctcga ctatctggac gtgctgcgcc
     3901 tgctcgacgt cagcttttcg ctgggggcga ttcgcggcga ccgcatcttc gggcgcatcc
     3961 gcgccttcct cggcgagatc aacatcgaag acctgccgat tccctacacc gccgtggcca
     4021 ccgacctgac caaccagcag gaggtgtggt tccaggaggg ttgcctgcac caggccatgc
     4081 gggcctcggc agcgattccc agcctgttta ccccggtggt gcagggctcg cgggtgctgg
     4141 tcgacggcgg cctgctcaac ccgatcccga tcgtgccggt ggtctccagc cgctgcgacc
     4201 tgatcgtcgc ggtcaacgtc aacgccagca accaggtgca gtacgaactg ccggtgatcg
     4261 agcgtccacc ggccatgcgc aaccgcctcg accagatgat ggggtccatc ggcgcactgg
     4321 gcgcgcgcct ggcgtttcgc cgccgcggcg acagcgagct gcccgccctg ctggccaccg
     4381 cggaggagtt tgcgccgacc ggctcgcccc atggtgtcgc ctcgtccaag gccagccata
     4441 cccatgcgct ggaaaacgag gcgccggaca gcgtgctgga gatgatcaac cagagcttcg
     4501 acgtgatgca gaactcgctg gcgcaataca agatcgccgg ctacccgccg gacatcctgg
     4561 tcaacgtacc gaaacgggtg tgtcgcttct tcgagttcta caaggccccg gagctgatcg
     4621 ccctcggccg gcggatcgcc agcgactcgc tggacaagta cgaggagcac cactccagct
     4681 gaaacgacgg cacgggcggc ttgcggcgtc ccgcgccctg ctcagcgcgg ttcggcctcg
     4741 ctgtccgtgg ccatctcccc gctgtctgca cccgggtagt cctgcccatc gccagcggcg
     4801 ctgccgtcct ccgcgcccat catcaggccc atggcctcgg ctggcgccat ggacaacagc
     4861 aggccggcaa actgctcggc cggcagggtc tgaccattga gttcgatctg gccgtcggca
     4921 taactcaggt tggcgacaat gttgtcgccc tccacccggg caagctgcgt ggaggtcgcc
     4981 atctcgccgg ccatttccgc cagctgcgtg gcttccaggg cagccgccgc cgcgtcgacg
     5041 gccggatcca gggcggcctg gtacatcacc acatcgcgga tcatcggctt ggccagcacc
     5101 aggcgcgcat ccaccttgcc gaccagttgc cgggccagct ccgccggcgg cagctcatag
     5161 gacaccggcc gagccaggtc gaccaccagg ttgaagcggc tctcgccgtt ggcggtcttc
     5221 agcgacaggt tgtccagcgc cagtttcggc tggcccgcca gcagcgcacc gaggcccgcc
     5281 tgcagaccgt cgtgctcctc cgccgacagg ccggtgtcgc cgtccggctg ggtctgcagg
     5341 cggcgcagga agcccgtgta ccagtcgccg aggctcttca gcgccagcac atccagattg
     5401 gccagactca ggctcaggtt gagcgacccc agcggctggc tgccgtagct gagcatgccg
     5461 atgtcgtagc cgaaggcgcc cttcatctgc ccatccagct cctcgaggcg gtcagtctgc
     5521 acgaagtcgc gcacctcgac cggcggccgg tcggccagca gcagctgcgc ctgctgcagc
     5581 gccagacgac tgtcgccggt gtacagcccg gactggccga gacgatagtc gctgcgcagg
     5641 ctcaggccct tgagctggat ctgcagcggc tcgccgtcgc gggtcaggtc caggatcagg
     5701 ctgtccatgc cgccggcgag ccggaggtac tcggactgcg ccccggctgc cacatcgagg
     5761 ttcattccgg agaaacgcac gctgttgccc tccccggcaa attgcagggg aagcagcgtc
     5821 acccggcccg tgacgctgtt gtcgtagttc agcaccaggc tgccggtcag cggcgagccg
     5881 ccgccggcgc cgacgaacca ggggatgacg tcctcgctgc gctccagctc gaagcggctg
     5941 gccgccatca ccggcttcag ctccagggcc tgcaggcgcg acagcggcag gggaccatgt
     6001 tcgatgcggt cgctgaacag cagttcggtc ggctcctcgc cgctcgacgt gtcgtccagc
     6061 gtgagccggt agtgagcggt actggcgaaa aagccgcgct ccagagccac cagctccatg
     6121 ctgacgccgg tgccgggtac ggactcctgc agctgttcgt tgccctggcg gatggaggtt
     6181 tgcagtacct cctccagttg catgccggta taccaggggg caagcgtgcc aacagcgcta
     6241 gcggcagcca acacggccag ggcgatcctt gtcttgttca aattgactct tccagttgag
     6301 taagggggga cactggcccc cggtgcggct caggccgctg cgtcgggcgg cgccggccgc
     6361 cgtttgtgct gcgggccggc gatccagtcg ccgatcaggc tgtaggccac cgccagcagg
     6421 gtcggaccga ggaacagccc catgaacccg aaggccagca tgccaccgag cacgccgagc
     6481 agcaccacca ccagcggcag ggtgccaccg cggctgatca ggtagggctt gagcacgttg
     6541 tccacgccgc tgatgaccac cagcccccag atgccgagga acaggcccat gccatagtcg
     6601 ccctgccaga acagccaggc ggtagccggg ccccagacca gtggcggaat catgatgaaa
     6661 ctgaaggcaa acgtcagcag cccgaggatc agggcgcccg gcacaccggc gatgagaaaa
     6721 ccaatgtagg ccagcagtgc ctgcgccgcc gcggtgccga tcacgccgtt gaccacccgc
     6781 tgcacggtgc cggccaccag ctccagatag tgatcggcac gctcgtccaa cagacgctcc
     6841 agcaggctac gggcgtactc cgccatccgc gggccgtcgc ggtagaagaa gaacagcaac
     6901 accaggctca gcaccagctc cagcacaccc ttgcccagct gggcgctgcg cgccagcagc
     6961 cagttgccca tctcgcccag atacggcttg agcgtgacga acaaggccgt gccctgctgg
     7021 tcgatggaat tccacagctc caccaaccgg gcgccgaaca gcggcacccc ggccagccag
     7081 gtcggcggcg gcggcaggcc ttcgacctgc agatccttga gcagggctac caccgagcgg
     7141 atatggtccg ccaggttgaa gccgagccag accagcggcc ccgccaccag cagcatccag
     7201 gccagggtga gcaccccggc ggcgacagca gtgcgcccac gcagcaggcg ggtcagaccc
     7261 agcatcagcg gccagctggc gaaggccagt accgcggccc agaacagcgc cgaccagaac
     7321 ggcgtcagca cccacaggca ggcaccgagc agggacagca tcagaatctg caccagcaga
     7381 cggtcgtgct cgatcatcag atttgcgcga ggaacctcgg cattcatgcc ggggagggat
     7441 agcgcggcgc ccgagggcgc cccagttctc gcttctcctt agatagggct atcttgggtt
     7501 gatccttctg ctggatatgc atacagttaa agcatgaaga cgaaatgcgc ctaccgctac
     7561 cggttctatc cgacgcccaa gcaggaaatc ctgctggcgc ggacgttcgg ctgcgtgcgc
     7621 tccgtctaca acacgatcct ggcttggcgc accgaggcgt ttcagcaaga gaaaacacgc
     7681 ataggctaca aggaagccag cgcccggttg accgcgctga
//
LOCUS       DATCUN010000228         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_179436, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000228 DATCUN010000000
VERSION     DATCUN010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179436"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VIO83_08750"
     CDS             complement(<1..201)
                     /locus_tag="VIO83_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8331398.1"
                     /translation="MPLPQLRHWVFDMDGTLTVAVHDFAAIRRALEIPPQDDILAHLA
                     ALPAELAAAKHAWLLAHEQTLAQ"
     gene            complement(227..1102)
                     /locus_tag="VIO83_08755"
     CDS             complement(227..1102)
                     /locus_tag="VIO83_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEY8331399.1"
                     /translation="MTRVLDELVALLSLETIEENLFRGVSQDLGLRQLFGGQVIGQSL
                     SAASQTVPAERHMHSMHAYFLRPGDATMPVLYQVDRVRDGGSFNTRRVLAIQKGRPIY
                     SCSVSFQRDEGGLHHQIEQMPDVPGPEGLLSEHELHRQRLAQQPDGGRERAYFDIPIE
                     VRPVNPGIASQPQPCEPVKYLWVRAAGQLPDVPALHKYLMAYATDFGLLTTALLPHGV
                     SMWDRSMQIASLDHSLWFHRNDVRVDEWLLFALDSPWAGHGRGFTRGSIFNRAGELVA
                     SAVQEGLIRQRSDWA"
     gene            complement(1138..>1706)
                     /locus_tag="VIO83_08760"
     CDS             complement(1138..>1706)
                     /locus_tag="VIO83_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY8331400.1"
                     /translation="NSPLPLGLSGQPPRVDLLLFVHRVQGLAPGLYWLARGDQAPETL
                     KAGLRDDFLWQRADAELPLYRLLAGDARGLAQFLACDQDIASDGCVAFAMLARFDQAL
                     AAGAWQYAELYRECGRIGQLLYLEAEAAGLSGTGIGCFFDPAVHELLGMADSRLQSLY
                     HFTIGKAQVDARLSSQPAYPHLRWPPPR"
BASE COUNT          276 a          575 c          617 g          238 t
ORIGIN      
        1 ctgcgccagc gtctgctcgt gggccagcag ccaggcgtgc ttggccgccg ccagctcggc
       61 cggcagggcg gcgagatggg cgaggatgtc gtcctgcggc ggaatctcca gcgcccggcg
      121 gatcgccgcg aagtcgtgca cggcgacggt cagcgtgccg tccatgtcga acacccagtg
      181 gcgcaactgc ggcaacggca gccgctcgat caacccgccc atggcgtcac gcccagtccg
      241 agcgctggcg gatcagccct tcctggaccg ccgaggcgac cagctcgccg gcgcggttga
      301 agatgctgcc gcgggtgaag ccgcggccgt ggccggccca cgggctgtcg agggcgaaca
      361 gcagccactc gtcgacccgc acgtcgttgc ggtggaacca cagcgagtgg tcgaggctgg
      421 cgatctgcat cgagcgatcc cacatcgaca cgccatgcgg cagcagcgcg gtggtcagca
      481 ggccgaagtc ggtggcgtag gccatcagat acttgtgcag cgcaggaaca tccggcagct
      541 ggccggcggc acgcacccac aggtacttga ccggctcgca cggctgcggc tggctggcaa
      601 tgcccggatt gaccgggcgg acctcgatcg gaatgtcgaa gtaggcgcgc tcgcgcccgc
      661 cgtcaggctg ctgagcgagg cgctgacggt gcagttcgtg ctcggacagc aggccttccg
      721 ggccgggcac gtcgggcatc tgctcgatct ggtggtgaag gccgccctcg tcgcgctgga
      781 aggacacgct gcagctgtag atcggccggc ccttctggat cgccagcacg cggcgggtgt
      841 tgaagctgcc gccgtcgcgc acgcggtcga cctggtagag caccggcatg gtcgcatcgc
      901 ccgggcgcag gaagtaggca tgcatcgagt gcatgtggcg ctcggccggc accgtctggc
      961 tggccgccga cagcgactgg ccgatcacct ggccgccgaa cagctggcgc aggccgagat
     1021 cctggctgac gccgcgaaac aggttttcct cgatggtttc cagactgagc agggctacca
     1081 gctcatcgag tacgcgggtc atgaaaatct ccttgcaaca cgaagccggg ccgccgctca
     1141 acggggcggc ggccagcgta aatgggggta ggccggctga ctgctcagac gagcgtccac
     1201 ctgcgccttg ccgatggtga aatggtaaag actttgcagg cggctgtcgg ccattcccag
     1261 caattcgtgc actgcaggat cgaaaaagca gccgatgccg gtgccggaca gcccggccgc
     1321 ctcggcctcc aggtagagca gctggccgat gcgcccgcac tcgcggtaca gctcggcgta
     1381 ctgccaggcg ccggcggcca gggcctggtc gaagcgcgcc agcatggcga aggccacgca
     1441 gccgtcgctg gcgatgtcct ggtcgcaggc gaggaactgc gccaggccgc gggcatcgcc
     1501 cgcgagcagg cggtacagcg gcagctcggc atcggcgcgc tgccagagga agtcgtcgcg
     1561 cagaccggcc ttcagtgtct cgggcgcttg gtcgccgcgc gccagccagt acaggccggg
     1621 cgccagcccc tgcacgcggt ggacgaacag cagcaggtcg acccgcggcg gctggccgct
     1681 cagccccagc ggcagcggcg agttgc
//
LOCUS       DATCUN010000229         3643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_122522, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000229 DATCUN010000000
VERSION     DATCUN010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122522"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="VIO83_08765"
     CDS             <1..1297
                     /locus_tag="VIO83_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HEY8331401.1"
                     /translation="DDMGGTTSVWRLYEGHTSPLLRSFLSPVTVTADLADAGKTYDGE
                     IASGTTTYTLDPGVDTGLLLGGDTLAYATSSGNAGVYSATDGSLSLGEGLYSTQQGYD
                     LRYEGSLSIGKAGLTITANDASKTYGQSASLSGYTASGLVNGDTVSSVDLSSAGAAST
                     ATVGNYTITAANADGTGLSNYAISYVDGTLTVGKAGLTITASDASKTYGQSASLTGYT
                     ASGLVNGDTVSSVDLSSAGAASTATVGNYTITAANADGTGLSNYAISYVDGTLTVGKA
                     GLTITANDASKTYDGLAWSGGNGVSYSGWVNGEDASVLRGTLVYGGTSQGAIAPGSYS
                     ITASGLISDNYAIDYIDGLLSIQAAPAEEWTRQLPPGYAAALASLHRGERHAADPADS
                     TGTGASYEVIADGIRLPWSCIADEQVPAGAQDCAKRGTR"
     gene            1294..2964
                     /locus_tag="VIO83_08770"
     CDS             1294..2964
                     /locus_tag="VIO83_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEY8331402.1"
                     /translation="MKRLCFCLLSGVAALQAHAQQVPVQNPVVPDAGRSMRELEQRPP
                     SLPQKQSLDLDLPDAPLQQEAQSSQRVWVDGFVLEGNSAIPSTELLPLLDDLVHRQVS
                     LGQLQAGAHRLTQHYRARGYPLARAYLPAQAIDQGQVRIAVLEGRYGQVQVHNTSRLR
                     QAAVAAPLRTLKPGEAVRVAPLERALLSLRDLAGVQAKATLQPGASVGATDLVVEVQP
                     EPMLSGTIEADNFGNRYTGEYRLGGSLDINSPLRLGDRLSLRALGTSEDQHYYRAGWQ
                     VPVGPWSTRLGLAWSRMDYELGKDFADLRGHGLARIASMYVSQPLLRRRDAALYVTLQ
                     YDHKNLRDDIDLYDIRSRKRSRVWTTTLRGDERDGWLGGGANSFALSWSHGQLDIEDP
                     EGRVLDAATTDAAGNFNVIRPSLVRLQRIAGRFSVYAQLQGQWSDRNLDSSEKFYLGG
                     AYGVRAYPQAEAAGDQGWLANLEVRQALTEAWRMSVFIDHGQVRLSKDPWDDGGNHRS
                     LSGIGLGTDWAAHGWRLGASASWKLGSAEAQDGHAREPQIWAQVAFDF"
     gene            complement(3126..>3643)
                     /locus_tag="VIO83_08775"
     CDS             complement(3126..>3643)
                     /locus_tag="VIO83_08775"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8331403.1"
                     /translation="DGQSRAYHGVDIDAGKIQRARRIARRTGLADAHFEVLDLAARWP
                     EHAGNIAILDVLQYLPDHVQGGLLTHMAHMLAPGARLVIRSALGDDSGRGRTTRVTDL
                     IAHLAGWMQEVPKSYPTRETLQRHLSAAGLTATFSPLYGNTPFNNWLIVAERAQKHAR
                     QAPGGRHAPGA"
BASE COUNT          637 a         1170 c         1240 g          596 t
ORIGIN      
        1 cgatgacatg ggtggcacca ccagcgtctg gcgcctctat gagggccata ccagcccgct
       61 gctgcgcagc ttccttagcc cggtgaccgt gactgccgac ctggctgatg ccggcaagac
      121 ctacgatggc gagattgcca gtgggacgac gacctacact ctcgacccgg gcgtggacac
      181 cggcctgctg ctcgggggcg acaccctcgc ctacgccacg agcagcggca atgcaggcgt
      241 ctatagcgcc accgacggca gcctgagcct cggggaaggg ctgtattcga ctcagcaagg
      301 ctatgacctc cgctacgaag gcagtctgag catcggcaag gccggtctga cgatcactgc
      361 caacgatgcc tccaagacct acggccagag cgcgagcctg agtggctata cggcttcggg
      421 cctggtcaat ggcgatacgg tgagcagcgt ggatctgtcc agcgccggtg ctgcttcgac
      481 cgccacggtg ggtaactaca ccatcaccgc ggccaacgcc gatggcaccg ggctgtcgaa
      541 ctacgccatc agctatgtcg acggcaccct gaccgtgggc aaggccggtc tgacgatcac
      601 cgccagcgat gcctccaaga cctacggcca gagcgcgagc ctcaccggct acacggcttc
      661 gggcctggtc aatggcgata cggtgagcag cgtggatctg tccagcgccg gtgctgcttc
      721 gaccgccacg gtgggcaact acaccatcac cgcggccaac gccgatggca ccgggctgtc
      781 gaactacgcc atcagctatg tcgacggcac cctgaccgtg ggcaaggccg gtctgacgat
      841 caccgccaac gatgcctcca agacctatga cggcctggcc tggagtggcg gcaacggggt
      901 gagctacagc ggctgggtca acggagagga cgcctcggtg ctccgcggaa cgctggtcta
      961 cggcggaact tcgcaggggg cgatcgcccc tggcagttac tcgatcaccg ccagtggtct
     1021 gatctcggac aactatgcca tagattacat cgatggcttg ttgagcatcc aggctgctcc
     1081 ggccgaggaa tggacgcggc agttgccgcc gggctatgcc gctgcgctgg cgtcgctgca
     1141 tcggggcgaa cgtcatgcag ccgatccggc ggatagcacc ggcactggtg cctcgtatga
     1201 agtgatcgcc gacggtattc gtctgccttg gtcttgtatc gctgatgaac aggtgccggc
     1261 tggagcccag gactgcgcca aacgaggaac acgatgaaac ggctctgttt ttgcctgctc
     1321 agcggcgtcg ctgcgcttca ggcccatgcc cagcaagtgc cggtgcagaa ccccgtggtg
     1381 cctgacgccg gccgctcgat gcgcgaacta gagcagcggc caccgtcgct gccgcagaaa
     1441 cagtcactcg atctggacct gccggacgcg ccgttgcagc aggaggcgca gagcagccaa
     1501 cgggtgtggg tggacggctt cgtcctcgaa ggcaacagcg cgatcccgag cacagagctg
     1561 ctgccgctac tggacgacct ggtgcaccgg caggtctcac tgggccagtt gcaggccggc
     1621 gcccatcgcc ttacccagca ctaccgcgcg cgcggatatc cgctcgcccg cgcctacttg
     1681 cctgcccaag caatcgatca gggccaggtc aggatcgcgg tgctggaagg gcgctacggc
     1741 caggtccagg tccacaacac ctcgcgcctg cgccaagccg cagtggcagc acccctgcgc
     1801 acgctgaagc cgggcgaagc ggtgcgtgtt gcaccgctgg aacgggccct tttgtccttg
     1861 cgcgacttgg caggcgtgca ggccaaggcc acgctacagc caggcgccag tgtcggcgca
     1921 accgacctgg tcgttgaggt acagccggag ccgatgctga gcggcacgat cgaggcggac
     1981 aatttcggca atcgctacac cggtgaatat cgcctgggcg gcagcctgga catcaacagc
     2041 ccgctgcgcc tcggcgatcg attgagcctg cgcgcactgg gcacgagcga ggatcagcac
     2101 tactaccggg cgggctggca agtgccggtc ggaccctggt ccacccgcct cggactggcg
     2161 tggtcacgaa tggactacga attgggcaag gacttcgccg atctgcgcgg ccacggcctg
     2221 gcgcgcattg ccagcatgta cgtctcccag ccgctgctgc gcagacgcga tgccgcgctc
     2281 tacgtcacgc tgcagtacga ccacaagaac ttgcgcgacg acatagacct gtacgacatc
     2341 cgcagcagga agcgttcgcg cgtatggacc acgaccttgc gtggggacga gcgcgacggt
     2401 tggttgggcg gcggcgcaaa cagcttcgcc ttgtcctgga gccatggtca gctggacatc
     2461 gaggaccccg aagggcgggt gctggacgcg gccaccaccg acgcggccgg caatttcaac
     2521 gtgatacgtc caagcctggt gcggctgcaa cgcattgctg gccgtttcag cgtgtacgcg
     2581 caattgcagg gccagtggag tgaccgcaac ctggacagct cggagaaatt ctatctgggc
     2641 ggcgcctatg gcgtgcgtgc ttatcctcag gcagaggcgg ccggcgacca gggttggctg
     2701 gccaacctag aggtgcgcca ggccctgacc gaggcttggc gaatgtcggt cttcatcgat
     2761 cacggacagg tgcgcctcag caaagaccca tgggacgacg gtggcaacca ccgcagtctt
     2821 tcaggcattg gcctgggcac tgactgggct gcgcacgggt ggcggctcgg cgcctcggcg
     2881 tcctggaagc tgggcagtgc cgaggctcaa gacgggcacg cgcgcgagcc gcagatatgg
     2941 gcgcaagtcg cgttcgattt ttgattgccc atggcagacc tatcgcaggc ggatcagcca
     3001 aggcgcaagt gtgcaggggc atgtcgtcgg cccggcgccg attgtcaggg agcgcaggcg
     3061 gcagaagccg ggcaggttgc caatcgcgca gagcgcccta caggcggtga gctttcgcct
     3121 cgaagtcacg cgcctggagc gtgtctaccg ccaggcgcct gacgcgcgtg tttttgcgcc
     3181 cgctcggcca cgatcaacca gttgttgaac ggcgtgttgc cgtacagcgg cgagaaggtc
     3241 gcggtcaggc cggcggcgct tagatggcgt tgcagggttt cgcgggtcgg gtagctcttg
     3301 gggacttcct gcatccatcc ggccaggtgt gcaatcaggt cggtgacccg ggtggtgcgg
     3361 ccgcggccgc tgtcatcgcc cagcgcgctg cggatcacca gcctggcgcc gggggcgagc
     3421 atgtgcgcca tgtgggtcag caggccgccc tgcacgtggt cgggcaggta ctgcagcacg
     3481 tccaggatgg cgatgttgcc ggcgtgctcg ggccagcgcg cggccaggtc gagcacttcg
     3541 aaatgcgcat cggccaggcc ggtgcggcgt gcgatccggc gggcgcgttg gatcttgccg
     3601 gcgtcgatgt ccacgccgtg ataggcgcgc gactggccat cgg
//
LOCUS       DATCUN010000230         6315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_160124, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000230 DATCUN010000000
VERSION     DATCUN010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160124"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VIO83_08780"
     CDS             complement(<1..503)
                     /locus_tag="VIO83_08780"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HEY8331404.1"
                     /translation="MSSALASLLELPSAEAFDRWLAEPGEWVEEPNERRGGMSGVQRM
                     RTSDGRLLYRKQQIDHCYRDWQHPFGEPTVLREQRALEAFAALGVGVPQVMFCATRRQ
                     QGRWQALLVTAALDGFDSLEAIYARGEPQAWSESLRQRIFQQLGNLLARLHQARWQHG
                     CLYPKHI"
     gene            complement(507..1187)
                     /locus_tag="VIO83_08785"
     CDS             complement(507..1187)
                     /locus_tag="VIO83_08785"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8331405.1"
                     /translation="MTKPVELEFSRKYDAEHAQQYFEKHQDGLSRRASHWRDVQLARR
                     ALQRAGDPGLVLDLPCGAGRFWPLLAEKANRVIIGADNSEHMLNIALGSQPADVVKRV
                     KPLQTSAFAIDLPDNAVDNIFSMRLLHHIGEAKDRAVLLREFHRVTRDSVILSLWVDG
                     NFKSWRRSRLEKRRLARGKVGTYQNRFVAQREQIEREFKAAGFRIDTHYDFVPGYAMW
                     RVYVLRKA"
     gene            complement(1269..2543)
                     /locus_tag="VIO83_08790"
     CDS             complement(1269..2543)
                     /locus_tag="VIO83_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017529272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8331406.1"
                     /translation="MAFRQSLSKRIVTAFVLMTLLVAGAFALGTVVTVHVVEQRLLSA
                     ELGRDLDRLLRMENMDEWRLQPQPDQLFYVSDGPVELAMTEDVAGLSEGFHEVFRGPQ
                     SFHAMVREVGGRHYVLLQDQSDFEAREELMYVVVTVGFIFSLLLAAALGWLLARQVMA
                     PVTRLAHQVRHRDQLLALAPPLAPDYAPDEVGQLAGAFDETLNQLREVLNREQLFTSD
                     VSHELRTPLMVLASSCELLLENPALDERARRQVQRIARASSDMRELVQTFLLLARAQS
                     GQASLAQQATLAQVADELVAQWRGPIEAKGLRLDYLTDHPQSTLYNAPFLRSVLGNLL
                     RNAWHYTDSGHIRLALGAAGFVVEDSGEGIPEALRESVFEPFVRGNRERGDGLGLGLS
                     LVKRICEHEGWQIRLQSAQPHGCRFEVRLTPG"
     gene            complement(2533..3216)
                     /locus_tag="VIO83_08795"
     CDS             complement(2533..3216)
                     /locus_tag="VIO83_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8331407.1"
                     /translation="MRILLVEDNRDILANLADYLTLKGYTVDCAQDGLSGLHLATSGH
                     YDLLVLDVMLPGLDGFSLCQRLRDEARSDIPVIMLTARDQLDDRLQGFRAGADDYLVK
                     PFALSELAARIEAVLRRTQGGGRRLLQVADLDYDLDTLEVSRAGRRLKLNPLGLKLLA
                     LLMQKSPHVLRREALEAALWGDNCPDSDSLRSHIHQLRQVIDKPFPRPLLHTVHGLGY
                     RLAESDDGV"
     gene            3384..5363
                     /locus_tag="VIO83_08800"
     CDS             3384..5363
                     /locus_tag="VIO83_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEY8331408.1"
                     /translation="MPLLRSAPLRYLLLVLACWLGLFALTRSVLLIGHWSEAAVALSD
                     LPALFLTGLAYDLGFLAYAAVPLGLYLLLCPAGLWRRPGHRLWLHGLLAVSLFAMLFS
                     ALSEWLFWDEFGVRFNFIAVDYLVYSEEVIHNILESYPVFRLLGAIAVLAGGLTLALR
                     PLVRRALDAELPPLRQRLAGFALLAAGCGLGFALLDQQHPRGLGGNAYQHELASNGPY
                     QFFAAFRNNELDYQQFYPTLEMTKVAGILREELQEPNARFNDGEPLNIRRQIDNPGPA
                     SKLNVVLVTIESLSAKYLGSFGDSRGLTPNLDELRQHSLFFNNVYATGTRTDRGLEAI
                     TLSIPPTPGRSIVKRIGRESGFASLGQQLAGQGYDSVFVYGGRGYFDNMNAFFSGNGY
                     RIVDQSSVPEQDIQFANAWGMADEDLYAQAMKVADADHAAGKPFLLQLMTTSNHRPYT
                     YPDGRIDIASGDGREGAVKYTDWAIGDFLAKARQKPWFDDTVFIFVADHCAGSAGMED
                     LPVANYHIPLFVYAPKWIQAREDGQLASQIDLAPTLLGLLGADYESTFFGRDLLQPHR
                     NPPRVLIGNYQHLGLFDGRNLAILSPNRLMRRHDDALGASREHTANRDDALIDRDIAY
                     YQSASYAFSNGLLTWRGQAPATTTFGLAGQTERAN"
     gene            5363..6112
                     /locus_tag="VIO83_08805"
     CDS             5363..6112
                     /locus_tag="VIO83_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY8331409.1"
                     /translation="MNLSIQGASRPLRTWLALGLPLALIVLLLVFEPTRLDFALSDLF
                     YQPGVGFSGGHSAFLEDFLHDRAKQGVIVIGVLAIAGLLSSWLYRPWAALRRPFGYLV
                     LAMALSTAIVNPVKTLTGVHCPWSLTQYGGEETYSPLLGERAPVADKAGRCWPGGHAS
                     SGFTLFALYFVLRDRKPRLARAALLFALGLGTVFSMGRVMQGAHFLSHNVWTALFDWM
                     ICLGFYRLILYRQAQPAVAPALATRRLESAD"
     gene            complement(6129..>6315)
                     /locus_tag="VIO83_08810"
     CDS             complement(6129..>6315)
                     /locus_tag="VIO83_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY8331410.1"
                     /translation="LYMATLTATRHNPAIAAFYARLLAAGKLPKVALVACMRKLLTTL
                     NAIARSGLPWNPEIKTT"
BASE COUNT         1052 a         2218 c         2000 g         1045 t
ORIGIN      
        1 aagatgtgct tgggatacag gcagccatgc tgccagcgag cctggtgcag acgcgccagg
       61 aggttgccca gctgctggaa gatgcgctgg cgcagcgact cgctccaggc ctgcggctcg
      121 ccgcgggcat agatcgcttc caggctgtcg aaaccatcca gcgccgccgt caccagcaac
      181 gcctgccagc gcccctgctg acgccgggtc gcgcagaaca tcacctgcgg cacgcccaca
      241 ccgagcgcgg cgaaggcctc cagggcacgc tgctcgcgca gcacggtcgg ctcgccgaac
      301 gggtgctgcc agtcgcggta gcagtgatcg atctgttgct tgcgatacaa caggcggcca
      361 tcgctggtac gcatgcgctg caccccgctc atgccaccgc gacgctcgtt gggctcctcg
      421 acccactcac ccggctccgc cagccaacgg tcgaaagcct cggccgacgg cagttccagc
      481 aggctagcca atgcactgct catggctcag gccttgcgca gcacgtagac ccgccacatg
      541 gcgtaacccg gaacgaagtc gtaatgggtg tcgatgcgga aaccggcggc cttgaactcg
      601 cgctcgatct gctcgcgctg ggcaacgaag cggttctgat aggtgccaac cttgccgcgg
      661 gccagacgac gcttctccag gcggctgcga cgccaggact tgaagttgcc atccacccac
      721 agcgagagga tcacgctatc gcgggtcacc cggtggaact cgcgcagcag gaccgcgcgg
      781 tccttggctt cgccgatgtg gtgcagcagg cgcatggaga agatgttgtc gacggcattg
      841 tccggcagat cgatggcgaa tgcggaagtc tgcagcggct tgacccgctt gaccacatcg
      901 gctggctgcg agccaagggc gatgttgagc atgtgctcgg agttgtcggc gccgatgatc
      961 acccggttgg ccttttccgc cagcagcggc cagaagcgcc cggcaccgca gggcaggtcg
     1021 agcaccagtc ccggatcgcc ggcgcgctgc agggcccggc gggccagctg cacgtcgcgc
     1081 cagtgcgacg cacgccgcga caggccatcc tggtgcttct cgaaatactg ctgggcgtgc
     1141 tcggcgtcgt atttgcggga aaactcgagc tcgacaggct tggtcattga aggacttcca
     1201 acggttgttg ataggccaga ttatctgccc aagcgtgata ttcgagtcaa acaaatgtga
     1261 aaatttcgtc aaccaggagt caggcgcacc tcgaaacgac agccatgcgg ctgtgccgac
     1321 tgcagacgaa tctgccagcc ctcatgctcg cagatccgct tgaccagcga cagccccagc
     1381 cccagaccgt cgccgcgttc gcggttgccg cgcacgaacg gctcgaatac cgactcgcgc
     1441 agggcttcgg gaataccctc gccgctgtcc tcgacgacga agcccgcggc gcccagggcc
     1501 aggcggatat ggccgctgtc ggtgtagtgc caggcgttgc gcaacaggtt gcccagcacc
     1561 gagcggagga acggggcgtt gtacaaggtc gactgcggat gatcggtgag gtagtcgaga
     1621 cgcagcccct tggcctcgat cggcccgcgc cactgcgcca ccagctcgtc ggcgacctgc
     1681 gccagggtgg cctgctgcgc caggctggct tggccggact gggcccgcgc cagcaacagg
     1741 aaggtctgca ccagttcgcg catgtcgctg ctggcgcggg caatgcgctg cacctggcga
     1801 cgtgcgcgct cgtccagcgc cgggttctcc agcagcagct cgcaggagct ggccagcacc
     1861 atcagcggcg tacgcagttc atggctgacg tcgctggtga acagctgctc gcgattgagc
     1921 acctcgcgca gctggttgag ggtctcgtcg aaggccccgg ccagctggcc aacctcgtcg
     1981 ggtgcgtaat ccggggccag cggcggcgcc agcgccagca gctggtcacg gtgacggacc
     2041 tgatgcgcca agcgcgtcac cggcgccatc acctggcgcg ccagcagcca gccgagcgcg
     2101 gcggccagca gcaggctgaa gatgaagccc accgtcacca cgacatacat cagttcctcg
     2161 cgcgcctcga aatcgctctg atcctgcagc agcacatagt ggcgcccgcc cacctcgcgc
     2221 accatcgcgt ggaacgactg cggcccgcgg aacacctcgt ggaacccctc gctcagccct
     2281 gccacatcct cggtcatggc caactcgacg gggccgtcgc tgacatagaa cagctgatcc
     2341 ggttgcggct gcaggcgcca ctcgtccatg ttctccatcc gcagcaggcg gtcgagatcg
     2401 cgaccgagct cggcggacag caagcgctgc tcgaccacgt gcaccgtcac caccgtgccc
     2461 agcgcgaaag cgccagccac cagcagagtc atcagcacga aagcggtgac gatccgtttc
     2521 gacaggctct gcctaaacgc catcgtcgct ctccgccaga cgatagccca ggccatgcac
     2581 ggtgtgcagc agcgggcggg ggaacggctt gtcgatcacc tggcgcagct ggtggatgtg
     2641 gctgcgcaga ctgtcgctgt ccggacagtt gtcgccccac agcgccgcct cgagcgcctc
     2701 gcggcgcagc acgtgcgggc ttttctgcat cagcagggcc agcagcttga gccccagcgg
     2761 gttgagcttg aggcggcgac cggcgcggct gacttccagg gtgtcgagat cgtagtcgag
     2821 gtcggccacc tgcagcaggc gccgaccgcc gccctgggtg cggcgcagca ccgcctcgat
     2881 gcgcgccgcc agttcggaca gcgcaaacgg cttgaccaga tagtcgtcgg cgccggcccg
     2941 gaacccctgc aggcgatcgt cgagctggtc gcgggcggtg agcatgatca ccgggatatc
     3001 gctgcgcgcc tcgtcacgca ggcgctggca caggctgaag ccgtccaggc cgggcaacat
     3061 cacgtcgagc accagcaggt cgtagtggcc actggtggcc agatgcaggc cggacaggcc
     3121 gtcctgcgca cagtcgaccg tgtagccctt gagcgtcagg tagtcggcca ggttggccag
     3181 gatgtcacga ttgtcttcga ccagcagaat acgcatgcga gcacctcctg ccgtcatctt
     3241 gcggcggcag gtttaggaga ttcttaagtg ctgcaaaggt actcaaccac ggcggggttt
     3301 gacacatttt tcactgcggg ttcacaaggc cgcgacggtg ccgggagcaa gctgcgcgca
     3361 ccgaactgcc tggacccctc gtcatgccct tgttgcgctc cgctccactg cgttatctgt
     3421 tactcgtcct agcctgctgg ctcggccttt tcgccctgac ccgcagcgta ttgctgatcg
     3481 gccactggtc ggaagccgca gtggcgctga gcgacctgcc cgccctgttc ctgaccggcc
     3541 tggcctacga cctcggcttc ctcgcctacg ccgccgtgcc gctgggcctg tacctgctgc
     3601 tctgccccgc cggcctgtgg cgccgtcctg gccaccggct gtggctgcac ggcctgctcg
     3661 ccgtcagcct gttcgccatg ctgttcagtg ccctgtccga gtggctgttc tgggacgagt
     3721 tcggcgtgcg cttcaacttc atcgccgtcg actacctggt ctactcggaa gaagtgatcc
     3781 acaacatcct cgagtcctac ccggtattcc gcctgctcgg cgccatcgcc gtcctcgctg
     3841 gcggcctgac tctcgccctg cgtccgctgg tgcgtcgcgc cctcgacgcc gagctgccgc
     3901 cgctgcgcca gcgcctggcc ggtttcgccc tgctcgccgc aggctgcggt ctcggcttcg
     3961 ccctgctcga ccagcagcat ccgcgcggcc tcggcggcaa cgcctaccag cacgaactgg
     4021 ccagcaacgg cccgtaccag ttcttcgccg ccttccgcaa caacgagctg gactaccagc
     4081 agttctaccc gacgctggag atgaccaagg tcgccggcat cctgcgcgag gagctgcagg
     4141 agcccaatgc ccgcttcaac gacggcgagc cgctgaatat ccgccggcag atcgataatc
     4201 ctggaccggc gagcaaactg aacgtggtat tggtcaccat cgagagcctg tcggccaagt
     4261 acctcggcag cttcggcgac agccgcggcc tgacccccaa cctcgacgaa ctgcgccagc
     4321 acagcctctt cttcaacaac gtctacgcca ccggcacccg caccgaccgc ggcctggaag
     4381 ccatcaccct gtcgataccg ccgaccccgg gacgctccat cgtcaagcgc atcggccgcg
     4441 agagcggctt cgctagcctc ggccagcaac tcgcgggcca gggttacgac agcgtgttcg
     4501 tctacggcgg acgcggctac ttcgacaaca tgaacgcctt cttcagcggc aatggctacc
     4561 gcatcgtcga ccagagcagc gtgccggagc aggacatcca gttcgccaat gcctggggca
     4621 tggccgacga ggacctctat gcccaggcga tgaaggtggc cgacgccgac catgccgccg
     4681 gcaagccgtt cctgctgcaa ctgatgacca cctccaacca ccgcccctac acctacccgg
     4741 acgggcgcat cgacatcgcc tccggtgacg gtcgcgaagg ggcggtgaag tacaccgact
     4801 gggcgatcgg cgacttcctc gccaaggcgc gccagaagcc ctggttcgac gacaccgtgt
     4861 tcatcttcgt cgccgaccac tgcgccggca gcgccgggat ggaggatctg ccggtggcca
     4921 actaccacat cccgctgttc gtctatgcac cgaagtggat ccaggcccgc gaggacggcc
     4981 agctggccag ccagatcgac ctggcgccga ccctgctcgg cctgctcggg gcggactacg
     5041 aatcgacctt cttcggtcgc gacctgctgc agccgcaccg caatccgccg cgggtactga
     5101 tcggcaacta ccagcacctg ggactgttcg acggccgcaa cctggcaatc ctcagcccga
     5161 accggctgat gcgccggcat gacgacgccc tcggcgccag ccgcgaacac acggccaacc
     5221 gcgacgatgc cctgatcgat cgcgacatcg cctactacca gagcgccagc tacgccttca
     5281 gcaacggcct gctgacctgg cgcggccagg cgccggccac cacgaccttc ggcctagccg
     5341 ggcaaacgga gcgcgcaaac tgatgaacct ctcgattcag ggcgcctcgc gcccgctgcg
     5401 cacctggctg gcgctggggc tgcccctggc actgatcgtg ctgctgctgg tgttcgagcc
     5461 cacccgcctc gacttcgccc tgagcgacct gttctaccag ccgggcgtgg gcttcagcgg
     5521 cgggcacagc gcgtttctcg aagacttcct gcacgatcgc gccaagcagg gtgtgatcgt
     5581 catcggcgtg ctggccatcg ctggcctgct gtcgagctgg ctgtaccggc cctgggcggc
     5641 gctgcgccgg ccattcggct acctggtgct ggcgatggcg ctgtccaccg ccatcgtcaa
     5701 tccggtgaag accctgaccg gggtgcactg cccgtggagc ctgacccagt acggcgggga
     5761 agaaacctac agcccgctgc ttggcgagcg tgctccggtc gcggacaagg ctgggcgctg
     5821 ctggcccggt ggccacgcca gcagcggctt caccctgttc gccctgtatt tcgtcctgcg
     5881 cgaccgtaaa ccgcgcctgg cgcgcgccgc actgctgttc gccctcggcc tcggcacggt
     5941 gttctcgatg ggccgggtca tgcagggcgc gcactttctc tcgcacaacg tctggacggc
     6001 gctgttcgac tggatgatct gcctcggctt ttatcgcctg atcctctatc gccaggccca
     6061 acccgccgtg gcgccggccc tggccactcg gcggctggaa agcgccgact gagtaaccgt
     6121 gtcccgagtc aagtggtttt gatctcggga ttccaaggca gtccgctcct ggctatggca
     6181 ttgagcgtgg tcaggagctt gcgcatgcag gcaacgagcg cgaccttggg cagcttgccg
     6241 gcggccagca ggcgagcata gaacgcggcg atggccgggt tgtggcgcgt ggccgtcaac
     6301 gtggccatgt agagc
//
LOCUS       DATCUN010000231        16071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_104261, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000231 DATCUN010000000
VERSION     DATCUN010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 16071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16071
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104261"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VIO83_08815"
     CDS             <1..520
                     /locus_tag="VIO83_08815"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249173.1"
                     /note="catalyzes the formation of malate from glyoxylate
                     and acetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEY8331411.1"
                     /translation="HKVDVFARQQELAKRERASLDDILTIPLAPSISWSAEEIQNELD
                     NNAQGILGYVVRWIDQGVGCSKVPDINDIGLMEDRATLRISSQHIANWLRHGVCSVEQ
                     VMETMKRMALVVDRQNANDPLYRPMAPNFDDNIAFQAAVELCVEGTKQPNGYTEPVLH
                     RRRREFKAKNGL"
     gene            707..1255
                     /locus_tag="VIO83_08820"
     CDS             707..1255
                     /locus_tag="VIO83_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY8331412.1"
                     /translation="MRRLRFLLGLPLLLGGTAAQAANCGPLLEGNLPALRGQQTIDLC
                     RFAGKPLVVVNTASHCGFTGQFKGLEALYQRYKGQGLEMLGVPSDDFHQEADDSAETA
                     SVCYVNYGVTFSMAATQRVTGNEAIPLFRNLAAQSGAPRWNFYKYVIDRQGKVVAHFS
                     SMTRPDDAKLIAAVEQAIASQP"
     gene            1337..3271
                     /locus_tag="VIO83_08825"
     CDS             1337..3271
                     /locus_tag="VIO83_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004419484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HEY8331413.1"
                     /translation="MSKNDGFAEVGKTAVMQNIQGTMHFLLRYAPFNQMELSHLAQMV
                     EQCRLRFYAAGDPIVGPGDGRIDNVYIVKQGLVLGQRPAQNSEGMETTQEIAPGECFP
                     VAAMVSERATRSHYIAAEDTFCLQFNRAAFTHTYNQSEAFRDFALRGVSSLVGQAHQQ
                     VQLQAAETLGAQYSLETRLNILAAANPVTCRSGISLRDAVKLMHESHVGSLIMVDDSH
                     YPIGIFTLRDLRRVIADGSGDLQQPLDELMTRDPIHLPPTATAFDAALIMTRRQIAHL
                     CVVEMGRLVGVLSERDLFSLQRVDLVHLARTISAAPQIDILVELREQIAHLIDNMLAH
                     GANPEQLTHIITQLNDHTVERVIELMIEEHGDPGEPFTWLAFGSEGRREQALLTDQDN
                     GILFEADSPEEAEQIRQRLLPLARKINEALDRCGFALCNGNIMAGNPELCLSRQEWLR
                     RFSGMIQSPTRDNLMHSSIFFDLRPVWGPSEGCRQLSEALLQMIEDNALFQHLMAANT
                     LRHPPPIGRFRDFIVARLGADKDTLDLKVEGLMPFVEGIRILALAHGISEGNTLERLR
                     QLGARGVLKPQDAAAYEEAYDFIQMTRMQLHQRQERDGQPLSNRLAPDTLNQLDRRIL
                     RESFRQAQRLQNSLRVRYQL"
     gene            3268..3987
                     /locus_tag="VIO83_08830"
     CDS             3268..3987
                     /locus_tag="VIO83_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003194822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY8331414.1"
                     /translation="MNPLRALFSRLQRRHLPLDDQQRQRLRALPPPPALDAPLHQQRF
                     VVLDLETTGLNLSRDIVISIGAVTIEDGAIDMAQQFECTLRRQVKVNEAVLIHGIAPS
                     ELAAGQPPAEALLSFMEFAADSVMLAFHAPFDQRMLARALQKELGYTLKNPFLDVADL
                     APMLFPEVLTRRGSLDYWMNYFNIDIAQRHHASADALATAEIALVLFNRARRMGIERV
                     EELAQRARYWQRTRQAALNSI"
     gene            complement(4052..5893)
                     /locus_tag="VIO83_08835"
     CDS             complement(4052..5893)
                     /locus_tag="VIO83_08835"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HEY8331415.1"
                     /translation="MSDSFKMDNLPFSLLDAREQALLNSHFDIAHFHKGELIIEAGKP
                     PQGVFIISRGRVAESDVAEPDAQGKPLASNVFVHYEDEDYFGGWSAINGVAIHNFIAV
                     EATTCHVLPTSILLELMVSNSLFADYFQQNLSAKREIVAQHGQSQDMAEFMLAKISDG
                     LIREPLIVPQGTSLHEATRLMREMKTDCLLAKKRNRYGMITGTDLLDAVVLQGMPLEA
                     DVSEIASYRLITAQPDDYLFNALILMTQHQIERVVVMREDNSLAGVVELTDVLSHFSS
                     HSHVIGLRVERANTVEELREAALGLTDLIRMLISTGVKIRFTMELLAAMNGRIISKLF
                     DLIIPNDMRPHVCLIVMGSEGRGEQIMKTDQDNALLVRNGLEWPGLMDTMRRFSETLI
                     SFGFPPCPGNIMVSNPEWVNSIDQWARKLTQWSHSCDGLSVMNLAIAVDSKPIAGNIA
                     LFKVARNAFLRRVQSNDIFFSHFAKAALNFDTPLTLFGNLKDKEQLDIKKGGIFPVVH
                     GIRTMALEQRILETNTFKRIEALVAMGKMDKAMGENLGEALSIFIQLRLNQQMQRVEA
                     GGESLDKTPNAIDLQKLNKLERDLLRDALHIVKDFKKLFVMRYHISS"
     gene            complement(6199..6738)
                     /locus_tag="VIO83_08840"
     CDS             complement(6199..6738)
                     /locus_tag="VIO83_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331416.1"
                     /translation="MIWHLIAAICAALAGAGIALILRSLTRKWLPKWIIPAFAGLGML
                     AYTIHYEYTWFDTKQAQLPAGSLVVASETGDMLWRPWTALFPMPLSYTVLDSANAQIE
                     ETSKGRMGRFVLYRFEKHHLMSTVKSAAHQLLCSEKVMFSLNEAGQAKFDTMTKLDIN
                     APLYRAICEPNGLQSSLGN"
     gene            complement(6838..8607)
                     /locus_tag="VIO83_08845"
     CDS             complement(6838..8607)
                     /locus_tag="VIO83_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003301492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEY8331417.1"
                     /translation="MSQFVINMLFVGASFALYFGIAVWARAGSTKEFYVAGGGVHPVT
                     NGMATAADWMSAASFISMAGIIAAGGYSTSVYLMGWTGGYVLLAMLLAPYLRKFGKFT
                     VPDFIGDRFYSRGARLAAVVCLILISVTYVIGQMAGAGIAFSRFLEVSNSAGIWIAAA
                     VVFAYAVFGGMKGITYTQVAQYVVLIIAYTIPAVFIAFQLTGNPVPSLGMIGTHVESG
                     EPLLAKLDAVVTDLGFAAYTADTDNKLNMILFTLSLMIGTAGLPHVIIRFFTVPKVAD
                     ARWSAGWTLIFIALLYLTAPAVASMARLNLMTTVYPQGTEAAPLAYEERPEWVKTWEE
                     TGLIKWEDKNSDGRVQFYNDANATFKATATERGWNGNELTVNNDIIVLANPEIANLPG
                     WVIGLIAAGAIAAALSTAAGLLLAISSAVSHDLIKTLINPKISEKGEMKAARISMAVA
                     ILLATWLGLNPPGFAAQVVALAFGLAAATLFPALMMGIFSKRVNDKGAIAGMVVGLLV
                     TIVYIFMYLGWFFIPGTATFANTPDNWLFGISPQAFGSVGAIINFAVAYAVSLASEAP
                     PKAVQDLVESVRTPKGAGAALDH"
     gene            complement(8614..8874)
                     /locus_tag="VIO83_08850"
     CDS             complement(8614..8874)
                     /locus_tag="VIO83_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HEY8331418.1"
                     /translation="MADDKANAAAYWKANVSLITWSLVVWALVSYGFGILLRPMLMGI
                     SVGGADLGFWFAQQGSIITFIAIIFHYAWRLNKLDKEFGVEE"
     gene            9650..11050
                     /gene="dbpA"
                     /locus_tag="VIO83_08855"
     CDS             9650..11050
                     /gene="dbpA"
                     /locus_tag="VIO83_08855"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HEY8331419.1"
                     /translation="MCPYRPETVTDAFSSLPLPASLLASLDAIGYRQMTPIQAQSLPI
                     ILAGNDLIAQAKTGSGKTAAFALGLLAPLNPRYFGCQALVLCPTRELADQVAKEIRAL
                     ARGSSNIKVLTLCGGVPFGPQIGSLEHGAHIVVGTPGRILEHVRKGTLKLDGLNHLVL
                     DEADRMLDMGFVDSISEIIEQTPSKRQTLLFSATYPEGIEQLAARFMRKPQRIELESQ
                     HDDSQIEQRFYEIDPNQRMEAVARLLLHFRPASSVVFCQTKQQCQEVATMLNARKISA
                     LALHGDLEQRERDQVLAMFANQSCCVLVATDVAARGLDIDTLEAVINLELTRDPQVHI
                     HRIGRSGRAGEKGLALSLVAPSEAGRARAIEELQQAPLNWANLDNLKPLSDERLLPPM
                     TTLCINGGRKDKLRPGDILGALTGEGGLPGASIGKIALYDHNAYVAVERERAKEGLQR
                     LSERKVKGRGYKARIL"
     gene            11278..13170
                     /locus_tag="VIO83_08860"
     CDS             11278..13170
                     /locus_tag="VIO83_08860"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8331420.1"
                     /translation="MVVRPDLAHRLLILLGLVLLFALPAQPARAGLAALVLDGREQAV
                     DLHSHVSLLRDPAGSLRLEQLPARAADFVPVRQRSDLNLGYTGEVIWLRLDLQSRATE
                     PGEWRIEFSRPSLDRVELFSVGHGDLQQQLGGDSVPAAQRSFDHRAPLFAVQLAPGEQ
                     RSLYFRANSSGNLALDARLWNARAFEQYAHSASLLLALYFGLLLGLASYNLLLFVALR
                     EPSLLFYVLFVASFAAGMLGFSGLGAQYLWPQGGAWGSRALPFSLALANAAAVLLARS
                     FLDSAANAPLWHRWLRVDLGVQLAIAVGTLLLPLPLILQALVPTSIGNILLLLAYGIR
                     CMRRGVPAASVFVGAKLLLMGGAVLLALRNFGLLPSNAISVNAMQIGSALEMLLLSFG
                     LASRLNALRQQKVTTQASTLAAQQHSLQALQEQERVLEQRVAERTEALAAANERLREL
                     ALKDPLTGLANRVALRQHMEQVWQRARRRNEMLALIMLDLDGFKPVNDHYGHEAGDLL
                     LIQVARRLQASARTTDLVARLGGDEFVLVCESIGSPEQAEVLAGRILESLGRPFRLLG
                     LDVCIGASIGISFGLPPGGDCDALLREADQAMYEAKAAGRNCIRLGQPLSAEPPLGES
                     VQRDES"
     gene            13247..14431
                     /locus_tag="VIO83_08865"
     CDS             13247..14431
                     /locus_tag="VIO83_08865"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014597971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8331421.1"
                     /translation="MSSPEVVIIGAGAAGLMCALTAAARGRRVLVLDHANKAGKKILM
                     SGGGRCNFTNMYTEPANFLSHNPHFCKSALARYTQWDFIELVCKHDVPYHEKKLGQLF
                     CDRKASDILQLLLVECAEAGAEIRLDTSIEQIERTDTGYRLATTGGEFACQSLVIATG
                     GLSIPTLGATGFGYQVARQFGHSVLPTHAGLVPFTVTDPQLKALCAELSGTAVEDCLV
                     SCNGQSFKENILFTHRGLSGPAILQLSSYWQPGDTVHINLLPHIDLPEWLAEQQRERP
                     NSELKTLLAELFTKKMAGLLAEQWFVSKPLKQYTPAELNAVAECLADWTLIPAGTEGY
                     RTAEVTLGGVDTREVSSKTMESQKSPGLYFVGEVLDVTGHLGGFNFQWAWASGHAAGE
                     VV"
     gene            complement(14428..15138)
                     /locus_tag="VIO83_08870"
     CDS             complement(14428..15138)
                     /locus_tag="VIO83_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEY8331422.1"
                     /translation="MFRILREYERGVVFQLGRFWKVKGPGLILLVPGIQQMVRVDLRT
                     VVLDVPTQDVISRDNVSVKVNAVIYYRVLEPQKAIIQVEDYQVATSQLAQTTLRAVLG
                     KHELDDMLAERERLNADIQQVLDAQTDAWGIKVANVEIKHVDLDESMIRAIAKQAEAE
                     RERRAKVIHAEGELQASEKLMQAAEMLGRQDGAMQLRYMQTLGAIAGDKSSTIVFPMP
                     IDLLQSLVARDRKEEPRG"
     gene            complement(15203..>16071)
                     /locus_tag="VIO83_08875"
     CDS             complement(15203..>16071)
                     /locus_tag="VIO83_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008010358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY8331423.1"
                     /translation="RGLAQRRGRNAEWAEQAVREAASLAAEEALRLKVIDYLAADLAD
                     LLRQLDGQRFAVVDGSVTLASAGAVLHPYQPDWRTRLLAVITNPSVALILMMLGVYGL
                     FFEFSTPGFGVGGVLGGICLILALYALQLLPVSYAGVALILLGIAFMTAEAFVPSFGV
                     LGIGGVVAFVVGGLILIDTEVPGFGIPLALILTLGIGSALLIAFIAGMALKTRRRALQ
                     GGDAGLVGSLTAVVGLQAGDPQAGWVHLQGEQWQVRSRAPLQAGQRVRVIARHGLWLE
                     VAATDATSSGGD"
BASE COUNT         2974 a         5569 c         4936 g         2592 t
ORIGIN      
        1 ccacaaggtc gacgtgttcg cccgtcagca ggagctggcc aagcgcgagc gcgcctcgct
       61 ggacgacatc ctgaccatcc cgctggcgcc gagcatcagc tggagcgccg aagagatcca
      121 gaacgaactg gacaacaacg ctcagggcat cctcggctac gtggtgcgct ggatcgacca
      181 gggtgtcggc tgctccaagg tgccggatat caacgacatc ggcctgatgg aagaccgcgc
      241 caccctgcgc atctccagcc agcacatcgc caactggctg cgccacggcg tgtgctcggt
      301 cgagcaggtg atggaaacca tgaagcgcat ggcgctggtg gtcgaccgtc agaacgccaa
      361 cgacccgctg taccgcccga tggcgccgaa cttcgacgac aacatcgcct tccaggcggc
      421 cgtcgaactg tgcgtcgaag gcaccaagca gcccaacggc tacaccgagc cggtactgca
      481 tcgccgtcgc cgcgagttca aggccaagaa cggcctgtaa taccgcgtcg gcacggatgc
      541 cgactccggg agcgccctgc gctcccctcc cacaacgcgc cgcgctcctc cgggagccgg
      601 cgcgttgtgc tttgtgggcc tgccctgcca atggctgatt gccagccacg cgacgcgcgg
      661 ttagcgtgga gccatgggta cctgacagcc ccattcggag actgccatgc gtcgcttacg
      721 tttcctcctc ggcctgcccc tgctgctcgg cggcaccgct gcccaggccg ccaactgcgg
      781 cccgctgctg gaaggcaacc tgcccgccct gcgcggccag cagaccatcg acctctgccg
      841 gttcgccggc aaaccgctgg tggtggtcaa taccgccagc cactgcggct tcaccggcca
      901 gttcaagggc ctcgaggcgc tctaccagcg ctacaagggt cagggcctgg aaatgctcgg
      961 tgtgccctcc gacgacttcc atcaggaagc cgacgacagc gcggaaaccg ccagcgtctg
     1021 ctacgtcaac tacggggtca ccttcagcat ggccgccacg cagagggtca ccggcaacga
     1081 ggccatcccg ctgttccgca acctcgccgc gcagagcggc gcgccgcgct ggaacttcta
     1141 caagtacgtg atcgaccgcc agggcaaggt ggtggcgcac ttctccagca tgacccgacc
     1201 ggacgatgcg aaactgatcg cggccgtgga acaggccatc gcctcgcagc cctgaacgcg
     1261 gcgagcctgg cgcagatcag gggctgcgcc agggctcacg gcgaaccatg aacggacgcc
     1321 cacggagaac aacaagatga gcaagaacga tggcttcgcg gaggtcggca agaccgccgt
     1381 gatgcagaac atccagggca ccatgcactt cctgctgcgc tacgccccgt tcaaccagat
     1441 ggagctcagc catctggcgc agatggtcga gcagtgccgc ctgcgcttct atgccgccgg
     1501 cgatcccatc gtcggccccg gcgacgggcg catcgacaac gtctacatcg tcaagcaggg
     1561 cctggtgctg ggccagcgcc cggcccagaa cagcgagggc atggagacca cccaggagat
     1621 cgccccgggc gagtgcttcc cggtggcggc catggtcagc gagcgggcca cgcgcagcca
     1681 ctacatcgct gccgaggaca ccttctgcct gcagttcaat cgcgccgcct tcacccacac
     1741 ctacaaccag tcggaagcct tccgcgactt cgccctgcgc ggagtcagca gcctggtagg
     1801 ccaggcgcac cagcaggtgc agctgcaggc cgcggaaacc ctcggcgccc agtattcgct
     1861 ggaaacccgc ctgaacatcc tcgccgccgc caacccggtg acctgccgca gcggcatcag
     1921 cctgcgcgat gcagtgaagc tgatgcacga gagtcacgtc ggcagcctga tcatggtcga
     1981 cgacagccac tacccgatcg gcatcttcac cctgcgcgac ctgcgccggg tgatcgccga
     2041 cggcagcggc gacctgcagc aaccgctcga cgagctgatg acccgcgatc ccatccacct
     2101 gccgccgacc gccaccgcct tcgatgccgc gctgatcatg acccgccggc agatcgccca
     2161 cctctgcgtg gtggagatgg gccggctggt cggcgtgctc tccgagcgcg acctgttctc
     2221 cctgcagcgg gtcgacctgg tgcatctggc gcggaccatc agcgcagccc cgcagatcga
     2281 catcctggtc gagctgcgcg agcagatcgc ccacctgatc gacaacatgc tcgcccacgg
     2341 cgccaacccg gagcagctga cccacatcat cacccagctc aacgaccaca ccgtggagcg
     2401 ggtcatcgaa ctgatgatcg aggagcacgg cgaccccggc gagcccttca cctggctggc
     2461 gttcggcagc gaagggcgcc gcgagcaggc gctgctcacc gaccaggaca acggcatcct
     2521 attcgaggcc gacagccccg aggaagccga gcagatccgc cagcgcctgc tgccgctggc
     2581 gcgcaagatc aacgaggccc tggatcgctg cggctttgcc ctgtgcaacg gcaacatcat
     2641 ggccggcaac cccgagctgt gcctgtcccg ccaggagtgg ctgcgccgct tcagcggcat
     2701 gatccagagc ccgacccgcg acaacctgat gcactcgtcg atcttcttcg acctgcgccc
     2761 ggtctggggg ccgagcgaag gctgccggca gctcagcgag gcgttgctgc agatgatcga
     2821 agacaacgcc ctgttccagc acctgatggc ggccaacacc ctgcgccacc cgccgcccat
     2881 cggccgcttc cgcgacttca tcgtcgccag gctcggtgcc gacaaggaca ccctggatct
     2941 caaggtggag ggtctgatgc ccttcgtcga gggtatacgc atcctcgccc tggcccacgg
     3001 catcagcgag ggcaacaccc tcgagcgcct gcgccagctc ggcgcgcggg gcgtgctgaa
     3061 gccccaggat gccgccgcct acgaggaggc ctacgacttc atccagatga cccgcatgca
     3121 gctgcaccag cgccaagagc gtgacggcca gccgctgagc aaccgcctcg cccccgacac
     3181 cctcaatcaa ctggatcggc gcatcctgcg cgagtccttc cgccaggccc agcgcctgca
     3241 gaacagcctg cgcgtgcggt accagctatg aacccgctcc gcgccctgtt ctcccggctg
     3301 cagcgccgtc atctgccgct cgacgaccag cagcgccagc gcctgcgggc gctgccgccg
     3361 ccgcccgcgc tggacgcccc gctgcaccag cagcgcttcg tggtgctcga cctggaaacc
     3421 accggcctga acctgagccg tgacatcgtc atctcgatcg gcgcggtgac catcgaggac
     3481 ggtgccatcg acatggccca gcagttcgaa tgcaccctgc gccgccaggt caaggtcaac
     3541 gaggccgtgc tgatccacgg catcgccccc agcgagctgg ccgccggcca gccgccggcc
     3601 gaggccctgc tcagtttcat ggagttcgcc gccgacagcg tgatgctggc cttccacgcc
     3661 cccttcgacc agcgcatgct ggcccgcgcg ctgcagaaag aactgggcta cacgctgaaa
     3721 aacccgttcc tcgacgtcgc cgacctggcg ccgatgctgt ttccggaggt gctgacccgg
     3781 cgcggcagcc tggactactg gatgaactac ttcaacatcg acatcgccca acgccaccac
     3841 gcctcggcgg atgccctggc gaccgcggag atcgccctgg tcctgttcaa ccgcgcccgg
     3901 cgcatgggca tcgagcgggt cgaggagctg gcccagcggg ccaggtactg gcaacgcacc
     3961 cgccaggcgg cgctcaattc gatctgaacg gagcgcacat gaacctgtag gagccagctt
     4021 gctggctcct acaggaaacc cggcacgcgg ctcagctgct gatgtgataa cgcatgacaa
     4081 acagcttctt gaagtccttc acgatgtgca gggcgtcgcg taacaggtca cgctccaact
     4141 tgttcagttt ctgcagatcg atggcattgg gcgtcttgtc cagactctct ccgccggctt
     4201 cgacacgctg catctgctga ttcaggcgca gctggatgaa gatcgacaag gcctcgccaa
     4261 ggttctcgcc catggccttg tccatcttgc ccatcgccac cagcgcctcg atgcgcttga
     4321 aggtgttggt ctccagaatc cgctgctcca gcgccatggt tcttatccca tgcacgaccg
     4381 ggaaaatacc gcccttcttg atgtccagct gttccttgtc cttcaggttg ccgaagaggg
     4441 tcagaggcgt atcgaagttc agcgccgctt tcgcaaagtg cgagaagaag atgtcgttgc
     4501 tctgcacgcg gcgcaggaag gcatttctcg ccaccttgaa cagggcgatg ttgccggcaa
     4561 tcggcttgct gtccacggca atcgccaggt tcatcaccga cagaccatcg caactatgac
     4621 tccactgcgt cagcttgcgc gcccactggt cgatcgagtt cacccactcc gggttcgaga
     4681 ccatgatatt gcccggacag ggcgggaagc cgaagctgat caaggtctcg ctgaagcggc
     4741 gcatggtgtc catcagcccg ggccactcca ggccgttgcg caccagcagc gcattgtcct
     4801 gatcggtctt catgatctgc tcgccgcgcc cttcggagcc catcacgatc aggcagacgt
     4861 gcgggcgcat gtcgttgggg atgatcagat cgaacagctt ggaaatgatc cggccattca
     4921 tcgccgccag cagttccatg gtgaagcgga tcttcacccc tgtggatatc agcatcctga
     4981 tcagatcggt gagccccagc gccgcctccc gcagctcctc gaccgtattg gcgcgctcga
     5041 cccgcagacc gatgacatgg gagtggctgg agaagtggct gagcacatcg gtcagctcga
     5101 cgactcctgc caggctgttg tcttcgcgca tgaccacgac acgctcgatc tgatgctggg
     5161 tcatcaggat cagcgcattg aacagatagt cgtcaggctg cgccgtgatc agacgatagc
     5221 tggcaatttc ggagacatcc gcttccagcg gcatcccctg caacaccacc gcatcgagca
     5281 ggtccgtccc ggtgatcatg ccgtaacgat ttcttttctt cgccagcagg cagtccgtct
     5341 tcatctcgcg catgaggcgc gtcgcctcat gcagactggt cccttgcgga acgatcagag
     5401 gctcacgaat taggccatcg ctgattttgg ccagcatgaa ctcggccatg tcctggctct
     5461 gcccgtgctg cgcgacgatt tcccgcttgg cgctcagatt ctgctggaag taatcggcaa
     5521 acagtgaatt gctgaccatc aactccagca ggatgctggt cggcagcaca tggcaggtgg
     5581 ttgcctcgac cgcgatgaag ttgtgaatcg ccactccatt gatcgccgac cagccaccga
     5641 aataatcctc gtcttcgtaa tgcacgaaaa cattactggc cagcggcttg ccctgcgcat
     5701 ccggctcggc cacgtcgctt tccgcgactc ggcccctgga tatgatgaat accccctgcg
     5761 gcggcttgcc cgcctcgatg atcagctcgc ccttgtggaa gtgggcaata tcgaaatgac
     5821 tgttcagcaa ggcctgctct cgggcatcca gcagactgaa gggcaggtta tccatcttga
     5881 acgagtcaga catgacaagc gctccgcttt agttattgtt cctgtccgcc actcgcagcg
     5941 acaagtccgc ccagcctcgc catcctgcag gcaagcagat gcatgagcat gattaaagcg
     6001 acaaaggagg gaggtgtcac gcgtcaatgc cgcgcactgt ggatgccttt tccggcagtc
     6061 gcggacacgg tttcagacgc actgccgcag tcgactgacg gggatgcagc gaattctcag
     6121 actgcgcagc aggtgctccg cagcgacatt gccatgctgc tggcggtcag gcgaccgcca
     6181 ttgaatcgtg cggccggatc agttgcccag ggaggactgc agaccgttgg gctcgcagat
     6241 ggcccggtag agcggtgcat tgatatccaa cttggtcatg gtgtcgaact tggcctgccc
     6301 ggcctcgttc aggctgaaca tcaccttctc gctgcagagc aattgatggg cggcgctttt
     6361 cacggtggac atcagatgat gcttttcaaa gcggtaaagg acgaaacgac ccatgcgccc
     6421 cttgctcgtt tcctcgatct gcgcattggc ggaatcaagc accgtatagg acagcggcat
     6481 cgggaacagg gcagtccagg ggcgccagag catgtcgccg gtttcgctgg ccaccaccag
     6541 cgagcctgcc ggtagttgcg cctgtttggt atcgaaccag gtgtattcgt agtggatggt
     6601 ataggcgagc atgcccaggc cggcgaaggc cggaatgatc cacttcggca gccacttgcg
     6661 cgtcaggctg cgcagtatca aggcgattcc cgcacctgcc agggcagcgc agatggcggc
     6721 aatcagatgc catatcatat tcagtcccct caaaagaatc gggcttccaa ttggaagccc
     6781 gatatcagtt tcaaccgtgg atcagagtat tagtctgact tcgggccaaa ttatcactta
     6841 gtgatccagc gcggcgccag cacccttcgg agtacgcacg ctctccacca gatcctggac
     6901 tgccttcggc ggagcctcgg aggccagcga cacagcgtag gccactgcga agttgatgat
     6961 cgcacccacg gaaccgaagg cttgcgggga gataccgaac agccagttat ccggagtgtt
     7021 ggcgaaggtc gcggtgcccg gaatgaagaa ccagcccagg tacatgaaga tgtagacgat
     7081 ggtgaccagc aggccgacaa ccatgcctgc gatcgcgccc ttgtcgttca cgcgcttgga
     7141 gaagataccc atcatcagtg ccgggaacag ggtcgctgcc gccagaccga aggccagtgc
     7201 cactacctgt gccgcgaagc ccggaggatt caggcccagc caggtggcca gcaggattgc
     7261 cacggccatg gagatccgcg cggctttcat ctcgcccttc tcgctgatct tcggattgat
     7321 cagggtcttg atcaggtcat ggctcaccgc ggaggagatg gccagcagca gaccggcagc
     7381 ggtggacagt gcagcggcga tggctcccgc agcgatcaga ccgatgaccc agcccggcag
     7441 gttggcaatt tccgggttgg ccagcacgat gatgtcgttg ttgacggtca gctcgttgcc
     7501 gttccagcca cgctcggtag cggttgcctt gaaggtggcg ttggcatcgt tatagaactg
     7561 gacgcggcca tcgctgttct tgtcttccca cttgatcagg ccggtttctt cccaggtctt
     7621 gacccactcg ggacgctctt cataggccag aggcgcagcc tcggtaccct gcgggtacac
     7681 ggtggtcatc aggttcagac gcgccatgga ggccacggcc ggagcggtga ggtacagcag
     7741 ggcgatgaag atcagcgtcc agccagcgga ccagcgagca tcagctacct tcggtacggt
     7801 gaagaagcgg atgatgacgt gcggcaggcc agcggtaccg atcatcagcg acagggtgaa
     7861 caggatcatg ttcagcttgt tgtcggtgtc ggcggtatag gcggcgaagc ccagatcggt
     7921 gaccacggca tccagcttgg ccagcagcgg ctcgccagac tcgacgtggg taccgatcat
     7981 gcccagcgac ggaaccgggt tgccggtcag ctggaaggcg atgaacactg ccgggatggt
     8041 gtaggcgatg atcagcacca cgtactgggc cacctgggtg taggtgatgc ccttcatgcc
     8101 gccgaatacc gcgtaggcga acaccacagc agcggcgatc cagataccgg cagagttgct
     8161 cacttcgagg aagcgggaga aggcaatacc ggcaccagcc atctggccga tcacgtaggt
     8221 cacggagatc aggatcaggc agactacagc ggccaggcgc gcgccacggc tgtagaagcg
     8281 gtcaccgatg aagtccggta cggtgaactt gccgaacttg cgcaggtagg gcgccagcag
     8341 catggccagc agtacataac caccggtcca gcccatcagg tagacagaag tggaataacc
     8401 gccagccgcg atgatgccgg ccatggagat gaaggaagcc gcggacatcc agtcggccgc
     8461 agtggccata ccgttggtca ccggatgcac gccgccaccg gcaacgtaga actccttggt
     8521 cgatccggcg cgtgcccaga ctgcgatacc gaagtagaga gcgaaggaag cccctacgaa
     8581 tagcatgttg attacaaatt ggctcatgct ggcttactcc tcaacgccga attctttgtc
     8641 cagtttgttc agccgccacg cgtagtggaa gatgatcgca atgaacgtga tgatcgagcc
     8701 ctgttgagcg aaccagaagc ccaggtccgc cccaccgacc gaaataccca tcagcatcgg
     8761 gcgcaagagg atgccaaaac catatgaaac cagagcccag accaccaggc tccacgtgat
     8821 aaggctaacg ttcgccttcc agtacgcagc agcattcgct ttgtcgtctg ccatcgcctt
     8881 tctccatgtt gtttttattg caggtgaaag aaaccgcatg ctgactttag ctaggtggcc
     8941 acataaaaag aaagccccga ctttagtctg aagactgttc cgtaaattct tccggcccgc
     9001 accggacagg ccatcgctca ccgctaacga acccggcaag cgccggcagc cttggcgtgg
     9061 cgcactgcca ggccgggatg aagcgcccgc caccgacggc gcgccaggca cctgacggcc
     9121 gagcagcaag gcgctcgaag ctctcccgct gcctgcccaa ctgcccaggc cgcattccag
     9181 ccacctgcgc tgcaaccgct ggcgtcgctc cccgcaactg acgctgccca ggcaggcctc
     9241 atcacgcgca ccctgccgca ggtcgccagc tcaactgccc ggccgctgcg cttcccaacg
     9301 gcacaggccg cacctgtata taaaggaagg gcagttcggg gcgcagtact gccacaaccc
     9361 aaaaccgagc agacaacttt cattcaatac ccttgaatat tgaaaagctg cactcgacag
     9421 aacttcatga acccaaaaaa acgcccggca atatcaatta caaagtcaag acaaaaactc
     9481 ttaacaacag aaaaaaacac ccaatcaaaa tctgagcaca tcgaaaccca acaaccaaaa
     9541 ctttgccaac aacattaaca aggcaaaacc tgcacaggcc ttgacagaag cgctaccgac
     9601 aacctgcagg cggcggcagc aggactgcgg cctgttacac tgtgcgtcca tgtgcccata
     9661 cagacccgaa accgtgaccg acgccttctc ctccctgccg ctgcccgcca gcctcctcgc
     9721 cagcctggat gccatcggct accggcagat gacgccgatc caggcgcaga gcctgccgat
     9781 catcctcgcc ggcaacgacc tgatcgccca ggccaagacc ggcagcggca agaccgccgc
     9841 cttcgccctc ggcctgctgg cgccgctcaa cccgcgctac ttcggctgtc aggcgctggt
     9901 gctgtgcccg acccgtgaac tggccgacca ggtggccaag gaaatccgcg cgctggcgcg
     9961 gggctccagc aacatcaagg tgctgaccct gtgcggcggc gtgccgttcg gcccgcagat
    10021 cggctcgctg gagcatggcg cgcacatcgt cgtcggcacc ccggggcgca tcctcgagca
    10081 cgtgcgcaaa ggcaccctca aactcgacgg cctcaaccac ctggtcctcg acgaagccga
    10141 ccgcatgctc gacatgggtt tcgtcgactc gatcagcgag atcatcgagc agaccccgag
    10201 caagcggcag accctgctgt tctccgccac ctacccggaa ggcatcgagc agctagccgc
    10261 acgcttcatg cgcaagccgc agcgcatcga gctggagtcg cagcacgacg acagccagat
    10321 cgagcagcgc ttctacgaga tcgatcccaa ccagcgcatg gaagcggtgg cgcgcctgct
    10381 gctgcacttc cgcccggcct caagcgtggt gttctgccag accaagcagc agtgccagga
    10441 agtggcgacg atgctcaatg cgcgcaagat ctcggcgctg gccctgcacg gcgatctgga
    10501 gcagcgcgag cgcgaccagg tgctggcgat gttcgccaac cagagctgct gcgtgctggt
    10561 ggccaccgac gtggccgcgc gcggactgga catcgacacc ctggaagcgg tgatcaacct
    10621 cgagctgacc cgcgacccgc aggtgcacat ccaccgcatc ggccgcagcg gccgcgccgg
    10681 cgagaaaggc ctggcgctgt cgctggtggc gccgagcgaa gccggccgcg cccgcgccat
    10741 cgaggagctg caacaggcac cgctgaactg ggccaacctg gacaacctca agccgctgtc
    10801 cgacgaacgc ctgctgccgc cgatgaccac cctgtgcatc aacggcgggc gcaaggacaa
    10861 gctgcgcccc ggcgacatcc tcggcgccct caccggcgaa ggcggcctgc ccggcgcgag
    10921 catcggcaag atcgccctgt acgaccacaa cgcctacgtg gcggtcgagc gcgagcgggc
    10981 caaggagggt ctgcaacgcc tgagcgagcg caaggtcaag ggccgcggct acaaggcccg
    11041 gattctctga accgcgaaat gtagaacagt cgacagtcca tactgcgaaa cacttcacac
    11101 tttccgcaac cttcgccgcg accagcgact acagttgacg tatggcaatg tgccgccagc
    11161 ggcacattgc ctgcacctgc ccgacgcgga gacgccgggt gactgtccat ggaattcgcg
    11221 gattacctca tgcagcatgc atccagccgg cgcccttccc gggcgcaacc ggcaacggtg
    11281 gttgtgcgtc cggacctggc ccacaggctg ctgatcctgc tcggcctggt gctgctgttc
    11341 gccctgccgg cgcagccggc ccgcgccgga ctggccgcgc tggtgctgga cggccgcgag
    11401 caggccgtgg atctgcacag ccacgtcagc ctgctgcgcg accccgcggg cagcctgcgc
    11461 ctcgagcagt tgcccgcccg cgcggcggac ttcgtccccg tgcgccagcg cagcgacctg
    11521 aatctcggct ataccggcga ggtgatctgg ctgcgcctgg acttgcagtc tcgggctacc
    11581 gaacccggcg aatggcgcat cgagttcagc cggccgtcgc tggatcgcgt cgagctgttc
    11641 agcgtcggcc atggcgacct gcagcagcaa ctcggcggcg atagcgtgcc cgcggcgcaa
    11701 cgcagcttcg accatcgcgc cccgctgttc gccgtgcaac tggccccggg cgaacagcgc
    11761 agcctgtatt tccgcgccaa ttccagcggc aacctggccc tcgacgcccg gctgtggaac
    11821 gcccgcgcct tcgagcaata cgcccacagc gccagcctgc tgctggcgct gtacttcggc
    11881 ctgctgctgg ggctggccag ctacaacctg ctgctgtttg tcgccctgcg cgaacccagc
    11941 ctgctgttct acgtgctgtt cgtcgccagc ttcgccgccg gcatgctcgg cttcagcggc
    12001 ctcggcgccc agtacctgtg gccgcagggc ggtgcctggg gcagccgcgc cctgcccttc
    12061 agcctcgccc tggccaacgc cgccgccgtc ctgctggccc gcagctttct cgacagtgcc
    12121 gccaatgcgc cgctctggca tcgctggctg cgggtcgatc tcggcgtgca gctggccatc
    12181 gccgtcggca ccctgctgct gccgctgccg ctgatcctgc aggcgctggt gccgaccagc
    12241 atcggcaaca tcctgctgct gctggcctac ggcatccgct gcatgcggcg gggcgtaccg
    12301 gcggcctcgg tgttcgtcgg tgccaagctg ctgctgatgg gcggggcggt gctgctggcg
    12361 ctgcgcaact tcggcctgct gccgtcgaac gccatctcgg tgaacgccat gcagatcggc
    12421 tcggcgctgg agatgctgct gctgtccttc ggcctggcgt cgcggctcaa cgcgctgcgg
    12481 cagcagaagg tcaccaccca ggccagcacc ctcgccgccc agcagcacag cctgcaggcc
    12541 ctgcaggagc aggagcgggt actcgagcaa cgcgtggccg agcgtaccga ggcgctcgcc
    12601 gccgccaacg agcgcctgcg cgagctggcg ctgaaggacc cgctgaccgg cctggccaac
    12661 cgcgtggcgc tgcgccagca catggagcag gtctggcagc gcgcccgccg gcgcaacgag
    12721 atgctcgcgc tgatcatgct cgacctcgat ggcttcaagc cggtcaacga ccactacggc
    12781 cacgaggccg gcgacctgct gctgattcag gtcgccaggc gcctgcaggc cagcgcgcgc
    12841 accaccgacc tggtggcgcg cctgggcggc gacgagttcg tgctggtctg cgagagcatc
    12901 ggcagcccgg aacaggccga ggtgctggcc gggcgcatcc tggaaagcct cgggcgaccg
    12961 ttccgcctgc tcggcctcga cgtgtgcatc ggcgccagca tcggcatcag tttcggcctg
    13021 ccccccggcg gcgactgcga cgccctgctg cgcgaggccg accaggcgat gtacgaggcc
    13081 aaggccgccg ggcgcaactg catccgcctc ggccagccgc tcagtgcgga gccaccgctc
    13141 ggcgagtccg tccagcgcga cgagagctga accgccacgg ccctcggcct gagccccggc
    13201 gctttgctac catacgcgcc cgtcccgccc gccagagagt tccgccgtgt ccagtcccga
    13261 agttgtcatc ataggcgccg gtgccgccgg cctgatgtgc gcgctgaccg ccgccgcccg
    13321 cgggcgccgc gtgctggtgc tggaccacgc caacaaggcc ggcaagaaga tcctcatgtc
    13381 cggcggcggg cgctgcaact tcaccaacat gtacaccgag ccggccaact tcctctcgca
    13441 caatccgcac ttctgcaagt cggcgctggc ccgctacacc cagtgggact tcatcgagct
    13501 ggtgtgcaag cacgacgtgc cctaccacga gaagaagctc ggccagctgt tctgcgaccg
    13561 caaggccagc gacattctgc agctgctgct ggtcgagtgc gccgaggccg gcgccgagat
    13621 ccgcctcgac acctcgatcg aacagatcga gcgcaccgac accggctacc ggctggccac
    13681 cactggcggc gagttcgcct gccagtcgct ggtgatcgcc accggcggcc tgtcgatccc
    13741 caccctcggc gccaccggct tcggctacca ggtcgcccgc cagttcggcc acagcgtgct
    13801 gccgacccac gccggcctgg tgccgttcac cgtcaccgac ccgcagctca aggcgctgtg
    13861 cgccgagctg tccggcacag cggtggaaga ctgtttggtg agctgcaacg ggcagagctt
    13921 caaggagaac atcctgttca cccaccgcgg cctctccggc ccggcgatcc tgcagctctc
    13981 ctcctactgg caaccgggcg acacggtgca catcaatctg ctgccgcaca tcgacctgcc
    14041 cgaatggctg gccgagcagc agcgcgagcg ccccaacagc gagctgaaga ccctgctcgc
    14101 cgagctgttc accaagaaga tggccggcct gctggccgag cagtggttcg tctccaagcc
    14161 gctcaagcag tacaccccgg ccgagctcaa cgcggtggcc gagtgcctgg ccgactggac
    14221 gctgatcccg gccggcaccg agggctaccg caccgccgaa gtcaccctcg gcggcgtgga
    14281 cacccgcgag gtgtcgtcga agaccatgga gtcgcagaaa tcgcccgggc tgtacttcgt
    14341 cggcgaggtg ctggacgtca ccggccacct cggcggcttc aacttccagt gggcctgggc
    14401 ctccgggcat gccgccgggg aagtggtcta gccgcgcggc tcctccttcc ggtcgcgcgc
    14461 caccaggctc tgcagcaggt cgatgggcat cgggaagacg atggtcgagc tcttgtcgcc
    14521 ggcgatggcg ccgagggtct gcatgtagcg cagctgcatg gcgccgtcct ggcggccgag
    14581 catctcggcg gcctgcatga gcttctccga ggcctgcagc tcgccctcgg cgtggatcac
    14641 cttggcgcgc cgctcgcgct cggcctcggc ctgcttggcg atggcgcgga tcatcgactc
    14701 gtcgaggtcg acgtgcttga tctcgacgtt ggccaccttg atcccccagg cgtcggtctg
    14761 cgcatcgagc acctgctgga tgtcggcgtt gagccgctcg cgctcggcga gcatgtcgtc
    14821 cagctcgtgc ttgccgagca ccgcgcgcag ggtggtctgc gccagctggc tggtggccac
    14881 ctggtagtcc tcgacctgga tgatcgcctt ctgcggctcg agcacccggt agtagatcac
    14941 cgcgttgacc ttgaccgaga cgttgtcgcg ggagatcacg tcctgggtcg gcacgtcgag
    15001 caccacggtg cgcaggtcga cccgcaccat ctgctggatg cccggcacga ggaggatcag
    15061 ccccggcccc ttgaccttcc agaagcgccc gagctggaat accacgccgc gttcgtactc
    15121 gcgcaggatg cggaacatcg acgccagcag gccgatcagc agcgccagca gtaccccgaa
    15181 actcaactcg aaacccagca tgtcagtcgc ctcccgagga ggtggcgtcc gtcgccgcga
    15241 cctccagcca taacccatgg cgggcgatca cccgcacccg ttgtcccgcc tgcagcggcg
    15301 cgcggctgcg cacctgccat tgctcgccct gcaggtgcac ccagccggcc tgcgggtcgc
    15361 cggcctgcag ccccaccacc gcggtcaggc tgccgaccag cccggcatcg ccgccctgca
    15421 gcgcgcgccg gcgcgtcttc agggccatgc cggcgatgaa cgcgatcagc agcgcgctgc
    15481 cgatgccgag ggtcaggatc agcgccagcg gaatgccgaa gccgggcacc tcggtatcga
    15541 tcaggatcag cccgccgacc acgaaggcca ccacgccgcc gatgccgagc acgccgaagc
    15601 tgggcacgaa cgcctcggcg gtcatgaagg cgatgccgag caggatcagc gccaccccgg
    15661 catagctcac cggcagcagt tgcagggcgt acagggcgag gatcaggcag atgccgccga
    15721 gcacgccgcc gacgccgaaa ccgggggtgg agaactcgaa gaacagcccg tagacgccga
    15781 gcatcatcag gatcagcgcc acgctgggat tggtgatcac cgccagcagg cgcgtgcgcc
    15841 agtccggctg gtaggggtgc agcacggcgc cggcgctggc cagggtcacg ctgccgtcga
    15901 ccacggcgaa acgctggccg tccagctgac gcagcaggtc ggccaggtcg gcggccaggt
    15961 agtcgatcac cttgaggcgc agcgcctcct cggcggccag gctggccgcc tcgcgcaccg
    16021 cctgctcggc ccactcggca ttgcgtccgc gccgctgggc gaggccgcgg a
//
LOCUS       DATCUN010000232         8338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_66303, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000232 DATCUN010000000
VERSION     DATCUN010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8338
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66303"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1722)
                     /locus_tag="VIO83_08880"
     CDS             complement(70..1722)
                     /locus_tag="VIO83_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8331424.1"
                     /translation="MSLEQYAHTHTVTNQPTPLDGSNLYRGDRPLQEWLQRFGASWGE
                     ARLAAYGDLAGGPLLAAGFAANRHLPELHSHDRYGYRIDQVEFHPAWHRLLQAGIGHG
                     LHALPWREPRPGAQVLRAGLFYLHNQAEAGSACPLTMTHAGVAVLRRQPELAETWLPK
                     VLSLDYDPQPLPAAQKRGLTLGMALTEKQGGTDLRANSTRAFALGAMGPGQAYELLGH
                     KWFCSAPMSDAFLTLAHSEGGLSCFLLPRLRPDGSHNHIHIQRLKDKLGNRSNASAEI
                     ELRGALAWMVGEEGRGIATVLEMVALTRFDCMTGSAALMRQALSQALHHCAQREVGGR
                     VLEAQPLMQNVLADLALDSEAALALSLRLGHALERPDDEQERHFARLLTAVGKYWVCK
                     RAPAFVNEAQECLGGGGYVEESILPRLYREAPVNSIWEGSGNVQCLDVLRALDRTPEV
                     AEALFAELGDGHGEARLKAQVLRLKHALHDREDLEYRARQLCGEIAVALQASLLLQAG
                     NAAVSDAFIAARLDAPAAVYGCLPRGVEVAALLARSRLQAFD"
     gene            1837..2634
                     /locus_tag="VIO83_08885"
     CDS             1837..2634
                     /locus_tag="VIO83_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEY8331425.1"
                     /translation="MNRYPSRPVVLCLSGHDPSGGAGLQADIEALLAQGCHAAPAVTA
                     LTVQDTVNVSDFRVLDRAWVLAQANAVIADLPVAAVKLGMLGSLEMVDTVVEIMASLP
                     GVPLVCDPVLRAGGGGALGKDEVGYAIRERLLPLCRIATPNLPEARILAELPAGSADQ
                     CAAKLLQCCEHLLITGGHGDETEIHNRLYSRDGSQHDFTCARLPGSYHGSGCTLASTL
                     AGRLALGEELVSAVRSALDYTWRTLRDAEQPGHGQYVPRRLPLDFCS"
     gene            2671..3297
                     /gene="thiE"
                     /locus_tag="VIO83_08890"
     CDS             2671..3297
                     /gene="thiE"
                     /locus_tag="VIO83_08890"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY8331426.1"
                     /translation="MKTLRGLYAITDSQLLDGGRLLPYVEAALRGGAKLLQYRDKSED
                     AARRLRQAEALRELCLRHGAQLIINDDAELAARLGVGVHLGQSDGSLAVARALLGRQA
                     ILGATCHAQLELAEQARQEGASYIAFGRFFNSQTKPGAPAATPELLEQARARFRLPIV
                     AIGGVTLDTAPELIARGASMVAVIHALFAAENAAEVERRARAFSALFD"
     gene            3350..4639
                     /gene="hemL"
                     /locus_tag="VIO83_08895"
     CDS             3350..4639
                     /gene="hemL"
                     /locus_tag="VIO83_08895"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY8331427.1"
                     /translation="MSRSEILFSNAQKHIPGGVNSPVRAFRSVGGTPLFFKHAQGAYV
                     TDEDDQRYVDYVGSWGPMILGHSHPDVLDAVRRQLEHGLSYGAPTAMETEMAELICSL
                     VPSMEMVRMVSSGTEATMSAIRLARGYTGRDSIIKFEGCYHGHSDSLLVKAGSGALTQ
                     GVPSSAGVPAAFAKHTLTLAYNNLAEVAATLQEVGQEVACIIVEPVAGNMNCVPPAPG
                     FLAGLRALCDQHGVVLIFDEVMTGFRVALGGAQAHFGVTPDLSTFGKIIGGGMPVGCF
                     GGKRAIMECIAPLGPVYQAGTLSGNPLAMAAGLTTLKLISRPGFHAELADYTRRMLDG
                     LQERADAAGIPFVTTQVGGMFGLYFSAAADIVTFDDVMACDVERFKRFFHLMLDGGVY
                     LAPSAFEAGFTSIAHGETELKITLDAAERAFAELKKA"
     gene            4838..5386
                     /locus_tag="VIO83_08900"
     CDS             4838..5386
                     /locus_tag="VIO83_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8331428.1"
                     /translation="MNRAGRTLLMGCLLLTTPLLGHAGDNSLLIPADGSCVLDTRPED
                     LEQALAQCRRLAEGGDGQAQFELGEYFYDASRHPQDLPQAVHWYEQASLQGHAEAQLR
                     LGTLFWHGEGVPANAMQAYIVLKMAAVNGSDEALDSADQVAEQMNREQLEHANRVLGE
                     IFRNYLQELQASDTRSPFAPLP"
     gene            complement(5511..5843)
                     /locus_tag="VIO83_08905"
     CDS             complement(5511..5843)
                     /locus_tag="VIO83_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HEY8331429.1"
                     /translation="MSKPAPIFKVIFLNQGQVFEMYAKAIYQSDLWGFLEIEEFVFGE
                     RSQLVVDPSEEKLKAQFDGVLRSFVPMHAIVRIDEVERLGTAKISEAKGGSNVMPFPM
                     PPMPDRNG"
     gene            5974..7314
                     /gene="miaB"
                     /locus_tag="VIO83_08910"
     CDS             5974..7314
                     /gene="miaB"
                     /locus_tag="VIO83_08910"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY8331430.1"
                     /translation="MAKKLFIETHGCQMNEYDSSRMADLLGEHQALELTERPEEADVI
                     LLNTCSIREKAQDRVFSQLGRWRELKLQNPGLVIGVGGCVASQEGAAIRERAPYVDVV
                     FGPQTLHRLPEMIDAARTTQQPQVDISFPEIEKFDRLPEPRVDGPNAFVSVMEGCSKY
                     CAFCVVPYTRGEEVSRPLVDVLGEILHLAENGVREVTLLGQNVNGYRGDAGAGRIADF
                     AELLHAVAAIDGIERIRYTTSHPLEFSDAIIQAHAEIPKLVKYLHLPVQSGSDRILAA
                     MKRNHTALEYKSRIRKLKAAVPDICISSDFIVGFPGETEQDFAQTMKLIEEVGFDFSY
                     SFVYSARPGTPAADLADDTPEEVKKQRLAILQQRLNQQGFENSRRMVGSVQRILVSDY
                     SKKDPGMLQGRTENNRVVNFRSSNPRLIGQFVDVHIDEALPHSLRGTLVDAASD"
     gene            7439..>8338
                     /locus_tag="VIO83_08915"
     CDS             7439..>8338
                     /locus_tag="VIO83_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY8331431.1"
                     /translation="MNAPLEHHRFILEPFEARRFANLCGQFDEHLRLIEQRLAIEIRN
                     RGNQFELVGDAQRSQTAERLLRRLYRETHTGTELTPEMVHLFLQESGLEDIALPGQTP
                     TVTLKTRKVHITPRGANQQRYVKAILDNDINFGVGPAGTGKTYLAVACAVDALEREQV
                     RRILLVRPAVEAGEKLGFLPGDLAQKIDPYLRPLYDALYEMLGFEQVAKLIEKQVIEI
                     APLAYMRGRTLNNSFIILDESQNTTLEQMKMFLTRIGFGSTAVITGDVTQVDLPRGTK
                     SGLAHVVEVLRDVPGISFTHFKAK"
BASE COUNT         1342 a         3066 c         2679 g         1251 t
ORIGIN      
        1 gctcgcaggc gagccgccgc gacggctgcg gcgcgggctg agaagcgcat tctagccgcc
       61 gcgccggcct cagtcgaacg cctgcagccg gctgcgcgcc agcagcgccg ctacttccac
      121 cccacgcggc aggcaaccgt agaccgccgc cggggcatcc aggcgtgcgg cgatgaaggc
      181 gtcgctcacc gcggcattgc cggcctgcag caacaggctg gcctgcagcg ccacggcgat
      241 ctcgccgcac agctggcggg cgcggtattc caggtcctcg cgatcgtgca gggcatgctt
      301 gaggcgcagc acctgcgcct tcagccgcgc ctcgccatgg ccgtcgccca gttcggcgaa
      361 cagcgcctcg gccacctccg gggtcctgtc cagcgcccgc agcacgtcca ggcactgcac
      421 gttgccggag ccctcccaga tcgagttgac cggtgcctcg cggtacagcc gcggcaggat
      481 gctctcctcg acgtagccgc cgccgcccag gcattcctgc gcctcgttga cgaaggccgg
      541 cgcgcgcttg cacacccagt acttgccgac cgcggtgagc agccgggcga aatgccgttc
      601 ctgctcgtcg tccgggcgct ccagggcatg ccccaggcgc aggctcagcg ccagcgccgc
      661 ctcactgtcc agggccaggt cggccaacac gttctgcatc agcggctgcg cctccagcac
      721 ccgcccgccg acctcgcgct gcgcgcagtg gtgcagggcc tggctcagcg cctggcgcat
      781 cagcgccgcc gagccggtca tgcagtcgaa acgggtcagc gccaccatct ccagcaccgt
      841 ggcgatgcca cggccctcct cgccgaccat ccaggccagg gcgccgcgca gttcgatttc
      901 cgccgaggcg ttgctgcggt tgcccagctt gtccttcagg cgctggatat ggatgtggtt
      961 gtggctgccg tccggacgca ggcgcggcag caggaagcag ctcagcccgc cctcgctgtg
     1021 cgccagggtc aggaaggcat cgctcatcgg cgccgaacag aaccacttgt ggccgagcag
     1081 ctcgtaggcc tgccccgggc ccatggcacc cagggcgaag gcgcgggtgc tgttggcgcg
     1141 caggtcggtg ccgccctgct tctcggtcag cgccatgccc agggtcaggc cgcgcttctg
     1201 cgccgccggc agcggctgcg ggtcgtagtc gaggctgagc accttcggca gccaggtctc
     1261 ggccagttcc ggctggcgcc gcagcacggc gacgccggca tgagtcatgg tcagcgggca
     1321 ggcactgccg gcctcggcct ggttgtgcag gtagaacagt ccggcgcgca gcacctgggc
     1381 accgggccgc ggctcgcgcc agggcagcgc gtgcaggccg tggccgatgc cggcctgcag
     1441 caggcggtgc caggccgggt ggaactcgac ctggtcgatg cgatagccat agcggtcgtg
     1501 gctgtgcagc tcgggcaaat ggcgattggc ggcgaagccg gccgccagca gcggaccgcc
     1561 ggccagatcg ccgtaggccg ccaggcgcgc ctcaccccag ctggcaccga agcgctgcag
     1621 ccactcctgc agcggccggt cgccgcggta caggttgctg ccatccagcg gcgtcggctg
     1681 gttggtcacg gtatgggtgt gtgcgtattg ctccagactc atcgtccacc tcctgcaggg
     1741 tgcgtctcct gcagtgaagc agaaccacgc cgcggtgcct gccgggcgcc gcgccgggca
     1801 ggccgccgcg cgactttggt ggtaagctcg gcagccatga acagataccc ttcgcgtccc
     1861 gtcgtcctct gtctttccgg ccacgacccc agtggcggtg ccggcctgca ggcggacatc
     1921 gaggccctgc tcgcccaggg ctgccacgcc gcgccggcgg tcaccgcgct gaccgtgcag
     1981 gacaccgtca acgtctccga cttccgcgtg ctcgaccgcg cctgggtgct cgcccaggcc
     2041 aacgcggtga tcgccgacct gccggtcgcc gcggtcaagc tgggcatgct cggctcgctg
     2101 gaaatggtcg ataccgtggt cgagatcatg gccagtctgc ccggcgtgcc gctggtctgc
     2161 gatccggtgc tgcgcgccgg cggcggcggc gcgctgggca aggacgaggt cggctacgcc
     2221 atccgcgagc gcctgctgcc gctgtgccgc atcgccacgc cgaacctgcc ggaagcacgc
     2281 atcctcgccg aactgcccgc gggcagcgcc gaccagtgcg ccgcaaagct gctgcaatgc
     2341 tgcgagcacc tgctgatcac cggcggccac ggcgatgaga ccgagatcca caaccgcctg
     2401 tacagccgcg acggcagcca gcacgacttc acctgtgcac gcctgccggg cagctaccac
     2461 ggttccggct gcaccctggc cagcaccctg gccggccgcc tggcgctcgg cgaggaactg
     2521 gtcagtgcgg tgcgttcggc actcgactac acctggcgca ccctgcgcga tgccgagcag
     2581 cccggtcacg gccagtacgt gccgcgccgc ctgccgctgg atttctgctc ctgacccttg
     2641 tgccgtgacc ctctgcctgg aatagcgccg atgaaaaccc tgcgcggact ctacgccatc
     2701 accgacagtc agctgctgga cgggggccgt ctgctgccct acgtcgaagc ggcgctgcgc
     2761 gggggggcga aactgctgca ataccgcgac aagtccgagg atgccgcgcg ccgcctgcgc
     2821 caggccgagg ccctgcgcga gctgtgcctg cgccacggcg cgcagctgat catcaacgac
     2881 gatgccgagc tggccgcgcg cctgggcgtc ggcgtgcacc tggggcagag cgacggctcg
     2941 ctggcggtgg cccgcgccct gctcggccgc caggcgatcc tcggcgccac ctgccacgcg
     3001 caactggaac tggccgaaca ggcccgccag gaaggcgcca gctacatcgc cttcgggcgc
     3061 ttcttcaact cgcagaccaa gcccggcgcc ccggcggcca ctcccgagct gctggagcag
     3121 gcccgtgcgc gctttcgcct gccgatcgtc gccatcggtg gcgtgaccct ggacaccgcc
     3181 cccgagctga tcgcccgcgg cgccagcatg gtggcggtga tccacgccct gttcgctgcc
     3241 gagaatgccg ccgaagtcga gcgccgcgcc cgcgccttca gcgccctgtt cgactgactt
     3301 tcctgcaggg gcgcatgcac tcgcccccat cctgactcga gaggcttcca tgtcccgctc
     3361 cgaaatcctg ttcagcaacg cccagaagca catccccggc ggcgtcaact cgccggtgcg
     3421 cgccttccgc agcgttggcg gcacgccgct gttcttcaag cacgcacagg gcgcctacgt
     3481 caccgacgag gatgaccagc gctacgtcga ctacgtcggc tcctggggcc cgatgatcct
     3541 cggccacagc cacccggacg tgctggacgc ggttcgccgg cagctggagc acggcctgtc
     3601 ctacggcgcg ccgaccgcca tggaaaccga gatggccgag ctgatctgct ccctggtgcc
     3661 gtcgatggag atggtgcgca tggtcagctc cggcaccgag gcgaccatga gcgcgatccg
     3721 cctggcgcgc ggctacaccg gtcgcgacag catcatcaag ttcgagggct gctaccacgg
     3781 ccactccgac agcctgctgg tcaaggccgg ctccggcgcg ctgacccagg gcgtgcccag
     3841 ctccgccggg gtgccggcgg ccttcgccaa gcacaccctg accctggcct acaacaacct
     3901 cgccgaggtc gccgccacgc tgcaggaagt cggccaggaa gtggcgtgca tcatcgtcga
     3961 gccggtcgcc ggcaacatga actgcgtgcc gccggcgccg ggcttcctcg caggcctgcg
     4021 cgcgctgtgc gaccagcacg gcgtggtgct gatcttcgac gaggtgatga ccggtttccg
     4081 cgtcgccctc ggcggcgctc aggcgcactt cggcgtaacc ccggacctgt ccaccttcgg
     4141 caagatcatt ggcggcggca tgccggtcgg ctgcttcggc ggcaagcggg cgatcatgga
     4201 gtgcatcgcc ccgctcggcc cggtctacca ggccggcacc ctgtccggca acccgctggc
     4261 gatggccgcc ggcctgacca ccctcaagct gatcagccgc ccaggcttcc acgccgaact
     4321 ggccgactac acccgccgca tgctcgacgg cctgcaggag cgcgccgatg ccgccggcat
     4381 cccgttcgtc accacccagg tcggcggcat gttcggcctg tacttcagcg cggccgccga
     4441 catcgtcacc ttcgacgacg tgatggcctg cgacgtggaa cgcttcaagc gcttcttcca
     4501 cctgatgctg gacggcggcg tgtacctggc gccgagcgcc ttcgaggccg gcttcacctc
     4561 catcgcccat ggcgagaccg agctgaagat caccctcgac gccgccgaac gcgccttcgc
     4621 cgagctgaaa aaggcctgaa gcggcgctgc ggcggcccac ataatgggcc gccgcagggc
     4681 aaacccggct attcttgggc tctgcaggca catcgccagc aggaaaagcc gttctgggcc
     4741 tgtcatgacc cgccggctta tttcataatg tgactgctgg cgcattgcgc cggccgggca
     4801 tcctggcccg ttgacccagg ggagatgtcc gaccgccatg aaccgtgctg gccgcaccct
     4861 gctgatgggc tgcctgttgc tgaccacccc gctgctgggc catgcagggg ataactccct
     4921 gctgattccc gccgacggca gttgcgtgct cgacactcgc ccggaagatc tcgaacaggc
     4981 cctggcgcaa tgccgccgcc tcgccgaggg tggcgacggc caggcgcaat tcgagctggg
     5041 cgaatatttc tacgatgcca gccgtcaccc gcaggatctg ccgcaggccg tgcactggta
     5101 cgagcaggcg tccctgcagg gccatgccga agcgcaactg cgcctgggca cgctgttctg
     5161 gcacggcgaa ggcgtgccgg cgaacgccat gcaggcctac atcgtgctga agatggcggc
     5221 ggtcaatggt tcggacgagg cgctggacag cgccgaccag gtggccgagc agatgaaccg
     5281 cgagcagctg gaacacgcca accgcgtgct cggcgaaatc ttccgcaact acctgcagga
     5341 gctgcaggcc agcgacaccc gctcaccgtt cgcgccgctg ccctgaagtc ttgcgtaggg
     5401 cgattgtcca ccgccagacc ccgctgcggg ggtggtagaa aatcgctacg cgagttttct
     5461 accctacgcc ctatcccgac cactcggatc gccttggcgg actgaagcct tcagccgttg
     5521 cgatcaggca tcggcggcat ggggaacggc atgacattgc tgccgccctt cgcctcgctg
     5581 atcttcgccg tgcccaggcg ctccacctcg tcgatgcgca cgatggcgtg catcggcacg
     5641 aagctgcgca gcacgccgtc gaactgcgcc ttgagcttct cctcgctggg atcgaccacc
     5701 aactggctgc gctcgccgaa gacgaactcc tcgatctcca gaaagcccca caggtcgctc
     5761 tggtagatgg ccttggcata catctcgaat acctgaccct ggttgaggaa aatcaccttg
     5821 aagataggcg cgggcttgct catggatcgc tggaccaacg gggacttgcg ggaagggcgc
     5881 gcatcctagc acatcctccc ggcggccaag cctaggaagg ctgcgcccca ctccctataa
     5941 tgcgcggctc cctgattttc ccgacgagca cccatggcca agaagctttt catcgaaacc
     6001 cacggctgcc agatgaacga gtacgacagc tcgcgcatgg ccgacctgct gggcgaacat
     6061 caagccctgg agctgaccga gcgccccgaa gaggccgacg tcatcctgct caacacctgc
     6121 tcgatccgcg agaaggccca ggaccgggtg ttctcccaac tcggccgctg gcgcgagctg
     6181 aagctgcaga acccggggct ggtgatcggc gtcggcggct gcgtggccag ccaggaaggc
     6241 gcggcgatcc gcgagcgcgc gccctacgtc gacgtggtct tcggcccgca gaccctgcac
     6301 cgtctgccgg agatgatcga cgccgcgcgc accacccagc agccgcaggt ggacatctcc
     6361 ttcccggaga tcgagaaatt cgaccgcctg cccgagccgc gcgtcgacgg ccccaacgcc
     6421 ttcgtctcgg tgatggaagg ctgcagcaag tactgcgcct tctgcgtggt gccctacacc
     6481 cgcggcgagg aagtcagccg gccgctggtc gacgtgctcg gcgagatcct ccacctggcc
     6541 gagaacggcg tgcgcgaggt caccctgctc ggccagaacg tcaacggcta ccgcggcgac
     6601 gccggtgccg ggcgcatcgc cgacttcgcc gaactgctgc acgcggtggc ggccatcgac
     6661 ggcatcgagc gcatccgcta caccaccagc catccgctgg agttctccga cgccatcatc
     6721 caggcccacg ccgagattcc caagctggtg aagtacctgc acctgccggt gcagtcgggc
     6781 tccgaccgca tcctcgcggc gatgaagcgc aaccacaccg cgctggaata caagtcgcgc
     6841 atccgcaagc tcaaggcggc ggtgccggac atctgcatca gttcggactt catcgtcggc
     6901 ttccccggcg aaaccgagca ggacttcgcg cagaccatga agctgatcga ggaggtcggc
     6961 ttcgacttct cctactcctt cgtctacagc gcccgccccg gcacccccgc ggccgacctg
     7021 gccgacgaca ccccggagga ggtgaagaag cagcgcctgg ccatcctcca gcagcgcctc
     7081 aaccagcagg gcttcgagaa cagccggcgc atggtcggca gcgtgcagcg catcctggtc
     7141 agcgactact cgaagaagga cccgggcatg ctgcagggtc gcaccgagaa caaccgggtg
     7201 gtcaacttcc gctccagcaa cccgcgcctg atcggccagt tcgtcgacgt gcacatcgac
     7261 gaggcgttgc cgcactcgct gcgcggcacc ctggtcgacg ccgccagcga ctgagccccc
     7321 gcgcggcgcc catcaccggc gccgcgcgac ttatcctcca taggcaggct ttccccctcg
     7381 ggcccctgcg gttattcttc gtccattccc ccagccgaac ggcggccaac agaagacctt
     7441 gaacgcaccc ctggaacacc atcgctttat cctggaaccc ttcgaagcac gccgcttcgc
     7501 caacctctgc ggacagttcg acgagcacct gcgcctgatc gaacagcgcc tggccatcga
     7561 gatccgcaac cgcggtaacc agttcgagct ggtcggcgac gcccagcgca gccagaccgc
     7621 cgagcgcctg ctgcgccgcc tgtaccgcga aacccacacc ggcaccgagc tgacgcccga
     7681 aatggtccat ctgttcctgc aggaatcggg gctcgaggac atcgccctgc ccgggcagac
     7741 gccgacggtg acgctgaaga cgcgcaaggt gcacatcacc ccgcgcggcg ccaaccagca
     7801 gcgctacgtc aaggcgatcc tcgacaacga catcaacttc ggcgtcggcc cggccggcac
     7861 cggcaagacc tacctggcgg tggcctgcgc ggtggacgcg ctggagcgcg agcaggtgcg
     7921 gcgcatcctg ctggtccgcc cggcggtgga agccggcgag aagctcggct tcctgcccgg
     7981 cgacctggcg cagaagatcg acccctacct gcgcccgctg tacgacgccc tctacgagat
     8041 gctcggcttc gagcaggtgg ccaagctgat cgagaagcag gtgatcgaga tagccccgct
     8101 ggcctacatg cgtgggcgca ccctgaacaa cagcttcatc atcctcgacg aaagccagaa
     8161 caccaccctc gagcagatga agatgttcct cacccgcatc ggcttcggct ccaccgcggt
     8221 gatcaccggc gacgtcaccc aggtcgacct gccgcgcggc accaagtcgg gcctcgccca
     8281 cgtggtcgag gtgctgcgcg acgtgccggg gatcagcttc acccacttca aggccaag
//
LOCUS       DATCUN010000233         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_66480, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000233 DATCUN010000000
VERSION     DATCUN010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66480"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="VIO83_08920"
     CDS             complement(<1..1583)
                     /locus_tag="VIO83_08920"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEY8331432.1"
                     /translation="MSHTPPPDRPAPPALAGRTIQPRFGQIEQSCRQLLLNHLDQHLG
                     CTFEQADDALFSLANKAANNLEQALFFDSMREVRTQRTHIIRHFHQRLAGEFAAYIEG
                     RPLHGNAPTPAARNQLSLIDHEEHEENLLLGNMVKRCQSRCAPQLDALSQRLAQLCRD
                     PATALQQKPPCAPEAIASAFRLALPDAALPLRIRRVLYELFEQQVMNSVAQLYSALNQ
                     RLIDANILPQLSDKPPRLRAPARPPTNPPRPEAQGRRSHGDSWPNEPRSEAEQLFHGV
                     TRLLSRRHGNGNGNGNDASASPGDSSRFAASAAPPAPAGTYSETELMAALTRLQQVSA
                     RDIVDKLQQPQDAKQLKARLHAELEAACTLPDQQMLTADDADIIDLVGMLFAFILDDP
                     ALPDRCKTVLSHLHTPYLKLALRDRRLFTHGDHPARQLLDCMAKAGARFAEDSDTSGL
                     QTKMQLIVERIIQDFSGDSALFPSLLQEFEAYLRRLQQRIELRERRTLDAARGHDRLQ
                     AARQQAGEQIRRVLIGRTLP"
BASE COUNT          273 a          494 c          644 g          340 t
ORIGIN      
        1 ggcagcgtcc ggccgatcag tacccgacgg atctgctcgc cggcctgctg tcgggcggcc
       61 tgcaagcggt cgtggccgcg cgcggcatcc agggtgcgcc gctcgcgtag ctctatgcgc
      121 tgctgcaggc gacgcaggta cgcctcgaac tcctgcagca ggctggggaa cagggcggag
      181 tcgccgctga agtcctgaat gatgcgctcg acgatcagct gcatcttggt ctgcagaccg
      241 gaggtatcgc tgtcttccgc aaagcgcgcg ccggccttgg ccatgcagtc cagcagttgg
      301 cgggcaggat gatcgccgtg ggtgaacagt cggcgatcgc gcagggcgag cttcaggtag
      361 ggcgtgtgca ggtgcgaaag aacggtcttg caacggtccg gcagggccgg gtcgtcgagg
      421 atgaaggcga acagcatgcc gactaggtcg atgatgtcgg catcgtcggc tgtcagcatc
      481 tgctggtcgg gcagggtgca ggcggcctcc agttcggcat gcagtcgcgc cttgagctgc
      541 ttggcatctt gtggctgctg cagtttgtcg acgatgtcgc gtgcgctgac ctgctgcagg
      601 cgggtcagtg cggccatcag ctcggtttcg ctgtaggtgc ccgccggagc gggtggcgcc
      661 gcgcttgccg caaacctgga cgagtcacct ggactcgcac tggcatcgtt gccgttgccg
      721 ttgccgttgc cgtggcgccg gcttagcagg cgtgtgacgc cgtggaacag ttgttccgcc
      781 tcgctgcggg gctcgttcgg ccagctatcg ccatggcttc tgcgcccttg tgcctcgggc
      841 cgtggtgggt tggtaggcgg ccgtgccggc gcgcgtagac ggggcggctt gtcgctgagc
      901 tgcgggagga tgttcgcatc aatcaggcgc tggttgaggg cgctgtacag ttgcgcgacc
      961 gaattcatga cctgttgctc gaacagctcg tacaggacgc ggcggatgcg caggggcagt
     1021 gctgcgtcgg gcagcgctag gcgaaaggcg ctggcgatgg cttccggagc gcaggggggc
     1081 ttctgctgca gggcggtcgc cgggtcgcgg cacagctgtg ccaggcgttg gctcagcgcg
     1141 tccagttggg gcgcacagcg gctctggcag cgcttgacca tgttgcccag cagcagattc
     1201 tcctcatgct cctcgtggtc gatgagcgac agctggtttc gggcggcggg cgtcggcgcg
     1261 ttgccgtgca gggggcggcc ttcaatataa gctgcgaact cgcccgcgag ccgttggtgg
     1321 aagtgccgaa tgatgtgcgt ccgctgcgtg cgaacctcgc gcatgctgtc gaagaacagt
     1381 gcctgctcca ggttgttggc cgccttgttg gccagggaga acagggcatc atcggcctgt
     1441 tcgaaggtgc agcccaggtg ctggtcaaga tggttcagca gcaattgacg gcagctttgc
     1501 tcgatctggc caaagcgcgg ctggatggtg cgcccggcaa gagcaggagg agcgggacga
     1561 tcgggtggtg gcgtatgact catgatggtc tggcgggcca gagctctgtg ctctcagaat
     1621 gagcgcacat ataacaagcg tggggtgatc ctgccaagtg ccagaaaaaa ccttttattc
     1681 agggttgaca atgctttgga agtccgtaga atgccgcgcc actgatacgg gaaatcggca
     1741 acgccgaaac c
//
LOCUS       DATCUN010000234        14640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_198308, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000234 DATCUN010000000
VERSION     DATCUN010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 14640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14640
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198308"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /gene="pstB"
                     /locus_tag="VIO83_08925"
     CDS             <1..698
                     /gene="pstB"
                     /locus_tag="VIO83_08925"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY8331433.1"
                     /translation="FDVQLNIPRNRVTAFIGPSGCGKSTLLRTFNRMNDLVDGCRVDG
                     EIRLDGKDIYARGVEVAELRRRVGMVFQKPNPFPKSIYENVVYGLRLQGINQKRVLDE
                     TVEWALKSAALWDEVKDRLHDSALGLSGGQQQRLVIARTVAVQPEVLLLDEPCSALDP
                     ISTLKVEELINELKGQYTIVIVTHNMQQAARVSDYTAFMYMGKLIEFGDTDTLFTNPA
                     KKQTEDYITGRYG"
     gene            781..1509
                     /gene="phoU"
                     /locus_tag="VIO83_08930"
     CDS             781..1509
                     /gene="phoU"
                     /locus_tag="VIO83_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003303228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY8331434.1"
                     /translation="MINKDGLTHHISAQFNAELEEIRSHLLAMGGLVEKQVNDAVSAL
                     VTADSGLAAQVRAVDGQINQMERDIDEECVRILARRQPAASDLRLIISISKSVIDLER
                     IGDEATKIAKRAIELCESGEAPKGYVEIRHIGEQVRKMVQEALDAFARYDADLALSVA
                     QYDKQVDREYKSALRELVTYMMEDPRSISRVLSVIWALRSLERIGDHARNIAELVIYL
                     VRGTDVRHTGLAKQGPAKADDSQA"
     gene            1699..2634
                     /locus_tag="VIO83_08935"
     CDS             1699..2634
                     /locus_tag="VIO83_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8331435.1"
                     /translation="MLNRPILLTLLSFLGLALAAPASAQTVYKHVDANGQVSYSDQAR
                     PGAQVFQFAKPGAASRLPRLREELARQVHLDTLKHAGGDTLRVRNDLFAPVEIELRLE
                     DVANASGAPERPLRWVLPPRSQIRLLTLAPRDPAQPLRYTPKLSHALGDPRLLPKPHR
                     YPLPWSGGPFRQSQGANGQYSHFTAKGRYAVDIAMPEGTPIRAAREGVVVSTYNDQAG
                     AKPEPGGNHVRILHDDGTMGVYLHLQQGSVQVASGQRVRAGTPIARSGNTGRSSGPHL
                     HFVVQRNVGLAVESIPFRFAQPVNSRPELAVGGED"
     gene            complement(2742..4079)
                     /locus_tag="VIO83_08940"
     CDS             complement(2742..4079)
                     /locus_tag="VIO83_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY8331436.1"
                     /translation="MDPSPSATLFSYFADFGLILFALLLVLLNGFFVAAEFAMVKLRS
                     TRVETIAAEHGWRGHILRTVHGQLDAYLSACQLGITLASLGLGWVGEPAFAHLLEPLL
                     GQLGIDSPALVHGISFFSAFFAISYLHIVVGELAPKSWAIRQPELLSLWTAVPLYLFY
                     WAMYPAIWVLNTSANAILRIAGQGEPGPHHEHHYSSDELKLILHSSRARDPSDQGMRV
                     LASAMELGELEVVDWANSREDLVCLDVDASLDEILDEIRRHKYSRYPVLDAEQQFIGV
                     LHIKDLLLALAGGERPAEAFDLRPLLRPLERVSRHMPLGRLLEQFRQGGAHFALVEEA
                     DAKVVGFLTMEDVLEVLVGDIQDEHRKTERGLLAYQPGKLLVRGDTPLFKIERLLEVD
                     LDEVEADTLAGLVYDTLKRLPTEDETLETHGLRIIVKKMKGPKILLAKVLKLH"
     gene            complement(4225..5520)
                     /gene="phoR"
                     /locus_tag="VIO83_08945"
     CDS             complement(4225..5520)
                     /gene="phoR"
                     /locus_tag="VIO83_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEY8331437.1"
                     /translation="MNHDWRGALTRRLLLLLGGCLLLGLITGQYAWALAFGLAGYLFW
                     CLRQLLRLHDWLKRASDELPPESSGLWGEVYDLIYQLQKRNQHARGRLQAVVDRVQES
                     TAALQDAVIMLDRQGNLEWWNRAAGTLLGLKRKQDGGQPITNLVRHPHFKKYFAQDNH
                     REPLELPSPVNDHVRLQFQLTLYGNGEHLMLVRDVTRVYQLEQMRKDFVANVSHELRT
                     PLTVITGYLETLLDNVDEVNPRWRRALQQMQQQGSRMQNLLNDLLLLARLEAAPYPSD
                     SRPVAVDLLLLSIRTDAQALSADRHRISLEADPAVKLKGSESELRSAFSNLVFNAVKY
                     SPEGGEIRIRWWADESGGHLAVQDSGIGIEAKHIPRLTERFYRVDASRSINTGGTGLG
                     LAIVKHVLIHHGGQLKIDSTMGKGSTFTCHFPPAQVVRN"
     gene            complement(5583..6272)
                     /gene="phoB"
                     /locus_tag="VIO83_08950"
     CDS             complement(5583..6272)
                     /gene="phoB"
                     /locus_tag="VIO83_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003176964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY8331438.1"
                     /translation="MVGKSILIVDDEAPIREMIAVALEMAGYECLEAENSQQAHALIV
                     DRKPDLILLDWMLPGTTGIELARRLKRDELTADTPIIMLTAKGEEDNKIQGLEVGADD
                     YITKPFSPRELVARLKAVLRRAGASDAETPIEIGGLILDPVSHRVTIDGKAAEMGPTE
                     YRLLQFFMTHQERAYTRSQLLDQVWGGNVYVEERTVDVHIRRLRKALGETCENLVQTV
                     RGTGYRFSTKG"
     gene            6536..6937
                     /locus_tag="VIO83_08955"
     CDS             6536..6937
                     /locus_tag="VIO83_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331439.1"
                     /translation="MLRISPTLLAVVGAATLALLGQVQAAEPAAVQDGMLVDAKGMTL
                     YTFDKDAMGKSSCNDSCATNWPPLAASADADEGDDWTVIQRADGSWQWAYYGRPLYTF
                     ARDQKPGDMLGDGKMNVWHVARPEAEDAGDQ"
     gene            complement(6981..7871)
                     /gene="ubiA"
                     /locus_tag="VIO83_08960"
     CDS             complement(6981..7871)
                     /gene="ubiA"
                     /locus_tag="VIO83_08960"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEY8331440.1"
                     /translation="MSLSLLKPLARLHPRAQDFIDLTRLNRPIGIYLLLWPTLTALWI
                     AAGGVPSLANLLIFTLGVILTRSAGCAINDFADRRVDGHVERTRARPLASGRMTAREA
                     LRVFAVLMAVAFGLVLLTNAITVWLSCGALALAALYPFMKRYTYYPQVVLGAAYSWGI
                     LMTFTAETGSLPAAAGLLYLANLLWTVAYDTYYAITDRDDDLRIGVKSTAILFGDADR
                     PIIAILQGLSLLCLLLAAGRFGLGLYFHLGLAAAAACFAWEYWSTRTRDGHACFRAFL
                     HNHWAGLAILAGTIADYALR"
     gene            complement(7966..8517)
                     /locus_tag="VIO83_08965"
     CDS             complement(7966..8517)
                     /locus_tag="VIO83_08965"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEY8331441.1"
                     /translation="MSQTACASPEWLATDHLHPTPNARELDWLNEQGSLTRRLTALSG
                     DTFAVEPLVEGWQILRADECAALGVPPGSEGWVREVYLRGQGLPWVFARSVAARSRLE
                     LHPFALDQLGTTSLGHLLFRDPAFTRQPIEACRYPAALLPAAVRTADLWGRRSLFCQG
                     ALGVLVAEVFLPALWQADATPHT"
     gene            8822..9094
                     /locus_tag="VIO83_08970"
     CDS             8822..9094
                     /locus_tag="VIO83_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY8331442.1"
                     /translation="MRKPELAAAVAAKADLSKEQASRVLNAVLEEITSALNREDSVTL
                     VGFGTFLQRHRGARTGKNPQTGEPVQIRASNTVSFKPGKALKDAVN"
     gene            9226..9606
                     /locus_tag="VIO83_08975"
     CDS             9226..9606
                     /locus_tag="VIO83_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017708791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEY8331443.1"
                     /translation="MKFRFLLWLLGRLLAGASRTNAEFREQLVDRDLVFQLQTQDGKI
                     ARHFVVKDQRIRSATGSVAHPAFSIAFRDSAYAFATLTAANKQLAFMQGIQNRDIQIQ
                     GNPALVIWFQGLTQLLGPRQKQAA"
     gene            9762..11075
                     /locus_tag="VIO83_08980"
     CDS             9762..11075
                     /locus_tag="VIO83_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY8331444.1"
                     /translation="MEILILIGLIVLNGLFAMSEIALVTARKARLMKLAADGDNSASV
                     ALKLGEDPTKFLSTIQIGITSIGILNGIVGEAVLAGPLSQWLQSFGIAENAANIGATA
                     AVVIVVTYVSIVVGELVPKRIGQLNPEPIARLVARPMQVLALITRPFVMLLTVSTHAL
                     LRLLGVKQSSQASVTEEEIHAMLEEGSEAGVIEQHQHEMVRNVFRLDDRQLGSLMIPR
                     SDIVCVDIRRSPEENLQVLIESEHSRFPVCDGSLDKLLGVIHAKQALARVAKGETPDY
                     SANLQPCVYVPETLTGTELLEQFRANDMQMAFVIDEYGEIEGIVTLQNVLEAVTGEFT
                     PRNAEDAWAVQREDGSWLLDGAIPIPEMKDRLELKNLPEEDKGRYHTISGMIMLLLGR
                     VPGTGNHVDWAGWRFEVVDMDDKRIDKVLATPLLNGDESEASAQE"
     gene            complement(11225..13303)
                     /gene="recG"
                     /locus_tag="VIO83_08985"
     CDS             complement(11225..13303)
                     /gene="recG"
                     /locus_tag="VIO83_08985"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY8331445.1"
                     /translation="MTELARIAVTTLKGVGPALAEKLAKVGLESLQDVLFHLPLRYQD
                     RTRVSPIGALRPGGDAVVEGVVSGADIVLGRRRSLLVRLQDASGTLSLRFFHFSQAQK
                     DALKRGTCLRCYGEVRTGATGLEIYHPEYRALAADEPSAAVEQSLTPIYPSTEGLTQQ
                     RLRALSQQALGSLNGHSLPDWLPAELARQYDLAPLADAIRYLHRPPPDADLEELAEGR
                     HWAQHRLAFEELLTHQLSLQRLRESIRAQQAPPLAKAKRLPAQFLANLGFQPTGAQQR
                     VAGEIAWDLEQHEPMLRLVQGDVGAGKTVVAALAALQAIEAGYQVALMAPTEILAEQH
                     FLNFGKWLEPLGIEVAWLAGKLKGKARASALERIAAGAPMVIGTHALFQDEVKFQRLA
                     LAIIDEQHRFGVQQRLALRQKGVDGRLCPHQLIMTATPIPRTLAMSAYADLDTSILDE
                     LPPGRTPVTTLVIADSRRPEVIERVRAACAEGRQAYWVCTLIEESEELTCQAAQTTFE
                     ELSAALGELRVGLIHGRMKPAEKAAVMEEFKAARLQLLVATTVIEVGVDVPNASLMII
                     ENPERLGLSQLHQLRGRVGRGSAVSHCVLLYHAPLSHMGRERLAIMRETSDGFVIAEK
                     DLELRGPGEMLGTRQTGLLQFKVADLMRDADLLPAVREAAQTLLAHWPQHAAPLLERW
                     LRHGQQYGQV"
     gene            complement(13300..14238)
                     /locus_tag="VIO83_08990"
     CDS             complement(13300..14238)
                     /locus_tag="VIO83_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HEY8331446.1"
                     /translation="MTLTELRYIVTLAQEQHFGRAAERCHVSQPTLSVGVKKLEDELG
                     VLIFERSKSAVRLTPVGEGIVAQAQKVLEQAQGIRELAQAGKNQLAAPLKVGAIYTVG
                     PYLFPHLIPQLHRVAPQMPLYIEENFTHVLRDKLRTGELDAIIIALPFAEADVLTKPL
                     YDEPFFVVMPADHPWTARQTIDSAMLNDKSLLLLGEGHCFRDQVLEACPTTRKGSEGQ
                     QHTTVESSSLETIRHMVASGLGISVLPFTAVDSHHYAPGVIAIRPLTPPVPYRTVAIA
                     WRASFPRPKAIEILADSIRLCSLARPPHVAEAAQPA"
     gene            complement(14327..>14640)
                     /locus_tag="VIO83_08995"
     CDS             complement(14327..>14640)
                     /locus_tag="VIO83_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY8331447.1"
                     /translation="PASPQALPSWQSRLLAHLNRHKRYPEDARRRGQQGVVKLRFVVD
                     GRGQVLSFALAGKSGSASLDRATLQMIRRAQPLPVPPAELLQDGRLEVVAPFAYSLER
                     R"
BASE COUNT         2326 a         4860 c         5153 g         2301 t
ORIGIN      
        1 tgttcgatgt gcagctgaac atcccgcgca accgcgtcac cgccttcatc ggcccgtccg
       61 gctgcggcaa gtcgaccctg ctgcgcacct tcaaccgcat gaacgacctg gtcgacggct
      121 gccgcgtcga tggcgagatc cgcctcgatg gcaaggatat ctacgccagg ggcgtggaag
      181 tggccgagct gcgccgccgc gtcggcatgg tgttccagaa gcccaacccg ttccccaaga
      241 gcatctacga gaacgtggtc tacggcctgc gcctgcaggg catcaaccag aagcgcgtgc
      301 tcgacgagac cgtcgagtgg gcgctgaaga gcgcggcgct gtgggacgag gtcaaggacc
      361 gcctgcacga ctcggcgctg ggcctctccg gcggccagca gcagcgtctg gtgatcgccc
      421 gtaccgtggc ggtgcagccg gaagtgctgc tgctcgacga accctgctcg gcgctcgacc
      481 cgatctccac cctcaaggtc gaggagctga tcaacgagct caagggtcag tacaccatcg
      541 tcatcgtcac ccacaacatg cagcaggcgg cgcgcgtctc cgactacacc gcgttcatgt
      601 acatgggcaa gctgatcgag ttcggcgaca ccgacacgct gttcaccaat ccggcgaaga
      661 agcagaccga agactacatc accggtcgct acggctagtc cgaatgcgcg gggtgcgccg
      721 tgcgcaccag ggagcggtgc gcgcggcgca ccctacagtc agcttttgcg gagcgaatcc
      781 atgatcaaca aagacggcct tacccatcac atctccgccc agttcaacgc cgagctggag
      841 gagatccgca gtcacctgct ggccatgggt ggcctggtgg agaaacaggt caacgacgcg
      901 gtctccgcgc tggtcaccgc cgactccggc ctggctgcgc aggtgcgcgc ggtggacggc
      961 cagatcaacc agatggagcg tgacatcgac gaggagtgcg tgcgcatcct cgcccgccgc
     1021 cagccggccg cctccgacct gcgcctgatc atcagcatct ccaagtcggt gatcgacctc
     1081 gagcgcatcg gcgacgaggc caccaagatc gccaagcgcg ccatcgagct gtgcgagtcg
     1141 ggcgaggcgc cgaagggcta cgtcgagatc cgccacatcg gcgagcaggt gcgcaagatg
     1201 gtccaggagg cgctggacgc cttcgcccgc tacgacgccg acctggcgct gtcggtggcc
     1261 cagtacgaca agcaggtcga ccgcgagtac aagagcgccc tgcgcgagct ggtcacctac
     1321 atgatggaag atccgcgctc gatctcccgc gtgctcagcg tgatctgggc gctgcgttcg
     1381 ctggaacgca tcggcgacca cgcgcgcaac atcgccgaac tggtgatcta cctggtgcgc
     1441 ggcaccgacg tgcgccacac cggcctggcc aagcagggcc cggccaaggc cgacgacagc
     1501 caggcctgac cgcgctggcc cgcctcccgg actgacgggg cgcgggctgg ccatcgccgc
     1561 ccgccgaact gacctgcgtc aagccggcga cagtcagccg cgagaccggt cacaacccgg
     1621 ccgcccgctg ctcgctgagg gggaggccgg ctgctagtct tgcctcggta atctcctcat
     1681 tactgacgcc gtgacatcat gctgaatcgt ccgatcctgc tgactctcct gtctttccta
     1741 ggcctggcgc tggccgctcc ggccagcgcg cagaccgtgt acaagcacgt cgatgccaat
     1801 ggccaggtca gctacagcga ccaggcccgg ccgggcgcac aggtattcca gtttgccaag
     1861 cccggcgcgg cctcgcgcct gccgcggctc agagaggagc tggcgcgcca ggtgcatctg
     1921 gatactctca agcatgccgg tggcgatacc ctgcgggtgc gcaacgacct gttcgccccg
     1981 gtggagatcg aactgcgcct ggaggacgtc gccaacgcca gcggtgcgcc ggagcggccg
     2041 ctgcgctggg tgctgccgcc gcgcagccag atccgtctgc tgaccctggc gccgcgcgat
     2101 ccggcgcagc cgctgcgcta cacgccgaaa ctcagccacg ccctcggcga tccgcgcctg
     2161 ctacccaagc cgcatcgcta tccgctgccg tggagcggcg ggccgttccg ccagagccag
     2221 ggcgccaacg gccagtacag ccactttacg gccaagggcc gctacgccgt ggacatcgcc
     2281 atgccggaag gcacgccgat ccgcgccgcg cgcgaaggcg tggtggtgag cacctacaac
     2341 gaccaggccg gtgccaagcc cgagccgggc ggcaaccatg tgcggatcct ccacgacgac
     2401 ggcaccatgg gcgtctacct gcacctgcag cagggttcgg tgcaggttgc ctccggccag
     2461 cgggtgcgcg ccggcacgcc gatcgcccgt tccggcaaca ccgggcgcag cagcgggccg
     2521 cacctgcact tcgtggtgca gcgcaatgtc ggtctggcgg tggagtcgat ccccttccgc
     2581 ttcgcccagc cggtcaacag ccggccggag ctcgccgtcg gcggcgagga ttgaggcgcc
     2641 atcgcccgca cgccgctgcg gcggatctgc tgctcgagcg gcaatgaaca gggccacccc
     2701 gcggggtggc cctgttcgtt tgcgccggcg tcggggcgtg atcagtggag cttgagcacc
     2761 ttggccagca ggatcttcgg ccccttcatc ttcttgacga tgatgcgcag gccatgggtc
     2821 tcgagggttt cgtcttccgt gggcaggcgc ttgagggtgt cgtagaccag tccggccagg
     2881 gtgtcggctt cgacctcgtc gaggtcgacc tcgagcaggc gctcgatctt gaacagcggc
     2941 gtatcgccgc gcaccagcag cttgcccggc tggtaggcga gcaggccgcg ctcggtcttg
     3001 cggtgctcgt cctggatgtc gccgaccagc acctcgagca cgtcctccat ggtcaggaag
     3061 ccgaccacct tggcgtcggc ttcctcgacc agggcgaagt gcgcgccgcc ctggcggaac
     3121 tgctcgagca ggcggccgag cggcatgtgc cgcgacaccc gctccagcgg gcgcagcagg
     3181 gggcgcagat cgaaggcttc cgccggccgc tcgccaccgg ccagggccag cagcaggtcc
     3241 ttgatgtgca gcacgccgat gaactgctgc tcggcgtcca gcaccgggta gcggctgtac
     3301 ttgtgccggc ggatctcgtc gaggatctcg tcgaggctgg catcgacgtc gaggcagacc
     3361 aggtcctcgc gcgagttggc ccagtcgacc acctccagtt cacccagctc catggccgag
     3421 gccagtacgc gcatgccctg gtcgctgggg tcgcgcgcgc gactggagtg caggatcagc
     3481 ttcagctcgt cgctgctgta gtggtgctca tggtgcggcc ctggctcgcc ctggccggca
     3541 atgcgcagga tggcgttggc gctggtgttg agcacccaga tcgccgggta catggcccag
     3601 tagaacaggt acagcggcac ggcggtccac agcgacagca gctcgggctg gcggatcgcc
     3661 catgacttgg gcgccagttc gccgaccacg atgtgcaggt aggaaatggc gaagaaggcg
     3721 ctgaagaagg agatgccgtg caccagcgcc ggcgagtcga tgccgagctg ccccagcagg
     3781 ggttcgagca ggtgggcgaa cgccggctcg ccgacccagc cgagaccgag cgaggcgagg
     3841 gtgatgccca gctggcaggc cgacaggtag gcatcgagct ggccgtgcac ggtgcgcagg
     3901 atatgcccgc gccagccgtg ttcggcggcg atggtctcga cgcgggtgga gcgcagcttg
     3961 accatggcga actcggcggc gacgaagaag ccgttgagca ggacgaggag cagggcgaag
     4021 agaatgaggc cgaagtcggc gaagtagctg aacagggtgg cgctggggga ggggtccatg
     4081 gacggtcttg tgtcggtaat gaaggctata ggatgggatg tggcggcgca cctttcaagt
     4141 ccgccgggct ccgcgcacct tgcgccgcgg cggggcaggc gccattccga cgcccgctgc
     4201 ccgcgcagtc cacgcggggg cggctcagtt gcgcaccacc tgcgccggcg ggaagtggca
     4261 ggtgaaggtg ctgcccttgc ccatcgtgct gtcgatcttc agctggccac cgtggtggat
     4321 cagcacgtgc ttgacgatgg ccaggcccag gccggtgccg ccggtgttga tcgagcggct
     4381 ggcgtcgacc cggtagaagc gttcggtcag gcgcgggatg tgcttggcct cgatgccgat
     4441 gccgctgtcc tgcacggcca ggtggccgcc gctctcgtcg gcccaccagc ggatgcggat
     4501 ctcgccgcct tccgggctgt acttgaccgc gttgaacacc aggttggaga aggcgctgcg
     4561 cagctccgac tcgctgccct tgagcttcac cgccggatcg gcctccaggc tgatgcggtg
     4621 gcggtcggcg gacagcgcct gggcgtcggt gcggatgctc agcagcagca ggtcgaccgc
     4681 caccggccgg ctgtcggacg ggtagggggc ggcctccagg cgggcgagca gcagcaggtc
     4741 gttgagcagg ttctgcatgc gcgaaccctg ctgctgcatc tgctgcaggg cgcggcgcca
     4801 gcgcggattg acctcgtcga cattgtccag cagggtttcc aggtagccgg tgatcaccgt
     4861 cagcggcgtg cgcagctcgt gggagacgtt ggcgacgaag tccttgcgca tctgctcgag
     4921 ctggtagacg cgggtcacgt cgcgcaccag catcaggtgc tcgccgttgc cgtagagggt
     4981 cagctggaac tgcaggcgca catggtcgtt gaccggcgag ggcagctcca gcggctcgcg
     5041 gtggttgtcc tgagcgaaat atttcttgaa gtgcgggtgg cgcaccaggt tggtgatcgg
     5101 ctggccaccg tcctgcttgc gcttgaggcc gagcagggtg ccggcggcgc ggttccacca
     5161 ctcgaggttg ccctggcggt cgagcatgat caccgcatcc tgcagcgccg cggtcgactc
     5221 ctgcacgcgg tcgaccaccg cctgcaggcg gccacgggcg tgctggttgc gcttctgcag
     5281 ctggtagatc aggtcgtaca cctcgcccca cagccccgaa ctttccggcg gcaactcgtc
     5341 cgaggcgcgc ttcagccagt cgtgcaggcg caacagctgg cgcaggcacc agaacaggta
     5401 gccggccagg ccgaatgcca gcgcccaggc atactggccc gtgatcaggc cgagcagcag
     5461 acagccgccc agcagcaaca gcaggcggcg ggtcagcgcg ccgcgccagt cgtggttcac
     5521 cttgcagtcc ttgtaaagcg gccgcgctca gcggcagaac gagttgtcgc gccgtgccgg
     5581 cttcagccct tggtggagaa acgatagccg gtgccgcgta ccgtctgcac cagattttcg
     5641 caggtttcgc cgagggcctt gcgcaggcgg cggatgtgca cgtcgacggt gcgctcctcg
     5701 acatagacgt tgccgcccca cacctggtcg agcagctggc tgcgggtgta ggcgcgctcc
     5761 tggtgggtca tgaagaactg cagcaggcgg tattcggtcg gccccatctc ggccgccttg
     5821 ccgtcgatgg tgacgcggtg gctgaccggg tcgaggatca acccgccgat ctcgatcggc
     5881 gtctcggcat cgctggcgcc ggcacggcgc agcacggcct tgaggcgggc gaccagctcg
     5941 cgcggcgaga acggcttggt gatgtagtcg tcggcaccga cttccaggcc ctggatcttg
     6001 ttgtcctcct cgcccttggc ggtgagcatg atgatcggcg tgtcggcggt cagctcgtcg
     6061 cgcttgaggc ggcgcgccag ctcgatgccg gtggtgccgg gcagcatcca gtcgagcagg
     6121 atcaggtcgg gtttgcggtc gacgatcagc gcatgggcct gttggctgtt ttccgcctcc
     6181 aggcactcgt agccggccat ttccagggcg acggcgatca tttcgcgaat cggtgcttcg
     6241 tcgtcaacga tgaggatgga tttgccaacc atgggggctg tctctgtagt ccgtgggcat
     6301 gctgggcatt acataacgga attattgcag ctatgtgaca tgtggacggt ttgccggact
     6361 gcaggtcgtc cggccggcgc gatttcgccc tggcggggca cccgccggtg acggcggaac
     6421 cgctcgacta tgctgcaaca gcaggccggc ggttgtcggc aggccatccc tggtgcgtca
     6481 tgccgcgcag gcgcggtcgg cgccgggtcg tttcatccca cccgaggaag ctgcgatgct
     6541 gagaatctcc ccgaccctgc tggccgtggt cggcgccgcc accctggcgc tgctgggaca
     6601 ggtccaggcc gccgaaccgg cggcagtcca ggacggcatg ctggtcgatg ccaagggcat
     6661 gaccctgtat accttcgaca aggacgccat gggcaagtcc agctgcaacg acagctgtgc
     6721 gaccaactgg ccgccgctgg ccgccagtgc ggatgccgac gagggtgacg actggacggt
     6781 gatccagcgc gccgacggca gctggcagtg ggcctactac ggcaggccgc tgtacacctt
     6841 cgcccgggac cagaaaccgg gcgacatgct cggcgacggc aagatgaacg tctggcatgt
     6901 cgccaggccc gaggcggagg acgccggcga ccagtagccg gcccggcccg ctccgacgcc
     6961 cggcgccgga gggtcgcgcg ttagcgcagg gcgtagtcgg cgatggtgcc ggcgaggatc
     7021 gccaggccgg cccagtggtt gtgcaggaag gcgcggaagc aggcgtggcc gtcgcgcgtg
     7081 cgggtgctcc agtattccca ggcgaagcag gccgccgccg ccgccaggcc gaggtggaag
     7141 tacaggccca ggccgaagcg cccggcggcc agcagcaggc acagcaggct gaggccctgg
     7201 aggatggcga tgatcgggcg gtcggcgtcg ccgaacagga tggcggtgga cttcacgccg
     7261 atccgcaggt cgtcgtcgcg gtcggtgatc gcgtagtagg tgtcgtaggc caccgtccac
     7321 agcaggttgg ccaggtacag caggccggcc gccgccggca ggctgccggt ctcggcggtg
     7381 aaggtcatca ggatgcccca cgaataggcc gcgccgagca ccacctgcgg gtagtaggtg
     7441 tagcgtttca tgaacgggta cagcgcggcc agcgccagcg cgccgcacga cagccagacg
     7501 gtgatcgcgt tggtcagcag caccagcccg aaggccaccg ccatcagcac ggcgaacacc
     7561 cgcagcgcct cgcgcgcggt catccgcccg ctggccagcg ggcgggcgcg ggtgcgctcg
     7621 acgtggccgt cgacgcggcg gtcggcgaag tcgttgatcg cgcagccggc cgagcgggtg
     7681 aggatcacgc cgagggtgaa gatcagcagg ttggccagcg acggcacgcc gcctgcggca
     7741 atccacagcg cggtgagggt cggccacagc agcaggtaga tgccgattgg ccggttcagg
     7801 cgggtcaggt cgatgaagtc ctgggcgcgc ggatgcaggc gggccagggg cttgagcagg
     7861 ctgagggaca tcgcggatct ccgcaggtga aaccatccgg ccgccgccgc gcgtgcggcc
     7921 gggcaaaggg cgccagtata aggcgtggcg ccaggcaggg gtggttcagg tgtgcggcgt
     7981 ggcgtccgcc tgccacagcg ccggcaggaa gacctcggcc accagtacgc ccagcgcccc
     8041 ctggcagaac agcgagcggc ggccccacag atctgccgtg cgtaccgccg cgggcagcag
     8101 cgcggccgga tagcggcagg cctcgatcgg ctggcgggtg aaggccggat cgcggaacag
     8161 caggtggccc agcgacgtgg tgccgagctg gtcgagggcg aagggatgca gctccagcct
     8221 gctgcgcgcg gccacgctgc gggcgaacac ccagggcagg ccctggccgc gcaggtagac
     8281 ctcgcgcacc cagccctcgc tgccgggcgg cacgccgagg gcagcgcatt cgtcggcgcg
     8341 caggatctgc cagccctcga ccagcggctc gacggcgaag gtgtcgccgc tgagggcggt
     8401 gaggcggcgg gtcagcgaac cctgctcgtt gagccagtcg agttcgcggg cgttgggcgt
     8461 ggggtgcagg tggtcggtgg ccagccactc ggggctggcg caggcagtct gggacacggc
     8521 gggattccgc agcgaaaaac cggctgccag tctagcatgc accttccggc cttgccgacc
     8581 ggcgcatggc ggggcgggct gccttgccgt gccgctggtc cggcgtcgcc ctctacgacc
     8641 attggccggc tccgggccgc tggcgcgtgt cggtccggcg tgccattctt cagcggtcag
     8701 ccgcagccca gagcctgtcc ggcgtctctg cagcctcgat ggcgcaggtg gcgggcggtg
     8761 gcaacaggga gacgcccatg ccgggcagcc cgcacaacaa gaacaaaccc gagaggcacg
     8821 tatgcgtaaa ccggaactcg ctgccgcagt cgcggcaaag gccgacctga gcaaggaaca
     8881 agccagccgc gtactcaacg cggtgctaga agaaatcacc agtgcgctga accgtgagga
     8941 cagtgtcacc ctggtcggtt tcggcacctt cctgcagcgc catcgcgggg cgcgcacggg
     9001 caagaacccg cagaccggcg agccggtgca gatccgcgcc agcaacaccg tctccttcaa
     9061 gccgggcaag gcgctgaagg acgcggtcaa ctgagtgcgc cgacccggcg tcgttcgact
     9121 ccgggtcgtt gaagccggtc gagcaatccg tggttcaggt ggtcttcggc cggcgcgcga
     9181 cgcgctagaa tccaggttcc ctgtgcgtga ctcagaggtt ctgccgtgaa attccgcttc
     9241 ctgctctggc tgctaggccg cctgctggcc ggcgccagcc gtaccaacgc cgagttccgc
     9301 gagcaactgg tcgatcgcga cctggtgttc cagctgcaga cccaggacgg caagatcgct
     9361 cgccatttcg tggtcaagga ccagcgcatc cgcagcgcca ccggcagcgt agcgcatccg
     9421 gcgttcagca tcgccttccg tgacagtgcc tacgccttcg ccaccctcac ggcggcgaac
     9481 aagcagctgg ccttcatgca gggcatccag aaccgggaca tccagatcca gggcaacccg
     9541 gcgctggtga tctggttcca gggcctgacc cagctgctcg gcccacggca gaagcaggcg
     9601 gcctgagcgt catccgctcc tggccggaag ccttgccggg ggcgtcatgc gcacgtgggc
     9661 agcctccagc agtcacacct cccgcacctc tgccctcggc aaacacccgc tggccttcac
     9721 gtgcgcatat ctaccgtcac ggctaactct cggggtcagc aatggaaatc ctgattctga
     9781 tcggcctgat cgtgctgaac ggcctcttcg ccatgtccga aatcgcgctg gtcacggcgc
     9841 gcaaggcacg cctcatgaag ctggcggccg acggcgacaa ttcggcgtcc gtagcgctca
     9901 agctgggtga agaccccacc aagttcctgt cgaccatcca gatcggcatc acctccatcg
     9961 gtatcctcaa cggtatcgtc ggtgaggctg tactggccgg tccgttgtcg cagtggctgc
    10021 aatcgttcgg tattgcagaa aatgccgcca acatcggcgc gaccgcggct gtcgtcattg
    10081 tcgtgaccta cgtttccatt gtggtgggcg aactggtgcc caaacgcatt ggccagctca
    10141 acccggagcc cattgcgcgc ctggtcgcgc gtcccatgca ggtgctggcc ctgattaccc
    10201 gtccgttcgt catgctgttg acggtgtcca cccatgcact gctgcgcctg ttgggcgtca
    10261 agcagagcag ccaggccagt gttacggaag aagaaatcca cgccatgctg gaagagggct
    10321 ccgaggcagg cgtcatcgag cagcatcagc acgagatggt gcgcaacgtc ttccgcctgg
    10381 atgaccgcca actgggctcg ttgatgattc cgcgttcgga tatcgtctgt gtcgacatcc
    10441 ggcgctctcc tgaagagaac ctgcaagtcc tgatcgagtc agagcactcc cgcttcccgg
    10501 tctgcgatgg cagcctcgac aagctgctcg gggtcatcca cgccaaacag gccttggcgc
    10561 gtgtggcgaa gggtgagacc cccgactact ccgccaacct ccagccgtgc gtctacgttc
    10621 ccgaaacgct gaccggcaca gagctgctgg agcagttccg tgccaacgac atgcagatgg
    10681 ccttcgtcat cgacgaatat ggcgaaatcg aaggcattgt caccctgcag aatgtcctgg
    10741 aggcggtgac cggcgagttc accccgcgca atgccgaaga tgcttgggca gtccagcgtg
    10801 aggacggttc ctggctgctg gatggcgcca ttccgattcc ggagatgaag gatcgcctgg
    10861 aactcaagaa cctccccgag gaggacaagg gccgttacca caccatcagc gggatgatca
    10921 tgctgctgct cggacgggtg cccggcaccg gtaaccatgt ggactgggcg ggctggcggt
    10981 tcgaggtcgt ggatatggac gacaagcgga tcgacaaggt gctcgcgacg ccccttctga
    11041 atggcgatga aagcgaggcc tccgctcagg agtaagcctc gcttcactct gccatccatc
    11101 ctcagccggg gttggacggc aattgggacg gtgtgttgcc caaacgctca ctctctggca
    11161 cacaaaacta acgataaaaa accattatcg agagtttccg ccccagcagg cttcgctccc
    11221 cgcttcacac ctgcccatac tgctgcccat ggcgcagcca gcgctccagc agcggcgcgg
    11281 cgtgctgcgg ccagtgcgcg agcagggtct gcgcggcctc gcgtactgcc ggcagcaggt
    11341 cggcgtcgcg catcaggtcg gcgaccttga actgcagcag gccggtctgg cgggtgccga
    11401 gcatctcgcc ggggccgcgc agctcgaggt ccttctcggc gatcacgaag ccgtcgctgg
    11461 tctcgcgcat gatcgccagg cgctcgcggc ccatgtgcga cagcggcgcg tgatagagca
    11521 gcacgcagtg gctgaccgcg ctgccgcggc cgacccgccc gcgcagctgg tgcagctgcg
    11581 acaggcccag gcgctcgggg ttctcgatga tcatcaggct ggcgttgggt acgtccacgc
    11641 cgacctcgat caccgtggtg gcgaccagca gctgcaggcg cgcggccttg aactcctcca
    11701 tcaccgcggc cttctccgcc ggcttcatgc ggccgtggat cagaccgacg cgcagctcgc
    11761 ccagcgcggc ggacagctcc tcgaaggtgg tctgcgctgc ctggcaggtc aactcctcgg
    11821 actcctcgat cagcgtgcac acccagtagg cctggcggcc ctctgcgcag gcggcgcgca
    11881 cccgctcgat cacttccggg cggcggctgt cggcgatcac cagggtggtc accggcgtgc
    11941 gccccggcgg cagctcgtcg aggatcgagg tgtcgaggtc ggcataggcg ctcatcgcca
    12001 gggtgcgcgg gatgggcgtg gcggtcatga tcagctggtg cgggcacagc cggccgtcga
    12061 cgcccttctg gcgcagcgcc agacgctgct gcacgccgaa gcggtgctgt tcgtcgatga
    12121 tcgccagtgc caggcgctgg aacttcacct cgtcctggaa cagcgcgtgg gtgccgatca
    12181 ccatcggcgc gccggcggcg atgcgctcca gcgcgctggc gcgcgccttg cccttgagct
    12241 tgccggccag ccaggcgacc tcgatgccca gcggctcgag ccacttgccg aagttgagga
    12301 agtgctgctc ggcgaggatc tcggtgggcg ccatcagcgc cacctggtag ccggcctcga
    12361 tggcctgcag cgcggccagc gcggcgacca ccgtcttgcc ggcgccgacg tcgccctgca
    12421 ccaggcgcag catcggctcg tgttgctcta ggtcccaggc gatctcgccg gccacccgct
    12481 gctgggcgcc ggtcggctgg aagccgaggt tggcgaggaa ctgcgccggc agacgtttgg
    12541 ccttggccag cggtggcgcc tgctgggcgc ggatgctctc gcgcaggcgc tgtagcgaca
    12601 gctggtgggt cagcagttcc tcgaaggcca ggcggtgttg cgcccagtgg cggccctcgg
    12661 ccagctcctc gaggtcggcg tccggcggcg gccggtgcag gtagcggatg gcgtcggcca
    12721 gcggcgcgag gtcgtactgg cgggccagct cggccggcag ccagtcgggc aggctgtggc
    12781 cgttgaggct gcccagcgcc tgctggctga gggcgcgcag gcgctgctgg gtgaggccct
    12841 cggtgctcgg gtagatcggc gtgaggctct gctcgaccgc cgccgacggc tcgtcggcgg
    12901 ccagggcgcg gtactcgggg tggtagatct ccagtccggt ggcgccggtg cgcacctcac
    12961 cgtagcagcg caggcaggtg ccgcgcttga gcgcgtcctt ctgcgcctgg ctgaagtgga
    13021 agaagcgcag cgacagggtg ccgctggcgt cctgcaggcg caccagcaga ctgcggcggc
    13081 ggccgaggac gatgtcggcg ccactcacca cgccttccac caccgcgtcg ccgcccgggc
    13141 gcagggcgcc gatcgggctg acccgggtgc ggtcctggta gcgcagcggc aggtggaaca
    13201 gcacgtcctg caggctttcc aggccgacct tggccagctt ctcggccagt gccgggccga
    13261 cgcctttcag tgtggtgacg gcgatccgcg ccagttcggt catgccggct gcgcagcctc
    13321 cgcgacatgc ggcgggcggg ccagcgagca caggcggatc gagtcggcga ggatctcgat
    13381 ggccttgggc cgcggaaagc tggcgcgcca ggcgatcgcc acggtgcggt agggcaccgg
    13441 cggggtcagc ggacggatgg cgatcacgcc cggtgcgtag tggtggctgt ccaccgcagt
    13501 gaacggcagt accgaaatcc ccagcccaga cgccaccatg tggcggatgg tttccagcga
    13561 gctggactcc acggtggtgt gctgctgccc ctcgctgccc ttgcgggtgg tcgggcaggc
    13621 ttccagcacc tgatcgcgga agcagtggcc ttcgccgagc agcagcaggc tcttgtcgtt
    13681 gagcatggcg ctgtcgatgg tctgccgcgc ggtccacgga tggtcggccg gcatcaccac
    13741 gaagaacggt tcgtcgtaca gcggcttggt cagcacgtcg gcctcggcga agggcagggc
    13801 gatgatgatc gcgtccagct cgccggtgcg cagcttgtcg cgcagcacgt gggtgaagtt
    13861 ttcctcgatg tacagcggca tctgcggggc gacccggtgc agctgcggga tcaggtgggg
    13921 gaacaggtag gggccgacgg tgtagatggc gccgaccttg agcggtgccg ccagctggtt
    13981 cttgccggcc tgcgccagct cgcggatgcc ctgggcctgc tccagcacct tctgcgcctg
    14041 ggcgacgatg ccttcgccga ccggggtcag gcgcacggcg ctcttgctgc gctcgaagat
    14101 cagcacaccc agctcgtcct cgagcttctt caccccgacc gacagggtcg gctggctgac
    14161 gtggcagcgc tcggcggcgc ggccgaagtg ctgctcctgg gcgagagtga cgatatagcg
    14221 cagttccgtg agtgtcatag cgaatatcca ttgagatgcc cccaagcata gcggctgtct
    14281 tcaatggaac caaccgcccg cgcgccggcg ggcggctggc ggaccctcag cgccgctcca
    14341 gggagtaggc gaagggcgcc accacttcca ggcgaccgtc ctgcagcagt tccgccggcg
    14401 gcaccggcag tggctgggcg cggcggatca tctgcagggt ggcgcggtcc aggctggcgc
    14461 tgccggactt gccggccagc gcgaaggaca gcacctggcc gcggccgtcg accacgaagc
    14521 gcagcttgac cacgccctgc tggccgcgcc ggcgcgcatc ctcgggatag cgcttgtggc
    14581 ggttgaggtg ggcgagcagg cggctctgcc agctgggcag ggcctggggc gacgccgggc
//
LOCUS       DATCUN010000235         9430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_10126, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000235 DATCUN010000000
VERSION     DATCUN010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9430
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10126"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VIO83_09000"
     CDS             complement(<1..436)
                     /locus_tag="VIO83_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative coupling factor TraD, PFGI-1 class"
                     /protein_id="HEY8331448.1"
                     /translation="MSGKQPVEVLLRPAVELYTVAACAGAAFLSLVAPWSLALSPAMG
                     VGSALAFGAYGAIRYRDARVILRYRRNIRRLPRYVMTSKDVPVSQQRLFVGRGFLWEQ
                     KHTHRLMQTYRPEFRRYVEPTPAYRLARRLEERLEFAPLPLSR"
     gene            complement(441..989)
                     /locus_tag="VIO83_09005"
     CDS             complement(441..989)
                     /locus_tag="VIO83_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HEY8331449.1"
                     /translation="MTTSHLAHLSVTGLLVLLAGLPLASLASEPLIVVEERGGTSALP
                     YYEALSLQPRANTPARSPIPATQIPATPADEAAMLPVRSAKLTPGTVARRVIEAPGLR
                     PFVVVGDDEASQAWLRRHAASLRERGAVGLVVNVETVQGLARLRALASGVPLAPVSGD
                     DLAERLGLRHYPALITATGIEQ"
     gene            complement(986..1576)
                     /locus_tag="VIO83_09010"
     CDS             complement(986..1576)
                     /locus_tag="VIO83_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY8331450.1"
                     /translation="MAAPAIAALLRALALAAGLCACVAQAQEVPPPAYQLAAQRAGIP
                     STVLYAVALQESGIRRNGRIVPWPWSLNVAGQSRRYATRADACAGLQRAMRSTPHTRI
                     DAGLGQINLGYHHQRYASACDLLDPYRNLAIAAEILREQHTPGEDWLLAIGRYHRPAG
                     GEPAARYRRSVSRHLARVQGTRPTAAVLAARQETSP"
     gene            complement(1558..2283)
                     /locus_tag="VIO83_09015"
     CDS             complement(1558..2283)
                     /locus_tag="VIO83_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003120008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HEY8331451.1"
                     /translation="MKPSIILSALLLASAQWPVWAQQPPAAPARNAQSQERPLVARVL
                     DDRVASDWGLQPQEWARYRELMDGPLGIYSPNLDPLSALGIEARTDEERRRYAELQVQ
                     VEARRVEKLLAYQRAYDEAWQRLNPGMQRVNLPDDKPVAGATRGSGRTAVFVKDGCDA
                     CGQLVQRLQSSGAEFDLYMVGSRQEDARIRDWAKRANVDPARVRRGTITLNHDGGRWL
                     SLGLPGDLPAVVREVNGQWQRQP"
     gene            complement(2298..2939)
                     /locus_tag="VIO83_09020"
     CDS             complement(2298..2939)
                     /locus_tag="VIO83_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331452.1"
                     /translation="MNAPQPAQRSTAAVVVQSLMWLWLIGLSVLVALGYQAMNDQADQ
                     DRLDSRLQRLEAQATGLAETVEAIQQRPAVATAADLKDTRQILEARAAQVEQSLSAYA
                     AADDLQALRAEVEQVKARQTAARAAVPAQPRAPSKSAAKPEPPPLPFRIVGAELRAGE
                     RSVSVAPSSGDFTPDQLQVLLPGDAAGPWHLQAVEGNTAVFQAGDQTRRVAIP"
     gene            complement(2936..3535)
                     /locus_tag="VIO83_09025"
     CDS             complement(2936..3535)
                     /locus_tag="VIO83_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HEY8331453.1"
                     /translation="MCPSPPWFHHPERRLLAGFLGLLWSVLAGGCATTSAPVAPDTIE
                     EVSAAPAPEAPEYIPVVRYGRYTLVELAPTAAQRDLLLQTIDVSMPEDARATVGDGLR
                     HVLKRSGYGLCQTAHAVIELYTLPLPAAHLHLGPMTLRDALLTLAGPAWELHTDDRAR
                     QICFERPGDSVTADTAPAPPATEAVQTFPLAPAASGGQP"
     gene            complement(3694..4563)
                     /locus_tag="VIO83_09030"
     CDS             complement(3694..4563)
                     /locus_tag="VIO83_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331454.1"
                     /translation="MNATTQATPETVNARLTLDVTYLLNGEAAPELLARLERMCERAI
                     GEGLLTGETAAEVDTWSIDVAVLPSETDRDALEAKIAAFMQQRIEDGNLGAEDIPVRL
                     ARYGLMAPEAFADEMRERMGMDDDAPTGRAVIPSTEPADGSEIEMQVAVACIGASGSP
                     DVPVFKVSITQEEYDLGIHYDKARDLAEEARYEGPFVCFDAAEYGPILSAARELGLVP
                     QVVVVDMTDGQIHSIRCDSGEIKVICYDTSDTEEYSAAVANRPLGENGQLVRCWSHTQ
                     LAQVDPGLKLARD"
     gene            complement(4678..6960)
                     /locus_tag="VIO83_09035"
     CDS             complement(4678..6960)
                     /locus_tag="VIO83_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015879477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY8331455.1"
                     /translation="MNAQVIDAASAADTSSPMDDLLEQEPSELSMSLQDFVSEFGDEL
                     LESLNRANPPVYSGTPRPHRQLVLAGLKRQLFPAQAEVVHAASELLVNQGERAVFVNG
                     EMGTGKTTIGIALAAVLHAEGYRRTLVLSPPHLVYKWRREILETVAGAKVWVLNGPDT
                     LLKLIKLREQLGVPSPAQEFFVLGRVRMRMGFHWKPAFARKHTRHGDVGACPHCGEVV
                     TNLDGEPITAQELEAEEFRRKCNGCGGALWTLMRPRSLSGSDQSSAVLRALKRIPTIG
                     EATAQKLMKTFGDSFLASMLGDNLHNFINLMDASGKLVFSDRQAHRMERSMANMEFGF
                     GEGGYQPSEYVKRYLPRSTFDLLIADEAHEYKTGGSAQGQAMGVIASKVRKTVSLTGT
                     LMGGYADDLFHLLFRALPGRMIEDGYIPGRNGSMNAAAMAFMRDHGVLRDIYSESDGP
                     AHKTAKGSKVTVRTVKAPGFGPKGVLRCILPYTIFLKLRDIGGVLPPYDEEFRDVAMS
                     AEQGEAYQKLASSLTQQLKEALRKRDTTLLGVVLNTLLAWPDCCFRAETVRHPRTREV
                     LAFTPAQFNELEVMPKERELINICREEKEAGRKTLVYSVYTGTRDTTSRLKMLLEQEG
                     FKVAVLRASVDASQREDWIAEQIDRGIDVLITNPELVKTGLDLLEFPTIVFLQSGWNV
                     YTLQQAARRSWRIGQKLAVKVIYLGYAASSQMTCLALMAKKILVSQSTSGDVPESGLD
                     VLNQDGDSVEVALARQLVAA"
     gene            complement(7078..7377)
                     /locus_tag="VIO83_09040"
     CDS             complement(7078..7377)
                     /locus_tag="VIO83_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331456.1"
                     /translation="MSTVAQHAFPRFNPGQVVMTSGVASLVQQGQLNPRPYLLRHLSA
                     DWGDLDDSDRRQNDAALKSGEARLFSAYEVGPSLKVWVITEWDRSVTTVLLPSEY"
     gene            complement(7477..7794)
                     /locus_tag="VIO83_09045"
     CDS             complement(7477..7794)
                     /locus_tag="VIO83_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012438488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY8331457.1"
                     /translation="MLSIFQGRKPQVPPLFAPGTLKLSEKVHWLASKSLIDPLPYVQR
                     HVRGDWGEASEAECQLNDVALEQGAPMTSRFKITPRLFLLVITSDDQCTTVVQLPEEG
                     AVN"
     gene            complement(7846..9000)
                     /locus_tag="VIO83_09050"
     CDS             complement(7846..9000)
                     /locus_tag="VIO83_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015879474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6094 domain-containing protein"
                     /protein_id="HEY8331458.1"
                     /translation="MACFRSSSPPDRRLPMALMFPRLARNFAKNGYYPTDEATLERTL
                     SALAPSDGPMCICDPCAGEGVAIAEAAHALGPELASAYAVEFDQERATHARLLVDHCI
                     HGDLMDTMISRQAFGLLWLNPPYGDLARDTSGNLGYEGKGRARLEKLFYQRTLPLLQY
                     GGILVFILPSYVLDQEMVGWLTRHFADLSVFQAVDKQFKQVVVFGRRVRQRDQAGDDV
                     KASRARLLQIGLAELEPDELPPDWTLLPYVVPTAPAEPEHFYRISMEPEQFADEVRRL
                     KGLWPAFETHLGATQQTLRAPARALSQWHLALALAAGAISGVVQSPSGHTLAVKGDTF
                     KQKSSAVEYRERDDGSLAETRILTDKFVPVIRAWDLTPSSSTLGQVLTIH"
     gene            complement(9021..>9430)
                     /locus_tag="VIO83_09055"
     CDS             complement(9021..>9430)
                     /locus_tag="VIO83_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331459.1"
                     /translation="RLEKRLTRSYRRTLFGSMVNLWLFDRRCIRPDKSSASALALLPR
                     SAADPTSRLWHLVKDTCPLPLLDHWQSPVLALLREREMLCSLRMALGPLQGFRLAIDV
                     PALTNALGDLIRQGVLTAYPYPARIWTPEPAAV"
BASE COUNT         1543 a         3003 c         3328 g         1556 t
ORIGIN      
        1 gccgtgacag tgggagcggc gcgaactcca gccgttcctc caggcgccgc gccagtcggt
       61 aggccggcgt cggctcgacg tagcgccgaa actctggccg gtacgtctgc atgagcctat
      121 gggtgtgttt ctgctcccac aaaaagccgc gccccacaaa cagacgctgc tggctgaccg
      181 gcacgtcctt gctggtcatc acatatcgcg gcaggcggcg gatgttgcgt cggtagcgaa
      241 ggatgacgcg ggcatcgcgg tagcggatcg cgccgtaggc gccgaacgcc agtgcgctgc
      301 cgacgcccat ggcagggctc agcgcgagcg accacggggc caccagggac agaaacgcgg
      361 cgcctgcaca cgccgcgacg gtatatagct ccaccgctgg gcgaagcaga acctcgaccg
      421 gctgtttccc cgacatggcg tcattgctcg atgccggtgg ccgtgatcag cgccgggtag
      481 tgccgcaggc ccaagcgctc ggctaggtcg tcgccggaca cgggcgcaag cggcacgccg
      541 gacgccagtg cgcgcagccg cgccaggcct tgcacggtct cgacgttgac caccagaccg
      601 accgcgccgc gctcgcgcaa tgaggctgca tggcggcgca accaggcctg ggaagcctcg
      661 tcgtcgccca cgaccacgaa gggacgcagg cccggggcct cgatcacccg ccgcgcgacg
      721 gtgccgggcg tgagcttggc gctgcgcacc ggcaacatcg cggcctcatc cgccggcgtg
      781 gccgggatct gagtcgcagg gatgggcgat cgggccggag tgttggcgcg cggctggagg
      841 ctcagggctt cgtagtacgg cagcgccgac gtgccgccgc gttcttcgac cacgatcagc
      901 ggctcgctgg cgagagaggc cagcggcagg cctgccagca gcacaagcag acccgtcacc
      961 gagagatggg ccagatggga tgttgtcatg gggatgtctc ctggcgcgcg gcaaggaccg
     1021 cggcggttgg gcgcgtgccc tgcacgcggg caaggtggcg ggatacgctg cgccggtagc
     1081 gggcggcggg ctcaccccct gcaggacggt ggtaacggcc aatcgccagc aaccagtctt
     1141 cgccgggggt gtgctgctcg cgcaggatct cggcggcgat ggccagattc cgatacgggt
     1201 cgagcaagtc gcacgcgctg gcgtagcgct gatggtgata gccgaggttg atctggccca
     1261 ggcccgcgtc gatgcgcgtg tgcggcgtgg agcgcatcgc ccgctgcaag ccggcgcagg
     1321 cgtcggcgcg ggttgcgtag cggcgcgatt ggccagcgac gttgagcgac cacggccacg
     1381 ggacgatgcg cccattgcgg cggatgccgc tctcctgcaa ggccacggcg tagagcaccg
     1441 tcgaggggat gcctgcgcgc tgcgcggcga gctggtacgc cggtggcgga acctcctggg
     1501 cctgggcgac gcaggcgcac aggccagcgg cgagcgccag tgcgcgcagg agtgcggcta
     1561 tggctggcgc tgccattggc cgttcacctc gcgcacgacc gcgggcaagt cgccgggcag
     1621 gcccagcgac agccagcggc cgccgtcgtg gttgagcgtg atggtgccgc ggcgcacgcg
     1681 cgctggatcg acgttcgcgc gtttggccca gtcgcggatg cgcgcgtctt cctggcggct
     1741 gccgaccatg tacaggtcga attcggcgcc cgaggattgg aggcgctgca cgagctggcc
     1801 gcaggcatcg cagccgtcct tgacgaacac cgccgtgcgg ccggagccgc gcgtggctcc
     1861 ggcgaccggc ttgtcgtctg gcaggttcac gcgctgcatg ccggggttca ggcgctgcca
     1921 ggcctcgtcg taggcgcgct gataggcaag caacttctcc acgcggcgcg cttcgacctg
     1981 cacctgtagc tctgcgtagc ggcgccgttc ctcgtcggtg cgggcctcga tgcccagggc
     2041 cgatagcggg tccaggttgg gcgagtagat gcccagcggc ccgtccatca gttcgcggta
     2101 gcgcgcccac tcctgcggtt gcaggcccca gtcgctcgcc acccggtcgt ccagaacgcg
     2161 agcgaccagc gggcgctcct ggctctgtgc attgcgggcg ggtgccgcag gcggctgctg
     2221 cgcccagacg ggccactggg cggacgccag caggagcgcg gaaaggatga tcgacggctt
     2281 catgcgatct gctccggtca gggaatcgcc acgcggcggg tctggtcgcc ggcctggaac
     2341 accgcggtgt tgccctcgac ggcctgcaag tgccacgggc ccgcggcatc gccgggtagc
     2401 agcacctgaa gctggtcggg cgtgaagtcg ccactgctcg gcgcgacgga cacgctgcgc
     2461 tcgccggcgc gcagttcggc accgacgatg cgaaacggga gcggcggtgg ttcaggcttg
     2521 gcggctgact tgctcggtgc gcgcggctgg gcgggtactg cggcgcgtgc ggcggtctgg
     2581 cgcgccttga cctgctcgac ttcggcgcgc agtgcctgaa ggtcgtcggc ggcggcatag
     2641 gcgctcagcg attgctcgac ctgggccgcg cgtgcttcca ggatttggcg ggtgtctttg
     2701 aggtctgccg ccgtggcgac ggccggacgc tgctggatgg cctcgacggt ctcggccaga
     2761 cctgttgcct gcgcttcgag gcgttgcaga cgggaatcga ggcgatcctg gtcggcctgg
     2821 tcgttcatgg cctggtagcc cagggccacg agaacactga ggccgatcag ccagagccac
     2881 atcaggctct gcaccaccac ggcggcggtt gaacgctggg cgggttgcgg ggcgttcatg
     2941 gctggcctcc cgatgccgcg ggcgccagcg ggaacgtctg caccgcctcg gtggcgggcg
     3001 gcgcgggtgc ggtgtcggcg gtcacgctgt cgccgggccg ctcgaagcag atttgccgcg
     3061 cgcggtcatc cgtgtgcagt tcccaggccg ggccagccag cgtgagcagc gcgtcgcgca
     3121 gggtcatggg gccgaggtgc agatgcgccg ccggcagcgg cagtgtgtac agctcgatca
     3181 ccgcgtgcgc tgtctggcac aggccgtaac cgctgcgctt gagcacatgc cgtagcccgt
     3241 cgccgaccgt ggcgcgtgca tcctcgggca tcgacacgtc gatggtctgc aacagcaggt
     3301 cgcgctgcgc cgccgtgggt gcgagttcca cgagggtgta gcggccgtag cgcacgacgg
     3361 ggatgtactc gggtgcctcg ggtgcgggcg cggccgagac ttcctcgata gtgtcgggcg
     3421 cgaccggcgc gctcgtcgtc gcgcagccgc cagccagtac cgaccaaagc aggccgagaa
     3481 atcccgccag caggcggcgt tcgggatggt gaaaccaggg tggagagggg cacatggcgc
     3541 gtcgtcctga aacatcgagc cctcaccatc gccgctcggg ccacgagcgg cagcaaacaa
     3601 tgcgaaccag gcagcgcccg atttcccggc agcctccctc caaagaaaaa ggcccccgaa
     3661 ggggccagtg aaaacgcatt ggacttcgct cgatcaatcc cgagccagct tcaggccggg
     3721 atcgacctgc gccagttggg tgtgcgacca gcagcgcacg agctggccgt tttcaccgag
     3781 cggccggttc gccaccgccg ccgaatactc ttcggtgtcg ctggtgtcgt agcagatgac
     3841 cttgatctcg ccgctgtcgc agcggataga gtggatctgg ccgtcggtca tatcgaccac
     3901 gacgacctgc ggaacgaggc caagttcgcg ggcggccgac aggatggggc cgtattcggc
     3961 ggcgtcgaag cagacgaacg gcccttcgta ccgggcctct tcggccaggt ccctggcctt
     4021 gtcgtagtgg atacccaggt cgtattcctc ctgggtgata ctgaccttga agaccggcac
     4081 gtcggggctg ccggaggcac cgatgcaggc gaccgcgact tgcatctcga tctcgctgcc
     4141 gtcggcaggt tcggtcgagg ggatgacggc tcggcccgtg ggggcgtcgt cgtccatgcc
     4201 catgcgctcg cgcatttcgt cggcgaatgc ctcgggggcc atcaggccat agcgcgccag
     4261 acgcacggga atgtcctcgg caccgaggtt gccgtcctcg atgcgctgtt gcatgaaggc
     4321 ggcgatcttg gcttcgagtg catcgcggtc cgtctccgag ggaagcaccg ccacatcgat
     4381 cgaccaggtg tccacttccg cggcggtctc gccggtcagc agaccctcgc cgatggcgcg
     4441 ttcgcacatg cgttccagcc gtgccagcaa ttccggcgcg gcctcgccgt tgagcagata
     4501 ggtcacgtcc agcgtcaagc gcgcattgac ggtttcgggc gtggcttggg tagtagcgtt
     4561 catgaaaatc tccttgggat gaaaaaaagc gcagccacgt ccacggagac cgtgggcgtg
     4621 gctgcgaggt gggaacaatg gaaagccggc ggcgcgaggc cggcgttggc gtggggatca
     4681 ggcggcgacc agttgccggg ccagtgcgac ctcgacggaa tcaccgtcct ggttgagcac
     4741 atccagcccg gattcgggta catcgccgga cgtgctttgg gacacgagga tcttcttggc
     4801 catcagcgcg aggcacgtca tctgcgaaga ggcggcgtac cccaggtaga tcaccttcac
     4861 ggcgagcttc tggccgatcc gccatgagcg gcgtgcggcc tgctgcagcg tgtacacgtt
     4921 ccaccccgac tgcaggaaaa caatcgtcgg gaattcgagc aggtccaacc ccgtcttgac
     4981 gagttcgggg ttcgtgatga gcacgtcgat gccccggtcg atctgctcgg caatccagtc
     5041 ctcgcgctgg ctggcatcca cgcttgcgcg cagtaccgcc accttgaagc cttcctgctc
     5101 cagcagcatc ttcagccggc tcgtggtgtc ccgcgtgccg gtatagaccg aatacaccag
     5161 ggttttgcga cccgcttcct tctcctcgcg gcagatgttg atcagctcgc gctccttggg
     5221 catcacctcc agctcgttga actgggccgg ggtgaatgcc agcacctcgc gcgtgcgcgg
     5281 atggcggacc gtctctgccc tgaagcagca gtcgggccac gccagcagcg tgttgaggac
     5341 cacgcccagc aaagtggtgt cccgcttgcg cagggcctcc ttgagctgtt gagtgaggct
     5401 ggaagccagc ttctgatacg cctcgccttg ctcggcgctc atcgccacgt cccggaactc
     5461 ctcgtcatac ggcggcagca cgccaccgat gtctcgcagc ttcaggaaga tggtgtacgg
     5521 caggatgcac cgcagcacgc ccttggggcc gaagccgggg gccttgaccg ttctgaccgt
     5581 gaccttgctg cctttggccg tcttgtgtgc cggaccgtcg gactcggagt agatatcccg
     5641 caagacgccg tgatccctca tgaaggccat ggcggcggcg ttcatgctgc cgttgcggcc
     5701 ggggatgtag ccgtcctcga tcatgcggcc cggaagcgcg cggaacagca ggtggaaaag
     5761 atcgtcggcg tagccgccca tcagcgtgcc cgtgagcgag accgtcttgc ggaccttgga
     5821 cgcgatgacg cccatggcct ggccttgcgc cgaaccgccc gttttgtact cgtgggcttc
     5881 gtctgcgata agcaagtcga acgtgctgcg cggcagatac cgcttcacat attcgctggg
     5941 ttggtagccg ccttcaccga aaccgaactc catgttcgcc atggaccgtt ccatgcggtg
     6001 cgcctggcgg tcggagaaga ccagcttccc gctcgcgtcc atcaggttga tgaagttgtg
     6061 caggttgtcg ccgagcatcg aggccaggaa gctgtccccg aaggtcttca tcagcttctg
     6121 cgcggtggct tcgccgatgg tgggaatacg cttcaaggca cgcagcacag ccgaggactg
     6181 gtcgctgccg gacagtgacc gcggacgcat cagcgtccac agcgcgccgc cgcagccatt
     6241 gcacttgcga cggaactctt ccgcctccag ctcctgagca gtgatcggct caccgtcgag
     6301 gttggtgacg acctctccgc agtgcgggca ggcccccacg tcaccgtgac gggtgtgctt
     6361 gcgcgcgaag gcgggcttcc agtggaaccc catcctcatg cggacacgac cgagaacgaa
     6421 aaactcctgc gccggcgaag gcacgccaag ctgctcgcgc aacttgatca gtttgagcag
     6481 cgtgtccggg ccattgagca cccagacctt tgcgccagcg acggtttcga ggatttcccg
     6541 gcgccacttg taaacaaggt gcggcgggct caggacgagc gtgcggcgat agccttcggc
     6601 atgcaggacc gcggccagcg caatgccgat cgtggtcttg ccggtgccca tctcgccgtt
     6661 gacgaaaacg gcccgctcgc cttggttgac gagcagttcc gaggccgcat gcacgacctc
     6721 ggcctgggcg ggaaacaact ggcgtttcag cccggccagc accagttgcc ggtgcgggcg
     6781 cggcgtgccg ctatagacgg gcgggttcgc ccggttgagg gattccagca gctcgtctcc
     6841 gaactcggaa acgaagtcct gaaggctcat ggacagctcg gacggttcct gttcgagcag
     6901 gtcgtccatc ggggacgagg tatcagcggc cgaggccgcg tcgatgactt gtgcgttcat
     6961 ggtgatgctc cgagaagaaa aacggggcat gcaccactcc ccaaggggcg gtgacatgcc
     7021 ccaggggtgg gaaggaaaga tcggccggat tcgggatctg gccggtgatg acgacgatca
     7081 gtactcgctg ggcagcagca ccgtggtgac gctgcgatcc cattcggtga tgacccagac
     7141 cttcaggctc gggccgacct cgtaggcaga gaacaggcgg gcctcgcccg acttcagcgc
     7201 ggcgtcgttc tggcgccggt cgctgtcgtc gaggtcgccc cagtcggcgg acagatgccg
     7261 caacaggtac ggacggggat tgagctggcc ttgctgaacc aggctggcaa cgcccgaggt
     7321 catgacgacc tggccggggt tgaatcgggg aaaagcgtgt tgggcaacgg tggacatgga
     7381 gatggctcct tcagcaaaag aggcacctcg ccccatcggg gcgagaaacc ccggtgggtg
     7441 gaaagaaaac gtgccgcggc tgcggacgct ggacgatcag ttcacggccc cttcttcggg
     7501 cagttgcacg accgtggtgc actggtcgtc actggtgatg acgagcagga acagcctcgg
     7561 tgtgatcttg aaacgcgacg tcatgggtgc gccttgctcc agcgcgacgt cgttcaactg
     7621 gcactcggcc tcactggcct cgccccagtc gccgcgcaca tggcgctgca cgtagggcaa
     7681 agggtcgatg aggctcttgc ttgccagcca atgcaccttc tcgctcagct tgagcgtgcc
     7741 cggtgcgaag agcggtggaa cctggggctt gcgcccctgg aagatggaca gcatggggat
     7801 actccttgca ggtgatgaaa aacaggcggc aagccgcccg cggggtcagt ggatggtgag
     7861 aacctgtccc agcgtggaag agctgggtgt gaggtcccaa gcccgaatca ccgggacgaa
     7921 cttgtccgtc aggattcggg tttcggccaa agagccgtcg tcgcgctcgc ggtattccac
     7981 ggcggaagac ttctgcttga acgtgtcgcc cttgacggcc agggtgtggc cggagggtga
     8041 ctgcacgaca ccggagatcg cgccggccgc gagggccagt gccaagtgcc actgggacaa
     8101 ggcgcgtgcc ggagcacgca aggtctgctg ggtcgcgccc agatgggtct cgaacgcggg
     8161 ccacaggcct ttgaggcgcc gcacttcgtc ggcgaactgt tccggctcca tgctgatccg
     8221 gtagaaatgc tccggctcgg cgggggccgt ggggaccaca tacggcagca gcgtccaatc
     8281 gggcggcagc tcgtctggct ccaactcggc caagccgatt tgcagcagcc gggcgcggga
     8341 cgctttcacg tcatcgcccg cctggtcgcg ctgccggaca cgacgcccga acaccacgac
     8401 ctgcttgaac tgcttgtcca ccgcttggaa cacggacagg tccgcgaaat ggcgtgtcag
     8461 ccagccgacc atctcctgat ccagcacgta ggacggcagg atgaagacca ggatgccgcc
     8521 gtactgcaac agaggcagcg tgcgctggta gaagagcttt tccagacggg cgcggccctt
     8581 gccttcgtag cccaggttgc cgctggtgtc ccttgcgaga tcgccatagg gcgggttgag
     8641 ccacaggagg ccgaaggcct gccgcgagat catggtgtcc atcaggtcac cgtggatgca
     8701 gtgatcgacc agcagccggg cgtgggtggc tcgttcctga tcgaactcga cggcataggc
     8761 gctggccagc tcaggcccga gggcatgggc ggcttcggcg atggcaacgc cttcgccagc
     8821 gcagggatca caaatgcaca tgggcccatc gctgggcgcg agcgcgctga gggttctttc
     8881 gagggtggct tcgtcggtcg ggtagtaccc gttcttggcg aaattccgag caagcctggg
     8941 gaacatgagt gccatgggaa gtctcctgtc tggcggggat gaagaacgga agcacgccaa
     9001 ggcgtgcctc cgcggtgggg tcaaaccgca gccggctccg gcgtccaaat cctggccgga
     9061 tagggatagg cggtgagcac gccctggcgg atgaggtcgc ccagcgcatt ggtgagcgcg
     9121 ggcacgtcga tggcgagccg gaacccttgc agcggcccga gggccatgcg cagggagcac
     9181 agcatttcgc gctcgcgcag cagcgccagg actggcgatt gccagtggtc cagcagcggc
     9241 agcgggcacg tgtccttgac caggtgccac aggcgtgacg tgggatcggc cgcggagcgc
     9301 ggcagcagcg ccaaggcgct ggccgaactc ttgtcgggcc ggatgcagcg ccggtcgaac
     9361 agccagagat tgaccatcga gccgaacagc gtgcggcgat agctgcgggt caggcgcttt
     9421 tcaaggcgct
//
LOCUS       DATCUN010000236         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_160774, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000236 DATCUN010000000
VERSION     DATCUN010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160774"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..2332)
                     /gene="uvrD"
                     /locus_tag="VIO83_09060"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(155..2332)
                     /gene="uvrD"
                     /locus_tag="VIO83_09060"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEY8331460.1"
                     /translation="MREDLSFLLSSLNDAQRQAVAAPLGRQLVLAGAGSGKTRVLVHR
                     IAWLIEVEGAGAHSILSVTFTNKAAAEMRVRIEQLLGVSPAGMWVGTFHGLAHRLLRA
                     HWQEAGLAENFQILDSDDQQRLVKRVIRELGLDEQRWPARQAQWFINGQKDEGIRPQH
                     IQASGDLYVATMLQIYSAYEAACKRAGAIDFAELLLRALDLWRDHPGLLEHYQRRFRH
                     ILVDEFQDTNAVQYAWLRLLGQGGASLMVVGDDDQSIYGWRGARIENIQQFGRDFADA
                     QTIRLEQNYRSTARILKAANALIAHNQGRLGKELWTEGHEGDPLALYAAFNEYDEARY
                     VVESIMEAVRKDGLKRSEIAILYRSNAQSRVLEESLLREKIPYRIYGGQRFFERAEIK
                     NAMAYLRLVREREDDAALERVLNLPPRGIGEKTVETLREIARSENLSLWRALHQAVGN
                     KLVPGRAAGALNAFVELIDSLALRVEAMPLHLQTQTVIEQSGLIPFHLAEKGEKGQTR
                     IDNLEELVSAARSFETSEEELSPLAAFIDHAALEAGDTQADVDEDSVQLMTLHSAKGL
                     EFPLVFLVGMEEGLFPHKMSMEEPGRLEEERRLAYVGLTRAMQRLVLTYAETRRLYGS
                     ETYNKVSRFVREIPPGLIHEVRLANTVSRPYTGNRSVASPFAGASVPETAFALGQQVR
                     HSLFGEGVILNYEGSGAQARVQVNFASEGTKWLMLSYAKLEAL"
     gene            2468..>2714
                     /locus_tag="VIO83_09065"
     CDS             2468..>2714
                     /locus_tag="VIO83_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF-domain containing protein"
                     /protein_id="HEY8331461.1"
                     /translation="MTFLFSSDVAPPVIEPDAVRQHYAAQIEVAVTRQLYRGSRLPTL
                     LMLLGSLSGVGLLWGERGHGLLAGLLAWLSLLAGLRLW"
BASE COUNT          378 a          905 c          981 g          450 t
ORIGIN      
        1 ctgcggcgcg gccggctccg gccggcagcc ggccagcccc agggcggcca gcgcggcggt
       61 ggcaccgaac aggtggcgac gcttcatcga ctctctccct ttcgcgatga ggccgcaggc
      121 ggccgcgagg gtggttcggg ccgcggtcgg cgggctacag cgcttccagc ttggcgtagg
      181 acagcatcag ccacttggtg ccttcgctgg cgaagttcac ctgtacccgc gcctgggcgc
      241 cggagccttc gtagttgagg atcacccctt cgccgaacag gctgtggcgc acctgctggc
      301 cgagggcgaa ggcggtttcc ggcacgctgg cgccggcgaa cgggctggcc acgctgcggt
      361 tgccggtata cggacggctg accgtgttgg ccaggcgcac ctcgtggatc agtcccggcg
      421 ggatctcgcg gacgaaacgc gacaccttgt tgtaggtctc gctgccgtac agacggcggg
      481 tctcggcgta ggtcagcacc aggcgctgca tggcgcgggt caggccgacg taggcgaggc
      541 ggcgctcctc ctcgagacgg ccgggctcct ccatgctcat cttgtgcggg aacaggcctt
      601 cctccatgcc gaccagaaac accagcggga actccaggcc cttggcgctg tgcagggtca
      661 tcagctgcac gctgtcctcg tcgacgtcgg cctgggtgtc gccggcttcc aaggcggcgt
      721 ggtcgatgaa cgcggccagc ggcgacagct cttcctcgct ggtctcgaac gagcgcgcgg
      781 cgctgaccag ttcctcaagg ttgtcgatgc gggtctggcc cttctcgccc ttttcggcga
      841 ggtggaaggg gatcagcccg ctctgctcga tcaccgtctg ggtctgcagg tgcagcggca
      901 tcgcctcgac gcgcagggcg aggctgtcga tcagctcgac gaaggcgttc agcgcaccgg
      961 cggcgcggcc ggggaccagc ttgttgccga ccgcctggtg cagcgcgcgc cacagcgaca
     1021 ggttctcgct gcgggcgatc tcgcgcaggg tttccacggt cttctcgccg atgccgcgtg
     1081 gcggcaggtt gagcacccgc tccagcgccg cgtcgtcctc gcgctcgcgc accaggcgca
     1141 ggtaggccat ggcgttcttg atctcggcgc gctcgaagaa gcgctggccg ccgtagatgc
     1201 ggtagggaat cttctcgcgc agcaaggact cttcgagcac ccgcgactgg gcgttggagc
     1261 ggtagaggat ggcgatctcg ctgcgcttga ggccgtcctt gcgcaccgcc tccatgatgc
     1321 tctcgaccac gtagcgcgcc tcgtcgtact cgttgaaggc ggcgtacagg gccagcggat
     1381 cgccctcgtg gccctcggtc cacagctcct tgccgaggcg gccctggttg tgggcgatca
     1441 gcgcgttggc ggccttgagg atgcgcgcgg tggagcggta gttctgctcc aggcggatgg
     1501 tctgggcgtc ggcgaagtcg cggccgaact gctggatgtt ctcgatgcgc gcgccgcgcc
     1561 agccgtagat cgactggtcg tcgtcgccga ccaccatcag gctggcgccg ccctggccga
     1621 gcaggcgcag ccaggcgtac tgcacggcgt tggtgtcctg gaactcgtcg accaggatgt
     1681 gccggaagcg ccgctggtag tgttcgagca ggccggggtg gtcgcgccac aggtccagcg
     1741 cgcgcagcag cagctcggcg aagtcgatcg cgccggcgcg cttgcaggcg gcctcgtagg
     1801 cgctgtagat ctgcagcatg gtcgccacgt acaggtcgcc ggacgcctgg atgtgctgcg
     1861 ggcggatgcc ctcgtccttc tggccgttga tgaaccactg cgcctggcgc gccggccagc
     1921 gctgctcgtc gagacccagc tcgcggatca cccgcttgac caggcgctgc tggtcgtcgg
     1981 agtcgaggat ctggaagttc tccgccagcc ccgcctcctg ccagtgggca cgcagcagac
     2041 ggtgggccag gccgtggaag gtgcccaccc acatgccggc cgggctgacg ccgagcagct
     2101 gctcgatgcg cacgcgcatc tcggcggcgg ccttgttggt gaaggtgacc gagaggatcg
     2161 agtgggcgcc ggcgccttcg acctcgatca gccaggcgat gcgatgcacc agcacgcggg
     2221 tcttgccgga gccggcgccg gccagcacca gctggcggcc gagcggcgcc gcgacggcct
     2281 ggcgttgggc atcgttgagg gaactaagca gaaaggagag gtcttcgcgc atcgcggcat
     2341 tctagcggtc cgcgcgaaga cgcggcaaac tccgtgcggt cgggagtacc ggcccagtcc
     2401 tgcagggagg cgaccaccgc atgtccggga cgacaatcaa cacagaacaa caagaaaagc
     2461 cgcacacatg acctttctgt tttcgtcgga tgtcgcgccg ccggtgatcg agccggatgc
     2521 ggtgcgccag cactatgccg cgcagatcga ggtggccgtg acccgccagc tgtaccgcgg
     2581 ctcgcgcctg cccacgctgc tgatgctgct gggcagcctg agcggcgtcg gcctgctctg
     2641 gggcgagcgg ggccacggcc tgctggcggg cctgctggcc tggctgagcc tgctggccgg
     2701 cctgcgcctg tggc
//
LOCUS       DATCUN010000237         3384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_235812, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000237 DATCUN010000000
VERSION     DATCUN010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235812"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VIO83_09070"
     CDS             <1..1212
                     /locus_tag="VIO83_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8331462.1"
                     /translation="GRLVLIRLLVLAAQAGSVGFAWWSDRLPLPWTELGITLGVSGLL
                     SIFTALRLRASWPVTELEYAVQLGCDLVIHSVLLYYTGGSSNPFVSYYLVPLTIAAAT
                     LPWLHTLTLAGLALASYTLLLVWSHPLDLPAGPQESLLVYGMWLSFALAAALVTFFVA
                     KMAEELRRQEQLRAQRREEGMRDQQLLAVATQAAGAAHELGTPLSTMSVLLKELRQEN
                     RDQPALQEDLALLQEQVKLCKETLQQLVRAAEAERRQSVSEQTAREWLEAVLQRWHLM
                     RPEATYRYQCVGVGTPPRLMPPTDLSQALLNLLNNAADACPDQLDIRLDWDAREICLS
                     IRDHGVGVPLAIAEQLGKPFFTTKGKGFGLGLFLSQASVTRVGGTVKLYNHEEGGTLT
                     ELRLPRNYGVA"
     gene            1241..1801
                     /locus_tag="VIO83_09075"
     CDS             1241..1801
                     /locus_tag="VIO83_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8331463.1"
                     /translation="MSEEALFDAEEHPHLLLVDDDETFTRVLARAMSRRGLRVSVANS
                     AEEGLLLAERDIPDYAVLDLKMDGDSGLVLLPKLLELDAEMRVVILTGYSSIATAVEA
                     IKRGACNYLCKPADADDVLAALLSEHADLDSLVPDNPMSVDRLQWEHIQRVLTEHDGN
                     ISATARALGMHRRTLQRKLQKRPVRR"
     gene            2039..2980
                     /locus_tag="VIO83_09080"
     CDS             2039..2980
                     /locus_tag="VIO83_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY8331464.1"
                     /translation="MLALFHATLGVTAPVFAMLFLGVLLKRLGWIDEAFINTASALVF
                     KATLPTLLFLGILRADLSTALQPALLGYFVVATLGSFLLAWGWAVWRVPYAERGVFTQ
                     GAFRGNCGIVGLALATSLYGDYGLSLGGVLAGVVILSYNVLSVIVLALYSPGVKSDPW
                     SLVKGVLSNPLIIGVLAALPLAGFEIALPHWLLTSGGYFAQMTLPLALICIGGTLSLA
                     SIREGSALALGSSLFKLVWLPLLCTLGAWLCGFSGRELGILFLYFASPTAAASFVMAR
                     AVGANHQLAASIIVLTTLGAVLSINLGLFVLTGLGWI"
     gene            complement(3039..>3384)
                     /locus_tag="VIO83_09085"
     CDS             complement(3039..>3384)
                     /locus_tag="VIO83_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEY8331465.1"
                     /translation="VGSNIFNLLCVLGLASLVSPTPLSVSANAIAFDYPVMIAVAMAC
                     LPVFFAGYCIRRWEGALFFAYYLIYTAWLVMHSTDAPGLAMFSQAMTWFVLPLTLTTL
                     LVIGGRAWQQQR"
BASE COUNT          482 a         1136 c         1161 g          605 t
ORIGIN      
        1 ggccggctgg tccttatccg tctgctcgtg ctggccgccc aggccggctc ggtggggttc
       61 gcctggtggt ccgaccgcct gccactgccg tggaccgaac tgggcatcac cctcggcgtc
      121 tccgggctgc tcagcatctt cacggcgctg cgcctgcgcg cctcctggcc ggtcaccgag
      181 ctggaatatg cggtacagct gggctgcgac ttggtgatcc acagcgtgct gctgtactac
      241 accggcggct cgagcaatcc cttcgtctcc tactatctgg tgccgctgac catcgccgcg
      301 gcgaccctgc cctggctgca taccctgacc ctggcgggtc tggcgctggc cagctacacc
      361 ctgctgctgg tctggtcgca tccgctggat ctgccggccg ggccgcagga aagcctgctg
      421 gtctatggca tgtggctgag ctttgccctg gcggcggcgc tggtgacctt cttcgtcgcc
      481 aagatggccg aggagctgcg tcgccaggag cagttgcgtg cccagcgccg cgaggagggt
      541 atgcgcgacc agcagctgct ggccgtggcc acccaggccg ctggcgcggc ccatgagctg
      601 ggcacgccgc tgtcgaccat gagtgtgctg ctcaaggagc tgcgtcagga aaaccgcgac
      661 caaccggcgc tgcaggagga tctggccctg ctgcaggagc aggtcaagct gtgcaaggag
      721 accctgcagc agctggtgcg cgcggccgag gccgagcgcc gccagtcggt cagcgagcag
      781 accgcccgcg aatggctgga agcggtgctg cagcgctggc acctgatgcg tccggaggcg
      841 acctatcgct accagtgcgt gggggtcggc acgccgccgc ggctgatgcc gccgaccgac
      901 ctcagccagg cgctgctcaa cctgctgaac aatgccgcgg atgcctgccc ggaccagctc
      961 gacatccgcc tggactggga tgcgcgggag atctgcctga gcatccgcga ccatggcgtc
     1021 ggtgtgccgc tggcgatcgc cgagcagctc ggcaagccgt tcttcaccac caagggcaag
     1081 ggcttcggcc tgggcctgtt cctcagtcag gccagcgtga cccgcgtcgg tggtacagtg
     1141 aagctctaca accacgaaga gggcggcacg ctgaccgagt tgcgactgcc gcgcaattac
     1201 ggcgtcgcct gagcgccggt cctgccttgt gaggaatgaa atgagcgaag aagccctgtt
     1261 cgatgcggaa gagcacccgc atctgctgct ggtggacgac gacgaaacct ttacccgcgt
     1321 gctggcgcgc gccatgagcc ggcgcggcct gcgcgtgtcg gtggccaact cggctgagga
     1381 agggttgctg ctggccgagc gcgacatccc cgactatgcg gtgctggacc tgaagatgga
     1441 cggcgactcc ggcctggtcc tgctgcccaa gctgctggag ctggacgccg agatgcgcgt
     1501 ggtgattctc accggctact ccagcatcgc caccgccgtg gaggcgatca agcgcggtgc
     1561 ctgcaactac ctgtgcaagc cggcggacgc cgacgacgtg ctggcggcgc tgctctccga
     1621 gcatgccgac ctcgacagcc tggtgccgga caatccgatg tcggtggatc gcctgcagtg
     1681 ggagcacatc cagcgcgtgc tgaccgagca cgacggcaac atctccgcca ccgctcgtgc
     1741 cctgggcatg caccggcgca ccctgcagcg caaactgcag aagcgcccgg tgcgccgctg
     1801 agccaagcga ggtctgggcg ttgatggggc gcagttgcgg gcggcacccg gccgcctcgc
     1861 gttccgcagc cggcctgtgg gcgatgcttt tacgccgcta cggctcgcca gccaacgggc
     1921 tcctgcaggc gctgcgcttt agccgctagc cgaagctgcg gctgctggcg cctggccaat
     1981 caattcgtcc gccagtgcct gttaagatgg cgccctctcg cgccatccgg atgcccccat
     2041 gcttgccctg ttccacgcaa ccctcggcgt caccgcgcca gtgttcgcca tgctgttcct
     2101 cggcgtgctg ctcaagcgcc ttggctggat cgatgaagcc ttcatcaata ccgcctcggc
     2161 gctggtgttc aaggccaccc tgccgaccct gctgtttctc ggcatcctcc gcgccgacct
     2221 gtccacggcc ctgcagcccg cgttgctcgg ctatttcgtc gtggcgaccc tgggcagttt
     2281 cctgctggcc tggggctggg cggtgtggcg cgtgccgtat gccgaacgcg gggtgttcac
     2341 ccagggcgcc tttcgcggca actgcgggat cgtcggcctg gccctggcca ccagcctcta
     2401 cggtgattac ggcctgtcgc tcggcggtgt gctggcgggg gtggtgatcc tctcctacaa
     2461 cgtgctgtcg gtgatcgtgc tggcgctcta cagtccgggc gtcaagtccg acccctggag
     2521 cctggtcaag ggcgtgttga gcaatccgct gatcatcggc gtgctggcgg cgctgccgct
     2581 ggccgggttc gagatcgccc tgccgcactg gctgctgacc tccggcggct acttcgcgca
     2641 gatgaccctg ccgctggcgc tgatctgcat cggcggcacc ctgtcgttgg cctcgattcg
     2701 cgagggcagt gcgctcgcgc tgggctccag cctgttcaag ctggtctggc tgccgctgct
     2761 gtgtaccctc ggcgcctggc tgtgcggttt cagcgggcgc gagctgggca tcctgttcct
     2821 ctacttcgcc agcccgacgg ccgcggcgag cttcgtcatg gcacgtgcgg tgggcgccaa
     2881 tcaccagctg gcggcgtcga tcatcgtgct gaccacgctg ggcgcggtgt tgagcatcaa
     2941 cctcggcctg tttgtattga ccgggctggg ctggatctga ggacggttgt gccgttgggg
     3001 tgcgcatggc gcacccggct ggcaaggggc ggcgctcact agcgctgctg ttgccatgcc
     3061 ctcccgccga tcaccagcag ggtggtgagg gtcagcggta ggacgaacca ggtcatcgcc
     3121 tggctgaaca tcgccaggcc cggtgcgtcg gtgctgtgca tcaccagcca ggcggtgtag
     3181 atcaggtagt aggcgaagaa cagcgcgcct tcccagcggc ggatgcagta gccggcgaag
     3241 aacaccggca ggcaggccat ggccacggcg atcatcaccg ggtagtcgaa ggcgatggcg
     3301 ttggccgaca cgctcagcgg cgttggcgac accagcgagg ccagaccgag cacgcagagc
     3361 aggttgaaga tgttgctgcc gacc
//
LOCUS       DATCUN010000238         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_273681, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000238 DATCUN010000000
VERSION     DATCUN010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273681"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VIO83_09090"
     CDS             complement(<1..1290)
                     /locus_tag="VIO83_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008047633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HEY8331466.1"
                     /translation="MNKHGIVGTLSRTPLDKLALAIALATVASTALAEDRFKYDGYLR
                     EELSWNMKDWEDTPGYDDKGKMSMARSTARLNLEWQATDALSVVAKLRATREVKTNFL
                     EQLEEMGAYNYREADGQGDIMDLYNDEEIRELYVDFPVGERLQFRLGKQQIAWGETDF
                     FAANDLVHGFDYTWRSFLEPANEELRKANIIAKMNIDVPEWDGGLEVFVRPGWDDKND
                     IGTELDIYGGRWSSQPYAGVDFRNIDPYNFENDEGDYRDVTGGIRWSGLYGDTNYSVS
                     YLKTYYPSPILNASNNLALFGLPGVPSGAETEGTTQTRGPAGEIIYPMVDIFGFTASR
                     YSGWADAVFSTEMAYIKDAPYQILQSPPTLISQAVAPGFDGYATKDVVALMLRMDKNI
                     AATQDWLGTEKPMFFSVQLFDKWVQNYDRDDALLYSVG"
     gene            complement(1353..>1677)
                     /locus_tag="VIO83_09095"
     CDS             complement(1353..>1677)
                     /locus_tag="VIO83_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY8331467.1"
                     /translation="YIADRVKEEIAIGSSPEHAIRQALHSSGMGVIVTASVLILATLL
                     WWVSSLRFQAEMGLLMAIWLSVSAFSALFVMPALIYVTRPRFIFGERAPEPAASAQPA
                     PFQPA"
BASE COUNT          260 a          510 c          587 g          320 t
ORIGIN      
        1 gccgacgctg tagagcagcg cgtcgtccct gtcgtagttc tgcacccact tgtcgaacag
       61 ctgcaccgag aagaacatcg gcttctcggt acccagccag tcctgggtgg cggcgatgtt
      121 cttgtccatg cgcagcatca gggcgaccac gtccttggtg gcgtagccgt cgaagccggg
      181 ggccaccgcc tggctgatca gggtcggcgg cgactgcagg atctggtagg gcgcgtcctt
      241 gatgtaggcc atctcggtgc tgaacaccgc gtccgcccac ccggagtagc ggctggcggt
      301 gaaaccgaag atgtcgacca tcgggtagat gatctcgccg gccggaccgc gggtctgcgt
      361 ggtgccctcg gtttccgcgc cggacggcac gcccggcagg ccgaacaggg cgaggttgtt
      421 ggaggcgttg aggatcggcg acgggtagta ggtcttcagg taggagaccg agtagttggt
      481 gtcgccatac aggccgctcc agcggatgcc gccggtgacg tcgcggtagt cgccctcgtc
      541 gttctcgaag ttgtacggat cgatgttgcg gaagtcgacg ccggcgtacg gctggctcga
      601 ccagcgcccg ccgtagatgt ccagctcggt gccgatgtcg ttcttgtcgt cccagccggg
      661 acgcacgaac acctccaggc cgccgtccca ctccggcacg tcgatgttca tcttggcgat
      721 gatgttggcc ttgcgcagtt cctcgttggc cggctcgagg aagctgcgcc aggtgtagtc
      781 gaagccgtgc accaggtcgt tggcggcgaa gaagtcggtc tcgccccagg cgatctgctg
      841 cttgccgagg cggaactgca ggcgctcgcc gaccgggaag tcgacgtaca gctcgcggat
      901 ctcctcgtcg ttgtagagat ccatgatgtc gccctggcca tcagcctcgc ggtagttgta
      961 ggcgcccatc tcctcgagct gctcgaggaa gttggtcttc acctcgcggg tggcgcgcag
     1021 cttggctacc accgacagcg catcggtggc ctgccactcc aggttgaggc gcgcggtgct
     1081 gcgcgccatc gacatcttgc ccttgtcgtc gtagccgggg gtgtcttccc agtctttcat
     1141 gttccaggac agctcctcgc gcaggtagcc gtcgtacttg aagcggtctt cggcaagggc
     1201 ggtcgaggca accgttgcca aggcgatcgc cagcgcgagc ttgtccagag gggtgcgcga
     1261 gagcgtaccc acgatgccgt gcttgttcat gtgttatccc cagagtggtt ggcagcgagg
     1321 aaccgatggt ttcttgttgt tgttatcccg tctcaggccg gctggaacgg ggcgggttgg
     1381 gcactggccg ccggctccgg cgcgcgctcg ccgaagatga agcgcgggcg ggtgacgtag
     1441 atcagcgcgg gcatgacgaa cagggcggag aacgccgaca cgctcagcca gatggccatc
     1501 agcaggccca tctcggcctg gaagcgcagc gaggagaccc accacagcag ggtggcgagg
     1561 atcagcacgc tggcggtgac gatgaccccc atgcccgagc tgtgcagggc ctggcggatg
     1621 gcgtgttcgg ggctgctgcc gatggcgatc tcttccttga cgcggtcggc gatgtag
//
LOCUS       DATCUN010000239        24987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_160814, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000239 DATCUN010000000
VERSION     DATCUN010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 24987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24987
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160814"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VIO83_09100"
     CDS             <1..873
                     /locus_tag="VIO83_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEY8331468.1"
                     /translation="LRREIVSTQIANDLVNHMGITFVQRLSEATGMSAANVAGAYVIV
                     RDIFHLPHWFRQIEALDYKVPAELQLRLMDELVRLGRRATRWFLRNRRSELDAARDVA
                     HFGPRVAALGLKLDGLLEGSTRDLWQSRYQRYTAAGVPELLARMVAGTSHLYTLLPIL
                     EAADETGQPPIKVASTYFAVGGALELPWYLQQLTSLPVESTWQALAREGFRDDLDSQQ
                     RAITVSILQMESGAQQPAACVMEWLEQRGYHVDRWRGMLAELRATNTPDYAMYVVASR
                     ELQDLAQAPVRHGE"
     gene            complement(918..1835)
                     /locus_tag="VIO83_09105"
     CDS             complement(918..1835)
                     /locus_tag="VIO83_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6685 family protein"
                     /protein_id="HEY8331469.1"
                     /translation="MTSLPESMPSLSTHITRLAQRLGLRAQPGHQTLERASRLNLPFA
                     PLYEPEPSIFWNDGPCLQRLLELPRGALSGPVQEDKARAHAALSRLIQKDSQSLASID
                     LRQIDGLGGQSPDGIAYPSIEAFAATEACRRTRIISYKDFLRTLGIALPGHAQDEPIE
                     LRQAGWLGERLYWAGEHHVEAFASAVAYARLRGLELLMPAELVVYRLDRAGLRALDEQ
                     YHAIAMPAQAWSDAEFMALLLDTQVPYARLTVLRNASNSELLLLEKHHPTASAIGEGL
                     KRAGAPDMVRYLRNLPQITRFKLGQPQDS"
     gene            2002..2223
                     /locus_tag="VIO83_09110"
     CDS             2002..2223
                     /locus_tag="VIO83_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2835 domain-containing protein"
                     /protein_id="HEY8331470.1"
                     /translation="MPSLVLDIALPAERLLAAYQGHANRVLLRSRDGRRISLPVHHLR
                     PFVTREGVFGSFELEFAQQGQLIGLHRLR"
     gene            2314..3345
                     /locus_tag="VIO83_09115"
     CDS             2314..3345
                     /locus_tag="VIO83_09115"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY8331471.1"
                     /translation="MYNLARQMLFKLSPETSHELSLDLIGAGGRLGINRLLSKSPASL
                     PVRVMGIDFPNPVGLAAGLDKNGEAICGLSQLGFGFIEVGTVTPRPQPGNPKPRIFRL
                     PEAEAIINRMGFNNQGVDALLARVDAARFKGVLGINIGKNFDTPVERADEDYLLCLDK
                     VYHHASYVTVNVSSPNTPGLRSLQFGDSLKRLLEALRLRREDLEIEHGKRVPLAIKIA
                     PDMTDEETVLVAEAVLQAGMDAIIATNTTLSREGVAGLAHADEAGGLSGAPVREKSTH
                     TVRVLAQTLSGRLPIIAVGGITEGRHAAEKIAAGASLVQLYSGFIYKGPALIREAVDA
                     IAELRAKAQ"
     gene            complement(3465..3680)
                     /locus_tag="VIO83_09120"
     CDS             complement(3465..3680)
                     /locus_tag="VIO83_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HEY8331472.1"
                     /translation="MRRLKRDPMERAFLRGYQHGIHGKSRDLCPFTHPTVRQAWINGW
                     REGRGDNWDGLTGTAGLHRLNELHAVG"
     gene            4038..6224
                     /gene="rlmKL"
                     /locus_tag="VIO83_09125"
     CDS             4038..6224
                     /gene="rlmKL"
                     /locus_tag="VIO83_09125"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HEY8331473.1"
                     /translation="MTDRHELILTCPKGLEGLLLEEARQLGLEDAREQTAAIRGFGAL
                     EVAYRLCLWSRLANRVLLVISRFPTTTAETLYEGVRSVDWAEHLEPGGSLAVEFSGRG
                     SGIDNTHFGALKVKDGIVDRLRTASGERPTVDKLNPDLRVHLRLDKGEAVLSLDLSGH
                     SLHQRGYRLQQGAAPLKENLAAAVLIRAGWPRIAAEGGALTDPMCGVGTFLIEGAMIA
                     ADIAPNLKRERWGFSAWLGHVPALWHRLHDEAEARAAAGLARPPLWIRGYEADPRLIQ
                     PGRNNIERAGLGSWIRIYQGDVATFEPRPDQNQKGLVICNPPYGERLGDEASLLYLYQ
                     NLGERLRQACLGWEAGIFTAAPDLGKRMGIRSHKQYAFWNGALPCKLLLIKVELDQFV
                     TGNRTAAPQANAAPAPVEVARLSEGGQMFANRLQKNLRQLGKWARREGIECYRLYDAD
                     MPEYAVAVDLYRDWVHVQEYAPPRSIDPDKAQARLLDALAAIPQALDMDPSWVVIKRR
                     ERQAGKKQYERQDQQGRFMEVSEGGVKLLVNLTDYLDTGLFLDHRPLRLRIQREAAGK
                     RFLNLYCYTATATVHAAKGGARSTTSVDLSKTYLDWARRNLALNGFSDKHKLEQGDVM
                     EWLEADRGEYELIFIDPPTFSNSKRMEGVFDIQRDHVRLLDLAMARLARGGVLYFSNN
                     FRKFQLDPSLEERYAVEEISAQTLDPDFVRNPKIHRAWCLSARQGV"
     gene            complement(6351..7811)
                     /gene="dacB"
                     /locus_tag="VIO83_09130"
     CDS             complement(6351..7811)
                     /gene="dacB"
                     /locus_tag="VIO83_09130"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY8331474.1"
                     /translation="MVRPLRLLGLAGLFLPMILPLPAAEAALPPRVEQALKASKINSD
                     ALSLALIPLDGPGTASYFNADAPVNPASTMKLVTTYAALELLGPTYQWKTGFYTDGPL
                     RNGVLQGNLYLKGGGDPKLTMERLWLLLRDLRNAGVQQVQGDLVLERNHFRPALHTSF
                     NDDGGDSSKPYLVEPDPLMVNLKTLRLIVRGEARGAAVQVEPPIPYITIDNRVRVTPP
                     AACPSWPQVNYNVQPQQGSGLTLVATGQIPEGCSGQRYMSLLDHPTFSAGAVRALWSE
                     LGGSIQGQDRLAATPGNARLLAQAWSPDVVEIIRDINKYSNNTMARQLFLSIGARHRL
                     PSDSDDAAAAYRVIDQWLTRKGIRAPHLVLENGSGLSRNERISAREMAAMLHAAWNSP
                     YAAEYISSLPLAAMDGTLRKRMRNTPLAGNAHLKTGTLNNVRALAGFSRDAQGHTWAV
                     VAILNHPRPWGASAILDQILQELYRQPGALSANAGR"
     gene            8013..8351
                     /locus_tag="VIO83_09135"
     CDS             8013..8351
                     /locus_tag="VIO83_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding protein YggL"
                     /protein_id="HEY8331475.1"
                     /translation="MATNRSRRLRKKLCVDEFQELGFELLLNYREDLDEVAVDAFLGQ
                     FLKEAISDNDMIFIGCDEFGMVCLGKRGSVNEEQRAKVDAWLKGRSELESYTLGKLAD
                     AWYPEKPINA"
     gene            complement(8422..9753)
                     /locus_tag="VIO83_09140"
     CDS             complement(8422..9753)
                     /locus_tag="VIO83_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEY8331476.1"
                     /translation="MDWHTLLTRERFGKPVQSSEELGRSAFHKDHDRIIFSGAFRRLG
                     RKTQVHPVSSNDHIHTRLTHSLEVACVGRSLGMRVGEMLREALPRWCDPGDLGVIVQS
                     ACLAHDIGNPPFGHSGEDAIRHWFQQADERGWLDALSEAERADFLGFEGNAQGFRVLT
                     QLEYHQFDGGTRLTYATLGSYLKYPWTARHADAQGYKKHKFGCYQSELPLLEQIARKL
                     GLPQLENERWARHPLVYLMEAADDICYGLIDLEDGLEMDLLDYPEVEALLLDLVGDDL
                     PETYRQLGPRDSRRRKLAILRGKAIEHLTNAAARAFVAQHDALLAGSLEGDLVEHMHG
                     PAKQCVLRAKALAREKIFQDKRKTLHEIGAYTTLEILLNAFCGAVLEQHGGRTPSFKN
                     RRILDLLGNNAPDPHWSLYKAYMRVIDFIAGMTDSYATEMAREMTGRSSPV"
     gene            10329..11537
                     /locus_tag="VIO83_09145"
     CDS             10329..11537
                     /locus_tag="VIO83_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY8331477.1"
                     /translation="MENLHSAVESLIHGSNTLFILLGAVMVLAMHAGFAFLEVGTVRQ
                     KNQVNALSKILADFAVSSLAYFFIGYWVAYGVNFFGPAGAMTADNGYALVKFFFLLTF
                     AAAIPAIISGGIAERSKFGPQLCATVLIVSFVYPLFEGMVWNGNFGFQAWLLERFGAS
                     FHDFAGSVVVHAFGGWLALGAVLMLGTRNGRYRDGRLVAFAPSNIPFLALGSWILIVG
                     WFGFNVMSAQTLQGVSGLVAVNSLLAMVGGTLAALLVGRNDPGFLHNGPLAGLVAVCA
                     GSDLMHPVGALFTGVVAGAMFVWAFTATQNRWKIDDVLGVWPLHGLCGLWGGIACGIF
                     GQQALGGVGGVSLVSQLLGTGLGVALALAGGLLVYGVLKSTLGIRLSAEEEYYGADLS
                     IHKIGATSHD"
     gene            complement(11601..12869)
                     /locus_tag="VIO83_09150"
     CDS             complement(11601..12869)
                     /locus_tag="VIO83_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015802 - basic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family porin"
                     /protein_id="HEY8331478.1"
                     /translation="MSLTPLARAVALATLGAGMILPSLAQADFVKDSKASLELKNFYF
                     NRDFRSNTSGNHKAEEWAQGFILRMESGYTEGTIGMGVDAIGTLGLQLDSGRGTSGTG
                     LLLRDRETGQPQDSYGDLGVTAKFKASNSVLKVGTLIPKLPSVSASESRLLPQSFQGA
                     HLNSQEIAGLTLDIGQLNRINNRDSQDHQAMVITSGGRRNIRFAPGTDSDAMTFAGAS
                     YKWNSQLTTAYHYGNLDEFYRQHIFNVVHVLPIADGQSFKSDLRYARSLDDESSNVDN
                     KAMGALFTYTLQGHSFGLGYQSMSGDTGYAYVGDSTDPFLVNYVQISDFANRDEKSWQ
                     ARYDFNFATVGIPGLTFMTRYLSGDNVELGGTRAEGKEWERDSELKYVFQEGTLKNLG
                     IHWRNATVRSNFASDLDENRLIVSYTLPLL"
     gene            complement(13084..15045)
                     /locus_tag="VIO83_09155"
     CDS             complement(13084..15045)
                     /locus_tag="VIO83_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020299718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY8331479.1"
                     /translation="MFVNLLIILAASLLVIALFRRLKLPPLLGYMSVGLALGPGSLGW
                     LEQHEDMPLLAEFGVVFLLFSLGLEFSLPRMLALRRVVFGLGSLQVIACTLPLAGLLH
                     LAGLTLPAALLLGGGLALSSTAIVTKELGSLGEVFTPHGQNAIGVLLFQDLVAVLLLT
                     MIPVIAGGAEQTWYWALPITLGKAALLFTALLVASRWLLPRLFHEVAQANAAELFVLL
                     ALVIVLLTAWLTHLLGLSMALGAFLAGMLLGESHYRHQIEADIRPFRDILLGLFFVSV
                     GMLIDLSLFASHGLLILGMAVLLLLLKGSLVALLVRLRGDSSESAWRSGLALAQGGEF
                     CFALVSQAQQARLVSIETGGLILAATFCSMAVTPLLLRAAPHLVARLGQRPHAEGSAR
                     EIAAACTELDQHVLICGFGRVGQSIGRFLRSEGIPFVALDNDPVRVQEAAAGEHSVHF
                     GDARRGELLTALGVARARLLVIAADNPEHALATVKEARKFSETLPILVRTRDDSHLAS
                     LQAAGASEVVPELLESSLMLASHALVMLGLPERRVQARIDEVRRSRYHMLHGFYHGAS
                     ANLLDQQGRPRTLLHAVNLSADAYACGRTLAELDLPAMGVALQAVRRDGGELALPGNH
                     ALCEGDALLLAGPLDAIESAEAWLLAGIR"
     gene            15199..15459
                     /locus_tag="VIO83_09160"
     CDS             15199..15459
                     /locus_tag="VIO83_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY8331480.1"
                     /translation="MLPDCLLFGTLGCHLCEQAEEQLMPFVGLGLQVELVDIAEREEW
                     VEAYGLRIPVLRRVDTAAELGWPFDGQSVAEFLGLPPGPAGG"
     gene            15605..16675
                     /locus_tag="VIO83_09165"
     CDS             15605..16675
                     /locus_tag="VIO83_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8331481.1"
                     /translation="MPEKVSGPQAFCLVQLEMIMSLVKPAALFVCVALAGCASKSDSG
                     NASAKDGWRSFGGKETAARAEQGKSAEAAKQVEDKAELAPAKPVEKKPQTAAKTGQKP
                     APVASRVESKKAAPVAAKLEQKKAAQKPEPTAVSKVEPKAEPAAAQPADDNALKATQA
                     EVKKTEVKKAGSSWWRFGGKDKAVDTQAQLQSKKSERQPVSKAWLDDNEKRLKAAVAG
                     SKFEVERRGDLLVLVAPADTSFNRDRPHMLLPVTLGPLSRMAKLVAGDAQSAVLVLGH
                     ADGSGNAEQSRKLTLERAQAVGSIFRMSGLKNDRLMLRGMGGDKPRASNDNLKGREQN
                     RRIEIILTQRDYLAALVAQNSR"
     gene            16760..17407
                     /gene="pdxH"
                     /locus_tag="VIO83_09170"
     CDS             16760..17407
                     /gene="pdxH"
                     /locus_tag="VIO83_09170"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY8331482.1"
                     /translation="MTQSLADMRRDYTRDGLSEELAPLEPFALFRHWFAEAVKTEQAP
                     VEANAMTLATVDPQGQPHCRVLLLKALDERGFTFFTNYDSAKGEQLLANPRAAMTFFW
                     PTLERQVRIEGRVEKVTPAESDAYFKVRPLGSRLGAWASPQSQVIDGRGELERLLAET
                     ERRFMETAPHCPEHWGGYRLVPERLEFWQGRSSRLHDRLNYRLEGAAWVRERLAP"
     gene            complement(17408..17668)
                     /locus_tag="VIO83_09175"
     CDS             complement(17408..17668)
                     /locus_tag="VIO83_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008719739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331483.1"
                     /translation="MDYFIIFITTVTGLYFHWWLYKRIQRWTDRDLALSFAGDDPAKR
                     AYMLERLQQAQSEGIKRKALGDWLEHAAAQYRPAATATPQQP"
     gene            17770..18231
                     /locus_tag="VIO83_09180"
     CDS             17770..18231
                     /locus_tag="VIO83_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY8331484.1"
                     /translation="MRKSAVFLSLAVAALAVGGCASNLSGETYSRDEVRAVQTVRMGT
                     IAALRPVRIEGTKTPIGAGAGAVAGGIGGSTIGSGRGSAVAAVIGAVAGGLLGAATEE
                     GFTRTQGVEITVREDDGTMRAYVQEVSEGEVFRVGDRVRVLTVNGTSRVAH"
     gene            complement(18381..19376)
                     /locus_tag="VIO83_09185"
     CDS             complement(18381..19376)
                     /locus_tag="VIO83_09185"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY8331485.1"
                     /translation="MLRIAINGYGRIGRAVLRAIFQQGLASRIRVEAINDLGDHATLA
                     HLTRFDSTYGRYPGSVTLHEDCLQISGQSIRLLQERDPGRLPWAELGVDLVLECSGKF
                     KTRALAEQHLLAGAPRVLLSHPLDSADLTVVYGVNHALLGDQQIVSNASCTTNCLAPL
                     AMVLHQAVGIRQGLINTVHSYTNDQNLLDQAHSDLYRARAAALSMVPTSTGAAKTIGL
                     VLPELAGRLDGLSVRVPTPTVSLLDLTFIAERATSVAALRDILLAAIQELPVGVMDCN
                     ELPLVSCDFAGHPASCVVDLSQIRVQGDLVKVLAWYDNERAFALRMLDVAQAWAG"
     gene            complement(19453..20124)
                     /locus_tag="VIO83_09190"
     CDS             complement(19453..20124)
                     /locus_tag="VIO83_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331486.1"
                     /translation="MRIAPVLAVALLPLLGGCQWLPWSAKQPEPAPLRLQGELTRSAG
                     ELVLQPCHQQRRLSITAEDSLGLPYAVARLQQSEQQPLFVDLAGRLQEDASGEQSRLR
                     VNRLYRLQQGAHQGCNDAQFKRLIVRASGNEPGWAVSVSALGMMLERPGVEPLALPYV
                     EERLPDGSLSFTSEADGRRVELWLTPGRCVDSMSGALGHLQARLQLDRQPPLMGCAAL
                     GGARD"
     gene            20313..20597
                     /gene="ihfB"
                     /locus_tag="VIO83_09195"
     CDS             20313..20597
                     /gene="ihfB"
                     /locus_tag="VIO83_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEY8331487.1"
                     /translation="MTKSELIERIVTHQGQLSSRDVELAIKTMLEQMAHALAAGDRIE
                     IRGFGSFSLHYRAPRVGRNPKTGESVRLDGKFVPHFKPGKELRDRVNELE"
     gene            20642..20950
                     /locus_tag="VIO83_09200"
     CDS             20642..20950
                     /locus_tag="VIO83_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEY8331488.1"
                     /translation="MLWIKRVLVIAAALLVAALTLVFILENQGQARLQFMTLQSPEWP
                     VALFVALAFVAGGLAGVLLSLYLRARLKFALARNRSELNRCRKELDGLREQLESKARS
                     "
     gene            complement(20960..22159)
                     /locus_tag="VIO83_09205"
     CDS             complement(20960..22159)
                     /locus_tag="VIO83_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019752439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY8331489.1"
                     /translation="MTSPAKPLAGLKVIELGTLIAGPFASRICAEFGAEVIKVESPDG
                     GDPLRKWRKLYEGTSLWWFVQARNKQSLTLNLKHPEAQAILKRLMQEADILIENFRPG
                     VLEKLGLGWDVLHALNPKLVMVRLSGFGQTGPLKDQPGFGAVGESMGGLRYITGFEDR
                     PPVRTGISIGDSIAALWGVIGALMALRHREVNGGLGQVVDVALYEAVFAMMESLVPEF
                     DVYGFIRERTGNIMPGITPSSIHSSADGKHVQIGANGDAIFRRFMLAIGRVDLADDPS
                     LADNAGRDARRDELYGVIDRWVQSLPLDEVLAALRQADVPASRIYSAEDMFADPQFLA
                     RDMLQRARLPDGKEFRMPGIVPKLSETPGGTEWIGPQLGEHTASILERLGYDPQRIAA
                     LKAQGTV"
     gene            22346..23506
                     /locus_tag="VIO83_09210"
     CDS             22346..23506
                     /locus_tag="VIO83_09210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8331490.1"
                     /translation="MTLHLPTLLLLDICVLALLGGLMLHTWRRSENEATLGWMAGMLL
                     LAAPGTLLLSLRGIGVDTLSIVLGNVLLRLAAGLGWAAMRVFVGRQPCWPGVAGGALL
                     WALLCLWPYFMSSLALRVTVGTLLTILYAGLAAWELWRARRQLEVAIAPAVALLLMHV
                     LFCTGLLLLGSGQAIERVWSGLDDSFVTWRLLETFLFAIGIAFVTLAMVRERAELRLR
                     AVANCDPLTGIGNRRAFMAEAQVLLAYCQRSGRPAALLMCDLDHFKQLNDCHGHAMGD
                     TALIGFGRVLSQSIRQSDVCGRIGGEEFACLLPDADAAQAERVAERIRRECRELMPDP
                     ALRFSVSIGVASVVQAGYELPRLLAQADEALYRAKASGRNRVEHYAGQPMPA"
     gene            23575..24114
                     /locus_tag="VIO83_09215"
     CDS             23575..24114
                     /locus_tag="VIO83_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEY8331491.1"
                     /translation="MALQSTPYKVELNLTDLDRSVYENLRFTVAKHPSETEERMAARL
                     IAYVLWYHEQLSFGRGLSDVDEPALWEKSLDGRVLHWIEVGQPDAERITWCSRRCERF
                     SLVAYGNLRVWQTKVLDGVRSLKNINVVAVDQAALENLAKDMPRAISWSVMISDGSLF
                     ITDERGQHEVPLEWLAGER"
     gene            24201..>24987
                     /locus_tag="VIO83_09220"
     CDS             24201..>24987
                     /locus_tag="VIO83_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEY8331492.1"
                     /translation="MRIEPRPLPAELPDLGDLPPLLTRLYAARGVQSAAELDKTLARL
                     IPYRELKGIDAAVELLVTALEQGQRIVIVGDFDADGATASSVGVLALRMLGAAHVDYL
                     VPNRFEYGYGLTPEIVAVALDKRPQLLITVDNGISSLDGVAAAKAAGLAVLVTDHHLP
                     GPELPAADAIVNPNQPGCPFPSKALAGVGVIFYVMLALRARLRELDWFARRGLAEPNL
                     AELLDLVALGSVADVVPLDANNRILVHQGLARIRAGRARPGLRA"
BASE COUNT         4076 a         7769 c         8662 g         4480 t
ORIGIN      
        1 ctgcggcgcg agatcgtcag cacgcagatc gccaacgatc tggtcaacca catgggcatc
       61 accttcgttc agcggctcag cgaggctacc ggcatgagtg cggccaatgt tgccggtgcc
      121 tatgtgatcg tgcgcgacat cttccatctg ccgcactggt tccggcagat cgaggcgctg
      181 gactacaagg tgccggccga gttgcagctg cggctgatgg acgagctggt ccggctcggc
      241 cgacgggcaa cccgctggtt cctgcgcaac cgtcgcagcg agctggacgc cgctcgcgac
      301 gttgcgcatt tcggcccgcg tgtcgccgcg ctggggctca aactcgacgg tttgctcgaa
      361 ggttcgacgc gcgacctctg gcagtcgcgc taccagcgct acaccgcggc gggtgttccg
      421 gaattgctcg cgcgaatggt cgccggcacc agtcacctgt atacgctgct gccgattctc
      481 gaagcggcag acgaaaccgg gcagccgccg atcaaggtcg cgtcgaccta tttcgccgtc
      541 ggcggtgcgc tggagttgcc ctggtatctg cagcaactca ccagcctgcc ggtggagagc
      601 acctggcagg cactggcacg cgagggtttt cgtgatgatc tcgacagcca gcagcgcgct
      661 atcaccgtgt cgattctgca gatggagagt ggcgcacagc agccggccgc ctgcgtcatg
      721 gaatggctgg agcagcgtgg ctatcacgtc gatcgctggc gcggcatgct ggccgagctg
      781 cgcgccacca acacgcccga ctacgcaatg tacgtcgtag ccagccgcga gctgcaggat
      841 ctggcgcagg cgccagtgag gcatggtgaa taaatggcga gggtggcggc tgaggatagc
      901 cgccactgcg gactgtttta cgagtcttgc ggctggccga gcttgaatcg cgtgatctgc
      961 ggcaggttgc gcagatagcg caccatgtcc ggcgcgccgg cgcgtttcag cccttcgccg
     1021 atagcgctgg cggtagggtg gtgtttctcc agcagcagca attccgagtt gctggcgtta
     1081 cgtagcacgg tcaggcgcgc atacggcacc tgcgtgtcga gcagcagcgc catgaactcc
     1141 gcatcgctcc aggcctgggc gggcattgca atggcgtggt actgctcgtc cagcgcacgc
     1201 agcccggctc ggtcgaggcg gtagacgaca agctcggcag gcatcagcag ttcaaggccg
     1261 cgcaggcgcg cataggccac cgcactggcg aatgcctcga cgtggtgctc tccggcccag
     1321 tacaggcgtt cacccagcca gccggcctgg cgcagctcga tgggctcatc ctgcgcatgg
     1381 ccgggcaggg cgatgccgag ggtacgcagg aagtccttgt agctgatgat gcgggtgcgc
     1441 cggcaggcct cggtggcggc gaatgcttcg atgctcggat aggcgatgcc gtcgggactc
     1501 tggccgccga gaccgtcgat ctggcgcagg tcgatgctgg ccagcgactg actgtctttc
     1561 tgaatcaacc ggctcagcgc agcgtgcgcg cgcgccttgt cttcctgtac cggacccgaa
     1621 agtgcaccgc gcggcagctc cagcaggcgc tgcaggcagg ggccgtcatt ccagaaaatg
     1681 ctcggctccg gctcgtaaag cggcgcgaaa ggcaggttca gccgactcgc acgctccagc
     1741 gtctgatggc cgggttgtgc gcgtagcccc agccgctggg ccaggcgggt gatgtgagtg
     1801 gacaatgaag gcatggactc gggcaggctc gtcacgatgc ttgagctcgg aaagtactct
     1861 ggggagtgtg gcagaccgag ccgcgaagcg cacctgcctt gcgcgcttca tgcgccgggc
     1921 agcagcggac cgattggcta tggcggccgg aacggcgcgc cgatgtgcga ggattgctgc
     1981 aattgaacac gaggagcctc catgcctagc ctggtgctgg atattgcact gcctgccgag
     2041 cgcctgctgg ctgcctatca agggcacgcc aaccgggttc tgctgcgcag ccgcgacggc
     2101 agacgaatca gcctaccggt gcatcatctg cggccctttg tgactcgcga aggggtattt
     2161 ggttccttcg agctggaatt tgcccagcaa ggccagctga tcggcctgca ccgcttgcgc
     2221 tgaagcgcct tcggagcagc cgcgcaggcg gctgaaatac cgcgtcgcct gctatactcc
     2281 gcggccatca cttcccgaga ttctaggctg cgtatgtata acctggcccg ccagatgctg
     2341 ttcaagctgt ccccggaaac gtctcacgag ctgtcgctcg atcttatcgg cgccggcggc
     2401 cgcctgggta tcaatcggtt gctgagcaaa tcgccggcaa gcctgccggt gcgcgtcatg
     2461 ggcatcgatt tcccgaaccc ggtcgggctc gccgcaggcc tggacaagaa cggcgaggcc
     2521 atttgcgggt tgtcgcagct gggcttcggt ttcatcgaag tcggcacggt cacgccacgc
     2581 ccgcagccgg gcaatccgaa gccgcgcatt ttccgtctgc cggaagccga ggcgatcatc
     2641 aaccgcatgg gcttcaacaa ccagggcgtc gatgcgctgc ttgcgcgggt ggacgccgcg
     2701 cgcttcaagg gcgtgctggg aatcaacatc ggcaagaatt tcgatacgcc agtggagcgt
     2761 gccgacgagg attacctgct gtgcctggac aaggtttacc accatgccag ctatgtgacc
     2821 gtcaacgtga gctcccccaa tacgccgggg ctgcgcagcc tgcagttcgg cgactcgctc
     2881 aagcgtctgc tcgaagcgct gcgcctgcgc cgcgaggatc tggagatcga gcatggcaag
     2941 cgcgtgccgc tggcgatcaa gatcgccccg gacatgaccg acgaggaaac cgtgctggtg
     3001 gccgaggcgg ttctgcaggc tgggatggac gcgatcatcg cgaccaatac tacgctgagc
     3061 cgtgagggtg tcgcggggct ggcgcacgcc gacgaagcag ggggattgtc gggcgctccg
     3121 gtgcgcgaga aatccaccca caccgtgcgc gtgttggcgc aaaccctcag tggccggctg
     3181 ccaatcatcg ccgtgggcgg gatcaccgag gggcgccacg ccgccgagaa gatcgccgcc
     3241 ggagcgagcc tggttcagct gtattcggga ttcatctaca aggggccggc gctgattcgc
     3301 gaggcggtgg atgcgattgc cgagttgcgc gcgaaggcgc agtgactaag aggtcggaat
     3361 cgagatcgga atcggcccga tgcggggctc cggatcggag cccctgggct tctagcccgc
     3421 cgcccggatg gggcgtgcga tgaagagtcg gtcaggtcgg ggaatcagcc gaccgcgtgg
     3481 agttcgttca gtcgatgcag accggcggtg ccggtcagac catcccagtt gtcgccgcga
     3541 ccctcccgcc agccgttgat ccaggcttgg cgcacagtgg gatgggtgaa aggacacagg
     3601 tcgcgagatt tgccatgtat gccgtgttga taaccgcgca ggaatgctcg ttccatcgga
     3661 tcacgcttga gtcttctcat tgggtattgc cctcagtggt tgactggtgt tcctggtggc
     3721 ctcatggcga gaccgggcag acaggctgcc gttgggtccg ctgccggcgt ggcggacgcc
     3781 atcgacctca ttgcgatggt cgattgggac gagttctaac ccaatggccg agaggctgga
     3841 atgatcgttt tgtcataagt aggtaaccaa actatggttc agcccataag cagtgtgtac
     3901 tgccgggcct agagccaccc gcccgcaacc gcgcgacagc gccggcgcgc gtctgtgtga
     3961 cgcagggcaa tttttttgct gggccattcc gacgaagggt cggatcgttc cgcttcgaac
     4021 actcactgga agcatccatg accgaccgtc acgaactcat tctcacctgt cccaagggac
     4081 tcgaaggctt gttgctggag gaagcccggc aattggggct ggaggacgcg cgtgagcaga
     4141 ccgcggcaat ccgcggtttc ggcgccctgg aagtcgcgta tcgtctgtgt ctctggtcgc
     4201 gcctggccaa tcgcgtgctg ctggtgatca gccgctttcc gaccacgacc gctgagaccc
     4261 tttacgaagg cgtgcgcagc gtggactggg ccgagcatct ggaacctggc ggcagcctgg
     4321 cagtcgagtt cagcgggcgc gggtcgggta tcgacaacac tcatttcggc gcgctcaagg
     4381 tcaaggatgg catcgtcgac cgtctgcgca ccgccagcgg cgagcgaccc acggtcgaca
     4441 agctcaaccc tgatctgcgc gtgcacctgc gcctggacaa gggcgaggcg gtgctgtcgc
     4501 tcgatctgtc cgggcacagc ctgcatcagc gcggttatcg cctacagcag ggcgctgcgc
     4561 ctctcaagga aaacctggcg gcggcggtgc tgatccgcgc cggctggccg cgcattgcgg
     4621 cggaaggcgg cgcgctgacc gatccgatgt gcggcgtggg tactttcctc atcgagggcg
     4681 ccatgatcgc cgcggacatc gcgcccaacc tcaagcgcga gcgctggggc tttagcgcct
     4741 ggctcggaca tgtgccggcg ctatggcacc gcttgcatga tgaggccgag gctcgcgctg
     4801 cggccggtct ggcgcgtccg ccattgtgga ttcggggcta cgaggccgat ccgcgcctga
     4861 tccagccagg acgcaacaac atcgagcgtg ccgggctggg gagctggatt cgcatctatc
     4921 agggtgatgt ggcgacgttc gagccgcgtc ccgaccagaa ccagaaggga ctggtgatct
     4981 gcaacccgcc ctatggcgag cgcctgggcg atgaggcaag cctgctgtat ctctaccaga
     5041 acctcggcga gcgtctgcgt caggcctgcc tcggctggga ggcggggatc tttaccgcgg
     5101 cgccggacct gggcaagcgc atgggcattc gcagccacaa gcagtacgcc ttctggaacg
     5161 gtgcgctgcc gtgcaagctg ctgctgatca aggtcgagct cgaccagttc gtgaccggca
     5221 atcgtaccgc ggcaccacag gcgaacgccg cgccggcgcc ggttgaagtt gcgcggctga
     5281 gcgaaggcgg gcagatgttt gccaatcgcc tgcagaagaa cctgcgccag ttgggcaaat
     5341 gggcgcgccg cgagggcatc gagtgctacc ggctgtacga cgccgacatg cccgaatacg
     5401 ccgtggcggt ggacctgtat cgtgactggg tacatgtgca ggagtacgcg ccgccgcgct
     5461 cgatcgaccc ggacaaggcc caggcgcgcc tgctcgacgc cctggcggcg attccgcagg
     5521 cgctggacat ggatccttcg tgggtggtga tcaagcgccg cgagcgtcag gccggcaaga
     5581 agcagtacga gcgccaggac cagcaggggc gcttcatgga ggtcagcgag ggtggggtga
     5641 agctgctggt caacctcacc gactacctgg ataccggcct gttcctcgac caccgtccgc
     5701 tgcgcctgcg catccagcgc gaagcggccg gcaagcgctt cctcaacctg tactgctaca
     5761 cggcgacggc caccgtgcat gcggccaagg gcggtgcgcg cagcaccacc agcgtcgacc
     5821 tgtcgaagac ctacctggac tgggcgcgcc gcaacctggc gctcaacggc ttctccgaca
     5881 agcacaagct cgagcagggc gacgtgatgg agtggctgga ggccgatcgc ggcgaatacg
     5941 agctgatctt catcgatccg ccgaccttct ccaactccaa gcgcatggag ggggtgttcg
     6001 acatccagcg cgaccatgtg cgcctgctgg atctggccat ggcgcgcctg gccaggggtg
     6061 gggtgctgta cttctccaac aacttccgca agttccagct cgacccgtcg ctggaggagc
     6121 gctacgcggt ggaggagatc agcgcgcaga ccctcgatcc ggacttcgtc cgcaacccga
     6181 agatccaccg cgcctggtgc ctgagcgcgc gccagggggt gtaatccggg taggggctgg
     6241 gcggtggaaa agacctgcgg tcgttttcca ccctaccgga ctgccgaact gccgcgcatg
     6301 aaaaagccgc ctgcccggtg tcccgggcag gcggcttttt cgtgtcgggc tcagcgcccg
     6361 gcgttggcgc tgagggcgcc cggttgccgg tagagctcct ggaggatttg atcgaggatc
     6421 gccgaggcgc cccacggccg cgggtggttg aggatcgcca ccaccgccca ggtatggcct
     6481 tgggcgtcgc gactgaagcc ggccaaggcg cggacgttgt tcagcgtgcc ggtcttcagg
     6541 tgcgcgttgc cggccagcgg ggtgttgcgc atgcgcttgc gcagggtacc gtccatcgcc
     6601 gccagtggca gcgaggagat gtattcggcg gcatacgggc tgttccatgc cgcatgcagc
     6661 atggcggcca tttcccgcgc gctgatgcgc tcgttgcgcg acagtcccga accgttctcc
     6721 agcaccaggt gcggcgcgcg gatgcccttg cgcgtcagcc actggtcgat cacccggtag
     6781 gctgccgcgg catcgtcgga gtcactgggc aggcggtggc gcgcgccgat cgacaggaac
     6841 agctgacggg ccatggtgtt gttgctgtac ttgttgatgt cgcgaatgat ctccaccacg
     6901 tccggcgacc aggcctgagc cagcaggcgc gcgttgcccg gggtggcggc caggcgatcc
     6961 tgtccctgga tgctgccgcc cagttcgctc cacagcgcgc gcacggcgcc ggcactgaag
     7021 gtcggatggt cgagcagcga catgtagcgc tggccgctgc agccttccgg gatctggccg
     7081 gtagccacca gggtcagacc gctgccctgc tgcggctgga cgttgtagtt cacctgcggc
     7141 cagctcgggc aggccgcggg cggggtcacg cgcacccggt tgtcgatggt gatgtacgga
     7201 atcggcggct cgacctgcac ggcggcgccg cgtgcctcgc cgcggacgat caggcgcagg
     7261 gtcttgaggt tgaccatcag cgggtccggc tcgaccagat agggcttgct ggagtcgccg
     7321 ccatcgtcgt tgaacgaggt atgcagtgcc ggacggaagt ggttgcgctc cagtaccagg
     7381 tcaccctgca cctgctgcac gccggcgttg cgcaggtcgc gcagcagcag ccagaggcgc
     7441 tccatggtca gcttcgggtc gccgccgccc ttgaggtaga ggttgccctg cagcacgccg
     7501 ttgcgcagcg ggccgtcggt gtagaagccg gtcttccact ggtaggtcgg gccgagcagc
     7561 tcgagggcgg cataggtggt caccagcttc atggtcgagg ccgggttcac cggcgcgtcg
     7621 gcgttgaagt agctggcggt gccggggccg tcgagcggga tcagcgccag cgacagcgca
     7681 tcgctgttga tcttgctggc cttgagcgcc tgctccaccc gtggcggcag cgctgcctca
     7741 gcggcgggca gcggcaggat catgggcagg aacagaccgg cgaggccgag caggcgcagc
     7801 ggtcggacga ttttcaatgc cttggcgagc aaggacttgg gcgcgcggaa caacacgaga
     7861 gggctcacat tcgcggtttg aataatattt ctcattatgc ccgaaacgcc ctataacggg
     7921 gctttggcgt gccggacggc acgcctgtcg tgggcctgac tggcagaagc tggtagagtg
     7981 ccgcacctcc accccttttc aaggatgccc tcatggctac caatcgctcc cgccgtctgc
     8041 gcaagaagct ctgtgtcgac gagttccagg aactcggctt cgagctgctg ctcaactatc
     8101 gcgaagacct cgacgaagtc gccgtcgatg ctttcctggg gcagttcctc aaggaagcga
     8161 tcagcgacaa cgacatgatc ttcatcggct gcgacgagtt cggcatggtc tgcctgggca
     8221 agcgtggctc ggtaaacgaa gagcagcgcg ccaaggtcga cgcctggctc aagggccgca
     8281 gcgagctgga aagctacacc ctcggcaagc tggccgatgc ctggtacccg gaaaagccga
     8341 tcaacgcctg atctgatcgc ctgctccaga caaaagcccc gcgccacgtc agtgtcgcgg
     8401 ggcttttgca tgttcaggac atcacaccgg gctggagcgg ccggtcatct cgcgggccat
     8461 ctcggtggcg tagctgtcgg tcatcccggc gatgaagtcg atcacccgca tgtacgcctt
     8521 gtacagcgac cagtgggggt cgggcgcgtt gttgccgagc aggtcgagga tgcgccggtt
     8581 cttgaacgac ggcgtgcgcc cgccatgctg ttccagcacg gcgccgcaaa aggcgttgag
     8641 gaggatctcc agggtggtat aggcgccgat ctcgtgcagg gtcttgcgct tgtcctggaa
     8701 gatcttttcc cgcgccaggg ccttggcgcg cagcacgcac tgcttggccg ggccgtgcat
     8761 gtgctcgacc agatcgccct ccaggctgcc ggcgagcagg gcgtcgtgct gggccacgaa
     8821 ggcgcgggcg gcggcgttgg tcaggtgctc gatggccttg ccgcgcagga tcgccagctt
     8881 gcgccggcgc gagtcgcgcg ggccgagctg gcggtaggtc tccggcaggt cgtcgccgac
     8941 caggtcgagg agcagcgcct cgacttccgg gtagtccagc aggtccattt ccaggccgtc
     9001 ttccaggtcg atcaggccgt agcagatgtc gtcggccgcc tccatcagat acaccagtgg
     9061 atggcgtgcc cagcgttcgt tttccagctg tggcagcccc agcttgcggg cgatctgctc
     9121 gagcagcggc agctcgctct gatagcagcc gaacttgtgc ttcttgtagc cctgggcgtc
     9181 ggcgtggcgg gcggtccagg ggtacttcag gtagctgccg agggtggcgt aggtcagccg
     9241 ggtgccgccg tcgaactggt ggtactccag ctgggtcagc acgcgaaagc cctgggcatt
     9301 gccctcgaaa ccgaggaagt cggcgcgctc ggcctcgctc agggcatcca gccagccgcg
     9361 ttcgtcggcc tgctggaacc agtggcggat ggcgtcctcg ccggagtggc cgaacggcgg
     9421 gttgccgatg tcgtgggcca ggcaggcgga ctgcacgatc acgcccagat cgccggggtc
     9481 gcaccagcgt ggcagcgcct cgcgcagcat ctcgccgacg cgcatgccca gcgagcggcc
     9541 gacgcaggcg acttccaggc tgtgggtcag gcgggtgtgg atatggtcgt tgctggatac
     9601 cgggtgcacc tgggtcttgc gccccaggcg gcggaaggcg ccggaaaaga tgatgcggtc
     9661 gtggtccttg tggaaggcgc tacggccgag ctcttccgag ctctgtacgg gtttgccgaa
     9721 gcgttcgcgg gtaagcaggg tgtgccagtc caaagcggat cctccgcagt cggggacttc
     9781 cagcttgcgg gctgcgcggt cgccgtgcaa gttccgcaat gccgcttcgg tgctctattt
     9841 cggtgctcaa gattgtctgg aagaggctgc cacagcacca gagttccgta acaggctccg
     9901 cggtacgcgc agtttcagtg ccggctctgg ccttgattga tgcatgaact ctgccttgga
     9961 gggttctttg cgcgtgagac gagtgcaagt gcgctgtttt gggtgatttc atgcgcgtga
    10021 tggctgcgtt tctgctggct catggtgcgt gccggcatag tgcttgcatt aatttaatgc
    10081 gtgtgcacct gcgccggcct tgcgccgtgg ctgggtgatg actgacagga tgttgccaca
    10141 cacattcaac cccttgattt tcaagaaccc gcaaccgagg ctggccttgc cgaccggtgt
    10201 ttcctggccg acgggtctat cgtgatcaat gcttcaggat ggtgcgcgcg ctcgtgtgta
    10261 gtgcgtggat ggctgaagat gattcgcctt ggtgccttcc tgtcccagct gaggagcgca
    10321 ttgcgctgat ggaaaacctg catagtgccg tggaaagcct gatccacggg tcgaataccc
    10381 tattcatcct gctcggcgcg gtcatggtgt tggccatgca tgccggcttt gcctttctgg
    10441 aagtcggcac cgtccgccag aagaaccagg tcaacgcgct gtcgaagatc cttgccgact
    10501 tcgcagtctc cagcctggcc tacttcttca tcggctactg ggtggcctat ggagtgaact
    10561 ttttcgggcc ggccggtgcg atgaccgccg acaacggtta tgcgctggtg aaattcttct
    10621 tcctgctgac ctttgccgcg gcgattccgg cgattatttc cggcgggatc gcggagcgtt
    10681 ccaagttcgg cccgcagctc tgtgcgaccg tgttgatcgt ctccttcgtc tatccgctct
    10741 tcgagggcat ggtctggaat ggcaatttcg gctttcaggc ctggctgctg gagcgcttcg
    10801 gtgcctcctt ccacgacttc gccggctcgg tagtggtgca tgcgttcggt ggctggctgg
    10861 cgctgggggc tgtgctgatg ctcggtacgc gcaatggtcg ctatcgcgac ggccggctgg
    10921 tggcgttcgc tccgtcgaac atcccgttcc tggcgttggg ctcgtggatc ctgatcgtcg
    10981 gctggttcgg cttcaacgtg atgagtgcac agacgctgca gggcgtcagt ggtctggtgg
    11041 cggtcaattc gctgctggcc atggtcggcg gcaccctggc ggcgctgctg gtcgggcgca
    11101 acgaccccgg cttcctgcac aacgggccgc tggctggcct ggtggcggtg tgtgccggct
    11161 ccgacctgat gcacccggtc ggtgcgctgt tcaccggagt ggtggcgggg gcgatgttcg
    11221 tctgggcttt caccgctacg caaaaccgct ggaagatcga tgacgtgctc ggcgtctggc
    11281 cgttgcatgg cctgtgcggc ctgtggggcg gcattgcctg cgggattttc ggccagcagg
    11341 cgctgggcgg cgtgggcgga gtcagcctgg tcagccagtt gctcggcacc ggactgggcg
    11401 tcgcgctggc gctggccggc ggcttgctgg tctacggtgt gctgaagagc acgctgggta
    11461 tccgcctgag tgcggaggag gagtactacg gggcggacct gtcgatccac aagatcggtg
    11521 ccaccagcca cgattaaacc gcttggccgg aaacgaagaa gcccgctcga gagcgggctt
    11581 cttcgttatt gccggacggc ttagagcagc ggcagggtgt agctgacgat cagacggttt
    11641 tcgtcgaggt cgctggcgaa gttggaacgc acggtggcgt tgcgccagtg gataccgagg
    11701 ttcttcaggg tgccttcctg gaacacgtac ttcagctcgc tgtcgcgttc ccactccttg
    11761 ccttcggcgc gagtgccgcc cagctcgaca ttgtcgccgc tgaggtagcg ggtcatgaag
    11821 gtcaggccgg gaatgccgac ggtggcgaag ttgaagtcgt agcgggcctg ccaggacttc
    11881 tcgtcgcggt tggcgaagtc gctgatctgc acatagttga ccaggaacgg gtcggtggag
    11941 tcgccgacat aggcgtagcc ggtgtcgccg ctcatcgact ggtagcccag gccgaagcta
    12001 tggccttgca gggtgtaggt gaacagggcg cccatggcct tgttgtcgac gttgctggat
    12061 tcgtcgtcca gcgagcgggc gtagcgcagg tcgctcttga agctctggcc gtcggcgatc
    12121 ggcagtacgt gcaccacgtt gaagatgtgc tggcgataga actcgtccag gttgccgtag
    12181 tggtaggcag tggtcagctg gctgttccac ttgtagctgg cgccggcgaa ggtcatggca
    12241 tcgctgtcgg tgccaggagc aaagcggata ttgcgccggc cgccactggt aatgaccatg
    12301 gcttgatggt cctgcgaatc gcggttgttg atgcgattca gctggccgat gtccagggtc
    12361 agaccggcga tttcctggga gttcaggtgg gcgccctgga agctctgcgg cagcaggcgc
    12421 gactcgctgg cggatacgct cggcagcttc ggaatcaggg tgccgacctt cagcacgctg
    12481 ttggaagcct tgaacttggc agtgacaccc agatcgccgt agctgtcctg gggctggccg
    12541 gtttcacggt cgcgcaggag gaggccggta ccgctggtgc cgcggccgga gtcgagctgg
    12601 agacccaagg tgccgatggc atccacgccc atgccgatgg tgccttcggt gtagccggac
    12661 tccatgcgca ggatgaaacc ttgcgcccat tcttcagcct tgtggttgcc ggaagtattg
    12721 ctgcggaaat cacggttgaa gtagaagttc ttcagctcca ggctggcctt gctgtctttg
    12781 acgaagtcgg cctgggccag gctcggcagg atcatgccgg cgcccagggt cgccagtgca
    12841 acggcgcgag cgaggggagt caagctcatt ggggtgtgct ccttgttttt tttagtcgta
    12901 catcaggggt aaccctgatg tttgtaaaac tatgtgtgaa ttaaacacga cattatgacg
    12961 gcggctctta tacgttggta tatgtttttc ctgttttgcc gacgccatcg tggaacgatg
    13021 agggcttgtc cgataattaa atagttaatc cagcgtcatt aactatttaa caaaatcgcc
    13081 tgcttatcga atgcctgcca gcagccaggc ttcggcgctt tcgatggcat ccagcgggcc
    13141 ggccagcagc agggcatcgc cttcgcacag ggcatggttg ccgggtagtg ctagctctcc
    13201 gccatcgcgg cgtaccgcct gtagcgcaac gcccatcgcc ggcaggtcca gttcggccag
    13261 tgtgcgaccg caggcatagg cgtcggcgct caggttgacc gcgtgcagca aggtgcgcgg
    13321 tcgcccctgc tgatcgagca ggttggcgct ggcgccgtga tagaagccat gcagcatgtg
    13381 gtagcggctg cggcgtacct cgtcgatgcg ggcctgtacg cgccgctctg gcaggccgag
    13441 catgaccagg gcatgggagg cgagcatcag gctcgactcc agcagctccg ggaccacttc
    13501 gctggcgccg gcagcttgca ggctggccag gtgactgtcg tcgcgggtgc gcaccaggat
    13561 cggcagggtt tcggagaatt tgcgcgcctc cttgaccgtt gccagagcgt gctcgggatt
    13621 gtcggcggca atcaccagca gccgcgcgcg cgccaccccg agtgcagtca gcagttcgcc
    13681 acgacgtgcg tcgccgaagt gcacgctgtg ttcgccggcg gcagcctctt gtacgcggac
    13741 cgggtcgttg tccagcgcca cgaaggggat gccctcgctg cgcaggaagc ggccgatcga
    13801 ctggccgacc cggccgaaac cgcagatcag gacatgctga tcaagctcgg tacaggcagc
    13861 ggcaatctcc ctggcactac cttcggcatg cgggcgctgg ccgagacggg cgaccaggtg
    13921 tggcgcagcg cgcagcagca gcggggtcac ggccattgaa cagaaggtgg cggccaagat
    13981 cagtccgccg gtctcgatcg acaccagcct ggcctgctgg gcctgggaga ccagggcgaa
    14041 gcagaactcg ccgccctggg ccagcgccag tccgctgcgc caggcgcttt cactgctgtc
    14101 gccgcgcaga cgcaccagca gcgccaccag actgcccttg agcagtagca agagcaccgc
    14161 catgcccagg atcagcaggc cgtgggaggc aaacaggctg aggtcgatga gcatgccgac
    14221 gctgacgaag aacaggccga ggagaatgtc gcggaacggg cgaatgtcgg cttcgatctg
    14281 gtgccgatag tggctctcgc ccagcaacat gccggcgagg aaggcgccca gcgccatgga
    14341 caggcccagc aggtgggtca gccaagcggt gagcagcacg atcaccagtg ccagcagcac
    14401 gaacagttcg gcggcattgg cctgggctac ctcgtgaaac agccgcggca gcagccagcg
    14461 gctggccacc agcagggccg tgaacagcag tgcggccttg cccagagtga tcggcaacgc
    14521 ccagtaccag gtctgttcgg cgccgccggc gatcaccggg atcatggtca gcaacagtac
    14581 tgcaaccagg tcctggaaca gcagcacgcc gatggcgttc tgcccgtgag gggtgaacac
    14641 ttcgccgagg ctgcccagtt ccttggtgac gatggcggtg gaggacagcg ccagtccgcc
    14701 tcccagcagc agtgcggccg gcagcgtcag gcctgcgagg tggagcaggc cggccagcgg
    14761 cagggtgcag gcgatgacct gcaggctgcc gagcccgaac actacccggc gcagggcgag
    14821 catgcgcggc agggaaaact ccaggccgag actgaacagc aggaacacca cgccgaactc
    14881 ggcgagcagc ggcatgtcct cgtgctgttc gagccagccc aggctgccgg ggccgagcgc
    14941 caggccaacg ctcatgtagc ccagcagggg cggcagcttg aggcggcgaa acagggcgat
    15001 gaccagcagc gaggcggcca ggatgatcag tagattgacg aacactgata gctctctaga
    15061 gacgaaaggg gcacaaagcc cccgcagggt gcccaatcca tggtgccagc gtccagagcg
    15121 gcctgggttg gtgtgcgcca tcgggcggtt gtcgcgcatg ttaaactgcc gtctcggccc
    15181 ttcaccatga gcgacgtcat gctgcccgat tgcctgctgt tcggaaccct cggctgtcac
    15241 ctgtgcgaac aggcggagga gcagctcatg ccttttgtcg gcttggggct gcaggtcgag
    15301 ctggtcgaca tcgccgagcg cgaggagtgg gtcgaggcct atggtctgcg catccccgtc
    15361 ctgcgccgag tcgacaccgc agccgagctg ggctggccgt tcgatggcca gtctgtggcg
    15421 gagtttctcg gcctcccgcc gggtccggca ggcggctgat acggccactg gccagcagta
    15481 gctacctgcc gttcgatacg gcactatctg cccgtttttg ggccggcacc tatactactg
    15541 atgcctaggc gtcgcgcgtt gtgcgctgag cacctgggcc aatcatccgg agccgctggc
    15601 ggggttgcct gaaaaggtct ctggcccgca agcgttctgt ctcgttcaac tggaaatgat
    15661 catgtctctc gtaaaacctg cagctctgtt cgtgtgtgtc gctctggccg gttgtgccag
    15721 caagtccgat tccggtaacg cttcggccaa ggatggctgg cggtcgttcg gtgggaagga
    15781 gacggcggct cgtgcagagc aaggcaagtc cgcagaggct gcaaagcagg tggaggacaa
    15841 ggccgagctc gccccggcca agccggtcga gaagaagccg caaacggccg cgaagaccgg
    15901 gcagaagccg gcgccggtag catcccgggt tgagtcgaag aaggccgcgc cggtcgctgc
    15961 caagctcgag cagaagaaag ccgcgcagaa gccggaaccg actgcagtga gcaaggtcga
    16021 gccgaaggca gagccggctg cggcgcaacc ggctgacgac aatgccctga aggccacgca
    16081 ggccgaggtc aagaaaaccg aggtcaagaa ggcaggcagc agctggtggc ggtttggtgg
    16141 caaggacaag gcagtcgaca cccaggccca gctccagtcg aagaagagcg agcgccagcc
    16201 ggtcagcaag gcctggctgg acgacaacga gaagcgcctg aaagccgcgg tggcaggcag
    16261 caagttcgag gtcgagcgtc gcggtgacct gctggtgctg gtggcaccgg cggatacgtc
    16321 cttcaatcgt gatcgcccgc acatgctgct gccggtcacg ctcgggccgt tgagccggat
    16381 ggccaagctg gtcgcgggcg atgcgcagag cgccgtgctg gtgctggggc acgccgatgg
    16441 cagcggtaac gcagagcaga gccgcaagct gaccctggag cgggcgcagg cggtggggtc
    16501 gatcttccgc atgagcggcc tgaagaacga ccggctgatg ctgcgcggaa tgggcgggga
    16561 caagccgcgc gcgagcaatg acaacctcaa ggggcgtgag cagaaccgtc ggatcgagat
    16621 catcctgacc cagcgtgact acctggcggc gctggtggcg cagaacagcc gctgagtggc
    16681 gatggccttg ctgtcgatca tgggcggcaa ggccgagccg gggttaagct gacacattca
    16741 tcgcccaagg agttctgcca tgacccagtc gcttgccgac atgcgccgcg attacacccg
    16801 ggacggattg tccgaggagc tggcgccgct ggagccgttc gcgttgttcc gccactggtt
    16861 tgccgaggcg gtcaagaccg aacaggcgcc agtggaggcc aatgccatga ccctggccac
    16921 ggtcgatccg caggggcagc cgcattgccg ggtattgctg ctcaaggcgc tggacgagcg
    16981 tggcttcacc ttcttcacca actacgacag cgccaagggc gaacagctgc tggccaatcc
    17041 gcgcgcggcg atgaccttct tctggccaac cctggagcgc caggtgcgca tcgaggggcg
    17101 ggtggagaag gtgacgccgg ctgaatcgga cgcctatttc aaggtgcgtc cgctgggcag
    17161 ccggctgggt gcctgggctt cgccgcagag ccaggttatc gacgggcgcg gtgagctgga
    17221 acggctgttg gccgagaccg agcggcgctt catggaaacc gcgccgcact gtccggagca
    17281 ttggggcggt taccggctgg tgccggagcg cttggagttc tggcagggac ggtcgagccg
    17341 cctgcacgac cgtctaaact atcgtcttga aggggctgcg tgggtgcgtg agcgcctggc
    17401 gccctgatca gggctgctgt ggcgttgcgg ttgctgcagg ccggtattgg gctgcagcat
    17461 gctccagcca gtcgccgagg gctttgcgct tgatgccttc gctctgtgcc tgttgcaggc
    17521 gctcgagcat gtaggcgcgt ttggccgggt catcgccggc gaaggacagg gccaggtcgc
    17581 gatcggtcca gcgctggatc cgcttgtaca gccaccagtg gaagtacaaa ccggttacag
    17641 tggtaatgaa gatgatgaag tagtccatat cgggctccca gaaaggcgtg cccaccctag
    17701 ccgatcatgg cgcgctggaa aagcgctggc tccgggcggg caaggcggca agcaaaacgg
    17761 agaagtgtca tgcgcaaatc agctgtattc ctgagtctgg ccgtggcggc cctggcagtg
    17821 ggtggttgtg cctccaacct gagcggcgaa acctattcgc gcgatgaggt acgggcggta
    17881 caaaccgtgc gcatggggac tatcgccgcg ttgcgtccgg tacgtatcga gggcaccaag
    17941 acgccgatcg gcgccggggc tggcgcggta gccggcggca tcggcggcag cacgatcggc
    18001 agcgggcgcg gcagtgccgt ggctgcggtg atcggtgccg tggccggtgg tctgctcggt
    18061 gcggcaaccg aggagggctt cacccgtact cagggtgtgg aaatcaccgt gcgcgaggac
    18121 gatggcacca tgcgcgccta tgtccaggaa gtgtccgagg gcgaagtctt ccgtgttggc
    18181 gaccgggtgc gtgtcctgac cgtcaacggc accagccgcg tggctcactg agtctgttgc
    18241 ttgccgctac agctcattgc tcgcctgccc tggaggggca ggtgagcatc tgttgagtgc
    18301 ttggcaagat cccgcatagc gcacgccgac tggtctggct gcgtccccct tctgctcgca
    18361 ccgagccgcc accgtcttat tcagccggcc caagcctggg ctacgtccag catgcgcagg
    18421 gcgaatgcgc gctcgttgtc gtaccaggcc agcaccttga ccaggtcgcc ctgcacgcgg
    18481 atttgactga ggtcgaccac gcaggaggcg gggtggccgg cgaagtcgca ggacaccagg
    18541 ggcagttcgt tgcagtccat gacgccgacc ggcagttcct gtatggcggc cagcaggatg
    18601 tcgcgcaggg cggcaacgct ggtcgcgcgc tcggcgatga aggtcaggtc cagcagcgat
    18661 accgtgggtg tcggtacgcg caccgacagg ccgtccagcc gtccggccag ttctggcagc
    18721 accaggccga tggtcttggc cgcgccggtg ctggtcggca ccatcgacag cgccgccgcc
    18781 cgtgcgcggt acaggtcgct atgcgcctgg tcgagcaggt tctggtcgtt ggtgtaggag
    18841 tgcacggtgt tgatcagtcc ttggcggatg cccacggcct ggtgcaggac catcgccagc
    18901 ggagccaggc agttggtggt gcaggaggcg ttggaaacga tctgctggtc gcccagcagc
    18961 gcatggttga cgccgtagac cacggtgagg tcggcgctgt ccagcggatg actgagcagc
    19021 acccgcggcg cgccggcgag caggtgctgt tcggccagcg cgcgggtctt gaacttgccg
    19081 ctgcattcga gcaccagatc gacgcccagt tcggcccatg gcaggcggcc gggatccctt
    19141 tcctgcaaca gtcggatcga ctggccgctg atctgcaggc agtcctcgtg cagcgtcacg
    19201 ctgccgggat agcggccgta ggtcgagtcg aagcgggtga ggtgggcgag ggtggcgtgg
    19261 tcgccgagat cattgatcgc ctcgacgcgt atgcgtgacg ccagtccctg ctggaagatg
    19321 gcgcgcagca ctgcgcggcc gatgcggcca taaccgttga ttgcgatacg cagcatggct
    19381 ttgactccct tttgttcttc tcattcagga gcatagctgc ttgtgctgcg gcccgtggca
    19441 cgctccggcc gctcagtcgc gcgcgccgcc cagcgctgcg cagcccatca gcggcggctg
    19501 gcggtccagc tgcaggcgcg cctgcaggtg gccgagtgca ccgctcatgc tgtcgacaca
    19561 gcgccccggc gtcagccaca gttccacgcg gcggccgtcg gcttcgctgg tgaagctcag
    19621 gctgccgtcc ggcaggcgct cctcgacata gggcagggcc aggggctcga cgccggggcg
    19681 ctcgagcatc atgcccaggg cgctcacgct gaccgcccag cccggctcgt tgccactggc
    19741 gcgcacgatc aggcgcttga actgggcatc gttgcagccc tggtgcgccc cctgttgcag
    19801 gcgatacagg cggttgaccc gcaggcgcga ctgctcgccg ctggcgtcct cctgcagccg
    19861 accggcgagg tcgacgaaca ggggttgttg ctcactctgt tgcaggcggg ccacggcata
    19921 gggcagcccc aggctgtcct cggcggtgat ggacagcctg cgttgctggt ggcacggctg
    19981 cagcaccagt tcgcccgcgc tgcgggtcag ctcaccctgc aggcgcagtg gcgcaggttc
    20041 cggttgtttc gccgaccacg gcagccactg gcagccgccc agaaggggca gcagcgccac
    20101 ggccagtaca ggtgcaatac gcatgacaac tctccctagc agaatggtcg ccacggtaat
    20161 cgccgccgcg ggcggccaca agaagcttca ggcctaagcg gcaggtcaca caatcgcttc
    20221 gtgctgtcaa tagcaatggg ctggtcaaac cccgtggagc atgctacaaa cattcaaaga
    20281 tgatctagcc gcttgaaaca caagggaaaa ccatgaccaa gtcggagttg atcgaacgca
    20341 tcgttaccca tcaggggcaa ctgtcgtcca gggatgtcga gctggccatc aagactatgc
    20401 ttgagcagat ggcgcatgcc ttggccgctg gtgatcgcat cgagattcgc ggcttcggca
    20461 gcttctccct gcattaccgc gcgccgcgcg tcgggcgcaa tcccaagacc ggcgagtcgg
    20521 tgcgtctgga tggcaagttc gttccccact tcaagcccgg caaggagctg cgcgaccgcg
    20581 tcaacgaatt ggaatgacgt tcacgcggcc tgcaccggtc ggccccgttc gaggaaagcc
    20641 catgctctgg atcaaacgcg tcttggtgat tgctgctgcc ctgctggtcg cggcgctcac
    20701 gctggtcttc atcctcgaga accaggggca ggcccgcttg cagttcatga cgctgcaaag
    20761 ccccgaatgg ccggtcgcgc tcttcgtcgc cctggcgttc gtcgccggcg gccttgccgg
    20821 cgtactgctc agcctctacc tgcgtgcccg tctcaagttc gcgctggcgc gcaaccgcag
    20881 cgagctcaac cgctgccgca aggagcttga cggtctgcgc gagcagctcg aaagcaaagc
    20941 ccgctcctga ccggcagacc tacacggtgc cttgcgcctt cagtgcggca atccgctgcg
    21001 ggtcgtagcc cagtcgttcg agaatgctgg cggtatgctc gcccagctgc gggccgatcc
    21061 actcggtccc gccgggcgtc tccgacagtt tcggcacgat gccgggcatc ctgaattcct
    21121 tgccgtccgg caggcgcgcg cgctgcagca tgtcgcgggc gagaaattgc ggatcggcga
    21181 acatgtcctc ggcgctgtag atgcggctgg cgggaacgtc ggcctgtctg agcgcggcca
    21241 gcacctcgtc cagtggcagc gactgcaccc agcggtcgat caccccgtac agctcgtcgc
    21301 gccgtgcatc gcgcccggcg ttgtcggcca ggctcgggtc gtcggccagg tcgacgcggc
    21361 cgatggccag catgaagcgt ctgaagatgg catcgccgtt ggcgccgatc tgcacgtgct
    21421 tgccgtccgc actgctatgg atcgaggagg gggtgatgcc gggcatgatg ttgccggtgc
    21481 gctcgcggat gaagccgtag acgtcgaatt ccggcaccag gctttccatc atggcgaaca
    21541 ccgcctcgta cagcgccacg tccaccacct ggcccaggcc gccgttgacc tcgcggtggc
    21601 gcagcgccat cagcgcgccg atcacccccc acagcgcggc gatcgagtcg ccgatggaga
    21661 tgccggtgcg caccggcggg cggtcctcga agccggtgat gtagcgcagc ccgcccatcg
    21721 actcgcccac cgcgccgaag cccggctggt ccttcagcgg cccggtctgg ccgaagccgg
    21781 acaggcgcac catcaccagt ttcgggttga gcgcgtgcag cacgtcccag ccgaggccga
    21841 gcttctccag cacgccgggg cggaagttct cgatcaggat gtcggcctcc tgcatcaggc
    21901 gcttgaggat ggcctgcgcc tcgggatgct tgaggttgag ggtcagcgac tgcttgttgc
    21961 gcgcctgcac gaaccaccat agcgaggtgc cctcgtacag cttgcgccac ttgcgcagcg
    22021 gatcgccgcc atcgggggat tcgaccttga tcacctcggc gccgaactcc gcgcagatgc
    22081 gcgaggcgaa ggggccggcg atcagggtgc cgagttcgat gaccttgagg ccggcgaggg
    22141 gtttggcagg gctggtcatg gatgctccgg ggcgggtggg cgtggctgcg ccagagtgta
    22201 cctccatccg tgggtgcggc agcgtgggtg atatttccgg gtagtggcac ttcgcctctg
    22261 gctccagggg cggtgcgcac gtagagtgga gttttgcagc ccgcgctccg gccggccgct
    22321 ctgccaatgg ccacggcatc tgcccatgac cctgcatcta ccgaccctgc tactgctcga
    22381 catctgcgtg ctcgccctgc tgggcgggct gatgctgcat acctggcggc gcagcgagaa
    22441 cgaggcgact ctcggctgga tggccggcat gctcctgctg gcggcaccgg gcaccctgtt
    22501 gctcagcctg cgcggcatcg gcgtggatac cctgtccatc gtcctcggca atgtgctgct
    22561 gcgcctggcg gctggcctgg gctgggcggc catgcgcgtg ttcgtcggcc gccagccctg
    22621 ctggcccggt gtcgccggcg gtgcgctgct gtgggcgctg ctgtgcctgt ggccgtactt
    22681 catgagttcg ctggcgttgc gggtaacggt cggtaccctg ctcaccatcc tgtatgccgg
    22741 cctggcggca tgggaactgt ggcgcgcgcg ccggcagctg gaggtggcca tcgcgccggc
    22801 cgtggcgctg ctgctgatgc atgtgctgtt ctgcacgggc ctgctgctgc tgggcagcgg
    22861 ccaggccatc gagcgggtgt ggagcgggct ggacgacagc ttcgtgacct ggcgcttgct
    22921 ggagaccttc ctgttcgcca tcggcattgc cttcgtcacc ctggccatgg tgcgcgagcg
    22981 ggccgagctg cgcttgcgcg ccgtggccaa ctgcgatccg ctgaccggca tcggcaatcg
    23041 ccgggccttc atggccgagg cgcaggtgct gctggcgtac tgccagcgca gtgggcgtcc
    23101 ggcggccctg ctgatgtgcg atctcgatca cttcaagcag ctgaacgatt gccatggcca
    23161 cgccatgggc gatacggcgc tgatcggctt cggtcgcgtg ctgagccaga gtatccgcca
    23221 gtccgatgtc tgcgggcgca tcggcggcga ggagtttgcc tgcctgctgc ccgatgccga
    23281 cgccgcgcag gccgagcggg tggccgagcg catccgccgg gagtgccgcg agctgatgcc
    23341 cgatccggcg ctgcgcttca gcgtcagcat cggcgtcgcc agtgtcgtgc aggccggcta
    23401 cgagttgccc cgcttgctgg cgcaggccga cgaggcgctg taccgggcca aggccagcgg
    23461 acgcaaccgg gtcgagcatt acgccgggca gccgatgccg gcctagcggt gcaggcctgc
    23521 gggtgtcgat tcggagtaca ctggcgacct tttccgatca ccaagaaccc gtccatggcc
    23581 ctgcagtcca cgccctacaa agtcgaactc aacctcaccg atctggatcg cagcgtctac
    23641 gagaacctgc gcttcaccgt cgccaagcat ccctcggaaa ccgaggagcg catggcggcg
    23701 cgtttgattg cctatgtgct gtggtaccac gagcaactgt cgttcggccg tggtctgtcg
    23761 gatgtcgacg agccggcgtt gtgggagaag agtctggatg gccgcgtgct gcactggatc
    23821 gaagtcggcc agccggacgc cgagcgcatc acctggtgct cgcgccgctg cgagcgtttc
    23881 agcctggtgg cctacggcaa cctgcgcgtc tggcagacca aggtgctcga tggcgtgcgc
    23941 agcctgaaga acatcaacgt ggtggctgtc gatcaggctg ccttggagaa cctggccaag
    24001 gacatgccgc gcgcgatcag ctggagcgtg atgatcagcg acggttcgct gttcatcacc
    24061 gacgagcgcg gccagcacga agtcccgctc gaatggctcg ccggcgagcg ctgagagagc
    24121 cgtcgcgcag gggcgaatga ttcgcctcgg gaacccgaac ggcgaataaa ttcgccccta
    24181 ccgtcaagct ccggaacgtc atgcgtatcg aaccccgccc gttgcccgcc gagttgcccg
    24241 acctgggcga tctgcccccc ctactgaccc gcctgtacgc cgcccgcggc gtgcagtccg
    24301 ccgccgagct ggacaagacg ctggcgcggc tgatcccgta tcgcgagctg aagggcatcg
    24361 acgcggcggt cgaactgctg gtgaccgcgc ttgagcaggg ccagcgcatc gtcatcgtcg
    24421 gcgacttcga tgccgacggc gccaccgcca gctcggtcgg cgtgctggcg ctgcgcatgc
    24481 tgggtgccgc gcatgtcgac tatctggtgc ccaaccgctt cgagtacggc tacggcctga
    24541 ccccggagat cgtcgcggtg gcgctggaca agcggccgca actgctgatc accgtggaca
    24601 acggcatttc cagcctcgac ggcgtggccg cggccaaggc ggcgggcctc gcggtgctgg
    24661 tcaccgacca ccacctgccc ggtcccgagc tgccggcggc ggatgccatc gtcaacccca
    24721 accagccggg ctgcccgttc ccgagcaagg cgctggccgg ggtcggggtg atcttttacg
    24781 tgatgctggc cctgcgcgcg cggttgcgcg agctggactg gttcgcccgc cgcggcctgg
    24841 ccgagccgaa cctggccgag ctgctcgacc tggtggcgct gggcagcgtc gccgacgtgg
    24901 tgccgctgga cgccaacaac cgcatcctgg tccatcaggg cctggcgcgc attcgtgccg
    24961 gtcgggcgcg gccggggttg cgtgcgg
//
LOCUS       DATCUN010000240         5063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_161012, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000240 DATCUN010000000
VERSION     DATCUN010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161012"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..594
                     /gene="zur"
                     /locus_tag="VIO83_09225"
     CDS             109..594
                     /gene="zur"
                     /locus_tag="VIO83_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HEY8331493.1"
                     /translation="MSKTPLACTPHDHDHCVSHALAEADALCARLGVRLTALRRRVLE
                     LVWQSHRPLGAYDILAVLSEQDGRRAAPPTVYRALDFLLENGLVHRIASLNAFIGCIH
                     PEHPHQGQFLICRSCHTAIELEQPSIAEAIEAAARNVGFAVEGQTVEVVGLCTPCRSA
                     A"
     gene            591..1367
                     /gene="znuC"
                     /locus_tag="VIO83_09230"
     CDS             591..1367
                     /gene="znuC"
                     /locus_tag="VIO83_09230"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HEY8331494.1"
                     /translation="MSDTLLRLDDIHVRFAGQDVLQGAHLQVHRGEIVTLIGPNGAGK
                     TTLVRVVLGLLKPERGEVWRKPKLRVGYMPQKLHVDATLPLSVLRFLRLVPGVDRARA
                     LTALSEVGAEQVIDSPLQKISGGELQRVLLARALLRQPELLVLDEPVQGVDVAGQAEL
                     YRLIGRLRQRYGCGVLMVSHDLHLVMSATDRVICLNRHVCCSGHPDKVSGDPAFRELF
                     GNDARALAIYHHQHDHAHDLHGGVVLAKKPHIHGPNCNHG"
     gene            1360..2148
                     /gene="znuB"
                     /locus_tag="VIO83_09235"
     CDS             1360..2148
                     /gene="znuB"
                     /locus_tag="VIO83_09235"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HEY8331495.1"
                     /translation="MADFLLNALLAGLALALVAGPLGSFVVWRRMAYFGDTLAHAALL
                     GVALGFLLDVSPTLAVTVGCIILAVLLVTLQQRQPLAADTLLGILAHSTLSLGLVTIS
                     FLDKVRVDLMAYLFGDLLAVGSGDLLWIIGGSLLVLAVLAWLWRPLLALTVHEELARV
                     EGLPVGALRLALMLLIAVVIAVAMKIVGVLLITSLLIIPAASAQRHARSPEQMAAGAS
                     LVGLVAVCAGLALSWYQDTPAGPSIVVSAAGLFLLSFLLPRRAA"
     gene            2636..4198
                     /gene="tssA"
                     /locus_tag="VIO83_09240"
     CDS             2636..4198
                     /gene="tssA"
                     /locus_tag="VIO83_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010565818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HEY8331496.1"
                     /translation="MTYSSKLAAHYMEVACTPISPDDFSGVDVRYSTAFEQLENELGK
                     SASLHETAGIDWALVRSGSEAVLREQSKDLRVAAWLTWSLYQQDSFTGLHAGLSLLHH
                     LCLQHWDELHPRKPRTRAAAIGWLVPRLEQALAEDVPLGEQLPLFRALAERLRALEAC
                     LSEQLGDAAPLLLPFCRRLDELVERAAQGRPEPGKVGAALAQVKQVATQILSANSPVD
                     NDKDAQKALRSQQEAARPLCAWWLKQKASDLRALRLSRTLLWLPIDSLPERNAEQVTP
                     LRGLPADKLAGFRERFAQGLYADLLVDLEASMARAPFWLDGQRLAWECLQELDAAQAM
                     GEVEIQLALFLQRVPGIEELRFHDGAPFADADTRAWIASHVLPHLQLPQAGQQAVPVD
                     AGALPAWEDALQQVLPQLRADGLKPAVQQLKRGLAQARGGRERFFWQLSLVRLCLAAK
                     KYELAKTQLESLDQTLQAAGLGDWEPDLALEVLRLLHSCCELLPQSHVVRERKEEIYR
                     RLCHLDLEVVLE"
     gene            4217..4723
                     /gene="tssB"
                     /locus_tag="VIO83_09245"
     CDS             4217..4723
                     /gene="tssB"
                     /locus_tag="VIO83_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HEY8331497.1"
                     /translation="MAKEGTVAPKERINVTFKPATGGAQEEIELPLKMMVLGDFTQRS
                     DDRKIEDRKPIAIDKHSFDDVLAKQELSLTLAVPNRLQDEPGDEELAVRLRIESMKDF
                     NPANLVEQVPELQKLMELRDALVALKGPLGNAPAFRKAIESVLADDDSRERVLAELGL
                     AAQDKLDS"
     gene            4749..>5063
                     /locus_tag="VIO83_09250"
     CDS             4749..>5063
                     /locus_tag="VIO83_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEY8331498.1"
                     /translation="MSTRAAVASSRHTADAGILDRIIAETKLTPDDEAYSIAKRGVSA
                     FIEELLKPQNEHEPVKKAMVDRMIAEIDAKLSRQMDEILHHPQFQSLESSWRGLKLLV
                     DRT"
BASE COUNT          864 a         1809 c         1610 g          780 t
ORIGIN      
        1 caagcaacgc ggcaagcagc gggagagaga aaagacgctt cacgggttat cactcaggag
       61 gctggaaatg gtacataata acgtctctcc atcagaacgt cgccgcccat gtccaagact
      121 ccgctggcct gcacgcccca cgaccacgac cactgcgtca gccatgcgct ggccgaagcc
      181 gatgcgctct gcgcgcgcct gggcgtgcgc ctaaccgccc tgcgccggcg cgtgctggag
      241 ctggtctggc agagccaccg gccgctcggc gcctacgaca ttctcgccgt attgagcgag
      301 caggacggcc gccgcgccgc cccgccgacc gtgtaccgcg cgctggattt cctcctggag
      361 aacggcctgg tgcaccgcat cgcctcgctc aacgccttca tcggctgcat ccatccggag
      421 catccgcacc agggccagtt cctcatctgc cgcagctgcc acaccgccat cgagctggag
      481 caaccgtcca tcgccgaggc catcgaggcc gccgcgcgca acgtcggttt cgccgtcgaa
      541 ggccagaccg tcgaagtggt cgggctgtgc acgccgtgcc ggagtgcggc atgagcgata
      601 cgctgctgcg actggacgac atccatgtgc gcttcgccgg ccaggacgtg ctgcaaggcg
      661 cgcatctgca ggtgcaccgc ggcgagatcg tcacgctgat cggccccaac ggcgccggca
      721 agaccaccct ggtgcgcgtg gtgctcggcc tgctcaagcc ggagcgcggc gaggtctggc
      781 gcaagccgaa gctgcgggtc ggctacatgc cgcagaagct gcatgtcgat gccacgctgc
      841 cgctgtcggt gctgcgtttc ctgcgtctgg tgccgggcgt ggatcgtgcc cgcgcgctga
      901 cggcgctgag cgaggtgggc gccgaacagg tgatcgacag cccgctgcag aaaatttccg
      961 gcggcgaact gcagcgcgtg ctgctggccc gcgccctgct gcgccagccg gaactgctgg
     1021 tgctcgacga gccggtgcag ggcgtcgacg tcgccggcca ggccgagctg taccgcctga
     1081 tcggccgcct gcgccagcgc tacggctgcg gcgtgctgat ggtctcccac gacctgcatc
     1141 tggtgatgag cgccaccgac cgggtgatct gcctcaaccg ccacgtgtgc tgttccggcc
     1201 acccggacaa ggtcagcggc gacccggcct tccgcgagct gttcggcaac gacgcgcgcg
     1261 ccctggcgat ctaccaccac cagcacgacc acgcccacga cctgcacggc ggcgtggtgc
     1321 tggccaagaa accccacatt cacggcccca actgcaacca tggctgattt cctgctcaac
     1381 gccctgctcg ccggcctcgc cctggcgctg gtcgccggcc cgctcggctc cttcgtggtc
     1441 tggcggcgca tggcctactt cggcgacacc ctcgcccacg ccgccctgct cggcgtggcg
     1501 ctgggcttcc tgctcgacgt cagcccgacc ctggcggtga ccgtcggctg catcatcctc
     1561 gccgtgctgc tggtcaccct gcagcagcgc cagccgttgg ccgccgacac cctgctcggc
     1621 atcctcgccc acagcaccct gtcgctgggc ctggtcacca tcagcttcct cgacaaggtg
     1681 cgcgtcgacc tgatggccta cctgttcggc gacctgctgg cggtggggtc gggcgacctg
     1741 ctgtggatca tcggcggcag cctgctggtg ctcgccgtgc tcgcctggct gtggcgcccg
     1801 ctgctggcgc tcaccgtgca cgaggagctg gccagggtcg agggcctgcc ggtcggcgcc
     1861 ctgcgcctgg cgctgatgct gctgatcgcc gtggtgatcg ccgtggcgat gaagatcgtc
     1921 ggcgtgctgc tgatcacctc gctgctgatc atccccgccg ccagcgccca gcgccatgcg
     1981 cgcagccccg agcagatggc cgccggcgcc agcctggtcg gcctggtcgc ggtgtgcgcc
     2041 ggcctggccc tgtcctggta ccaggacacc ccggccgggc cgtccatcgt ggtcagcgcc
     2101 gccgggctgt tcctgctgag cttcctgctg ccgcgccggg ccgcctgagg ccctgcgcat
     2161 tgccccgccc aggcccgccc tgggtggggc gccttcctga cggccgacgg ccgcacacga
     2221 ccgcgcaacc aagcgtcggc catctgcgca acctcgccac tcccgctctg caagccagac
     2281 gccacatatc aaaagcatat ccaactgaca tcaccgcaat atcacccgca ctcgccaaac
     2341 ctgcagccga atatccataa tccggaatat cgcagtaaat tccccggaca atatggaaac
     2401 cattcacacg caatgtgtgt cgccactccc atattcccat acgggaaaca aggtttattc
     2461 cccggcacat tgttaaaccg ccaacagcgc tctaggatgc ccgccggaca agggatgtgc
     2521 gcagctttct gccaccacaa gtgcgcaaga agttgcacag caaagatatc aacaggcgcc
     2581 cgaaatgctt ttcgggataa cttgaagaat atcttcttat atattgagtc aacggatgac
     2641 ctattccagc aagctggctg cccattatat ggaagtggcc tgcacgccaa ttagcccgga
     2701 cgacttttcc ggcgtcgacg tgcgttattc gaccgccttc gagcagcttg aaaacgagct
     2761 gggcaagagc gcgtccctgc acgaaaccgc cggcatcgac tgggcgctgg tgcgctccgg
     2821 cagcgaggcc gtgctgcgcg agcagtccaa ggatctgcgg gtcgccgcct ggctgacctg
     2881 gagcctctac cagcaggact ccttcaccgg cctgcacgcc gggctaagcc tgctgcatca
     2941 cctctgcctg cagcactggg acgaactgca tccgcgcaag ccacgcaccc gtgcggccgc
     3001 catcggctgg ctggtgccgc gcctggaaca ggcactggcc gaggacgtgc cgctcggcga
     3061 gcagctgccg ctgttccgcg ccctcgccga acggctcaga gcgctcgaag cctgcctgag
     3121 cgaacagctg ggcgacgccg ccccgctgct gttgccgttc tgccggcgcc tcgacgagct
     3181 ggtcgaacgg gccgcccaag gccgcccgga acccggcaag gtcggcgcgg cgctggccca
     3241 ggtcaagcag gtcgccaccc agatcctcag cgccaacagc cccgtcgaca acgacaagga
     3301 tgcccagaag gcgctgcgca gccagcagga ggccgcccgc ccgctctgcg cctggtggct
     3361 caagcagaag gccagcgacc tgcgcgccct gcgcctgagc cgtaccctgc tgtggctgcc
     3421 gatcgacagc ctgccggagc gcaatgccga gcaggtcacc ccgctgcgcg gcctgccggc
     3481 ggacaagctg gcggggtttc gcgaacgctt tgcccagggc ctgtacgccg acctgctggt
     3541 cgacctcgag gccagcatgg cccgcgcgcc gttctggctc gacggccagc gcctggcctg
     3601 ggaatgcctg caggagctgg atgccgccca ggccatgggc gaggtggaga tccagctggc
     3661 gctgttcctg caacgggttc ccggcatcga ggagctgcgc tttcacgatg gcgcgccgtt
     3721 cgccgatgcg gatacccgcg cctggatcgc cagccatgtg ctgccgcacc tgcagctgcc
     3781 gcaggccggg cagcaggcgg tgccggtgga cgccggagcg ctgccggcct gggaggacgc
     3841 cctgcaacag gtgctgccgc agctgcgcgc ggacggcctg aagcccgccg tgcagcagct
     3901 caaacggggc ctggcccagg ctcgcggcgg acgggaacgc ttcttctggc aactgagcct
     3961 ggtccgcctg tgcctggccg ccaagaaata cgaactggcc aagacccaac tcgaatcgct
     4021 cgaccagacg ctgcaggccg ccggcctggg cgactgggag cccgatctcg ccctagaagt
     4081 gctgcgactt ctgcacagct gctgcgaact gctgccgcag agccacgtcg tgcgtgaacg
     4141 caaggaagag atctaccgca ggttgtgcca cctcgacctc gaggtggtac tggaatgacc
     4201 gacatggaga acccacatgg ccaaagaagg cacggtagcc cccaaggaac gcatcaacgt
     4261 caccttcaag cccgccaccg gcggtgccca ggaagagatc gagctgccat tgaagatgat
     4321 ggtgctcggt gatttcaccc agcgcagcga cgaccgcaag atcgaggacc gcaagccgat
     4381 cgccatcgac aagcacagct tcgacgacgt gctggccaag caggagctga gcctgaccct
     4441 cgccgtgccc aaccgcctgc aggacgagcc gggcgacgag gagctggccg taaggctgcg
     4501 catcgagtcg atgaaggact tcaacccggc caacctggtc gagcaggtgc cggagctgca
     4561 gaagctgatg gagctgcgcg atgccctggt cgccctgaag ggtccgctgg gcaatgcccc
     4621 ggccttccgc aaggccatcg agagcgtgct ggccgacgac gactcgcgcg agcgcgtgct
     4681 cgccgagctg ggcctggccg cccaggacaa gctggattcc tgacctcaac cgacaaggac
     4741 gctgattgat gagtacccgt gcagcagtag caagcagtcg ccacaccgcc gatgccggca
     4801 ttctcgaccg catcatcgcc gagaccaagc tgaccccgga tgacgaggcc tactcgatcg
     4861 ccaagcgcgg cgtgtcggcc ttcatcgagg agctgctcaa gccgcagaac gaacacgagc
     4921 cagtgaagaa ggccatggtc gaccgcatga tcgcggagat cgacgccaag ctcagccggc
     4981 agatggacga gatcctccac cacccgcagt tccagtcgct ggagtcgtcc tggcgcggcc
     5041 tcaagctgct ggtcgaccgc acc
//
LOCUS       DATCUN010000241         4330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_236078, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000241 DATCUN010000000
VERSION     DATCUN010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236078"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VIO83_09255"
     CDS             <1..486
                     /locus_tag="VIO83_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY8331499.1"
                     /translation="GAGLHACIMAALAAQSGSALAGGCELVEELLHGIETEKRLKDRV
                     READQRRQRLPGFGLPLYPDGDPRARYLIQLALTIAPASRQVELAAKFVDQVREKVGI
                     HANIEVGLATLTLALGLPRRASGALWVVGRTAGWIAHVLEQRRAGHMLRARGHYVAQA
                     Y"
     gene            complement(751..1266)
                     /locus_tag="VIO83_09260"
     CDS             complement(751..1266)
                     /locus_tag="VIO83_09260"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY8331500.1"
                     /translation="MGQALWVRVPANEPGMIGKVLDLGFKGLIRPMADSVDDARRLVA
                     ATRFLSPGERSYGQTRALLYERPTGFVERSNLDVLVLAMIETREALRPRERDMFRRRL
                     DGVYIGPNDLGISMGHGAAIDREDGEVLAAFDHILAAARARRKFAGIHTASVDHAARM
                     LARGLRHGDGV"
     gene            1490..2152
                     /locus_tag="VIO83_09265"
     CDS             1490..2152
                     /locus_tag="VIO83_09265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8331501.1"
                     /translation="MRPPATRFGMFLDDYATILNPRRYVEAMTAAFVGAGGEIERAGV
                     SALTREGDGWRLQGAGSRRFEHVVVAAGAWSRTLLDPLGIRVPLESQRGYHVQFEGGD
                     GVISRTVVLAGKKVFATPMELGLRFGGTVEIGGLARPPDARRSDVLQRIAVESFPQLA
                     GLSAQTWMGHRPCMPNSVPVISEAQGQPGLWLAIGHDHLGLTDSLPTALRISDGICGA
                     RS"
     gene            2266..3003
                     /locus_tag="VIO83_09270"
     CDS             2266..3003
                     /locus_tag="VIO83_09270"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8331502.1"
                     /translation="MDNAVNGSTVNQFGQPIGDPMPGWSPRPAPADVTLQGRFCRLEP
                     LDAERHAEDLFAAYSTAHDDRDFTYLFAGPYRDAGEHRSFAEGAARSADPKPYAVVDT
                     AQGKAVGTLSLMRIDPKNGVIEVGGVTFSRLLSRKPASTEAQFLLMQYAFDGLGYRRY
                     EWKCDSLNAPSRTTALRLGFHFEGIFRQAIVYKGRSRDTAWYSVIDKEWPLLKQAFLA
                     WLAPGNFDADGQQVRSLAAIRDSLSAA"
     gene            complement(3153..4085)
                     /locus_tag="VIO83_09275"
     CDS             complement(3153..4085)
                     /locus_tag="VIO83_09275"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEY8331503.1"
                     /translation="MSNPPFRRLLVVGADSPLGHRVTELLLRIGDVSVTAGSPNPDQL
                     ADLVARGAQICRVDVDDPDSLQTAFGGVQRLLLVADDGAGTAGLRRRQCETVAEAAVG
                     ARLEHVVWVSEASSELGSPLTEDAQEVNPAIEASGIPCTFLRLNRYAESLFARLSLAL
                     RSGWWLSSAGEARVAYVARDDAARAGAAALCARTAVGQRVDITGPAALTSAEVVALTN
                     SIFGSRIELVPMDDGTFSEALAAAGMMQSEAERMLAVERGARAGLEGRVSGAVEYLTG
                     RAPRGLAGVLLEHRMEILLASRSGGRFVSPGLRR"
BASE COUNT          743 a         1489 c         1434 g          664 t
ORIGIN      
        1 ggcgccgggc tgcacgcgtg catcatggcc gcgctggcgg cgcagagcgg cagcgcactg
       61 gccggcggct gcgaactcgt ggaggagctg ctgcacggca tcgagacgga gaagcgcctg
      121 aaagaccggg tacgcgaggc cgatcagcgg cgccagagat tgcccggctt cgggttgcca
      181 ctctaccctg acggcgaccc acgcgccagg tacctgatcc agttggcgct gaccatcgcg
      241 cctgcctcgc ggcaggtcga gctggcggcg aagttcgtgg atcaggtgcg cgagaaggtc
      301 ggcatccacg ccaacatcga ggtgggtctc gccaccctca cgctcgcact gggattgccc
      361 cggcgcgcct ccggcgcgct ctgggtggta ggccgcacgg caggatggat cgcccacgtg
      421 ctggaacagc ggcgcgccgg ccatatgctg cgagcgcgcg gccactacgt agcgcaggcc
      481 tattgacgaa atgcgcctgc ggtgtcctag ggatcgagca gcaccaggcg gccttcatgg
      541 ccggcccgcg cgtggcaccg acccatcgat ggcggatggg ggcaccaccg ccctttggga
      601 tctcgtggcg tcgcggacac agtaggtgcg cgacgaacac ggaccgtgag cggcgctgcg
      661 agcgagccta ttcaggccat ggtgggtccg atgcggtccc ggaagcgcgc gacgccgttc
      721 attgccccct cttaccgaaa actggcgtcg ctagacacca tcgccatgtc gaagcccccg
      781 ggccagcatc cgagcagcat ggtccacgct tgctgtatga attcccgcga acttgcgcct
      841 cgcgcgggcg gcagcaagga tgtggtcgaa cgcggccaag acttccccgt cctcccggtc
      901 gatcgccgcc ccgtggccca tcgagatccc aaggtcgtta ggtccgatgt acacgccatc
      961 cagccgccga cgaaacatat ctcgctcacg tgggcgcaaa gcttcgcgcg tctcgatcat
     1021 cgccagaacc aagacatcga gattggaccg ctccacgaat cccgtgggcc gctcgtacag
     1081 aagtgcgcgc gtctggccat agctgcgttc gcccggcgac agaaagcgcg tcgcagccac
     1141 cagcctgcgc gcgtcatcga ccgagtcggc catcggacgg atgagaccct tgaaaccgag
     1201 atcgaggact tttccgatca tccccggctc gttggccggc acacgaaccc agagggcttg
     1261 ccccatgcgg ctgcatcccc tggatcgcat ggatggcgga cgagtagtcg agcagcccgt
     1321 tctgaaggtc gaccatgacg ctgtcgaagc cgccgcgcgc catgcacttg gcaacgaagg
     1381 atgacgcgat ggtgatccat caattgaggg ttctggtggc aactccgggc gcagtagggt
     1441 tacgtcgcgc agtgcctcga ccgtgccggg atcgagcagc tcgaacctaa tgcgcccgcc
     1501 cgctaccagg ttcggcatgt tcctagacga ctacgcgacc atcctgaatc cacgccgcta
     1561 cgtggaagcg atgaccgcgg ccttcgtcgg tgcgggcggc gaaatcgaac gcgccggtgt
     1621 gtcggcgctg acgcgcgaag gcgacggctg gcggctgcag ggtgctggct cccgccgctt
     1681 cgagcacgtc gtcgtcgccg cgggtgcgtg gtcgcgcacg ctgctggacc cgctgggcat
     1741 cagggtgccg ctggagagcc agcgcggcta ccacgtccag ttcgaagggg gcgatggcgt
     1801 catctcgcgc acggtcgtgc tcgccggcaa gaaagtgttc gcgacgccca tggaattggg
     1861 gctgcgcttt ggcggcacgg tcgagatcgg agggctggcg cggccgcccg acgcgcgccg
     1921 ctccgacgtg ctgcagcgca ttgccgtcga gagctttccg cagttggcgg gtctgtcggc
     1981 gcagacgtgg atgggccacc gcccctgcat gcccaactcc gtgcccgtca tcagcgaggc
     2041 gcagggccag ccggggctgt ggctggcgat cgggcacgac cacctcggcc tgacggattc
     2101 cctgccaacg gcgctgcgga tctccgacgg gatttgtggc gcccggtcct gacacaggcg
     2161 gtcagcacgg ggcatggcgc cgcaacggca ggatcggtgg ccccgtatcg ccacagcatg
     2221 atggagggtg cacgcaggcg cacctgtatc ttttggagga gacggatgga caacgcagtc
     2281 aacggcagca cagtgaatca gttcggacaa ccgatcggcg accccatgcc gggttggtcg
     2341 ccccgtcccg cgccagcgga tgtgaccttg cagggcaggt tctgccgcct ggagccgctg
     2401 gatgccgaac ggcatgccga ggacctgttc gcggcctaca gcaccgcaca cgacgaccgc
     2461 gatttcacct acctgttcgc aggcccctac cgcgatgctg gcgagcaccg cagctttgcg
     2521 gaaggtgccg cacgcagcgc cgatcccaag ccctacgcgg tcgtggacac ggcgcagggc
     2581 aaggcggtcg ggacgctctc gctcatgcgg atcgacccca agaacggcgt gatcgaggtg
     2641 ggaggggtca ccttttcgcg gctgctcagc cgcaagccgg cgtcgacgga ggcgcagttc
     2701 ctgttgatgc agtacgcctt cgacgggctc ggataccggc gctacgagtg gaagtgcgac
     2761 agcctgaacg caccgtcgcg cacgacggcg ctgcggctgg gctttcattt cgagggcatc
     2821 ttccgccagg ccatcgtcta caagggccgc tcgcgcgaca cggcatggta ttccgtaatc
     2881 gacaaggaat ggccgctgct caagcaggcg ttcctcgcct ggctggcccc ggggaacttc
     2941 gatgccgacg gtcaacaggt ccgctcgctc gccgcgattc gggacagctt gtcggccgcc
     3001 tgagcgggcc ggctgcccgt acgcgggacg cagcgggcct cggcacaggc ctcagcgcgc
     3061 gctcctcgcc ccccgaaggc gaccgcaggc cacagcctga gcctcttcgg ctggggaggg
     3121 cctggtggca cgaccgccgc gggcacctgc agtcagcgac ggagaccggg gctgacgaac
     3181 cgaccgccgc tgcgactggc aagcaggatc tccatgcggt gctcgagcag gacccccgcg
     3241 agtcctcgcg gggcccggcc cgtcagatac tcgaccgcgc cgctgacccg gccttccagc
     3301 cccgcccggg cgccacgctc aaccgccagc atccgctccg cctcggactg catcatgccc
     3361 gcagccgcga gcgcctcgga gaacgtcccg tcgtccatcg ggaccagttc gatccgggag
     3421 ccgaaaatgc tgttggtgag ggcgaccacc tccgccgacg tcagtgccgc cggccccgtg
     3481 atatcgacac gctggcccac cgccgtgcgc gcgcacaacg cagcggctcc agctctggcc
     3541 gcatcatcgc gcgccacata ggccaccctc gcctccccgg ccgaactcaa ccaccaaccg
     3601 gagcgcagcg ccagggacag ccttgcgaac agcgactcgg cataccggtt caaacgcaag
     3661 aaggtgcagg gaatgccgct ggcctcgatg gccggattca cctcctgcgc atcctcagtc
     3721 aggggcgagc cgagttcgga acttgcctct gacacccaaa cgacgtgttc gagcctcgca
     3781 ccgaccgcag cttccgccac ggtctcgcac tgccggcggc gcaggcctgc ggtgccggct
     3841 ccgtcgtcgg cgacgagcag cagccgctgc acaccgccga acgccgtctg gagcgaatcc
     3901 ggatcgtcga cgtcgacgcg gcatatctgt gcgccgcggg ccaccagatc tgcaagctgg
     3961 tcagggttgg ggctgccggc cgtcaccgaa acatctccta tgcgcaatag cagctcggtc
     4021 acgcgatggc ccagcgggct gtcggcaccg actacgagca gacgacgaaa aggtggattc
     4081 gacatggcaa gccccttgtg acgaccgcga agaccgtcga tgcacgacgc acccccggcc
     4141 accgcagggc gattgtattg acaaaaaccg actcaaagtc gaataatcac cttaaggcta
     4201 cgctgaacaa gccgtcgaaa atttccgcgc aaacctggat gacgcagttt tttggacttt
     4261 tatgcctctc gcgcggccca tttgggcagt tcgcggggcg ctgaggcccg atttccgccc
     4321 ttacgggcgc
//
LOCUS       DATCUN010000242         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_161032, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000242 DATCUN010000000
VERSION     DATCUN010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161032"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /gene="ubiB"
                     /locus_tag="VIO83_09280"
     CDS             complement(<1..1334)
                     /gene="ubiB"
                     /locus_tag="VIO83_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEY8331504.1"
                     /translation="MKLLALRRLIHIQRTAVRYQLDELLLDLPLPWWLRSLLRAMPWR
                     WLPRKPIEMSRGERLRRSLEDLGPVFIKFGQLLSTRRDLLPPDIADELAHLQDKVPPF
                     PPEQAVALIEQQLGASIDQVFSRFERQPLASASVAQVHAAQLRTGEDVVVKVVRPGLE
                     PVIRQDMAWLFVLAKLAERTSADARRLRPVEVVKDYEGTIFDELDLLREAANGSQLRR
                     NFEGSPLLYVPQVYWHWCRPKVLVMERIYGVPVTDLAALHDQHTDMKKLAERGVEIFF
                     TQVFRDSFFHADMHPGNIFVSTRTPHDPQYIAIDCGIVGSLTAEDQDYLARNLLAFFK
                     RDYRKVAQLHIDSGWVPADTKVNDFEAAIRTVCEPIFEKPLKDISFGQVLLRLFQTAR
                     RFNMQVQPQLVLLQKTLLNIEGLGRQLYPELDLWSTAKPFLERWMRERMSPLR"
     gene            complement(1339..>1530)
                     /locus_tag="VIO83_09285"
     CDS             complement(1339..>1530)
                     /locus_tag="VIO83_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020293518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEY8331505.1"
                     /translation="QWGSDSLASLRLNLADWLAEESRALLGRREAEARFAELDHLKLA
                     LDRLEARSERLARRLPEAD"
BASE COUNT          232 a          489 c          568 g          241 t
ORIGIN      
        1 cgcagcgggc tcatgcgctc gcgcatccag cgctcgagga acggcttggc ggtgctccac
       61 aggtcaagct cggggtacag ctggcggccg aggccctcga tgttgagcag ggtcttctgc
      121 agcagcacca gctgcggctg cacctgcatg ttgaagcgcc gcgcggtctg gaacaggcgc
      181 agcagcacct ggccgaagga gatgtctttc agcggcttct caaagatcgg ctcacagacg
      241 gtgcggatcg ccgcctcgaa gtcgttgacc ttagtgtcgg cgggcaccca gccggagtcg
      301 atgtgcagct gcgccacctt gcggtagtcg cgcttgaaga aggccagcag gttgcgcgcc
      361 aggtagtcct ggtcctcggc ggtgaggctg ccgacgatgc cgcagtcgat ggcgatgtat
      421 tgcgggtcgt gcggcgtgcg ggtgctgacg aagatgttgc cggggtgcat gtcggcgtgg
      481 aagaagctgt cgcggaacac ctgggtgaag aagatctcca cgccgcgctc ggcgagcttc
      541 ttcatgtcgg tgtgctggtc gtgcagggcg gccaggtcgg tgaccggcac gccgtagatg
      601 cgctccatta ccagcacctt gggccggcac cagtgccagt acacttgcgg cacgtagagc
      661 agcggtgagc cttcgaagtt gcgccgcagc tggctgccgt tggcggcttc gcgcagcagg
      721 tcgagctcgt cgaagatggt gccctcgtag tccttcacca cctccaccgg gcgcaggcgg
      781 cgggcgtcgg ccgaggtgcg ctcggccagc ttggcgagca cgaacaacca ggccatgtcc
      841 tggcggatca ccggttcgag gcccgggcgc accaccttga ccaccacgtc ctcgcccgtg
      901 cgcagctggg cggcgtgcac ctgggccacc gaggcggagg ccagcggctg gcgctcgaag
      961 cggctgaaca cctggtcgat cgacgcgccg agctgctgct cgatcagcgc caccgcctgc
     1021 tccggcggga acggcggcac cttgtcctgc aggtgcgcca gctcgtcggc gatgtccggc
     1081 ggcagcaggt cgcggcgggt ggacagcagc tggccgaact tgatgaacac cgggccgagg
     1141 tcttccaggc tgcggcgcag gcgctcgccg cgcgacatct cgatgggttt gcgcggcagc
     1201 cagcgccagg gcatggcgcg cagcaggctg cgcagccacc agggcagcgg caggtcgagc
     1261 agcagctcgt cgagctggta acggacagcg gtgcgctgga tatggatcag gcggcgcagg
     1321 gcgagcagct tcatgcggtc agtcggcttc cggaaggcgg cgggcaaggc gctcgctgcg
     1381 cgcctcgaga cggtcgaggg cgagcttgag gtggtccagc tcggcgaagc gcgcttccgc
     1441 ctcgcggcgg ccgagcagcg cgcgcgactc ttcggccagc cagtcggcca ggttcaggcg
     1501 caggctggcc aggctgtcgc tgccccactg
//
LOCUS       DATCUN010000243         5065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_274003, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000243 DATCUN010000000
VERSION     DATCUN010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274003"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VIO83_09290"
     CDS             complement(<1..256)
                     /locus_tag="VIO83_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY8331506.1"
                     /translation="MKAKHLRADVLAGLTTSFALVPECIAFALVAQLNPLMGLYGAFI
                     ICTLTAMFGGRPGMVSGAAGSMAVVIVALVVQHGAQYLLAT"
     gene            600..2378
                     /locus_tag="VIO83_09295"
     CDS             600..2378
                     /locus_tag="VIO83_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8331507.1"
                     /translation="MADYKAPLRDMDFVLNEVFEVSKQWADLPALAEVVDADTAKAIL
                     EEAGKVSAGVLAPLNRSGDEEGCSWHEGAVQTPAGFPAAYQAYGEGGWVGVGGSPEFG
                     GMGMPKVIGAQVEEMINSANLSFALYPMLTAGACLALLNHGSEELKAQYLPAMYEGRW
                     TGSMCLTESHAGTDLGMIRTKAEPQADGSYKVSGTKIFITGGEQDLSENIIHLVLAKL
                     PDAPAGAKGISLFLVPKVLVNADGSLGARNAVSCGSIEHKMGIKASSTCVMNFDGATG
                     YLVGELNKGLNAMFTMMNYERLGVGIQGLALGERSYQSAVEYARERIQSRAPTGAVAR
                     DKAADPIIVHPDVRRMLLTMKALNEGGRAFSSFVALQLDTAKYSEDAVARKRAEELVA
                     LLTPVAKAFLTDMGLETTIHGQQVFGGHGYIREWGQEQLVRDCRITQIYEGTNGIQSL
                     DLMGRKVVASGGSYYRHLSEEINAFIAGADAALGEFTGPLKAAVENLDELTAWVLDKA
                     QNNPNEIGAASVEYLHAFGYTVYAYLWARMAAVARAKQGEGDFYASKLGTARFYFARI
                     LPRIHSLSASIKAGSASLYLLDAEQF"
     gene            2568..3440
                     /locus_tag="VIO83_09300"
     CDS             2568..3440
                     /locus_tag="VIO83_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020298620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEY8331508.1"
                     /translation="MSDLVRMGVLLGALALPAAVVAAENSPPVAGSSQYAQEEQAARA
                     MLAKAVAYYRQEGEKALATFSRRGPFTVDNLYVYVVDAQGVLVASGGPSILLAGQDIG
                     EAVGAEARAKLMSREGGEGHVGEIEYPFRDWTLDGKVVKKHTFYQWVGDHVIAVGYYM
                     PRADSADARKLLDEAAKAVASEPEATLDAINKFDKRYYQGDLYVLVVDLQTKRFVAHG
                     YNRRLLARDFASIKGPDGKLIGQPMLDIALNKGEGEFDYQWLNPVTKQNEPKHAYIRR
                     VGNYLVAVGYYQPQ"
     gene            complement(3562..3827)
                     /locus_tag="VIO83_09305"
                     /pseudo
     CDS             complement(3562..3827)
                     /locus_tag="VIO83_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248887.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
     gene            4083..4511
                     /locus_tag="VIO83_09310"
     CDS             4083..4511
                     /locus_tag="VIO83_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331509.1"
                     /translation="MNRKAWLALMVAAVASQSYGMTDERGLVSIELKASPQNTAEIGR
                     ATLVTEGSRTAIVFALSGAPSHVALPLHYYTYIYPGSCNALGAEPAYDMNAVVRVESF
                     GHLSKRIPVALEQLRGGNYSIVVRSSPADGRLDLFCAEII"
     gene            complement(4553..>5065)
                     /locus_tag="VIO83_09315"
     CDS             complement(4553..>5065)
                     /locus_tag="VIO83_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY8331510.1"
                     /translation="DELFCSRNFGPAIHKFGAILGGAFNFVDQNLFGGKIPLTLHDNK
                     PDYACLKPAAEAQKIDYPKPDGKLSFDKLSSVFLSNTNHEEDQPCHLKLADASIPLGK
                     NLPLYDEPAQRYCPAGVYEIVTLESGEKKFQINAQNCVHCKTCDIKDPAQNITWVAPE
                     GTGGPTYPNM"
BASE COUNT          962 a         1598 c         1655 g          850 t
ORIGIN      
        1 cggtggcgag caggtactgc gcgccgtgtt gcaccaccag cgcgactatc accacggcca
       61 tcgagccggc agcgccggaa accatgcccg gccggccgcc gaacatcgcg gtcagggtgc
      121 agatgatgaa ggcgccgtac aaccccatca gcggattgag ctgggcgacc agggcgaagg
      181 cgatgcactc gggcaccaag gcgaaggatg tggtgagtcc ggccaggaca tcggcacgca
      241 gatgttttgc tttcatggct tacctgagac ttacctggga tacgagccgg cgcagccggc
      301 cgcagcgagt ggaggaaagg ggcggggatg ttacggaagc gaaggaggcc tggccagctc
      361 gccaagcgag tgcagagcgc ggttgcctgc gggcaagcac gccagaaccg caccgccaaa
      421 ccgcaggaaa gcgcttccac ctcggcctcg acccaggtcc acccgcaaat ccaccccagg
      481 ttcgaagcgg gcgccgaagg tgacctgcta aaaaaacagc ggtgaatggc cgaatcgtga
      541 ctccgtcacc gcgcaatcaa cggctacgac aacaacagaa aataggctga gagctcccaa
      601 tggctgacta caaagcgccc ctccgtgaca tggacttcgt gctcaatgaa gtctttgaag
      661 tttccaaaca atgggccgat ctgccggcgc tggccgaggt cgtcgacgcc gacactgcca
      721 aggccatcct cgaggaggcc ggcaaggtca gcgccggggt gctcgccccg ctgaaccgca
      781 gcggcgacga ggagggctgt tcctggcacg agggtgcggt gcagaccccg gccggttttc
      841 ccgcggccta ccaggcctac ggcgaaggcg gctgggtcgg cgtcggtggc tcgccggagt
      901 tcggcggcat gggcatgccc aaggtgatcg gcgcccaggt cgaggagatg atcaactcgg
      961 ccaacctgtc cttcgccctg tatccgatgc tgaccgccgg cgcctgtctg gcgctgctca
     1021 accacggcag cgaggagctg aaagcgcagt acctaccggc catgtacgaa ggccgctgga
     1081 ccggctccat gtgcctgacc gaatcgcacg ccggcaccga cctcggcatg atccgcacca
     1141 aggccgagcc acaggccgac ggcagctaca aggtcagcgg caccaagatc ttcatcaccg
     1201 gcggcgagca ggacctctcc gagaacatca tccacctggt actggccaag ctgccggacg
     1261 ctccggccgg tgccaagggc atctcgctgt tcctggtgcc caaggtgctg gtcaacgccg
     1321 acggctcgct gggcgcgcgc aatgcggtga gctgcggctc catcgagcac aagatgggca
     1381 tcaaggcctc cagcacctgc gtgatgaact tcgacggcgc caccggctac ctggtcggcg
     1441 agctgaacaa gggcctcaac gccatgttca ccatgatgaa ctacgagcgt ctgggcgtcg
     1501 gcatccaggg cctggcgctg ggcgagcgct cctaccagag cgccgtcgaa tacgcccgcg
     1561 agcgcatcca gagccgcgcg ccgaccggcg cggtggccag ggacaaggcg gccgacccga
     1621 tcatcgtgca tccggacgtg cgccgcatgc tgctgaccat gaaggcgctg aacgagggcg
     1681 gccgtgcctt ctccagcttc gttgccctgc agctcgacac cgccaagtac agcgaggacg
     1741 ccgtcgcccg caagcgcgcc gaagagctgg tcgccctgct taccccggtg gccaaggcct
     1801 tcctcactga catgggcctg gaaaccacta tccacggcca gcaggtgttc ggcggccacg
     1861 gctacatccg cgagtggggc caggaacaac tggtgcgcga ctgccgcatc acccagatct
     1921 acgaaggcac caacggcatc cagtcgctgg acctgatggg ccgcaaggtg gtcgccagcg
     1981 gcggcagcta ctaccggcat ctgtccgagg agatcaatgc gttcatcgcc ggcgccgatg
     2041 ccgcgctggg cgagttcact ggcccgctga aggccgccgt ggaaaacctc gacgagctga
     2101 cggcctgggt gctggacaag gcgcagaaca accccaacga gatcggcgcc gcctcggtcg
     2161 agtacctgca cgccttcggt tacaccgtgt acgcctacct gtgggcgcgc atggccgccg
     2221 tcgcgcgggc caagcagggc gagggcgact tctacgccag caagctcggc accgcgcgct
     2281 tctacttcgc ccgcatcctg ccgcgcatcc actcgctgag cgcctcgatc aaggccggca
     2341 gcgccagcct gtacctgctg gacgccgagc agttctgacc ggcgtgccgt ggcggtcgct
     2401 ccggccgcca cgacgctcga gcccgcaaag ggctgcaggc aactcagggg cttccttgct
     2461 cactgcgttt gagcgagagt gtactcctgt cggctctgac cggtggacgg tggcaaaggt
     2521 cgccgcggcg attccaaaaa caattgacgc tgaaacaagg ggtttacatg tccgatctgg
     2581 ttcgcatggg tgtcctgctg ggggcgctgg ctctgccggc agctgtcgtt gccgcggaga
     2641 attcaccgcc ggtggcggga agttcgcaat atgcacagga ggagcaggcg gcgagagcca
     2701 tgttggccaa ggctgtcgct tactatcgtc aggaaggcga gaaggcgctg gccacattca
     2761 gtcgccgtgg gccgttcact gtcgataacc tctacgtcta cgtggtcgac gcgcagggcg
     2821 tgctggtagc cagtggtggg ccgtcgatcc tgctggccgg tcaggatatc ggcgaggcgg
     2881 ttggcgccga ggcgcgtgcc aaactgatgt cccgggaagg tggcgagggg cacgtcgggg
     2941 agatcgaata ccccttccgc gactggaccc tcgacggcaa ggtcgtcaag aagcacacct
     3001 tctaccagtg ggtaggtgac cacgtcattg cggtcggcta ctacatgccg cgcgccgatt
     3061 cggccgatgc ccgcaagctg ctcgatgagg ccgcaaaagc ggtggccagc gagcccgaag
     3121 ccaccttaga cgccatcaac aagttcgaca agcgctacta ccagggcgat ctgtacgtcc
     3181 tcgtcgtcga cctgcagacc aagcgcttcg tcgcccacgg ctacaaccgg cgcctgctgg
     3241 cacgcgactt cgcatcgatc aaggggccgg atggcaagtt gatcggccag ccgatgctcg
     3301 acattgccct caacaagggc gagggcgagt tcgactatca gtggctgaac ccggtgacca
     3361 agcagaacga gccgaagcac gcctacatcc gtcgggtcgg caattacctg gtggcggtgg
     3421 gctactacca gccccagtga gggcctggta tcccagtcaa acaggacatg gagcgtcctt
     3481 gaaaaaagag ccactgccct atgcggggca gtggcgcttg catgacaggc tgatttactc
     3541 gaccgccttg agcatgtcct tgatgacctt cttggcgtcg tcggccgagc tgctcttcac
     3601 cgggcgctgt tcctggctgc ccgccgtggc ggtcgtaccg gcttcggcac cgaagcgccg
     3661 aggaagacgt tgaagaacga gcggttcatc gccttgcaca tgatgcggaa gaagatcgtg
     3721 cccgaggagc cgaccaggga gccggcgacg atcagcatcg agttgttcag cgagaagccg
     3781 atgccggcct ccgcccagcc cgagtagttg ttgagcatcg acagcaccct acaagaatcg
     3841 aaagtatcgt caggagcaca ggcagaaacc gcggggctcc ctggtttctg cgcttgcgca
     3901 ggtcttgaaa taacctcaca agccgttgtt gcgaaagagt ttcaaaaatg cgccgatcct
     3961 gtccagaaac cctcgctcca gaactagtct gaaagggccg ccgggatgcg ctcaaccgtc
     4021 ccgtacggtc gatgctcgtc ctttggtcgg tcacagcgat gttttcgtgg aggacacgag
     4081 cgatgaacag aaaagcttgg ctggcgttga tggttgcagc agtagcgagc cagagctacg
     4141 gaatgacgga tgagcgaggg ctggtttcaa tcgagctgaa ggccagccct cagaataccg
     4201 ccgagatcgg tcgggcgacc ctggtgaccg aggggagcag gacggcgatc gtcttcgccc
     4261 tcagtggcgc accgtcccat gtggcactgc ccctgcatta ctacacctac atctatcctg
     4321 gcagctgcaa tgccctcggc gcagagccag cctacgacat gaacgcagtc gtgcgggtgg
     4381 aaagcttcgg gcacctgtcg aagcggatcc cggtcgccct cgagcaattg cgtggtggaa
     4441 actacagcat tgtcgtgcgc agcagtcccg ccgatggccg cctcgacctt ttctgcgccg
     4501 agatcatcta gagcaggggt aaggagctgc ccctcggggc agctccgcct ggtcacatgt
     4561 tggggtaggt ggggccaccg gtgccttccg gcgccaccca ggtgatgttc tgcgccgggt
     4621 ccttgatgtc gcaggtcttg cagtgcacgc agttctgcgc gttgatctgg aacttcttct
     4681 cgccgctttc cagggtgacg atctcgtaca ccccggccgg gcagtagcgc tgcgccggct
     4741 cgtcgtacag cggcaggttc ttgcccagcg ggatcgaggc atcggccagc ttcaggtggc
     4801 agggctggtc ttcctcgtgg ttggtgttgg agaggaacac cgaggacagc ttgtcgaagc
     4861 tcagcttgcc gtccggcttg gggtagtcga tcttctgcgc ctcggcggcc ggcttcaggc
     4921 aggcgtagtc cggcttgttg tcgtgcaggg tcagcgggat cttgccgccg aacaggttct
     4981 ggtcgacgaa gttgaacgcg ccgccgagga tggcgccgaa cttgtggatc gccgggccga
     5041 agttgcggct gcagaacagt tcgtc
//
LOCUS       DATCUN010000244         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_236184, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000244 DATCUN010000000
VERSION     DATCUN010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236184"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VIO83_09320"
     CDS             complement(<1..407)
                     /locus_tag="VIO83_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HEY8331511.1"
                     /translation="MVYQWLKSRQPCLLCDLPADTPQPLCSDCERELPWLGPHCRVCA
                     LPLPADGLVCAHCLRRAPSFQRVEAPWRYAFPLDALITRFKHQSAWPFGRLLGALLAE
                     HLRHAYLEGLPRPDLLLPVPLARPRLRRRGFNQA"
     gene            522..1574
                     /gene="bioB"
                     /locus_tag="VIO83_09325"
     CDS             522..1574
                     /gene="bioB"
                     /locus_tag="VIO83_09325"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEY8331512.1"
                     /translation="MSANASLRHDWTLDEVRALYQQPFNDLLFQAQTAHRQHFNPNRV
                     QVSTLLSIKTGACPEDCKYCPQSGHYNTGLDKEKLMEVQKVLQAAAEAKAIGSTRFCM
                     GAAWKHPSAKDMPYVLEMVKGVKALGLETCMTLGRLDAEQTKALADAGLDYYNHNLDT
                     SPEFYGNIITTRTYAERLQTLGYVREAGMKICSGGILGMGESQDDRLGLLIQLANLPE
                     HPESVPINMLVKVQGTPLEKAEDVDPFDFIRTLAVARILMPESHVRLSAGREQMNDQM
                     QALAFFAGANSIFYGEKLLTTGNPDADRDMRLFERLGIQPEAREEHADEVHQAAIEQA
                     LIEQRNSALFHDAAAV"
BASE COUNT          288 a          551 c          520 g          218 t
ORIGIN      
        1 gcctggttga agccgcgccg gcgcaggcgg gggcgcgcca gcggcaccgg cagcagcagg
       61 tcggggcgcg gcaggccttc cagataggca tggcgcaggt gctcggccag cagcgcgccg
      121 agcaggcggc cgaacggcca ggcgctctgg tgcttgaagc gggtgatcag cgcatccagc
      181 gggaaggcat agcgccaggg ggcttcgacc cgctggaagc tgggcgcgcg gcgcaggcag
      241 tgggcgcaga ccaggccgtc cgccggcagc ggcagggcgc acacccggca gtgcgggccg
      301 agccagggca gctcgcgttc gcagtcgctg cacagcggtt gcggcgtatc ggcgggcaga
      361 tcgcagagca ggcagggctg cctgcttttt aaccattggt aaaccatgcg ttccctcgca
      421 gttgacagcg cgcgaggctg ccgtaaagat gcggccatcc ctgccgcccc gcgattctac
      481 ttgttcttcg ggcgcgctac cccctaacaa ggaaaccgcc catgagtgcc aacgcctccc
      541 tgcgccacga ctggaccctc gacgaggtcc gcgccctgta ccagcagccg ttcaacgatc
      601 tgctgttcca ggcgcagacc gcgcaccgcc agcacttcaa tcccaaccgt gtgcaggtct
      661 ccaccctgct gtcgatcaag accggcgcct gcccggaaga ctgcaagtac tgcccgcagt
      721 ctggccacta caacaccggc ctggacaaag agaagctgat ggaggtgcag aaggtcctgc
      781 aggccgccgc cgaggccaag gccatcggct cgacccgctt ctgcatgggc gcggcgtgga
      841 agcacccgtc ggccaaggac atgccctacg tgctggagat ggtcaagggc gtcaaggcgc
      901 tcggcctgga aacctgcatg accctcggcc gcctcgacgc ggagcagacc aaggcgctgg
      961 ccgatgccgg cctcgactac tacaaccaca acctcgacac ctcgccggag ttctacggca
     1021 acatcatcac cacccgcacc tacgccgagc gtctgcagac cctgggctac gtgcgcgagg
     1081 cggggatgaa gatctgctcc ggcggcatcc tcggcatggg cgagtcgcag gacgaccgtc
     1141 tgggcctgct gatccagctg gccaacctgc cggagcaccc ggaatcggta ccgatcaaca
     1201 tgctggtcaa ggtgcagggc accccgctgg agaaggccga ggacgtcgat ccgttcgact
     1261 tcatccgcac cctggcggtg gcgcgcatcc tcatgcccga gtcgcacgtg cgcctgtccg
     1321 ccggccgcga gcagatgaac gaccagatgc aggccctggc gttcttcgcc ggtgccaact
     1381 cgatcttcta cggcgagaag ctgctgacca ccggcaaccc ggacgccgac cgcgacatgc
     1441 gcctgttcga gcgcctgggc atccagccgg aagcccgcga ggaacacgcc gacgaggtgc
     1501 accaggccgc catcgagcag gcgctgatcg aacagcgcaa cagcgcgctg ttccacgacg
     1561 ctgcggcagt ctgagtc
//
LOCUS       DATCUN010000245         3864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_198722, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000245 DATCUN010000000
VERSION     DATCUN010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198722"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..112
                     /locus_tag="VIO83_09330"
     CDS             <1..112
                     /locus_tag="VIO83_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA base-flipping protein YbaZ"
                     /protein_id="HEY8331513.1"
                     /translation="AAGAEQRRRLAAEGVALTGNRVDIRRHGWHPEHQSS"
     gene            147..1691
                     /locus_tag="VIO83_09335"
     CDS             147..1691
                     /locus_tag="VIO83_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004881485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HEY8331514.1"
                     /translation="MPRRTWREAFATYASPSALALLLLGFAAGLPMVLVFSTLSVWLR
                     EAGVARETIGYASLIGLAYAFKWVWSPLLDQWRLPLLGRLGRRRSWLVLAQLLVAAGL
                     AGMALFDPQTHLAWLIAVAVLVAFASATQDIALDAYRLEIAEDTQQAALAACYTTGYR
                     VAMLLATAGALFVAEGFGSTGSNYLYPAWASTYLLFALLMLPGLLTTLWMGEPPVPQP
                     IQQAASRYGLTHQLISVVVLIVLLVSVPAMFTQLYHSDVQGILASRTTLFDLFRDDRA
                     FLRFTLYVILTLLCLSSFGRRGLAPVLTPISDFIQRYRWQALVLLGLIATYRMSDTVM
                     GVMANVFYVDLGFSKDQIASVSKLFGLVMTLLGAGVGGLLIVRFGILPILFLGGLASA
                     ATNLLFAMLSGMGPNLEMLVITISADNFSGGLATAAFVAYLSSLTNLQFSATQYALLS
                     SIMLLLPRLMGGYSGVMVESLGYADFFLVTALIGIPTLLLIGLQWGRERQQANGSALS
                     KPPQSS"
     gene            complement(1779..2258)
                     /locus_tag="VIO83_09340"
     CDS             complement(1779..2258)
                     /locus_tag="VIO83_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY8331515.1"
                     /translation="MPSFDVVSELDKHEVTNAVDNAIKELDRRYDLRGKGSFEFKEKT
                     VTLTAEADFMLEQMIEILKLSLVKRKIDVQCLEYKDSYASGKVVKQEVTFREGIDKEL
                     AKKIVALIKDHKLKVQAAIQGEQVRVTGKKRDDLQEAIALLRTQDLGMPLQYNNFRD"
     gene            2400..3368
                     /locus_tag="VIO83_09345"
     CDS             2400..3368
                     /locus_tag="VIO83_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8331516.1"
                     /translation="MPDPNLSIMVVDDARFSSALIGRALQQAGYRDIRYAGSASEALA
                     ELDRRPASVLLADWMMPEMDGLELTTQVRLLEEFGEHYTYIILLTGGDGDNLLTEAFD
                     RGVDDFVNKATVNEQLVPRVMAADRLCGTMQRLLQEKRLLAHNIANLEQRNVVDALTG
                     LGNLRYLRQRLGDALRQLESRGGALCYLLIGLEDLRSRHGEGFQRELLYNIARRLRNM
                     VRPLDVLVRVDDRQFAVVAMVNDLRECSPSSFRRLHDGLNLKALKTSEGFMQINAAVS
                     MVCVDASDAPLRIDDLLRQAAEQLDEARSSGRIASHRVKRTQASAP"
     gene            3365..>3864
                     /locus_tag="VIO83_09350"
     CDS             3365..>3864
                     /locus_tag="VIO83_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8331517.1"
                     /translation="MTWHVLGAGSLGGIWAARLTLAGLPVSLLLRDGNRLTQFQAAGG
                     LTFEQDGRCQTLPIAAETLAATTPIRRLLLASKAYDAAAVARALRPRLAADAEVVLLQ
                     NGLGSQDEVAALLPQARCLFASSTEGAFRRADFHVVFAGAGHTWLGSVPATPAPDWLA
                     ELAQAQ"
BASE COUNT          571 a         1400 c         1206 g          687 t
ORIGIN      
        1 ggcggccggc gccgagcagc gccgccgctt ggccgccgag ggcgtggcgc tgacgggcaa
       61 ccgggtggac atccgtcgcc atggctggca tcccgagcac cagagcagtt agagtgcata
      121 ctttgtcccg gaccctgcag cactccatgc cccgtcgaac ctggcgcgaa gcgttcgcca
      181 cctatgccag cccctccgca ctcgccctcc tgctgctcgg cttcgccgcc ggcctgccga
      241 tggtgctggt gttctccacc ctgtcggtgt ggctgcgcga agccggcgtg gcgcgcgaga
      301 ccattggtta cgccagcctg atcggcctgg cctacgcctt caagtgggtg tggtcgccgc
      361 tgctcgacca gtggcgactg ccgctgctcg gccgcctcgg ccggcgccgc tcctggctgg
      421 tcctggcgca attgctggtg gccgcaggtc tggccggcat ggcgctgttc gacccgcaga
      481 cccacctcgc ctggctgatc gccgtggcgg tgctggtggc cttcgcctcg gccacccagg
      541 acatcgccct cgacgcctac cgcctggaga tcgccgagga cacccagcag gccgccctgg
      601 ccgcctgcta taccaccggc taccgggtcg ccatgctgct ggccaccgcc ggcgcgctgt
      661 tcgtcgccga gggcttcggc tcgaccggca gcaactacct gtacccggcc tgggccagta
      721 cctacctgct gttcgccctg ctgatgctgc ccggcctgct gaccacgctg tggatgggcg
      781 aaccgccggt accgcaaccg atccagcagg ccgcctcccg ctacggcctg actcaccaac
      841 tgatttcggt tgtggtgctg atcgtcctgc tggtgtcggt gccggcgatg ttcacccagc
      901 tctaccacag cgacgtgcag ggcatactgg ccagccgcac gaccctgttc gatctgttcc
      961 gcgacgaccg cgccttcctg cgcttcaccc tctacgtcat cctcaccctg ctgtgcctgt
     1021 cgtccttcgg ccgccgcggc ctggcaccgg tgctgacgcc gatcagcgac ttcatccagc
     1081 gctatcgctg gcaggcgctg gtgctgctcg gcttgatcgc cacctaccgc atgtcggaca
     1141 cggtgatggg cgtgatggcc aacgtgttct acgtcgacct cggcttcagc aaggatcaga
     1201 tcgccagcgt cagcaagctg ttcggcctgg tcatgaccct gctcggcgcc ggcgtgggcg
     1261 gcctgctgat cgtgcgcttc ggcatcctgc cgatcctgtt cctcggcggc ctggcctcgg
     1321 cggcgaccaa tctgctgttc gccatgctct ccggcatggg gccgaacctc gagatgctgg
     1381 tgatcaccat ctcggcggac aacttcagcg gcggcctggc caccgccgcc ttcgtcgcct
     1441 atctgtcgag cctgaccaac ctgcagtttt ccgccaccca gtacgccctg ctcagctcga
     1501 tcatgctgct gctgccgcgc ctgatgggcg gctactcggg ggtgatggtg gagagcctcg
     1561 gctatgctga cttcttcctg gtcaccgcgc tgatcggcat tccgaccctg ctgctgatcg
     1621 gcctgcagtg gggccgcgag cgccagcagg ccaacggcag tgcgctgagc aaaccgccac
     1681 agtcgtcctg aacgggcagg gtgcagaacg gcaaaggcca gcctcgagag gctggccttt
     1741 gactatccga ccgaggagcc cgcgagcgcg gagaaccatc agtcgcggaa gttgttgtac
     1801 tgcagcggca tgcccagatc ctgggtacgc agcagggcaa tggcctcctg caggtcgtca
     1861 cgcttcttgc cggtgacgcg cacctgctca ccctggatcg cggcctggac cttgagcttg
     1921 tgatccttga tcagggcgac gatcttcttc gccagctcct tgtcgatgcc ttcgcgaaac
     1981 gtcacctcct gcttgaccac cttgccggac gcataggagt ccttgtactc caggcactgg
     2041 acatcgatct tgcgcttgac cagtgacagc ttgaggatct cgatcatctg ctcgagcatg
     2101 aagtcggcct cggccgtcag ggtgacggtc ttctccttga attcgaaact gcccttgccg
     2161 cgcaggtcat agcggcgatc cagttccttg atggcgttgt cgacagcgtt ggtcacttcg
     2221 tgtttgtcca gttcggacac cacgtcgaac gagggcatgg ggactctcca gatggacggc
     2281 gcgacccggt tcacggatgc atcgcgcctg gcttgcggta aaaatgagcc ctcattataa
     2341 ctgagcgtct caatggtgcc gcacccgcca cctccatccc cttcgccgag cccctgccca
     2401 tgcccgatcc caacctcagc atcatggtgg tggacgacgc ccggttttcc agcgcgctga
     2461 tcggccgcgc cctgcagcag gccggctacc gtgacatccg ctacgccggt agcgccagcg
     2521 aggcgctcgc cgaactcgac cggcgcccgg ccagcgtgct gctggccgac tggatgatgc
     2581 cggaaatgga cgggctggag ctgaccaccc aggtccgtct gctcgaagag ttcggcgagc
     2641 actacaccta catcatcctg ctcaccggcg gtgacggcga caatctgctg accgaggcct
     2701 tcgaccgcgg cgtcgacgac ttcgtcaaca aggccaccgt caacgagcaa ctggtgccac
     2761 gggtaatggc cgccgaccgc ctgtgcggca ccatgcaacg tctgctgcag gaaaaacgcc
     2821 tgctggcgca caatatcgcc aacctcgagc agcgcaacgt ggtcgatgcg ctgaccggcc
     2881 tgggcaacct gcgctacctg cgccagcgcc tcggcgacgc cctgcgccag ctcgagtcgc
     2941 gcggcggcgc gctgtgctac ctgctgatcg gcctggagga tctgcgcagc cggcatggcg
     3001 agggcttcca gcgcgagttg ctgtacaaca tcgcgcgccg cctgcgcaac atggtgcgcc
     3061 cgctggatgt tctggtgcgc gtggacgacc ggcagttcgc cgtggtcgcc atggtcaacg
     3121 acctccgcga gtgcagcccc agcagtttcc gccgcctgca cgacggcctc aacctcaagg
     3181 cgctgaagac cagcgagggc tttatgcaga tcaacgccgc cgtgtcgatg gtctgcgtcg
     3241 acgccagcga tgccccgctg cgcatcgatg acttgctccg ccaggccgcc gagcagctgg
     3301 acgaggcccg tagcagcggg cgcatcgcca gccaccgagt caaacgcacc caagcctccg
     3361 cgccatgacc tggcacgtgc tcggtgccgg cagtctcggc gggatctggg ccgcgcgcct
     3421 gaccctggcc ggcctgccgg tatccctgct gctgcgcgac ggcaaccgtc tcacccagtt
     3481 ccaagccgcc ggcggcctga ccttcgagca ggacggccgc tgccagaccc tgccgatagc
     3541 tgccgaaacc ctcgccgcaa ccacgccgat ccgccgcctg ctgctggcga gcaaggccta
     3601 cgatgccgcc gcggtcgccc gcgccctgcg cccgcgcctg gccgcggacg ccgaggtcgt
     3661 cctgctgcag aacggcctgg gcagccagga cgaagtcgcc gccctgctgc cgcaggcacg
     3721 ctgcctgttc gcctccagca ccgaaggcgc cttccgtcgc gccgacttcc acgtggtgtt
     3781 cgccggcgcc gggcacacct ggctcggcag cgtgccggca acgccggcac cggactggct
     3841 ggccgaactg gcgcaggcgc agat
//
LOCUS       DATCUN010000246         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_123865, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000246 DATCUN010000000
VERSION     DATCUN010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123865"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1702)
                     /locus_tag="VIO83_09355"
     CDS             complement(23..1702)
                     /locus_tag="VIO83_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8331518.1"
                     /translation="MNLPSYTCGAQEQPLLAMTIGAAFERTVERFAEREALVVRDQGL
                     RFTWAELAAAVDRCARAFIALGLQPGERLGIWSPNRAEWTIAQFASAKAGVVLVNVNP
                     AYRLTELEYALKQSGCRWLICADTFKTSDYHGMLAELLPELAASAIGALQSHMLPELR
                     GVISLGAQPADGMLGWQALQAMAEQVGPEQLRECGEQLQFDEPINIQYTSGTTGFPKG
                     ATLSHYNILNNGYLVGESLGLTEHDRLVIPVPLYHCFGMVMGNLGCLTHGSTMIYPSA
                     AFEPLAALQAVAEERATALYGVPTMFIAMLDYPERGGFDLSSLRTGIMAGATCPIEVM
                     KRVIAEMHMKEVQIAYGMTETSPVSTQTGPSDDLERRVTSVGRTQPHLESKIVDEQGR
                     IVPRGQIGELCTRGYSVMLGYWNNPEATREALDGARWMHTGDLATMDDEGYIKIVGRN
                     KDMIIRGGENVYPREIEEFLFTHPAVADVQVIGVPDSKFGEEIVAWVKLHPGQAVEPE
                     ALRGYCKGRIAHFKTPRHIKFVDDFPMTISGKVQKFRMREISVLELGITQH"
     gene            1988..>2602
                     /locus_tag="VIO83_09360"
     CDS             1988..>2602
                     /locus_tag="VIO83_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8331519.1"
                     /translation="MTYSSLNFALGETIDMLRDQVRAFVTSELAPRAAAIDHDNLFPA
                     DMWKKFGEMGLLGVTVAEEYGGAGLGYLAHVVAMEEISRASASVALSYGAHSNLCVNQ
                     INRNGNPAQKAKYLPKLISGEHVGALAMSEPNAGSDVVSMKLRADKKGDRFVLNGSKT
                     WITNGPDANTYVIYAKTDLDKGAHGITAFIVERDWKGFSRGSKFD"
BASE COUNT          436 a          862 c          861 g          443 t
ORIGIN      
        1 acgatccccc tctcctgccg cctcagtgct gggtgatccc cagctccagc acgctgatct
       61 cgcgcatgcg gaacttctgc accttgccgc tgatggtcat cgggaagtcg tcgacgaact
      121 tgatgtgccg cggcgtcttg aagtgggcga tgcggccctt gcagtagccg cgcagcgcct
      181 cgggctcgac cgcctggccg ggatgcagct tgacccaggc gacgatctcc tcgccgaact
      241 tgctgtccgg cacgccgatg acctgcacgt cggccaccgc cggatgggtg aagaggaact
      301 cctcgatctc gcgcggatac acgttctcgc caccgcggat gatcatgtcc ttgttgcgcc
      361 cgacgatctt gatgtagccc tcgtcgtcca tggtcgctag gtcgccggtg tgcatccagc
      421 gcgcgccgtc gagggcctcg cgggtcgcct cggggttgtt ccagtagccg agcatcacgc
      481 tgtagccgcg ggtgcacagc tcgccgatct gcccgcgcgg cacgatgcgc ccctgctcgt
      541 cgacgatctt gctctccagg tgcggctggg tgcggccgac gctggtgacg cggcgctcga
      601 ggtcgtcgct cgggccggtc tgggtcgaca ccgggctggt ctcggtcatg ccgtaggcga
      661 tctgcacctc cttcatgtgc atctcggcga tcacccgctt catcacctcg atcgggcagg
      721 tggcgccggc catgatcccg gtgcgcaggc tggacaggtc gaagccgccg cgctcggggt
      781 agtcgagcat ggcgatgaac atggtcggca cgccgtacag cgcggtggcg cgttcctcgg
      841 cgaccgcctg cagggcggcc agcggctcga aggcggcgct cgggtagatc atggtgctgc
      901 cgtgggtcag gcagccgagg ttgcccatca ccatgccgaa gcagtggtac agcggcaccg
      961 ggatgaccag gcggtcgtgc tcggtcaggc cgaggctctc gccgaccagg tagccgttgt
     1021 tgaggatgtt gtagtggctg agggtggcgc ccttggggaa gccggtggtg ccggaggtgt
     1081 actggatgtt gatcggctcg tcgaactgca gctgctcgcc gcattcgcgc agctgctccg
     1141 ggccgacctg ctcggccatg gcctgcagcg cctgccagcc gagcatgccg tcggccggct
     1201 gcgcgccgag gctgatcacc ccgcgcagct ccggcagcat gtggctctgc agcgcgccga
     1261 tggcactggc ggccagctcc ggcagcagct cggcgagcat gccatggtag tcggaggtct
     1321 tgaaggtgtc ggcgcagatc agccagcgac agccggactg cttgagcgcg tattcgagtt
     1381 cggtgaggcg gtaggccgga ttgacgttga ccagcaccac gccggccttg gcgctggcga
     1441 actgggcgat ggtccactcg gcgcggttcg gcgaccagat gccgaggcgc tcgcccggct
     1501 gcaggccgag ggcgatgaag gcgcgggcac agcggtcgac cgccgcggcc agctcggccc
     1561 aggtgaaacg caggccctgg tcgcgcacca ccagcgcctc gcgctcggca aaacgctcaa
     1621 ccgtgcgctc gaaggccgca ccgatggtca tcgccaacaa cggttgctcc tgcgctccgc
     1681 aggtatagct cggaagattc atagaatccc tcagtcttat gtttgttatg aggtttgcca
     1741 cttctgagtc ttgctgttcc ggcgccgact tcgcagttga cagtcgccgc ggggtttgtt
     1801 tacgttaacg taaaggtgac tacctgacaa gccaccagaa agcgtcgccg gacacacttc
     1861 ggcactacgc tgaaagcaga gttcaccatc tctgcggcac gtcgggatta acatgccgga
     1921 gcccccggca acggccaccg aacgactccg caaaccgcac aactacaaga cagacaaagg
     1981 tggttccatg acgtactcca gcctcaactt cgccctcggc gaaaccatcg acatgctgcg
     2041 cgaccaggtg cgtgccttcg tgaccagcga gctggcgccg cgcgccgccg ccatcgacca
     2101 cgacaacctg ttccccgccg acatgtggaa gaagttcggc gagatgggcc tgctgggggt
     2161 gaccgtggcc gaggagtacg gcggcgcggg tctcggctac ctggcccacg tggtggccat
     2221 ggaagaaatc agccgcgcct cggcctcggt cgcgctgtcc tacggcgccc actccaacct
     2281 gtgcgtcaac cagatcaacc gcaacggcaa cccggcgcag aaggccaaat acctgcccaa
     2341 gctgatcagt ggcgagcacg tcggcgcgct ggcgatgagc gagccgaatg ccggctccga
     2401 cgtggtgtcg atgaagctgc gcgcggacaa gaaaggcgac cgcttcgtcc tcaacggcag
     2461 caagacctgg atcaccaacg gtccggacgc caacacctac gtgatctacg ccaagaccga
     2521 cctcgacaag ggcgcgcacg gcatcaccgc gttcatcgtc gagcgcgact ggaagggctt
     2581 cagccgcggc agcaagttcg ac
//
LOCUS       DATCUN010000247         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_180837, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000247 DATCUN010000000
VERSION     DATCUN010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180837"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VIO83_09365"
     CDS             <1..184
                     /locus_tag="VIO83_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331520.1"
                     /translation="VLELLEEINAAGSTIVMVTHDPELAARAQRNVHIVDGQATDLVR
                     EAGLMASAAPDLSAQA"
     gene            417..1721
                     /locus_tag="VIO83_09370"
     CDS             417..1721
                     /locus_tag="VIO83_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8331521.1"
                     /translation="MFTYYCNLALRSFKRNKVLTALMVLAIALGIGASMTTLTVFHVL
                     SGDPIPGKSERLFRVQLDPEGIDGYTPGEEPQRDLNRYDGETLLLQKQGKRQALMSGG
                     SVTVEPAGGALRPFMLDARYTSADFFAMFEVPFLFGHGWSANEDAGQARVAVIARTLN
                     DKLFNGADSTGRTLRIDGHDFQIVGVINDWKPKPLFYDPDIGTYDTEEQVFEPFQTAI
                     GLEQERNGSMNCYGATATVDGTAREAPCAWAQYWVELDSASRAGDYKAYLDRYSQQQK
                     EAGRYQRPPNTRLRNVMDWLEYNDAVPGDVRLQLWLAMGFLLVCLVNTVGLLLAKFLR
                     RSGEIGVRRALGASRAEIFKQCLVEAGSIGLAGGVLGLGLAELGLWAVRHRPTGYAAL
                     AQLDGSMLLMTFVLAVCASLLAGLLPAWRAMQITPAVQLKTQ"
BASE COUNT          329 a          683 c          625 g          303 t
ORIGIN      
        1 cgtgctggaa ctgctggagg agatcaacgc cgccggcagc accatcgtca tggtcaccca
       61 cgacccggaa ctggccgccc gcgcccagcg caacgtgcac atcgtcgacg gccaggccac
      121 cgacctggtg cgcgaggcgg gattgatggc ctcggctgca ccagacctct ccgcacaggc
      181 gtaattggat gggctccccc cgcctcgcac cgcgccaccc aggcccgtag ggcgggtctc
      241 gacccgcctt tcggtctcga ctcgccttcc ggtctcgact cgcctttcac cctcaacccg
      301 ccatccggga atctcgcccc gcctcgtgca ccgccccacg ccagccaccc ggcgggtcaa
      361 gacccgcccc acacaagccc ttggcgggtc aagacccgcc ctaccgggaa acccccatgt
      421 tcacctacta ctgcaacctt gcgttacgca gcttcaagcg caacaaggtg ctgaccgcgc
      481 tgatggtgct ggccatcgcg ctgggcatcg gtgcctcgat gaccacgctg acggtgttcc
      541 acgtgctgtc gggcgatccg atcccgggca agagcgagcg gctgttccgc gtgcagctgg
      601 atccggaagg gatcgacggc tatacacccg gcgaggagcc gcagcgggac cttaaccgct
      661 acgacggcga gaccctgctg ctgcagaagc agggcaagcg ccaggcgctg atgagcggcg
      721 gcagcgtcac ggtcgaaccg gccggcggcg cactgcggcc tttcatgctg gatgcgcgct
      781 acacctcggc cgatttcttc gcgatgttcg aggtgccctt cctgttcggc cacggttggt
      841 cggccaacga agatgccggc caggcacgcg tggcggtgat cgccaggacg ctcaacgaca
      901 agctgttcaa cggcgccgac agcaccggca ggaccctgcg catcgatggc catgatttcc
      961 agatcgtggg cgtgatcaac gactggaagc ccaagccgct gttctatgac cccgacatcg
     1021 gcacctacga caccgaggag caggtgttcg agccgttcca gaccgccatc ggcctggaac
     1081 aggaacgcaa cggcagcatg aactgctacg gcgccaccgc caccgtcgac ggcactgccc
     1141 gtgaagcgcc atgcgcctgg gcccagtact gggtagagct ggacagtgcg tccagggccg
     1201 gcgactacaa ggcctacctg gatcgctatt cgcagcagca gaaagaggcc ggtcgctacc
     1261 agcgcccgcc caacacgcgc ctgcgcaacg tcatggactg gctggaatac aacgacgcgg
     1321 tgcccggcga cgtgcgcctg cagctgtggc tggcgatggg ctttttgctg gtgtgcctgg
     1381 tcaacacggt cggcctgctg ctggccaagt tcctgcgccg cagcggcgag atcggcgtgc
     1441 gccgcgcgct gggcgccagc cgcgccgaaa tcttcaagca gtgcctggtc gaagccggca
     1501 gcatcggcct ggccggcggc gtgctgggcc tgggactggc tgagctcggc ctgtgggccg
     1561 tgcgccatcg ccccaccggt tacgcggcgc tggcgcagct ggatggctcg atgctgttga
     1621 tgaccttcgt gctggcggtg tgcgccagcc tgctggccgg cctgctgccg gcgtggcgcg
     1681 ccatgcagat cactccggcg gtgcaactga aaacgcagtg acattcccct cccctttcgc
     1741 gcgcagcgcg aagggggagg tcgggagggg gtgcgtttgg ctgtgcaggc cagggcatgc
     1801 cagaagcaac ccctccccag ccctcccctt ggccgatggc caaagggagg gagcaaaagc
     1861 tggcttgctc catccgaccc accacttcgg tgccgaaccc gtaccccaac gcctctcaag
     1921 gacgaggggc tcaaagcttc
//
LOCUS       DATCUN010000248        34138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_67225, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000248 DATCUN010000000
VERSION     DATCUN010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 34138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34138
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67225"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..141
                     /locus_tag="VIO83_09375"
     tRNA            66..141
                     /locus_tag="VIO83_09375"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Lys,seq:ttt)
     gene            complement(202..1305)
                     /locus_tag="VIO83_09380"
     CDS             complement(202..1305)
                     /locus_tag="VIO83_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY8331522.1"
                     /translation="MVRARGQNRAEGSADGRMELQQGSMMGRKPTKPGAIPRFRKRKQ
                     KSGVVHFYYDHGGKPRKETPLGSDYGLAIKKWAELEHAGAIPAGAALTFRQAAERYQA
                     EVIPKKAPGTQAVNMRELSMLKRYFDDPPCPLESIKPRHVHNYLLSRKDAPVSANREK
                     SLLSHLWNWCRQKGYTDLPNPCLGINRNRESGRDVYVEDDVLQQVYDAADQVLRDAMD
                     LAYLTGQRPADVRELDRRNIKDGALVLRQGKTKTPMRISITGELKLLIDRLVNRDRPD
                     DAPISTRLLLGEDWLPIGKDALRYRFDRARERAGIPKDQFQFRDLRAKAGTDKADSAG
                     DIRKAQQQLGHQSVTTTEIYVRKRRGALTSPTK"
     gene            complement(1230..1442)
                     /locus_tag="VIO83_09385"
     CDS             complement(1230..1442)
                     /locus_tag="VIO83_09385"
                     /inference="COORDINATES: protein motif:HMM:NF025352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HEY8331523.1"
                     /translation="MADSPYLSRDDIRDLCRTPQRKRQAAFLRTNGIRHYLDAYGWPV
                     VLWSALEGKTEPKAAPTAEWNSNKAA"
     gene            complement(1458..2216)
                     /locus_tag="VIO83_09390"
     CDS             complement(1458..2216)
                     /locus_tag="VIO83_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEY8331524.1"
                     /translation="MAELLPILVACEYSDTVASAFRARGFDAYSCDLRPTEGDPRWHI
                     QDDVRNHLQAGRWLAMIAHPYCTYNTLAGIRWMYHPDDTALPQADRRRHPKFPNRMRD
                     FLEGALLFQDLMAAPIEFIGNENSKPHGLAMSVLGRPTQTVQPYDFGSPFTKGATLWL
                     KNLPALVPTHTKAEVIAVHGKIEARCHLMPPGPDREKERSRFDPAIAAAMAQQWGGHI
                     LEAKAARRTQVVMPTDLFAAHQVTEQLRAALATN"
     gene            complement(2209..2361)
                     /locus_tag="VIO83_09395"
     CDS             complement(2209..2361)
                     /locus_tag="VIO83_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331525.1"
                     /translation="MSDALTLWLGKYLVLALMLVLGFLTVWLVVAFCRAVRWCWRRAA
                     RLVAHG"
     gene            complement(2354..2626)
                     /locus_tag="VIO83_09400"
     CDS             complement(2354..2626)
                     /locus_tag="VIO83_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331526.1"
                     /translation="MSQIIVFPRGQLSTADRKRMADAGILAVEADDPKSVVTVIPGVP
                     LATSDDLAMSALAAIAGCNINGVGMDFVKELHARLQVREAKEPTNV"
     gene            complement(2623..4029)
                     /locus_tag="VIO83_09405"
     CDS             complement(2623..4029)
                     /locus_tag="VIO83_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331527.1"
                     /translation="MNTERTEKFVNAIACDLEEGGFRQVSGTMLRRTLATAVSAALPL
                     LQPLPGTQSSRWAADGQSDPHGDRYACERAALALGHLTDDELANAVYLHGNEQPSMAD
                     LVAGKALSGIVYLTAAKERIRWLSRALSGATAPQPAELAEQRGMELPSLPASSAHDPR
                     TGEPLFSESKMRTFAKAALAATGKQQVGEESAGLPEMCRSSTNAQGMQVGEVQGDART
                     QFEAWAHSESLSLHKSARDGEYNGARTISAWLAWQAALAARQPGTQMPALTFSAFRNA
                     NVSRCEKWHPEGIGSWSPSDWLTAAMGELGELASEVKMHNRVRDGLAGNKEQITPEER
                     TRRMANEAADVVTYLDLFCAERGIDLGAALVRKFNEVSERVGFPDRLDPAAAQGIDLG
                     RALDIMWNWQEQLGGHVFDARDVARTLTSSDMQAIGVDARACIDATNRNQAIVLAKRE
                     IVRALSSGQRDAAPGVGA"
     gene            complement(4026..4283)
                     /locus_tag="VIO83_09410"
     CDS             complement(4026..4283)
                     /locus_tag="VIO83_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331528.1"
                     /translation="MSRPITRRAPKRNGGFSWGRFPMGDTGITAYRLSRRDHAGAIHI
                     VNLNFYPQDQRRAVALALRAACHRLRDRVDEIDLATMGVTA"
     gene            complement(4280..4891)
                     /locus_tag="VIO83_09415"
     CDS             complement(4280..4891)
                     /locus_tag="VIO83_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331529.1"
                     /translation="MSKERPILFNGAMVRAILAGQKTQTRRVLKMPHGYWETSADGLV
                     PIPAGCPFGQPGDRLWVRESFSPIYSTTDGRLIEVDYRATYQHGHRMGDHLGMPKEWK
                     PSVHMPREACRLVLEITSVRVERLQALPSGDWESEGISFCIEDPDTTAGRAFSEAEHY
                     WIAGVSMRGTPEDHAMRAHFAEQGLDWDSNPWVWVIDFKQVTP"
     gene            complement(4888..5112)
                     /locus_tag="VIO83_09420"
     CDS             complement(4888..5112)
                     /locus_tag="VIO83_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331530.1"
                     /translation="MTSIHAQTVFDAATARQKAAEAEQLAADVAEFERKGGRIQRLGN
                     TPIDRSGISRRQVVEGGAERRKAAKKGAGA"
     gene            complement(5109..5315)
                     /locus_tag="VIO83_09425"
     CDS             complement(5109..5315)
                     /locus_tag="VIO83_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331531.1"
                     /translation="MSNANYSACQLTKRELIAAMAMQGMGNWVPNADHGPFNWDSPQA
                     VGELKARWAIAQADALLAELERTA"
     gene            complement(5312..5548)
                     /locus_tag="VIO83_09430"
     CDS             complement(5312..5548)
                     /locus_tag="VIO83_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331532.1"
                     /translation="MSARKACTGCCRRLPIEAFPSAGRRGKEPTCAMCTNDARRLRTP
                     LPALKPDPVQVQLNNTASLWFYPAQPTNLLRYAP"
     gene            complement(5545..5766)
                     /locus_tag="VIO83_09435"
     CDS             complement(5545..5766)
                     /locus_tag="VIO83_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331533.1"
                     /translation="MDVRIYPYHRKSCVRAGNARAALFAHVVDGKRYTTKQVATQLGL
                     SLNGAAKRIKRGPYPLTWSTLQQSRLVKS"
     gene            complement(5766..6380)
                     /locus_tag="VIO83_09440"
     CDS             complement(5766..6380)
                     /locus_tag="VIO83_09440"
                     /inference="COORDINATES: protein motif:HMM:NF021128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEY8331534.1"
                     /translation="MRILTCEQRSPEWYAARRGVPTASEFGSIITPKKREYAAAADTY
                     INQLIDEAVRPDAGQAFTGNRHTERGVLLEDDARELYAFEHDVVPQQVGFILNDAGTL
                     GCSPDSLIGKPGGLEIKCPDGPTHVKWLRAGGVPDEHKPQVHGCLIITEREWWDFMSY
                     CPGYAPLVVRVTRDGFTDKLQEYLDRFLGEYAAARRLFDLPVAA"
     gene            complement(6384..7136)
                     /locus_tag="VIO83_09445"
     CDS             complement(6384..7136)
                     /locus_tag="VIO83_09445"
                     /inference="COORDINATES: protein motif:HMM:NF016302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HEY8331535.1"
                     /translation="MNEITTIAGSSQAGTGLQAALQQALMLPEQGVERLERMWEMHKD
                     MQDREAAREYATAMQRAQSSMPNIAKRGKNKQTNSQYALLEDINQAITPVYTREGFSL
                     SFGTVTSDLQGHVGIVCDVLHTGGHSRTYHYDAPIDDVGIKGEKNKTSTHGRGSAISY
                     GRRYLILMIFNLTIGGEDDDGNGAGENEAQRIAREIAQDWVRLAEGLKTLEEYTARKG
                     EVLQAYGGKASNLPPEVRAAFNRAAEATKPKD"
     gene            complement(7201..7938)
                     /locus_tag="VIO83_09450"
     CDS             complement(7201..7938)
                     /locus_tag="VIO83_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331536.1"
                     /translation="MTAHAPELKKLLTAAAHFTPKNSFIAGGALTSIFTGQPINDVDL
                     YFKTKEAFMDAVRAAYDEGLWCVAATDRAVTFAYGEDIIQLMLFDFFDTAEAIFSAFD
                     FTACMAAYDVDRETFVFHPDFFKHNAQRHLSFHSGTRYPFGSLLRTIKYRERGYKLGR
                     GDLLRIALCCHGVSLESWDDLAAAIGGQYGERVLLETDQPFSVAAAIELLSSAEFTVP
                     AAPEEMPGDADELLAKVGLLPENAIPA"
     gene            complement(8162..8560)
                     /locus_tag="VIO83_09455"
     CDS             complement(8162..8560)
                     /locus_tag="VIO83_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331537.1"
                     /translation="MSSKHTPGPWAVHDHPTDPYQYGHHVTTEDGLTICSVTYQLPVS
                     TPHGVEEATRVANARLIAAAPELLAELQRAQEFIDSIRQELHQRRVADWYPEGAHNAA
                     DQMSADMRLLGNTCADFDTSAVIAKATGAA"
     gene            complement(8550..8708)
                     /locus_tag="VIO83_09460"
     CDS             complement(8550..8708)
                     /locus_tag="VIO83_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331538.1"
                     /translation="MTPCSELARLFAQPFPLITPPVSLADAALAEAHMTHHEAINGLC
                     AEDSNDEQ"
     gene            complement(8705..8893)
                     /locus_tag="VIO83_09465"
     CDS             complement(8705..8893)
                     /locus_tag="VIO83_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331539.1"
                     /translation="MNLIEKTNHLGIAIEYVEFESVPVQQVQTVSREIQQHVAAINAR
                     QAVSQAKAAAAYAEGARS"
     gene            complement(9086..9325)
                     /locus_tag="VIO83_09470"
     CDS             complement(9086..9325)
                     /locus_tag="VIO83_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331540.1"
                     /translation="MSAPTITTTEPVHISEIRAGDTVSHNGCLRTVGRSDIKRGGFMG
                     TTLFGDSYRLGHTLVERVTFNRPTAIAADSEGGAA"
     gene            complement(9322..9672)
                     /locus_tag="VIO83_09475"
     CDS             complement(9322..9672)
                     /locus_tag="VIO83_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331541.1"
                     /translation="MQRATLNDNFRNAQRSWDSLEDPRFESELAGQQAAELAEAMRSD
                     PAACREAEQWVAGTFDGDHYTDVTLALHQLHHTDPSDLIDSPLLAQLYALAAVQAAAL
                     DEQLLEAALQQVAA"
     gene            complement(9803..10084)
                     /locus_tag="VIO83_09480"
     CDS             complement(9803..10084)
                     /locus_tag="VIO83_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331542.1"
                     /translation="MKLIFSATALLLLSACSAQEDTQALDVARDAMAAAQSASSAAEA
                     LGDEVEALQSRVDSLEQENRQQQSEIEDLEGRLGSVETKAIDLDGRIPY"
     gene            complement(10097..10768)
                     /locus_tag="VIO83_09485"
     CDS             complement(10097..10768)
                     /locus_tag="VIO83_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEY8331543.1"
                     /translation="MKTDTPAVALRRRRLKQWIDDRHDGSQASFVLATGVNQGELSSL
                     LRDKSFGEKRAAALEKAAGMPDGYLSSDAPQEVLSAETAPGYVRFDLFEGGAGMGQGL
                     VNQDYPEVVKTIAVAEWEVRRKLGFLPKPGRIQLITGRGPSMRPKLEDGDIVWIDTSC
                     DYFDGDDFYLINIGGETQIKMLQKRGDGLYVVSVNPEFPAYRSEASDVAILGKALIHA
                     GLRRF"
     gene            10846..11073
                     /locus_tag="VIO83_09490"
     CDS             10846..11073
                     /locus_tag="VIO83_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331544.1"
                     /translation="MDTLRTYLSTLTPAEQAAYAVRAGTSIGYLRKALSKGQRFDGGL
                     VRLLHVESDGQVSLTELRPDIWPENATDAVA"
     gene            11345..11824
                     /locus_tag="VIO83_09495"
     CDS             11345..11824
                     /locus_tag="VIO83_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY8331545.1"
                     /translation="MTQITQQKVREFLVYEPDTGLFRYRSGTRAGQVSGHKHHGYIKI
                     SIPGHGGIYAHRLAWLYMTGEMPPMFIDHIDCVRDNNEWANLRLADRSQNSANAKIKK
                     TNTSGFKGVRLFGGIFRAKITVQRRIIHLGDYETLEEAVAAYKGASRVGFGEYARTR"
     gene            11824..12168
                     /locus_tag="VIO83_09500"
     CDS             11824..12168
                     /locus_tag="VIO83_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331546.1"
                     /translation="MQQLNISFEVGLDRRYRDMRECFASCVYSKGLGRVAAAIDVAPS
                     NLSAMLAGDRNLDSTLVEKYMAEFGDTTPAMYWAAKHLQCATTRQQAALAQLPSLVDQ
                     LNRLMAEAGKTP"
     gene            12247..13176
                     /locus_tag="VIO83_09505"
     CDS             12247..13176
                     /locus_tag="VIO83_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331547.1"
                     /translation="MRDYAKIVPTFWTGDTGKAIRKRGAEGVVVALYLMSSPTSNMLG
                     LYYQPVLYMAHETGLGIGGASEGLRYCIEAGFCQYDEVTEFVWVPEMAAFQIGEGLKA
                     NDNRCVGIQKDYNGLPDNPFLGDFFDRYQADFHLRNRRGGEGVAKPLSSQEQEQEQEQ
                     EQERTSSLRSDSSPQLALTGTTPAPADHKPSRAERIRQIAGDAQAAFNATLAKPVGLL
                     AKCTVLNGPRIKAVEKALPTVRQICLQLFGSERVTPQFWQLYFETAADDDFYAGRVKG
                     GQGHENYVPDFALLLRETTIAKLADKALSEVAQ"
     gene            13173..14642
                     /gene="dnaB"
                     /locus_tag="VIO83_09510"
     CDS             13173..14642
                     /gene="dnaB"
                     /locus_tag="VIO83_09510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY8331548.1"
                     /translation="MSSRRQEVDHLATLYGDQHGLRVPPHSIDAEQSVLGGVMLLNRA
                     AQDVTDLLSERSFYRRDHQLIWRAIMELGEKRQPIDTVTLGEWFESQGSLEMVRDGGY
                     LIELASTVPSAANIRAYAEIVAEKAMLRDLIEAGQTLIDDAYNPDGRSALDLVGTAQS
                     RIGGLLDNEPCDLEPVAPVMARVFEQLAQASTSTAGISGLATSLGDLDQLLDGLLGGR
                     LYVLAARPKMGKTTLAQNIAEQVALRAGRSVAFFSFEMKPEELGKRMLANLAGISGNK
                     LRSGDLDEADWQNVTHWTRKIGDARLRISRPRIAKVQHVCAQVRRMKVQDPDLMLVVI
                     DYLQLMHVSGDNRAAGIGDITRALKLMASELDVAVLLLSQLNRKVEERTGDKRPLVSD
                     LRDSGSIEQDADAVIFIYRDEIYNKDSRYEGTAELIVAIQRDGAPGMARVQYEPGYFR
                     FSNLPEWWRPKDTSASAPPAGKAPVRRGLAAALNAPVGD"
     gene            14645..15112
                     /locus_tag="VIO83_09515"
     CDS             14645..15112
                     /locus_tag="VIO83_09515"
                     /inference="COORDINATES: protein motif:HMM:NF017578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HEY8331549.1"
                     /translation="MLGTFILRAGDARARMAAAWQFACSFLEIGQDVHVTVKQYKPTR
                     SLEQNAMLHAICGEIAQQKQWAGRRIDAEGWKRLLVDAWARESDRRQGDVVPSLDGAS
                     IVNLSMQTRRMTVADMADLITFAQWWATDNDVKLRDVAPLRDQRLAEEGRAAA"
     gene            15109..15483
                     /locus_tag="VIO83_09520"
     CDS             15109..15483
                     /locus_tag="VIO83_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331550.1"
                     /translation="MTPWHIGIFTDTLRRQYPVMANYTAEEDAAARRRYYAGMLCVAP
                     RYLTSAETAKERLKAISKQDREACNDGKWVKRWRDPLAGWDIRMGSTACAPIAYLYGR
                     PVTDPAEVQRYFKPRYRWQVRR"
     gene            15480..15710
                     /locus_tag="VIO83_09525"
     CDS             15480..15710
                     /locus_tag="VIO83_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331551.1"
                     /translation="MSRPGFIPHAQCMVASGECFTQGRCLQKCNPSVLHGLNAEQLLI
                     MAAQAQRKAAGQGSRIASKIDQMIRDVRGGVL"
     gene            15707..16000
                     /locus_tag="VIO83_09530"
     CDS             15707..16000
                     /locus_tag="VIO83_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331552.1"
                     /translation="MRTKNSRAFTKAESAHIELVKLCPCSVCGRAGPSEAHHIVQGDH
                     FTTVALCQDCHRGSENGWHGRKTMWRIYKIDEVGALNETIRNVLLLMSTRGIR"
     gene            15997..16527
                     /locus_tag="VIO83_09535"
     CDS             15997..16527
                     /locus_tag="VIO83_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331553.1"
                     /translation="MTMRLTFGVDPGLTGAVATLIDGEPGPVLDMPTVEVDGWGEVDA
                     RAVVVFIRELRAAHPGAYVSACIEKVGARPGDGGTSAFRFGQTAGKLQAILEVLGIPA
                     TRVVPAQWKRAFLLLKQDKDAARLLAIARFPSAQKILSRKKDNGRADALLIALWHENT
                     QLGSDVMGEQPEEQAA"
     gene            16524..16814
                     /locus_tag="VIO83_09540"
     CDS             16524..16814
                     /locus_tag="VIO83_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331554.1"
                     /translation="MSDIENRARELLAEQYRNGGASAAASEILAGRSSWPNQMALAAI
                     VAAIAPPQGYVLMPVVLPEPMLDVLWSAEADMRDADMQDLWSALIAARPEVP"
     gene            16814..17053
                     /locus_tag="VIO83_09545"
     CDS             16814..17053
                     /locus_tag="VIO83_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331555.1"
                     /translation="MIVQLAIACTGVTAIWLTQSKSERMRRFACLFGLAGQPFWFAAS
                     IAAEQWGIVVLCCFYTVAWSRGVWNNWIARTGQSA"
     gene            17068..17553
                     /locus_tag="VIO83_09550"
     CDS             17068..17553
                     /locus_tag="VIO83_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331556.1"
                     /translation="MATPQPATKQPHRQFNRPTLPLGSVSVRADIHQVSERTLARVKE
                     VRRARGCGTAVFADPDGRVYALRSESVSADTMASKHPEWFVCEYAGRLSSGASAGCPS
                     REDIAEDLVFHFEAMGRVLRDVPEQLDLWGFQGAFAEALCQLARVHRSRRQYMAAAWL
                     R"
     gene            17584..18087
                     /locus_tag="VIO83_09555"
     CDS             17584..18087
                     /locus_tag="VIO83_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331557.1"
                     /translation="MSDRQTPESRLHALTDPHASIVGAGGSVGGIDTRPIPGWQNHAG
                     GATRGVKEYRGDQHAGVDNSVQAAQGCRVAAGVSDGADLCTLGTADLPVVDQSMLDML
                     WERRREVYGRTGKLPTEFTLTPEEFTQLLAEVTPGTSYVVFNSGAEFGYMAFGIPVRV
                     VVSPHGR"
     gene            18077..18559
                     /locus_tag="VIO83_09560"
     CDS             18077..18559
                     /locus_tag="VIO83_09560"
                     /inference="COORDINATES: protein motif:HMM:NF045246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331558.1"
                     /translation="MAGKKPKTTGRPSSFNPEVAERLCMLIAQGDSVAKAAEADGMPD
                     ARTIFRWLATEDTDGTLGFEAFRQQYVRAREIRADARFERLDEIMAMVEAKKLDPAAA
                     RVMMDAIKWQAGKENAKRYGEAVTLKGDKDNPVEIRSPRQLSDADLLAIAAGGLRGAE
                     "
     gene            18546..20147
                     /locus_tag="VIO83_09565"
     CDS             18546..20147
                     /locus_tag="VIO83_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331559.1"
                     /translation="MALSEIRQADAAAELLRRRKARESLIGFAQAISIPGAPVADDRT
                     EWLFKPVESGLAAHHRLILTAVERCIRAPMGRLMLFLPPGSAKSTYASVVTPPWALAQ
                     REGYKVILCSYASTPAERQSRRCRAIVNSEEYASIWPDRVMLKPGSSAVNEWELTNDS
                     GLLAAGILGAVTSARADLLIIDDPVAGREEANSETIRKKTREAYDDDLLTRLKPGASV
                     ILIQTRWHPEDLAGSILPEDYSGQSGPIMCRDGQVWDVLCLPARAERADDPLGRAVGE
                     YLWPEWFPPEHWRQYEQKPRTWSSLYQQRPRPDEGNQFENTWFEWYDEDEVPKQLRIY
                     GASDYAVTKKSLTNDPDYTEHGIFGVDQKGDIWIRDWWYGQDESDVTITAELQLAKRW
                     RPVMTYGESGVIEKAIGPLRARLTRELQVRVMREWLPSVSDKVSRVAGFRARAHARTV
                     HLPRGKAWANRLVDQLVAFNGLPGNQDDGVDVCGLIGRALDHMRDAHKPSVERRDNLV
                     PHTRRWLESRPDDDNDVAARRRNNL"
     gene            20144..22201
                     /locus_tag="VIO83_09570"
     CDS             20144..22201
                     /locus_tag="VIO83_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331560.1"
                     /translation="MTPETNDFAFVDALDADAMAEQERAAEANRILEEEADATAWLKR
                     IEEAREFDKAARKGYAIDRRYCEDKVDPEVYDVSVPIAGTYVNILTGFLYARDPETSV
                     QPAESAGPSRIEDAKMLSRTLEIVIANLWKKGKLKASADQMVRSGLTIGIGWIKAAWH
                     RETERDPATEKQITTVRGQIAQLQATERQLAEGDAPNPDELRARYEQQLAGLEASVEQ
                     VIYSGLCIDFVRGEDMQVAISTPCLKDYTGSPWIAQRIFRPLDKAKADYPEVADRLSS
                     ATLFYNVRPSDADKRSGSVSDVDADAYSSSGSPRTANESSGANLCIWELWNRETGQVL
                     TLAVGLKRYLRAPFTPDQKSTRYYPFFQWAPLWVDGRRHPQSLVDRSRSLLDEYNRTR
                     TNYREHRRRAIPKMGFDAGAVEASEADKMKAGATGEMVPLNLNGASPNTVVFPIQYNQ
                     IDPALYDTSTIRSELELIWGIQEALSSTITVAKTATEADIQQQGTESRIGYARDTLDE
                     MLSELSTYTAEVSMSPNGLTHEDVVAIAGPEAFWVNTEELGLMDSLVAVDIRAGSSGK
                     PATAMKQQQWSVLLPQLQQAVVQIGEMRGSSRQDIADSLEQLVVETIKRTGDTGIDPY
                     SIIPQAPPPPMPVVPGMPGAPGAPGPDGLPLPAANDPAALAMQDPGLQLPPEMLPPQL
                     PAA"
     gene            22214..23320
                     /locus_tag="VIO83_09575"
     CDS             22214..23320
                     /locus_tag="VIO83_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331561.1"
                     /translation="MDPNAETQQAAAPAPDTDQTVPDQVETPSTDKLDAFAAGVEEAR
                     AAETAEVTPAAAPLDDAAAGNPAAAGAAPVEGEGGEPAAAAAPAAGTDPAAAAPDAAA
                     AAAQPAHGKTVDEEIKDLGITNERTQKRFRELSERASEADSLRGRAEKVSDWEQTIQS
                     TGTNPQQFGATLLYLTDINSGDPVRMNRAYETMQAELQFLGEKLGREAPGFDPLTAHP
                     DLAEKVKTGDLDRAAAVELVQHRQRGVLQQEQHQNQQRASQSEQAAQQGLQAVAELGN
                     QLRTSDPQFQQKFAYLAPTVEIIQTTMPPAQWAAAIQQAYQRLPPIPAAAPAVAARPN
                     NPARASAAPAVPATPKNPSDAFSFGVAEAQAQGR"
     gene            23646..24086
                     /locus_tag="VIO83_09580"
     CDS             23646..24086
                     /locus_tag="VIO83_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HEY8331562.1"
                     /translation="MNDKTIEQEIQSKGLTAPRVTPADIEAEIAGEHFFTAGDGYAGR
                     AALTVEEGGVIETPESLDLLTFCVLVLRNGFTVTGESACASPENFDAEIGRKIARQNA
                     VQKIWPLLGFRLRDQLAKEVHGSLQRLLRHQERQRHRQHLGAER"
     gene            24013..24198
                     /locus_tag="VIO83_09585"
     CDS             24013..24198
                     /locus_tag="VIO83_09585"
                     /inference="COORDINATES: protein motif:HMM:NF022642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MW1434 family type I TA system toxin"
                     /protein_id="HEY8331563.1"
                     /translation="MVPYNAYFAIKNVNDTVSTWVPSVNDCLADDWYVLSDVVLTDAD
                     AEADLAGTPRPDHPTAA"
     gene            24409..25470
                     /locus_tag="VIO83_09590"
     CDS             24409..25470
                     /locus_tag="VIO83_09590"
                     /inference="COORDINATES: protein motif:HMM:NF033394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEY8331564.1"
                     /translation="MPLTTAQMLAGANRQMESYATNDPIDQFSTERPFASWLIANKKE
                     STFGNGIFNEKVRIGNDSNYQNYTGDDQVTYNRKDTVRKAPFQHYEAHDGFTLNETEL
                     ANNGIILTDDKNAVMSDAEKIQIVNLLDENWSALKDGFQENWDIEVHLDGSTNPKAVP
                     GLDALVSTTPNVGVIGGIDAATSPYWRNFADMGIATGTAGNLISHMETLWRQTITYGK
                     MGQPNAIFVGALMYDAIQADALKVMSRQITIGANATGGITLDPSTKALSFKGVPVVWD
                     PSFEVIDARLGAITYPWTKRGYFLNSKTLTLRPVQGRWMVKRTPPRVYDRYTHYFGQT
                     ADYGLTTKKRNSNAVFSIA"
     gene            25517..25840
                     /locus_tag="VIO83_09595"
     CDS             25517..25840
                     /locus_tag="VIO83_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331565.1"
                     /translation="MPNIKKIAVPATVSAGAAVALDVTPLLGGEGREGLLYGPAGGLG
                     TGVVQLQGAPKTAAGVAGTYATLMTLNSATTFPVEIPDLPNFIRANVTTAPAAATELT
                     LEGVQ"
     gene            25840..26334
                     /locus_tag="VIO83_09600"
     CDS             25840..26334
                     /locus_tag="VIO83_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331566.1"
                     /translation="MAKTKIPHVLLLIDRDASTKIPVMVPEYEQTILEEIYGEDLVHE
                     VEGQAKDIEVEDFDVQKAFDGLMSKYQATAEGDHARKLIYPKLRDLEKRLKALGVKVA
                     SKDDDGGDDSLAAGTIAQITAALADLSDEELDQLAEDEAAGKDRAGVHAAIEAEREKR
                     TGNQ"
     gene            26406..27143
                     /locus_tag="VIO83_09605"
     CDS             26406..27143
                     /locus_tag="VIO83_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331567.1"
                     /translation="MADPISYNCECDDDYPRTTLLEMRKRLLRRLGFAAQANNPPPGM
                     ADLLNDFITSAQELLFRRYSVMRRERFYTWDLVAGTRFYDLDANADECTKQLDPRMIT
                     WAGLSQGDGNWRPLYSGIDPVMYTARGPGIPSHYEVRQCIELWPAPADATWKLRIKGQ
                     FGLMPLVADSDFTTVDPEALFLLALANAKAHYGQPDAGNYASQLSTYVRDLIRGDHLT
                     RRYIPGTSDPRNAVRPVPVGGWPEDTP"
     gene            27140..27895
                     /locus_tag="VIO83_09610"
     CDS             27140..27895
                     /locus_tag="VIO83_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331568.1"
                     /translation="MRQQSLSTVKAGITRLRDKGGASQDSVYDLVNGYVTAARTIKSR
                     PGTRIAHVLPAGTKGLVFFQGKFVVFANQVTASPSPQVEIEVLRHPTVPGAPIKEIHF
                     SMPFLGYLYVVAEFEGASDPTYVHFWLEKGEVWQPNKTYFPGALVRPTTGNGLAYRLE
                     GDISKYLPWAPNVARAVGDVVVPTIDNGFKYTVTDTTGASARSGTTEPAWPTNAGETV
                     YEDANIPDALGSNTTTTPAVPPNVKDRYGPGRS"
     gene            27895..29319
                     /locus_tag="VIO83_09615"
     CDS             27895..29319
                     /locus_tag="VIO83_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331569.1"
                     /translation="MAVPTWQPGTLYQPGDIVQPITAPPPTAAQVENGDFAAGNVNWD
                     FIGGAAFATSGGYSGNGNCVRMPGNVADGIALNRTKLVVPSTGSTFEASAMINQGASI
                     RGATRGWVEVRWFDIDDAQIGAERGNVVDDGSGGAWHQSKVTATRPAGAAYARAGIGL
                     FSVADHSHPIWGDNLAVSGTFAGLPDGLVYKAVQPESGFSDSDEPAWPPLLGQQVIDN
                     DVIWEAVASTRVTWEASPLYVSGAVEPVWPTDIGGFVRDGTIDWKTVSRRVEDPNCPD
                     SKTVVIAASKVFAGDDDIVRYSATVNPLDWTTADDAGYLPTGLQNYGSNPVAAMGLYR
                     GNLVVFNAEGFQLWQVDEDPASMSLIDALPLGSTQHRSIAPVSNDLLFLASQGVRSIG
                     IAASSTNYQTGDVGMPIDPLVDSLMKEVGPQGTSPKGLYHPAAGQYMLIFRGTGNARP
                     SNLLLQENGDPILQESFDYIHLEA"
     gene            29322..30023
                     /locus_tag="VIO83_09620"
     CDS             29322..30023
                     /locus_tag="VIO83_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPRY domain-containing protein"
                     /protein_id="HEY8331570.1"
                     /translation="MADSPISMLPQSGALTGAELLVLVQGDVTCKATLMQALALLSSR
                     CVWDRNIYNSSMGGVISNSGLTHTATLAGIGNLVVASRAHSAGKRYFEVRMDVIAGAG
                     TPTVGVCQSNSSSAQVGQGPAPSTNRNSDWGLLASSGNKYAAGTSTAYGSALAANDIL
                     MVALDLDADLIWWGKNGAWFAAGNPAAGTGAAFNNVQDFVYPAITNSAGSKVTANFAG
                     PFQFAPPTGFSAWDS"
     gene            30024..30521
                     /locus_tag="VIO83_09625"
     CDS             30024..30521
                     /locus_tag="VIO83_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331571.1"
                     /translation="MKSSTILVYSMSSTGQVGAWSLYTIALNVDEWCIAGDDLYLRSG
                     DYIHVFDEEEVGDETGPGIVGEFQGLIQWPWLDFGQPGVTKSLYGFDMVGIGDVAVEF
                     GYDQSNGGAFTEGYTVPADTVPGMVIPMPISAPSFSVRLRYSGTEQWQWNAFSLYLQD
                     LRGMA"
     gene            30561..31070
                     /locus_tag="VIO83_09630"
     CDS             30561..31070
                     /locus_tag="VIO83_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331572.1"
                     /translation="MQTARLPSNVIPCRPVHLVALAECMRETEQAQLLAVMGTPAYSP
                     DTAAHWLINTWAQSAPFALTVVGRDGMPAAAGGFHPVSPGVWQSWMVGSEQGWAEQWR
                     AMTKATRWLIDRLLETQAHRVQTSATTNREKAIEWFERSLGMRPEGVSRGYGLKGEDI
                     AHFSRMRGE"
     gene            31067..31684
                     /locus_tag="VIO83_09635"
     CDS             31067..31684
                     /locus_tag="VIO83_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331573.1"
                     /translation="MMGGGSNGSATKAAQEESWRQNNIDQAVNQINQIYGGASRQADI
                     NDFLGASRSFYTNELEKQKGVADRSLKFAMARNGLTGGTASIDANRTLGENYQSGILS
                     ADRLAQQAAADLRTADDNSRMNLISQASTGMGLTSGAQQAAQAMQANLQGSRGAMKAD
                     ALGDVFGGLANVYTTSKNMAEERRGNRAYGLLYQPGFGAGAAGGR"
     gene            31681..32127
                     /locus_tag="VIO83_09640"
     CDS             31681..32127
                     /locus_tag="VIO83_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331574.1"
                     /translation="MSLATPIAVLDAMMAAVSHPELADICQLQAAMQAQPQADLPVEH
                     NFLPGQYVRTLRMPAGSLVVGKRHRHRHLLIVTGHVTVRTEAGMVELQGTHIIDSLPG
                     MKRAIYAHADSVLITTHLTDETDLDKIEAYVIMPDDAALEIEGETK"
     gene            32124..32792
                     /locus_tag="VIO83_09645"
     CDS             32124..32792
                     /locus_tag="VIO83_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331575.1"
                     /translation="MTWVATAIAIIGAGVSYHNGEQTKKRQDNELGRQIQQRSLRQQE
                     ADKEVMDTLAERAGNNGEAARSSMLDQYMNQVRAAQGSATSGLRQVGGVSDAYRQSAN
                     DAALGISDYAGNTASLMSRIDAPMRQRQGEAQQNAQLSTALNLIGNRSGSDDFLSQLR
                     LQGIRRNAGLDAFSQIAGAYAGAAGGGGGGASSSTGLSNQANGITAANNAQIFNNSQK
                     AWGY"
     gene            32806..34077
                     /locus_tag="VIO83_09650"
     CDS             32806..34077
                     /locus_tag="VIO83_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331576.1"
                     /translation="MSVLQANSQPPQGWASVGQALAGLGGAGREDAYNRGMAKAAQLD
                     QLVQTARFSREKAMEAEDARKGRGGFAPAAIAAGISEGQAGLLSNALMAGYDPTKVSG
                     YLGATQEQGFRGDAVARALAGDFGAANANLMGVANGPVELASVQGQNLINNRLLEGGG
                     GITTTEQGRAGIAADAARAASSYASANSSNASAARTRQAMSLDRADTMGGGGARGAMK
                     APSGYRWTSDGRLEAIPGGPADNGNAGGAKATGDELKQAFAGKQMAEDAYDFAAAFLG
                     KTPEAVRQMSADEIRKQIKQGGRLTAGPVIGRIPGMSALANADLDAYTSSAAGRQARI
                     NNPTGPVTDADFKAAERSVFSAAKPAGVNADLVYRALMRGAGQQQNAAGQSRPAEVRP
                     TTGQAPAIGSTRGGYRYVGGNPSDRSSWEEL"
BASE COUNT         5990 a        11293 c        11312 g         5543 t
ORIGIN      
        1 ggtttcgcct gagcgccttg tgggttagaa tgcgcacccc cggcagcgat gccggggcat
       61 gcaatgggcc gttagctcag ttggtagagc agaagacttt taatcttttg gtcgatggtt
      121 cgaatccatc acggcccacc atttgcacca aggctttcag cctctcagcg ccgctccgca
      181 atattctgag cgctccgcta tttacttcgt cggactggtc aacgcaccgc ggcgcttcct
      241 cacgtaaatc tctgtcgtgg tcaccgactg gtggcccagc tgctgctgcg ccttgcgaat
      301 gtcgccggcc gaatcggcct tgtccgtgcc cgccttggcg cgcagatcgc ggaactggaa
      361 ttgatccttc gggatgccag cacgctcgcg cgcacggtcg aaacggtagc gcagcgcatc
      421 cttgccgatc ggcagccagt cctcaccaag cagcagccgc gtgctgatcg gcgcatcgtc
      481 cggccggtcg cggttcacca gcctgtcgat cagcaacttc agctcgccgg tgatggagat
      541 gcgcatgggc gtcttcgtct ttccctgccg cagcaccagc gccccgtctt tgatgttccg
      601 gcggtccagc tcgcgcacgt cggccggtcg ctggccggtc aggtaggcaa ggtccatcgc
      661 atcgcgcagc acctggtcgg cggcatcgta gacctgctgc agcacatcgt cctcgacata
      721 gacgtcgcgc ccggactcgc ggttgcggtt gatgcccagg caggggttgg gcagatcggt
      781 gtagcccttc tgccggcacc agttccacag gtgtgacagc agcgacttct cacggttggc
      841 gctgaccggc gcgtctttgc gcgacagcag gtagttgtgc acgtgtcgcg gcttgatgga
      901 ctcgagcggg cagggcgggt catcgaagta gcgtttcagc atcgacagct cgcgcatgtt
      961 gaccgcctgc gtgccgggcg ccttcttcgg gatgacttcg gcctggtagc gctcggctgc
     1021 ctgccggaac gtcagcgccg cgcctgcagg gatcgcgccg gcgtgctcca gctccgccca
     1081 cttcttgatg gccaggccat agtcactgcc cagcggcgtc tctttgcgcg gcttgccgcc
     1141 gtggtcgtag tagaagtgca ctacgccgga cttctgcttg cgcttgcgga accgggggat
     1201 cgcccccggc ttggtcggtt tccttcccat catgctgcct tgttggagtt ccattcggcc
     1261 gtcggcgctg ccttcggctc ggttttgccc tcgagcgcgg accacagcac cactggccag
     1321 ccgtaggcgt cgagatagtg ccggatcccg ttcgtgcgca ggaaggcggc ttgccgcttc
     1381 cgctggggcg tgcggcacag gtcgcggata tcgtcgcggg acaggtaggg gctgtcggcc
     1441 atgttgatta tctccggtca gttcgtggcc agggcagcgc gcagctgctc cgtgacctgg
     1501 tgcgcggcga acaggtcggt gggcatgaca acctgcgtcc tgcgcgcggc tttcgcttcg
     1561 aggatgtgcc ccccccactg ctgggccatc gccgcggcta tggccgggtc gaagcgcgaa
     1621 cgctccttct cgcggtccgg gccaggcggc atcaggtggc agcgggcttc aatcttcccg
     1681 tgcacggcaa tcacttcggc cttcgtgtgg gtaggcacca gcgccggcag gttcttcagc
     1741 cagagggtgg cgcccttggt gaaggggcta ccgaagtcgt acggctgcac cgtctgcgtc
     1801 ggtcgaccca gcacgctcat ggccagcccg tgcggcttcg agttctcgtt gccgatgaac
     1861 tcgatcggcg cggccatcag gtcctgaaac agcagagcgc cttcgaggaa gtcgcgcatg
     1921 cggttcggga acttcggatg ccggcgccga tctgcctgcg gcagcgcggt gtcgtcgggg
     1981 tgatacatcc aacggatccc ggccaaggtg ttgtaggtgc aataaggatg ggcaatcatc
     2041 gccagccagc ggccggcctg caggtgattc cgcacgtcgt cctggatatg ccagcgcggg
     2101 tctccctcgg tggggcgcag gtcgcacgaa taggcatcga aaccgcgggc gcggaacgcc
     2161 gacgcgaccg tgtcgctgta ctcgcaggca accaagatgg gcagcagctc agccatgggc
     2221 caccagcctt gccgcgcgtc gccagcacca gcgcaccgcg cggcagaagg cgacgaccag
     2281 ccacacggtc aggaagccca gcaccagcat cagggccagc accaggtact tgcccagcca
     2341 gagggtcagg gcgtcagaca ttggtcggct cctttgcctc acgcacctgc agccgggcgt
     2401 gtagctcctt cacgaagtcc atgccgacgc cgttgatgtt gcacccggcg atagctgcca
     2461 gggctgacat tgcgaggtca tcggaggtcg cgagcggcac cccaggtatc accgtcacaa
     2521 cgctcttggg gtcgtcggct tccacggcga ggatgccggc gtccgccatg cgtttgcggt
     2581 cggcggtaga cagctggccg cgtgggaaca cgatgatctg gctcacgccc ccacccccgg
     2641 cgctgcatcg cgctggccgc tgctcagggc gcggacaatc tcccgcttcg ccagcacgat
     2701 ggcctgattc cggttggtgg catcgatgca agccctggcg tccaccccga tggcctgcat
     2761 gtcgctggaa gtgagggtgc gcgccacgtc gcgcgcatcg aacacatgcc cgccaagctg
     2821 ctcctgccag ttccacatga tgtccagcgc acgccccagg tcgatgccct gcgcggccgc
     2881 tgggtcaagt cgatcaggga agcctacccg ttccgacact tcgttgaact tgcgaaccag
     2941 cgccgcgccc aggtcaatgc cgcgctcggc gcagaacagg tcgagatagg tgacaacgtc
     3001 ggcggcttca ttcgccatgc ggcgagtgcg ttcctccggg gtgatctgtt ccttgttccc
     3061 ggccaggccg tcgcgcacgc ggttgtgcat cttcacctcg ctggccaact cgccaagctc
     3121 gcccatggcg gcagtcagcc agtcagatgg cgaccacgac ccgatcccct cggggtgcca
     3181 cttctcgcac ctggatacgt tggcgttgcg gaacgcggag aaggtgaggg caggcatctg
     3241 cgtccccggc tggcgggcgg cgagggcggc ttgccatgcg agccacgcgg agatggttcg
     3301 ggcgccgttg tactcaccgt cgcgtgcaga tttatgcagg ctcaaggact cgctatgcgc
     3361 ccacgcctca aactgcgtcc gcgcatcccc ctgcacctcg ccaacctgca tgccctgcgc
     3421 attcgtgctc gacctgcaca tttccggcag acctgcactt tcctcgccaa cctgctgctt
     3481 gccagttgcg gcgagcgcgg ctttggcgaa cgtgcgcatc ttcgactcgg agaacagcgg
     3541 ctccccggtg cgcgggtcat gcgccgagga tgctggcagc gacggcagtt ccatcccccg
     3601 ctgctcggcc agctctgcgg gctgcggcgc ggttgcgccg ctcaaagcgc gcgacagcca
     3661 gcggatccgc tccttcgccg ccgtcaggta gacgatcccc gacagcgctt tgccggccac
     3721 aaggtcggcc atggagggct gctcgttgcc gtgcaggtac acggcgttgg ccagctcgtc
     3781 gtcggtcagg tgccccagcg cgagggcggc acgctcgcag gcgtagcggt cgccgtgcgg
     3841 gtccgattgt ccgtctgcgg cccagcggga cgactgggtt ccgggcaggg gctgcagcag
     3901 cggcagcgcc gcgctcacag cagtggcaag cgtccggcgc agcatggtgc cgctgacctg
     3961 ccggaacccg ccttcctcca ggtcgcaggc gatggcgttg acgaacttct cggtgcgttc
     4021 ggtgttcatg cggtcactcc catggtggcc aggtcgatct cgtccacgcg gtcgcgcagg
     4081 cggtggcagg cggcgcgcag ggccagtgca acggcgcggc gctggtcctg cgggtagaag
     4141 ttcaggttca cgatgtggat ggcgccggcg tggtcgcgcc gggacaggcg ataggcggtg
     4201 atgccggtgt cgcccatcgg gaagcggccc cagctgaagc cgccgttgcg cttcggtgcg
     4261 cggcgggtga tggggcggct catggggtca cctgcttgaa gtcgatgacc catacccagg
     4321 ggttgctgtc ccagtccaaa ccctgctcgg cgaaatgggc gcgcatggcg tggtcctcgg
     4381 gcgtaccgcg catactcacg ccagcaatcc agtagtgctc ggcctcgctg aaggcgcgcc
     4441 cggcggtggt gtccggatct tcgatgcaga agctgatgcc ctccgactcc cagtcaccac
     4501 tcggcagagc ttgcagccgc tccacgcgga cggaggtgat ttccagcacc aggcggcagg
     4561 cctcgcgcgg catgtgcacg ctgggcttcc actccttcgg catgcccagg tggtcaccca
     4621 tgcggtgacc gtgctggtag gtagcgcggt agtccacctc gatcaagcgg ccgtcggtgg
     4681 tcgagtagat agggctgaac gactcccgca cccacagccg gtcgcctggc tggccgaagg
     4741 ggcagcccgc agggatgggc accaggccat cggcggatgt ctcccagtac ccatgcggca
     4801 tcttcaggac gcgtcgggtc tgggtcttct gtccggccaa gatggcgcgc accatggcgc
     4861 cgttgaacag gatgggacgt tccttgctca tgcgcctgct cccttcttcg cggccttgcg
     4921 ccgctctgcg ccaccctcga ccacctggcg gcggctgatg ccgctccggt cgataggggt
     4981 gttgcccagg cgctggatcc tgccgccctt gcgctcgaac tcggccacgt cggcggccag
     5041 ctgctcggcc tcggcggcct tctgccgcgc ggtggcggcg tcgaagacgg tctgtgcgtg
     5101 gatgctggtc atgcggtgcg ctccagctcg gccagcaggg cgtctgcctg agcgattgcc
     5161 cagcgagcct taagctcgcc aacggcctga gggctatccc aattgaacgg gccgtggtcc
     5221 gcgttcggga cccaattgcc cattccctgc atcgccatgg cggcgatcag ctcgcgcttg
     5281 gtcagctggc atgccgagta gtttgcgttg ctcacggtgc gtacctcaga aggttggtgg
     5341 gctgcgccgg gtagaaccac agcgaggccg tgttgttgag ctggacctgc accgggtcag
     5401 gctttagcgc cggcagtggc gtgcgcaacc ggcgcgcgtc gttggtgcac atggcgcagg
     5461 tgggttcctt gccgcgccgc ccggcgctgg ggaaggcttc gatgggcagc cggcggcagc
     5521 agcccgtgca ggccttgcgc gcgctcatga cttcaccagc cgggactgct gcagcgtcga
     5581 ccaggtcagc gggtaggggc cgcgcttgat ccgcttggcc gcgccgttga gcgacaggcc
     5641 cagctgcgtg gccacctgct tggtggtgta gcgcttgccg tcgaccacgt gggcgaacag
     5701 cgcggcgcgg gcgttgccgg cgcgcacgca gctcttgcgg tggtaggggt agatgcggac
     5761 gtccatcagg cggccaccgg caggtcgaac agccgacgcg ctgcggcgta ctcgccgagg
     5821 aagcgatcga ggtattcctg cagcttgtcg gtgaagccgt cgcgggtgac gcgcacgacc
     5881 agcggggcgt agccggggca gtaggacatg aagtcccacc actcgcgctc agtgatgatc
     5941 aggcagccgt gcacctgcgg tttgtgttcg tcagggacgc cgccggcgcg cagccacttc
     6001 acgtgtgtgg ggccgtccgg acacttgatc tccaggccac cgggcttgcc gatcaggctg
     6061 tccgggctgc agcccagcgt gccggcgtcg ttgaggatga aaccgacctg ctgcggcacc
     6121 acgtcgtgct cgaaggcata gagctcgcgc gcatcgtctt ccagcagcac gccgcgctcg
     6181 gtgtgccgat tgccggtgaa agcctggcca gcatccgggc gcacggcctc atcgatcagc
     6241 tggttgatgt aggtatcggc ggcagcggca tactcccgct tcttcggggt gatgatgctg
     6301 ccgaactcgc tggcggtcgg cacgccgcgg cgggcggcgt accattcggg cgatcgctgt
     6361 tcgcaggtca ggatccgcat ggctcagtcc ttgggcttgg tggcttcggc ggcgcggttg
     6421 aaggcggcgc gcacttccgg cggcagattc gatgccttgc cgccgtaggc ctgcaggacc
     6481 tcgcccttgc gcgcggtgta ttcctccagg gtcttcaacc cttcggccag ccgcacccag
     6541 tcctgagcga tctcgcgggc gatgcgctgg gcttcgttct cgccggcacc gttgccgtcg
     6601 tcgtcttccc cgccgatcgt caggttgaag atcatcagga tcaggtagcg ccggccgtag
     6661 ctgatcgccg aaccgcgccc gtgggtgctg gtcttgttct tctcgccctt gatgccgacg
     6721 tcatcgatcg gggcgtcgta gtgataggtg cggctgtggc cgccggtgtg cagcacgtcg
     6781 cacacgatgc ccacgtggcc ctgcaggtcg gacgtcaccg tgccgaagga cagcgagaag
     6841 ccctcgcggg tgtagaccgg cgtgatcgcc tggttgatat cttccagcag cgcgtactgg
     6901 ctgttggtct gcttgttctt gccgcgcttg gcgatgttcg gcatggacga ctgggcgcgc
     6961 tgcatggccg tcgcgtactc gcgggctgct tcgcggtcct gcatgtcctt gtgcatttcc
     7021 cacatgcgct ccaggcgctc gacgccctgt tcggggagca tcagtgcttg ctgcagcgcc
     7081 gcctgcaggc cggtcccggc ctggctgctg cctgcgatgg tggtgatctc gttcatcggg
     7141 aattgtctct acatgaaatg aggtgcccgg ctttcggagc cgctgccggg cgagagcgac
     7201 tcaggcggga atggcgtttt cgggcagcag cccgaccttg gccagcagct cgtcggcatc
     7261 gccgggcatc tcctccggcg cggccggcac ggtgaactcg gcgctggaga gcagttcaat
     7321 tgcagccgcg acagagaaag gctggtcggt ttccaagagc acgcgctcgc cgtactgccc
     7381 gccaattgct gcagccaggt cgtcccacga ctcgagggag acaccgtggc agcacagtgc
     7441 gatgcgcaac aggtcgccgc ggccgagctt gtagccgcgc tcgcggtact tgatcgtgcg
     7501 cagcagggag ccgaatggat agcgcgtgcc gctgtggaaa ctcaggtgcc gctgggcgtt
     7561 gtgtttgaag aagtccggat ggaagacgaa cgtctccctg tcgacgtcat aggccgccat
     7621 gcaggcggtg aagtcgaagg cactgaaaat ggcttcagcg gtatcgaaga agtcgaacag
     7681 catcagctgg atgatgtcct cgccgtacgc gaaggtgacc gcgcgatctg ttgccgctac
     7741 acaccaaagg ccttcgtcat aggcggcgcg cactgcatcc atgaacgcct ccttggtttt
     7801 gaagtagaga tccacatcgt tgatcggctg gccggtgaaa atgctggtga gcgctccgcc
     7861 ggcaatgaat gagttcttgg gggtgaagtg ggctgcagcg gtcagcagct tcttcagttc
     7921 gggtgcatgg gcagtcatca gaagtcctat tcgcctgtgc tggcggagtg ggtaggggtg
     7981 ccggcattgg ggaacccggc cggcgcgggt ggtgctgccc gtggggagcg ggcagcgggg
     8041 gattaggacc aggtggccgg ccagcacacc gcgcacgcga gcgcggcggt gatgccgtag
     8101 caggcaaggc aggcggcgaa gtcggaccag ctgcggcatc cggtggcggt caggaggcgc
     8161 atcaggcggc acccgtggct ttggcgatga cggcgctggt atcgaagtca gcgcaggtgt
     8221 tgccgagcag gcgcatgtcc gcgctcatct gatcggcggc gttgtgtgca ccctcgggat
     8281 accagtcagc aactcggcgc tgatgcagtt cctggcggat gctgtcaatg aactcctgtg
     8341 cacgctgcag ctcggccagc agctccggcg cggcggcgat caggcgggcg ttggccacgc
     8401 gcgtggcttc ctctacgccg tgcggggtgc tgactggcag ctgataggta acgctgcaga
     8461 tcgtcaggcc gtcttcggtt gtcacgtggt gcccgtactg gtacggatca gtggggtggt
     8521 cgtgaactgc ccacggcccc ggcgtgtgct tactgctcat cgttcgaatc ctctgcgcag
     8581 aggccgttga tggcctcgtg gtgggtcatg tgggcctcgg ccagcgcggc gtcggcgagg
     8641 gatacgggcg gggtgatcag cggaaacggc tgtgcgaaca gccgcgcgag ctcggagcag
     8701 ggcgtcatga ccgcgcgccc tcggcgtacg cagccgcagc cttggcctgg ctcaccgcct
     8761 gccgcgcgtt gatcgctgcg acgtgctgct ggatctcccg gctgacggtc tgcacctgct
     8821 gcaccggcac cgattcgaac tccacgtact cgatggcgat gcccaggtgg ttggtcttct
     8881 cgatcaggtt catgcgatcg ctcccaccac cagggcgaac gcagcgccca ggcagaacgc
     8941 gatgaagtag atggcgtagc ggtgcaccgt cgggccggtc attgccagat ccccgtggcg
     9001 acggcgccaa gccacagcag gcccatggcg cagatgccgc agccgacgat ggctgcgaag
     9061 tccttggtgg cttcgatcac ggcggtcatg cggcaccgcc ttcactgtcg gcagcgatcg
     9121 cggtaggccg gttgaaggtc accctttcca cgagggtgtg acccagccgg tagctatcgc
     9181 cgaacagagt tgttcccata aatccgccac gcttgatgtc agagcggccc accgtgcgga
     9241 ggcatccgtt atgcgacacg gtgtccccgg cacggatttc actgatgtgc acgggctccg
     9301 tggtggtgat ggtgggcgcg ctcatgccgc cacctgctgc agcgccgctt ccagcagctg
     9361 ctcgtccagc gccgcagcct gcaccgcagc cagcgcgtac agctgggcca gcagcggcga
     9421 gtcgatcagg tccgaagggt ccgtgtggtg cagctggtgc agcgccaggg tcacgtccgt
     9481 gtagtggtca ccgtcgaagg tgcccgccac ccactgctcg gcctcgcggc acgctgccgg
     9541 gtccgagcgc atggcctcgg ccagctcggc cgcctgctgc ccggccagtt cggattcaaa
     9601 gcgcggatct tccaggctgt cccagctgcg ctgggcgttg cggaagttgt cgttgagagt
     9661 tgccctttgc atctttgtgt atcccatccg cgccaatcgg ccgatgggga tagattacca
     9721 aacggtaatc gcacgtcaat accaaaaggt aatttacgac gcgagaaaaa tgaacggaca
     9781 gcgagaccgt tcatgtttgg attcagtagg ggatgcgccc gtccagatcg atcgccttcg
     9841 tctccacgct cccgagcctg ccttccaaat cctctatctc ggactgctgc tgcctgttct
     9901 cttgctccag gctgtctacc ctgctttgta gcgcctcgac ctcatctccc agagcttccg
     9961 cggcgctgct cgccgattgt gctgcggcca ttgcgtcccg cgccacatcg agggcttgtg
    10021 tgtcttcttg agccgagcat gcggagagca gcagaagtgc tgttgccgaa aaaatcagct
    10081 tcattccgtg agcctctcag aaacgccgga gcccggcatg gataagcgct ttgcccagga
    10141 tcgccacgtc gctggcctcg gaccgatagg cggggaactc agggttcacg ctcaccacgt
    10201 agagaccgtc gccgcgcttc tggagcattt tgatctgggt ctcaccaccg atgttgatga
    10261 ggtaaaagtc gtcgccatcg aagtagtcgc agctggtgtc gatccatacg atgtcaccgt
    10321 cttctagctt gggacgcatc gacgggccgc ggccggtgat cagctgtatg cggccgggct
    10381 tgggaagaaa tccgagcttc cggcggacct cccactccgc caccgcgatt gtcttgacga
    10441 cttccggata gtcctggtta accaacccct gtcccattcc cgcaccccct tcgaacaagt
    10501 cgaagcgaac atagccgggc gccgtctcag ccgataagac ctcttgtggc gcatcggaag
    10561 acaggtagcc gtctggcatc cccgctgcct tttccagcgc ggcggctcgc ttctcgccaa
    10621 acgatttatc gcggagcagt gacgaaagct ctccttgatt gacaccagtg gccagaacaa
    10681 aagacgcctg actgccgtcg tgcctgtcgt ctatccactg tttcagtcgt ctgcggcgga
    10741 gggccacggc gggagtgtcg gtcttcatct cccgattctc agttaccgaa gggtaattta
    10801 ccaaacggta ttgacttgtc tttaccatat ggtaatttcc ttggcatgga cacccttcgc
    10861 acgtacctct cgaccctcac cccggcagag caggcggcgt acgccgttcg ggccggtacc
    10921 tccatcggct atctgcggaa agccctgagc aaagggcagc gcttcgatgg cggcctggtg
    10981 cgtctgctgc acgtcgagag cgacggccag gtttcgctta ccgaactccg tccggacatc
    11041 tggcccgaaa acgccacaga cgctgttgcc tgacatgcgc acctcaccgg ccgccggtgg
    11101 ggaagggcac cacggtgccg ctgccgatca ccacgctgcg gcgccgctgg gccacaggcc
    11161 gcaccgacac cttccgtccc acccgcttca ggacgaagca cctaccgcca caccgcacga
    11221 gcgtgaggac gttggcctcg gctgactgat cacttttcga caccggctgc actccgttgg
    11281 ggtgcggcca ttttcagaac caccactgat aaccgctgat aacagatcag ctcggacctt
    11341 atcaatgacg cagataactc agcagaaagt aagggaattc ctcgtgtatg agcctgatac
    11401 aggcctgttt cgctaccgca gcggaactcg tgccgggcag gtttccggcc acaagcatca
    11461 cggctacatc aagatcagca tccccggcca cggtggcatt tacgctcatc ggctggcgtg
    11521 gctctacatg acgggggaaa tgcccccgat gttcatcgac cacatcgact gtgttcgcga
    11581 caacaacgaa tgggcgaatc tgcgattggc tgatcgcagt cagaacagcg cgaacgccaa
    11641 gatcaagaag acgaacacct ccggattcaa aggtgtgcgg ctttttgggg gcatcttccg
    11701 cgcgaagatc accgtgcagc ggcggatcat ccacctgggc gactacgaaa ccctggaaga
    11761 ggccgtggct gcctacaagg gtgcctcgcg agtgggcttc ggcgaatacg cgaggacccg
    11821 ctaatgcagc agctcaatat ttcctttgag gtcgggctgg atcggcgcta ccgcgacatg
    11881 cgcgagtgct tcgcctcctg cgtctattcc aagggcttgg ggcgggtggc cgccgccatc
    11941 gacgtagctc cgtccaacct ctccgcgatg cttgccgggg accgcaacct ggacagcacg
    12001 ctggtcgaga agtacatggc agagttcggc gacaccacgc cggccatgta ctgggccgcg
    12061 aagcacctcc agtgcgccac gactcggcaa caggccgccc tggcgcagct cccgtcactg
    12121 gttgaccagc tcaatcgcct gatggcagaa gccgggaaga cgccatgaac gcgaccgaga
    12181 aggccatgct cgccgtccgc gtgctgtggt tcaccgcggc gtgcctgcag ctgctgggag
    12241 gccgccatgc gtgattacgc gaagatcgtc ccgacgttct ggaccggcga caccggcaag
    12301 gcaatccgca agaggggggc tgaaggggtg gttgtcgccc tgtacctgat gtcctcgccg
    12361 acttcgaaca tgttgggtct gtactaccag cccgtcctct acatggcaca cgagaccggc
    12421 ctgggcatcg gaggggcatc agaggggctt cgctactgca tcgaagcggg tttctgccag
    12481 tacgacgagg tcaccgagtt cgtatgggtg cccgagatgg cagcgttcca gattggcgag
    12541 ggtttgaagg ccaacgacaa ccgctgcgtg ggtatccaga aggactacaa cgggcttccg
    12601 gacaacccgt tcctgggcga cttcttcgac cggtatcagg ccgatttcca cctgcgcaat
    12661 cgacgcggcg gggaaggggt tgcgaagccc ctttcaagcc aagaacagga acaggaacag
    12721 gagcaagagc aggaaagaac atcttcgctt cgctcagatt cgtccccgca gctggcgctg
    12781 acgggcacca cgccggcccc tgctgaccac aagccgtcca gggcagagcg aatccgccag
    12841 atcgccggag acgcccaggc tgcgttcaac gccacgctgg ccaagccggt cgggctgctg
    12901 gcgaagtgca ccgtgctgaa cggccctcgg atcaaggccg tggagaaggc gctgccgacc
    12961 gtgcgccaga tctgcctgca gctgttcggc agcgagcggg tgaccccgca gttctggcag
    13021 ctgtacttcg agacggcggc cgatgacgac ttctacgccg gccgggtgaa gggcgggcag
    13081 ggccacgaga actacgtgcc tgacttcgcg ctgctgctgc gcgagacgac catcgcgaag
    13141 ctggccgaca aggcgctgtc tgaggtggcc cagtgagctc gcgccggcag gaagtcgacc
    13201 acctggctac gctgtacggc gatcagcacg gcctgcgcgt gcccccgcac agcatcgatg
    13261 cggagcagtc cgtactgggc ggggtgatgc tgctgaaccg cgcggcgcag gacgtgaccg
    13321 acctgctgtc ggagcgttcg ttctaccgcc gcgaccacca gctgatctgg cgcgcgatca
    13381 tggaactggg cgagaagcgc cagcccatcg acacggtcac gcttggcgag tggttcgagt
    13441 cgcagggcag cttggagatg gtgcgcgacg gcggctacct gatcgagctg gccagcaccg
    13501 taccatcggc ggcgaacatc cgcgcctacg ccgagatcgt ggccgagaag gcgatgctgc
    13561 gcgacctgat cgaggccggg cagaccttga tcgatgacgc ctacaacccc gacggccgca
    13621 gcgccctcga cctggtgggg accgcacaga gccgcatcgg cggtctgctg gacaacgagc
    13681 cgtgcgacct ggagccggtg gcgccggtta tggcccgggt gttcgagcag ctggcgcagg
    13741 cctccaccag cactgcgggc atcagcgggc tggccaccag cctgggggat cttgaccagc
    13801 tgctggacgg cctgctgggc ggccgactgt acgtgctggc ggcccggccc aagatgggca
    13861 agaccaccct ggcgcagaac atcgccgagc aggtggcgct gcgcgctggc aggtcggtgg
    13921 cgttcttcag cttcgagatg aagcctgaag agctgggcaa gcggatgctg gccaacctcg
    13981 ccggcatcag cggcaacaag ctgcgcagcg gcgacctgga cgaggccgac tggcagaacg
    14041 tcacccactg gacacggaag atcggcgacg cccgcctgcg catcagccga ccgcgcatcg
    14101 ccaaggtgca gcacgtgtgc gcgcaggtgc gccggatgaa ggtccaggac ccggacctga
    14161 tgctggtggt gatcgactac ctgcagctga tgcacgtgtc cggcgacaac cgcgcggccg
    14221 gcatcggcga tatcacccgc gccctgaaac tgatggccag cgagctggat gtggcggtgc
    14281 tgctgctgag ccagctgaac cgcaaggtcg aggagcgcac cggtgacaag cgcccgctgg
    14341 tatcggacct gcgcgactcc ggctcgatcg agcaggacgc ggacgcggtg atcttcatct
    14401 accgcgacga gatctacaac aaggacagcc gctacgaagg caccgccgag ctgatcgtgg
    14461 cgatccagcg tgacggcgca cccggcatgg cccgcgtgca gtacgagccg ggttacttcc
    14521 ggttctcgaa cctgcccgag tggtggcgac ccaaggacac cagcgccagc gcaccgccgg
    14581 ccggcaaagc acccgttcgc cgcggcctgg ccgcggccct caatgcccca gtgggagact
    14641 gaccatgctc ggaaccttca tcctccgcgc cggcgatgcg cgcgcccgaa tggctgcggc
    14701 ctggcagttc gcctgcagct tcctggagat cggccaggac gtgcacgtca ccgtgaagca
    14761 gtacaagccg acccgcagcc tggagcagaa cgcgatgctc cacgccatct gcggcgagat
    14821 cgcccagcag aagcagtggg ccggccgccg catcgatgcc gagggctgga agcggctgct
    14881 ggtggacgcc tgggcccgcg agagcgaccg ccggcagggc gacgtggtgc cctcgctgga
    14941 cggcgccagc atcgtgaacc tgtccatgca gacccggcgc atgaccgtgg ccgacatggc
    15001 cgacttgatc accttcgccc agtggtgggc caccgacaac gacgtgaagc tgcgggacgt
    15061 ggcaccgctg cgcgatcagc ggctggccga agaggggagg gctgcagcat gacgccctgg
    15121 cacattggaa tcttcacaga cacgctccgc cggcaatacc cggtgatggc caactacacg
    15181 gccgaggaag atgctgcagc gaggcgtcgg tactacgcag ggatgctctg tgtcgcacct
    15241 cgctatctaa cctccgccga gactgctaag gagcgtttga aggcgatttc caagcaggac
    15301 cgcgaggcct gcaacgacgg gaagtgggtg aagcggtggc gcgatccgct ggccggctgg
    15361 gatatccgca tgggttccac ggcgtgcgca cccattgcct acctatacgg ccggcccgtt
    15421 acagacccgg ctgaggtcca gcgctacttc aaaccgcgct accgttggca ggtgcgccga
    15481 tgagccgccc gggattcatt ccgcacgctc agtgcatggt cgccagcggc gagtgcttca
    15541 cccagggtcg ctgcctgcag aagtgcaacc ccagcgtcct gcacgggctc aatgccgagc
    15601 agctgctgat catggcggca caggcacagc gcaaggcagc cgggcagggc tcccggatcg
    15661 catcgaagat cgaccagatg atccgcgacg tgcgcggggg tgtcctttga ggacgaagaa
    15721 ttccagggcc ttcaccaagg ccgagagcgc gcacatcgag ctggtgaagc tctgcccgtg
    15781 cagcgtctgc ggccgagccg gacccagcga agcccaccac atcgtgcagg gcgaccactt
    15841 caccaccgtg gccctgtgcc aggactgcca ccgcggcagc gagaacggct ggcacggccg
    15901 gaagaccatg tggcgcatct acaagatcga tgaggtcggc gccctgaacg aaaccatccg
    15961 caacgtactg ctcctgatga gcacaagggg aatccgatga ccatgcgact cacgttcggg
    16021 gtagaccccg gcctcaccgg cgcggtggcc acgctgatcg acggcgagcc cgggccggtg
    16081 ctggacatgc ccaccgtcga ggtggatggg tggggagagg tggatgcccg cgcggtggtg
    16141 gtcttcatcc gcgaactgcg ggccgcgcat cccggcgcct acgtgtcggc ctgcatcgag
    16201 aaggtcggcg cgcgcccggg cgacggcggc accagcgcat tccggttcgg gcagaccgcc
    16261 gggaagctgc aggcgatcct ggaggtgctg ggcatccccg ccacccgcgt ggtgccggcg
    16321 cagtggaagc gcgccttcct cctgctcaag caggacaagg acgccgcgcg cctcctggcc
    16381 atcgcccgct tccccagcgc gcagaagatc ctgagccgga agaaggacaa cggccgcgcc
    16441 gacgcgctgc ttatcgccct gtggcacgag aacacccagc tcggcagcga cgtgatgggc
    16501 gagcagcccg aggagcaggc ggcatgagcg acatcgagaa tagggcgcgg gagctgctgg
    16561 ccgagcagta tcggaatgga ggcgctagcg ccgcagcctc cgagatattg gctggtcgat
    16621 ccagctggcc gaaccagatg gctcttgccg ccatcgtcgc cgcgatcgcg ccgccccagg
    16681 gctacgtgct gatgccggtg gtgctgcccg agcccatgct ggacgtcctg tggagcgccg
    16741 aagcggatat gagagacgcg gatatgcagg acctatggtc tgcgctgatc gccgctcgcc
    16801 cggaggtgcc gtgatgatcg tccagctcgc cattgcctgc accggcgtca ccgccatttg
    16861 gctgacgcag tcgaagagcg agcgcatgcg ccgcttcgcc tgcctgttcg gcttggccgg
    16921 tcagcccttc tggttcgccg cgtccatcgc cgccgagcaa tgggggatcg tggtgctgtg
    16981 ctgcttctac accgtggcgt ggtcgcgcgg ggtctggaac aactggattg cccgcactgg
    17041 gcaatcggcc tgatcccgac gaccgccatg gccaccccgc agccagccac caagcagccg
    17101 caccggcagt tcaaccggcc gacgctgccg ctcggctcgg tttccgtgcg cgccgacatc
    17161 caccaggtct ccgagcggac cctggcccgg gtgaaggagg tccggcgcgc gcgcggctgc
    17221 ggcacggcgg tgttcgctga tccagacggc agggtctacg ccctgcgcag cgagtcggtg
    17281 agcgcggaca ccatggccag caagcacccg gagtggttcg tctgcgagta cgccgggcgg
    17341 ctgtccagcg gcgccagcgc gggctgcccc agccgggagg acatcgccga ggatctggtg
    17401 ttccacttcg aggccatggg ccgggtgctg cgcgacgtgc ccgagcagct ggacctgtgg
    17461 ggcttccaag gggctttcgc cgaggcgctg tgccagctgg cccgggtgca tcgcagccgc
    17521 cggcagtaca tggcagcggc ctggctccgt tgatccaact gaccgctggc gcgtaatagg
    17581 cccatgagcg accgccagac gcccgaatca cgactgcatg ccctgaccga cccgcacgcc
    17641 tcgatcgttg gggccggtgg gagcgtcgga ggaatcgata cccggccgat ccctggctgg
    17701 cagaaccacg caggaggcgc cacccggggc gtcaaagagt accggggcga ccagcacgca
    17761 ggtgtcgata acagcgtgca ggcagcacag ggttgccgcg tcgcggcggg cgtttcggat
    17821 ggcgccgacc tttgcaccct gggaactgcc gatttgcccg tggttgatca gagcatgctg
    17881 gacatgctgt gggaacgccg cagggaggtg tacgggagga ctggcaagct gccgaccgag
    17941 ttcaccctca cgccagaaga gttcacccag cttctcgctg aggttacgcc cggcacgtcc
    18001 tacgtcgtgt tcaattcagg ggccgagttc ggctacatgg cgttcggcat ccctgttcgc
    18061 gtagtggtct ctccccatgg ccggtaagaa gcccaagacc accggccgcc cgtccagctt
    18121 caacccggaa gtggcagagc gcctgtgcat gctgatcgcc caaggggaca gcgtagcgaa
    18181 ggcagctgag gcagatggca tgcccgatgc ccggaccatc ttccgctggc tggccacaga
    18241 agacacagat gggacgctgg gcttcgaggc gttccgacag caatacgtgc gcgcacgcga
    18301 gattcgagcc gatgcccggt tcgagcggct ggacgagatc atggccatgg tcgaggccaa
    18361 gaagctggac cctgcagcgg cccgggtgat gatggacgcc atcaagtggc aggccggcaa
    18421 ggagaacgcc aagcgctatg gcgaggccgt gaccttgaag ggcgacaagg acaatccggt
    18481 cgagattcga agcccgcgcc agctgagcga tgccgacctg ctggcgattg ccgcaggggg
    18541 cctccgtggc gctgagtgag attcggcagg ctgacgcggc agccgagctg ctgcgccggc
    18601 ggaaggcccg tgaatccctg attggcttcg cccaggcgat cagcatccct ggcgcgccgg
    18661 tggccgatga ccgcaccgag tggctgttca agccggtgga gtctggcctg gccgcccacc
    18721 accgcctgat cctcacggct gtcgagcgct gcatccgcgc gccgatgggc cgcctcatgc
    18781 tgttcctgcc gcccggctcg gcgaagtcca cctacgccag cgtggtgacc ccgccgtggg
    18841 cactggccca gcgagagggc tacaaggtca tcctgtgcag ctacgcgtcg acgccggccg
    18901 agcggcagtc ccgccggtgc cgcgccatcg tcaacagcga ggaatacgcg tccatctggc
    18961 ccgaccgggt gatgctgaag cccggcagct ccgcagtgaa cgagtgggag ctgaccaacg
    19021 attccgggct gctggccgcc ggcatcctgg gcgccgtcac ctccgcgcgc gccgacctgc
    19081 tgatcatcga tgacccggtg gccggccgcg aggaagccaa ctccgagacg atccggaaga
    19141 agacccgcga ggcctacgac gatgacctgc tgacccgcct gaagcccggc gccagcgtca
    19201 tcctgatcca gacgcgctgg caccccgagg acctggccgg ctccatcctg ccggaggact
    19261 acagcggcca gagcgggccg atcatgtgcc gcgatggcca ggtgtgggac gtgctgtgcc
    19321 tgcccgcgcg cgccgagcgt gccgacgacc cgctgggccg ggcagtgggc gagtacctgt
    19381 ggccagagtg gttcccgccg gagcattggc ggcagtacga gcagaagccg cgcacctgga
    19441 gcagcctgta ccagcagcgg ccgcgcccgg acgagggcaa ccagttcgag aacacctggt
    19501 tcgagtggta cgacgaggac gaggtgccca agcagctgcg catctacggc gccagcgatt
    19561 acgcggtcac caagaagtcg ctcactaacg accccgacta caccgaacac ggcatcttcg
    19621 gcgtggacca gaagggcgac atctggatcc gcgactggtg gtatgggcag gacgagtccg
    19681 acgtgaccat caccgccgag ctgcagctgg ccaagcgctg gcgcccggtg atgacctacg
    19741 gcgaatccgg ggtgatcgag aaggccatag gcccgctgcg cgcgcgcctg actcgtgagc
    19801 tgcaggtcag ggtgatgcgc gagtggctgc ccagcgtcag cgacaaggtt tccagggtgg
    19861 ccggcttccg tgccagggcc catgctcgga cggtccacct accgcgcggg aaggcctggg
    19921 ccaaccgcct ggtcgatcag ctggtggctt tcaacggcct gccgggcaac caggatgacg
    19981 gcgtggacgt gtgcggcctg atcggtcggg cgctcgatca catgcgggat gcgcacaaac
    20041 cgtccgttga acgccgggac aacctcgtgc cccatacccg cagatggctg gaatcccggc
    20101 ccgatgacga caacgacgtc gctgcccgta ggagaaacaa cctgtgacgc ccgagaccaa
    20161 cgatttcgcc ttcgtggacg cgctggacgc tgacgcgatg gccgagcagg agcgggcggc
    20221 cgaggccaac cggatcctgg aggaagaggc ggacgcaacc gcctggctca agcgcatcga
    20281 ggaggcccgg gagttcgaca aggccgcgcg caagggctac gccatcgacc gccggtactg
    20341 cgaggacaag gtcgatccgg aggtctacga cgtgtcggtg cctatcgccg gcacctacgt
    20401 gaacatcctg accggcttcc tctatgcccg cgaccccgag accagcgtgc agccggccga
    20461 gagcgccggt cccagccgca tcgaagacgc gaagatgctg tcgcgcacgc tcgagatcgt
    20521 gatcgccaac ctgtggaaga agggcaagct caaggcatcg gccgaccaga tggtgcgatc
    20581 gggcctgacc atcggcatcg gctggatcaa ggcggcatgg catcgcgaga ccgagcgcga
    20641 cccggccacc gagaagcaga tcaccaccgt gcgcgggcag atcgcccagc tgcaggccac
    20701 cgagcgacag ctggccgagg gcgacgcacc gaaccccgac gagctgcgcg cgcgctacga
    20761 gcagcagctg gccggcctgg aggcgagcgt cgagcaggtc atctacagcg gcctgtgcat
    20821 cgacttcgtg cgcggcgaag acatgcaggt ggccatctcc accccgtgcc tgaaggatta
    20881 caccggcagc ccgtggatcg cgcagcgcat cttccgcccg ctggacaagg cgaaggccga
    20941 ctatcccgag gtggctgatc gcctgagcag cgccaccctg ttctacaacg tgcgcccgtc
    21001 ggacgccgac aagcgctccg gcagcgtctc ggacgtggac gcggacgcct actccagcag
    21061 tggcagcccg cgcacggcca acgagagcag cggcgccaac ctctgcatct gggagctgtg
    21121 gaaccgggag accggccagg tcctgacgct ggccgtcggc ctcaagcgct acctgcgcgc
    21181 gcccttcacc cctgaccaga agtcgacccg gtactacccg ttcttccagt gggcgccgct
    21241 gtgggtcgat ggccgccggc acccgcagtc gctggtggac cgttcgcgct ccctgctgga
    21301 cgagtacaac cgcaccagga ccaattaccg cgagcatcgc cgccgcgcga tcccgaagat
    21361 ggggttcgat gccggtgcag tggaggcgag cgaggccgac aagatgaagg ctggcgccac
    21421 cggtgagatg gtgccgctga acctcaacgg cgcgtcaccg aacacggtgg tgttcccgat
    21481 ccagtacaac cagatcgacc cggcgctcta cgacacgtcg accattcgct ccgagctgga
    21541 gctgatctgg ggcatccagg aggcgctgtc gtccacgatc accgtggcca agaccgccac
    21601 cgaggccgac atccagcagc agggcaccga gtcgcgcatc gggtacgccc gcgacaccct
    21661 ggacgagatg ctcagcgagc tttcgaccta caccgccgag gtgtccatgt cgcccaacgg
    21721 gctgacgcac gaggacgtgg tggccatcgc cggtcccgag gccttctggg tcaacaccga
    21781 agagctgggc ctgatggact ccctggtggc cgtggacatc cgcgccggat cgtcgggcaa
    21841 gccggccacg gccatgaagc agcagcagtg gtccgtactt ctgccgcagc tgcagcaggc
    21901 ggtggtgcag atcggcgaga tgcgcggctc cagccggcag gacattgccg acagcctgga
    21961 acagctggtg gtggaaacca tcaagcgcac cggcgacacg ggcatcgacc cctactcgat
    22021 cattccgcag gcgccgccgc cgccgatgcc tgtcgtgccc ggcatgcccg gtgcacctgg
    22081 cgcccccggc cctgatggcc tgccgctccc cgcggcgaac gatcccgccg ctctggcgat
    22141 gcaagacccc ggcctgcagc tgccgcctga aatgctgccg ccccaactcc ccgccgccta
    22201 aacgaggacc accatggacc cgaacgccga aacccagcag gccgctgcgc ctgcgcccga
    22261 caccgaccag accgtgcccg atcaggtcga gaccccgtcc accgacaagc tggacgcctt
    22321 cgccgctggc gtggaggaag cccgcgccgc tgagacggcc gaggtcaccc cggctgctgc
    22381 gccgctggat gatgccgctg ccggcaatcc tgccgctgct ggtgctgcac cggtggaagg
    22441 cgagggtggt gaacctgctg cagccgctgc gccggccgcc ggtaccgatc ccgctgctgc
    22501 cgccccagac gccgctgcag ccgctgccca gcctgcgcac ggcaagaccg tggacgagga
    22561 gatcaaggac ctcggcatca cgaacgagcg cacgcagaag cgcttccgcg agctgtccga
    22621 acgcgccagc gaggccgact cgctccgcgg ccgtgccgag aaggtgagcg actgggagca
    22681 gaccatccag tccaccggca ccaacccgca gcagttcggc gccacgctgc tgtacctgac
    22741 cgacatcaac tcgggcgacc cggtgcgcat gaaccgcgcc tacgagacca tgcaggccga
    22801 gctgcagttc ctaggcgaga agctgggccg tgaagcgccg ggcttcgacc cgctgaccgc
    22861 gcatcccgac ctggccgaga aggtgaagac cggcgacctg gatcgcgcgg ctgccgtcga
    22921 gctggtccag caccgccagc gcggcgtgct gcagcaggag cagcaccaga accagcagcg
    22981 cgcatcccag tcggagcagg ccgcccagca ggggctgcag gcggtggccg agctgggtaa
    23041 ccagctgcga accagcgatc cgcagttcca gcagaagttc gcctacctcg cgccgacggt
    23101 cgagatcatc cagaccacca tgccgccggc gcagtgggcc gctgccatcc agcaggccta
    23161 ccagcgcctg ccgccgatcc ctgctgcagc accggcggtg gccgcgcgcc cgaacaaccc
    23221 agcgcgcgcc agcgccgcac cggcagtgcc ggccaccccg aagaacccgt ccgacgcctt
    23281 ctccttcggc gtggcggaag cacaggcgca gggccgatga gctggccggt tccgtcgaag
    23341 ctggccgggc agatccacgc catcctgatc gtccactacg gcatgtgggg ctgaggtggt
    23401 aacgtgtcgg cgtggtggat gcgcaggctg atgcgcgggc gtcaccccct taaacgatgc
    23461 caggaccaga aatggttggg gaactcggca gtcaattgtg ggtaaacctg ccatgccgga
    23521 gatcagcacc ggtcaccaca tcaagccccg gcacggccgg ggcttttttg tgtccgttgc
    23581 acatgggagg gaggctggga ttctggccgc tctttccgca acacccacca acggagccgc
    23641 cagtaatgaa cgacaagacc atcgagcagg aaatccagtc caagggcctt accgcaccgc
    23701 gcgtcacgcc cgctgacatc gaagccgaga ttgcaggcga acacttcttc actgctggcg
    23761 acggctatgc cggcagggcc gcgttgacgg tcgaagaggg tggcgtcatt gaaacgccgg
    23821 agtcgctcga cctgctgact ttctgcgtgc tggtgctgcg caacggcttc accgtcaccg
    23881 gcgagtcggc ctgcgccagc ccggagaact tcgacgccga gatcggccgc aagatcgccc
    23941 gtcagaacgc cgtccagaag atttggccgc tgctgggctt ccggttgcgc gaccagctgg
    24001 cgaaggaggt ccatggttcc ctacaacgcc tacttcgcca tcaagaacgt caacgacacc
    24061 gtcagcacct gggtgccgag cgttaacgac tgcctcgctg acgactggta tgtgctgtcc
    24121 gatgtggtgc tgaccgacgc cgatgccgag gctgacctcg ccggcacgcc acggcccgac
    24181 catccgaccg cagcctgacc ccaacgaccc cgctccggcg gggtttttca tgtccgttga
    24241 cagtcccgac ccctcgcgca tagtcgcccc catcggcaga tgccgacacc gcgtgtgacg
    24301 taagccgggt tcgccaccgg tagcgctgta ctgagggatt cgcgctcctc gaacgcggaa
    24361 agacccaagg cccaacgggc ctccttcttt cccttcgagg ctacaaccat gcccttgacc
    24421 actgcccaga tgctggccgg tgccaaccgt cagatggagt cgtacgcaac caacgacccc
    24481 atcgaccagt tctccaccga gcgcccgttc gcctcctggc tgatcgccaa caagaaggaa
    24541 tcgaccttcg gcaacggcat cttcaacgag aaggtgcgga tcggtaacga ctccaactac
    24601 cagaactaca ccggtgacga ccaggtcacc tacaaccgga aggacaccgt ccggaaggcg
    24661 ccgttccagc actacgaagc gcacgacggc ttcacgctga acgagaccga gctggccaac
    24721 aacggcatca tcctgaccga cgacaagaac gcggtcatga gcgatgccga gaagatccag
    24781 atcgtcaacc tgctggatga aaactggtcg gcgctgaagg atggcttcca ggaaaactgg
    24841 gacatcgagg tccaccttga cggctcgacc aacccgaagg ccgttccggg cctggacgcg
    24901 ctggtcagca ccaccccgaa cgtgggcgtg atcggcggca tcgatgcggc cacctcgccg
    24961 tactggcgca acttcgccga catgggcatt gccacgggca ccgccggcaa cctgatcagc
    25021 cacatggaaa ccctgtggcg ccagaccatc acctacggaa agatgggcca gcccaacgcc
    25081 atcttcgtgg gtgcgttgat gtacgacgcc atccaggctg acgccctgaa ggtgatgtcg
    25141 cgccagatca ccatcggtgc caacgccacc ggcggtatca ccctggaccc gtccaccaaa
    25201 gcgctgtcgt tcaagggcgt gccggtcgtg tgggatccgt ccttcgaggt catcgacgcc
    25261 cgcttgggtg cgatcaccta cccgtggacc aagcgcggct acttcctcaa cagcaagacc
    25321 ctgaccctgc gtccggtcca gggccgctgg atggtgaagc gcaccccgcc gcgggtgtac
    25381 gaccgctaca cgcactactt cgggcagacg gccgattacg gcctgaccac gaagaagcgc
    25441 aacagcaacg cggtcttctc gatcgcctga cccctatcag ccggcggggc agcccctcgc
    25501 cggcgggaga cctgaaatgc ccaacatcaa gaagatcgcg gtcccggcca ctgtcagcgc
    25561 aggcgctgcc gttgccctgg acgtcacccc gctgctgggc ggcgaaggcc gtgagggcct
    25621 gctgtatggc ccggccggcg gcctcggcac cggtgtggtg cagctgcagg gcgcgccgaa
    25681 gacggccgcc ggcgtggctg gcacctacgc cacgctgatg accctcaact cggcgaccac
    25741 cttcccggtg gaaatccccg acctgcccaa cttcatccgc gccaacgtaa ccaccgcgcc
    25801 ggcggcggcc accgagctga cgctcgaagg agtccagtaa tggccaagac caagatcccg
    25861 cacgtgctgc tgctgatcga tcgcgacgcc agcacgaaaa tcccggtcat ggtgccggag
    25921 tacgaacaga ccatcctgga agagatctac ggggaagatc tggtccacga agtcgagggc
    25981 caggccaagg acatcgaggt cgaggacttc gacgtgcaga aggccttcga cggcctgatg
    26041 tcgaagtacc aggccacggc cgagggcgac catgcccgca agctgatcta cccgaagctg
    26101 cgtgacctgg aaaagcgcct gaaggccttg ggcgtgaagg tggccagcaa ggatgacgac
    26161 ggcggtgacg acagcctggc cgccggcacc atcgcgcaga tcaccgcggc gctggctgac
    26221 ctgtccgacg aagagctgga tcagctggcg gaagacgaag cggccggcaa ggaccgcgcc
    26281 ggcgtccacg ctgcgatcga agccgagcgc gagaagcgca ccggcaacca gtaatccccc
    26341 gctggcggcg agggtggcgg ccggccgggg tgacctggtc ggccgtcttt atatcgggaa
    26401 ccacaatggc agaccccatc agctacaact gcgagtgcga cgacgactat ccccggacca
    26461 cgctgctgga gatgcgcaag cgtctgctgc gtcgtctggg gttcgccgcg caggccaaca
    26521 acccgccgcc gggcatggcc gacctgctca acgacttcat caccagcgcg caggagctgc
    26581 tgttccggcg ctactcggtg atgcggcgcg agcgcttcta cacctgggat ctggtggccg
    26641 gcacgcgctt ctacgacctc gacgccaacg ccgacgagtg cacaaagcag ctggacccgc
    26701 gcatgatcac ctgggccgga ctgtcgcagg gagacggcaa ctggcgcccc ctgtacagcg
    26761 gcatcgaccc ggtgatgtac acggcgcgcg ggcccggcat tcccagccac tacgaggtcc
    26821 ggcagtgcat cgagctgtgg ccggcaccgg cggacgctac ctggaagctg cgcatcaagg
    26881 ggcagttcgg cctgatgccg ctggtagccg acagcgactt caccaccgtc gacccggagg
    26941 cgctgttcct gctggccctg gccaacgcca aggcccacta cggccagcct gatgccggca
    27001 actacgcatc gcagctgtcg acctacgtgc gcgacctgat ccgcggcgac cacctgacgc
    27061 gccgctacat ccctggcacg tcggacccgc gaaacgctgt ccgtccggta ccggtcggcg
    27121 gctggccgga ggacacgcca tgaggcagca gtctctctcc acggtaaagg ccggaatcac
    27181 ccggctgcgc gacaagggtg gcgcgtcgca ggactcggtg tatgacctgg tcaacggcta
    27241 tgtcacggcg gcgcggacca tcaagtcgcg gcctgggacc aggattgctc acgtgctgcc
    27301 ggccggcacg aaaggactgg tgtttttcca gggcaagttc gtcgtgttcg caaatcaggt
    27361 gacggcatcg ccctcgccac aggtcgagat tgaggttttg cggcacccga ccgtgcccgg
    27421 cgccccgatc aaggaaatcc acttctccat gcccttcctc ggctacctct acgtggtagc
    27481 ggagttcgaa ggtgcctctg acccgactta cgtccacttc tggctggaga agggcgaggt
    27541 ctggcagccg aacaaaacct acttcccggg cgcgctggtc aggccgacca cagggaacgg
    27601 cctggcctat cggctggagg gcgatatctc caagtacctg ccatgggctc cgaacgtcgc
    27661 gcgcgccgtc ggcgatgttg tggtgcctac catcgacaac ggcttcaagt acaccgtcac
    27721 cgacaccact ggcgcgtctg cccgctccgg cacgaccgaa ccggcgtggc caaccaacgc
    27781 cggcgagaca gtctatgagg acgccaacat ccccgacgca cttggctcga acaccaccac
    27841 cacaccagcc gtaccgccca acgtgaagga ccgctacggt ccggggagga gctgatggcc
    27901 gtcccaacct ggcagcctgg caccctctac cagcccggcg acatcgtcca gccgatcacc
    27961 gctccgccgc cgacggccgc gcaggtcgag aacggcgact tcgcagctgg caacgtgaac
    28021 tgggatttca tcggtggcgc ggcgttcgcc accagcggcg gatattccgg caacggcaac
    28081 tgcgtccgca tgcccggaaa cgtcgcggac ggcattgcgc tcaaccgcac aaagctggtg
    28141 gtccccagca ccggcagcac cttcgaagcc agcgcgatga tcaaccaggg cgcgtcgatc
    28201 cggggcgcca cgcgcggctg ggtcgaggtc cggtggttcg acatcgatga tgcacagatc
    28261 ggggctgagc gcggcaacgt cgtagacgac ggcagcggcg gcgcatggca ccagtccaag
    28321 gtaactgcca cgcggccggc cggagccgcc tacgcgcgcg ccggcatcgg cctgttctct
    28381 gtagctgatc acagccaccc catctggggc gacaacctcg cggtgtcggg cacgttcgcg
    28441 ggtcttcccg acgggctggt ttacaaggct gtgcagccgg aatccggttt ctcggacagc
    28501 gatgagcctg catggccgcc gctacttggc cagcaggtca tcgacaacga cgtgatctgg
    28561 gaggcagtcg cctccacgcg cgtcacctgg gaggcgtcgc cgctatatgt gagtggcgcg
    28621 gtcgagccgg tatggcccac ggacattggt ggtttcgttc gagacggaac gatcgattgg
    28681 aagacggtgt ctcgccgggt tgaggacccc aactgcccgg actcaaagac tgtggtgatc
    28741 gcggcgagca aggtttttgc cggtgacgac gacatcgtgc gttacagcgc caccgtcaac
    28801 cctctggact ggaccacagc ggacgacgct gggtacctgc cgaccgggct gcagaactac
    28861 ggatctaacc cagtagcagc catgggcctt taccgcggaa atttggtcgt gttcaacgct
    28921 gagggattcc agctgtggca ggttgatgaa gaccccgcaa gcatgtctct gattgacgca
    28981 ctcccgctgg gcagcaccca gcatcggtcg atcgcgccag tgtccaatga tctgctgttc
    29041 ctcgcttccc aaggcgttcg gtccatcgga atcgcagcca gctccaccaa ctaccagact
    29101 ggtgatgttg gcatgccaat cgacccgctt gtcgattcgc tcatgaagga ggtagggccc
    29161 caggggacga gcccaaaggg gctgtatcac cccgcagcag gccagtacat gttgattttc
    29221 aggggcacgg gtaatgcccg tccttccaat ctgcttctgc aggagaacgg tgatccgatc
    29281 ctccaggagt cgttcgacta catccacttg gaggcataac gatggccgat tccccgattt
    29341 ctatgcttcc tcaatccggc gcactgacgg gcgctgaact gctggttttg gtccagggag
    29401 acgtgacctg caaagcaacg cttatgcagg cgcttgcatt gctatcaagc cggtgcgtgt
    29461 gggacaggaa tatctacaac agctcgatgg gtggcgtgat cagcaattct gggctgaccc
    29521 atacggcgac attggctggg atcgggaacc tggttgttgc cagccgcgcc cattctgctg
    29581 gaaagcgcta tttcgaagtc cggatggatg tgattgccgg agcgggaacg ccaacggttg
    29641 gagtttgcca atcaaactcg agttctgccc aagtaggcca agggcccgcg ccatccacga
    29701 accgaaactc tgattgggga ttgctcgcaa gctccgggaa caagtacgca gccggaacct
    29761 ctactgcgta cgggtctgcg cttgctgcca atgacattct catggttgcg ctggacttgg
    29821 atgcagacct gatctggtgg ggcaagaacg gggcttggtt tgcggccggg aaccctgccg
    29881 caggcactgg agctgcgttc aacaacgtgc aggacttcgt gtatcccgcc atcacgaact
    29941 cagcaggctc caaggtcacc gcgaactttg ctggcccgtt ccagttcgcc ccgcccaccg
    30001 gcttttctgc gtgggatagc taaatgaagt cctcgaccat cctggtgtac tcaatgagct
    30061 caaccggtca ggttggcgcg tggtcgctct acaccatcgc gttgaacgtt gatgagtggt
    30121 gcatcgcagg agacgaccta tacctgcgat ctggcgacta catccacgta tttgacgaag
    30181 aagaagtcgg cgacgagacc ggcccaggca ttgttggcga gtttcagggc ctcatccagt
    30241 ggccatggct cgatttcggc cagcctgggg tcacaaagag cctttacggc ttcgatatgg
    30301 tcggaatcgg cgacgttgcg gtcgaatttg gctacgacca gtccaacggc ggcgctttca
    30361 ccgagggcta caccgtccca gccgacaccg tgccggggat ggtgatcccg atgcccattt
    30421 cggcgccttc cttctcggtc cgactgcgct acagcggcac cgagcagtgg caatggaacg
    30481 cgttttccct gtacctgcag gatctgcggg ggatggcgtg attccgttga acgacatggc
    30541 cgagcgtgaa gaatctgcac atgcagaccg cccgattgcc ctccaacgtc atcccgtgcc
    30601 ggccggtgca cctggtcgcg ctggccgagt gcatgcgcga gaccgagcag gcgcagctgc
    30661 tggccgtcat gggcacgccg gcctacagcc cggacaccgc ggcgcactgg ctgatcaaca
    30721 cctgggccca gtcggcgcct ttcgcgctca cggtcgtcgg cagggatggc atgcccgccg
    30781 ctgctggcgg gttccaccca gtttccccgg gcgtctggca gtcctggatg gttggatcgg
    30841 agcagggctg ggccgagcag tggcgcgcga tgaccaaggc cacccgctgg ctgatcgacc
    30901 ggctgctgga gacgcaggct caccgcgtgc agaccagcgc caccaccaac agagagaagg
    30961 ccatcgagtg gttcgaacgc tcgctgggca tgcggcccga gggcgtctcg cgcggctacg
    31021 gactcaaggg cgaggacatc gcccactttt caagaatgcg gggtgaatga tgggcggcgg
    31081 aagcaatggc tcggccacga aggcggcgca ggaggaatcc tggcgccaga acaacatcga
    31141 ccaggccgtc aaccagatca accagatcta tggcggcgca tctcgacagg cggacatcaa
    31201 cgacttcctc ggggcgtcgc ggtcgttcta cacgaacgag ctggagaagc agaagggcgt
    31261 cgccgaccgc agcctgaagt tcgccatggc gcgcaacggc ctgaccggcg gcaccgcctc
    31321 gatcgacgcg aaccggacgc tgggcgagaa ctaccagtcc ggcatcctca gcgccgaccg
    31381 cttggcgcag caggcagctg ctgacctgcg caccgccgac gacaactcgc gcatgaacct
    31441 gatctcccag gccagcaccg gcatgggcct caccagcggc gcgcagcagg ccgcacaggc
    31501 catgcaggcc aacctgcagg gcagccgtgg ggccatgaag gctgacgccc tgggcgacgt
    31561 gttcggcggc ctggccaacg tctacaccac cagcaaaaac atggctgagg aacgccgcgg
    31621 caaccgcgcc tatggcctgc tgtatcagcc cggcttcggc gctggcgcgg cgggtggccg
    31681 atgagtctgg ccacgcccat tgccgtgctg gacgcgatga tggcggcggt cagccatccc
    31741 gagctggccg atatctgcca gctgcaggcc gccatgcagg cgcagccaca ggccgacctg
    31801 ccggtggagc acaacttcct gcccgggcag tacgtgcgca cgctgcggat gccggccggc
    31861 tcgctggtgg tgggcaaacg ccaccgccac cgccacctgc tcatcgtcac cgggcacgtc
    31921 accgtgcgca ccgaggccgg catggtcgag ttgcagggca cgcacatcat cgactcgctg
    31981 cccggcatga agcgggccat ctacgcccac gcagacagcg tgctgatcac cacgcacctg
    32041 accgacgaaa ccgacctcga caagatcgag gcctatgtga tcatgccgga cgacgcggct
    32101 ctggaaatcg aaggggaaac gaaatgacct gggtcgcaac agccattgcc atcattggcg
    32161 ccggcgtctc gtaccacaac ggggagcaga ccaagaagcg gcaggacaac gagctcggcc
    32221 ggcagatcca gcagcgctcc ctgcggcagc aggaagccga caaggaggtc atggacaccc
    32281 tcgccgagcg cgcggggaac aacggcgaag ctgcgcggtc ctcgatgctg gaccagtaca
    32341 tgaaccaggt gcgtgcagcg cagggctcgg ccacgtcggg gctgcggcag gtgggcggcg
    32401 tctccgatgc ctaccgccag tcggccaacg atgcagcgct cggcatcagc gactacgcgg
    32461 gcaacaccgc cagcctgatg agccgcatcg acgcgccgat gcgccagcgg cagggggaag
    32521 cgcagcagaa cgcgcagctc agcaccgcgt tgaacctgat cggcaaccgc tctggcagcg
    32581 acgacttcct ctcgcagctg cgcctgcagg gcatccgccg caacgccggg ctggacgcgt
    32641 tctcccagat cgccggggcc tacgcaggcg cagccggcgg cggcggtggt ggtgcatcga
    32701 gctccaccgg cctgtcgaac caggccaacg gcatcacggc ggccaacaac gcgcagatct
    32761 tcaacaattc gcagaaggcg tggggctact gacatggcag acctgatgag cgtgctgcag
    32821 gcaaacagcc agcccccgca gggctgggca tcggttggcc aggcgctggc gggcttgggc
    32881 ggggcggggc gcgaggacgc ctacaaccgc ggcatggcca aggccgcgca gttggaccag
    32941 ctggtgcaga ccgcgcgctt cagccgcgag aaggcgatgg aggccgagga cgcgcgcaag
    33001 ggccgcggag gtttcgcgcc ggccgcgatc gctgctggca tttccgaggg tcaggctggc
    33061 ctgctctcga acgcgctcat ggccggttac gaccccacga aggtcagtgg ctatctgggt
    33121 gcgacccagg agcaggggtt ccgtggcgat gcggtcgcgc gcgcactggc cggcgacttc
    33181 ggtgctgcca acgcgaacct gatgggcgtg gccaacggcc cggtcgagct cgcctcggtg
    33241 cagggccaga acctcatcaa caaccggctg ctggagggtg gcggcggcat caccaccacc
    33301 gaacagggcc gagccggcat cgctgctgat gccgcgcgcg cagcgtccag ctacgccagc
    33361 gccaacagct cgaacgcatc cgccgcgcgc acccggcagg ctatgtcgct cgatcgtgcc
    33421 gacacgatgg gtggcggcgg cgcccgcggc gccatgaagg ccccgagtgg ctaccgctgg
    33481 accagcgatg gccggctcga ggcgattccc ggcggccctg cggacaatgg caatgccggc
    33541 ggcgcgaagg cgaccggcga cgagctgaag caggcgtttg ccggcaagca gatggctgag
    33601 gatgcctacg acttcgccgc agccttcttg gggaagacac ccgaggcggt ccggcagatg
    33661 agcgcggacg agatccggaa gcagatcaag cagggcggcc gcctcaccgc ggggccggtc
    33721 attggccgca tcccgggcat gagcgcgctg gccaacgccg acctggacgc ttacaccagc
    33781 tccgctgcag ggcgccaggc tcgcatcaac aaccccaccg gcccggtgac ggacgccgat
    33841 ttcaaggcag cagagcggtc ggtcttcagc gcggccaaac cggcgggcgt gaatgcagac
    33901 ctggtctacc gagcgctgat gcgcggcgcc ggccagcagc agaacgccgc cgggcagtcc
    33961 cgaccggctg aggtccggcc caccaccggc caagccccgg ccatcggcag cacgcgcggt
    34021 ggatatcggt acgtcggcgg caacccttcg gatcgcagca gctgggagga actctgatgg
    34081 cactgccctg ggaggaatac cagcaggtgc aggccggcgc cgcgcgcgcg ccggcagc
//
LOCUS       DATCUN010000249         6049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_180886, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000249 DATCUN010000000
VERSION     DATCUN010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180886"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1953)
                     /locus_tag="VIO83_09655"
     CDS             complement(<1..1953)
                     /locus_tag="VIO83_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8331577.1"
                     /translation="MPWINNLKLMPKLMLTFGVLLLVMLLQGIVAYRGLYSLNNVTTE
                     LAGHRMESIRTAGEIRGMVGEYRNSAYQGLIRASDDVKADAKKRAADLRGKIDLSIKK
                     YPELIDNPQQKKLFDTFAKDWKDSLASYDAVTEMLELDLPDDAVDTFVGETRTKHQKA
                     SSALEALIAEDNRLARASREEAESTYAASATLTIIALLGGAALGLVLVYLFARSLVGS
                     MRGAVSVANDVAGGKLDGHIDVSRKDEVGDLMQAMQRMQRDLRERTETDQAIARENLR
                     IRTALDYSSTGVYLTDNQLNIVYANRALEQSLSQYQEELRKHLPDFDANTSLVGRPLT
                     VLEHRGEMDATLLNNLKANGVARRAMQFGDAQFAQVVSTIRNEEGESVGHVVEWRDRT
                     PEALVEAEVARVIAQAAAGDLSGRIRAEGKEGFFLQLAQQINGLLDANAASIEQISGL
                     LTALSQGDLTVRMHGDFQGVFATMRDDANATAEQLSGIVTKIKHSSQAISSAASEIAT
                     GNSDLSRRTEQQAANLEETAASMEELTSTVRQNAEHARQANQLAIGAHSVASQGGDVV
                     GQVVTTMSAIQTSSKKIAEIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASE
                     VRTLAQRSAGAAKEIKGLIDDSVGKVN"
     gene            complement(2050..2433)
                     /locus_tag="VIO83_09660"
     CDS             complement(2050..2433)
                     /locus_tag="VIO83_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331578.1"
                     /translation="MKSLLALFSVAVLASSASSAFAQSAEMIPPEMAPRSVGKDGMVC
                     GKVEKARYAEGSEGQPTFLYMGGAFPRHTFSARIAGANRGKFSFPLETLEGKTVCVIG
                     KIARDASRAEIEVSSPAGLKLANIK"
     gene            complement(2585..3076)
                     /locus_tag="VIO83_09665"
     CDS             complement(2585..3076)
                     /locus_tag="VIO83_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015474932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY8331579.1"
                     /translation="MNDSNPNAASSGGEYLSFTLGAEHYGVDILKVQEIRGYDSVTRV
                     PDAPDYIKGVINLRGTIVPVIDLRLKLRLEKATYDAFTVMIVLNVEDRVVGIVVDSVS
                     DVIPLNADQIRPTPEFGAAVDTRFISGIGTQDDRMLILLDIETLLDSADMGQPAQVED
                     AAA"
     gene            complement(3621..4400)
                     /locus_tag="VIO83_09670"
     CDS             complement(3621..4400)
                     /locus_tag="VIO83_09670"
                     /inference="COORDINATES: protein motif:HMM:NF018967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HEY8331580.1"
                     /translation="MSEGSFEAHDDDHAAHDDGADDRYLVRNPRQIRQLLQALIDQRS
                     LINAHLGGRDQSFPTAILELDEDEDMLLLDGSPSEPANRAAEEAGHLLCFAQLDKVMV
                     RFRLDHLERVGSTHHVAFRVAFPSELVHLQRRELYRLETPITDSPQLLFPINEDRSEA
                     LSLRVVDISGGGLAVTVPVDCNVFALQKRYDARLDMPEGGPLQIALIVCNQLAVKLPN
                     GVEMKRVGMRFDSLPRGGDSAIQRYIFRIDRQRSARKNGES"
     gene            complement(4577..>6049)
                     /locus_tag="VIO83_09675"
     CDS             complement(4577..>6049)
                     /locus_tag="VIO83_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8331581.1"
                     /translation="MTSMLRRYSEETRQMAAMHAGPDISHRMPTDFPGVYGELAVGTN
                     TVVFDHLDAIKDAIAVLNQYAAGDLSPNARRLPGSRAILHESMDAAKASLLAINTQIQ
                     SLAQAAAAGDFTQRGDALRFDHDFRVMIEHLNTMMEVADGNLSQLSHLLQSIANGDLT
                     ARMQGQFHGVFASMRDDANTTVAQLTQIVGRIQHASSSISTAAGEIASGNNDLSRRTE
                     QQAANLEETAASMEELTSTVRQNAEHARQANQLAIGAHGVASQGGDVVGRVVSTMHEI
                     DASSRRIADIITVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSAS
                     AAKEIKGLIDESVGKVAEGSALVNQAGETMGEIVASVQRVTDIMAEISAASQEQSAGI
                     EQVNQTITQMDETTQQNAALVEEATAAARSMEEQAGHLTDAVALFKLDGQAAPMASAP
                     AASSRASAVAAPRARAAAAPVRRAAVREPVLANEGDWQEF"
BASE COUNT          933 a         1908 c         2095 g         1113 t
ORIGIN      
        1 gttgaccttg ccgaccgagt cgtcgatgag gcccttgatt tccttggccg caccggccga
       61 acgctgggcc agggtgcgca cttcggaggc gaccacggcg aagccgcggc cctgttcgcc
      121 ggcacgcgcc gcttccacgg ccgcgttcag cgccaggatg ttggtctgga aggcgatgcc
      181 gtcgatgacc gagatgatct cggcgatctt cttggacgag gtctggatcg cgctcatggt
      241 ggtcaccacc tggccgacca catcaccgcc ctggctggcc acgctgtgtg cgccgatggc
      301 cagctggttg gcctggcggg catgctcggc gttctgacgc acggtggagg tcagttcctc
      361 catcgaggcc gcggtttctt ccaggttggc cgcctgctgc tcggtgcggc gcgacaggtc
      421 gctgttgccc gtggcgatct cgctggccgc cgagctgatg gcctggctgg agtgcttgat
      481 cttggtgacg atgccgctga gctgttcggc ggtggcgttg gcgtcgtcgc gcatggtggc
      541 gaacacgccc tggaaatcac cgtgcatgcg caccgtgaga tcaccctgcg acagcgcggt
      601 gagcaggccg gagatctgct cgatgctggc cgcgttggcg tccagcaggc cgttgatctg
      661 ctgggccagc tgcaggaaga agccttcctt gccctcggcg cggatgcggc cggacaggtc
      721 gccggcggcg gcctgggcaa tcacgcgcgc cacttcggct tcgaccagcg cttccggcgt
      781 acggtcgcgc cattcaacca cgtggccgac actctcgcct tcttcgttgc ggatggtgga
      841 gaccacctgg gcaaactgcg cgtcgccgaa ctgcatggcg cgacgggcta cgccgttggc
      901 cttgaggttg ttcagcaggg tggcatccat ctcgccgcgg tgctcgagca cggtcagcgg
      961 acggcccacc agcgaggtgt tggcatcgaa atcgggcagg tgcttgcgca gctcctcctg
     1021 gtactgcgac aggctctgct cgagtgcgcg gttggcgtac acgatgttga gctggttgtc
     1081 ggtcaggtac acgccggtgg aactgtagtc cagcgcggtg cggatgcgca ggttctcgcg
     1141 ggcgatggcc tggtcggttt cggtgcgctc gcgcaggtcg cgctgcatgc gctgcatggc
     1201 ctgcatcagg tcgccgactt catccttgcg gctgacgtcg atgtggccgt ccagcttgcc
     1261 gccggccacg tcgttggcga cagacacggc gccgcgcatg ctgccaacca gcgaacgggc
     1321 gaacaggtac accagcacca gaccgagtgc ggcaccgccg agcagggcga tgatggtcag
     1381 ggtggccgag gcggcatagg tcgattcggc ttcctcgcgc gaggcgcggg ccaggcggtt
     1441 gtcttcggcg atcagtgctt cgagcgcgga cgaggccttc tggtgcttgg tacgggtttc
     1501 gccgacgaag gtgtccacgg cgtcatcggg gagatccagt tccagcatct cggtgacggc
     1561 atcgtaggag gccagcgagt ctttccaatc cttggcgaag gtgtcgaaca gcttcttctg
     1621 ctggggattg tcgatcaatt cggggtattt cttgatggac aggtcgatct tgccgcgcag
     1681 gtcggcggcg cgcttcttgg cgtcggcctt gacgtcgtcg ctggcgcgga tcaggccctg
     1741 gtaggccgag ttgcggtatt cgccgaccat gccgcggatt tcaccggcgg tgcggatgct
     1801 ctccatccgg tggccggcca gttcggtggt gacgttgttg agcgagtaaa gcccgcgata
     1861 cgccacgatg ccctgcagca gcatcaccag caggaggacg ccgaaagtca gcatcaactt
     1921 cggcatcagt ttgaggttgt tgatccaggg catgggcggt gcgatctcca gtggcaaacg
     1981 cgtccgggcg tcgtgcccgg ttgcagcgac cacgccggcc agcgccggcg tggcatggcg
     2041 tacggcggat tacttgatgt tggcgagctt gaggccggcc ggcgagctga cttcgatttc
     2101 agcacgcgat gcgtcacggg cgatcttgcc gatcacgcag acggtcttgc cttccagggt
     2161 ttccagcggg aagctgaact tgccgcggtt ggcgccggcg atgcgggccg agaaggtgtg
     2221 gcgcgggaac gcgccgccca tgtacaggaa ggtcggctgg ccttcggagc cttcggcgta
     2281 gcgggccttt tcaaccttgc cacagaccat gccgtccttg ccgaccgagc gcggcgccat
     2341 ttccggcggg atcatctcgg ccgactgggc gaaggcggac gaggcagagg aggcgaggac
     2401 ggcaaccgag aacagcgcaa gcagggactt catcgggaag aatctccggg ggaaattgga
     2461 cgagttggcc cggccttggg cagccctaca cagccgggtc ctgaagcagc tatcggccgt
     2521 cgcgcaggga actgaagaac ttttttcaca tcccggtcga atcgaccggg atggcggggc
     2581 acgatcaggc ggcggcgtct tcgacctggg ccggctggcc catgtcggcg ctgtcgagca
     2641 gggtttcgat atccagcagg atcagcatgc gatcgtcctg ggtgccgatg ccggagatga
     2701 agcgggtatc gacggcggcg ccgaactcgg gggtggggcg gatctggtcg gcgttgagcg
     2761 ggatgacgtc ggagacgctg tcgacgacga tgccgacgac gcggtcttcg acgttgagca
     2821 cgatcatgac ggtgaaggcg tcatacgttg ccttttccag gcgcagcttg aggcgcaggt
     2881 cgatgaccgg gacgatggtg ccgcggaggt tgatgacacc cttgatgtag tcgggggcat
     2941 cgggcacgcg ggtgaccgag tcgtagccgc ggatttcctg gaccttgagg atgtccacgc
     3001 cgtagtgctc ggcgccgagg gtgaagctga gatactcgcc accgctgctg gcggcgttgg
     3061 ggttgctgtc gttcatggaa ggaatcctgg gtcgggcgtg gcggtaagga aaaaccgggc
     3121 gtcacgctca atatcggcct ggagggcagc gactttagag gaggtggtct gttgcggcgg
     3181 acagctattc actgctgatg cgcggcgggc ggtgccaggc cctgccggga cacgccgcaa
     3241 gtacgtccgt gtaggctcgt gagcgccatc catggcgccc aacggtcccg gcagggcctg
     3301 gcaccgcccg ccccacagat tccgtgatcg gttggataaa gccgcgcggt ggtgggggag
     3361 ggtagtgccg agcgttgctc ggcacctaaa cccacccctc aggcgctgcg tgctcttgat
     3421 ctcaacgcgg cgaaagccat ctgcgctggg gcggtacggg tgggttggcg ggaccgttgg
     3481 gcgccatgga tggcgcccac gagcctccaa ggatggattc acggcgtgtc ccgccaaccc
     3541 acccgtaccg acccacccca gcagcaccga accgccgcag ttgcagttgc agttgaagtc
     3601 gctccaaaaa agcagccccc tcaactctcc ccattcttgc gggcgctgcg ctggcggtcg
     3661 atgcggaaga tgtagcgctg gatcgcactg tcgcccccgc gtggcagcga gtcgaaacgc
     3721 atccccacac gcttcatctc caccccgttc ggcagcttca ccgccaactg attgcacacg
     3781 atcaacgcga tctgcagcgg cccgccctcc ggcatgtcca gccgtgcgtc ataccgcttc
     3841 tgcagcgcga acacattgca gtccaccggc accgtcaccg ccagcccccc gccactgatg
     3901 tccaccaccc gcagactcaa cgcctcgctg cggtcctcgt tgatcgggaa cagcaactgc
     3961 ggagaatcgg tgatcggcgt ctccagacgg tacaactccc gacgctgcag atgcaccagc
     4021 tcgctcggaa acgctacccg gaacgccaca tggtgcgtac tccccacgcg ctccagatgg
     4081 tccagccgga aacggaccat caccttgtcc agctgcgcga agcacaacag gtgcccggcc
     4141 tcttccgccg cccggttcgc cggctcggac ggactgccgt ccaggagcag catgtcctcg
     4201 tcttcgtcca gctccaggat cgccgtcggg aacgactggt cccgcccgcc caggtgcgcg
     4261 ttgatcaacg agcgctgatc gatcaatgcc tgcagcagtt gacggatctg ccggggattg
     4321 cggaccaggt aacgatcgtc cgcgccgtcg tcgtgcgcgg cgtggtcgtc gtcgtgtgcc
     4381 tcaaagctgc cttcggacat ggatgcgtgc tgaatagagt gggcgccgcc ggcggtttta
     4441 ccagggctct gtccgtttat cggcagcggt tcacattaat gtagggccaa tccgtgtaga
     4501 tcagctcggc cgccccaaac gcacgacacc ccgccgaagc ggggtgccgt ggacacgcat
     4561 cacagggcag ggcggatcag aactcctgcc agtccccttc attggccaat accggttcgc
     4621 gcaccgcagc acgccgcacc ggcgctgccg ctgcacgcgc acgcggtgca gcaacagccg
     4681 aggcacggct gctggccgcc ggcgcgctcg ccatcggggc cgcctggccg tccagcttga
     4741 acagggccac ggcgtcggtc aggtggccgg cctgctcttc catcgaacgg gcggcggcgg
     4801 tggcttcttc caccagcgcg gcgttctgct gggtcgtttc gtccatctgg gtgatggtct
     4861 ggttgacctg ctcgataccc gccgactgct cctgcgaggc ggcggaaatc tcggccatga
     4921 tgtcggtcac gcgctgcacc gacgccacga tctcacccat ggtctcgccg gcctggttga
     4981 ccagcgccga gccttcggca accttgccca ccgactcgtc gatcaggccc ttgatttcct
     5041 tggccgcact ggccgagcgc tgggccaggg tgcgcacttc ggaggccacc acggcgaagc
     5101 cgcggccctg ttcgcccgca cgcgccgctt ccacggccgc attcagcgcc aggatgttgg
     5161 tctggaaggc aatgccgtcg atcacggtga tgatgtcggc gatgcggcgc gaggaggcgt
     5221 cgatctcgtg catggtgctg accacgcggc cgaccacgtc gccgccctgg ctggccacgc
     5281 cgtgcgcgcc gatcgccagc tggttggcct ggcgggcgtg ttcggcgttc tggcgcacgg
     5341 tggaggtcag ttcctccatc gaggcggcgg tttcttccag gttggcggcc tgctgctcgg
     5401 tacggcgcga caggtcgttg ttgcccgagg cgatttcgcc ggcggcggtg ctgatgctgg
     5461 acgacgcgtg ctggatgcgg cccacgatct gggtcagctg ggcgacggtg gtgttggcgt
     5521 cgtcacgcat gctggcgaac acgccgtgga actggccctg catgcgtgcg gtcaggtcgc
     5581 cgttggcgat cgactgcagc aggtgggaca gctgcgacag gttgccgtcg gccacttcca
     5641 tcatcgtgtt gaggtgttcg atcatcaccc ggaaatcgtg atcgaagcgc agcgcatcgc
     5701 cacgctgggt gaaatcaccg gctgcggcgg cttgggccag cgactggatc tgggtgttga
     5761 tggccagcag gctggccttg gcggcgtcca tcgattcgtg caggatcgcg cggctgccgg
     5821 gcaggcggcg ggcattgggc gacaggtcac cggcggcgta ctggttcagc acggcgatgg
     5881 cgtccttgat cgcatccagg tggtcgaaca ccaccgtgtt ggtgcccacg gccagctcac
     5941 cgtacacgcc cgggaagtcg gtgggcatgc ggtgcgagat atccgggccg gcatgcatcg
     6001 cggccatctg ccgggtttcc tcggaatagc ggcgcagcat gctggtcat
//
LOCUS       DATCUN010000250         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_67334, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000250 DATCUN010000000
VERSION     DATCUN010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67334"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1746
                     /locus_tag="VIO83_09680"
     CDS             <1..1746
                     /locus_tag="VIO83_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HEY8331582.1"
                     /translation="LAVVVAIAMSVWRSRQARKVTTYGSARWADVADIRKAGLTQPAG
                     VFLGQHDGHYLRHEGPEHVLTFAPTRSGKGVGLVVPTLLSWPASAVIHDIKGENWQIT
                     AGWRSRFSHCLLFNPTDASSAAYNPLLEVRRGAHEVRDVQNIADILVDPEGALEKRNH
                     WEKTSHALLVGAILHVLYAGEDKTLRGVANFLSDPACPFELTLHRMMTTRHLGDVPHP
                     VVASAAREVLNKSDNERSGVLSTAMSFLGLYRDPTVAAVTSRCDWRIADLIASASPVS
                     LYLVVPPSDISRTKPLIRLILNQIGRRLTESLDGSDGIERRHKLLLMLDEFSALGRLD
                     FFETALAFMAGYGIRSFLIAQSLNQIDKAYGQNHSILDNCHVRVTFATNDERTAKRIS
                     ETLGTATELRAQRNYAGHRLAPWLGHLMVSRQETARPLLTPGEVMQLAPDESVVMVSS
                     VAPIKAKKLRYYADANFKQRVLPPPALTAGRYADVPPARPDDWSGLAIPAVPAAQATA
                     SADDLGGSDDGGPRRQPELSETVAYDPEPDYTPSDLALLDDDDMPPLLPGQFDPALQR
                     TARLASLDPNDGIDI"
     gene            1743..2231
                     /locus_tag="VIO83_09685"
     CDS             1743..2231
                     /locus_tag="VIO83_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEY8331583.1"
                     /translation="MSQYRLNLFIQHEHAKRLDELAAKKGVSKSSIVAAALASWLSPD
                     AGDQREAAIAKRLDRLSRQAERLERDQNIQIETLALFVRYYLTVSTPVPEAHQDAARA
                     QGKARFEQFVEQLGRHLLRGRSLVRDVVEELHPQDREFEMRMDDAAAMAAAHERTAER
                     AS"
     gene            2228..>2832
                     /locus_tag="VIO83_09690"
     CDS             2228..>2832
                     /locus_tag="VIO83_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY8331584.1"
                     /translation="MSAVPQIPPEPRSSAAASQDRRIQMLRTAMGPVIAAALEDPDVV
                     EVMLNPDRTLWVDRLSSGRAPLGIELPEADGERIIRLVAAHVGAEVHRGQPLLTAELP
                     ETGERFEGILPPAAPGPAFALRKRAVSIIGLDRYVADGILTAGQAEFLRHAVRERHNI
                     LIAGGTSTGKTTLANALLAEIAATGDRVLVLEDTIELQCAAR"
BASE COUNT          489 a          966 c          906 g          471 t
ORIGIN      
        1 ctggccgtgg tggtcgccat cgccatgtcg gtgtggcgct cgcgccaggc gcgcaaagtg
       61 acgacctatg gctcggcccg ctgggccgat gtcgccgaca ttcgcaaggc cgggctgacg
      121 cagcccgccg gcgtctttct cggccagcat gacggccact acctgcggca cgaagggccg
      181 gaacacgtcc tgacctttgc gcccacgcgc tcgggcaagg gtgtgggcct ggtggtgccg
      241 acgctgctgt cctggccggc atccgccgtc atccacgaca tcaagggcga gaactggcag
      301 atcaccgctg gctggcgcag tcgatttagc cactgcctgc tgttcaaccc gaccgatgcc
      361 agttcggcgg cctacaaccc gttgctggaa gtgaggcgcg gcgcgcacga ggtgcgcgac
      421 gtgcagaaca tcgccgatat cctggtcgat cccgaagggg cgctggagaa gcgcaaccac
      481 tgggagaaga ccagtcatgc gctgctggtc ggcgcgatcc tgcatgtgct ctacgcggga
      541 gaggacaaga cgctgcgcgg cgtggcgaac ttcctcagtg acccggcttg cccgttcgag
      601 ctgacgctgc atcggatgat gaccacgcgg catcttggag atgtaccgca tcccgttgtc
      661 gcgtccgccg cccgcgaagt gctcaacaag tcggacaacg agcgttccgg cgtgttgagc
      721 accgccatgt cgttcctcgg cctgtaccgt gatccgaccg tggccgcagt cacgtcgcgc
      781 tgcgactggc gcatcgccga tctgatcgcg tctgcatccc ctgtgtcgtt gtatctggtg
      841 gtgccgcctt cggacatcag ccgcaccaag ccgctgatcc ggttgatcct caaccagatc
      901 gggcgacggc tcaccgagtc gctcgatggc agcgacggca ttgagcgccg ccacaagctg
      961 ttgctgatgc tcgacgagtt ctcggctctg gggcgcctgg acttcttcga gacggcgctg
     1021 gccttcatgg ccggctacgg catccgcagt ttcctgatcg cgcagtcgct caaccagatc
     1081 gacaaggcgt atgggcagaa ccattcgatt ctggacaact gccatgtgcg cgtgaccttc
     1141 gccacgaatg acgaacgcac ggcgaaacgg atctccgaaa cgctgggcac ggctaccgaa
     1201 ctgcgtgcgc agcggaacta cgcaggccat cgacttgcgc cgtggctcgg ccacctgatg
     1261 gtgtcccgcc aagagaccgc gcggccgcta ctgacgcccg gcgaagtcat gcagcttgcg
     1321 ccagacgaat cagtggtgat ggtgtccagc gtcgcgccga tcaaggccaa gaagttgcgc
     1381 tactacgctg atgccaattt caagcagcgg gtactgccgc cgcctgcgct aacggcaggc
     1441 cgctatgccg atgtgccgcc tgcacgtccc gatgactgga gcggtttggc gatcccggcc
     1501 gtgcctgcgg cacaggccac ggcatctgcc gatgacctgg gtggctccga cgacggcggc
     1561 ccccgccgtc agcccgagct gtccgaaacc gtcgcctacg accccgaacc cgattacacg
     1621 cccagcgact tggcgctgct cgatgacgac gacatgcccc cgctgcttcc cggccagttc
     1681 gaccccgccc tgcaacgcac ggcgcggctg gcatctctcg accccaacga cggaatcgac
     1741 atatgagcca ataccgattg aacctgttca tccagcacga gcacgccaag cgtctggacg
     1801 agctggccgc gaagaaaggc gtgtcaaagt ccagcatcgt cgccgctgcc ttggcgtcct
     1861 ggctgtcacc cgatgccggc gaccagcgtg aggccgccat cgccaaacga ctggatcgcc
     1921 tgtcgcggca ggccgagcgc ctggagcgtg accagaacat ccagatcgag acgctggcgc
     1981 tattcgtccg ctactacctg accgtcagca cgccggtgcc ggaggcccat caggatgcgg
     2041 ctcgcgcgca gggcaaggcg cgcttcgagc agttcgtcga acagttaggc cgccacctgc
     2101 tgcgtggccg cagcctggta cgggacgtgg tggaagaact gcatccccag gatcgtgagt
     2161 tcgagatgcg catggatgac gcggccgcga tggccgccgc ccatgaacgc actgcggagc
     2221 gtgcgtcatg agtgccgttc ctcaaatccc gcccgaaccg cgctcatccg ctgcagcatc
     2281 ccaggatcgc cgcatccaga tgctgcgcac ggcgatgggg ccggtgatcg ccgcagcgct
     2341 ggaagacccg gacgtggtgg aagtgatgct taaccccgac cggacgctgt gggtggatcg
     2401 gctgtcgtct ggccgtgcgc cgctgggcat cgaactgccc gaagccgatg gcgaacgcat
     2461 catccgcctg gtcgccgccc atgtcggtgc ggaggtgcat cgcggccaac cgctcttgac
     2521 cgccgaactg cctgaaaccg gcgaacgctt cgagggcatc ctgccgcccg ccgcacccgg
     2581 cccggccttt gcgctgcgca agcgggccgt gagcatcatc ggtctagatc gctatgtggc
     2641 cgatggcatc ctgaccgctg ggcaggccga gtttctgcgt catgccgtgc gcgagcggca
     2701 caacatcctg atcgccggag gcaccagcac cggcaagacc acgctggcca atgccttgct
     2761 ggccgagatc gccgccaccg gcgaccgcgt gctggtgctc gaagacacca tcgaactgca
     2821 atgcgcggcc cg
//
LOCUS       DATCUN010000251         3622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_49344, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000251 DATCUN010000000
VERSION     DATCUN010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49344"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /gene="phoR"
                     /locus_tag="VIO83_09695"
     CDS             <1..1194
                     /gene="phoR"
                     /locus_tag="VIO83_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEY8331585.1"
                     /translation="GWLAGGRVAAGIALVAVAEVVLLLTRIRHQARAMMTAPTAASPL
                     QHDRFMTRSRRLASSLHDLRNAAGQLPDAVVLLDRQQQIRWFNHAAENLLGLRRPDDR
                     GASLQQRLAGSELAGWLRDGAHEQLTDATAPGRADSQLNVSLLPFGDQEQLLLAHDIS
                     HLNRLEQVRRDFVANVSHELRTPLTVIHGYLELLDPEDVPELAAVLGEMRTQSKRMGQ
                     IVEDLLTLSRLETQHEVIDERVPMAALLATVRKEAEALSQGRHRIVLESTAEADLLGS
                     PKDLHSALSNLASNAVRYTPAGGSITIRWQREPDGAVYSVSDTGFGIPASHLARLTER
                     FYRVSSSRSRDSGGTGLGLSIVKHVLNLHRAQLRIESAPGVGSTFSCHFGAARLLTPG
                     TGDES"
     gene            1246..3339
                     /gene="ppk1"
                     /locus_tag="VIO83_09700"
     CDS             1246..3339
                     /gene="ppk1"
                     /locus_tag="VIO83_09700"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY8331586.1"
                     /translation="MAQPSPATPDATDLTTPGLYLSRELAALEFNFRVLTMARDTAVP
                     LLERLRYLSIVANNLDEFFEVRVAMLKHHHAYGSAAPGPDGIPSGELLARIRSRVLDL
                     VAEVYAAWQEQIGPQLDAEQIHILTRKRWNPRQHRWLRGYFEHEVLPVLSPLGLDPAH
                     PFPRILNKTLNIAVVLRGRDAFGHEGHMALVRAPRSLPRIIRVPAEVSGPGEHFVFLA
                     ELLQAFVDLMFPGLKVVGSYQFRVTRNSELIVEEAEVENLALALSEELVGRGYARPVR
                     LEIANDCPKAITAMLTRNFQLDDADVYRCDGPVNIIRAGLIYDWLDRPELKFPRFNPQ
                     LPPALESGRSKFDLIGQRDVLLHHPYQSFAAVIDLLRQATADPQVLAIKQTLYRAGED
                     TPLVDLLVEAARNGKDVTVVIELRARFDEEANIRLATRLQEAGVQVVYGVVGYKTHAK
                     MMLIVRREGDTLRRYVHLSTGNYHQANSRMYTDIGLMTANPEIGEDLHKVFQQLSGLG
                     PIIELKRLLHSPFTLYPSVLAKIERETAHAQAGRPARIVAKLNALNEARVIEALYRAS
                     QAGVEIDLIVRGACTLRPGLPGISERIRVRSIVGRFLEHSRVYWFANDGAPEIYCASA
                     DWMERNLMRRIEVAFPILDPELAARVFEETLANGLADNTQAWLLGSDGRYVRAEPGDN
                     PPYSAQSGLLERYCQ"
     gene            3425..>3622
                     /locus_tag="VIO83_09705"
     CDS             3425..>3622
                     /locus_tag="VIO83_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEY8331587.1"
                     /translation="MSPAEQIPIRDGELIAAVDMGSNSFHLVVARMEHGEPRVIDRLR
                     DTVRMAAGLRADGTLDAERRAR"
BASE COUNT          577 a         1323 c         1199 g          523 t
ORIGIN      
        1 ggctggctcg ccggcgggcg cgtggcggca ggaatcgccc tggtcgccgt ggccgaagtc
       61 gtcttgctgc tgacccggat ccgtcatcaa gcgcgggcca tgatgaccgc accgactgct
      121 gccagccctc tccagcatga ccgtttcatg acgcgctccc gccgccttgc ctcctccctg
      181 cacgatcttc gcaacgccgc cggccagctg ccggatgcgg tggtgctgct cgaccggcag
      241 cagcagatcc gctggttcaa ccacgccgcc gagaacctgc tcgggctgcg ccgcccggat
      301 gatcgcggcg cgtcattgca gcagcggctg gccggatcgg agctggccgg ctggctgcgc
      361 gacggcgcgc acgagcagct caccgacgcc accgcccccg gccgggcgga cagccagctc
      421 aatgtcagcc tgctgccgtt cggcgaccag gagcaactgc tgctggcgca cgacatcagc
      481 catctgaacc ggctcgaaca ggtgcgccgc gacttcgtgg cgaacgtgtc gcacgagttg
      541 cgcacgccgc tgacggtgat ccacggctac ctcgaactgc tcgacccgga ggacgtgccc
      601 gagctggcgg cggtgctggg cgagatgcgc acgcagtcga aacggatggg ccagatcgtg
      661 gaagacctgc tgaccctgtc gcggctggaa acccagcatg aggtcatcga cgaacgcgtg
      721 ccgatggcgg cgctgctggc caccgtgcgc aaggaagccg aggcgctcag ccagggccgc
      781 catcgcatcg tgctggagtc caccgccgag gcggacctgc tcggctcgcc gaaggacctg
      841 cacagcgcgc tgtcgaacct ggccagcaat gcggtgcgct acaccccggc cggcggcagc
      901 atcacgatcc gctggcaacg cgagcccgac ggcgcggtgt actcggtcag cgacaccggc
      961 ttcggcatcc cggcctcgca cctggcccgg ctcaccgaac gtttctaccg ggtctcctcc
     1021 agccgctcgc gcgacagcgg cggcaccggc ctgggcctgt cgatcgtcaa gcacgtgctg
     1081 aacctgcacc gggcacagct gcgcatcgag agcgcgccgg gcgtcggctc caccttctcc
     1141 tgccatttcg gcgcggcccg cctgctcact cccggcaccg gcgacgaaag ctgaaaccgc
     1201 ggcggcgcgg catgcaatgg cccaggccat gcgccagaat gcggcatggc ccagccctcc
     1261 cccgcaacgc ccgacgccac cgacctgacc acccccggcc tgtacctcag ccgtgaactg
     1321 gccgcgctgg agttcaactt ccgcgtgctg acgatggccc gcgacaccgc cgtgccgctg
     1381 ctggagcggc tgcgctacct gagcatcgtg gcgaacaatc tcgacgagtt cttcgaggtg
     1441 cgcgtggcga tgctcaagca tcaccacgcc tacggctcgg ccgccccggg gccggatggc
     1501 attccttccg gcgagctgct ggcgcgcatc cgcagccgcg tgctggatct ggtcgccgag
     1561 gtctacgccg cctggcagga gcagatcggc ccgcaactcg acgccgagca gatccacatc
     1621 ctcacccgca agcgctggaa cccgcgccag caccgctggc tgcgcggcta cttcgagcat
     1681 gaggtgctgc cggtgctgtc gccgctggga ctggacccgg cgcacccgtt cccgcgcatc
     1741 ctcaacaaga cgctgaacat cgcggtggtg ctgcgcggcc gcgacgcgtt cggccacgag
     1801 ggccacatgg cgctggtgcg ggcgccgcgt tcgctgccgc ggatcatccg cgtaccggcc
     1861 gaggtcagcg gacccggcga gcacttcgtg ttcctcgccg aactgctgca ggccttcgtc
     1921 gacctgatgt tccccggcct caaggtggtc ggctcgtacc agttccgggt cacccgcaac
     1981 agcgagctga tcgtcgagga ggccgaggtg gagaacctcg ccctcgcgct cagcgaggaa
     2041 ctggtcgggc gcggctatgc gcggccggtg cggctggaga tcgccaacga ttgcccgaag
     2101 gcgatcaccg cgatgctgac ccggaacttc cagctggacg acgccgacgt gtaccgctgc
     2161 gacggcccgg tcaacatcat ccgcgcgggc ctgatctacg actggctgga ccggccggaa
     2221 ctgaagttcc cgcgcttcaa cccgcagctg ccgccggcgc tcgagagcgg gcgcagcaag
     2281 ttcgacctga tcggccagcg cgacgtgctg ctgcaccacc cgtaccagag cttcgccgcg
     2341 gtgatcgacc tgctgcgcca ggccaccgcc gacccgcagg tgctggcgat caagcagacc
     2401 ctgtaccgcg ccggcgagga cacaccgctg gtcgacctgt tggtggaggc ggcccgcaac
     2461 ggcaaggacg tcaccgtggt gatcgagttg cgcgcgcgct tcgacgagga ggccaacatc
     2521 cgcctggcca cgcgcctgca ggaggccggc gtgcaggtgg tctacggcgt ggtcggctac
     2581 aagacccatg ccaagatgat gctgatcgtg cgccgcgagg gcgacacgct gcgccgctac
     2641 gtgcacctgt ccaccggcaa ctaccaccag gccaacagcc gcatgtacac cgacatcggc
     2701 ctgatgacgg cgaacccgga gatcggcgag gacctgcaca aggtcttcca gcagctgtcc
     2761 ggcctgggcc cgatcatcga gctgaaacgg ctgctgcatt cgccgttcac gctgtatccc
     2821 agcgtgctgg cgaagatcga gcgcgagacc gcgcacgcgc aggctggccg cccagcgcgc
     2881 atcgtcgcca agctcaacgc gctgaacgag gcacgcgtga tcgaggcgct gtaccgcgcc
     2941 tcgcaagccg gcgtggagat cgacctgatc gtgcgcggcg cgtgcaccct gcgccccggc
     3001 ctgcccggca tttccgagcg catccgcgtg cgctcgatcg tggggcgctt cctcgagcac
     3061 agccgggtgt actggttcgc caacgacggc gcccccgaga tctactgcgc cagcgctgac
     3121 tggatggagc gcaacctgat gcggcggatc gaagtggcgt ttccgatcct cgaccccgag
     3181 ctggccgcgc gcgtgttcga ggaaaccctg gccaacggcc tggccgacaa cacccaggcc
     3241 tggctgctcg gcagcgacgg ccgctacgtg cgcgccgagc ccggcgacaa cccgccgtac
     3301 agcgcgcagt cgggcctgct cgaacgctac tgccaatgaa ccggacgatc cgcatgagcc
     3361 gccgttttct ccctttctcg catgcatgcg agaatccgcg ctttaccgtc gcgggagccc
     3421 gggcgtgagt ccagccgaac agataccgat ccgcgacggc gaactgatcg ccgccgtgga
     3481 catgggctcg aacagcttcc acctggtggt ggcgcgcatg gaacacggcg agccgcgcgt
     3541 gatcgaccgc ctgcgcgaca ccgtgcggat ggccgccggc ctgcgcgccg acggtaccct
     3601 ggacgccgaa cggcgcgccc gt
//
LOCUS       DATCUN010000252        16164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_142262, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000252 DATCUN010000000
VERSION     DATCUN010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 16164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16164
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142262"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1390
                     /gene="tssK"
                     /locus_tag="VIO83_09710"
     CDS             32..1390
                     /gene="tssK"
                     /locus_tag="VIO83_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEY8331588.1"
                     /translation="MSWYNKVAWSEGLFLRPQLFQQQERYLEHLAHKRAAALSPFYWG
                     FSHCSIDAESLSLGKVVLASAAGIFSDGTPFDTPGQTLPPAPLTILPEHLEQVIHLAV
                     PIRTPNGEETSFDEPGGASSASLARFSVFDTELRDANSIGQGPKTVQLSRLRLRLLPQ
                     REMTDAWIGLPLTRVTTLRSDGSIALDGNLVPPVSGYGASGLLTDWLTNLHGLCRLRA
                     QSLAARLSGNDGKSSEAAEVSDFLLLQILNRYEPLLEHWLRVGDTSPEHLYTALRTLT
                     GELSTFVRPSTRRPQEHPAYQHINPYLSFKDLFADVQSLLNDVLVRSAQNIPLASRAN
                     GVRVASVDPSELQGFSNLVFAVAAQTPPDLLASQFPAQCKVGPSDRLAELIRSHLPGI
                     PLQALPVPPRQIPFNAGFVYFQIDPRGPLWEHMVRHGGLAMHVAGDFPGLRMELWGVR
                     EK"
     gene            1419..3983
                     /gene="tssI"
                     /locus_tag="VIO83_09715"
     CDS             1419..3983
                     /gene="tssI"
                     /locus_tag="VIO83_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEY8331589.1"
                     /translation="MLSIRTLTASSEAIPMVLGRSALEPVRFSGREGLNSLFEYELLL
                     KTPDGLNLGASGAIDFDLDAFIGREISCTIQLDGMGEFIPGSVGASTDGVGAGARQIN
                     ALITEAELWGEEGRHVQYKLTLRPWLHLTTLNSDARIYQNKTVVQIFDDLLGNYPFPV
                     DKRLIESYAPRDYQTQFNESDHAFFSRLTQENGISYFFQHSEGKHRLVLIDNLGGYTK
                     NPSVAYQEVEYHPPGWKTDAEYISSFVPHHQLTSGRYTTRDYDYTRPRADLSVTRSEP
                     RPTGRADSEVYQWHHGTAGAHYAQPRAGTADANDPHAEGRNLALLRMQALRTHGARAK
                     ASGNLRGMVPGCFFKLARHPRDKANIDYLILETRFLVEDVAQDSQIKDAAPGRKQHWK
                     IEVDFSAHPMSEPLRPALTQPKPYSHGPQVALVVGPADQSLWTDELGRIKVQFPWDRV
                     GGKDQHSSCWIRVSSPWAGNQLGGIHIPRIGQEVVIDFIGGDPDLPVCTGRLHNQNNL
                     PPWDLPGQSALSGFRSRELKKEGGNSAAGRSNHLILDDTAGKIQAQIKSDHQHSQLSL
                     GAITRIEDNAGCKDPRGEGFELRTDGHGVVRAKDGMLLTTEPRDGAARHAKDLGETVQ
                     RLTQAREQHEDLAQAAQQAKAQDGAADQSDVTRVLKAQNDAIMGTGGESGGFPELEEP
                     HLVLASPAGIETTTPQSTHIASGEHIALTSGKHLSLSAAASLLASAKEAIRLFAYNAG
                     MRLVAANADIDIRALKNNINLLAKLEITQTANKITITAKEEVLINGGTSYSRWNNAGI
                     VHGTKGAWIEHASQHSMPGPDSLPVPGVNFPEPKYTAVCKECLLKAMASGAPFGVR"
     gene            3983..4915
                     /locus_tag="VIO83_09720"
     CDS             3983..4915
                     /locus_tag="VIO83_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HEY8331590.1"
                     /translation="MQRSFLQDPLPVETLHQVRTWHGKLDAQAPGGDSVWAVVDGALL
                     GTDGTRRLRAAYGTAVRAFDETPLAAYEELGLLLWQWSTMDARDGLESLRTVMDDRPG
                     VSFVRIRAGRPDALGRILAWLAGGTTADGMPFYMRVGDSRVLSSFLRHMQPAQQAHLQ
                     TVVREWVWPDRSGQAQSMVIQVGEPPLAEPAAALVIDDAQYAALLDDAAADILHAALR
                     SIESSWHDKRTGAQLHAWLQHVVQRADALGITRQRDQLAFASLALRVSGEFETAPELG
                     ETWLRVRSGVATLPDEVEHWTPAQWAAMERFWHV"
     gene            4964..5497
                     /locus_tag="VIO83_09725"
     CDS             4964..5497
                     /locus_tag="VIO83_09725"
                     /inference="COORDINATES: protein motif:HMM:NF023173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HEY8331591.1"
                     /translation="MNPPSVFFRWLTLACALSMVLALSGCEPPAPRKPQDMAVGIMGY
                     NFTAEGVQEYYVNGSRGSNLPPYGGGGSTSCCVRLPGEWTPEVTVKVDWTIGDWKVPY
                     AQIAHLSTKEQMACCWAERTLSKTVPVERYGKEGGGVQVFFLPNDEIEVWVTDLGLGH
                     ARHPSGVPYPKNPADTE"
     gene            5546..5833
                     /locus_tag="VIO83_09730"
     CDS             5546..5833
                     /locus_tag="VIO83_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331592.1"
                     /translation="MAVEPLPAAQSADRPFSCLDQMKRIKAAPHTDDYKSKTLPYSTV
                     IHVGIFFDGTNNKKRDQEAVFDCVFSCPSSMHRTLKTSKRIGSQRTSLLCK"
     gene            5836..6369
                     /locus_tag="VIO83_09735"
     CDS             5836..6369
                     /locus_tag="VIO83_09735"
                     /inference="COORDINATES: protein motif:HMM:NF023173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HEY8331593.1"
                     /translation="MNSIRTLFRWLTLACALSIVLALSGCEPPAPRKVQDMAVGITGY
                     NFTAEGVQEYYVNGSRGSNLPSYGGGGKTSCCVRLPGEWTPEVTVKVDWTIGHWTTAY
                     ETRKHLSIPEQIQCCFTERTLSKTVPVERYGEEGGRVQVFFLPNDEIKVWVSNLGLGH
                     ARHPSGVPYPRNPANPE"
     gene            6418..8373
                     /locus_tag="VIO83_09740"
     CDS             6418..8373
                     /locus_tag="VIO83_09740"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEY8331594.1"
                     /translation="MAVEHLPPAQSADHPFSCLDQMKRIKAAPHTDDYKGKTLPCSTV
                     IHVGIFFDGTNNNRKRDKVDVLKKTNDPSKCSHSNVVVLYDAFKDVPDDGYFSFYIPG
                     VGTPFDEIGEKTETPDGKAKATGGDARINWAMLQLLNAMHYSLTREQLVDDPVAGRLV
                     NTLPLHAGQVPHANLEGKRNYFTGNGPGTTGPGGKVAELQRALEAQGPQRKLVQVNLS
                     VFGFSRGSAQARAFCHFVHHILLKKAEGGYTLAGVPLRIQFLGLFDSVASVGLADASP
                     FWRGLGGWANGTLDIPPSVQRTVHLCAAHEIRINFPLSTARLGPSKYPANCVEKVYPG
                     AHSDVGGGYPAGCQGKSVGSRAKLLSQMPLLDMYREAQLSMVPLLTLPQLLQTDDGKT
                     VAADLTIDPDNIRLFNAYRAWSADVGGSIENALHGHMQHYWQWRIQYEGRVKALPCYT
                     LADPQDRIDMHESEGDFMSDVNEALRRAQSDARTPDEPPALRLVESDFLRVRTEFLSR
                     KPPQVPKDVDRFFDQMVHDSHASFYMAGPVTDYDKQQRIRKIRQKVELERINRENLKR
                     VGAVRNATPAQDEAAETWSLSDLERRIYEYQKAHDGAFPVMTDADREQLLAMEDLTTS
                     VAVRMVTNKTRRELGGHVRYRRIFDRS"
     gene            8470..9777
                     /gene="tssL"
                     /locus_tag="VIO83_09745"
     CDS             8470..9777
                     /gene="tssL"
                     /locus_tag="VIO83_09745"
                     /inference="COORDINATES: protein motif:HMM:NF005999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, long
                     form"
                     /protein_id="HEY8331595.1"
                     /translation="MSAAIPDSFDSTIHGNDPATTGAVVDDPMDAPIYTLAPEPPVQQ
                     RLAAVKAAQNPLLEAAQPLLRALADMPAHLDVDLVPVLHRLLEREVTTFQKLCSDAQI
                     KHEHVVAASYALCTALDEAANSTEWGGGGKDGGAGVWAVQQLASGFHGDTKGGDKFFL
                     LVGRLAASPQEHTDLLELLYQILGLGFEGRFSTETNGRRQLETIRHRLLTMLVAARGD
                     VPTELSPHWKGEGAGKFRLLRSIPVWVTVALLALALLGLFAWYKYRLLHTSEDVQQRI
                     AAIGQMRPPPVPPAKPLRLKELLSAEIARGTVSVDEDERHSAVTFKGDDMFVPGQARL
                     SARSVPVLAKVADEINQVAGTVQVIGHSDNRPIKTREFPDNHVLSEKRADAVAQVLQD
                     KGVSPSRLKIEGRGDTVPIADNATAAGRARNRRVDIVVTQGTR"
     gene            9844..13860
                     /gene="tssM"
                     /locus_tag="VIO83_09750"
     CDS             9844..13860
                     /gene="tssM"
                     /locus_tag="VIO83_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HEY8331596.1"
                     /translation="MLAFLAVLLVALAVWFIGPLLAVNGLRPLASVGVRVALIVLLLA
                     FAIVWLASWTFGVVGVPAVCLLIWHAGPLLSVGTAKPLAAVSVRVAVIVAILVIYAIY
                     GLYLLWQALQADDKLIKKILSFGKRDEKDQEAQVGLKTVATTVRSAMSQLRSLRSRGG
                     VWRRIFEGKRYLYELPWYMIVGSSGAGKTTALLNSGLQFPAMRQMGNASKSMALKSPV
                     GTVHCDWWFSNEAVLIDTAGRYTTQEIAPEKDLAEWRGFLGLLRKHRTRAPINGVIVA
                     LNAAELLAPTDIERSAQAELIRDRLAELRQELGIRFPVYVIVTKMDLLKGFKEYFQSL
                     TSEGRTQAWGFTLPYQRTAGAKAADIAGNRQALREQVGAELALLKDRLAAGLRARLSE
                     EFDVDRRRRLFALPQELAGLSMPLAQMIDEIFLDSRFDGTQLHHTLRGVYFTSGAQAD
                     AELAANPDTLLERLARSFSGSREDQSGNEEKSERKRADAKTPTAHQGFFLQDVLTKVI
                     VPEAHLVRPNLGWERRFRVVHLLGHVLAVAVFLWLVGALALSFGNNRRYLDTVSARVQ
                     VLGEQVRSLFADFKPTGVPDVLHAARELPGYAGLDVDNPPGSYLYGLYTVPPILDASA
                     ETYAQLQDHMLLPPILQRMETVLATSVKDGDAKTAYETLRVYKLLHDKARYMQEGGAR
                     DVRNWVLKDWETADSAAIFGGRASMVGHVERLFSGARPVQAASLPNEALVREVQNFLD
                     GHTSTQRVYERAKTAMAPEAPQEFTLVRAVGPQVGTVFSRQGGLPLEKGVPGLFTYDG
                     YHDLFNKRLPEFVAHALEDDTWVMGRGGVTPAFALDSGKTADAAARLQDNPLLDDIRR
                     QYLNEYAQHWESFLASIRTVGGGDASGTSLGFDLSVLRQFAAPDSPLARLARAAARET
                     TLSRPLASRAPEDKSFFDKATDELDKKTREIGKSLGIRREERLEKQLVDDRFAALREV
                     VTGQPDIALTSGAGALTAKPGLEAISGLINEFYTLLVVADTALTAGSLPPGGAEVGAR
                     LKLEAGKLPAPFREVLTALATSGGDKVVLGSQDILRKQAQLQLDRIMGLMAMQVSEPC
                     KRGVEGRYPLAAVSQDAAVEDFAQVFAVGGAADEFFTKYLQPFVDTSVRPWRYKNPDT
                     ANAMVGAENIAAGTPPAPVATGPTLLGELLKLLAQSGPNLDAFYRAQQIRDLFFREAG
                     GRKLGWKVDMKVLDLDPTITDLVMDIDGQGQRYIHGPVQSFTVNWPGPRGGSMAELAA
                     NPRISGATSTVSTSGPWALFRLLDKGRIVVGATPGRATVEFSFDGRKALLDLSSGSQP
                     NPLNSDLLKGFRCPGRSA"
     gene            13874..14614
                     /locus_tag="VIO83_09755"
     CDS             13874..14614
                     /locus_tag="VIO83_09755"
                     /inference="COORDINATES: protein motif:HMM:NF021393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TagF domain-containing protein"
                     /protein_id="HEY8331597.1"
                     /translation="MRQALIASCLITPPAIWGKLPAYADFVRSGMRHGESEGWRPWLA
                     QQGDTFGASDAAVLPAAFVLTPGTLAFAPRRFVVGVIAPSCDKVGRRHALLVYQCAHP
                     RWVERHFAHDESWPGDWLFWLARGVDRHTGAARVAEMAALERTVQALWRLHAPTWRDV
                     WRPRPPSPAHLAQRNALAADMLAQLSGAAHRDDAATRLSGVRFLPWADWPRRLFKPRA
                     DSAFWQQDAAGGFVNAAHQLSGLWRRSP"
     gene            14611..15783
                     /locus_tag="VIO83_09760"
     CDS             14611..15783
                     /locus_tag="VIO83_09760"
                     /inference="COORDINATES: protein motif:HMM:NF033419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TagK domain-containing protein"
                     /protein_id="HEY8331598.1"
                     /translation="MSAIGIPAPIRLRLVRSHGDAVDLLLPVPPEGRGLADALELGGE
                     RLNDTQWRLANRCRIVPAGDAWQLVNESHALVCALNGVRVLASTSVAIAAGDMLELAL
                     LRLQVESATAEAMPAAPTAPAMVPQAVHQAVPRVSPDAAADAVDFDLRDLALPTQQTG
                     AQRTPPAGDDPFGVLDIEGAEALPAVDPLAELLGEDPAPRRSRDRRLPQPLDDGAPAD
                     RAPILAGQPSAAMREEALFAELREEFVRVARDPTQLAGRTDWEGLLTRGGAAAPSMDD
                     LVQRGGTIPMVRDIFNPRDPIDRVIEGFDPLGKPMQLAGRDDEEILHLFAPELARKTV
                     AAMPSLTRQEHHHLSPDSHMQLGATRPETSDASRALRLGRSPQPHETDEPQDRQEP"
     gene            15780..>16164
                     /locus_tag="VIO83_09765"
     CDS             15780..>16164
                     /locus_tag="VIO83_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331599.1"
                     /translation="MSLANPPPDSPFGSESFRNWSHARSLQESLGHAEADVRAQPQAP
                     RSRWLLFELLCVLGHWDRALRQLQTWAGLSKEFDSTAHVMRGLIRAERQREAVFAGTA
                     QPATVAAAADMLAPAWMAGMAEALRL"
BASE COUNT         2809 a         5511 c         5299 g         2545 t
ORIGIN      
        1 tccgcatccc tcacctcgaa gtcctcacac catgagttgg tacaacaagg ttgcctggag
       61 cgagggcctg ttcctgcgtc cccagctgtt tcagcagcag gagcgctacc tggagcatct
      121 cgcgcacaaa cgcgctgcgg cgctgagccc gttctactgg ggcttctcgc attgctcgat
      181 cgacgccgaa tcgctctcgc tgggcaaggt ggtgttggcc agtgcggccg gcatcttctc
      241 ggacggcacg ccctttgaca cgccggggca gacgctgccc ccggcgccat tgaccatcct
      301 gcccgagcat ctggagcagg tcatccatct ggccgtgccc attcgcacgc ccaacggcga
      361 agaaaccagc ttcgacgaac ccggcggcgc ctcgagcgca tcgctcgccc gcttctcggt
      421 cttcgacacc gaactcagag acgccaattc gatcggccag ggccccaaga ctgtccagct
      481 gtcgcgcctg cggctgcgcc tgctgccgca acgggagatg acggacgcat ggatcgggct
      541 gcccctgacg cgcgtgacca ccttgcgctc ggacggcagc atcgcgcttg acggcaacct
      601 cgtgccgccc gtctctggct atggcgccag cggcctgctc accgactggc tgaccaatct
      661 acatggcctg tgccggctgc gtgcgcaatc gcttgcggcg cggctcagcg gcaacgacgg
      721 caagtcgagc gaggccgccg aggtctccga cttcctgctg ctgcagatcc tcaaccgcta
      781 cgagcctctg ctcgagcatt ggctgcgcgt gggcgacacc tcgcccgagc acctctacac
      841 cgcgctgcgc acgctgacgg gcgagttgtc caccttcgtc cgcccgagca ctcgccggcc
      901 gcaggaacat cccgcctacc agcacatcaa tccgtacctg tcgttcaagg acctgttcgc
      961 cgatgtgcag tcgctgctca acgacgtgct ggtacgcagc gcgcagaaca ttccgctggc
     1021 cagccgcgcg aacggcgttc gcgtcgcgag cgtcgatccg tcggaactgc agggcttctc
     1081 gaacctggtg tttgcggtcg ctgcgcagac gccgcctgac ctgctcgcca gtcagttccc
     1141 agcgcaatgc aaggtgggcc cgagcgaccg actcgccgag ctcatccgtt cgcatcttcc
     1201 cggcatcccg ctccaggcgc tcccggtgcc gccgcgccag atcccgttca acgctggctt
     1261 cgtctacttc cagatcgatc cgcgcggccc gttgtgggag cacatggtca ggcacggcgg
     1321 actggccatg catgtcgccg gcgacttccc cggcctgcgc atggagctgt ggggcgtgcg
     1381 cgaaaaatga cttcctcatc ggcgccaagg agcatcgcat gttgagcatc cgtaccctca
     1441 ccgcctcgtc cgaggctatt cccatggtcc tcgggcgttc ggccctcgag cctgtgcgct
     1501 tctctggccg cgagggcctc aacagcctgt tcgagtacga actgctgttg aagacccctg
     1561 acggcttgaa cctgggtgca agcggcgcca tcgacttcga cctcgatgcg ttcatcggcc
     1621 gcgagatcag ctgcaccatt cagctcgacg gcatgggtga attcattccc gggtcggtcg
     1681 gcgccagtac cgacggggtg ggcgccggag cgcgccagat caacgccctg atcaccgagg
     1741 ccgagctctg gggcgaagag ggccgccacg tccagtacaa gctcacgctg cgtccatggc
     1801 tccatctgac gaccctcaac agcgacgccc gcatctacca gaacaagacc gtcgtgcaga
     1861 tcttcgatga tctgctgggc aactacccgt tcccagtcga caagcgcctg atcgagagtt
     1921 acgcccctcg ggattaccaa acgcagttca acgaaagcga tcacgccttc ttctcgcgcc
     1981 tgacccagga aaacggcatc agctacttct tccagcacag cgaaggcaag catcggctcg
     2041 tcctcatcga caacctgggc ggctacacga agaacccgag cgtcgcctac caggaggtcg
     2101 aataccatcc gcccggctgg aagaccgatg ccgagtacat cagcagcttc gtgccgcatc
     2161 accagctcac cagcggcaga tacacgacca gggactacga ctacacgcgc ccgcgagccg
     2221 acctgagcgt cacccgcagc gaaccccggc ccaccggccg ggccgacagc gaggtttacc
     2281 agtggcacca cggcacggca ggtgcgcact acgcccagcc tcgagcggga actgcagacg
     2341 ccaacgaccc gcacgccgag ggccgcaact tggcgttgct gcgcatgcag gccctgcgca
     2401 cccatggtgc gcgagcgaag gccagcggca accttcgcgg catggtcccc ggttgcttct
     2461 tcaagctcgc caggcacccg cgcgacaagg cgaacatcga ctacctgatc ctggaaacgc
     2521 gcttcctcgt cgaagacgtg gcgcaagaca gccagatcaa ggacgcggcc cctgggcgca
     2581 aacagcactg gaagatcgag gtcgacttct cggcacaccc gatgagcgag ccgttgcgcc
     2641 cggcgctgac gcaaccgaaa ccctacagcc atggcccgca ggtcgcgctg gtcgtcggcc
     2701 ccgctgacca gagcctctgg accgacgaac tggggcgtat caaagtccag ttcccatggg
     2761 atcgcgtggg cggaaaggac cagcacagca gctgctggat ccgggtcagc tcgccctggg
     2821 ccggcaacca gctcggcggc atccacatcc cgcgcatcgg gcaggaagtc gtcatcgact
     2881 tcatcggcgg cgaccctgac ctgccggtct gcaccgggcg cctgcacaac cagaacaacc
     2941 tcccgccgtg ggacttgccc ggccagagtg ccttgtccgg ctttcgatcc agggagctga
     3001 agaaggaagg cggcaacagc gcggcgggcc gctcgaacca cctgatcctg gacgacaccg
     3061 ccgggaagat ccaggcgcag atcaagagcg accaccagca cagccagctg agcctgggcg
     3121 ccatcacccg catcgaagac aacgcaggct gcaaggaccc gcgcggcgaa ggcttcgagc
     3181 tgcgcaccga tggtcacggc gtggtccgtg cgaaagacgg catgctgctg accaccgagc
     3241 cgcgcgacgg cgcggcccgg catgccaagg acctgggtga aaccgtgcaa cgcctcacac
     3301 aggcgcgtga gcaacacgaa gacctggcgc aggcggcgca gcaggccaag gcccaggacg
     3361 gcgctgccga ccaaagcgat gtgacgcgtg ttctgaaggc gcagaacgac gccatcatgg
     3421 gcacaggcgg cgaatccggt ggctttcccg agctcgaaga acctcacctg gtcttggcca
     3481 gccctgcggg catcgaaacc acaacaccgc agagtacgca cattgcgagc ggtgaacaca
     3541 tcgccctcac cagcggcaag catctcagtc tgagcgccgc cgccagcttg ctggccagtg
     3601 ccaaggaggc gatccgtctg ttcgcctaca acgccggcat gcgtctggtc gccgccaatg
     3661 cggacatcga catccgggcg ttgaagaaca atatcaacct gctggccaaa ctcgagatca
     3721 cccagaccgc gaacaagatc accatcaccg ccaaggaaga ggtgctgatc aatggcggca
     3781 ccagctacag ccgctggaac aatgcaggca tcgtgcatgg caccaagggt gcctggattg
     3841 agcacgccag ccagcacagc atgccgggcc ctgacagcct gccagtgccc ggcgtcaact
     3901 ttcccgaacc gaagtacacc gccgtgtgca aggagtgcct gctcaaggcc atggcttcgg
     3961 gtgctccgtt tggggtgcgc tgatgcagcg gtctttcctt caagacccct tgccggtcga
     4021 aacgctgcac caagtcagaa cctggcatgg aaagctcgat gcccaggctc ctgggggcga
     4081 cagcgtgtgg gcagtggtcg acggggccct tctcggcacg gatggcacac gccggctgcg
     4141 ggccgcgtac ggtactgcgg tgcgcgcttt tgacgagacg ccattggccg cctacgaaga
     4201 gttgggcctt ctgctctggc aatggtccac catggacgct cgagacggtt tggagtcgtt
     4261 gcgcaccgtc atggacgacc gacccggggt gagcttcgtg cgcatccgtg ccggccgacc
     4321 tgatgccttg ggccgcatcc tcgcctggct ggccggcggc accaccgcgg acgggatgcc
     4381 gttctacatg cgcgtggggg actctcgcgt cttgagcagc ttcctgcgcc acatgcagcc
     4441 ggcccagcag gcgcacctgc agactgtcgt tcgcgagtgg gtttggcccg accgctcggg
     4501 tcaagcgcag agcatggtga tccaggtcgg cgagccgccg ctggctgagc cagcggcggc
     4561 gctggtgatc gacgacgcgc agtacgccgc cctgctcgat gatgccgcgg cggacatcct
     4621 gcacgccgcc ctgcgttcca tcgaatcgtc ttggcatgac aagcgaacgg gagctcaact
     4681 gcacgcgtgg ctgcaacatg tggtgcaaag agcagatgca cttggcatca cgcgacagcg
     4741 agaccagttg gcattcgcat cgctggctct gcgtgtttct ggcgagttcg aaacggcgcc
     4801 tgaattgggc gaaacctggt tgcgggtacg cagcggcgtg gccaccctgc ccgacgaggt
     4861 cgagcactgg acgcctgcgc aatgggcagc tatggagcgc ttttggcatg tgtgaagttc
     4921 tttcgaccgt tatgcggtgc gtgttgatcc tgaggcatca acggtgaacc cgccgtcagt
     4981 cttttttcgc tggctgaccc tcgcctgtgc gctgtcgatg gtcctcgcgc tgtccggctg
     5041 cgaaccaccg gcgccgcgca agccgcagga catggctgtc ggcatcatgg gctacaactt
     5101 caccgccgaa ggggtgcagg agtactacgt gaacggcagc cgcggttcca acctgccgcc
     5161 gtacggtggc ggtggctcga cctcctgctg cgtgaggctg cccggcgagt ggacgccgga
     5221 ggtgacggtg aaggtggact ggacgatcgg cgactggaaa gtgccgtatg cacagatcgc
     5281 tcacctgtcc acgaaagaac agatggcttg ttgttgggct gagcgcacgc tcagcaaaac
     5341 agtccccgtt gagcgctacg gcaaagaggg cggaggtgtg caggtgttct tcctgcccaa
     5401 cgacgagatc gaggtgtggg tgaccgactt ggggctgggt cacgcgcggc atccgtcggg
     5461 cgtcccttat ccgaagaacc ccgccgatac cgaatagccg cggtagcgat tcaaccaatc
     5521 aacaaataca aaacagggaa ccccaatggc cgtcgaaccc cttcccgccg cgcaaagcgc
     5581 ggaccgtccc ttcagttgcc tggaccagat gaagcgcatc aaggctgcgc cgcacacgga
     5641 tgactacaag agcaagacgc tcccgtactc caccgtgatc cacgtgggca tcttcttcga
     5701 cgggaccaac aacaagaagc gcgaccagga ggccgtcttc gactgcgttt tcagttgccc
     5761 aagctcgatg caccgaaccc tgaagacctc caaacggatt gggtcgcagc gcacttccct
     5821 cctttgcaaa tagccatgaa ctcgatacga accctctttc gctggctgac cttggcctgt
     5881 gcgctgtcga tagtcctcgc gctgtctggc tgcgaaccac cggcgccgcg caaggtgcag
     5941 gacatggctg ttggcatcac gggctacaac ttcacggccg aaggggtgca ggagtactac
     6001 gtgaacggca gtcgcggttc caacctgccg tcgtacggtg gcggcggcaa gacctcctgc
     6061 tgcgtgaggc tgcccggcga gtggacgccg gaggtgaccg tgaaagtgga ctggacgatc
     6121 gggcactgga caaccgccta cgaaacgcgc aagcacttgt ctatcccaga gcagatccag
     6181 tgttgcttca ccgagcgcac cctcagcaaa accgtccccg tcgagcgcta cggcgaggag
     6241 ggcggccggg tgcaagtgtt ctttctgccc aacgacgaga tcaaggtgtg ggtgagcaac
     6301 ttgggactgg gtcatgcgcg acatccatcg ggggttccgt atccgaggaa ccccgccaat
     6361 cccgaatagc cccggtagtg attcaaccaa tcaacaaata caaaacaggg aaccccaatg
     6421 gccgtcgaac accttccgcc cgcccaaagc gcggaccacc ccttcagttg cctggaccag
     6481 atgaagcgca tcaaggcggc accgcacacc gatgactaca aaggcaaaac gctcccgtgc
     6541 tccaccgtga tccacgtcgg catcttcttc gacgggacca acaacaacag gaagcgcgac
     6601 aaggtcgacg tgctcaagaa gacgaacgat ccatccaagt gcagtcacag caatgtcgtc
     6661 gtgctgtacg acgcgttcaa ggatgtcccc gacgacggtt atttttcttt ctacatcccc
     6721 ggcgtcggca cgcccttcga cgagatcgga gagaagaccg agacccccga cggcaaagcc
     6781 aaggcgacag gtggcgatgc gcgcatcaac tgggcgatgc tccaactgct caacgcgatg
     6841 cactattcgc ttactcggga gcaactggta gacgacccgg tcgctggacg cctggtcaac
     6901 acgctcccgc tccacgccgg ccaggtgccc cacgccaacc tcgaaggcaa gcgcaactac
     6961 ttcacgggca acggcccggg cacgaccggc cctggcggta aggtggccga actccagcgc
     7021 gccctcgaag cgcaagggcc tcagagaaag ctggtgcagg tcaacctctc tgtcttcggc
     7081 ttctcgcgcg gatcggcgca ggcgcgggcc ttctgccact tcgtgcacca catcctgctg
     7141 aagaaggccg agggcggcta caccctggcc ggcgtgccgc tgcgcatcca gttcctgggc
     7201 ctgttcgaca gcgtggcctc ggttggcctg gctgacgcct cgcctttctg gcgcgggctg
     7261 ggcgggtggg ccaacggcac cttggatatt ccaccgagcg tgcagcgcac ggtgcacctg
     7321 tgcgcggcgc atgagatccg catcaacttt ccgctgtcca cggcgcgtct cggccccagc
     7381 aaatacccgg ccaattgcgt ggagaaggtc taccccggtg cgcactccga cgtgggcggc
     7441 ggctacccgg ccggttgcca ggggaagtcc gtggggtccc gggccaagct cctgtcgcag
     7501 atgccgctgc tggacatgta ccgggaggcg cagctcagca tggtgccgtt gctgaccctc
     7561 ccgcagttgc ttcaaacaga cgatgggaaa acagttgcgg ccgatctgac catcgatccc
     7621 gacaacatca gactcttcaa cgcctaccgc gcctggtcgg ccgatgtggg cggctccatc
     7681 gagaacgcgt tgcacggcca catgcagcac tactggcaat ggcgcattca gtacgagggc
     7741 cgggtgaagg cgctgccctg ctacaccctg gccgacccac aggatcgcat cgacatgcat
     7801 gagagcgaag gcgacttcat gtccgatgtg aacgaggcgc tgcggcgggc ccaatccgac
     7861 gcgcgcacgc cggacgagcc gccggccctc agactggtcg agagcgactt cctgcgtgtg
     7921 cgaacggagt tcctcagccg aaagccccca caggtgccca aggacgtcga ccgcttcttc
     7981 gaccagatgg tgcacgactc gcatgcgtcg ttctacatgg cggggccggt cacggactac
     8041 gacaagcagc agcggatccg gaagatccgc cagaaagtcg aactcgaacg gatcaatcgc
     8101 gagaacttga agcgggtggg ggcggtgcgc aacgcgaccc cggcgcagga cgaagcggcc
     8161 gagacctggt cgctgtcgga cctggagcgc aggatctacg agtaccagaa agcgcacgac
     8221 ggcgcctttc ccgtgatgac cgatgccgac cgcgagcaat tgctggcgat ggaagacctg
     8281 accacgagcg tggcggtgcg aatggtcacc aacaagaccc ggcgcgagtt gggcggccat
     8341 gtgcgctatc ggcgcatctt cgaccggagc tgacggcatg gccatcccac gcatcgacct
     8401 ccggcgcggc cagcaagtcc tgctcacgcg ctgtggaccg acctcattct tttccaccga
     8461 gcgatatcca tgagcgccgc tattcctgac tccttcgatt cgaccatcca tgggaacgac
     8521 cctgcgacaa ccggcgctgt ggtcgatgac ccgatggacg caccgatcta caccctcgca
     8581 cccgaacccc cggtgcagca gcgcctggcc gccgtgaagg cagcgcagaa cccgctgctc
     8641 gaagcggcgc agcccctgct gcgtgcattg gcggacatgc ctgcgcacct cgatgtcgac
     8701 cttgtcccgg tgctgcaccg cctgctcgag cgcgaggtga ccacgttcca gaaactctgc
     8761 agcgatgcac agatcaagca cgagcatgtg gtggccgcga gctacgcgtt gtgcaccgcg
     8821 ctcgacgagg cggccaacag caccgaatgg ggtggcggcg gcaaggacgg tggcgcgggg
     8881 gtctgggccg tgcagcagct ggcgtcgggg ttccacggcg acaccaaggg cggcgacaag
     8941 ttcttcctgc tggtggggcg cctggccgcg agcccgcagg agcacaccga cctgctcgag
     9001 ttgctgtacc agatcctcgg gctcggcttc gaggggcgct tcagcaccga gacgaatggc
     9061 cggcgccagc tcgagacgat ccgccatcga ctgctgacga tgctcgtcgc cgcgcgcggc
     9121 gatgtgccca ccgagctctc gccgcactgg aagggcgagg gcgcgggcaa gttccggctg
     9181 ctgcgcagca ttcccgtgtg ggtcacggtc gcgttgctgg cgctggcgct gctcggcttg
     9241 ttcgcctggt acaagtaccg gctgctgcac accagcgagg acgtgcagca gcgcatcgcg
     9301 gccatcggcc agatgcggcc gccgcccgta ccgcccgcca agccgctgcg cttgaaggaa
     9361 ctgctcagcg ccgagatcgc gcgcggcacg gtgagcgtcg acgaggacga gcgccacagc
     9421 gcggtcacct tcaagggcga cgacatgttc gtgccgggcc aggcgcggct gagcgccagg
     9481 agcgtgccgg tgctggccaa ggtggccgac gagatcaacc aggtcgcggg cacggtgcag
     9541 gtgatcgggc attccgacaa ccggccgatc aaaacgcgcg agttccccga caaccatgtg
     9601 ctgagcgaga agcgcgcgga cgccgtggcg caggtgctgc aggacaaggg cgtctcgccc
     9661 tcgcggctga agatcgaggg ccgtggcgac acggtgccga tcgccgacaa cgcgacggcg
     9721 gcggggcggg cgcgcaaccg ccgcgtagac atcgtcgtca cgcaaggcac gcgctgagcg
     9781 cgtgcccagg gagtcttcat gcaatacctc aagaattttc tgagtttctt tttctcgcgg
     9841 cagatgctcg ccttcctggc cgtgctgctg gtggcgctgg ccgtgtggtt catcggcccg
     9901 ctgctcgcgg tcaatggcct gcggccgctg gcctccgtcg gcgtgcgcgt cgccctcatc
     9961 gtgctgctgc tggcgttcgc catcgtctgg ctcgcttcgt ggaccttcgg cgtggtaggc
    10021 gtgccggccg tctgcctgct gatctggcat gccggtccgc tgttgtcggt gggcaccgcc
    10081 aagccgctgg cggcggtgag cgtgcgcgtg gcggtgatcg tggcgatcct ggtgatctat
    10141 gcaatctacg gtctctatct gctgtggcag gcgctgcagg ctgacgacaa gctgatcaag
    10201 aagatcctca gcttcggcaa gcgcgacgaa aaggaccagg aggcccaggt ggggctcaag
    10261 accgtcgcga ccaccgtgcg cagcgccatg tcgcaactca ggagcctgcg cagccggggc
    10321 ggcgtctggc ggcggatctt cgagggcaag cgctacctct acgagctgcc ctggtacatg
    10381 atcgtcggca gttccggtgc gggcaagacg acggcccttc tgaattcagg actccagttc
    10441 ccggcgatgc ggcagatggg caatgcctcg aaaagcatgg cgctgaagtc gccggtcggc
    10501 acggtgcatt gcgactggtg gttctccaac gaagcggtgc tgatcgacac cgccggccgc
    10561 tacaccaccc aggagatcgc tcccgagaag gacctcgccg aatggcgagg cttcctgggc
    10621 ctgctgcgca agcaccgcac ccgcgcgccc atcaacggcg tgatcgtcgc gctgaacgcg
    10681 gccgagctgt tggcgccgac ggacatcgag cgctcggcgc aggccgagct gatacgtgat
    10741 cgcctggcgg aactgcggca ggagctgggc atccgcttcc cggtctacgt gatcgtcacc
    10801 aagatggacc tgctcaaggg cttcaaggag tacttccagt ccctcaccag cgaaggccgc
    10861 acccaggctt ggggcttcac gctgccttat cagcgcacgg ccggtgccaa ggccgccgat
    10921 atcgccggca accgccaggc gctgcgcgag caggtcggcg cggaactggc gctgctcaag
    10981 gaccgtcttg ccgccggcct gcgcgcccgg ctgagcgagg agttcgacgt ggaccgccgg
    11041 cgcaggctct tcgcgctgcc gcaggagctg gcgggcctct cgatgccgct ggcgcagatg
    11101 atcgacgaga tctttctcga ctcccgcttc gacggcacgc agctgcacca caccttgcgc
    11161 ggcgtgtact tcaccagcgg cgcgcaggcc gatgccgagc tggcggccaa ccccgacacg
    11221 ctgctggagc gtctcgcgcg cagcttttct ggatcgcgcg aggatcagtc gggcaacgag
    11281 gaaaagagcg agcgcaagcg ggccgacgcc aagaccccga ccgcgcacca gggcttcttc
    11341 ctgcaggacg tgctgaccaa ggtgatcgtg cccgaggcgc atctggtgcg cccgaatctg
    11401 ggctgggaaa ggaggttccg cgtcgttcac ctgctgggcc acgtgctggc tgtcgccgtc
    11461 ttcctctggc tcgtcggcgc gctggcgctg agcttcggca acaaccgccg ctacctcgac
    11521 acggtgagcg cgcgcgtgca ggttcttggc gagcaggtgc gcagtctctt cgccgacttc
    11581 aagccgaccg gcgtccccga cgtgctccat gccgcgcgcg aactgcccgg ctatgcgggg
    11641 ttggatgtcg ataacccgcc gggcagctat ctgtacggcc tgtacacggt gccgccgatc
    11701 cttgacgcct ccgccgagac ctatgcgcag ttgcaggacc acatgctgct gccgcccatc
    11761 ctccagcgca tggagaccgt gctggcgacg agcgtgaagg acggcgatgc caagaccgcc
    11821 tacgaaaccc tgcgcgtgta caagctgctg cacgacaagg cgcgctacat gcaggagggc
    11881 ggcgcgcgcg acgtccgcaa ctgggtgctg aaggactggg agaccgcgga cagtgccgcg
    11941 atcttcggcg ggcgggcgtc catggtgggg catgtggagc ggctcttctc cggcgcgcgg
    12001 cccgtgcagg ccgcctcgct gccgaacgag gcgctggtgc gcgaggttca gaacttcctg
    12061 gacggccaca cttccacgca gcgggtgtac gagcgcgcga agacggcgat ggcgcccgag
    12121 gcgccgcagg agttcacgct ggtgcgtgcc gtcggtccgc aggtcggcac ggtgttctcg
    12181 cgccagggtg gcctgccgct ggagaagggc gtgccggggc tcttcaccta cgacggctat
    12241 cacgaccttt tcaacaagcg cctgccggag ttcgtcgccc atgcgctgga ggacgacacc
    12301 tgggtcatgg ggcgcggcgg cgtgacgccg gccttcgcgc tcgattccgg gaaaacggcg
    12361 gacgccgccg cccggctgca ggacaacccc ttgctggacg acatccgccg ccagtatctc
    12421 aacgagtacg cgcagcactg ggagagcttc ctggcgtcga tccggacggt cggcggcggc
    12481 gacgcctccg gcaccagcct gggcttcgac ctgagcgtgc tgcgccagtt cgcggccccc
    12541 gattcgccgc ttgcgcggct ggcccgtgcc gccgcgcgcg agacgacgct ttcccgtccg
    12601 ctcgccagcc gcgcgccgga agacaagagt ttcttcgaca aggccacgga cgaactcgac
    12661 aagaagacgc gcgagattgg taagtcgctg ggcatccggc gcgaagagcg gctcgagaag
    12721 cagctcgtgg acgaccgctt cgcggcactg cgggaggtgg tcaccgggca gcccgacatc
    12781 gcgctgacgt cgggtgcggg cgcactcacg gccaagcccg gcctcgaagc catctccggc
    12841 ctgatcaacg agttctacac gctgctggtg gtggcggaca ccgcgctcac cgcgggcagc
    12901 ctgccgcccg gcggcgccga ggtgggtgcg cgcctgaagc tggaggccgg caagttgccg
    12961 gcgccctttc gcgaggtgct gaccgccctg gccaccagcg gcggcgacaa ggtcgttctg
    13021 gggtcgcagg acatcctgcg caagcaggcg cagctgcaac tcgaccggat catggggctg
    13081 atggcgatgc aggtcagcga gccgtgcaag cgcggcgtcg aaggccgcta tccgctggcg
    13141 gcggtgagcc aggatgccgc cgtcgaggac ttcgcgcagg tcttcgcggt gggcggtgcg
    13201 gcggacgagt tcttcaccaa gtacctgcag cccttcgtcg acacgagcgt gcggccctgg
    13261 cgctacaaga accccgacac ggccaatgcg atggtgggtg ctgagaacat cgccgccggc
    13321 acaccgccgg cgccggtggc aaccggcccc acgctgctgg gcgaactgct caagctgctg
    13381 gcgcagagcg gcccgaacct ggacgcgttc tatcgggccc agcagatccg cgacctgttc
    13441 ttccgggagg cgggcgggcg caagctcggc tggaaggtcg acatgaaggt gctcgacctc
    13501 gatcccacca tcaccgacct cgtgatggac atcgacggcc agggccaacg ctacatccac
    13561 ggaccggtgc agtccttcac cgtcaactgg ccggggccgc gcggcggatc gatggcggaa
    13621 ctggcggcca acccgaggat ctcgggcgcc acgtcgacgg tttcgacgag tggcccgtgg
    13681 gccctgttcc ggctgctcga caaggggcgc atcgtcgtgg gcgccacgcc gggccgcgcc
    13741 accgtcgagt tcagcttcga cggccgcaag gcgctgctcg acctcagttc cggctcgcag
    13801 cccaaccccc tgaacagcga cctgctcaag ggattccgct gcccggggcg ctcggcatga
    13861 accggaggcc gcgatgcgcc aggccctgat cgcttcctgc ctgatcacgc cgcccgccat
    13921 ctggggcaag ctgcccgcgt atgccgactt cgtgcgcagc ggcatgcggc atggcgagag
    13981 cgaaggctgg cggccatggc tggcgcagca gggcgacacg ttcggcgcct ccgacgccgc
    14041 agtgctgcct gcggccttcg tcctgacgcc gggcaccctg gccttcgcgc cgcgccgctt
    14101 cgtggtgggc gtgatcgcgc cctcgtgcga caaggtgggg cggcgccacg cgctgctggt
    14161 gtaccagtgc gcacatccgc gttgggtcga acggcatttc gcacacgacg agtcgtggcc
    14221 tggcgactgg cttttctggc tggcgcgtgg cgtcgatcgc cacaccggcg cggcgcgcgt
    14281 ggccgagatg gcggcgctcg agcgaacggt gcaggcgctc tggcggctgc atgcgccgac
    14341 ctggcgcgat gtgtggcgac ctcgtccacc gtcgcccgcg caccttgcgc agcgcaacgc
    14401 cctggcggcc gacatgctgg cgcaactctc cggcgccgcg caccgggacg atgccgcgac
    14461 gcggctgagc ggtgtgcgct ttctgccctg ggccgactgg ccgcggcgcc tgttcaagcc
    14521 ccgcgccgac agcgccttct ggcagcagga cgcagcgggc ggtttcgtca atgcagccca
    14581 ccagctgtcc ggcttgtggc ggaggtcccc atgagcgcca tcggcatccc ggcgcccatt
    14641 cgcctgcggc tggtccgcag ccatggagac gccgtcgatc tgcttctacc ggtgccgccg
    14701 gaaggccgcg gtctggctga tgcgctggag ctgggcggcg agcggctcaa cgacacgcag
    14761 tggcgtttgg cgaaccggtg ccgcattgtc ccggcgggcg atgcgtggca gctggtcaac
    14821 gagagccatg cactcgtctg cgcgctgaac ggggtccggg tcctggcgtc gacctccgtc
    14881 gccatcgccg ccggcgacat gctggaactg gcgttgctgc gccttcaggt ggagtcggcc
    14941 acggcggagg cgatgccggc cgctccgacg gcccccgcga tggtgccgca ggcggtgcac
    15001 caggccgtgc cacgggtgtc gccggatgcg gcggccgacg ccgtcgactt cgatctgcgc
    15061 gacctcgccc taccgacgca gcaaaccggt gcgcagcgta cgccaccggc cggtgacgac
    15121 ccctttggcg tgctggacat cgaaggtgcc gaggccctgc cggccgtcga cccgctggcc
    15181 gaactgctgg gggaagaccc ggcaccgcga cgaagccgcg accggcgcct gccgcagccg
    15241 ttggacgacg gtgcccccgc ggaccgcgcc ccgatcctgg ccggccaacc ctccgccgcc
    15301 atgcgcgaag aagcgctgtt cgccgaattg cgcgaggagt tcgtgcgcgt ggcgcgcgac
    15361 ccgacgcagc tcgcgggccg taccgattgg gagggcctgc tgacacgcgg cggtgcggcc
    15421 gcgccctcga tggacgatct cgtccagcgg ggcggaacca ttccgatggt gcgagatatc
    15481 ttcaacccgc gcgatcccat cgaccgggtg atcgagggct tcgacccgct gggcaagccg
    15541 atgcaactcg ctggacgcga cgacgaagag atcctgcacc tgttcgcacc ggaactcgcg
    15601 cgcaagaccg tcgcggcgat gcccagcctg acacggcagg agcaccacca cctgtcgccg
    15661 gacagccaca tgcagctcgg ggccacgcgg cccgagacca gcgatgcctc ccgggcgcta
    15721 cgtcttggac gctctcctca gcctcacgaa accgacgaac cccaagaccg ccaagaacca
    15781 tgagcctcgc caaccctccg cccgacagcc ccttcgggtc cgaatccttc cgcaactggt
    15841 cgcatgctcg gtccctgcag gagtcgctcg gccacgccga agccgacgtg cgcgctcaac
    15901 cgcaggcccc ccggtcccgc tggctcctgt tcgagctgct gtgcgtgctg ggccattggg
    15961 accgcgcgct caggcagctg cagacctggg ccggcctgtc gaaggagttc gacagcacgg
    16021 cacacgtgat gcgcgggctg atccgggccg agcgtcagcg cgaagccgtc ttcgccggca
    16081 cggcgcaacc cgcgacggtg gctgccgccg ccgacatgct ggctccggcg tggatggccg
    16141 ggatggccga ggcgctgcgg ctcg
//
LOCUS       DATCUN010000253         5435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_86035, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000253 DATCUN010000000
VERSION     DATCUN010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86035"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VIO83_09770"
     CDS             <1..773
                     /locus_tag="VIO83_09770"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEY8331600.1"
                     /translation="LTVCLAASPPLGGECPALASPASPARLPLRLLHYRQPVDLEPLP
                     AQPPQQRRIGLRIDPGRSRQPGRRAAHLTLWRVGEIAALVDSGVHAVFKISRGTAMLL
                     EQEAQRLPDAAKAAHTHSGWESSPMEWYLAVLKKYGTFTGRARRKEYWMFFLVNLVAY
                     IALAIIDGLTGTFSAESGVGLLGAIYTLALLIPNLALSARRLHDTDRSAWWMLICLIP
                     VIGAIVFLVFMCLQGSAGDNRFGEDPKASPSGTPGVLA"
     gene            complement(859..1677)
                     /locus_tag="VIO83_09775"
     CDS             complement(859..1677)
                     /locus_tag="VIO83_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease I family protein"
                     /protein_id="HEY8331601.1"
                     /translation="MSNSAAKKLLALVSLLVSALLAIVFTDIQPPATFQDAKQLAWEV
                     HADNPRTFYCGCRYQGNRVDLASCGYQPRKNRQRASRVEWEHVVPAWVIGHQRQCWKQ
                     GGRDHCSANDPLFARAEADLHNLMPAIGEVNGDRSNYPFGMLNAKPHQYGRCQVVVDF
                     AARKVMPREEIRGTIARTYLYMHERYQLRMSKQDRQLYGAWDRMYPVTEQERRRNQAI
                     ACRMGWGNPHVGAVDLRQCGIASQLRGVVAGALGSAKGLPLEQVGEVVMGLLRR"
     gene            1993..3351
                     /gene="yegQ"
                     /locus_tag="VIO83_09780"
     CDS             1993..3351
                     /gene="yegQ"
                     /locus_tag="VIO83_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEY8331602.1"
                     /translation="MTTAFRPELLSPAGTLKSMRYAFAYGADAVYAGQPRYSLRVRNN
                     EFDHANLKLGIDEAHAQGKKFYVVVNIAPHNAKLKTFIKDLEPVVAMGPDALIMSDPG
                     LIMLVRDHFPQQVIHLSVQANAVNWASVEFWRRIGVSRIILSRELSLEEIGEIREQVP
                     SIELEVFVHGALCMAYSGRCLLSGYLNKRDPNQGSCTNACRWEYKVHEGKEDELGNVV
                     HVHEPIPVVQVEPTLGEGAPTDQVMLLEESNRPGEYMEAFEDEHGTYIMNSKDLRAVQ
                     HVERLTKMGVHSLKIEGRTKSHYYVARTAQVYRKAIDDAVAGRPFDMSLMDTLESLAH
                     RGYTEGFLRRHVHDEYQNYQHGYSLSERQQFVGEFTGVRKDGLAEVAVKNRFQVGDLL
                     ELMTPQGNLNFRLEHLLNKKGEASQVAPGDGHIVYLPVPAEVDLNLALLMRHLDGSST
                     RG"
     gene            complement(3536..>5435)
                     /locus_tag="VIO83_09785"
     CDS             complement(3536..>5435)
                     /locus_tag="VIO83_09785"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY8331603.1"
                     /translation="WQCLAFLRAPAERQAAPVWRQTFLLGVAASGITLALVSVLLVPL
                     AAPLLQALICGLLAAAIISASVAYAASLRAFLAFAVPGLLPSSLYLLAGDDVRLQGWG
                     VFGLILLAALLVSAWQINRGARSNLLQRFQNQSLQDFQEQARRAAARLNRELASEVRQ
                     RQAVEEELRAAQRALEQRISEGSQALSASERARREEEAQRLYLTHYDALTGLANRGLL
                     LERLDAVARRSGEPGQELAMLHLDLDRFNRVSASLGQHLADAILVELAQRLIRSVPQA
                     DTIARIAVDEFVILLGGGSGADELEALARRLLAELRQGVTVDGHEVVVSASLGIARLS
                     AAGDVAALFGQASVAMRHAKQLGGNNVQFYRDSLQASSRERLLLEAQLDKALDKGQLE
                     VFYQPKLTLSSDRLLGAEALVRWRHPQFGLVSPASFIGLAEETGQIHAIGEFVLRQAC
                     TQASAWQSVGRAEVRVSVNISMQQLRQGDFVARVAAVLAETGLPAALLELELTESQLS
                     DNVEDVAALFRQLRGLGVRLAIDDFGTGYSSLGYLKHLPVDVVKIDQTFIRELDGSET
                     GGDAAITRAIIVMAHSLGLEVVAEGVEEVAQLDFLRAHGCDEIQGYLFSRPVEAPAMT
                     ELLRARAA"
BASE COUNT          878 a         1921 c         1802 g          834 t
ORIGIN      
        1 gcctgacggt ctgccttgcc gcctccccgc cacttggcgg ggagtgtcct gccctcgcct
       61 ctccagcaag ccccgctcgc cttccgctac gcctgctgca ttaccggcag cctgtcgacc
      121 tcgaacccct tcccgcgcaa ccgccccagc agcgccgcat cggcctgcgg atcgaccctg
      181 ggcgttcgcg acagccaggc cggcgcgcag cacacctcac gctttggcga gtcggcgaaa
      241 ttgcggcgct agtcgacagc ggcgtgcatg cagtcttcaa gatctcgcgg ggcaccgcta
      301 tgctgctgga gcaggaagca caacgtctgc cggatgcggc caaggccgcg cacactcaca
      361 gtggttggga gtcatcaccc atggaatggt atttagcagt tctcaagaag tacgggacgt
      421 tcacgggccg cgcgcggcgc aaggaatact ggatgttctt tctggtgaac cttgtggcgt
      481 acatcgcgct ggccatcatc gacggcctga ccggcacctt cagtgccgag tctggtgtgg
      541 gtctgctcgg cgccatctac acgctggcac tgcttatccc caaccttgcg ctctccgccc
      601 gccgcctgca tgacaccgac cgcagcgcct ggtggatgct catctgcctg attccggtga
      661 tcggcgcgat cgtgtttctg gtgttcatgt gcctgcaggg tagcgctggc gacaaccgtt
      721 tcggcgagga cccgaaggcc agcccgagcg gcacgcccgg cgtcctggcc tgaaccctgg
      781 accagggctg gcccgccagt cctggtctcg ccagccccga tctgcctgcc gctgtcgccg
      841 gcacgccctc ggcgatgctc agcgcctgag cagccccatc acgacctcgc ccacctgctc
      901 cagcggcagg cccttggcgc tgcccagggc accggcgacc accccgcgca actggctggc
      961 gatgccgcac tgcctcaggt cgacggcgcc gacgtgggga ttgccccagc ccatccggca
     1021 ggcgatggcc tggttgcgcc ggcgctcctg ctcggtgacc gggtacatgc ggtcccaggc
     1081 accatacagc tgccggtcct gcttcgacat gcgcagctgg tagcgctcgt gcatgtacag
     1141 gtaggtgcgc gcgatggtcc cgcggatctc ctcgcggggc atgaccttgc gcgccgcgaa
     1201 atcgaccacc acctggcacc ggccgtactg gtggggcttg gcgttgagca tgccgaacgg
     1261 atagttgctg cggtcgccgt tgacctcgcc gatcgccggc atcaggttgt gcaggtcggc
     1321 ctcggcgcgg gcgaacagcg ggtcgttggc gctgcagtgg tcgcgtccac cctgcttcca
     1381 gcactggcgc tggtggccga tcacccacgc cggcaccaca tgctcccact cgacccggct
     1441 ggcgcgctgg cggttcttgc gcggctggta gccacagctg gcgaggtcga cgcgattgcc
     1501 ctggtagcgg cagccgcagt aaaaggtgcg cggattgtcg gcatggactt cccaggcgag
     1561 ctgcttggca tcctggaagg tggcgggggg ctggatgtcg gtgaagacga tggcgagcag
     1621 ggccgacacc agcagcgaaa ccagtgccag cagcttcttt gcagccgagt tgctcatcaa
     1681 cgcgttccct gtaggcgatc aagggggcgg cactctatgc ggccatcggt ggcaaggcaa
     1741 gctggccacc cggcccaggg ccatcgcctg aagccgtccg gcggcagtcc ggggcaaatc
     1801 aagccctgcc gggatcagct tccgtaggag ccagcttgct ggcgatcctt tgattgcagc
     1861 ggaaaatcgc cagcaagctg gctcctaccg tgttcgtgcg attgctctgg ccgcccgccc
     1921 agcagtcgcg cattcctggg caatccgcag taaaatcgcg cactttgtgt acgccttgcg
     1981 agtcgccgcg ccatgaccac cgccttccgt cccgaactgc tctctcccgc cggcacgctg
     2041 aaaagcatgc gctacgcctt cgcctacggc gccgacgcgg tgtacgccgg gcagccgcgc
     2101 tacagcctgc gggtgcgcaa caacgagttc gaccacgcca acctcaagct gggcatcgac
     2161 gaagcccacg cgcagggcaa gaagttctac gtggtggtca acatcgcccc gcacaacgcc
     2221 aagctgaaga ccttcatcaa ggatctcgag ccggtagtgg cgatgggccc ggatgcgctg
     2281 atcatgtccg atccgggact gatcatgctg gtgcgcgacc acttcccgca gcaggtcatc
     2341 cacctgtcgg tgcaggccaa cgcggtgaac tgggcgtcgg tggagttctg gcggcgcatc
     2401 ggcgtcagcc gcatcatcct ctcccgcgag ctgtcgctgg aggagatcgg cgagatccgc
     2461 gagcaggtgc cgagcatcga gctggaggtg ttcgtccacg gcgcgctgtg catggcctac
     2521 tccgggcgct gcctgctgtc cggctacctc aacaagcgcg accccaacca gggctcctgc
     2581 accaacgcct gccgctggga gtacaaggtg catgagggca aggaggacga gctgggcaac
     2641 gtggtccacg tccatgagcc gattcccgtg gtgcaggtcg agccgaccct cggtgaaggt
     2701 gcccccaccg accaggtgat gctgctggaa gagagcaacc ggcccggcga gtacatggag
     2761 gcgttcgagg acgagcacgg cacctacatc atgaactcca aggacctgcg cgcggtgcag
     2821 cacgtcgagc gcctgaccaa gatgggcgtg cactcgctga agatcgaggg ccgcaccaag
     2881 agccactact acgtggcgcg caccgcgcag gtgtaccgca aggcgatcga cgacgcggtg
     2941 gccgggcggc cgttcgacat gtcgctgatg gacaccctcg aatcactggc ccaccgcggc
     3001 tacaccgagg gcttcctgcg ccgccacgtc cacgacgagt accagaacta ccagcacggc
     3061 tactcgctgt ccgaacgcca gcagttcgtc ggcgagttca ccggggtgcg caaggacggc
     3121 ctggccgagg tggcggtgaa gaaccgcttc caggtcggcg acctgctcga gctgatgacc
     3181 ccgcagggca acctcaactt ccgcctcgag cacctgctca acaagaaggg cgaagccagc
     3241 caggtggcgc cgggcgacgg ccacatcgtc tacctgccgg tgccggccga ggtcgacctc
     3301 aacctggcgc tgctgatgcg tcacctggac ggcagcagca cccgcggctg agggcgtttt
     3361 caccgcaatc aggcgcacct gtgcagccgt agggtagaaa acccgcgcag cggttttcta
     3421 cctgccctcc gcgccacggc cagacacctg agggtgggca atcgctgcgc gagttgccca
     3481 ctacggcctg agatcccagc accgcccacg ccatattcag gcgccaaacc cgccctcagg
     3541 ccgcccgggc gcgcagcagt tcggtcatcg ccggggcttc caccggccgg ctgaacaggt
     3601 agccctggat ctcgtcgcag ccgtgggcgc gcaggaagtc cagctgcgcc acctcctcga
     3661 cgccctcggc caccacctcc aggcccaggc tgtgggccat gacgatgatc gcccgggtga
     3721 tcgccgcgtc gccgcccgtc tcgctgccgt ccagctcgcg gatgaaggtc tggtcgatct
     3781 tcaccacatc caccggcagg tgcttgaggt agccgagcga ggaatagccg gtgccgaagt
     3841 cgtcgatcgc caggcgcacg cccagcccgc gcagctggcg gaacaacgcg gccacatcct
     3901 cgacgttgtc cgacagctgg ctctcggtca gctccagctc cagcagcgcg gccggcaggc
     3961 cggtctcggc cagtaccgcg gcgacccgcg cgacgaagtc gccctggcgc agctgctgca
     4021 tggagatgtt caccgacaca cgcacctcgg cccgcccgac cgactgccag gcgctcgcct
     4081 gggtgcaggc ctggcgcagg acgaattcgc cgatggcatg gatctgcccg gtttcctcgg
     4141 ccaggccgat gaagctggcc ggcgacacca ggccgaactg cggatggcgc cagcgcacca
     4201 gcgcctcggc ccccagcagg cgatccgagc tgagggtcag cttgggctgg tagaacacct
     4261 ccagctggcc cttgtcgagc gccttgtcca gctgggcctc gagcagcagg cgttcgcggc
     4321 tgctggcctg caggctgtcg cgatagaact gcacgttgtt gccacccagc tgcttggcgt
     4381 ggcgcatggc cacgctcgcc tggccgaaca gcgcggccac gtcgccggcg gcggacagcc
     4441 gggcgatgcc cagcgaggcg ctgaccacca cttcgtggcc gtcgaccgtc accccctggc
     4501 gcaactcggc cagcaggcgc cgcgccagcg cttccagctc gtcggcgccg ctaccgccgc
     4561 cgagcaggat gacgaactcg tccaccgcga tccgcgcgat ggtgtcggcc tgcggcacgc
     4621 tgcgaatcag gcgttgcgcc agctcgacga ggatggcgtc ggccaggtgc tggccgaggc
     4681 tggcactgac ccggttgaag cgatccaggt cgaggtgcag catggccagc tcctgacccg
     4741 gctcgccgct gcgccgggcg acggcgtcga ggcgttcgag cagcaggccg cggttggcca
     4801 gcccggtcag ggcgtcgtaa tgggtcaggt acaggcgctg cgcctcttct tcccggcgcg
     4861 cccgctcgct ggcgctgagc gcctggctgc cctcgctgat ccgctgctcc agggcgcgct
     4921 gcgcggcgcg cagctcctcc tcgaccgcct ggcgctggcg cacctcgctg gccagctcgc
     4981 ggttcagccg agcggcggcg cgccgggcct gctcctggaa atcctgcagc gactggttct
     5041 ggaaacgctg cagcaggttg ctgcgcgcgc cgcgattgat ctgccaggcg ctgaccagca
     5101 acgccgccag caggatcagg ccgaacacgc cccagccctg caggcggacg tcgtcgccag
     5161 cgagcaggta caggctggaa ggcagcaggc cgggcacggc gaaggccaga aaggcgcgca
     5221 ggctggcggc ataggcgacg ctggcggaga tgatcgccgc cgccagcagg ccgcagatca
     5281 acgcctgcag cagcggcgcg gccagcggga ccagcagcac gctgaccagc gccagggtga
     5341 tgcccgaggc ggcaacgccg agcaggaagg tctgccgcca gaccggggcc gcctggcgct
     5401 cggccggcgc ccgcaggaag gccaggcact gccac
//
LOCUS       DATCUN010000254         1753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_218267, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000254 DATCUN010000000
VERSION     DATCUN010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218267"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VIO83_09790"
     CDS             <1..286
                     /locus_tag="VIO83_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY8331604.1"
                     /translation="PRELPDRPFDYAALKAVYRKAGGKGAWMVNNAYDRPLAEAAVAA
                     GADIVAFGRPFIANPDLVARLEKNAPLNKPDSATFYGGAAAGYTDYPTLY"
     gene            410..1738
                     /locus_tag="VIO83_09795"
     CDS             410..1738
                     /locus_tag="VIO83_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEY8331605.1"
                     /translation="MTRAALEHALPASWVDEVFQTSRQRQYPRELLFSSVVELMMLVS
                     LGLRPSLHAAAKKMAQLPVTLAALYDKVKRTEPQVLRALVQGSAARLEPVVAAVGSQP
                     SLQGWQLRILDGNHLPASDKRLAPLRSHRGAAMPGHTLVVYDPDSALVTDILACEDAH
                     ESERVGAATLLQQAQQGQVWIADRHFCTRTLLQGWQQRQAGFIVREHARHPKLDKTGP
                     WSQAVPTETGQVREQAIELAATQETVVAPPSWRRIEIQLQVPTEAGDQSIALWSNLPA
                     SIDAATIAQLYRKRWRIEGLFGRLESVLHSEIRTLGHPRAALLGFAAAVLAFNVLALL
                     KRCVEQAHCERAPGLDVSSYHLGVHVASDYQGMLIALPSEVWQPWCEASGEEVAQHLL
                     RLAGKVNPRSVATAKRGPKKEKPGGYVAGAVARKQLSTARVLKQAAGTTP"
BASE COUNT          311 a          571 c          590 g          281 t
ORIGIN      
        1 cccccgtgaa ctgcccgacc gtccctttga ctatgccgcc ctcaaggccg tgtaccgcaa
       61 ggccggcggc aagggggcct ggatggtgaa caatgcctac gaccgccccc tggccgaagc
      121 cgccgtggcc gccggcgccg atatcgtggc ctttggccga cccttcattg ccaaccccga
      181 cctggtggcc cggctggaaa agaacgcacc cctgaacaag cccgacagcg ccaccttcta
      241 tggcggcgcc gccgccggct acaccgatta cccaaccctc tactaagagc catctctttc
      301 aaggtgttgc ccctgatccc gcagcagtaa cctgcgcccc acagcgggag agctgcggat
      361 gtggaaagaa gtcttggaga ggtttgagag ccatgcgccg gtgagcgtga tgacccgggc
      421 ggcgttggag catgccttgc cggcgagctg ggtggatgaa gtgttccaga ccagccgcca
      481 acgccagtat cctcgcgagt tgctgttctc cagcgtggtg gagctgatga tgctggtgag
      541 cctggggctg cgcccctcgc tgcacgcggc ggccaagaag atggcgcagt tgcccgtcac
      601 gctggcggcg ctgtacgaca aggtcaagcg cacagaaccc caggtgctgc gggctctggt
      661 gcaaggcagc gcggctcgcc tggagccggt ggttgctgcg gtaggcagcc agcccagcct
      721 gcagggctgg caactgcgca tcctcgatgg caaccatttg ccagccagtg acaaacgcct
      781 ggcgccactg cgctcgcacc ggggagcggc catgccgggc cacacgctgg tggtctacga
      841 ccccgacagc gccctggtca cggacatcct ggcctgcgag gacgcgcacg agagcgaacg
      901 cgtaggcgcg gccaccttgc tgcagcaggc ccagcagggc caggtgtgga ttgccgaccg
      961 ccacttttgc acccgcaccc tactgcaggg ctggcaacag cgccaagcgg gctttatcgt
     1021 gcgtgagcac gccaggcatc cgaagttgga caagaccggc ccctggagcc aagccgtgcc
     1081 cacagagacc gggcaggtgc gtgagcaagc cattgagctg gcggccaccc aggagacggt
     1141 ggtcgccccg ccgtcctggc gccgcatcga gatccagttg caggtgccca ccgaagcggg
     1201 tgaccaaagc attgccttgt ggagcaacct gccggcgagc atcgacgcgg ccaccattgc
     1261 gcagttgtac cgcaagcgct ggcgcatcga gggcttgttc ggacggctcg aatcggtgct
     1321 gcacagcgag atccggacct tgggccaccc gcgtgcggcg ctgctggggt ttgctgcagc
     1381 agtgctggcg ttcaacgtgt tggcgctact caagcgctgc gtggagcagg cgcactgcga
     1441 gcgagcgcct ggtctggatg tttcgagcta tcacctgggc gtgcatgtgg ccagcgacta
     1501 ccagggcatg ctcattgcgc tgccatctga ggtgtggcaa ccgtggtgcg aggcgtcagg
     1561 cgaggaggtt gcgcagcatc tgctgcgcct ggcagggaag gtcaacccgc gcagtgttgc
     1621 cacggccaag cgcggcccca agaaggaaaa gcctggggga tacgtggctg gcgctgtggc
     1681 tcgcaagcag ctgtccaccg ctcgtgtcct caaacaggct gcggggacaa caccttgaaa
     1741 gggatgcccc tta
//
LOCUS       DATCUN010000255         7158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_161987, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000255 DATCUN010000000
VERSION     DATCUN010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161987"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VIO83_09800"
     CDS             <1..822
                     /locus_tag="VIO83_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007994903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8331606.1"
                     /translation="GGIGVGRMSYGSDITIDPGVLDTFSLVMMPIRGQEVIEFGSRQI
                     CSTPSQATVLNAHLPCRIHHHKDTEKLIMRVDRSLLERICQQHLGRTLTKPVEFKPDM
                     PLDTAEGQRWMRMVGWIYDNLSHDEGSLPPLLAAQFEQAMVTALLTCQPNNYSAELCS
                     EEGHSIAPSFVKRVERYIEEHAHEAISIVEMAEHVGVSSRSLFAGFRRFRNTSPMLYL
                     KEVRLRRVHEELQQSSAGSTTVTAVAFRWGFSHLGHFTTDYKRRFGESPSETLAR"
     gene            983..2317
                     /locus_tag="VIO83_09805"
     CDS             983..2317
                     /locus_tag="VIO83_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8331607.1"
                     /translation="MSDQPIVRRRQVKTGISDARANNAKTQSQYQPYKDAAWGFINHW
                     YPALFTHELGEDQVEGIQICGIPIVLRRVDGKVFALKDQCLHRGVRLSEKPMCFNKST
                     ISCWYHGFTFDLESGKLATIVANPDDKLVGTTGITTYPVHEVNGMIFVFVREDDFPDE
                     EVPPLASDLPFRFPENSDRFPHPLWPASPSVLDDNAVVLGMHRTGFGNWRIACENGFD
                     NGHIFVHKDNSIVHVNNWVLPLGLLPVGDDAIKLVEDDNGPKGMMQMMFTENWQPVLE
                     NEELGLKVDGVNGRYYRTSVVLPGVLMVENWPQDHVVQYEWYVPITDDTHEYWEILVR
                     VCNTPEERAKHEYRYKTVYEPLCLHGFNDCDLYAREAMQNFYADGTGWDDEQLVATDI
                     SPITWRKMASRWNRGIAKPGRGVQGSVKDLSLIFKQTADGHRPGYKVKKIEE"
     gene            2374..3237
                     /locus_tag="VIO83_09810"
     CDS             2374..3237
                     /locus_tag="VIO83_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009868030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8331608.1"
                     /translation="MHNDVFSRSHFVTVDGVRLHYRVVDGPADSLPVIFTHGGGPGST
                     AWSNFRLSAEAFAARHTCYFLDFPQFGGSDMVAVEGPVFSWHARKLLGFMDALGIRQA
                     HLVNQSFGGCVAIRFAADHPERVGRLLLIGSQPVVRGVMQPLPLFSKHAATLMSDYYL
                     DDGGPSLAKMRALICRYELHDDRKLDEENLRLRYEASCTPAYRDLLALPGAFGERESL
                     MAVFARVRAPTLIVWGLHDWFGGIDVPMLMLNQFADARLHVMGNAAHHLQSECPAEFN
                     ALATTFIEGHL"
     gene            3234..4637
                     /locus_tag="VIO83_09815"
     CDS             3234..4637
                     /locus_tag="VIO83_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008047619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY8331609.1"
                     /translation="MTSSSDLHYLEMHELSLLIRTRKLSPVEVTEAQLARIDQLDGAL
                     HSYARVTPELALAQARQAEEELARGHCRSPLHGIPLAVKDLFHTAGIPTAAGMAIHRN
                     FIPQQDATVVTRLREAGSVLLGKLQMTEGAFATHHPAFTAPVNPWGAGHWAGASSSGC
                     GVATAAGLCYAALGTDSGGSIRFPCAANGLTGLKPTWGRVSRHGTFELAASLDHVGPL
                     TRSARDALFLLSTIAGHDPLDPTSLPSVCLEIPSVEESFRGLRIGIDEAWLSDGVDEE
                     IVEALEEVLAMIRDGGGTLLRVRMPDTRAVSANWEKHCGVQTAVAHAATYPSRAGEYG
                     PALSRLIDGGRALSGMDYQRVLLDAQRFHGELECVLNEVDLLLAPVQPYAAPTHAQLG
                     ALAQDPEANRRLIQFTAPFNVTGHPCLSLPIGFTAAGLPIGGQFIAGKGGEGVLCRAG
                     MALQKVSDWHKIRPGSF"
     gene            4928..5956
                     /locus_tag="VIO83_09820"
     CDS             4928..5956
                     /locus_tag="VIO83_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008047617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-hydroxylating dioxygenase ferredoxin
                     reductase family protein"
                     /protein_id="HEY8331610.1"
                     /translation="MKDAIHQVTVNFADGVSRSFAVPAEASILDAAIEAEIPLLYQCR
                     SGGCSSCVAQLAEGDARTRAGASSSLLASEYAAGQRLLCLCQAQSDCTFTVPYGSEVG
                     SSVAVEVHAFIDSIERIAVNVVRLTLELADGDWLEFRPGQFVQIEVPGFGVLRSYSPS
                     STLADLPKLQLLVRLLPGGAMSSYLEEKAQIEEVLTLRGPYGAFFLREEHKRAPHIFV
                     AGGTGLAPILSMIDTLRQNGGKKPPMLLSFGCATPEVLFGLDDIELRQQWLPSLEARI
                     CVDREPTGNLHHGSPVSALREGDVVSPDTVAYLCGPQPMINAATARLIELGVAAENIF
                     SEQFVASH"
     gene            5967..>7158
                     /locus_tag="VIO83_09825"
     CDS             5967..>7158
                     /locus_tag="VIO83_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEY8331611.1"
                     /translation="MQPTVSVPFSAEQMMWYEQACARIDPKRLQNLLFELTNIHSPTG
                     AAGAASQFIARHLESVGLSARYQPMSPISGNVLAERRGSGGGASVLLYSPIDTHLEEG
                     DEAQEILTGKAPEVDMQITARMIDDWVFGLGASNPKGMVATLTEVATALIEAEVPLIG
                     DLLVGMADGGMPVDIAGRDHAGMSNGVFHLLNRGGSPDFAVIMKPWNWVYHEEPGMAW
                     FKLRVHGTLGYAGVPRDIPGFRSSVVPAATVIQELERWIIDYAERNTSGQIKPHGWIA
                     GVRGGDPQRPAFPSAVTEIFFDVRVNPRTSPAAVKAQFAAFVRELQAQRPELDLDWEM
                     YGSVAGGSTDPQNWIIQSCQRGWEHIEHRPHGQADLLGGQTDGAALRRLGIPTARIGW
                     PWPAQ"
BASE COUNT         1162 a         2532 c         2347 g         1117 t
ORIGIN      
        1 ggcggcatcg gcgtggggcg catgagctac ggcagcgaca tcaccatcga ccccggcgtg
       61 ctcgacacct tttctctggt catgatgccg atccgcggcc aggaggtgat cgagttcggc
      121 agccggcaga tttgctcgac acccagccag gccacggtgc tcaacgccca cctgccgtgc
      181 cgcatccacc atcacaagga caccgaaaaa ctgatcatgc gcgtcgatcg cagcctgctc
      241 gagcgcatct gccagcagca cctggggcgc accctgacca agccggtgga gttcaagccg
      301 gacatgccgc tggataccgc ggaagggcag cgctggatgc gtatggtcgg ctggatctac
      361 gacaacctgt cccacgacga gggcagcctg ccgccgctgc tcgccgcgca gttcgagcag
      421 gcgatggtca ccgcgctgct gacctgccag cccaacaact acagcgccga actctgcagc
      481 gaagaggggc attccatcgc gccgtccttc gtcaagcggg tggagcgcta catcgaggag
      541 cacgcccacg aggcgatctc catcgtcgag atggccgagc acgtcggcgt cagcagccgc
      601 tcgctgttcg ccggtttccg ccgcttccgc aacacctcgc cgatgctgta cctgaaggag
      661 gtgcgcctgc gccgcgtgca cgaggagctg cagcagagca gcgccggcag caccacggtc
      721 acggcggtgg ccttccgctg gggcttcagc catctgggcc atttcaccac cgactacaag
      781 cgccgcttcg gcgaaagccc ctcggaaacc ctcgcgcgct gagcgcacgg acagggatgt
      841 ccagccgcac tgattttctg cactgccggg ataaccccct cggtcattgg agcgtcagcc
      901 cgctccccgc ttcttaggat gactctcaag ggtgccgcac agggcggtac ccactcttca
      961 gtcagccttc cgggggtttc caatgtccga ccagccgatc gtccgtcgcc gccaagtcaa
     1021 gaccggcatc agcgatgccc gcgccaacaa cgccaagacc cagagccagt accagccgta
     1081 caaggatgcc gcctggggct tcatcaacca ctggtacccg gcgctgttca cccacgagct
     1141 gggcgaggac caggtcgagg gcatccagat ctgcggtatc ccgatcgtcc tgcgccgcgt
     1201 cgacggcaag gtgttcgccc tcaaggacca gtgcctgcac cgcggcgtgc gcctgtccga
     1261 gaagccgatg tgcttcaaca agagcaccat ctcctgctgg taccacggtt tcaccttcga
     1321 cctggagagc ggcaagctgg cgaccatcgt cgccaacccg gacgacaagc tggtcggcac
     1381 caccggcatc accacctacc cggtgcacga agtcaacggc atgatcttcg tcttcgtccg
     1441 cgaggacgac ttccccgatg aggaggtgcc gccgctggcc tccgacctgc cgttccgctt
     1501 cccggagaac agcgaccgct tcccgcaccc gctgtggccg gcctcgccga gcgtgctcga
     1561 cgacaacgcg gtggtgctgg gcatgcaccg caccggcttc ggcaactggc ggatcgcctg
     1621 cgagaacggc ttcgacaacg ggcacatctt cgtccacaag gacaacagca tcgtccacgt
     1681 caacaactgg gtgctgccgc tcggcctgct gccggtcggc gacgacgcca tcaagctggt
     1741 cgaggacgac aacggcccga agggcatgat gcagatgatg ttcaccgaga actggcagcc
     1801 ggtgctggag aacgaggagc tcggtctcaa ggtcgacggc gtcaacggcc gctactaccg
     1861 cacctcggtg gtgctgccgg gcgtgctgat ggtggagaac tggccgcagg accacgtggt
     1921 gcagtacgag tggtacgtgc cgatcaccga cgatacccac gagtactggg aaatcctcgt
     1981 ccgcgtgtgc aacacccccg aggagcgcgc caagcacgag taccgttaca agacggtgta
     2041 cgagccgctg tgcctgcatg gcttcaacga ctgcgacctg tatgcccgcg aggccatgca
     2101 gaacttctac gccgacggca ccggctggga tgacgaacaa ctggtggcca ccgacatctc
     2161 gccgatcacc tggcgcaaga tggcctcgcg ctggaaccgc ggcatcgcca agcccggccg
     2221 cggcgtgcag ggctcggtca aggacctcag cctgatcttc aagcagaccg ccgacggcca
     2281 tcgccccggc tacaaggtca agaagatcga agagtaaggg ggcttcgctg cccggcgcgt
     2341 acgccgggca gcgggcaacc gggagcctgt cgcatgcata acgacgtctt ttctcgcagc
     2401 catttcgtca ccgtggatgg cgtgcgcctg cactaccggg tggtcgacgg tcccgccgac
     2461 agcctgccgg tgatcttcac ccacggcggc ggtcccggca gtaccgcctg gagcaacttt
     2521 cgcctcagtg cagaagcctt cgccgcgcgc catacctgct acttcctcga ctttccccag
     2581 ttcggcggct ccgacatggt ggcggtcgag gggccggtgt tcagctggca cgcgcgcaag
     2641 ttgctgggct tcatggatgc cctgggcatc cgccaggccc atctggtcaa ccagtcgttc
     2701 ggcggttgcg tggcgatccg tttcgctgcc gatcaccccg agcgggtcgg ccgcctgctg
     2761 ctgatcggtt cgcagccggt ggtgcgcggg gtgatgcagc cgctgccgtt gttcagcaag
     2821 catgccgcga ccctgatgag cgattactac ctcgacgatg gcggaccgag cctggcgaag
     2881 atgcgtgcgc tgatctgccg ctacgagctg catgacgatc gcaagctcga cgaggaaaac
     2941 ctgcgtctgc gctacgaggc cagctgcacc ccggcctacc gtgacctgct cgccctgccg
     3001 ggtgcgttcg gcgagcggga aagcctgatg gcggtgttcg cccgggtgcg cgcgccgacg
     3061 ctgatcgtgt ggggactgca cgactggttc ggcggcatcg acgtgccgat gctgatgctc
     3121 aaccagttcg ccgacgcgcg cctgcacgtg atgggcaacg ctgcccacca cctgcagagc
     3181 gaatgcccgg ccgagttcaa tgcgcttgcc acgaccttta tcgagggcca tttatgacca
     3241 gcagcagcga tctgcactac ctggagatgc acgaactgtc gctgctgatc cgtacccgca
     3301 agctgtcgcc ggtcgaggtc accgaggccc agctggcgcg catcgaccag ctggatggcg
     3361 cgctgcacag ctacgcccgg gtcacgccgg agctggcctt ggcgcaggcg cgccaggccg
     3421 aggaggagct ggcgcgtggc cactgccgca gcccgctgca cggcatcccg ctggcggtga
     3481 aggatctgtt ccataccgcc ggcattccca ccgcggcggg catggcgatc caccgtaatt
     3541 tcattccgca gcaggacgcc accgtggtga cgcgcctgcg cgaggcgggc agcgtactgc
     3601 tcggcaagct gcagatgacc gagggcgcct tcgccaccca ccatccggcg ttcactgcgc
     3661 cggtcaatcc gtggggcgcc ggccactggg ccggcgcctc gtccagcggc tgcggcgtgg
     3721 ccaccgccgc cgggctgtgc tacgccgcgc tgggcaccga cagcggcggc tcgatccgct
     3781 tcccctgcgc ggccaacggc ctgaccggcc tcaagccgac ctgggggcgg gtcagtcgcc
     3841 acggcacctt cgagctggcc gccagcctcg accatgtcgg acctctcacc cgcagcgcgc
     3901 gcgacgcgct tttcctgctg tcgaccatcg ccggccacga tccgctggac cccacctcgc
     3961 tgcccagcgt gtgcctggag atccccagcg tcgaggagtc gttccgcggc ctgcgcatcg
     4021 gcatcgacga ggcctggctg agcgatggcg tcgatgagga gatcgtcgag gccctcgaag
     4081 aggtgctggc catgatccgc gacggcggcg gcaccctgct gcgcgtgcgc atgccggaca
     4141 cccgcgcggt gagcgccaac tgggagaagc actgcggcgt gcagaccgcc gtggcgcacg
     4201 cggccaccta tccgagtcgc gccggcgagt acggcccggc gctgagccgg ctgatcgacg
     4261 gcggccgcgc gctctccggc atggactacc agcgcgtgct gctcgacgcg cagcgcttcc
     4321 acggcgagct ggagtgcgtg ctcaacgagg tcgacctgct gctcgccccg gtgcagccct
     4381 atgccgcgcc gacccacgca cagctcggcg cgctggccca ggacccggag gccaaccgcc
     4441 ggctgatcca gttcaccgcg ccgttcaacg tcactgggca cccgtgcctg agcctgccga
     4501 tcggcttcac cgcggccggt ctgccgatcg gcggccagtt catcgccggc aagggcgggg
     4561 agggcgtgct gtgccgcgcc ggcatggcgc tgcagaaggt cagcgactgg cacaagattc
     4621 gtcccggcag cttctgacaa ggactgccct tcatggcccg taccccagcg gtacgggcct
     4681 ttttttgtcc gcgctgcgtc ccccgtgtgt cctggttgct ggccgtcacg atatccgact
     4741 gcggcggcag gaatttgcac tggcgggata acccggtaac tcgcgggagc gcccgcccgg
     4801 gagtcgcttc ctatgatcgg ggcaagtcag cagtaaccgg gcgggccgtt tagccgccct
     4861 ccaatcctgg tgcccagcac gccatcgctg ctggtcgccg atgcctgtaa gacggaggca
     4921 cccgatcatg aaagacgcaa tccaccaggt cacggtcaat tttgccgacg gcgtcagccg
     4981 cagcttcgcc gtgcccgccg aggcgagcat cctcgatgcc gccatcgagg cggagatccc
     5041 gctgctctac cagtgccgct cgggcggctg ctccagctgc gtcgcccagc tcgccgaagg
     5101 cgacgcccgc acccgggccg gcgccagctc gtcgctgctg gccagcgagt acgccgccgg
     5161 ccagcgcctg ctgtgcctgt gccaggcgca gtcggactgc accttcaccg tgccctacgg
     5221 cagcgaggtc ggctccagcg tggccgttga ggtacacgcc ttcatcgact ccatcgaacg
     5281 catcgccgtc aacgtggtgc gcctgaccct ggaactggcc gacggcgact ggctggagtt
     5341 ccgtcccggc cagttcgtgc agatcgaggt gccgggcttc ggcgtgctgc gcagctactc
     5401 gccgtccagc accctggccg acctgcccaa gctgcagttg ctggtgcgcc tgctgccggg
     5461 cggggcgatg tccagctacc tggaggagaa ggcgcagatc gaggaggtgc tgaccctgcg
     5521 cggcccgtac ggggcgttct tcctgcgcga ggagcacaag cgcgcgccgc acatcttcgt
     5581 cgccggcggc accggcctgg cgccgatcct gtcgatgatc gacaccctgc gccagaacgg
     5641 cggcaagaag ccgccgatgc tgctcagctt cggctgcgcc accccggagg tgctgttcgg
     5701 tctcgacgac atcgagctgc gccagcagtg gctgccgagc ctggaggcgc gcatctgcgt
     5761 cgaccgcgag ccgaccggca acctgcacca cggcagcccg gtcagcgccc tgcgcgaagg
     5821 cgatgtggtc agtccggaca ccgttgccta cctgtgcggc ccgcagccga tgatcaatgc
     5881 cgccaccgcg cggctgatcg agctgggcgt cgccgccgaa aacatcttca gcgaacagtt
     5941 tgtggccagc cactgaggag ttccccatgc aaccgaccgt ttccgtgccg ttctcggccg
     6001 agcagatgat gtggtacgag caggcctgtg cgcgtatcga ccccaagcgc ctgcagaacc
     6061 tgctgttcga gctgaccaac atccacagcc cgaccggcgc ggccggcgcg gccagccagt
     6121 tcattgcccg ccacctggag tcggtcggtc tgtcggcgcg ctaccagccg atgagcccga
     6181 tcagcggcaa cgtgctggcc gagcgccgcg gcagcggcgg cggcgccagc gtgctgctgt
     6241 actcgccgat cgacacccac ctggaggagg gcgacgaggc ccaggagatc ctcaccggca
     6301 aggcgcccga ggtcgacatg cagatcacgg cgcggatgat cgacgactgg gtgttcggcc
     6361 tcggcgcctc caaccccaag ggcatggtcg ccaccctgac cgaagtggcc accgcgctga
     6421 tcgaggccga ggtgccgctg atcggcgacc tgctggtcgg catggccgac ggcggcatgc
     6481 cggtggacat cgccgggcgc gatcacgccg gcatgtccaa cggcgtgttc catctgctca
     6541 accgcggcgg ttcgccggac ttcgcggtga tcatgaagcc gtggaactgg gtctaccacg
     6601 aggaaccagg catggcctgg ttcaagctgc gcgtgcacgg caccctcggc tacgccggcg
     6661 tgccgcgcga cattccgggc ttccgcagct cggtggtgcc ggcggccacg gtgatccagg
     6721 agctggagcg ctggatcatc gactacgccg agcgcaacac ctccgggcag atcaagccgc
     6781 acggctggat cgccggggtg cgcggcggcg acccgcagcg cccggccttc ccctcggcgg
     6841 tcaccgagat cttcttcgac gtgcgggtca acccgcgcac cagcccggcg gcggtcaagg
     6901 cgcagttcgc cgccttcgtc cgcgagctgc aggcgcagcg tcccgaactg gacctggact
     6961 gggagatgta cggctcggtg gccggcggta gcaccgaccc gcagaactgg atcatccagt
     7021 cctgccagcg cggctgggag cacatcgagc accgtccgca cggccaggcc gacctgctcg
     7081 gcgggcagac cgacggcgcc gcgttgcgtc gcctgggcat tcccaccgcg cgcatcggct
     7141 ggccgtggcc ggcgcagg
//
LOCUS       DATCUN010000256         9281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_199466, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000256 DATCUN010000000
VERSION     DATCUN010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199466"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /gene="typA"
                     /locus_tag="VIO83_09830"
     CDS             complement(<1..1661)
                     /gene="typA"
                     /locus_tag="VIO83_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY8331612.1"
                     /translation="MIENLRNIAIIAHVDHGKTTLVDKLLRLSGTLDRKEAESERVMD
                     SNDQEKERGITILAKNTALKWNGYDINIVDTPGHADFGGEVERVMSMVDSVLLVVDAQ
                     DGPMPQTRFVTQKAFKAGLRPIVVVNKIDRPGARPDWVIDQIFDLFDNLGATDEQLDF
                     PIVYASALNGIAGMDHEKMDDDMDALFQAIVDHVPAPVVDASGPFQMQISQLDYNSFL
                     GVIGIGRIARGTIKANTPVTAIGADGKKRNGRILKIMGHSGLQRVEVQEAEAGDIVCV
                     SGMDELYISDTLCDQNNVEALPPLTVDQPTVSMTFQVNDSPFAGKEGKFVTSRNIKDR
                     LEKELLHNVALRVEPGDSPEKFKVSGRGELHLSVLIETMRREGFELAVGRPEVVIIEN
                     EAGDKQEPYENVTIDIEEQHQGPVMEQMGLRKGDLSNMVPDGKGRVRLEYTIPARGLI
                     GFRNNFLTMTSGSGILTSTFSHYGPIKAGEVTNRQNGVLVSMATGTALTYSLETLQAR
                     GKLFLAPGDEIYEGQLCGINSRDNDLVLNPTKGKKLDNMRASGKD"
     gene            complement(1803..3257)
                     /gene="thiI"
                     /locus_tag="VIO83_09835"
     CDS             complement(1803..3257)
                     /gene="thiI"
                     /locus_tag="VIO83_09835"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HEY8331613.1"
                     /translation="MKVIVKPFAEITIKSRPVRRQFVRQLAKNIRTVLRDLDPELVVQ
                     GEWDNLELETRQREPKLLAEMLERLRNTPGIAHCLEVHDYPLGDFDDIFDKCRLHFGA
                     QLAGKVFAVRCKRSGKHPFSSVDVATHVGSRLRRECGAAGIDLKHPEVEVRFEVRYDR
                     LFIIHAQHDGIGGYPLGSIEQTLVLMSGGFDSTVASYQMMRRGLLTHFCFFNLGGRAH
                     ELGVMEVAHYLWEKFGRSHRVLFISVPFEEVVGEILQKVDNSHMGVVLKRMMLRAASR
                     LAEELHIDALVTGEAISQVSSQTLPNLSVINAVTDTLVLRPLIASHKQEIIDTAERIG
                     TAEFAKNMPEYCGVISVNPTTRAKRGRVEHEETHFDMAVLERALERVRKVTIDKVIDE
                     LGEDLQIEEVREALPGQIVLDIRHPDAAEDQPLELPGIEVQALPFYAVNNRFKALDAN
                     RQYLLYCDKGVMSRLHAHHLLSEGHANVRVYRPA"
     gene            3582..4988
                     /gene="glnA"
                     /locus_tag="VIO83_09840"
     CDS             3582..4988
                     /gene="glnA"
                     /locus_tag="VIO83_09840"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY8331614.1"
                     /translation="MSKALQLIQEHDAKWIDLRFTDIKGRQHHITMPARDADEDFFDV
                     GKMFDGSSLDGWKGIEASDMILLPDDSTAVMDPFTEEPTLILVCDVIEPSTMQGYDRD
                     PRAIAKRAEEYLKSTGIGDTVFCGPEPEFFIFDSVKYKSDMSGSMFKIFSEQAAWSSD
                     ADTEGRQGNNGHRIAVKGGYLPTPPSDPDHEIRTAMCNALEEMGQVVEVHHHEVAGAQ
                     NEIGVKFNTLVAKADEVQTLKYCVHNVAEAYGKTVTFMPKPLYGDNGSGMHVHLSIAK
                     EGKNTFAGEGYAGLSDTALYFIGGIIKHGKALNALTNPSTNSYKRLVPGFEAPVMLAY
                     SARNRSASIRIPYVSSPKGRRIEARFPDPAANPYLAFAALLMAGLDGIQNKIHPGDAA
                     DKNLYDLPPEESKQIPQVCGSLKEALEALEADHDFLLKGGVFTKDFLEAFTELKSAEE
                     IKVRTFVHPLEYDLYYSC"
     gene            5174..5716
                     /locus_tag="VIO83_09845"
     CDS             5174..5716
                     /locus_tag="VIO83_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY8331615.1"
                     /translation="MRLTCTCLLLALALPASAEIYRYTDAHGNPVYTNQPPEGVSSES
                     VQLGPTNTVAAPPAKPTKPQAATELEGYSRLEISGMPDDDDTLRANAGNFTVDVSLEP
                     GLREGDLLQLLLDGQPLAAPGTQTSFRLGQVDRGTHSLQAVVLRDGEPLQTSASVSFT
                     VQRVHIGSPALPRPQPRPAN"
     gene            5721..6335
                     /locus_tag="VIO83_09850"
     CDS             5721..6335
                     /locus_tag="VIO83_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY8331616.1"
                     /translation="MRALLCLLLLTCLPAFAGVYTYLDADGNRVFTDQPRRANAERLE
                     LAPTNRADLPAPVASPPPALPAVLLPAYQLLRITLPEPDATIRDNSGGLLVTLESEPG
                     LLEEHSYRVLLDGKPYGAPARSPVFALDNIDRGTHQLAAEIIDGEGRIVERTPNQPFH
                     LKRISLAQKRQANPCQRDDYGVRPECPLADKPAEPRRLLGLPLP"
     gene            6650..7735
                     /gene="glnL"
                     /locus_tag="VIO83_09855"
     CDS             6650..7735
                     /gene="glnL"
                     /locus_tag="VIO83_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEY8331617.1"
                     /translation="MAYDSLQRLLLDNLTTAVILLNAELRLEYMNPAAEMLLGVSGQR
                     SQGMFIGELCHETPDGLSALRQAVANGHPFTKREAQLVSASGQHLAVDYAATPIPGLS
                     SFLLLLELHPRDRLLRISKEEAQLAKQETTRLLVRGLAHEIKNPLGGIRGAAQLLARE
                     LPEASLKDYTNVIIEEADRLRNLVDRMLGSNKLPQLVPTNIHEVLERVCSLVEAETQG
                     KLALERDYDPSIPELLADHEQLIQALLNIVRNAMQAILEQKHPAQPGRILLRTRTLRQ
                     FTIGHQRHRLVCRIEVIDNGPGIDPALQDTLFYPMVSGRADGTGLGLAIAQNILNQHQ
                     GLIECESQPGRTVFSLYLPLEQGAPTP"
     gene            7732..9162
                     /gene="ntrC"
                     /locus_tag="VIO83_09860"
     CDS             7732..9162
                     /gene="ntrC"
                     /locus_tag="VIO83_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEY8331618.1"
                     /translation="MNRPETVWIVDDDRSIRWVLEKALQQAGIANQSFDNADSLLNRL
                     SLEQPSVILSDIRMPGASGLELLARIRELYPRLPVIIMTAHSDLESAVASYQGGAFEY
                     LPKPFDVDEAVSLVKRATLHAREQQALATPDEQTATPEIIGEAAAMQEVFRAIGRLSH
                     SNITVLINGESGTGKELVAHALHRHSPRASAPFIALNMAAIPKDLMESELFGHEKGAF
                     TGAAAQRRGRFEQADGGTLFLDEIGDMPADTQTRLLRVLADGEFYRVGGHTPVKVNVR
                     IIAATHQNLETLVRAGKFREDLFHRLNVIRIHIPRLSDRREDIPALARHFLARAAQEL
                     AVEPKLLKPQTEEYLQNLPWPGNVRQLENTCRWITVMASGREVHVDDLPPELLQQQQE
                     APVGGSWEQSLRLWAEQALSRGQSDLLGSALPSFERVMIESALKHTAGRRRDAAQLLG
                     WGRNTLTRKIKELDMAVEGSDEDETL"
BASE COUNT         1676 a         3233 c         2887 g         1485 t
ORIGIN      
        1 tcgtccttgc cggaagcgcg catgttgtcg agcttcttgc ccttggtggg gttgagcacc
       61 aggtcgttgt cacggctgtt gatgccgcac agctggcctt cgtagatctc gtcgcccggc
      121 gccaggaaca gcttgccgcg cgcctgcagt gtttccagcg agtaggtcag tgcggtaccg
      181 gtagccatcg aaaccagcac gccgttctgg cggttggtca cttcgccggc cttgatcggg
      241 ccgtagtgac tgaaggtcga ggtcaggatg ccgctacccg aggtcatggt caggaagttg
      301 ttacggaagc cgatcaggcc gcgcgccggg atggtgtatt ccaggcgtac acggcccttg
      361 ccatccggaa ccatgttgct caggtcgccc ttacgcaggc ccatctgctc catcaccgga
      421 ccctggtgct gctcctcgat gtcgatggtg acgttctcgt acggctcctg cttgtcgccg
      481 gcctcgttct cgatgatcac cacttccggg cggcctacgg ccagctcgaa accttcacga
      541 cgcatggttt cgatcagtac cgaaaggtgc agctcgccac gaccggaaac cttgaacttc
      601 tcagggctgt cgcccggctc gacgcgcagg gcgacgttgt gcagcagctc cttctccaga
      661 cgatccttga tgttgcggct ggtgacgaac ttgccttcct tgccggcgaa cggcgagtcg
      721 ttgacctgga aggtcatgct cacggtcggc tggtcgacgg tcagcggcgg cagcgcttcg
      781 acattgttct ggtcgcacag ggtgtcggag atgtagagct cgtccatacc ggatacgcag
      841 acgatgtcgc ccgcttcggc ttcctgcact tcaacgcgct gcaggccgga gtggcccatg
      901 atcttgagga tacgaccgtt gcgcttcttg ccgtcggcac caatggcggt aaccggcgtg
      961 ttggccttga tggtgccgcg ggcgatgcgg ccgatgccga tcacgccgag gaagctgttg
     1021 tagtccagct gggaaatctg catctggaac gggccgcttg cgtcgacgac tggcgccggc
     1081 acgtggtcga cgatggcctg gaacagggca tccatgtcgt catccatctt ctcgtggtcc
     1141 ataccggcaa tgccgttcag ggcactggcg tagacgatcg ggaagtccag ctgctcgtcg
     1201 gtggcgccga ggttgtcgaa caggtcgaag atctggtcga taacccagtc aggacgcgcg
     1261 cccggacggt cgatcttgtt gaccacgacg atcggacgca ggccggcctt gaacgccttc
     1321 tgggtcacga aacgggtctg cggcatgggg ccgtcctggg cgtcgaccac cagcagcacg
     1381 gagtccacca tcgacatcac gcgctcgact tcgccaccga agtcggcgtg tccgggggtg
     1441 tcgacgatgt tgatgtcgta gccattccat ttcagcgcgg tgttcttcgc cagaatggta
     1501 atgccgcgct ctttttcctg gtcgttggag tccatcacgc gctcgctttc cgcttctttg
     1561 cggtcgaggg tgccggacag gcgcagcagc ttgtctacca gggtggtctt gccatggtcg
     1621 acgtgggcaa tgatggcgat gttgcggaga ttttcgatca cttgtgtgtt ttctcgatag
     1681 gtaacgcttg aactcggatg ccggagggga ttatcccggg ctgccggcga caacttgatt
     1741 aacgcgacgg ccagtggtcg ggagggcgat ggcgaatgct gccccccagc aggcccggct
     1801 gcttaggccg gacgataaac gcgcacattg gcgtgtccct cgctgagcaa gtggtgggca
     1861 tgcaggcggc tcatcacgcc cttgtcgcaa tacagcaggt actggcgatt ggcatccagc
     1921 gccttgaaac ggttgttgac cgcgtagaac ggcagggcct gcacctcgat accgggcagc
     1981 tccagcggct ggtcctcggc ggcgtcgggg tggcggatgt cgaggacgat ctgccccggc
     2041 agcgcctcgc gcacctcctc gatctgcagg tcctcgccga gctcgtcgat caccttgtcg
     2101 atggtcacct tgcgcacgcg ctcgagggcg cgctcgagga ccgccatgtc gaagtgggtt
     2161 tcctcgtgct cgacgcgccc gcgcttggcg cgggtggtcg ggttgaccga gatcaccccg
     2221 cagtactcgg gcatgttctt ggcgaactcg gcggtgccga tgcgctcggc ggtgtcgatg
     2281 atctcctgct tgtggctggc gatcagcgga cgcagcacca gggtgtcggt caccgcattg
     2341 atcaccgaca ggttgggcag ggtctggctg gacacctggg agatcgcctc gccggtgacc
     2401 agcgcgtcga tgtgcagctc ttcggcgagt cgcgacgcgg cacgcagcat catgcgcttg
     2461 agcaccacgc ccatgtggct gttgtcgacc ttctgcagga tctcgccgac cacttcctcg
     2521 aacggcacgc tgatgaacag cactcggtgc gagcggccga atttctccca caggtagtgg
     2581 gcgacttcca tcacgcccag ctcgtgggcg cggccaccga ggttgaagaa gcagaaatgg
     2641 gtcagcaggc cgcggcgcat catctggtag gaggccacgg tggagtcgaa gccgccggac
     2701 atcagcacca gggtctgctc gatggagccg agcgggtaac cgccgatgcc gtcatgctgg
     2761 gcatggatga tgaacagccg gtcgtagcgc acctcgaagc gcacctcgac ctcgggatgc
     2821 ttgaggtcga tgccggcggc gccgcactcg cggcgcaggc ggctgccgac atgggtggcc
     2881 acgtccaccg aggagaacgg gtgcttgccg gaacgcttgc agcgcaccgc gaacaccttg
     2941 ccggccagct gcgcaccgaa atgcagccgg cacttgtcga agatgtcgtc gaaatcgccc
     3001 agcgggtagt cgtgcacctc gaggcagtgg gcgatgcccg gcgtgttgcg cagccgctcg
     3061 agcatttccg ccagcagctt cggctcgcgc tgccgggttt ccagttcgag gttgtcccac
     3121 tcgccctgca ccaccagctc gggatcgagg tcccgcagca cggtgcgaat gttcttcgcc
     3181 agctgccgga cgaactgcct gcgtaccgga cggctcttga tggtgatttc ggcgaagggt
     3241 ttgacaatga ctttcatgat gggcgaacgg gaacggccaa gcgagaaaaa ggggacagat
     3301 tatagcggaa actgctcaac ctttgagcag aattccgcgc agcggataaa tgcaccaaaa
     3361 tgaatcacca aaacacccaa aagcacgcgt ttggtgcact aagctgacaa ctgcatcgag
     3421 tggtgcccgg aaaagccgca ttctgcgggc agcgtgcaca atcgcgcgca ctggcatgca
     3481 agttgcctat ataattcgga caattttccg gcgggcttcg ggcccgagaa aaaacgaaac
     3541 cgggcaccct catcgcccgt ctgcataccc tggaggaccg catgtcgaag gcgctgcaac
     3601 tgattcaaga acacgacgcg aagtggatcg acctccgctt caccgatatc aagggccgtc
     3661 agcaccacat caccatgccg gcacgcgatg ccgacgaaga cttcttcgat gtgggcaaga
     3721 tgttcgacgg ttcctccctc gacggctgga agggcatcga agcctccgac atgatcctgc
     3781 tgccggacga cagcaccgcc gtgatggacc cgttcaccga agagccgacc ctgatcctgg
     3841 tctgcgacgt gatcgaaccc tccaccatgc agggctatga ccgcgacccg cgcgccatcg
     3901 ccaagcgcgc cgaggaatac ctgaagtcca ccggcatcgg cgacaccgta ttctgcggtc
     3961 cggagccgga gttcttcatc ttcgattccg tgaagtacaa gtcggacatg tccggctcga
     4021 tgttcaagat cttctccgag caggctgctt ggagcagcga cgccgacacc gaaggccgcc
     4081 agggcaacaa cggtcaccgc atcgcggtca agggcggcta cctgccgacc ccgccgagcg
     4141 atccggacca cgaaatccgc accgccatgt gcaacgccct ggaagagatg ggccaggtcg
     4201 tcgaagtgca ccaccacgaa gtggctggcg cccagaacga aatcggcgtg aagttcaaca
     4261 ccctcgtcgc caaggctgac gaagtgcaga ccctgaagta ctgcgtgcac aacgtcgccg
     4321 aagcctacgg caagaccgtg accttcatgc cgaagccgct gtacggcgac aacggctcgg
     4381 gcatgcacgt gcacctgtcc atcgccaaag aaggcaagaa caccttcgcc ggcgaaggct
     4441 atgccggcct gtccgatacc gccctgtact tcatcggcgg catcatcaag cacggcaagg
     4501 cgctgaacgc cctgaccaac ccgtcgacca actcctacaa gcgtctggtc ccgggcttcg
     4561 aagctccggt catgctggcc tactcggccc gcaaccgttc cgcctcgatc cgcatcccgt
     4621 acgtgtccag cccgaagggc cgccgcatcg aagcgcgctt cccggacccg gctgccaacc
     4681 cgtacctggc cttcgccgcc ctgctgatgg ccggtctgga cggcatccag aacaagatcc
     4741 accccggcga cgccgccgac aagaacctgt acgacctgcc gccggaagag tccaagcaga
     4801 tcccgcaggt ctgcggcagc ctgaaggaag ccctggaagc cctggaagcc gaccatgact
     4861 tcctgctcaa gggtggcgta ttcaccaagg acttcctgga ggccttcacc gagctgaagt
     4921 cagccgagga aatcaaggtg cgcaccttcg tgcacccgct ggaatacgac ctgtactaca
     4981 gctgctaagt cagccgccga tcccgcatcg gcaatgtcac aaaggcctcc ttcgggaggc
     5041 ctttgtgctt tgtgcgacgg aaaaaccaca cgattgcgcc cggccgcaca ggccgcagcc
     5101 gcagcacttg ccagtgcgcc ccggcagtgc aaggcttgcc ccatcaccga ttgatgccga
     5161 aaggactccc cacatgcgcc tgacctgcac ctgcctgttg ctcgccctgg ccctgccggc
     5221 cagtgccgag atctatcgct ataccgacgc ccacggcaat ccggtctaca ccaaccagcc
     5281 gcccgagggc gtgagcagcg agagcgtcca gctgggaccg accaatacgg tcgctgcgcc
     5341 accggccaag ccgaccaagc cccaggccgc caccgagctg gaaggctaca gccgcctcga
     5401 gatcagcggg atgccggatg acgacgacac cctgcgcgcc aatgccggca acttcaccgt
     5461 cgacgtcagc ctggagccgg gcctgcgcga gggcgacctg ctgcagctgc tgctggacgg
     5521 ccagcccctg gcggccccgg gcacccagac cagcttccgc ctcggccagg tcgaccgcgg
     5581 cacgcacagc ctgcaggccg tggtcctgcg cgacggcgag ccgctgcaaa ccagcgccag
     5641 cgtcagcttc accgtgcagc gcgtgcacat cggcagcccg gccctgcccc gcccgcaacc
     5701 acgaccggcc aactgacccc atgcgcgctc tgctctgcct tctcctgctg acctgcctgc
     5761 cggccttcgc cggcgtgtac acctacctcg acgccgacgg caaccgggtg ttcaccgacc
     5821 agccgcgccg cgccaacgcc gagcgcctgg aactggcgcc gaccaaccgc gcggacctgc
     5881 ccgccccggt cgccagcccg ccgccggccc tgccggccgt gctgctgccg gcctaccagc
     5941 tgctgcgcat caccctgccg gagccggatg ccaccatccg cgacaacagc ggcggcctgc
     6001 tggtcaccct ggaaagcgaa ccgggcctgc tggaagagca cagctaccgc gtgctgctcg
     6061 acggcaagcc ctacggcgcc ccggcgcgca gtccggtctt tgcgctggac aacatcgacc
     6121 gcggcaccca ccagctggcc gcggagatca tcgatggcga aggccgcatc gtcgagcgca
     6181 cgccgaacca gcccttccac ctcaagcgca tctcgctggc gcagaagcgc caggccaacc
     6241 cctgccagag ggacgactac ggcgtacgcc cggagtgccc gctggccgac aagccggccg
     6301 aaccgcgccg cctgcttggc ctgcccctgc cctgagcacg acgcaccaat taagtgcgcg
     6361 cctgtgcccg gcgacctata ctgaaccacc gacagcgggc ggcgcgtaac gcccggcagg
     6421 gtctcccgcc gccgctgccg ccccgccgca gtcgccgaag cgctccgctg gcgcagcgct
     6481 gcaggccttg tcctaaaggc cttcggccag gccgacaggt cccccaggca agcgcaggca
     6541 cggcgagcgg cgtgcacaaa aaagcggcag tcgccggcct atctgtgcac cacggagtgc
     6601 tggcgcgctt cttgcaaaag ccatgcatcc acaggagcgc atgcttgcca tggcctacga
     6661 ctctctgcag cgcctgctgc tcgacaatct caccaccgcg gtcattctgc tcaatgccga
     6721 actgcgcctg gaatacatga acccggccgc cgagatgctg ctcggcgtca gcggccagcg
     6781 cagccagggc atgttcatcg gcgaactgtg ccatgagacg cccgacggcc tctccgccct
     6841 gcgccaggcg gtggccaacg gccacccgtt caccaagcgc gaggcgcagc tggtgtcggc
     6901 cagtggccag cacctcgcgg tggactacgc cgccacgccg attcccggcc tgagcagctt
     6961 cctgctgctg ctcgagctgc acccgcgcga ccgcctgctg cgcatcagca aggaggaagc
     7021 gcagctggcc aagcaggaga ccacccgcct gctggtgcgc ggcctggccc acgagatcaa
     7081 gaatccgctg ggcggcatcc gcggtgccgc ccagctgctg gcccgcgagc tgccggaggc
     7141 gagcctcaag gactacacca acgtgatcat cgaggaggcc gaccgcctgc gtaacctggt
     7201 ggaccgcatg ctcggctcca acaagctgcc gcaactggtg ccgaccaaca tccacgaggt
     7261 cctcgaacgc gtgtgcagcc tggtcgaggc ggaaacccag ggcaagctgg ccctcgagcg
     7321 cgactacgac ccgagcatcc ccgagctgct ggccgaccat gaacagctga tccaggccct
     7381 gctcaacatc gtgcgcaatg ccatgcaggc gatcctcgag cagaagcatc cggcgcagcc
     7441 cggacgcatc ctgctgcgca cccgcaccct gcgccagttc accatcggcc accagcgcca
     7501 ccgcctggtg tgccgcatcg aggtgatcga caacggcccg ggcatcgatc cggcgctgca
     7561 ggacaccctc ttctatccca tggtcagcgg ccgtgccgac ggcaccggcc tgggcctggc
     7621 catcgcccag aacatcctca accaacacca gggtctgatc gagtgcgaga gccagcccgg
     7681 acgcacggtt ttctcgctgt atctgccgct tgaacaagga gcaccaaccc catgaaccga
     7741 ccggaaactg tctggatcgt tgacgatgac cgctcgatcc gctgggtgct ggagaaagcc
     7801 ttgcaacagg cgggcatcgc caaccagagc ttcgacaacg ccgacagcct gctcaaccgc
     7861 ctgagcctcg agcagcccag cgtgatcctc tccgacatcc gcatgccggg agccagcggc
     7921 ctcgagctgc tggcgcgcat ccgcgagctg tacccgcgcc tgccggtgat catcatgacc
     7981 gcgcattccg acctggaaag cgcggtggcc tcctaccagg gcggcgcctt cgagtacctg
     8041 cccaagccgt tcgacgtcga cgaggcggtg tcgctggtca agcgcgccac cctgcacgcc
     8101 cgcgagcagc aggcgctggc caccccggac gagcagaccg ccaccccgga gatcatcggc
     8161 gaggcggcgg cgatgcagga ggtgttccgc gccatcggcc gcctcagcca ctccaacatc
     8221 accgtgctga tcaacggcga gtccggcacc ggcaaggagc tggtggcgca cgccctgcac
     8281 cgccacagcc cgcgcgccag cgcgccgttc atcgccctga acatggcggc gatccccaag
     8341 gacctgatgg agtcggaact gttcggccac gagaagggcg ccttcaccgg cgccgccgcg
     8401 cagcgccgcg gccgcttcga gcaggccgac ggcggcaccc tgttcctcga cgagatcggc
     8461 gacatgccgg ccgacaccca gacccgcctg ctgcgcgtgc tggccgacgg cgagttctac
     8521 cgggtcggcg ggcacacccc ggtcaaggtc aacgtgcgca tcatcgccgc aacccaccag
     8581 aacctggaaa ccctggtgcg cgccggcaag ttccgcgaag acctgttcca ccgcctcaac
     8641 gtcatccgca tccacatccc gcgcctgtcc gaccgccgcg aggacatccc ggcgctggcc
     8701 cgccacttcc tcgcccgcgc cgcccaggaa ctggcggtcg agcccaagct gctcaagccg
     8761 cagaccgagg agtacctgca gaacctgccg tggccgggca acgtgcgcca gctggagaac
     8821 acctgccgct ggatcacggt gatggcctcg gggcgcgagg tgcatgtcga cgacctgccg
     8881 ccggagctgc tgcagcagca acaggaagcc ccggtcggcg gcagctggga gcagagcctg
     8941 cgcctgtggg ccgaacaggc cctgagtcgc ggccagagcg acctgctcgg cagcgccctg
     9001 ccgtccttcg agcgggtgat gatcgagagc gcgctcaagc acaccgctgg ccgccgccgc
     9061 gacgccgccc agctgctcgg ctggggtcgc aacaccctga cccgcaagat caaggagctg
     9121 gacatggcgg tggaaggcag cgacgaggac gaaaccctgt aagccccgcc cagtcccggc
     9181 acgcgccagg tgtgccggga ctgggcgggc ggcacgatca ggccgcctta cccggcccgt
     9241 ccgacccgct cagcgcccgc cctgctcctg caggtcgtcg c
//
LOCUS       DATCUN010000257         4595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_256430, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000257 DATCUN010000000
VERSION     DATCUN010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256430"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VIO83_09865"
     CDS             <1..311
                     /locus_tag="VIO83_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014595381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein"
                     /protein_id="HEY8331619.1"
                     /translation="GQALGRAFRDLQAHQVALLAASRAAVKGILAQLAPEQLTLRFER
                     ERKPLLATAGSRWRAYRRLHETLAQDDDWSERLFARDFARTYEEQVRLIATLDTDLQG
                     "
     gene            317..811
                     /gene="tssJ"
                     /locus_tag="VIO83_09870"
     CDS             317..811
                     /gene="tssJ"
                     /locus_tag="VIO83_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HEY8331620.1"
                     /translation="MPRLFPLAVLAALLGLGGCSALSPYSHLTKLDLSLAGSDQLNPD
                     LNGRPSPIVLRLFELKHPVAFENADFFSLYQRPKEALSPDLVTLEELELRPGETRELK
                     LSVQADSRYVGVLAAYRDLPEAQWRFVIPLEAAARNRSALSLDERGIRDLAAADAPKN
                     QDED"
     gene            815..2146
                     /gene="tssK"
                     /locus_tag="VIO83_09875"
     CDS             815..2146
                     /gene="tssK"
                     /locus_tag="VIO83_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEY8331621.1"
                     /translation="MSQHKVVWQEGMLLRPQHFQQNDRYYEQQLKTRTQKLGHYAWGF
                     FSLEIDRQFLNMGKLVVSQASGILPDGSLFELDGGREPLAVDVPPNTGNTPVYLALPL
                     VTGNHIESRRAEQKDVLARYTACDEEVADSNAGDGGSSQVSTARPDFRLLLGEQRSDQ
                     AYVRLKLCDILDTTPDGVIALDAEFIPTFIDFQASSYLLSCLKEVISMLGHRGDILAE
                     RIRATGKVGGAEVGDFLMLQLINRHEPVLRHYLGLEQVHPEQIYRELLGLLGELATFS
                     SESKRPRLDSRYQHSDQGASFRKLMDAIRQVLSMVLEQHAIELALQQRQYGIQVAPLH
                     DHQLLGSAAFVLAASAQCDSEELRSRLPAHLKVGAVERIRQLVNLHLPGIKVKPLPVA
                     PRQIPFHAGKTYFALELNAEELAQLERSGGFAFHVSGEFAELELKFWAIRN"
     gene            2152..3018
                     /gene="icmH"
                     /locus_tag="VIO83_09880"
     CDS             2152..3018
                     /gene="icmH"
                     /locus_tag="VIO83_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HEY8331622.1"
                     /translation="MTRAMDYAKDDRTVILNRHGEAAAHSPLTDFASPPHYEQLEERM
                     VYAARLRPAESFNLGLNPLVAAAAVLLSEAVRLKHSFESEDLHALHQHLSGELKLFEH
                     RALHDGAESSQVMAARYVLCTVLDEAVVTTPWGNESEWSQMSLLSSFHNETFGGEKLF
                     QLLERLSRNPVKHLPMLELMYLCLALGFEGKYRVQPRGMLELEAIRDSLYRQIRQLRG
                     DVPREVSPHWQGLKDTRRRLVRIVPWWLVALFTLACLLVIYGGFAWVLGEQRDAVLQP
                     YQSLDGHAAGQP"
     gene            3037..>4595
                     /gene="tssM"
                     /locus_tag="VIO83_09885"
     CDS             3037..>4595
                     /gene="tssM"
                     /locus_tag="VIO83_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016703143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HEY8331623.1"
                     /translation="MKDLFAKLGAFFRQTWVWSLCLLLVLALLVWFAGPLLAVAEHKF
                     WASPTSRLLSIAALCLLWGLFVVFAGWRANRRQQAEEADQAAQERLRREDQVSEEQGI
                     LRQRYQDALRTLKHSSLYRGHTERWRSELPWYLLLGPQGSGKTSLLDFSGLDFPLNRG
                     DAQRLTRDVAGTRHADWYFAEHAVLIDTAGRYLTQPDAQVDGSAWDCLLGLLRKRRTR
                     PLNGVLVNIPLERLQGGNELELEALARQVRQRLQELHQRLGVEVPVYLVLSKADQLLG
                     FDEFFDQLSREDSEQVLGASFRKEQDSTAASVVRQEFEELLRRLNSQVIQRMHQERDT
                     QRRGRILDFPHQLGQIGERLCLFVELAFAGNRYQRASQLRGFYLTSAPRLDSPLDPLT
                     AGIGRNLGLANSALPTLRSGRTRFIHNLLSRVIFPEADLASLDQQEVRRIDWGQRALY
                     AGALACLALFGLAWANSYSANHGQLEQLRENAQQLGRERQGIAAQDDALRTLAALDRS
                     HAATQVFAPAAA"
BASE COUNT          736 a         1731 c         1479 g          649 t
ORIGIN      
        1 ccggccaggc gctcggccgt gccttccgcg acctgcaggc gcaccaggtg gcgctgctcg
       61 ccgccagccg cgccgcggtc aagggcatcc tcgcccagct cgccccggaa cagctgaccc
      121 tgcgcttcga gcgcgaacgc aagccgctgc tggccaccgc cggcagccgc tggcgcgcct
      181 accgccgcct gcacgaaacg ctggcgcagg acgacgactg gagcgaacgc ctgttcgccc
      241 gcgacttcgc caggacctac gaagaacagg tgcgcctgat cgccaccctc gataccgacc
      301 tgcaaggatg atccgcatgc ctcgcctgtt tcccctcgcc gtgctggccg cgctgctcgg
      361 cctgggcggc tgctcggcgc tgtcgccgta ctcgcacctg accaagctgg acctcagcct
      421 ggccggtagc gaccagctca accccgacct caacggccgg ccctcgccga tcgtgctgcg
      481 cctgttcgag ctcaagcatc cggtggcgtt cgagaacgcc gacttcttca gtctctacca
      541 gcggcccaag gaggccctgt cgcccgacct ggtgaccctc gaggagctcg agctgcgccc
      601 cggcgagacc cgcgagctga agctgtcggt gcaggcggac agccgctacg tcggcgtgct
      661 cgccgcctac cgcgacctgc cggaagccca atggcgcttc gtcatcccgc tcgaggccgc
      721 cgcgcgcaac cgcagcgcgc tgagcctcga cgagcgcggc attcgcgatc tcgccgccgc
      781 tgacgcgccg aaaaaccagg acgaggactg agccatgagc cagcacaagg tggtctggca
      841 ggaaggcatg ctgctgcgcc cgcagcactt ccagcaaaac gaccgctact acgagcagca
      901 gctgaagacc cgcacccaga aactgggcca ctacgcctgg ggcttcttca gcctagagat
      961 cgaccgccag ttcctcaaca tgggcaagct ggtggtcagc caggccagcg gtatcctccc
     1021 cgacggcagc ctgttcgagc tcgacggcgg ccgcgagccg ctggccgtcg acgtgccgcc
     1081 gaacaccggc aacaccccgg tgtacctggc gctgccgctg gtcaccggca accacatcga
     1141 gagccgccgc gcggagcaga aggacgtgct ggcgcgctac accgcctgcg acgaggaggt
     1201 cgccgactcc aacgccggcg acggcggcag cagccaggtc agcaccgcac ggccggactt
     1261 ccgcctgctg ctcggcgagc agcgcagcga ccaggcctac gtccgcctca agctgtgcga
     1321 catcctcgac accaccccgg acggggtgat cgccctggac gccgaattca tcccgacctt
     1381 catcgacttc caggcttcca gctacctgct gtcctgcctc aaggaagtga tcagcatgct
     1441 cggccaccgc ggcgacatcc tcgccgagcg catccgcgcc accggcaagg tcggcggcgc
     1501 cgaggtcggc gacttcctga tgctgcagct gatcaaccgc cacgagccgg tgctgcgcca
     1561 ctacctcggc ctcgagcagg tgcacccgga gcagatctac cgcgagctgc tcggcctgct
     1621 cggcgagctg gcgaccttct ccagcgagag caagcgcccg cgcctggaca gccgctatca
     1681 gcacagcgac cagggcgcca gcttccgcaa gctgatggat gcgatccgcc aggtgctgtc
     1741 gatggtcctc gagcagcacg ccatcgaact ggccctgcag cagcgccagt acggtatcca
     1801 ggtggcgccg ctgcacgacc accagctgct cggctcggcc gccttcgtgc tcgccgccag
     1861 cgcccagtgc gactcggaag agctgcgctc gcgcctgccg gcgcacctca aggtcggcgc
     1921 ggtggagcgc atccgccagc tggtcaacct gcacctgccg gggatcaagg tgaagccgct
     1981 gccggtggcg ccgcggcaga tccccttcca cgccggcaag acctacttcg ccctcgagct
     2041 gaacgccgag gaactggccc agctggagcg ctccggcggc ttcgccttcc acgtctccgg
     2101 cgagttcgcc gagcttgaac tgaaattctg ggcgatcagg aactgaccga catgacccgc
     2161 gcaatggact atgccaagga cgaccgcacg gtcatcctca accgccacgg cgaagccgcg
     2221 gcccacagcc cgctgaccga cttcgccagc ccgccgcact acgaacagct ggaggagcgc
     2281 atggtctacg ccgcgcgcct gcgcccggcg gagagcttca acctcggcct caacccgctg
     2341 gtcgccgccg ccgccgtgct gctctccgag gccgtgcgcc tcaagcacag cttcgagagc
     2401 gaggacctgc acgcgctgca ccagcacctg agcggcgaac tcaagctgtt cgagcaccgc
     2461 gccctgcacg acggcgccga gagcagccag gtgatggcgg cgcgctacgt gctgtgcacc
     2521 gtgctcgacg aggcggtggt gaccaccccc tggggcaacg agagcgagtg gtcgcagatg
     2581 agcctgctgt cgagcttcca caacgagacc ttcggcggcg agaagctctt ccagctgctc
     2641 gaacgcctgt cgcgcaaccc ggtcaagcac ctgccgatgc tcgagctgat gtacctgtgc
     2701 ctggcgctag gcttcgaggg caagtaccgc gtgcagccgc gcggcatgct cgagctggag
     2761 gcgatccgcg acagcctgta ccggcagatc cgccagctgc gcggcgacgt gccgcgcgag
     2821 gtgtcgccgc actggcaggg cctcaaggac acccgccgcc gcttggtgcg catcgtgccc
     2881 tggtggctgg tggcgctgtt caccctggcc tgcctgctgg tgatctatgg cggcttcgcc
     2941 tgggtgctgg gcgaacagcg cgacgccgtt ctgcaaccgt atcaatcgct cgacggccac
     3001 gccgccgggc agccgtaatt cagggatgaa tgcacgatga aagacttgtt cgccaaactc
     3061 ggcgcctttt tccgccagac ctgggtgtgg agcctgtgcc tgctgctggt gctggccctg
     3121 ctggtgtggt tcgccggccc gctgctggcg gtcgccgaac acaagttctg ggcgtcgccg
     3181 accagccgcc tgctgagcat cgccgcgctg tgcctgctgt gggggctgtt cgtggtcttc
     3241 gccggctggc gcgccaaccg ccggcagcag gccgaggagg cggaccaggc cgcccaggag
     3301 cgcctgcgcc gcgaggacca ggtcagcgag gagcagggca tcctgcgcca gcgctaccag
     3361 gacgccctgc gcaccctcaa gcactccagc ctgtaccgtg gccacaccga gcggtggcgc
     3421 agcgagctgc cctggtacct gctgctcggc ccgcagggca gcggcaagac cagcctgctg
     3481 gacttctccg gcctggactt cccgctcaac cgcggcgacg cccagcgcct gacccgcgac
     3541 gtcgccggca cccggcatgc cgactggtac ttcgccgagc atgcggtgct gatcgacacc
     3601 gccggccgct acctgaccca gcccgacgcc caggtcgacg gcagtgcctg ggactgcctg
     3661 cttggcctgc tgcgcaagcg ccgcacccgt ccgctcaacg gcgtgctggt caacatcccg
     3721 ctggagcggc tgcagggcgg caacgaactg gagctcgaag ccctggcccg ccaggtccgc
     3781 caacgcctgc aggagctgca ccagcgcctc ggcgtcgagg tgccggtcta cctggtgctg
     3841 agcaaggccg accagctgct cggcttcgac gagttcttcg accagctgtc gcgcgaggac
     3901 agcgagcagg tgcttggcgc cagcttccgc aaggagcagg acagcaccgc tgcctccgtg
     3961 gtgcgccagg aattcgagga gctgctgcgc cgcctgaaca gccaggtgat ccagcgcatg
     4021 caccaggagc gcgacaccca gcgccgcgga cggatcctcg acttcccgca tcaactcggc
     4081 cagatcggcg agcgcctgtg cctgttcgtc gaactggcct tcgccggcaa ccgctaccag
     4141 cgcgccagcc agctgcgcgg cttctacctg accagcgcgc cgcggctgga cagcccgctc
     4201 gacccgctga ccgccggcat cggccgcaac ctgggcctgg ccaacagcgc cctgccgacc
     4261 ctgcgcagcg ggcgcacgcg cttcatccac aacctgctga gccgggtgat cttccccgag
     4321 gcggacctcg ccagcctcga ccagcaggaa gtgcggcgca tcgactgggg ccagcgcgcg
     4381 ctgtacgccg gcgccctggc ctgcctggcg ctgttcggcc tggcctgggc gaacagctac
     4441 tcggccaacc acggccaact cgaacagctg cgcgagaatg cccagcagct gggccgcgag
     4501 cgccagggca tcgccgccca ggacgacgcc ctgcgcaccc tcgcggcgct ggatcgcagc
     4561 catgccgcga cccaggtgtt cgcgcccgcc gccgc
//
LOCUS       DATCUN010000258         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_199531, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000258 DATCUN010000000
VERSION     DATCUN010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199531"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..557)
                     /gene="fliS"
                     /locus_tag="VIO83_09890"
     CDS             complement(144..557)
                     /gene="fliS"
                     /locus_tag="VIO83_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEY8331624.1"
                     /translation="MYGSNRQFAEQYRKVGVSTSVSEADPHKLVALLFAGACQRIRLA
                     QASLLQGDQARKGKAIGEACAIVGHLNGSLDHEAGGEIAANLSSLYEYLMHRLTQANL
                     HNDDAALTEALELLGEINSAWDAIPADQRGTPARA"
     gene            complement(702..2024)
                     /gene="fliD"
                     /locus_tag="VIO83_09895"
     CDS             complement(702..2024)
                     /gene="fliD"
                     /locus_tag="VIO83_09895"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEY8331625.1"
                     /translation="MATPTVSGSGLDIPTLVSSLVTAARTPTQNRINAAGTASTAKLS
                     AISQVKSIMTSLQGSLSAVASSADTAAYKATVQNEAGFTASTSSAAAAGSYSVTVESL
                     ATPQKQATATAYAQDHSFGSGSLTIAYGQDGTEIQVQIGQDATLADIATAVNKASGGK
                     GVVASVITADDGQHLVLGAVGPGTANALTVTATGADLQALAGALSTTVQATDAVVTVD
                     GFKRTLASNTVQDLVPGVTLTLTKADPDTSYTLTVAPDNSALKTNLNGFASAYNAAMT
                     LLKSSSAYDPDTQTASALTGDSLVRGLQQQLRGMVSANLTDLKALGIALDKDGVMSFD
                     AASLDKAIAADPEAAARTLGKDGSYSAAMTRLLDSNLNATGGTLVLRTNALNKQIKDL
                     EGQLDDLDERMAKLSDLYTRQFTAMETMIVQLQGSASSLDGLLSMSSD"
     gene            complement(2329..>2448)
                     /locus_tag="VIO83_09900"
     CDS             complement(2329..>2448)
                     /locus_tag="VIO83_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY8331626.1"
                     /translation="ADYAKETAELTRTQILQQAGTAMLAQAKSVPQNVLSLLQ"
BASE COUNT          333 a          787 c          876 g          452 t
ORIGIN      
        1 gtccacgctc tggcagtacg ccttcaggtg caggtcgtga tcggccagca tctgcggcat
       61 gcgctcgagc tgatcgtgcg ccagggcact gtgcagcgcc gcgatctcgg cctgcaggct
      121 ctggacgctg tgggtggact gggtcatgcc cgtgccgggg tgccgcgctg gtcggcggga
      181 attgcatccc aggccgagtt gatttcgccc agcaattcca gcgcttcggt cagcgcagca
      241 tcatcgttgt gcaggttggc ctgggtcagc cggtgcatga ggtattcata caacgaggac
      301 aaattggccg cgatctcgcc accggcctca tgatcgagcg atccgttgag atggccgaca
      361 atcgcgcagg cctcgccgat ggccttgccc ttgcgggcct ggtcaccttg cagaaggctg
      421 gcctgggcca gccggatgcg ctggcaggca ccggcaaaca gcaacgcgac cagcttgtgc
      481 ggatcggctt cgctgacact ggtggacacg ccgaccttgc ggtactgctc ggcaaactgg
      541 cggttggaac cgtacatggg atcattcctc aagatggccg cagctggtgc ccgcacagcg
      601 gcggcgacgg atcgcggccg ggctgggcga gcgggtccga tggtggacca gacctttatc
      661 ggcatggtgc cggcacgact tgagcatcac gccggccggc ctcagtcgga ggacatcgac
      721 aacaacccgt ccagcgaact ggcgctgccc tgcaactgga cgatcatcgt ctccatggcg
      781 gtgaactgcc tggtgtacag gtccgagagc ttggccatgc gctcgtccag gtcgtccagt
      841 tgtccctcca ggtccttgat ctgcttgttg agcgcgttgg tccgcagcac cagggtgccg
      901 ccggtggcgt tgagattgct gtccagcagc ctggtcatgg ccgcgctgta gctgccatcc
      961 ttgccgagcg tcctggcagc cgcctccggg tccgcggcga tcgccttgtc caacgaggcg
     1021 gcatcgaaac tcatcacccc gtccttgtcc agggcgatgc ccagcgcctt gaggtcggtc
     1081 aggttggcgc tgaccatgcc gcgcagctgc tgctgcaggc cgcgcaccag cgagtcgccg
     1141 gtcagggccg aggcggtctg ggtgtcgggg tcataggcac tgctgctctt gagcagggtc
     1201 atggctgcgt tgtaggcgct cgcaaagccg ttcaggttgg tcttcagcgc gctgttgtcc
     1261 ggggccacgg tcagggtgta gctggtgtcg gggtcggcct tggtcagggt gagcgtgacc
     1321 ccgggcacca ggtcctgcac ggtgttgctg gcgagcgtgc gtttgaagcc atcgaccgtg
     1381 accaccgcat cggtggcctg gaccgtggtg ctcagcgccc cggccagggc ctgcaggtcc
     1441 gccccggtgg cggtgaccgt cagcgcattg gccgtacccg gcccgaccgc gcccagcacc
     1501 aggtgctggc cgtcgtcggc ggtgatcacg ctggcaacca cccccttgcc gccggaggcc
     1561 ttgttcaccg ccgtggcgat gtcggccagg gtggcgtcct ggccgatctg cacctggatt
     1621 tcggtcccgt cctggccata ggcgatggtc aggctgccgc tgccgaaact gtggtcctgg
     1681 gcgtaggcgg tggccgtggc ctgtttctgt ggggtggcca ggctctcgac cgtgacgctg
     1741 tagctgccgg ccgccgccgc cgacgaggtg ctggcggtga agccggcctc gttctgcacg
     1801 gtcgccttgt aggcggcggt atcggcgctg ctggccaccg ccgacagcga gccctgcagg
     1861 ctggtcatga tgctcttgac ctggctgatt gccgacaact tggcggtgga ggcggtgccg
     1921 gccgcgttga tccggttctg ggtcggcgtg cgcgccgcgg tgaccaggct ggagaccagg
     1981 gtgggaatgt ccaggccgga accggaaacg gtgggagtgg ccatgcgtct gttcctcgtg
     2041 ggccgcgcgc gggtggatga tggcgtcctg caatggatat cggcgccgcc gccgcgatct
     2101 tgagcggccg cctgcgcacc atcggtcagt gacaggcaag tcctgtgccg ttaggcgagg
     2161 cggtgcctgg cccgcccgcc ccgcgcgcga ccgagccgtt gggcagctcg cttgcggccg
     2221 tgggccgctg ccacgcagcg gccgctcgcc ccggctccgg tgcaacggcc caaaagcaaa
     2281 acccctccga agaggggctc tgcctgaagc gcgaaggctg gccgcggatt actgcagcag
     2341 gctcagcacg ttctgcggga ccgacttggc ctgggccagc atcgccgtac cggcctgctg
     2401 caggatctgg gtgcgggtca gctcggcggt ttccttggcg tagtcggc
//
LOCUS       DATCUN010000259         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_236874, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000259 DATCUN010000000
VERSION     DATCUN010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236874"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VIO83_09905"
     CDS             complement(<1..1029)
                     /locus_tag="VIO83_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEY8331627.1"
                     /translation="MFRLLFTLFVCGACSVLQAAPQLAPARLQALAGDPFWLSLGHYE
                     RGKLGGWRSYVDDDEFFLAADGAEDPAAELAATLEALYRDASLGDRHPQCIYPARTRW
                     LREQLALDDLPALRCPEFAAWYADIAPHSTVLVFPAAYLNSPSSMFGHTLLRIDQADI
                     DRQGSPLLSYALNFGAYIEGMDNSILYAWKGLMGGYPGLFALMPYREKIGEYSRLENR
                     DLWEYRLNLTAEETARLLEHVWELRQIQFDYFFFDENCSYRLLELLEIARPGLELTAG
                     FPLTAIPADTVRAVKDAGLVARIDYRPSRERELLTRAAPLDPLEQGWVQSLAADSGAL
                     QDAGYQALP"
     gene            complement(1118..1606)
                     /locus_tag="VIO83_09910"
     CDS             complement(1118..1606)
                     /locus_tag="VIO83_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HEY8331628.1"
                     /translation="MKRILLGSLLAAATFNAFAEAPGGPGCGWGNMLFEGQRGLPSHL
                     VASTTNGTSGNATFGMTSGTNGCSTKGALRYDGKSMLVLSSIMTELSEDMAKGEGEAL
                     TTYAVVLGVQPQDRAHFAAVTHQHFGEIFSSADVTAEQVHAATLAVLKQDARLAQYAD
                     KA"
     gene            complement(1739..>2555)
                     /locus_tag="VIO83_09915"
     CDS             complement(1739..>2555)
                     /locus_tag="VIO83_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016567271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HEY8331629.1"
                     /translation="GAALPRGADREARDFGQFGLALHYHYEPLATDFGAYALNYHSRL
                     ALLGLSGDRYVLAYPEDIRLYGLSFATTLAGGSVWRGELSHRPDAPLQPSIADGLASG
                     AAQAAYRRHAVSQLQTGVEHRFDQLMGASRLTLVGELAWIHVGELDGRYGRDPLFGPG
                     PQTGGCARYCEDDGFTTRDAWGYRLQAVWEYPRVFAGIDLKPRLSWAHDVDGHSPAPE
                     STMVEGRQALSLGLDADYLDTYTASLAYTNFFGGRYSTQGDRDFLALELGLRF"
BASE COUNT          394 a          821 c          959 g          381 t
ORIGIN      
        1 cggcagcgcc tggtagccgg cgtcctgcag cgccccgctg tccgccgcca ggctctgcac
       61 ccacccctgc tccagcgggt cgagcggcgc cgcgcgggtc agcagctcgc gctcgcgcga
      121 ggggcgatag tcgatgcgcg cgaccagccc ggcgtccttg accgcgcgca cggtgtcggc
      181 cgggatggcg gtgagcggga agcctgcagt cagctccagc cccggccggg cgatctccag
      241 cagttcgagc aggcggtagg agcagttctc gtcgaagaag aagtagtcga actggatctg
      301 ccgcagctcc cacacgtgct cgagcaggcg cgcggtttcc tcggccgtca ggttcaggcg
      361 gtattcccac aggtcgcggt tttccaggcg gctgtactca ccgatcttct cgcggtaggg
      421 catcagcgcg aacagcccgg gatagccgcc catcaggccc ttccaggcat acaggatgct
      481 gttgtccatg ccctcgatgt aggcgccgaa gttcagtgca tagctgagca gcgggctgcc
      541 ctggcggtcg atgtcggcct ggtcgatacg cagcagggtg tggccgaaca tcgacgaggg
      601 gctgttgagg taggcggcgg ggaacaccag cacggtactg tgcggggcga tgtcggcgta
      661 ccaggcggcg aactcggggc agcgcagcgc cggcaggtcg tccagcgcca gctgctcgcg
      721 cagccagcgg gtgcgcgccg ggtagatgca ctgcgggtgg cggtcgccga ggctggcgtc
      781 gcgatacagc gcctcgagcg tggccgccag ttcggcggcc gggtcctcgg cgccgtcggc
      841 agcgaggaag aattcgtcgt cgtcgacgta gctgcgccag ccccccagct tgccgcgctc
      901 gtagtggccg agggaaagcc agaacgggtc gccggccagg gcctgcaggc gggcgggagc
      961 gagttgcggg gcggcctgca gcacgctgca ggcgccgcac acgaagaggg tgaacagaag
     1021 tcggaacatc ggcagccagc ggcgagggaa acacgcaagt gcccgccgcg gcgggcacct
     1081 ggagaaggcg ggcgccccgg agggtgcccg cggcggatca ggccttgtcg gcgtactgcg
     1141 ccaggcgtgc gtcctgcttc agcacggcca gggtggcggc gtgcacctgc tcggcggtga
     1201 cgtcggcgga gctgaagatt tcgccgaagt gctggtgggt gacggcggcg aagtgcgcgc
     1261 ggtcctgcgg ctgcacgccg agtaccacgg cgtaggtggt cagggcttcg ccctcgccct
     1321 tggccatgtc ttcggacagc tcggtcatga tgctgctcag caccagcatc gacttgccgt
     1381 cgtagcgcag ggcgcccttg gtggagcagc cgttggtgcc cgaggtcatg ccgaaggtgg
     1441 cgttgccgga cgtgccgttg gtggtcgagg cgaccaggtg cgaaggcagg ccgcgctggc
     1501 cttcgaacag catgttgccc cagccgcaac ccggaccgcc cggggcttcg gcgaaggcgt
     1561 tgaaggtggc ggcggcgagc agggaaccca gcagaatccg tttcatggtt gatctcttct
     1621 tcaatgtgat gacgcaggca ggcgaccttg ccggccctgc cgcgcagctt tttgcaatat
     1681 cccggcaatg tcaaggcgat gacccgggca gcgcctgcga ctgtgcgccg cgtcgaggtc
     1741 agaagcgcag ccccagctcg agggccagga aatcgcgatc gccctgggtg ctgtagcggc
     1801 cgccgaagaa gttggtgtac gccaggctgg ccgtgtaggt gtccaggtag tcggcatcca
     1861 ggccgaggct gagcgcctgg cgcccttcga ccatggtgga ctccggcgcc ggcgaatggc
     1921 catcgacatc gtgggcccag ctcaggcgcg gcttgaggtc gatgccggcg aacacccgcg
     1981 gatactccca gacagcctgc agccggtagc cccaggcatc gcgggtggtg aaaccgtcgt
     2041 cctcgcaata gcgcgcacag ccgccggtct gcggccctgg tccgaacagc gggtcgcggc
     2101 catagcgccc gtccagctcg ccaacgtgga tccaagccaa ctcgccgacc agggtcaggc
     2161 ggctggcccc catcagctga tcgaaacggt gttcgacacc ggtctgcagc tggctgacgg
     2221 catgccggcg ataggcggct tgggccgcgc cactggccag gccgtcggcg atgctcggct
     2281 gcagtggcgc atcgggccga tggctgagtt cgccgcgcca gacgctgccg ccggccaggg
     2341 tggtggcgaa gctcaggccg tacaggcgga tgtcctcggg gtaggcgagg acgtagcggt
     2401 cgccggacag gccgagcagc gccaggcgac tgtggtagtt gagcgcatag gcgccgaagt
     2461 cggtggccag cggctcgtag tgatagtgca gggccaggcc gaactggccg aagtcgcggg
     2521 cttcgcgatc ggcgccgcgc ggcagcgccg cgccg
//
LOCUS       DATCUN010000260         7540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_11470, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000260 DATCUN010000000
VERSION     DATCUN010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11470"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VIO83_09920"
     CDS             <1..630
                     /locus_tag="VIO83_09920"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8331630.1"
                     /translation="FGYGLSSKPSDGNFDFETLMDCLIKLFDHLKLDKVTLVGNSMGG
                     AMSIRFAQDFPQRVNKLVVMGPGGIGPMERYLAMPAIQMLKALGQEEGGFSKDKLRRF
                     LEFMAYSKDDVTDQLVNERYEVAVTQPPRVFQTLSIGDLRPRLNILKNLPTMVLWGRD
                     DRACPVESGMEIMKECDNARLIVFSQCGHWVQYEQAKLFNKLCLDFLAE"
     gene            complement(674..1162)
                     /gene="tadA"
                     /locus_tag="VIO83_09925"
     CDS             complement(674..1162)
                     /gene="tadA"
                     /locus_tag="VIO83_09925"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY8331631.1"
                     /translation="MSSTKIPAEDTAGRDRHWMARAIELALAAQAQGEVPVGAVLVAD
                     EAVLGEGFNRPIGACDPTAHAEIVALRAAAAQAGNYRLPGSTLYVTLEPCAMCAGAIV
                     QARVQRVVFGARDPRAGAAGSVFQVFGEARLNHRTAVTEGVLGEHCGALLSDFFRRRR
                     AG"
     gene            complement(1137..1475)
                     /locus_tag="VIO83_09930"
     CDS             complement(1137..1475)
                     /locus_tag="VIO83_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331632.1"
                     /translation="MASRLLAPVVLALGLVACTQETQNEIGRAVQNWTGTNGVLEVYS
                     GGQLVKRFLDIDKLTTASGTKDDRPRPYRFGYGVLDQNLNGQRDPGEKKVYFEISDYS
                     TPYVFYENPR"
     gene            complement(1657..2208)
                     /locus_tag="VIO83_09935"
     CDS             complement(1657..2208)
                     /locus_tag="VIO83_09935"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF007479.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEY8331633.1"
                     /translation="MNAPTQIQTPANPRHVDRDTLFEVERFLIREARMLDEERFREWW
                     QTQLTEDIHYVLPVREIRMRADKKPVGSNPGAFVFNDNYPMLDMRIRRLETGLVWMED
                     PQNYIRRFVSNIDAAWADVDGEVDAWSNFIVYRNRRQRDETVVWGTKQCRLRKVAGQW
                     KLAGRTILVDQRVVLDKNLYFFI"
     gene            complement(2210..3532)
                     /locus_tag="VIO83_09940"
     CDS             complement(2210..3532)
                     /locus_tag="VIO83_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY8331634.1"
                     /translation="MKDVTKLILEDGAVQDRRIYWDQDIYEQELEQIFARAWQFLAHE
                     GQIPNAGDFITTRMGEDNVIVARQPDGTIKAFINSCPHRGNVVCHADTGNAKAFTCAY
                     HGWAFGLNGELATVPLEHDAYYDAIERPRYAMHPVAKVESYKGLVFGTFDARAPSLRD
                     YLGDMAWYMDTFLDVPGGIELLGPPMKSILNCNWKVPVENFIADGYHVGWAHAAALKA
                     IESPLSPLAGNQAYDVNTGLQVATRGGHGFGVIWDAAAAIHPEGGAFRDWLAKRTPIM
                     RERLGEWRSRFYNGHWDASIFPNCSFLYGTNTWKLWLPMGPHNIEVWTWTLVEKEMPA
                     ELKQMMVATNMMTFGTAGMLETDDGENMEQCTGSNRGWVTRRGKLYSGMGQVREGVHA
                     ELPGIIGKGIVCETSNRGFYRRWAELMSGRSWEEISREPHYAPNREVA"
     gene            3738..4460
                     /locus_tag="VIO83_09945"
     CDS             3738..4460
                     /locus_tag="VIO83_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEY8331635.1"
                     /translation="MSDTIAVIGATRGTGLAAMRQLIDAGRPAAAVVRSIEKARGLFG
                     EKATLVYGDVTRPETLAQAIDPDWAGIVFTVDITGGIAGRGMFASREKIRAVMYGGLV
                     NTVEACKQRGFKGRLVLLSTIGLTEPSTAMAILNRVKPGLRDHSLDKGEYLKKSGLDW
                     TIVRAGILNDKPGDAHALTITAQDWPLQMRYQIARADLARVMLACVTHPAVGKRELSV
                     FWGEAGHDSDAELDAKLAAAAG"
     gene            4477..5262
                     /locus_tag="VIO83_09950"
     CDS             4477..5262
                     /locus_tag="VIO83_09950"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEY8331636.1"
                     /translation="MLARWRDWLAGHRRASAPQLAQDWPAAFARVPVLAHWPPADRER
                     LQRLALDFLAGKVLEPVGQAAIDRPQALAIALQAALPVLNLGLDWYRGWHAVVLYPDE
                     FRSRFEEVDEAGVVHQVEDWRSGESWSAGPLILSLAEVAAGGQGHGHNVVVHECAHKL
                     DQLDGHSNGRPPLHPDMDPAAWSSAFAAAYRHLGRRALRHLRTVLDPYGAQSPAEFFA
                     VASEMFFDDPLGLQRGYPAVYAQLALFYRQDPAAHWPAPAEPP"
     gene            5259..5843
                     /locus_tag="VIO83_09955"
     CDS             5259..5843
                     /locus_tag="VIO83_09955"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY8331637.1"
                     /translation="MSLPEQILLLVLVTDPFGNLPLVLAALGRLDAAAYRRAVLRESL
                     FAFAVLLVLGWGGAGLLRTFGITEPALHIGGGVILFLLSLRMIYGGPQGAFQDGYAAD
                     PLLVPIAVPAIAGPAAIATVILLRTQQQVPLGQLALGLLGALLVTLVTFLPGRRIARW
                     LGPRGLNALEKLTGLLLNLVSVNMMLEGVRHFLA"
     gene            5923..6690
                     /gene="fnr"
                     /locus_tag="VIO83_09960"
     CDS             5923..6690
                     /gene="fnr"
                     /locus_tag="VIO83_09960"
                     /inference="COORDINATES: protein motif:HMM:NF008365.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEY8331638.1"
                     /translation="MTADPPPANVVTLSGLKAACSTCNLHELCLPHGMSNADVAELER
                     IVSRTRPFSRGRTLFRADEPFRAIYVPRSGAVKTYILGADGSEQIIGFHLPGEVIGLD
                     GMVEHRHQLTAQALELTGVCEVPFDLLEHAAARVPALQHQLMRLMSREIAGKEQQLLT
                     LGDHSPERRLALLLLDLSQRYARRGFSATEFNLPMSRQDIAAYLRLAAETVSRAFGKL
                     QRDGLVDVNGRLVCITNSDALAAFSGQLPAGDQSRVS"
     gene            complement(6834..>7540)
                     /locus_tag="VIO83_09965"
     CDS             complement(6834..>7540)
                     /locus_tag="VIO83_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY8331639.1"
                     /translation="EGLAGRSIARLLAALRAYYRHLLDAGCLLADPTAGIAAPKSPRR
                     LPATLDADHLARLLDFAGEDPLARRDRALFELLYSSGLRLAEVVALDRADLDLADASA
                     RVTGKGNKTRIVPVGSRARDALGDWLSLRATLAADCPALFVNRRGGRLSPRTVQQRLR
                     HWADRQGIDRRVYPHLLRHSCASHLLESSGDLRAVQELLGHASLATTQIYTHVDFQYL
                     ARVYDAAHPRARRKAP"
BASE COUNT         1166 a         2514 c         2586 g         1274 t
ORIGIN      
        1 ttcggctacg gcctgtcgtc gaagcccagc gatggcaatt tcgatttcga aaccctgatg
       61 gactgcctga tcaagctgtt cgatcacctg aagctggaca aggtgaccct ggtcggcaac
      121 tcgatgggcg gcgccatgtc catccgcttc gcgcaggatt tcccgcagcg ggtgaacaag
      181 ctggtggtga tgggccccgg tggcatcggg ccgatggagc gttacctggc catgccggcc
      241 atccagatgc tcaaggcgct cggtcaggag gagggcggct tcagcaagga caagctgcgt
      301 cgcttcctgg aattcatggc ctactccaag gacgacgtca ccgatcagct ggtcaacgag
      361 cgctacgagg tggccgtcac ccagccgccg cgggtgttcc agacattgtc catcggcgac
      421 ctgcgcccgc gcctgaatat cctgaagaac ctgccgacca tggtgctgtg gggccgcgat
      481 gatcgcgcct gtccggtgga gtcaggcatg gaaatcatga aagagtgcga caacgcgcgc
      541 ttgatcgtgt tctcgcagtg cggacactgg gtgcagtacg aacaggccaa gctgttcaac
      601 aagctttgcc tggattttct ggcggaatga ttcgtgggcc gcacccgccc ggctggcggg
      661 cgggtgcgct ttctcagccg gcgcggcggc ggcgaaaaaa gtcgctcagc agcgcgccgc
      721 agtgctcgcc cagcacgccc tcggtcaccg ccgtccggtg attcaggcgc gcctcgccaa
      781 atacctgaaa taccgatccc gccgctccgg cgcgcggatc gcgggctccg aacaccaccc
      841 gctgcacgcg cgcctgcacg atcgccccgg cgcacatggc gcacggttcg agcgtcacat
      901 acagcgtgct gccgggcagg cggtaattgc cggcctgcgc ggccgccgcg cgcagggcca
      961 cgatttcggc atgggcggtc gggtcgcagg cgccgatcgg ccggttgaag ccttccccga
     1021 gcaccgcctc gtcggccacc agcaccgcac cgaccggcac ctcgccctgc gcctgcgcgg
     1081 caagcgccag ttcgatcgcg cgcgccatcc agtggcggtc gcgcccggca gtgtcctcag
     1141 cggggatttt cgtagaagac atagggcgtc gagtagtcgc tgatctcgaa atacaccttc
     1201 ttctcgcccg gatcgcgctg accgttgagg ttctggtcca gcacgccgta gccgaaccgg
     1261 tacggccgcg gccgatcgtc cttggtgccg gaggcggtgg tcagcttgtc gatgtcgaga
     1321 aaacgtttca ccaactggcc gcccgagtaa acctccagca cgccgttggt gccggtccag
     1381 ttctgcacgg cgcggccgat ttcgttctgg gtttcctgcg tacaggcgac aagacccagg
     1441 gcgagcacga ccggcgccaa tagccgcgat gccattgctg gactccttga aaagcgccga
     1501 tctggcgcgg gtgcgaagta tgccggcaga cgctgcgcgt gagcgcgtcg ccgatgccgc
     1561 aagtcaggct ggaatatggg gggtcaagtc gacccgcgca agaagagatg ccgttgccgg
     1621 ccgccgatac ccccggcggc cgacaacggc cacagactag atgaagaaat acaggttttt
     1681 gtccagcacc acgcgctggt cgaccaggat ggtgcgaccg gccagcttcc attggccggc
     1741 caccttgcgc aggcggcact gcttggtgcc ccagaccacg gtttcgtcgc gctggcggcg
     1801 gttgcgatag acgatgaagt tcgaccaggc gtcgacttcg ccgtccacgt ccgcccaggc
     1861 cgcatcgatg ttgctgacaa agcggcggat gtagttctgc gggtcctcca tccacaccag
     1921 gccggtttcc aggcgccgga tgcgcatgtc gagcatcggg tagttgtcgt tgaagacgaa
     1981 cgcgccgggg ttgctgccga ccggcttctt gtccgcccgc atgcgaattt cgcgcaccgg
     2041 caggacgtag tggatgtcct cggtcagctg cgtctgccac cactcgcgaa agcgctcctc
     2101 gtcgagcatg cgcgcttcgc ggatcaggaa acgctcgacc tcgaacagcg tgtcgcggtc
     2161 cacgtggcgg gggttggccg gggtttggat ctgggtcggt gcgttcatgt caggccacct
     2221 cgcggttggg ggcgtagtgc ggttcgcggc tgatttcttc ccaactgcgg ccgctcatca
     2281 gctcggccca gcggcggtag aagccgcggt tggaggtctc gcacacgatg cccttgccga
     2341 tgatgcccgg cagctcggcg tgtacgccct cgcgcacctg gcccatgccc gagtacagct
     2401 tgccgcggcg cgtcacccag ccacgattgc tgccggtgca ttgctccatg ttctcgccgt
     2461 cgtcggtctc gagcatgccg gcggtgccga aggtcatcat gttggtggcg accatcatct
     2521 gcttgagctc ggccggcatt tccttctcga ccagggtcca ggtccacacc tcgatgttgt
     2581 gcgggcccat cggcagccac agcttccagg tgttggtgcc gtacaggaac gagcagttgg
     2641 ggaagatgct ggcatcccag tggccgttat agaaacggct tcgccattcg ccaagccgct
     2701 cgcgcatgat cggcgtgcgc ttggcgagcc aatcccggaa cgcgccgccc tcgggatgga
     2761 tggccgccgc ggcatcccag atgaccccga agccatgccc gccgcgggtc gcaacctgca
     2821 ggccggtgtt gacgtcgtag gcctggttgc cggccagcgg cgacagcggg gactcgatgg
     2881 ccttcagcgc cgcggcgtgg gcccagccga cgtggtaacc gtcggcgatg aagttctcga
     2941 ccggcacctt ccagttgcag ttcaggatcg acttcatcgg cggaccgagc agctcgatgc
     3001 cgcctggaac gtccaggaag gtgtccatgt accaggccat gtcgcccagg tagtcgcgca
     3061 gcgacggcgc cctggcgtcg aaggtgccga acaccaggcc cttgtagctt tccaccttgg
     3121 ccaccgggtg catggcgtag cgcgggcgct cgatggcgtc gtagtaggcg tcgtgctcca
     3181 gcggcacggt ggccagttcg ccattgaggc cgaaggccca gccgtgatag gcgcaggtga
     3241 aggccttggc gttgccagta tcggcgtggc acaccacgtt gccgcggtgc gggcaggagt
     3301 tgatgaaggc cttgatcgtg ccgtccggct ggcgggcgac gatgacgttg tcctcgccca
     3361 tgcgcgtggt gatgaagtcg ccggcgttcg gaatctgccc ctcgtgggcc aggaactgcc
     3421 aggcgcgggc aaatatctgt tccagctcct gctcgtagat gtcttgatcc cagtagatgc
     3481 ggcggtcctg caccgcgccg tcttccagga tcagcttggt aacgtccttc acgcgaaacc
     3541 ctccttggtg tggggaagcc accctgcgcg atccgggcag ggcggcggca cgtaggcact
     3601 cctaggtgcg tacgccgcgc atattggaag cgcgcgccgg cagcctcttg accggtatca
     3661 aggcggccgc gttgcactgg ccagcgccgg ggcggcagtt agaatcggcc gcagtcacac
     3721 gaacagggga gacggacatg agcgatacga ttgccgtgat cggtgccacg cgcggcacgg
     3781 gcctggcggc catgcgccag ctgatcgacg ccggccgacc agccgccgcg gtggtgcgca
     3841 gcatcgagaa ggcgcgcggc ctgttcggcg agaaggcgac gctggtctac ggtgacgtca
     3901 cgcgcccgga gacgctggcg caggccatcg atccggactg ggccggcatc gtgttcacgg
     3961 tggacatcac cggcggcatc gccggacgcg gcatgttcgc cagccgcgaa aaaatccgcg
     4021 ccgtcatgta cggcggcctg gtcaacaccg tggaagcgtg caagcagcgc ggcttcaagg
     4081 gccggctggt gctgctgtcg acgattggtc tgaccgaacc ctccacagcc atggcgatcc
     4141 tgaaccgggt caagccgggt ctgcgcgacc actcgctgga caagggcgag tacctgaaaa
     4201 agagcggcct ggactggacc atcgtgcgcg ccggcatcct gaacgacaaa cccggcgatg
     4261 cccacgccct gaccatcacc gcccaggact ggccgctgca gatgcgctac cagatcgccc
     4321 gcgccgacct ggcgcgcgtg atgctggcct gcgtgaccca tccggccgtc ggaaagcgcg
     4381 agctgagcgt gttctggggc gaggccggcc acgacagcga cgccgagctc gacgccaaat
     4441 tggccgcggc ggccggctag attctggagc gcgcgcatgc tcgcgcgctg gcgcgattgg
     4501 ctggcgggac atcgcagggc atcggcaccg cagctcgcgc aggactggcc ggccgccttc
     4561 gcccgcgtgc cggtgctggc ccactggccg ccggccgatc gggaacggct gcaacggctt
     4621 gcgctcgact ttctggcggg caaggttctg gagccggtcg gccaggctgc catcgaccgc
     4681 ccgcaggcgc tggccatcgc cctgcaggcc gcgctgccgg tcctcaatct gggccttgac
     4741 tggtaccgcg gctggcacgc ggtggtgctg tacccggacg agtttcgcag ccgcttcgag
     4801 gaggtggacg aggccggcgt ggtgcaccag gtcgaggact ggcgcagcgg cgagtcctgg
     4861 agtgccgggc cgctgatcct gtcgctggcc gaggtggcag ccggcgggca gggccacggc
     4921 cacaacgtcg tcgttcacga atgcgcgcac aagctcgatc agctcgacgg ccacagcaac
     4981 ggtcgcccgc cactgcaccc ggacatggac ccggccgcct ggagcagcgc tttcgccgcc
     5041 gcgtaccgcc atcttggccg gcgtgcgctg cgccacctgc gcaccgtgct cgacccgtac
     5101 ggcgcccagt cgccggccga gttcttcgcc gtggcgagcg agatgttctt tgacgatccg
     5161 cttggcctgc agcgcggcta cccggccgtc tacgcgcaac tggccctgtt ctaccgccag
     5221 gatccggccg cgcactggcc ggcgccggcc gagccgccat gagcctgccg gaacagatac
     5281 tgctgctggt gctggtcacc gacccgttcg gcaacctgcc gctggtgctg gcggctctgg
     5341 ggcgactcga cgctgccgcc taccggcgcg ccgtactgcg cgagtcgctg tttgcgttcg
     5401 ccgtgctgct ggtgctcggc tggggcggtg cgggcctgct gcgcaccttc ggcatcaccg
     5461 agccggcgct gcacatcggc ggcggggtga ttttgttcct gctgtcgctg cgcatgattt
     5521 acggcggccc gcagggcgcc ttccaggacg gctacgcggc cgacccgttg ctggtgccga
     5581 tcgccgtgcc ggcgattgcc gggccggcgg ccatcgccac cgtcatcctg ctgcgcaccc
     5641 agcagcaggt gccgctgggc cagctcgcgc tgggcctgct cggagccttg ctggtcaccc
     5701 tggtcacgtt tctgccgggg cgacgcatcg cacggtggct tgggccgcgc ggcctcaacg
     5761 ccctggaaaa gttgaccggc ctgctgctga atctggtctc ggtgaacatg atgctggagg
     5821 gtgtgcggca ctttctggcc tgaggcgctg tgcaccacaa ccgaccatgg accctatttg
     5881 tgttaggttt cacgcacgtt tccccacatc acactggaac gcatgacagc cgaccccccc
     5941 ccggcaaacg tcgttaccct cagtggcctc aaggctgcct gcagtacctg caatctgcac
     6001 gagctgtgtc tgccgcacgg catgagcaat gccgatgtgg ccgagctgga gcgcatcgtg
     6061 tcccgcaccc gcccattcag tcggggcagg acgctgtttc gggccgacga gccattccgg
     6121 gccatctatg tgccgcgcag tggagcggtc aagacctaca tcctgggcgc agacggcagc
     6181 gagcagatca tcggctttca cctgcccggc gaggtgattg gcctggatgg catggtcgag
     6241 caccgccatc agctcaccgc tcaggccctg gagctgaccg gcgtgtgcga ggtgccgttc
     6301 gatctgctgg aacacgcggc ggcccgcgtg ccggccctgc agcaccagct gatgcgcctg
     6361 atgagccgcg agattgccgg caaggagcag cagttgctga ccctgggcga ccattcgccg
     6421 gagcgacgcc tggcgctgct gctgctcgat ctgtcgcagc gctacgcgcg gcgcggcttc
     6481 tcggcgaccg agttcaacct gccgatgtca cgccaggaca tcgcggccta tttgcgcctg
     6541 gcggccgaaa cggtaagccg tgccttcggc aagctgcagc gcgatgggct ggtggatgtc
     6601 aacggtcgtc tggtatgcat caccaacagc gatgcgctgg cggccttctc gggccagctg
     6661 ccggcaggcg accagagccg ggtttcctga cggcgtctgc gccgggggat atgttgtggg
     6721 agcccggctt gccgggcgat gcgcccttgc atattgccct tgaggattgc cggttcatcg
     6781 cgcagcgaag ctgcgctcta caggcgccat tcccaagggc atccggttcc cggtcagggg
     6841 gccttgcggc gtgcccgcgg gtgcgccgcg tcgtacacgc gcgccagata ctgaaaatcg
     6901 acgtgcgtgt aaatctgcgt ggtggcgagg ctggcgtggc ccagcagttc ctgcaccgcg
     6961 cgcaggtcgc cggatgattc cagcagatgg ctggcgcagg aatgacgcag caggtgcggg
     7021 tagacgcgcc ggtcgatgcc ctggcggtcg gcccagtgac gcaggcgctg ctggacggtt
     7081 cgcggcgaca gacgcccacc gcggcggttg acgaacagcg ccggacagtc tgcggccagg
     7141 gtcgcgcgca ggctgagcca gtcaccgagg gcgtcccgcg cccggctgcc gaccggcacg
     7201 atgcgcgtct tgttgccctt gccggtgacc cgcgcgctgg cgtcggcaag atcgagatcg
     7261 gcccggtcga gcgccaccac ctcggccagg cgcaggccgg acgagtacag cagctcgaac
     7321 agcgcccggt cgcgacgggc gagcggatcc tcgccggcaa agtcgagcag gcgcgccaga
     7381 tggtcggcgt ccagggtggc cggcaggcgg cgcgggcttt tgggcgccgc aatgccggcc
     7441 gtcggatcgg ccagcaggca gccagcatcg agcagatggc ggtaatacgc ccgcagcgcc
     7501 gccagcagcc gggcaatgct gcgcccggcc aggccctcgc
//
LOCUS       DATCUN010000261        51531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_49702, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000261 DATCUN010000000
VERSION     DATCUN010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 51531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51531
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49702"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /gene="rlmD"
                     /locus_tag="VIO83_09970"
     CDS             <1..1110
                     /gene="rlmD"
                     /locus_tag="VIO83_09970"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003175384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEY8331640.1"
                     /translation="RREAPCALAGRCGGCILQHLSHAEQVALKQRALAEQLHRLAALA
                     PQEWAPPLLGPEFGYRRRARIAVRYSPKSGQLEVGFRAAASQAIVELDQCAVLVQALQ
                     PLFAALPGVLRRLRKPQAIGHVELFHGTASALLVRHTQGLAEEDRQLLRAFCSEQGAQ
                     LWWQGEGEPQADDPAQPLGYRLEACDLTLAWRPGDFVQVNAPVNELMLAQALEWLAPQ
                     ADERVLDLFCGLGNFALPLAQRAAEVVGVEGVQGMVERAAANATANGLGNAHFFRADL
                     SEPLAQAPWAKGGFAAVLLDPPRDGAAAVVGQLRSLGAQRVLYVSCNPATLARDAAIL
                     AQQGYRLKRAGILDMFPQTAHVEAMALFEAGAERP"
     gene            1258..3504
                     /gene="relA"
                     /locus_tag="VIO83_09975"
     CDS             1258..3504
                     /gene="relA"
                     /locus_tag="VIO83_09975"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017527554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HEY8331641.1"
                     /translation="MVQVRAEQPINTDGSINLEAWLDHILDVDPALDRDSLRKACEVA
                     REAEQQAIAVQHIWAEGMSSFRTGLEIAEILADLKLDQESLIAAVLYRGVREGKVQLE
                     VVEKDFGVPVATLIEGVLRMAAISTSLSPRQSLVLGTQAQVENLRKMLVAMIDDVRVA
                     LIKLAERTCAIRAVKNADDDKRYRVAREVFDIYAPLAHRLGIGHIKWELEDLSFRYLQ
                     PEQYKQIAKLLHERRLDREQYINQVMQQLRDELTATGIEADISGRAKHIYSIWRKMQR
                     KGLEFSQIYDVRAVRVLVPEVRDCYTALGIVHTLWRHIPKEFDDYIANPKENGYRSLH
                     TAVIGPEGKVLEVQIRTQAMHEEAELGVCAHWLYKGTDVNSGSSHYEEKIAWLRQVLE
                     WHEELGDIGGLAEQLRVDIEQDRVYVFTPDGHAIDLPTGATPLDFAYRVHTEIGHNCR
                     GAKVNGRIVPLSYSLQTGEQVEIITGKHGAPSRDWLNPNLGYVTTSRARAKIVHWFKL
                     QARDQNVIAGKSYLERELARLALTAVDAERLAEKCNLKSTEDMYAALGAGDLRLAQVV
                     NAAQQLVEPERGNEMLDLLSRRGPSGRSGGRDEIQILGVGNLLTQMAGCCQPVPGEPI
                     VGYITQGRGVSIHRQDCPMALQQTEREPERMIQVSWGQAPAKTYPVDIQIRAYDRSGL
                     LRDVSQMLLNEKINVLAVNTQSNKSDNTANMSLTIEIPSMAALGRLLARISQLPNIIE
                     ARRSRSGA"
     gene            3512..4348
                     /gene="mazG"
                     /locus_tag="VIO83_09980"
     CDS             3512..4348
                     /gene="mazG"
                     /locus_tag="VIO83_09980"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEY8331642.1"
                     /translation="MYRLDDLLHLMARLRDPQSGCPWDLAQDYASIVPHTLEEAYEVA
                     DAIERGDFAHLPGELGDLLFQVVYYSQLGREEGRFDFAAVVDGITRKLVRRHPHVFPD
                     GDLYGAADLPQLDEAAIKRRWEEIKAEERAEKAAAPEQLSLLDDIPQALPALSRAAKL
                     QKRAAQVGFDWPAPLPVLDKLQEELDEVLEAMAEGDQEHIAEELGDLLFVVVNLARHL
                     KVEPEAALRAANGKFERRFQFIERAVRASGRALGDCSLDELDALWGEAKRAERQQTGC
                     GG"
     gene            complement(4400..5332)
                     /locus_tag="VIO83_09985"
     CDS             complement(4400..5332)
                     /locus_tag="VIO83_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEY8331643.1"
                     /translation="MPATLIKVSLLGAFVASNLYVHLRGQARLPLLRQMVNHSSLFSP
                     YNSLMYLFSRTPSQPYIERRDFPELDLLRDNWQTIREEALALFDEGYIRAALHDNEAG
                     FGSFFKRGWKRFYLTWYDEQPLPSAAKLCPKTLELLGQIPSVKGAMFTMLPAGSHLNP
                     HRDPFGGSLRYHLGLATPNSDACRIFVDGQPRAWRDGEDMMFDETYVHWVKNETDADR
                     LILFCDVERPLPEPLAGFNRWISRTLGRATAPQNVEGERVGAINRAYSGFKRVGNAVG
                     GVIRQFKRKNPKAYRVLRPIAAVAIAVLLYRWIV"
     gene            5561..6103
                     /locus_tag="VIO83_09990"
     CDS             5561..6103
                     /locus_tag="VIO83_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HEY8331644.1"
                     /translation="MSMSLRDQLLKAGLVNEKQAKQATKQKQKQQRLEHKGQAEKDDS
                     QRQAALQAQAEKTARDLELNRQQQEKVQKKALAAQVKQLIESSRLPRLEGDDYYNFVD
                     DKKVKRITVNPMLRDKLSRGTLAIVRLEGKYELIPREAALKVQEREPQRIIVLNVASE
                     VPDDEDDPYKDYVVPDDLMW"
     gene            complement(6312..7022)
                     /locus_tag="VIO83_09995"
     CDS             complement(6312..7022)
                     /locus_tag="VIO83_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY8331645.1"
                     /translation="MRRALTLALVLFGLPASALALEPFTAQYTADWQQVPVSGTAERQ
                     LDKGADGSWTLQFKASMLVSGLTEVSRFRYDNQRFVPLSYRFERNGLGKSKKVAHDFD
                     WKTKLVTGNDRDTPVKLVLKSGLLDKSTYQLALQEDVAAGKKSMTYQVLDGDEIETYD
                     FRVLGEEQVQTEAGQIDALKVERVRDPTKSNRQTVLWFAKDWGHLLVRLHQVEKDGKE
                     YQIMLKQGAVNGRNVQGK"
     gene            complement(7061..7723)
                     /gene="purN"
                     /locus_tag="VIO83_10000"
     CDS             complement(7061..7723)
                     /gene="purN"
                     /locus_tag="VIO83_10000"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY8331646.1"
                     /translation="MAQPCNVVVLISGSGSNLQALIDSLGEDNPARICAVISNRADAY
                     GLTRAHNAGIATAVLDHKAFAGREAFDAALIETIDAFQPDLVVLAGFMRILSAGFVHH
                     YHGRLLNIHPSLLPRHKGLHTHQRALEAGDGEHGCSVHFVTEELDGGPLVVQGVVPVQ
                     PGDSPEALAKRVQIGEHHIYPLAVRWFAEGRLRLANHGAELDGVPLPASGQQIRDLPL
                     PR"
     gene            complement(7723..8781)
                     /gene="purM"
                     /locus_tag="VIO83_10005"
     CDS             complement(7723..8781)
                     /gene="purM"
                     /locus_tag="VIO83_10005"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY8331647.1"
                     /translation="MSKQPSLSYKDAGVDIDAGEALVERIKGVAKRTARPEVMGGLGG
                     FGALCEIPAGYKQPVLVSGTDGVGTKLRLALNLNKHDSIGQDLVAMCVNDLVVCGAEP
                     LFFLDYYATGKLNVDVAATVVTGIGAGCELAGCSLVGGETAEMPGMYEGEDYDLAGFC
                     VGVVEKSEIIDGSKVAAGDALIALPSSGPHSNGYSLIRKIIEVAGADIEQIQLDGKAL
                     TELLMAPTRIYVKPLLKLIKDTGAVKAMAHITGGGLLDNIPRVLPEGAQAIIDVASWN
                     RPAVFDWLQQQGNVDEHEMHRVLNCGVGMVICVAQDQVEAVLANLRASGEQPWVIGSI
                     ASGAEGAERVVLNNLKAH"
     gene            8947..9990
                     /locus_tag="VIO83_10010"
     CDS             8947..9990
                     /locus_tag="VIO83_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HEY8331648.1"
                     /translation="MRLIARWFAFCLLFATLSATAAQVEGLYEVHEPVTSQDPAERAA
                     AMSRALQTLVVRLTGDRQAAQNPRLQPLLADPQQLVQQYVYESGTPVTLSVVFDPVLS
                     QRALREAGLTLQTAERASVLAWWLNEADGASSLVAEDQPVAATLRAAAQNRGVPLSLP
                     LGDLDEQLLATADTLAGSDAQALQEASARYAADALLAVVARGAGDSWQADWQLWVGSE
                     RVTGSSTAASQAALADAVLLEVGEKLASRFSKAGAAQSLTLEVQGSDLARYAALERLL
                     EPFAPRLLEVSSGGRLVYQVDASPDQLRSQLQLGRLHEVPAEAVDAQGAVPAANPPLQ
                     VPAETAKRLRFAW"
     gene            10039..11136
                     /locus_tag="VIO83_10015"
     CDS             10039..11136
                     /locus_tag="VIO83_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8331649.1"
                     /translation="MMLNDSRSWLGLAALVLLGWLVHQLQPVLMPFLVALLLAYLGDP
                     LVDRLEARGLSRTSGVVVVFILFSLILLALLLVLVPLLGKQLVRLYELAPEMLDWLQG
                     TALPWVQKKLGLRYAFWRFDRIKTLLAEHLGQATDIAGILLAQVTSSSLALLAWLGNL
                     LLIPVVSFYLLRDWDVMMGHLRNLLPRKREEWVVRLAGECHEVLGAFLRGQLLVMLAL
                     ALVYAGGLMLVGLELGLLIGLLAGLASIVPYMGFVVGIVAALVAVLFQFGLDWYPLLG
                     VVVVFGIGQLLEGMWLTPLLVGDRIGLHPVAVIFAILAGGQLFGFTGVLLALPVAAVI
                     MVLLRHMHEFYKLSRLYGESGGDSQESSESP"
     gene            11133..11837
                     /gene="hda"
                     /locus_tag="VIO83_10020"
     CDS             11133..11837
                     /gene="hda"
                     /locus_tag="VIO83_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEY8331650.1"
                     /translation="MKPIQLPLGVRLRDDATFANYYPGANAAALGYVERMCETDVGWA
                     ENLIYLWGAEGVGRSHLLQAACLRMEQFGGRAVYLPLDTVAMYGTQLLDNLEQCDLVC
                     IDEVDLVAGHPTWEEALFHLFNRLRDAGRRLLLSASAPPREVPIKLPDLKSRLTLALV
                     FQLHGLSDEDKLRALQLRASRRGLHLTDEVGRFILTRGSRSMNSLFDLLEQLDNASLQ
                     AQRKLTIPFLKETLGW"
     gene            complement(11961..12368)
                     /locus_tag="VIO83_10025"
     CDS             complement(11961..12368)
                     /locus_tag="VIO83_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEY8331651.1"
                     /translation="MAKKNKPLPALDWLEPRVRLARWLSLALFVALVVLLGVWYLLLE
                     DLHGARPWVILGVHLVPLAILAPGMLTGNARVHAWTCYVLNLYFIQGVVAAFQPDRAL
                     FGWLEAGLSLTLFCSALLYTRWRFQYDRKLAGE"
     gene            complement(12370..12966)
                     /gene="wrbA"
                     /locus_tag="VIO83_10030"
     CDS             complement(12370..12966)
                     /gene="wrbA"
                     /locus_tag="VIO83_10030"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEY8331652.1"
                     /translation="MSAPYILVLYYSRHGATAEMARQIARGVEQGGLEARLRTVPAVS
                     AECAAVAPEIPEQGALYASLDDLRHCAGLALGSPTRFGNMAAPLKHFLDGTSSLWMTG
                     ELVGKPAGVFTSTASLHGGQESTQLSMLLPLLHHGMLVCGLPYSEQALLDTRGGGTPY
                     GASHHAGNDGKRPLDEHETALCRALGQRLAHTAQKLGN"
     gene            complement(12963..13316)
                     /gene="arsC"
                     /locus_tag="VIO83_10035"
     CDS             complement(12963..13316)
                     /gene="arsC"
                     /locus_tag="VIO83_10035"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244313.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEY8331653.1"
                     /translation="MTDMILYHNPRCSKSRGALELLEARGLQPRVMHYLETPPSAAEL
                     RELLGKLGYGARQLLRSGEELYKELNLADPALDDEALIAAMALHPRLIERPILVVGAR
                     AVVGRPPERVLELLA"
     gene            13427..14665
                     /locus_tag="VIO83_10040"
     CDS             13427..14665
                     /locus_tag="VIO83_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEY8331654.1"
                     /translation="MNRHLYHLWCFVERLIRRFFADRGLQSAAALTYTTLFAVVPMLT
                     VTFVMLAAVPAFRDLGEPIQAFIFRNFVPSAGEQVQQYLGGFISQARQLTWIGVAVLV
                     VSALLTLSTIEGAFNHIWRVRRARRGVSSFLLYWAILSLGPLLLGAGFAASTYVTSLE
                     LLSGPHALPGAATLLGLMPLLTGIAAFTLLYAAVPNTQVSGRHALLGGCFSALLFEAA
                     KALFALYVSLFPSYQLIYGAFAAVPLFLLWIYLGWLIVLLGAELVCHLGDPGSWRGVA
                     RPPLLNLLGLLGQLFARQQGGRSLSRAALARDGWALSEEEWLELIGFLERERLVSATS
                     NGDWVLCRDLSQLSLAWLVERSPWRLPALAALPEHLPAGLDAPWYPRLRAAVQALEQA
                     RAEALGGSLAEWLGEARGRR"
     gene            complement(14677..15048)
                     /locus_tag="VIO83_10045"
     CDS             complement(14677..15048)
                     /locus_tag="VIO83_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014603632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331655.1"
                     /translation="MLPNQDRLLHALTAQQLAAARPRSTALDGRLVELEVAAWLHLPG
                     LSDLPVSLLVSYLDGDQEREVSVDHGRIDPQQRILLSGVARLSLKQSLSRVELRLRSA
                     FMPQSVKIEEIFVQPVISNVA"
     gene            15244..15717
                     /locus_tag="VIO83_10050"
     CDS             15244..15717
                     /locus_tag="VIO83_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8331656.1"
                     /translation="MRIRWSSAVPLLCAALLLGGCSEADWGADQHGRPVAAAQLDGRW
                     LVINYWAEWCGPCRKEVPELNRLASELAAQPVRVLGVNFDGLQGEELRKATAALGIAY
                     TNLASDPAARLGLTSSPVLPVTWLVDPQGRVREQLAGEQTSAGLQAKLAALRAQP"
     gene            complement(15769..16722)
                     /locus_tag="VIO83_10055"
     CDS             complement(15769..16722)
                     /locus_tag="VIO83_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331657.1"
                     /translation="MPRRCHRFAGATLCGAVFLTGCANQLPQRSELEERVERKLLEHS
                     LQVDAGTPPVLELPQRHVRVLEQKTFEATPYEVTRRYQRYTPYQPWRELYEIPLGVVA
                     VLAGVGANVLNVPLLGSLPETATREWISYGFAGLNPFMNAESNGRAQQSLAALDEQQG
                     ETRLETSSLPWAERPVLVTAGKQTYELTTNRNGVLRLNLLDSPLAEQDVSRLGKLQLR
                     VEDPQDQTRTELTLPVSRDLRSRLQEAQPLIYEDLEEGDVAHWVHRVKRLAELGLEEE
                     AGELEQSLIELTRNDPELQQELIQALLKDAGRVAADRPQED"
     gene            complement(16731..18452)
                     /locus_tag="VIO83_10060"
     CDS             complement(16731..18452)
                     /locus_tag="VIO83_10060"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY8331658.1"
                     /translation="MRTSQYLISTLKETPTDAVVISHQLLLRAGMIRKLASGLYTWLP
                     MGLRVLRKVENIVREEMNAAGALEVLMPAIQPAELWQESGRWEQYGPELLRIKDRHGR
                     EFCVGPTHEEVITDLARNELNSYKQLPINFFQIQTKFRDEIRPRFGLMRGREFLMKDA
                     YSFHLNQESLQETYDRMHQAYCNVFTRLGLNFRPVQADTGSIGGTGSHEFHVLAESGE
                     DDIAFSDSSDYAANIEKAEAIPRETARAAASEELRLVDTPDAKTIDELVAQFGVAIAK
                     TVKTLVVRGAEEGTLVALLVRGDHELNEIKAANLAQVASPLVMASEAEIRAAIGAGAG
                     AGSLGPLNLNIPCVVDRSVALVSDFAAGANQDGKHYFGLNWERDLPLPEVADLRNVVE
                     GDPSPDGQGTLVIKRGIEVGHIFQLGTKYSEALKLTVLNEIGKPVTLTMGCYGIGVSR
                     VVAAAIEQNWDERGILWPEALAPFQIAIVPMKYENAAVKEAADRLYAELSAAGYEVLF
                     DDRDKKTSPGVKFADMELLGIPHRIVVSERGLAESVLEYKGRRDSEAQSVPLAELQAF
                     LAARINR"
     gene            complement(18617..19915)
                     /locus_tag="VIO83_10065"
     CDS             complement(18617..19915)
                     /locus_tag="VIO83_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015802 - basic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family porin"
                     /protein_id="HEY8331659.1"
                     /translation="MKWNLIALAVAAGSTQLALASQQSEAQGLVEDSSLSVLSRAMYM
                     NIDSRHGASNGSSESVNPAGYAEETGLGFHAIYESGFTQGTVGFGVDAFGLAGVKLDS
                     GKGRAGALDMQPGSDGRSEDTFSRAGAAVKARISSTTLKYGEQFVDLPVLSTDDSRLL
                     PESVEGFLATSREIEGLELNAGHFTALTAQNQMHHDSYEDDVGKLQSLDLLGASYAVS
                     DELNVAYYFSDVEVGGSDVRGFKKHYANANYTLPLAEEQSLNFDFNIYKTRFDNRDAS
                     DADNTIWSLAAAYTLGTHTFTLGHQRSSGDTGYYYGVDGGGAVYLGNSVQFSDFVGED
                     ERSWQARYDLDMAGYGVPGLSFMTRYVTGDQISTAAGEEGQEHEWNFETAYVIQDGVA
                     KDLSFTVRHAVWRANSAYNADYDVDQNETRLIVEYPLSIL"
     gene            20152..20850
                     /locus_tag="VIO83_10070"
     CDS             20152..20850
                     /locus_tag="VIO83_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HEY8331660.1"
                     /translation="MPLLRDGQNDFLATVGKDAELALHMALPESDADRRQQLEHFIGD
                     RFAAHYGARVRHFMPCLLGLYDAEQQLQAALGVRCGQQGPLFLERYLDVPVEQRIAEL
                     SGGSQPLRGQIAEVGNLASLAAGHARLMIVALTDQLATAGFRWVAFTGTLLLLNSFQR
                     LGLNLLHLGPADGARMGDELGDWGSYYATNPQVMVGDVRACHQRLQSMGIYRRLGYQS
                     HRPLDGEFAHVACG"
     gene            20834..22294
                     /locus_tag="VIO83_10075"
     CDS             20834..22294
                     /locus_tag="VIO83_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8331661.1"
                     /translation="MSPAVERFRQQLSCFAARVAVTCGERRWSYAQLLDEVAVRQAIL
                     QRGAVQRLAIALDNGPEWLFWDLAALFGGVPCVPLPGFFSPAQQRHVLDTSGVDTLVA
                     SNPQAFAALGFVAAGDGLCQRPVAAPAALPAGTAKITYTSGTTGQPKGVCLSADAMLS
                     VAQSIAQATASSVIERHLCVLPLAVLLENLAGNYTPLLLGASLEIPPLAEVGMRGASQ
                     FELPAFLASLARGRPNSLILLPQLLLALVVAAEQGLALPDSLRFIAVGGGRVAPQLLE
                     RAEALGLPVFEGYGLSECSSVVCLNTPEQRRIGTVGRPLAHAEVSLAEDGEVLVRGAR
                     LLGYLGEPAPADDWLPTGDLGHWDDGFLVLHGRKKHQFVTAFGRNVNPEWVEAELVQQ
                     LPIAQAWLHGEALPANVAVLVPRRGEIGDAQLQAAVDQVNAGLPDYARVHHWLRAEQP
                     FSEANGLATANGRLRRAALFQHYRSAIDTLMAGSNV"
     gene            22308..22988
                     /locus_tag="VIO83_10080"
     CDS             22308..22988
                     /locus_tag="VIO83_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEY8331662.1"
                     /translation="MSFYETLQEQTVAEREYLLAAPILRQVLAGGIGLDSYVAFLGQA
                     YHHVKHTVPLLMACGARLPERLEWLREAIAEYIEEEYGHQEWVLNDIRACGADAEAVR
                     RETPNLPTELMVSYVYDRIARDNPVSFFGMVNVLEGTSIALATQAAGIIQGHLQLPPQ
                     AFSYLTSHGSLDIGHVEFFKKLMNRLDDPADQAAVIHTSKVVYRLYGDMFRSLPLAAA
                     GENVNATA"
     gene            22975..23766
                     /locus_tag="VIO83_10085"
     CDS             22975..23766
                     /locus_tag="VIO83_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8331663.1"
                     /translation="MQLPECRILLTGATGGIGQQLAERLCAGGAHLLLVGRQAARLEQ
                     LVAQSHGRAESVCADITSSAGRDAVLQAARRFGGINVLINAAGVNHFSMLEQHDEEAL
                     AALIQLNVTATLQLTHRLLPLLRQQQRALLVNIGSTFGSIGYPGFTAYCASKFALRGF
                     SEALRRELADTRVKVMYIAPRATRTEMNEQSVVAMNTELKVAMDAPALVADAVVAAIG
                     RESEELYLGWPEKLFVRLNSLLPRVVDQALRKQLPIIQRFARSKS"
     gene            23791..24447
                     /locus_tag="VIO83_10090"
     CDS             23791..24447
                     /locus_tag="VIO83_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8331664.1"
                     /translation="MPKSIVRNLILGGALLFSLPSFALSDGGEQSLKRLQGRWAEINY
                     QLPETQREAAFARLADEAETALKAEPQAAELLVWRGIILSTWAGAKGGLGALDLVKQA
                     RGQFEAAIKLDARVLDGSAYTSLGSLYYQVPGWPISFGDDKRAEQLLKQALSLNPDGI
                     DANYFYGDFLLRQKRHAEAKVALEKALAAPPRPGREVADAGRRKEAQALLQKVQAAQD
                     "
     gene            24594..25253
                     /locus_tag="VIO83_10095"
     CDS             24594..25253
                     /locus_tag="VIO83_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8331665.1"
                     /translation="MRILLVEDDKALGEGIRTALKPEGYTVDWVQDGVSALHALSHES
                     FELCVLDLGLPRMDGLEVLRRLRTTGNALPVLVLTARDATTDRIAGLDAGADDYLTKP
                     FDVAELKARLRALLRRSFGRAQSALEYRGIRLEPDSQQVTYQGAPVNLPRKEFLLLHE
                     LLAQPGRVLTRDKLQQVLYGWNEEVESNALEVHVHHLRKKFFPELIRTVRGVGYLVDK
                     A"
     gene            25250..26719
                     /locus_tag="VIO83_10100"
     CDS             25250..26719
                     /locus_tag="VIO83_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8331666.1"
                     /translation="MMLRSIRSRTLGLVLGVLCVALSLISWRSYVDAQHEIEELFDAL
                     LAHSARLLEGMAGGDLSPSARAALQQALDAALRAGRGAEARLDGHPYESKLVFQVLDQ
                     QGRVQVQSAGAPTQLLARLLAQLQAAERLALPSGQPLAAVAQQLRGYHDVRLEGRVWR
                     VFLRQDQADRHWLLVAESQDVRDELVGKIVLRSLLPDLVGLPLLALLVWSAIGLGLRP
                     LEQVVRLIKARDAENLAPLTLAPLPRELEPMVAALNRLLLQVTSVLEREKRFLADAAH
                     ELRTPLAVLRIHAQNALEAPEAADRAAALQQLEGGVDRATRVVTQMLTLARLAPDALQ
                     LAMAELDLAPYVRAELAELIPLALEREQELVLDYDETADYRLVADAPSLGTLLQNLVG
                     NAVQYTPPGGQIAVRLQAEAQRVGLRVADSGPGVPAEQRAQLFRRFYRQGEGRGAGLG
                     LSIVQRIVELHGGEILLDQAALGGLEVIVWLPRNLPGGR"
     gene            26837..27271
                     /locus_tag="VIO83_10105"
     CDS             26837..27271
                     /locus_tag="VIO83_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY8331667.1"
                     /translation="MFALDPRLQQDTLHLGDFPLCRLLLMNDASYPWFILVPRREEVS
                     ELFQLDEADQRSLWRETTLLAALLKDVFKADKMNVATLGNVVSQLHMHVIVRRRSDAA
                     WPAPVWGRPPAVPYTEDGVKEVRDRLKLVLDSADFRFAAEAA"
     gene            27268..27474
                     /locus_tag="VIO83_10110"
     CDS             27268..27474
                     /locus_tag="VIO83_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEY8331668.1"
                     /translation="MSLELRVTELETRLAFQDDALQQLSDELLAQNRLIERLQRQIEA
                     LASRQADLAGQIGPVEDEAPPPHY"
     gene            complement(27544..28191)
                     /locus_tag="VIO83_10115"
     CDS             complement(27544..28191)
                     /locus_tag="VIO83_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY8331669.1"
                     /translation="MLKTVHLLSGVAALLLSFVPSLRGEALPWLQQPDALYLAFIGLT
                     NLLISPLVGASRQRVQPLSSALLVLAAVLQGIALLLPLDNLAGQPVLLYTLLAVAIAA
                     FLQLLPGQPAGQAQATAPTEQPAVRVSPAADAKPAPSVRRSAPSAGRETGTVKWFNTS
                     KGFGFISRDSGDDIFVHFRAIRGDGHRVLVEGQRVEFTVAQRDKGLQAEDVLILD"
     gene            28601..30376
                     /gene="aspS"
                     /locus_tag="VIO83_10120"
     CDS             28601..30376
                     /gene="aspS"
                     /locus_tag="VIO83_10120"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY8331670.1"
                     /translation="MMRSHYCGQLNESLDGQEVTLCGWVHRRRDHGGVIFLDIRDREG
                     LAQVVFDPDRADTFAAADRVRSEYVVKITGKVRLRPAGAVNPNMASGAIEVLGYELEV
                     LNEAETPPFPLNEYTDVGEETRLRYRFIDLRRPEMAEKLKLRSSITSSIRRYLDDNGF
                     LDVETPILTRATPEGARDYLVPSRTHAGSFFALPQSPQLFKQLLMVAGFDRYYQIAKC
                     FRDEDLRADRQPEFTQIDIETSFLDEDDIIGLTEKMIRQLFKEVLDLEFGEFPHMTYA
                     EAMRRYGSDKPDLRIPLELVDVEDQLKDVDFKVFAGPANDPKCRVTALRVPGAASMPR
                     KQIDDYTKFVSIYGAKGLAYIKVNERAKGVEGLQSPIVKNIPLDNLNVILDRVGAVDG
                     DIVFFGADKAKIVSEALGALRIKVGHDLNLLTCQWAPMWVVDFPMFEENDDGSFTALH
                     HPFTAPKCSPQELEANPGTALSRAYDMVLNGTELGGGSIRIHRKEMQEAVFRILGIDE
                     AEQQEKFGFLLDALKFGAPPHGGLAFGLDRLVMLMTGASSIREVIAFPKTQSAACVMT
                     QAPGIVDNKALRELHIRLREQPKAE"
     gene            30604..31350
                     /locus_tag="VIO83_10125"
     CDS             30604..31350
                     /locus_tag="VIO83_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8331671.1"
                     /translation="MAGHSKWANIKHRKGRQDAKRGKIFTKLIRELTVSAKGGASPAD
                     NPRLRLAVDKALTANMSRDVIDRAIARGAGTNEADNMAELSYEGYAPSGVAIIIEAMT
                     DNRNRTAAEVRHAFSKCGGNLGTDGSVAYMFDRKGQISFAPGVNEEALMEAALEAGAD
                     DIVSNDDGSVDVFTSFADFHAVNEALNAAGFKGDEAEVAMIPSISAPVADLETAQKVL
                     KLIDMLEDLDDVQSVYHNAEILDEIMEQLD"
     gene            31540..32064
                     /gene="ruvC"
                     /locus_tag="VIO83_10130"
     CDS             31540..32064
                     /gene="ruvC"
                     /locus_tag="VIO83_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY8331672.1"
                     /translation="MTLILGIDPGSRITGYGVVRDTGRGCEYVASGCIRTGDGALPER
                     LQKVYRGVREVIRLHGPVMLGIEQVFMSRNADSALKLGQARGAALVAAMEEGLSVAEY
                     TATQVKQAIVGTGAADKQQVQLMVMHLLKLVQKPQIDASDALAIALCHAHHRQSLLPH
                     GLSSARRRSGRLRL"
     gene            32080..32682
                     /gene="ruvA"
                     /locus_tag="VIO83_10135"
     CDS             32080..32682
                     /gene="ruvA"
                     /locus_tag="VIO83_10135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY8331673.1"
                     /translation="MIGRLRGTLAEKQPPHVLLEVGGVGYELEVPMSTLYRLPGVGEP
                     LTLHTHLVVREDAHLLYGFAEKRERELFRELIRLNGVGPKLALALMSGLEVDELVRCV
                     QTGDTKALTRVPGVGKKTAERLLVELKDRFQVWESMPGAPLVVEPARAASVSSAEADA
                     VSALVALGFKPAEASRAVALVDEDGLSSEELIRRALKGMA"
     gene            32701..33750
                     /gene="ruvB"
                     /locus_tag="VIO83_10140"
     CDS             32701..33750
                     /gene="ruvB"
                     /locus_tag="VIO83_10140"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY8331674.1"
                     /translation="MIEADRLVTASPREREEQQDRAIRPLKLAEYIGQPVVREQMELF
                     IQAARGRSEALDHTLIFGPPGLGKTTLANIIAQEMGVSIKSTSGPVLERPGDLAALLT
                     NLEPGDVLFVDEIHRLSPIVEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLIGA
                     TTRAGMLTNPLRDRFGIVQRLEFYSVADLATIVSRSAGILGLPIDAEGAFEIARRARG
                     TPRIANRLLRRVRDFAEVRGRGEISLSIADRALDMLDIDAHGFDHQDRRLLLTMIDKF
                     DGGPVGIDSLAAAISEERHTIEDVLEPYLIQQGYIMRTPRGRVVTRHAYLHFGLNVPG
                     RLGETVAAGQAGADD"
     gene            33791..34234
                     /gene="ybgC"
                     /locus_tag="VIO83_10145"
     CDS             33791..34234
                     /gene="ybgC"
                     /locus_tag="VIO83_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEY8331675.1"
                     /translation="MRAQNTGLSFTHRCRVYYEDTDAGGVVYYVNYLKFMERARTERL
                     RALGFVQSQLAGENLLFVVHSAEARYLAPARLDDELLVSAEVVELNRASLRFHQQVRR
                     AGDDALLCEGRVTVACVRADSFKPRAIPEALRAAFSGASPIPAGE"
     gene            34237..34932
                     /gene="tolQ"
                     /locus_tag="VIO83_10150"
     CDS             34237..34932
                     /gene="tolQ"
                     /locus_tag="VIO83_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEY8331676.1"
                     /translation="MEANAVDHMSMWSLISNASFVVQLVMLILVAASVTSWIMIFQRS
                     SLLRAAKKGLDSFEERFWSGIDLSKLYRQAGSNPDPDSGVEQIFRAGFKEFSRLRQQP
                     GVDPDAVMDGVNRAMRVAISREEEKLEQSLPFLATVGSTSPYIGLFGTVWGIMNSFRG
                     LAQVQQATLATVAPGIAEALIATAIGLFAAIPAVIAYNRFSSRGEMLINRYYTFADEF
                     QAILHRKVHTSDE"
     gene            35033..35485
                     /gene="tolR"
                     /locus_tag="VIO83_10155"
     CDS             35033..35485
                     /gene="tolR"
                     /locus_tag="VIO83_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEY8331677.1"
                     /translation="MARIRNRRKPVAEMNVVPYIDVMLVLLVIFMVTAPMLNQGVKVD
                     LPKVSSEALPQDNNAQVLTISIKADKTYYWNMGSEVDVDTAQDKALTLDEMVRAVTAI
                     MNQSRSQGKQVQVFVRGDKAVDYGAVMAAMGGLQQAGVGNVGLITEAP"
     gene            35485..36534
                     /gene="tolA"
                     /locus_tag="VIO83_10160"
     CDS             35485..36534
                     /gene="tolA"
                     /locus_tag="VIO83_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEY8331678.1"
                     /translation="MQQHERSPSASESYFWPTIWAVALHALIFAMLFVSFAMTPELPP
                     SRPIVQATLYQLKSQSQATVQTNQRIAGEAKKTAAPQYETEQMEQKKVEQQRVAAAKA
                     AEQKKAEQAAAAQKEAAAAKAEAQKQAEAAAKAEAAKKAAEQKQQADFAKKKAAEDAK
                     KKAEEAAKQKDAEEAAKKKSAEEAKKKAAEDAKKKAAEEAKKKAAAEAAKKKAAEQSA
                     RKAQEDKKAAALAELLSDDVQHQQALADEVGDQVAGSLDDLIVNLVSQQWRRPPSARN
                     GMSVEVLIQMLPDGTITNASVSRASGDAAFDNSAVAAVRNVGRIPEMQQLDRATFDKM
                     YRQRRMIFKPEDLSL"
     gene            36531..37835
                     /gene="tolB"
                     /locus_tag="VIO83_10165"
     CDS             36531..37835
                     /gene="tolB"
                     /locus_tag="VIO83_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEY8331679.1"
                     /translation="MNTLIRMALLGLVLLAGMAKAADPLVISSGTDRAIPIAVVPFGS
                     QGGAVLPEDMADIIGKDLRNSGYFEPIPRQNMISLPSQASEVIYRDWKALGAQYVLTG
                     SMVPSGGGVQIQYALFNVTTEQQMLTGSVGGTTAQLRDMAHFIADQSFEKLTGIKGAF
                     STKLLFVTAERFAANNTRYTLQRSDYDGARAVTLLQSREPILSPSFAPDGRRIAYVSF
                     EQQRPRIFVQNIDTGRREQITNFEGLNGAPAWSPDGSRLAFVLSRDGNPEIYVMDMGS
                     RQVRRVTNDQSIDTEPFWGKDGQTIYFTSDRGGKPQIYKTRVGGGGAERVTFVGNYNA
                     NPKLSADERTLVMVHRQDGFTVFKVAALDLQRGGAPRILSGETSLDESPTVAPNGTML
                     IYATRQQGRGVLMLVSVNGRVRLPIPAAQGEIREPSWSPYLN"
     gene            37911..38411
                     /gene="pal"
                     /locus_tag="VIO83_10170"
     CDS             37911..38411
                     /gene="pal"
                     /locus_tag="VIO83_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY8331680.1"
                     /translation="MEMLKFGKFAALALAMAVAVGCSSKGGDAAGEGAVDPNAGYGAN
                     AGAVDGSLGGEEAALRAITTFFFEYDSSDLKPEAMRALDVHAKDLQASGQRVVLEGHA
                     DERGTREYNMALGERRAKAVQRYLVLQGVSPAQLELVSYGEERALVAGSSEESWAQNR
                     RVELKK"
     gene            38418..39212
                     /gene="ybgF"
                     /locus_tag="VIO83_10175"
     CDS             38418..39212
                     /gene="ybgF"
                     /locus_tag="VIO83_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY8331681.1"
                     /translation="MHKHSRLLTLLALAAPLTTWAQVPVMDGSNGYPPSGYGATGAAY
                     AGGAATAPASAQGELFLQLQQLQEEVSRMRGTLEEQQYEIKQLKQESLQRYQDLDSRL
                     SGAGQAPAPAAQAPLAPAAADSASAGQAPAANAAPGDPAKEKLFYDAAFDLIKAKDFA
                     KADQAFSAFLRKYPTSQYAGNAQYWLGEVKLAQGDLQGAGQSFARVSQGYPDHQKVAD
                     SLYKLADVEQRLGNVAKAKGILQQVIAQYPGTSAAQLAKRDLARLP"
     gene            39333..39980
                     /gene="queE"
                     /locus_tag="VIO83_10180"
     CDS             39333..39980
                     /gene="queE"
                     /locus_tag="VIO83_10180"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEY8331682.1"
                     /translation="MNQTLRITEIFYSLQGEARTVGLPTVFVRLTGCPLRCQYCDTAY
                     AFSGGEIMTLEQILARVASYQPRYVCVTGGEPLAQPNCIPLLAALCDAGYEVSLETSG
                     ALDIAAVDTRVSRVVDLKTPGSAEVGRNRYENMAQLTRNDQVKFVICSREDYDWAVSK
                     IIEYRLEQRAGEVLMSPSHHQLEARSLAGWIIADNLPVRLQLQLHKYLWNDEPGH"
     gene            39982..40656
                     /gene="queC"
                     /locus_tag="VIO83_10185"
     CDS             39982..40656
                     /gene="queC"
                     /locus_tag="VIO83_10185"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEY8331683.1"
                     /translation="MSEKRAVILLSGGLDSATVLAMARAEGYACYTMSFDYGQRHRAE
                     LQAAERVAHQLGALEHKVVGINLNGIGGSALTDSTIAVPEEPAEGIPVTYVPARNTLF
                     LSLALGWAEVLGAHDIFIGVNAVDYSGYPDCRPEFIDAFERLANLATKAGVEGEGFRI
                     RAPLQYLSKAQIIQAGLARGVEYGLTVSCYQADADGRACGRCDSCRLRAEGFRAAGMV
                     DPTRYF"
     gene            40716..40790
                     /locus_tag="VIO83_10190"
     tRNA            40716..40790
                     /locus_tag="VIO83_10190"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40748..40750,aa:Lys,seq:ttt)
     gene            40956..42014
                     /gene="nadA"
                     /locus_tag="VIO83_10195"
     CDS             40956..42014
                     /gene="nadA"
                     /locus_tag="VIO83_10195"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY8331684.1"
                     /translation="MSQVSERLLVQAHLDAKQPRPLTAEEVADYRQAIAAELKARDAV
                     LVAHYYTDPILQALAEETGGCVADSLEMARFGSQHPASTIVVAGVRFMGETAKILNPE
                     KRILMPTLEATCSLDLGCPVEEFSAYCDQHPERTVVVYANTSAAVKARADWVVTSSCA
                     LEIVESLMDNGEKILWAPDQHLGRYIQRETGADMLLWEGACIVHEEFKARQLHDLKAL
                     YPDAAVLVHPESPEAVVALADAVGSTSQLIKAAQTLPNQIFIVATDRGILYKMQQLCP
                     GKSFIEAPTAGQGAACRSCAHCPWMAMNTLERTLQCLRTGSNEIHVDPALIPKAVKPL
                     KRMLDFTQAARLKLSGNA"
     gene            complement(42075..43508)
                     /locus_tag="VIO83_10200"
     CDS             complement(42075..43508)
                     /locus_tag="VIO83_10200"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY8331685.1"
                     /translation="MKILRPTLLTLACLLATPALASDLPSLGDASSSIVSPDQEHQLG
                     RAWLNVLRGQVKSLSDPLLEDFIENSVYRLAETSQLQDRRLVFVLLDSPQLNAFAAPG
                     GVIGVNGGLFLHAQTEGEYASVLAHELAHLSQRHFARGLEEQKRMQVPLMAAMLAGVV
                     AAAAGAGDAGIAAIASTQAAAVQAQRSFSRQNEQEADRIGILNLQQAGYDPNAMPSMF
                     ERLMRQYRYTRMPPEFLLTHPVSESRVADTRNRAEQLAGGGNTDTLNYQLMRARVQLL
                     YESSPGLAAKRFRALLDENPNLDAARYGLTLAQIKNGQLKEAASSLQPLLAKAPNDIV
                     YNLAQAQLDVAANRPDAASQRLQRLLGLYPDNYPLRQYQAEVLLKQNRPAEAEKRLDA
                     LLKERPGDAELWYQQAETRGLSGNIIGVHQARAEYFALTGDYDQALEQLDFARRRASS
                     NFQLAARIDARHSTLEEEQRMIDKLLR"
     gene            43614..43865
                     /locus_tag="VIO83_10205"
     CDS             43614..43865
                     /locus_tag="VIO83_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007963279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY8331686.1"
                     /translation="MTDTAGPAPAHDAELDASGLNCPLPLLKAKLELNRLPSGAVLKV
                     IATDAGSQRDFRAFARLAGHALLREEEEAGCFRYWLRKA"
     gene            complement(43894..44367)
                     /locus_tag="VIO83_10210"
     CDS             complement(43894..44367)
                     /locus_tag="VIO83_10210"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY8331687.1"
                     /translation="MPVAIDSPVADFQVQATSGQQVALGALKGRQLVLYFYPKDSTPG
                     CTTEGQNFRDLHAEFAAANTLIFGVSRDSLKSHENFKCKQEFPFELIADTDEQLCQLF
                     DVIKLKKLYGKEYLGIERSTFLIDKDGVLRQEWRGVKVPGHVDAVLQAARALNAG"
     gene            complement(44391..44948)
                     /locus_tag="VIO83_10215"
     CDS             complement(44391..44948)
                     /locus_tag="VIO83_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein R"
                     /protein_id="HEY8331688.1"
                     /translation="MSTPQPREQFLVISALGASPMELTSVLSRASQESRCNVVSSRLT
                     RHGEYSALVLQVAGSWDALARLEASLAPLAKRHKFSINVTRSAAAVERANALPYVVYI
                     SAAYRPDIIGELCQFFTDHHVELENLTCDTYQAPQTGTLLLNATITVTLPPGTQISWL
                     RDQFLDFADALNLDALIEPWRPQNP"
     gene            45120..45998
                     /gene="dapA"
                     /locus_tag="VIO83_10220"
     CDS             45120..45998
                     /gene="dapA"
                     /locus_tag="VIO83_10220"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY8331689.1"
                     /translation="MITGSMVALVTPMDAEGRLDWDSLTQLVDFHLQEGTNAIVAVGT
                     TGESATLDVDEHVAVIRHVVKQVAGRIPVIAGTGANSTREAIELTAAAKQAGADACLL
                     VTPYYNKPTQEGLYLHHKAIAEAVAIPQILYNVPGRTACDMLPDTIARLSTVPNIIGV
                     KEATGDLARGQEVLDKVAPGFLVYSGDDATAVELMLMGAKGNISVTANVAPRDMSELC
                     ALAMRGEAAPARAINERLMPLHKALFCEANPIPVKFALHEMGLIPQGIRLPLTWLSQG
                     CQEPLRQAMRQAGVLA"
     gene            46015..47106
                     /gene="bamC"
                     /locus_tag="VIO83_10225"
     CDS             46015..47106
                     /gene="bamC"
                     /locus_tag="VIO83_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEY8331690.1"
                     /translation="MKQLAGLSALALIIGTSSGCGWIWGDNGYFRDRGSDYLAARETA
                     PMQLPPDVQARRIEPLLPVPASVATRSAEGEFEVPRPQGLQATAQRSDFSLQRSGERR
                     WVVAQRSPAEVWPLARQFFEENGLSISEERPETGELVSAWQTVPTALARHLDKADAEV
                     SVRLRVEPGVQRNTSEIFLLSNVRPAGSSAEPAWPARSVQPALDGALIERLQSSLSSS
                     VGQGGSVSLLADRSFDAPKRVSLGSDGSGNPQLSLATDFDRAWSAVGRALQDGDVRVD
                     DMNRSLGIYYINLAESADKPGEEPGFFAGLLGGKADAEDVESRAERYHVRLTTVGDSV
                     QVSVEKDLETVAPADVARRVLELIQEHLG"
     gene            47194..48063
                     /locus_tag="VIO83_10230"
     CDS             47194..48063
                     /locus_tag="VIO83_10230"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY8331691.1"
                     /translation="MTTPTTLSLKKIYSGKVRDLYEIDDKRMLMVATDRLSAFDVILA
                     EPIPEKGKILTAISNFWFDKLKDLVPNHFTGDKVEDVVPAAELPLVEGRAVVAKRLKP
                     VAVEAIVRGYIVGSGWKEYQKSGTVCGIALPAGLKEAAKLPQPIFTPSTKAAVGDHDE
                     NISFEQCEAIIGKELAAKVRDTAIALYSAAVEYAATRGIIIADTKFEFGLDEDGTLTL
                     MDEVLTPDSSRFWPADSYVEGQNPPSFDKQFVRDWLESTGWNKEPPAPAVPAEVAQKT
                     ADKYREALTRLTR"
     gene            48161..48250
                     /locus_tag="VIO83_10235"
     tRNA            48161..48250
                     /locus_tag="VIO83_10235"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48195..48197,aa:Ser,seq:cga)
     gene            48445..48771
                     /locus_tag="VIO83_10240"
     CDS             48445..48771
                     /locus_tag="VIO83_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331692.1"
                     /translation="MNEKLFFLASKKRSTNHILHLILTLLTFGVWVIVWVIVASNNSS
                     YNSRIDAEMGRIISYKEKGLSDVQAYQQIRADKTKESQDRAKTVMVVFGVIVLFVILG
                     SLGVTP"
     gene            complement(48869..49567)
                     /locus_tag="VIO83_10245"
     CDS             complement(48869..49567)
                     /locus_tag="VIO83_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003138368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY8331693.1"
                     /translation="MEVVAYYRVSTKRQGESGLGLEAQREYVSTAAAQHGWVVVAEYT
                     DVNVSGSVHPLERPEAAKAFAHGLPVVVAKLDRLSRDVEHIAGLMKRSDFKVATMPAA
                     KTLELHLYAMLAQQEREFISQRTKEALQSLSKRAEAGDRVAQGKVANRAVSLAKGRTK
                     ANQMKGGIATQERAEVFAESLRDTVELCVLRGTRTLKGVADCLNAKGVKTARGGEWSP
                     VQVSRVMQKLSLSF"
     gene            49715..50248
                     /locus_tag="VIO83_10250"
     CDS             49715..50248
                     /locus_tag="VIO83_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331694.1"
                     /translation="MSNATPAVILGGAIMAAAAIYVAPSVIDDYQNQNIIITTNGAVR
                     LGKVYNEALKVRMEMKNTETGEVFISVDEDVDGFYKQARSELEKLIHATNEGAKSDKE
                     KISVEAAGLKVHMTITLDTYFLYRSEYQPSFSLTVDQHEISAGVNSGINKVLMQTQEY
                     LNEVKIENLEKNRFLKL"
     gene            complement(50388..50675)
                     /locus_tag="VIO83_10255"
     CDS             complement(50388..50675)
                     /locus_tag="VIO83_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331695.1"
                     /translation="MINQQFVSLSAQRTHELILERFHPERHQELLQEKESAKLAAEKE
                     ARIQALLDEYKANKAQATHKPSQQIDTTNKPAKKLDHKDSIKNMFKNKKAR"
     gene            complement(50734..51219)
                     /locus_tag="VIO83_10260"
     CDS             complement(50734..51219)
                     /locus_tag="VIO83_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331696.1"
                     /translation="MSKTIIDGQEITVKMPATFDPKELKARQDQAVTDYLNLPNITIF
                     TIKAEILFQALDLLEQKLQEGYQRARETNHQSHPFVIPMFKKPEEVEAELNTVRQQVE
                     ADYRSEIAAQVDEAKQNLIIQRKAAIQRQEDEKKRKADEKLHRELEKEAADLFDSYLP
                     K"
BASE COUNT         8535 a        16403 c        17624 g         8969 t
ORIGIN      
        1 cggcgcgagg cgccctgtgc gctggccggc cgctgcggtg gctgcatcct gcagcacctg
       61 tcgcatgccg agcaggttgc cctgaaacag cgcgcgctgg ccgagcagct gcaccgtctt
      121 gcggcgctgg cgccgcagga atgggcgccg ccgctgctcg ggccggagtt cggctaccgg
      181 cggcgggcgc gcattgccgt gcgctacagc cccaaaagtg ggcagctgga ggtcggtttt
      241 cgggcggcgg caagtcaggc gatagtcgag ctggaccagt gcgcggtact ggtacaggct
      301 ttgcaaccgc tgttcgccgc gctgcccggg gtactgcgcc gcctgcgcaa gccgcaggcc
      361 atcggtcatg tcgagctgtt ccacggcacc gccagtgcgc tgctggtacg ccatacccag
      421 gggctggcgg aggaggatcg ccagctgctg cgcgccttct gcagcgagca gggcgcccag
      481 ctgtggtggc agggcgaagg cgagccgcag gcggacgatc ccgcccagcc gctgggctac
      541 cggctggagg cctgcgattt gaccctggcc tggcggccgg gcgacttcgt ccaggtcaat
      601 gcgccggtca acgagctgat gctggcgcag gcgttggagt ggctggcgcc gcaagccgac
      661 gagcgggtgc tcgatctgtt ctgcggcttg ggcaacttcg ccctgccgct ggcgcagcgc
      721 gccgcggagg tggtcggcgt ggagggcgtg caggggatgg tcgagcgtgc cgctgcgaat
      781 gcgacggcca acggcttggg caatgcgcac tttttccgcg ccgacctgtc cgagccactg
      841 gcgcaggcgc cgtgggccaa gggcggtttt gccgccgtac tgctggatcc gccgcgcgat
      901 ggcgcggcgg cggtagtcgg ccagctgcgc agcctcggcg cgcagcgcgt gctttatgtg
      961 tcgtgcaacc cggccacgct ggcgcgcgac gcggcaatac tggcgcagca gggctatcgc
     1021 ctgaagcgcg ccgggatcct cgacatgttc ccgcagacgg cacatgtcga ggcgatggct
     1081 ttgttcgaag cgggcgcgga gcgtccgtag cgggtcgcag atagagccgg catcagccgt
     1141 cgctgtttca cgacctgtcc ataaccgacc cgcagaaggc cggctcaatc aggatctgca
     1201 gcagattctg gaccagacgt ggcggctgcc gcgtcgtggc aggaaaggta gaagacgatg
     1261 gtacaggtaa gagcggaaca gccgatcaac accgacggca gcatcaacct cgaggcctgg
     1321 ctggatcaca tcctcgatgt cgatccggcc cttgatcgcg acagcttgcg caaggcctgc
     1381 gaggtcgccc gcgaggccga gcagcaggcg atcgccgtcc agcacatctg ggccgaaggc
     1441 atgtccagtt tccgcaccgg gctggaaatc gccgaaattc tcgccgacct caagctcgac
     1501 caggaatcgc tgattgccgc ggtgctctat cgcggcgtgc gcgagggcaa ggtgcagctg
     1561 gaggtggtgg agaaggactt cggcgtcccg gtggccacgc tgatcgaggg cgtgctgcgc
     1621 atggcggcga tcagcaccag tctgagcccg cgccagtcgc tggtgctcgg tacccaggcg
     1681 caggtggaga acctgcgcaa gatgctggtg gcgatgatcg acgacgtgcg cgtggcgctg
     1741 atcaagctgg ccgagcgtac ctgtgccatc cgcgcggtga agaatgccga cgacgacaag
     1801 cgctaccgcg tcgcccgcga agtgttcgac atctacgcgc cgctggccca tcgcctgggc
     1861 atcggccaca tcaagtggga gctggaggac ctgtcgttcc gctacctgca gcccgagcag
     1921 tacaagcaga tcgccaagct gctgcacgag cggcgcctgg atcgcgagca gtacatcaac
     1981 caggtcatgc agcagctgcg cgacgagctc acggccaccg gcatcgaggc cgacatcagc
     2041 ggccgggcca aacacatcta ttcgatctgg cgcaagatgc agcgcaaggg cctcgagttc
     2101 agccagatct acgacgtgcg cgcggtgcgc gtgctggtgc cggaggtgcg tgactgctac
     2161 accgcgctgg gaatcgtgca caccctgtgg cggcacattc ccaaggagtt cgacgattac
     2221 atcgccaacc ccaaggagaa cggctaccgc tcgctgcaca ccgcggtgat cggccccgag
     2281 ggtaaggtgc tggaggtgca gatccgcacc caggccatgc acgaggaggc cgagctgggg
     2341 gtgtgcgcgc actggctgta caagggcacc gacgtcaatt ccggctccag ccactacgag
     2401 gagaagatcg cctggctgcg ccaggtgctc gaatggcacg aggaactggg cgacatcggc
     2461 ggcctggccg agcagctgcg cgtcgacatc gagcaggacc gggtctacgt gttcacgccc
     2521 gacggccatg ccatcgacct gcccaccggc gccacgccgc tggacttcgc ctatcgcgtg
     2581 cacaccgaga tcggccacaa ctgccgtggc gccaaggtca acggcaggat cgtgccgctc
     2641 agctacagcc tgcagaccgg cgagcaggtg gagatcatca ccggcaagca cggcgcgccg
     2701 agccgcgact ggctcaaccc caacctcggc tacgtgacca cctcacgggc gcgggcgaag
     2761 atcgtccact ggttcaagct gcaggcccgc gaccagaacg tgatcgccgg caagagctac
     2821 ctcgagcgcg agctggcgcg tctggcgctg acggcggtcg acgccgagcg gctggccgag
     2881 aagtgcaacc tgaagagcac cgaggacatg tacgcggccc tcggtgccgg cgacctgcgc
     2941 ttggcccagg tggtcaacgc cgcgcagcag ctggtcgagc cggagcgcgg caacgaaatg
     3001 ctcgacctgc tgtcgcggcg cggtcccagc gggcgcagcg gcgggcgcga cgagatccag
     3061 atcctcggcg tcggcaacct gctcacccag atggccggct gctgccagcc ggtgccgggc
     3121 gagccgatcg tcggctacat cacccagggc cgcggggtga gtatccatcg ccaggactgc
     3181 ccgatggcct tgcagcagac cgagcgcgag cccgagcgga tgatccaggt cagctggggc
     3241 caggcaccgg cgaagaccta cccggtggat atccagatcc gcgcctacga ccgctccggc
     3301 ctgctgcgcg acgtgtcgca gatgctgctc aacgagaaga tcaacgtgct ggcggtcaac
     3361 acccagtcga acaagagcga caacaccgcc aacatgagcc tgaccatcga gatccccagc
     3421 atggcggccc tcggtcgcct gctggcgcgc atctcgcagc tgcccaacat catcgaggcg
     3481 cggcgcagcc gctcgggggc ctgaggtgga catgtaccga ctggacgacc tgctgcacct
     3541 gatggcgcgc ctgcgcgacc cgcagagcgg ctgcccgtgg gatcttgccc aggactacgc
     3601 cagcatcgtg ccgcacaccc tcgaggaagc ctacgaggtg gccgacgcca tcgagcgcgg
     3661 cgacttcgcc cacctgccgg gcgagctcgg cgacctgctg ttccaggtgg tctactacag
     3721 ccagctgggc cgcgaggagg gccgcttcga cttcgccgcg gtggtcgacg gcatcacccg
     3781 caagctggtg cgccgccatc cccatgtgtt ccccgacggc gacctgtacg gcgcggcgga
     3841 tctgccgcag ctcgacgagg cggcgatcaa gcggcgctgg gaggagatca aggccgagga
     3901 gcgcgccgag aaggccgctg cccctgagca gctgtcgctg ctcgacgaca ttccgcaggc
     3961 gctgccggcg ctgtcgcggg cggccaagct gcagaagcgc gcggcccagg tcggcttcga
     4021 ctggccggca ccgctgccgg tgctggacaa gctgcaggag gagctcgacg aggtgctcga
     4081 ggcgatggcc gagggcgacc aggagcacat cgccgaggag ctcggcgacc tgctgttcgt
     4141 ggtggtcaac ctggcgcgcc acctcaaggt cgagccggag gcggcgttgc gcgccgccaa
     4201 cggcaagttc gagcggcgct tccagttcat cgagcgggcg gtgcgcgcca gcgggcgggc
     4261 gctgggcgac tgcagtctcg acgagctgga cgccctgtgg ggcgaggcca agcgcgccga
     4321 gcgccagcag acgggctgcg gcggctgagg ggcgcaggcg ggaagggagc ggggccgtgg
     4381 cggccccgca gcaggacgat cagacgatcc agcgatacag cagcacggcg atcgccacgg
     4441 cggcgatcgg gcgcaggacg cggtaggcct tgggattctt gcgcttgaac tggcggatca
     4501 ccccgccaac ggcgttgccc acccgcttga agccgctgta ggcgcggttg atcgcgccga
     4561 cccgctcgcc ctcgacgttc tgcggcgcgg tggcgcggcc cagggtgcgg ctgatccagc
     4621 ggttgaagcc ggccagcggc tccggcagcg gacgctcgac gtcgcagaac aggatcagtc
     4681 ggtcggcgtc ggtctcgttc ttcacccagt gcacgtaggt ctcgtcgaac atcatgtcct
     4741 cgccgtcgcg ccaggcgcgc ggctgtccgt cgacgaagat gcggcaggcg tcggagttcg
     4801 gtgtggccag ccccaggtgg tagcgcagcg agccgccgaa ggggtcgcgg tgcgggttga
     4861 gatggctgcc ggccggcagc atggtgaaca tggcgccctt caccgagggg atctgcccca
     4921 gcagctcgag ggtcttcggg cacagcttgg cggccgaggg cagcggctgc tcgtcgtacc
     4981 aggtcaggta gaaacgcttc cagccgcgct tgaagaacga accgaaaccg gcctcgttgt
     5041 cgtgcagggc ggcgcggata tagccctcgt cgaacagcgc cagggcctcc tcgcggatgg
     5101 tctgccagtt gtcgcgcagc aggtccagtt cggggaagtc gcggcgctcg atgtagggct
     5161 gcgagggcgt gcgcgagaac aggtacatca gcgagttgta cgggctgaac agcgacgagt
     5221 ggttgaccat ctgacgcagc agcggcaggc gggcctggcc gcgcaggtgc acgtacaggt
     5281 tgctggcgac gaaggcgccg agcagggaaa ccttgatgag ggttgcaggc atggcctagt
     5341 ccatcatggc ggaagcgaag ggggcaatta tgggcctctg tgtccggcaa acaccagtgg
     5401 cggggtggct aatgcggcat aatgccgtgc cgagacgatg gggggcttgc gcaacgcatg
     5461 ggcaagccgg aggatcggcg ggctgcaaac gcctgtggct ccccctctcc tgaattgaat
     5521 gaacgcccgg cggcgccggg ctgttggatg atcctttacg atgagcatgt cattgcgcga
     5581 ccagctgctc aaggcgggac tggtcaacga gaagcaggcc aaacaggcca ccaagcagaa
     5641 gcagaagcag cagcgtctgg agcacaaggg ccaggcggag aaggacgact cgcagcgcca
     5701 ggccgcgctg caggcgcagg cggagaaaac tgcccgcgac ctggagctga accgccagca
     5761 gcaggaaaag gtgcagaaga aggcgctggc cgcgcaggtc aagcagctga tcgaaagcag
     5821 ccgcctgccg cgtctggagg gcgacgacta ctacaacttc gtcgacgaca agaaggtcaa
     5881 gcgcatcacg gtcaacccga tgctgcgcga caagctcagc cgcggcacct tggccatcgt
     5941 gcgcctggag ggcaagtacg agctgatccc gcgcgaggcg gcgctgaagg tgcaggagcg
     6001 cgagccgcag cggatcatcg tgctcaacgt cgccagcgag gtgccggacg acgaggacga
     6061 tccgtacaag gattacgtgg tgcccgacga tctcatgtgg tgaaagcctg ttcacgaccc
     6121 gctgcgcgtc ggccatgctg cgttgcaatc ggtttcggaa tgctcatttg caatcagcaa
     6181 actccgcttc ctcaaccgat tgcgccttgc ctggctctag ctcgcaggct cgtgagcagg
     6241 ctttgaaaga gtggcttcgg cagaatgaaa aacgcccggc atgcgccggg cgttttcatg
     6301 tgcgggcagg cttacttgcc ctgcacgttg cggccgttga ccgcgccctg cttgagcatg
     6361 atctggtatt ccttgccgtc cttttccacc tggtgcaggc gcaccagcag atggccccag
     6421 tccttggcga accacagcac ggtctggcga ttggacttgg tggggtcgcg cacgcgttcc
     6481 accttgagcg cgtcgatctg tccggcctcg gtctgcacct gttcctcgcc gagcacgcgg
     6541 aagtcgtagg tttcgatctc gtcgccgtcg agcacctggt aggtcatgct cttcttgccg
     6601 gcggcgacat cttcctgcag ggccagctgg taggtcgact tgtccagcag gccgcttttc
     6661 agcaccagtt tcaccggcgt gtcgcggtcg ttgccggtca ccagcttggt cttccagtcg
     6721 aagtcgtggg cgaccttctt gctcttgccc aggccgttgc gttcgaagcg gtagctgagc
     6781 ggcacgaagc gctggttgtc gtagcggaaa cgactcactt cggtcagccc ggacaccagc
     6841 atcgaagcct tgaactgcag cgtccagctg ccgtcggcgc ccttgtccag ctggcgctcg
     6901 gccgtgccgc tgaccggcac ctgctgccag tcggcggtgt actgcgcggt gaagggctcc
     6961 agggccaggg cgctggctgg caggccgaac aggaccaggg caagcgttaa cgcacgtcgc
     7021 atggcaaatc tcctgtggtg acagaccgtt gccggcggcc tcaacgaggc aggggcaggt
     7081 cgcgaatttg ctggccgctg gccggcaggg gcacgccgtc cagttccgca ccatggttgg
     7141 cgagacgcag gcggccttca gcgaaccagc gcaccgccag cggatagatg tgatgctcgc
     7201 cgatctgcac ccgcttggcc agcgcctcgg ggctgtcgcc gggctgcacc ggcaccacgc
     7261 cctgtacgac cagcgggccg ccgtcgagtt cctcggtgac gaagtgcacg ctgcagccat
     7321 gctcgccatc cccggcctcc agcgcacgct ggtgggtgtg caggcccttg tgtctgggca
     7381 gcagcgacgg atggatgttg agcaggcggc cgtggtagtg gtggacaaag ccggcgctga
     7441 ggatgcgcat gaagccggcc agcacgacca ggtcgggctg gaaggcgtcg atggtctcga
     7501 tcagcgcggc atcgaaggcc tcgcggcctg cgaacgcctt gtgatccagc acggcggtgg
     7561 cgatcccggc gttgtgcgcg cgggtcagcc cgtaggcatc ggcgcggttg gagatcaccg
     7621 cgcagatgcg tgccgggttg tcttcgccca ggctgtcgat cagcgcctgc aggttgctgc
     7681 cggagccgga gatcagcacc accacattgc agggctgggc catcagtggg ccttcaggtt
     7741 gttcagcacg acgcgctcgg caccctcggc gccgctggcg atgctgccga tcacccacgg
     7801 ctgctcgccg ctggcgcgca ggttggcgag gacggcttcg acctggtcct gggcgacgca
     7861 gatgaccatg ccgacgccgc agttgagtac gcggtgcatc tcgtgctcgt cgacgttgcc
     7921 ctgctgctgc agccagtcga acaccgccgg tcggttccag ctggccacgt cgatgatcgc
     7981 ctgggcgcct tcgggcagca cgcgcgggat gttgtcgagc aggccgccgc cggtgatgtg
     8041 ggccatggcc ttgaccgcac cggtgtcctt gatcagcttg agcagcggct tgacgtagat
     8101 gcgcgtcggc gccatcagca gctcggtcag ggccttgccg tcgagctgga tctgctcgat
     8161 gtcggcgccg gcgacttcaa tgatcttgcg gatcagcgag tagccgttgg agtgcgggcc
     8221 ggaggagggc agggcgatca gcgcgtcgcc ggcggcgact ttcgagccgt cgatgatctc
     8281 gctcttttcc accacgccga cgcagaagcc ggccagatcg tagtcctcgc cttcgtacat
     8341 gccgggcatc tcggcagttt cgccgccgac cagcgagcag ccggccagtt cgcaaccggc
     8401 gccgatgccg gtgaccacgg tggcggccac gtcgacgttg agcttgccgg tggcgtagta
     8461 gtcgaggaag aacagcggct cggcgccgca caccaccagg tcgttgacgc acatggcgac
     8521 caggtcctgg ccgatgctgt cgtgcttgtt gaggttcagc gccaggcgca gcttggtgcc
     8581 gacgccgtcg gtgccggaga ccagcaccgg ctgcttgtag ccggccggga tttcgcacag
     8641 ggcgccgaag ccgcccaagc cgcccatcac ttcagggcgc gcggtgcgct tggcgacgcc
     8701 cttgatgcgt tcgaccagtg cttcaccagc gtcgatgtct acaccggcgt ccttgtagct
     8761 cagggagggt tgcttgctca taaatccggg cctttgaagg aagtgcgtga aagcccgcga
     8821 ttttagcatt cttgcaagat agcggccatc atgggcggtt gccgccatcc gggcggctgt
     8881 ttaaggtata ggctttgttt gttgcgcgcg tctggcgcgc gcctgtcccc ttgccgagat
     8941 gctcgcatgc gcctgatcgc ccgatggttt gccttctgcc tgttgttcgc caccctgtcg
     9001 gccacggctg cccaggttga gggtctgtac gaggtgcacg agccggtgac gtcgcaggat
     9061 ccggcggagc gggcggcggc catgagccgt gccctgcaga ccctggtggt gcgcctgacc
     9121 ggcgatcgcc aggcggcgca gaacccgcgg ctgcaaccgc tgctggccga tccgcagcag
     9181 ctggtgcagc agtatgtcta cgagtccggt acgccggtca ccctgtccgt ggtgttcgat
     9241 ccggtgctca gccagcgcgc cctccgcgag gccggcctga ccctgcagac tgccgagcgg
     9301 gcgagcgtgc tggcctggtg gctgaacgag gcggatggcg ccagcagtct ggtggccgag
     9361 gatcagccag tggcggcgac cctgcgcgcc gcggcgcaga accgcggcgt gccgctgagc
     9421 ctgccgctgg gggacctcga cgaacaactg ctggccacgg ccgataccct ggcgggcagc
     9481 gatgcccagg cgctgcagga ggcttccgcg cgctacgcag cggacgccct gctggccgtg
     9541 gtggcccgcg gcgccggcga cagctggcag gccgactggc agctgtgggt cggcagcgag
     9601 cgggtaacgg gttcgagcac tgccgccagt caggcggcgc tggctgatgc cgtactcctc
     9661 gaggtgggtg aaaagctggc ctcgcgcttc agcaaggccg gcgcggcgca gagtctgacc
     9721 ctcgaggtgc agggcagcga tctggcccgc tatgcggcgc tcgagcgtct gctcgagccc
     9781 tttgccccgc gcctgctcga ggtctccagt ggcgggcgcc tggtctatca ggtcgacgcc
     9841 agccccgatc agctgcgttc ccagctgcag ctgggccgtc tgcacgaagt gccggccgag
     9901 gccgtcgatg cccagggcgc tgtcccggca gcgaacccgc cgctgcaggt accggcggaa
     9961 acggccaagc gcctgcgttt cgcctggtaa gcccgcggcc aggcggccgc gtcatggatt
    10021 gatgagtaga gggcgcaaat gatgctgaat gattcccgca gctggctggg gctggcggcc
    10081 ctggtgctgc tcggctggct ggtccaccag ctgcagccgg tactgatgcc gttcctggtg
    10141 gccctgctgc tggcctatct cggcgatccg ctggtcgatc gcctcgaggc gcgcggcctg
    10201 tcgcgcacct cgggcgtggt ggtggtgttc atcctgttca gcctgatcct gctggccctg
    10261 ctgctggtgc tggtgccgct gctcggcaag cagctggtgc ggctgtacga gctggcgccg
    10321 gagatgctcg actggctgca ggggactgcg ctgccctggg tgcagaagaa gctgggcctg
    10381 cgctacgcct tctggcgttt cgaccggatc aagacgctgc tcgccgaaca tctgggccag
    10441 gccaccgata tcgccggtat cctgctggcc caggtcacct cgtcgagcct ggcgctgctg
    10501 gcctggctgg gcaacctgct gctgatcccg gtggtgagct tctacctgct gcgcgactgg
    10561 gacgtgatga tgggccatct gcgcaacctg ctgccgcgca agcgcgagga gtgggtggtg
    10621 cgcctggccg gcgagtgcca cgaggtactg ggtgccttcc tgcgcggcca gctgctggtg
    10681 atgctggcgc tggcgctggt ctatgccggc gggctgatgc tggtcggcct tgagctgggc
    10741 ctgctgatcg gcctgctcgc cggactggcc agcatcgtgc cgtacatggg cttcgtggtc
    10801 ggtatcgtcg ctgcgctggt cgcggtactg ttccagttcg gcctcgactg gtatccgctg
    10861 ctcggcgtgg tggtggtgtt cggcatcggt cagctgctcg agggcatgtg gctgacgccg
    10921 ctgctggtgg gtgaccgcat cggcctgcat ccggtggcgg tgatcttcgc catcctggcc
    10981 ggcggccagt tgttcggctt caccggcgtg ctgctggcgc tgccggtggc ggcggtgatc
    11041 atggtgctgc tgcgccacat gcacgagttc tataaactgt cgagacttta cggagagtcg
    11101 ggtggcgact cgcaggagtc gtcggagtcc ccatgaaacc catccagctg cctctgggcg
    11161 tgcgtctgcg tgatgacgcc accttcgcca actactatcc cggggccaac gcggccgcgc
    11221 tgggctatgt cgagcgcatg tgcgaaaccg acgtcggctg ggcggaaaac ctgatctacc
    11281 tgtggggcgc cgaaggcgtc ggccgcagtc atctgctgca ggcagcctgc ctgcgcatgg
    11341 agcagttcgg cgggcgcgcg gtgtacctgc cgctggatac ggtggcgatg tacgggacgc
    11401 agctgctcga caacctcgag cagtgcgatc tggtatgcat cgacgaggtg gatctggtcg
    11461 ccggccaccc gacctgggag gaggcgctgt tccacctgtt caaccgcctg cgcgatgccg
    11521 gccggcgcct gctgctgtcg gcctcggcgc cgccgcggga agtgccgatc aagctgcccg
    11581 acctcaagtc gcggctaacc ctggcgctgg tgttccagct gcacggcctg tccgacgagg
    11641 acaagctgcg cgccctgcaa ttgcgcgcct cgcgccgcgg cctgcacctg accgacgagg
    11701 tcggccgctt catcctcacc cgcggctcgc gcagcatgaa ctcgctgttc gacctgctgg
    11761 agcagctcga caacgcctcg ctgcaggcgc agcgcaagct gaccattccc ttcctgaaag
    11821 aaaccctggg ctggtgaggc tggcgtaggg tggctcaggc cgcaggccgt aacccaccga
    11881 tcgatcgtca ggctggccat tgcgatgctg gcggcatcgg tggtgggtta cgccgcgttg
    11941 cggctaaccc accccacgca tcactccccc gccagcttgc ggtcgtactg gaagcgccag
    12001 cgggtgtaga gcagggcgct gcagaacagg gtcagactca ggccggcttc cagccagcca
    12061 aacagcgcgc ggtcgggctg gaaggcggcg accacgccct ggatgaagta caggttgagc
    12121 acgtagcagg tccaggcatg cacccgcgcg ttgcccgtca gcatgcccgg cgccaggatc
    12181 gccagtggca ccaggtgcac gccgaggatc acccaggggc gggcaccgtg caggtcctcc
    12241 agcagcaggt accagacgcc gagcagcacc accagggcca cgaacagcgc caggctgagc
    12301 cagcgcgcca ggcgtacgcg cggttccagc cagtccagcg ccggcagtgg tttgttcttc
    12361 ttggccatct cagttcccca gtttctgcgc ggtgtgcgcc aggcgctgcc ccagtgcgcg
    12421 gcacagggcg gtttcgtgtt cgtcgagcgg gcgcttgccg tcgttgccgg catggtggct
    12481 ggcgccatag ggcgtgccgc cgccacgggt atccagcaga gcctgctcgc tgtagggcag
    12541 gccgcagacc agcatgccgt ggtgcagcag cggcagcagc atggacagct gggtgctttc
    12601 ctggccgccg tgcaggctgg ccgtggaggt gaacaccccg gccggcttgc cgaccagttc
    12661 gccggtcatc cacaggctgc tggtgccatc gaggaagtgc ttgagcgggg cggccatgtt
    12721 gccgaagcgg gtcgggctgc cgagggccag gccggcgcag tggcgcaggt cgtcgaggct
    12781 ggcgtagagc gcgccttgct cgggaatttc cggggccacg gcggcacatt cggcggacac
    12841 cgccggcacc gtgcgcaggc gcgcctccag gccgccctgt tcgacgccgc gggcgatctg
    12901 ccgagccatc tcggcggtgg cgccgtggcg actgtagtag agcaccagga tgtagggggc
    12961 actcacgcca gcagctccag cacgcgttcc ggcggccggc cgaccaccgc cctggcgcca
    13021 accaccagga tcggccgctc gatcaggcgc gggtgcaggg ccatggcggc gatcagcgcc
    13081 tcgtcgtcga gtgccgggtc ggccaggttg agctccttgt acagctcctc gccgctgcgc
    13141 agcagctggc gcgcgccgta gccgagcttg ccgagcagct cgcgcagttc ggcggcggag
    13201 ggtggcgttt ccaggtagtg catcacccgg ggctgcaggc cgcgcgcttc gagcagctcc
    13261 agggcgccgc gggatttcga gcagcgcggg ttgtggtaca ggatcatgtc ggtcatgtgg
    13321 gattcgcctg gccgtgattg agggcgctat tctaaccgcg tcggcgcgtg ccccagaacc
    13381 ttcctgcggc ggcgaggtgc ggcacggtca gtcacaagga gagtcgatga atcgtcatct
    13441 gtaccacctg tggtgtttcg tcgagcggct gatccgccgt ttcttcgccg atcgcgggct
    13501 gcagagcgcg gcggcgctga cctacaccac gctgttcgcg gtggtgccga tgctgaccgt
    13561 gaccttcgtc atgctcgctg cggtgccggc cttccgcgat ctgggcgagc cgatccaggc
    13621 cttcatcttc cgcaatttcg tgccctcggc gggcgagcag gtgcagcagt acctgggcgg
    13681 cttcatcagc caggcgcgcc agctgacctg gatcggggtc gccgtgctgg tggtcagcgc
    13741 gctgctgacc ctgagcacca tcgagggcgc cttcaaccat atctggcggg tccggcgggc
    13801 gcggcgcggg gtgtcgagct tcctgctgta ctgggcgatc ctcagccttg gcccgctgct
    13861 gctgggtgcc ggttttgccg ccagcaccta tgtcacctcg ctcgagctgc tgtccggccc
    13921 gcacgcgctg ccgggggcgg cgaccctgct cggactgatg ccgctgctga ctggcatcgc
    13981 cgccttcacc ctgctgtatg ccgccgtgcc caatacccag gtgtccggcc gccatgcact
    14041 gctcggcggc tgcttcagtg ccctgctgtt cgaggccgcc aaggcgctgt tcgccctgta
    14101 tgtcagcctg ttcccctcct accagctgat ctatggcgcc tttgccgcgg tgccgctgtt
    14161 cctgctgtgg atctacctgg gctggctgat cgtgctgctc ggcgccgagc tggtctgcca
    14221 tctcggcgat ccgggcagct ggcgcggtgt ggcgcgcccg ccgttgctga acctgctggg
    14281 actgctgggg cagctgttcg ctcgtcagca gggtggccgt tcgctgtcgc gtgccgcgct
    14341 ggcgcgggat ggctgggcgt tgagcgagga ggagtggctg gagctgatcg gctttctcga
    14401 gcgcgagcgc ctggtgagcg ccaccagcaa tggcgactgg gtgctgtgtc gcgacctgtc
    14461 gcagctgtcg ctggcctggt tggtggagcg ttcgccgtgg cggctgccgg cgctggccgc
    14521 cctgccggag cacttgccgg cggggctgga cgcgccctgg tatccgcgtc tgcgcgccgc
    14581 cgtgcaggcg ctggagcagg cacgtgccga ggcgctgggc ggcagcctgg ccgagtggct
    14641 gggcgaagcc cgcggccgcc gttgaggtcg ctcggctcag gccacgttgc tgatcaccgg
    14701 ctgcacgaag atctcctcga tcttcacgct ctgcggcatg aacgcactgc gcaggcgcag
    14761 ttcgacgcgg gacaggctct gcttcagcga caggcgggca acgccggaga gcaggatgcg
    14821 ctgttgcgga tcgatgcggc cgtggtcgac cgacacttca cgctcctggt cgccgtcgag
    14881 gtagctgacc agcagtgaca ccggcaggtc gctgaggccg ggcaggtgca gccaggcggc
    14941 gacttccagt tcgaccaggc ggccatccag cgcggtgctc cgcggccgtg cggcggccag
    15001 ttgctgggcg gtcagggcgt ggagcaggcg atcttggttg ggcagcatgc aggcctccgg
    15061 aagcggtaat ttggcgccag aaattgtacg aagcccgtcg gcgcggaact gtggtgcagg
    15121 accgaccggc aggtgacctg ttgcgcagtt tgcgcgggca gccgctctgc cgcgggattt
    15181 tgccggcgcc ggcggtttcg gctaagttgc atccagcaac ggatgagtgg gggtgggcag
    15241 gtgatgcgaa ttcgatggag cagcgccgtg ccgctgctgt gtgcggctct gctgctaggc
    15301 ggctgcagcg aagcggactg gggagccgat cagcacggcc gcccggtcgc ggcggcgcag
    15361 ctggacggcc gctggctggt gatcaactac tgggccgagt ggtgcggtcc ctgccgcaag
    15421 gaagtacccg agctgaaccg tctggccagc gaactcgctg cgcagccggt cagggtgctg
    15481 ggggtgaatt tcgacggctt gcagggcgag gagctgcgca aggcgaccgc ggcgctgggc
    15541 atcgcctaca ccaacctggc cagcgatccc gccgcgcggc tggggctgac cagcagcccg
    15601 gtgttgccgg tgacctggct ggtcgacccg caggggcggg tgcgtgagca gctggccggc
    15661 gagcagacgt cggcagggct gcaagcgaag ttggcggcgc tgcgcgcgca accatagcgc
    15721 tacgcgaacg gccgatgtcc gctgaggtcc cgtcggccag gcaggccttc agtcctcctg
    15781 tgggcggtcg gccgcgacgc gcccggcatc cttgagcagg gcctggatca gctcctgctg
    15841 cagttccgga tcgttgcgcg tgagctcgat caggctctgc tccagttcgc cggcttcctc
    15901 ttccaggccc agttcggcca ggcgcttgac ccgatgcacc cagtgcgcca cgtcgccttc
    15961 ttcgaggtcc tcgtagatca gcggctgggc ttcctgcagg cggctgcgca agtcgcggct
    16021 gaccggcagg gtgagctcgg tgcgggtctg gtcctgcggg tcttcgacgc gcagttgcag
    16081 cttgccgagg cggcttacgt cctgttcggc cagcgggctg tccagcaggt tcaggcgcag
    16141 gacgccgttg cggttggtgg tcagctcgta ggtctgcttg ccggcggtga ccagcaccgg
    16201 gcgctccgcc caggggaggc tggaggtttc caggcgggtt tcgccctgtt gctcgtccag
    16261 tgcggccagg ctctgctggg cgcggccgtt ggactcggcg ttcatgaacg ggttgaggcc
    16321 ggcgaagccg tagctgatcc actcgcgggt ggcggtttcc ggcaggctgc cgagcagggg
    16381 cacgttgagc acgttggcac cgaccccggc gagcaccgcc accacaccca gcgggatctc
    16441 gtagagttcg cgccagggct ggtagggcgt atagcgctgg tagcggcggg tcacctcgta
    16501 aggggtggcc tcgaaggtct tctgctccag gacgcgcaca tggcgttgcg gcagttcgag
    16561 taccggcggt gtgccggcgt cgacctgcag gctgtgctcg agcagcttgc gctcgacccg
    16621 ctcctcgagt tcgctgcgtt gcggcagctg gttggcacag ccggtgagga acacggcgcc
    16681 gcacaaggtg gcgccggcaa agcgatggca gcgtctgggc atgatctctt ttaacggttg
    16741 atgcgggcgg cgaggaaggc ctgcagctcg gcaagcggca cgctctgtgc ttcgctgtcg
    16801 cggcggccct tgtattccag aaccgactcg gccagaccgc gctcgctgac cacgatgcgg
    16861 tgcggaatgc cgagcagttc catgtcggca aatttgacgc ccggactggt cttcttgtca
    16921 cggtcgtcga acaggacttc gtagccggca gcgctcagct cggcgtacag gcggtcggcg
    16981 gcttccttga ccgcggcatt ttcgtacttc atcggcacga tggcgatctg gaacggtgcc
    17041 agcgcctccg gccagaggat gccgcgctcg tcccagttct gttcgatggc ggcggcgacc
    17101 acgcgggaca cgccgatgcc gtagcagccc atagtcaggg tgaccggctt gccgatctcg
    17161 ttgagcacgg tcagcttgag ggcctcgctg tacttggtgc cgagctggaa gatgtggccg
    17221 acctcgatgc cgcgcttgat caccagggtg ccctggccgt ccgggctcgg gtcgccctcg
    17281 accacgttgc gcaggtcggc gacttccggc agcggcaggt cgcgttccca gttgaggccg
    17341 aagtagtgct tgccgtcctg gttggcgccg gcggcgaagt cgctcactag ggccaccgag
    17401 cggtcgacga cgcaaggaat gttcaggttc agcgggccga gcgagccggc gccggcgccg
    17461 gcgccgatgg cggcgcggat ctcggcttcg gaggccatca ccagcgggct ggcgacctgg
    17521 gccaggttgg cggccttgat ctcgttgagc tcgtggtcgc cgcgcaccag cagggcgacc
    17581 agggtgcctt cctcggcgcc gcgcaccacc agggtcttga cggtcttggc gatggccacg
    17641 ccgaactggg cgaccagctc gtcgatggtc ttggcgtccg gggtgtcgac caggcgcagt
    17701 tcctcgctcg ctgcagcgcg cgcggtctcg cgcgggatgg cctcggcctt ctcgatgttg
    17761 gcggcgtagt cggagctgtc gctgaaggcg atgtcgtctt caccggactc ggcgagcacg
    17821 tggaactcgt gggagccggt accgccgatg gagccggtat cggcctgtac cgggcggaag
    17881 ttcaggccca ggcgggtgaa cacgttgcag tacgcctggt gcatgcggtc gtaggtttcc
    17941 tgcagcgatt cctggttgag gtggaaggag taggcatcct tcatcaggaa ttcgcggccg
    18001 cgcatcaggc cgaagcgcgg gcggatctcg tcgcggaact tggtctggat ctggaagaag
    18061 ttgatcggca gctgcttgta gctgttcagc tcgttgcgcg ccaggtcggt gatcacttcc
    18121 tcgtgggtcg ggccgacgca gaactcgcgg ccgtggcggt ccttgatgcg cagcagctcg
    18181 gggccgtact gctcccagcg cccggattcc tgccacagct cggccggctg aatggccggc
    18241 atcagcacct cgagggcgcc ggcggcgttc atttcctcgc gcacgatgtt ctccaccttg
    18301 cgcagcacgc gcaggcccat cggcagccag gtgtacaggc cggaggccag cttgcggatc
    18361 atcccggcgc gcagcagcag ctggtggctg atcaccacgg cgtcggtggg ggtttctttg
    18421 agggtcgaga tcagatactg gctggtgcgc atgggtgggg caatccggtt tcgcgaggcg
    18481 gaaaaatgct cgcattgtac ggcgcgggtt tggtggcgta cagggtggcc ggccgcgtcg
    18541 ggcgaggcgg acggatagcg caaagcagca gcccggcaca gggccgggct gctgctggtt
    18601 gcgatggggg cgcgcgttac aggatgctca gcgggtactc gacgatcagc cgggtttcgt
    18661 tctggtcgac gtcatagtcg gcgttgtagg cgctgttggc gcgccagacg gcatgccgca
    18721 cggtgaagct caggtccttg gcgacgccgt cctggatcac gtaggcggtt tcgaagttcc
    18781 actcgtgctc ctggccttcc tcgccggcgg cggtgctgat ctggtcgccg gtgacatagc
    18841 gggtcatgaa gctcagcccc ggaacgccat agccggccat gtccaggtcg tagcgcgcct
    18901 gccaggagcg ctcgtcttcg ccgacgaagt cggagaactg caccgagttg ccgaggtaca
    18961 ccgcgccacc gccatcgaca ccgtagtagt agccggtatc gccgctggag cgctgatggc
    19021 cgagggtgaa ggtgtgcgtg ccgagggtat aggcggcggc caggctccag atggtgttgt
    19081 cggcgtcgct ggcgtcgcga ttgtcgaagc gggtcttgta gatgttgaag tcgaagttca
    19141 gtgactgctc ttcggccagc ggcagggtgt agttggcgtt ggcgtagtgc ttcttgaagc
    19201 cgcggacatc gctgccgccc acctcgacgt cggagaagta gtaggccacg ttcagctcgt
    19261 cgctgacggc atagctggcg ccgagcaggt cgagggattg cagcttgccg acatcgtcct
    19321 cgtagctgtc gtggtgcatc tgattctgtg cggtcagcgc agtgaagtgg ccggcattca
    19381 gttcgagacc ttcgatctcg cggctggtcg ccaggaagcc ctcgacgctt tccggcagca
    19441 ggcggctgtc gtcggtggac agcaccggca ggtcgacgaa ctgctcgccg tatttcaggg
    19501 tggtgctgga gatgcgtgcc ttcactgccg cgccggcgcg ggaaaaggtg tcctcggagc
    19561 ggccgtcgct gccgggctgc atgtcgagcg caccggcgcg gcccttgccg ctgtccagct
    19621 tgacgccggc caggccgaag gcgtcgacgc cgaagccgac ggtgccctgg gtgaaaccgg
    19681 attcgtagat ggcatggaag ccgaggccgg tttcttcggc atagcccgcc ggattaacag
    19741 attcgctgct gccattgctg gcaccgtggc gggagtcgat gttcatgtac atcgcgcggc
    19801 tcaatacgga caggctactg tcctcgacca ggccctgggc ttcggactgc tggctggcca
    19861 gggccagctg ggtgctgccg gcggccacgg cgagggcgat gaggttccat ttcatgggca
    19921 tggggactcc agtaggtaag gttgcgctgt cctgtggggc aatctagtcg agcgcatcat
    19981 tattgtcatt ggtatgtaac tttataacat gtcggttggg gtgtgaaagc cccgacagcg
    20041 aacggcgtcc ggtgccgggg cgcctgcctg cgccggcgct tgtccgtgct taatattcgg
    20101 ttaagtctac cagtccacag tttcgaccat accctgcaca ggaggcctgt tatgccgcta
    20161 cttagggatg gccaaaacga ttttctagcc acggtcggca aagatgccga actggcgctg
    20221 cacatggcgc tgccggagtc ggacgccgac cgtcgccagc aactggaaca cttcatcggc
    20281 gaccgcttcg ctgcgcatta cggggcgcgg gtgcgccact tcatgccctg cctgctggga
    20341 ctctacgacg ccgaacagca gttgcaggcc gctcttggcg tgcgctgcgg gcagcaaggg
    20401 ccgctgtttc tcgagcgcta tctggatgtg ccggtcgagc agcgcatcgc tgagctcagc
    20461 ggtggcagcc agcccttgcg tggccagatc gccgaggtcg gcaacctggc ctcgctggcc
    20521 gcagggcatg cacggctgat gatcgtggcg ctgaccgacc aactggcgac cgcggggttc
    20581 cgctgggtgg ccttcaccgg cacgctgctg ctgctcaaca gcttccagcg cttgggcctg
    20641 aacctgttgc acctgggccc ggccgacggt gcgcgcatgg gcgacgagct gggcgactgg
    20701 ggcagctatt acgccaccaa cccgcaggtg atggttggcg acgtgcgggc ctgccatcag
    20761 cgcctgcaga gcatgggcat ctatcggcgg ctgggttacc agtcgcatcg cccgctggac
    20821 ggggagttcg cccatgtcgc ctgcggttga gcggtttcgc cagcaactga gctgctttgc
    20881 cgcacgcgtg gcggtcacgt gtggcgaacg ccgctggagc tatgcccagc tgctggacga
    20941 ggtggcggtg cgccaggcga tcctgcagcg cggcgctgtg cagcggctgg cgatcgccct
    21001 ggacaacggc ccggagtggc tgttctggga tctggccgcg ctgttcggcg gcgtgccctg
    21061 cgtgccgctg ccaggcttct tctcgccggc gcagcagcgc catgtgctcg acacttccgg
    21121 ggtcgacacc ctggtcgcca gtaatccgca ggcgttcgcc gcactgggct tcgtggccgc
    21181 cggcgatggg ctgtgccagc gcccggtcgc cgcgccggcg gcgctgcccg ccggcacggc
    21241 gaagatcacc tacacctccg gcaccactgg ccagcccaag ggcgtatgcc tgtcggccga
    21301 tgccatgctc agcgtggcgc agagcatcgc ccaggccacc gccagcagcg tcatcgagcg
    21361 gcacctgtgc gtgctgccgc tggcggtgct gctggagaac ctggccggca actacacgcc
    21421 gctgctgctc ggcgccagtc tggagattcc gccgctggcc gaggtcggca tgcgcggcgc
    21481 gagccagttc gagctgccgg ccttcctggc cagtctggcg cgggggcggc cgaacagcct
    21541 gatcctgctg ccgcagctgt tgctggcgct ggtcgttgcc gccgagcagg gcctggcgct
    21601 gcccgattcg ctgcgcttca tcgccgtcgg cggcggccgg gtggcgccgc agctgctcga
    21661 gcgtgccgag gcgctggggc tgccggtgtt cgaaggctac ggcctgtccg agtgttcctc
    21721 ggtggtgtgt ctgaacaccc ccgagcagcg gcgcatcggc accgtcggcc ggccgctggc
    21781 gcatgccgag gtcagcttgg cggaggatgg cgaagtactt gtgcgcggcg cgcgcctgct
    21841 cggctatctc ggcgagccgg ccccggcgga cgactggctg ccgaccggcg acttgggcca
    21901 ctgggacgac ggtttcctgg tcctgcacgg gcgcaagaag caccagttcg tcaccgcctt
    21961 tggccgcaac gtcaaccccg aatgggtcga ggccgagctg gtccagcaac tgccgatcgc
    22021 ccaggcctgg ctgcatggcg aggcgctgcc ggccaacgtc gcggtgctgg tgccgcgccg
    22081 tggcgagatc ggcgatgcgc agctgcaggc ggccgtcgac caggtcaacg ccggtttgcc
    22141 ggactacgct cgcgtgcacc actggctgcg cgccgagcaa cccttcagcg aggcgaatgg
    22201 cctggccacc gccaacggcc ggttgcgccg cgccgccctg tttcagcatt accgcagcgc
    22261 catcgacacc ctgatggccg gatcgaacgt ttaaaggaag cgcattgatg agtttctacg
    22321 agaccctgca agagcagacc gttgccgagc gcgagtacct gctggccgcg ccgatcctcc
    22381 gccaggtcct ggccggcggg atcggcctcg acagctatgt ggccttcctc ggtcaggcct
    22441 accatcacgt caagcacacc gtgccgctgc tgatggcctg cggtgcgcgc ctgccggagc
    22501 ggctggagtg gctgcgcgag gcgattgccg aatacatcga ggaggagtac ggccaccagg
    22561 agtgggtcct caacgacatc cgcgcctgcg gcgccgatgc cgaggcggtg cgccgggaga
    22621 cgcccaacct gccgaccgag ctgatggtca gctacgtcta cgaccgcatc gcccgcgaca
    22681 acccggtgag cttcttcggc atggtcaacg tgctggaagg caccagcatc gccctggcca
    22741 cccaggcggc cggcatcatc caggggcacc tgcagctgcc gccgcaggcc ttcagctacc
    22801 tgacctccca cggcagcctg gacatcggcc atgtcgagtt cttcaagaaa ctgatgaacc
    22861 gtctcgacga tccggccgat caggccgcgg tgatccacac ttccaaagtg gtctatcgtc
    22921 tgtacggcga catgttccgc agcctgccgc tggccgctgc cggggagaac gtcaatgcaa
    22981 ctgcctgagt gccgcatcct gctgactggc gccactggcg gtatcggcca gcagctggcc
    23041 gaacgcctgt gcgccggcgg ggcccacctg ctgctggtcg ggcgccaggc ggcgcgtctc
    23101 gagcaactgg tggcgcagag ccatggccgc gccgagagcg tgtgtgccga catcacctcc
    23161 agtgcgggcc gtgacgccgt gctgcaggcc gcccggcgct tcggcggcat caacgtgctg
    23221 atcaacgcag ccggcgtcaa tcacttcagc atgctcgagc agcacgacga ggaggccctg
    23281 gccgcgctga tccagctcaa cgtcaccgcc accctgcagc tcacccatcg cctgctgccg
    23341 ctgctgcgcc agcagcagcg cgcgctgctg gtgaacatcg gctcgacctt cggctccatc
    23401 ggctacccgg gcttcaccgc ctactgcgcg agcaagttcg ccctgcgcgg cttctccgag
    23461 gcgctgcgcc gcgagctggc cgacacgcgg gtcaaggtca tgtacatcgc gccacgcgcc
    23521 acccgcaccg agatgaacga gcagagcgtg gtggcgatga acaccgaact caaggtggcg
    23581 atggacgcgc cggcgctggt ggccgacgcg gtggtcgccg cgattggccg cgagagcgag
    23641 gagctgtacc tcggctggcc ggaaaagctt ttcgtgcgcc tcaacagcct gctgccgcgg
    23701 gtggtggatc aagccctgcg caagcagctg ccgatcatcc agcgctttgc ccgcagcaag
    23761 tcctgacctt tcccggagtg gagaacccgt atgccgaaat ccatcgtgcg taacctgatc
    23821 ctgggtggtg ccctgctgtt cagtctgccg agcttcgcct tgagcgacgg cggcgagcag
    23881 agcctcaagc gcctgcaggg ccgctgggcc gagatcaact accagttgcc ggagacgcag
    23941 cgcgaggcgg cgttcgccag gctggccgac gaggccgaga ccgcgctcaa ggccgagccg
    24001 caggccgccg agctgctggt ctggcgcggc atcatcctca gcacctgggc cggcgccaag
    24061 ggcggcctgg gtgcgctgga tctggtcaag caggcgcgcg gccagttcga ggcggcgatc
    24121 aagctcgacg ccagggtcct cgacggctcg gcctacacca gcctcggctc gctgtactac
    24181 caggtgccgg gctggccgat cagcttcggc gacgacaagc gcgccgagca gctgctcaag
    24241 caggcgctca gcctgaatcc ggacggcatc gacgccaact atttctacgg cgacttcctg
    24301 ttgcgccaga aacgccatgc cgaggccaag gtggcgctgg agaaggcgct ggctgcgccg
    24361 ccgcgcccgg gccgcgaggt ggccgatgcc gggcggcgca aggaggcgca ggcgctgctg
    24421 cagaaggtgc aggccgcgca ggactgaggc gagggcaaac cctggggtga cgggctgagc
    24481 ccgtcgtctg cgcgccgggc tggcgagagt gcgatactcc cgtcagtccg gcgcagtcat
    24541 gtccgaggca tgcagaccag ggtggccggg atgggctgac agaggagggg tagatgcgga
    24601 tattgctggt ggaagacgac aaggcgctgg gcgagggcat ccgcaccgcg ctcaagcccg
    24661 agggctacac cgtggactgg gtgcaggacg gcgtcagcgc cctgcatgcc ctgagtcacg
    24721 agagcttcga gctgtgcgtg ctggatctcg gtctgccgcg catggacggt ctggaggtgc
    24781 tgcgtcgcct gcgcacgacc ggcaatgccc tgccggtgct ggtgctgacc gcgcgcgatg
    24841 ccaccactga ccgcatcgcc gggctggatg ccggcgccga tgactacctg accaagccct
    24901 tcgacgtcgc cgagctcaag gcgcgcctgc gtgccctgct gcgccgcagc ttcggtcgcg
    24961 cccagtcggc gctggaatac cgcggcatcc gtctggagcc ggacagccag caggtgacct
    25021 accagggcgc gccggtcaac ctgccgcgca aggagttcct cctgctccac gaactgctgg
    25081 cccagccggg gcgcgtgctg acccgcgaca agctgcagca ggtgttgtat ggctggaacg
    25141 aggaggtgga aagcaacgcc ctcgaggtgc atgtgcatca cctgcgcaag aagttctttc
    25201 ccgaactgat ccgcacggtg cgtggcgtcg gctacctggt ggacaaggcc tgatgctgcg
    25261 ctcgatccgt tcgcgcaccc tggggttggt gctgggtgtg ctgtgcgtcg ccctgagcct
    25321 gatttcctgg cgcagctacg tcgatgccca gcacgagatt gaggagctgt tcgatgccct
    25381 gctggcacac agcgcccgcc tgctcgaagg catggcgggc ggcgatctgt cgccgtccgc
    25441 ccgggcggcc ctgcagcagg ctctggatgc cgcattgcgt gccggacggg gcgcggaagc
    25501 ccggctcgac ggccatccct acgagagcaa gctggtgttc caggtgctcg accagcaggg
    25561 gcgggtgcag gtgcagtcgg ccggtgcgcc gacgcagttg ctggcccgcc tgctggcgca
    25621 attgcaggcc gcggagcggc tggcgctccc cagcggccag ccgctggccg cggtggcgca
    25681 gcaactgcgc ggctaccacg acgtccgtct ggagggccgc gtctggcgcg tgttcctgcg
    25741 tcaggaccag gccgaccgtc actggctgct ggtggccgag agccaggatg tgcgcgacga
    25801 actggtcggc aagatcgtcc tgcgcagcct gctgccggac ctggtcggcc tgccgctgct
    25861 ggcgctgctg gtgtggtcgg cgattggttt gggcctgcgc ccgctggagc aggtggtgcg
    25921 cctgatcaag gcgcgggatg cggaaaatct cgcgccgctc accctggcgc ccctgccgcg
    25981 ggagctggag ccgatggtcg ccgcgctcaa tcgcctgctg ctgcaggtca ccagcgtgct
    26041 ggaacgggag aagcgcttcc tcgccgatgc cgcccacgag ttgcgcacgc cgctggcggt
    26101 gctgcgcatc cacgcgcaga acgccctgga ggcgccagag gccgccgacc gcgccgcggc
    26161 actgcagcag ctggaaggcg gtgtcgaccg tgccacccgc gtggtaaccc agatgctcac
    26221 cctggcgcgt cttgccccgg atgccctgca gctggccatg gccgagctgg atctggcgcc
    26281 ctacgtgcgc gcggaactgg ccgagctgat ccccctggcg ctggagcgcg agcaggagct
    26341 ggtcctcgac tacgacgaga ccgcggatta ccggctggtg gccgacgcgc cgagtctggg
    26401 caccctgctg cagaatctgg tcggcaatgc cgtgcagtac accccgcccg gtgggcagat
    26461 cgccgtccgc ctgcaggccg aggcgcagcg ggtgggcctg cgcgtcgccg acagcgggcc
    26521 cggggtgccg gccgagcagc gcgcgcagct gttccgccgc ttctaccggc agggcgaagg
    26581 gcggggcgcg gggctggggc tgtccatcgt ccagcgcatc gttgaactgc atggcggcga
    26641 gatcctgctc gaccaggcgg cgctgggtgg cctcgaggtg atcgtgtggt tgccgcgcaa
    26701 tctgccgggc ggccgttgag cggcaagctg tggcacactg aaaacaccgc cggcctgcgc
    26761 tgatcgcgtg ctgcggcacg tcgccggtca cgccagcgcc gggcaaccct cagctgttca
    26821 acaaggagat gccgccatgt tcgccctcga tccccgcctg cagcaggaca ccctgcatct
    26881 gggcgacttc ccgctgtgcc gcctgctgct gatgaacgat gccagctatc cctggttcat
    26941 cctggtgccg cgacgcgagg aggtcagcga gctgttccag ctcgacgagg ccgatcagcg
    27001 ctcgctgtgg cgcgagacca ccctgttggc cgcgctgctc aaggacgtct tcaaggccga
    27061 caagatgaac gtcgccaccc tcggcaacgt ggtcagccag ctgcacatgc acgtcatcgt
    27121 ccgccggcgc agcgacgcgg cctggccggc gccggtgtgg gggcgtccgc cggcggtgcc
    27181 ttacaccgag gacggggtga aggaggtgcg tgaccggctg aagctggtcc tggacagcgc
    27241 cgacttccgt ttcgcggcgg aggcggcatg agcctggagc tgcgcgttac cgagctggaa
    27301 acccgcctgg cgttccagga cgatgccctg caacagctga gtgacgagct gctggcgcag
    27361 aaccggctga tcgagcgcct gcagcggcag atcgaggcgc tggcctcgcg ccaggccgat
    27421 ctggccgggc agatcggccc ggtggaggac gaggcgccgc cgccgcacta ctgaattcgt
    27481 gcccggaaac gacagagccc gccaaatggc gggctctgtc gttttcgctc cactagatgc
    27541 ggatcagtcg aggatcagca catcctcggc ctgcaggccc ttgtcgcgct gggccacggt
    27601 gaactccaca cgctggccct cgaccagcac ccgatggcca tcgccgcgaa tggcgcgaaa
    27661 gtgcacgaag atgtcgtcgc cgctgtcgcg ggagatgaag ccgaagccct tggaggtgtt
    27721 gaaccacttc accgtgccgg tttcgcgacc tgcgctcggc gcgctgcggc ggacgctggg
    27781 cgcgggcttg gcatccgcgg cggggctgac ccgaacggcc ggctgctcgg tcggtgccgt
    27841 ggcttgcgcc tgcccggccg gctggcccgg cagcagttgc aggaaggcag caatcgcgac
    27901 ggcgagcagg gtgtacagca ggaccggctg gccggccagg ttgtccagcg gcagcagcag
    27961 ggcgatgccc tgcagcactg cggcgagcac cagcagggcg ctgctcagcg gttgcacgcg
    28021 ctggcgggag gcgccgacca gcggggagat cagcaggttg gtcaggccga tgaaggccag
    28081 gtacagggca tcgggttgtt gcagccaggg cagggcttcg ccgcgcaggc tgggaacgaa
    28141 agacagcagc agggcagcca cgcccgacaa cagatggacg gttttcaata tgctcaacga
    28201 attcaccttg gtaacgaaca aatactggac agaggatgtc cgggcagacg gggtcgtgct
    28261 gaaaggaaag gacaccagat gatggcgtac cggtcggccg gacggcgcct ggccgggctc
    28321 gacgcgaatg ggctcgagcg gattaggcgc ggcggctatt taaccgtaaa ggcctcgtac
    28381 tctcaattgc cccgtgtacg gcgcgccgcg gaatcggctg ctgccggggc aggcgcgggg
    28441 ggattatttc aggctataat cccggccctt tatctcggca aggcctgctg ctgcccgcgt
    28501 gtttgcggca ggctggcggc tgtggcggca gcggttgcct ggcagccgcg gatcgaccgc
    28561 cgcgcggaat ttacgactga ctttgagaag cgaaccgacc atgatgcgca gccactattg
    28621 cggccaactc aacgaaagcc tggacggcca ggaagtcacc ctgtgcggct gggtccatcg
    28681 ccgtcgcgac cacggcgggg tgatcttcct cgacatccgc gaccgtgaag gtctggccca
    28741 ggtggtgttc gacccggatc gcgccgacac cttcgccgcc gccgaccgcg tgcgcagcga
    28801 atacgtggtg aaaatcaccg gcaaggtgcg tctgcgcccg gccggtgcgg tcaacccgaa
    28861 catggccagc ggcgccatcg aggtgctcgg ctacgagctg gaagtgctca acgaggcgga
    28921 aaccccgccg ttcccgctca acgaatacac cgatgtcggc gaggaaaccc gcctgcgcta
    28981 ccgcttcatc gacctgcgtc gcccggaaat ggccgagaag ctcaagctgc gctcgagcat
    29041 caccagcagc atccgccgct acctggacga taacggcttc ctcgacgtcg agacgccgat
    29101 cctcacccgc gccactccgg aaggcgcgcg tgactacctg gtgccgagcc gcacccacgc
    29161 cggcagcttc ttcgccctgc cgcagtcgcc gcagctgttc aagcagctgc tgatggtcgc
    29221 cggcttcgac cgctactacc agatcgccaa gtgcttccgc gacgaagacc tgcgtgccga
    29281 ccgtcagccg gaattcaccc agatcgacat cgagaccagc ttccttgacg aagacgacat
    29341 catcggtctg accgagaaga tgatccgcca gctgttcaag gaagtgctgg atctggagtt
    29401 cggcgaattc ccgcacatga cctacgccga ggccatgcgc cgctacggct cggacaagcc
    29461 ggacctgcgc atcccgctgg aactggtcga tgtcgaagac cagctcaagg atgtcgactt
    29521 caaggtgttc gccggcccgg ccaacgatcc gaagtgccgc gtgaccgccc tgcgcgtgcc
    29581 cggcgcggcg agcatgccgc gcaagcagat cgacgactac accaagttcg tcagcatcta
    29641 cggcgccaag ggcctggcct acatcaaggt caatgagcgc gccaagggcg tcgaaggtct
    29701 gcagtcgccg atcgtcaaga acatcccgct cgacaatctc aatgtcatcc tcgatcgcgt
    29761 tggcgccgtc gacggcgaca tcgtgttctt cggcgccgac aaggccaaga tcgtttccga
    29821 ggccctcggt gccctgcgca tcaaggtcgg tcacgacctc aacctgctga cctgccagtg
    29881 ggcgccgatg tgggtggtcg acttcccgat gttcgaggaa aacgacgacg gctcgttcac
    29941 cgcgctgcac cacccgttca ccgcgcccaa gtgctcgccg caggagctgg aagccaaccc
    30001 gggtaccgcc ctgtcgcgcg cctacgacat ggtgctcaac ggcaccgagc tgggtggcgg
    30061 ttcgatccgt atccaccgca aggaaatgca ggaggcggtg ttccgcatcc tcggcatcga
    30121 cgaggccgag cagcaggaga agttcggctt cctgctcgac gccctgaagt tcggcgcacc
    30181 gccgcacggt ggcctggcct tcggtctgga ccgtctggtc atgctgatga ccggcgccag
    30241 ctcgatccgc gaggtcatcg ccttcccgaa aacccagagc gccgcctgcg tcatgaccca
    30301 ggcgccgggc atcgtcgaca acaaggcgct gcgcgaattg cacattcgcc tgcgcgaaca
    30361 gcccaaggcc gaataagccg acacaggagc ctggtttgcc aggctccttc gttagagccg
    30421 gtcacaatct gctgcgcgtc ggccatgctg cgttgaaatc ggtttcggaa tgctcatttg
    30481 cagtcagcaa actccgcttc ctcaaccgat ttcgccttgc ctggctctag ctcgccagat
    30541 tgtgagtcgg ctctgcgggg cggtaaaagt acaagatcca ccagaaatac gacggagaaa
    30601 gtcatggctg gtcattccaa atgggccaac atcaagcacc gcaagggacg ccaggacgcc
    30661 aagcgcggca agatcttcac caagctgatt cgtgagctga ccgtttccgc caagggtggg
    30721 gcgagcccgg ctgacaaccc gcgcctgcgc ctggcggtgg acaaggcgct gaccgccaac
    30781 atgtcgcgcg acgtgatcga ccgcgccatc gcccgcggtg ccggcaccaa cgaagccgac
    30841 aacatggccg agctgagcta cgaaggctac gcgccgagtg gcgtggcgat catcatcgag
    30901 gcgatgaccg acaaccgcaa ccgcaccgcc gccgaagtgc gccatgcctt cagcaagtgc
    30961 ggcggcaacc tgggcaccga cggctcggtg gcctacatgt tcgaccgcaa ggggcagatc
    31021 agcttcgccc cgggcgtcaa cgaggaggcc ctgatggagg ccgcgctgga agccggtgcc
    31081 gacgacatcg tcagcaacga cgacggctcg gtggatgtgt tcaccagctt cgccgacttc
    31141 catgcggtca acgaggcgct caacgccgcc ggcttcaagg gcgacgaggc cgaggtggcg
    31201 atgatcccgt cgatcagtgc gccggtcgcc gacctggaga ccgcgcagaa ggtgctcaag
    31261 ctgatcgaca tgctggaaga cctcgacgac gtgcagagcg tctatcacaa cgccgagatc
    31321 ctcgacgaga tcatggagca gctggactga ggtccgggcg cctgcgtggc ctgagccgga
    31381 aggggcgcgc gccccttccg gcttttcttt tgggaaggcg gtcatgcggc ggtgtgccgg
    31441 gcgtgacgcc gccgcagggg ccacctatac tcgcggctgg ataaatagac agtgaggccg
    31501 gcgcggcgtc attgtccgac acgcttggat gacgcctgca tgaccctgat cctcggtatc
    31561 gaccccggct cgcgcatcac cggctacggc gtggtgcgcg ataccgggcg tggctgcgag
    31621 tatgtcgcct ccggctgcat ccgcaccggc gacggcgcgc tgcccgagcg gctgcagaag
    31681 gtctatcgcg gcgtgcgcga ggtgatccgc ctgcacgggc cggtgatgct gggcatcgag
    31741 caggtgttca tgtcgcgcaa tgccgactcg gcgctcaagc tcggtcaggc gcgcggcgcg
    31801 gcgctggtcg cggcgatgga ggaggggctg agcgtggccg agtacaccgc cacccaggtc
    31861 aagcaggcca tcgtcggcac cggcgcggcg gacaagcagc aggtgcagct gatggtcatg
    31921 catctgctca agctggtgca gaaaccgcag atcgacgcct ccgacgccct ggccatcgcc
    31981 ctctgccatg cccatcatcg gcagagtctg ctcccccatg gcctgtccag cgcgcgtcgg
    32041 cgcagcggac ggctgcgttt gtaacaagga aaccggatcg tgatcggacg tttgcgcgga
    32101 accctggcgg agaagcagcc gccgcatgtg ctgctcgagg tcggcggagt cggctacgag
    32161 ctggaagtgc cgatgagcac cctgtaccgc ctgccgggcg tgggcgagcc gctgaccctg
    32221 cacacccatc tggtggtgcg cgaggatgcc catctgctct acggctttgc cgaaaagcgc
    32281 gagcgcgagc tgttccgcga gctgatccgc ctcaacggcg tcggtcccaa gctggccctg
    32341 gcgctgatgt ccggactgga agtggacgag ctggtacgct gcgtgcaaac cggcgacacc
    32401 aaggccctga cccgggtgcc gggcgtcggc aagaaaaccg ccgagcgcct gctggtggaa
    32461 ctcaaggacc gctttcaggt ctgggaatcc atgccgggtg cgccgctggt ggtggagccg
    32521 gcgcgcgcgg ccagcgtctc cagcgccgag gccgatgcgg tcagcgccct ggtcgcgctc
    32581 ggcttcaagc cggcggaagc cagccgtgcc gtcgccctgg tggacgaaga cggtctgtcc
    32641 agcgaagaat tgatccgtcg tgccctcaag ggcatggcct gaacccgtgg ataagccccg
    32701 atgattgaag ccgaccgcct ggtcaccgcc agcccgcgcg agcgcgagga gcagcaggac
    32761 cgggccatcc gcccgctgaa actggccgag tacatcggcc agccggtggt gcgcgagcag
    32821 atggagctgt tcatccaggc cgcgcgcggg cgcagcgagg cgctcgatca cacgctgatc
    32881 ttcggcccgc cggggctggg caagaccacc ctggccaaca tcatcgccca ggaaatgggc
    32941 gtgtcgatca agagcacctc cggcccggtg ctggagcgcc ccggtgacct ggcggcgctg
    33001 ctgaccaatc tcgagccggg cgacgtgctg ttcgtcgacg agatccatcg tctgtcgccc
    33061 atcgtcgagg aggtgctgta cccggcgatg gaggactacc agctcgacat catgatcggc
    33121 gaggggccgg cggcgcgttc gatcaagctc gacctgccgc ccttcaccct gatcggcgcc
    33181 accacccgtg ccggcatgct gaccaacccg ctgcgcgacc gcttcggcat cgtccagcgc
    33241 ctggagttct acagcgtcgc cgacctggcc accatcgtca gccgctcggc cggcattctc
    33301 ggcctgccca tcgatgccga gggcgccttc gagatcgccc gtcgcgcccg tggcacgccg
    33361 cgcatcgcca accgcctgct gcgccgggtg cgcgacttcg ccgaggtgcg cgggcgcggc
    33421 gagatcagtc tcagcatcgc cgaccgggcg ctggacatgc tggatatcga tgcccatggc
    33481 ttcgatcacc aggaccgccg gctgctgctg accatgatcg acaagttcga cggtgggccg
    33541 gtcggcatcg acagcctggc ggcggcgatc agcgaggagc gccacaccat cgaggacgtg
    33601 ctcgagccct acctgatcca gcagggctac atcatgcgca cgccgcgcgg ccgggtggtg
    33661 acccggcatg cctacctgca cttcggcctc aacgtgccgg ggcggctggg cgagacggtc
    33721 gctgccggcc aagccggcgc cgatgattga cggaaaaatg gttggcaagc cagggagcca
    33781 gtactagagt atgcgcgcgc aaaacacggg tctgtcgttc acccatcgct gtcgggtcta
    33841 ctatgaggac accgacgccg gtggcgtcgt ctattacgtc aactatctca agttcatgga
    33901 gcgggctcgc accgaacggt tgcgtgcgct gggcttcgtc cagtcgcaac tggcggggga
    33961 gaacctgctg ttcgtcgtgc attcggccga agcgcgctac ctggcgccgg cccggctgga
    34021 tgatgaactg ctggtcagtg ccgaggtagt ggagctcaat cgtgccagcc tgcgctttca
    34081 ccagcaggta cggcgggccg gcgatgacgc gctgctctgt gaaggacgag tcacggtggc
    34141 ctgtgtgcgc gccgacagtt tcaaaccccg ggccattccc gaagcgctgc gagcagcctt
    34201 cagcggcgcg agtccaatcc ctgcaggaga gtaagcgtgg aagccaacgc cgttgaccat
    34261 atgtcgatgt ggagcctgat cagcaacgcc agtttcgtgg tgcaactggt gatgctgatc
    34321 ctggtggccg cctcggtcac ctcgtggatc atgatcttcc agcgcagcag tctgctgcgc
    34381 gccgccaaga agggcctgga cagcttcgag gagcgcttct ggtcgggtat cgacctgtcc
    34441 aagttgtacc gccaggccgg cagcaatccc gatccggact cgggggtcga gcagatcttc
    34501 cgggccggct tcaaggagtt ctcccgcctg cgccagcagc cgggcgtcga cccggatgcg
    34561 gtgatggacg gcgtcaaccg cgccatgcgc gtggccatct cccgcgagga agagaagctc
    34621 gaacagagcc tgccgttcct cgccacggtc ggctccacca gcccctacat cggtctgttc
    34681 ggtaccgtgt ggggcatcat gaactccttc cgtggcctgg ctcaggtgca gcaggcgacc
    34741 ctcgccaccg tggccccggg catcgccgag gcactgatcg ccaccgcgat cggcctgttt
    34801 gccgcgattc cggcggtgat tgcctacaac cgtttttcct cccgtggcga gatgctgatc
    34861 aaccgctact acaccttcgc cgatgaattc caggccatcc tgcatcgcaa ggtccacacc
    34921 agcgatgaat gatgcgctcc ccggcgtatc cgtccccgtg ctccgcgcct gcgcggcggg
    34981 gcgagggggg cagatgccgg gcaagggtct cttggatagg ttctattaag tcatggccag
    35041 aattcgcaac agacgcaagc cggtcgccga gatgaacgtc gtgccctaca tcgacgtgat
    35101 gctggtgctg ctggtgatct tcatggtgac tgccccgatg ctcaaccagg gggtcaaggt
    35161 cgacctgccc aaggtgagca gcgaggcgtt gccgcaggac aacaacgccc aggtgctgac
    35221 catctcgatc aaggccgaca agacctacta ctggaacatg ggcagcgagg tcgatgtcga
    35281 taccgcacag gacaaggcgc tgaccctcga tgagatggtg cgcgcggtca ccgcgatcat
    35341 gaaccagagc cgcagccagg gtaagcaggt ccaggtgttc gtgcgcggcg acaaggcggt
    35401 ggactacgga gcggtcatgg cggccatggg cggtctgcag caggccggtg tgggcaacgt
    35461 cgggctgatt accgaggcgc cctgatgcag cagcacgagc gttccccctc cgcatcggaa
    35521 agctatttct ggcccaccat ctgggcggta gccctgcacg ccctgatctt cgccatgctg
    35581 ttcgtcagtt ttgccatgac gccggagctg ccgccgtcgc ggccgatcgt ccaggccacc
    35641 ctgtaccagc tcaagtcgca gagccaggcc acggtgcaga ccaaccagcg catcgcgggc
    35701 gaggcgaaga aaacggcggc gccgcagtac gaaactgaac agatggagca gaagaaggtc
    35761 gaacagcagc gggtcgcggc ggccaaggcg gcggagcaga agaaggccga gcaggctgcc
    35821 gcggcgcaga aggaagcggc agcggccaag gccgaggcgc agaagcaggc ggaggcggca
    35881 gccaaggccg aggcggcgaa gaaggccgcc gagcagaagc agcaggctga tttcgccaag
    35941 aaaaaagccg ccgaggacgc caagaaaaag gccgaagagg ctgccaagca gaaagatgcc
    36001 gaagaggcgg cgaagaagaa gtcggccgag gaagccaaga aaaaggctgc cgaggacgcg
    36061 aagaagaaag ccgccgagga ggccaagaaa aaggccgccg cggaggctgc caagaagaag
    36121 gcggccgagc agtctgcccg caaggcccag gaagacaaga aggctgcggc gctggccgaa
    36181 ctgctctccg acgacgtgca acaccagcag gcgctggccg acgaggtcgg cgaccaggtg
    36241 gccggcagcc tcgacgacct gatcgtcaac ctggtcagcc agcagtggcg ccggccgccg
    36301 tcggcgcgca atggcatgag cgtggaagtg ctgatccaga tgctgcccga cggcaccatc
    36361 accaacgcca gcgtctcccg cgccagcggc gacgcggcct tcgacaattc ggccgtggcg
    36421 gcggtacgca atgtcggccg catcccggaa atgcagcagc tggaccgcgc gaccttcgac
    36481 aagatgtatc gtcagcgtcg catgatattc aaaccggagg atttgagcct gtgaacaccc
    36541 tgattcgcat ggccctgctg ggcctggtgc tgctcgccgg tatggcgaag gcagccgacc
    36601 cgctggtgat ttccagcggt accgatcggg ccatcccgat cgccgtggtg ccgttcggct
    36661 cgcagggcgg tgccgtgctg cccgaggaca tggccgatat catcggcaag gacctgcgca
    36721 actccggcta cttcgagccg attccgcgcc agaacatgat cagcctgccc agtcaggcca
    36781 gcgaggtcat ctaccgcgac tggaaggccc tcggcgccca gtacgtgctg acgggcagca
    36841 tggtgcccag cggcggcggc gtgcagatcc agtacgcgct gttcaacgtc accaccgagc
    36901 agcagatgct gaccggcagc gtcggcggca ccaccgcgca gctgcgcgac atggcgcact
    36961 tcattgccga ccagtcgttc gagaagctca ccggcatcaa gggggccttc tccaccaagc
    37021 tgctattcgt caccgccgag cgctttgccg ccaacaatac ccgctacacc ctgcaacgtt
    37081 ccgactatga cggcgcccgt gcggtgaccc tgctgcagtc gcgcgagccg atcctgtcgc
    37141 cgagcttcgc gccggacggt cggcgcatcg cctacgtgtc cttcgagcag cagcgcccgc
    37201 gcattttcgt gcagaacatc gataccggcc gccgcgagca gatcaccaat ttcgagggcc
    37261 tcaacggcgc tccggcctgg tcgccggacg gcagtcgcct ggccttcgtg ctgtcgcgcg
    37321 acggcaaccc ggagatctac gtgatggaca tgggcagccg ccaggtgcgt cgcgtgacca
    37381 acgaccagtc catcgacacc gagcccttct ggggcaagga cggccagacc atctacttca
    37441 cctcggatcg tggtggcaag ccgcagatct acaagacccg cgtcggcggc ggtggggccg
    37501 agcgcgtgac cttcgtcggc aactacaacg ccaacccgaa gttgtcggcg gatgagcgca
    37561 ccctggtcat ggtgcatcgg caggatggct tcaccgtctt caaggtggct gcgctggacc
    37621 tgcagcgcgg tggcgctccg cgcatcctct ccggggagac cagtctggac gagtcgccca
    37681 ctgtcgcgcc caacggcacc atgctaatct acgccacccg tcagcagggc cgtggagtgc
    37741 tgatgctcgt ctccgtcaat ggacgtgtgc gccttccgat acctgcggct caaggcgaaa
    37801 tccgtgagcc ttcctggtct ccctatttga actgacggcg gctgccaact gctcgtttca
    37861 ataccttgtt ttgaacacgt tgttttgaat acacactggg gttttaaaag atggaaatgc
    37921 tgaaattcgg caagtttgct gcgctggctc tggcgatggc tgttgctgta ggttgctcct
    37981 ccaagggtgg cgatgccgct ggcgaaggcg cagtcgatcc gaacgctggc tacggtgcca
    38041 acgccggtgc cgtcgacggc agcctgggtg gcgaagaagc cgctctgcgc gccatcacca
    38101 ccttcttctt cgagtacgac agctccgacc tgaagccgga agccatgcgc gctctggacg
    38161 ttcacgccaa ggacctgcag gccagcggcc agcgcgtagt cctggaaggc cacgccgacg
    38221 agcgcggtac ccgcgagtac aacatggctc tgggcgagcg tcgcgccaag gccgttcagc
    38281 gctacctggt gctgcagggc gtgtccccgg ctcagctgga gctggtgtcc tacggtgaag
    38341 agcgtgcgct ggttgccggc agcagcgaag aaagctgggc gcagaaccgt cgcgtcgaac
    38401 tgaagaagta atacgccatg cacaagcact cccgtctcct gaccctgctg gctctggccg
    38461 caccactcac gacctgggcg caggtgcccg tcatggatgg tagcaacggt tatccgccgt
    38521 ccggctacgg tgcgacggga gctgcttatg ccggcggcgc ggctaccgcg cctgcctcgg
    38581 cacagggtga actcttcctg cagctgcagc agttgcagga agaggtctcc cgcatgcggg
    38641 gcacgttgga agaacagcag tacgagatca agcagctcaa gcaggaaagt ctgcagcgtt
    38701 accaggacct ggacagtcgt ctgtccggtg caggccaggc tccggcaccc gcagcccagg
    38761 cgcccctggc tcccgcggcg gcagactcgg catctgccgg tcaggctccg gcggcgaatg
    38821 ccgcgccggg cgatccggcc aaggaaaagc tgttctacga tgccgccttc gacctgatca
    38881 aggcgaagga ttttgccaag gccgaccagg ccttcagcgc atttctgcgc aagtatccga
    38941 ccagtcagta cgccggcaac gcgcagtact ggctgggtga agtgaagctc gcccagggcg
    39001 atctgcaggg cgccgggcag tcgttcgccc gcgtcagcca gggctacccg gaccaccaga
    39061 aggtggccga ctcgctgtac aagctggccg atgtcgagca gcgtctcggc aatgtcgcca
    39121 aggccaaggg catcctgcag caggtgatcg cccagtatcc gggcacctcc gccgctcagc
    39181 tggccaagcg ggatctcgcc cgtctgccct gaacagcgag cgcttctgag gaaacccgcg
    39241 cccggcgcgg gttttttcgt taaaatcgcc gcactttttc cacatcctgc aacggaggcg
    39301 gttggcctgt ttcgccatca cgcccggggc ttatgaacca gactctgcgc atcaccgaaa
    39361 tcttctattc gctgcagggg gaagcacgta ccgtcggcct gccgaccgta ttcgtgcgcc
    39421 tgaccggctg tcccctgcgc tgtcagtact gcgataccgc ctacgccttc agcggcggcg
    39481 agatcatgac tctggagcag atcctcgcgc gggtcgccag ctaccagccg cgctacgtct
    39541 gcgtgaccgg cggcgagccg ctggcgcagc cgaactgcat cccgctgctc gcggcgctgt
    39601 gcgacgccgg ctacgaggtg tcgctggaaa ccagcggcgc cctggatatc gctgcggtgg
    39661 acacccgcgt cagccgggtg gtcgacctga agacgccggg ctcggccgag gtcggccgca
    39721 accgctacga gaacatggcg cagctgaccc gcaacgacca ggtcaagttc gtcatctgtt
    39781 cgcgcgagga ctacgactgg gcggtgtcga aaatcatcga ataccgcctg gagcagcgtg
    39841 cgggcgaggt gctgatgtcg cccagccatc atcagctgga ggcgcgcagc ctggccggct
    39901 ggatcatcgc cgacaacctg ccggtgcgcc tgcaactgca actgcacaaa tatctgtgga
    39961 acgacgagcc ggggcattga gatgagcgag aagagggcgg taatcctgct ttccggcggc
    40021 ctcgactccg ccaccgtact ggccatggca cgcgccgagg gctatgcctg ctacaccatg
    40081 agcttcgact acggccagcg gcaccgcgcc gagctgcagg cggccgagcg ggtggcgcat
    40141 cagctcggtg cgctggagca caaggtggtc ggcatcaacc tcaacggcat tggcggctcg
    40201 gcgctgaccg acagcaccat cgccgtgccg gaggagccgg ctgagggcat cccggtaacc
    40261 tacgtgccgg cgcgcaatac gctgttcctg tccctggcgc tgggctgggc cgaggtgctg
    40321 ggggcgcacg acatcttcat cggcgtcaat gccgtggact attcgggcta tcccgactgc
    40381 cggccggaat tcatcgatgc cttcgagcgc ctggccaatc tggcgaccaa ggccggcgtg
    40441 gaaggcgagg gcttccgcat tcgggcgccg ctgcagtacc tgtccaaggc gcagatcatc
    40501 caggctgggc tggcacgcgg cgtggagtat ggcttgaccg tgtcctgcta ccaggccgat
    40561 gcggatggcc gcgcctgcgg gcgctgcgac agctgccgcc tgcgtgccga aggcttccgt
    40621 gcggccggga tggtcgaccc gacccgctat ttttgaaatt ctttcccttg gggtgttgta
    40681 tttccgttag aaatcagtaa tatacatctc aagttggggc gttagctcag tggtagagca
    40741 gttggctttt aaccaattgg tcgtaggttc gaatcccaca cgccccacca tctacttctt
    40801 cctcggaaga acgaaaagct ggaagttgat ccgcaagggt tggtttccgg cttttttcgt
    40861 atgcgcagta tgcctccgtc gactctgcct gcatgcggca ggggcgcgag tctatacttg
    40921 tatcccgcac cgccgtgtca gcaggtcctg cgaatatgtc ccaagtttcc gaacgtcttc
    40981 tggttcaagc tcatctggat gccaagcagc cgcgcccgct gactgccgag gaagttgctg
    41041 actaccgcca ggcgatcgcc gccgagctca aggcgcgtga cgccgttctg gttgcccact
    41101 actacaccga tccgattctc caggcgctgg ccgaggaaac cggtggctgc gtcgccgact
    41161 cgctggaaat ggcccgtttc ggcagccagc acccggcctc gaccatcgtg gtggctgggg
    41221 tcaggttcat gggcgagacg gcgaagatcc tcaatccgga aaagcgcatc ctgatgccga
    41281 ccctggaggc gacctgttcg ctggatctgg gctgcccggt cgaggagttt tccgcctact
    41341 gcgaccagca ccccgagcgc accgtggtgg tctacgccaa cacctcggcg gcggtgaagg
    41401 cgcgtgccga ctgggtggtg acctccagct gcgcgctgga gatcgtcgag agcctgatgg
    41461 acaacggcga gaagatcctc tgggcgccgg atcagcacct ggggcgctac atccagcgcg
    41521 agaccggtgc ggacatgctg ctctgggagg gggcgtgcat cgtccacgag gagttcaagg
    41581 cccggcagct gcacgacctc aaggcgctgt atccggatgc ggcggtgctg gtgcaccccg
    41641 agtcgccgga ggcggtggtc gcgctggcgg atgcggtggg ctccaccagc cagctgatca
    41701 aggcggcgca gaccctgccg aaccagatct tcatcgtcgc caccgatcgc ggcatcctct
    41761 acaagatgca gcagctgtgc cccggcaaat cgttcatcga ggcgccgacc gccggccagg
    41821 gtgcggcctg ccgcagttgc gcgcactgcc cgtggatggc gatgaacacc ctggagcgca
    41881 ccctgcagtg cctgcgcacc ggcagcaacg agatccacgt cgatccggcg ctgatcccca
    41941 aggcggtcaa gccgctcaag cgcatgctcg acttcaccca ggcagcgcgg ctcaagctga
    42001 gcggcaacgc ctgaggggta ggcgaaaaca ccaaggcccg gctaattgcc gggccttctg
    42061 ctttacgggc tggctcagcg cagcagcttg tcgatcatcc gctgttcttc ttccagggtg
    42121 ctgtggcgag cgtcgatgcg tgcagccagc tggaagttgc tgctggcgcg gcggcgggcg
    42181 aagtcgagtt gctccagggc ctggtcgtag tcgccggtga gggcgaagta ttcggcgcgg
    42241 gcctggtgca cgccgatgat gttgccggac aggccgcggg tttcggcctg ctgataccac
    42301 agctcggcat cgccggggcg ctccttgagc agggcgtcca ggcgcttttc ggcttcggcc
    42361 ggacgattct gcttgagcag tacttcggcc tggtactggc gcagcgggta gttgtccggg
    42421 tacaggccga gcaggcgctg caggcgctgg ctggcggcat cgggccggtt ggcggcgacg
    42481 tccagctggg cctgggcgag gttgtagacg atatcgttcg gcgccttggc gagcagcggc
    42541 tgcaggctgc tggcggcctc cttgagctgg ccgttcttga tctgcgccag ggtcaggccg
    42601 tagcgcgcgg catcgaggtt ggggttttcg tcgagcaggg cgcggaagcg tttggccgcc
    42661 aggcctggtg agctttcgta gagcaactgc acgcgggcgc gcatcagttg gtagttcagg
    42721 gtgtcggtgt tgccgccgcc ggcgagttgc tcggcgcggt tgcgcgtatc ggccacccgc
    42781 gattcgctga ccgggtgggt caggagaaat tccggcggca tccgggtgta gcggtactgg
    42841 cgcatcaggc gctcgaacat gctcggcatg gcattcgggt cgtaacctgc ctgctgcagg
    42901 ttgaggatgc cgatgcggtc ggcctcctgt tcgttctgcc gcgagaagct ccgttgtgcc
    42961 tggaccgccg ctgcctgggt cgaggcgatg gcggcgatgc cggcatcgcc ggcgccggcg
    43021 gcggcggcga ccacgccggc gagcatggcg gccatcagcg gcacctgcat gcgtttctgt
    43081 tcctcgaggc cgcgggcgaa gtggcgctgc gacaggtgcg ccagttcgtg ggccagcacc
    43141 gaggcgtact cgccctcggt ctgcgcgtgc aggaacaggc cgccattgac gccgatcact
    43201 ccgccgggcg cggcgaacgc gttgagctgc gggctgtcga ggaggacgaa gaccaggcgc
    43261 cggtcctgca gctggctggt ttccgccagg cgatagacgc tgttctcgat gaagtcctcg
    43321 agcagcgggt cggacaggct cttcacctgg ccgcgcagca cgttgagcca ggcgcggccg
    43381 agctggtgct cctggtcggg ggagacgatc gaggagctgg catcgccgag cgacggcagg
    43441 tcgctggcga gagccggggt cgccagcagg caggccaacg tcagcagggt ggggcgcaga
    43501 atcttcatcg ctcgaactcg cggatgcaaa ggcctactct agccgcagcg gcggctggct
    43561 ggccagtcgt gcgccgcctt gggtatgctg tgcggcattt gcggagagcc aacatgaccg
    43621 acactgccgg gccggcaccg gcccacgatg ccgaactgga tgccagcggg ctgaactgcc
    43681 cgttgccgct gctcaaggcc aagctggagc tcaaccgcct gcccagtggc gcggtactca
    43741 aggtgatcgc caccgatgcc ggttcgcagc gcgacttccg cgccttcgcc cggctggccg
    43801 ggcacgcgtt gctgcgcgag gaggaggagg ccggctgctt ccgttactgg ctgcgcaagg
    43861 cctgaggagg cagcggtcga cggctgcggc cgctcagccg gcgttgagcg cgcgagcggc
    43921 ttgcagtacg gcatccacat ggcccggcac cttcacgccg cgccattcct ggcgtagcac
    43981 gccgtccttg tcgatgagga aggtgctgcg ctcgatgccc aggtattcct tgccgtacag
    44041 tttcttcagc ttgatcacgt cgaacagctg gcacagttgc tcgtcggtat cggcaatcag
    44101 ctcgaagggg aactcctgct tgcacttgaa gttctcgtgg gacttcaggc tgtcgcgcga
    44161 gacgccgaaa atcagcgtgt tggcggcagc aaactcggca tgcaggtcgc ggaaattctg
    44221 gccctcggtg gtgcagcccg gggtgctgtc cttcggatag aagtacagca ccagctgccg
    44281 gcccttgagg gcgcccagcg cgacctgctg gccgctggtg gcctggacct ggaagtcggc
    44341 gacgggcgaa tcgatggcta ccggcatgat gaactcctgg tcggtggggc ttacgggttc
    44401 tgcgggcgcc acggctcgat cagagcgtcg aggttgaggg cgtcggcaaa gtcgaggaac
    44461 tgatcgcgca gccagctgat ctgggtgccc ggcggcaggg tcacggtgat ggtggcgttg
    44521 agcagcaggg tgccggtctg cggtgcctgg taggtgtcgc aggtgaggtt ctccagctcg
    44581 acgtggtgat cggtgaagaa ctggcacagc tcgccgatga tgtccgggcg ataggcggcg
    44641 ctgatataga ccacgtaggg cagggcattg gcgcgctcga cggcagcagc gctgcgggtc
    44701 acgttgatgc tgaacttgtg gcgcttggcc aggggcgcca ggctggcttc caggcgggcc
    44761 agcgcgtccc agctgccagc gacctgcagc accagggcgc tgtactcgcc atgccgggtc
    44821 aggcggctgc tgaccacgtt gcagcggctt tcctggctgg cgcgcgagag aacgctggtg
    44881 agctccatgg gcgaggctcc cagggcgctg atcacgagaa actgttcacg gggttggggg
    44941 gtcgacatgc agaattcctg gtgcaataga gatggcggca gcgcacgggc cggcagagcc
    45001 gggaagggta gcgaaaagcg ccgacaaggg gaatgcccgg tgacccgccg ccagcttgtg
    45061 caaggctggt ggcatcagta ccattacccc tttatttata tagcggcagg agtggttgca
    45121 tgattacggg cagcatggtg gcactggtca cgcccatgga tgcagagggc cgtctggatt
    45181 gggacagcct gacccaactg gtcgattttc acctccagga aggtaccaac gcgattgttg
    45241 cggtgggcac cacgggtgag tccgccacgc tggacgtcga cgagcacgtc gcggtaatcc
    45301 gtcatgtggt caaacaggtc gccgggcgca tcccggtgat cgccggtacc ggtgccaact
    45361 ccacccgcga agccatcgag ctgaccgccg ccgccaagca ggccggcgcc gatgcctgcc
    45421 tgctggtcac cccgtactac aacaagccga cccaggaagg cctgtacctg caccacaagg
    45481 ccatcgccga ggccgtggcc attccgcaga ttctctacaa cgtgccgggg cgtaccgcct
    45541 gcgacatgct gcccgacacc atcgcccgtc tgtccacggt gccgaacatc atcggcgtca
    45601 aggaagccac cggcgatctg gcgcgcggcc aggaagtgct ggacaaggtc gccccgggct
    45661 tcctggtcta ctccggtgac gacgccactg ccgtcgagct gatgctgatg ggcgccaagg
    45721 gcaacatctc ggtgaccgcc aacgtggccc cgcgtgacat gagcgagctg tgtgccctgg
    45781 ccatgcgtgg tgaggccgcg cccgcgcgcg ccatcaatga gcgcctgatg ccgctgcaca
    45841 aggcgctgtt ctgcgaggcc aacccgatcc cggtgaagtt cgccctgcac gagatgggac
    45901 tgattcccca aggcatccgc ctgcccctga cctggctcag ccagggctgc caggagccgc
    45961 tgcgtcaggc catgcgccag gccggtgtgc tggcctgatc gacggagaac atgcatgaag
    46021 caactggccg gactttccgc gcttgccctg atcattggaa ccagcagcgg atgcggctgg
    46081 atctggggcg acaacggcta cttccgcgac cgcggcagcg actatcttgc tgcccgcgaa
    46141 accgccccga tgcagctgcc gccggatgtc caggcgcgtc gtatcgagcc cctgctgccg
    46201 gtgccggcca gcgtggcgac ccgcagtgcc gaaggtgaat tcgaggtgcc gcgaccgcag
    46261 ggtctgcagg cgactgctca gcgcagcgac ttcagcctgc agcgcagcgg cgagcgtcgc
    46321 tgggtggtag cccagcgcag cccggccgag gtctggccgc tggcgcgcca gttcttcgag
    46381 gaaaacggcc tgagcatcag cgaggaacgt ccggagaccg gcgaactggt cagtgcctgg
    46441 cagaccgtgc cgaccgcgct tgcccgtcac ctcgacaagg cggatgccga agtcagcgtg
    46501 cgcctgcgcg tcgagcccgg cgtgcagcgc aacaccagcg aaatcttcct gctcagcaac
    46561 gtccgtcctg ccggcagctc ggccgagccg gcatggccgg cgcgctcggt ccagccggcg
    46621 ctggacggcg cgttgatcga gcgcctgcag agcagcctga gcagctccgt gggccagggt
    46681 ggcagcgttt ccctgctggc cgatcgcagc ttcgacgcgc ccaagcgcgt ctccctgggc
    46741 tccgatggca gtggcaaccc gcagctcagc ctggctaccg atttcgaccg cgcctggtcg
    46801 gcggtgggcc gtgccctgca ggatggcgat gtgcgcgtcg atgacatgaa ccgcagtctc
    46861 ggcatctact acatcaacct cgccgaaagc gcggacaagc cgggcgagga gccgggcttc
    46921 ttcgccggcc tgctgggcgg caaggcggat gccgaggacg tcgagtcccg cgccgagcgt
    46981 tatcatgtcc ggctgaccac cgtcggcgac agcgtgcagg tgtcggtcga gaaagacctc
    47041 gagaccgtcg cgccggccga tgtggcgcgc cgcgtgctgg agctgatcca ggaacacctc
    47101 ggctgagtcg ggcctgcagc cccagccgct accagcgata ggcgaaaccc ccgggtttcg
    47161 cctgttttgt ttgccagaaa cggaaatgcc gctatgacca cacccaccac cctgagcctg
    47221 aagaaaatct actcgggcaa ggttcgcgac ctctacgaaa tcgatgacaa gcgcatgctg
    47281 atggtggcca ccgatcgtct gtcggccttc gacgtcatcc tcgccgagcc gatcccggag
    47341 aagggcaaga tcctcaccgc catctccaac ttctggttcg acaagctcaa ggacctggtg
    47401 cccaaccact tcaccggcga caaggtcgag gacgtggtgc cggctgcgga gctgccgctg
    47461 gtggagggac gtgcggtggt ggccaagcgc ctcaagccgg tcgccgtgga agccatcgtg
    47521 cgcggttata tcgtcggctc cggctggaag gagtatcaga agagcggcac cgtctgcggc
    47581 atcgcgctgc cggccggcct gaaggaagcg gccaagctgc cgcagccgat cttcaccccc
    47641 tcgaccaagg ccgccgtcgg cgaccatgac gagaacatct ccttcgagca gtgcgaagcc
    47701 atcatcggca aggagctggc cgccaaggtg cgcgacaccg ccatcgccct gtacagcgcc
    47761 gcggtcgagt atgcggccac ccgcggcatc atcatcgccg ataccaagtt cgagttcggc
    47821 ctggacgagg acggcaccct gaccctgatg gacgaggtgc tgaccccgga ctccagccgc
    47881 ttctggccgg ccgacagcta cgtcgaaggg cagaacccgc cgagcttcga caagcagttc
    47941 gtgcgcgact ggctggaatc caccggctgg aacaaggagc cgccggcccc ggccgtgccg
    48001 gccgaggtgg cgcagaagac cgcggacaag taccgcgagg cgctgacccg gctgacccgc
    48061 tgaatagcgt cacggcgcgc aagcaactga tcctgcgcga aaaaatcctc acaaggggtt
    48121 ttgccaaacg gatctgagct ggtatgatgc gcgccgactt ggggagatgc cggagtggtc
    48181 gaacgggacg gattcgaaat ccgttgtgtc cgcgagggca cctagggttc gaatccctat
    48241 ctccccgcca tatatagaaa agccctgcaa atcaatggtt tgcggggctt tttcttttct
    48301 gattctaccc agtctttcta cccaagctta ctacccagtc cgtccgtctg ggagccatgt
    48361 tctcgcgtac ctgctgctgg cgttgggtgg ctgtagtatt ctgcaacgcc aacacatcac
    48421 gaccgaaggc agggatgcga agatatgaat gagaagcttt tctttctagc gagcaagaag
    48481 cggagcacca accacatcct gcacctgatt ctcactttgc ttacatttgg tgtgtgggtg
    48541 attgtctggg tgatcgttgc aagcaataac agtagctaca acagccgcat tgatgccgag
    48601 atggggcgca tcatcagcta caaggagaaa ggcttgagcg acgtacaggc atatcagcag
    48661 attagagcgg acaagacaaa ggaatctcag gacagagcga agactgtgat ggtggttttc
    48721 ggggtcatag tccttttcgt gatactcggg tcgctcggtg taaccccata gcgagggtcg
    48781 catggctcaa cgggggggat aaaccagaac cccggcctac cttgcgaata taaaatattg
    48841 gcaagagagg ctttataggg gggataggtc agaaggatag gctaagtttt tgcatgacgc
    48901 ggctgacctg cacaggggac cactcgccac cgcgagcagt cttcacccct ttggcattaa
    48961 gacaatctgc tacacccttc aacgtgcgag tgccacgcaa tacacacagc tctacagtgt
    49021 ccctaagact ctcagcaaag acttcagccc tttcttgggt ggcaatacca cccttcatct
    49081 gattggcctt tgtgcggccc ttggcaagcg atacagcacg atttgcaacc ttgccttgag
    49141 ctaccctgtc tccagcctcg gcacgcttag agaggctctg caaggcttcc ttggtgcgtt
    49201 ggctgataaa ttccctttcc tgctgggcga gcattgcgta caagtgcagc tccagggtct
    49261 tggctgctgg cattgtcgcc accttaaagt ctgaacgctt catcaggccg gcgatgtgtt
    49321 caacgtctcg gctaaggcgg tcaagcttcg ccaccaccac aggcaggcca tgagcaaacg
    49381 ccttggcagc ttctggacgc tccaaagggt gaacagagcc ggacacgttc acatcagtgt
    49441 attcagcgac caccacccaa ccgtgttgag cagcagcagt ggagacgtat tcccgctgag
    49501 cctccagacc taacccgctc tcgccttgac gcttggtgga tacccggtag taagcgacga
    49561 cttccattct cgatcccctt taacaatgct tgggatgatt atacgaatgc taggacgttc
    49621 gttcaagcta tgttatgccg gctttcagtg gctttccctg acacgccagt ggcagactga
    49681 gcaaaggttg aaaataaact aaggaagggt atccatgagc aacgccacgc ctgctgtgat
    49741 ccttggggga gcgatcatgg cagcagccgc catctacgtc gccccgtctg tgatagacga
    49801 ctaccagaat cagaacatca tcatcactac caacggggct gtgaggcttg gaaaggtcta
    49861 caacgaagcc ttgaaggtgc ggatggagat gaaaaacacc gaaacggggg aagtgttcat
    49921 ctccgtagac gaagatgttg atggatttta taaacaagca aggtcggagc ttgagaagct
    49981 gatccacgcc acgaatgagg gcgcgaagtc tgacaaggaa aagatttccg ttgaggctgc
    50041 gggtctcaag gtacatatga ccatcactct tgatacctat ttcctctatc gtagtgaata
    50101 ccagccatcc ttcagtctga ccgtcgatca gcacgaaatc tcggcggggg ttaattcagg
    50161 tatcaacaag gttttgatgc agacccagga gtacttgaat gaagtgaaaa ttgagaatct
    50221 tgaaaagaat agattcctaa agctttaacg gcctgcctcc ttgctgagtg tgttggtggc
    50281 tggttaagca tatctgccat gtaggctgca tagaatgggc ctgttgagct tctggggtat
    50341 ggaaagggag ggcaggcagc tcacctgcct cctatttgat tgtctgttta tcttgctttt
    50401 ttattcttga acatgttctt gatgctgtct ttgtggtcca gctttttggc aggtttattt
    50461 gttgtgtcaa tttgttggct gggcttgtgg gtggcttgag ccttatttgc tttgtattcg
    50521 tccagcagag cttgaatacg tgcctctttt tcagcagcaa gcttggcact ttctttttcc
    50581 tgtagaagct cttggtgtcg ctcagggtgg aagcgctcaa gaattagctc gtgggttctt
    50641 tgtgctgata gtgatacgaa ttgttggtta atcatttttt attattccgt agatttgtga
    50701 gggggcgagc taagtcgccc cttgttgttt tggttacttg ggcaggtagg agtcgaaaag
    50761 gtctgctgct tctttctcca gttctcgatg tagcttttca tctgccttgc gcttcttctc
    50821 atcctcttgc cgctggatag cggccttacg ctgaatgatt agattttgtt tggcttcatc
    50881 cacctgtgcg gcaatctcgc tcctgtaatc cgcttccact tgctgtctga cggtattcag
    50941 ctccgcttcc acctcctcag gtttcttaaa catcgggatg acgaagggat ggctttggtg
    51001 gttcgtttcc cgtgctcgtt gatacccctc ttgtagcttt tgttcgagaa ggtcaagtgc
    51061 ttggaaaaga atctctgcct tgatggtgaa aatagtgatg tttggaagat taaggtaatc
    51121 cgtgacagct tgatcctggc gggctttaag ctctttaggg tcaaaggtgg ctggcatctt
    51181 tacggtaatt tcttggccgt caataatggt cttgctcatt ttttgttttc cttcttttgt
    51241 tttgattagg caatgtctgc ccgtttactg ctcttgttta gaaagcaatt ctttggtgtt
    51301 tgtctttgag gctggcgctt tggaaaggcg ggaaaagcat cttagacttc gctggcgcta
    51361 cgtcttaccg acatcgctac gcaatgtctc gtcaataatc gctcgctatg cttcgctctt
    51421 attgactaac cttaaactat atatataaaa gatgctgttc aggcgccata gtcgccataa
    51481 tgctctaaac cgcgcatgta ctgggcatta cagctatggc gggtattatg g
//
LOCUS       DATCUN010000262         2859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_49731, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000262 DATCUN010000000
VERSION     DATCUN010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49731"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VIO83_10265"
     CDS             complement(<1..401)
                     /locus_tag="VIO83_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014338432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8331697.1"
                     /translation="MRPLLPCLFTALGLLAAGPASAELEVRIGYLGYTPDRGPVLSNV
                     IPEPTDAGRRGAELAIEDSNSTGRFLKHSYRLESAEAASGEALLEQARALHDGGLRLF
                     VVNAPADTLRQLAGALPDSLLLNAGSADDSLR"
     gene            562..894
                     /locus_tag="VIO83_10270"
     CDS             562..894
                     /locus_tag="VIO83_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331698.1"
                     /translation="MSLLRLLSLLVLLILVLLGLDPTQSSAADLAARRAAAQARLLTL
                     QTGACQAAPVLPAGGAANGARRPACTGFTGKALPMDSYQGTKARAPKQNTVRAGGLPK
                     MPTTRSTG"
     gene            931..1368
                     /gene="pedF"
                     /locus_tag="VIO83_10275"
     CDS             931..1368
                     /gene="pedF"
                     /locus_tag="VIO83_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-550 PedF"
                     /protein_id="HEY8331699.1"
                     /translation="MNNKKLLHALLASLALGAASHAMAHGDVTPQAVNTADLEALGSE
                     WRDENPYREPYANHKKAVEIGASAYTQNCARCHGLEAVSGGIAPDLRMLDEGIEGDEW
                     YKERVINGAVRDGRVYMPKMADYLSQESLWAIRSYLESVAVKE"
     gene            1375..2280
                     /locus_tag="VIO83_10280"
     CDS             1375..2280
                     /locus_tag="VIO83_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8331700.1"
                     /translation="MRLLILVLCSLLTLAQVAQAQVRAFDDIVASGVLKAAVYENFPP
                     YSFQQDGQPRGVDVELAQQLAASQGLKLELLWIAAGEKLDDDLRNFVWKGHYLRPEVL
                     ADVMLRVPYDRNYAFKRNELGELVNERVVMFAPYQRERWQVAHDARRLKEVPSVAVFG
                     YHPIGVEVESVPSFYLSSVFGGRMSGNTRHYPSPPQAFAAMRAGKVDAVMAMRGEIDW
                     LLHEAADPQLKAAENAYPEMGRQQWDIGMAVHESNRQLAYALESGLEELIGSGELAKI
                     YARYGLRYEVPELYEGEVQAASTGE"
     gene            2434..>2859
                     /locus_tag="VIO83_10285"
     CDS             2434..>2859
                     /locus_tag="VIO83_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017243924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HEY8331701.1"
                     /translation="MGLALGWLLLSSPAWAGGADPLQSAMWGFHQTRFLGEAPYEFDA
                     RVVLTAPPFAEDSRQVPISIDATALGSEVVRMIAWAELNPIPHIFTFFPSGPVAPRIA
                     LNIRVEQSTPIRAAVLTRDGTWHVGSTRIDAAGGGCTAPS"
BASE COUNT          460 a          953 c         1024 g          422 t
ORIGIN      
        1 cgcaggctgt cgtcggcgct gccggcgttg agcagcaggc tgtccggcag cgcgccggcc
       61 agctggcgca gggtgtcggc gggggcgttg accacgaaca ggcgcaggcc gccgtcgtgc
      121 agcgcgcggg cctgttccag cagcgcttcg ccgctggcgg cctcggcgct ttccaggcgg
      181 tagctgtgct tgaggaagcg cccggtgctg ttgctgtcct cgatggccag ctcggcgccg
      241 cgccggccgg cgtcggtcgg ctcggggatc acgttggaca gcaccgggcc gcggtcgggg
      301 gtgtagccga ggtagccgat gcgcacctcc agctcggcac tggccggacc ggcggcgagc
      361 aggccgaggg cggtgaacag gcagggcagc agtgggcgca tggcaacctc cggtggggat
      421 gctgccagct taggaagggg gcgggggcgg gcaaatatgc agaaagtact ggtggggcag
      481 taccaaggta ccggtccggt gccggaaggc agctcttagc atggggctta caacgataac
      541 gacaggaaag gcccctcgcc catgtccctg ctcagattac tgtccctgct ggtgctgctg
      601 atcctcgtcc tgctcggtct cgaccccacc cagagcagcg ctgccgacct tgccgcccgt
      661 cgcgccgcgg cgcaggcccg gctgctgacg ctgcagaccg gcgcctgtca ggccgcgccc
      721 gtactgccgg ccggtggtgc cgcgaacggc gcgcggcgcc cggcgtgcac cgggttcacg
      781 ggcaaggcgc tccccatgga ctcctaccaa ggaacgaagg cgcgggcacc aaagcagaat
      841 acagtccgcg ccgggggcct ccctaagatg ccgacaacgc gctccacagg ctgagcgccg
      901 ccttgcaccg catcgaaacg aggtaccgcc atgaacaaca agaaactgct gcacgccctg
      961 ctggcttccc tggcgctcgg cgccgcttcc catgccatgg ctcatggcga cgtgaccccg
     1021 caggcggtga ataccgccga cctggaggcg ctgggtagcg agtggcgcga cgagaacccc
     1081 taccgcgagc cctacgccaa ccacaagaag gcggtggaaa tcggtgcttc ggcctacacc
     1141 cagaactgcg cccgctgcca cggtctggaa gccgtctccg gcggcatcgc cccggacctg
     1201 cgcatgctgg atgaaggcat cgagggcgac gagtggtaca aggagcgggt gatcaacggc
     1261 gcggtgcgcg atggtcgcgt ctacatgccg aagatggccg actatctcag ccaggaatcg
     1321 ctgtgggcca tccgcagcta cctggaaagc gttgccgtca aggagtaagc cgccatgcgc
     1381 ctgctgatcc tggtgttgtg cagtctgctg acgcttgccc aggtcgcgca ggcgcaggtt
     1441 cgcgcgttcg atgacatcgt cgcctcgggc gtgctcaagg cggcggtgta cgagaacttc
     1501 ccgccctaca gcttccagca ggacggccag ccgcgcggcg tggatgtcga gctggcgcag
     1561 cagctggccg ccagccaggg cctcaagctg gagctgctgt ggatcgcggc gggcgagaag
     1621 ctcgacgacg acctgcgcaa cttcgtctgg aagggccact acctgcgtcc cgaggtgctt
     1681 gccgacgtga tgctgcgggt gccctacgac cgcaactacg ccttcaagcg caacgagctg
     1741 ggcgagctgg tcaacgagcg ggtggtgatg ttcgccccct accagcgcga gcgctggcag
     1801 gtcgcccatg acgcccgccg gctcaaggaa gtgcccagcg tcgcggtgtt cggctaccac
     1861 ccgatcggcg tcgaggtgga gagcgtgccg tcgttctacc tgagctcggt attcggcgga
     1921 cggatgagcg gcaatacccg ccactacccc tcgccgccgc aggcgtttgc ggcgatgcgc
     1981 gcgggcaagg tggacgcggt gatggccatg cgcggcgaga tcgactggct gctgcacgag
     2041 gccgccgatc cgcagctcaa ggcggcggaa aacgcctatc cggaaatggg tcgccagcag
     2101 tgggacatcg gcatggccgt gcacgagagc aaccgccaac tggcctacgc gctggaaagc
     2161 gggctggaag agctgatcgg ttccggcgag ctggcgaaga tctacgcccg ctacggcctg
     2221 cgctacgagg tgccggagct gtacgagggc gaggtgcagg cggcatccac gggcgaatga
     2281 cggctgatcg ctcccacgct cccgcgtggg agcgtctccg cgtcccgagt tgccgggcgc
     2341 aggagcgccc atcgggatcg gtacccacgc cggagcgtgg gcaccatcag cagacacggg
     2401 taacgcagga ggcaggcaat gaccatccgt agcatgggcc tggcgctggg ctggctgttg
     2461 ttgagcagcc cggcctgggc cggcggggcc gatccgttgc agtcggcgat gtggggcttc
     2521 caccagacgc gcttcctcgg cgaggcgccc tacgagttcg acgcgcgggt ggtgctgacg
     2581 gcgccgccgt tcgccgagga ctcgcgccag gtgccgatca gcatcgacgc cacggcgctg
     2641 ggcagcgagg tggtgcgcat gatcgcctgg gctgagctca acccgatccc gcacatcttc
     2701 accttcttcc ccagcggacc ggtggcgccg cgcatcgcgc tgaacatccg tgtcgagcag
     2761 tccacgccga tccgcgccgc ggtgctgacc cgcgacggca cctggcatgt cggctcgacg
     2821 cgcatcgacg ccgccggcgg cggctgcacc gcgccgagt
//
LOCUS       DATCUN010000263        10339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_199728, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000263 DATCUN010000000
VERSION     DATCUN010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10339
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199728"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VIO83_10290"
     CDS             <1..519
                     /locus_tag="VIO83_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEY8331702.1"
                     /translation="AREPLVRLAQAIAVACRAEPGLNAWFDGKALAMRRHEQLDLGIA
                     VDTPDGLFVPVLRNVGQRDADDLREGMRRLRADVKARSIPPQEMMGATVTLTNFGTLF
                     GRYASPVVVPPQVAIIGAGVIRDQPVAVDGRVEVHPILPLSLTFDHRAVTGGEAGRFF
                     KALVEALQQPEG"
     gene            656..1222
                     /gene="mobA"
                     /locus_tag="VIO83_10295"
                     /pseudo
     CDS             656..1222
                     /gene="mobA"
                     /locus_tag="VIO83_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
     gene            1349..1825
                     /gene="moaC"
                     /locus_tag="VIO83_10300"
     CDS             1349..1825
                     /gene="moaC"
                     /locus_tag="VIO83_10300"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY8331703.1"
                     /translation="MLTHLDAQGRANMVDVTDKAATAREASAEALVRMQPQTLKLIVS
                     GGHPKGDVFAVARVAGIMAAKKTHELIPLCHPLLLTGIRVELEPRGEDAVHIVARCKL
                     AGQTGVEMEALTAASVAALTIYDMCKAVDRGMTIERVRLLEKSGGKSGDYRAEEVR"
     gene            1822..2070
                     /locus_tag="VIO83_10305"
     CDS             1822..2070
                     /locus_tag="VIO83_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY8331704.1"
                     /translation="MIEVRYFARYREALGSDGERLEWSATLDSLEALRRQLLERGGPW
                     LVLAEKSLLCARNQELCSLADPLADGDEVAFFPPVTGG"
     gene            2072..2521
                     /gene="moaE"
                     /locus_tag="VIO83_10310"
     CDS             2072..2521
                     /gene="moaE"
                     /locus_tag="VIO83_10310"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HEY8331705.1"
                     /translation="MVIRVQVAAFQPGVELEVLHAANAGIGAVVGFVGYVRDYNDGNE
                     VAAMTLEHYPGMTEKALAGIVEQARARWPLLAVEIVHRVGHLQPGEPIVFVGTASSHR
                     QAAFDACNFIMDYLKTRAPFWKREDTPEGARWVDGRDSDQAAAQRWT"
     gene            2744..3739
                     /gene="moaA"
                     /locus_tag="VIO83_10315"
     CDS             2744..3739
                     /gene="moaA"
                     /locus_tag="VIO83_10315"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY8331706.1"
                     /translation="MNDAMLLDGHGRKIDYLRLSVTDRCDFRCVYCMAEEMTFLPRQQ
                     VLGFEEIVRIARLFVELGVKKIRLTGGEPLVRRGIVDLCRQIASLPGLRELVMTSNGS
                     QLGKLARPLAEAGVKRLNISLDSLDAERFHAITRTGDLPQVLAGIEAARDAGFARIKL
                     NSVIMKGRNDDEVAALVDFAVARGLDISFIEEMPLGQVGRDRGEAFCSSDEVRARIAE
                     RYNLFDSPEQSGGPARYVRLEGHPDTRVGFISPHSHNFCSSCNRLRLTAEGRLLLCLG
                     HEHSLDLRGLVRRHPTEDRPLVEAIGAALQRKPLRHEFAVSGEVQVVRFMNATGG"
     gene            complement(3755..4015)
                     /locus_tag="VIO83_10320"
     CDS             complement(3755..4015)
                     /locus_tag="VIO83_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebG family protein"
                     /protein_id="HEY8331707.1"
                     /translation="MAVEVVYRSSRDLERLFMDKAEADRHDRMLELAEKLAEVLRQAQ
                     PALDERQAEELGIYMARHRELFAKAFRNQTDALDELLTPTGG"
     gene            4208..4528
                     /locus_tag="VIO83_10325"
     CDS             4208..4528
                     /locus_tag="VIO83_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8331708.1"
                     /translation="MTEAQADLDMQLMRSAASEAAAVLRTLGNPDRLLLLCQLSQGER
                     SVGELEELLGLRQPSLSQQLAVLRNEGLVQTRRDGKRMFYSIAEPKLLVLLGTLYQLY
                     CPQA"
     gene            4668..5927
                     /locus_tag="VIO83_10330"
     CDS             4668..5927
                     /locus_tag="VIO83_10330"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEY8331709.1"
                     /translation="MSFNKPLFAALLAGSAAISPLAFATNGYFAHGYGIKSLGMAGVG
                     YALPQDALAAATNPAGTALVGDRLDLGVTYFKPDRSVEIHGAGAPGANGQYDGNDTDA
                     FLIPEFGYTRQLDERLAFGLAVYGNGGMNTDYQRNPFAAYGSTGDAGVDLTQVFVTPS
                     LAWRLNERHSFGIGLNYAYQRFEARGIDGFARMSESPEHVSNNGHDHSDGWGLRLGWN
                     GQLTDTLTVGVAWSSKIKTGKFDRYEGLFADHGSFDIPENYGIGLAWRATERLTLAAD
                     VQQIDYSDVKSISNPVSVLFSGQALGSEDGPNFGWEDIRVYKLGASYAVSPQLTLRGG
                     YSWADQALDKGETFFNTLAPGVVREHLTLGATWAPEADHEISVFYAHAFSEELKGSDS
                     IPQQFGGGEVDLQMQQDSFGIAYAWKL"
     gene            6043..6513
                     /locus_tag="VIO83_10335"
     CDS             6043..6513
                     /locus_tag="VIO83_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331710.1"
                     /translation="MQDNASPVAGQVGYVAIEQPFGLQPPKVHCPICGQQQFVEQDDE
                     YLETPCEHLVFVFGGEEDEFVYASDDFAARFARFDAELDARFPDDEARASAEYEEASS
                     FYRDLFPLRLAELGYGASLLALRVTYGGMAEGSPVWFSDVYGFDYALIREDDAE"
     gene            6546..6983
                     /locus_tag="VIO83_10340"
     CDS             6546..6983
                     /locus_tag="VIO83_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016780719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEY8331711.1"
                     /translation="MLDLSAYLGLFFAAFGAASLLPLQSEALLVGLLLAERQPVLTLL
                     AVATAGNVLGSLLNWLLGLYLERFREKRWFPVRERHLQRARHWYQRFGRWSLLFSWLP
                     VVGDPLTLVAGVMRESFWVVLACVTLAKGVRYAALTALTLGLA"
     gene            7125..7364
                     /locus_tag="VIO83_10345"
     CDS             7125..7364
                     /locus_tag="VIO83_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017136600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2789 domain-containing protein"
                     /protein_id="HEY8331712.1"
                     /translation="MEQPIHAFHHLFEQLGLPNDNQAISQFLERHAPLPEDVELADAS
                     FWTPAQAAFLREEILEDADWAEVIDNLNAALRQRQ"
     gene            complement(7509..9065)
                     /gene="ahpF"
                     /locus_tag="VIO83_10350"
     CDS             complement(7509..9065)
                     /gene="ahpF"
                     /locus_tag="VIO83_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEY8331713.1"
                     /translation="MLDANLKTQLKAYLEKVTQPFEIVASLDDGAKSQELLGLLTDIA
                     SLSDKIALRTDGDDSRKPSFALNRINGDISLRFAGIPMGHEFTSLVLALLQVGGHPSK
                     TSADVIEQIKNLEGEFNFETYFSLSCQNCPDVVQALNLMAVLNPNIRHVAIDGALFQD
                     EVEQRQIMSVPSIYLNGELFGQGRMGEEEILAKLDTGASARDAEKLNAKDAFDVLVVG
                     GGPAGATAAIYAARKGIRTGVAAERFGGQVLDTMAIENFISVPETEGPKLARALEEHV
                     RQYDVDIMNLQRAAKLVPAASEGGLHEVQFDNGATLKAKTVIVATGARWREMNVPGEQ
                     EYRGRGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLLEFGEQLRAD
                     AVLQNKLRSLPNVTILTMAQTTEVVGDGQKVTGLVYKDRGSEEVHTVELEGIFVQIGL
                     LPNSDWLKGTVELTRFGEVITDAKGQTNIPGVFGAGDVTTVPYKQIVIAVGEGAKAAL
                     SAFDHLIRQV"
     gene            complement(9184..9747)
                     /gene="ahpC"
                     /locus_tag="VIO83_10355"
     CDS             complement(9184..9747)
                     /gene="ahpC"
                     /locus_tag="VIO83_10355"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HEY8331714.1"
                     /translation="MSLINTQVQPFKVNAFHNGKFIEVTEQTLKGKWSVMIFMPAAFT
                     FNCPTEIEDAAENYAEFQKAGAEVYIVTTDTHFSHKVWHETSPAVGKAQFPLIGDPTH
                     ALTNAFGVHIPEEGLALRGTFIINPEGVIKTLEIHSNEIARDVSETLRKLKAAQFTAA
                     HPGQVCPAKWKEGAETLTPSLDLVGKI"
     gene            9981..10338
                     /gene="ssrA"
                     /locus_tag="VIO83_10360"
     tmRNA           9981..10338
                     /gene="ssrA"
                     /locus_tag="VIO83_10360"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1625 a         3340 c         3492 g         1882 t
ORIGIN      
        1 gcgcgcgaac cgctggtgcg cctggcccag gccatcgccg tggcctgccg cgccgagccg
       61 gggctcaacg cctggttcga cggcaaggcg ctggccatgc gccgccacga gcagctcgac
      121 ctgggcatcg ccgtggacac cccggacggc ctgttcgtgc cggtgctgcg caatgtcggc
      181 cagcgtgatg ccgatgacct gcgcgaaggc atgcgccgcc tgcgcgcgga cgtcaaggcg
      241 cgctcgattc cgccgcagga aatgatgggc gcgaccgtga ccctgaccaa tttcggcacc
      301 ctgttcggcc gctatgccag ccccgttgtg gtgccgccac aggtggcgat catcggtgcc
      361 ggggtgatcc gcgaccagcc ggtggcggtg gacggccggg tcgaggtgca cccgatcctg
      421 ccgctgtcgc tgaccttcga tcatcgcgcg gtcaccggcg gcgaggcggg gcgcttcttc
      481 aaggccttgg tcgaagcgct gcagcagccg gagggctgag cctgcgcagg gcgaaggcat
      541 ccgcttgcgc ccgccgccgt ccggttcgat gactgcggta gggtgaacgc gtcggctggc
      601 ggcgcgcgtg tggcggtgta ccatcacgcc aagaccgacc tcttggagta tcgccatgct
      661 tgttgctgcc gctgacctgc ccccctgttc cgtgctgctg ctcgccggcg ggcgagggcg
      721 gcgcatgggc ggggtggaca aggggctggt ggagtttgcc ggccgcccgt tgatcgccac
      781 gctgagcgaa cgggtgcagc cgctgaccga cgacctgatc atctcctgca accgcaacgc
      841 cgagagctat gcgctgtggg ccgaccgcct ggtgagcgac gacggcgacg attttcccgg
      901 cccgctggcc ggtatccgcg ccggcctcgc cgcgatgcgc cacgactggc tgctggtgct
      961 gccctgcgat gcgccgcata tcgacgccga cctgctgttt tccatgcgcc accaggccgt
     1021 gtgcgagccg cagcgtccgt tgctgctgcg ccgcggcggg cagttggagc cgctgttctg
     1081 cctgattccg cgctgcctgc agggcgagct ggagcgcgcc tggcaggccg gcgagcgcag
     1141 cccgctgagt ttcctgctgg cgtgcaacgc ccaggtactg ggctgtgcac aggacgatcc
     1201 gcggctggcc aatctgaata cgctcgaggc gctggtggcg gccgggccgc gcggcgcccc
     1261 gcaggacgag tccgacgaag agtcccgccc gcagctgccc gatgcggcag gcctggcgca
     1321 tgccgcgtcg gcctccggga caacccccgt gctgacccat cttgacgccc aggggcgggc
     1381 caacatggtc gatgtcaccg acaaggccgc caccgcccgc gaagccagtg ccgaggcgct
     1441 ggtacgcatg cagccgcaga ccctcaagct gatcgtctct ggcggccacc ccaagggcga
     1501 cgtcttcgct gtggcgcggg tggccgggat catggcggcg aagaagaccc acgagctgat
     1561 cccgctgtgc catccgttgc tgctcaccgg cattcgtgtc gaactggagc cgcgggggga
     1621 ggacgcggtg catatcgtcg cccgctgcaa gctggccggg cagaccgggg tggagatgga
     1681 ggcgctgacc gccgccagcg tcgccgcgct gacgatctac gacatgtgca aggcggtgga
     1741 ccgcggcatg accatcgaac gcgtgcgcct gctggaaaag agcggcggca agagcggcga
     1801 ctaccgtgcg gaggaggtcc gatgatcgag gtgcgctatt tcgcccgtta ccgcgaggcg
     1861 ctgggcagcg atggcgagcg cctggaatgg tcggccacgc tggacagcct cgaggccctg
     1921 cgccgccagc tgctggaacg tggcggcccc tggctggtac tggccgagaa gagcctgctg
     1981 tgcgcacgca accaggagtt gtgcagcctc gccgatccgc tggccgacgg tgacgaggtg
     2041 gccttcttcc caccggtgac cggaggctga catggtaatt cgggtccagg tcgcggcctt
     2101 ccagcccggc gtcgagctgg aggtgctgca cgccgccaac gccggcatcg gtgcggtggt
     2161 cggcttcgtt ggctatgtgc gcgactacaa cgacggcaat gaggtggcgg cgatgaccct
     2221 cgagcactat ccgggcatga ccgagaaggc tctggccggc atcgtcgagc aggcgcgcgc
     2281 gcgctggccg ctgctcgcgg tggagatcgt ccaccgcgtc ggtcacctgc agccgggcga
     2341 gccgatcgtc ttcgtcggca ctgccagcag ccatcgccag gccgccttcg acgcctgcaa
     2401 cttcatcatg gattacctga agacccgcgc gccgttctgg aagcgcgagg atacgcccga
     2461 gggcgcgcgc tgggtcgatg gccgcgacag cgaccaggcg gcggcgcagc gctggacgtg
     2521 agtgccgccg gcgccgtatg ctcggcaatg gtgcagtgac gaatgcgtgc acttcgccgc
     2581 gccccgcgcg ggtgcgcggc cgcctcggcc aaaaaacggg caacgtgctt aactatgggc
     2641 gatggtctgc ctgcgcggcg gctgtgccgg tgctggtgca gtccttgcaa tgtgcatggc
     2701 acaccgcccg actgctcggc gcggccacga ggagaaacgc cagatgaacg acgccatgct
     2761 gctcgacggc catggtcgca agatcgacta cctgcgcctg tcggtgacgg atcgctgcga
     2821 ctttcgctgt gtctactgca tggccgagga gatgaccttc ctcccgcgcc agcaggtgct
     2881 cggcttcgag gagatcgtgc gcatcgcccg cctgttcgtc gagctcggcg tgaagaagat
     2941 ccgcctgacc ggcggcgagc cgctggtacg tcgcggcatc gtcgacctgt gccggcagat
     3001 cgcgtcactg ccgggcttgc gcgagctggt gatgaccagc aacggctcgc aactgggcaa
     3061 gctcgcccgg ccgctggccg aggcgggggt caagcgtctg aacatcagtc tcgacagcct
     3121 ggatgccgag cgcttccacg ccatcacccg caccggcgat ctgccccagg tgctggccgg
     3181 catcgaggcg gcgcgcgacg ccggctttgc ccgcatcaag ctcaacagcg tgatcatgaa
     3241 gggtcgcaac gacgacgagg tggcggcgct ggtggatttt gccgtggccc gcggcctgga
     3301 tatcagcttc atcgaggaga tgccgctggg ccaggtgggt cgcgatcgcg gcgaggcgtt
     3361 ctgctccagc gacgaggtgc gcgcgcggat cgccgagcgc tacaacctgt tcgacagtcc
     3421 cgagcagagc ggcgggccgg cgcgctacgt gcgtctggag ggccatcccg acacccgcgt
     3481 cggcttcatc tcgcctcact cgcacaactt ctgctcaagc tgcaaccgcc tgcgcctgac
     3541 tgccgagggc cgtctgttgc tgtgcctggg tcacgagcat tcgctggacc tgcgtggcct
     3601 ggtgcgtcgt catccgaccg aggatcggcc gctggtcgag gccatcggcg ccgccctgca
     3661 acgcaagccg ctgcgccacg agttcgcggt gtccggcgag gtgcaggtgg tgcgcttcat
     3721 gaatgccacc ggcggctgag attgcgccgg ggcgtcaccc gccggtgggg gtgagcagct
     3781 cgtccagcgc gtccgtctgg ttgcggaagg ccttggcaaa cagctcgcgg tgtctcgcca
     3841 tgtagatgcc gagttcctcg gcctggcgct cgtcgagtgc cggttgcgct tgccgcaaca
     3901 cctcggccag cttctccgcc agttcgagca tccggtcgtg acgatctgct tcggctttat
     3961 ccatgaacaa acgctccaaa tcccggctgc tgcggtaaac cacttcgacg gccatgcgcc
     4021 acctcatctg cctgcataat gctggttgac tgttttttta tacagtaatg gtgggcatta
     4081 tagacagcca ctgcgcgcct gcataggaat gctggcaggg tgacaggcat ggccgtggca
     4141 gagtgttcgc gcgatactgt cgcgccattt atatccaccg gcccgcgaca gaaggaatcc
     4201 tcgcgccatg accgaagccc aagccgatct cgacatgcag ctgatgcgca gcgccgccag
     4261 cgaagcagcc gccgtgctgc gcaccctcgg caatcccgat cgcctgctgc tgctctgtca
     4321 gctgagccag ggcgagcgct cggtgggcga actggaagag ctgctcggtc tgcgtcagcc
     4381 gagtctgtcg cagcagctgg cggtgctgcg caacgagggg ctggtgcaga cgcggcgcga
     4441 cggcaagcgg atgttctatt cgattgccga gccgaagctg ctggtgctgc tcggcaccct
     4501 ctaccagctc tactgtccgc aggcctgacg gcggcggcag ttcggcctta tagaaccaaa
     4561 gtgaatcaat acataatcta atattataat tgcatattat gtattttgtt taagattacc
     4621 tccattcagg tttgcctcgg gcagccgact gttttggggg aatctgcatg tcgttcaaca
     4681 agccgctgtt tgccgccctg ctcgccggca gtgccgccat tagtccgctg gccttcgcca
     4741 ccaacggtta tttcgcccac ggttacggca tcaagtccct cggcatggcc ggtgtcggct
     4801 atgccctgcc gcaggatgcc ctggccgctg cgaccaatcc ggccggtacg gcgctggtgg
     4861 gcgaccgcct ggatctgggc gtgacctact tcaagcccga ccgcagcgtg gagatccatg
     4921 gcgccggcgc tccgggcgcc aacggccagt acgacggcaa cgataccgac gccttcctga
     4981 tccccgagtt cggctatacc cgtcagctcg acgagcgcct cgccttcggc ctggcggtgt
     5041 acggcaatgg cggcatgaac accgactacc agcgcaatcc gttcgcagcc tacggcagca
     5101 ccggtgatgc tggcgtcgac ctgacccagg tcttcgtcac tccgtcgctg gcctggcgcc
     5161 tcaacgagcg ccacagcttc ggcatcggcc tcaactacgc ctaccagcgc ttcgaggcgc
     5221 gcggcatcga cggttttgcc cggatgtccg agtcgccgga gcatgtcagc aacaatggcc
     5281 acgaccattc cgatggctgg ggcctgcgcc tgggctggaa cggccagctc accgatacgc
     5341 tgaccgtcgg tgtggcctgg tcgtcgaaga tcaagaccgg caagttcgac cgctacgaag
     5401 gcctgttcgc cgaccacggc agcttcgaca ttccggagaa ctacggcatc ggcctggcct
     5461 ggcgcgccac cgagcgcctg accctggccg cggacgtgca gcagatcgac tacagcgacg
     5521 tgaagtcgat ctccaacccg gtcagcgtgc tgttcagcgg tcaagcgctt ggctccgagg
     5581 acggccccaa cttcggctgg gaagacatcc gcgtgtacaa gctcggcgcc agctacgcgg
     5641 tcagtccgca gctgaccctg cgcggcggct acagctgggc agaccaggcg ctggacaagg
     5701 gcgagacctt cttcaacacc ctggcgccgg gcgtggtgcg cgagcacctg acccttggcg
     5761 ccacctgggc gcccgaggcc gaccacgaga tcagcgtgtt ctacgcccac gccttcagcg
     5821 aggagctcaa gggcagcgac tcgattccgc agcaatttgg cggcggcgag gtggatctgc
     5881 agatgcagca ggacagcttc ggtatcgcct acgcctggaa gctctgatcg ggcggcgatt
     5941 tctcgcccac agtcgagccc gaacacttaa acttgccgca cccacggggc gaccgatcag
     6001 gtcgccccgt ttcgttgcag ggtcgatttc agcgaggatt tcatgcagga caacgcttcc
     6061 ccggttgccg gtcaggtcgg ttacgtcgcc atcgaacagc cgttcggctt gcagccgccc
     6121 aaggtgcact gcccgatctg tggccagcag cagttcgtcg aacaggacga cgagtacctg
     6181 gaaaccccct gcgagcacct ggtcttcgtc ttcggcggcg aggaagacga gttcgtctac
     6241 gcttcggatg acttcgccgc gcgtttcgcc cgcttcgatg ccgagctgga tgcgcgcttc
     6301 cccgacgacg aggcgcgggc cagcgccgag tacgaggagg ccagcagctt ctaccgtgac
     6361 ctgtttcccc tgcgtctggc cgagctgggc tacggcgcca gcctgctggc cctgcgggtg
     6421 acctacggcg gcatggccga gggcagtccg gtgtggttca gcgatgtcta tggtttcgac
     6481 tacgcgctga tccgcgagga cgacgcagag taaggctttc ccctcgaggt gccgcagcac
     6541 tcgccatgct cgatctttcc gcctatctcg gtctgttctt cgccgccttc ggcgcggcca
     6601 gtctgctgcc gctgcagtcc gaggcgctgc tggtcggcct gctgctggcc gaacgccagc
     6661 cggtgctgac cctgctggcg gtggccaccg ccggcaacgt gctcggctcg ctgctcaact
     6721 ggctgctggg cctgtatctc gagcgcttcc gcgagaagcg ctggttcccg gtgcgcgagc
     6781 ggcacctgca gcgcgcccgg cactggtatc agcgctttgg ccgctggtcg ctgctgttca
     6841 gttggctgcc ggtggtcggt gatccgctga ccctggtggc cggggtgatg cgcgagtcgt
     6901 tctgggtcgt gctggcctgc gtcaccctgg ccaagggtgt ccggtatgcc gcgctgacgg
     6961 cgctgaccct gggactggcc tgaacgggct gacgctgcgc tatcggtacg agccgtaccg
     7021 cgccattggg cggccagcac cgcgctggcg ccggcaggtt cgcccgctct gggcaatact
     7081 ctgagctgtt gccaaccatg ctgcaggcat tgccggagga ttccatggaa cagccgatcc
     7141 acgcctttca tcacttgttc gagcaactgg gcttgcccaa cgacaatcaa gcgatcagcc
     7201 agtttctcga gcgtcatgcg ccgctgccgg aggatgtcga gctggccgac gccagcttct
     7261 ggactccggc gcaggccgcg ttcctgcgcg aggagatcct cgaggatgcc gactgggccg
     7321 aggtgatcga caatctgaac gcggcgctgc gccagcgcca atgaatggcc gggcgtgctc
     7381 gcttgcgcct gagcgtgccc cgcggccgtg agccgggtgg gtgggctgtc gctgccgcgc
     7441 ggggcggccc ttgcgccaac aaaaacgccg cgccctcccg gagggcgcgg cgcggatgag
     7501 gcaggcgatc agacctggcg gatcaggtgg tcgaaggcgc tcagcgcggc cttggcccct
     7561 tcgcccacgg cgatgacgat ctgcttgtag ggcacggtgg tcacatcgcc ggcgccgaac
     7621 acgcccggga tgttggtctg ccccttggcg tcggtgatga cctcgccgaa gcgggtcagc
     7681 tcgacggtgc ctttcagcca gtcgctgttg ggcagcagac cgatctgcac gaagatgcct
     7741 tccagctcga cggtgtggac ctcctcgctg ccgcggtcct tgtacaccag gccggtgacc
     7801 ttctgcccat cgcccacgac ctcggtggtc tgcgccatgg tcaggatggt gacgttgggc
     7861 aggctgcgca gcttgttctg cagtaccgca tcggcccgca gctgctcgcc gaactccagc
     7921 agggtgacgt gggcgacgat gccggccagg tcgatggccg cctcgacgcc ggagttgccg
     7981 ccgccaatca ccgccacgcg cttgcccttg aacagcgggc cgtcacagtg cgggcagtag
     8041 gccacgccgc ggccgcggta ttcctgctcg cccggcacgt tcatctctct ccagcgggcg
     8101 ccggtggcga cgatcacggt cttggccttc agcgtggcgc cgttgtcgaa ctgcacttca
     8161 tgcagaccgc cttcgctggc cgccggcacc agcttggcgg cgcgctgcag gttcatgatg
     8221 tcgacgtcgt actgcctgac gtgctcttcc agggcgcggg ccagcttcgg gccttcggtc
     8281 tccggcaccg agatgaagtt ctcgatggcc atggtgtcca gcacctggcc gccgaagcgc
     8341 tcggcggcga caccggtacg gatgcccttg cgggcggcgt agatcgccgc ggtggcgccg
     8401 gccgggccgc cgccgaccac cagcacgtcg aaggcgtcct tggcgttgag cttctcggca
     8461 tcgcgggcgc tggcgccggt gtcgagcttg gcgagaatct cttcttcgcc catgcggccc
     8521 tggccgaaca gttcgccgtt gaggtagatg ctcggcaccg acatgatctg gcgttgctcg
     8581 acctcatcct ggaacagcgc gccgtcgatg gcgacgtggc ggatgttggg gttgagcacg
     8641 gccatcaggt tcagcgcctg caccacgtcg gggcagttct ggcaggacag cgagaaatag
     8701 gtctcgaagt tgaattcgcc ttcgaggttc ttgatctgtt cgatcacgtc cgcgctggtc
     8761 ttcgacgggt ggccgccgac ctgcagcagg gccagcacca gcgaggtgaa ttcgtggccc
     8821 atcgggatgc cggcgaagcg caggctgata tcgccattga tgcggttcag ggcaaacgag
     8881 ggcttgcggc tatcgtcgcc gtcggtgcgc agggcgattt tgtcgctcag gctggcgatg
     8941 tcggtcagca ggccgagcag ttcctgggat ttcgcgccgt catcgaggga cgcgacgatc
     9001 tcgaacggct gggtgacctt ctcgaggtag gccttcaact gggttttcag attggcgtcc
     9061 aacatggcaa cgatatcctg tgcaaaattt gggtaataaa acgcccgggc ggatcgcgcc
     9121 cgggcgttcg ggcgcatgga gagccgggtc gcggctctcg ctgcgcggca accgcagggt
     9181 tgcttagatc ttgccgacca gatccaggga cggagtcagg gtttcggcac cttccttcca
     9241 cttggccggg cagacctggc ccgggtgggc ggcggtgaac tgggcggcct tcagcttgcg
     9301 cagggtctcg ctgacgtcac gggcgatctc gttggagtgg atttccaggg tcttgatcac
     9361 gccttccggg ttgatgatga aggtgccacg cagggccagg ccttcttccg gaatgtgcac
     9421 gccgaaggcg ttggtcagcg cgtgggtcgg gtcgccgatc agcgggaact gggccttgcc
     9481 gacagccggg gaggtttcgt gccagacctt gtgggagaag tgggtgtcgg tggtgacgat
     9541 gtacacctcg gcgccggcct tctggaattc ggcgtagttt tcggcggcgt cttcgatctc
     9601 ggtcgggcag ttgaaggtga aggctgccgg catgaagatc atgaccgacc acttgccctt
     9661 cagggtctgc tcggtgacct cgatgaactt gccgttgtgg aaagcgttga ccttgaacgg
     9721 ttgaacctgg gtgttgatga gggacattcg ctgaactcct tctgggggtt ggaagcttgc
     9781 ttggtgaagt gaatcctacc cggggtacgg ggttaggcat aattgattga tgaaatcaat
     9841 gtgattactt atgtctatga ttcgcttcgc cgccatccgt ccggaggccg gacggtgacg
     9901 gcctgctact ctacaacagg cttggccgta gggaaccttt gccgtacact ccgtctagac
     9961 ctagcaagca gaagtacctt ggggccgatc aggattcgac gccggtagca aagcttgagg
    10021 ggcatgccga gttggtaaca gaactcgtaa atccactgtt gcaaacttat agttgccaac
    10081 gacgacaact acgctctagc cgcctaaggg ccgctagctg accctagggg atgcctgtaa
    10141 acccgaagat ttggtcagtc atatagaaca ggatcgccgc caagttcgct gcagacgtaa
    10201 cggctaaaac tcatgcagct cgctccaagc accctgccag tcgggtcgcg aggagttaac
    10261 tcaatagaca tggctaagca tgtagaaccg atagtggaga gctggcggac gggggttcaa
    10321 atccccccgg ctccaccaa
//
LOCUS       DATCUN010000264         4315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_67791, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000264 DATCUN010000000
VERSION     DATCUN010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67791"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VIO83_10365"
     CDS             <1..378
                     /locus_tag="VIO83_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003227206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEY8331715.1"
                     /translation="HSAAWRGLGLDAENLLPPARSERLAGEILTPAELQHFQQLADEQ
                     RATYLTLCFSLKESLFKALYPLVRRRFYFQAAALVEQADNGRVRLQLREDLDAEWTRG
                     TCLEGQFGIREERLLSLVWVPAR"
     gene            985..3840
                     /locus_tag="VIO83_10370"
     CDS             985..3840
                     /locus_tag="VIO83_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEY8331716.1"
                     /translation="MQTDTTRENPTASQDLAATAPGQLRVIKRNGTVVPYTDDKITVA
                     ITKAFLAVEGGNAALSSRIHDTVARLTEQVTATFKRRMPSGGTIHIEEIQDQVELALM
                     RAGEQKVARDYVIYREARAQERKRSAGSEVAQPHPSIRITLANGEKIPLDLGRLQTII
                     AEACEGLAEVDGALIQRDTLKNLYDGVSEQDVNTALVMTARTLVEREPNYSYVTARLL
                     MDTLRAEALGFLGVAERATHHEMAELYAQALPVYIDKGIEFELLDPKLRDYDLAKLGA
                     ALDHERDQQFTYLGLQTLYDRYFIHKNDIRFELPQVFFMRVAMGLAIAEKDKEARAIE
                     FYNLLSSFDYMASTPTLFNAGTLRPQLSSCYLTTVPDDLGGIYDAIRDNALLSKFAGG
                     LGNDWTPVRALGSYIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCAYLETWHL
                     DIEEFLELRKNTGDDRRRTHDMNTANWIPDLFMKRVFDDGQWTLFSPSDVPDLHDLTG
                     KAFEERYEYYEAMAGYGKLKLHKTIAAKDLWRKMLSMLFETGHPWLTFKDPCNLRSPQ
                     QHVGVVHSSNLCTEITLNTNKDEIAVCNLGSVNLVNHIVDGKLDSAKLARTVKTAVRM
                     LDNVIDINYYSVPQAQNANFKHRPVGLGIMGFQDALYLQHIAYGSDAAVEFADQSMEA
                     ISYYAIQASCDLADERGAYSSFDGSLWSKGILPLDSQQILIEARGQKYIDVDLTESLD
                     WAPLRARVQKGIRNSNIMAIAPTATISNIVGVSQSIEPTYQNLYVKSNLSGEFTVINP
                     YLVHDLKARGLWDPVMVNDLKYYDGSVQQIERIPQDLKDLYATAFEVETRWIVEAASR
                     RQKWIDQAQSLNLYIAGASGKKLDVTYRMAWYRGLKTTYYLRALAATSTEKSTITTGK
                     LNAVSSVIDESLSAAEPKPAPVPQACSIDNPDCEACQ"
     gene            4163..>4315
                     /locus_tag="VIO83_10375"
     CDS             4163..>4315
                     /locus_tag="VIO83_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEY8331717.1"
                     /translation="MLSWDEFDTEEETVAKPAAAKAAASAAPDKLDNAAEAAVHDARA
                     VSADDSA"
BASE COUNT          872 a         1581 c         1213 g          649 t
ORIGIN      
        1 cacagcgccg cctggcgcgg cctcggcctc gacgcggaaa acctgctgcc gcccgcgcgc
       61 agcgagcgcc tggccggcga aatcctcacc cccgccgagc tgcagcactt ccagcaactg
      121 gccgacgagc aacgcgccac ctacctgacc ctgtgcttct cgctcaagga aagcctgttc
      181 aaggcgctct acccgctggt ccggcggcgc ttctacttcc aggccgccgc cctggtcgaa
      241 caggcggaca acggccgggt acgcctgcaa ctgcgcgagg atctggacgc cgaatggacc
      301 cggggcacct gcctggaagg gcagttcggc atccgcgaag agcgcctgct cagcctggtg
      361 tgggtccccg cccgctgaac cgtccgagca caatcgggac gtttttccaa gcctgccaac
      421 ccactagata ttgtgttaac ttgcgcgccc ttcgcagccc ggccagcccc ttctggagcg
      481 actgaaaagg cgacgaacgg tgcaagtaca gcatggctgc gggctgcgcc agggcagccc
      541 ggaaagcaca caggccaccc acaccttatc cacagcccta tccacagcca ccgtttgcac
      601 gaccgccacc atcgcactat gctgtagcag ccgaatgcac cgagcactac atgttgtggt
      661 tgctgaattt tttctgcgta cagaacgcca gctctttgac cgtcttcaca tggcgactga
      721 gaccctgttg cgatctccgc caagccaggc ttgctaggct ctcccaatgc gcagagctgg
      781 caaaaaacgc cgctgaagac taaacactat atctagtgcc ctgcctgcag gatgacccgc
      841 tcatctgccg tcgcagggcc agcgtaaaaa acgacgtttg atcggtaccg ccgcctccgc
      901 ctgccccatg tggtgttgtg gcatccgcct gcagaggcgg caccccgagc gtccaaaccg
      961 aaaaccgaaa ttgcggagcc ctcgatgcaa actgacacca ctcgcgagaa cccgacagcc
     1021 agccaggacc tagcggccac cgccccgggc cagctgcgcg tgatcaagcg caacggcact
     1081 gtcgtcccct acaccgacga caagatcacc gtggccatca ccaaggcctt cctcgcagtg
     1141 gaaggcggca acgccgccct ctcgtcgcgc atccacgaca ccgtcgcgcg cctgaccgaa
     1201 caggtcaccg ccaccttcaa gcgtcgcatg ccctccggcg gcaccatcca catcgaggaa
     1261 atccaggacc aggtcgaact ggccctgatg cgcgccggcg agcagaaggt cgcccgcgac
     1321 tacgtgatct accgcgaagc ccgcgcccag gagcgcaagc gcagcgccgg cagcgaagtg
     1381 gcccagccgc acccgagcat ccgcatcacc ctggccaacg gcgagaagat cccgctcgac
     1441 ctcggccgcc tgcagaccat catcgccgag gcctgcgaag gcctggccga agtggacggc
     1501 gcgctgatcc agcgcgacac cctgaagaac ctctacgacg gcgtctccga gcaggacgtc
     1561 aacaccgccc tggtgatgac cgcgcgcacc ctggtcgagc gcgagccgaa ctactcctac
     1621 gtcaccgccc gcctgctgat ggacaccctg cgcgccgaag cgctgggctt cctcggcgtc
     1681 gccgagcgcg ccacacacca cgagatggcc gagctgtacg cccaggccct gccggtgtac
     1741 atcgacaagg gcatcgagtt cgaactgctc gatcccaagc tgcgcgacta cgacctggcc
     1801 aagctgggcg ccgcgctgga tcacgagcgt gaccagcagt tcacctacct cggcctgcag
     1861 accctgtacg accgctactt catccacaag aacgacatcc gcttcgaact gccgcaggtg
     1921 ttcttcatgc gcgtggccat gggcctggcc atcgccgaaa aggacaaaga agcgcgcgcc
     1981 atcgagttct acaacctgct gtcctcgttc gactacatgg cgtcgacccc gaccctgttc
     2041 aacgccggca ccctgcgccc gcagctgtcc agctgctacc tgaccaccgt gccggacgac
     2101 ctgggcggca tctacgacgc catccgcgac aacgccctgc tgtccaagtt tgccggcggt
     2161 ctgggcaacg actggacccc ggtgcgcgca ctgggctcct acatcaaggg caccaacggc
     2221 aagagccagg gcgtcgtgcc gttcctgaaa gtggtcaacg acaccgccgt cgcggtcaac
     2281 cagggcggca agcgcaaggg cgcggtctgc gcctacctgg aaacctggca cctggacatc
     2341 gaggaattcc tcgagctgcg caagaacacc ggtgacgacc gtcgccgcac ccacgacatg
     2401 aacaccgcga actggattcc cgacctgttc atgaagcgcg tgttcgacga cggccagtgg
     2461 accctgttct cgccgtccga cgtgcccgac ctgcacgacc tgaccggcaa ggccttcgaa
     2521 gagcgctacg agtactacga agccatggcc ggctacggca agctcaagct gcacaagacc
     2581 atcgccgcca aggacctgtg gcgcaagatg ctgtccatgc tgttcgagac cggccacccg
     2641 tggctgacct tcaaggaccc atgcaacctg cgcagcccgc agcagcacgt cggcgtggta
     2701 cacagctcca acctgtgcac cgagatcacc ctgaacacca acaaggacga gatcgccgtc
     2761 tgcaacctgg gttcggtcaa cctggtcaac cacatcgtcg acggcaagct ggacagcgcc
     2821 aagctggccc gcaccgtgaa gaccgccgtg cgcatgctcg ataacgtcat cgacatcaac
     2881 tactactcgg tgccgcaggc gcagaacgcc aacttcaagc accgtccggt cggcctcggc
     2941 atcatgggct tccaggacgc gctgtacctg cagcacatcg cctacggctc cgacgctgcc
     3001 gtcgagttcg ccgaccagtc catggaagcc atcagctact acgcgatcca ggcttcctgc
     3061 gacctggccg acgagcgtgg cgcctactcc agcttcgacg gctcgctgtg gtccaagggc
     3121 atcctgccgc tggactccca gcagatcctc atcgaggccc gcggccagaa gtacatcgac
     3181 gtcgacctga ccgagtcgct ggactgggcg ccgctgcgtg cccgcgtgca gaaaggcatc
     3241 cgcaactcga acatcatggc catcgcgccg accgcgacca tctccaacat cgtgggcgtg
     3301 tcgcagtcga tcgagccgac ctaccagaac ctgtacgtga agtcgaacct gtccggcgaa
     3361 ttcaccgtga tcaaccccta cctggtccac gacctcaagg cccgcggcct gtgggacccg
     3421 gtgatggtca acgacctcaa gtactacgac ggctccgtgc agcagatcga gcgcatcccg
     3481 caagacctca aggacctgta cgccaccgcc ttcgaagtgg aaacccgctg gatcgtcgaa
     3541 gccgccagcc gccgccagaa gtggatcgac caggcccagt cgctcaacct gtacatcgcc
     3601 ggcgcctcgg gcaagaagct cgacgtgacc taccgcatgg cctggtaccg cggcctgaag
     3661 accacctact acctccgtgc cctggccgcg accagcaccg agaagtcgac catcaccacc
     3721 ggcaagctca acgccgtgtc gtcggtgatc gacgaaagcc tctcggctgc cgagcccaag
     3781 ccggctccgg tcccgcaggc gtgcagcatc gacaacccgg actgcgaagc ctgtcagtga
     3841 caggtgacgc acgaatctga gcggcgtccg cgttgttgcc ggctcgcccg ccccggtcac
     3901 gtactccagt acgctcccga gggcgagcaa tccggcacct agcggtcact cgctcagctt
     3961 cgcgctcgtt ttccagtcgt gattactccc cctcgccttt gcggcgaggg tcagggagag
     4021 gggcaaacca ggccaggccc tccccagacc aacggccccg ttttcagtct tgcgcacacg
     4081 ccgcaacgtc atagaatcaa tttactgtct atccatacag gccgatcagc gtcggcccgc
     4141 tcaaccaggc aggagaacca tcatgctgag ctgggatgaa ttcgataccg aagaagaaac
     4201 cgtagccaag ccggccgcgg ccaaggctgc cgccagcgct gccccggaca agctcgacaa
     4261 cgccgccgag gccgccgtgc acgacgcccg cgccgtctcc gccgacgact ccgcc
//
LOCUS       DATCUN010000265         3482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_67796, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000265 DATCUN010000000
VERSION     DATCUN010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67796"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VIO83_10380"
     CDS             complement(<1..351)
                     /locus_tag="VIO83_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEY8331718.1"
                     /translation="MSERIPAKIVETIEPRPTRLTPAQAGGPIHTRSFTGLFRNLRLY
                     GAGALFLLFFGTAWLNWGDRQAVLWDLAEHQFYIFGATFWPQDFILLSALLIICAFGL
                     FAITVAAGRVWCGYT"
     gene            complement(812..1690)
                     /locus_tag="VIO83_10385"
     CDS             complement(812..1690)
                     /locus_tag="VIO83_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015270273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8331719.1"
                     /translation="MSPQKTAFVLAGGGSLGAVQVGMLKALSRAQITPDLVVGASVGA
                     INAAYYAAAPDERGVACLESIWLGLHRADVFPLSTTSTLMCLLGKRDFLTLPGKLQAL
                     IETRLPYRDLEDAALPCHVVATDALDGTEVILSSGAACQALLASVAIPAVFPAVVIHG
                     RPLMDGGIASNTPISAAVALGATRVVILPTGTPCALQAPPHGALAIALHAVNLLAMRQ
                     LLADVDRFAGRCELIVLPPLCPLAINTFDFSHSAELIHRADKAAGRWLEDGLQGLQGK
                     DPRWLLAPHRHNPRQG"
     gene            complement(1709..2926)
                     /locus_tag="VIO83_10390"
     CDS             complement(1709..2926)
                     /locus_tag="VIO83_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331720.1"
                     /translation="MKSLADHPTTSEKTISDPGDFSLVLGGPLFQLLRRSRLAGDALE
                     LVQKRIVFIALLAWLPLLLLSLVEGLIRDGAVSIPFLMDFEVHIRFLVAMPLLIGAEL
                     IVHQRIRPVIGQFLERRLIPDDARPRFDAAIASAMRLRNSVTAEALIIAFVYGVGVLV
                     IWRHYAVLDAASWYTLPTDSGFELSLAGIWYGYVSVPVFQFLLVRWFFRLFIWMRFLW
                     QVSRIELNLVPTHPDRVGGLGFLANSAYAFMPLALAHGALLAGMLANRVLYLGAALPD
                     FKAEIALVVIFIELLVLAPLFVFVAQLANAKRIGLREYGGLAMTYVRDFDSKWLRGGA
                     PADEPLVGSGDIQSLADLGNSFAVVQEMRLAPVTRDVIMRLAFATLLPVVPLLLTIMP
                     LEELLKKLVGIVL"
     gene            complement(2996..>3482)
                     /locus_tag="VIO83_10395"
     CDS             complement(2996..>3482)
                     /locus_tag="VIO83_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEY8331721.1"
                     /translation="ASIPLFIRGLENLSAILHKAASHAEAKQIAPDVFLNARLAPDMF
                     PLLRQVQIVSDTAKGCAARLAGVEVPSYPDIETSFAELQERIAKTVTFLKDLAPAQVD
                     GSEERPITLKMHTQEVHFTGQAYLLGFALPNFYFHLTTAYGILRHNGVDIGKKDYLGE
                     V"
BASE COUNT          672 a         1054 c         1189 g          567 t
ORIGIN      
        1 ggtatagccg caccacacgc ggccggcggc cacggtgatg gcgaacaggc cgaaggcgca
       61 gatgatcagc agcgccgaga ggaggatgaa gtcctgcggc cagaaggtgg cgccgaagat
      121 gtagaactgg tgctcggcca ggtcccagag cacggcctga cggtcgcccc agttcagcca
      181 ggcggtgccg aagaacagca ggaacagcgc cccggcgccg tacaggcgca ggttgcggaa
      241 caggccggtg aagctgcggg tgtgaatggg accgccggct tgcgcgggcg tcaggcgcgt
      301 cggccgcggc tcgatcgtct cgacgatctt ggcgggaatg cgttcgctca tggggatggc
      361 tccatcagtc ctacaaaaga tggggccaat gatccgcgca tggctgcagg ggaaacagac
      421 tcagatccgt cgataaaaag cggatcagat gtccagcatc cgccgtggat cggcgctcgg
      481 ccagggccgc gccagggctt tgatatggtt ttttagcggg atctgccgct atttttgtct
      541 gttgccgctg ggcatagccg ccaccctgat tgcccgctgc cgcgagggcg tgcgcggctg
      601 tcgccttgcc tgtggttgcg gggccgattc gctccttcgt cgttggccgc gcccttttca
      661 gcgcgcggct tcttgccgct ggactgtgcg cctcaatcca ataggttttg ggaatgggat
      721 gtcaaagtag tcggggaatg tggaccttat gaccggtgca ggaagtggtg cctcggcagt
      781 aacggggcag gccgaccgcg cgacttctgg atcaaccttg ccttgggtta tgcctgtgcg
      841 gcgccagcag ccagcgggga tccttgccct gcagtccctg caagccgtcc tcaagccacc
      901 ggccggcggc tttgtccgcg cggtggatga gctcggccga gtgcgagaag tcgaaggtgt
      961 tgatcgccag cgggcacaac ggcggcagca cgatcagttc gcagcggccg gcgaaacgat
     1021 cgacgtcggc cagcagctgg cgcatggcga gcaggttgac ggcatgcagc gcgatggcca
     1081 atgcgccgtg cggcggcgcc tgcagcgcac agggcgtgcc ggtcggcagg atcaccaccc
     1141 gggtggctcc cagggcaacc gccgcggaga tcggcgtgtt gctggcgatg ccgccatcca
     1201 tcagcggccg gccgtggatg accaccgctg gaaacacggc cgggatggcc acgctggcga
     1261 gcagcgcctg gcaggccgcg ccggaagaga ggatgacctc ggtgccatca agtgcatcgg
     1321 tggcgaccac atggcagggc agtgcggcgt cttccaggtc ccggtagggg agccgcgtct
     1381 cgatcagggc ttgcagtttg ccggggaggg tcaggaaatc gcgtttgccg agcaggcaca
     1441 tgagggtgct ggtcgtcgac agcgggaaga catcggcgcg gtgcagcccc agccagatgc
     1501 tctcgaggca tgcgacgccg cgttcatcgg gggccgcggc atagtaggcc gcattgatcg
     1561 cccccaccga ggcgccgacc accaggtcgg gggtgatctg cgcccggctc agcgccttga
     1621 gcatgccgac ctgcacggcg ccgaggctgc cgccaccggc cagtacgaac gcggtcttct
     1681 gcggactcat gtcaaggcgc ctcgatcgct agagaactat gcccaccagc tttttcagca
     1741 gttcttcgag cggcatgatg gtgagcagga gtggcaccac cggcaacagc gtcgcaaacg
     1801 cgaggcgcat gatgacatcc cgcgtgaccg gggcgaggcg catctcttgc accaccgcaa
     1861 agctgttgcc gaggtcggca agggactgga tatcgccact gcccaccagc ggctcgtcgg
     1921 cgggtgcacc accgcgcagc catttactgt cgaagtcgcg cacgtaagtc atcgccagac
     1981 cgccgtattc gcgcagaccg atccgcttgg cgttggcaag ctgggcgaca aagacgaaaa
     2041 gcggggcaag caccagaagc tcgatgaaaa tgaccacgag ggcgatctcg gccttgaagt
     2101 cgggaagcgc ggcgccgagg tagaggaccc ggttggccag catgccggcc agcagtgccc
     2161 cgtgcgccag cgcgagcggc atgaaggcat aggccgagtt ggcgagaaag cccaggccgc
     2221 cgacccggtc cgggtgggta gggaccaggt tcaactcgat acgcgacacc tgccagagaa
     2281 agcgcatcca gatgaacagc cggaagaacc agcgcaccaa caggaactgg aagaccggca
     2341 cactcacgta gccataccat atcccggcga gcgacagctc gaatcccgaa tcggtcggca
     2401 gggtgtacca cgaggccgca tcgagtaccg catagtggcg ccagatcacg agtacgccca
     2461 ccccatagac gaatgcgatg atcagcgcct cggcagtcac cgagttgcgc aagcgcatgg
     2521 cggaggcaat cgcggcatcg aaacgtggcc tggcgtcgtc cggaatcaac ctgcgctcga
     2581 ggaactgccc gatcaccggg cggatacgct ggtgaacgat cagttcggcg ccgatcaaca
     2641 acggcatcgc taccagaaac cggatatgaa cttcaaagtc catgagaaag ggtatggata
     2701 ccgcgccgtc ccgtatcagc ccttcaacca gcgagagcag cagcagcggc agccaggcca
     2761 gcagggcaat gaagacgatg cgtttttgca ccagttcgag cgcgtcgccg gccagtcgcg
     2821 agcggcgtaa cagctgaaat agcgggccac ccagcaccag cgagaagtcg cctggatcac
     2881 tgatggtttt ttcagacgtt gttgggtgat cggctaatga tttcattccc cctcccatgc
     2941 catggcgacg tcaatgtcct tgcttgcagg catagtcgcc tgcctgggca cgggttcaga
     3001 cctcgcccag ataatccttc ttgccgatgt ccaccccgtt atgccgcagg atgccgtagg
     3061 cggtggtcag gtggaaatag aagttgggga gggcaaagcc cagcagatag gcctgcccgg
     3121 tgaaatggac ttcctgggtg tgcatcttca gggtgatcgg gcgttcctcg ctgccatcga
     3181 cctgcgccgg cgcaaggtct ttcaggaagg tcacggtctt ggcgatgcgc tcctgcaatt
     3241 cggcaaagct ggtttcgatg tccggataac tcggcacctc gacgccggcc agccgcgcgg
     3301 cgcaaccctt ggcggtgtcg gaaacgatct gtacctggcg cagcagcggg aacatgtcgg
     3361 gcgcgagccg ggcgttgagg aagacgtccg gtgcgatctg cttcgcctcg gcatggctgg
     3421 cggccttgtg caggatggcc gacaggttct ccaggccgcg gatgaacagc gggatggagg
     3481 cg
//
LOCUS       DATCUN010000266        10500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_86540, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000266 DATCUN010000000
VERSION     DATCUN010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10500
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86540"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VIO83_10400"
     CDS             complement(<1..733)
                     /locus_tag="VIO83_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEY8331722.1"
                     /translation="MNLFPDLAYLPGQLHHPAVRDLAWTLLSPPLLAAVDGLRPRHPL
                     CASGWAAHPERLANWLHDLDRQPQALLLQTPGANGSRLGRYYEELWQFALGQAPGIRL
                     LAANLAIRDHGHTLGEMDLLLEDDEGLQHIELAVKFYLGPPQADGRASRDWLGPGRLD
                     RLDLKLEHLLQHQLPLSCRPEARELIQALSSRAPQASLWLGGYLFYPWPTPCSSPRSA
                     GTQHLRGRWVQRRDWVACLAAARPGR"
     gene            839..1726
                     /locus_tag="VIO83_10405"
     CDS             839..1726
                     /locus_tag="VIO83_10405"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEY8331723.1"
                     /translation="MDQFRNIGIIGRLGSSQVLDTIRRLKRFLIERHLHVILDESIAE
                     MLPGHGMQTCTRKLLGEICDLVIVVGGDGSLLGAARAMARHGTPVLGINRGSLGFLTD
                     IRPDELEARVSEVLAGRYLLESRFLLEAHVRRGEEAIGQSDALNDVVLHPGKSTRMIE
                     FELYIDGQFVCSQKADGLIVATPTGSTAYSLSAGGPIMHPKLDAIVIVPMYPHTLSSR
                     PIVVDGNSELKVVVASDMQIYPLVSCDGQNHFTCAPGDTLTIRKKAQKLKLIHPLEHN
                     YYEVCRTKLGWGSRLGGHE"
     gene            1729..2700
                     /locus_tag="VIO83_10410"
     CDS             1729..2700
                     /locus_tag="VIO83_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003217895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8331724.1"
                     /translation="MLDPARSYDLIGDVHGCARALERLLSGLGYRRLGGVWRHPERMA
                     LFLGDILDRGPHIREALHLVHDMVEAGEALCLMGNHEWYALGWTTPALPGSGQAFARE
                     HNPRHERLIRETLAQFEQYPGDWHDFLGWFQRLPLLADAGRFRLVHACWDAETIEQLR
                     RDHPDGRIDRAFVQQAGEPDSFAAHCMQRLLRGLDMPLPPGLTLTSHDGFIRTAFRTK
                     FWEDAPETYGDIVFQPDALPEEVANLPLSNAQKAALLRYGEDEPLLFVGHYWCNGTPA
                     PLRPNLACLDYSAVRRGKLVAYRLDQEARLEPSKFCWVEVKRLEADA"
     gene            2697..3563
                     /locus_tag="VIO83_10415"
     CDS             2697..3563
                     /locus_tag="VIO83_10415"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY8331725.1"
                     /translation="MSAIAALRLPLAEDLGAFVVLLQRLGVPHRVSEESGEQVLWVPD
                     ETLALRVRELYAEYPAGIAGSELPPAAPRAASAGRMLAGLRSLPVTALVLLATFAVAL
                     ASWFGDNLQLVGWLTFTDFRVEGDYAYFLPLADSLAAGQWWRPFSPMLVHFGVLHLAM
                     NSLWFWELGRRIEWRQGSLMLLGLTLLYSLVSNLAQYVFGGPAIFGGLSGVLYGLLGH
                     CWLFQRLAPNPHFRLPPGVVGMMLVWLLVCLSGLIGALGFGEIANAAHVGGLLIGCAT
                     GVLGGLLARRQR"
     gene            3593..3856
                     /locus_tag="VIO83_10420"
     CDS             3593..3856
                     /locus_tag="VIO83_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1315 family protein"
                     /protein_id="HEY8331726.1"
                     /translation="MASFAEMIENITPEIYQSLKLAVEIGKWPDGRKLTQEQKELTLQ
                     AMIAWEVQNLPEEERTGYMGPQECTSKSFEVPNILFKSSDTLH"
     gene            3856..4686
                     /locus_tag="VIO83_10425"
     CDS             3856..4686
                     /locus_tag="VIO83_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HEY8331727.1"
                     /translation="MSELGRGALTKMAVRLEAPVQYSLRLGDAEVAVNPLIGKTLRLE
                     FLGAIHCSHCGRKTKKSYAQGYCYPCMTKLAQCDSCIMSPEKCHYDAGTCREPSWGEQ
                     FCMADHVVYLANSSGVKVGITRATQLPTRWLDQGASQALPILRVATRLQSGLVEDLLR
                     SQVADRTNWRALLKGDAEPVDLPAVREQLFDTCQAGLEALQQRFGLQAIQPLPDASVV
                     EMRYPVAAYPAKIVSFDLDKNPVVEGTLQGIKGQYLIFDTGVINIRKYTAYQLAVQQL
                     "
     gene            4698..7355
                     /gene="pepN"
                     /locus_tag="VIO83_10430"
     CDS             4698..7355
                     /gene="pepN"
                     /locus_tag="VIO83_10430"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEY8331728.1"
                     /translation="MRSEQPKTVYLKDYRAPDYLIDETHLTFELFEDHTLVHAQLVMR
                     RNPAAGSGLPPLVLDGQQLELLSLAVDDVPLGEGDYQLDDSHLTLQPTRSEFVIDSSV
                     RIHPESNTALEGLYKSSGMFCTQCEAEGFRKITFYLDRPDVMSKFTTTVSAVQKDYPV
                     LLSNGNPIASGHEEDGRHWATWQDPFMKPAYLFALVAGDLWCIEDSFKTMSGRDVALR
                     IYVEPENIGKLQHGMDSLKKSMKWDEEVYGREYDLDIFMIVAVNDFNMGAMENKGLNI
                     FNSSCVLAHPETATDAAHQRVEAVVAHEYFHNWSGNRVTCRDWFQLSLKEGFTVFRDA
                     EFSADMNSRTVKRIEDVAFLRTHQFAEDGGPMAHPVRPDAYMEISNFYTLTIYEKGAE
                     VLRMIHTLLGAEAFRQGSDLYFARHDGQAVTCDDFIKAMEDASGVDLSQFKRWYTQSG
                     TPRLAVEEAYDPVAHSYSLTFRQSCPATPGQAEKQPFVIPVAMGLLDAHGNELPLRLA
                     GEAAADGTSRVLAVSEAEQRFTFVDVAHKPLPSLLRGFSAPVKLSFPYSRDQLLFLMQ
                     HDSDGFNRWEAGQQLAVQVLQELVDQHQRDEALALDARLITALRSVLADESLDAAMVA
                     EMLSLPAEAYLAELSEVADVDAIHAAREFARQEISRELFDLLWARYQACREASRNTPY
                     AADAAQIARRSLQNIALSYLMLAGSDEVVDVCEDQFQHTDNMTERLAALAVLVNSPFE
                     SEKATALAKFADYFKDDALVMDQWFSVQAGSPLPGGLERVEALMHHPAFTLKNPNKVR
                     ALIGAFANQNHVNFHRLDGAGYRFLADQIITLNVLNPQIAARQLTPLTRWRKFDSTRQ
                     ALMRGELERILASGELSSDVFEVVSKSLV"
     gene            complement(7428..8318)
                     /locus_tag="VIO83_10435"
     CDS             complement(7428..8318)
                     /locus_tag="VIO83_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8331729.1"
                     /translation="MDRLQAMQLFVRIVELGSFSKAAEQLAMSRASATALVKQLESHL
                     GARLLQRTTRQVSATLDGRSYYQHCLSILAEIEEAESVFSRSAQHPRGHLKVDLPASL
                     GRLVVIPALPEFYARYPEISLEIGIGDRMIDLVREGVDCVVRIGALDDSTLVARALPP
                     LRQLTCASAGYVARHGLPTSLDQLEGHQCVDYLSATSGRLQPLEFSVAGRVMPYPLPA
                     CLAVNNGESYVAACEAGLGIVQVPHYHVQRQLAAGTLVELLPQHRPPPLPMTVLYPHH
                     RHLTPRLRVFIDWLVELFGG"
     gene            8453..9676
                     /locus_tag="VIO83_10440"
     CDS             8453..9676
                     /locus_tag="VIO83_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEY8331730.1"
                     /translation="MKRSTIHQDFSISAVTAGFLAVLISYSGPLIIFFQAAQSAEVST
                     ALMTSWVWAISVGAALSGILLSWWLKVPVVTAWSAPGTALLVTLFPELSLNQAVGAYL
                     TAGVVIFLIGVSGWFDRIVQAIPKGIACAMMAGILFQFGVGAFKAVESMPALALGMIA
                     AYLVLKRLFPRYCLVLLLIVGVALSVGLEDANLQGIELSLAAPLFIAPEWTWSATLSL
                     AIPLVLVSLTGQFLPGMAILRSSGYHTPAKPLIAVTGLASLAVACFGGITIVIAAITA
                     ALCTGKDAHESPDKRYVAGIANGVFYLLGGCFAGTIILLFTSMPKAFVAVLAGLALIG
                     AITSNVMGAVHEAEHREASMITFLATASGMSFFGLGSAFWGVALGSLAYLVLHKSGWL
                     PRKAPAVALGVPSKD"
     gene            9890..>10500
                     /locus_tag="VIO83_10445"
     CDS             9890..>10500
                     /locus_tag="VIO83_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8331731.1"
                     /translation="MSQKIAGKGFLALAVSAALGLSSVVQAEILIGVAGPHTGPSAAI
                     GEQYWRGASQAAADINAAGGINGEPIKLVKGDDACEPKQAVAVANRLVDQDKVAGVIG
                     HFCSSSTIPASAVYDEAAVLMITPGSTNPVVTDRRLNVVFRMCGRDDQQGQVAADFIV
                     DTLKGARVAIIHDKDTYGQGIANAAKAQLNKRGVTEVLYEGLTR"
BASE COUNT         1687 a         3622 c         3488 g         1703 t
ORIGIN      
        1 agcgaccggg ccgggccgcc gccaggcagg ccacccagtc gcggcgctgc acccagcggc
       61 cgcgcagatg ctgtgttccg gcactccgtg gcgagctgca gggcgtcggc caggggtaga
      121 acaggtagcc tcccagccac aggctggcct gcggcgcccg cgagctgagg gcctggatca
      181 gctcgcgggc ttcggggcgg caggagagtg gtagctgatg ctgcagcagg tgctccagct
      241 tgaggtcgag gcggtcgagg cggccgggac cgagccagtc gcgggaggca cggccatccg
      301 cctgcggcgg cccgagatag aacttcaccg ccagctcgat gtgctgcagg ccttcgtcat
      361 cctccagcag cagatccatc tcgccgaggg tgtggccgtg gtcgcggatc gccaggttgg
      421 ccgccagcag gcggatgccc ggggcctggc cgagggcgaa ctgccacagc tcctcgtagt
      481 agcgtcccag gcggctgccg ttggcgcccg gcgtttgcag cagcagcgcc tgcggctggc
      541 ggtcgagatc gtgcaaccag ttggccagtc gctctggatg cgccgcccag ccgctggcgc
      601 acagcggatg ccgcggacgc aggccatcca ccgcagccag cagcggcggg ctgagcaagg
      661 tccaggccag gtcgcgtacc gccggatgat gcagctggcc gggcaggtag gcgagatcgg
      721 ggaacaggtt catgccagcg agcatagccg ctggccgccg cgattgtcgc ggtttgcccg
      781 gcggccggcg cttcccccat aatcgagtct tccgcagact gcagccggca gaatctccat
      841 ggatcaattc cgcaatatcg gcatcatcgg gcgcttgggc agctcgcagg tgctggacac
      901 cattcgtcgt ctgaaacgct tcctgatcga gcgtcacctg catgtgattc ttgacgagag
      961 tatcgccgag atgctcccgg ggcacggcat gcagacgtgt acgcgcaagc tgctcggcga
     1021 gatctgtgat ctggtgatcg tggtcggcgg cgacggcagc ctgctcggcg ccgcccgcgc
     1081 catggcccgc cacggcactc cggtgctggg catcaaccgc ggcagcctgg gctttctcac
     1141 cgacatccgt cccgacgagc tggaggcccg ggtcagcgag gtgctcgccg gccgctatct
     1201 gctggagagc cgtttcctgc tcgaggcgca cgtgcgccgc ggcgaggaag ccatcggcca
     1261 gagcgatgcc ctcaacgatg tggtgctgca tccgggcaag tcgacgcgga tgatcgagtt
     1321 cgagctgtac atcgatggcc agttcgtctg cagccagaag gccgacggct tgatcgtcgc
     1381 cacgccgacc ggctccaccg cctattcgct gtcggcgggc ggaccgatca tgcatcccaa
     1441 gctggacgcc atcgtcatcg tgccgatgta cccgcacacc ctgtccagcc ggccgatcgt
     1501 ggtcgacggc aacagcgaac tgaaggtggt ggtggcctcg gacatgcaga tctatccgct
     1561 ggtgtcctgc gacgggcaga accacttcac ctgcgcgccc ggcgataccc tgaccatccg
     1621 caagaaggcg cagaagctga agctgatcca cccgctcgag cacaactact acgaggtctg
     1681 ccggaccaag ctgggttggg gcagtcgcct cggggggcac gagtagtgct gctcgacccg
     1741 gctcgcagct acgacctgat cggcgacgtg catggttgcg cgcgcgccct cgagcgcctg
     1801 ctgtccggtc tcggctaccg ccgtctgggc ggtgtctggc gccaccccga acgtatggcc
     1861 ctgttcctcg gcgacatcct cgaccgcggt ccgcacatcc gcgaggcgct gcacctggtg
     1921 catgacatgg tcgaggccgg cgaggccctg tgcctgatgg gcaaccacga gtggtacgcc
     1981 ctcggctgga ccacgccggc cttgccgggt agcggccagg cgttcgcccg cgagcacaac
     2041 ccgcgccacg agcggctaat ccgcgaaacc ctggcgcagt tcgagcaata cccgggtgac
     2101 tggcacgact tcctcggctg gttccagcgt ctgccactat tggccgatgc cgggcgcttc
     2161 cgcctggtgc atgcctgctg ggatgccgag acgatcgagc agctgcgccg cgatcacccg
     2221 gacgggcgca tcgaccgcgc cttcgtccag caggccggcg agcccgacag cttcgccgct
     2281 cactgcatgc agcgcctgct gcgcggcctc gacatgccgc tgccgcccgg cctgaccctg
     2341 accagccacg atggcttcat ccgtaccgcc ttccgcacca agttctggga ggacgccccg
     2401 gagacctatg gcgacatcgt gttccagccc gacgcgctgc ccgaggaggt ggccaacctg
     2461 ccgctgagca atgcgcagaa ggccgcgctg ctgcgctatg gcgaggacga gccgctgctg
     2521 ttcgtcggtc actactggtg caacggcacc ccggcgccgc tgcgccccaa cctggcctgc
     2581 ctcgactaca gcgcggtgcg ccgcggcaag ctggtggcct atcgcctgga tcaggaagcc
     2641 cgcctggagc ccagcaagtt ctgctgggtc gaggtcaagc gcctggaggc cgatgcatga
     2701 gtgcgattgc ggccctgcgt ctgcctctgg ctgaggatct gggcgccttc gtcgttctgc
     2761 tgcagcgcct cggcgtgccg caccgggtca gcgaggagtc cggcgaacag gtgctctggg
     2821 tgccggacga gacgctggcc ctgcgcgtgc gcgagctgta cgccgaatac ccggccggca
     2881 tcgccggcag cgagttgcca cctgccgcac cgcgtgcggc ctcggcgggg cggatgctgg
     2941 ctggcctgcg cagcctgccg gtgaccgcgc tggtgctgct ggcgaccttc gccgtggcgc
     3001 tggccagctg gttcggcgac aacctgcaac tggtcggctg gctgaccttc accgacttcc
     3061 gcgtcgaggg cgactacgcc tacttcctgc cgctggccga cagcctggcc gcgggccagt
     3121 ggtggcggcc gttcagcccg atgctggtgc actttggcgt gttgcacctg gcgatgaaca
     3181 gcctgtggtt ctgggagctg gggcggcgca tcgagtggcg ccagggcagc ctgatgctgc
     3241 tcggcttgac cctgctgtac agcctggtat ccaacctggc ccagtatgtc tttggcgggc
     3301 cggcgatctt cggcggcctg tccggcgtgc tctacggtct gctcggccac tgctggctgt
     3361 tccagcgtct ggcgcccaat ccgcacttcc gcctgccgcc cggtgtggtc ggcatgatgc
     3421 tggtctggct gctggtctgc ctgagcgggc tgatcggcgc gctgggcttt ggcgagatcg
     3481 ccaacgccgc ccatgtcggc ggcctgctga tcggctgcgc gaccggtgtg ctcggcggcc
     3541 tgctggcgcg ccgacagcgt taaactcaaa ctttcttcct gggagacacg ccatggcgtc
     3601 ctttgccgaa atgatcgaga acatcacccc cgagatctac cagagcctca agctcgccgt
     3661 ggagatcggc aagtggccgg acggccgcaa gctcacccaa gagcagaagg aactgaccct
     3721 gcaggcgatg atcgcctggg aggtgcagaa cctgccggaa gaagagcgca ccggttacat
     3781 gggcccgcag gagtgcacct cgaagtcctt cgaagtgccg aacatcctgt tcaagtcctc
     3841 ggacaccctg cactgatgag cgaactcgga cgtggcgcgc tgaccaagat ggcggtgcgc
     3901 ctggaggccc cggtgcagta cagcctgcgc ctgggggacg ccgaggtggc ggtcaacccg
     3961 ctgatcggca agaccctgcg cctggagttc ctcggcgcca tccattgcag ccactgcggc
     4021 aggaagacca agaagagcta cgcccagggc tactgctatc cgtgcatgac caagctggcg
     4081 cagtgcgaca gctgcattat gagcccggaa aagtgccact acgacgccgg cacctgccgc
     4141 gagccgagtt ggggcgagca gttctgcatg gccgatcatg tggtctacct ggccaactcc
     4201 tccggggtga aggtcggcat cacccgcgcc acccagctgc ccacccgctg gctcgaccag
     4261 ggcgccagcc aggcgctgcc gatcctgcgc gtggccaccc gcctgcagtc cggcctggtc
     4321 gaagacctgc tgcgcagcca ggtggccgac cgcaccaact ggcgcgcgct gctcaagggc
     4381 gacgccgaac cggtcgatct accggcggtg cgcgagcaac tgttcgatac gtgccaggcc
     4441 ggcctcgagg cgctgcagca gcgcttcggc ctgcaggcga tccagccgct gcccgacgcc
     4501 tcggttgtag agatgcgcta tccggtcgcg gcctatccgg ccaagatcgt cagcttcgat
     4561 ctggacaaga acccggtggt cgagggcaca ctgcagggca tcaagggcca gtacctgatc
     4621 ttcgataccg gcgtgatcaa cattcgcaag tacacggcct atcagctggc cgtgcagcaa
     4681 ctctaaggaa gccagacatg cgcagcgaac aaccgaagac tgtctatctc aaggactatc
     4741 gggcccccga ctacctgatc gacgagaccc acctgacctt cgaactgttc gaggaccaca
     4801 ccctggtcca tgcgcaactg gtcatgcgcc gcaacccggc ggccggcagc ggcctgccgc
     4861 cgctggtgct ggacggccag cagctcgagc tgctctcctt ggcggtggac gacgtgccgc
     4921 tgggcgaggg cgactaccag ctcgacgaca gccacctgac cctgcagccg acccgcagcg
     4981 agttcgtcat cgacagcagc gtgcgcatcc acccggaaag caacaccgcg ctggaaggcc
     5041 tgtacaagtc cagcggcatg ttctgcaccc agtgcgaggc cgagggcttc cgcaagatca
     5101 ccttctacct cgaccgcccg gacgtgatga gcaagttcac caccaccgtc agcgcggtgc
     5161 agaaggacta cccggtgctg ctctccaacg gcaacccgat cgccagcggc cacgaagagg
     5221 acggccgcca ctgggccacc tggcaggacc cgttcatgaa gccggcctac ctgttcgccc
     5281 tggtcgccgg cgacctgtgg tgcatcgagg acagcttcaa gaccatgagc ggccgcgacg
     5341 tcgccctgcg catctacgtc gagccggaga acatcggcaa gctgcagcac ggcatggaca
     5401 gcctgaagaa gtcgatgaag tgggacgagg aggtctacgg ccgcgaatac gacctggaca
     5461 tcttcatgat cgtcgcggtc aacgacttca acatgggcgc catggagaac aagggcctca
     5521 acatcttcaa ctccagctgc gtgctggccc atccggaaac cgccactgac gccgcccacc
     5581 agcgcgtcga ggcagtggtc gcccacgaat acttccacaa ctggtcgggc aaccgggtga
     5641 cctgccggga ctggttccag ctgtcgctga aggaaggctt caccgtgttc cgcgacgccg
     5701 agttttcggc cgacatgaac tcgcgcacgg tcaagcgcat cgaggacgtc gccttcctgc
     5761 gcacccacca gttcgccgag gatggcggtc ccatggccca cccggtgcgc ccggacgcct
     5821 acatggagat ctccaacttc tacaccctga ccatctacga gaagggcgcg gaagtgctgc
     5881 gcatgatcca caccctgctc ggcgccgagg ccttccgcca gggcagcgac ctgtacttcg
     5941 cgcgccacga tggccaggcg gtgacctgcg acgacttcat caaggccatg gaggacgcca
     6001 gcggcgtcga tctctcccag ttcaagcgct ggtacaccca gtccggcacc ccgcgcctgg
     6061 cggtggagga ggcctacgac ccggtcgccc atagctacag cctgaccttc cggcagagct
     6121 gcccggccac gccggggcag gccgagaagc agcccttcgt catcccggtg gccatgggcc
     6181 tgctcgacgc ccacggcaac gaactgccgc tgcgcctggc cggtgaggcc gcggcggacg
     6241 gcacctcccg cgtgctggcg gtgagcgagg ccgagcagcg cttcaccttc gtcgatgtcg
     6301 cccacaagcc gctgccctcg ctgctgcgcg gcttctccgc gccggtcaag ctgagcttcc
     6361 cctacagccg cgaccagctg ctgttcctca tgcagcacga ctccgacggc ttcaaccgct
     6421 gggaagccgg ccagcagctc gccgtgcagg tgctgcagga actggtcgac cagcaccagc
     6481 gcgacgaggc gctggccctc gatgcccgcc tgatcacggc gctgcgcagc gtgctggccg
     6541 atgaaagcct ggatgcggcg atggtcgccg agatgctctc gctgccggcc gaggcctacc
     6601 tggccgagct gagcgaagtg gccgacgtcg acgccatcca cgccgcccgc gaattcgccc
     6661 gccaggagat ctcccgcgag ctgttcgacc tgctgtgggc gcgctaccag gcctgccgcg
     6721 aagcctcgcg caacacgccc tacgccgccg acgccgcaca gatcgcccgg cgcagcctgc
     6781 agaacatcgc gctgtcctac ctgatgctgg ccggcagcga cgaggtggtc gacgtctgcg
     6841 aggaccagtt ccagcacacc gacaacatga ccgagcgcct ggccgcgctg gccgtgctgg
     6901 tcaactcgcc gttcgagtcg gagaaggcga cggccctggc caagttcgcc gactacttca
     6961 aggacgacgc cctggtcatg gaccagtggt tcagcgtgca ggccggcagc ccgctgccgg
     7021 gcggcctgga gcgggtcgag gcgctgatgc accacccggc cttcaccctg aagaacccca
     7081 acaaggtgcg tgcgctgatc ggcgccttcg ccaaccagaa ccacgtcaac ttccaccgcc
     7141 tcgacggtgc tggctatcgc ttcctcgccg accagatcat caccctcaac gtcctcaacc
     7201 cgcagatcgc cgcccgccag ctgaccccgc tgacccgctg gcgcaagttc gacagcaccc
     7261 gccaggcgct gatgcgcggc gagctggagc gcatcctggc cagcggcgag ctgtccagcg
     7321 atgtgttcga ggtggtgagc aagagcctgg tctgatccgt ccagggcagg tcaaggccgc
     7381 gtcggcactg ctgtgtcggc gcggccttgg cgtttccggg gtatggctca gccgccgaat
     7441 agctccacca gccagtcgat gaacacccgc aggcgcgggg tcaggtggcg gtggtggggg
     7501 tagagcacgg tcatcggcag cggcggcggg cggtgctggg gcagcagttc gaccaaggtc
     7561 ccggcggcca gctggcgctg cacgtggtag tgcggcacct ggacgatgcc caggcccgcc
     7621 tcgcaggccg ccacgtagga ctcgccgttg ttgaccgcca ggcaggcggg cagggggtag
     7681 ggcatgaccc gcccagcgac actgaactcc agcggctgca ggcggccact ggtggccgac
     7741 aggtagtcga cgcactggtg cccttcgagc tggtcgaggc tggtcggcag gccgtggcgg
     7801 gccacatagc cggcgctggc gcaggtcagc tggcgcagcg gcggcagggc gcgggcgacc
     7861 agggtggaat cgtccagcgc gccgatgcgc accacgcaat ccactccttc gcggaccagg
     7921 tcgatcatcc ggtcgccgat gccgatttcc aggctgatct ccgggtagcg cgcgtagaac
     7981 tccggcagcg ccgggatcac caccagccga ccgagcgagg cgggcaggtc gaccttcagg
     8041 tggccgcgcg ggtgctgcgc gctgcgcgaa aacacgcttt ccgcttcctc gatctcggcg
     8101 aggatgctca ggcagtgctg gtagtaggag cgcccgtcga gggtggcgct gacctggcgg
     8161 gtggtgcgct gcagcaggcg cgcgcccaga tggctttcca gctgcttgac cagtgcggtc
     8221 gccgaggccc gcgacatggc gagctgttcg gccgccttgc tgaagctgcc gagttcgacg
     8281 atgcgcacga acagctgcat ggcctgcaaa cggtccatag ttgctccgat tgttcttgac
     8341 ttccgaacag tgttgccagt tttgccggct ttatcaattc aaggactgac aataaggttg
     8401 cagcatcgcc ggccccatgc gccggccggc atgcttggcg ggagcaccca gaatgaagcg
     8461 cagcacgatc caccaggatt tttcgatttc ggccgtcacg gccggctttc tcgccgtact
     8521 gatctcctat tcagggccgt tgatcatctt cttccaggcc gcgcagagcg ccgaggtttc
     8581 cacggcgctg atgacctcct gggtctgggc catctccgtc ggcgcggcgc tgtccggcat
     8641 cctgctcagc tggtggctca aggtgccggt ggtgaccgcc tggtccgccc ccggcacggc
     8701 gctgctggtc accctgttcc ccgagctcag cctcaatcag gcggtgggcg cctatctcac
     8761 cgcgggcgtg gtcatcttcc tgatcggcgt ttccggctgg ttcgaccgga tcgtgcaggc
     8821 catccccaag ggcattgcct gcgcgatgat ggccggcatc ctgttccagt tcggcgtggg
     8881 cgcgttcaag gcggtggaaa gcatgccggc cctggcgctc ggcatgatcg ccgcctacct
     8941 ggtgctcaag cgcctgtttc cccgctattg cctggtgctg ctgctgatcg tcggcgtggc
     9001 cctgtccgtc ggcctggaag acgccaacct gcagggcatc gagctgagcc tggccgcacc
     9061 gctgttcatc gcaccggagt ggacctggag cgccaccctc agcctggcga tcccgctggt
     9121 gctggtcagc ctgaccggcc agttcctgcc gggcatggcg atcctgcgca gctcgggtta
     9181 ccacaccccg gccaagccgc tgatcgccgt taccggcctg gcctcgctgg cggtggcctg
     9241 cttcggcggc atcaccatcg tcatcgccgc catcaccgcg gccctgtgca ccggcaagga
     9301 tgcccatgag agtcccgaca agcgctatgt cgccgggatc gccaacggcg tgttctacct
     9361 gctgggcggc tgcttcgccg gcaccatcat cctgctgttc acctccatgc ccaaggcctt
     9421 cgtcgccgtg ctggccgggc tggcgctgat cggcgccatc accagcaacg tgatgggcgc
     9481 ggtacacgag gcggagcacc gcgaggcgtc gatgatcacc ttcctggcca ccgcctcggg
     9541 gatgagtttc ttcggcctgg gctcggcctt ctggggcgtg gcgctcggct cgctggccta
     9601 cctggtcctg cacaagtccg gctggctgcc gcgcaaggcc ccggcggtcg ctctgggggt
     9661 gccgagcaag gactgagccg gcgcggggtg acggcaagcg tgcatgcgcc gcgatgggga
     9721 tcgcttggcg aaatcagctc gtggcaccgg cggcatggcc cgccataggc taaaaagata
     9781 tgaggtggag tgcgttatgc cgattgcgca gaaggacggc atggcgcata ccttgccgga
     9841 cgcctgtcgc tcgccgggag cgaccatccc catctccagg agcaccttca tgtcgcagaa
     9901 gattgcagga aaaggctttc tcgccctcgc ggtgtccgcc gcgctgggcc tgtcttccgt
     9961 ggttcaggcc gagatactga tcggcgtggc cggcccccac accggcccca gcgcagccat
    10021 tggcgagcag tactggcgcg gggccagcca ggcggcggcc gacatcaatg ccgccggcgg
    10081 catcaacggc gagccgatca agctggtcaa gggcgacgac gcctgcgagc cgaagcaggc
    10141 ggtggcggtg gccaaccgcc tggtcgatca ggacaaggtg gccggggtga tcggccattt
    10201 ctgctcctcg tccaccatcc cggcctccgc ggtgtacgac gaggcggccg tgctgatgat
    10261 cacccccggt tcgaccaacc cggtggtcac cgaccgccgc ctcaacgtgg tgttccgcat
    10321 gtgcggccgc gacgaccagc agggccaggt cgccgccgac ttcatcgtcg acaccctgaa
    10381 gggcgcgcgg gtggcgatca tccacgacaa ggacacctac ggtcagggca tcgccaatgc
    10441 cgccaaggcc cagctcaaca agcgcggcgt caccgaggtg ctctacgagg ggctgacccg
//
LOCUS       DATCUN010000267         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_106080, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000267 DATCUN010000000
VERSION     DATCUN010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106080"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /gene="narH"
                     /locus_tag="VIO83_10450"
     CDS             <1..1351
                     /gene="narH"
                     /locus_tag="VIO83_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014339179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEY8331732.1"
                     /translation="QDKWNGGWVRSPDGSIAPRQGGKWKLLMRIFANPNLPQIDDYYE
                     PFTFDYDHLQSAPESKAAPTARPRSLITGQRMEKIEWGPNWEEILGGEFSKRSADKNF
                     DEVQKDIYGQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRG
                     WRMCVSGCPYKKIYYNWQSGKAEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDA
                     DRIQEAASVERDRDLYQAQLDIFLDPNDPDVIKQARIDGIPDSWMDAARNSPVYKMAV
                     QWKVALPLHPEYRTLPMVWYVPPLSPITAAANAGHVGVNGDIPDVSQLRIPVQYLANL
                     LTAGDTGPVVRALERMLAMRTYQRDKHVEQRQNLSVLKQAGLSMAEVEEMYHVMAIAN
                     YEDRFVIPSAHREYAENAFDIRGGCGFSFGNGCSDGATETSMFGGKKPRTIPIKATV"
     gene            1372..2073
                     /gene="narJ"
                     /locus_tag="VIO83_10455"
     CDS             1372..2073
                     /gene="narJ"
                     /locus_tag="VIO83_10455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEY8331733.1"
                     /translation="MFKKNPTPVRLTLRALGYLLSYPDAQLRSVMPQLIDALQAEQAL
                     TVERMDELKAVCEHLAALDPLEAEARYVDNFDRGRQTSLHLFEHVHGDSRDRGPALID
                     LLQTYEKAGLQFQADELPDHLPVVLEFASTQPPEVAKEFLGEMAHILNALFSALVARS
                     SPYASVIAAVLEVSGQRVQSVALTPEPPMDEVWAEPEAFGGCSTQGQAKPDQPQPLHF
                     VRTPRAERAPEGVSA"
     gene            2070..>2352
                     /gene="narI"
                     /locus_tag="VIO83_10460"
     CDS             2070..>2352
                     /gene="narI"
                     /locus_tag="VIO83_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEY8331734.1"
                     /translation="MTAWLDTLLFGIYPYICLAVFFIGSWARFDRDQYTWKSDSSQLL
                     RTGRLRWGSNLFHIGVLFLFFGHTVGMLTPHFLYEGFISAGNKQLMAMVT"
BASE COUNT          444 a          809 c          713 g          386 t
ORIGIN      
        1 ccaggacaag tggaatggcg gctgggtgcg cagccccgac ggctccatcg cgccccgcca
       61 gggcggcaag tggaagctgc tcatgcgcat cttcgccaac cccaacctgc cgcagatcga
      121 cgactactac gagcccttca ccttcgacta cgaccacctg cagtccgcgc ccgaaagcaa
      181 ggccgcgccc acggcgcgcc cgcgcagcct gatcaccggc cagcgcatgg aaaaaatcga
      241 gtggggcccg aactgggagg aaatcctggg cggcgaattc agcaagcgca gcgccgacaa
      301 gaacttcgac gaggtgcaaa aggacatcta cggccagttc gagaacacct tcatgatgta
      361 cctgccgcgc ctgtgcgagc actgcctgaa cccggcctgc gtggcctcgt gcccctcagg
      421 ctcgatctac aagcgcgagg aagacggcat cgtgctgatc gaccaggaca agtgccgcgg
      481 ctggcgcatg tgcgtctcgg gctgccccta caagaagatc tactacaact ggcagtcggg
      541 caaggccgag aaatgcatct tctgctaccc gcgcatcgag gccggccagc ccaccgtatg
      601 ctccgagacc tgcgtgggcc gcatccgtta cctgggcgtg ctgctgtacg acgccgaccg
      661 catccaggaa gccgccagcg tggagcgcga ccgcgacctg taccaggcgc agctcgacat
      721 cttcctggac ccgaacgacc cggacgtgat caagcaggcc cgcatcgacg gcattcccga
      781 cagctggatg gacgctgccc gcaacagccc cgtctacaag atggcagtgc aatggaaggt
      841 ggccctgccg ctgcaccccg agtaccgcac gctgcccatg gtctggtacg tgccgccact
      901 ctctcccatc acggccgccg ccaacgccgg ccatgtgggc gtgaatggcg acatccccga
      961 tgtttcgcaa ctgcgcatcc ccgtacagta cctggccaac ctgctcaccg ccggtgacac
     1021 cggacccgtg gtgcgggcgc tggagcgcat gctggccatg cgcacctacc agcgcgacaa
     1081 gcatgtggag cagcgccaga acctgtcggt gctcaagcag gccggcctgt cgatggccga
     1141 ggtcgaggag atgtaccacg tgatggcgat tgccaattac gaggaccgct tcgtcatccc
     1201 atcggcccac cgcgagtacg cagaaaacgc gttcgacatc cgcggcggtt gcggcttctc
     1261 gtttggcaac ggttgctccg acggcgccac cgagaccagt atgttcggtg gcaagaagcc
     1321 gcggaccatt cccatcaaag cgactgtttg agcgacacgg aaggaatcac catgttcaaa
     1381 aagaacccga cccccgtgcg cctgaccctg cgcgccctgg gctacctgct gagctacccc
     1441 gatgcgcagc tgcgcagcgt gatgccgcag ctcatcgatg ctctgcaggc cgagcaagcc
     1501 ctgacggtcg aacgcatgga tgaattgaag gcggtgtgcg agcacctggc ggcactcgac
     1561 ccgctcgaag ccgaagcccg ctacgtggac aacttcgatc gcggccgcca gacctcgctg
     1621 cacctgttcg agcatgtgca cggcgactcg cgcgaccggg gcccggcgct catcgatttg
     1681 ctgcagacct acgaaaaggc tggcctgcag ttccaggccg acgagctgcc cgaccacctg
     1741 cccgtggtgc tggagttcgc ctccacccag ccgcccgaag tggccaagga attcctgggc
     1801 gaaatggccc acatcctcaa cgccctgttc agcgcgctgg tggccagaag cagcccctat
     1861 gccagcgtga ttgccgcggt gctcgaagtg tcgggccagc gtgtgcagtc ggtggcgctc
     1921 acccccgagc cgcccatgga cgaggtctgg gccgagcccg aagcgtttgg cggctgcagc
     1981 acccagggcc aggccaagcc tgaccagccg caacccctgc attttgttcg taccccccgc
     2041 gccgagcggg cacccgaagg agtctcagca tgaccgcctg gcttgacacc ctgttgtttg
     2101 gcatctatcc ctatatttgc ctggccgttt tcttcattgg cagctgggcg cggtttgacc
     2161 gtgaccagta cacctggaag agcgattcgt cgcagctgct gcgcaccggc cgcctgcgct
     2221 ggggcagcaa cctgttccac attggcgtgc tgttcctgtt ctttggccac accgtgggca
     2281 tgctcacgcc gcacttcttg tacgagggtt tcatcagcgc gggcaacaag cagctgatgg
     2341 ccatggtgac gg
//
LOCUS       DATCUN010000268         3406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_218920, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000268 DATCUN010000000
VERSION     DATCUN010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218920"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /gene="dnaG"
                     /locus_tag="VIO83_10465"
     CDS             complement(<1..1528)
                     /gene="dnaG"
                     /locus_tag="VIO83_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY8331735.1"
                     /translation="MAGLIPQRFIDDLLNRTDIVEVVGSRIQLKKTGKNYSARCPFHN
                     EKTPSFSVSPDKQFYYCFGCGAGGNALGFIMDHDHLEFPEAVEELAKRAGLEVPHEDS
                     RPGQKPRQAVDSPLYPLLDAAAGFYRQALKSHPARQAAVDYLKGRGLSGVIARDFGLG
                     FAPPGWDNLMKHLGGDTLQQKAMIDAGLLIDNPETGKRYDRFRDRVMFPIRDSRGRVI
                     AFGGRVLGDDKPKYLNSPETPVFHKGQELYGLYEARQHHRDLDEVMVVEGYMDVIALA
                     QQGLRNAVATLGTATSEEHIKRLFRLVPNILFCFDGDQAGRKAAWRALEACLPSLQDG
                     RRVRFLFLPEGEDPDSLVRQEGAEAFRARISQQAQPLAEYFFRHLMEEADPATLEGKA
                     HLATLASPLLDKLPGANLRALMRQRLGEITGLSGESLGALAGSPTNDVPPADFAPSYD
                     PDSHAGYPAYPDERSDWQAGQGQGKRKWQDGGDWKGKKNGQRREPPAVMPPRGGAGVE
                     P"
     gene            complement(1704..1919)
                     /gene="rpsU"
                     /locus_tag="VIO83_10470"
     CDS             complement(1704..1919)
                     /gene="rpsU"
                     /locus_tag="VIO83_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY8331736.1"
                     /translation="MPAVKVKENEPFDVALRRFKRSCEKAGVLADVRSREFYEKPTAE
                     RKRKAAAAVKRHAKKVQREQRRRERLY"
     gene            2160..3200
                     /gene="tsaD"
                     /locus_tag="VIO83_10475"
     CDS             2160..3200
                     /gene="tsaD"
                     /locus_tag="VIO83_10475"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY8331737.1"
                     /translation="MRVLGLETSCDETGVALYDSERGLLADALFSQIDLHRAYGGVVP
                     ELASRDHVKRMLPLIREVLAESGCSFADIDAIAYTAGPGLVGALLVGASCAQALALAW
                     GVPAVGVHHMEGHLLAPMLEEAPPQFPFVALLVSGGHTQLVRVDGIGQYQLLGESLDD
                     AAGEAFDKTAKLIGLAYPGGPEIARLAERGTPGRFVFPRPMTDRPGLDFSFSGLKTFA
                     LNTWRQCEAEGGDLEEARCDVALAFQQAVVETLTIKCRRALKQTGLQRLVIAGGVSAN
                     QALRAALQTMLGELKGQVFYARPRFCTDNGAMIAYAGCQRLLAGQHDGPAIGVQPRWS
                     MEALPSLQAAQE"
     gene            complement(3208..>3406)
                     /locus_tag="VIO83_10480"
     CDS             complement(3208..>3406)
                     /locus_tag="VIO83_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY8331738.1"
                     /translation="AAWLLTFALTRISSLAALVATPLTLPLLAWQQPTALLPVTLLAA
                     LIVWRHRSNLRDLYAGRERHF"
BASE COUNT          500 a         1072 c         1243 g          591 t
ORIGIN      
        1 gcggttcgac gccggcaccg ccgcgcggcg gcattacggc cggcggctcg cggcgctgcc
       61 cgttcttctt gcctttccag tcgccgccgt cctgccactt gcgcttgcct tggccctggc
      121 cggcttgcca gtcgctgcgc tcgtccggat aggcgggata acctgcgtgg ctgtccggat
      181 cataagacgg tgcgaagtcc gctggcggca cgtcattggt cggcgagccg gccagtgcgc
      241 cgaggctctc gccgctgagg ccggtgatct cgccgaggcg ctggcgcatc agggcgcgca
      301 ggttggcgcc gggcagcttg tcgagtagcg gcgaggcgag ggtggcgagg tgcgccttgc
      361 cctcgagggt ggccgggtcg gcctcctcca tcagatggcg gaagaagtac tcggccagcg
      421 gctgggcctg ctggctgatg cgcgcccgga aggcttcggc gccttcctgg cgtaccagac
      481 tgtccggatc ctcgccttcg gggaggaaca ggaagcgcac ccggcggccg tcctgcaggc
      541 tcggcaggca ggcctcgagg gcgcgccagg cggccttgcg tccggcctgg tcgccgtcga
      601 agcagaacag gatgttcggc accaggcgga acaggcgctt gatgtgctcc tcgctggtgg
      661 cggtgccgag ggtggcgacg gcgttgcgca ggccctgctg ggcgagggcg atgacgtcca
      721 tgtagccctc gaccaccatg acctcgtcga gatcgcggtg gtgctggcgc gcctcgtaca
      781 ggccgtagag ctcctggccc ttgtggaaca ccggggtttc cggcgagttc aggtacttgg
      841 gcttgtcgtc gccgagcacc cggccgccga aggcgatcac ccggccgcgg ctgtcgcgga
      901 tcgggaacat cacccggtcg cggaagcggt cgtagcgctt gccggtctcc gggttgtcga
      961 tcagcaggcc ggcgtcgatc atggccttct gctgcagggt gtcgccgccc aggtgcttca
     1021 tcaggttgtc ccagccgggc ggggcgaagc cgagcccgaa gtcgcgggcg atcaccccgg
     1081 acaggccgcg gcccttgagg tagtccaccg cggcctggcg tgccggatgg ctcttcaggg
     1141 cctggcggta gaagccggcg gcggcgtcga gcagcgggta gagcggcgag tcgactgcct
     1201 ggcgcggctt ctgtccggga cggctgtcct cgtgcggcac ctcgaggccg gcgcgcttgg
     1261 ccagctcctc gaccgcctcg ggaaactcca ggtggtcgtg gtccatgatg aagccgaggg
     1321 cgttgccgcc ggcgccgcag ccgaagcagt agtagaactg cttgtccggg ctgacgctga
     1381 acgagggggt cttttcgttg tggaacgggc agcgggcgct gtagttcttg ccggtcttct
     1441 tcagctggat gcgcgaaccc accacctcga cgatgtcggt gcggttgagc aggtcgtcga
     1501 tgaagcgttg cgggatcagg ccggccatgg gctgctcgtg tgcatgctgt tgagcatggc
     1561 tgcccgctgc gccaaaggcc aggcaggggc gaatgcaaaa aactccgctc cttgcggggc
     1621 aaggcggagt gaaaagatga cgctaaagca aacacccggc cggagccggg tgttgggcga
     1681 aacgcaactg tccgagggtt aactcagtac aggcgctcgc gacgacgctg ctcgcgctgc
     1741 actttcttgg cgtggcgctt tacggcagcc gcggccttgc gcttgcgctc ggcggtgggc
     1801 ttctcgtaga actcgcggct acgcacatca gccagaacac cggctttttc gcaggagcgc
     1861 ttgaaacgac gcagggctac gtcgaagggt tcgttctctt taactttgac ggcgggcatc
     1921 caggtcgtac cttcacttaa taccgagtat gaatggtgca atcctgcaaa aggctgggga
     1981 ggcaccggtt ttcaagggct gcggatatta accctttgcc gggggaaatg ccaagcccct
     2041 gacggcaaaa cccgcctacc ggtagtccgc tgccggcgcc aaggcttggt gtccgtgccc
     2101 cggcccgctt atcatgcgcg gtttcccgaa cgatcacggc aatacgaggc gatctgccaa
     2161 tgcgagtgct gggcctggaa acttcctgcg acgaaaccgg cgtggcgctg tatgacagcg
     2221 agcgcggtct gctggccgat gcgctgttca gccagatcga cctgcatcgc gcctacggcg
     2281 gcgtggtgcc cgaactggcc tcgcgcgacc acgtcaaacg catgctgccg ctgatccgcg
     2341 aggtgctggc cgagtccggt tgcagtttcg ccgatatcga tgcgatcgcc tataccgccg
     2401 ggccgggact ggtcggcgcg ctgctggtcg gtgcctcctg cgcccaggcg ctggccctgg
     2461 cctggggcgt gccggcggtc ggcgtgcacc atatggaggg ccatctgctg gcgccgatgc
     2521 tggaggaggc gccgccgcag ttcccgttcg tcgccttgct ggtttccggc ggccacaccc
     2581 agctggtgcg ggtcgacggc attggtcagt accagctgct tggcgagtcg ctggacgatg
     2641 ccgccggcga agctttcgac aagaccgcca agctgatcgg tctcgcttat ccgggtggac
     2701 cggaaattgc tcgtctagcc gagcgtggca caccggggcg cttcgtcttc ccgcggccga
     2761 tgaccgatcg gccgggactg gacttcagtt tcagcggcct gaagaccttc gccctcaata
     2821 cctggcggca gtgcgaggcg gagggcggtg acctcgagga ggcccgctgc gacgtcgccc
     2881 tggccttcca gcaggcggtg gtggagacgc tgaccatcaa gtgccgccgg gcgctcaaac
     2941 agaccggcct gcagcgtctg gtgatcgccg gcggggtgag cgccaaccag gcgttgcgcg
     3001 cggcgctgca gacgatgctc ggcgagctca agggccaggt gttctatgcc cggccgcgct
     3061 tctgcaccga caatggtgcg atgatcgcct acgctggctg tcagcgtctg ctggccggcc
     3121 agcacgacgg cccggcgatc ggcgtgcagc cgcgctggtc gatggaagcg ctgccgtcgc
     3181 tgcaggcggc acaggagtag gccgggatta gaaatgccgt tcgcggccgg cgtagaggtc
     3241 gcgcaggttg ctgcggtggc gccagacgat cagagcggcg agcagggtga ccggcagcag
     3301 cgcggtcggc tgttgccagg ccagcagcgg cagggtcagc ggggtggcaa ccagcgcggc
     3361 gagcgagctg atgcgcgtca gggcgaaggt cagcagccag gcggcg
//
LOCUS       DATCUN010000269         2583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_219153, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000269 DATCUN010000000
VERSION     DATCUN010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219153"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..384
                     /locus_tag="VIO83_10485"
     CDS             25..384
                     /locus_tag="VIO83_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331739.1"
                     /translation="MSTIRETAESLGVDRIRRHIFLCADASEPKCCPREVGIESWNFL
                     KKRLNELKLTGDGGVYRTKAGCLRICEQGPVAVVYPDGTWYHSCTPPVLERIIQEHLI
                     GGRVVQDNLIIEHRLDD"
     gene            402..1055
                     /locus_tag="VIO83_10490"
     CDS             402..1055
                     /locus_tag="VIO83_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8331740.1"
                     /translation="MLQFEIIPVTPLQQNCTLLLCPATGNAALVDPGGDVEDIVAAIE
                     RHGAHLEKIFLTHGHLDHVGGTVTLRDRYRVPVEGPHPADAFWIESLPMQAQMFGFPP
                     LPAFTPDRWLEGGDTVRFGQIELAVRHCPGHTPGHVIFFDAQSRLALVGDVLFAGSIG
                     RTDLPRGNHADLLRSIPEQLWPLGKDVRFIPGHGPMSTFGAERRSNPYVADSVLARG"
     gene            complement(1449..1718)
                     /locus_tag="VIO83_10495"
     CDS             complement(1449..1718)
                     /locus_tag="VIO83_10495"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY8331741.1"
                     /translation="MATNSSSSEVQVGAQGRLVIPATLRKALHLEPGDRLVARQEGET
                     LVLERREAIQKRLQDRFRHIPSDVNLVNELIAERRAEAAKEAAQE"
     gene            complement(1890..2237)
                     /locus_tag="VIO83_10500"
     CDS             complement(1890..2237)
                     /locus_tag="VIO83_10500"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY8331742.1"
                     /translation="MAYVRACSVNDVPTGGMARFKVNGEAVVVYNLADGFHATAASCS
                     HVFGPLARGKILDGQRIQCPLHRAEFDIKTGEAVKWACFPPGVQLLNMFRPKKPIKTF
                     ATKVEGDAVFVDV"
     gene            complement(2290..>2583)
                     /locus_tag="VIO83_10505"
     CDS             complement(2290..>2583)
                     /locus_tag="VIO83_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8331743.1"
                     /translation="AYSATKAGVLGLTRSLAVDWAVDGIRVNAVAPGWVATDMTQGLR
                     DNRHLHDSLTARVPMGRFAQPNEIADVALFLASDSARYVTGSVYSVDGGLLAG"
BASE COUNT          427 a          899 c          836 g          421 t
ORIGIN      
        1 gcaccaaccc cttcgaggaa ttccatgagc accatccgcg agaccgccga atccctgggc
       61 gtggaccgaa tccgccgcca catcttcctg tgcgccgacg ccagcgagcc gaagtgctgc
      121 ccgcgcgagg tcggcatcga atcctggaat ttcctcaaaa aacgcctgaa cgagctcaag
      181 ctcaccggcg acggcggcgt ctaccgcacc aaggccggct gcctgcgcat ctgcgagcag
      241 gggccggtgg ccgtggtcta cccggatggc acctggtacc actcctgcac cccgccggtg
      301 ctggagcgga ttatccagga gcacctgatc ggcggccggg tggtgcagga caacctcatc
      361 atcgagcaca ggctggacga ctgacgccgc cgatgtcaga catgcttcag ttcgaaatca
      421 tcccggtcac gccgctgcag cagaactgca ccctgctgct gtgcccggcg accggcaatg
      481 cggcgttggt cgatccgggt ggcgacgtgg aggacatcgt cgcggcgatc gaacgccacg
      541 gcgcgcatct tgaaaaaatc ttcctcaccc acggccatct ggaccacgtc ggtggcacgg
      601 tgacgctgcg cgaccgctac cgcgtgccgg tcgagggccc gcacccggca gacgcgttct
      661 ggatcgagag cctgcccatg caggcgcaga tgttcggctt cccgccgctg ccggccttca
      721 cccccgaccg ctggctcgag ggcggcgaca cggtgcgctt cgggcagatt gaactggccg
      781 tgcgccactg ccccggccac acgccggggc atgtgatttt cttcgacgcg caaagccgcc
      841 tggcgctggt gggcgacgtg ctgttcgccg gctccatcgg ccgcaccgac ctgccgcgcg
      901 gcaatcacgc cgacctgctg cggtccatcc ccgaacagct gtggccgctg ggcaaagacg
      961 tgcgcttcat ccccggccac ggccccatgt ccactttcgg cgccgagcgg cgcagcaacc
     1021 cgtacgtggc cgactcggtg ctggcgcgcg gctgaggggc gcatgcctgg ccctcagcgg
     1081 atgagctgta tatgaaggcc caggcccagg ccggaccagg ctcggtccgc ggtaaggatc
     1141 ggcagctggt gttctatggc cagcgccagg caggctcgat cgcctaagga caggccctgc
     1201 tggcgagtct tgtcccacag gagggcggcg atctcggcct gctcgaccgt aaggtgagaa
     1261 aacgacgcca gcctgcacga attcctgccg catcccggtg atatcgacct ggcggcgcag
     1321 ggatttctgt acgacctccg accggttcac ggcggaaacc acggcctccg acaataccga
     1381 ccagacccgg tcggcccctg gctcatcgtg cagaaaggcc agcaaactcg acgggtcgag
     1441 ggccagcgtc actcctgcgc cgcctccttt gccgcctcgg cgcgccgctc cgcgatcagc
     1501 tcattcacca ggttgacgtc gctgggaatg tggcgaaacc gatcctggag ccgtttctgg
     1561 attgcctcgc gccgctcaag caccagcgtt tcgccttctt gccgcgccac cagccgatcc
     1621 ccgggttcaa gatgcagcgc cttgcgcagg gtggccggaa tcaccagccg tccctgtgca
     1681 ccgacctgga cttcgctgct cgaagaattt gttgccatgt cgtccactgc cgtgccaact
     1741 gaagttcagt gtggcacagg gcagcgcgtc gaccaaggca aagggctcgg cgatcgaccg
     1801 gaacctttgt ttgcgctgcc tggccatcag gctgcgcaaa ctcgacgaag cctgcccact
     1861 cccgtgcggg acgtgggcag gctgcaagct caaacgtcga cgaacaccgc gtcgccttcc
     1921 accttggtgg caaaggtctt gatcggcttc ttgggccgga acatgttcag cagctgcacg
     1981 cccggcggga agcaggccca cttcacggcc tcgccggtct tgatgtcgaa ctcggcccgg
     2041 tgcagcgggc actgaatgcg ctgaccgtcg agaatcttgc cgcgggccag cggaccgaac
     2101 acgtgggagc agctcgcggc ggtggcgtgg aagccgtccg cgaggttgta gacgacgacc
     2161 gcttcgccgt tgaccttgaa gcgggccatg ccgccggtcg gtacgtcgtt cactgaacag
     2221 gcgcgtacgt aagccattgc actctccctg gcagggggcg gcctgcgccg ccgtggtgtg
     2281 agacctcggt cagcccgcga gcaggccgcc gtccacactg taaaccgagc cggtcacgta
     2341 gcgcgcgctg tccgaggcca ggaacagcgc cacgtcggcg atttcgttcg gttgggcgaa
     2401 gcggcccatc ggcacgcgcg cggtcaggct gtcgtgcaga tgccggttgt cgcgcaggcc
     2461 ctgcgtcatg tcggtggcca cccagccggg cgccacggcg ttcacgcgga tgccatcgac
     2521 cgcccagtcg accgccaggc tgcgcgtcag gcccagcacg ccggccttgg tggcgctgta
     2581 ggc
//
LOCUS       DATCUN010000270         3196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_68335, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000270 DATCUN010000000
VERSION     DATCUN010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68335"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VIO83_10510"
     CDS             <1..191
                     /locus_tag="VIO83_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019822243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY8331744.1"
                     /translation="DELPAHLLRLFAAERATVEITLLEPIASAGLSRSELARRAHAAI
                     AGVVGAGEQNAASEAQAA"
     gene            complement(213..791)
                     /locus_tag="VIO83_10515"
     CDS             complement(213..791)
                     /locus_tag="VIO83_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HEY8331745.1"
                     /translation="MNYLAHLHLGGPQPGQLLGSLYGDFVKGPLAGRYPPDVEAAIRL
                     HRRIDVYTDSHPLVRTAVARFPAERRRYAGILLDLFFDHCLACGWADYAAEPLPQFTA
                     RVYQVLAEEAALPGRLALIAPRMAEQDWLGSYREFAVLERVLLNLQRRLSRPEGLDGA
                     LLELERLYPVLLEDFRAFYPQLMAFARAAQTD"
     gene            937..1239
                     /locus_tag="VIO83_10520"
     CDS             937..1239
                     /locus_tag="VIO83_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8331746.1"
                     /translation="MSFDTDEIIKALAHPVRREILHWLKAPEQYFADQEHSLEIGVCA
                     GMIDRRTGLSQSTVSAHLATLQRAGLVTSRKVGQWNFFKRNEDTIQAFLRRLDDEL"
     gene            1264..2328
                     /locus_tag="VIO83_10525"
     CDS             1264..2328
                     /locus_tag="VIO83_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY8331747.1"
                     /translation="MPTPSVPTLFDPLQVGDLQLPNRIIMAPLTRCRAEPGRVPGALM
                     AEYYVQRASAGLIISEATSVTPMGVGYPDTPGIWSAEQVQGWKNVTSAVHAAGGRIIL
                     QLWHVGRISDPSYLDGQLPVAPSAIQPKGHVSLIRPMKAFETPRALETEEIAGIVEAY
                     RQGAENAKAAGFDGVEIHGANGYLLDQFLQDSTNQRGDQYGGSLENRARLMLEVTDAV
                     ISVWGAGRVGMHLAPRADSHDMGDSNRAETFGYVARELGKRGIAFICAREKEGEDSLG
                     PQLKQIFGGVYIANERFTKEQSNRWLAEGKADAVAFGIPFIANPDLPARLAADAPLNE
                     PDSSTFYGSGPVGYLDYPTL"
     gene            complement(2398..>3196)
                     /locus_tag="VIO83_10530"
     CDS             complement(2398..>3196)
                     /locus_tag="VIO83_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEY8331748.1"
                     /translation="REPAFSADAPAPRSRAMPAPATVRAASTRPLWALVGVLLLALAG
                     LGWWSQQRLEQMAQQLVATQESFARVSEDAAGRLKDISGKVVATESSVTSESEALKLR
                     IKQLENKLAQLGKQQQTAAAQQETQGQQLAQLRGELKTQQAGTVQLAAALKEQQSGVA
                     SLDSRLQGLAGEQAALKTAQGALGKLESRLDSLGSEVAALKKQGNPAQAIDRLESDML
                     VLRSQLDNRPAAAAGSAELDAFRAQMTRNITTLQAQVQNLQQQLDAR"
BASE COUNT          536 a         1161 c         1034 g          465 t
ORIGIN      
        1 acgacgagct gccggcgcac ctgctgcgcc tgttcgccgc cgagcgcgcc acggtggaga
       61 tcaccctgct tgaaccgatc gccagcgccg gcctgtcgcg cagcgaactg gcgcgccgcg
      121 cccatgccgc gattgccggg gtagtcggcg ccggcgagca gaacgccgca agcgaagcgc
      181 aagcggcctg agccagggtc aggctgacga cgtcagtccg tctgcgccgc ccgggcgaag
      241 gccatcagct gcggatagaa cgcgcggaag tcctccagca gcacggggta caggcgctcc
      301 agctcgagca gcgcaccgtc caacccctcg ggccgcgaca ggcgccgctg caggttcagc
      361 agcacccgct cgagcacggc gaactcgcgg tagctgccca gccagtcctg ctcggccatg
      421 cgcggggcga tcagcgccag gcgcccgggc aacgccgcct cctcggccag cacctggtac
      481 acgcgcgcgg tgaactgcgg cagcggctcg gccgcgtagt cggcccagcc acaggccagg
      541 cagtggtcga agaacaggtc gagaaggatg ccggcgtagc gccgccgctc ggcgggaaag
      601 cgcgccacgg ccgtccgtac cagcgggtga ctgtcggtgt agacatcgat gcgccggtgc
      661 aggcggatgg ccgcctcgac gtccggcgga tagcgcccgg ccagcggccc cttgacgaag
      721 tcaccgtaca ggctgccgag cagttgcccc ggctgcggac cgccgaggtg caggtgggct
      781 agatagttca tgggcacagc ttaacgccgc ggccatcccc atcgaaagcc gcagaaggaa
      841 tgagccgccc atcgctgggg acgattcaat atttgtgaat cgcgaaacac cgatatatat
      901 ttcgccccat cgcgataaaa cattgcagcc acccccatgt cattcgatac cgacgaaatc
      961 atcaaggccc tggcccatcc cgtgcgccgc gagatcctcc actggctgaa ggccccggaa
     1021 cagtacttcg ccgatcagga gcactcgctg gagatcggcg tgtgcgccgg gatgatcgac
     1081 cgccgcaccg gcctgtcgca gtccaccgtg tcggcgcacc tggccaccct gcagcgtgcc
     1141 ggcctggtga ccagcaggaa ggtcggccag tggaacttct tcaagcgcaa cgaggacacc
     1201 atccaggcct tcctgcgccg gctcgatgac gaactctgac tccactcagc aaggaaaccg
     1261 accatgccga ccccttccgt tccgaccctg ttcgaccccc tccaggtagg cgatctgcaa
     1321 ctgcccaacc gcatcatcat ggccccgctg acccgctgcc gcgccgagcc cggccgcgtg
     1381 cccggcgcgc tgatggccga gtactacgtg cagcgcgcca gcgccggcct gatcatcagc
     1441 gaagccacct cggtgacgcc gatgggcgtc ggctaccccg acacccccgg catctggtcc
     1501 gcggaacagg tgcagggctg gaagaacgtg accagcgcgg tgcacgccgc cggcgggcgc
     1561 atcatcctgc agctgtggca cgtcggccgc atctccgacc cgagctacct ggacggccaa
     1621 ctgccggtgg cgcccagcgc catccaaccc aagggccacg tcagcctgat ccgccccatg
     1681 aaagccttcg aaaccccgcg tgcgctggaa accgaagaga tcgccggcat cgtcgaggcc
     1741 taccgccagg gcgcggagaa cgccaaggcc gccggcttcg acggcgtgga gatccacggc
     1801 gccaacggct acctgctcga ccagttcctg caggacagca ccaaccagcg cggagaccag
     1861 tacggcggct cgctggaaaa ccgcgcgcga ctgatgctgg aagtcaccga cgcggtgatt
     1921 tcggtgtggg gtgccggccg cgtcggcatg cacctggcgc cgcgcgccga cagccacgac
     1981 atgggcgact ccaaccgcgc cgagaccttc ggctacgtcg cccgcgagct gggcaagcgc
     2041 ggcatcgcct tcatctgcgc tcgcgagaag gaaggcgagg acagcctcgg cccgcagctc
     2101 aagcagatct tcggcggcgt gtacatcgcc aacgagcgct tcaccaagga gcagtccaac
     2161 cgctggctgg ccgaaggcaa ggccgacgcg gtggccttcg gcatcccgtt catcgccaac
     2221 cccgacctgc cggcacgcct ggctgccgac gcgccgttga acgagccgga cagcagcacc
     2281 ttctatggct ccggcccggt cggctacctc gactacccga ccctgtaaga ctcgcggcga
     2341 tgaaaaaaag ccccggttgc cattggcagc cggggctttt ctttgccacg ccggaactca
     2401 gcgcgcgtcg agctgctgct gcagattctg cacctgcgcc tgcagggtgg tgatgttgcg
     2461 ggtcatctgc gcgcggaagg catccagctc ggcactgccg gcggcggccg ccgggcggtt
     2521 gtccagctga ctgcgcagca ccagcatgtc ggattcgaga cggtcgatgg cctgcgccgg
     2581 attgccctgc ttcttcagcg ccgcgacctc gctgccaagg ctatccagcc gactttccag
     2641 cttgcccagc gcgccttgcg cggtcttcag cgcggcctgc tcgccggcca ggccctgcag
     2701 gcgactgtcc aggctggcaa cgccgctctg ctgctccttc agtgccgcag ccagctggac
     2761 cgtgccagcc tgctgggtct tcagttcgcc gcgcagctgc gcgagctgct ggccctgggt
     2821 ttcctgctgg gcggcagcgg tctgctgctg cttgccgagt tgggccagct tgttctccag
     2881 ctgcttgatg cgcagcttga gcgcctcgct ctcgctggtg acgctggact cggtggctac
     2941 caccttgccg gaaatgtcct tcagccggcc cgccgcatcc tcgctgacgc gcgcgaagct
     3001 ttcctgggtc gccaccaact gctgcgccat ctgttcaagg cgctgctggc tccaccaacc
     3061 caggccggcc agggccagca gcagcacgcc gaccagcgcc cacagcggcc gggtcgaagc
     3121 cgcgcgcacg gtggcgggag ccggcatggc gcgactgcgc ggcgccggcg catctgcaga
     3181 aaacgccggc tcgcgg
//
LOCUS       DATCUN010000271         7819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_143427, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000271 DATCUN010000000
VERSION     DATCUN010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143427"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..91
                     /locus_tag="VIO83_10535"
     CDS             <1..91
                     /locus_tag="VIO83_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331749.1"
                     /translation="GLLDADPQLLRPTARGQLFLNDLLQHFLP"
     gene            170..493
                     /locus_tag="VIO83_10540"
     CDS             170..493
                     /locus_tag="VIO83_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3392 domain-containing protein"
                     /protein_id="HEY8331750.1"
                     /translation="MDLLLDLFVTLSRWCRSHLDEIALAVVAALLVLFGPLVNAWLQR
                     QVGSLNFVFRTLLFILLCALGYGMVIVHATPWLEKGLAQLNNYTLAPALLLVFIGIGV
                     IAERR"
     gene            complement(802..1473)
                     /gene="trmB"
                     /locus_tag="VIO83_10545"
     CDS             complement(802..1473)
                     /gene="trmB"
                     /locus_tag="VIO83_10545"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEY8331751.1"
                     /translation="MRTIKSFVMRAGRVTEGQQRGLELGWPKFGLQLEDGLRDFDQLF
                     GRQAPRTFEIGFGMGHATLEMAAAAPEQDFIGVEVHKPGVGALLSGALAQNLTNIRVY
                     SCDALDVLRQCVADASLDRVLLFFPDPWHKAKHNKRRIVQPAFAELVRQKLKVGGVLH
                     MATDWQPYAEHMLEVLSAAPGYRNLAADGGYVPRPEERPVTKFERRGERLGHGVWDLK
                     FQRVD"
     gene            complement(1625..2422)
                     /locus_tag="VIO83_10550"
     CDS             complement(1625..2422)
                     /locus_tag="VIO83_10550"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY8331752.1"
                     /translation="MSPSSRTDKPLVLAGRTYHSRLLVGTGKYKDLDETRLAIEASGA
                     EIVTVAVRRTNIGQNPGEPNLLDVISPERYTILPNTAGCYDAVEAVRTCRLARELLDG
                     HKLVKLEVLADQKTLFPNVIETLKAAETLVKDGFDVMVYTSDDPVIARQLAEIGCIAV
                     MPLAGLIGTGLGICNPYNLRIILEEATVPVLVDAGVGTASDAAIAMELGCEAVLMNSA
                     IAHAQNPVLMAEAMKHAIVAGRLAYLAGRMPKKLYASASSPLEGLIR"
     gene            complement(2498..2698)
                     /gene="thiS"
                     /locus_tag="VIO83_10555"
     CDS             complement(2498..2698)
                     /gene="thiS"
                     /locus_tag="VIO83_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY8331753.1"
                     /translation="MRIQLNGEAVELPEGHSVADLLAGLDLAGRRVAVELNLDIVPRS
                     QHAVTLLREGDRVEVVHAIGGG"
     gene            complement(2793..3176)
                     /locus_tag="VIO83_10560"
     CDS             complement(2793..3176)
                     /locus_tag="VIO83_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEY8331754.1"
                     /translation="MSRPFVLLAAFFGFTGVALGAFAAHGLRGRLSPEYLAVFHTGVL
                     YQLIHALALLGIGALALHWRSRLLSAAGSLFAAGILLFSGSLYLLTLSGVGKLGMVTP
                     LGGTCLLAGWLCVALAAWRAQSRRH"
     gene            3351..4043
                     /gene="mtgA"
                     /locus_tag="VIO83_10565"
     CDS             3351..4043
                     /gene="mtgA"
                     /locus_tag="VIO83_10565"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEY8331755.1"
                     /translation="MPRRLFRRLAKILLCCLAASVLLVLLLRWLPPPGSALMVERKIE
                     SWLDGQPIDLQRDWRPWAELPDDLKVAVIAAEDQRFAEHWGFDMAAIRAAMAHNERGG
                     SLRGASTLSQQVAKNLFLWSGRSWLRKGLEAWFTVLIETLWPKQRILEVYLNSAEWGK
                     GVFGAEAAARHHFGKGAPYLSSQQASLLAAVLPNPRDWSASRPSGYVQQRAGWIRRQM
                     RQLGGSYYLNRL"
     gene            complement(4156..5010)
                     /gene="rpoH"
                     /locus_tag="VIO83_10570"
     CDS             complement(4156..5010)
                     /gene="rpoH"
                     /locus_tag="VIO83_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEY8331756.1"
                     /translation="MSTSLQPVYALAPGANLEAYVHSVNSIPLLSVEQERELAGRLYY
                     AQDLEAARQMVLAHLRFVVHIARSYSGYGLAQADLIQEGNVGLMKAVKRFNPEMGVRL
                     VSFAVHWIRAEIHEFILRNWRIVKVATTKAQRKLFFNLRSQKKRLAWLNNDEVNAVAA
                     TLGVEAREVREMESRLTGHDMAFDPAADADDDAAFQSPAHYLEDNRYDPARQLEEADW
                     SDSSNASLHDALEGLDERSRDILQQRWLSEEKATLHELAAKYNVSAERIRQLEKNAMN
                     KLKGHLLA"
     gene            complement(5133..6146)
                     /gene="ftsX"
                     /locus_tag="VIO83_10575"
     CDS             complement(5133..6146)
                     /gene="ftsX"
                     /locus_tag="VIO83_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY8331757.1"
                     /translation="MSATKQLPRGSEEGSPEQRQRRLQQEAEATDWGGLLRAYLESHR
                     ASLVDSLRRLVHQPFGSFLTCLVMGIALSLPMGLSLLLDNVERLGGSWQRAAQISVFL
                     KLEVAEVEGQALREQIAGMDDVLQADWIGREEALAQLQEHSGLGDALKDLPENPLPAV
                     VLVTPQEIDKAALEALRERLAALPGVEQAQLDLLWVERLGAILQLGDRFVFGLAVVLV
                     LALLLVIGNTIRLHIENRRAEIEVIKLVGGTDGYVRRPFLYAGAFYGVGAGVLAWGLL
                     EYGLGWLNQSVVSLAGLYGSDFALAGVPAEDGLSLLVGAVLLGWVGAWLAVARHLRQL
                     EPQ"
     gene            complement(6146..6814)
                     /gene="ftsE"
                     /locus_tag="VIO83_10580"
     CDS             complement(6146..6814)
                     /gene="ftsE"
                     /locus_tag="VIO83_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEY8331758.1"
                     /translation="MIRFEQVGKRYPNGHVGLHELSFRARRGEFLFVTGHSGAGKSTL
                     LRLLLALERPTSGKLLLAGQDLGEISNAQIPFVRRQIGVVFQNHHLLFDRSVFANVAL
                     PLQILGLPREEIRQRVDAALERVSLKDMGELLPADLSSGQQQRVGIARAVVHQPALLL
                     ADEPTGNLDPRLAAEIMGVFEDINRLGTTVLIASHDLALIARMRHRLLTLQRGRLIGD
                     GEPD"
     gene            complement(6811..>7819)
                     /gene="ftsY"
                     /locus_tag="VIO83_10585"
     CDS             complement(6811..>7819)
                     /gene="ftsY"
                     /locus_tag="VIO83_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY8331759.1"
                     /translation="EVEPPLEAQPEPQPAAAAPASEAPAKLGFFARLRQGLSKTSASI
                     GEGMASLFLGKKAIDDDLLDELETRLLTADVGVEATTAIMQNLTKRVSRKELADSDAL
                     YKALQEELAAILRPVEQPLVIAAGHKPYVILVVGVNGAGKTTTIGKLAKKLQDEGKKV
                     MLAAGDTFRAAAVEQLQVWGERNKIPVIAQHTGADSASVIFDAVQAAQARGIDVLIAD
                     TAGRLHTKDNLMEELKKVRRVIGKLDDSAPHETLLVLDAGTGQNALSQARLFHQAVNL
                     SGLVLTKLDGTAKGGVIFALAKQLPTPIRYIGVGEGIDDLRPFEADAFVRALFAGQES
                     A"
BASE COUNT         1204 a         2671 c         2702 g         1242 t
ORIGIN      
        1 cggcctgctc gacgccgatc cgcagctgct gcggccgacc gcgcgcggcc agctgttcct
       61 caacgacctg ctgcagcact ttctgcccta agcgtgtgcg gcgtggcagg ccggtcactg
      121 ttctgcaggt aaactgcgcc gagcctttcc gtactagtca ggagtccgca tggatctgct
      181 actcgacctg ttcgtcaccc tgtcgcgctg gtgccgcagc catctcgacg agatcgccct
      241 ggcggtggtc gccgcgctgc tggtgctgtt cggcccgctg gtcaatgcct ggctgcagcg
      301 ccaggtcggc agcctgaatt tcgtgttccg caccctgctg ttcatcctgc tctgcgcgct
      361 gggctacggc atggtcatcg tgcatgccac cccctggctg gagaagggcc tggcgcagct
      421 gaacaactac accctggcgc cggcgctgct gctggtgttc atcgggatcg gcgtgatcgc
      481 cgagcggcgc tgatccgtac tggccttgat tcgtagggtg gacaacggct cagcctggtc
      541 ccccaatccg cgtgcaccgg cgctgcaaac ggcggagaaa ccggcgggcg gcgggtagcg
      601 gtagaaaatc gctacgcgag ttttctaccc tgcggtctgg actcgccgta acgccgcagg
      661 ctggcaaact cgcgccagcg attgtccacc gacctctgcg cgccaacgct ggaaaagacc
      721 ttcggtcgtt ttccacccta cgcaccgacc agacacgacg aagccgggca agcccggctt
      781 cgttcgcagc agcgtgagac gtcagtcgac gcgctggaac ttcaggtccc acacgccatg
      841 gccgaggcgc tcgccgcggc gttcgaactt ggtcaccggg cgctcctcgg gccgcggcac
      901 gtagccgccg tccgcggcca ggttgcggta gcccggcgcc gcgctcagca cctccagcat
      961 gtgctcggcg tagggctgcc agtcggtggc catgtgcagc acgccgccga ccttgagctt
     1021 ctggcgcacc agctcggcga acgccggctg cacgatgcgc cgcttgttgt gcttggcctt
     1081 gtgccaggga tcggggaaga acaacagcac gcgatccagg ctggcgtcgg ccacgcactg
     1141 gcgcagcacg tccagcgcgt cgcagctgta cacgcggatg ttggtcaggt tctgcgccag
     1201 ggcgccactg agcagggcgc cgacgcccgg cttgtgcacc tcgacgccga tgaagtcctg
     1261 ttccggcgcg gcggcggcca tctccagggt cgcgtggccc atgccgaagc cgatctcgaa
     1321 ggtgcgcggc gcttggcggc cgaacagctg gtcgaagtca cgcaggccgt cctccagctg
     1381 caggccgaac ttcggccagc ccagttcgag gccgcgctgc tggccctcgg tcacccgccc
     1441 ggcacgcatc acgaagctct tgatggtgcg caggcggcgc gcctcgccgg cagcttgcgg
     1501 cgcttgttgg atttcgtcag tcatgaacac tttcgctgta aagacaaacg agccctgccc
     1561 tcgcggaccg gactcgctgt gctgaagtcg gggcagcgcc agggcctgcc catggaaaag
     1621 ccgcttagcg gatcagccct tcgagtggcg aggaggcgct ggcgtagagc ttcttgggca
     1681 tgcgcccggc caggtaggcc aggcgcccgg cgacgatggc gtgcttcatc gcctcggcca
     1741 tcagcaccgg gttctgggcg tgggcgatgg cgctgttcat cagcaccgcc tcgcagccca
     1801 gctccatggc gatggcggca tccgacgcgg tccccacgcc ggcatccacc agcaccggca
     1861 cggtggcttc ctcgaggatg atgcgcaggt tgtacgggtt gcagatgccc aggccagtgc
     1921 cgatcaggcc ggccagcggc atcaccgcga tgcagccgat ctcggccagc tggcgggcga
     1981 tgaccgggtc gtcgctggtg tagaccatca cgtcgaagcc gtccttgacc agggtctcgg
     2041 cggccttgag ggtctcgatc acgttaggga acagggtctt ctggtcggcc agcacttcca
     2101 gcttgaccag cttgtggccg tcgagcagct cgcgggccag gcggcaggtg cgcactgctt
     2161 ccaccgcgtc gtagcagccg gcggtgttgg gcaggatggt gtagcgctcg gggctgatca
     2221 cgtcgagcag gttcggctcg ccggggttct ggccgatgtt ggtgcggcgc acggcgacgg
     2281 tgacgatctc cgcgccggag gcctcgatgg ccaggcgggt ctcgtcgaga tccttgtact
     2341 tgccggtgcc gaccagcagc cgcgaatggt aggtgcggcc ggccaggacc agcggtttgt
     2401 cggtacggga agacgggctc atcggggatt cctctgcagg cctgctcggg cggatcggga
     2461 aaaggacggc ggggcggtcg acggcgctga tggctgtcta gccgccgcca atggcatgca
     2521 cgacctcgac ccggtcaccc tcgcgcagca gggtgacggc gtgctggctg cgcggcacga
     2581 tatccaggtt gagttcgacc gccacgcgcc ggccggccag atcgaggccg gccagcaggt
     2641 cggcgacgga atgcccttcg ggcagttcca cagcttcgcc attgagctga atgcgcatgc
     2701 ttggccactc gcaacatcag ggggaaaggc cggcattcta gcgccgatcg gcgccgatga
     2761 ccaaggcgcc ggcggaccgc cgggctgccg actcagtggc ggcgtgactg ggcgcgccag
     2821 gccgccagcg ccacgcacag ccagccggcg agcaggcagg tgccgccgag cggggtgacc
     2881 atgcccagct tgccgacgcc gctgagggtc agcaggtaca ggctgccgga gaacagcagg
     2941 ataccggcgg cgaacaggct gccggccgcg ctgagcaggc gcgagcgcca gtgcagcgcc
     3001 agcgcgccga tgccgagcag cgccagggcg tggatcagct ggtagagcac gccggtgtgg
     3061 aacaccgcca ggtactcggg gctcagccgg ccgcgcaggc cgtgggcggc gaaggcgccg
     3121 agggccacgc cggtgaagcc gaagaaggcg gcgagcagga cgaagggacg cgacatggac
     3181 ggactccgca ggcgggaaca ggaactgcgc atgatcaccg cttggcggcc gccaggcaaa
     3241 ccgccgccgg cggcgtgcac ggccagcggc cgacgatagc gacgcgagtc accgccaggc
     3301 cgcgccgccc cgctataatg cccgcccctc ccgccgaacc cttcgccgcc atgccgcgcc
     3361 gcctgtttcg ccgcctcgcc aagatcctcc tctgctgcct ggccgccagc gtcctgctgg
     3421 tgctgctgct gcgctggctg ccgccacccg gcagcgcgct gatggtcgag cgcaagatcg
     3481 aatcctggct cgacggccag ccgatcgacc tgcaacgcga ctggcgcccg tgggcggaac
     3541 tgccggacga cctcaaggtg gcggtgattg ccgccgaaga ccagcgcttc gccgagcact
     3601 ggggtttcga catggccgcg atccgcgccg ccatggcgca caacgaacgc ggcggcagcc
     3661 tgcgcggcgc cagcaccctc agccagcagg tcgccaagaa cctgttcctc tggtccgggc
     3721 gcagctggct gcgcaagggc ctggaagcct ggttcaccgt gctgatcgag acgctgtggc
     3781 cgaagcagcg catcctcgag gtgtacctga acagcgccga atggggcaag ggcgtgttcg
     3841 gcgccgaagc ggcggcccgc catcacttcg gcaagggggc gccctacctg tcgagccagc
     3901 aggccagcct gctcgccgcc gtgctgccca acccgcgcga ctggagcgcc agccggccga
     3961 gcggctacgt gcagcagcgc gccggctgga tccgccggca gatgcgccag ctgggcggca
     4021 gctactacct caatcgcctg taggccccgg gtggcacgcc cccaatgcgc gggccggcaa
     4081 gcgtgaccga tcaccgtgca caaaaaagcc gcatccgggg atgcggcttt ttgcatcgtt
     4141 cagcgcgcct gccgatcagg ccagcagatg gcccttgagc ttgttcatcg cgttcttttc
     4201 cagctggcgg atacgctcgg cggagacgtt gtacttggcg gccagctcgt gcagggtggc
     4261 cttttcctcg gacagccagc gctgctggag gatgtcgcgg ctgcgctcgt cgaggccctc
     4321 cagggcatcg tgcaggctgg cgttggagct gtcgctccag tcggcctcct ccagctggcg
     4381 cgccgggtcg tagcgattgt cttccaggta gtgcgccggc gactggaagg ccgcatcgtc
     4441 gtcggcatcg gccgccggat cgaaggccat gtcgtggccg gtcaggcggc tttccatctc
     4501 gcgcacttcg cgcgcctcga cccccagggt cgcggccacg gcgttgactt cgtcgttgtt
     4561 cagccaggcc aggcgcttct tctggctgcg caggttgaag aacagcttgc gctgcgcctt
     4621 ggtggtggcg accttgacga tgcgccagtt gcgcaggatg aattcgtgga tctcggcgcg
     4681 gatccagtgc acggcgaagg acaccagacg cacacccatt tccgggttga agcgcttgac
     4741 cgccttcatc aggccgacgt tgccttcctg gatcaggtcg gcctgggcca ggccatagcc
     4801 ggagtagctg cgggcgatgt gcacgacgaa gcgcaggtgg gccagcacca tctggcgggc
     4861 ggcctcgagg tcctgcgcgt agtacagacg cccggccagc tcgcgctcct gctcgaccga
     4921 cagcagcgga atgctgttga ccgaatgcac gtaggcctcc aggttggcgc cgggagccag
     4981 ggcatagaca ggttgcagag acgtggacat ggacatcctc cgagttaccg aatcagctca
     5041 tgactatagc actgtgtcga taagactgtg aatggccgtg aaaagttctc ttacacctga
     5101 aaaataccaa taaaatcagc agcttatcat tatcactgag gctcgagctg acgcagatgc
     5161 cgggcaaccg ccagccaggc gccgacccag cccagcagca ccgcgccgac cagcagcgac
     5221 aggccgtctt ccgccggcac cccggccagg gcgaagtcgc tgccatacag gccggccagg
     5281 ctcaccaccg actggttcag ccagcccagg ccgtactcca gcaaccccca ggccagcacg
     5341 ccggcgccca cgccgtagaa ggcaccggca tacaggaacg gccggcgcac gtagccgtcg
     5401 gtgccgccga ccagcttgat cacctcgatc tcggcgcggc ggttctcgat gtgcaggcgg
     5461 atggtgttgc cgatcaccag cagcagggcg agcaccagca ccaccgccag gccgaagacg
     5521 aagcgatcgc caagctggag gatggcgccg aggcgctcga cccacagcag gtcgagctgc
     5581 gcctgctcga ccccgggcag cgccgccagg cgctcgcgca gggcctccag ggcggccttg
     5641 tcgatctcct gcggggtgac cagcaccacc gccggcagcg ggttttccgg cagatccttg
     5701 agggcgtcgc cgagaccgga gtgctcctgc agctgcgcca gcgcctcctc gcggccgatc
     5761 cagtcggcct gcagcacgtc gtccatgccg gcgatctgtt cgcgcagggc ctggccctcg
     5821 acctctgcca cctcgagctt gaggaacacc gagatctgcg cggcgcgctg ccaggagccg
     5881 ccgaggcgct cgacgttgtc cagcagcagc gacaggccca tgggcagcga cagggcgatg
     5941 cccatcacca ggcaggtgag gaagctgccg aacggctggt gcaccagccg gcgcaggctg
     6001 tcgaccaggc tggcgcggtg gctttccagg taggcgcgca gcaggccgcc ccagtcggtg
     6061 gcctcggcct cctgctgcag gcgccgctgg cgctgctccg gagagccttc ctcgctgccg
     6121 cgcggcagtt gcttggtggc actcatcagt cgggctcccc gtcgccgatc aggcgaccgc
     6181 gttgcagggt cagcaggcga tggcgcatgc gcgcgatcag ggcgaggtcg tggctggcga
     6241 tcagcacggt ggtccccagg cggttgatgt cctcgaacac ccccatgatc tcggcggcca
     6301 gacgcgggtc gaggttgccg gtcggctcgt cggccagcag caacgccggc tggtgcacca
     6361 ccgcgcgggc gatgccgacg cgctgctgct ggccgctgga caggtcggcc ggcagcagtt
     6421 cgcccatgtc cttgagcgac acccgctcca gcgcggcgtc cacccgctgg cggatctcct
     6481 cgcgcggcag gccgaggatc tgcagcggca gggcgacgtt ggcgaacacg ctgcggtcga
     6541 acagcaggtg gtggttctgg aacaccacgc cgatctgccg gcgcacgaag gggatctgcg
     6601 cgttgctgat ctcgcccaga tcctggccgg ccagcagcag cttgccgctg gtcggtcgtt
     6661 ccagcgccag cagcaggcgc agcagggtgc tcttgcccgc accggagtgg ccggtgacga
     6721 acaggaactc gccgcgccgg gcccggaaac tcagctcgtg caggccgacg tggccgttgg
     6781 gataacgctt gcccacctgc tcgaaacgaa tcatgcggac tcctgtcctg cgaaaagtgc
     6841 gcgtacgaag gcgtcggcct cgaacggacg cagatcgtcg atgccctcgc caacgccgat
     6901 gtagcgaatc ggcgtcggca gctgcttggc cagggcgaag atcaccccgc ccttggcggt
     6961 gccgtcgagc ttggtcagca ccaggccgct gaggttgacc gcctggtgga acagccgcgc
     7021 ctggctgagg gcgttctgcc cggtgccggc gtccagcacc agcagggtct cgtgcggcgc
     7081 gctgtcgtcg agcttgccga tcacccgacg gaccttcttc agctcttcca tcaggttgtc
     7141 cttggtgtgc aggcggccgg cggtgtcggc gatcagcacg tcgatgccgc gcgcctgggc
     7201 ggcctgcacc gcatcgaaga tcaccgacgc ggagtcggcg ccggtgtgct gggcgatcac
     7261 cggaatcttg ttgcgctcgc cccacacctg cagctgctcg accgcggcgg cgcggaaggt
     7321 gtcgccggcc gcgagcatga ccttcttgcc ctcgtcctgc agcttcttcg ccagcttgcc
     7381 gatggtggtg gtcttgccgg cgccgttcac gccgaccacc aggatcacgt agggcttgtg
     7441 gccggccgcg atcaccagcg gctgctcgac cggacgcagg atggcggcca gctcttcctg
     7501 cagcgccttg tacagcgcgt cgctgtcggc cagctccttg cgcgacacgc gcttggtcag
     7561 gttctgcatg atcgcggtgg ttgcctcgac gccgacgtcg gcggtgagca ggcgggtctc
     7621 cagctcgtcg agcagatcgt cgtcgatggc cttcttgccg aggaacaggc tggccatgcc
     7681 ctcgccgatg ctggcgctgg tcttcgacag gccctggcgc aggcgggcga agaagcccag
     7741 cttggccggg gcctcggacg ccggcgcggc agcggccggc tgcggttcgg gctgcgcttc
     7801 gagcggaggc tcgacttct
//
LOCUS       DATCUN010000272        10535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_182632, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000272 DATCUN010000000
VERSION     DATCUN010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10535
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182632"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1145
                     /gene="glk"
                     /locus_tag="VIO83_10590"
     CDS             186..1145
                     /gene="glk"
                     /locus_tag="VIO83_10590"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEY8331760.1"
                     /translation="MIILAADIGGTQARLLLGECEGDGWRTLRRQTLSSRDYPDFDTL
                     LQTFLLPGERPLTACVALAGPIDNQRVRMTNLPWQLDAAELAGRFGLRQVQLLNDFSA
                     QANGLALLRREDLCTLQAGEPPADGVRALLGAGTGLGMALLAGSPQQPLVLSSEGGHA
                     DFAPQDEQQLALWQHLRAEHGRVSLELLLSGHGLERLYRFIAGRAASEAVSLDARGIS
                     RMAAAGDAQACATLELFARLYASAAGNLALTGLARGGVYLCGGIAPKILPFLQQPQVR
                     EAFCSKPPMHELLQRIPLHVVLDEYLGLRGAAQVAARLASPAE"
     gene            1150..2928
                     /locus_tag="VIO83_10595"
     CDS             1150..2928
                     /locus_tag="VIO83_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY8331761.1"
                     /translation="MQPSSPPPAKTASPLSWQFMRLTSSYWNSERKWTIRGAVLLLIL
                     LTEAQVGLAIWISYWNRELFDALEARSLHDLLMLGLTFMLIFVLTMLVTAVHLHVKRW
                     LQLDWRRWLTTRLLDYWMAHARHYQLQFTSGEHDNPDGRIAEDIRIATESAIGLVLSL
                     LYSLLILGSFIEILLSVSGSLPIPGTSLEVPGYLVLLAFLYAGGGTVLGLLLGRPLIK
                     ATNRLQTVEANLRFGLARARENSESIALMHGEGIEHRQADKLFADVSLGWNRQTFGYL
                     GIVSFSTGYGTLLPVFPILIAAPQFIAGAMSLGILMQAAQAFQKLTSALSWPIDNLGE
                     LAKCRASADRVISLYNDMLLLEKRASNPVEHRIDLHTNRSDELALRGLSLANPDGKVL
                     LENLDLTIQRGERVLISGDPTLTIGLFKAVAGLWPWGHGEILLPAGQSIVFLPQRPFL
                     PDGTLQAALSYPHASGHFNNARIQHALTCAGVAWLAPRLEESDDWDRVLPLRAQQRLG
                     IARLLLHHPAWVFIEEATDAFDVQGEESMTEVLHRELQGSTLLTISFHSTLAHFYTRK
                     LVLDRICEDKYLTCSPEPAPCALREP"
     gene            complement(2914..4005)
                     /locus_tag="VIO83_10600"
     CDS             complement(2914..4005)
                     /locus_tag="VIO83_10600"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HEY8331762.1"
                     /translation="MKLRGVNLGSWLVLEKWMVPSLFEGMAATDETTWCAELGEAAGD
                     KLRRHWNSFITREDFAWLAEHGLNAVRIPLGHWIFGADYPYHPSYGEQRHPFVTGGIE
                     VLDRAMDWAQEFGLRVVLDLHAAPGCQNGFDNGGIKDVCEWHTKPEYLEHSLGVLERL
                     AERYRAHPALHAIEVLNEPRWDVPTDYLKAYNLAAYERIRKHCPAERVAVVFHDGFRS
                     FREYLGFMQAPQYRNVIFDIHRYQCFERGDIDMDIYGHIRKAAGDWKQEADAIITELG
                     LPTFCGEWSLGLDLKVVSLWAEGPFNHALEQMDAFQESVAYRGYAAGQLATYEKYLGW
                     FFWSYKTETTPAWCFRECVERGWLPSRFA"
     gene            complement(4130..4924)
                     /locus_tag="VIO83_10605"
     CDS             complement(4130..4924)
                     /locus_tag="VIO83_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8331763.1"
                     /translation="MFIDLSDKTAIVTGATGGIGLAIAKGLARAGATVVITGRDQERL
                     DAALARLRDAVPARKFRGVVADVATAAGCKQLIAAQPQADILINNLGIYAVCDFFEID
                     DDQWEEMFQVNVLSGIRLSRHYAQGMRERGWGRIQFISSESALNIPAEMVHYGVSKAA
                     LQGVSRGLAKVLAGTGVTVNSILPGPTRTEGVVNFLASMAEARGVLTTEMEALFIKEN
                     RPSSLLQRFADPDEIANMCVYVASPQASATTGAALRVEGGIVESIA"
     gene            5359..5985
                     /gene="ycaC"
                     /locus_tag="VIO83_10610"
     CDS             5359..5985
                     /gene="ycaC"
                     /locus_tag="VIO83_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008032356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HEY8331764.1"
                     /translation="MATPYKRLDKNQAAVLLVDHQAGLLSLVRDNDPDRFKNNVLALG
                     DLAKYFNLPTILTTSFESGPNGPLVPELREQFPAAPYIARPGQINAWDNEDFVAAVKA
                     TGRKQLIIAGVVTEVCVAFPALSAIAAGYEVFVVTDASGTFNELTRHSAWDRMSSAGA
                     QLMTWFGVACELHRDWRNDVEGLAALFSNHIPDYRNLMTSYATLAAAK"
     gene            6275..7573
                     /gene="dadA"
                     /locus_tag="VIO83_10615"
     CDS             6275..7573
                     /gene="dadA"
                     /locus_tag="VIO83_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEY8331765.1"
                     /translation="MRVLVLGSGVVGTASAYYLARTGFEVVVVDRQDGAALETSYANA
                     GQVSPGYAAPWAAPGIPLKALKWLLQRHAPLAIRLTADPAQYLWMAQMLRNCTATRYA
                     LNKERMVRLAEYSRDCLDELRAETGLTYEGRQLGTTQLFRSQAQVDAAARDITVLERA
                     GVPYEVLDRAGIVRVEPALAAVADKLAGALRLPNDQTGDCFLFTNHLAEMARTLGVEF
                     RFGQRIERLECDGARIRGVWIDGQLETADHYVLALGSYSPQLLKPLGIQLPVYPLKGY
                     SLTVPITNAAMAPTSTILDETYKVALTRFDKRIRVGGMAEIAGFDLALDPRRRATLEM
                     ITTDLFPQGGDLARADFWTGLRPATPDGTPIVGATRYRNLFLNTGHGTLGWTMACGSG
                     RLLADLMAARRPRIDPQCLDIGRYGREQRTLQPSHPTPAH"
     gene            complement(7599..8969)
                     /gene="nhaA"
                     /locus_tag="VIO83_10620"
     CDS             complement(7599..8969)
                     /gene="nhaA"
                     /locus_tag="VIO83_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015271374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEY8331766.1"
                     /translation="MDRHSSRQAIPRAQLLAERAFSTLERFLHIEAVSGIVLLVAAAA
                     ALIWANSPAADSYHALWHAPLSFGLGSYVVSQSLHFWINDALMTVFFLVVGMEIRREI
                     HEGALSSWRLASLPIVAALGGVMVPALIYLALNGEPVPRQGWAVPTATDIAFAVGVLA
                     LLGRSIPGNVRVLLLALAIIDDIIAVLIIAVFYSGGLDQTGLLVAGIGILMVLALQWI
                     GIGSAYAYIIPGAVLWLGFLKTGAHPTLAGVVLGLMTPVLPGRSQLRPLDAMTRAVSD
                     LCERATTPHPQEGQFVQSLKELRKAQRDLLPPVTRVQMALHPWVAYGVMPLFALANAG
                     VGLDGIDLQADGSLGVILGVLLALVVGKPLGVIVASWCVVRLGWCRLPPEVTWSGVCL
                     VGLLAGIGFTMSIFIATLAFSSETLLSAAKLGVLMASAIAAILGLTWGLVYTRRLRSA
                     SVPNTV"
     gene            9397..9648
                     /locus_tag="VIO83_10625"
     CDS             9397..9648
                     /locus_tag="VIO83_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331767.1"
                     /translation="MFFSTLGTALSLRKATPYDLNDFLFSPSELQHKYETRVRHSEHP
                     HFDRALWLRAVNAGACELGYWQWVFEQVQADDDTYLLRH"
     gene            complement(9902..>10535)
                     /locus_tag="VIO83_10630"
     CDS             complement(9902..>10535)
                     /locus_tag="VIO83_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004419243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8331768.1"
                     /translation="VLQDSTFELFDAGKLDFASGSAIVLSETRGAQVFGDFARYKERL
                     VLRPQEISNHPEVARRLGIIGINTALEFDIYGNVNSTHVGGTHMMNGIGGSGDFARNA
                     QLAIFVTKSIAKDGAISSVVPMVSHVDHTEHDVDILVTEQGLADLRGLAPRERARVII
                     DNCVHPDYREALGGYFAAACERGGQTPHLLEEALSWHINLGQRGHMLAAD"
BASE COUNT         1936 a         3643 c         3222 g         1734 t
ORIGIN      
        1 tgccttgagg atgcccgcca ccctcctata ctcaagaaaa aaatgagcag ccataaagag
       61 atacgagctg ccgacctttt gaaccatctt cgatagaatc ccgccccgcg ccgccgggta
      121 gagaaattct cgctactggc ggtcgcggca ggattcttcg catagacgcg tgtagagagg
      181 tggtcatgat cattctggcg gcggacattg gcggaaccca ggcgcgcctg ctgctaggcg
      241 agtgcgaggg ggacggttgg cggacgctgc gtcgccagac cctgtccagc cgtgactatc
      301 ccgacttcga caccctgcta cagaccttcc tgctgcctgg cgaacgccca ctgacggcgt
      361 gcgtcgccct ggccgggcca atcgataacc agcgggtacg gatgaccaac ctgccctggc
      421 aactggatgc tgccgaactg gccgggcgtt tcggtctgcg tcaggtgcag ctgctcaacg
      481 acttttccgc ccaggccaat ggcctggcgc tgctgcgccg cgaagacctg tgcaccctgc
      541 aggccggcga accaccggcc gatggcgtgc gcgccctgct cggcgccggc accggcctcg
      601 gcatggcctt gctggccggc agtccgcagc aaccgctggt gctgtccagc gagggcggcc
      661 acgctgactt cgctccgcaa gacgagcaac aactggcgct gtggcaacac ctgcgcgcgg
      721 agcatggccg tgtcagcctc gagctgctgc tgtccggcca cggcctggag cggctgtacc
      781 gcttcattgc cggccgagca gccagcgagg ctgtgtcgct cgacgcccgt ggaatcagcc
      841 ggatggccgc ggccggcgac gcgcaggcct gtgcgactct cgagctgttc gcccgcctgt
      901 acgcctcggc cgccggcaac ctggcgctaa ccggcctggc ccggggcggc gtgtacctgt
      961 gcggtggcat cgccccgaag atcctgccct tcctgcaaca gccacaggtg cgcgaagcct
     1021 tctgcagcaa gccgccgatg cacgagctgc tgcagcgcat tcccctgcat gtcgtgctcg
     1081 acgaatacct cggcctgcgc ggtgccgccc aggtcgccgc acgtctggcc agcccagcgg
     1141 agtaatcgca tgcaaccatc cagccctcct cccgcgaaaa ccgcctcgcc cctctcctgg
     1201 cagttcatgc gcctgaccag ctcctactgg aacagcgagc gcaagtggac catccgcggc
     1261 gcggtactgc tgctgatcct gctgaccgaa gcccaggtgg gactggcgat ctggatcagc
     1321 tactggaacc gcgagctgtt cgacgccctc gaggcgcgct cactgcatga cctgctgatg
     1381 ctggggctca ccttcatgct gatcttcgtg ctgaccatgc tggtgaccgc cgtgcatctg
     1441 cacgtcaagc gctggctgca actggactgg cggcgctggc tgaccacccg cctgctcgac
     1501 tactggatgg cccatgcgcg gcactaccag ctgcagttca ccagtggcga acacgacaat
     1561 cccgacggac gcatcgccga ggacatccgc atcgccaccg agtcggctat cggcctggtc
     1621 ctctccctgc tctattcgct gctgatcctc ggcagtttca tcgagatcct gctcagcgtg
     1681 tcgggcagcc tgccaatccc cggcaccagc ctcgaggtgc cgggctacct cgtcctgctg
     1741 gccttcctct atgccggtgg cggcaccgtg ctcggcctgc tgctggggcg cccactgatc
     1801 aaggccacca accgcctgca gacggtggag gccaacctgc gcttcggcct ggcccgggcc
     1861 cgggagaact ccgagtcgat cgccctgatg cacggcgaag gcattgagca ccgccaggcg
     1921 gataagttgt tcgccgacgt cagtctcggc tggaaccgcc agaccttcgg ctacctgggc
     1981 atcgtttcct tctccaccgg ctatggcacg ctgctgccgg tcttccccat ccttatcgcc
     2041 gcaccgcagt tcattgccgg ggccatgtcc ctcggcatcc tcatgcaggc ggcgcaggcc
     2101 ttccagaaac tcacttcggc gctgtcctgg ccgatcgaca accttggcga actggccaag
     2161 tgccgcgctt ccgccgaccg cgtgatcagc ctctacaacg acatgctgct gctcgagaag
     2221 cgggcctcca atccggtgga gcaccgcatc gacctgcata cgaaccgaag cgacgagctg
     2281 gcgctgcgcg gcctgagcct ggccaacccg gatggcaagg tgctgctgga aaatctcgac
     2341 ctgacgatcc agcgcggcga gcgcgtgctg atcagcggcg acccgaccct gaccatcggc
     2401 ctgttcaaag ccgtcgccgg gctctggccc tggggccatg gcgaaatcct cctgccggcc
     2461 gggcaaagca tcgtgttcct gccgcagcgc cccttcctcc cggacggcac cctgcaggcg
     2521 gcgctcagct atccgcacgc ctccggccac ttcaacaacg ccaggatcca gcatgccctg
     2581 acctgcgccg gggtcgcctg gcttgcgcca cgcctggagg aaagcgacga ctgggaccgg
     2641 gtgttgccgc tgcgtgcgca gcagcgcctg ggcatcgccc ggctgctgct gcatcacccg
     2701 gcctgggtat tcatcgagga ggccaccgac gccttcgacg tgcagggcga ggaatccatg
     2761 accgaagtgc tgcaccgcga gctgcagggc tcgacactgc tgaccatcag cttccacagc
     2821 accctggcgc acttctacac ccgcaagctg gtactcgacc gcatctgcga ggacaagtac
     2881 ctgacctgct cccccgagcc agccccctgc gcgctacgcg aaccgtgaag gcaaccagcc
     2941 gcgctccacg cactcgcgga agcaccaggc cggggtggtc tcggtcttgt agctccagaa
     3001 gaaccagccc aggtacttct cgtaggtcgc cagctggccg gcggcatagc cgcggtaggc
     3061 caccgactcc tggaacgcat ccatctgctc cagggcatgg ttgaacgggc cttcggccca
     3121 cagcgacacc accttcaggt ccagtcccag gctccactcg ccgcagaagg tcggcagccc
     3181 cagctcggtg atgatggcat cggcttcctg cttccagtcg ccggcggcct tgcggatatg
     3241 gccgtagatg tccatgtcga tatcgccgcg ctcaaagcac tggtagcggt ggatgtcgaa
     3301 gatcacgttg cggtattgcg gcgcctgcat gaagcccagg tactcgcgga acgagcggaa
     3361 gccatcatgg aacaccaccg ccacccgctc ggccgggcag tgcttgcgga tacgctcgta
     3421 ggccgccagg ttataggcct tgaggtagtc ggtgggcaca tcccagcgcg gctcgttgag
     3481 tacctcgatg gcatgcagtg ccgggtgcgc gcgatagcgc tcggccaggc gctccagcac
     3541 gcccagggaa tgctccaggt actccggctt ggtgtgccac tcgcacacgt ccttgatacc
     3601 gccgttgtcg aagccgttct ggcagcccgg tgccgcgtgc aggtcgagca ccacacgcaa
     3661 gccgaactcc tgcgcccagt ccatggcgcg gtcgagcacc tcgatgccgc cagtgacgaa
     3721 gggatggcgc tgctcgccat agctggggtg atagggatag tcggcaccga agatccagtg
     3781 ccccagcggg atacgcaccg cgttgagacc gtgttcggcg agccaggcga aatcctcacg
     3841 ggtgatgaag ctgttccagt gccggcgcag cttgtcgccg gcagcctcgc ccagttcagc
     3901 gcaccacgtg gtctcgtcag tagccgccat cccctcgaac aggctgggga ccatccactt
     3961 ttccagaacc agccaactgc ccagattgac tccgcgcaac ttcatgccgg gctgctccat
     4021 gtttcgctct cctctgccag cctgcgtcgg cgcactcgcg tgcacctatc ctatttcaaa
     4081 ttagcataag cgccggtccg gagaaggact ggcgcgcctg acgcccagat caggcaatgc
     4141 tttcgacgat cccgccctcg acccgcaggg ctgcacccgt ggtggcgctg gcttgcggcg
     4201 aggcgacata gacacacatg ttggcgatct catcgggatc ggcaaagcgt tgcagcaggc
     4261 tcgacggacg attttccttg atgaacagtg cctccatctc cgtggtcaat acgccgcgcg
     4321 cctcggccat cgaagcgagg aaattgacca cgccctcggt gcgcgtcggc cccggcagga
     4381 tgctgttgac cgtcaccccg gtacccgcca gcaccttggc cagaccgcgg gagacgccct
     4441 gcagggcggc cttgctgacg ccgtagtgca ccatctcggc cgggatgttc aacgccgact
     4501 cactggagat gaactggatc cgcccccaac cgcgttcgcg catgccctgg gcataatggc
     4561 gcgacaggcg gataccactg agcacgttga cctggaacat ctcctcccac tgatcatcgt
     4621 cgatctcgaa gaagtcgcac acggcgtaga tgccgaggtt gttgatcagg atgtccgcct
     4681 gcggctgggc ggcaatcagc tgcttgcagc cagcggcagt cgccacatcg gcaaccacac
     4741 cacgaaactt gcgggccggc acggcatcac gcaaacgggc cagcgccgca tccaggcgct
     4801 cctgatcgcg gccggtaatg accacggtgg cgccggcacg cgcgaggccc ttggcaatgg
     4861 ccaggccgat accgccggtg gcgccggtga cgatggctgt cttgtcggac agatcgatga
     4921 acatgcaaaa ctccttcatg tagcagaaaa cggaaagcct gcggcacagg atcgcgtgcc
     4981 agggtgcagc agcctaaccc gagagcatcc ccgctgggga gacaccagta acgaacaaca
     5041 cctcccccgc cggctctgtt agccgcggtc gccaggcaaa acggcccggc tgacgtcctg
     5101 tttaaccgtg ttgagtgagg cggacggtct atgtgcagca gtcaaaaata taattatgtt
     5161 aaatagcgca ccaaggccgc tcccgcatgg ccgtgcgcgc acggctgccg aatgcgcata
     5221 gcgccggctt ttcccgcagg cgccgagagg tgccgaaccc gtgcctgata cccgacgcgg
     5281 gcaacggtct acgctcaacc gatgccccgc cccttcacgg acggggcatg acgctcatcg
     5341 gaagcgagga gaacccagat ggctaccccc tacaagcgcc tcgacaagaa ccaggctgcc
     5401 gtgctgctgg tcgaccacca ggccggcctg ctgtccctgg tacgcgacaa cgacccggac
     5461 cgcttcaaga acaacgtgct ggccctgggc gacctcgcca agtacttcaa tctgccgacc
     5521 atcctcacca ccagcttcga gagcggcccc aacggcccgc tggtgcccga actcagggag
     5581 cagttccccg cagcgccgta catcgcccgc cccggccaga tcaacgcctg ggacaatgag
     5641 gacttcgttg cggcggtgaa ggcgaccggc aggaagcagc tgatcatcgc cggtgtggtc
     5701 accgaggtct gcgtcgcctt cccggccctg tcggccattg ccgcgggcta cgaggttttc
     5761 gtggtcaccg acgcgtccgg caccttcaac gaactcaccc ggcactccgc ctgggaccgc
     5821 atgtcctccg ccggcgcaca gctgatgacc tggttcggcg ttgcctgcga gctacaccgc
     5881 gactggcgca acgatgtcga gggcctcgcg gccttgttct ccaaccacat cccggactac
     5941 cgcaacctga tgaccagcta cgccacgctg gccgcggcca aatgaccgca gggcggttca
     6001 gacgtattgc gccttgccgt ccgctcagtc cttcccgcgc agcccagttc atgccagcag
     6061 ggctgcgcgg tattgccaag tcgtcgctgg tcaatcgcgg gtgatgtcgg cttctgcata
     6121 gcccgttcat ggtgaatgcc gctctgaaaa tagccatcaa gccgagactt atcgccaaag
     6181 acggccctgc aaacagtgaa tatcactact cccctctttc tatactcagc ctgagcgacg
     6241 atgcatccgg gcgcagcgga tggtggaggc aagtatgcga gttctggtgc tgggaagcgg
     6301 tgtggtgggc acggccagtg cgtactatct ggcccgcacg ggcttcgagg tggtggtggt
     6361 cgaccgtcag gatggcgctg cgctggaaac cagctatgcc aatgccggac aggtctcccc
     6421 cggctatgcc gcgccctggg ccgcgccggg catcccgttg aaagccttga aatggctgct
     6481 gcagcgccat gccccgttgg cgatccggct caccgccgac cccgcgcagt acctgtggat
     6541 ggcgcagatg ctgcgcaact gtaccgccac ccgctatgcg ctcaacaagg agcgcatggt
     6601 gcgcctggcc gagtacagtc gcgactgcct cgacgagctg cgcgccgaga ccggcctcac
     6661 ctacgaaggg cgccagctcg gcaccaccca gctgttccgc agccaggcgc aggtcgatgc
     6721 cgcagcgagg gacatcaccg tgctggagcg ggccggggtc ccctatgaag tcctcgaccg
     6781 ggccggcatc gtccgcgtcg agccggcgct ggcggcagtc gccgacaagc tggccggagc
     6841 cctgcgcctg cccaacgacc agaccggcga ctgctttctg ttcaccaatc acctggcgga
     6901 gatggcccgc acgctgggcg tcgaattccg cttcggccaa cgcatcgagc gtctggagtg
     6961 cgacggcgca cgcatccgcg gggtgtggat cgacggccag ctcgaaaccg ccgatcacta
     7021 cgtgctggca ttgggcagct actcgccgca actgctcaag ccgctgggaa tccagctgcc
     7081 ggtgtatcca ctgaagggct attcgctgac cgtgccgatc accaatgcgg ccatggcgcc
     7141 gacctcgacc attctcgacg agacttacaa ggtcgccctc acccgcttcg acaagcggat
     7201 ccgggtcggc ggcatggccg agatcgccgg cttcgacctg gcgctcgacc cgcgccggcg
     7261 cgccaccctg gaaatgatta cgaccgacct ctttccgcag ggcggtgacc tggcacgcgc
     7321 cgacttctgg accggcctgc gccccgcgac cccggatggc acgccgattg tcggcgccac
     7381 ccgctatcgc aacctgttcc tcaataccgg ccacggcacc ctgggatgga ccatggcctg
     7441 cggctccggc cgcctgctgg ccgacctgat ggccgcgcgc cgcccgcgga tcgacccgca
     7501 atgcctcgac atcggccgct atggccgcga gcagcgcacg ctccagccgt cccatccaac
     7561 accagcccac tgattgaccg gcatcagcag gctgactgtc agacggtgtt cggaacgcta
     7621 gctgacctca agcgccgggt gtaaaccagg ccccaggtca gaccgaggat ggcggctatg
     7681 gccgaggcca tcagcacccc caacttggcg gcgctgagca aggtctcact gctgaacgcc
     7741 agggtcgcga tgaagatgga catggtgaag ccgatgcccg ccagcaggcc aaccaggcag
     7801 acaccggacc atgtcacttc aggtggcagg cggcaccagc ccaggcgcac cacgcaccag
     7861 ctggccacga tcacgcccag gggtttgccc acgaccaggg ccagcaacac gcccagaata
     7921 acgcccagcg agccatcggc ctgcaaatcg atgccatcca gccccacccc cgcattcgcc
     7981 aaggcaaaca acggcatgac cccgtaggca acccaggggt gcagcgccat ctgcacgcgg
     8041 gtgacggggg gcagcaggtc gcgttgtgcc ttgcgcaact ccttgagaga ttgcacgaac
     8101 tggccctcct gcggatgggg agtcgtcgca cgctcgcaca ggtcactgac ggcacgggtc
     8161 atcgcgtcca gcggccgcag ctgtgaacgc cccggcagga ccggggtcat caggcccagc
     8221 acgacaccgg ccagggtcgg atgggcgccg gtcttcagga aaccaagcca cagcacggcc
     8281 cccggaatga tgtaggcgta agccgagccg ataccgatcc actgaagggc gagaaccatc
     8341 aggatgccga tgccggcgac cagcaatccg gtttgatcga gcccgccgct gtagaacacc
     8401 gcgatgatca acaccgcgat gatgtcatcg atgatggcca gcgccagcag gagcacacgc
     8461 acgttaccgg ggatggagcg gcccagcagg gcgagtacgc cgacggcgaa tgcgatatcg
     8521 gtggcggttg gaacggccca gccctgccgc ggcaccggct cgccattcag tgcgagataa
     8581 atcagcgcgg gaaccatgac accaccgagg gcggcaacga taggcagcga ggccagccgc
     8641 caactggaca gggcaccctc gtggatctca cgacgaatct ccatgcccac gaccaggaag
     8701 aatacggtca tcagggcatc gttgatccag aagtgcagcg actgggaaac gacgtaactg
     8761 cccaggccaa aagacagtgg cgcatgccac aacgcgtggt agctatcggc ggcaggtgaa
     8821 ttggcccaaa tgagcgccgc tgctgcagct accagcagca cgatgccact gacagcctca
     8881 atatgcagga agcgttcgag cgtcgagaag gctcgttcag ccaaaagctg ggcgcgcgga
     8941 attgcctggc gagaagagtg tcggtccatg gtcatagaat accggcgcag gcggcccgac
     9001 ctgcgcattg acctgtttca ctgcgacatg cagtgctaac acccgcaatt acaatacaaa
     9061 aaagctgcac ccgcacacaa gccaaaaggc cacggcgcct gatgcatgac aggagcaccc
     9121 gcggcattcc aaggcgttgg cgagcgcctg cgtcgcatcc cgttacaaca atcaagcagc
     9181 ccgatgtaga tctttgaccg gctgacctgt tgtcgcacgg gttgactgcc atcgtcctgc
     9241 cgcccctcgc gcaatgcgct tcgccaagcg tgacccaagg ctgcattgtc ggtcttgatt
     9301 tttatctgta tgcatataca gtacatctac accgacgact catccactcg gcacagaccg
     9361 aacctcacat cgatgccagc aagaaggagc ccaacgatgt ttttttcgac acttggtact
     9421 gctctctccc tgaggaaggc taccccctac gatctgaatg atttcctgtt ctcgcccagc
     9481 gaactgcagc acaagtacga aacccgggtg cgtcacagcg aacacccgca tttcgaccgt
     9541 gccctgtggt tgcgtgcagt caatgccggc gcttgcgagc tcggttactg gcagtgggtc
     9601 ttcgaacagg tccaggctga tgacgatacc tacctactgc gccactgacg cgaagctcta
     9661 ccccgccagc acaggatacg ggccccctcg cgcgggcccg gttcgcaggc cactgtctcg
     9721 cacaactgat cgtcgcagct catctgcgca atcgcgcgcg cccccccttg cccaggcagc
     9781 gtcaccggcc gagggccggg atcgccgtcg ctggataccg cgccagccag atgtgcggtg
     9841 gaaccccgcg caacgcgcaa aaatcccacc gacatcccgg tctggcggac ggcatgggga
     9901 ttcagtcggc cgccagcatg tgcccgcgct ggcccaggtt gatgtgccag gacagggcct
     9961 cctccagcag gtgcggcgtc tggccgccac gctcgcaggc cgccgcgaag tagccgccca
    10021 gcgcctcgcg atagtccgga tgcacgcagt tgtcgatgat cacccgggcc cgctcgcgcg
    10081 gtgccaggcc gcgcaggtcg gccaggccct gctcggtgac caggatgtcg acgtcgtgct
    10141 cggtatggtc cacgtgactg accatcggca ccacgctgga gatcgccccg tccttggcaa
    10201 tcgacttggt gacgaagatc gccagctgcg cattgcgggc gaagtcgccg gagccgccga
    10261 tgccgttcat catgtgcgtg ccgcccacgt gggtggagtt gacgttgccg tagatatcga
    10321 actccagcgc ggtgttgatg ccgatgatgc cgaggcggcg ggccacttcc ggatggttgg
    10381 agatttcctg cggacgcagc accaggcgct ccttgtagcg cgcgaagtcg ccgaacacct
    10441 gggcgccgcg cgtctcggag aggacgatcg cgctgcccga ggcgaagtcc agcttgccgg
    10501 cgtcgaacag ttcgaaggtg gagtcctgca gcacc
//
LOCUS       DATCUN010000273        15061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_163235, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000273 DATCUN010000000
VERSION     DATCUN010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 15061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15061
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163235"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1067)
                     /locus_tag="VIO83_10635"
     CDS             complement(111..1067)
                     /locus_tag="VIO83_10635"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8331769.1"
                     /translation="MSEVFDIVVVGSGHNGLTAAGYLAKAGKSVLVLESKPYFGGGVD
                     TREITVPGFRHDLHSSCHMGVVINPLISKDELQLQRKYGLEYIRPDADYSTTFSDGNC
                     LISYRDLDRTLDSIASVSRKDADAYRRLYQDSIDFISLAIEGMFVPPPPFGAVVALLD
                     QSAMGRNALHELQRSPLDILDERFESEKVKIHMMRLLTEHFVDPECMGEGGALFMYPA
                     FLDRYGLNTPRGGSGGLVDALVRQLQDHGAVLRADSTVVKVLTAKGRAEGVRLADGTE
                     IRAREAVVGQIHPYNLSAMVDGLDDQILYEARRAQHSSRARI"
     gene            complement(1377..1628)
                     /locus_tag="VIO83_10640"
     CDS             complement(1377..1628)
                     /locus_tag="VIO83_10640"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8331770.1"
                     /translation="MGISRRYLDKVLADADTTFLTLLENHRLEAARLRLGDRHAARQI
                     SEVAWSCGFTDPGYFARRFRRSFGISPPRLRCIVSEGNG"
     gene            complement(1671..2354)
                     /locus_tag="VIO83_10645"
     CDS             complement(1671..2354)
                     /locus_tag="VIO83_10645"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331771.1"
                     /translation="MRESTTALSRDADWLWSSEGISFRQALEETQKLSACLISRSRVS
                     GESYRYRIAWQHYGMGALELNFMSGATQRVMRTRSMIDHDRNNDFHLVLMQAGGARMI
                     HDGSDCELAPGAMILLNCAKSWDLMFPSSRLCLSARMDFRWLRRWLPDPSALAGRVVG
                     AGDPWGAPMVEMMKAIAAEGIENAPMPHALLADQMAGLLVLALAPREDVQARHGAELL
                     RRIRAVIRD"
     gene            complement(2475..3113)
                     /locus_tag="VIO83_10650"
     CDS             complement(2475..3113)
                     /locus_tag="VIO83_10650"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8331772.1"
                     /translation="MSEKPVRRGRRNKSADVLSAACRQFSRRGFDGTNMDSVAASARV
                     TKMTVYANFGSKSGLFAAALEELKRDMPAPEDLMRCAGRDLAEDLRGVAIRLLEVCLR
                     SSTLGIYRMLIFPLESDPYLGRNFWRGTIEPYHEAMRRILREADRRGVLTIADPACAS
                     SLFFSLVIGEPTLRMLLGAECAMSRRARTAHVKVAVDLFMAGHGRSPRTVAR"
     gene            complement(3221..3811)
                     /locus_tag="VIO83_10655"
     CDS             complement(3221..3811)
                     /locus_tag="VIO83_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8331773.1"
                     /translation="MTASKKSAETAIARRRKEQVLAAAAECVRREGFHRTSMSQISAA
                     AGMSSGHIHHFFGGKEGIIAGIVAREHSELGQLIEDVKDASRSTDAVSAIVEHVPRSA
                     ASYMENGHAALTMEILAECSRNPEVASLIQRNDLEVRSAFHELLGGSTPEVQSRCEIV
                     AALLEGLSARALRNPRLAASLDQAMLERVVRFALSG"
     gene            4236..5384
                     /locus_tag="VIO83_10660"
     CDS             4236..5384
                     /locus_tag="VIO83_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004346590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8331774.1"
                     /translation="MNNRRTPLAVSLALIAACVSLSACKKDGGASDAPPPPPEVGVLT
                     LAPQSLHLSTELPGRTTAYLVAEVRPQVSGILLERKFQEGSDVKAGQVLYQINPAPFR
                     ATLARAEASLEAARLLSERYDRLIQTRAISQQERDDARSQFLQARAAVESARIDLDFT
                     RIAAPISGRIGRSAVTQGALVTANQADALSTVQQLDPIFVDVVQPSAALLRLRADLAS
                     GRLKSAGKGQAEVRLKLENGQDYPHAGKLQFSEVSVDPSTGAVTLRAVFPNPDGVLLP
                     GMFVRAQLQEGVREQALLVPQRGVSRDSQGKAMALVVDSKNIVQPRELTTERSVDGQW
                     LVSDGLQAGDRVVVDGVQLARPGAEVKPVPAASTDAAAKAPATPEAGK"
     gene            5394..8525
                     /locus_tag="VIO83_10665"
     CDS             5394..8525
                     /locus_tag="VIO83_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8331775.1"
                     /translation="MSRFFIDRPIFAWVIAIVIMLAGGLSILALPVNQYPNIAPPAIG
                     IIASYPGASAQTVQDTVTQVIEQQLNGLDRLRYIRSESNADGSVTIVVTFEQGVDPDI
                     AQVQVQNKLSLATPMLPQAVQQLGLRVVKYQVNFMLVAALVSEDGRLDNFALGDLIVS
                     QLQDPLTRTPGVGDFFVLGAQNAMRVWLDPMKLNSYQLTPSDVSAAISAQNVQVSSGQ
                     LGGLPTGDKVQLNATVIGKTLLQTPEQFGAILLKVNPDGSQVRLRDVADVALGADNFS
                     ISARYNGQPSAGIALRLASGGNTLNATSAVRETLGRLEPTLPPGVKVVYPYDTAPVVS
                     ASIEGVAHTLVEAIVLVFIIMYLFLQNFRATLIPTLAVPVVLLGTFGVMSAFGFSINT
                     LTMFGLVLAIGLLVDDAIVVVENVERLMVEEGLSPLEATRKSMDQISGALVGIGLVLS
                     AVFIPMAFFGGSTGVIYRQFSLTIVTAMTLSVLVALIFTPALCATLLKPVHGHHEKKG
                     FFGWFNRVFDRSSQRYERGVSRVIGGRGRYMAAFVLIIVALALLFPLMPTSFLPDEDQ
                     GTMLAQVELPPNSTAEQTDRLLREVSDYLLEEEGGSVESVFAVNGFSFAGRGQNSGIA
                     FVQLKPWEEREGTVFDLQARAMQRFSQMKAGTALAFAPPAIQELGNATGFNLFLQDYS
                     GRGHENLMQARGQFLAEAAKHPALALVRPNGKGDEPQYQVLIDDEKARTLNVDIAEIN
                     ATMSAAWGSSYINDFIDRGRVKRVYIQGIPQARITPEDFDKWFVRNNDGQMVSFASFA
                     TGKWVYGSPKLERYNGVPAIEILGEPAPGYSSGDAMRAVEEIAAKLPDGITLAWTGLS
                     YEERLSGSQAPALYALSIIAVFLCLAALYESWSIPFSVMLVVPLGVLGTVAATLMRGL
                     ENDAFFQIGLLTTVGLCAKNAILIVEFAKELHEKGGRTLVQAAVEASRLRLRPIIMTS
                     LAFTMGVIPLAIATGASSGSKHAIGTGVIGGMVSATFLAIFFIPLFYVVVSSFFKKKN
                     RVESIPATEEPSA"
     gene            8522..10015
                     /locus_tag="VIO83_10670"
     CDS             8522..10015
                     /locus_tag="VIO83_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008075449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY8331776.1"
                     /translation="MMTFPLRAVLPALIGATLSGCVSMAPNYERPAAPVATQWPEQAA
                     APATAVSGASAGDIPWQAVFADPQLRQVVQMALDNNRDLRVAVLNIDRARAQYRIQRA
                     DLLPEVGAGVSGNNGRTPASISPTGTATTSHTYRADLNVAWELDLFGRVRSLSDQALQ
                     QYFATEAAQRATRIGLITETATAYLTLASDTEQLALARETLRSRQEAYDLQLALRDTG
                     NASDLALRQAESELEAARDQMLLLDGSVATDRNALELLVGAPLPEDLRPQAPLESMLA
                     VQDLPAGLPSDLLQRRPDILAAEHQLMGANANIGAARAAFFPSISLTAGVGRASDSLS
                     SLFDGGNRTWSFLPQISVPIFSGGRLRANLAVSEIDRDIAVADYERSIQTAFREVADA
                     LAIRNRIDGRLTAQERRVAAARDAYALVQQRYDNGVSRYLDVLDAQRTLYTAQETWIS
                     TRLTRQINLVSLYKALGGDFAQNAPASDVAAGPRIHATQGDQLAARP"
     gene            10035..10877
                     /locus_tag="VIO83_10675"
     CDS             10035..10877
                     /locus_tag="VIO83_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8331777.1"
                     /translation="MLKDIAVFYDVSHEGKHILETAAVLAEKQNARLIGITATNSSDI
                     ESYPSDSFARGAGIGEVISRLQSSTAAHLLHAAQALKEVADRHGVTAEFRAIPWSESG
                     VEMSLHSLYCDLLVVNHPKAPGAPFAWSSIDVLQQTGVPLLIVPQSWRSIQLGQRITV
                     AWNASRQARRAVADALPLLISAEAVDLLIVDPERKAELHGEEPGADMGSYLARHGVRV
                     NLERIDSADKPVADVILEHAVKAKADLIVFDAYSRPKLSELVLGGVSRTLLSSVPLPL
                     FVSH"
     gene            11045..11569
                     /locus_tag="VIO83_10680"
     CDS             11045..11569
                     /locus_tag="VIO83_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331778.1"
                     /translation="MVVSYFPEPHQLLDEVLKRGRLSLVDDLQSVFDAQMRVMPALRS
                     ALQLCLNEVVHNDRVRAAQEILAFHCDFFTTQRNLLEEQIVHFARVMGLLQKIAIEAA
                     KGGELRDGVRPDAWALIIGLTLSGAIRLCVMQRTDDGRLACWSEATRALDEAFSFVAP
                     GPRHRLAPSQRKNG"
     gene            11574..12185
                     /locus_tag="VIO83_10685"
     CDS             11574..12185
                     /locus_tag="VIO83_10685"
                     /inference="COORDINATES: protein motif:HMM:NF019960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY8331779.1"
                     /translation="MARRKKEDSLETRERILDAAEWCFCTYGVSQSTLALIAEKAECT
                     RGAIYWHFPKEAEVLKLVLDRGRLSLQRRLASLTHAQPPLMPKLQVCLDDYLAEVSRN
                     RHVRSTLKILLLRCDYVGLRQAFVGRRYVEALEIYGPLVDLLARAHAAGEMAPELDPR
                     NGAELLQCLLIGGLRKWLIDSMDGDESSPRDILSLALGLLRVR"
     gene            12461..12697
                     /locus_tag="VIO83_10690"
                     /pseudo
     CDS             12461..12697
                     /locus_tag="VIO83_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003158902.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(12754..14697)
                     /locus_tag="VIO83_10695"
     CDS             complement(12754..14697)
                     /locus_tag="VIO83_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEY8331780.1"
                     /translation="MALSDLTVRQARTTGKRYTLSDNDCLGLMVSAAGGKSWIFRYYW
                     LGKQKRMSLGGYPALSLREARAERDKAQALLARGIDPQIERDQRRHAARLAGEYTFKN
                     VFDAWVEHRRKELKEGRQSTLSQILRIFNKDVLPTLGKMSIYDIRRPQLVGVVAAIEK
                     RKAFTTAEKVRTWFNQMFRYALVIAEGLEVNPAADLDVVAEPKPPVAHNPYLHLPELP
                     EFLQKLRLYNPRGWQTQLGVRLLFLTGVRTGELRLAEPEQFDLDRGLWIIPPQVVKQL
                     QDEMRKAGKRPQDVPPYIVPLSLQAIEIVRYLLGVMRPAQKYLLSHRSELKKRISENT
                     LNKALQLMGYEGRLTGHGIRGTISTALNEIGYPKIWVDAQLSHSDPNKVSSAYNHAKY
                     VEPRRRMMQDWGDRLDLLEQGEVEAASAHLTIRIDGVPAMAEVEEAVGAVPTVAEPAV
                     PGVPPVVATPIVVTPNSGEITFQRLSQVPPPPTHAPEPEVSSIQREREEMLAVYESPN
                     NLPVPLFGKLAGKSKDQINRELKAGKLLSISLGNRGQRVPDWQLVPLKHKLAQVLMNQ
                     CPHADSWDLYRMLTQPHPDLGDRAAIDAVTPTNVPAVIRVIMGDYQHHADVPETVATQ
                     PIPEDVRQSVRRLVDSAVVFERA"
     gene            complement(14924..14999)
                     /locus_tag="VIO83_10700"
     tRNA            complement(14924..14999)
                     /locus_tag="VIO83_10700"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14964..14966),aa:Gly,seq:gcc)
BASE COUNT         2571 a         5021 c         4831 g         2638 t
ORIGIN      
        1 cgtggctacc acgggcggca cgccagggac agcgggctcg gctaccgtgg gaaccgcgcc
       61 caccgcttcc tccacttctg ccatcgccgg caccccgtcg atacggatcg tcagatgcgc
      121 gctcgcgacg aatgctgcgc gcgcctggcc tcgtacagga tctgatcgtc caggccatcg
      181 accatcgcgg acaggttgta cgggtggatc tggccgacca cggcctcccg ggcgcggatc
      241 tcggtgccgt cggccagacg cacgccctcg gcccggccct tggcggtcag caccttcacc
      301 acggtcgaat cggcgcgcag caccgcgccg tggtcctgca actgccgtac cagggcgtca
      361 accaggccgc cgctgccgcc gcggggcgtg ttgagcccat acctgtccag gaacgccgga
      421 tacatgaaca acgcgccgcc ctcgcccatg cattccgggt cgacgaaatg ctcggtcagc
      481 aggcgcatca tgtggatctt gaccttctcg ctctcgaaac gctcgtcgag gatgtccagc
      541 ggactgcgct gcagttcatg cagcgcgttg cggcccatgg cgctctggtc cagcagcgcc
      601 acgacggcgc cgaacggcgg cggcggcacg aacatgccct cgatcgccag cgagatgaag
      661 tcgatgctgt cctggtagag cctgcggtag gcgtccgcat ccttcctgga cacgctggcg
      721 atgctgtcca gggtgcggtc cagatcgcgg tagctgatca ggcagttgcc gtcgctgaag
      781 gtggtcgaat agtcggcgtc cgggcggatg tattccaggc cgtacttgcg ctgcaactgc
      841 agctcgtcct tgctgatcag cggattgatg accacgccca tgtgacagga cgagtgcagg
      901 tcgtgcctga aaccgggaac cgtgatctcc ctggtgtcga cgccgccacc gaagtacggc
      961 ttgctctcca gaacgagtac cgacttgccg gccttggcaa ggtagccggc tgcggtcagg
     1021 ccattgtggc cgctgccgac tacgacgata tcgaagactt cggacactgg gtttctcttc
     1081 aagctgggaa tcgacctgct ctgcggcccc gccgggaatg cgaggggcat ccacgcaaag
     1141 acgcaggtgc gaggagattc tcggccttgg ccttactcag ccatgaggac gatcgcgcac
     1201 aggatctggg acgatacggc gcccggcgcc gctggccccg ggcattccgg tggccgatgg
     1261 cacagcgtgg ccgcagccat gcacagcggc cggtacggat cgctggtcac aaggcgtggc
     1321 gtggcccggc cgaaggccgc cggggcatga tgaggccctc ccgggccaac cgagggtcag
     1381 ccgttgcctt cggacacgat gcagcggagt cgcgggggcg agatcccgaa gcttcggcgg
     1441 aaccgacgcg cgaaataccc tggatcggtg aacccgcagg accaggccac ctcggagatc
     1501 tggcgcgcgg catggcggtc gcccagccgc agccgcgcgg cctcgagccg atggttttcc
     1561 agcagggtca ggaaggtcgt gtccgcatcg gcaagcacct tgtccagata gcggcgcgag
     1621 atgcccactt cctgcgcgac ctcgcgcgcc gtcagacccg gcgcatcgaa tcaatcgcgg
     1681 atgacggccc ggatacgcct cagcaattcc gcaccgtggc gcgcttggac atcctcgcgt
     1741 ggtgccagtg ccagcaccag gaggcccgcc atctggtcgg ccagcagtgc gtgtggcatc
     1801 ggcgcattct cgatgccctc ggccgcgatg gctttcatca tctcgaccat gggcgcgccc
     1861 catgggtcgc ctgcgcccac cacccgccct gccagagcgg agggatcggg cagccacctg
     1921 cgcagccagc ggaaatccat ccgcgccgaa aggcacagac ggctcgacgg aaacatgagg
     1981 tcccaggact tggcgcaatt gagcaggatc atggcgccgg gagcaagctc gcagtcgctg
     2041 ccgtcatgga tcatccgcgc gccgccggcc tgcatcagca ccaggtggaa atcattgttc
     2101 cggtcatgat cgatcatcga tcgggtgcgc atcacccgtt gcgtggctcc gctcatgaag
     2161 ttgagttcga gggcgcccat cccgtagtgc tgccaggcga tccgataccg gtacgattcg
     2221 ccgctcaccc ggctgcgcga aatcaagcag gcgctgagct tctgagtttc ctccagcgcc
     2281 tgcctgaagg agatgccctc ggacgaccat agccagtctg cgtcacggga cagtgccgtc
     2341 gtagattcgc gcatatcaca ccgggcagtt gctcgccgga agcatgccgg ctcgtccctc
     2401 atccgacacg ctgcactgca aaaaccggac ggggaccggc catgcgtagc cgagctggcc
     2461 aggcgctcgc gctcctagcg cgccacggtg cgcggcgatc gcccgtggcc ggccatgaac
     2521 agatcgaccg ccactttcac gtgagccgtt ctcgccctgc gtgacatcgc gcattccgcg
     2581 cccagcagca tgcgcagcgt cggctccccg atcaccagac tgaagaacag ggacgacgca
     2641 caggcgggat cggcgatcgt cagcaccccg cggcgatccg cctcgcgcag tatccgccgc
     2701 atcgcctcgt gatagggctc gatcgtgcct cgccagaagt tcctccccag gtacgggtcc
     2761 gattcgagcg ggaagatcag catccgatag atccccagcg tcgaacttcg caggcacacc
     2821 tccagcagtc ggatcgcgac gcccctcaga tcctcggcaa gatccctgcc cgcgcaacgc
     2881 atcaggtctt ccggcgccgg catgtcgcgc ttcagctctt ccagcgccgc cgcgaacaga
     2941 ccgctcttgc tgccgaagtt ggcatagacc gtcatcttgg tgacccgcgc cgaggcggcc
     3001 acgctgtcca tgttggtgcc gtcaaacccc cggcgcgaga actgccggca tgcggcgctc
     3061 aacacatccg ccgacttgtt gcgtcgtccg cgtcgcacgg gcttttcact catgccgggc
     3121 atgctcgtgc ccctccctac catgattggg ccatgacgcc atgcgccagc ggcgaagcca
     3181 ccgtcgccgc ggcggcttcg atccgtcgac gccgagcgtg tcagccgctg agcgcgaaac
     3241 ggaccacccg ttccagcatg gcttgatcga gcgaggccgc cagcctggga ttgcgcagcg
     3301 cacgcgctga caatccctcg agcagcgcag cgacgatctc gcagcgggac tgcacctccg
     3361 gcgtgcttcc gcccagcagt tcgtggaacg cggaacgcac ttccaggtcg ttgcgctgga
     3421 tcaggctggc gacttccgga ttgcggctgc attcggccag tatctccatc gtcagcgcgg
     3481 cgtggccgtt ctccatgtaa ctggccgcac tcctgggcac gtgctcgacg atggccgaca
     3541 ccgcatccgt actgcgggag gcgtccttga cgtcctcgat gagctggccc agctcgctgt
     3601 gctcacgcgc cacgatgccg gcgatgatgc cttccttgcc gccgaagaag tgatggatat
     3661 ggccggagct catcccggcc gccgcggaga tctgcgacat gctggtgcga tggaagcctt
     3721 cgcggcgcac gcattcggcc gccgccgcga gcacctgctc cttgcgtcgc cgggcgatcg
     3781 ccgtctcggc ggatttcttg ctggctgtca tttccgtttc ctcttgaaga actggagcct
     3841 gccgacccgc ttgctgcgcc ggtcggctgg cgccggtgcg gtgcgcgcaa ccaccgtcgg
     3901 caacggtgcc tggcagggcc gcctcagcac gcgcacagac cggatctacg ccctgctccg
     3961 cttcacaaca ctggaaccta tgcgctcgcg aggaaccgtg aacaggcgct tgcagcccgc
     4021 gctctggggg cgattcagcg tcgcctgaaa gatttttctt gcgccgcagc acaaggttgt
     4081 agtgtacact agatccaggt gggtcgtgca acctagatat ctggttggcg cctgcgaccc
     4141 acacacactt cttaactttg ctcacgtcct ggacggccgt cctgttcgcc gcggaaaagt
     4201 taggttggtc aagccaccta gattaagagt catctatgaa caacagaaga acccccctgg
     4261 cggtcagcct cgcattgatc gctgcctgtg tgtccctgtc ggcctgcaag aaggatggcg
     4321 gggcctccga cgcacctcca ccgccaccgg aggtcggcgt gctcacgctt gcgccgcagt
     4381 cgctgcatct atcgaccgaa cttcccggcc gcaccaccgc gtatctggtc gcggaggtcc
     4441 ggccgcaggt gagcggcatc ctgctcgaac gcaagttcca ggagggcagc gacgtcaagg
     4501 ccggccaggt gctctaccag atcaacccgg cacccttccg cgccacgctc gcgcgcgccg
     4561 aggccagtct cgaggcggcg cgcctgctgt ccgagcgcta cgaccgcctg atccagaccc
     4621 gcgccatcag ccagcaggag cgcgacgacg cccgttcgca attcctgcag gcgcgggcgg
     4681 cggtggaaag cgcacgcatc gatctggact tcacccgcat cgccgcgccg atctccggcc
     4741 gcatcggccg ctcggcggtc acccagggcg ccctggtgac cgccaaccag gccgacgcgc
     4801 tgtccacggt gcagcagttg gacccgatct tcgtcgacgt ggtgcagcct tcggccgcat
     4861 tgctgcggct gcgcgcagac ctggccagcg gccggctcaa gagcgcgggt aaaggccagg
     4921 cggaagtccg cctgaagctg gagaacggcc aggactaccc gcatgccggg aaactgcagt
     4981 tctccgaagt cagcgtggac cccagcactg gcgcggtgac gctgcgcgcg gtgttcccca
     5041 atcccgacgg cgtgctgctg ccgggcatgt tcgtgcgtgc gcagctgcag gaaggcgtgc
     5101 gcgagcaagc gctgctggtg ccccagcgcg gcgtgagccg cgacagccaa ggcaaggcca
     5161 tggcgctcgt ggtggactcc aagaacatcg tgcaaccgcg cgagctgacc accgaacgca
     5221 gcgtcgatgg ccaatggctg gtcagcgacg gcctccaggc cggcgatcgg gtcgtcgtgg
     5281 acggtgtcca gctcgcccgc cctggcgccg aggtcaagcc cgtgccggcc gcgtccaccg
     5341 acgccgcagc caaggcaccg gccacgcccg aagcgggcaa gtagggaaac cccatgtcca
     5401 gattcttcat cgaccgcccc atcttcgcct gggtcatcgc catcgtcatc atgttggccg
     5461 gcggcctgtc catcctcgcc ctgccggtca accagtaccc caacatcgcc cccccggcca
     5521 taggcatcat cgcaagctat cccggcgcct cggcgcagac ggtgcaggac accgtcaccc
     5581 aggtgatcga gcagcagctc aatggcctgg accggctgcg ctacatccgc tcggagagca
     5641 acgccgacgg cagcgtcacc atcgtggtga ccttcgagca gggcgtggat ccggacatcg
     5701 cccaagtgca ggtgcagaac aagctgtccc tggccacacc gatgctgccc caggccgtgc
     5761 agcagctcgg cctgcgcgtg gtcaagtacc aggtcaactt catgctggtg gccgcgctgg
     5821 tatccgagga cggacggctg gacaacttcg ctctgggcga cctgatcgtc tcgcagctgc
     5881 aggatccgct cacgcgcacg cccggcgtcg gcgacttttt cgtgttgggc gcgcagaacg
     5941 ccatgcgcgt ctggctcgac ccgatgaagc tcaacagcta ccagctcacg cccagcgacg
     6001 tctcggccgc gatcagcgcg cagaacgtcc aggtctcctc cggccagctg ggcggcctgc
     6061 ccacgggcga caaggtacag ctcaacgcca cagtgatcgg caagaccctg ctgcagacgc
     6121 ccgagcagtt cggcgcgatc ctgctcaagg tcaaccccga cggctcccag gtgcgcctgc
     6181 gcgacgtcgc cgatgtcgcg ctcggcgccg acaacttctc aatcagcgcg cgctacaacg
     6241 gccagcccag cgccggcatc gcgctgcgcc tggccagcgg cggcaacacg ctgaacgcca
     6301 ccagcgcggt gcgcgagacc ctcggccggc tcgaaccgac gctgccgccg ggcgtcaagg
     6361 tggtctaccc ctacgacacc gcgccggtgg tcagcgcgtc gatcgagggc gtggcgcaca
     6421 cgctggtcga ggccatcgtg ctggtgttca tcatcatgta cctgttcctg cagaacttcc
     6481 gcgccacgct gatcccgacc ctggcggtgc cggtggtgct gctgggcacc ttcggcgtga
     6541 tgtcggcgtt cggcttcagc atcaacacgc tgaccatgtt cggcctggtg ctggcgatcg
     6601 gcctgctggt cgacgacgcc atcgtggtgg tggagaacgt cgagcggctg atggtcgagg
     6661 aagggctatc gccgctggag gccacgcgca agtccatgga ccagatctcc ggagcgctgg
     6721 tcggcatcgg cctggtgctg tcggcggtgt tcatcccgat ggcgttcttc ggcggctcca
     6781 ccggcgtgat ctaccggcag ttctcgctga ccatcgtcac cgcgatgacg ctgtcggtgc
     6841 tggtggcgct gatcttcacc ccggccctgt gcgccaccct gctcaagccg gtgcacggcc
     6901 accacgagaa gaagggcttc ttcggctggt tcaaccgcgt cttcgaccgc agctcgcagc
     6961 gctacgagcg cggcgtgagc cgcgtgatcg gcgggcgcgg gcgctacatg gcggcgttcg
     7021 tgctgatcat cgtcgcgctg gccctgctgt tcccgctgat gccgacttcg ttcctgcccg
     7081 acgaggacca gggcacgatg ctggcgcagg tcgagctgcc gcccaacagc accgccgagc
     7141 agaccgaccg gctgctgcgg gaggtgtcgg actacctgct cgaggaggaa ggtggcagcg
     7201 tcgagtccgt cttcgcggtc aacggcttca gcttcgccgg ccgtggccag aattccggca
     7261 tcgcgttcgt gcagctcaag ccgtgggagg agcgcgaggg cacggtgttc gatctgcagg
     7321 cgcgcgcgat gcagcgcttc agccagatga aggcgggtac cgcgctggcc ttcgcaccgc
     7381 cggcgatcca ggagctgggc aacgccaccg gcttcaacct gttcctgcag gactacagcg
     7441 gacgcgggca tgagaacttg atgcaggcgc gcgggcagtt cctcgccgag gccgccaagc
     7501 atcccgcgct ggcgctggtg cgccccaacg gcaagggcga cgagccgcag taccaggtgc
     7561 tgatcgacga cgagaaggcg cgcacgctca acgtggacat cgccgagatc aatgcgacga
     7621 tgtccgcggc ctggggctcg tcctacatca acgacttcat cgaccgcggg cgcgtcaagc
     7681 gcgtgtacat ccagggcatc ccgcaggcgc gcatcacccc ggaagacttc gacaagtggt
     7741 tcgtgcgcaa caacgatggc cagatggtgt ccttcgcctc ctttgccacc ggcaagtggg
     7801 tgtacggctc gcccaagctg gagcgctaca acggcgtgcc ggcgatcgag atcctcggcg
     7861 agccggcgcc cggctacagc tccggcgacg ccatgcgcgc ggtcgaggag atcgccgcca
     7921 agctgcccga cggcatcacc ctggcctgga ccgggctgtc ctacgaggag cggctgtccg
     7981 gttcgcaggc gccggcgctc tatgcgctgt cgatcatcgc cgtgttcctg tgcctggcgg
     8041 cgctctacga gagctggtcg atcccgttct cggtgatgct ggtagtgccg ctgggcgtgc
     8101 ttggaacggt ggccgccacc ttgatgcgag ggctggagaa cgacgcattc ttccagatcg
     8161 gcctgctcac cacagtcggc ctgtgcgcca agaacgccat cctgatcgtc gagttcgcca
     8221 aggaactgca cgagaagggc ggccgcacgc tggtgcaggc cgcggtcgag gcgtcgcggc
     8281 tcaggctgcg tcccatcatc atgacctcgc tggccttcac catgggcgtg atcccactgg
     8341 cgatcgccac gggcgccagc tccggcagca agcatgccat cggcactggc gtgatcggcg
     8401 gcatggtgtc cgcgaccttc ctggcgatct tcttcatccc cttgttctac gtcgtggtct
     8461 cgtcgttctt caagaagaag aaccgcgtcg aatcgatccc ggcgacagag gagccttcag
     8521 catgatgacc tttcctctac gtgccgtact accggccctg atcggagcca cactgtcggg
     8581 ttgcgtcagc atggcgccca actatgagcg gcccgccgct ccggtcgcta cgcagtggcc
     8641 ggaacaggcc gccgcccccg ccacggccgt ctccggcgct agcgccggag acatcccgtg
     8701 gcaagcggtc tttgcggatc ctcaactgcg ccaggtggtg cagatggcgc tggacaacaa
     8761 ccgcgacctg cgggtcgctg tgctcaacat cgacagggcg cgagcgcaat accgcatcca
     8821 gcgggcagat ctgctgcccg aggtcggtgc tggcgtctct ggcaacaacg gccgcacgcc
     8881 cgcgtcgatc tccccgacgg gtacggccac gaccagccac acctaccgcg ccgacctcaa
     8941 cgtcgcctgg gagttggacc tgttcgggcg cgtgcgcagc ctgtccgacc aggcactgca
     9001 acagtacttc gcaaccgagg ccgcgcagcg ggcgacccgg atcggcttga tcaccgaaac
     9061 ggccactgcg tacctgaccc tggcgtcaga caccgagcag ttggcgttgg cccgagaaac
     9121 cttgcgtagt cgccaggaag cctacgacct gcaactggcg ctacgcgaca caggcaatgc
     9181 ctcggacctc gccctgcgcc aggccgaatc cgaactcgag gcggcgcgcg accagatgtt
     9241 gttgctcgat ggcagcgtgg cgaccgaccg caatgcgctg gaactgctgg tcggagcgcc
     9301 cctgcctgag gacctgcgcc cgcaggctcc gctggagtcg atgctcgcag tgcaggacct
     9361 gccggcggga ctgccgtcgg atctgctgca acgccggcct gacatccttg cagccgagca
     9421 tcaactgatg ggcgccaatg ccaatattgg cgccgcacgg gccgcattct tcccgagcat
     9481 cagcctgacc gccggagtcg gcagggcgag cgattcgctg tcgagcttgt tcgacggggg
     9541 caaccggacc tggagcttcc tgccccagat cagcgtcccg atcttcagcg gtggccggct
     9601 gcgcgccaac ctggcggtct ccgagatcga ccgggacatc gcggtggccg actacgagcg
     9661 cagcatccag accgcgttcc gcgaggtggc cgatgccctg gccatccgca atcgcatcga
     9721 cggccgcctc acggctcagg agcgccgcgt cgcggccgca cgcgatgcct atgcgctggt
     9781 ccagcagcgg tacgacaacg gggtgtcccg ctacctggac gtcctggacg cccagcgcac
     9841 gctttacacg gcacaggaga cctggatttc cacccggctg acccgacaga tcaacctggt
     9901 cagcctctac aaggccttgg gcggcgactt cgcccagaac gcgccggcga gcgacgtcgc
     9961 agccggcccc cgtatccatg caacccaagg cgaccagctc gccgcccgcc catagtgagg
    10021 ccgatatgtc acacatgctc aaggacatcg ctgtcttcta cgacgtcagc cacgaaggaa
    10081 agcacattct cgaaacggct gcggttctgg ccgaaaagca aaatgcccgg ctgatcggca
    10141 tcaccgccac gaatagttca gacatcgaga gctatccgtc ggacagcttt gcaagagggg
    10201 ccggcattgg ggaagtcatc agtcgcctgc agtcctcgac cgccgcgcat ctgctgcacg
    10261 cggcacaggc tttgaaagag gtggctgacc gtcacggcgt gacggccgag ttccgcgcaa
    10321 taccctggtc ggaatccggc gtcgagatga gcctgcactc gctctactgc gatctgctcg
    10381 tggtcaacca tccgaaggcg ccgggggcgc ctttcgcctg gtcgtccatc gacgtgcttc
    10441 aacaaaccgg cgtgccgctg ctgatcgtgc cccagtcctg gcgcagcatc cagttggggc
    10501 agcgcatcac cgtcgcctgg aatgccagtc ggcaggcccg ccgcgctgtc gccgatgcac
    10561 tgcctctgct gatttcggcc gaggcggtgg atctgttgat cgtggatcct gagcgcaagg
    10621 ccgagttgca tggagaggaa cccggcgcgg atatgggcag ctacctggcc cgccacggtg
    10681 tgcgggtgaa ccttgagcgg atcgactcgg cggacaagcc cgtcgccgat gtgatcctgg
    10741 agcacgcagt gaaggccaag gcggacttga tcgtcttcga tgcctacagc cgtccgaagc
    10801 tcagcgagct ggtgctgggc ggcgtcagcc ggaccttgtt gtccagcgtg cctctgccgc
    10861 tgttcgtttc tcactgaatg agccgttcgg catgagttaa ggttgagcaa tgggagcgat
    10921 ggtgcgaaga gggcctgcaa cgcgtgaaga catcctggat gctgcgcaat ggtgctacct
    10981 ccatctgggt gtgacaaacg cgagtacggc cgtgatcgcg gcgcgcgcga ggtgcccacg
    11041 gacagtggtc gtcagctact ttccagaacc gcaccaactg ttggacgagg tgctcaagcg
    11101 cggccgcctg tcgctggtgg acgaccttca gagcgttttt gatgcacaga tgcgtgtgat
    11161 gcctgctctg cgctcagcac tgcagttgtg tctcaacgag gtcgtccaca acgacagggt
    11221 tcgcgcagcg caggagatcc tggcgttcca ctgcgatttc ttcaccacgc aacgcaatct
    11281 tcttgaagag cagattgtcc atttcgcacg tgtcatgggc ttgctgcaga agatcgcgat
    11341 cgaagcggcc aagggcggtg aacttcgaga cggcgtacgc ccagatgcgt gggcattgat
    11401 catcggactg acattgagcg gagcgatccg cttatgcgtg atgcaaagga cggatgacgg
    11461 gcggctggcg tgctggagcg aggcaacgcg agcactcgac gaggctttct ctttcgtcgc
    11521 tccaggcccg cgacatcgtc tggcgccctc ccagagaaaa aacgggtaag gacatggctc
    11581 ggcggaaaaa ggaagactca ctcgaaacga gagaacggat cctcgatgcc gcggagtggt
    11641 gtttttgcac ctatggcgtc tctcaatcga ccttggcact cattgccgaa aaagcagaat
    11701 gcacccgggg agcaatctat tggcatttcc cgaaggaagc cgaggttctc aagcttgtgt
    11761 tggatcgagg gcgactgtcg ctgcagcggc ggcttgcctc gcttactcat gcacaaccac
    11821 ccctgatgcc caagctccaa gtctgtctcg acgactacct cgcagaggtc agccgaaaca
    11881 gacatgttcg tagcaccttg aagatcctcc tgctgcgatg cgactacgtt ggtctgcgtc
    11941 aggcgttcgt gggccggcgg tatgtggagg cactggaaat ctacggaccc ctcgtggacc
    12001 tgctcgctcg cgctcatgct gctggtgaga tggccccgga gctcgatccg cgcaacggag
    12061 cagaactgct gcaatgcctg ctgatcggag ggttgcgcaa gtggctgatc gactccatgg
    12121 acggggacga atccagcccg cgtgacatct tgagtctggc gttgggcttg ctccgagtgc
    12181 ggtagctatg ggggtgtgac ccacgcccct gatatgtcga tagcgcccag tcgaggctcg
    12241 acgcgctgtg cgcatgatat gcccctctgc tgatgctcgc atcagcgcgc tctggcaccg
    12301 tgcaacgcat gcttgccgcg cgtaatcttc ctactgtgac tccgtcggga ttgcccgccc
    12361 acgcagctgc gctggtcgct caggtgctcc agcgcctgcc gatgcagccc aacagtcgga
    12421 tcgaggactt gctgcctcac cgatggcggt ccgcatgcaa cgcgccatga cttccttcga
    12481 gatgatggtg ccatgggtcg cggccggcta cggcgtcggg gtgtcagcgc aatggcgcag
    12541 cgagcatggc catggatggg ggatcgccat gcgtccgctc gccgatggcc cctaagaaat
    12601 tgtggtgcat ctgcagcagc ctcacgggca gaccaacttt gtttcagagc ggttcgagcg
    12661 tagagcactg caagttgcca gggcaggtgc ggcctagcgg acgcttgcca tggccggggc
    12721 atcacacccc gaccatgggt tgaagcgtaa gggttaggcc ctctcaaata ccactgcgct
    12781 atccaccagc cgacggacgc tctgccgcac atcctcgggg ataggctgcg tggcaaccgt
    12841 ctcgggcaca tccgcgtggt gctggtagtc gcccatgatg acccggatga ccgcgggcac
    12901 gttcgtcggc gtgaccgcat caatggctgc gcgatcaccc aggtccgggt gcggctgggt
    12961 cagcatgcga tacaggtccc acgaatccgc gtgggggcac tggttcatca gcacctgggc
    13021 cagcttgtgc ttgagcggca ccagttgcca gtcgggaacc cgctgccctc ggttgcccag
    13081 gctgatggac agcagcttgc ccgccttcag ctcgcggttg atctgatcct tcgacttgcc
    13141 ggccagctta ccgaacagcg ggaccggcag attgttcggc gactcgtaga ccgccagcat
    13201 ttcctcgcgc tcgcgctgga tcgaggagac ttccggctcc ggggcatgcg tcggaggcgg
    13261 tggcacctgc gacagcctct ggaacgtaat ttcgccgctg tttggggtca cgacaatggg
    13321 cgtggctacc acgggcggca cgccagggac agcgggctcg gctaccgtgg gaaccgcgcc
    13381 caccgcttcc tccacttctg ccatcgctgg aaccccgtcg atacggatgg tcaagtgcgc
    13441 gctcgcggct tccacttcgc cctgttcgag caggtcgagg cgatcgcccc agtcctgcat
    13501 catccggcgg cgtggctcca cgtacttggc gtggttgtag gccgagctga ccttgttcgg
    13561 gtcggagtgc gaaagctgcg cgtccaccca aatcttcggg tagccgatct cgttgagtgc
    13621 cgttgagatc gtgccgcgga tgccgtggcc ggtcaggcgc ccctcatacc ccatgagctg
    13681 caaggccttg ttgagggtgt tctcgctgat gcgcttcttg agttcgctgc gatgcgacag
    13741 caggtacttc tgcgcgggcc gcatcacgcc caggagatag cggacgatct cgatggcctg
    13801 cagggacagg ggcacgatgt agggcggcac gtcctgcggc cgcttgccgg ccttgcgcat
    13861 ttcgtcctgg agctgcttga cgacctgcgg cgggatgatc cacaagccgc ggtcgaggtc
    13921 gaattgctca ggctcggcca gccgcagctc acccgtgcgc acccccgtca ggaacagcaa
    13981 ccggacgcca agctgggtct gccagccgcg ggggttgtag agccggagct tctgaaggaa
    14041 ctcgggcagc tcgggcaggt gcagataggg gttgtgggcc accggaggct taggctcggc
    14101 caccacgtcc aggtccgcgg ccgggttgac ttccagcccc tcggcgatga ccagggcata
    14161 gcggaacatc tggttgaacc aggtgcggac cttctcggcg gtggtgaacg ccttgcgctt
    14221 ctcgatcgcc gctacgacgc ccacgagctg gggccgacga atgtcgtaga tcgacatctt
    14281 tcccagggtg ggcagcacgt ccttgttgaa gatgcgcagg atctgcgaca gcgtgctctg
    14341 gcggccttcc ttgagttcct tgcggcgatg ctcaacccag gcatcgaaga cgttcttgaa
    14401 ggtgtattcg cccgccagcc tggccgcgtg ccggcgctgg tcgcgttcga tttggggatc
    14461 gatccccctg gcgagcaggg cttgagcctt gtcccgttcg gcacgagcct cacgcaggct
    14521 gagggcggga tagccgccca gggacatgcg tttctgcttg cccagccagt agtaacggaa
    14581 gatccacgac ttgccgcctg cggcagagac catcaggccc aggcagtcgt tgtcggagag
    14641 ggtgtagcgc ttgccggtgg tccttgcctg ccggactgtc agatcagaga gtgccatttc
    14701 gaggctccta agttatgact taggcctaat gctcgtcatg gttcccgctc agccccagca
    14761 actatccggt agccgtccca accgtattct tgtgcctcaa ttggtcactc aaaaacggta
    14821 gctgtgggtg gatttccgtg gcgctcgctg gaatgaaaaa aggggccgaa gccccttttt
    14881 tcaacgactt acagacgtca gtagaagtct gtagatcact atctggagcg ggataagaga
    14941 ctcgaactct cgacctcgac cttggcaagg tcgcgctcta ccaactgagc taatcccgcg
    15001 gatgacacgc aagtccggcg aaccgggctt cattgcaagt tgtggaggcc tgggtcggaa
    15061 t
//
LOCUS       DATCUN010000274         1924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_106933, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000274 DATCUN010000000
VERSION     DATCUN010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106933"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1272
                     /locus_tag="VIO83_10705"
     CDS             172..1272
                     /locus_tag="VIO83_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8331781.1"
                     /translation="MNQPVPDLSAAPDAAAVAPIAPRPRGPLSLVILALLACGYTLWA
                     AQAVILPVLLAGFLALVGNPLIRGLQKFHLPRFLGAALVLVLGLAAAATLTYQLVPPA
                     AAWAKAAPRELRQLAPKLRALTKPVHDANEAAENFARAAAGDTGRRPQIMRTASDPFR
                     TLTATPRMVASVLAVVLLTFFFMVYGASLQRHAIALLPRRQQQKLTVGILQSIERSVS
                     RYVLTISSINALVGLLFAGALFLLAFPLADALLWGTLVALLNFAPFVGPLMGMVMMLV
                     VGLTAFDDGFKSLLPAAIYLGLHTLEGQVVTPIILGRQMRLSPLILILALMVFGSLWG
                     IIGLLLAVPMLVCIKLILAQVQGMEGWARLME"
BASE COUNT          270 a          643 c          643 g          368 t
ORIGIN      
        1 accgcgcggg cggccgggat ggccaccgcc ggcgatggcg gccacggccc acacgggtca
       61 tcctatccgc gcgcttcgga agcgcgcggc gaaaccgaac cctgggcggc gcatccgcca
      121 cgcccggccg aggcagcaac cgacgtttcc gaacgctgag acgctgtttc catgaaccag
      181 ccggttcctg acctgtccgc cgcaccggac gccgctgcgg tcgcaccgat cgcgccacgt
      241 ccgcgcggac cgttgtcgct ggtgatcctg gccttgctgg cctgcggcta caccctgtgg
      301 gctgcgcagg cggtgatcct gccggtgctg ctggccggtt tcctggcgct ggtgggcaat
      361 ccgctgatcc gtggcctgca gaaattccac ctgccgcgct tcctgggcgc ggcgctggtc
      421 ctggtccttg gattggcggc cgccgccacg ctgacctacc agctggtgcc accggccgcg
      481 gcctgggcca aggccgcgcc gcgcgagctg cgccagctgg cgcccaagct gcgtgcgctg
      541 accaagccgg tgcatgacgc caacgaggct gcggaaaatt tcgcccgcgc cgccgccggc
      601 gacaccgggc gccggccgca gatcatgcgc accgccagcg atcccttccg gaccttgacc
      661 gccaccccgc gcatggtcgc ctcggtgctg gcggtggtgc tgctgacctt ctttttcatg
      721 gtctacggcg cctcgctgca gcggcacgcc atcgccctgc tgccgcggcg ccagcagcag
      781 aagctcacgg tcggcatcct gcagtcgatc gaacgctcgg tgtcacggta cgtgctgacc
      841 atcagcagca tcaatgcgct ggtggggctg ctgttcgccg gggcgctgtt cctgctggcc
      901 ttcccgctgg ccgacgcgct gctgtggggc acgctggtgg cgctgctgaa cttcgccccg
      961 ttcgtgggcc cgctgatggg catggtgatg atgctggtgg tgggcctgac cgccttcgat
     1021 gacggattca agtcgctgct gccggccgcc atctacctgg gcctgcacac cctcgaaggc
     1081 caggtggtga cgccgatcat cctgggccgg cagatgcggc tctcgccact gatcctgatc
     1141 ctggcgctga tggtgttcgg ctcgctgtgg gggatcatcg ggctgctgct ggcagtgccg
     1201 atgctggtgt gcatcaagct gatcctggcg caggtgcagg ggatggaggg ctgggcgcgg
     1261 ttgatggagt gatccatcgc tgtgcactcg cttcgcttgt gctgttgctg tgcttttgct
     1321 gtcgctctcg cttgcgctgt tgcagttgca cagttgctta cgctgttgca attgcggttg
     1381 cttgcgctgt tgcggttgca gtggcttttt gcctttgccc ctctgaccga gccgtaaagc
     1441 acgccgagca ccggagcctg atcgggccga agaggcgcgg gtgtctgagc gcagcgagtt
     1501 cccgcgccgt gcccgagcag gcgaggagcg caggggaccg atgcgcagca tcggcgtgcg
     1561 tccggcgaga agcggttttg gctacttttg ccgcgacaaa agtagctcgc gtcgcgaagc
     1621 gacgtgaaag ctcttgatcc tgcctgtgat ccattggtcc agccaagaaa atcttgaatc
     1681 aaaagcaagg ctttcgcgct tgcagcgcga cttccttttg tcttggcaaa agtagccaaa
     1741 accgctactc gccggacgcg atccgccgct gcgcggcggt cccctgcgct tctcggaagt
     1801 ccgggcacgg cgcccaaact cgctacgctc agacaagggc gcctcttcgg cccggacttc
     1861 ctgcgatgct cggctcgctt tacggctcag acaggaaagc gaaagcaaag ccagagccag
     1921 agcc
//
LOCUS       DATCUN010000275         6505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_87440, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000275 DATCUN010000000
VERSION     DATCUN010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87440"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1592
                     /locus_tag="VIO83_10710"
     CDS             72..1592
                     /locus_tag="VIO83_10710"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8331782.1"
                     /translation="MIYAQPGTAGAILSLKPRYGNFIGGKFVAPVKGQYFTNTSPVTG
                     EVIAEFPRSDAADIELALDAAHAAADAWGRTSVQDRSNLLLKIADRIEQNLELLAVAE
                     TWDNGKAVRETLNADVPLAVDHFRYYAGCIRAQEGSAAEINEHTAAYHFHEPLGVVGQ
                     IIPWNFPLLMAAWKLAPALAAGNCIVLKPAEQTPLSITVLMEVIGDLLPPGVLNVVQG
                     FGREAGEALATSKRIAKIAFTGSTPVGAHILKCAAENIIPSTVELGGKSPNIFFEDIM
                     RAEPEFIEKAAEGLVLAFFNQGEVCTCPSRALVQESIYDDFMAAVMKKIKQIKRGNPL
                     DTETMVGAQASDQQFEKILSYFDVARAEGAEILTGGAAEKLEGSLSSGYYIQPTLLKG
                     HNKMRVFQEEIFGPVVSVTTFKDEAEALAIANDTEFGLGAGVWTRDINRAYRMGRGIK
                     AGRVWTNCYHLYPAHAAFGGYKKSGVGRETHKMMLDHYQQTKNLLISYDVNPLGFF"
     gene            1895..1966
                     /gene="pqqA"
                     /locus_tag="VIO83_10715"
     CDS             1895..1966
                     /gene="pqqA"
                     /locus_tag="VIO83_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HEY8331783.1"
                     /translation="MWTKPAFTDLRIGFEVTMYFANR"
     gene            2014..2925
                     /gene="pqqB"
                     /locus_tag="VIO83_10720"
     CDS             2014..2925
                     /gene="pqqB"
                     /locus_tag="VIO83_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HEY8331784.1"
                     /translation="MHIRILGSAAGGGFPQWNCNCRNCRGLREGTLSATARTQSSIAL
                     SDDGVNWIICNASPDIRAQIEAFPALQPARALRDTAIRALILLDSQIDHTTGLLTLRE
                     GCPHEVWCSEMVHQDLTSGFPLFNMLEHWNGGLRWNPIDLEQSFTVAACPALRFTPIP
                     LRSAAPPYSPHRNDPHPGDNLGLLVEDSRTGGRLFYAPGLGQVDGELLEWMDRADCLL
                     VDGTLWRDDEMIHCGVGDKLGSAMGHLPQSGAGGMLEVLERLPRQRKVLIHINNTNPI
                     LDEDSPERAELGARGIEVAWDGMHIEL"
     gene            3035..3787
                     /gene="pqqC"
                     /locus_tag="VIO83_10725"
     CDS             3035..3787
                     /gene="pqqC"
                     /locus_tag="VIO83_10725"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEY8331785.1"
                     /translation="MTQTAMSPAEFEQALRAKGAYYHIHHPFHVAMYEGRASREQIQG
                     WVANRFYYQVCIPVKDAAIMANCPDRDTRREWIQRIVDHDGAPGEEGGIEAWLRLGEA
                     VGLTRAQLMSQELVLPGVRFAVDAYVNFARRACWQEAASSSLTELFAPHIHQSRLDAW
                     PAHYPWIDAGGYDYFRKRLKEARRDVEHGLRITLAHYTTFAAQQRMLEILQFKLDVLW
                     SMLDAMSMAYELQRPPYHTVTAERVWHRGIAL"
     gene            3903..4181
                     /gene="pqqD"
                     /locus_tag="VIO83_10730"
     CDS             3903..4181
                     /gene="pqqD"
                     /locus_tag="VIO83_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEY8331786.1"
                     /translation="MSDDIQLKIPALRRGFRFQWEPAQGCHVLLYPEGMIQLNDSAGA
                     ILAEIDGVRSVAAIIDDLSVRFPDVPGIDEDILAFLEVAHERFWIELR"
     gene            4153..5307
                     /gene="pqqE"
                     /locus_tag="VIO83_10735"
     CDS             4153..5307
                     /gene="pqqE"
                     /locus_tag="VIO83_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEY8331787.1"
                     /translation="MNGSGSSFAKPGHTPGPPLWLLAELTYRCPLQCPYCSNPLDFAK
                     QGEELSTAQWIEVFRQARELGAAQLGFSGGEPLVRQDLVELIAAARELGYYTNLITSG
                     IGLTEEKIASFREAGLDHIQVSFQAADEEVNNLLAGSPKAFAQKLAMARAVKAHGYPM
                     VLNFVTHRHNIDNIERIIELCLELEADFVELATCQFYGWAELNRAGLLPTRAQLERAE
                     RITNEWRDKLAAQKHPCKLIFVTPDYYEERPKACMNGWGNLFLDITPDGTALPCHSAR
                     QLPVQFPNVREHSLEHIWFESFGFNRYRGDDWMPEPCRSCDEKDRDFGGCRCQAFLLT
                     GNADNADPVCSKSAHHGLILDARREAEEAPLAVDELIYRNARASQIICKG"
     gene            5416..>6505
                     /locus_tag="VIO83_10740"
     CDS             5416..>6505
                     /locus_tag="VIO83_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY8331788.1"
                     /translation="MSTLRPALQSLPCGDWPSAWSAEQAAAASRDFAELRAGLGGLLW
                     LAFDPAEAGCSLWLWREGITRRLTPPGLSVRSRVYEYGGGAFCVAGDGVALVDEADQQ
                     VHLLDVAAGEWRALTARPDCRYGDLHFAPAWQAVLAVEESRAASAVVHRLVSLALADG
                     SRHVLAEGADFYSAPRLSGDGRWLVWIEWERPELPWTATRLCRAAVHADGSLGPREVL
                     AGVAGNESLQQPMFTADDRLTCLSDRAGWWQPWAEQGGRWRPVSSGGWVVENAGAVFH
                     GVAIHPTVGVGVGTGTEAGRRVDNGAALSTVSTADWSGPRPVARADHAPAPWQLGTVS
                     HLPLNGGGWLLSRLQEGWGLLVERDGAGR"
BASE COUNT         1059 a         2324 c         2127 g          995 t
ORIGIN      
        1 ggctgacacc atcaacccac agtccgcgca gaaccagatc ggaccgcaac aataaaagag
       61 aggccatgag catgatctac gcacaaccgg gtaccgctgg cgccatcctt tccctcaagc
      121 cgcgctacgg caacttcatc ggcggcaagt tcgtcgcgcc ggtcaagggc cagtacttca
      181 ccaacacctc gccggtcacc ggcgaagtga tcgccgaatt cccccgttcc gatgccgccg
      241 acatcgagct ggcgctggac gccgcccatg ccgccgccga cgcctggggc cgcacctcgg
      301 tgcaggatcg ctccaacctg ctgctgaaga tcgccgaccg catcgagcag aacctcgagc
      361 tgctcgccgt cgccgagacc tgggacaacg gcaaggccgt gcgcgagacg ctgaacgccg
      421 acgtgccgct ggccgtcgac cacttccgct actacgccgg ctgcatccgc gctcaggaag
      481 gcagcgccgc cgagatcaac gaacacaccg ccgcctacca ctttcacgag ccgctgggcg
      541 tggtcggcca gatcatcccg tggaacttcc cgctgctgat ggccgcctgg aagctggccc
      601 cggcgctggc cgccggcaac tgcatcgtgc tcaagccggc cgagcagacc ccgctgtcga
      661 tcaccgtgct gatggaagtc atcggcgacc tgctgccgcc gggcgtgctc aacgtggtac
      721 agggcttcgg tcgcgaggcc ggcgaggcgc tggccaccag caagcgcatc gccaagatcg
      781 ccttcaccgg ctccaccccg gtcggcgcgc acatcctcaa gtgcgccgcc gagaacatca
      841 tcccgagcac cgtcgagctg ggcggcaagt cgccgaacat cttcttcgaa gacatcatgc
      901 gcgccgagcc ggagttcatc gagaaggctg ccgaaggcct ggtgctggcc ttcttcaacc
      961 agggcgaggt gtgcacctgc ccgtcgcgcg cgctggttca ggagtcgatc tacgacgact
     1021 tcatggccgc ggtgatgaag aagatcaagc agatcaagcg cggcaacccg ctggataccg
     1081 agaccatggt gggtgcccag gcttccgacc agcagttcga gaagatcctc tcctacttcg
     1141 acgtagcccg cgccgaaggc gccgagatcc tcaccggcgg cgccgccgag aagctcgagg
     1201 gcagcctgtc cagcggctac tacatccagc cgaccctgct caagggtcac aacaagatgc
     1261 gcgtgttcca ggaggaaatc ttcggcccgg tggtcagcgt gaccaccttc aaggacgaag
     1321 ccgaagccct ggcgatcgcc aacgacaccg agttcggcct cggcgccggc gtgtggaccc
     1381 gcgacatcaa ccgtgcctac cgcatgggtc gcggcatcaa ggccggccgc gtgtggacca
     1441 actgctacca cctgtacccg gcgcacgccg ccttcggtgg ctacaagaag tccggcgtcg
     1501 gccgcgaaac ccacaagatg atgctcgacc actaccagca gaccaagaac ctgctgatca
     1561 gctacgacgt caatccgctc ggcttcttct gagttccctc tagggcgcgc cgctctcctg
     1621 gcgcgcccct gcttgtcgac gcgcctgctt cgggcgcgcg gtctgccggt ggttacccat
     1681 ggcccacggc ggacccttgt cgaacgcggc tccggccgcg ttttttttcg tccgcagttt
     1741 gcccggggcg catgccgcgg gccccggcgg ttggtcggcc agggctacca aggtcagcct
     1801 aaactccttc caaagtacca tttggcccgc gcgctggcgg gtgcataact gtcttgccgg
     1861 aattcaccgg tttcgacaac aagaggactg cgccatgtgg accaagcccg ccttcaccga
     1921 tctgcgcatt ggcttcgaag tgacgatgta cttcgccaac cgttgatcca catagccccg
     1981 gccctgccgg ggcttcccgc ccggtgtctg cccatgcata tccgtattct cggttccgcc
     2041 gccggcggtg gttttcccca gtggaactgc aactgccgca actgccgcgg cctgcgcgaa
     2101 ggcaccctga gtgccacggc gcgtacccaa tcctccatcg ccctgtccga tgacggggtc
     2161 aactggatca tctgcaacgc ctcgccggac atccgcgcgc agatcgaggc cttcccggcc
     2221 ctgcagccgg cccgcgcact gcgcgacacg gcgatccgcg cgctgatcct gctggacagc
     2281 cagatcgacc acaccaccgg cctgctcacc ctgcgcgaag gctgcccgca cgaggtgtgg
     2341 tgcagcgaga tggtccatca ggacctgacc agcggcttcc cgctgttcaa catgctcgag
     2401 cactggaacg gcggcctgcg ctggaatccc atcgatctcg agcagagctt caccgtcgcc
     2461 gcctgcccgg cgctgcgctt cacgccgatt cccctgcgca gcgcggcgcc gccctactcg
     2521 ccgcaccgca acgacccgca ccccggcgac aacctcggcc tgctggtcga ggacagtcgc
     2581 actggcgggc gcctgttcta cgcccccggc ctcggccagg tcgacggcga gctgctggag
     2641 tggatggacc gcgccgactg cctgctggtc gacggcaccc tgtggcgtga cgacgagatg
     2701 atccactgcg gcgtcggcga caagctcggc agcgccatgg gccacctgcc gcagagcggc
     2761 gctggcggca tgctcgaggt gctcgagcgc ctgccgcgcc agcgcaaggt gctcatccac
     2821 atcaacaaca ccaacccgat cctcgacgag gactcgcccg agcgcgccga gctgggcgcc
     2881 cgcggcatcg aggtcgcctg ggacggcatg cacatcgagc tgtaagccac gggtgatagt
     2941 gcgcatggcg caccctacgt cagccgtacc tgcctgtacg caccccacca gaacaacaac
     3001 gagccgcacc ggtcgcggcc gccgaggagt cgcgatgacc cagactgcca tgagccccgc
     3061 cgaattcgag caggcgctgc gcgccaaggg cgcctactac cacatccacc acccgttcca
     3121 cgtcgccatg tacgagggcc gcgcgagccg cgagcagatc cagggctggg tggccaaccg
     3181 cttctactac caggtgtgca tcccggtgaa ggacgcggcg atcatggcca actgcccgga
     3241 ccgcgacacc cgccgcgagt ggatccagcg catcgtcgac cacgacggcg cgcccggaga
     3301 ggagggcggc atcgaggcct ggctgcgcct gggcgaggcg gtcggcctga cccgcgcgca
     3361 gttgatgtcg caggaactgg tgctgcccgg cgtgcgcttc gccgtggacg cctacgtcaa
     3421 cttcgcccgc cgcgcctgct ggcaggaagc ggccagcagc tcgctgaccg aactgttcgc
     3481 cccgcacatc caccagtcgc gcctcgacgc ctggccggcg cactacccgt ggatcgacgc
     3541 cggcggctac gactacttcc gcaagcgcct caaggaggcg cgccgcgacg tcgagcacgg
     3601 tctgcgcatc actctcgcgc actacaccac cttcgcggcc cagcagcgca tgctggagat
     3661 cctgcagttc aagctcgacg tgctgtggag catgctcgac gccatgagca tggcctacga
     3721 gctgcagcgc ccgccgtacc acacggtgac cgccgagcgg gtctggcacc gggggatcgc
     3781 gctgtgagcc cgaatgtagg gtggatggcc accccgtgga aaacgaccgc aggtcttttc
     3841 caccgtcgcg gtcagtggtg ggaaaacgct gcgcgtgttt cccaccctac ggaggtgcgc
     3901 ccatgagcga cgacatccag ctgaagatcc cggcgctgcg ccgcggtttc cgcttccagt
     3961 gggaaccggc gcagggctgc cacgtgctgc tctatcccga gggcatgatc cagctcaacg
     4021 acagcgccgg ggcgatcctc gccgagatcg acggcgtgcg cagcgtcgct gccatcatcg
     4081 atgacctcag cgtgcgcttc ccggatgtcc cgggcatcga cgaggacatc ctggctttcc
     4141 tggaggtggc ccatgaacgg ttctggatcg agcttcgcta agccggggca cacgcccggc
     4201 ccgccgctgt ggctgctggc cgagttgacc tatcgctgcc cgctgcagtg cccgtactgc
     4261 tccaacccgc tggacttcgc caagcagggc gaggagctga gcaccgcgca gtggatcgag
     4321 gtgttccgcc aggcccgcga gctgggcgcc gcccagctcg gcttctccgg tggcgagccg
     4381 ctggtgcgcc aggacctggt cgagctgatc gccgccgccc gcgagctggg ctactacacc
     4441 aacctgatca cttccggcat cggcctgacc gaggagaaga tcgccagctt ccgcgaggct
     4501 ggcctcgacc acatccaggt cagtttccag gccgccgacg aggaggtgaa caacctgctg
     4561 gccggctcgc ccaaggcctt cgcccagaag ctggcgatgg cccgcgcggt caaggcccac
     4621 ggctacccga tggtgctcaa cttcgtcacc catcggcaca acatcgacaa catcgagcgg
     4681 atcatcgagc tgtgcctcga gctggaggcc gacttcgtcg agctggccac ctgccagttc
     4741 tacggctggg ccgagctgaa tcgcgccggc ctgctgccga cccgcgccca gctggagcgc
     4801 gccgagcgca tcaccaatga gtggcgcgac aagctcgccg cgcagaagca tccgtgcaag
     4861 ctgattttcg tcacccccga ctactacgag gagcgcccca aggcgtgcat gaacggctgg
     4921 ggcaacctgt tcctcgacat caccccggac ggcaccgcgc tgccctgcca cagcgcgcgc
     4981 cagctgccgg tgcagttccc caacgtgcgc gagcacagcc tcgagcacat ctggttcgag
     5041 tcgttcggtt tcaaccgcta ccgcggcgac gactggatgc ccgagccgtg ccgctcctgc
     5101 gacgagaagg accgcgactt cggcggctgc cgttgccagg cgttcctgct caccggcaat
     5161 gccgacaacg ccgacccggt gtgcagcaag tcggcgcacc acggcctgat cctcgacgcc
     5221 cgccgcgagg ccgaggaggc gccgctcgcc gtcgacgagc tgatctaccg caatgcgcga
     5281 gcctcgcaga tcatctgcaa gggctgaggg gagattgcat gggcggggca cggtgtaggg
     5341 tggatggcca ccccgtggaa aaccgcgtca gcgttttcca gcgcagaggc cgctgcggtg
     5401 gataaggctg cgccgttgtc caccctacgt cctgcgctcc agtcgctgcc ctgcggcgac
     5461 tggcccagcg cctggtcggc cgagcaggcc gccgcggcca gccgcgattt cgccgagctg
     5521 cgcgccggcc tcggcggcct gctgtggctg gccttcgacc cggccgaggc gggttgcagc
     5581 ctgtggttgt ggcgcgaggg cattacccgg cgcctgacgc cgccggggct gtcggtgcgc
     5641 agccgggtct acgaatacgg cggcggtgcc ttctgtgtgg cgggcgacgg cgtggcgctg
     5701 gtcgacgagg cggaccagca ggtccatctg ctggacgtgg ccgctggcga gtggcgcgcg
     5761 ctgaccgcac gtcccgactg ccgctacggc gacctgcact tcgcgccggc gtggcaggcg
     5821 gtgctggcgg tggaggaaag ccgcgcggcg tcggcggtgg tgcaccgact ggtcagcctg
     5881 gcgctggccg acggcagccg ccacgtattg gccgagggcg ccgatttcta cagcgcgccg
     5941 cgcctgagcg gcgacggccg ctggctggtg tggatcgagt gggagcgtcc cgagctgccg
     6001 tggactgcca cccggctgtg ccgcgccgcg gtgcatgccg atggctcgct cggtccgcgt
     6061 gaagtgctgg ccggcgtggc gggcaatgaa tcattgcagc aaccgatgtt cactgccgat
     6121 gaccggctga cctgcctgag cgaccgcgcc ggctggtggc agccgtgggc ggagcagggc
     6181 ggacgatggc gtccggtttc ttcaggtgga tgggtggtgg aaaacgctgg cgcggttttc
     6241 cacggggtgg ccatccaccc tacggtcgga gtcggagtcg ggaccgggac cgaggcgggg
     6301 cgtagggtgg acaacggcgc agccttgtcc accgtttcca ccgcggactg gagcggaccc
     6361 cggcccgtgg cacgcgccga ccacgccccg gcgccctggc agctgggcac cgtcagccac
     6421 ctgccgctga acggtggcgg ctggctgctc agccgcctgc aggagggctg ggggctgctg
     6481 gtcgagcgcg acggcgccgg ccgcg
//
LOCUS       DATCUN010000276         7163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_107158, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000276 DATCUN010000000
VERSION     DATCUN010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107158"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..491
                     /gene="hemJ"
                     /locus_tag="VIO83_10745"
     CDS             63..491
                     /gene="hemJ"
                     /locus_tag="VIO83_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HEY8331789.1"
                     /translation="MLYLWLKALHIIAIVCWFAGLFYLPRLFVYHAMSEDAASRERFC
                     IMERKLYRGIMGPSMIATLVFGIWMLVANPALFSSGAWLHAKLTLVVLLIGYHHGCGA
                     LLKRFARGDNQRSHVFYRWFNEVPVLFLLAIVILVVVKPF"
     gene            581..1369
                     /locus_tag="VIO83_10750"
     CDS             581..1369
                     /locus_tag="VIO83_10750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8331790.1"
                     /translation="MPQYALITGASSGIGLALAEALARRGHALILVARERPALESIAC
                     ELSQRFGVEVMFRVCDLTEPLQLSGLLYEVEQCDYQIELLINNAGTACAGSFLDNDWQ
                     RERKLLELNVFALARLCHALGNAMARRGSGRILNVASLAAFQPGPWLSSYYASKAFVL
                     HFSEGLREELQGQGVQVSALCPGPVRTPFWRNARLDMRPLLGSKWLMSAEQLAQLALL
                     GLDRQQAVIVPGWRNRLLARAAQLSPRRLARRLAARLNRHLLPR"
     gene            complement(1377..2858)
                     /locus_tag="VIO83_10755"
     CDS             complement(1377..2858)
                     /locus_tag="VIO83_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8331791.1"
                     /translation="MKNDIHDLGLVLDSRVRLIVVESWDEQRVLETLSALAIRRGLGL
                     YLWSVTEGVQRLGFGGEPLGEGDSREPEVALRLVKADPQANLYVFCDLHPFLADNPRL
                     VRLLKEVAMASGPQRATLVLVSHALKLPAELQRLAARFSLALPGEDELLAIVREEAAR
                     WSERNHGVRVRTDNRTLQQLVKNLRGLAHAEARLLARNVICNDGAITQDDLPELNRAR
                     FQLLDMDGVLSFEYETARFAEVGGLNALKRWLGERREAFLAADASDLPRGVLLVGVQG
                     GGKSLAAKAVAGLWGLPLLRLDFACLYNKYFGETERNLREALKMAEQMAPCVLWLDEI
                     EKGLASGDHDGGVSQRVLGTLLTWMAERQAPVFMVATANAIHRLPPELVRKGRFDELF
                     FVDLPDAEVRAEIFRIHLARRELEPGQFDLALLAAASDGFSGAEIEQAVVSALYAAQA
                     RQCAVDQLLLLQALQQTAPLSVVMAEELAALRAWAAERTVRAD"
     gene            2942..3280
                     /locus_tag="VIO83_10760"
     CDS             2942..3280
                     /locus_tag="VIO83_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEY8331792.1"
                     /translation="MDCLFCKIVDGAIPARKLYEDDQVVAFHDIGPQAPVHFLVIPKK
                     HIATLHDIGEEDKALVGHIVHTAQRLAREQGCEEGFRVVMNCNEQGGQTVYHIHMHVL
                     GQRQMHWPPG"
     gene            3365..4012
                     /gene="coq7"
                     /locus_tag="VIO83_10765"
     CDS             3365..4012
                     /gene="coq7"
                     /locus_tag="VIO83_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEY8331793.1"
                     /translation="MTSERHYSPVDRLLLQADAALRTLLPFSGQPTRPSPATPREEVA
                     LDEAEARHVCGLMRINHTGEVCAQALYQGQALTAKLPQVRQAMEQAADEEIDHLAWCE
                     QRIRELGGRPSLLNPLFYGLSFGIGASAGLISDRVSLGFVAATEDQVVKHLDEHLAQL
                     PPGDQRSQAILEQMREDELQHATAALDAGGLRFPAPVKAGMSLLSKVMTRATYRL"
     gene            complement(4078..4872)
                     /gene="speD"
                     /locus_tag="VIO83_10770"
     CDS             complement(4078..4872)
                     /gene="speD"
                     /locus_tag="VIO83_10770"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEY8331794.1"
                     /translation="MKSKLKLHGFNNLTKTLSFNIYDICYAETPEDQQAYVQYIDEVY
                     DAERLTQILTNVVEIIGANILNIARQDYDPQGASVTILISEQPVEPTDSQIEESPGPL
                     KETILAHLDKSHITVHTYPEIHPVEGIATFRVDIDVSTCGVISPLKALNYLIHSFDSD
                     IVTVDYRVRGFTRDVEGKKHFIDHEINSIQKYLSADTKAAYQMTDVNVYQENLFHTKM
                     LLKDFDLDNYLFGDATNNLSAGQREQVSERVKHEMLEIFYGRNMPR"
     gene            complement(5119..5541)
                     /locus_tag="VIO83_10775"
     CDS             complement(5119..5541)
                     /locus_tag="VIO83_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY8331795.1"
                     /translation="MKARIQWAGEAMFLGESGSGHVVVMDGPPENGGRNLGVRPMEML
                     LLGLGGCSSFDVVSILKKSRQAVESCEAFLEAERAGEDPKVFTKIHLRFVVKGRGLKE
                     AQVKRAVELSAEKYCSASIMLARGGVEISHGYEIVELG"
     gene            5770..6414
                     /gene="crp"
                     /locus_tag="VIO83_10780"
     CDS             5770..6414
                     /gene="crp"
                     /locus_tag="VIO83_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HEY8331796.1"
                     /translation="MVAISLTPKIKNLDKLLAHCHRRRYTAKSTIIYAGDRCETLFFI
                     IKGSVTILIEDDDGREMIIAYLNPGDFFGEMGLFDLEGREQERSAWVRAKTECEVAEI
                     SYAKFRELTQQDPDILYTLGSQMADRLRKTTRKVGDLAFLDVTGRVARTLLELCKQPD
                     AMTHPDGMQIKITRQEIGRIVGCSREMVGRVLKALEEQGLVNVKGKTMVVFGTR"
     gene            complement(6476..>7163)
                     /gene="trpC"
                     /locus_tag="VIO83_10785"
     CDS             complement(6476..>7163)
                     /gene="trpC"
                     /locus_tag="VIO83_10785"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016982714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY8331797.1"
                     /translation="PRGFARALQAQAAQRRPAVIAEVKKASPSKGVIRDPFLPAEIAA
                     SYAAGGATCLSVLTDVDFFQGSNAYLQQARAACNLPVIRKDFLIDPYQVVEARAIGAD
                     CVLLIVSCLDDGRLVELAQVAREQNLDVLVEVHDGAELERALKHLDTPLLGINNRNLH
                     SFEVSLQTTLDLLPRIPQDRLVVTESGILARADVELMERHDVWSFLVGEAFMRAEEPG
                     EELRRLFFPA"
BASE COUNT         1204 a         2529 c         2237 g         1193 t
ORIGIN      
        1 tgacgccgtg cccggcctgc gactaccatc tgcgcagacc ctaatcgaga aggacatccc
       61 gcatgctcta cctgtggctc aaggccctgc acatcatcgc catcgtctgc tggttcgccg
      121 gcctgttcta cctgccgcgc ctgttcgtct accacgcgat gagcgaagac gccgccagcc
      181 gcgagcgctt ctgcatcatg gaacgcaaac tgtaccgcgg catcatgggc ccctcgatga
      241 tcgccaccct ggtgttcggt atctggatgc tggtcgccaa ccccgccctg ttcagctccg
      301 gcgcctggct gcacgccaaa ctgaccctgg tcgtcctgct gatcggctac caccacgggt
      361 gcggcgccct gctcaagcgc ttcgcccgcg gcgacaacca gcgcagccac gtgttctatc
      421 gctggttcaa cgaggtgccg gtgctgttcc tgctcgccat cgtcatcctg gtggtggtca
      481 agcccttctg aagcgctgaa tcgggcagcc agcgccggcc gagcggagta cactgcgcgc
      541 tttttgcgcg gccccgcccg gcacgtctag gagaccccgc atgcctcaat atgccctgat
      601 caccggcgct tccagcggca tcggcctggc cctggccgaa gccctggcgc ggcgcggaca
      661 cgccctgatc ctggttgccc gcgaacgccc ggcgctggaa agcatcgcct gcgagctgag
      721 ccagcgcttc ggcgtcgaag tgatgttccg cgtctgcgac ctgaccgagc cgctgcaact
      781 gtccggtctg ctctatgaag tggagcagtg cgactaccag atcgaactgc tgatcaacaa
      841 cgccggcacc gcctgcgccg gcagcttcct cgacaacgac tggcagcgcg aacgcaagct
      901 gttggagctc aacgtgttcg ccctggcgcg cctgtgccac gccctcggca acgccatggc
      961 ccgccgcggc agcgggcgca tcctcaatgt cgcctcgctg gccgccttcc agcccgggcc
     1021 atggctgagc agctactacg ccagcaaggc cttcgtcctg catttctccg aaggcctgcg
     1081 cgaagaactg cagggacagg gcgtgcaggt ttcggcgctc tgccccggcc cggtgcgcac
     1141 gccgttctgg cgcaacgcac gcctcgacat gcgcccgctg ctcggcagca aatggctgat
     1201 gagcgccgag caactggccc agctggccct gctcggactc gatcgccagc aggcggtgat
     1261 cgtccccggc tggcgcaacc gcctgctggc ccgtgccgcc caactgagcc cgcgccgact
     1321 ggcccgtcgt ctggctgccc gcctcaaccg ccacctgctg ccacgctgag ccctgctcag
     1381 tcggcacgca ccgtgcgctc cgccgcccag gcacgcagcg ccgccagctc ctcggccatc
     1441 accaccgaca gcggcgcggt ctgctgcagc gcctgcagca gcaggagctg atccaccgcg
     1501 cactgccgcg cctgcgccgc atacagcgca ctgaccaccg cctgctcgat ctccgccccg
     1561 gaaaagccgt cgctggcggc cgccagcagc gccaggtcga attgcccggg ctccagctcg
     1621 cggcgagcca agtggatgcg gaagatctcc gcacgcacct ccgcgtccgg caaatcgacg
     1681 aagaacagct cgtcgaagcg ccccttgcgc accagctccg ggggcaggcg gtggatggca
     1741 ttggccgtcg ccaccatgaa caccggcgcc tggcgctcgg ccatccaggt cagcagggtg
     1801 ccgagcacgc gctggctcac cccgccgtca tggtcgccac tggccaggcc cttctcgatc
     1861 tcgtccagcc acagcacgca gggcgccatc tgctcggcca tcttcagcgc ctcgcgcagg
     1921 ttgcgctcgg tctcgccgaa gtacttgttg tacaggcagg caaaatccag gcgcagcagc
     1981 ggcagccccc acagtccggc caccgccttg gccgccaggc tcttgccgcc gccctgcacg
     2041 ccgaccagca gcaccccgcg cggcaagtcg ctggcatccg cggcgaggaa cgcctcgcgg
     2101 cgctcgccca gccagcgctt gagcgcattc agcccgccaa cctcggcaaa gcgcgccgtc
     2161 tcgtactcga agctgagcac gccatccatg tccagcaact ggaagcgcgc gcgattgagt
     2221 tccggcagat cgtcctgggt gatcgcgcca tcgttgcaga tcacgttgcg cgccagcaga
     2281 cgcgcctcgg catgggccag accgcgcagg ttcttcacca gctgctgcag ggtgcgattg
     2341 tcggtgcgta cccgcacgcc atggttgcgc tcgctccagc gcgccgcctc ctcgcggacg
     2401 atggccagca gctcgtcctc gccgggcagc gccaggctga aacgcgcggc caggcgctgc
     2461 agctcggccg gcagcttgag cgcatgggac accagcacca gggtcgcccg ctgtggaccg
     2521 ctggccatcg ccacttcctt gagcaggcgc accagccgcg gattgtcggc gaggaacgga
     2581 tgcaggtcgc agaacacgta gagattggcc tgtggatcgg ccttgaccag gcgcagcgcc
     2641 acctccggct cgcggctgtc gccctcgccc agcggctcgc cgccaaagcc cagacgctgc
     2701 acgccctcgg tcaccgacca caggtacagc cccagcccgc gcctgatcgc cagcgcactg
     2761 agggtttcca gcacccgctg ctcgtcccac gactcgacga cgatcaggcg cacgcgcgag
     2821 tcgagcacca ggcccagatc atgaatatcg ttcttcacac agactccttg tgggggtggg
     2881 cgacagcggg tggcgccgct acactcgggc gacccgcatc agcaactctg gagatacgct
     2941 tgtggactgc ctgttctgca agatcgtcga cggcgccatt ccggcgcgca agctgtacga
     3001 ggacgaccag gtggtcgcct tccacgacat cggcccgcag gcgccggtgc attttctcgt
     3061 gatcccgaaa aaacacattg ccacccttca cgatatcggc gaagaggaca aggccctggt
     3121 cggccacatc gtccataccg cccagcgcct ggcccgcgag cagggctgcg aggaaggctt
     3181 ccgcgtggtg atgaactgca acgagcaggg tgggcagacc gtctaccaca tccacatgca
     3241 cgtgctcggc cagcgccaga tgcactggcc gccgggctga tcgcgaccgc ctgctgatcc
     3301 cgctcaagtc cggcgccggc cgtttgcgcc agacttgccg ccaccgcctt tccggagatc
     3361 gccgatgacc agcgaacgcc actactcccc ggtcgatcgc ctgctgctgc aggccgatgc
     3421 cgccctgcgc accctgctgc cgttcagcgg ccagccaacg cgcccgtcac ccgcgacgcc
     3481 ccgcgaggag gtcgccctcg acgaggccga ggcgcggcat gtctgcggcc tgatgcgcat
     3541 caaccatacc ggcgaggtct gcgcccaggc gctgtaccag ggccaggcgc tgaccgccaa
     3601 gctgccgcag gtgcgccagg ccatggagca ggccgccgac gaggagatcg accacctggc
     3661 ctggtgcgag cagcgcatcc gcgagctggg cgggcgtccc agcctgctca acccgctgtt
     3721 ctacggcctg tcgttcggca tcggcgccag cgccgggctg atcagcgacc gggtcagcct
     3781 gggcttcgtc gccgccaccg aggatcaggt ggtcaagcac ctcgacgaac acctcgccca
     3841 gctgccgccc ggcgaccagc gctcgcaggc gatcctcgag cagatgcgcg aggacgaact
     3901 gcagcacgcc accgccgccc tggacgctgg cggcctgcgc ttcccggcgc cggtcaaggc
     3961 cggcatgagc ctgctgtcca aggtgatgac ccgggccacc taccgcctct gagccagcgg
     4021 cgccaggcac aaaaaagggc atcccgtggg atgccctttt gcgtagtgcc caaaatctca
     4081 gcgcggcatg ttgcggccgt agaagatctc cagcatctcg tgcttgaccc gctcgctgac
     4141 ctgctcgcgc tgcccggcgg agaggttgtt ggtggcatcg ccgaacaggt agttgtccag
     4201 gtcgaagtcc ttgagcagca tcttggtgtg gaacaggttc tcctggtaca cgttgacgtc
     4261 ggtcatctgg tacgccgcct tggtatcggc ggagaggtac ttctggatcg agttgatctc
     4321 gtgatcgatg aagtgcttct tgccttccac atcgcgggtg aagccacgca cgcggtagtc
     4381 gacggtgacg atgtccgaat cgaacgagtg gatcaggtag ttgagcgcct tcagcggcga
     4441 gatcaccccg caggtcgaca cgtcgatgtc gacgcggaag gtggcgatgc cttccaccgg
     4501 atggatttcc gggtaggtgt gcaccgtgat gtggctcttg tccaggtgcg cgaggatggt
     4561 ttccttcagc ggaccgggcg actcctcgat ctggctgtcg gtgggttcca ccggctgctc
     4621 ggagatcagg atggtcacgc tggcgccctg gggatcgtag tcctggcggg cgatgttgag
     4681 gatgttggcg ccgatgatct cgaccacatt ggtgagaatc tgcgtcaggc gctcggcgtc
     4741 gtagacctcg tcgatgtact ggacgtaggc ctgctggtct tccggcgtct cggcgtagca
     4801 gatgtcgtaa atattgaagc tcaaggtctt ggtcaggttg ttgaaaccgt gcagcttgag
     4861 tttgcttttc accgtcgatc actccccagg tggtgcggcg cggccgcggt tgtggcatgc
     4921 ccgtcagatg cttgcagcac ctgcgtggga cggcttgcac ctctgcgcgc tggcgactgg
     4981 ccatagaaga aggcgccgca ccatccgtgg atcgcgcggc gcccgaaaat aaagcccggc
     5041 attatgccga cggcggccac gctcgcctag agcctgccgg cagtttgtga ccgggcagtg
     5101 accccgcccg gcagcctgtc agcccagctc gacaatctcg tagccgtggc tgatctcgac
     5161 cccgccgcgg gccagcatga tcgacgccga gcagtacttc tcggccgaca gctccaccgc
     5221 gcgcttgacc tgcgcctcct tgaggccgcg gcccttgacc acgaagcgca ggtggatctt
     5281 ggtgaacacc ttggggtcct cgccagcgcg ctcggcctcg aggaaggcct cgcagctctc
     5341 caccgcctgg cgcgactttt tcaggatgct gaccacgtcg aagctgctgc agccgcccag
     5401 gccgagcagc agcatctcca tcggccgcac gccgagatta cgcccgccat tctccggcgg
     5461 cccatccatc accaccacat gcccgctgcc cgactcgcca aggaacatgg cctcgccggc
     5521 ccactggatg cgtgctttca tcaacctgac tccgcgctgg tgtataaagg ggaacgaaag
     5581 cttaacacag ccggccaagg ccgctcttta gtcgaacctc tgtgatctgc aacgcaattt
     5641 cgccctgccg aaggcctcac tactgttaag ctgacgccac tttgctggca cactgcagca
     5701 ccttttaata acaataaaaa catcgcctgc ggcacagggc tttttccttt ttactttccg
     5761 ggatcgggca tggttgctat tagcctcaca ccaaagatca agaacctcga caagctgctc
     5821 gcgcactgtc atcgccgtcg ctacaccgcc aagagcacca tcatctatgc cggtgatcgc
     5881 tgtgagaccc tgttcttcat catcaagggc tcggtgacca tcctcatcga ggacgacgac
     5941 ggccgcgaga tgatcatcgc ctacctgaat cccggcgact tcttcggcga gatggggctg
     6001 ttcgatctgg aaggccgcga gcaggagcgc agcgcctggg tccgagccaa gaccgagtgc
     6061 gaagtcgcgg agatcagcta tgccaagttc cgcgagctga cccagcagga cccggacatc
     6121 ctctacaccc tcggcagcca gatggccgac cgcctgcgca agaccacccg caaggtcggc
     6181 gacctggcct tcctcgacgt caccggccgc gtggcgcgca ccctgctcga gctgtgcaag
     6241 cagccggacg ccatgaccca ccccgacggc atgcagatca agatcacccg ccaggagatc
     6301 ggccgcatcg tcggctgctc gcgcgagatg gtcggccgcg tgctcaaggc cctggaggaa
     6361 caggggctgg tcaacgtcaa gggcaagacc atggtggtgt tcggcactcg ctgagattgt
     6421 cgaagcaaca aaaaggccgt ccctcgggac ggccttttgc gttctgcacc acggatcagg
     6481 cggggaagaa cagccggcgc agctcctcac cgggctcctc ggcgcgcatg aacgcctcgc
     6541 cgaccaggaa gctccatacg tcgtggcgct ccatcagctc gacgtcggcg cgggcgagga
     6601 tgccgctctc ggtgaccacc aggcgatcct gtggaatgcg cggcagcagg tcgagggtgg
     6661 tctgcagcga gacctcgaag ctgtgcaggt tgcggttgtt gatgccgagc agcggggtgt
     6721 ccaggtgctt gagcgcgcgc tcgagctcgg cgccgtcgtg cacctcgacc agcacgtcca
     6781 gattctgctc cctggccacc tgggccagct cgaccagccg accgtcgtcc aggcaagaca
     6841 caatcagcag cacgcagtcg gcgccgatgg cgcgcgcctc gaccacctgg tagggatcga
     6901 tgaggaagtc cttgcggatc accggcagat tgcaggcggc gcgcgcctgc tgcaggtagg
     6961 cgttgctgcc ctggaagaag tcgacgtcgg tgagcaccga cagacaggtg gcgccgccgg
     7021 ccgcgtagct ggcggcgatc tcggccggca ggaagggatc gcggatcacc cccttgctcg
     7081 gcgaggcctt cttcacctcg gcgatcaccg caggtcggcg ctgcgccgcc tgggcctgca
     7141 gggcgcgggc gaagccgcgc ggg
//
LOCUS       DATCUN010000277         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_201328, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000277 DATCUN010000000
VERSION     DATCUN010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201328"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VIO83_10790"
     CDS             complement(<1..371)
                     /locus_tag="VIO83_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015275110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HEY8331798.1"
                     /translation="MIGLCLGLAGTVWASLPVPAFTLAWQHTIEKVRWEEDYRVTAEG
                     LLLGEARVKGSGAGMEIPADAVLHDGSWHYRRPLPPLQPLRVGRTPEAGDYQLCFDGA
                     CHALSEWLGPPSAARPALELW"
     gene            complement(368..2392)
                     /locus_tag="VIO83_10795"
     CDS             complement(368..2392)
                     /locus_tag="VIO83_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEY8331799.1"
                     /translation="MSESQGMHASPSEWPKALFAVALLFSIYQIITAAFHPVTSQILR
                     AGHVGFLLLLVFLCYPAWGSKRPFQPLAWLLGLAGMSTFAYQWVFEAELIQRSGELTD
                     TDMVIGLLLIALVFEAARRVMGVALPLICGLFLAYGLFGEYLPGELAHRGYGLDQIVN
                     QLSFGTEGLYGTPTYVSATYIFLFILFGSFLEQAGMIKLFTDFSMGLFGHKLGGPAKV
                     SVASSALMGTITGSGVANVVTTGQFTIPLMKRFGYRPAFAGAVEATASMGSQIMPPVM
                     GAVAFIMAETINLPYVEVAKAALLPALLYFGSTFWMVHLEAKRAGLKGLPKEQCPNAW
                     GAVKERWYLLIPLAVLVYMLFSGRTPMFSGTVGLALTAIVILGSAIILKVSSSALRIA
                     FWVALGILCAGFFSLGIGVVFGVIALLVVVCWFIKGGRDTLVVCLHALVEGARHAVPV
                     GIACALVGVIIGVVSLTGVASTFAGYILAIGQDNLFLSLVLTMLTCLVLGMGIPTIPN
                     YIITSSIAAPALLDLGVPLIVSHMFVFYFGIMADLTPPVALAAFAAAPIARESGLKIS
                     LWAVRIAVAGFVVPFMAVYDPALMLQSDSWTAILYVLCKAAFAIALWGAVFTGHLQRD
                     LAWWERLLAFAAGVSLVMAMPVTDELGFALGALFLVQHIWRARRAEVAAA"
BASE COUNT          466 a          850 c          908 g          307 t
ORIGIN      
        1 ctccacagct ccagcgccgg ccgcgccgcg ctgggcgggc cgagccattc gctcagtgcg
       61 tgacaggcac cgtcgaaaca cagctggtag tcccccgcct cgggcgtgcg cccgacgcgc
      121 agcggttgca gcggtggcaa cgggcggcgg tagtgccagc tgccgtcgtg cagcacggcg
      181 tcggccggaa tctccatgcc ggcgccgctg cccttgaccc gcgcctcgcc gagcagcagg
      241 ccctcggcgg tgacgcggta gtcctcttcc cagcgcacct tctcgatggt gtgctgccag
      301 gccagggtga aggccggcac cggcaggctt gcccataccg tgccggccag ccccaggcac
      361 aggccgatca cgccgccgcc acctcggcgc ggcgggcgcg ccagatgtgc tggacgagga
      421 acagcgcgcc gagggcgaag ccgagctcgt cggtcaccgg catggccatg accagcgaca
      481 cgccggcggc gaaggccagc aggcgctccc accaggccag gtcacgctgc aggtggccgg
      541 tgaacaccgc gccccacagg gcgatggcaa aggccgcctt gcacagcacg tagaggattg
      601 ccgtccagct gtcgctctgc agcatcagcg ccgggtcgta caccgccatg aacggcacca
      661 cgaagccggc caccgcgatg cgcaccgccc acaggctgat cttcagcccg ctctcgcggg
      721 cgatcggcgc cgcggcgaag gcggccagcg ccaccggcgg ggtgaggtcg gccatgatgc
      781 cgaagtagaa gacgaacatg tgcgagacga tcagcggcac gccgaggtcg agcagtgccg
      841 gcgcggcgat cgagctggtg atgatgtagt tggggatggt cgggatgccc atgcccagca
      901 ccaggcaggt gagcatggtc agcaccaggg agaggaacag gttgtcctgg ccgatggcga
      961 ggatgtagcc ggcgaaggtg gaggccaccc cggtcagcga caccacaccg atgatcaccc
     1021 cgaccagggc gcaggcgatg cccaccggca ccgcgtggcg cgcgccctcg accagcgcgt
     1081 gcaggcacac gacgagggtg tcgcgcccgc ccttgatgaa ccagcagacc accaccagca
     1141 gggcgatcac gccgaacacc acgccgatgc cgagactgaa gaagccggcg cagaggatgc
     1201 cgagggccac ccagaaggcg atgcgcaagg cgctggacga caccttgagg atgatcgccg
     1261 agccgaggat gacgatggcg gtcagcgcca ggccgacggt gccggagaac atcggcgtgc
     1321 gcccggagaa cagcatgtag accagcacgg ccagcgggat cagcaggtac cagcgctcct
     1381 tgaccgcacc ccaggcgttc gggcactgct ccttgggcag gcccttgagc ccggcgcgct
     1441 tggcctccag gtggaccatc cagaaggtcg agccgaagta cagcagcgcc ggcagcaggg
     1501 cggccttggc cacctccacg tagggcaggt tgatggtctc ggccatgatg aaggccaccg
     1561 cgcccatcac cggcggcatg atctggctgc ccatgctggc ggtcgcctcc accgcgccgg
     1621 cgaaggccgg gcgatagccg aagcgcttca tcagcgggat ggtgaactgg ccggtggtca
     1681 ccacgttggc cacgccggag ccggtgatgg tgcccatcag cgccgaggag gccaccgaca
     1741 ccttggccgg gccgccgagc ttgtggccga acaggcccat ggagaagtcg gtgaacagct
     1801 tgatcatccc cgcctgctcg aggaacgagc cgaacaggat gaacaggaag atgtaggtgg
     1861 ccgacacgta ggtcggcgtg ccgtacaggc cctcggtgcc gaaggacagc tggttgacga
     1921 tctggtcgag gccgtagccg cggtgcgcca gctcgcccgg caggtattcg ccgaacaggc
     1981 cgtaggcgag gaacaggccg cagatcagcg gcagggcgac gcccatcacc cgccgcgccg
     2041 cctcgaacac cagggcgatc agcagcaggc cgatgaccat gtcggtatcg gtcagctcgc
     2101 cggagcgctg gatcagctcg gcctcgaaca cccactggta ggcgaaggtg ctcatgccgg
     2161 ccaggccgag cagccaggcc agcggctgga acggccgctt gctgccccag gccgggtagc
     2221 agaggaacac cagcagcagc aggaagccga cgtggccggc gcgcaggatc tggctggtca
     2281 ccggatggaa ggccgcggtg atgatctggt agatggagaa cagcagggcg acggcgaaca
     2341 gcgccttcgg ccactcactg gggctggcgt gcatgccttg gctttcgctc atgggatacc
     2401 tcggagcggg aaatcggaag ggacgggagc cgccaacaga acctgccgtg aaggttcacg
     2461 caggcttagt cggctcctct gcgggcgggg cgggcgcaga cgccgcgcct gaccgggccg
     2521 cgcgcggacg g
//
LOCUS       DATCUN010000278         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_87879, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000278 DATCUN010000000
VERSION     DATCUN010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87879"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1806
                     /gene="mshL"
                     /locus_tag="VIO83_10800"
     CDS             163..1806
                     /gene="mshL"
                     /locus_tag="VIO83_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HEY8331800.1"
                     /translation="MPMMPFRLSHLGLLATCCLLAACQTFTDGDRQLREQTDRLLEES
                     LAQSRRSNVPPPAVQAALLPPLLPDGAGFSSGPRFDVAVRDMEAREFFLSLMDGAGQN
                     LVVHPEVSGLITFSLRNVTLDEVLRAVRDTYGYDFKRTSYGYRILPNQLQTRTYNLNY
                     LNVQREGSSDTRVSSGQVTGSDDSNSDSSGNRSGSNNGNDSGDSGSGNGVSTVNASQV
                     LTSSKVDFWKEVGEVVGALIGDEEGNTVVVNPQASLLVVRASSSDQQNVEAFLEQAER
                     NLQRQVILEAKILEVRLSDSFQAGINWTQLGKEGNSTFGLALDGAELSGVQGIGGVFS
                     AALSVGDFSGLLQLLETQGQVRVLSSPRISTLNNQKAVIKVGSDEFFVTDVSSTNTTT
                     TTGIAEPTQDVTLTPFFSGISLDVTPQINQHEEVTLHVRPTVSRVQDQNKKISFGEDR
                     VLELPLALSTTRQSDSIVRARSGQVVVIGGLLEDVSDNTDANVPWLSKLPFLGVLFQQ
                     QRKDLVRSELVILLRPQVIGADTWLEELQKSAASFRELR"
     gene            1811..>2416
                     /locus_tag="VIO83_10805"
     CDS             1811..>2416
                     /locus_tag="VIO83_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8331801.1"
                     /translation="MYLSFFGLGEKPFALTPNTAFLVRLAPYQACLNLLRVALGEGEG
                     FIKVSGEVGTGKTLLCRALLNALSEDDGRWQVAWLPNPGLSPRDLRQALAHELRIADA
                     EHLDDHSLLAALNQRLIELAAEGKRTVLLIDEAQALPPKTLEALRLLTNLETEHCKLL
                     QVVLFGQPELDAVLEQPEFRQLRQRITFSYRLLEHGVQLRLA"
BASE COUNT          419 a          847 c          790 g          360 t
ORIGIN      
        1 ggtgctcggc gggcgcagcc tgcaggtcgg cgagcgccat ggcgacctgc gcgtgctggc
       61 catccgcgcg cgcagcgtac tgatcgaacg ccaggggcag cgcagtgagc tgcacctgat
      121 tcaacccatt ctgactccag ccacccggag cctgagccgc tgatgcccat gatgcctttt
      181 cgcctgtcgc accttggcct gctcgccacc tgctgcctgc tggccgcctg ccagaccttc
      241 accgatggcg accgccagct gcgcgagcag accgaccgcc tgctcgagga gagcctggcg
      301 cagagccggc gcagcaacgt gccgccgccg gccgtgcagg cggcgctgct gccaccgctg
      361 ctgccggacg gcgcgggctt ctccagcggc ccgcgtttcg acgtcgcggt gcgcgacatg
      421 gaggcccgcg agttctttct cagcctgatg gacggcgccg ggcagaacct ggtggtgcat
      481 cccgaggtca gcgggctgat caccttcagc ctgcgcaacg tcaccctcga cgaagtgctg
      541 cgcgcggtgc gcgataccta cggctacgac ttcaagcgca ccagctacgg ctaccgcatc
      601 ctgcccaacc agctgcagac gcgcacctac aacctcaact acctcaacgt gcagcgcgag
      661 ggttccagcg acacccgggt cagctccggc caggtcaccg gcagcgacga cagcaactcc
      721 gacagctccg gcaaccgttc gggcagcaac aacggcaacg acagtggcga cagtggcagc
      781 ggcaatggcg tcagcaccgt caacgccagc caggtgctga ccagcagcaa ggtcgacttc
      841 tggaaagagg tcggcgaggt ggtcggtgcg ctgatcggcg acgaggaagg caacacggtg
      901 gtggtcaacc cgcaggccag cctgctggtg gtgcgcgcct ccagcagcga ccagcagaac
      961 gtcgaggcct tcctcgagca ggccgagcgc aacctgcagc gccaggtgat cctcgaggcg
     1021 aagatcctcg aggtgcgcct gagcgacagc ttccaggccg gcatcaactg gacccagctg
     1081 ggcaaagagg gcaacagcac cttcggcctt gctctggatg gcgccgagtt gagcggcgtg
     1141 cagggcatcg gtggggtatt cagcgcggcg ctcagcgtcg gcgacttcag cggcctgctg
     1201 cagctgctgg aaacccaggg ccaggtgcgc gtgctgtcca gcccgcggat ctccacgctg
     1261 aacaaccaga aggcggtgat caaggtcggc tccgacgagt tcttcgtcac cgacgtgtcg
     1321 tcgaccaaca ccaccaccac caccgggatt gccgagccga cccaggatgt caccctgacg
     1381 ccgttcttct ccggcatctc gctggacgtg acgccgcaga tcaaccagca cgaggaggtc
     1441 accctgcacg tgcggccgac ggtcagtcgc gtgcaggacc agaacaagaa gatcagcttc
     1501 ggcgaggacc gcgtgctcga gctgccgctg gcgctgtcca ccacccgcca gtcagactcc
     1561 atcgtgcggg cgcgcagcgg ccaggtggtg gtgatcggcg gcctgctgga ggatgtcagc
     1621 gacaacaccg acgccaatgt gccctggctg tcgaagctgc cgttcctagg cgtgctgttc
     1681 cagcagcagc gcaaggacct ggtgcgcagc gagctggtga tcctcctgcg cccgcaggta
     1741 atcggcgccg atacctggct cgaagagctg cagaagagcg cggccagctt ccgcgagctg
     1801 aggtaggccc atgtacctga gcttcttcgg cctgggcgag aaacccttcg ccctcacccc
     1861 caacaccgcc tttctggtgc ggctggcgcc ctatcaggcc tgcctcaacc tgctgcgcgt
     1921 ggcgctgggc gagggcgaag gcttcatcaa ggtcagcggc gaggtgggca ccggcaagac
     1981 cctgctgtgc cgtgcgctgc tcaacgccct cagcgaggat gacgggcgct ggcaggtggc
     2041 ctggctgccc aaccccggcc tcagtccgcg cgacctgcgc caggcgctgg cccatgagct
     2101 gcgcatcgcc gatgccgagc atctggatga ccatagcctg ctggccgccc tcaaccagcg
     2161 gctgatcgag ctggcggccg agggcaagcg caccgtgctg ctgatcgacg aagcacaggc
     2221 cctgccgccg aagaccctgg aagcgctacg cctgctgacc aacctggaaa ccgagcactg
     2281 caagctgctg caggtggtgc tgttcggcca gccggagctg gacgccgtgc tcgagcagcc
     2341 ggagttccgc cagctgcgcc agcgcatcac cttctcctac cgcctgctcg agcacggcgt
     2401 ccagctccgg ctggcc
//
LOCUS       DATCUN010000279         5491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_126151, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000279 DATCUN010000000
VERSION     DATCUN010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126151"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VIO83_10810"
     CDS             <1..522
                     /locus_tag="VIO83_10810"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY8331802.1"
                     /translation="VLVADAADPQALNAAALALFEQALNELKSGRGREGAALAALLEE
                     RLVAMLAEVDNLRELVPQMLATQRQKILDRCAELQVELDPQRLEQELVMLAQKSDVAE
                     ELDRLSTHVREVRRVLKAGGAAGRRLDFLMQELNREANTLGSKAFDPRSTQAAVNLKV
                     LIEQMREQVQNIE"
     gene            545..1165
                     /gene="gmk"
                     /locus_tag="VIO83_10815"
     CDS             545..1165
                     /gene="gmk"
                     /locus_tag="VIO83_10815"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY8331803.1"
                     /translation="MSATSGTLYIVSAPSGAGKTSLVKALLDSNEQIRVSVSHTTRAM
                     RPGEVDGVNYHFVAREDFLGMLEQDAFLEHAEVFGNLYGTSQRWVEQTLAGGLDLILE
                     IDWQGAQQVRRLMPHAQSIFILPPSQEALRQRLNNRGQDSEAIIEQRMREAVSEMSHF
                     VEYDYVVINDQFSHALEDLKAIFRARQLHQCAQQQRHAGLLERLLR"
     gene            1268..1531
                     /gene="rpoZ"
                     /locus_tag="VIO83_10820"
     CDS             1268..1531
                     /gene="rpoZ"
                     /locus_tag="VIO83_10820"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY8331804.1"
                     /translation="MARVTVEDCLDNVDNRFELVMLSSKRARQLATGGKEPKVAWDND
                     KPTVVALREIAEGLVNYEVVAQEDIVEETPLFAAFEENNSEEI"
     gene            1591..3702
                     /gene="spoT"
                     /locus_tag="VIO83_10825"
     CDS             1591..3702
                     /gene="spoT"
                     /locus_tag="VIO83_10825"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY8331805.1"
                     /translation="MPSIDALADRLYSYLTTDQVNLVRRAYYYAEQAHDGQRRRSGEP
                     YVTHPLAVASILADMHMDHQSLMAAMLHDVIEDTGIPKDALSAQFGETVAELVDGVSK
                     LTQMDFQTKAEAQAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLAGEKRRRIAK
                     ETLEIYAPIANRLGMHNVFAEFEDLGFKAMHPMRAERIRQAVKKARGNRREIVGKIQE
                     SLTNCLTQEGLEGVVTGREKHLYSIYQKMRGKRRAFNEIMDVYAFRITVDKVDTCYRV
                     LGAVHSLYKPFPGRFKDYIAIPKANGYQSLHTTLFGMHGVPIEIQIRTREMEEMAKHG
                     IAAHWLYKSNEEESPRGSHARARQWVKGILELQQRAGNSLEFIENVKIDLFPDEVYVF
                     TPKGRIMELPKGSTAVDFAYAVHTDVGNSCIACRINRRLAPLSEQLQSGATVEIVTAP
                     GARPNPAWLNFVVTGKARTHIRHSLKQQRRSESVSLGERLLNKALAGFDSHLERIAAE
                     RLAAVLAEYQQDSLDDLLEDIGLGNRMAYVVARRLLAEGGSEQMPSHEGPLAIRGTEG
                     LVISYAKCCTPIPGDPIVGHLSAGKGMVIHLDGCHNISEFRHNPDKCIPLSWAKDVSG
                     EFNVELRVELEHQRGLIALLATAVASADGNIEKISMDERDGRISVVQLVVSVHDRVHL
                     ARVIKRLRTIKEVTRITRLRA"
     gene            3782..4165
                     /locus_tag="VIO83_10830"
     CDS             3782..4165
                     /locus_tag="VIO83_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8331806.1"
                     /translation="MSKSVISTDQAPAAIGTYSQAIKAGNTVYLSGQIPLVPATMQMV
                     EGGFEAEAVQVFENLKAVAAAAGGSLADTAKLNIFLTDLGNFATVNEVMSRYFQPPYP
                     ARAAIGVAALPRGAQVEMDAILVLA"
     gene            4237..4965
                     /locus_tag="VIO83_10835"
     CDS             4237..4965
                     /locus_tag="VIO83_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331807.1"
                     /translation="MRHALPFLLCTALLGGCASGPDDIAGTWINQPAIEAAADSGKLR
                     EAMLAYGPTLEWRFAPQRHTAWSSNGAEFGEGHLAPEGDAHWRVTFHGDYQQGFAVDG
                     DELVQQPSASAPEQRFSLAETAADGLPGQAFEQALYAALLGGDWEIREGAGQQGRVRF
                     HPDGRVEGLPGVERYALCLAGDCAERSEEQEIIWLQNAEQGREWLFQLADDELSIYTA
                     DNQALHSDEPQYRPGRRAWLLERD"
     gene            complement(5067..>5491)
                     /locus_tag="VIO83_10840"
     CDS             complement(5067..>5491)
                     /locus_tag="VIO83_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8331808.1"
                     /translation="GIPASVVRLAGIYGPGRRWLLSQVRAGYRVSSEPPLYGNRIHAD
                     DAAGLLACLLQADARGVELEAVYLGVDDEPAPLHEVIAWLRGQLGVSHWAAESTVRRA
                     GSKRCSNARARALGWVPHYPSYREGYAALLESVAECPR"
BASE COUNT         1018 a         1990 c         1724 g          759 t
ORIGIN      
        1 gtgctggtgg ccgacgccgc cgatccgcag gcgctgaacg ccgccgccct ggcgctgttc
       61 gagcaggcgc tgaacgagct gaagagcggc cgcggccgcg aaggcgccgc cctggccgcg
      121 ctgctcgagg agcgcctggt cgcgatgctt gccgaggtcg acaacctgcg cgagctggtg
      181 ccgcagatgc tcgccaccca gcggcagaag atcctcgacc gctgcgccga actgcaggtc
      241 gaactggacc cgcagcgcct ggagcaggaa ctggtgatgc tggcgcagaa gagtgacgtg
      301 gccgaggagc tggatcgtct gagcacccat gtgcgcgagg tgcgccgcgt gctcaaggcc
      361 ggcggcgccg ccggtcggcg gctggacttc ctgatgcagg aactcaaccg cgaggccaac
      421 accctcggct ccaaggcctt cgacccgcgc agcacgcagg ctgcggtcaa cctcaaggtg
      481 ttgatcgagc agatgcgcga acaagtgcag aacatcgagt aatcccgcag tgacaggaac
      541 ccaaatgagc gccacgtccg gcaccctcta catcgtttcg gcgccctccg gtgccggcaa
      601 gaccagcctg gtcaaggcgc tgctcgacag caacgaacag atccgcgtct cggtgtcgca
      661 caccacccgc gccatgcgcc cgggcgaggt ggacggggtg aactaccact tcgtcgcccg
      721 cgaggacttt ctcggcatgc tcgagcagga cgccttcctc gagcacgccg aggtgttcgg
      781 caatctctac ggcacctcgc agcgctgggt cgagcagacc ctcgccggcg gcctcgacct
      841 gatcctcgag atcgactggc agggcgccca gcaggtgcgc cggctgatgc cgcacgcgca
      901 gtcgatcttc atcctgccgc cgtcgcagga ggcgctgcgc cagcgtctga acaaccgcgg
      961 ccaggacagc gaggcgatca tcgagcagcg catgcgcgag gcggtcagcg aaatgagcca
     1021 cttcgtcgag tatgactatg tggtaatcaa cgaccagttc agccatgctc tggaagacct
     1081 caaggcgatc ttccgcgccc gccagctgca ccagtgcgcc cagcagcagc gccatgccgg
     1141 gttgctcgaa cgcctgttgc gctgaagcgc caacgcattc ggcacttccc taaccattgg
     1201 cgatttttta gactatccaa tccgcccggc actcccgggc ccagccgttg cgataaagag
     1261 gaacaccatg gcccgcgtca ccgtcgaaga ctgcctggac aacgtcgata accgttttga
     1321 actggtcatg ctctccagca agcgcgcccg ccagctggcg accggcggca aggagccgaa
     1381 agtagcctgg gacaacgaca agccgaccgt ggtggccctg cgtgaaatcg ccgaaggcct
     1441 ggtcaactat gaagtagtcg cgcaggaaga catcgtcgag gaaactccgc tgtttgctgc
     1501 cttcgaggaa aacaacagcg aggagatctg aattgccagg tcgacgtcgc gcgcctgaag
     1561 taccggcaca gtccggtctg gagtgacccc ttgcccagca tagacgccct cgccgacagg
     1621 ctttacagct acctcacgac ggaccaggtc aacctggtcc gccgcgccta ctactacgcc
     1681 gagcaggccc atgacggcca gcgccggcgc agcggcgaac cctacgtcac ccatccgctg
     1741 gccgtggcca gcatcctcgc cgacatgcac atggaccatc agagcctgat ggccgccatg
     1801 ctgcacgacg tcatcgagga caccggcatc cccaaggacg ccctcagcgc gcaattcggc
     1861 gagaccgtcg ccgaactggt cgacggggtc agcaagctga cccagatgga cttccagacc
     1921 aaggccgagg cgcaggcgga aaacttccag aagatggcca tggccatggc ccgcgacatc
     1981 cgcgtgatcc tggtcaagct ggccgaccgc ctgcacaaca tgcgcaccct cgaggtgctg
     2041 gccggcgaga agcgccggcg catcgccaag gaaaccctgg agatctacgc gccgatcgcc
     2101 aaccgcctgg gcatgcacaa cgtgttcgcg gagttcgagg acctcggctt caaggctatg
     2161 cacccgatgc gcgccgaacg catccgccag gcagtgaaaa aggcgcgcgg caaccgccgc
     2221 gagatcgtcg gcaagatcca ggaatcgctg accaactgcc tgacccagga aggcctggaa
     2281 ggggtggtca ccgggcgcga gaagcacctc tacagcatct accagaagat gcgtggcaag
     2341 cgccgggcgt tcaacgagat catggacgtc tacgccttcc gcatcaccgt cgacaaggtc
     2401 gacacctgct accgggtgct cggcgcggtg cacagcctgt acaagccgtt ccccggccgc
     2461 ttcaaggact acatcgcgat ccccaaggcc aacggctacc agtcgctgca caccaccctg
     2521 ttcggcatgc acggcgtgcc catcgagatc cagatccgca cccgcgagat ggaagagatg
     2581 gccaagcacg gcatcgccgc gcactggctg tacaagtcca acgaggagga aagcccgcgc
     2641 ggcagccacg cccgtgcccg ccagtgggtc aagggcatcc tcgaactaca gcaacgcgca
     2701 ggcaactccc tcgagttcat cgagaacgtg aagatcgacc tgtttcccga cgaggtctac
     2761 gtgttcaccc ccaagggccg catcatggaa ctgcccaagg gttccacggc ggtcgacttc
     2821 gcctatgcgg tgcacaccga cgtcggcaac agctgcatcg cctgccgcat caaccgccgc
     2881 ctggcgccgt tgtccgagca gctgcagagc ggtgcgacgg tggagatcgt caccgccccg
     2941 ggcgcgcggc cgaatccggc ctggctcaac ttcgtggtca ccggcaaggc gcgcacccac
     3001 atccgccatt cgctcaagca gcagcgccgc tcggagtcgg tcagcctcgg cgagcgtctg
     3061 ctcaacaagg cgctggccgg cttcgacagc cacctcgagc ggatcgccgc cgagcgcctc
     3121 gccgcggtgc tcgccgaata ccagcaggac agcctcgacg acctgctcga ggacatcggc
     3181 ctcggcaacc gcatggccta cgtggtggcg cgccgcctgc tggccgaagg cggcagcgag
     3241 cagatgccca gccacgaagg cccgctggcg atccgcggca ccgagggcct ggtgatcagc
     3301 tacgccaagt gctgcacacc gatccccggc gaccccatcg tcggccacct gtcggccggc
     3361 aagggcatgg tcatccacct cgatggctgc cacaacatca gcgaattccg ccacaacccg
     3421 gacaagtgca tcccgctgtc ctgggccaag gacgtcagcg gcgagttcaa cgtcgagctg
     3481 cgcgtcgagc tggagcacca gcgcggcctg atcgccctgc tggccaccgc ggtggccagc
     3541 gccgacggca acatcgagaa gatcagcatg gacgagcgcg acggccgcat cagcgtggtc
     3601 cagctggtgg tcagcgtgca cgaccgcgtg cacctggcgc gggtcatcaa gcgcctgcgc
     3661 acgatcaaag aggtcacccg catcacccgc ctgcgcgcct gagccgcgcc ggccagcctc
     3721 accggcagcg cgcccgccgc accccggcgc ctgccttcgc caccctctgc aaggagctcc
     3781 catgagcaag agcgtcatca gcaccgacca ggccccggcg gccatcggca cctactccca
     3841 ggccatcaag gccggcaaca ccgtctacct gtccgggcag atcccgctgg tgccggccac
     3901 catgcagatg gtcgaaggcg gcttcgaggc cgaggccgtg caggtgttcg agaacctcaa
     3961 ggccgtcgcc gcggccgccg gtggctcgct ggccgatacc gccaagttga acatcttcct
     4021 caccgatctc ggcaacttcg ccaccgtcaa cgaggtgatg agccgctact tccagccgcc
     4081 ctacccggcg cgcgccgcca tcggcgtggc cgccctgccg cgcggtgccc aggtggagat
     4141 ggacgccatt ctcgtcctcg cctgatcccc acggtgcgcc ggacccgcgc cggcgcaccg
     4201 cgacatcccc ccgtttttcc cggaaaggac gcccccatgc gccatgccct gccctttctg
     4261 ctgtgcaccg ccctgctcgg cggctgcgcc agcggaccgg acgatatcgc cggcacctgg
     4321 atcaaccagc cggccatcga ggccgccgcc gacagcggca agctgcgcga ggccatgctc
     4381 gcctacggcc cgaccctgga atggcgcttc gcgccacagc ggcacaccgc ctggtcgagc
     4441 aacggcgccg aattcggcga gggccacctg gcaccggaag gcgacgcgca ctggcgggtg
     4501 accttccacg gcgactatca gcaaggtttc gccgtcgacg gcgacgagct ggttcagcag
     4561 cccagcgcca gcgcgcccga gcaacgcttc agcctcgccg aaacggccgc cgacggcctg
     4621 cccggccagg ccttcgaaca ggcgctgtac gccgccctgc tcggcggcga ctgggaaatc
     4681 cgcgaaggcg ccggccagca gggccgggtg cgcttccacc ccgacggccg ggtcgagggc
     4741 ctgcccggtg tcgaacgcta cgccctgtgc ctggccggcg actgcgccga acgcagcgag
     4801 gagcaggaga tcatctggct acagaacgcc gagcaggggc gcgaatggct gttccagctg
     4861 gccgacgacg agctgagcat ctacacggcg gacaaccagg ccctgcacag cgacgagccg
     4921 cagtaccgcc ccggccgaag ggcctggctg ctggaacgcg attagtggcc tgtttggtta
     4981 gttctgtcag tcagtttcgg cggccatggc cccgataccg cgttaaccaa ctgaccgcac
     5041 aggccactcg cccttagccc tgccgctcac cgagggcatt cggccaccga ctccagcagc
     5101 gccgcgtagc cctcgcgata gctcggatag tgcggcaccc agcccagcgc acgggcccgg
     5161 gcgttgctgc agcgcttgct gccggcgcgg cggaccgtcg actcggccgc ccagtggctg
     5221 acgcccagtt gcccgcgcaa ccaggcaatc acttcatgca acggcgccgg ctcgtcgtcg
     5281 acgccgagat acaccgcctc cagctccacc ccgcgggcat cggcctgcaa caggcaggcc
     5341 agcaggccgg ccgcatcgtc ggcgtggatg cgattgccat acagcggcgg ctcgctgctc
     5401 acccggtagc cggcacgcac ctggctgagc agccagcgcc ggcccggccc gtagatgccg
     5461 gccagccgca ccacgctggc gggaatgccg c
//
LOCUS       DATCUN010000280         4551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_201491, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000280 DATCUN010000000
VERSION     DATCUN010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201491"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VIO83_10845"
     CDS             complement(<1..968)
                     /locus_tag="VIO83_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HEY8331809.1"
                     /translation="MKQKNLKTWFKSGSPWVWMTGGAVSIAVIMTLGLLAVIAVRGLG
                     HFWPADVLEATYRIPGQEPKVIAGEMVQAEEVPRARLAATGLPVDTAGGEFMTRELLK
                     VGNREVYGADFNWVVGEWLHEPHTPEQLVVLERREWGNFYGYLQGVKEAGKLVAEGDA
                     AWAALQARIDRVDELHAQIARLEKKDIGRINAGLERLRLQTRELELNGKLDAVAQADL
                     DAERAQWDAEYKVLEEQLVALQQQFNRDSIMVKTVDGRELELSLGKVVRAYQPNGMGI
                     FDKLGFYFAKLWEFVSDEPREANTEGGIFPAIFGTVMMTLIMAVIVT"
     gene            complement(996..3281)
                     /locus_tag="VIO83_10850"
     CDS             complement(996..3281)
                     /locus_tag="VIO83_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8331810.1"
                     /translation="MNDLANETMTSSSKSQRLDFNTPALQRKRRMRALKDHLARWYVS
                     IGGLAVLGAITLIFFYLTSVVLPIFGGAELEARDAQQPAWLAQQDKPLLLAIEEQNQV
                     AMRLDRAGQVQFFELKNGKALNRASLPLPAGVAVTSVVEDQPGSRRVALGLSNGQALV
                     LQHNYKVTYPDNVKTIVGAIDYPFGEAPIALDPQGRALERVGLSLNDSTLMLAASTGS
                     ELHVLSLSREENLFTGEVTVAEQRLSLPQIADPIRALVVDPRQQWLYVFNGRATADVF
                     DLRTNSLNGRYNLARDGETEISSATSLLGGISLMVGDSKGGIAQWFMVRDQEGKAALT
                     RIREFQLGDSPVTQILAEERRKGFLALDAAGTLGIFHSTAHRTLLTQQVAEGAAVAAL
                     SPRATRVLVESNGQLQRFVIDNPHPEVSWSALWGKVWYESYDEPKYVWQSTSANTDFE
                     PKLSLAPLTFGTLKAAFYAMLLAAPLAICAAIYTAYFMAPAMRRKVKPVIELMEALPT
                     VILGFFAGLFLAPYVEGHLPGIFSLLLLTPVGILLAAWLWSRLPESIRLRVPDGWEAA
                     LLIPVILGVAALSLGMSGHMENWFFGGDMRLWLSNDLGIAFDQRNALVVGLAMGFAVI
                     PNIFSIAEDAVFSVPKSLTFGSLALGATPWQTLTRVVILTASPGIFSALMIGLGRAVG
                     ETMIVLMATGNTPIMDMNIFEGLRTLAANTAVEMPESEVGSSHYRVLFLAALVLLAFT
                     FVMNTLAELIRTRLRKKYASL"
     gene            complement(3420..4388)
                     /locus_tag="VIO83_10855"
     CDS             complement(3420..4388)
                     /locus_tag="VIO83_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEY8331811.1"
                     /translation="MKFKRLMAAMTFVAAGVATASAVAAIDPALPAYQKASGVSGNLS
                     SVGSDSLANLMTLWAEDYKKFYPNVNIQIQAAGSSTAPPALTEGTANLGPMSREMKDN
                     ELQAFEEKHGYKPTAVPVAIDALAVFVHKDNPIKSLDIAQVDAIFSSTRLCGGAKDLK
                     TWGDLGLTGEWAAKPIQLFGRNSVSGTYGYFKESALCKGDFKANVNEQPGSASVVQSI
                     SSTLNGIGYSGIGYKTSSVRAVPLSKKGGEAFEANEANALAGKFPLARYFYVYVNKAP
                     NKPLSPLDAEFIKLVLSKQGQEVVVKDGYIPLPSKIAAKALKDLGL"
BASE COUNT          756 a         1455 c         1533 g          807 t
ORIGIN      
        1 gtgacgatca ccgccatgat cagggtcatc atcaccgtac cgaagatcgc cgggaagatg
       61 ccgccttcgg tgttggcctc gcgcggctcg tcgctgacga actcccacag cttggcgaag
      121 tagaagccca gcttgtcgaa gatgcccatg ccattcggct ggtaggcacg caccaccttg
      181 cccaggctca gctccagctc gcgaccgtcg acggtcttga ccatgatgct gtcgcggttg
      241 aactgctgct gcagggcgac cagctgctcc tcgagcacct tgtactcggc gtcccactgg
      301 gcgcgctcgg cgtccaggtc ggcctgggcg acggcatcca gcttgccgtt caactccagc
      361 tcgcgggtct gcagacgcag gcgctccaga ccggcgttga tgcggccgat gtccttcttc
      421 tccaggcggg cgatctgcgc gtgcagctcg tcgacacggt cgatgcgcgc ctgcagcgcg
      481 gcccaggccg catcgccttc ggcgaccagc ttgccggctt ccttgacgcc ctgcaggtag
      541 ccgtagaagt tgccccactc gcggcgctcg agcaccacca gctgctcggg ggtgtgcggc
      601 tcgtgcagcc actcgccgac cacccagttg aagtcggcgc catagacctc gcggttgccg
      661 accttgagca gctcgcgggt catgaactcg ccgcccgcgg tgtccaccgg caggcccgtg
      721 gcggcgaggc gcgcacgcgg cacctcttcg gcctggacca tctcgccggc gatcaccttc
      781 ggctcctggc cggggatgcg gtaggtggct tccagcacgt cagccggcca gaagtggccg
      841 aggccgcgca cggcgatgac cgccagcagg ccgagggtca tgatcaccgc gatggacacc
      901 gcgccaccgg tcatccatac ccacggcgaa ccgctcttga accaggtttt caggtttttc
      961 tgtttcacgt tcacggacgt ctaccttccc gagtcttaga gcgaggcata tttcttgcgc
     1021 aggcgggtac gaatgagctc cgccagggtg ttcatcacga aggtgaaggc cagcagcacc
     1081 agcgcagcga ggaacagcac gcggtagtgg ctgctgccga cttccgactc gggcatttcc
     1141 accgccgtgt tggcggccag ggtgcgcaga ccttcgaaga tgttcatgtc catgatcggc
     1201 gtgttgccgg tggccatcag cacgatcatg gtctcgccca ccgcccggcc gaggccgatc
     1261 atcagtgccg agaagatgcc cgggctggcg gtgaggatca ccacgcgggt cagggtctgc
     1321 cacggcgtgg cgccgagggc cagggagccg aaggtcaggc tcttcggcac gctgaacacg
     1381 gcgtcttcgg cgatcgagaa gatgttcggg atcaccgcga agcccatcgc caggccgact
     1441 accagggcgt tgcgctggtc gaaggcgatg cccaggtcgt tggacagcca caggcgcatg
     1501 tcgccgccga agaaccagtt ctccatgtgg ccgctcatgc ccagcgacag ggcggcgacg
     1561 ccgaggatca ccgggatcag cagcgccgct tcccagccgt ccggcacgcg caggcggatg
     1621 ctctccggca ggcggctcca cagccaggcg gcgaggagga tgccgaccgg ggtcagcagc
     1681 agcaggctga agatgcccgg caggtggcct tccacatagg gcgcgaggaa caggccggcg
     1741 aagaagccga ggatcaccgt cggcagcgct tccatcagct cgatcaccgg cttgaccttg
     1801 cgacgcatcg ccggggccat gaagtaggcg gtgtagatcg ccgcgcagat cgccagcggg
     1861 gcggccagca gcatggcgta gaaggccgcc ttgagggtgc cgaaggtcag cggcgccagg
     1921 ctcagcttgg gctcgaagtc ggtgttggcc gaggtcgact gccagacgta cttgggctcg
     1981 tcatagctct cgtaccagac cttgccccac agcgcgctcc acgacacttc cgggtgcggg
     2041 ttgtcgatca cgaagcgctg cagctggccg ttgctctcca ccagcacgcg ggtggcacgc
     2101 ggcgacaggg cggcgaccgc ggcgccttcg gcgacctgct gggtcagcag ggtgcggtgc
     2161 gcggtgctgt ggaagatgcc cagggtgcct gcggcgtcga gggcgaggaa gcccttgcgg
     2221 cgctcttcgg cgaggatctg ggtcaccggg ctgtcgccca gctggaactc gcggatgcgg
     2281 gtcagggccg ccttgccttc ctggtcgcgg accatgaacc actgggcgat gccgcctttc
     2341 gagtcgccga ccatcagcga gatgccgccg agcagcgaag tggcgctgga gatctcggtt
     2401 tcgccgtcgc gggccaggtt atagcgaccg ttcaggctgt tggtgcgcag gtcgaacacg
     2461 tcggcggtgg cgcggccgtt gaacacgtac agccactgct ggcgcggatc gacgaccagc
     2521 gcgcgaatcg ggtcggcgat ctgcggcagg ctcaggcgct gctcggcgac agtgacttcg
     2581 ccggtgaaca gattctcttc gcgactcagg ctcagcacat gcagctcgct gccggtggag
     2641 gcggccagca tcagggtcga atcgttgagg ctgaggccca cacgctccag cgcgcgaccc
     2701 tgcgggtcga gggcgatggg cgcctcaccg aacggatagt cgatcgcccc gacgatggtc
     2761 ttgacgttgt ccggataggt gaccttgtag ttgtgctgca gcaccagtgc ctggccgttg
     2821 gacaggccca gcgccacgcg gcggctaccc ggctggtcct cgaccaccga ggtgacggcc
     2881 acgccggccg gcagcggcag gctggcgcga ttcagcgcct tgccgttctt cagttcgaag
     2941 aattgcacct ggccggcccg atccaggcgc atggccacct gattctgctc ctcaatggcg
     3001 agcagcagcg gcttgtcctg ctgcgccagc caggccggct gctgcgcatc acgcgcctcc
     3061 agctcggcac cgccgaagat cggcagcacg acgctggtca gatagaagaa gatcagggta
     3121 atcgcgccaa ggacggcgag accgccaatg gacacgtacc aacgcgccag gtggtccttg
     3181 agcgcgcgca tgcgacgctt gcgctggagc gccggcgtat tgaaatccag tcgttgcgat
     3241 tttgaggagg aagtcatggt ttcattggcc aagtcattca tgcgcacacc ctaacggggg
     3301 tttatgacac aaagattaca gggtggtgac gcagcaacgc ccgccactct cgggaggggc
     3361 gggcgttgct ctagctcggg cttacgcccg tggcatccgt gccactgtca gtcgtcgact
     3421 tacaggccaa gatccttcag agctttcgcg gcgatcttgc tcggcagcgg gatgtagccg
     3481 tccttgacga ccacttcctg accctgcttg gacagtacca gcttgatgaa ctcggcatcc
     3541 agcgggctca gcggcttgtt cggcgccttg ttcacgtaga cgtagaagta gcgcgccagc
     3601 gggaacttgc cggccagggc gttggcttcg ttggcttcga aggcttcgcc gcccttcttc
     3661 gacagcggta cggcgcggac gctggaggtc ttgtagccga tgcccgagta gccgatgccg
     3721 ttcagggtgc tggagatcga ctgcaccacc gaggcggagc ccggctgctc gttgacgttg
     3781 gccttgaagt cacccttgca cagcgcggat tccttgaagt agccgtaggt accggacacc
     3841 gagttgcgac cgaacagctg gatcggcttg gccgcccact cgccggtcag gcccaggtcg
     3901 ccccaggtct tgaggtcctt ggcgccaccg cacaggcggg tgctggagaa gatggcgtca
     3961 acctgggcga tgtccaggct cttgatcggg ttgtccttgt gcacgaacac ggccagggca
     4021 tcgatggcta ccggcacggc ggtcggcttg tagccgtgct tctcttcgaa ggcctgcagc
     4081 tcgttgtcct tcatctcgcg gctcatcggg ccgaggttgg cagtgccctc ggtcagcgcg
     4141 ggcggcgcgg tggaggaacc ggcagcctgg atctggatgt tgacgttcgg gtagaacttc
     4201 ttgtagtcct cggcccacag ggtcatcagg ttggccaggg agtcggagcc gacgctggac
     4261 aggttgccgg acacgccgct ggccttctgg taggccggca gagccgggtc aatggcagcg
     4321 accgcgctgg cggtagcgac gccggcggca acgaaggtca tggccgccat caaacgcttg
     4381 aatttcatgc cttgctccta acggattgga atggtcttga tcgggggcca gtatcggcag
     4441 gccgtatgaa cactctatga atcgattgtg acaattgcgt gaccgggcat ttcgccaccg
     4501 gcaaccgtcc ggcggtcgtt tacatccgcc actgccggta tactctgcac a
//
LOCUS       DATCUN010000281        11681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_69259, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000281 DATCUN010000000
VERSION     DATCUN010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11681
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69259"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     repeat_region   15..637
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=15..45
                     /rpt_unit_seq="gtttcaatccacgcgctcgtgagagcgcgac"
     gene            complement(812..1102)
                     /gene="cas2"
                     /locus_tag="VIO83_10860"
     CDS             complement(812..1102)
                     /gene="cas2"
                     /locus_tag="VIO83_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEY8331812.1"
                     /translation="MMVLVSYDVSTIAPGGEKRLRRVAKACRDVGQRVQYSVFEIEVE
                     PAQWVQLRQRLCDLIDPECDSLRFYQLGAKWEGRVEHIGAKPSVNFKGPLIF"
     gene            complement(1119..2153)
                     /gene="cas1c"
                     /locus_tag="VIO83_10865"
     CDS             complement(1119..2153)
                     /gene="cas1c"
                     /locus_tag="VIO83_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HEY8331813.1"
                     /translation="MRRQLNTLYATTDGAWLRKDGANIVLEVERQERARLPVHMLESL
                     VCIGRVAVSPQLLGFCSAQGISICYLTPQGRFLARVEGPVSGNVLLRREQYRRSDDPS
                     RCAAIVRNLLAGKIHNQRAVLARGWRDHGDRLADAPAFQHALKRLRRIPQRLLLENDV
                     DILRGLEGEAAQAYFGVFDQLVRAESPLLRFGGRNRRPPRDALNALLSFLYTLLTHDC
                     RSALETVGLDPAVGFLHRDRPGRPSLALDLAEEFRPLLGERLALSLINRRQLNERDFQ
                     VFDNGAVQLKDEARKNVLVAYQERKREQLQHAFLGEKVDIGLLPFVQAQLLARHLRGD
                     LDGYPPFLWQ"
     gene            complement(2156..2788)
                     /gene="cas4"
                     /locus_tag="VIO83_10870"
     CDS             complement(2156..2788)
                     /gene="cas4"
                     /locus_tag="VIO83_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HEY8331814.1"
                     /translation="MDDEDLIPLSALQHYLYCPRQCALIHLEQQWKENRQTAEGRLLH
                     ERADVPQAERRRGVRTVTAMPLLNLQLGITGKADVVEFHHTESGERVFPVEYKRGRPK
                     SHRADEVQLCAQGLCLEAMLDTHVYAGALFYGQTRRRKDVTFDQELRALTRATITGTR
                     AMLALGKTPSARYEAKRCDPCSLLDLCQPRLLGRRSVQAWLQRQLEHEEE"
     gene            complement(2811..3701)
                     /gene="cas7c"
                     /locus_tag="VIO83_10875"
                     /pseudo
     CDS             complement(2811..3701)
                     /gene="cas7c"
                     /locus_tag="VIO83_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777774.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
     gene            complement(3698..5533)
                     /gene="cas8c"
                     /locus_tag="VIO83_10880"
     CDS             complement(3698..5533)
                     /gene="cas8c"
                     /locus_tag="VIO83_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HEY8331815.1"
                     /translation="MILSALDEYYRRLSDDRESGIAAPGYSQEKIGYAIVLDADGRVV
                     DVQDLRDSSGKKPAPRLLGVPQPEKRTVAVKSNFLWDKTSYVLGIGKNTARTQQDHAA
                     FKTLHRQALAGSEDPGLKALLAFLDDWTPAQFADNPLFSRHGEALLDANLVFRFDDGT
                     QQFLHQRQAARDAWNRQQALGADGVRGMCLVTGQQAPMARLHPAIKGVNGAQSSGASI
                     VSFNLDAFTSYGKSQGENAPVSEQAAFAYTTALNHLLRRTEDNHQRLQLGDTTVVFWA
                     QGAGAQAAEDFVATFFGTQQPDSEGLDDAQATRRLAQALEQLRQLRPLRELDSRLDED
                     TRIFVLGLAPNASRLSIRFWETTSLSSFANRLADHYRDLALEPSAWRRPPTPQYLALQ
                     TAPVYGENGRPKAEDVPPLLAGELTRAILTGMRYPRSILGAIVMRFRADGQISPLRVA
                     LCKAVLAREARLGMHHGTSTNKGEPPVSLDTSNTDPGYLLGRLFSSLENLQRAALGAQ
                     VNATIRDRYYGAASATPASIFPVLLRNAQNHFGKLRKDKAGLAMSLEKEIGQIVDSFP
                     PSLPRSLLIEAQGRFAIGYYHQTQARFARSGNQDAPLTDSEGETA"
     gene            complement(5530..6204)
                     /gene="cas5c"
                     /locus_tag="VIO83_10885"
     CDS             complement(5530..6204)
                     /gene="cas5c"
                     /locus_tag="VIO83_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HEY8331816.1"
                     /translation="MSYGVKLHVWGERALFTRPEMKVERVSYDVITPSAARGILEAIH
                     WKPAIRWVVDGIQVLRPVRFESIRRNEVGGKLSAASVGRAIRAGRTDGLVNYVEEDRQ
                     QRAATLLRDVGYVIAAHFELTARAGPEDSVGKHLDIFNRRARSGQCFQAPCLGTREFP
                     ASFALVENGYDLPIADASLQGERDLGWMLHDIDFADGMTPRFFRARMVDGLIQVPPPG
                     DEGVHG"
     gene            complement(6402..8636)
                     /locus_tag="VIO83_10890"
     CDS             complement(6402..8636)
                     /locus_tag="VIO83_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HEY8331817.1"
                     /translation="MEFYAHSTDRKDCSDWQGLRAHLLGVAERASANAAPFGGQELGW
                     IAGALHDLGKYTRRFQDRLRGEVPRVDHATWGARIAQQRYKGLGTLMAYAIAGHHAGL
                     ANGTLGEARSCLRERLSEDYLLRELPPLLPEWEHELELPQRLALPTRFSSVSTRPAFQ
                     FSLLARMVFSCLVDADFVDTDNFYRRIEGRDPRPERTGPSLDVLRAKLDAHLVSLPRQ
                     GGINPLRADILKHVRAQATHKPGLFSLTVPTGGGKTLASLAFALDHAIAHGLRRVIYV
                     VPFTSIIEQTAAVFRSAFSELGEDAVLEHHSAFFDDPKSELQSKEKRHLAMENWDAPI
                     IVTTAVQFFESLFADRPARCRKLHNIAASVVILDEAQTLPLHLLRPCMQALDELALNY
                     RVSMMLCTATQPALGKADGFKDGLEGVRELAPDPPGLYRRLKRVSVQHAGPLDDAALA
                     GHLRSREQILCIVNNRRHARALFDAIADLPGACHLTTLMYARHRSQVLQDLRTRLKAG
                     QPCRLVSTSLIEAGVDVSFPAVLRAEAGLDSIAQAAGRCNRNMEWAVEDSQVLVFESP
                     DWAPPKELKLFAEAFRMIERRHRADLLSLEAVTDYFRELYWRRDGQLGEMLLQLFDGA
                     TVDDLPLETLAARFRMIEANMRPVIVPYDPQTGAEDPFVASALRDLEDAPGTARKLQP
                     FLVQVPRQGYDALWNAGAIQPVAPERYGEQFVRLVNPRLYHPKFGLHWDDNPQFLAAE
                     ALVG"
     gene            8779..9489
                     /gene="ung"
                     /locus_tag="VIO83_10895"
     CDS             8779..9489
                     /gene="ung"
                     /locus_tag="VIO83_10895"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY8331818.1"
                     /translation="MSDPEPRIQLEPSWKARVGDWLLRPEMQQLSAFLRQRKAAGVHV
                     FPPGPQIFAAFEATPFDKVKVVILGQDPYHGAGQAHGLCFSVPPGVPVPPSLQNIYKE
                     LASDVGVAPPDHGCLSPWARRGVLLLNAVLTVEEGRPGAHRGKGWEGFTDQVIQALAA
                     EREHLVFLLWGSYAQAKGKVIDPARHRLLKAPHPSPLSAHRGFLGCRHFSQANQYLER
                     HGLGAVDWTLPRRAQLEA"
     gene            complement(9497..10144)
                     /locus_tag="VIO83_10900"
     CDS             complement(9497..10144)
                     /locus_tag="VIO83_10900"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEY8331819.1"
                     /translation="MPYPRFAPVLLLMSLMAAIAPPALAREPTDEEIERLLTASRAQS
                     MLAGIQPQVEAMQREQFAQLTQGKTLSPEQQAEIQQIQLKTSNIVRQALAWESMRPVY
                     LDVYRKTFDDDDVKAMTRFYESRAGQHLLDRTPVMMQTLMVAIQQKMIPQLEALQAEV
                     KTVSAEPLPAPPVSPPEARKKPATSSTRKKSTSSKKSSSSKSGTATKTTTPQKEP"
     gene            10273..10704
                     /gene="arfB"
                     /locus_tag="VIO83_10905"
     CDS             10273..10704
                     /gene="arfB"
                     /locus_tag="VIO83_10905"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY8331820.1"
                     /translation="MAAGSLQITPQLTIPDDELVERFVRASGAGGQNVDKVATAVELR
                     FDVAGSPSLPELLRSRLLARRDRRLTDEGVLVIEAQRFRTQDRNRQDARERLAQIIAQ
                     GLSVPKPRVATRPTRASRLRRLDAKKTRSQVKRNRNTRDWD"
     gene            10691..11311
                     /locus_tag="VIO83_10910"
     CDS             10691..11311
                     /locus_tag="VIO83_10910"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8331821.1"
                     /translation="MTGTEPTPRPPGQIPVAPPNMPRVKPNRFTRWCGRSLLRLCGWR
                     IAGELPDVPKLVMIVAPHSSNWDGLWGMAVKIACGFEVKVLGKAQLFWWPLGPLLRRL
                     GAIPVDRANAGGVVRQAVDAIRAAPRIWFVVTPEGTRKRVEKWKSGFYKIAVSAQVPI
                     LLAYFHYPEKIVGLGPLFHPTGDAEADMAAIRAWYAPWMGKHRGST"
     gene            complement(11542..>11681)
                     /locus_tag="VIO83_10915"
     CDS             complement(11542..>11681)
                     /locus_tag="VIO83_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEY8331822.1"
                     /translation="AIDEYLAHHNTKPKPFIWTRSARDILQKVIRANQRLSSKQNGTL
                     H"
BASE COUNT         1899 a         3755 c         3911 g         2116 t
ORIGIN      
        1 atcgccggcc tgcagtttca atccacgcgc tcgtgagagc gcgacccgcc ggcccggagt
       61 gggctggcgg ggagcattgg tttcaatcca cgcgctcgtg agagcgcgac cgggtgcgcc
      121 gacacggtgg ccgcggtgca cgcgccgttt caatccacgc gctcgtgaga gcgcgaccgc
      181 gctaccacgg cggcggtatc gccggcctgc agtttcaatc cacgcgctcg tgagagcgcg
      241 accggccgag tgggagtcga ccaacgaagt cctcctgttt caatccacgc gctcgtgaga
      301 gcgcgactag cgccgctcgt agcgcacgcc cttggtgaag atgtttcaat ccacgcgctc
      361 gtgagagcgc gaccacgccg ggcggccacg cgcatgagca tcaggtcgtt tcaatccacg
      421 cgctcgtgag agcgcgacca gtcgctggcc tagggtcgtg ttcatgctaa ggtttcaatc
      481 cacgcgctcg tgagagcgcg acctcgccgg cccgcatggc ggtctccaac gcaaagaggt
      541 ttcaatccac gcgctcgtga gagcgcgacc ggccccatcg ctccggggac gacttgggtc
      601 gaggtgtttc aatccacgcg ctcgtgagag cgcgactccc tcagcataaa tgcttggctg
      661 gacaggggaa aagactcatc aatgcgaacc gagctgacgg atacgcatct cgatcggttg
      721 caggggccac cacaggggtc ccatccatgc ggatcaatga cttagctgcg cgcgaaccgg
      781 cccggttgtt tccggtcgct tggggttcgc atcagaagat aagtggaccc ttgaagttca
      841 cgctgggctt ggcgccgatg tgttcaacgc ggccctccca cttcgcgccc agctgataaa
      901 agcgcaggct gtcgcactca gggtcgatca ggtcacacag tcgctgcctc agctggaccc
      961 actgcgcagg ttcgacctcg atctcaaaga ccgaatactg tacccgctga cccacgtcgc
     1021 ggcaggcctt ggcgacccgg cgcagtcgct tttctccgcc gggggcgatt gtgctcacgt
     1081 catagctgac caagaccatc atcgcgcatg gctccgtgtc attgccacag gaacggcggg
     1141 taaccgtcca agtccccacg caggtggcgt gccagcagtt gagcctgcac aaagggcagc
     1201 agtccaatgt ctactttctc gcccaggaac gcgtgttgca gctgttcgcg cttgcgctcc
     1261 tggtaggcca ccagcacgtt cttgcgggcc tcgtccttta gttgcactgc gccattgtcg
     1321 aagacctgaa agtcgcgttc attcagttgt cggcggttga tcagggacag cgcaaggcgt
     1381 tcgcccagca gtggcctgaa ttcttcggcc aggtcaagcg ccaagctggg gcgtcctggt
     1441 cggtcgcggt gcaggaagcc tactgcgggg tccaggccaa cggtctccaa tgcggagcgg
     1501 cagtcgtgcg tcagcaaggt gtagaggaaa gagagcaggg cgttcaaggc gtcgcgcgga
     1561 gggcggcggt tgcggccacc gaagcgcagc agcggcgatt cagcgcgcac gagctgatcg
     1621 aacacgccga agtaggcctg cgccgcttcg ccttccagcc ctcgcaggat atccacgtcg
     1681 ttctccagaa gaagacgctg cgggatgcgt cgcagccgct tgagcgcgtg ctgaaaagcg
     1741 ggtgcgtcag ccagccggtc gccgtggtcg cgccagccgc gcgccagcac ggcacgctgg
     1801 ttgtggatct tgccggccag cagattacga acgatggcgg cgcagcggga ggggtcgtcg
     1861 ctgcgccggt actgctcacg ccgaagcagt acgttgcccg atactggccc ctcgacccgt
     1921 gccaggaagc ggccctgtgg cgtcaggtag cagatgctga tgccctgtgc gctgcagaag
     1981 ccgagcaact gcggagatac cgccacgcgg ccgatgcaga ccaggctctc cagcatgtgc
     2041 acgggtaggc gcgcgcgctc ttggcgctca acttccagca cgatgttggc gccatccttg
     2101 cgcaaccagg caccgtcagt tgtggcgtaa agcgtgttga gctggcggcg catggctatt
     2161 cttcctcgtg ttcgagctgg cgctggagcc aggcttggac gctgcggcgg ccgagcagcc
     2221 gcggctggca caggtcgagc agcgagcagg ggtcgcagcg ttttgcctcg tagcgggccg
     2281 agggcgtttt ccccaacgcc agcatggcac gcgtgccggt gatggtggcc cgcgtcagtg
     2341 cacgcagttc ctggtcgaaa gtcacgtcct tgcggcgccg ggtctggccg tagaacaggg
     2401 cgcccgcgta gacgtgggtg tccagcatcg cttccagaca taacccttgg gcacacagct
     2461 gcacctcatc ggctcgatgt gacttggggc gcccgcgctt gtactcgacg ggaaagacgc
     2521 gttcgccgga ctcggtgtgg tggaactcga ccacgtcagc cttgccggtg atgcccagtt
     2581 gcaggttcag caggggcatc gcggtcacgg tgcgcacgcc gcgccgacgt tcggcctgcg
     2641 gaacgtcggc gcgctcgtgc aatagacggc cttcggccgt ctgccggttc tctttccact
     2701 gctgctccag atggatcagt gcacactggc gcggacagta aagataatgc tggagggcgg
     2761 agagcgggat caggtcttcg tcgtccatgg catccgctct cctccgcact tcagaacact
     2821 tcgatcaggg tcacgcccac cggcagttcg cgatcaatgt ggacccggta atcagcgaaa
     2881 ccgcgtgccg gcgtttcgga ctggccttcc acacgcgtca ccttgacctt gtcgaacagc
     2941 gcgtgcgccg gcgcgttgcc catcggatgc tcatgctcaa acgcgaccag cttgcgcgca
     3001 gccatctcgc cgcgtgccgc cgaccggtcg tgttcgaaca gattggtcag tgcgcgccac
     3061 aacagctgca gatcgtcctc ggagaaaccg gtgcgttcgg ccagcttggc cgagacgaag
     3121 ccgtgagcgc ggtaaaggcc gtaagggatg atgtgcttgc ggcccatcgt gcgattgtca
     3181 ccactctggg cttcggattc cttctcggtg gtgacagcca cacgtgtgat cgaaacttcc
     3241 agaggcaata ccggttccac cgatgttgca aaggcgagct gcaccgggcc gcgcacctgg
     3301 ccggcattga caccggtggt cattactgcg ccgaaggtcc ggacgtcaaa aaagtttcca
     3361 cacatccatg cggtcagttc gcgtgctttg gcttcgtcct tgggcagctt cttgaagcca
     3421 tccttgacat cgggcgcaat tccgagtgct tcccaggatt tctgatgctg ctggttgagg
     3481 attgcctttt cctacatgta gatcgcatag ccggcttcat tttccttttc cagcgcgacg
     3541 taattgcgga tctttcgctt gagcgccacg tcggtgacca ggccgcggtt ggtctccggg
     3601 tccaggcgtg gcaggttgcc ggcgtccggg tcgccattgg ggttgccatt ggtgacatcg
     3661 aacaagtaga cgaactcata gcggtgggcg atggcactca tgcggtttct ccttcggagt
     3721 cggtaagggg ggcgtcctgg ttgccgctgc gggcgaaacg cgcctgggtc tggtggtaat
     3781 agccgatggc gaagcggcct tgcgcttcga tcagcaggct acgtggaagg ctcggtggga
     3841 aggaatcgac gatctgaccg atttcctttt caaggctcat cgcgagcccg gccttgtcct
     3901 tgcgcaattt tccaaagtgg ttctgcgcgt tgcgcagcag caccgggaag atgcttgcag
     3961 gcgtagccga ggcagcgccg tagtaacggt cgcggatggt cgcgttgacc tgcgcgccca
     4021 gtgcggcgcg ctgcaggttt tcaagcgacg agaacagccg ccccagcagg tagccggggt
     4081 cggtattgct ggtatcaaga ctcacgggtg gttccccctt gttggttgac gttccatggt
     4141 gcatgcccag ccgcgcctcg cgcgccagga cggccttgca cagtgccacg cgcagtggac
     4201 tgatctgccc gtcggcgcgg aagcgcatca cgatcgcgcc aaggatgctg cgcgggtagc
     4261 gcatgccggt gaggatggcg cgcgtcagtt cgccggccag aagcggcggc acgtcctcgg
     4321 ccttgggcct gccgttctct ccatagaccg gtgcggtctg cagggccaga tactgcggcg
     4381 ttggcgggcg cctccaggcc gatggctcca gcgcgaggtc tcggtagtgg tcggccaggc
     4441 ggttggcgaa gcttgacagc gatgtcgttt cccagaatcg gatcgatagc cgcgaggcat
     4501 tcggcgccag gccaagcacg aaaatgcgcg tgtcctcgtc caggcggctg tccagttcgc
     4561 gcaagggccg caactgccgt agctgttcca gcgcctgggc caatcgccgg gttgcctggg
     4621 catcgtccag cccttcgctg tcgggttgct gtgtaccgaa gaaggtggca acgaaatcct
     4681 cggcagcctg ggcgcctgca ccctgtgccc agaacaccac ggtggtgtcc cccagctgca
     4741 ggcgctgatg attgtcctcg gtgcgccgca ggagatggtt gagcgcggtg gtataggcga
     4801 aagcagcctg ttcggacacc ggtgcgttct cgccctggct cttgccgtag gacgtgaacg
     4861 catccaggtt gaacgaaacg atggaggcac cggagctctg cgcgccattg acgcctttga
     4921 tggctgggtg caggcgggcc attggcgcct gttggccagt gacgagacac atgccgcgca
     4981 cgccgtccgc gcccagtgcc tgctgcctgt tccatgcatc gcgcgcggct tgtcgctggt
     5041 gcaggaactg ctgcgtgccg tcatcgaagc gaaagacgag gttggcatcg agcaaggcct
     5101 cgccatggcg agagaagaga ggattgtctg cgaactgggc cggcgtccag tcatcgagga
     5161 acgccagcag cgccttcagc ccagggtcct cactgccggc cagtgcctgc cggtgcaggg
     5221 ttttgaaggc ggcgtggtcc tgctgggtcc gtgccgtgtt tttcccgatc ccaagcacat
     5281 agctggtttt gtcccagagg aagttcgact tgaccgcgac tgtgcgtttt tcaggctgag
     5341 gcacgccaag taggcgcggc gccggcttct tgccgctgct gtcacgcagg tcctgcacat
     5401 cgaccacgcg gccgtccgcg tccagcacga tggcgtagcc gatcttctct tggctgtagc
     5461 ctggtgcggc gatgccggac tcgcggtcgt ccgacaggcg ccggtagtat tcgtcgagcg
     5521 cggacaggat catccgtgca ctccttcgtc gcctggcggc ggcacctgga tcaggccatc
     5581 gaccatgcgc gcccggaaaa aacgcggggt catgccgtcg gcgaagtcga tgtcgtgcaa
     5641 catccagccc aggtcgcgct cgccctgcag cgaggcatcc gcgatgggga ggtcgtagcc
     5701 gttctcgacc agggcgaagc tggcggggaa ctcgcgcgta cccaggcacg gtgcctggaa
     5761 gcattgcccg ctccgggccc tccggttgaa gatgtccaag tgcttgccga cgctgtcctc
     5821 cggcccggcc cgagcggtca gctcgaaatg cgcggcgatc acatagccca cgtcgcgcag
     5881 cagggtcgcc gcccgttgct ggcggtcctc ctcgacgtag ttgaccagtc cgtctgtccg
     5941 tccagccctg atcgccctgc caacgctggc ggcggacaac ttgcctccga cctcgttgcg
     6001 ccggatcgat tcgaatcgca cgggcctgag cacctggatg ccgtccacca cccagcggat
     6061 ggccggcttc caatggatcg cctccaggat gccgcgcgca gccgacggcg taatgacgtc
     6121 ataggagacg cgctcgacct tcatctcggg cctggtgaac agcgcgcgtt cgccccagac
     6181 gtgcagcttc accccgtaac tcatctcttg tccccctgtt tcatcgaaat cttcacccgc
     6241 cacagtcgca gggattgcga caggtgggca tgtcgtattt ttgggttgtc tcacacaatc
     6301 ccatcggaaa atccgctgat gcctcgcaga cctgccacga gcatggccgc ggccgaggtc
     6361 tcatctccgc atccggtgcg gaaagaaaaa agaggagcga atcatccgac gagcgcttcc
     6421 gccgccaaga actggggatt gtcgtcccaa tgcagtccga atttcgggtg gtacaggcgc
     6481 gggttgacca gccggacgaa ctgctcgcca tagcgctccg gagccaccgg ctggatcgca
     6541 ccggcattcc acagcgcgtc gtagccttgc cggggcacct gtaccaggaa tggctgcagc
     6601 ttgcgggccg tgcccggagc atcttcgaga tcgcgcagcg cgctggccac gaaggggtct
     6661 tccgcgccgg tctgcgggtc gtagggcacg atcactggtc gcatgttggc ttcgatcatc
     6721 cggaaccggg ctgccaaggt ttccagcggc agatcgtcga cggtggcgcc atcgaacaac
     6781 tgcagcagca tctcgccaag ctggccgtcc ctgcgccagt acagctcgcg aaagtagtcg
     6841 gtcacggctt caagcgacaa caggtcggca cggtgccggc gttcgatcat acgaaacgcc
     6901 tctgcgaaca acttcagctc cttcggtggc gcccaatccg ggctttcgaa caccaggacc
     6961 tggctgtcct ccaccgccca ctccatgttg cgattgcagc ggccggcagc ctgggcgatc
     7021 gagtccaggc cggcctcggc gcgcaacacg gccgggaacg aaacgtccac tcccgcttcg
     7081 atcaggctgg tcgacaccag ccggcatggt tggcctgcct tcaaccgggt ccgcaggtcc
     7141 tgcagtacct ggctgcggtg gcgcgcgtac atcagcgtgg tcagatggca tgcacctgga
     7201 aggtcggcga tggcatcgaa caatgcgcgc gcgtggcggc gattgttgac gatgcagagg
     7261 atctgttccc ggctgcgcag gtggccggct agcgcggcgt cgtccagcgg gccggcatgc
     7321 tgcacgctta cccgcttgag ccgccggtac agccccggtg gatccggcgc cagttcgcgc
     7381 acgccttcca ggccgtcctt gaagccatcg gccttgccca gcgccggctg ggtcgcggtg
     7441 cagagcatca tgctgacacg gtagttgagc gccagttcgt ccagtgcctg catgcacggg
     7501 cgaagcagat gcaacggcaa ggtctgtgcc tcgtccagga tcaccacgct tgcagcgatg
     7561 ttgtgcagct tgcggcagcg tgcgggacgg tcggcgaaca ggctttcgaa gaactgcacg
     7621 gcggtggtga cgatgatcgg cgcgtcccag ttctccatcg ccagatggcg tttttccttc
     7681 gattgcagct ccgacttcgg atcgtcgaag aaagcgctgt ggtgctccag tacggcatcc
     7741 tcgcccagtt cactaaacgc gctgcggaac acggcggcgg tctgctcgat gatgcttgtg
     7801 aacggcacca cgtagatcac ccggcgcagg ccgtgggcga tggcgtggtc caaggcgaag
     7861 gcgagcgagg ccagggtctt gccgccgccg gtgggcacgg tcagcgagaa tagcccgggc
     7921 ttgtgcgtgg cctgggcgcg gacgtgtttc aggatatccg cccgtagcgg attgatgccg
     7981 ccctgtcggg gcaggctgac cagatgggca tccagctttg cgcgcaggac gtccagggac
     8041 gggccggtgc gctctgggcg gggatcgcgg ccctcgatgc gccggtagaa gttgtcggta
     8101 tcgacgaagt cggcatccac caggcaggag aagaccatac gcgccagcag cgagaactgg
     8161 aatgccggcc tggtcgacac cgaactgaac cgggtgggca gcgccagccg ttgcggcagc
     8221 tcgagctcgt gttcccactc cggcagcaac ggcggcaatt cgcggagcag gtagtcctcg
     8281 gacagccgtt cgcgcagaca ggaccgagct tcgccaaggg tgccgttggc caggccggca
     8341 tgatggcccg cgatggcgta ggccatcagt gtgcccagcc ccttgtagcg ctgctgcgcg
     8401 attcgggcac cccaagtggc atggtccacg cgtgggacct cgcctctcag ccgatcctgg
     8461 aatcgccggg tgtacttgcc cagatcatgc agtgcacccg cgatccagcc cagttcctgt
     8521 ccgccgaatg gcgcagcatt ggccgaggcc cgctccgcaa cgcccaacaa atgcgcccgc
     8581 aacccctgcc aatccgagca gtctttccgg tcggtcgaat gcgcgtagaa ctccatttcc
     8641 ttccccctgt ggcgagacca accctgctgt cccgatgatg tgcaagctta tctagcccga
     8701 tctcatggag tgagaagggg tgaccacagg acccaggttg ggcatgctgg caggccgcct
     8761 ggacccgtga gctcccgcat gtccgatccc gaaccccgca tccagcttga gccgtcgtgg
     8821 aaggcgcggg tgggcgattg gctgctgcgg ccggagatgc agcagctgtc ggcgttcctg
     8881 cgccagcgca aggcggcggg cgtgcacgtg tttccgccgg ggccgcagat cttcgcggcc
     8941 ttcgaggcca cgcccttcga caaggtcaag gtggtgatcc tgggccagga tccctaccac
     9001 ggcgccggcc aggcgcacgg gctgtgcttc tcggtgccgc cgggcgtgcc ggtgccgcca
     9061 tcgctgcaga acatctacaa ggaactggcc agcgatgtcg gggttgcgcc gccagaccat
     9121 ggctgcctga gcccgtgggc gcggcgtggc gtgctgctgc tcaacgcggt gctgacggtc
     9181 gaggagggcc gccccggcgc gcaccggggc aagggctggg agggcttcac cgaccaggtg
     9241 atccaggccc tggcggccga gcgcgaacac ctggtgttcc tgttgtgggg cagctatgcg
     9301 caggccaagg gcaaggtcat cgatcccgcc cggcaccggc tgctcaaggc gccgcatccc
     9361 tcgccgctgt cggcgcatcg cgggttcctg ggctgccggc atttctccca ggccaaccag
     9421 tacctggaac gccacgggct gggcgcggtg gactggacgc tgccgcggcg cgcgcagctg
     9481 gaagcctgac cgccggtcag ggctccttct ggggggtggt cgtcttggtg gccgtgcctg
     9541 acttgctgga cgaggacttc ttgctgctgg tggacttctt tctggtgctg ctggtcgccg
     9601 gcttcttgcg cgcttccggc ggcgataccg gcggggccgg gagcggctcg gccgagacgg
     9661 tcttgacctc ggcctgcagg gcttccagct gcgggatcat cttctgctgg atggccacca
     9721 tcagggtctg catcatcacc ggggtccggt cgagcaggtg ctggccggcc ctggactcgt
     9781 agaacctggt catcgccttg acgtcgtcgt cgtcgaaggt cttgcggtac acgtccaggt
     9841 acaccgggcg catcgattcc caggccagcg cctggcgcac gatgttggag gtcttcagct
     9901 ggatctgctg gatctcggcc tgctgttcgg ggctgagggt cttgccctgg gtcagctggg
     9961 cgaactgctc gcgctgcatg gcttccacct gcggctggat gcccgccagc atgctctgcg
    10021 cgcgcgaggc ggtgagcagg cgctcgatct cctcgtcggt gggttcgcgg gccagcgcgg
    10081 gcggggcgat cgcagccatc agggacatca gcagcaggac aggtgcgaat cggggatatg
    10141 gcatgcgcag ctcgagcgtg aagcgaggca ggcgatggtg ccgaggtggc gcgtgcgccg
    10201 tgtaaaaatt tcactaaatg gtcgcagtgg tcagccgtgg gtagccacgc cgatccccta
    10261 caatgccggc ccatggccgc cggctccctg cagatcacgc cccagctgac gatccccgac
    10321 gacgaactgg tcgagcgttt cgtgcgcgcc agcggtgcgg gcggacagaa cgtcgacaag
    10381 gtcgccaccg cggtggagct gcgcttcgac gtggccggct cgccctcgct gcccgagctg
    10441 ctgcgcagcc gcctgctggc ccggcgcgac cgcaggctca ccgatgaagg cgtgctggtg
    10501 atcgaggccc agcgcttccg cacccaggac cgcaaccgcc aggatgcacg cgagcggctg
    10561 gcgcagatca tcgcccaggg cctgtcggtg cccaagccgc gggtcgccac ccggcccacg
    10621 cgggcctcca ggctgcgccg gctcgatgcc aagaagaccc gcagccaggt caagcgcaac
    10681 cggaacaccc gtgactggga ctgagcccac gccacggccg cccggccaga tcccggttgc
    10741 cccgcccaac atgccccggg tgaagcccaa ccgcttcacc cgctggtgcg ggcgcagcct
    10801 gctgcgcctg tgcggctggc gcatcgccgg cgagctgccc gacgtgccca agctggtgat
    10861 gatcgtggcg ccgcactcgt ccaactggga cgggctgtgg ggcatggcgg tcaagatcgc
    10921 ctgcggcttc gaggtcaagg tgctgggcaa ggcccagctg ttctggtggc cgctgggacc
    10981 gctgctgcgc cgcctgggcg cgatcccggt ggaccgcgcc aacgccggtg gcgtggtgcg
    11041 ccaggcggtg gacgccattc gcgccgcccc gcgcatctgg ttcgtggtca ccccggaagg
    11101 aacgcgcaag cgcgtggaga agtggaagag cggcttctac aagatcgccg tcagcgccca
    11161 ggtgccgatc ctgctggcct acttccacta cccggagaaa atcgtcgggc tggggccgtt
    11221 gttccatccc accggcgatg ccgaggccga catggccgcg atccgcgcct ggtatgcccc
    11281 gtggatgggc aaacaccgcg gcagcacctg atcggttgcg cggcgcgcgt tgcgcgtctt
    11341 catggcacgc gcgccctccg ctgttgtgca cgaacagggc gcaagccttg ttccgcgcct
    11401 tgccgcctgg cgcacgcgca cgtagatttc cgggtgcagg ccgtgcagct cgtcagcgca
    11461 tgcccgcacc atggccgcgg cgaaggccag caagacaccg ctgccggtgc agcccccggc
    11521 cgaccaaagc ccgacaggct gctagtgtag tgttccattc tgtttggaac ttaagcgctg
    11581 attggcgcgg atcaccttct gcaggatgtc gcgggcgctt ctggtccaga tgaacggctt
    11641 gggtttggtg ttgtgatgcg cgaggtactc gtcaatggcg g
//
LOCUS       DATCUN010000282         7214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_51431, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000282 DATCUN010000000
VERSION     DATCUN010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51431"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /gene="metR"
                     /locus_tag="VIO83_10920"
     CDS             <1..515
                     /gene="metR"
                     /locus_tag="VIO83_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator MetR"
                     /protein_id="HEY8331823.1"
                     /translation="LARGDLDLVVTSDPVELAGITYVPLFTYEAQLAVANQHPLASKP
                     YVTPEDLAGETLIHYPVERDRLDIFTRFLDPADVEPAGVRTAELTVMMLQLVASGRGV
                     SCLPNWALHEYSSRGYVTAKKLGEKGLYATLYAGIRADMLDAPFMRDFLLTAKDTSFA
                     TLEGVSAARG"
     gene            complement(689..1600)
                     /locus_tag="VIO83_10925"
     CDS             complement(689..1600)
                     /locus_tag="VIO83_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEY8331824.1"
                     /translation="MAGKLHWLGVIRGRFLLLALTCVALGIAAALKTRDLDGGFVVDA
                     LLVLLGALAAHAGVNALNEYSDYRSGLDLQTRRTPFSGGSGTLVAQPQYVEVAQWLGL
                     GAVGLTTAIGLYFLVRQPALVWSLAPLGAVGLALVLAYTPWLTRRPWLCLLAPGLGFG
                     PLMVLGTQVVLAGQISLSAALLSLVPFFLCNNLLLLNQFPDIEADRSVGRLTLPMLLG
                     RAGAVQVLCVQWLLAFAGLLVCVLAGWLPAGAAAGLLGALPALRAWRLLRRDADDLEC
                     LLPAMGLNVAVSVLTPLLMAVGMLVAG"
     gene            1799..4954
                     /locus_tag="VIO83_10930"
     CDS             1799..4954
                     /locus_tag="VIO83_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015636847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY8331825.1"
                     /translation="MDTDIMGAPEQTSSAADQSVRPPALQASLKTSRRRIWAVRGGFA
                     VVLLGCAAAGALWAAREAQTSEWQSRLLADWSARLQYRVEPGPSDAIRYPQQGPFDQR
                     LGYVGLPEFIRRLNNQGYVVASQARFSPPLLEYTDHGLYPPFPEKTRAGIDISDCRGE
                     PMFDFRHPQRFYESFNDIPPLAVQSLLFIENRNLLDPERPHLNPSVDWPRFLKAAWSQ
                     LARRLDADTDSAGGSTLATQIEKFRHSPEGRTPNAAEKLRQMLSASVRAYREGRETLP
                     LRREIVHDYINNVPLSAAGGYGEVHGIGDGLWVWFGADFARSNRALRSNSDASLAERG
                     LALRQVLALMIAQRRPSWYLNNGRAELAELTDSHLRVLAGAGVISPALRDAALGQQLT
                     FRNMATDPALPPVASDKGINAVRTRLVRQLGTQLYDLDRLDLSVDATLNQLLQQEVSN
                     YLGKLTDPVFAEQIGLFGERLLSPALTADVRYSFTLFERGANGNRVRVQTDTTGQPFD
                     INEGSKLELGSTAKLRVMATYLEMVSELHDHYAGKTPAELRSLAEHVNPRDNLSVWAL
                     DYLRVNPQASVEAMIDAALERKYSASPGETFFTGGGAHTFNNFRKEDNGRRPTMREAL
                     RESINLPFVRLLRDVVRYSTYNMANNSAQLLTDDQDPRRREYLTRFADKEGTVFLRRF
                     WSKYRDKPPQEMFDTLLEGLRLSQSRLAAIHRYLYPQASLEEFAQVMQARLGKKLMPP
                     PKKLEDYYHRYGPGAFNLPDQGYIARVHPLELWLIGYRLQNPQADFSAVAEASRDERQ
                     EVYGWLFKTRHRSARDSRIRTMVEVEAFTDVHQRWARLGFPFDHMVPSLASALGSSGD
                     RPAALAELMGIILNNGVRLPTVRIDNLHFAATTPYETRMERLQTSGERVMRPEVATAL
                     RGVLAEVVENGTARRLKGVLLQADGQPLMVGGKTGTGDNRLETFTRSGWVSSSTARNR
                     TATFVFFLGPRHFGTLTAYVAGEQSEKFTFTSALPVQTLKGMMPLLQSYLLAEATACQ
                     APEKGAAAEPQMHAALSPLKE"
     gene            4958..6772
                     /locus_tag="VIO83_10935"
     CDS             4958..6772
                     /locus_tag="VIO83_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEY8331826.1"
                     /translation="MIPRPPRQLRCMLGLSVSLLLAACTRDQALPLEIRPIQPEAASG
                     HQAKPGWQLQHYAVAAANPLAAEAGRQILQAGGSAVDAAIAVQMVLGLVEPQSSGLGG
                     GAFLLHWDGQRLDAWDGRETAPADADEQMFLQSDGKPMAFIDAVVGGRAVGVPGVLKM
                     LESAHRAHGKLPWAVLFVPAIELAEQGFAISPRLHQLLAADPYLRQDPSAASFYYRAD
                     GSALPVGQVLRNPALAEMLRRIARHGSTAFYAGPVAQAMVAKVRQHPSNPGRLQLADL
                     QHYQPLRREALCQLWLAQYRVCGFPPPSSGQLTLMQILGLLEQLPPQENPLHDGRPSA
                     AFLHRYSEAAKLAFADRAQYIADPAFVAPPAGDWRSLLAPAYLQQRATLIGERSMGSA
                     SHGVPGGTPLAWAPQAAQEEYGTSHVSIVDAEGRALAMTSSVEQAFGARQLTDGGTAL
                     PGGFLLNNQLTDFALAPRDAQGRPVANRIEPGKRPRSSMSPTLVFDAKNGELLASLGA
                     PGGAAIIHFTAKTLLGLYPWQLDAQRAIDLPNFANFNGPTVLEQGRFPPATLEALRQQ
                     GHEVGESEMPSGLQAIQRTPEGWFGGADPRREGVVLGE"
     gene            complement(6777..7001)
                     /locus_tag="VIO83_10940"
     CDS             complement(6777..7001)
                     /locus_tag="VIO83_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HEY8331827.1"
                     /translation="MTQPQKNPLHGITLEALLTALVAQYGWDGLAQRVDIRCFKSDPS
                     IKSSLTFLRRTPWAREKVEALYMQLRQQQG"
BASE COUNT         1208 a         2675 c         2346 g          985 t
ORIGIN      
        1 cgctggcgcg cggcgatctc gacctggtgg tcacctccga cccggtcgag ctggccggca
       61 tcacctacgt gccgctgttc acctacgagg cgcagctggc ggtggccaac cagcacccgc
      121 tggcgagcaa gccctacgtc accccggagg atctggccgg cgagacgctg atccactacc
      181 cggtggaacg cgaccgcctg gacatcttca cccgtttcct cgacccggcc gacgtcgagc
      241 cggccggcgt gcgcaccgcc gagctgacgg tgatgatgct gcagctggtg gccagcggcc
      301 gcggggtgag ctgcctgccc aactgggcgc tgcacgaata cagctcgcgc ggctacgtga
      361 cggcgaagaa gctcggcgaa aagggcctgt acgcgaccct ctacgccggc atccgcgccg
      421 acatgctgga tgcgcccttc atgcgcgact tcctgctcac cgccaaggac acctctttcg
      481 ccaccctgga aggagtcagc gcggcacggg gctgaacccc acgtcgcgcc aggcgcaggg
      541 ttacatcccc agtgccttcg gcggccaggt cgaaatccat cggcatgtgc tggcgcgacg
      601 agccgcacca ggacagcacc accctcgacc tgctggacag cctgcgccag gcggcgacca
      661 caccgatccg cgccgctgga gtgagcggct agcctgccac cagcatcccc accgccatca
      721 gcagcggcgt caacacgctc accgccacgt tcaagcccat cgccggcagc aggcattcca
      781 ggtcgtcggc atcgcggcgc agcagccgcc aggcgcgcag ggccggcagg gcgccgagca
      841 ggccggcagc ggcgccagca ggcagccaac cggccagcac gcagaccagc aggccagcga
      901 aggccagcag ccactgcacg cagagcacct gcaccgcacc agcacggccg agcagcatgg
      961 gcagggtcag acggccgacc gagcggtctg cttcgatgtc ggggaactgg ttgagcagca
     1021 gcaggttgtt gcacaggaag aacggcacca gcgacagcag tgcagcgctg aggctgatct
     1081 gcccggcgag gacgacctgg gtgccgagca ccatcagcgg gccgaagccg aggcccggcg
     1141 ccagcaggca cagccagggg cggcgggtca gccagggggt gtaggcaagc accagcgcca
     1201 gacccacggc gccgagcggg gccagactcc agaccagtgc cggttggcgg accaggaagt
     1261 acaggccgat ggcggttgtc agccccaccg cgccaaggcc cagccactgc gcaacctcga
     1321 cgtattgcgg ctgggccacc agggtaccgc tgccgccgct gaacggggtg cgccgggtct
     1381 gcaggtcgag gccgctgcga tagtcgctgt attcgttgag ggcattgacc ccggcgtggg
     1441 cggccagggc accgagcagt accagcaggg cgtcgaccac gaagccgccg tccagatcac
     1501 gcgttttcag cgccgcggcg atgccgagcg ccacgcaggt cagcgccagc agcagaaagc
     1561 gcccgcgaat cacccccagc cagtgcagtt tgcccgccat ccctgtccct cccggtttca
     1621 gattgctgca taatccgcgc ccgcgtcaca gacggagcgt cagtcatttc cctcggtcgc
     1681 agcgcctgca tgtggtgctc cgaactctgg gccggcatga tatcgctctg tgtgacagct
     1741 cgcaggcgac cgctggtgaa tacggctaag ctgttgtcaa caatcgataa atgccgtcat
     1801 ggatacagac atcatgggcg cccccgagca gacctcatcc gccgccgatc aatccgtgcg
     1861 cccgcccgcc ctgcaggcct cgctgaaaac cagtcgacgc cggatatggg ccgtgcgcgg
     1921 gggattcgcc gtggtcctgc ttggctgcgc cgccgccggc gccctgtggg cggcgcgcga
     1981 agcgcagacc tcggagtggc agtcgcgtct gctggcggac tggtcggcac gcctgcagta
     2041 ccgtgtcgag cctggcccca gcgatgccat ccgctatccg cagcagggcc cgttcgacca
     2101 gcgcctcggc tatgttggcc tgccggagtt catccgccgc ctgaacaatc agggttacgt
     2161 ggtcgccagc caggcgcggt tttccccacc gctgctcgag tacaccgatc acggcctgta
     2221 tccgccattc cccgagaaga cccgcgcggg catcgacatc agcgactgcc gcggcgagcc
     2281 gatgttcgac ttccgccacc cgcagcgctt ctacgaaagc ttcaacgaca ttccgccgct
     2341 ggccgtgcag agcctgctgt tcatcgagaa ccgcaacctc ctcgacccgg agcgcccgca
     2401 cctcaatccc tcggtcgact ggccgcgctt cctcaaggcg gcctggagcc aactggcccg
     2461 ccggctggac gccgataccg actccgccgg cggcagcacc ctggccacgc agatcgagaa
     2521 gttccgccac tcgccggaag ggcgcacgcc caacgcggcg gaaaagctgc ggcagatgct
     2581 ctccgccagt gtgcgcgcct accgcgaagg gcgtgaaacc ctgccgctgc gccgggagat
     2641 cgtccacgac tacatcaaca acgtgccgct ctccgcggcg ggcggttatg gcgaggtgca
     2701 tggcatcggc gacggcctgt gggtgtggtt cggcgccgac tttgcccgca gcaaccgggc
     2761 gctgcgcagc aactcggatg caagcctggc cgagcgcggc ctggccctgc gccaggtgct
     2821 ggcgctgatg atcgcccagc gccggccgtc ctggtacctg aacaacggcc gcgccgaact
     2881 ggccgagctg accgacagcc acctgcgtgt gctggccggc gcgggggtga tcagcccggc
     2941 cctgcgcgat gccgcgctcg gccagcagct gacgttccgc aacatggcca ccgatccggc
     3001 actgccaccg gtagccagcg acaagggcat caacgccgtc cgcacccggc tggtgcgcca
     3061 gctcggcacg cagctgtatg acctcgaccg cctcgatctc tcggtcgacg ccaccctcaa
     3121 tcagctcctg cagcaggagg tcagcaacta cctgggcaag ctgaccgacc cggtcttcgc
     3181 cgagcagatc ggcctgttcg gcgagcgcct gctgagcccc gcgctgaccg cggatgtgcg
     3241 ctacagcttc accctgttcg agcgcggcgc caacggcaac cgcgtgcgcg tgcagaccga
     3301 caccaccggc cagccgttcg acatcaacga gggcagcaag ctggagctgg gctccaccgc
     3361 caagctgcgg gtgatggcca cctatctgga aatggtcagc gaactgcacg accactacgc
     3421 cggcaagaca ccggccgagc tgcgcagcct ggccgagcat gtcaaccccc gcgacaacct
     3481 cagcgtgtgg gcactggact acctgcgggt caatccgcag gccagcgtgg aagcaatgat
     3541 cgacgccgcg ctggagcgca aatactccgc cagccccggc gaaaccttct tcaccggcgg
     3601 cggcgcacac accttcaaca acttccgcaa ggaagacaac ggccgccgac cgaccatgcg
     3661 cgaagcgctg cgcgagtcga tcaacctgcc gttcgtgcgc ctgctgcgcg acgtggtgcg
     3721 ctacagcacc tacaacatgg ccaacaacag cgcgcagctg ctgaccgatg accaggatcc
     3781 gcgccgccgc gagtacctga cccgcttcgc cgacaaggaa ggtacggtgt tcctgcggcg
     3841 cttctggagc aagtaccgcg acaagccccc gcaggagatg ttcgacaccc tgctcgaggg
     3901 cctgcgcctg agccagtcgc gcctggccgc catccatcgc tacctgtacc cgcaggccag
     3961 cctggaagag ttcgcccaag tcatgcaggc ccgcctcggc aaaaagctga tgccgccgcc
     4021 gaagaaactc gaggactact accatcgcta cggtccgggc gccttcaacc tgcccgacca
     4081 gggctacatc gcccgcgtgc acccgctgga gctgtggctg atcggctatc gcctgcagaa
     4141 tccgcaggcc gacttcagcg ccgtggccga ggccagccgc gacgagcgcc aggaggtcta
     4201 cggctggctg ttcaagaccc ggcaccgcag cgcccgcgac agccgcatcc gcactatggt
     4261 cgaggtcgag gccttcaccg acgtgcacca gcgctgggcc cgcctgggct tcccgttcga
     4321 ccacatggtg ccgtcgctgg ccagcgcgct gggcagttcc ggcgaccgcc cggcggcgct
     4381 ggccgagctg atgggcatca tcctcaacaa cggcgtgcgc ctgccgacgg tacgtatcga
     4441 caacctgcac ttcgccgcca ccacgcccta cgaaacccgc atggagcgcc tgcagaccag
     4501 cggcgagcgg gtgatgcgcc ccgaagtggc cacggcgctg cgcggcgtgc tcgccgaagt
     4561 ggtggaaaac ggcaccgcgc gccgcctcaa gggcgtactg ctgcaagctg atggccagcc
     4621 gctgatggtc ggcggcaaga ccggcaccgg cgacaaccgc ctggagacct ttacccgcag
     4681 cggctgggtg agcagttcga ccgcgcgcaa tcgcactgcc accttcgtgt tcttcctcgg
     4741 cccgcggcac ttcggtacgc tgaccgccta tgtcgccggc gagcagtcgg agaagttcac
     4801 cttcacctcg gcgctgccgg tgcaaacgct caagggcatg atgccgctgc tgcagtccta
     4861 cctgctggcc gaagccaccg cctgccaggc gcccgagaag ggagccgcgg cggagccgca
     4921 gatgcacgcc gcgctgagtc cgttaaagga ataagccgtg atcccgcgtc cccctcggca
     4981 gctgcgctgc atgctcggcc tgagcgtcag cctgctgctc gccgcctgca cccgcgacca
     5041 ggcgctcccc ctggagatcc gcccgatcca gccggaggcc gccagcggcc accaggccaa
     5101 gcccggctgg cagctgcagc actacgcagt ggccgccgcc aacccactgg ccgccgaggc
     5161 cggccggcag atcctgcaag ccggcggcag tgccgtggac gcggcgatcg ccgtgcagat
     5221 ggtgctgggc ctggtcgagc cgcagtccag cggcctcggc ggcggcgcgt tcctcctgca
     5281 ttgggacggc cagcgcctgg atgcctggga cggccgcgaa accgcgccgg ccgacgctga
     5341 cgagcagatg ttcctgcaaa gcgacggcaa gccgatggcc ttcatcgacg cggtcgtcgg
     5401 cggccgcgcg gtaggcgtgc ccggcgtgct gaagatgctc gagagcgccc atcgcgccca
     5461 tggcaagctg ccctgggcgg tgctgttcgt ccccgccatc gagctggccg aacagggctt
     5521 cgccatcagc ccgcgcctgc accagctgct ggccgccgac ccctacctgc gccaggaccc
     5581 gagcgccgcc agcttctact accgggccga tggttcggcc ctgccagtcg gccaggtgct
     5641 gcgcaacccg gcgctggccg agatgctcag gcgcatcgcc cgccacggca gcacggcctt
     5701 ctacgcgggc ccggtggccc aggccatggt ggccaaggtc cgccagcacc cgagcaaccc
     5761 cggacgcctg caactggccg acctgcagca ctaccagccg ctgcggcgcg aggccctgtg
     5821 ccagctgtgg ctggcccagt accgggtctg cggcttcccg ccgccctcct ccgggcagct
     5881 gacgctgatg cagattctcg gcctgctcga acagctgccg ccgcaggaaa acccgctgca
     5941 cgacggccgc ccctccgcgg ccttcctgca ccgctacagc gaagcggcca agctggcctt
     6001 tgccgatcgc gcgcagtaca tcgccgaccc ggccttcgtc gcgccgccgg ccggcgactg
     6061 gcgcagcctg ctcgccccgg cctatctgca gcagcgcgcc acgttgatcg gcgagcggag
     6121 catgggctcc gccagccatg gcgtccccgg cggcacgccg ctggcctggg cgccgcaggc
     6181 ggcgcaggag gaatacggca ccagccacgt cagcatcgtc gatgccgaag gccgtgcgct
     6241 ggcgatgacc agcagcgtcg agcaggcctt cggcgcgcgc cagctcaccg acggcggcac
     6301 cgcgctgccc ggcggcttcc tgctcaacaa tcagctcacc gacttcgccc tcgccccacg
     6361 cgatgcgcag ggtcggccgg tcgccaaccg catcgagccg ggcaagcggc cgcgctcgag
     6421 catgagcccg accctggtgt tcgatgccaa gaacggcgaa ctgctggcca gcctcggcgc
     6481 gcccggcggc gcggcgatca tccacttcac cgccaagacc ctgctcggcc tgtacccgtg
     6541 gcagctcgac gcccagcgcg ccatcgacct gcccaacttc gccaacttca acggccctac
     6601 cgtgctggaa caggggcgct tcccgccagc cacgctcgaa gcgctgcgcc agcagggtca
     6661 cgaagtcggc gagagcgaga tgcccagcgg cctgcaggcc atccagcgca cgccggaggg
     6721 ctggttcggc ggcgccgatc cgcgccgcga gggagtggtg ctgggcgaat agggcctcag
     6781 ccctgttgct ggcgcaactg catgtacaag gcttccacct tctcccgcgc ccagggcgtg
     6841 cggcgcagga aggtcaggct cgacttgatg ctcgggtcgc tcttgaagca gcggatatcg
     6901 acgcgctgcg ccaggccgtc ccagccatac tgcgccacca gcgcggtgag cagggcctcg
     6961 agggtgatgc catgcaacgg gttcttctgc ggttgggtca tgatcggcct ggccaagtgg
     7021 atatcgggca aagaaggcgc gcagcataac ccagcccccg cgctcgcgag gagccatcgc
     7081 gggcgtggac gcccccttca gcggaccagc agcacgcccg ccgccgctgc cgccaccagc
     7141 aggccaccgg ccagcaacgc cagcttgagc aggttggagt cggccttcgc ggcagggtgc
     7201 gccgccgttt cggc
//
LOCUS       DATCUN010000283         3316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_69506, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000283 DATCUN010000000
VERSION     DATCUN010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69506"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VIO83_10945"
     CDS             <1..327
                     /locus_tag="VIO83_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8331828.1"
                     /translation="DDGRLKWDGVLIGASAGTRVRAIHGGRVVFADWLRGAGLLLIVD
                     HGNGYLSLYGHNQSLLKAAGDLVKAGEAISTVGSSGGQDTPALYFAIRQQGQPSDPLR
                     WCRAQG"
     gene            354..1679
                     /locus_tag="VIO83_10950"
     CDS             354..1679
                     /locus_tag="VIO83_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY8331829.1"
                     /translation="MSSRFRLSALALSLSLALLGAAPLVQAAESADGTSPAPPAGKAT
                     LPLDELRTFAEVLERIKSAYVEPIDDKTLLENAIKGMLSNLDPHSAYLEPEDFKELQE
                     STSGEFGGLGIEVGQEDGLLKVVSPIDDTPAARAGIEAGDLIVKIDGQPTKGWTLVQA
                     VDKLRGKAGSKIVLTLVREGGKPFDVKLERAVIKVRSVKSELLEKNYGYLRITQFQVN
                     SGEEVAKALATLKKDNGGKLGGLVLDLRNNPGGVLQAAVEVSDHFLSKGLIVYTKGRI
                     ANSELRFSADRADASEGVPLVVLINGGSASAAEIVAGALQDHKRGVLMGTDSFGKGSV
                     QTVLPLTNERALKLTTALYFTPNGRSIQAQGIVPDIVVERGKVTHDDGPSQGLKEADL
                     SGHLGNGNGGADKPSNGKPAADTSKPQDEDYQLSQALNLLKGLNITRQP"
     gene            1854..2213
                     /gene="tnpB"
                     /locus_tag="VIO83_10955"
     CDS             1854..2213
                     /gene="tnpB"
                     /locus_tag="VIO83_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449170.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEY8331830.1"
                     /translation="MMRPNAKVSKVYLYPKPVDFRKSIDGLAALVELDIKVAVFDPVL
                     FVFLNRARNRVKILYWERNGFCLWLKRLEAERFKAPPEPGDEAIVLSARELNRLLDGF
                     NLWGNRPHKILTPLYVA"
     gene            2232..>3316
                     /locus_tag="VIO83_10960"
     CDS             2232..>3316
                     /locus_tag="VIO83_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010954007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEY8331831.1"
                     /translation="MISVPDTLPDDPAALKQLLLALSEQVAVKDGQILQLREENILLR
                     QRLFGRKSEQTADPATPQLALFNEADSLAEPLDEAADNEVVEEVVVPSAKRRGKRKPL
                     PAELPRVEVIHELPEHELACECGCRKHVSGEEVSEQLEIVPMQIRVLRHIRKVYACKG
                     CESAPVTADKPAQLIEKSLASPSVLAMLLTTKYVDGLPLYRFEKVLARHGIDLPRQTL
                     ARWVIQCGELLQPLLNLLREHLLASPMIHCDETRVQVLKEAGREPSSQSWMWVQTGGP
                     PDRPVVLFDYTTSRAQEVPLRLLEGYSGYLMTDDYAGYNALAARPGIERLACLAHARR
                     KFVEAQKVQPKGKTGRADVALNLINRL"
BASE COUNT          625 a         1120 c         1084 g          487 t
ORIGIN      
        1 gacgacggcc gcctgaaatg ggacggcgtg ctgatcggcg ccagcgccgg cacccgggtg
       61 cgcgccatcc acggcggccg cgtggtgttc gccgactggc tgcgcggtgc cggcctgctg
      121 ctgatcgtcg atcacggcaa cggctatctc agcctgtacg gccataatca gagtttgttg
      181 aaggccgccg gcgacctggt caaggccggc gaagccatct ccaccgttgg cagcagcggt
      241 ggccaggata ccccggcgct gtacttcgcc atccgccagc agggccagcc cagcgatccg
      301 ctgcgctggt gccgcgccca aggctgaccg cacgcccatt caggagtcac cccatgtcgt
      361 cgcgttttcg tctctccgcc ctggccctgt ccctgtccct cgcgctgctc ggcgccgcgc
      421 cgctggtgca ggccgcggag tcggccgatg gaaccagccc cgctccgccc gcgggcaagg
      481 ccaccctgcc gctggacgag ctgcgcacct tcgccgaagt gctcgagcgc atcaagagcg
      541 cctatgtcga gccgatcgac gacaagaccc tgctggaaaa cgccatcaag ggcatgctca
      601 gcaacctcga tccgcattcg gcctacctcg agccggagga cttcaaggag ctgcaggaaa
      661 gcaccagcgg cgagttcggc ggcctcggca tcgaagtcgg ccaggaggat ggcctgctca
      721 aggtggtctc gccgatcgac gacaccccgg cggcacgcgc cggcatcgag gcgggcgacc
      781 tgatcgtcaa gatcgacggc cagccgacca agggctggac cctggtgcag gcggtggaca
      841 agctgcgcgg caaggccggc agcaagatcg tgctgaccct ggtccgcgaa ggcggcaagc
      901 ccttcgacgt caagctcgag cgcgcggtga tcaaggtgcg cagcgtgaag agcgagctgc
      961 tggaaaagaa ctacggctac ctgcgcatca cccagttcca ggtcaacagc ggcgaggaag
     1021 tcgccaaggc gctggccacg ctgaagaagg acaacggcgg caagctcggc ggcctggtcc
     1081 tcgacctgcg caacaacccc ggcggcgtgc tgcaggcggc ggtggaggtc tccgaccact
     1141 tcctgagcaa gggcctgatc gtctacacca aggggcgcat cgccaactcc gagctgcgct
     1201 tctccgccga ccgcgccgac gccagcgaag gcgtgccact ggtggtgctg atcaacggcg
     1261 gcagtgcctc ggcggccgag atcgtcgccg gcgccctgca ggaccacaag cgcggcgtgc
     1321 tgatgggcac cgacagcttc ggcaagggct cggtgcagac cgtgctgccg ctgaccaacg
     1381 agcgcgcgct gaagctgacc accgcgctgt acttcacccc caacggccgc tcgatccagg
     1441 cccagggcat cgtgcccgac atcgtcgtcg agcgcggcaa ggtgacccac gacgacggcc
     1501 cgagccaggg gctcaaggaa gccgacctgt ccggccacct gggcaacggc aacggcggcg
     1561 cggacaagcc gagcaacggc aagcccgcgg cggacaccag caagccgcag gacgaggact
     1621 accagctgag ccaggcgctc aacctgctca agggcctgaa catcacccgc cagccgtaag
     1681 ccggcggggg gcatcactgg gcagggtcgg taactcgcgc agcgattacc gaccattcag
     1741 gccacggccc aaagggcggt agaaggtagg taagcgctcc atgaacctca tctgccaata
     1801 acctgatcat ccgccgatgc tgtactcccg atcattttca ggagccagtc ggcatgatgc
     1861 ggcccaacgc caaggtctcg aaggtctacc tctaccccaa gccggtggat ttccgaaaat
     1921 ccatcgacgg cctggcggcc ctggtcgagc tggatatcaa ggtggcggtg ttcgacccag
     1981 tgctgttcgt gttcctcaac cgggcgcgca accgggtgaa gatactgtac tgggagcgca
     2041 acggcttctg tctgtggctc aagcgcttgg aagccgagcg cttcaaggca ccgccagagc
     2101 ccggtgacga ggccatcgtg ctgagcgccc gggagctgaa ccggctgctg gacggtttca
     2161 atctgtgggg caatcgtccg cacaagatcc tgaccccgct ctacgtggcc tgagccggta
     2221 taatccacgg catgatttcc gtgcccgata cccttcccga cgatcccgcc gcgctcaagc
     2281 agctcttgct ggcattgagc gagcaggtcg ccgtgaagga tgggcaaatt ctccaactgc
     2341 gcgaagaaaa catactcctt cggcaacgcc tgttcgggcg caagtccgag cagacggctg
     2401 acccggccac gccgcaactg gcgctgttca acgaagcgga cagcctagct gaaccgctgg
     2461 acgaggccgc ggacaacgag gtcgtcgaag aggtcgttgt gccctccgcc aagcgtcgcg
     2521 gcaagcgcaa gccgctgccg gccgagctgc cgcgcgtcga ggtgatccac gagctgcccg
     2581 agcacgaact ggcttgcgag tgcggctgcc gtaagcacgt cagcggcgaa gaggtcagcg
     2641 agcagcttga gatcgtgccg atgcagatcc gcgtgctccg tcacatccgt aaggtctacg
     2701 cctgcaaggg ctgcgaaagc gcaccggtca ccgccgacaa gccggcccaa ctgatcgaga
     2761 agagcctggc cagcccgagc gtgctggcga tgctgctgac caccaagtat gtcgacggcc
     2821 tgccgctata ccgcttcgaa aaggtgctgg cccgtcatgg catcgacctg ccgcgacaga
     2881 ccctggcgcg ctgggtgatc caatgcggcg agctgctgca accgctgctc aacctgctgc
     2941 gcgagcacct gctggccagc ccgatgatcc actgcgacga aacccgcgtg caggtgctca
     3001 aggaggccgg gcgagagccg agcagtcaat cctggatgtg ggtgcagacc ggtgggccac
     3061 cggatcgtcc ggtggtgctg ttcgactaca cgaccagccg tgcgcaggag gtgcccttgc
     3121 gcctgctcga gggctacagt ggctacctga tgaccgacga ctacgccggc tacaacgcct
     3181 tggccgcacg accgggcatc gagcggctgg cctgcctggc gcatgcacgg cgcaagttcg
     3241 tcgaggcgca gaaggtgcag cccaagggca agaccgggcg cgccgatgtc gccctgaatc
     3301 tgatcaaccg gctgta
//
LOCUS       DATCUN010000284         3799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_220928, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000284 DATCUN010000000
VERSION     DATCUN010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220928"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /gene="speA"
                     /locus_tag="VIO83_10965"
     CDS             complement(<1..1601)
                     /gene="speA"
                     /locus_tag="VIO83_10965"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012270333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HEY8331832.1"
                     /translation="MVRSTRKDDDSPWSVADSRRVYGIRHWGAGYYAINEAGNVEVRP
                     HGPHGAPIDLHQLVEQLRASGLSLPLLVRFPGILQDRVRQLTGAFDASILRLDYQSDY
                     TALYPIKVNQQEAVVENIIATQDVSIGLEAGSKSELMAVLALAPTGGTIVCNGYKDRE
                     FIRLALMGQKLGHNLFIVIEKESEVALLIEEAAALGVQPQIGLRVRLSSLASSKWADT
                     GGEKSKFGLSAAQILRVAERFRAAGLDQGVRLLHFHMGSQIANIADYRRGFREAIRYY
                     GELRALGLPVDHVDVGGGLGVDYDGTHSRNASSINYDVEDYAATVVGMLKEFCDRQQL
                     PHPHIFSESGRAMTAHHAVLVVEVTDVEQHNDSVPEFDPAAEQPETLQALLELLAPTD
                     PEMVAEIYWRATHLVGEVAAQYSAGKLSLAQKALAEQCYFAICRRLYDQLKARQRSHR
                     QVLDELNDKLADKYICNFSVFQSLPDTWAIEQILPILPIHRLAEQPQRRAVLQDLTCD
                     SDGKIRQYVDEQSIETSLPVHALREG"
     gene            complement(1688..2059)
                     /locus_tag="VIO83_10970"
     CDS             complement(1688..2059)
                     /locus_tag="VIO83_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor Sui1"
                     /protein_id="HEY8331833.1"
                     /translation="MSKKASSLAALGGLVYSTDAGRHCPDCGQPVAACSCGQPVLPEG
                     DGIARVRRETKGRGGKAVTTISGVPLAGDQLKELATALKKRCGTGGALKDGVIEIQGE
                     HVELLLAELAKRGFKAKKSGG"
     gene            complement(2070..3119)
                     /locus_tag="VIO83_10975"
     CDS             complement(2070..3119)
                     /locus_tag="VIO83_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HEY8331834.1"
                     /translation="MLDWKKKVGAMRDNVDDSVADARSYFGGIWASRAVGGVVGVYLL
                     GALVVGWYWSLEPELFPVQENAQGAAAQAQVQLVPGYTTVETLKTVAGTLLHKPGGYL
                     SNDIAPPGLWLDNMPNWEYGVLVQVRDLARALRKDFARSQSQSTEDADLAKAEPKFHF
                     DNSSWALPASEQEYRDGITSLTRFQKRLADPVQNQAQFYARADNLRNWLTDVETRLGS
                     LSQRLSASVGQMRLTTDTSTAITAGSEEIVKTPWLQIDNVFYEARGQAWALSHFLRAI
                     EVDFADVLAKKNATVGVRQIIRELEAAQEPLWSPMILNGDGFGVLANHSLVMANYISR
                     ANAAIIDLRQLLEQG"
     gene            3302..>3799
                     /locus_tag="VIO83_10980"
     CDS             3302..>3799
                     /locus_tag="VIO83_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEY8331835.1"
                     /translation="MPRLLLLLLLLFTLPASAGLFDSRPAPLLGGAPLNNSGDFLPVG
                     EAFKLSLVEADDQRVKLRFVNAEGYYLYRHRFQVQSEPAGLVRGELVLPAGQAKHDEY
                     FGDVEVYYGVTDMEVPLANPSGQAFTLRVTYQGCADQGLCYPPETATLAIDGKAPAGA
                     SPTAPP"
BASE COUNT          539 a         1309 c         1321 g          630 t
ORIGIN      
        1 ccctcgcgca gcgcgtgcac cggcaggctg gtctcgatgc tctgctcgtc gacgtactgg
       61 cggatcttgc cgtcggagtc gcaggtcagg tcctgcagca ccgcgcggcg ctgcggctgc
      121 tcggccaggc ggtggatcgg caggatcggc aggatctgct cgatcgccca ggtgtccggc
      181 aggctctgga acaccgagaa gttgcagatg tacttgtccg ccagcttgtc gttgagctcg
      241 tcgagcacct ggcggtgcga gcgctggcgc gccttgagct ggtcgtacag gcgccggcag
      301 atggcgaagt agcactgctc ggccagcgcc ttctgcgcca gcgacagctt gccggcggag
      361 tactgcgcgg ccacctcgcc gaccaggtgg gtagcccgcc agtagatctc ggccaccatc
      421 tccgggtcgg tcggggcgag cagctcgagc agcgcctgca gcgtttccgg ctgctccgcg
      481 gccgggtcga actccggcac gctgtcgttg tgctgctcga cgtcggtcac ctccaccacc
      541 agtaccgcgt ggtgggcggt catcgcccgg ccgctctcgg agaagatgtg cgggtgcggc
      601 agctgctggc ggtcgcagaa ctccttgagc atgccgacca cggtggcggc gtagtcctcg
      661 acgtcatagt tgatcgagct ggcgttgcgc gaatgggtgc cgtcgtagtc gacgcccagg
      721 ccgccgccga cgtcgacgtg atccaccggc aggccgaggg cgcgcagctc accgtagtag
      781 cggatcgcct cgcggaagcc gcgccggtag tcggcgatgt tggcgatctg cgagcccatg
      841 tggaagtgca gcaggcgcac gccctggtcc agcccggcgg cgcggaaccg ttcggccacc
      901 cgcaggatct gcgcggcgga caggccgaac ttggacttct cgccaccggt gtcggcccac
      961 ttgctcgagg ccagcgagga cagccgcacg cgcaggccga tctgcggctg cacgccgagg
     1021 gcggcggcct cctcgatcag cagcgccacc tcggattcct tctcgatgac gatgaacagg
     1081 ttgtggccga gcttctgccc catcagcgcc aggcggatga actcgcggtc cttgtagccg
     1141 ttgcagacga tggtgccgcc ggtgggcgcc agcgccagca ccgccatcag ctccgacttg
     1201 gagccggcct ccaggccgat ggagacatcc tgggtggcga tgatgttctc caccaccgcc
     1261 tcctgctggt tgaccttgat cggatacagc gcggtgtagt cgctctggta gtccaggcgc
     1321 aggatgctgg cgtcgaaggc gccggtgagc tggcgcacgc gatcctggag gatgccggga
     1381 aagcgcacca gcagcggcag cgacagcccg gaggcgcgca gctgctcgac cagctggtgc
     1441 aggtcgatgg gcgcgccgtg cggaccgtgc gggcgcacct cgacgttgcc ggcctcgttg
     1501 atcgcgtagt agccggcgcc ccagtggcgg atgccgtaga cgcgccggct gtcggccacg
     1561 ctccaggggc tgtcgtcgtc cttgcgggtg ctgcggacca tgcgctgttc tccctgtgct
     1621 ggcgaccggc actccggccg cgctcggatg gtgcgcccgg cccggcaggc cggacgccgg
     1681 gcggcactca gccgccggat ttcttcgcct tgaagccgcg cttggccagc tcggccagca
     1741 acagctcgac gtgctcgccc tggatctcga tcaccccgtc cttgagcgcg ccgccggtgc
     1801 cgcagcgctt cttcagcgcc gtggccagct ccttgagctg gtcgccggcc agcggcacgc
     1861 cgctgatggt ggtcaccgcc ttgccgccgc gccccttggt ctcgcggcgc acgcgggcaa
     1921 tgccgtcgcc ctcggggagc accggctggc cgcagctgca ggcggccacc ggctggccac
     1981 agtccgggca gtgccgcccg gcgtcggtgg agtagaccag gccgccgagg gcggccaggg
     2041 aggaagcctt cttgctcacg tccgcctcct caaccctgct cgagcagctg gcgcaggtcg
     2101 atgatcgcgg cgttggcgcg cgagatgtag ttggccatca ccagcgagtg gttggccagc
     2161 acgccgaagc cgtcgccatt gaggatcatc gggctccaca gcggctcctg cgccgcctcc
     2221 agctcgcgga tgatctgccg cacgccgacc gtggcgttct tcttggccag cacgtcggcg
     2281 aagtcgacct cgatggcgcg caggaagtgc gacagcgccc aggcctggcc gcgggcctcg
     2341 tagaacacgt tgtcgatctg cagccaggga gtcttgacga tctcctcgct gccggcggtg
     2401 atggcggtgc tggtgtcggt ggtcaggcgc atctggccga cgctggcgga caggcgctgc
     2461 gacagcgagc cgaggcgggt ctcgacgtcg gtcagccagt tgcgcaggtt gtcggcgcgg
     2521 gcatagaact gcgcctggtt ctgcaccggg tcggccaggc gtttctggaa gcgggtcagc
     2581 gacgtgatgc cgtcgcggta ttcctgctca ctggccggca gcgcccagct gctgttgtcg
     2641 aagtggaact tcggttcggc cttggccagg tcggcgtcct cggtggactg cgactgcgag
     2701 cgggcgaaat ccttgcgcag ggcgcgggcc aggtcgcgca cctgcaccag cacgccgtat
     2761 tcccagttgg gcatgttgtc cagccacagg ccgggcgggg cgatgtcgtt ggacaggtag
     2821 ccgcccggct tgtgcagcag ggtgccggcc accgtcttca gcgtctccac cgtggtgtag
     2881 ccgggcacca gctgcacctg ggcctgcgcg gcggcgccct gggcgttctc ctgcaccggg
     2941 aacagctcgg gctcgaggct ccagtaccag ccgaccacca gtgccccgag caggtagacg
     3001 ccaaccacgc cgccgaccgc gcggctggcc cagatgccgc cgaagtagct gcgggcatcc
     3061 gccaccgagt catcgacatt gtcgcgcatg gcgccgacct tcttcttcca gtccagcatg
     3121 gcttggtcct ttctcaccga gatacaaatt caggctggca gccccgctcg caaggctgca
     3181 gattgcgttt cactataaag catggccgta gcgggcgtcg gcagaaaagc gcgactgcct
     3241 gccacaacgc gatgccggtc ccttccggta tcatgccggc gttcccgacc gagagactgc
     3301 gatgcctcgc ctgttgctcc tgcttctgct gctgttcacc ctgccggcca gtgccgggct
     3361 gttcgacagt cgtccggccc cgctgctggg tggcgcgccg ctcaacaaca gcggcgactt
     3421 cctgccggtc ggcgaggcct tcaagctcag tctggtcgag gccgatgacc agcgggtgaa
     3481 gctgcgcttc gtcaacgccg aaggctacta cctgtaccgc caccgcttcc aggtacagag
     3541 cgaaccggcc ggactggtcc gcggcgagct ggtgctgccg gcaggccagg ccaagcacga
     3601 cgagtacttc ggcgacgtcg aggtgtacta cggcgtgacc gacatggagg taccgctcgc
     3661 caacccgtcc gggcaggcgt tcacgctgcg cgtcacctac cagggctgcg ccgaccaggg
     3721 cctgtgctat ccgccggaaa ccgccaccct ggccatcgac ggcaaggcgc cggcaggcgc
     3781 gagcccgacc gccccgcca
//
LOCUS       DATCUN010000285        17425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_32883, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000285 DATCUN010000000
VERSION     DATCUN010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 17425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17425
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32883"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VIO83_10985"
     CDS             <1..732
                     /locus_tag="VIO83_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8331836.1"
                     /translation="SRAMLADALGQFLAARRWQRALLIPGPTEGDQAYAAALRRAAKR
                     FGVKIVAEKPWTFDNDQRRSAQAEMPLFTQTAEYDVVLVADERGDFGEYVPYHTWYPR
                     PVAGTQGLTPTGWHKTVETYGAAQLQKRFEEHAGRWMNDRDFAAWIAVRSIATAANKL
                     RSSDAMAIRQLALSAELPLDGFKGRKLSFRDWNGQLRQPIPLVQPRALVTTSPQDGFL
                     HPLTDLDSLGFDRPESRCNLAANGG"
     gene            825..1796
                     /locus_tag="VIO83_10990"
     CDS             825..1796
                     /locus_tag="VIO83_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YVTN family beta-propeller repeat protein"
                     /protein_id="HEY8331837.1"
                     /translation="MHKTLLATLIAAPLLLGAGTTLAATAYVSNEKDNTLSVIDLDKL
                     ETVATLEVGMRPRGLALSHDNQLLYICASDSDRVQVMDLASRKIVKELPSGADPEQFA
                     LHPNNQWLYVSNEDDALVTVVDVNSAQVKAQIEVGVEPEGMAVSPDGKWAINTSETTS
                     MLHWIDTSTHQLVDNTQVDQRPRHVEFSQDGKWLWASAEIGGTVTVVDVATRQPVKTL
                     KFAIKGVHPDKVQPVGVKLTSDGKYAFVALGPANHVAVVDAQTYEVLDYLLVGRRVWH
                     MAFTPGEQQLLTTNGVSGDVSVIDVASRKVTKSIKVGRYPWGVVVTP"
     gene            1793..2515
                     /locus_tag="VIO83_10995"
     CDS             1793..2515
                     /locus_tag="VIO83_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8331838.1"
                     /translation="MIGLQASGVGFAYGARQALVDLDFALVPGRFNALLGPNGAGKST
                     LIALLTRLYDLQGGDIRIGACSIREQPRRALAQIGVVFQQSTLDLDLTVEQNLRYHAA
                     LHGLPRAQAFERIELELARQQLAERRREPVRALNGGHRRRVEIARALLHRPQLLLLDE
                     ASAGLDPASRQALNQHVRRLCVEDGLTVLWTTHLFDEVQADDPLLILHRGRLVARGTA
                     ASLAETGEDLSASFARLTEVAA"
     gene            2646..3437
                     /locus_tag="VIO83_11000"
     CDS             2646..3437
                     /locus_tag="VIO83_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8331839.1"
                     /translation="MTAYLECLRGIVLREWLRFVQQRSRFLSALVRPLLWLLVFAAGF
                     RAALGIAIIEPYDTYITYETYIVPGLACMILLFNGMQGSLSMVYDREMGSMRVLLMSP
                     LPRPFLLAAKLLATSLLSLLQVYAFLAIAWLYGVQPPAWGLLCALPALLLAALMLAAL
                     GLLLSNGIRQLENFAGVMNFVIFPLFFLSSALYPLWKMREASEWLYWLCALNPFSHAV
                     ELVRFALYERLSLTALLVCLGVTALCSALAVLTFNPQHAALKRSA"
     gene            3624..4205
                     /locus_tag="VIO83_11005"
     CDS             3624..4205
                     /locus_tag="VIO83_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated CXXCW motif
                     protein"
                     /protein_id="HEY8331840.1"
                     /translation="MSRLLAAVLCLPLLLGLLPAQAAEEQLFSADGYRLTRYRSPTPA
                     SVDGAQTVDTATLQQLLAGQQPPVLIDVFRRPWLHGQFVSDTAHQNIPGSLWLANVGE
                     GQPEAQWLDYFRHYLAQASGGDRTRALVFYCKSDCWLSWNASKRAAALGYTRLYWYRD
                     GIDAWQQAGRPVQTAKPAPLLREQSHRQLQESR"
     gene            4211..4876
                     /locus_tag="VIO83_11010"
     CDS             4211..4876
                     /locus_tag="VIO83_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8331841.1"
                     /translation="MYKILIADDHPLFREAIHNVISDGFPDSEVMETSDLDSALALTQ
                     EHDDLDLILLDLNMPGMHGLNGLMTLRNEAPTIPVVIVSAEEDKQIVLQAITYGAVGF
                     ITKSSPRAQMTQAIEQILNGNVYLPSDIIRASKPAPRRASHEASGIPPELLQALTRKQ
                     LLVLERMTKGESNKQIAYNLDIAETTVKAHVSAILRKLGVHNRVQAILSAGDIDFSAY
                     LRR"
     gene            5127..5828
                     /locus_tag="VIO83_11015"
     CDS             5127..5828
                     /locus_tag="VIO83_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331842.1"
                     /translation="MSDEDGHDDSLMDRYHHHLLDLYRNPFLTTSAPRRNSSVSTLAI
                     LGIGLLIWGAFKYWQYVLLALLGIAALYAIAALIAGLSRPARPRKVRDHKRIAFETAK
                     TPTQIRITINSSPEFERYFWSADDDKLVEQWRRYHAESVKPIRRNKAAGQPRHATGRC
                     TPQAKATRLSPEAQRIVAQINASPGLAQHAWHAHEAGLLEQWQSYQTYGQLPIRRSKL
                     EKKLARAAQATAGAR"
     gene            5843..6241
                     /locus_tag="VIO83_11020"
     CDS             5843..6241
                     /locus_tag="VIO83_11020"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8331843.1"
                     /translation="MRVIVNIDVPELAPAIDFYRAALGLTLSRTIEEDVAELTGGSSV
                     IYLLQNPAGSHPVSALPFERHYARHWTPVHLDFVVADLAAVARQALNAGARQESECVD
                     WRGSKCITFSDPFGHGFCLIEFAGETYSAG"
     gene            6248..6571
                     /locus_tag="VIO83_11025"
     CDS             6248..6571
                     /locus_tag="VIO83_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HEY8331844.1"
                     /translation="MARPGKTRRPPDDQQRWSQQVTETSNALDLEPGVFTRDDPRAIA
                     ESLKASAECSERRKASPFASAMAMLNFYINRAGRQLSAEQRAVLEAAKDELRELYGRP
                     RRGSR"
     gene            6929..7468
                     /locus_tag="VIO83_11030"
     CDS             6929..7468
                     /locus_tag="VIO83_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331845.1"
                     /translation="MYENETISIEEALDLRASAGKKLDFSCIECGEALRAHRAGENIA
                     AHFEHFQRNNSCSFSAGTTPAYTGIGSMPTYGIEDIRAIEGYEIDKKLTTHGRNAKLV
                     SACKERDDYRCKACRFKLELNEKYVIECHHTKPVALSGEREVSLDELICLYPTCHRIA
                     HTRSEPLTISEIRQARGLT"
     gene            7556..8074
                     /locus_tag="VIO83_11035"
     CDS             7556..8074
                     /locus_tag="VIO83_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331846.1"
                     /translation="MSIETPEIDDCCEKEIYAFFGLAAYWAQILEHSALNLAIVLNLP
                     SAALTSQGIFDNLYDSLTRKTFGQLLKSNKGIVEIPEAEETYLGEALELRNILIHHYF
                     REHAENLVSEVGRREIKTELQLIISKFKRADQILETIYAPLWEKYGVTEEFISEQLEV
                     LRIKAEARDKDT"
     gene            8161..8550
                     /locus_tag="VIO83_11040"
     CDS             8161..8550
                     /locus_tag="VIO83_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331847.1"
                     /translation="MLNRIILAIELIVLCGPAVALLSLGVLYLPAFLIGSVSGGGDWR
                     LGSFMILCGTWGAISLANLAWHTLSKKSNWPGRPIQWFGIATGVAACILCLANLADSK
                     IMIFIFSGPIIATTHLLYLSKIRNLSS"
     gene            8690..8908
                     /locus_tag="VIO83_11045"
     CDS             8690..8908
                     /locus_tag="VIO83_11045"
                     /inference="COORDINATES: protein motif:HMM:NF025733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HEY8331848.1"
                     /translation="MQALPNHICPLCGGANQCAPAQAGSLDVTCWCTTAPVSKAALAR
                     VPAHLVNKACLCPRCAAGLPPPDTTSEG"
     gene            complement(8945..11584)
                     /gene="nahK"
                     /locus_tag="VIO83_11050"
     CDS             complement(8945..11584)
                     /gene="nahK"
                     /locus_tag="VIO83_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator NahK/ErcS'"
                     /protein_id="HEY8331849.1"
                     /translation="MACTSTRPSPASPSADPDGACAAELRVRLAELEARNLKLERINA
                     ALIERVESAGPQGADAYAAFQHSVVLAEQVRERTDALNRAMTELQTSNALLSEARGRA
                     ETAHQHLIDAIESISDAFVLFDAGQRILLFNRRFQALWGDLQNRIRRGMRLTEVKRLA
                     ISSGLVVEEHGGAEQERTLYRLHDGRWVQVSERPTREGGLVILYTDITEVKIHETARR
                     EQAVAQKSRLLQRTMDNLSQGVAVINAEGELELWNGRFLELSGLAPIEAHRPFAEVMA
                     DSELELLTPDSCDAGGRPLREREQRLSDGRVLEVRTHPMPAGGYVNTYTDITERYHYA
                     ETLRESERWVRLITDQVPALIAYISGDLTYEFTNRVYDEWYSWPRGAMLGQAIHAVHS
                     EEHCRLLEPYIERAFAGESVTFEVAERNQAGQERYMLRSYVPNRQPGGEVLGIFVLIR
                     DITERRRTAEALHQAYQNLEKRVRERTAELTAVNSQLREEIDERARAEARLREAKREA
                     EQANLSKTKFLAAVSHDLLQPLNAARLFTSALLEQPVPAPSAGLIRNVSNSLEDVESL
                     LGTLVDVSKLDAGVIKPDVGAFAIADLLDNLAAEYHQIAASEGLTLDFVPSSALVKSD
                     TQLLARVLRNFLSNAIRYTASGRVLLGCRRRPGGLSIEVWDTGAGIAPDKLGEIFQEF
                     KRGAPVRATQDRGLGLGLAIVEKIVRMLGHRIRVYSREGIGSCFAVEVPYARSVVRPR
                     EQSELPPPPGEHLQGARVWVVDNDAAICAGMRTLLEGWGCDVVTALSGDDLARQVDTV
                     QGVADILVVDYHLDNGHTGADLVQELNARRSAPLPVLMITANYSNDLKLQMRELGQVL
                     MHKPVRPMKLKTAMSHLLERGRG"
     gene            complement(11514..12677)
                     /gene="nosP"
                     /locus_tag="VIO83_11055"
     CDS             complement(11514..12677)
                     /gene="nosP"
                     /locus_tag="VIO83_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035991 - nitric oxide sensor activity
                     [Evidence IEA];
                     GO_process: GO:0046209 - nitric oxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing protein NosP"
                     /protein_id="HEY8331850.1"
                     /translation="MEEDLNDVVRMALSESSDPESVAQDLARQLMHPHLGCVLFFCSA
                     EYDLPALANALEQYFGGVRLVGCTTAGEITPQGYGRGCVSAIGFDHRSFSIAAARIDA
                     LDSFSLLDAQQVVAQLAEECRGSSREPISGHSFALTLLDGLSSREELVLSALNAAFGR
                     IPHFGGSAGDDNYLTRTHVYHDGRFHTGAAVVVLVHTALDFEVFTTHHIQPRDEKLVV
                     TAADPASRTVFELNAEPAALEYARLLGLDPQQLDLKTFALHPLAVRLGEQHYVRSIQR
                     VNEDLSLTFYCAVENGIVLTAMQPGPLLPNLQALFDGLEQRLGPLLLTIGCDCFLRRM
                     EVEARGLVADTAGLLVRHRVIGFNTYGEQFNGMHINQTFTGVAIGRPGRGLCR"
     gene            complement(12970..14145)
                     /locus_tag="VIO83_11060"
     CDS             complement(12970..14145)
                     /locus_tag="VIO83_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY8331851.1"
                     /translation="MNNKNKACYSLLAAATALAFSLPAAAEIVLYDKNDTTFSTDGYF
                     NTFYVNSDVDRAGEQFDRTQSRVKMGFLPNYIGFNFGKQVDDLKLGGRASFWVTINDS
                     ETNGTATDIDVRQFYATAANAEWGEVLFGKDFGLFARSNILLDEMLTGYGQVSDTLGL
                     VDGGGVSFGNIGTGYPYPFPTSQITYRSPLMSGLRVAAGIMDPVDTNEDTDSALDKAY
                     QEAPRFETEITYQFEVGGAQIFSWVNGMQQSSENTDDSIEDVDSSGVGYGVQAKMGNL
                     SLTASGFQAEGINPFFTNNAGEALLREVDSDGYLLQGSYRFGKNRVALSYGKTEDDGN
                     GLGSAADYQTRGIALFHDVNDNLKLVAELNQFEIEGQDSDALDEDTRTFAVGAALSF"
     gene            14518..15453
                     /locus_tag="VIO83_11065"
     CDS             14518..15453
                     /locus_tag="VIO83_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008369598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY8331852.1"
                     /translation="MLDILAITTPIFLIIGLGFLSARSSLLSREQIRGIGSFVIHFAL
                     PALLIHALTTRPIEEVIKPGYLLAYGLASLAVFAVGVALSRWLRGDNLQGSAMAGLGM
                     SASNTGFIGYPVAAMVIGPTAAAIPMALGMLVETLLMIPLALALAEAGRQSGGGARQV
                     LVQTARRLSRNPMLISMLVGLSLSLLELRLPSILLHSVELLAQASAPAALFAIGGSLY
                     GLKPGGLLAEVSQVALGKLLLHPLAVYVAFSLVPSVDPTLKVAALLFASAPMMSIFPL
                     LGQQYGLENRCSAALVTATALSFLTMSALLGILGI"
     gene            15554..16525
                     /gene="arsS"
                     /locus_tag="VIO83_11070"
     CDS             15554..16525
                     /gene="arsS"
                     /locus_tag="VIO83_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339360.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HEY8331853.1"
                     /translation="MHATLPLLNALDFPALHRGALEALQVNLTYRCNQRCLHCHVNAG
                     PTRHESMDDANVAFLRQVLDSHPLHTLDLTGGAPELHPRFRQIVRHARRAGRRVIDRC
                     NLTILSEPGQADLAEFLAAEGVEVCASLPCYGRDNVDQQRGDGVFARSIAGLRQLNAL
                     GYGAGHGLVLNLVYNPQGPSLPPPQAQLEADYKAHLAAEFGIVFDHLLTLANQPIARF
                     GSTLVSRGQFGAYLQLLRDSYRAENLDALMCRRLLSVDWQGYLYDCDFNQMLGLPLQL
                     PGLLGRDRAHLRDLLNAELDGNPIVTRDHCYACTAGQGSSCGGALKA"
     gene            16528..>17425
                     /locus_tag="VIO83_11075"
     CDS             16528..>17425
                     /locus_tag="VIO83_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY8331854.1"
                     /translation="MDRRLALLGLLLALAALFFVLDLGQYLDLSRLKAEQAALAAGVA
                     AHPWRAAGLFALVYVAVTALSLPGAALLTLVGGALFGLLAGTVLVSLASTLGASLAML
                     GSRFVLRDWVQARFGPRLAAIDRGLERDGAFYLFALRLVPVFPFFLINLAMGPTRLPL
                     RTFAWVSQLGMLPGTLVYVNAGRELGRLDSLADILSPGLLGAFALLGLFPLLARRVLD
                     GLKARRVYAGWPRPRHFERNLVVIGAGSGGLVSAYLAAALQARVTLIEKGQMGGDCLN
                     RGCVPSKALLRSARVASELRKAA"
BASE COUNT         2911 a         5985 c         5578 g         2951 t
ORIGIN      
        1 agccgcgcca tgctcgccga cgccctcggc cagttcctcg ccgcgcgccg ctggcagcgc
       61 gccctgctga ttcccggccc gaccgaaggc gaccaggcct acgccgcggc cctgcgccgc
      121 gccgccaagc gcttcggggt gaagatcgtc gccgagaagc cgtggacctt cgacaacgac
      181 cagcggcgca gcgcccaggc cgagatgccg ctgttcaccc agaccgccga gtacgacgtg
      241 gtgctggtgg ccgacgagcg cggcgacttc ggcgagtacg tgccctacca cacctggtac
      301 ccgcgcccgg tggccggcac ccagggcctg acccccaccg gctggcacaa gacggtggaa
      361 acctacggcg ccgcccagct gcagaagcgc ttcgaggagc acgcagggcg ctggatgaac
      421 gaccgcgact tcgccgcctg gatcgccgtg cgcagcatcg ccaccgcggc caacaagctg
      481 cgcagcagcg acgccatggc gatccgccag ctggcgctgt ccgccgagct accgctggac
      541 ggcttcaagg gccgcaagct gagcttccgc gactggaatg gccagctgcg ccagccgatc
      601 ccgctggtgc agccgcgcgc gctggtcacc acctcgccgc aggacggctt cctgcacccg
      661 ctcaccgacc tcgacagcct gggcttcgac cgcccggaaa gccgctgcaa tctggccgcc
      721 aacggcggct gacgcgtggt gcgcacggcg caccctacaa ggtcgcaatc gtagggtgcg
      781 ccgtgcgcac caccgaaccg cccgataaca acaagaggca caccatgcac aagaccctgc
      841 tcgccacgct gatcgccgcc ccgctgctgc tcggcgccgg cacgaccctc gccgccaccg
      901 cctacgtgtc caacgagaag gacaacaccc tcagcgtgat cgatctggac aagctggaga
      961 cggtggcgac cctcgaggtc ggcatgcgcc cgcgcggcct ggcgctgtcc cacgacaacc
     1021 agctgctgta catctgcgcc agcgactccg accgcgtgca ggtgatggac ctggccagcc
     1081 gcaagatcgt caaggaactg ccctccggcg ccgaccccga gcagttcgcc ctgcacccca
     1141 acaaccagtg gctgtacgtc tccaacgagg acgacgcgct ggtcaccgtg gtcgacgtca
     1201 acagcgcgca ggtcaaggcg cagatcgagg tcggcgtcga gccggaaggc atggcggtca
     1261 gccccgacgg caagtgggcg atcaacacca gcgaaaccac cagcatgctg cactggatcg
     1321 acaccagcac ccaccagctg gtggacaaca cccaggtcga ccagcgcccg cgccacgtcg
     1381 agttcagcca ggacggcaaa tggctgtggg cctcggcgga gatcggcggc acggtgacgg
     1441 tggtcgacgt ggccacccgc cagccggtca agacgctcaa gttcgcgatc aagggcgtgc
     1501 acccggacaa ggtgcagccg gtgggggtca agctgaccag cgacggcaag tacgccttcg
     1561 tcgccctcgg cccggccaac cacgtggcgg tggtcgacgc ccagacctac gaggtgctcg
     1621 actacctgct ggtcggccgc cgcgtctggc acatggcctt cacccccggc gagcagcagc
     1681 tgctgaccac caacggggtc agcggcgacg tctcggtgat cgacgtggcc agccgcaagg
     1741 tcaccaagtc gatcaaggtc ggccgctacc cgtggggcgt ggtggtgacg ccatgatcgg
     1801 cctgcaggcc tccggggtcg gcttcgccta tggcgcgcgc caggcgctgg tcgacctcga
     1861 cttcgccctg gtgcccggac gcttcaacgc cctgctcggc cccaacggcg ccggcaagtc
     1921 gaccctgatc gccctgctca cccgcctgta cgacctgcag ggcggcgaca tccgcatcgg
     1981 cgcctgctcg atccgcgagc agccgcgccg cgcgctggcg cagatcggcg tggtgttcca
     2041 gcagagcacc ctcgatctgg acctgaccgt cgaacagaac ctgcgctatc acgccgccct
     2101 gcacggtctg ccgcgcgcgc aggccttcga gcgcatcgag ctggaactgg cccgccagca
     2161 gctcgccgaa cgccgccgcg agccggtgcg cgcgctcaac ggcggccacc gccgccgggt
     2221 ggagatcgcc cgcgccctgc tgcaccggcc gcaactgctg ctgctcgacg aggccagcgc
     2281 cggtctcgac ccggccagcc gccaggcgct caaccagcat gtgcgccgcc tgtgcgtcga
     2341 agacggcctg accgtgctgt ggaccaccca cctgttcgac gaggtgcagg ccgacgaccc
     2401 gctgctgatc ctgcatcgcg gccgcctggt cgcccgcggc acggcagcga gcctggccga
     2461 aaccggcgaa gacttgtcgg cctccttcgc ccgcctgacg gaggtcgccg catgagcttc
     2521 cctgccctct gggtggtgga aaatcgctgc gcgagttttc caccctacgc attgccaccc
     2581 gtagggtgga taacggccgc aggccttatc caccctgatt gccgcaccgt cgcgagagca
     2641 ttgccatgac cgcctacctg gaatgcctgc gcggcatcgt gctgcgcgaa tggctgcgct
     2701 tcgtccagca gcgctcgcgc ttcctcagcg cgctggtgcg cccgctgctg tggctgctgg
     2761 tgttcgccgc cggtttccgc gccgcgctgg gcatcgccat catcgagccc tacgacacct
     2821 acatcaccta cgagacctac atcgtccccg gcctggcctg catgatcctg ctgttcaacg
     2881 gcatgcaggg ctcgctgtcg atggtctacg accgcgagat gggcagcatg cgcgtgctgc
     2941 tgatgagtcc gctgccgcgg cccttcctgc tcgccgccaa gctcttggcc accagcctgc
     3001 tgtcgctgct gcaggtctat gccttcctcg ccatcgcctg gctgtacggc gtgcagccgc
     3061 cggcctgggg cctgctctgc gccctgccgg cgctgctgct cgccgcgctg atgctcgccg
     3121 cgctgggcct gctgctgtcc aacggcatcc gccagctgga gaacttcgcc ggggtgatga
     3181 acttcgtgat cttcccgctg ttcttcctgt cctcggcgct ctacccgctg tggaagatgc
     3241 gcgaggccag cgagtggctg tactggctgt gtgcgctcaa cccgttcagc cacgcggtgg
     3301 aactggtgcg cttcgccctt tacgagcgcc tgagtctcac cgccctgctg gtctgcctgg
     3361 gcgtcacggc gctgtgcagc gcgctggccg tgctgacctt caacccgcag cacgcggcgc
     3421 tcaagcgcag tgcctgagtg tccgtagggt gcgccctgcg caccaatgct ccgcggctcg
     3481 gccaatggtg cgccgtgcgc accctgcatg acgacttttc gcgaaattac tactttggta
     3541 ctgcctttcg cctccatcgt aggattccca aggcactgcg actcactgat aaatagtcgc
     3601 aaataacaag ggaaacccca gccatgtcgc gcctgcttgc cgccgtgctc tgtctgccgc
     3661 tgctcctcgg cctgctcccc gcccaggccg ccgaggaaca actgttctcc gccgacggct
     3721 atcgcctgac ccgctaccgc agcccgaccc cggccagcgt ggacggcgcg cagaccgtcg
     3781 acaccgccac cctgcagcag ctgctcgccg gccagcagcc gccggtgctg atcgacgtgt
     3841 tccgccgccc ctggctgcac ggccagttcg tcagcgacac cgcccaccag aacatccccg
     3901 gcagcctgtg gctggccaac gtcggcgaag gccagcccga ggcgcagtgg ctggactact
     3961 tccgccatta cctcgcgcag gccagcggtg gcgaccgcac ccgcgccctg gtgttctact
     4021 gcaagtccga ctgctggctg tcgtggaatg ccagcaaacg cgccgccgcg ctgggctata
     4081 cccggctgta ttggtaccgt gacggcatcg acgcctggca acaggccggc cggccggtgc
     4141 aaaccgccaa gccagccccg ctgctccgcg agcagtccca tcgacaacta caagaatcca
     4201 ggtgaaggcc atgtacaaga tcctcatcgc cgatgaccac ccgctgttcc gcgaggccat
     4261 ccacaacgtc atcagcgacg gcttcccgga cagcgaggtg atggaaacct ccgacctcga
     4321 cagcgccctg gccctgaccc aggaacacga cgaccttgac ctgatcctgc tcgacctcaa
     4381 catgcccggc atgcacggcc tcaacggcct gatgaccctg cgcaacgagg cgccgaccat
     4441 cccggtggtg atcgtctccg ccgaggagga caagcagatc gtcctgcagg cgatcaccta
     4501 cggcgcggtc ggcttcatca ccaagtcctc gccgcgcgcg cagatgaccc aggccatcga
     4561 gcagatcctc aacggcaacg tctacctgcc ctccgacatc atccgcgcca gcaagccggc
     4621 cccgcgccgc gccagccacg aggcctcggg catcccgccg gaactgctgc aggcgctgac
     4681 ccgcaagcag ctgctggtgc tggagcggat gaccaagggc gagtccaaca agcagatcgc
     4741 ctacaacctc gacatcgccg aaaccacggt caaggcccac gtctccgcca tcctgcgcaa
     4801 gctcggcgtg cacaaccgcg tgcaggccat cctcagcgcc ggcgacatcg acttcagcgc
     4861 ctacctgcgc cgctgagccc gcacctttct cctgcccaca gggaagtggg ccccgggttg
     4921 cccatatcgc cccacagcca acctgcccgc ccgcacaagc ccctccgccc gactcctgca
     4981 ccccatcgct catgaactag gctgaaccct gtagctccgg cgtctcgccg tggcaacaga
     5041 gccgcttccc ctgggccatg cgcaatgcac cggcgggctg cgaatccgtg tgaagccagg
     5101 ttcgaggagg ccggcgggaa aaccccatga gcgacgaaga cgggcatgac gactccttga
     5161 tggacaggta ccaccatcac ctgctggacc tttaccggaa tccgttcctc accacctcgg
     5221 cccccaggcg gaactcgagc gtctccaccc tggcaatcct ggggataggg ctgctcatct
     5281 ggggagcatt caagtattgg cagtacgtcc tgctggcttt gctggggata gccgcgttgt
     5341 acgccatcgc cgccctgatc gccgggctga gccggccagc caggccccga aaagtccgcg
     5401 accataaacg aatagctttc gagaccgcca agaccccgac gcaaattagg atcaccatca
     5461 acagctcgcc cgaattcgag cgctacttct ggtccgccga cgatgacaag ctggttgagc
     5521 aatggcggcg ctaccacgca gagagcgtca agccgatccg gcgcaacaag gcagccgggc
     5581 agccgcgcca cgccacaggg cggtgtaccc cgcaggcgaa agccacgcgc ctgagccccg
     5641 aggcgcagag gatcgtcgcg cagatcaatg cctcgccggg actggcgcaa cacgcctggc
     5701 acgcgcacga ggccggcctg ctcgagcagt ggcagagcta ccagacctac ggccagctgc
     5761 cgatcaggcg cagcaagctg gaaaagaaac tggccagagc cgcccaggcc actgcagggg
     5821 caaggtgacc ggaggctgag ccatgcgcgt gatcgtgaat atcgacgtcc ccgagctcgc
     5881 gccagcgata gatttctacc gcgcagcact cggattgacg ctgagccgca ccattgaaga
     5941 ggatgtcgca gagctgactg gtggctcttc cgtgatctac cttctacaaa atcccgccgg
     6001 ctcacacccc gtgagtgcac tcccgtttga gcgccattac gcgagacatt ggacccccgt
     6061 gcatctggac ttcgtcgtgg ccgacctcgc cgcagtcgca cgccaggcat tgaacgccgg
     6121 ggcacgccag gagagcgaat gcgtcgactg gcggggctcg aagtgcatca ccttctcgga
     6181 tccattcggc cacggcttct gcctgatcga gtttgccggg gaaacctaca gcgccggctg
     6241 agcactcatg gccaggcccg gaaaaacccg ccgtccgcca gacgaccagc aacgctggtc
     6301 gcagcaggtg accgagacca gcaacgccct ggacctcgaa ccgggggtgt tcacccggga
     6361 cgacccgcgc gccatcgccg agtcgctcaa ggcctccgcc gaatgcagcg agcggcgcaa
     6421 ggcctctccc ttcgcctcgg ccatggcgat gctcaacttc tacatcaacc gcgccggccg
     6481 ccagctctcc gccgagcagc gtgcggtgct ggaggcggcc aaggacgagc tgcgcgagct
     6541 gtacggcagg ccgcggcgcg gcagcaggtg agacgcgcgc cacgacacac ctactcggcc
     6601 gcgaagcagt acaacaagcc cgccccaccc gaggcttccc actcgccacg catccatctg
     6661 tcgcgcctcg tggcatggac agccttgcgg gcattcgggt agcgtcaccg catacccgct
     6721 cgcgcaacgg ggcgaacggc aagggcttgc caatcttggt ggataacatc ccgcaggctt
     6781 ccggtaagga gctgaaagcg gtaacgcagg tggagtacgc caggtttgac gagggaatgt
     6841 tatgcaccag atggggcact tgggggcgcc atttggtttc gaatagtagt taggtaatcc
     6901 attatttatg cgcgcggcca tagagtgcat gtacgaaaat gaaaccatca gcattgaaga
     6961 ggctctagat cttcgagcga gcgctggtaa gaaattagat ttttcgtgca ttgagtgcgg
     7021 ggaggcacta agggctcaca gagcaggtga aaatattgcc gctcactttg agcactttca
     7081 aagaaacaac tcatgctctt ttagtgcagg aacgacacct gcatatactg gcatcggctc
     7141 catgccaact tatggcattg aagacataag agccattgaa ggctacgaaa ttgacaaaaa
     7201 gctaactacg cacggcagaa atgccaagct tgtaagtgcg tgcaaagagc gcgatgacta
     7261 tagatgcaaa gcgtgcagat ttaaacttga actaaatgag aaatatgtaa ttgagtgcca
     7321 ccacacaaaa cccgtcgcac ttagcggaga acgagaagtt tctctggatg agttaatttg
     7381 cttgtaccca acttgccacc gcattgcaca tacaaggagc gagccattaa caatttcgga
     7441 aatcaggcag gcgcgagggc taacctaaca atgcaattaa atcgctcatt tcgttcgttg
     7501 ggacgggcta aagcccgctc tttagcttaa tcgttataca cctaggtaat ttgctatgag
     7561 catagagact ccagaaatcg atgactgctg cgaaaaagag atctatgcat tttttggtct
     7621 tgcagcatac tgggcgcaga tcctagaaca ctcagcattg aatcttgcga tcgttctcaa
     7681 tctccctagt gctgccctta cgagccaggg catctttgac aatttgtatg actcactaac
     7741 aaggaaaacc tttggtcaat tactgaaatc gaataaggga atcgtagaaa tacccgaagc
     7801 ggaagagaca tatctaggcg aagccctaga acttagaaac atattaattc accactactt
     7861 cagagagcac gccgaaaatc ttgtttccga ggtaggccgc agagaaataa aaacagagct
     7921 gcagttaatt atttccaaat tcaagcgcgc agaccaaatc ctcgaaacga tttatgctcc
     7981 tttatgggaa aaatatggtg tgactgagga attcataagc gaacagctag aagtacttcg
     8041 catcaaagcc gaagctcgag ataaagatac ataacaatgc gttaaacaca gttcgcttcg
     8101 cttactggac tcgcaaaagc tgcgtttttt cttgccgatc gacctaattc ttaggatgcc
     8161 atgctaaatc gcatcatatt agccatagag ctcatcgtcc tgtgtggccc ggcagtcgcc
     8221 cttctctccc tgggagttct ttacctgcca gccttcctca ttgggtccgt atcgggcggc
     8281 ggcgattggc gcctgggatc gttcatgatc ctctgcggaa catggggggc gatttcactg
     8341 gccaatctgg cgtggcatac gctcagcaaa aagagcaact ggcccgggcg acccattcag
     8401 tggtttggca tcgcgacagg ggtagccgcc tgcatcttat gcctggccaa ccttgccgac
     8461 tccaaaatca tgattttcat tttttccggc cccattatcg ccacaactca tcttctgtat
     8521 ttatcaaaaa tacggaattt gagttcctga caaggcgctc acttcgttcg ccgggacgct
     8581 gccagcgcaa tgccctcggc ctgagcttta tggttatggt gcgcatggcg caccgcgagt
     8641 ctaaagtggc gtcaggccat cccctctcac atcggcaatc gacccaacca tgcaagcgct
     8701 acccaaccac atatgcccgc tttgcggcgg tgccaaccaa tgcgcccccg cccaggcggg
     8761 aagcctcgac gtgacgtgct ggtgcaccac ggccccggtc agcaaggcgg cactggcgcg
     8821 agtgcccgcc catctggtca acaaggcttg cctgtgcccg cgttgcgcgg ccgggctgcc
     8881 gccgccggac actacaagcg aaggctgaag ctcgtcagcc tggctggccg gccaagcgga
     8941 taccctaccc cctcccccgc tccaacaaat ggctcatcgc cgtcttcaac ttcatcggcc
     9001 gcaccggctt gtgcatcagc acctggccga gttcgcgcat ctgcagcttg aggtcgttgc
     9061 tgtagttggc ggtgatcatc agcactggca gcggcgcgct gcgccgggcg ttgagttcct
     9121 gaaccagatc ggcgccggtg tggccgttgt cgaggtggta gtcgacgacc aggatgtcgg
     9181 ccacgccctg cacggtgtcg acctggcggg ccagatcgtc gccggatagc gcggtgacca
     9241 cgtcacagcc ccagccttcg agcagggtgc gcatgccggc gcagatcgcc gcgtcgttgt
     9301 cgaccaccca gacccgtgcg ccctgcaggt gttcgccggg cggcggcggc agttcgctct
     9361 gctcgcgcgg gcgcaccacg ctgcgcgcgt agggcacctc gacggcgaag caggagccga
     9421 tgccctcgcg cgaatacacg cggatgcggt ggccgagcat gcgcacgatc ttctcgacga
     9481 tggccaggcc gagaccgagg ccgcggtctt gggtggcgcg caccggggcg ccgcgcttga
     9541 actcctggaa tatctcgccg agcttgtccg gggcgatgcc ggcgccggtg tcccacacct
     9601 cgatggacag cccgccggga cggcgccggc agccgagcag cacgcgaccg ctggcggtat
     9661 agcggatggc gttgctgagg aagttgcgca gcacccgcgc cagcagctgg gtgtcgctct
     9721 tgaccagcgc cgagctgggc acgaagtcga gggtcagccc ttcgctggcg gcgatctggt
     9781 ggtattcggc ggcgaggttg tcgagcaggt cggcgatggc gaaagcgccg acgtcgggct
     9841 tgatcacccc ggcatccagc ttggacacgt cgaccagggt gccgagcagg ctttccacgt
     9901 cctccagcga gttgctgacg ttgcggatca gcccggcgct gggcgccggc accggctgtt
     9961 cgagcagcgc gctggtgaac agccgtgcgg cgttgagcgg ctgcaacagg tcgtggctga
    10021 ccgcggcgag gaacttggtc ttcgacaggt tggcctgttc ggcctcgcgc ttggcctcgc
    10081 gcaggcgcgc ctcggcgcgg gcgcgctcgt cgatctcctc gcgcagctgg ctgttcaccg
    10141 cggtcagttc ggcggtgcgc tcgcgcacgc gcttctcgag gttctggtag gcctggtgca
    10201 gcgcctcggc ggtgcgccgg cgttcggtga tgtcgcggat cagcacgaag atgccgagca
    10261 cctcgccgcc cggctggcgg ttgggcacgt aggaacgcag catgtagcgc tcctggccgg
    10321 cctggttgcg ttcggccacc tcgaaggtga cgctctcgcc ggcgaaggcg cgctcgatgt
    10381 agggctcgag cagccggcag tgctcctcgc tgtgcaccgc gtggatggcc tggccgagca
    10441 tggcgccgcg cggccagctg taccactcgt cgtagacgcg gttggtgaac tcataggtga
    10501 ggtcgccgct gatgtaggcg atcagcgccg gcacctggtc ggtgatcagg cgcacccagc
    10561 gctcgctctc gcgcagggtt tcggcgtagt ggtagcgctc ggtgatgtcg gtgtaggtgt
    10621 tgacgtagcc gccggccggc atcggatggg tgcgcacttc cagcacgcgg ccgtcgctca
    10681 ggcgctgttc gcgctcgcgc agcgggcggc cgccggcatc gcagctgtcc ggggtgagca
    10741 gctcgagctc gctgtcggcc atcacctcgg cgaacggccg atgcgcctcg atcggcgcca
    10801 ggccgctgag ttcgaggaag cggccgttcc acagctccag ctcgccctcg gcgttgatca
    10861 ccgcgacgcc ctgggacagg ttgtccatgg tccgctgcag caggcgcgac ttctgcgcca
    10921 ccgcctgttc gcggcgcgcg gtctcgtgga tcttcacctc ggtgatgtcg gtgtagagga
    10981 tcaccaggcc gccctcgcgg gtcggccgct cgctgacctg cacccagcgg ccgtcgtgca
    11041 ggcggtagag ggtgcgctcc tgctcggcgc cgccgtgttc ctcgaccacc agcccggagc
    11101 tgatcgccag gcgcttcacc tcggtcaggc gcatgccgcg gcggatgcgg ttctgcaggt
    11161 cgccccacag cgcctggaag cggcggttga acagcaggat gcgctgcccg gcgtcgaaca
    11221 gcacgaaggc gtcggagatg ctctcgatgg cgtcgatcag gtgctgatgg gcggtctcgg
    11281 cgcggccgcg cgcctcgctg agcagcgcat tgctggtctg cagctcggtc atcgcgcggt
    11341 tcagcgcgtc ggtgcgctcg cgcacctgct cggcgagcac caccgagtgc tggaaggcgg
    11401 cgtaggcgtc ggcgccctgc ggaccggcag actcgacgcg ctcgatcagc gcggcgttga
    11461 tccgctccag cttgaggttg cgcgcctcca gctcggcgag ccggacgcgc agctcagcgg
    11521 cacaggcccc gtccgggtcg gccgatggcg acgccggtga aggtctggtt gatgtgcatg
    11581 ccattgaact gctccccgta ggtgttgaaa ccgatcacgc ggtggcggac cagcaggccg
    11641 gcggtgtcgg cgaccaagcc gcgcgcttcc acctccatgc ggcgcaggaa gcagtcgcag
    11701 ccgatggtca gcagcagcgg gccgagacgc tgctccaggc cgtcgaacag cgcctgcagg
    11761 ttgggcagca gcggcccggg ctgcatggcg gtgaggacga tgccgttctc caccgcgcag
    11821 tagaaggtca ggctgaggtc ctcgttgacc cgctggatcg agcgcacgta gtgctgctcg
    11881 ccgaggcgca ccgccagcgg gtgcagggcg aaggtcttga ggtcgagctg ctgcggatcg
    11941 aggccgagca ggcgcgcgta ctccagcgcc gccggctcgg cgttgagctc gaacaccgtg
    12001 cggctggccg ggtcggcggc ggtgaccacc agcttctcgt cgcgcggctg gatgtggtgg
    12061 gtggtgaaca cctcgaaatc cagcgcggtg tgcaccagca ccaccaccgc ggcgccggtg
    12121 tggaagcggc cgtcgtggta gacgtgggtg cgggtcaggt agttgtcgtc gccggccgag
    12181 ccgccgaagt gcgggatgcg cccgaaggcg gcgttcagcg ccgagagcac cagctcctcg
    12241 cggctggaca ggccgtcgag cagggtcagc gcgaagctgt ggccgctgat cggctcgcgg
    12301 ctgctgccgc ggcattcctc ggccaactgg gcgaccacct gctgggcgtc gagcaggctg
    12361 aagctgtcca gcgcgtcgat gcgcgcggcg gcgatggaga agctgcggtg gtcgaagccg
    12421 atggcgctga cgcaaccgcg gccgtagccc tgcggggtga tctcgccggc ggtggtgcag
    12481 ccgaccaggc gcacgccgcc gaagtactgc tccagggcgt tggccagggc gggcaggtcg
    12541 tattcggcgc tgcagaagaa cagcacgcag cccaggtgcg ggtgcatcag ctggcgggcc
    12601 aggtcctggg ccacgctttc cgggtcgctc gactcggaaa gggccatgcg gacgacgtcg
    12661 ttcagatctt cttccacgtc gcctccggac cggcagaaca cacagacggg atgattctag
    12721 gaaggtcgcg gcgggcagtt aatgctactt gggtactgcc cgcctgcgcc tttgggggta
    12781 cagcatgaaa agtccgggac ccggcggcag gaaaagaccc cacggtcgtt ttccagcctg
    12841 cctttcccgc ttcgatcgaa caccgcctgg aggatggaaa acccgctcga cgttttttcc
    12901 accgcgacgc gccaggtgga aaagacccac ggtcgttttc cacccttccc caggccgtgg
    12961 ctggcggcct tagaagctca gcgccgcacc gacggcgaag gtgcgggtgt cctcgtcgag
    13021 ggcatcgctg tcctggcctt cgatctcgaa ctggttgagc tcggcgacca gcttgaggtt
    13081 gtcgttgacg tcgtggaaca gcgcgatgcc gcgggtctgg tagtcggcgg cgctgcccag
    13141 gccgttgccg tcgtcctcgg tcttgccgta ggacagcgcg acgcggttct tgccgaagcg
    13201 gtaggagccc tgcagcaggt agccgtcgct gtcgacttcg cgcagcagcg cctcgccggc
    13261 gttgttggtg aagaacgggt tgatgccctc ggcctggaag ccggaggcgg tcagcgacag
    13321 gttgcccatc ttggcctgca cgccgtagcc gacgccggag gagtcgacat cctcgatgct
    13381 gtcgtcggtg ttttccgagc tctgctgcat gccgttgacc caggagaaga tctgcgcgcc
    13441 gccgacttcg aactggtagg tgatctcggt ttcgaagcgc ggcgcctcct ggtaggcctt
    13501 gtccagggcg gagtcggtgt cttcgttggt gtccaccggg tccatgatgc cggcggcgac
    13561 gcgcaggccg ctcatcagcg gcgagcggta ggtgatctgc gaggtcggga acgggtacgg
    13621 gtagccggtg ccgatgttgc cgaacgacac gccgccgcca tccaccaggc cgagggtgtc
    13681 gctgacctgg ccgtagccgg tgagcatttc gtcgagcagg atgttggagc gggcgaacag
    13741 gccgaagtcc ttgccgaaca gcacctcgcc ccactcggcg ttggcggcgg tggcgtagaa
    13801 ctggcgcacg tcgatgtcgg tggcggtgcc gttggtctcg ctgtcgttga tggtcaccca
    13861 gaacgaggcg cggccgccga gcttgaggtc gtcgacctgc ttgccgaagt tgaagccgat
    13921 gtagttgggc agaaagccca tcttgacccg cgactgggtg cggtcgaatt gctcgccggc
    13981 acggtcgacg tcgctgttga cgtagaaggt gttgaagtag ccgtcggtgg agaaggtggt
    14041 gtcgttcttg tcgtacagca cgatttcggc agccgccggc aggctgaatg ccagggcagt
    14101 ggcagcggcc agcagggaat agcaggcttt gttcttgttg ttcatcttgg tctccgcttg
    14161 cgtcaggacg actgcctgat ccaacagtcg cgccgattat ccggagacgg ccggtagcac
    14221 ccgtattctg cgcaagcgcc ggatgccttg tgctttggca ggccagtacg gcgcacgccg
    14281 aaagccgctc atccccggca ggcggaagtc gtgcgactcc tgcacgcctt atgatgcttt
    14341 ggaagtaagc gcgctggttt tgaaagcttt tcgttcgctg atcgcacgaa aaaccggtga
    14401 aacgttcagg ccgcctttca cccggcgcga cgagctgcct ataatgggcg cggccaggat
    14461 cgacgcctgc ccctttcgcc ccctgaccgc caggcggcca ccccgccgga ccaccccatg
    14521 ctcgacatcc ttgccatcac cacgcccatc ttcctcatca tcggcctcgg cttcctgtcc
    14581 gcgcgcagca gcctgctcag ccgcgagcag atccgtggca tcggcagctt cgtcatccat
    14641 ttcgccctgc ccgccctgct gatccacgcg ttgaccaccc ggccgatcga ggaggtgatc
    14701 aagcccggct acctgctggc ctacggactg gcctcgctgg cggtgttcgc ggtgggggtg
    14761 gcgctgtcgc gctggctgcg cggcgacaac ctgcagggct cggccatggc cgggctgggc
    14821 atgtcggcct ccaacaccgg cttcatcggc tacccggtgg ccgccatggt catcggcccg
    14881 acggccgccg cgatccccat ggcgctgggc atgctggtgg aaaccctgct gatgatcccg
    14941 ctggccctgg cactggccga ggctggccgg cagagcggtg gcggcgctcg tcaggtgctg
    15001 gtgcaaaccg cgcgccgtct gtcccgcaac ccgatgctga tcagcatgct ggtcggcctg
    15061 agcctgtcgc tgctggagct gcgcctgccg tcgatcctgc tgcacagcgt cgagctgctc
    15121 gcccaggctt cggcgccggc ggcactgttc gccatcggcg gctcgctgta cgggctcaag
    15181 cccggcggcc tgctggcgga ggtcagccag gtggccctgg gcaagctgct gctgcacccg
    15241 ctggcggtgt acgtcgcctt cagcctggtg ccctcggtcg accccaccct caaggtcgcc
    15301 gccctgctgt tcgccagcgc cccgatgatg agcatcttcc cgctgctggg tcagcagtat
    15361 ggcctggaga accgctgctc cgcggcgctg gtcacggcca ccgcgctgtc gttcctgacc
    15421 atgagcgcgc tgctggggat attggggatc tagtaggcgc gcgaccgata gccacacgcc
    15481 cgccgccggg cgagtgccgg cgcagggact atccttgcag gacccttcag gcccgcgccg
    15541 ggacccaccc accatgcacg ccaccctgcc gctgctcaac gccctggact ttcccgccct
    15601 gcaccgcggc gcgctggaag ccctgcaggt caacctgacc taccgctgca accagcgctg
    15661 cctgcactgc cacgtcaacg ccgggccgac gcgccacgag agcatggacg acgccaacgt
    15721 ggcgttcctg cgccaggtgc tcgacagcca tccgctgcac accctcgacc tgaccggcgg
    15781 cgcgcccgag ctgcacccgc gcttccggca gatcgtccgc catgcgcggc gcgccgggcg
    15841 gcgggtgatc gaccgctgca acctgaccat cctcagcgag cccgggcagg cggatctggc
    15901 cgagtttctc gccgccgagg gcgtcgaggt ctgcgcctcg ctaccctgct acggccggga
    15961 caacgtcgac cagcagcgcg gcgacggcgt gttcgcccgc agcatcgccg gcctgcgcca
    16021 gctcaatgcc ctgggctacg gcgcgggcca cgggctggtg ctcaacctgg tctacaaccc
    16081 gcaggggccg agcctgccgc cgccgcaggc gcagctggag gccgactaca aggcgcacct
    16141 ggccgcggag ttcggcatcg tcttcgacca cctgctgacc ctcgccaacc agccgatcgc
    16201 ccgcttcggc agcaccctgg tcagccgcgg ccagttcggc gcctacctgc aattgctgcg
    16261 cgacagctac cgcgcggaaa acctcgacgc gctgatgtgc cggcgcctgc tcagcgtcga
    16321 ctggcagggc tacctgtacg actgcgactt caaccagatg ctcggcctgc cgctgcagct
    16381 gccgggcctg ctcggccgcg accgcgccca cctgcgcgac ctgctcaacg ccgagctcga
    16441 cggcaatccc atcgtcaccc gcgaccactg ctacgcctgc accgccggcc agggctcgag
    16501 ctgcggcggc gcgctcaagg cctgagcgtg gaccgccgcc tggcgctgct cggcctgctg
    16561 ctggcgctgg ctgcgctgtt cttcgtcctc gatctgggcc agtacctcga cctgtcccgg
    16621 ctgaaggcag agcaggcagc gctcgccgct ggggtcgccg cccatccctg gcgcgcggcg
    16681 gggctgttcg ccctggtcta tgtcgcggtg accgcgctgt cgctgccggg cgcggcgctg
    16741 ctgaccctgg tgggcggcgc gttgttcggc ctgctcgcgg gcaccgtgct ggtgtccctg
    16801 gcctccaccc tcggcgccag cctggccatg ctcggcagcc gcttcgtgct gcgcgactgg
    16861 gtgcaggcac gcttcggccc gcgcctggcc gccatcgacc gcggcctgga gcgcgacggt
    16921 gccttctacc tgttcgccct gcgcctggta ccggtctttc cgttcttcct gatcaacctg
    16981 gccatgggcc cgacccgcct gccgctgcgc accttcgcct gggtcagcca gctcggcatg
    17041 ctgccgggca cgctggtcta cgtgaacgcc ggccgcgagc tgggccggct ggacagcctg
    17101 gccgacatcc tctcccccgg cctgctcggc gccttcgccc tgctcggcct gttccccctg
    17161 ctggcgcgcc gggtgctgga cggactcaag gctcgcaggg tgtatgccgg ctggccgcgg
    17221 ccgcggcact tcgagcgcaa cctggtggtg atcggcgccg gttccggcgg cctggtcagc
    17281 gcctacctcg ccgccgccct gcaggcgcgg gtgaccctga tcgagaaggg ccagatgggc
    17341 ggcgactgcc tgaaccgcgg ctgcgtaccg tccaaagcgc tgctgcgctc ggcgcgggtc
    17401 gccagcgagc tgcgcaaggc cgcgc
//
LOCUS       DATCUN010000286         4993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_13985, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000286 DATCUN010000000
VERSION     DATCUN010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13985"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1692
                     /locus_tag="VIO83_11080"
     CDS             <1..1692
                     /locus_tag="VIO83_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY8331855.1"
                     /translation="PIVLYSAYHEAQASSGHSLARLIAQLLAFVGAMLMLVLASDLLT
                     LLIAWELVGALSWALIAHHWPSERSVRDAAWAFLVTRFGDLGLYVAAGAAFAGSGSFD
                     LAGLGRLDGMPLQVFVGGIVLACAAKSAQLPFSPWLFAAMSGPTPVSALLHAATMVAA
                     GVYLIIRLHPVLDGVTWFAPLAMALGLATAICGGIVASLQGHAKKLLAASTSAHYGLM
                     WLAVGAGFPGAALLHFVTHAFFKAGLFLAAGIAEHHVGSYSLGDMRLGRTLPGIALAT
                     LLSSLALAGVPPLGGAWSKEQLIGAAGHHAAWLALIAMLAGALSALYATRFQWMAYGP
                     AASDFTPPRARGPGHTPPEALALYVLAGGTLALSLLWWPGFAETLSDRLGIEMPPSKQ
                     WEILGSLLFVALGILLGALAILRHWAHRDEANRRFATVSDWLGIPPVAGRTASGLMNI
                     SHALARFDDRIVDAAIQRVASYRAFADRLGRFDDRAVDGGIRLTARFGAWLASFGNRR
                     GEALFDGLVDTVAQVTGWLGQRARQLQSGQMHHYYAAIAAGLAAVLIILAMGELP"
     gene            1689..3176
                     /locus_tag="VIO83_11085"
     CDS             1689..3176
                     /locus_tag="VIO83_11085"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY8331856.1"
                     /translation="MILTAAIGLPLAGALTLAAIPRWREAAARRFATAVALLSLLCLV
                     LAWRRFTPDGALFQLVEEVPWVPGLGMAWRLGVDGIALAVASMSALVFVASIMYPVDT
                     QHRAGPYYAWMLFLQGVSLGVFLALDLLLFYVFFDLSLVGMFFLIGRWGHGERQVSAL
                     KFFLYTFCGSLLMLLAFIGLYLSVAPHSFDMRVLIAEQPMVGSAIPAGLVFLGLMIGL
                     AIKTPLVPFHTWLPQAHVDAPAPASAILAGVLLKMGTFGMLRIPFAMMRETFAAYAWV
                     IALVAVISILYGASVALGQRNLKRRIAYTSINHMGYTVLGIAVAGALLSGNEAARQLA
                     LVGATVEMVAHGLITGSLFLICGSFWQRTGEYEMAQYGGLAKRAPLLTGFSVLAAFAS
                     LGLPGLAGFVAEFHVFAGTFSVYPWLAVIGIFGILITAALFLQTLQQLFFGELPQKWS
                     GFADLDRREWLVLGVLAAAFVIIGIAPQFLLGIIEASAGWLVGAR"
     gene            3227..4585
                     /locus_tag="VIO83_11090"
     CDS             3227..4585
                     /locus_tag="VIO83_11090"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY8331857.1"
                     /translation="MIVLASSFLPQQRLSWMAAVALLAIASAAGFSLDQWGEPARLTF
                     SGVWALDGPAIAAKLIMLAAGAVVVALSPGWLATDRRHGEFYALLLFALLGVVTLASA
                     SDTMELVVGMLLSSAASYPLAAFHRGFAPALEAGMKYFLVGALTNTLLCIGVVWLFGL
                     TGSTGYPAIAGRLAQGVDELALLTATGCIVVGLSFKLAAFPAHAWMPDVAQASPAPAA
                     AFLTVVPKVGAAIALARLLALLPAPHHPTLLIALLAAATMTLGNLAALWQKDVRRLLG
                     WSAVAQSGFALMAIAAMPTSPDARTSLLLFLATYAIANLAAFAVVIELRGRTTLKDYA
                     GLARSAPGPALVLILALLSLVGIPPLIGFFGKFMLYRSTLQGGLGWLAVVAALNSAVS
                     LFYYLRVAGTIVFGQPDGAVHRLGGASPALVWLSGALLLAGGLAVELLVDALQGVGSF
                     AG"
BASE COUNT          642 a         1670 c         1640 g         1041 t
ORIGIN      
        1 cccatcgtgc tatacagcgc ctaccatgaa gcgcaggcat cttccgggca ttcgctggcg
       61 cggctgatcg cgcagctgct ggccttcgtc ggcgccatgc tgatgctggt gctggcgagc
      121 gatctgctca cgctgctgat cgcctgggag ctggtcggcg cgctgtcctg ggcactgatc
      181 gcgcatcact ggccgagcga gcgttcggtg cgcgacgcgg cctgggcctt cctggtgacc
      241 cgcttcggcg atctcggcct gtacgtggcg gccggcgccg ccttcgccgg cagcggctcg
      301 ttcgacttgg ccgggctcgg gcgcctggat ggcatgccac tgcaggtgtt cgtcgggggc
      361 atcgtgctcg cgtgcgccgc caagtccgca cagcttccct tctcaccgtg gctgtttgcc
      421 gccatgtccg ggccgacgcc ggtttcggcg ctgctgcatg cggcaaccat ggtcgcagcc
      481 ggtgtctacc tgatcattcg tctgcatccg gtgctggacg gcgtgacgtg gttcgcgccg
      541 ctggccatgg cgctgggatt ggcgaccgcc atctgcggtg gcatcgttgc cagcctgcag
      601 ggccacgcga agaaactgct ggccgcctcg acctcggccc attacggctt gatgtggctg
      661 gcggtgggcg ccggctttcc cggtgctgca ttgctgcact tcgtcaccca tgcgtttttc
      721 aaggccggct tgtttctcgc cgcggggatc gccgagcacc atgtcggcag ttactcgctc
      781 ggcgacatgc gcctgggtcg aacactgccc ggcatcgccc tggcaacgct gctcagcagc
      841 ctggcattgg caggcgtgcc gccgctgggc ggtgcctgga gcaaggaaca actgataggt
      901 gcggcgggcc atcacgcggc ttggctggca ctgatcgcca tgctggcagg cgcactcagc
      961 gcgctctatg caacacgctt tcagtggatg gcgtacggcc cggcggcgtc cgacttcaca
     1021 ccaccgcgcg cacgcggtcc gggccatacc ccgccagagg cgctggcgct gtacgtgctc
     1081 gccgggggca cgctggcatt gtcgctgctc tggtggcccg gcttcgccga aacgctttcc
     1141 gaccgtttgg gcattgaaat gccacccagc aagcaatggg aaatccttgg ttcgctgctg
     1201 ttcgtggcgc tgggcatcct gctcggtgcc cttgcaatcc tcaggcactg ggctcaccgc
     1261 gacgaagcta accgccgttt tgcaacggtc agcgattggc tgggcattcc acccgtcgcc
     1321 gggcgcactg cgagcgggct gatgaacatc agccatgcac tggcgcgctt cgatgaccgg
     1381 atcgtagatg ccgccatcca gcgggtggcc agctatcgag catttgccga ccgcctcggt
     1441 cgcttcgatg accgggccgt agacggcggt attcgtctga ctgcccgctt cggcgcctgg
     1501 ttggccagct tcggcaaccg ccgcggcgaa gcgctgttcg atggcctcgt cgataccgtt
     1561 gcgcaagtca ccggatggct cggccaacgc gcccggcagc tgcagagcgg ccagatgcac
     1621 cactactacg ccgccatcgc cgccgggctg gcggcggtgt tgatcatcct tgccatggga
     1681 gaactgcctt gatactcacc gctgccatcg gcctgcccct ggcgggcgcg ctgactctgg
     1741 ccgccatccc acgctggcgc gaggccgccg cgcgccggtt cgccaccgcc gtggcgctgc
     1801 tttcgctgct ttgcctggtc ctcgcctggc gacgcttcac cccggacggc gcactgttcc
     1861 agctggtgga ggaagtgccc tgggtccccg gccttgggat ggcctggcgg ctcggcgtgg
     1921 acggcatcgc cctggcggtg gcgagcatga gcgcgctggt gttcgtcgcc agcatcatgt
     1981 atccggtcga cacccagcat cgcgccgggc cctactacgc ctggatgctg ttcctgcagg
     2041 gcgtgtcgct gggtgtgttc ctcgccctcg acttgctgtt gttctacgtg ttcttcgatc
     2101 tgtcgctggt gggcatgttc tttctcatcg gtcgctgggg gcacggtgaa cgccaagtct
     2161 cagcgctgaa gttctttctc tatacctttt gcggctcgtt gctgatgttg ctggccttca
     2221 tcgggctgta tctgtcggtg gcaccgcaca gcttcgacat gcgcgtgctg atcgccgaac
     2281 agcccatggt cggctcggcc attccggcgg ggctggtgtt cctcggcctg atgatcggcc
     2341 tggcgatcaa gacgccgctg gtgccctttc atacctggct gccgcaggcg cacgtggatg
     2401 ctccggcgcc ggcctcggcc atcctcgccg gtgtgctgct gaagatgggt accttcggca
     2461 tgctgcgcat tcccttcgcg atgatgcgtg aaaccttcgc cgcctacgcc tgggtgatcg
     2521 cgctggtggc ggtgatctcg atcctctacg gcgcctcggt ggcgctgggc cagcgcaacc
     2581 tgaagcggcg gatcgcctac acctcgatca accacatggg ttatacggtg ctcggcatcg
     2641 cagtcgccgg cgcgctgctc agcggcaacg aggctgcgcg gcaactggcg ctggtcggcg
     2701 cgaccgtgga gatggttgca catggcctga tcaccggcag cctgttcctg atctgcggct
     2761 cgttctggca gcgcaccggt gaatacgaga tggcgcaata cggcggcctg gccaagcgcg
     2821 cgccgttgct gaccggcttt tccgtgctcg ccgccttcgc cagcctcggc ctgcccggac
     2881 tggccggctt tgtggccgaa ttccacgtgt tcgccggaac gttttccgtt tatccgtggc
     2941 tggcggtcat cggcatcttc ggcattttga tcaccgccgc cctgttcctg cagaccttgc
     3001 agcagctttt tttcggcgag cttccgcaga agtggagcgg ttttgctgac ctcgatcgcc
     3061 gggaatggct ggtgctcggt gtgctggccg ccgcgttcgt catcatcggc attgcaccgc
     3121 agttcctcct cggcatcatc gaagccagcg cgggctggct ggtgggcgcg cgctgatgcc
     3181 agtcggggat ctgttaccgg agatatgcct gctactgggt gcagtggtga tcgtcctggc
     3241 ctcctcgttc ctgccgcagc aacgtctgag ttggatggcc gcggtcgccc tgctggcaat
     3301 cgccagcgcg gctggtttct ctctggacca atggggcgag cccgcgcggc tgaccttctc
     3361 tggtgtctgg gcgctggacg ggccggcgat cgcggccaag ctgatcatgc ttgcggccgg
     3421 cgccgtggtg gtggccctgt cgcccggctg gttggccacc gatcgccgcc acggcgagtt
     3481 ctacgccctg ctgctgttcg ccctgctcgg cgtggtcacg ctcgcctcgg ccagcgacac
     3541 gatggagctg gtggtcggca tgctgttgtc ttctgccgcc agttatccgc tggccgcgtt
     3601 tcatcggggc ttcgctccgg ccctggaggc ggggatgaag tatttcctcg tcggcgcgct
     3661 gaccaacacc ctgctgtgca tcggggtggt ctggctgttc ggcctgaccg gcagcaccgg
     3721 gtacccggca atcgcgggtc gactggcaca aggcgtcgac gagctggcgc tgctgaccgc
     3781 caccggctgc atcgtggtcg ggctgagctt caagctggcg gcctttcccg cccacgcctg
     3841 gatgcctgac gtggcccagg catcgcctgc cccggcggcg gcctttctca ccgtcgtgcc
     3901 caaggtgggt gcggcgatcg cattggcgcg gttgctggcc ctgttgcccg caccgcacca
     3961 cccgacgctt ctgatcgccc tgctcgccgc tgccaccatg accctcggca atctggccgc
     4021 gttgtggcag aaggacgtcc ggcgcctgct gggctggtcg gcggtggcgc agtccggctt
     4081 cgccctcatg gccattgcgg cgatgccgac cagccccgat gcgcgcacct cgctgctgct
     4141 gtttctggcg acctatgcca tcgcgaatct ggccgccttc gctgtggtga tcgagctgcg
     4201 cgggcgcaca accttgaagg actatgctgg cctcgctcgg tcagcgccag ggccagcgct
     4261 ggtgttgatc ctggcgctgc tgtcgctggt gggcatcccg ccgctgatcg gcttctttgg
     4321 caagttcatg ctctaccgaa gcaccctgca gggcggcctc ggctggctgg cggtggtcgc
     4381 cgcgctgaac agcgccgtct cgctgttcta ttacctaaga gtcgcaggca ccatcgtctt
     4441 cgggcagccc gacggagccg tccatcggct cggaggtgcg agccctgcgc tggtctggct
     4501 cagcggcgcg ttgctgttgg caggggggct ggccgtcgag ctgctggtcg acgcgctgca
     4561 aggcgtcgga agctttgcag gttagaccga tggcgcggac ggtgcagccg ccacgcggtc
     4621 ccgccgtcgg gacaacgacc caatgcggtt acgcagcctg tgggactttc caaaacatga
     4681 gccaggtacg tgaattctcg aagacttcgc gccctgtaaa tccatttcat gcacctatac
     4741 ggacgatttt ggggattttt aaccaatttc cggcccgccc attgaaagcc cgacaggctc
     4801 ctgataggcg ctttgagcag tatcactcac cgccttccgc acactgacgg acagttagtc
     4861 acggaaggcc cgctcgacca cctcgacgat atcggaccag atgcctggtt ctcgcaacac
     4921 cggcatttcc ttggccaaca cgttgctgag ctcccctcga ttctgctcga tcgaacgcaa
     4981 cacccggtcg gcc
//
LOCUS       DATCUN010000287         5448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_259030, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000287 DATCUN010000000
VERSION     DATCUN010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259030"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VIO83_11095"
     CDS             <1..420
                     /locus_tag="VIO83_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HEY8331858.1"
                     /translation="GNFALLAGIEPGQLCDWYLAVYMDAFDWVELPNVLGMVLHADGG
                     YLGSKPYCASGQYIKRMSDYCQGCAYQVSESTTGDACPFNALYWHFLMRHREQLQGNP
                     RLGMVYKNLARMDEAKRQALWQRGEALLARLDGGEAL"
     gene            417..545
                     /locus_tag="VIO83_11100"
     CDS             417..545
                     /locus_tag="VIO83_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HEY8331859.1"
                     /translation="MRKKADLPIKHCARCGLPFAWRKKWARCWDEVRYCSERCRRG"
     gene            713..1330
                     /locus_tag="VIO83_11105"
     CDS             713..1330
                     /locus_tag="VIO83_11105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY8331860.1"
                     /translation="MSQLDLSTDEARVSYGIGRQLGGQLRDNPPPGASLEAILAGLSD
                     AYNNADSRVPDEAMNASFQVIRERMQAEQAAKAEQAAQAGRDYLAENAKREGVTVLAS
                     GLQFEVLVAGEGAKPSAEDQVRTHYHGTLIDGTVFDSSYERGQPAEFPVGGVIPGWVE
                     ALQLMNAGSKWRLHVPSELAYGAQAIGSIPPHSVLVFDVELLDIL"
     gene            complement(1621..2589)
                     /locus_tag="VIO83_11110"
     CDS             complement(1621..2589)
                     /locus_tag="VIO83_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY8331861.1"
                     /translation="MQPQAFYRVVADDFTAVDEIIRRRLTSRVPLVAKIGDYIVSAGG
                     KRLRPLLVLLAGNALGQRGERLNLLAATIEFLHTSTLLHDDVVDKSSLRRGRSTANAL
                     WGNAPSVLVGDFLYARSFEMMVELGSLPVMQVISQATRVIAEGEVLQLSKVRDASTTE
                     EIYMEVIRGKTAMLFEASTHSAALLSGASAAQAEALRSYGDALGIAFQLVDDLLDYRG
                     DAATLGKNVGDDLAEGKPTLPLIYAMREGTPEQATLVRKAIQQGGIEDLEGILAAVEA
                     SGALDYTAQLARDYAARAIACLEALPPSEYRDALVELTEFAVARTH"
     gene            2831..3142
                     /gene="rplU"
                     /locus_tag="VIO83_11115"
     CDS             2831..3142
                     /gene="rplU"
                     /locus_tag="VIO83_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003154364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY8331862.1"
                     /translation="MYAVIVTGGKQYKVAEGEFLKIEKLDVATGESVAFDKVLLVGNG
                     DDVQIGAPVVAGAKVTAEVIAQGRHDKVTIIKFRRRKHHMKRQGHRQWFTEIKITGIQ
                     A"
     gene            3168..3425
                     /gene="rpmA"
                     /locus_tag="VIO83_11120"
     CDS             3168..3425
                     /gene="rpmA"
                     /locus_tag="VIO83_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY8331863.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKMYGSQAAVAGNILVRQRGTK
                     FHAGVGVGMGKDHTLFAKVAGVVKFETKGAFGRKYVSVVAN"
     gene            3522..4745
                     /gene="cgtA"
                     /locus_tag="VIO83_11125"
     CDS             3522..4745
                     /gene="cgtA"
                     /locus_tag="VIO83_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HEY8331864.1"
                     /translation="MKFVDEVSIHVQAGDGGNGCMSFRREKFVPNGGPDGGDGGDGGS
                     IWLEADSNLNTLVDYRYTRRFNAQRGQNGGSKDCTGSKGEDMTLPVPVGTTVIDAGTQ
                     EIIGDLTKDGQRLLVAMGGWHGLGNTRFKSSTNRAPRQTSHGKPGDARDLKLELKVLA
                     DVGLLGMPNAGKSTLIRAVSAAKPKVADYPFTTLVPNLGVVSVDRMKSFVIADIPGLI
                     EGAAEGAGLGIRFLKHLARTRLLLHLVDMAPLDESDPAEAAAAIINELGRFSPALTQR
                     DRWLVLNKADQLLDDEREERMREVIARLDWKGPVYVISALEREGTNQLCHDIMRYIDE
                     RTQRIAEDPAFAEAVVALDQQIEDEARARLQELDDARALRRSGVKSAATVEDEDDDFD
                     DDEDDGDGPEIIYVR"
     gene            4808..>5448
                     /gene="proB"
                     /locus_tag="VIO83_11130"
     CDS             4808..>5448
                     /gene="proB"
                     /locus_tag="VIO83_11130"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY8331865.1"
                     /translation="MREKVSKARRWVVKIGSALLTADGRGLDRDAMAVWVEQMVALRR
                     AGVELVLVSSGAVAAGMSRLGWSARPTTIHQLQAAAAVGQMGLIQAWESSFAEHELQT
                     AQVLLTHDDLSDRKRYLNARSTLRTLVDLGVIPVINENDTVVTDEIRFGDNDTLAALV
                     ANLVEADLLVILTDRDGMFDADPRHNPDAQLIFEARADDPALDAVAGGTGGALG"
BASE COUNT          958 a         1777 c         1856 g          857 t
ORIGIN      
        1 ggcaacttcg ccctgctcgc cggcatcgaa cccggccaac tgtgcgactg gtacctggcg
       61 gtgtacatgg acgccttcga ctgggtggag ctgcccaacg tgctgggcat ggtgctgcac
      121 gccgacggcg gttacctggg ctccaagccc tactgcgcca gcggccagta catcaagcgc
      181 atgtccgact actgccaggg ctgcgcctac caggtgagcg aaagcaccac gggcgatgcc
      241 tgcccgttca acgcgctgta ctggcacttc ctcatgcgtc accgcgagca gctgcagggc
      301 aatccgcggc tgggcatggt ctacaagaac ctggcgcgca tggacgaggc caagcgccag
      361 gcgctgtggc aacgcggcga ggcgctgctg gcgcgcctgg atggcgggga ggcgttgtga
      421 ggaagaaggc cgatctgcct atcaagcact gcgcgcggtg cggcctgccg ttcgcctggc
      481 gcaagaaatg ggcgcgctgc tgggacgagg tgcgttactg ctcggagcgc tgccggcgcg
      541 ggtaggcagg cgctcgcgaa cggtaggggc ccgcctgctg gcgatccgtg gtcgccagca
      601 gggcatcgcc agcaagcggg ctcctacaca ttctgtccca ggcaggtgcg tccccggcgg
      661 acgactgctg tgctagtctg gccggccatt tttccgcctc agtggagttc ccatgtccca
      721 actcgacctt tccaccgacg aagcccgcgt cagctacggc atcggccgtc agctcggtgg
      781 tcagctgcgc gacaacccgc cgcccggcgc cagcctggaa gccatcctgg ccggtctgtc
      841 cgacgcctac aacaacgccg acagccgcgt gccggacgag gcgatgaacg ccagcttcca
      901 ggtgatccgc gagcgcatgc aggccgagca ggccgccaag gctgagcagg ctgcccaggc
      961 cggtcgcgac tacctggccg agaacgccaa gcgcgaaggc gtcaccgtgc tggcctccgg
     1021 cctgcagttc gaggtgctgg tggcgggcga aggcgccaag ccgagcgccg aggaccaggt
     1081 gcgcacccac taccacggca ccctgatcga cggcaccgtg ttcgacagct cctacgagcg
     1141 cggccagccg gcggaattcc cggtcggcgg cgtgattccg ggctgggtcg aggccctgca
     1201 gctgatgaac gccggcagca agtggcgcct gcacgtaccg agcgagctgg cctacggcgc
     1261 ccaggcgatc ggcagcatcc cgccgcacag cgtgctggtg tttgacgtcg agctgctcga
     1321 catcctgtaa gcccgtccac gatctgctgc gcgtcggcca tgctgcgttg agatcggctt
     1381 cggacagtgg cctgccactg aacgcccgta agggcggccc cgaaggggcg agcggagcga
     1441 gtactgctca tttacacctc gtaaactccg cgtcctcagc cgatctcgcc ttgcctggtt
     1501 ctagctcgcg agatcgtgat caagcaccag gattgtcctt cccgcgccct tgcggcgcgg
     1561 gaaaacgaaa acgcccgtcg cggccaggcc ggacgggcgt tttgctgtgc gggtaataac
     1621 tcagtgggtg cgcgcgacgg cgaactcggt cagctccacc agcgcatcgc ggtactcgct
     1681 gggcggcagc gcctccaggc aggcgatggc gcgggccgca tagtcgcggg cgagctgggc
     1741 ggtgtagtcc agggcgccgg acgcttccac cgcggcgagg atgccttcga ggtcctcgat
     1801 gccgccctgc tggatggcct tgcgcaccag ggtcgcctgt tcgggcgtgc cttcgcgcat
     1861 ggcgtagatc agcggcaggg tgggcttgcc ttcggccagg tcgtcgccga cgttcttgcc
     1921 cagggtggcg gcgtcgccac gatagtcgag caggtcgtcg accagctgga aggcaatccc
     1981 cagggcgtcg ccgtagctgc gcagcgcctc ggcctgtgcg gcgctggcgc cggacagcag
     2041 cgcggcgctg tgggtggagg cctcgaacag catcgcggtc ttgccgcgga tgacttccat
     2101 gtagatctct tcggtggtgc tggcgtcgcg caccttggac agctgcagca cctcgccctc
     2161 ggcgatcacc cgggtggcct gggagatcac ctgcatcacc ggtagggagc ccagctcgac
     2221 catcatctcg aaggagcggg catacaggaa gtcgccgacc agcacgctgg gcgcgttgcc
     2281 ccacagggcg ttggcggtgg agcgaccgcg gcgcagcgag gacttgtcga ccacgtcgtc
     2341 gtgcagcagg gtcgaggtgt gcaggaactc gatggtggcg gccagcaggt tgaggcgctc
     2401 gccacgctgg ccgagggcgt tgccggccag cagcaccagc agcggacgca ggcgcttgcc
     2461 accggcggaa acgatgtagt cgccgatctt cgccaccagg ggtacgcggg aggtcaggcg
     2521 acgacggatg atctcgtcga cggcggtgaa atcgtccgcc accacgcggt aaaaggcctg
     2581 gggttgcatc agcggtcaag gctcctctaa ggttgcgcgg catgctaggt gcagggtctg
     2641 gcactgtcaa ggcgagccgg ggtcgcactt gcctgcccgc gcggctttgc gtacaatgcc
     2701 cggcccgaac ttccctggca ctccctgcct tacgcaattg catcgacgac ccttccggcg
     2761 cggtgcagcc atgctggcca ataactcctt cactaaagcg ccaggtgagc aggtctaacg
     2821 gagacattcc atgtacgcag taattgttac cggcggcaag cagtacaaag tcgctgaagg
     2881 cgaattcctc aagattgaaa agctcgacgt agcgaccggc gaaagcgttg ctttcgacaa
     2941 ggttctgctg gtcggcaatg gcgacgacgt acagatcggc gcgccggtcg tagcgggtgc
     3001 caaagtcacc gccgaagtga tcgcccaggg ccgtcacgac aaggtgacca tcatcaagtt
     3061 ccgtcgccgc aagcaccaca tgaagcgtca gggccaccgt cagtggttca ctgaaatcaa
     3121 gatcaccggc attcaggcct gatccctttt tctggagata ttgactcatg gcacacaaaa
     3181 aagctggcgg ttctacccgc aacggtcgcg actcagaagc taaacgcctt ggcgtgaaga
     3241 tgtacggtag ccaggctgca gtagccggca acatcctcgt gcgtcagcgc ggcaccaagt
     3301 tccacgccgg tgtcggcgtg ggcatgggca aggaccacac cctgttcgcc aaagtggcgg
     3361 gcgtggtcaa gttcgagacc aagggcgcct tcggtcgtaa atacgtgagc gtcgtggcca
     3421 actgaccaca agcttgctga agaagccctg tctgccgaca gggctttttt gtttgtacat
     3481 cccttgctct aataacccgc ccagggcggg aggcgttacc catgaaattc gtcgatgaag
     3541 tgtccatcca tgtgcaggcc ggtgacggtg gcaacggctg catgagtttt cgccgcgaaa
     3601 aattcgtgcc caatggtggt ccggatggcg gcgacggcgg tgacggcggc tcgatctggc
     3661 tcgaggccga cagcaacctc aacaccctgg tggattatcg ctacacccgc cgtttcaatg
     3721 cccagcgcgg gcagaacggc ggcagcaagg actgcaccgg gagcaagggt gaagacatga
     3781 ccctgccggt gccggtcggc accacggtga tcgatgccgg gacccaggaa atcatcggcg
     3841 acctgaccaa agacggtcag cggctgctgg tggcgatggg cggctggcac ggtctgggca
     3901 atacccgctt caagtccagc accaaccgcg cgccacggca gaccagccat ggcaagccgg
     3961 gcgatgcgcg cgacctcaag ctggagctca aggtgctggc cgacgtcggc ctgctcggca
     4021 tgcccaatgc cggcaagagc accctgatcc gcgcggtatc cgccgccaag cccaaggtcg
     4081 ccgactaccc gttcaccacc ctggtgccca acctcggcgt ggtcagcgtc gaccgcatga
     4141 agagcttcgt gatcgccgac attcccggcc tgatcgaggg tgccgcggaa ggtgccggcc
     4201 tgggcatccg cttcctcaag cacctggcgc gtacccgcct gctgctgcac ctcgtcgaca
     4261 tggcgccgct ggacgagtcc gatccggccg aagccgccgc ggcgatcatc aacgagctgg
     4321 ggcgcttcag tccggcactg acccagcgcg accgctggct ggtgctgaac aaggccgacc
     4381 agctgctcga tgacgagcgc gaggagcgca tgcgcgaagt gatcgcgcgg ctcgactgga
     4441 agggcccggt gtacgtgatc tccgcgctgg agcgcgaggg caccaaccag ttgtgccacg
     4501 acatcatgcg ctacatcgac gagcgtaccc agcgcattgc cgaggacccg gcgttcgccg
     4561 aggccgtggt cgcgctggat cagcagatcg aggacgaggc gcgtgcccgt ctgcaggaac
     4621 tcgacgacgc tcgtgcgctg cgccgcagcg gggtcaagag tgccgcgacg gtggaagacg
     4681 aggacgacga cttcgacgac gacgaggatg acggcgacgg gccggaaatc atctacgtcc
     4741 gctgacgtcc gtgtgacgcg gggccgcctg cggcccctga tcaagcctga ttgcaaggtt
     4801 gcagaacatg cgtgagaagg tgagcaaggc ccgccgctgg gtggtgaaga tcggcagcgc
     4861 gctgctgacc gccgacggcc gcggcctcga ccgcgacgcc atggcggtgt gggtcgaaca
     4921 gatggtggcg ctgcggcgcg ccggggtgga actggtgctg gtgtcctccg gggcggtggc
     4981 ggcggggatg agccggctgg gctggagtgc ccggccgacc accatccacc agctgcaggc
     5041 cgcggccgcg gtggggcaga tgggcctgat ccaggcctgg gaatcgagct tcgccgagca
     5101 cgaactgcag accgcccagg tgctgctgac ccacgacgac ctgtccgacc gcaagcgcta
     5161 cctcaacgcg cgcagcaccc tgcgcaccct ggtcgacctc ggcgtgatcc cggtgatcaa
     5221 cgagaacgac acggtggtca ccgacgagat ccgcttcggc gacaacgaca ccctggccgc
     5281 gctggtggcc aacctggtgg aagccgacct gctggtgatc ctcaccgacc gcgacggcat
     5341 gttcgacgcc gacccgcggc acaaccccga cgcccagctg atcttcgagg cgcgcgccga
     5401 cgacccggcg ctcgacgcgg tggccggcgg taccggcggc gccctggg
//
LOCUS       DATCUN010000288         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_69775, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000288 DATCUN010000000
VERSION     DATCUN010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69775"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..80
                     /locus_tag="VIO83_11135"
     CDS             <1..80
                     /locus_tag="VIO83_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HEY8331866.1"
                     /translation="LARDGGELAGHPTVASFAAIEPSAC"
     gene            251..1111
                     /gene="speE"
                     /locus_tag="VIO83_11140"
     CDS             251..1111
                     /gene="speE"
                     /locus_tag="VIO83_11140"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEY8331867.1"
                     /translation="MSDYQETLYDGYGQRFRIDQMLHEVRTEHQHLVIFQNARMGRVM
                     ALDGVIQTTEADEFIYHEMLTHVPILAHGLAKRVLIIGGGDGGMLREVAKHRSVEHIT
                     MVEIDGTVVDMCKQYLPNHSCGAFDDPRLNLVIDDGMRFVATTEEKFDVIISDSTDPI
                     GPGEVLFSENFYEACRRCLNDGGILVTQNGTPFMQLTEVQTTAKRMHGLFADWHFYQA
                     AVPTYIGGAMTFAWGSTNADYRKLPLETLRQRFAGSGIVTRYYNPEIHQGAFALPQYV
                     LQAIHKPSND"
     gene            complement(1310..1522)
                     /locus_tag="VIO83_11145"
     CDS             complement(1310..1522)
                     /locus_tag="VIO83_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEY8331868.1"
                     /translation="MSRYYIDCREHPGDVKCTVALAADTKEELLEAVVEHGRTVHGYA
                     DTPEFRNQVMQGMKEGTPPVETDKTA"
     gene            complement(1730..>2645)
                     /locus_tag="VIO83_11150"
     CDS             complement(1730..>2645)
                     /locus_tag="VIO83_11150"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY8331869.1"
                     /translation="SPAAMREGVARVRALTERPFNVNLFILDDPAPSDEEVARAQARL
                     DPLRAELGLPPGSRPARFCEDNRAQIETLLELAPPVVSFTFGILDAATLARFHQAGCL
                     VMGTATTVAEAQAWEAVGADLVCAQGGEAGGHRGTFLGDPQQACIGLMALLPQLAAAV
                     NIPVVAAGGIMDGRGIAASLLLGAQAAQLGTAFLCCPESGIPALWKEQILAARDDSTR
                     LTRAFSGRFARGIANDFMRRLAAEEDQVPAYPVQNALTGDIRQAATRQGRSEYLSLWA
                     GQGAAMARPLPAAALVQVLMTELADCRA"
BASE COUNT          430 a          914 c          870 g          431 t
ORIGIN      
        1 gtctggcccg cgacggcggc gagctggctg ggcaccccac cgtggccagc ttcgcggcta
       61 tcgagccgag cgcctgctga gtctgcggcc gtctgcccgc ccgtcgccgc ggcgggcgcg
      121 caggacggtg gcaatctcgg cgtcacctgg acgctgcaga ctgtcaccag gggcgattgc
      181 cgcctacaat tccaagtatc cgtggagcca gactcccgtt aattttcaac aacttacaaa
      241 ggaaaacgcc atgagcgatt accaggaaac actctacgac ggctacggtc agcgctttcg
      301 catcgaccag atgctgcacg aggtgcgcac cgagcatcag catctggtga ttttccagaa
      361 cgcccgcatg gggcgtgtga tggcgctgga cggcgtgatc cagaccaccg aggccgacga
      421 gttcatctac cacgagatgc tcacccacgt gccgatcctc gcccacggcc tggccaagcg
      481 cgtgctgatc atcggcggcg gcgacggcgg catgctgcgc gaagtggcca agcaccgcag
      541 cgtcgagcac atcaccatgg tcgagatcga cggcaccgtg gtcgacatgt gcaagcagta
      601 cctgcccaac cactcctgcg gcgccttcga cgatccgcgc ctgaacctgg tgatcgacga
      661 cggcatgcgt ttcgtcgcca ccaccgagga gaagttcgac gtcatcatct ccgactccac
      721 cgacccgatc ggtccgggcg aggtgctgtt ctcggagaac ttctacgagg cctgccgccg
      781 ctgcctgaac gacggcggca tcctggtcac ccagaacggc accccgttca tgcagctgac
      841 cgaagtgcag accaccgcca agcgcatgca cggcctgttc gccgactggc atttctacca
      901 ggcagcggta ccgacctaca tcggtggcgc catgaccttc gcctggggca gcaccaacgc
      961 cgactaccgc aagctgccgc tggaaaccct gcgccagcgc ttcgccggca gcggcatcgt
     1021 cacccgctac tacaacccgg aaatccacca gggcgccttt gccctgccgc agtacgtgct
     1081 gcaggcgatc cacaagccga gcaacgactg agggccaggc cccgctagcg cgagggcctt
     1141 ggagcaagcg ccaaagtctg gatgcgcaaa atagagaagg ggcctgacca ggccccttct
     1201 catttgcgga cgggaatgcc gccgtcgtct gcggtctatg ccttgaccct gcctcgtctt
     1261 gccgagggtt caggtcctga tgacggaggg tttcccggtg ggttgagggt cacgccgtct
     1321 tgtcggtttc gacgggcggt gtaccttcct tcatcccttg catgacctgg ttgcggaact
     1381 ccggagtgtc tgcgtagcca tgcacggtac gcccgtgctc gacgacagct tccagcagtt
     1441 cttccttggt atcggcggcc agtgccactg tgcacttcac gtcacccgga tgttctcggc
     1501 aatcgatgta gtacctggac atgatggcgc cctccagatc gctagggccc aactggcctg
     1561 cctccgatcc cgctagggtc ggggcagggc ctcggagtcg cccccgccgg gctttgttga
     1621 ccaaggggtg gcgaactctc tttttagtat agatcgccac tgcggttctc cagctcgccg
     1681 cgggaccttc cgccgaacgg tccatggcct gccgcgcccg cgcgtcagct caggcgcggc
     1741 agtccgccag ctccgtcatc agcacctgta ccagtgcggc cgccggcagc gggcgagcca
     1801 tggcggcgcc ctggccggcc cacagcgaca ggtactcgct gcgcccctga cgggtggcgg
     1861 cctggcggat gtcgccggtc agtgcgttct gcaccgggta ggccggcacc tggtcttcct
     1921 cggccgccag gcggcgcatg aagtcgttgg cgatgccgcg ggcgaagcgg ccggagaagg
     1981 cgcgggtcag ccgcgtgctg tcgtcgcggg cggcgaggat ctgctccttc cacagcgcag
     2041 gaatgcccga ttccgggcaa cagaggaagg cggtgcccag ctgcgcggcc tgggcgccga
     2101 gcagcaggct ggcggcgatg ccgcggccgt ccatgatgcc gccggccgcc accaccggaa
     2161 tgttcaccgc cgcggccagc tgcggcagca gggccatcag gccgatgcac gcttgctgcg
     2221 gatcgccgag gaaggtcccg cgatggccgc cggcctcgcc gccctgggcg cataccaggt
     2281 cggcaccgac cgcttcccag gcctgcgctt ccgccacggt ggtggcggtg cccatcacca
     2341 ggcagccggc ctggtggaag cgcgccagcg tggctgcatc gaggatgccg aaggtgaagc
     2401 tgaccaccgg cggggccaac tccagcaagg tctcgatctg tgcgcggttg tcctcgcaga
     2461 agcgcgccgg ccggctgccg ggcggcaggc ccagctcggc acgcagcggg tcgagtcggg
     2521 cctgggcgcg agccacttcc tcgtcactgg gcgcgggatc gtcgaggatg aacaggttga
     2581 cgttgaaggg gcgctcggtc agcgcgcgca cgcgggccac gccctcgcgc atcgctgccg
     2641 gcgac
//
LOCUS       DATCUN010000289        17276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_164738, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000289 DATCUN010000000
VERSION     DATCUN010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 17276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17276
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164738"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /gene="argA"
                     /locus_tag="VIO83_11155"
     CDS             <1..638
                     /gene="argA"
                     /locus_tag="VIO83_11155"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEY8331870.1"
                     /translation="PQQVPAHLARLGSDYQGELLDAAAQACRGGVRRSHIVSYGQDGA
                     LLNELFTREGAGTLVAQEQFEQVREADINDVGGLLDLIRPLEEQGILVRRSREVLERE
                     IGQFSIVEREGLIIACAALYPVPDSDWGELACLAVHPEYRQGGRGDELLERIEQRARA
                     QGLKTLFVLTTRTAHWFQERGFKPCSVERLPAARASLYNFQRQSKVFEKPL"
     gene            complement(797..1798)
                     /locus_tag="VIO83_11160"
     CDS             complement(797..1798)
                     /locus_tag="VIO83_11160"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEY8331871.1"
                     /translation="MSSEGNRRYFMVRTEASDVRKDRVAVGWRQADFSQFNSGQDLLR
                     HIRAHYGPIRSARQAERFFEMREGDVVVSPLSGSIAIGVVSSSECQFDSASSQAYPNV
                     RRVHFRRQEGQPVRIPRSLLSTALQSRLRAPGLIVSELKSFASELEELLNKDPDFSFR
                     NRAEQRNSELAETFKSELLERIQSGQTHLKGGGLGLEKLVRQLLETQGYQARSLGTRG
                     FDGKADADIEAVKDDSFSTTRLLVQVKHHQGETGSWGIEQLDKIRADSRYDDCDMVLV
                     SSGSFSETTAAHAYERNISLIGGCRLVEIILENASALPADTLHELGISSVPQLLPRT"
     gene            complement(1974..3551)
                     /gene="gshA"
                     /locus_tag="VIO83_11165"
     CDS             complement(1974..3551)
                     /gene="gshA"
                     /locus_tag="VIO83_11165"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY8331872.1"
                     /translation="MSDLLSRRLALLDSAEQRDLLRHCRHGLEKESLRVDPAGHLALT
                     PHPQALGSALTHAQITTDYSEALLEFITAAEEDPTRSLDELDAIHRFAYANLGEEKLW
                     THSMPCQLPAEAEIPIAEYGSSHMGQIKHVYRKGLAVRYGKAMQCIAGIHYNFSLPEA
                     LWPLLQAAEGDTSPAQDYQSERYVALIRNFRRYSWLLMYLFGASPAVCASFLKDREHH
                     LETLGEGTLYLPWATSLRMSDLGYQNNAQASLRPCYNDLDSYLASLRQAVSTPYPPYE
                     AIGLQRDGEWQQLNTNVLQIENEFYSSIRPKRVANAGERPIQALGARGVQYVEVRCLD
                     IDPFLPLGIDLIAARFLDAFLLFCALEDSPSMPGDECRECTANFALTVKEGRRPGLQL
                     SRHGEPRELKAWGLELLERLATCAALLDRTHGGDLHQQALNAQRAKLQDASLTPSARV
                     LRSLREDHLSFFAFAQRQSERHAAWFRGRPLDAASEAAFAQSARDSLAAQAKLEREQQ
                     GSFEEFVEHYMAAIEPS"
     gene            complement(3769..4152)
                     /locus_tag="VIO83_11170"
     CDS             complement(3769..4152)
                     /locus_tag="VIO83_11170"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012316725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY8331873.1"
                     /translation="MTDVREVTTSAYSHLLGCELTRLDADQAEVRLAMRPELRNRAGK
                     LHGGAIFSLVDIAMGLACSSAHGFDRHSVTVECKINYMRAIDAGCVECLAKVIHYGSR
                     TLVVEAEVRQQDALVARAQGTFMLV"
     gene            complement(4154..6481)
                     /locus_tag="VIO83_11175"
     CDS             complement(4154..6481)
                     /locus_tag="VIO83_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HEY8331874.1"
                     /translation="MDSINARIAEELGVRPQQVAAAVALLDEGSTVPFIARYRKEVTG
                     SLDDTQLRTLEERLRYLRELDERRASILSSIEEQGKLTPELAREIKLADTKTRLEDLY
                     LPYKQKRRTKGQIALEAGLGELADALFADPALDPEREAARFVDAEKGFADVKAVLEGA
                     KYILMERFAEDADLLAKLRGFLKDNATLSARLVAGKEQEGAKFSDYFEHDELLKGVPS
                     HRALAIFRGRNEGILSASLKVGEELPGSMHPCEGMIAERFGIEARGRAADKWLAEVVR
                     WTWKVKLYTHLETDLLGELREAAESEAINVFARNLHDLLLSAPAGPRCTLGLDPGLRT
                     GCKVAVVDATGKLLDTATVYPHAPRNDWDGTLAVLARLCAKHQVELIAIGNGTASRES
                     DKLAADLIKQVPGLKLTKVMVSEAGASVYSASELAAREFPDLDVSIRGAVSIARRLQD
                     PLAELVKIEPKAIGVGQYQHDVSQLKLARSLDAVVEDCVNAVGVDVNTASAALLARIS
                     GLNATLAQNIVQFRDANGAFASRAALKKVPRLGDKTFELAAGFLRVMNGDNPLDASAV
                     HPETYPLVKRIAADTGRDIRSLIGDSGFLKRLDPAKFTDESFGLPTVTDILKELDKPG
                     RDPRPEFKTAEFQEGVESLKDLVPGMMLEGVVTNVTNFGAFVDIGVHQDGLVHISALS
                     EKFVKDPYEVVKAGDIVKVKVMEVDIPRNRVGLSMRMSDTPGEKVDGTRSGPRGGGKG
                     GNAPRSERHSSQDKPAPSNPGMAALFANAKQIKKR"
     gene            6660..7403
                     /gene="ompR"
                     /locus_tag="VIO83_11180"
     CDS             6660..7403
                     /gene="ompR"
                     /locus_tag="VIO83_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEY8331875.1"
                     /translation="MNSTASSQPEGEKILIVDDDVRLRRLLERFLAEQGYRVRTVENV
                     EQMDRLLSRELFNLVVLDLMLPGEDGLSACHRLREQGNAMPIIMLTAKGDESSRIQGL
                     EQGADDYLAKPFNPRELLARIRAVLRRQAPLVPGAPGSEEETVTFGDYELNLGTRELS
                     RGGEVHMLTTGEFAVLKALVQHAREPLTRDKLMSLARGREWDALERSIDVQISRLRRL
                     IEPDPSKPRYIQTVWGVGYVFVPDGSARK"
     gene            7400..8713
                     /locus_tag="VIO83_11185"
     CDS             7400..8713
                     /locus_tag="VIO83_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8331876.1"
                     /translation="MRTPLWFPQSVFARILWLVLIVVLFSKALTLVYLLMNQDMLVDR
                     QYSHGTALTVRAYWAADESSRAAIAEAAGLRQLRQDEVPQGEYHWPYTEVLEEQLRLE
                     LGEATELRMRAQRSPKLWVLAPALGPDWVEVPLYPYPLKGQRIWSVLGWFLAIGLLST
                     AAAWLLVRQLSLPLKRLVFAARQIGRGRSVRLPVGDTPSEMTELYRAFNQMSADVEQA
                     ARERELMLAGVSHDLRTPLTRLRLSLELLPADDELTEDMVRDIEDMDAILDQFLAFIR
                     DGRDEPLEELDLNELIREVAAPLNQSEERVRLCLEPLPDMPLRRVSIKRLLANLMGNA
                     LRHGGSGVEVCSSLSGGAAAPYVVLSVLDRGPGIDPAELETIFNPFIRGDRARSTQGT
                     GLGLAIVKRIAAQHGGSVELRNRSGGGVEARVCLPLGLLLPRDAA"
     gene            complement(8885..9790)
                     /gene="rimK"
                     /locus_tag="VIO83_11190"
     CDS             complement(8885..9790)
                     /gene="rimK"
                     /locus_tag="VIO83_11190"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HEY8331877.1"
                     /translation="MKIAVLSRNPRLYSTRRLVEAGQQRGHEMLVIDTLRAYMNIASH
                     KPQIHYRGQSLEGFDAVIPRIGASVTFYGCAVLRQFEMMGVFPLNESVAISRSRDKLR
                     SLQLLSRKGIGLPVTGFAHSPDDIPDLIRMVSGAPLVIKVLEGTQGIGVVLCETEKAA
                     ESVIEAFMGLKQNIMVQEYIKEAGGADIRCFVVGNKVIAAMKRQAKPGEFRSNLHRGG
                     SASLIKITPEERMTAIRAAKVMGLGVAGVDILRSNHGPLVMEVNSSPGLEGIETTTGQ
                     DVAAMIIQHIEEHARPNQTRTKGRG"
     gene            complement(9787..9948)
                     /locus_tag="VIO83_11195"
                     /pseudo
     CDS             complement(9787..9948)
                     /locus_tag="VIO83_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
     gene            complement(10120..11550)
                     /locus_tag="VIO83_11200"
     CDS             complement(10120..11550)
                     /locus_tag="VIO83_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023098450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEY8331878.1"
                     /translation="MKRFIQGEHRGQSTLLPESLDDYVADTNPVRVVDVFVDELDLGT
                     LGFDGVVPAETGRPAYHPADLLKIYIYGYLNRLQSSRRLEREAQRNVELMWLTGRLMP
                     DFKTIANFRKDNGKAIRGVCRQFVVLCQQLGLFSEALVAIDGSKFKAVNNRDRNFTSA
                     KLQRRMEEIESSINRYLTALDTADRQEPAVAQVKAERLHDKIATLKTKLQELKEIEVR
                     LNETPDKQISLTDPDARSMKTRGTGMVGYNVQAAVDANHHLIVTHEVTNNGVDRDQLS
                     SMAKQAREAMGVEELSAVADRGYFKGEEILACHEAGITVYVPKTLTSGATAAGRFGKG
                     DFIYDAATNEYRCPAGQSLIWRYSSVEKGLKLHRYWSSHCQGCALKEQCTPSPQRRVS
                     RWEHEAVLEAMQSRLDQAPEMMRIRRQTVEHPFGTLKSWMGATHFLTRTLDRVSTEMS
                     LHVLAYNLKRVLRLMGSDALMAAMKA"
     gene            complement(11623..11940)
                     /locus_tag="VIO83_11205"
                     /pseudo
     CDS             complement(11623..11940)
                     /locus_tag="VIO83_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
     gene            complement(12004..13485)
                     /locus_tag="VIO83_11210"
     CDS             complement(12004..13485)
                     /locus_tag="VIO83_11210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015275707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8331879.1"
                     /translation="MSEARKRLQQTRPPHRAGKRAGLPAGWHWLAALLLLAGLPLAAA
                     AQAVALTDSERAYLQVHPAVPLCVDPDWWPFEVIDPAGRHAGISADLIALVTQRTGVN
                     LQLQPRRNWEESLAASRSGDCLALSFLNSTPEREQWLIFTEPLLVDPNVIITREEHPF
                     VSDIGALQGKSIALPRGTAMAERIARDFPNLRIVPTDSEREALALVSERKADLTLRSL
                     IVVAATIKHEGWFNLKISGQIPGYDNQLRMGVLKSETTLRAILDRGIATLTPLERRQI
                     VDRHVNLTVVTDVVTDYTLAIWLGIVLAAILVTSLAWMARLRKLNRQLRSMAETDLLT
                     GLPNRHAIDSSFALDILRAQRYCRPLSVILLDIDFFKSVNDDLGHVMGDKVLVEFARL
                     LRDGLRNVDSGCRWGGEEFLVICHETTLEQAATLAERILQRVREHGFPCRRPLTASAG
                     VATVMPGDTVVALVQRADDALYDAKHSGRDRVALNLAALADVR"
     gene            13629..14018
                     /locus_tag="VIO83_11215"
     CDS             13629..14018
                     /locus_tag="VIO83_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEY8331880.1"
                     /translation="MSEKDDQARLDKWLWAARFFKTRALAKAAIEGGKVHCRGERCKP
                     SKEPRVGDELLIRSGFDERTVIVRVLSMVRRGAPEAQQLYEETADSIARREAAAALRK
                     AGALGVQSDGRPTKKQRRQIHQFRDGQ"
     gene            14131..15018
                     /gene="hslO"
                     /locus_tag="VIO83_11220"
     CDS             14131..15018
                     /gene="hslO"
                     /locus_tag="VIO83_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEY8331881.1"
                     /translation="MSDFSQRFLFEDTDVRGELVELDQSYAEVLAKHAYPEPVAQLLG
                     EMLAAVTLLSDILKYDGLLVLQARSAGPVPLLMVECSSEREIRGIARYDAEALGTASS
                     LRELMPEGVLAMTVDPKVGQRYQGIVSLDSETLSDCLSDYFALSQQLPSRFWLNADGR
                     RARGLLLQQLPADRLTDGEERAASWEHLVTLADTLTAEELLGLDNETLLYRLYHQEVV
                     RVFDAEPLRFGCSCSRERSANALVSLGEADALQLVEEQGGAVHIDCQFCNQRYPFDAA
                     DIAQLFAGGGSDAPSPTKH"
     gene            15153..16697
                     /locus_tag="VIO83_11225"
     CDS             15153..16697
                     /locus_tag="VIO83_11225"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEY8331882.1"
                     /translation="MTQANTAVYTDISVAELIEQAIQRGEGELAANGSLVVRTGHRTG
                     RSPMDRYIVEESSTQAKIAWGKINRPFPEDKFAALWDRVEAYLGERSHFVSHVHVGSA
                     EEHYLPVKMSTETAWHNLFGRCLFINPQTFNPAGKGEWRILNVPTFVCEPERDGTNSD
                     GCVILNFAEKKVLIAGMRYAGEMKKAMFSVQNFLLPEKDVLPMHCAANIGEEGDVTLF
                     FGLSGTGKTTLSADPSRYLIGDDEHGWGEGVVFNIEGGCYAKCIDLSEKNEPVIWSAI
                     QFGAVLENVVLDERTRQPDYADASLTQNSRAAYPLELVEKRSEKNLGGEPNAVIFLTC
                     DLTGVLPPVSILNNEQAAYHFLSGYTALVGSTEMGSGSGIKSTFSTCFGAPFFPRPAG
                     EYAELLIKRIKAFGSKVYLVNTGWTGGGYGVGKRFNIPTTRAVIAAIQSGALVGAETE
                     HLPIINLDVPKAVAGVDTELLNPRNTWADKSQYDQAARALAALFVDNFKKFEVSDAIR
                     SAGPQL"
     gene            complement(16848..>17276)
                     /locus_tag="VIO83_11230"
     CDS             complement(16848..>17276)
                     /locus_tag="VIO83_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HEY8331883.1"
                     /translation="LVMGSRADRWEQRGDKQLELVHAQLILLALLMLRGPQTLGELLA
                     RSTRLHAFDDQEEVRHHLERLMVRELACQLPRQPGQREERYAHQLGDPAELENLLAAR
                     SEEPAASTGLSNAQEERLATLEARLAELEARLARLEAGTD"
BASE COUNT         2727 a         5544 c         5949 g         3056 t
ORIGIN      
        1 gtccgcagca ggtgccggcg catctggcgc gcctgggcag cgactaccag ggcgagctgc
       61 tggatgcggc ggcccaggcc tgccgcggcg gcgtgcggcg cagccacatc gtcagttacg
      121 gccaggacgg cgcgctgctc aacgagctgt tcacccgcga gggcgccggc accctggtgg
      181 cccaggagca gttcgagcag gtgcgcgagg cggacatcaa cgacgtcggt ggtctgctcg
      241 acctgatccg cccgctggag gagcagggca tcctggtgcg ccgctcgcgc gaggtgctgg
      301 agcgtgagat cggccagttc agcatcgtcg agcgcgaagg gctgatcatc gcctgcgccg
      361 cgctctaccc ggtgccggat tccgactggg gcgaactggc ctgcctggcc gtgcaccccg
      421 agtaccgcca gggcggccgc ggcgacgagc tgctcgagcg catcgagcag cgcgcgcgcg
      481 cccagggcct gaagaccctg ttcgtgctca ccacccgcac cgcgcactgg ttccaggaac
      541 gcggcttcaa gccctgcagc gtcgaacgcc tgccggcggc gcgcgcctcg ctgtacaact
      601 tccagcggca gtcgaaggtg ttcgagaagc cgttgtaagg gagcggaaac gacaaagccc
      661 atgccttggc atgggctttg tcgtctgtga agcagcgtag ggtagaagac tcgcgcagcg
      721 atcttctacc aaggtttcgc gccatcggtg acgggcaaat cgctggtgcg agttgtccgt
      781 gagttgagtg agaggatcag gtgcggggta gcagttgcgg gacgctgctg atgcccagtt
      841 catgtagggt gtcggcgggc agggcgctgg cgttctcgag aatgatctcc accagccggc
      901 agccaccgat cagcgagata ttccgctcgt aggcatgggc ggcggtcgtc tcactgaaac
      961 tgccggaact gacaagaacc atgtcgcagt catcgtagcg tgagtccgcg cggatcttgt
     1021 ccagttgctc gataccccaa ctaccggttt cgccctgatg atgcttgacc tgtaccagta
     1081 aacgggtggt cgagaaactg tcgtccttca cggcctcgat atctgcatcg gccttgccgt
     1141 cgaagccgcg ggtaccgagg ctgcgggctt gatagccctg cgtctctaga agttgtctga
     1201 ccagtttctc cagaccgaga ccgcctccct tgaggtgtgt ttggcctgac tgaatgcgtt
     1261 ccagcagttc gctcttgaag gtctcagcca gctcgctatt acgttgttct gcacgattgc
     1321 gaaagctgaa gtcgggatct ttgttgagca attcctccaa ctccgaggca aagctcttca
     1381 gctcgctaac gatcaatccc ggtgcccgca gtcggctctg tagagcagtg cttagcaggc
     1441 tgcgtggaat ccgaaccggt tgaccctcct ggcgccgaaa atgaactcgc cgcacgttcg
     1501 gataggcttg gctacttgct gagtcgaact ggcactccga actgctgacc acgccgatag
     1561 caatgctacc cgacaacggc gacacgacga catcgccttc gcgcatttcg aaaaagcgct
     1621 cagcctggcg ggcgctgcga atggggccat agtgagcgcg gatgtgccgg agcaggtctt
     1681 gtcctgaatt gaactggctg aagtcggctt gtctccagcc aacggccaca cgatcttttc
     1741 gaacatcgct ggcctcagtg cggaccatga agtaacggcg gtttccttcc gaactcatcg
     1801 agagtttcct tgagggcatt ggtttaacgg tctggtgcgt tgcaggttac cgtttacctg
     1861 cgacagatca tgtcgctgcg tagtgtcatt gagtagagag ggcgggcttt tggcgatctt
     1921 ccagtatcgg caccggatga tcgccagcaa gctggctcct acagagtgag gcgtcagctc
     1981 ggctcgatcg ccgccatgta gtgttcgacg aactcctcga agctgccttg ctgctcgcgt
     2041 tccagcttgg cctgggcggc cagcgagtcg cgggcggact gggcgaaggc ggcttcgctg
     2101 gcggcgtcca gcggccggcc gcggaaccag gcggcgtggc gttcggactg gcgctgggcg
     2161 aaggcgaaga agctgagatg gtcctcgcgc aggctgcgca gcacccgcgc cgacggggtc
     2221 aggctggcat cctgcagctt ggcgcgctgg gcgttcagcg cctgctggtg caggtcgccg
     2281 ccgtgggtgc ggtcgagcag ggcggcgcag gtggccaggc gctcgagcag ttccaggccc
     2341 caggccttga gttcgcgcgg ctcgccgtgg cggctcagct gcaggcccgg gcggcgacct
     2401 tccttgacgg tcagggcgaa gttggcggtg cattcgcggc actcgtcgcc cggcatgctc
     2461 gggctgtctt ccagcgcgca gaacagcagg aaggcgtcga ggaagcgcgc ggcgatcagg
     2521 tcgatgccca gcggcaggaa cggatcgatg tccaggcagc gcacttcgac gtactgcacg
     2581 ccgcgcgcgc cgagggcctg gatcggccgc tcgccggcgt tggccacgcg cttgggacgg
     2641 atgctcgagt agaactcgtt ttcgatctgc agcacgttgg tgttgagctg ctgccactcg
     2701 ccgtcgcgct gcaggccgat ggcctcatag ggcgggtagg gcgtcgacac cgcctggcgc
     2761 aggctggcca ggtagctgtc caggtcgttg taacagggcc gcaggctggc ctgagcgttg
     2821 ttctggtagc ccaggtcgct catccgcagg ctggtcgccc agggcaggta cagggtgccc
     2881 tcgccaaggg tttccaggtg gtgttcgcga tccttgagga agctggcgca caccgccggc
     2941 gaggcgccga acaggtacat cagcagccag ctgtagcggc ggaagttgcg gatcagcgcg
     3001 acgtagcgct ccgactggta gtcctgcgcc gggctggtat cgccttccgc ggcctgcagc
     3061 agcggccaca gcgcctcggg cagggagaag ttgtagtgga tgccggcgat gcactgcatg
     3121 gccttgccgt agcgcaccgc caggcccttg cggtagacgt gcttgatctg gcccatatgg
     3181 ctgctgccgt attcggcgat ggggatctcg gcctcggccg gcagttggca cggcatcgag
     3241 tgggtccaca gtttctcctc gcccaggttg gcgtaggcga agcggtggat ggcgtccagc
     3301 tcgtcgaggc tgcgcgtcgg gtcttcctcg gcggcagtga tgaactccag cagcgcctcg
     3361 gaatagtcgg tggtgatctg cgcatgggtc agcgccgagc ccagggcctg cgggtgcggc
     3421 gtcagcgcca ggtggccggc ggggtcgacg cgcaggcttt ccttctccag gccgtggcgg
     3481 cagtggcgca gcaggtcgcg ctgttcggca ctgtcgagca gagccaggcg gcgggaaagt
     3541 agatcgctca aaagtcggat tcctgttcgc agagtcttcg cactatgggg gcgacaggcg
     3601 gcagtctcaa ggggcaggag cgtgttgtgc tgccgatgta gtcgaagggt agaagactcg
     3661 cgcagcgatc ttctaccgat gcggcgtgcc accggtggtg gacaatcgct ggcgcgagtt
     3721 gtccaccctg ggcagctacc ctacggctac cggcgctctt tcggcagcct agaccagcat
     3781 gaaggtgccc tgggcgcggg cgaccagcgc gtcctgctgg cgcacctcgg cttctaccac
     3841 cagggtgcgg ctgccgtagt ggatcacctt cgccaggcac tccacgcagc ccgcgtcgat
     3901 ggcgcgcatg tagttgatct tgcactcgac ggtgacgctg tggcggtcga agccgtgggc
     3961 gctggagcag gccaggccca tggcgatgtc gaccaggctg aagatcgccc cgccgtgcag
     4021 cttgccggca cggttgcgca gctccggacg catggccagg cgcacctcgg cctggtcggc
     4081 gtccagccgg gtcagttcgc agccgagcag gtggctgtag gcgctggtgg tcacttcgcg
     4141 gacgtcggtc atttcagcgc ttcttgatct gcttggcgtt ggcgaacagt gcggccatgc
     4201 ccggattgct gggggccggc ttgtcctggc tggagtggcg ctcgctgcgc ggcgcgttgc
     4261 cgcccttgcc gccgccacgc ggaccactgc gtgtgccgtc taccttctcg cccggggtgt
     4321 cggacatgcg catggacagg ccgacgcggt tgcgcgggat gtccacctcc atgaccttga
     4381 ccttgacgat atcgccggct ttcaccactt catacgggtc cttgacgaac ttctcggaca
     4441 gcgcgctgat gtgcaccagg ccgtcctgat gcacgccgat gtcgacgaag gcgccgaagt
     4501 tggtgacgtt ggtcaccacg ccctcgagca tcatgccggg caccagatcc ttgaggctct
     4561 cgacgccttc ctggaactcg gcggtcttga actccgggcg cgggtcgcgg ccgggcttgt
     4621 cgagttcctt gaggatgtcg gtgacggtgg gcaggccgaa gctctcgtcg gtgaatttcg
     4681 ccgggtcgag acgcttgagg aagcccgagt cgccgatcag cgagcggatg tcgcggccgg
     4741 tgtcggcggc gatgcgcttg accagcggat aggtctccgg gtgcaccgcc gaggcgtcca
     4801 gcgggttgtc gccgttcatc acgcgcagga agccggcggc cagttcgaag gtcttgtcgc
     4861 ccaggcgcgg caccttcttc agcgcggcgc gcgaggcgaa ggcgccgttg gcgtcgcgga
     4921 actgcacgat gttctgcgcc agggtcgcgt tgaggccgga gatgcgtgcc agcagggcgg
     4981 cggaggcggt gttcacatcc acgccgacgg cgttcacgca gtcctcgacc accgcgtcca
     5041 gcgaacgcgc cagcttgagc tgcgacacgt cgtgctggta ctggccgacg ccgatggcct
     5101 tgggctcgat cttcaccagc tcggccagcg ggtcctgcag gcggcgggcg atggacaccg
     5161 cgccgcggat cgacacgtcc agatcgggga attcgcgggc ggccagctcg gaggcggaat
     5221 acaccgaagc gccggcctcg gagaccatga ccttggtcag cttgagaccg gggacctgct
     5281 tgatcaggtc ggcggccagc ttgtcgctct cgcggctggc ggtgccgttg ccgatggcga
     5341 tcagctcgac ctggtgcttg gcgcacaggc gggcgagcac ggcgagggtg ccgtcccagt
     5401 cgttgcgcgg cgcgtgcggg tagacggtgg cggtgtccag cagcttgccg gtggcatcca
     5461 ccacggcgac cttgcagccg gtgcgcaggc cagggtcgag gccgagggtg cagcgcgggc
     5521 cggccggcgc gctgagcagc aggtcgtgca ggttgcgggc gaacacgttg atcgcctcgc
     5581 tctcggcggc ctcgcgcagc tcgccaagca ggtcggtttc caggtgggtg tacagcttga
     5641 ccttccaggt ccagcgcacc acctcggcca gccacttgtc ggcggcgcgg ccgcgggcct
     5701 cgatgccgaa gcgctcggcg atcatccctt cgcacgggtg catgctgcca ggcagttcct
     5761 cgccgacctt gaggctggcg ctgaggatgc cctcgttgcg gccgcggaag atcgccagcg
     5821 cgcggtgcga cggcacgccc ttgagcagct cgtcgtgctc gaagtagtcg ctgaacttgg
     5881 cgccttcctg ctccttgcca gcgaccaggc gcgcgctgag ggtggcgttg tccttgagga
     5941 agccgcgcag cttggccagc aggtcggcat cctcggcgaa gcgttccatg aggatgtact
     6001 tggcgccttc cagcaccgcc ttgacgtcgg cgaagccctt ctcggcgtcg acgaagcgcg
     6061 ccgcttcgcg ctccgggtcc agcgccgggt cggcgaacag cgcgtcggcc agctcgccga
     6121 ggccggcttc cagggcgatc tggcccttgg tgcggcgctt ctgcttgtac ggcaggtaga
     6181 ggtcttccag gcgcgtcttg gtgtcggcca gcttgatctc gcgggccagc tcgggggtca
     6241 gcttgccctg ctcctcgatg ctggacagga tcgaggcgcg gcgctcgtcc agctcgcgca
     6301 ggtagcgcag gcgctcttcg agggtgcgca gctgggtgtc gtcgaggctg ccggtgactt
     6361 ccttgcggta gcgggcgatg aagggcacgg tcgagccttc gtcgagcagc gccacggcgg
     6421 cggcgacctg ttgcgggcgg acgccgagtt cctcggcgat gcgggcgtta atgctgtcca
     6481 tatgacttcc tgctgcaagc gggggaagag ggccgggtgg ccgcaaaggg ccggattata
     6541 cccagcggcc cggttctggc gaggcgcagg gccggtcagg ggaaaaatct gctaacaatg
     6601 gccgcacatg gcgctgccgc gcctgcgcca taatcggccg atatttccga ggactcccga
     6661 tgaacagcac cgccagcagc cagccggaag gcgaaaaaat cctcatcgtc gacgacgacg
     6721 tgcgtctgcg ccgcctactc gagcgctttc tcgccgagca gggctaccgc gtgcgcacgg
     6781 tggagaacgt cgagcagatg gaccgcctgc tttcgcgcga gctgttcaac ctggtggtgc
     6841 tcgacctgat gctgccgggc gaggatggct tgagtgcctg ccaccgcctg cgcgagcagg
     6901 gcaacgccat gccgatcatc atgctcaccg ccaagggcga cgagtccagt cgcatccagg
     6961 gcctggagca gggcgccgac gactacctgg ccaagccgtt caatccgcgc gagctgctgg
     7021 cgcgcatccg cgccgtgctg cgccgccagg cgccgctggt gcccggcgcg ccgggcagcg
     7081 aggaggagac ggtgaccttc ggcgactacg agctgaacct cggcactcgc gagctgagcc
     7141 gcggtggaga ggtgcacatg ctcaccaccg gagagttcgc cgtgctcaag gcgctggtgc
     7201 agcacgcccg cgagccgctg actcgcgaca agctgatgag cctggcgcgc ggccgcgagt
     7261 gggacgccct ggagcgctcg atcgacgtgc agatctcgcg cctgcgccgg ctgatcgagc
     7321 cggatccgtc caagccgcgc tacatccaga cggtgtgggg cgtcggctac gtgttcgtgc
     7381 cggacggtag cgcgcgcaag tgaggacccc tctctggttt ccgcagagcg tgttcgcccg
     7441 catcctctgg ctggtgctga tcgtcgtgct gttctccaag gcgctgaccc tggtctacct
     7501 gctgatgaac caggacatgc tggtcgaccg ccagtacagc cacggcaccg cgctgaccgt
     7561 gcgtgcctac tgggcggccg acgaatcgtc gcgcgcggcc atcgccgagg cggccggcct
     7621 gcgccagctc aggcaggacg aggtgccgca gggcgagtac cactggccct acaccgaggt
     7681 gctcgaggaa cagctgcgcc tggaactggg cgaggcgacc gaactgcgca tgcgcgcgca
     7741 gcgctcgccc aagctgtggg tgctggcacc ggcgctcggt ccggactggg tcgaggtgcc
     7801 gctgtatccc tatccgctca agggccagcg catctggagc gtgctcggct ggttcctcgc
     7861 catcggcctg ctgtccaccg ccgcggcctg gctactggtg cgccagctca gcctgccgct
     7921 caagcgcctg gtgttcgccg cccggcagat cggccgcgga cgcagcgtgc gcctgccggt
     7981 gggcgatacg ccgagcgaga tgaccgagct gtaccgcgcc ttcaaccaga tgtccgccga
     8041 cgtcgagcag gccgcccgcg agcgcgagct gatgctcgcc ggggtgtccc acgacctgcg
     8101 cacgccgctg acccgcctgc gcctgtcgct ggagctgctg cccgccgacg acgagttgac
     8161 cgaggacatg gtgcgcgaca tcgaggacat ggacgcgatc ctcgaccagt tcctcgcctt
     8221 catccgcgac ggccgcgacg agccgctgga ggagctggac ctcaacgagc tgatccgcga
     8281 ggtggcggcg ccgctcaacc agagcgagga gcgcgtgcgc ctgtgcctgg agccgctgcc
     8341 ggacatgccg ctgcgccggg tgtcgatcaa gcgcctgctg gccaacctga tgggcaatgc
     8401 cctgcgccat ggcggcagtg gcgtggaagt gtgctccagc ctgtccggcg gggcggcggc
     8461 accctacgtg gtgctcagtg tgctggatcg cggcccgggc atcgatccgg ccgagctgga
     8521 gaccatcttc aatccgttca tccgcggcga ccgcgcgcgc agcacccagg gcactggcct
     8581 cggcctggcc atcgtcaagc gcatcgccgc ccagcacggc ggcagcgtcg agctgcgcaa
     8641 ccgctccggc ggcggggtgg aggcacgggt gtgtctgccg ctgggcttgc tgctgccgag
     8701 ggatgcggcg taggggtagg tcgggttagg ccgtaggccg taacccgaca accggccggc
     8761 aggccggcca ggggacacct gcggcatcct tggcgggtta cgccgctgcg cggctaaccc
     8821 gccctacgag ggggatcggc gatgacatcc ctgtcggggc tccgcgtagg gccgagcccc
     8881 cggctcaccc ccgccccttg gtgcgcgtct ggttgggccg ggcgtgctcc tcgatgtgct
     8941 ggatgatcat cgccgccacg tcctgaccgg tggtggtctc gatgccctcc aggcccggcg
     9001 aggagttgac ctccatcacc agcgggccgt ggttggagcg caggatgtcg accccggcga
     9061 cgcccaggcc catgaccttg gcggcgcgga tcgcggtcat gcgctcctcg ggggtgatct
     9121 tgatcaggct ggccgagccg ccgcggtgca ggttggagcg gaactcgccg ggcttggcct
     9181 ggcgcttcat cgccgcgatc accttgttgc cgaccacgaa gcagcggatg tcggcgccgc
     9241 cggcctcctt gatgtactcc tggaccatga tgttctgctt gaggcccatg aacgcctcga
     9301 tcaccgactc ggcggccttc tccgtctcgc acagcaccac gccgatgccc tgggtgcctt
     9361 ccagcacctt gattaccagc ggcgcgccac tgaccatgcg gatcaggtcg ggaatgtcgt
     9421 ccggcgagtg ggcgaagccg gtcaccggta ggccgatgcc cttgcgcgac agcagctgca
     9481 gcgagcgcag cttgtcgcgc gagcggctga tggccaccga ctcgttgagc gggaacacgc
     9541 ccatcatctc gaactggcgc agcaccgcgc agccatagaa ggtcaccgag gcgccgatgc
     9601 gcgggatcac cgcgtcgaag ccctccagcg actggccgcg gtagtggatc tgcggcttgt
     9661 ggctggcgat gttcatgtag gcacgcaggg tgtcgatcac cagcatttca tgcccgcgct
     9721 gctggccggc ttcgaccagg cggcgggtgg aatacaggcg cgggttgcgc gacagtacag
     9781 cgattttcat cggacacctg aggaggtagt gggcgccgcc aacagcggct tgccctggac
     9841 ataagtgagc gcggggttga ccaccaactg gccctcgacc agcgccttgg cgccgagcag
     9901 gacgcgatag cgcatggtct tgcggcaggt cagggtgaat tctaccggcc cagaccgtgt
     9961 aaaaacgcag caagcgatgg aaatcaactg ccgggggatc ttctcagtgt ccaggaggcc
    10021 gtcggaggcc caatcaggcg caatcggtga ttctggttgt tatggcactt tcggcttcgg
    10081 cgcgtgtgct ggagggcggc aagagtcgta aaacaggcct caggccttca ttgctgccat
    10141 caaggcatcg ctgcccatca agcgcaacac gcgtttgaga ttgtaggcga gcacatgcag
    10201 gctcatctcg gtactcaccc ggtcgagcgt tctggtgagg aaatgggtgg cacccatcca
    10261 ggatttcagc gtgccgaacg ggtgctcgac cgtctgccgg cggatccgca tcatctcggg
    10321 cgcttgatcc aagcgactct gcatcgcctc gagcactgcc tcatgctccc agcgactcac
    10381 tcggcgctgt gggctcggtg tgcattgctc tttcaacgca caaccttggc agtgcgaact
    10441 ccagtatcgg tgcagcttca gccctttctc gacgcttgag taccgccaga tcagactttg
    10501 cccagccggg catcggtact cgttcgttgc cgcgtcatag atgaaatcac ccttgccgaa
    10561 gcggccagcc gctgtcgcgc ccgaggtcag cgtcttgggc acgtaaacag tgattccagc
    10621 ctcatggcac gccaggattt cttcgccttt gaaataccct ctgtctgcga ccgccgagag
    10681 ctcctcgaca cccatggcct ctcgcgcttg cttggccatc gaactcagtt ggtctcgatc
    10741 gactccgttg ttcgtgacct cgtgcgtcac gatcaggtgg tggttcgcgt cgaccgccgc
    10801 ctgcacgttg tagccgacca tgccggtgcc acgcgtcttc attgagcggg catcgggatc
    10861 ggtcagggaa atctgtttat ccggtgtttc gttgagccga acctcgattt ccttgagctc
    10921 ctgcagctta gttttcaagg tcgcgatctt gtcgtggagg cgttcggctt tgacctgcgc
    10981 cacggcaggt tcctgacgat ctgcggtatc cagtgccgtc aggtagcggt tgatgctgga
    11041 ctcgatctcc tccattcgcc gctgcagctt ggcgctggtg aagttgcggt cgcgattgtt
    11101 aaccgccttg aacttgctgc catcgatggc cacgagcgct tccgagaaca ggccaagctg
    11161 ctggcacagc accacgaact gccgacagac gccgcggatt gccttgccgt tatccttgcg
    11221 gaagttggcg atggtcttga agtccggcat caaacgtccg gtcagccaca tcaactcaac
    11281 gttgcgctga gcctcacgtt cgagacggcg gctggactgg aggcgattga gatagccgta
    11341 gatgtagatc ttcaggaggt cggcaggatg gtaggccggt ctgccagttt ccgccgggac
    11401 gacaccgtcg aaacccagcg tgccaagatc gagctcatcg acgaaaacat cgaccacccg
    11461 caccgggttg gtgtccgcca cgtagtcatc caggctctcg ggaagcagcg tgctttgccc
    11521 tcggtgctct ccctggataa agcgcttcat tggccgcccc cactgatttc gaattcatca
    11581 aatcacagca agttcgatgc caacgttttt acacagtctg ggccagtggc ggtcgccgag
    11641 caccagattg gtgcaaatta cgtagcggtg ctgggtgtgg ccggtggagc tgcggatggt
    11701 cttcatcgac accagcggcg cttcgcgccg gtagtggcgg cgttgcacca ggctgccgag
    11761 gtgggcggtg aagcgcaccc acggctggcc gtcgagctcg aagggttcga tatcgctggc
    11821 gtgcagggtc gaggtgctgg cgccggtgtc gatcttggcg cgcaggccgg ccaggcccag
    11881 ctcgggcagg gcgatccatt cgcgcaggcc gatcacggcc agatggtcga aagtcttcac
    11941 ggggctcaac tccggtctgg ctgcgcagca tggccgccgt ccgcgatggc ggggcgctgc
    12001 tgctcatctg acatctgcca gggctgccag gttcagggcg acgcggtcgc ggccgctgtg
    12061 cttggcatcg tacagcgcat cgtcggcacg ttgtaccagc gccaccacgg tatcgcccgg
    12121 catcaccgtg gccacgccgg cgctcgccgt gagcggccgt cggcagggaa agccatgctc
    12181 gcgtacccgc tgcaggatgc gttcggccag cgtggcggcc tgctcgaggg tggtctcgtg
    12241 gcagatcacc agaaactcct cgccgcccca gcggcacccg ctgtcgacgt tgcgcagtcc
    12301 gtccctgagc aggcgggcga actccaccag caccttgtcg cccatcacat ggccgaggtc
    12361 gtcattgacc gacttgaaga aatcgatgtc cagcaggatc accgacagcg gccggcagta
    12421 gcgctgggca cggaggatat ccagggcgaa tgacgaatcg atggcatggc ggttgggtag
    12481 cccggtgagc aggtccgtct ctgccattga gcgcagctgg cggttcagct tcctgagccg
    12541 ggccatccag gccaggctgg tcaccaggat ggcggcgagc acgatgccca gccagatggc
    12601 cagggtgtag tcggtgacca cgtcggtcac caccgtcagg ttcacatggc ggtcgacgat
    12661 ctgccggcgc tcgagggggg tgagggtggc gatgccccgg tcgaggatgg cgcgcagcgt
    12721 ggtttccgac ttgagcacgc ccatgcgcag ctggttgtcg tagccgggga tctgcccgga
    12781 aatcttcagg ttgaaccagc cttcgtgctt gatggtggcg gcgacgacga tcagcgagcg
    12841 cagggtcagg tcggccttgc gctccgacac cagcgccagg gcttcgcgtt ccgagtcggt
    12901 cggcacgatg cgcaggttgg ggaagtcgcg ggcgatgcgc tcggccatgg cggtgccgcg
    12961 cggcagggca atgctcttgc cttgcagcgc gccgatgtcg gaaacgaacg gatgttcctc
    13021 gcgggtgatg atcacgttcg ggtcgaccag cagcggctcg gtgaagatca gccactgctc
    13081 gcgttcgggc gtgctgttga ggaagctcag cgccaggcag tcgccgctcc gcgacgccgc
    13141 caggctttcc tcccagttgc gtctgggttg cagctggagg ttgacgccgg tgcgctgggt
    13201 caccagggcg atgaggtcgg cgctgatgcc ggcatgccgc ccggcggggt cgatgacctc
    13261 gaacggccac cagtcggggt cgacgcacag cgggaccgcc gggtggacct gcagataagc
    13321 gcgctccgag tcggtcagcg cgacggcctg ggccgctgcc gccagcggca gcccggcgag
    13381 taggaggagc gccgccagcc agtgccatcc ggccggcagg cctgcgcgct tgccggcgcg
    13441 gtgcggcggc cgtgtctgct gcaggcgctt gcgggcctcc gacatgcaca aacttcctcg
    13501 ttgttcggcc gcgacgaggt cggggcgggt cctggacgtg tcattatggg taacgcccgg
    13561 cggacctgtc atgttctgtg acggggtaga cgctggcgga gccggctggc cgtgacacga
    13621 gagagagcat gagcgaaaaa gacgaccagg cgcgcctgga caagtggctg tgggcggcgc
    13681 gcttcttcaa gactcgcgcg ctggccaagg cggcgatcga gggcggcaag gtgcactgcc
    13741 gcggcgagcg ctgcaagccg tccaaggagc ccagggtggg cgacgagctg ctgattcgca
    13801 gcggtttcga cgagcgcacg gtgatcgtcc gggtgctgtc catggtccgc cgcggcgcgc
    13861 ccgaggcgca gcagctctac gaggaaaccg ccgacagcat cgcccgtcgc gaagcggccg
    13921 cggcgctgcg caaggccggt gccctgggcg tgcagagcga cggccggccg accaagaagc
    13981 agcgccggca gatccaccag ttccgcgacg gccagtgagt cgcgccgcgc gatagattct
    14041 gcccgtgccg gcgatagacg gacgcccctt ggtcgcgccc ggcgtttgcg cctaaaatca
    14101 gcctttataa agaaaacccg cgggtctggc atgtccgatt tttcccaacg cttcctgttc
    14161 gaagacaccg atgtgcgcgg cgaactggtc gaactcgatc aaagctacgc cgaggtgctg
    14221 gccaaacacg cctatcccga gccggtggcc cagctgcttg gcgagatgct cgcggcggtc
    14281 actctgctca gcgacatcct caagtacgac ggcctgctgg tgctgcaggc gcgttccgcc
    14341 ggcccggtgc cgctgctgat ggtcgagtgc tccagcgagc gcgagatccg cggcatcgcc
    14401 cgctacgacg ctgaggcgct gggcacggcg agcagcctgc gcgagctgat gcccgagggc
    14461 gtgctggcga tgaccgtcga tcccaaggtc ggccagcgct accagggcat cgtcagtctg
    14521 gacagcgaga cgctgtccga ctgcctgtcc gactatttcg ccctgtccca gcagctgccc
    14581 tcgcgcttct ggctgaatgc cgacggtcgc cgcgcccgcg gcctgctgct gcagcagttg
    14641 ccggccgacc gcctgaccga tggcgaggag cgtgcggcca gctgggagca cctggtgacc
    14701 ctggccgaca ccctgaccgc cgaggaactg ctcggtctgg acaatgagac gctgctgtat
    14761 cgcctgtatc atcaggaagt ggtgcgcgtg ttcgacgccg agccgctgcg cttcggctgc
    14821 agctgctcgc gcgagcgttc ggccaacgcc ctggtcagcc taggcgaggc cgatgccctg
    14881 cagctggtcg aggagcaggg tggggcggtg catatcgatt gccagttctg caaccaacgc
    14941 tacccgttcg atgcggccga catcgcccag ctgtttgccg gcggcggcag tgacgcgccg
    15001 tcgcccacca agcactgagt gggcattgag tgttttttac ccagaacgca acttttctgg
    15061 cataatcgcg cgcaattttt ctcgctgtag cgcgcctata agaaccgcta caaaacgatt
    15121 ggaggactcg gcctcaggcc gacggggacc acatgacgca agccaatact gccgtgtaca
    15181 ccgatatcag cgttgccgaa ctgatcgagc aggccatcca gcgtggcgag ggtgagctcg
    15241 ctgccaatgg ttcgctggtc gtgcgcacgg gtcaccgtac cggtcgttcg ccgatggacc
    15301 gctacatcgt cgaggaatcg agcacccagg ccaagatcgc ctggggcaag atcaaccgtc
    15361 cgttcccgga agacaagttc gctgccctgt gggatcgcgt cgaagcctat ctgggcgagc
    15421 gcagccactt cgtctcccac gtgcatgtcg gctccgccga agagcactac ctgccggtga
    15481 agatgagcac cgagaccgcc tggcacaacc tgttcggccg ctgcctgttc atcaacccgc
    15541 aaaccttcaa cccggctggc aagggcgagt ggcgcatcct caacgtgccg accttcgtct
    15601 gcgagccgga gcgtgatggc accaactccg acggttgcgt gatcctcaac ttcgccgaga
    15661 agaaggtgct gatcgccggc atgcgctacg ccggcgagat gaagaaggcc atgttctccg
    15721 tgcagaactt cctgctgccg gaaaaagacg tgctgccgat gcactgcgcc gccaacatcg
    15781 gcgaagaagg cgacgtgacc ctgttcttcg gtctgtccgg caccggcaag accaccctgt
    15841 ccgccgaccc gagccgctac cttatcggcg acgacgagca cggctggggc gagggcgtgg
    15901 tgttcaacat cgagggcggt tgctatgcca agtgcatcga cctgtccgag aagaacgagc
    15961 cggtgatctg gagcgccatc cagttcggtg ccgtgctgga gaacgtggtg ctcgacgagc
    16021 gcacccgtca gcctgactac gccgacgcca gcctgaccca gaacagccgt gcggcctacc
    16081 cgctggaact ggtcgagaag cgctcggaga agaacctcgg cggcgagccg aacgcggtga
    16141 tcttcctgac ttgcgacctg accggcgtgc tgccgccggt gtcgatcctc aacaacgagc
    16201 aagcggccta ccacttcctg tccggctaca ccgcgctggt cggttccacc gagatgggca
    16261 gcggcagcgg catcaagtcg accttctcca cctgcttcgg cgcgccgttc ttcccgcgtc
    16321 cggccggcga gtacgccgag ctgctgatca agcgcatcaa ggcgttcggc tccaaggtct
    16381 acctggtcaa caccggctgg accggcggcg gctacggtgt cggcaagcgc ttcaacatcc
    16441 cgaccacccg tgcggtgatc gcggcgatcc agagcggcgc gctggtcggt gccgagaccg
    16501 agcacctgcc gatcatcaac ctggacgtgc cgaaggccgt cgccggcgtc gacaccgagc
    16561 tgctcaaccc gcgcaacacc tgggccgaca agagtcagta cgaccaggcc gcccgcgccc
    16621 tggctgcgct gttcgtcgac aacttcaaga agttcgaagt gtccgacgcc atccgcagtg
    16681 ccggcccgca actctaagcg gttcgctctc ggtcgttgaa aaaaccgcct tcgggcggtt
    16741 ttttcgtttc tgccggtccg gcctgccggg cgggcattcc tgccgcggag cgacgcccag
    16801 cgggtgagaa gtgggttgtg ctgcggtttg cgcagggccg tccgccctca gtcggtgccg
    16861 gcctccaggc gggcgaggcg ggcttccagc tcggcgaggc gcgcctccag ggtagcgagg
    16921 cgctcttcct gggcattcga cagcccggtt gaggcggctg gctcctcgct gcgggcggcg
    16981 agcaggttct ccagctcggc cggatcgccc aactggtggg cataacgctc ctcgcgctgt
    17041 cccggctggc gcggcagctg gcaggccagc tcgcgcacca tcaggcgttc gaggtgatgg
    17101 cgcacttcct cctggtcgtc gaaggcgtgc agccgggtgc tgcgggcgag cagctcgccg
    17161 agggtctgcg ggccgcgcag catgagcagg gcgagcagga tcagttgggc gtgcaccagc
    17221 tccagctgct tgtcgccgcg ctgctcccag cggtcggcgc ggctgcccat caccag
//
LOCUS       DATCUN010000290         6445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_88564, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000290 DATCUN010000000
VERSION     DATCUN010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88564"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VIO83_11235"
     CDS             complement(<1..809)
                     /locus_tag="VIO83_11235"
                     /inference="COORDINATES: protein motif:HMM:NF020962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HEY8331884.1"
                     /translation="MTAPSPLSAVLDAAERAGKLNLRTEEIISAMPGVGPEALRQALH
                     RQQRRGRLVRLSRGSDHWLIVPLQHAAAGAPPLETWLDRYLTKTLDTPYYVALLSAAE
                     TYGASPYAVMVTQVMVPERRRPIMVGRHEVVFHTRARIEHMPTRWHETPDGRFKVSTP
                     ELTALELVQRETLLGGMARVRAVLQALWASCTAARLSEALDALQEVPTAQRLGALLVQ
                     DGQGVLAAQVASWLRDKPLRIVSLEGGQASITSTGFDAAFKVRLPADSQSA"
     gene            1040..1654
                     /locus_tag="VIO83_11240"
     CDS             1040..1654
                     /locus_tag="VIO83_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HEY8331885.1"
                     /translation="MPPTLTHHARRGAFVASLLAAIVLTSGCTTLSVALSASVPETEP
                     DIQTLSPSRDELIPVVRYGRYTLVEMVPDAAQRDLLQQAIDVAIPPTASVMVGDALRH
                     VLLRSGYQLCEGNSDTTVLYALPLPAAHLHLGPLTLRDALLTLAGPAWDLSVDDTVRQ
                     VCFRRHVEPSSLPTATPSSTRLPDSPTVDGAAKPSVIVAPELQP"
     gene            1651..2295
                     /locus_tag="VIO83_11245"
     CDS             1651..2295
                     /locus_tag="VIO83_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331886.1"
                     /translation="MTSAISSPSRTRWLRIATATWLLLISGISLVNSVGLSRLAEQAQ
                     GSAQEGQLKAMTARVADLEQQAEASKHQPAVLSQASFTTAQQALEERLAKLEQGQAGN
                     ARADDLPPILRRLDGIETRLQRARQTAPTPAARQRTTETVQPKAVVPPFMVLGFELRG
                     GERFLSIAPTDTASLTQARLLRPGEAAGDWQLESFEGRSAVFRVGEQIRRIAVP"
     gene            2370..3047
                     /locus_tag="VIO83_11250"
     CDS             2370..3047
                     /locus_tag="VIO83_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HEY8331887.1"
                     /translation="MAQNSRAAEARVVPSQIQSSTDASLDERLARDWGLRAEEWTRYR
                     QVMQGPLGVYSPNLDPLTALGIESRTDEERRRYAELQVQAEAQRVEKTLAYQRAYDAA
                     WQRLNPGALRVNLPGAGPKSMGAASALRGSGRLAVFVKDGCVTCATIVQQLQTAGTEF
                     DLYMVGSRRDDARIRDWAQRAKVDPGRVRAHTITLNHDGGRWLSLGLPGELPAVVREV
                     NGQWQRQ"
     gene            3107..3643
                     /locus_tag="VIO83_11255"
     CDS             3107..3643
                     /locus_tag="VIO83_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY8331888.1"
                     /translation="MPSGQAQEVPPPAYQLAARQADIPSAVLYAVALQESGAKLRGRL
                     VPWPWTLNVVGTAQRYGTRVEACAGLLQALRQAPPTRVDVGLGQINVGYQDHRFDHPC
                     ELLDPYRNLAIAAAILREQHTTDGEDWLLAIGRYHRPAGGVPAVRYRRSVSRHLSRVL
                     GPTPGAVLPRSSNSTPRP"
     gene            3694..4179
                     /locus_tag="VIO83_11260"
     CDS             3694..4179
                     /locus_tag="VIO83_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HEY8331889.1"
                     /translation="MLPALAASPPLVVVEDRGGTSALPYYQALNPQAHEDAAAPTLPA
                     APPPARPVTEAVMLPVRSPALSPGDVPQRVIRAPGLRPLFLVGDDPRSRAWLRARLPI
                     LRDLGAMGLVVQVETPAALAELRALAPGLTLSPMSGDDLAARLGLRHYPVLVTATGVE
                     Q"
     gene            4190..6364
                     /gene="traD"
                     /locus_tag="VIO83_11265"
     CDS             4190..6364
                     /gene="traD"
                     /locus_tag="VIO83_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HEY8331890.1"
                     /translation="MAQPHAVEVLLRPAVELYTVAVCAGAAVLCLVAPWSLALNPLLG
                     LGSALTFLAFGAIRLAEAGAILRYRRNIRRLPRYVMTSREVPVSQQRLFVGRGFRWDQ
                     RHTHRLMQTYRPEFRRYVEPTPLYRIARRVEERLEFAPYPFSHLARLTAWDSPLNPVR
                     PLPPLGGLPRLHGIEPHEVDVALPLAERVGHTLVLGTTRVGKTRLAELFITQDIRRRN
                     SAGEHEVVIVFDPKGDADLLKRVYIEARRAGREGEFYVFHLGWPDISARYNAVGRFGR
                     ISEVATRIAGQLSGEGNSAAFREFAWRFVNIIARALVELGQRPDYLLIQRHVINIDAL
                     FIEYAAQFFAKTEPKAWEVIVQLEGRLNDKNIPRNMIGREKRVVAIEQYLSQVRVYDP
                     VLDGLRSAVRYDRTYFDKIVASLLPLLEKLTTGKIAQLLAPNYSDLNDPRPIFDWMQI
                     VRKRAVVYVGLDALSDAEVAAAVGNSMFADLVSVAGHIYKFGIDDGLPDALAGARVPI
                     NVHADEFNELMGDEFVPLVNKGGGAGLQVTAYTQTLSDIEARVGNRAKAGQVIGNFNN
                     LFMLRVRETATAELLTRQLPKVDVYTTTLVSGATDSSDVRGPTDFTSNAQDRISTASV
                     PMIEPAHVVGLPKGQAFALLQGGQLWKIRMPLPAPDPDEALPEDLQQLAGYMRHHYAE
                     AGDWWENQGLPGLQDQPLPDDLLDGFRHLADPDTAVDSEARP"
BASE COUNT         1044 a         2316 c         2058 g         1027 t
ORIGIN      
        1 ttggcactct gtgagtcggc gggaagccgc accttgaagg cggcgtcgaa accggtggat
       61 gtgatggatg cctgcccacc ttcgagcgac acgatgcgca gcggcttgtc gcgcagccag
      121 cttgcgacct gggccgcaag cacgccctgg ccgtcctgga ccagcaaagc gcccaggcgc
      181 tgggcggtag gcacttcctg cagcgcgtcg agcgcctccg acagcctcgc tgccgtgcag
      241 gacgcccaga gcgcctgcag taccgcccgc acccgtgcca tgccgccgag cagcgtttcg
      301 cgctgcacca gttccagcgc cgtgagctcc ggcgtgctga ccttgaagcg tccatccggc
      361 gtttcgtgcc agcgggtcgg catgtgctcg atccgtgcgc gcgtatggaa caccacttca
      421 tgccggccga ccatgatcgg ccggcgacgt tcgggcacca tcacctgggt caccatcact
      481 gcatagggtg aagcgccata ggtctctgcc gcgctcaaca gcgcgacgta atacggcgtg
      541 tccagggtct tggtcagata acggtcgagc caggtttcca gcggaggcgc accggcagcg
      601 gcatgctgca gcggcacgat cagccaatgg tccgagccgc gcgacagccg gaccagccgc
      661 ccgcgccgtt gctgccgatg cagggcctgg cgcagcgctt cggggccgac acccggcatg
      721 gccgagatga tttcctcagt gcgcaggttc agcttgccag cgcgctcggc cgcgtcgaga
      781 acggcggaca ggggactggg agcggtcatg ggctcgactc attcatttcg cgcaatgtat
      841 cactaaacgt cacgtagcgc gaagtcaatg ttaattgtgg cgcaggtgat ccacgcgccc
      901 gatgcgtgtc ctcgacacga tagccgcctc cacggtccgc ccgccagata aatcggtcga
      961 gctgcggttc cctttgtctg ctgatgcgac ggcctttccc tgtgatggtg gcgtcaccac
     1021 gacaccggga aatcaaggca tgccaccgac tttgactcac cacgccagac gcggcgcatt
     1081 cgtcgccagc ctgctggcgg ccatcgtgct gacgagtgga tgcaccacat tatccgtggc
     1141 gctttccgcc tcagttccgg agacggagcc ggacatccag accctatcgc catctcgcga
     1201 cgaactgatc ccggtcgtcc gctatggccg ctacacgctg gtggaaatgg tgcccgacgc
     1261 cgcacaacgg gacttgctgc aacaggccat cgatgtcgcc atccccccga cggcaagcgt
     1321 gatggtgggc gatgcgctac gccatgtact gctgcgttcg ggctatcagc tctgcgaagg
     1381 caacagcgac acgaccgtgc tctacgcgct gccgctgccg gcagcgcatc tgcacctggg
     1441 gcctctgacg ctacgcgatg ccctcctgac cctcgccggg ccggcctggg atctgtcggt
     1501 cgacgacacg gtccgccaag tctgcttccg ccgacacgtc gagccctcat cgctccccac
     1561 tgcgacgcca tcgagtaccc gtctccccga ttccccaact gtcgatggcg ccgcgaagcc
     1621 ctccgtcatc gtcgcaccgg agttgcagcc atgacgtcgg cgatttcatc tccctcgcgc
     1681 acgcgctggc tgcgcattgc cacggccacc tggctgctgc tgatcagcgg catctccctc
     1741 gtcaacagcg tcggactgtc ccgtctggcc gagcaggcac agggcagcgc ccaggagggc
     1801 caactcaaag ccatgacggc gcgcgtggcc gaccttgaac agcaggccga agccagcaag
     1861 caccagcccg cggtactcag ccaagcaagt ttcacgaccg cgcagcaagc actcgaagaa
     1921 cggctggcca agctcgaaca agggcaggct ggcaatgccc gcgccgacga cctgccgccg
     1981 atcctgaggc gcctggacgg tatcgagacg cggctgcaac gcgcccgcca aaccgccccc
     2041 acaccggcag cacgccagcg caccaccgag accgtgcaac ccaaggcggt tgtcccgccc
     2101 ttcatggtgc tgggcttcga gttgcgcggc ggcgaacgct tcctgtcgat cgcaccgacc
     2161 gacacggcat cgctgaccca ggcccggctg ctgcgtcccg gcgaagccgc gggcgactgg
     2221 cagctcgaat cctttgaagg acgcagtgcc gtattccgcg tcggcgaaca gatccggcgc
     2281 atcgccgtgc cgtaggaggc gccatgaacc cgccgtcccg ccttgccctc ctggcgctcg
     2341 ccatcgccgc cagcctgctt cccgccgcga tggcacagaa cagccgcgcc gccgaggcgc
     2401 gcgtcgtccc ttcgcagatc cagtccagca ccgatgccag tctggacgaa cggctggcac
     2461 gcgactgggg cctgcgcgcc gaggagtgga cgcgttaccg gcaagtcatg cagggcccgc
     2521 tgggtgtgta ctcgcccaac ctcgatccgc tcaccgcgct gggcatcgaa tcgcgcacgg
     2581 acgaggaacg ccgccgctat gccgaactgc aggtgcaagc cgaagcacag cgcgtcgaga
     2641 agaccctggc ctatcagcgc gcctacgacg ccgcgtggca gcgcctgaac cccggcgccc
     2701 tgcgcgtgaa cctgcctggc gctggcccca agagcatggg cgcggcatcg gccctccggg
     2761 gttcgggccg cctggcggtc ttcgtcaagg atgggtgcgt gacttgcgcc accatcgttc
     2821 agcagttgca gaccgccggc accgagttcg acctctacat ggtcggtagc cgccgtgacg
     2881 acgcgcgcat ccgcgactgg gcgcagcgcg ccaaggtcga tccgggccgc gtgcgggcgc
     2941 acacgatcac cctcaaccat gatggcggcc gctggctgtc cctgggcctg cccggcgaac
     3001 tgcccgccgt ggtgcgcgag gtcaacggcc aatggcaacg gcagtaattc gctggctcgg
     3061 gcgacggctt gccgggctcg gcacggtcct gtgtctgtcc gccgcgatgc cgtccggcca
     3121 ggcccaggaa gtgccgccac cggcctatca acttgccgcg cggcaggcgg acatcccctc
     3181 ggcagtcctg tacgcggtgg ccttgcaaga gagtggcgcc aagctgcgtg gccgcctggt
     3241 tccatggccc tggacgctca acgtggtcgg caccgcacag cgttacggca cccgcgtcga
     3301 agcctgcgcc ggcctgctgc aggcgctgcg gcaagccccg ccgacccgtg tcgatgtggg
     3361 cctgggccag atcaatgtcg gctaccagga tcaccgcttc gaccatccct gcgaactgct
     3421 cgacccgtac cgcaacctgg cgatcgctgc ggcgatcctg cgcgagcagc acaccaccga
     3481 tggcgaagac tggctgctcg ccatcggccg ctaccaccgc cccgccggcg gggtgcccgc
     3541 cgttcgctac cggcgcagcg tcagccgaca cctgtcgcgc gtgctagggc cgacccctgg
     3601 cgccgtgctc ccccgctcat ccaactcgac accccggcca tgacccgacg tatccccctt
     3661 cgtgcgcttt acagcctgtt cctgatcgct caaatgctcc cggccctggc cgcttcaccc
     3721 ccgctggtcg tggtcgagga tcgcggcggc acgtcggcac tgccgtacta ccaggccttg
     3781 aacccgcaag cgcacgaaga cgccgcggca cccaccctgc ccgctgcgcc accgcccgcg
     3841 agacccgtca ccgaggcagt catgctgccg gtgcgctcgc ccgcactgtc gccgggcgat
     3901 gtgccgcagc gggtcatccg ggcaccggga ttgaggccgc tgttcctggt cggcgacgat
     3961 ccgcgatcgc gcgcctggct cagggcgcgc ctgccgatcc tgcgtgatct cggggcaatg
     4021 ggcctggtcg tgcaggtcga gacgcctgcc gcactggccg aactgcgcgc gttggcgcca
     4081 ggcctgacct tatcgccgat gtccggcgac gacctggctg cgcggctggg actgcggcac
     4141 tacccggtgc tggtcacggc caccggcgtc gagcagtgag gtccgtggga tggcgcagcc
     4201 gcatgcggtc gaggtgctgt tgcggccagc ggtggagcta tacaccgtgg cagtctgcgc
     4261 cggcgccgcg gttctgtgcc tggtggcccc gtggtcgctt gcgctgaatc cgctgctggg
     4321 actgggctcg gccttgacct tcctggcctt cggcgccatc cgcctcgccg aggcgggggc
     4381 catcctgcgc taccgccgca acatccgccg cctgccgcgt tacgtgatga cgagccgcga
     4441 ggtgccggta agccagcagc ggttgttcgt cgggcggggc tttcgctggg accagcgcca
     4501 cacgcaccgg ttgatgcaga cctaccggcc cgagttccgg cgctacgtcg agccgacgcc
     4561 cctctaccgg atcgcacggc gcgtggagga gcgattggag ttcgcgccgt atccgttctc
     4621 gcacctggcg aggctgactg cctgggatag tccgctcaat cccgtgcgcc ccctgccacc
     4681 cttgggcggt ctaccgcgat tgcacggcat cgagccgcac gaggtcgacg tcgccttgcc
     4741 gctcgccgag cgcgtcggcc acaccctggt gctgggcacc acgcgcgtgg gcaagacacg
     4801 cttggccgaa ctcttcatca cccaggacat ccgccgcagg aacagtgccg gcgagcacga
     4861 ggtggtgatc gtcttcgacc ccaagggcga cgcggacctg ttgaagcgcg tatacatcga
     4921 ggccaggcgc gccggacgcg agggcgagtt ctacgtcttc cacctgggtt ggccggacat
     4981 ctcggcgcgc tacaacgccg tgggacgctt cggccgtatc tcggaggtgg ccacgcgcat
     5041 tgccgggcag ttgtccggcg aaggcaactc ggcggcgttc cgcgagttcg cctggcgctt
     5101 cgtcaacatc atcgcgcgcg ccctggtcga gctgggtcag cgcccggact acctcttgat
     5161 ccagcgccac gtcatcaaca tcgatgcgct gttcatcgaa tacgcagcgc agttcttcgc
     5221 caagacagaa cccaaggcct gggaggtgat cgtccagctc gaaggccggc tcaacgacaa
     5281 gaacatcccg cgcaacatga tcggccgcga gaagcgcgtc gtcgccatcg agcaatatct
     5341 gtcgcaggtg cgggtctacg acccggtgct cgatgggctg cgcagcgcgg tgcgctacga
     5401 ccgcacctac ttcgacaaga tcgtggcctc gctgctgccg ctgctggaga agctcaccac
     5461 cggcaagatc gcgcagctct tggcgcccaa ctactcggac ctcaacgatc ccaggccgat
     5521 cttcgactgg atgcagatcg tgcgcaaacg cgcggtggtc tatgtcggcc tcgatgccct
     5581 gtcggatgcc gaggtcgccg ccgccgtcgg caactcgatg tttgccgatc tggtgtcggt
     5641 cgccgggcat atctacaagt tcggcatcga cgatgggctg cctgacgctc tggcgggtgc
     5701 ccgggtgccg atcaacgtcc atgcggacga gttcaacgag ctgatgggcg acgagttcgt
     5761 gccgctggtc aacaaaggcg ggggggccgg gctgcaggtc acggcctaca ctcagacgct
     5821 cagcgacatc gaggcgcgtg tcggcaaccg cgccaaagcc ggccaggtca ttggcaactt
     5881 caacaacctg tttatgctga gggtgcgcga gaccgcgacc gccgagctgc tcacccgcca
     5941 gctgcccaaa gtggacgtct acaccacgac gctggtcagc ggcgccaccg acagctccga
     6001 tgtgcgaggg ccgaccgact tcacctccaa cgcacaggac cgcatcagca ctgccagcgt
     6061 gccgatgatc gagcccgcac atgtggtcgg cctgcccaaa ggccaggcct ttgccctgct
     6121 gcaaggcggc cagctgtgga aaattcgcat gccgctgccg gcccccgatc cggatgaagc
     6181 cctgcccgag gatctccagc agctcgccgg ctacatgcgt catcactatg ccgaggccgg
     6241 cgactggtgg gaaaaccagg gcctgccagg cctgcaggat caaccgctgc ccgatgacct
     6301 gctcgacggc ttccgccacc tggccgatcc ggacactgcc gtcgacagcg aggcgcggcc
     6361 atgaaggacc cggccgccac cacccagcgc gagcagactc gccaacaagg cttgctcgct
     6421 ggcatcgtca ccctgccact acgca
//
LOCUS       DATCUN010000291        18073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_126926, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000291 DATCUN010000000
VERSION     DATCUN010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 18073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18073
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126926"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /gene="madL"
                     /locus_tag="VIO83_11270"
     CDS             <1..411
                     /gene="madL"
                     /locus_tag="VIO83_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012314726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005342 - organic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015849 - organic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate transporter subunit MadL"
                     /protein_id="HEY8331891.1"
                     /translation="CTLAGLFVGDLLGVLLGVPSNVGGVGIAMILLIFGGSYLSKRGL
                     LAGKTEQGIEFWSAIYIPIVVAMAAQQNVLGALSSGPVAILAGLAAVSISFALVPVLD
                     RLGRKDEVAPEPDAKPVTPATAPVVTPATSSAQR"
     gene            420..1181
                     /gene="madM"
                     /locus_tag="VIO83_11275"
     CDS             420..1181
                     /gene="madM"
                     /locus_tag="VIO83_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005342 - organic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015849 - organic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate transporter subunit MadM"
                     /protein_id="HEY8331892.1"
                     /translation="MLESLTKVLNGYGMISGFAVIGATMWLSYWASAKLTKGRLHGSA
                     IAILVGLVLSYVGGLYTGGQKGLVDIPLLSGIGILGGAMLRDFAIIATGFGVNPAELK
                     RAGASGVLALIVGIFASFVAGAGVALAFGYTDAVSLTTIGTGAVTYIVGPVTGAAIGA
                     SSEVMALSIAAGLIKAIVVMVATPFVAPFIGLNNPRAAVIFGGLMGTSSGVAGGLAAT
                     DPKLVPYGCLTAAFYTALGCLLGPSLIYFVMRAVF"
     gene            1380..1559
                     /locus_tag="VIO83_11280"
     CDS             1380..1559
                     /locus_tag="VIO83_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEY8331893.1"
                     /translation="MKSARTTISLPPEQLEQLQALADRHGLSLAWVIRQAIGEFLARV
                     EHPANFDPLASRKES"
     gene            1559..4387
                     /locus_tag="VIO83_11285"
     CDS             1559..4387
                     /locus_tag="VIO83_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEY8331894.1"
                     /translation="MYTPYQRAYLAHWLSLSRGEEQALTQTIASAKVDMNPHQVDAAL
                     FALRSPLSQGVLLADEVGLGKTIEASLVLAQKWAEQRRKLLLIVPATLRKQWSQELED
                     KFALPAVILEAKNFNEACKRGVGNPLDIEQAFGRPAIAICSYEFAARKQRELGLIPWD
                     LVVFDEAHKMRNIYKGEGAKTAKALDEALKGRTKVLLSATPMQNNLLELYGLVSIVDP
                     HFFGDLESFKARYSKPRLSQAELDQLRQRLKSICSRTLRRQVQEEGGINFTRRYSMTE
                     PYDPQPDEQELYRKVSSYLQDESVLAIKPNARHLVTLVIRKILASSSHAIQGTLQRMV
                     ERLEKRMSLADALSDYEELDDLADTSGVDEDDFVDPAVLQAEIERLKEFLNLAASIER
                     NAKGDALLTVLGRSFDFAERLGGQRKAVIFTESVRTQAWLFELLSSEGYRDKVVLLNG
                     SNSDPESLRTYQAWLEKHQGSARVSGSKSADMKAALVERFREQASIMICTEAGAEGIN
                     LQFCSLLINYDLPWNPQRVEQRIGRVHRYGQKHDVVVVNFLNNSNRADQRVYQLLSQK
                     FQLFEGVFGSSDEILGAIESGVDIEQRIHAIYQKCRSDEEIEEQFNQLQEQLQAQIET
                     RNQDARRSLLEHFDVDVVARLNMCRERTQQALDDYQQRLLLLARMSVPQARFEGERFE
                     LDGHWYDPNWQRAQETDARFFQPNDGKGGELIERAKATLSAPAAAELLLRYVPAENGQ
                     LSDVLELLGQSGELVVEKLTLSTATQRREHLLLAGFTDSGQLLDERTCDRLLRLNAQE
                     VSTQPALQHVATLQARLDALRGECLQQANAHNERYFEEETEKLERWAEDKRIGLDIRI
                     KQLDQEIKEARKAVRQLETLQEKMSAKKALKKLERERDNLMLDYHEEKKHIEAEEDRL
                     LEAIEQNLEISVKHERLFSLRWRLEGTA"
     gene            4384..5661
                     /locus_tag="VIO83_11290"
     CDS             4384..5661
                     /locus_tag="VIO83_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8331895.1"
                     /translation="MINTTELVRELIYQIRLGEDSAYEFKAVTLHDNKVTKPHRDSVA
                     DELAAFANGSGGFLILGVNDKTREVEGIALEQLDTVELWLTNMASQAIDPPLPIETRL
                     VEIPDHQGNARPVVWVRVAKSLFVHRSPHGYFHRVGSSKREMSPELLARLFQQRSMAR
                     LIRFDEQLVPNASRDDIDPELKARFLRSDLPEALQLKKLYLLLEDENRQPHLTVTGVL
                     LLTRRPADFLSSAYVQCVAYSGSERSAEYQLDAQDCDGPVDQQIQQAFAFVKRNMRVQ
                     AVKRPGRIDIPQYDLGAVYEALVNAVAHRDYAMHGARIRVHLFADRLEISTPGGLPNS
                     LTVESMDANSISRNETLVNLLSRYYPADPATRRQNMIERRGEGVPTIIAASERLSLRR
                     PLYEQIDHTELKLTIYAASRERNGLVAQFADDE"
     gene            5673..6251
                     /locus_tag="VIO83_11295"
     CDS             5673..6251
                     /locus_tag="VIO83_11295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY8331896.1"
                     /translation="MSEKQKLELTWIGKDKRPRLEPRILIEDKALSYRAEAIAPLQGD
                     LLAGEQPPSYNDNLLIQGDNLLALKALESQYAGKVKCIFIDPPYNTGSAFTHYDDGLE
                     HSLWLTMMRDRLEIIRTLLADDGSLWITIDDNEAHYLKVLCDEVFGRRNFVANIVWQK
                     RTSRENRAAVGSAHDHLLIYAKCGAVDWKSTR"
     gene            6354..7301
                     /locus_tag="VIO83_11300"
     CDS             6354..7301
                     /locus_tag="VIO83_11300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY8331897.1"
                     /translation="MYRIKTSSGDFVDPPKGRCWGATEPVYLEYLRDNRVYFPNGGTG
                     KPRIKQFRGEEKGLVPMTWWDAEFAGDNEAAKKEILALFPDDEPFGTPKPEQLLERVI
                     HIATRPGDLVLDSFAGSGTTGAVAHKMGRRWIMVELGEHCHTHIVPRLKKVIDGADQG
                     GISKAVDWQGGGGFRYLRLAPSLLKKDQWGNWVINPAYNAEMLAEAMCKHMGFSYAPS
                     QTHFWMHGRSSETDFIYVTTGSLSHDQLRLISEEVGSQRSLLICCKAFMADTEAFPNL
                     TLKKIPKAILGKCEWDHDDYSFSINLTAEDASEEPLEDE"
     gene            7319..10102
                     /locus_tag="VIO83_11305"
     CDS             7319..10102
                     /locus_tag="VIO83_11305"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEY8331898.1"
                     /translation="MAEPINGQKVANQVTARLSLRKPQRESLQVLVDLLDQLPLGKEP
                     DLQHLLQVIQAHYPSMQAFERDFPSLCFALATGVGKTRLMGAMIAWLFITGRSRHFLV
                     LAPNLTIYEKLKKDFTLGQPKYVFAGIPEMANTPPVVITGEDYEDGRGVRLDYAVPAM
                     LSGDLFGADASLHINIFNIAKINARDNKKGAVKSATAKVRRLQEYLGESYFDYLAALP
                     DLVVLMDEAHRYYADAGAKAINDLKPVLGLELTATPKTVGAKPRPFGNVVYHYPLSSA
                     LKDGYVKIPAVATRKDFRAEQYSQDALETIKLEDGIHHHEYVKVELENYARSQGVSAV
                     KPFMLVVAQDTTHAAAIRARIESEDFFSGRYRGRVIEVHSNQSGEESDESMQRLLAVE
                     HDEATEVVIHVNKLKEGWDVTNLYTIVPLRASASEILTEQTIGRGLRLPYGKRTGVEA
                     VDRLCIIAHDRFQEIIDRANKDDSLIHEKIYIGSGADADVPDRQPQQVEASPVSVVQT
                     SREDATPTAENAGRYQVAVRSEQEAKIAAAAIQAVQEEARKLSSSLQLGDEDVQQKLV
                     RRVQRALQEQLPVQQQLAGLESEALPSAAEIESLVRSVTEKAVELTIDVPHITVLPTR
                     EVNYGFQNFDLQNLASIKLEPVSQEMLLRHLENDHSASIQWGRQEAREPRLEDYLVRH
                     LFTRDEVDYDQYAGLLYKLAGQVIAHLQSYLKDSDEVENVLLYWQKQLSDFIWAQMQA
                     QRWTTPCDYRGKVIAGFDVLRPATFTLPAGDKPRHFRTPVEDKRAIRQMVFEGFKRCC
                     FPYQKFDSVDGEWRLAQVLEDDDKVLKWMKPAPGQFRIEYLDGRNYEPDFVVETESGY
                     LIIEPKRADQIGHEEVRAKARAAMRWCGFANEHAQSNGGKPWTYLLVPDDQIVLGVSV
                     ARLVTEFATNLPG"
     gene            complement(10131..11048)
                     /locus_tag="VIO83_11310"
     CDS             complement(10131..11048)
                     /locus_tag="VIO83_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8331899.1"
                     /translation="MQIDEELTFKKLEIFLAFMRSGNLSRAAAELDTSTVSVHRAIHS
                     LENALRCPLFKREGRKLTPLESAHVLEDRAQKLIADMLDTVRLTREAAGFSAERFKLG
                     ALYSLTVKTVPQLIMGLKLRRSELNIDLILGSNVDLLYKLKNLEVDAILVSLNDSVSD
                     PDCESLPLFCDDIFLAVPTDSPFAGQAEVDLAELREATFITLSQGFATHQDAQRVFRQ
                     AGFAPKVAMQVNDIFTLLSMVSSGVGYALLPGRIAAVYENRVRLIPLQARYRMQQQIG
                     LVFLKAKERDPNLLALLAECRMYAKRHEG"
     gene            11278..12201
                     /locus_tag="VIO83_11315"
     CDS             11278..12201
                     /locus_tag="VIO83_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8331900.1"
                     /translation="MIEIEKLTKRFVQHTVVDDLSFRVQPGEVLGFLGPNGAGKSTTM
                     KMLTGFLAPNSGSARLFGFDIQKDTLKAQRLIGYLPEGAPCYGDMQVRGFLEFIAEVR
                     GYRGAEKRSRVARAVEQVELQAVLGQSIDTLSKGFKRRVGLAQAILHDPRALILDEPT
                     DGLDPNQKHQVRQLIQNLATDKIIIISTHILEEVTAVCSRAVVIAAGRLVADGTPFEL
                     ESRSRYHQAVTLVGDAPLDEVALAALPGVAGVERNAREHSLTVLAQPGQVIFPHVSAL
                     VAARGWSIKEMDVERGRLDEVFRTLTRGEAA"
     gene            12198..12932
                     /locus_tag="VIO83_11320"
     CDS             12198..12932
                     /locus_tag="VIO83_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8331901.1"
                     /translation="MRQLPVVFKRELASYFATPLAYVFIVIFLALSGVFTFYLGSFFE
                     RGQADLGAFFSFHPWLYLFLVPALAMRLWAEERKSGTIELLMTLPITRADAVGGKFLA
                     AWAFAGIALLLTFPMVLTVNYLGEPDNGAILTGYIGSWLLAGGYLAIGSCLSALAKNQ
                     VIAFILTVCVCFLFVASGFPLVLDAFSGWAPQALVDAVASLSFLTRFDAISKGVLDLR
                     DLLYFASLILAWLTATAVVLDLKKAD"
     gene            12942..14774
                     /locus_tag="VIO83_11325"
     CDS             12942..14774
                     /locus_tag="VIO83_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEY8331902.1"
                     /translation="MKRIIYSGTGLLLIALVFLAFNMVTGLGLHNARLDLTEQKLYTI
                     SEGTERILGQLDEPINLYFFYSDNVSQDLVPLRNYAKRVEEMLHAYERAAGGKLHLKV
                     IDPEPFSEDEDRAAEFGLQGVPLQQGGDKLYFGLAGTNALDNVQSIPFFPLDQEEFLE
                     YEISRLIQSLAKPERPVVGVLSGLQMDGSFNPLTGQAGTPWMVMEEIRQQFSIKSLRN
                     DSDRIPDEVSVLLLVHPKDLPPPTLYAIDQFVLRGGKLLAFVDPFSESDNSGMLGNGQ
                     GNASDLEPLFKAWGVRLRAGQVLADGTYAMAVGVGVGEEQRPVRHPAWLGLPKAALDQ
                     TDVTTAALENITVASAGILEPVEGAKTRFTPLLQSSEYAMPFDAQRLAGLRDPQELMR
                     ELEPTGERYAVAARVSGPAPSAYPEGIEGQKDGLKSAESINVIVVADTDLLSDRMWVQ
                     VQEFFGQRVPQPFADNAGFAINALDNLAGSDALISVRSRGRFSRPFVVVERLQREAEA
                     RFREQEEVLKQQLAQTEQRLAELQQSDSPEQVLELTPEQQATLQQFMQDKLKIRKELR
                     EVRYQLNADIEGLGRVLKFVNIALVPLLLTLGVIVLWLFRRRRA"
     gene            14785..15762
                     /locus_tag="VIO83_11330"
     CDS             14785..15762
                     /locus_tag="VIO83_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEY8331903.1"
                     /translation="MRRSALLVLLLIALGLGGLFAWLQRSAEVPQQAARQLLLPVLAG
                     HLEQVRALEIRHGDLPAIRIERAGGGWIVPAKAGYPAAAGEVNRLLRALAEAHRVEAK
                     TRNPDNHARLDLAERGEGAATRLTLEGVGKTPVVVLIGQAARQGGQLVRLAGDDQVWS
                     IDQSLQLVDNELAWLDRRISAIPFASVRELEVRHADGEQLNVWRATPEQANLALRQLP
                     KGRTLAYEPVANGMAALFSALDFADAAPLAQVGFKARPELEFSLQTLDGGELHGAFHQ
                     QGGQHWLVLGDSKGLDGRLIAGKDWAYRLEEQQYRTLAKRVKDLLGPQG"
     gene            16007..16216
                     /locus_tag="VIO83_11335"
     CDS             16007..16216
                     /locus_tag="VIO83_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY8331904.1"
                     /translation="MAGRETGTVKWFNDAKGYGFIQRESGPDVFVHFRAIRGEGHRSL
                     IEGQRVEFEVAHGQKGLQAEDVSKL"
     gene            complement(16348..16764)
                     /gene="tnpA"
                     /locus_tag="VIO83_11340"
     CDS             complement(16348..16764)
                     /gene="tnpA"
                     /locus_tag="VIO83_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEY8331905.1"
                     /translation="MSIENDIRRGRHCVFMMHAHLVFVTKYRREVFTQAILDDLRDVF
                     ASVCADFEAELVEFDGEDDHVHLLVNYPPKVAISNLVNSLKGVSSRMIRRKNYPSIRK
                     KLWGGALWSPSYFAGSCGGAPIEVIRQYIEQQQTPH"
     gene            16788..17999
                     /locus_tag="VIO83_11345"
     CDS             16788..17999
                     /locus_tag="VIO83_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8331906.1"
                     /translation="MQRLQAFKYELMPTGEQQCQMRRFAGSCRFVFNQALALQQANRE
                     AGGKYIGYVAMAKQLTAWRNSGETAWLKEAPVHPLQHALKDLEKAYKNFFEKRADFPR
                     FKRKGQRDSFRYPDPKQIKLDQANSRIFLPKLGWLRYRNSREALGELRNVTVSLSGGR
                     WFVSIQTRREVEIPVPQGGAVGIDLGIARFATLSDGTFHEPLNSFKRHEAALCQAQQA
                     MSRKTKFSSNWMKAKARVQHLHARIGNARRDYLHKTSTAISQNHALVFVEDLQVRNMS
                     QSAAGTTETPGKNVRAKSGLNKSILDQGWFEFRRQLDYKLAWSGGHLVAVPPRNTSRT
                     CPSCGHVSAENRRTQADFHCVECGFAENADVVGAINVLRAGHARLACEVSGAVRPPAA
                     GTRRGESALTG"
BASE COUNT         3442 a         5714 c         5802 g         3115 t
ORIGIN      
        1 tgcacgctgg ccggtctgtt cgtcggcgac ctgctcggcg tgctcctcgg tgtgccctcc
       61 aacgtcggcg gtgtcggcat cgccatgatt ctgctgatct tcggcggcag ctacctcagc
      121 aagcgcggcc tgctcgccgg caagaccgag cagggcatcg agttctggag cgccatctac
      181 attcccatcg tggtcgccat ggccgcccag cagaacgtgc tgggggcgct gtccagcggc
      241 ccggtggcga tcctcgccgg tctggctgcg gtcagcatca gtttcgccct ggtgccggtg
      301 ctcgaccgcc tcggccgcaa ggacgaggta gccccggagc ctgacgccaa gcccgtaacc
      361 cctgctaccg ctcctgtcgt aacccctgcc acctccagcg cccaacggtg attgccatga
      421 tcctcgaatc cctgaccaag gtactcaacg gctacggcat gatcagcggc ttcgccgtga
      481 tcggcgcgac catgtggctg tcctactggg cgtccgccaa gctcaccaag ggccgtctgc
      541 acggctcggc catcgccatc ctcgtcggcc tggtgctgtc ctacgtcggc ggcctgtaca
      601 ccggcgggca gaagggcctg gtcgacatcc cgctgctgtc cggcatcggc atcctcggcg
      661 gcgccatgct gcgcgacttc gccatcatcg ccaccggctt cggcgtcaat cccgcggagc
      721 tcaagcgcgc cggggccagc ggggtgctgg cgctgatcgt cggcatcttc gcctccttcg
      781 tcgccggcgc cggggtggcc ctagccttcg gctacaccga cgcggtcagc ctgaccacca
      841 tcggcaccgg cgcggtgacc tacatcgtcg gcccggtgac cggcgcggcc atcggcgcca
      901 gctccgaggt gatggcgctg tcgatcgccg ccggcctgat caaggccatc gtggtgatgg
      961 tcgccacccc cttcgtggcg cccttcatcg gcctcaacaa cccgcgcgcg gcggtgatct
     1021 tcggcggcct gatgggcacc tccagcggcg tggccggcgg cctggcggcc accgacccga
     1081 agctggtgcc ctatggctgc ctgaccgcgg ccttctacac cgccctcggc tgcctgctcg
     1141 gcccgtcgct gatctacttc gtgatgcgtg cggtgttctg atccctgtcg atgccggaat
     1201 gccagcggcc cgccttgtgc gggccgttgg cgttgcggga ggaagtgtgc gcagcgcttg
     1261 tccgccgcac agtgtctcca ccagagggca aagtctacgg tcgaggttca cgaactacac
     1321 agcaatgagc tattgcatga tgtaaagtga tgcacattta tgcttcgtgg tgaatcggca
     1381 tgaagtccgc acgtacaact atctctctgc cccccgagca gcttgagcaa ttgcaggctc
     1441 tggctgatcg tcacggcctg tcgctggcct gggtcatccg ccaggccatc ggcgaattcc
     1501 tcgcccgggt cgagcacccc gccaactttg acccccttgc atccaggaag gagagttgat
     1561 gtacaccccc tatcagcgcg cctatctggc ccactggctg tcgctgtcca gaggcgagga
     1621 gcaggcgctg acgcaaacca tcgccagcgc caaggtcgac atgaacccgc atcaggtcga
     1681 tgcggcgctg ttcgcgctgc gttcgccgtt atcccagggc gtgttgctgg ccgacgaggt
     1741 cggtctgggc aagaccatcg aagccagcct ggtgctggcg cagaagtggg cggagcaacg
     1801 gcgcaagctg ctgctgatcg ttccggccac cctgcgcaag cagtggagcc aggagctgga
     1861 agacaaattc gccttgcctg ccgtcattct ggaagccaag aacttcaacg aggcatgcaa
     1921 gcgtggcgtg ggcaatccgc tcgatatcga acaggcgttc ggtcgcccgg ccatcgcgat
     1981 ctgctcctac gaatttgccg cgcgcaagca gcgggaattg gggctgatcc cctgggatct
     2041 ggtggtcttc gacgaagcgc acaagatgcg caacatctac aaaggcgaag gcgccaaaac
     2101 cgccaaggcc ttggatgaag ccttgaaagg ccgtaccaag gtgctgctct ccgccacgcc
     2161 catgcagaac aatctgctgg aactgtacgg cctggtgtcg atcgtcgacc cgcacttttt
     2221 cggcgacctg gaatccttca aggctcgcta cagcaagcct cggctgtcgc aagcggagct
     2281 ggaccaactg cgccagcggc tgaaaagtat ctgcagccgc acgctgcgac gccaggtgca
     2341 ggaagagggc ggcatcaact tcacgcgccg ctactcgatg accgagccct acgatccgca
     2401 gccggacgag caggagctgt accgcaaggt ctccagctac ctgcaggacg aaagcgtgct
     2461 ggcgatcaag cccaacgccc gtcacctggt gaccttggtc attcgcaaaa ttctggcctc
     2521 gtcctcccac gccatccagg gcaccttgca gcgcatggtc gagcgcctgg aaaagcgcat
     2581 gtcactggcc gatgccctga gcgattacga ggaactggac gatctggccg acaccagcgg
     2641 cgtggacgag gacgactttg tcgacccggc agttctacag gcggaaatcg agcggctgaa
     2701 agagtttctg aatctggccg ccagcatcga gcgcaacgcc aagggggacg cgctgctgac
     2761 ggtattgggc agatcctttg atttcgccga gcgcctgggt ggccagcgca aagcggtgat
     2821 cttcaccgaa tcggtacgca cccaggcctg gttgttcgag ctgctcagca gcgagggcta
     2881 tcgggacaag gtcgtactgc tcaacggcag caacagcgat ccggagtcgc tgcgcaccta
     2941 tcaggcctgg ctggaaaagc atcaaggctc ggcgcgggtt tccggctcga agagcgccga
     3001 tatgaaggcc gccctggtcg agcgcttccg tgagcaggcc agcatcatga tctgcaccga
     3061 agccggcgcg gaaggtatca acctgcagtt ctgctcgctg ctgatcaact acgacctgcc
     3121 ctggaacccg cagcgggtcg aacaacgcat tggtcgcgtg caccgttatg gccagaagca
     3181 cgatgtggtg gtggtgaatt tcctcaacaa cagcaaccgc gccgaccagc gggtctatca
     3241 gttgctcagc cagaaattcc agttgttcga aggcgtgttc ggctcgtccg atgaaatcct
     3301 cggcgccatc gagtcgggcg tggatatcga gcagcgcatc cacgccattt accagaagtg
     3361 ccgcagtgac gaggaaatcg aggagcaatt caaccagttg caggagcaac tgcaggcgca
     3421 gatcgaaacc cgcaatcagg atgcgcgccg ctcgctgctt gaacacttcg acgtcgacgt
     3481 ggtggcccgc ctgaacatgt gccgcgaacg cacccaacag gcgctggatg actaccagca
     3541 acgcctgctg ctgctggcgc gtatgagcgt ccctcaggcg cgcttcgaag gcgagcgctt
     3601 cgagctggat ggccactggt acgacccgaa ctggcagcgt gcgcaagaga ccgacgcccg
     3661 cttcttccag ccgaacgacg gcaagggcgg tgaactgatc gagcgggcca aggcgacgtt
     3721 gagcgccccg gcggcggctg aactgctgct gcgctacgtc ccggcggaga acggccagtt
     3781 gtccgatgtc cttgaattgc tcggtcagtc gggtgagctg gtggtggaga agctcaccct
     3841 gagcaccgcc acgcagcggc gcgagcacct gttgctggcc ggctttacgg attccggcca
     3901 gttgctggac gagcgcacct gcgaccgctt gctgaggctc aacgcccagg aagtctcgac
     3961 gcaaccggct cttcagcatg tggcaaccct gcaggcgcgg ctcgacgcct tgcgcggcga
     4021 atgcttgcag caggcgaacg cccacaacga acgctacttc gaagaggaaa ccgaaaagct
     4081 cgaacgctgg gccgaggaca agcgcatcgg cctggatatt cgcatcaagc agctcgacca
     4141 agagatcaag gaagcgcgca aggctgtgcg tcagctggaa accctgcagg aaaagatgag
     4201 cgccaagaag gcgctgaaaa aactcgagcg cgagcgcgac aacctgatgc tcgactacca
     4261 cgaagaaaag aagcacatcg aagccgagga agaccgcctg ctcgaagcaa tcgagcagaa
     4321 cctggaaatc agcgtgaaac atgaacgctt attcagcctg cgctggcgcc tggagggcac
     4381 ggcatgatca acaccacgga attggtacgc gagctgatct accagatccg ccttggcgaa
     4441 gactcggcct acgagttcaa ggcggtgacc cttcacgaca acaaagtcac caagccgcac
     4501 cgtgacagcg tggccgacga gctggccgcc tttgccaacg gcagcggcgg ctttctgatc
     4561 ctcggcgtca acgacaagac ccgcgaagtc gagggcatcg cactggagca actggatacc
     4621 gtcgagctgt ggctgaccaa catggccagc caggccatcg atccgccgct gcccatcgaa
     4681 acgcgcctgg tggaaattcc cgaccatcag ggcaatgcca gaccggtggt ctgggtgcgc
     4741 gtggcgaaga gcctgttcgt ccatcgcagc ccgcatggct acttccaccg cgtcggttcc
     4801 agcaagcggg aaatgtcgcc cgagctgctc gcccggctgt tccagcaacg cagcatggcg
     4861 cggctgatcc gcttcgacga acaactggtg cccaatgcca gcagggacga catcgatccc
     4921 gagctgaagg cccgcttcct gcgcagtgac ctgccggaag ctctgcagct gaaaaaactc
     4981 tatctgctgc tggaagacga aaatcgccag ccgcacctga ccgtcactgg cgtgctgctg
     5041 ctcacccgcc gcccggcgga tttcctttct tcggcctatg tgcagtgcgt ggcctattca
     5101 ggcagcgagc gcagcgccga gtaccagctg gacgcccaag actgcgacgg cccggtcgac
     5161 cagcagatcc agcaggcttt cgccttcgtc aaacgcaaca tgcgcgtcca ggcggtcaag
     5221 cgccctgggc gcatcgatat tccgcaatac gacctaggcg cggtctacga ggcgctggtc
     5281 aatgccgtgg cgcaccgcga ctacgctatg cacggcgcgc gcattcgcgt gcacctgttc
     5341 gccgaccggc tggaaatctc cactcccggc gggctgccca acagcctgac ggtggagagc
     5401 atggatgcca acagcatcag ccgcaacgaa accctggtga acctgctcag ccgctattac
     5461 ccggccgatc cggctacccg ccggcagaac atgatcgaac gccgcggcga aggtgtgccc
     5521 accatcatcg ccgccagcga gcggctgtcc ctgcgccgcc cgctctacga gcagatcgac
     5581 cataccgaac tgaaactgac catctacgcc gccagccgcg aacgcaacgg cctggtggcg
     5641 cagtttgccg acgacgaata aggctgtacg caatgagcga aaaacagaag ctggaactga
     5701 cctggatcgg caaggacaag cgcccgcgcc tggagccgcg catcctgatc gaggacaagg
     5761 cgctgtccta ccgcgccgaa gccattgcgc ccctgcaggg cgatctgctg gccggcgagc
     5821 agccgccgag ctacaacgac aacctgctga tccagggcga caacctgctg gcgctcaagg
     5881 cgctggagtc gcagtacgcc ggcaaggtga agtgcatctt catcgatcca ccgtacaaca
     5941 ccggcagcgc cttcacccac tacgacgatg ggttggagca ttcgctgtgg ctgacgatga
     6001 tgcgcgaccg actggaaatc atccgtactc tgttggcgga cgatggctcg ctgtggatca
     6061 ccatcgacga caatgaagcg cactatttga aggtgttgtg cgatgaggtg tttgggcgtc
     6121 ggaattttgt ggccaatatt gtttggcaaa agcgcacatc aagagaaaac cgggctgcag
     6181 tcggatcggc ccatgatcat ttgcttatat atgcaaagtg tggggcagta gattggaagt
     6241 ccacccggta gcttttgcca gcaaacaacg cagggtacgc aaatccagac aatgaccctc
     6301 gcggtgagtg gcgctctatt ccattcagtg cgcaaggtta tcgcccgaac caaatgtata
     6361 gaatcaaaac ctcgagtggc gattttgttg atcctcccaa aggacggtgc tggggtgcaa
     6421 ccgagcctgt ctacttggag tatctgagag acaatcgggt ttatttccca aatgggggta
     6481 caggaaaacc cagaatcaag cagttccgag gagaagaaaa aggccttgtg ccaatgactt
     6541 ggtgggatgc tgaatttgcc ggcgacaatg aggccgcaaa aaaagagatc ctggctctat
     6601 ttcctgacga tgagccgttt ggcaccccaa agccagagca gcttttagaa cgagtgatac
     6661 acatagccac tcgtcccggc gacctcgtcc tcgactcctt cgccggctcc ggcaccaccg
     6721 gcgccgtggc gcacaagatg ggccggcgct ggatcatggt cgagctgggc gagcactgcc
     6781 atacccatat cgtgccgcgc ctgaaaaagg tcatcgacgg tgcggaccag ggcggcatca
     6841 gcaaggcggt cgactggcag ggcggcggcg gtttccgcta tctgcgtctg gcgccctcgc
     6901 tgctgaagaa ggaccagtgg ggcaactggg tgatcaaccc ggcctacaac gcggagatgc
     6961 tcgccgaggc catgtgcaag catatgggct tcagctacgc gccgagccag acgcatttct
     7021 ggatgcacgg ccgcagcagc gagacggact tcatctacgt caccaccggc agcctgagcc
     7081 acgaccagtt gcgcctgatc agcgaggaag tcggcagcca gcgcagcctg ctgatctgct
     7141 gcaaggcgtt catggccgac accgaagcct ttccgaacct gaccctgaag aagattccca
     7201 aggcgattct cggcaagtgc gagtgggacc acgacgacta tagcttcagc atcaacctga
     7261 cggctgaaga cgcgagcgaa gagccgctcg aagacgaata acggaaagga aatctgtcat
     7321 ggctgagccc atcaacggcc agaaagtagc caaccaagtg accgcgcgcc tatcgctgcg
     7381 caagccccag cgcgaatcgc tgcaagtgct ggtcgatctg ctcgaccagc tgcctttggg
     7441 caaggagccg gacctgcagc acttgctgca ggtgattcag gcccactacc cgagcatgca
     7501 ggccttcgag cgggactttc cctcgctgtg cttcgccctg gccaccgggg tgggcaagac
     7561 tcgcctgatg ggagcgatga tcgcctggct gttcatcacc ggccgtagcc ggcacttctt
     7621 ggtgctggcg cccaacctga ccatttacga gaagctgaag aaggatttca ctctcggcca
     7681 gcccaagtac gtgttcgccg gcatcccgga aatggccaac accccgccgg tggtgatcac
     7741 cggcgaggac tacgaggacg gccgcggcgt gcgcctggac tatgccgtgc cggcaatgct
     7801 ctccggcgac ctgttcggcg ccgacgccag cctgcacatc aacatcttca acatcgccaa
     7861 gatcaatgcc cgcgacaaca agaagggcgc ggtcaagtcg gccaccgcca aggtgcgccg
     7921 cctgcaggag tatctgggcg agtcctactt cgactacctc gccgcactgc ccgatctggt
     7981 agtgctgatg gatgaggcgc atcgctacta cgccgatgcc ggcgccaagg cgatcaacga
     8041 cctgaagccg gtactgggcc tggagctgac cgccacgccg aagaccgtcg gcgccaagcc
     8101 acgcccgttc ggcaatgtgg tctaccacta cccgctgtcc agcgcgctca aggacggcta
     8161 tgtaaagatc ccggcggtcg ccacgcgcaa ggactttcgc gccgagcagt acagtcagga
     8221 cgcgctggaa accatcaagc tggaagacgg catccaccac cacgagtacg tcaaggtgga
     8281 gctggaaaac tacgcccgca gccagggcgt ctcggcggtc aagccgttca tgctggtggt
     8341 ggcgcaggac accacccatg ccgccgccat ccgtgcgcgc atcgagtcgg aggatttctt
     8401 ttccgggcgc taccggggcc gggtgatcga ggtgcactcc aaccagtccg gcgaggagtc
     8461 ggacgagtcc atgcagcgtc tgctggcggt cgagcacgac gaagccaccg aggtggtcat
     8521 ccacgtcaac aagctcaagg aaggctggga cgtcaccaat ctctatacca tcgtgccgct
     8581 gcgcgcctcg gcctcggaga tcctcaccga gcagaccatc ggccgcggtc tgcgcctgcc
     8641 ctacggcaag cgtaccgggg tcgaggcggt ggatcggctg tgcatcatcg cccacgaccg
     8701 tttccaggag atcatcgacc gggccaacaa ggacgactcg ctgatccacg agaaaatcta
     8761 catcggcagc ggcgccgatg ccgacgtgcc ggatcgccag ccgcagcagg tcgaggcttc
     8821 tcccgtatcg gtggtacaga cctccagaga agacgctacc ccgacggccg aaaacgccgg
     8881 ccgctatcag gtggcggtac gctcggagca ggaggctaag atcgctgcgg cggctatcca
     8941 ggccgttcag gaggaggcgc gcaagctgtc ttccagtctg cagttgggcg acgaggacgt
     9001 gcagcagaag ctggtccgcc gcgtacagcg tgccctgcag gaacagctac ccgtccagca
     9061 gcaactggct gggctggaaa gcgaggcgct gcccagcgcg gcggaaatcg aaagcctggt
     9121 gcgcagcgtg acggaaaagg ccgttgagct gaccatcgac gtgccgcata tcaccgtgct
     9181 gcccactcgc gaagtgaact acggcttcca aaatttcgac ctgcagaatc tggccagcat
     9241 caagttggag ccggtcagcc aggaaatgct cctgcgccat ctggagaacg accacagcgc
     9301 gagtatccag tggggccgac aggaagctcg cgagccgcga ctggaggact acctggtgcg
     9361 tcacctgttc acccgagatg aggtggatta cgaccagtat gccggcctgc tgtacaagct
     9421 ggcggggcag gtaatcgcgc atttgcagag ctacctgaaa gactcggacg aggtcgaaaa
     9481 cgtgctgctt tactggcaga agcagctcag cgacttcatc tgggcgcaga tgcaggcgca
     9541 gcgctggaca acgccctgcg actatcgcgg caaggtgatt gcgggcttcg atgtgctccg
     9601 tccggccacc tttaccctgc ctgccggcga caaaccccgc catttccgca ccccggtcga
     9661 agataaacgg gcgattcggc agatggtgtt cgaaggcttc aagcgttgct gcttccccta
     9721 ccagaaattc gactcggtgg atggagaatg gcgtctggct caggtgctgg aagacgacga
     9781 caaggtgctc aagtggatga aaccagcgcc aggccagttc cgcatagagt atctggatgg
     9841 tcggaactac gagccggact ttgtggtgga gactgagtcg ggctatctga ttatcgagcc
     9901 aaagcgagcc gatcagatcg gccatgagga ggttcgggcc aaagcccgtg cagctatgcg
     9961 ctggtgcggt ttcgccaacg aacacgccca gagcaacggc ggcaaacctt ggacctacct
    10021 gctggtgccg gatgaccaga tcgtgctcgg tgtcagtgtt gcgcgtctgg tgacggagtt
    10081 tgcgaccaat ctgccaggtt gatagtcaac ccagtctgct ggggtgttga tcaaccctca
    10141 tgccgcttgg catacatccg acactcggcg agcagcgcca gcaggttggg gtcgcgctcc
    10201 ttggccttga ggaataccag gccgatctgc tgctgcatcc ggtagcgtgc ctgcagcgga
    10261 atcagccgca cgcggttttc gtacaccgca gcgatgcgtc cgggcagcag ggcgtagccg
    10321 accccggagc tgaccatgct gagcagggtg aagatgtcgt tgacctgcat cgccaccttg
    10381 ggcgcgaagc cggcctgtcg gaacacccgc tgggcgtcct ggtgggtggc gaagccctgg
    10441 gacagggtaa tgaaggtggc ctcgcgcagc tcggccaggt ccacctccgc ctggccggcg
    10501 aacggcgagt cggtgggcac ggcgaggaag atgtcgtcgc aaaacagcgg cagcgactcg
    10561 cagtccggat cgctcacgct gtcgttgagc gacaccagga tggcgtctac ttcgaggttc
    10621 ttcagcttgt acagcaggtc gacgttggag ccgaggatca ggtcgatgtt cagttcgctg
    10681 cgccgcagct tgaggcccat gatcagctgc ggcacggtct tcaccgtcag cgagtagagc
    10741 gcgccgagct tgaagcgctc ggcggagaag ccggccgcct cgcgggtcag gcgcacggtg
    10801 tcgagcatgt cggcgatcag cttctgcgcg cggtcctcca gcacatgggc gctttccagc
    10861 ggggtgagct tgcgcccctc gcgcttgaac agcggacagc gcagggcgtt ctccagcgaa
    10921 tggatggcgc ggtgcacgct gaccgtgctg gtgtccagct cggcggcagc gcgcgacagg
    10981 ttgccgctgc gcatgaaggc caggaagatt tccagcttct tgaaggtcag ttcttcgtcg
    11041 atctgcatga tggcgttcgg tggcgacggt gcccccgatt gtgccgcaaa gccggtgggc
    11101 gtgccctcac tctccgcggt tgtgtcaccg gccggcaggc cgctcgggcg attccacaat
    11161 tgccgtcgcc gttgccgccc cttgcattac gcgcacgctc tacctagagt tcagccggca
    11221 ttcgagacgc ctccgtggcc gtctggcacg gaggctccag caggttatgg agtggccatg
    11281 atcgaaatag aaaaactcac caaacgcttt gtgcagcaca ccgtggtgga cgacctgtcc
    11341 ttccgcgtcc agcccggcga ggtgctgggt ttcctcggcc ccaacggggc cggcaagtcg
    11401 acaaccatga agatgctcac cggctttctc gcgccgaact cgggctctgc gcgcctgttc
    11461 ggcttcgata tccagaagga caccctcaag gcccagcggc tgatcggcta tctgccggag
    11521 ggcgcgccct gctatggcga catgcaggtg cgtggcttcc tcgagttcat cgccgaggtg
    11581 cgcggttacc gcggcgccga gaagcgctcg cgggtggcca gggcggtgga gcaggtggag
    11641 ctgcaggcgg tgctcggcca gtcgatcgac accctgtcca agggcttcaa gcgccgggtc
    11701 ggcctggccc aggcgattct ccacgatccg cgcgcgctga ttctcgacga gcctaccgac
    11761 ggcctcgacc cgaaccagaa gcaccaggtg cgccagctga tccagaacct ggcgaccgac
    11821 aagatcatca tcatttccac gcacatcctc gaggaagtca ccgcggtttg tagccgcgcg
    11881 gtagtgatcg ctgccggccg gctggtggcc gacggcacgc cgttcgagct ggaaagccgt
    11941 tcgcgctacc accaggcggt gaccctggtc ggcgatgcgc cgcttgacga ggtcgcgctg
    12001 gcggcgctgc ccggcgtggc cggggtggag cgcaacgccc gcgagcacag cctgaccgtg
    12061 ctggcgcagc cggggcaggt gatcttcccc cacgtcagcg cgctggtggc ggcgcgcggc
    12121 tggagcatca aggaaatgga cgtggagcgc ggccgcctcg acgaggtgtt ccgcaccctg
    12181 actcgaggag aggcggcatg aggcagttgc cggtagtctt caagcgcgag ctggcgagct
    12241 atttcgccac gccgctggcg tacgtgttca tcgtgatctt cctggcgctg tccggggtgt
    12301 tcacctttta tctgggcagc ttcttcgagc gcggccaggc cgatctcggc gcgttcttca
    12361 gcttccaccc ctggttgtac ctgttcctgg tgccggcgct ggccatgcgc ctgtgggccg
    12421 aggagcgcaa gagcggcacc atcgagctgc tgatgaccct gccgatcacc cgcgccgatg
    12481 cggtgggcgg caagttcctc gccgcctggg cgttcgccgg catcgccctg ctgctgacct
    12541 tcccgatggt gctgacggtc aactacctgg gcgagccgga caacggcgcg atcctcaccg
    12601 gctacatcgg cagctggctg ctggccggcg gctacctggc catcggctcg tgcctgtcgg
    12661 cgctggccaa gaatcaggtg atcgccttca tcctcaccgt ctgcgtgtgc ttcctgttcg
    12721 ttgccagcgg cttccctctg gtgctggatg ccttcagcgg ctgggcgccg caggcgctgg
    12781 tggatgcggt ggcgtcgttg agcttcctga cccgcttcga tgcgatcagc aagggcgtgc
    12841 tcgacctgcg cgacctgctg tatttcgcca gcctgatcct ggcctggctg acggccaccg
    12901 cggtggtgct cgacctgaag aaagccgact gaggacgccc gatgaaacgc atcatttatt
    12961 ccggcaccgg gctgctgttg attgccctgg tgttcctggc cttcaacatg gtcaccgggc
    13021 tggggctgca taacgcgcgc ctggacctga ccgagcagaa gctctacacc atcagcgaag
    13081 gtaccgagcg catccttggc cagctcgacg agccgatcaa cctgtacttc ttctactccg
    13141 acaacgtcag ccaggatctg gtgccgctgc gcaactacgc caagcgcgtc gaggaaatgc
    13201 tgcacgccta cgagcgcgcg gccggcggca agctgcacct gaaggtgatc gaccccgagc
    13261 cgttctccga ggacgaggac cgcgccgccg agttcggcct gcagggcgtg ccgctgcagc
    13321 agggcggcga caagctgtac ttcggcctgg ccggcaccaa cgcgctggac aatgtgcaga
    13381 gcatcccgtt cttcccgctg gatcaggagg agttcctcga gtacgagatc agccgcctga
    13441 tccagagcct ggccaagccc gagcggccgg tggtcggcgt gctgtccggg ctgcagatgg
    13501 acggcagctt caatccgctg accggccagg ccggtacgcc ctggatggtg atggaggaga
    13561 tccgccagca gttcagcatc aagagcctgc gcaacgacag cgaccggatt cccgatgagg
    13621 tgtcggtgct gctgctggtg catccgaagg atctgccgcc gccgacgctg tatgccatcg
    13681 accagttcgt cctgcgcggc ggcaagctgc tggctttcgt cgacccgttc agcgagagcg
    13741 acaacagcgg catgttgggc aatggccaag gcaacgcctc tgatctcgag ccgttgttca
    13801 aggcctgggg cgtgcgtctg cgggcggggc aagtgctcgc tgacggcacc tacgcgatgg
    13861 ccgtcggcgt gggcgtgggc gaagagcagc gcccggtgcg ccatccggcc tggctcggcc
    13921 tgccgaaggc ggcgctggac cagaccgacg tgaccaccgc ggcgctggag aacatcaccg
    13981 tggccagcgc cggcatcctc gagccggtgg agggggcgaa gacccgtttc accccgctgc
    14041 tgcagagctc cgagtacgcc atgccgttcg acgcccagcg cctggctggg ctgcgtgatc
    14101 cgcaggagct gatgcgcgag ctggagccga ccggcgagcg ctatgccgtg gcggcgcggg
    14161 tcagcgggcc ggcgccgtcg gcttaccccg agggcatcga ggggcagaag gacggcctga
    14221 agagcgctga gagcatcaac gtcatcgtcg tcgccgacac cgacctgctc agcgaccgca
    14281 tgtgggtgca ggtgcaggag ttcttcggcc agcgcgtgcc gcagccgttc gccgataacg
    14341 ccggcttcgc catcaacgct ctggacaacc tggccggcag cgatgcgctg atcagcgtgc
    14401 gttcgcgcgg gcgcttcagc cggccgttcg tggtggtgga gcggctgcaa cgcgaggccg
    14461 aggcgcgctt ccgcgagcag gaagaggtgc tcaagcagca gctggcgcag accgagcagc
    14521 gcctcgccga gctgcagcag agtgacagtc ccgagcaggt gctcgagctg acgcccgagc
    14581 agcaggcgac cctgcagcag ttcatgcagg acaagctgaa gatccgcaag gagctgcgcg
    14641 aagtgcgtta ccagctcaat gccgacatcg aggggctggg acgggtgctg aagttcgtca
    14701 atattgccct ggtgccgctg ctgctgaccc tcggcgtcat cgtgctgtgg ctgttccgcc
    14761 gccgtcgggc ttgaggaggt cgtcatgcgt cgttccgcat tgctggttct gctgctgatc
    14821 gcgctgggac tgggtggcct gttcgcctgg ctgcaacgct ctgccgaggt gccgcagcag
    14881 gccgctcggc aactgttgct gccggtgctg gccgggcatc tcgagcaggt gcgtgcgctg
    14941 gagattcgcc acggcgatct gccggcgatc cgcatcgagc gcgccggcgg cggctggata
    15001 gtcccggcca aggccggcta tccggcggcg gccggcgagg tcaaccgcct gctgcgggcg
    15061 ctggccgagg cgcacagggt cgaggccaag acgcgcaacc cggacaacca tgcccgcctg
    15121 gacctggcgg agcgaggcga gggcgcggct acgcgcctga ccctggaggg cgtgggcaag
    15181 acgcccgtgg tggtgctgat cggccaggct gcgcgccagg gcgggcagct ggtgcgcctg
    15241 gccggtgacg atcaggtctg gtcgatcgac cagtcgctgc agttggtgga caacgagctg
    15301 gcctggctgg atcggcgcat cagtgcgatt cccttcgcca gcgtgcgcga gctggaggtg
    15361 cgccatgccg acggcgagca gctgaacgtc tggcgcgcaa ccccggagca ggccaacctg
    15421 gcgctgcgcc agttacccaa gggccgcacc ctggcctacg agccggtagc caacggcatg
    15481 gcggcgctgt tctcggcgct cgatttcgcc gacgccgcac cgctggcgca ggtcggcttc
    15541 aaggcgcggc cggagctgga gtttagcctg cagaccctcg atggcggcga actgcacggt
    15601 gccttccacc agcagggcgg tcagcactgg ctggtgctgg gcgacagcaa agggctcgac
    15661 ggccgcctca tcgccggcaa ggactgggcc tatcgtctgg aggagcagca gtaccgcacc
    15721 ctggccaaac gggtgaagga tctgcttggt ccgcagggct gacggttggc aatgaccggg
    15781 acccgccgcc cggcctttgt cctgggtcac agcgcggggg cttttcagca caggcgcttt
    15841 gtttatactc gggccagcgg tcgtcaagaa cgcgattcaa aatacccact ccagacgcct
    15901 cgcggcggcg gaggccggat ataacaagaa gaacgtttaa ccacgtaccg tcccgagctg
    15961 ctcccgcagg gagcattgaa actgtgcaag tctggagaga tgtggaatgg ctggacgtga
    16021 aactggaacc gtcaagtggt tcaacgatgc caagggctat ggcttcattc agcgcgaaag
    16081 cggtccggat gtgttcgtgc acttccgtgc gatccgtggc gaaggccacc gctcgctgat
    16141 cgaggggcag cgcgtcgagt tcgaagtggc ccatgggcag aaaggcctgc aggcggaaga
    16201 tgtttccaag ctctgaatgc tgatatctgc aaagcaaaaa cccgtccttg cgacgggttt
    16261 ttcgcgcttg atccagtgcg cggcaaacct cgcacttcag ggcggggaag gatagcgcgg
    16321 acggcgtagc cgtccccggt tttgccttta atgaggcgtc tgctgctgtt cgatgtactg
    16381 gcggatgact tcgatgggcg cacctccgca gctgccggca aagtagctgg gcgaccacag
    16441 cgcgccgccc cacagctttt tgcggatgct ggggtaattt ttcctgcgga tcatgcggct
    16501 agagacgcct ttcaggctgt tcacgaggtt ggagatggcg accttcggcg gatagttcac
    16561 cagcaggtgc acatggtcat cctcgccatc gaactcaacc agttccgctt caaagtcggc
    16621 gcatacgctg gcgaaaacgt cgcgaaggtc gtcgagaatg gcttgggtga agacctcgcg
    16681 ccgatatttc gtaacgaaga ccaagtgggc atgcatcatg aagacacagt gtctgccgcg
    16741 cctaatatcg ttttcaatgc tcatagacca agagtatagt gctggacatg cagcgactcc
    16801 aagccttcaa atacgaactg atgccgaccg gcgaacagca gtgccagatg cgccgcttcg
    16861 ccggctcctg tcggttcgtg ttcaaccagg ccttggcgct gcagcaagcc aaccgcgaag
    16921 cgggcggcaa gtacatcggc tatgtggcga tggccaagca gctgacagct tggcgcaaca
    16981 gcggcgaaac cgcctggctg aaagaggcgc ccgtccatcc actgcaacat gcgctcaaag
    17041 acctcgaaaa agcctacaag aacttcttcg agaagcgcgc cgatttcccg cgcttcaagc
    17101 gcaagggcca gagagacagc ttccgctatc ccgaccccaa gcagatcaag ctggatcagg
    17161 ccaatagccg cattttcctg cccaagctcg gctggttgcg ctatcgcaac agtcgcgaag
    17221 cgctgggcga gctgcgcaac gtcaccgtca gcttgagcgg cggccggtgg tttgtgtcga
    17281 tccagacgcg gcgcgaggtg gaaatccccg tgccgcaggg tggtgcagtc ggcatcgacc
    17341 tggggatcgc ccgcttcgcc accttgagcg atggcacgtt ccacgagccg ctcaacagct
    17401 tcaagcgcca cgaagccgcg ctgtgccagg cgcagcaggc catgagccgc aagacgaagt
    17461 tcagcagcaa ctggatgaag gcgaaagccc gcgtccagca cctccatgcc cgcatcggca
    17521 atgcccgccg cgactacctg cacaagacct caaccgcgat cagccaaaac cacgcgctcg
    17581 tgttcgtcga ggatttgcag gtacggaaca tgtcccagtc ggccgccggc acaaccgaaa
    17641 caccgggcaa gaatgttcgg gccaaatccg gcctgaacaa atccatcctc gaccagggct
    17701 ggttcgagtt ccgccgccag ctcgactaca agctggcgtg gagcggtggt catctggtgg
    17761 cggtgccgcc ccgaaacacc agccggacct gcccgagttg tgggcatgtg tcggcggaga
    17821 accgtcgcac ccaggccgat ttccactgcg tcgagtgtgg tttcgcggaa aacgccgatg
    17881 tggtcggcgc gatcaatgtg ttaagggcgg gacacgcccg gttagcctgt gaagtgagtg
    17941 gtgcagtcag gccgccagca gcaggaaccc gccgaggtga gtcagccctg acgggctgaa
    18001 cacggtagga atccccttcc tttccgccct tgggcggtga gcgtcagcga gagggagggg
    18061 gggatgtcaa ggc
//
LOCUS       DATCUN010000292        22571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_52307, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000292 DATCUN010000000
VERSION     DATCUN010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 22571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22571
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52307"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /gene="nagZ"
                     /locus_tag="VIO83_11350"
     CDS             complement(<1..713)
                     /gene="nagZ"
                     /locus_tag="VIO83_11350"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY8331907.1"
                     /translation="MSGSLMLDIAGTWLTAEDRRLLRQPEVGGLILFARNIEHPQQVR
                     ELCVAIRAERPDLLLAVDQEGGRVQRLRQGFVRLPSMQALAAAPDALRLAGHCGWLMA
                     TEVLAVGLDFSFAPVLDLDHGRSTVIGSRAFGSDPAQVSELATAFIAGMRSAGMAATG
                     KHFPGHGWPEADSHVAIPRDERSLDEIRACDLKPFVALAPQLDGIMPAHVIYPQVDEQ
                     PAGFSRRWLQDILRGELGYR"
     gene            complement(723..1241)
                     /locus_tag="VIO83_11355"
     CDS             complement(723..1241)
                     /locus_tag="VIO83_11355"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY8331908.1"
                     /translation="MLDLLHISIADQCLYGFAAGRLCLRLPVSTALNGPGEQQGSGCT
                     PRGRHQVRAKIGADLPAGAVLVGRRWTGEVWSAELAAQHPQRDWILTRILWLSGCEPG
                     RNRLGAVDTFRRYIYLHGTPDSEPMGEARSHGCIRLRNADLLALFEQVPPHCSVIIDE
                     APQPQWQAAALA"
     gene            complement(1306..1995)
                     /locus_tag="VIO83_11360"
     CDS             complement(1306..1995)
                     /locus_tag="VIO83_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY8331909.1"
                     /translation="MVESATVERILDAAQQLFAEKGFTETSLRLITAKAGVNLAAVNY
                     HFGSKDALIQAVFVRFLGPFCQRLEGELESREGKPATLEELLELLVALALEVSRGSSN
                     LRVFMRLLGMAVREHQSKLRNYLAGLYGKVFRRYLQLLQEAVPGIPPLELFWRVHFML
                     GAASFSMAGIEGLREIVARDFDVDVSAEQVLSLLVPFMAAGMRADSALTDPALIAARP
                     HRNGKANNHGK"
     gene            2168..2782
                     /gene="lexA"
                     /locus_tag="VIO83_11365"
     CDS             2168..2782
                     /gene="lexA"
                     /locus_tag="VIO83_11365"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY8331910.1"
                     /translation="MQKLTPRQGEILAFIKTWLDAHGYPPTRAEIAQELGFRSPNAAE
                     EHLRALARKGAIEMIPGASRGIRIPDSAPALREGELPIIGRVAAGAPILAEQHIEDSC
                     RISPEFFHPRADFLLRVHGMSMKDVGILDGDLLAVHACREAVNGQIVVARIGDEVTVK
                     RFQRRGKRVQLLAENPEFAPIVIDLEQPDQEFVIEGLSVGVIRR"
     gene            complement(2854..3093)
                     /locus_tag="VIO83_11370"
     CDS             complement(2854..3093)
                     /locus_tag="VIO83_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331911.1"
                     /translation="MPTSFLEIVELPDGRIVLRRGDDEEALVTLEFSEDAKAFLLGQH
                     IEVAKAMFNLGVQMAGSMADGDYEHEYSDEPRVLH"
     gene            complement(3244..3768)
                     /locus_tag="VIO83_11375"
     CDS             complement(3244..3768)
                     /locus_tag="VIO83_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6586 family protein"
                     /protein_id="HEY8331912.1"
                     /translation="MANELYTRTNQKLYFAGLALDAWRAADAAQALNARTQIQAAREG
                     ALFHLYGAVLGLCHEIAGYYRLPQAAAPQVEQLLTREVLDAAPSPELAELVDLALDRQ
                     SWLGQLLAAYRQLFEPPREAKKAKVDPALALITAVDVGGQAEEGELGLDSLVAWQQQI
                     KALATRFRDGMSEW"
     gene            complement(3926..6529)
                     /gene="topA"
                     /locus_tag="VIO83_11380"
     CDS             complement(3926..6529)
                     /gene="topA"
                     /locus_tag="VIO83_11380"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY8331913.1"
                     /translation="MGKSLVIVESPAKAKTINKYLGNQYVVKSSIGHIRDLPTSGSAS
                     KEPVKRGKAAASDAPVLTPKEKAKRQLFARMGIDPEHGWKAQYEILPGKEKVIEELRR
                     LAKDADTIYLATDLDREGEAIAWHLREAIGGDDSRYKRVVFNEITKKAIQDAFSQPGE
                     LDINRVNAQQARRFLDRVVGYMVSPLLWAKIARGLSAGRVQSVAVKLVVDREREIRAF
                     VPEEYWDVHADLATAKQAKVRFEVARQHGEAFKPVNEAQAMAAVAALKSAAYQVAKRE
                     DKPTSSKPSAPFITSTLQQAASNRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQD
                     AIDMARGFIEGEFGQQYLPAKPNVYSSKEGAQEAHEAIRPSDVNLKPTQLTGMERDAE
                     RLYELIWRQFVACQMPPAEYLSTSVTVKAGDFELRAKGRILKFDGFTRVQPPQSKPGE
                     DDVLPDMLQRDQLDLLQLDPAQHFTKPPARYSEASLVKEMEKRGIGRPSTYAAIISTI
                     QERGYVTLHNRRFYAEKIGDIVTERLSESFANLMDYGFTAGMEENLDDVAQGEREWKH
                     LLDEFYGDFKSKLEVAAASDSGMRANLPTLTDIPCKECGRPMTIRTASTGVFLGCSGY
                     ALPPKERCKATVNLIPGDEIAADDEGESESRVLRSKRRCAKCGTAMDAYLLDEQHKLH
                     ICGNNPDCSGYEIEEGQYRIKGYEGPSLECDKCGSEMQLKTGRFGKFFGCTNSACKNT
                     RKLLKSGEAAPPKIDPIKMPELRCAKVDDVYVLRDGASGLFLAASLFPKNRETRAPLV
                     AELIPHKDELDAKYHFLLDAPQQDPDGRPAVIRFSRKTKEQYVQSEVDGKPSGWRAFY
                     ADGKWTVEDKR"
     gene            complement(6653..6916)
                     /locus_tag="VIO83_11385"
     CDS             complement(6653..6916)
                     /locus_tag="VIO83_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HEY8331914.1"
                     /translation="MTLQTGLYRHYKGREYRVLGLARHSETEEELVVYQALYGDYGLW
                     VRPLAMFRETVEVEGETLPRFALVRSERVEFRYAEDDPAPPRA"
     gene            complement(6989..8164)
                     /gene="fadA"
                     /locus_tag="VIO83_11390"
     CDS             complement(6989..8164)
                     /gene="fadA"
                     /locus_tag="VIO83_11390"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HEY8331915.1"
                     /translation="MSLNPRDVVIVDFGRTPMGRSKGGMHRHTRAENLSAQLISKLLA
                     RNPLVDPAEVEDVIWGCVNQTLEQGWNIARMAALLTPIPHTSGAQTVSRLCGSSMSAL
                     HSAAQAIQSGNGEVFVIGGVEHMGHLPMTHGVDANPQLSLHVAKAAGMMGLTAELLGK
                     MHGISREAQDQFACRSHRLAHQATVTGLFRDELIPLEGHDEHGLLKVFDYDETIRPET
                     TVEGLAALRPAFDPKHGTVTAGSSSQITDGASCMIVMSGQRAMDLGLQPLARIRAMAV
                     AGVDPSIMGYGPVPATRKALQRAGLSLEDIDCVELNEAFAAQALPVLKDLKLLDKMDD
                     KVNLNGGAIALGHPFGCSGARISGTLLNVMKQRGATLGLATMCIGLGQGIATVFERV"
     gene            complement(8193..10340)
                     /gene="fadB"
                     /locus_tag="VIO83_11395"
     CDS             complement(8193..10340)
                     /gene="fadB"
                     /locus_tag="VIO83_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012313559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HEY8331916.1"
                     /translation="MIYQGKAITVQALEDGIAELRFDRAEESVNKFDRLTLSELGSAI
                     AEIRGNAALRGVIVTSGKEAFIVGADIGEFLGLFALPEAALLAANLETNGLFSAFEDL
                     PLPTVVAINGLALGGGLEMCLAADFRVMAEGARIGLPEVKLGINPGWGGTVRLPRLVG
                     ADNAIEWIASGKENDAQTALKVGVVDAVVAPARLREAALDLCRRAIAGELDHLARRQP
                     KLDKLKLNQIEQMLAFESAKGYVAGQAGPHYPAPLEAVKTIQKAAAHGRDKALEIESQ
                     ALVKLAKTEVARSLVGLFHNDQELKRKARGWEKQAREVELAAVLGAGIMGGGIAYQSA
                     VKGTPILLKDIREEAIQLGLGEAAKLLGQRVEKGRLTPAQMAAALNAIRPTLSYGDFA
                     EVDLVVEAVVENPKVKQAVLAEVEGQVRADTILASNTSTISINLLAKALQRPENFCGM
                     HFFNPVHMMPLVEVIRGEQTSAAAIATTVAYARKMGKTPIVVNDCPGFLVNRVLFPYF
                     GGFARLIAAGIDFQRIDQVMEKFGWPMGPAYLLDVVGIDTAHHAREVMAAGYPERMQD
                     DWRSAIDVLYEAGRLGQKSGRGFYAYQTDKGGKPKKVADPSVHELLKPVLGAPRELSD
                     EDIVAIMMLPLCLETVRCLEDGIVDSAAEADMGLVYGIGFPPFRGGALRYIDAIGVAA
                     FVALADHHVALGPLYQSTAGLREMARDGRTFFA"
     gene            10666..11106
                     /locus_tag="VIO83_11400"
     CDS             10666..11106
                     /locus_tag="VIO83_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8331917.1"
                     /translation="MTYQHLLIAVDLTDDCHKVIERAESLAGCCAAKLTLVHVIEPMS
                     MAFGGDVPLDLSALQEQQTEQARQQLEAFSAQHPQLGKENCRLLFGQPRQEIHRLAHE
                     LGCDLIVTGSHGRHGLALLLGSTSNDLLHGATCDVLAVHLPKPE"
     gene            complement(11160..11852)
                     /locus_tag="VIO83_11405"
     CDS             complement(11160..11852)
                     /locus_tag="VIO83_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331918.1"
                     /translation="MTIDYKITLDDNHEFNYRVELDRSYDPLQAGEAPRWTRLEHEQC
                     SNCPLNKDLHDHCPAAVDLYRVIEDFRGLPAFQKAKVRVITPEREYYKEVSLETGLRS
                     LLGVLMATSACPMLCRLKPMAQQHLPFASNQEFILRTVSLYLMRQYYNLREGRHADWE
                     LKGLIKNYQQLQLVNQAFWQRILATCEGDSNLKAFLSFFSMASSVSYSLEAQLSKLRP
                     LVMSSSSEYGLL"
     gene            complement(11879..13798)
                     /locus_tag="VIO83_11410"
     CDS             complement(11879..13798)
                     /locus_tag="VIO83_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8331919.1"
                     /translation="MTLLKFTDVSLAYGATPLLDGVSWQIARGERVCIIGRNGTGKSS
                     MLKLVKGDQRPDDGNIWRAPSLKIGELPQELPPADERTVYDVVAEGLAEVGQLIAQYH
                     HLSQHIHSAEDLDKLSHVQQELEARDGWRLQQLVENTLSRLQLPAEKTLAELSGGWRR
                     RVLLAQALVAEPDLLLLDEPTNHLDIGAIAWLEEALLGFNGAVLFITHDRAFLQSLAT
                     RILELDRGNLIDWNGDYASFLVHKEQQLAAEEAANALFDKRLAQEEVWIRQGIKARRT
                     RNEGRVRALKEMRAERSERRERQGTATIQLDGAEKSGKQVIVAEHVSFAHPGGEPLIR
                     DFSMVLQRGDRIGLLGANGTGKTTLLKLLLGELQPTGGSIHIGTKLEVAYFDQLRHQL
                     DAEKTVIDNIAEGREFISIDGQNRHVLSYLGDFLFSPQRARSPVKALSGGERARLLLA
                     KLFSKPANLLVLDEPTNDLDVETLELLEEVLLGFPGTVLMVSHDRAFLDNVVTSTLVF
                     EGEGVVREYVGGYQDWLRQGGSPRLLGVGKEAAGKPAREDKPAAPAPVEPASAAPVAV
                     ARKKLSYKEQRELDALPAQMEALEREQAALQEEVANPDFYLRPQEATHAALARLEFIQ
                     QELDRLLERWAELEG"
     gene            13939..15870
                     /locus_tag="VIO83_11415"
     CDS             13939..15870
                     /locus_tag="VIO83_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY8331920.1"
                     /translation="MRVRPFALFVCLLAGFGLQAQPATAASLPQQRTLYDEAKRALAK
                     NDPAPYLRNRAALRDYPLEPLLAYDELTLRLKSASNAEIEAFLTEHGDLPQIGWMKLR
                     WLRWLAERGDWQTFIKHYSSALNFTELDCLFGEYQFRHGKAADGALTAERLWLVGKSQ
                     PNTCDRLFDLWQARGGLTEELRWKRAKLAAEARNYGLAASLVRTLGSLSPQGRLLLDV
                     AQKPELLLQRQRFAASDRHTADVVGLGLRRLARQNPEQALGLLEEYSRQLPFSNEEKV
                     AIAREIGLTLARRFDARALKVMTQYDPELRDDTVSEWRGRLLLRLGRWAEVNQLTRQM
                     PGDLATTNRWRYWQARSLQLAQPNSQASQSLYQPVAGERDFYGFLAADRINAPYRLNN
                     KPMALDPKVMQKVRNTAGIRRALEFHARGQIVDGRREWYHVSRLFSREELVAQARLGY
                     EMEWYFPAIRAISQAQYWDDLDVRFPMAHQNTLTREARSRGLHSSWVFAITRQESGFM
                     ADARSGVGAMGLMQLMPATAKETARRYGIPLASPQQALNPNVNIQLGAAYLSQVYGQF
                     GGNRILASAAYNAGPGRVRQWLSGNERMPFDIWIETIPYDETRQYVQNVLSYSVIYGE
                     KLGAPQKLVEWHERELGPQ"
     gene            complement(16002..16808)
                     /locus_tag="VIO83_11420"
     CDS             complement(16002..16808)
                     /locus_tag="VIO83_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016852662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY8331921.1"
                     /translation="MLRLSALYRFPVKSLGGESLQRVAVDALGLAGDRRWMVVDSRSG
                     RFLTQRQLPCMAQVGARWLDERTLQLSASGQAALQVPVPPADQALRGVSVWSDSLQAP
                     DAGDAAAQWLSALLGRDCRLVHVPVSRARQVDTAYAQVGDKVGFADGFPLLLIGQASL
                     DDLAARVGRPLEMLRFRPNLVIEGAAPYAEDGWRRLRIGEVEFERVKPCSRCAIPTLD
                     PHSGERADDGEPLRTLATYRRDADGVYFGQNLIQRGTGVLEVGMPVTLLD"
     gene            complement(16878..18893)
                     /gene="uvrB"
                     /locus_tag="VIO83_11425"
     CDS             complement(16878..18893)
                     /gene="uvrB"
                     /locus_tag="VIO83_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY8331922.1"
                     /translation="MSKFHLVTRFKPAGDQPQAIRQMVEGIEAGLSHQTLLGVTGSGK
                     TFSIANVIAKVQRPTLVLAPNKTLAAQLYGEFRSFFPDNAVEYFVSYYDYYQPEAYVP
                     SSDTYIEKDASINDHIEQMRLSATKALLERPDAIIVATVSSIYGLGDPESYLKMVLHV
                     DRGDKLDQRELLRRLAELQYTRNDMDFARATFRVRGDVIDIYPAESELEAIRIELFDD
                     EVESLAAFDPLTGEVIRKLPRFTFFPKSHYVTPRETLLEAVEQIKVELKERLEYLRAH
                     DKLVEAQRLEQRTRFDLEMIIELGYCNGIENYSRYLSGRDPGAPPPTLYDYLPDDALL
                     VIDESHVTVPQVGAMYKGDRSRKETLVEYGFRLPSALDNRPMKFEEWEAASPQTIFVS
                     ATPGPYEAEHAGRVVEQVVRPTGLVDPQIEVRPATTQVDDLLSEIRKCVAAEERVLVT
                     TLTKRMAEDLTDYLSDHDVRVRYLHSDIDTVERVEIIRDLRIGAFDVLVGINLLREGL
                     DMPEVALVAILDADKEGFLRSERSLIQTIGRAARNLSGRAILYADRMTGSMQRAIDET
                     ERRRTKQIAFNDKHGIVPQGVKKDVQDILEGASVPGSRSRKRGAAAKAAEDSARYEAE
                     LRSPSEITKRIRQMEEKMFQLARDLEFEAAAQLRDEIQKLRERLLAL"
     gene            19119..20315
                     /locus_tag="VIO83_11430"
     CDS             19119..20315
                     /locus_tag="VIO83_11430"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HEY8331923.1"
                     /translation="MSLFSAVEMAPRDPILGLNEAFNADTRPGKINLGVGVYFTEEGR
                     IPLLRAVSEAEKARLAAQSPRGYLPIEGIAAYDSAVQKLLFGADSALLAEGRVVTTQA
                     LGGTGALKTGADFLKRLLPNAVVAISNPSWENHRALFESAGFPVQNYSYYDAATQGVN
                     LTGMLADLRALPAQSIIVLHACCHNPTGVDLSSEDWKQVLEVVRERGHVPFLDIAYQG
                     FGDGIAEDAEAVRLFADSGLQFFVSSSFSKSFSLYGERVGALSIVTESREEAGRVLSQ
                     VKRVIRTNYSNPPTHGASIVAAVLNSPELRALWEEELGEMRQRIRSLRTSMVEQLAAH
                     GAKRDFGFVARQRGMFSYSGLTSEQVERLKNEFGIYAVGTGRICVAALNSKNLEVVTR
                     AIVQVL"
     gene            20385..20460
                     /locus_tag="VIO83_11435"
     tRNA            20385..20460
                     /locus_tag="VIO83_11435"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20418..20420,aa:Asn,seq:gtt)
     gene            20765..21016
                     /locus_tag="VIO83_11440"
     CDS             20765..21016
                     /locus_tag="VIO83_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331924.1"
                     /translation="MTIRTLKKSLGHTGEAQDEWEQITDGSIVVSYNTVRANADVSWR
                     PYAKEDDWQDLPYVSADLPGSAEKILEFVNDYLELNEED"
     gene            complement(21033..22058)
                     /locus_tag="VIO83_11445"
     CDS             complement(21033..22058)
                     /locus_tag="VIO83_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY8331925.1"
                     /translation="MKVRFDSPKETRPAEEQGLQVLYAPGKRMAFRLRWYLLLLLVAS
                     PLLWLGGQWLLALERIEAPARLYVPALQVRAFESAQIREILVEPGQRVAKGDLLMRLD
                     NPQWRERLALLADAEAAVPVAAGELPSRERQLLRSAAGAAEQRLGEVRRLLARGAATR
                     VELFQAEAERDARQQALLQFEQRLLQPEPAAQVQRRLERRWLEQRLAGLDVLAPEDGR
                     VLDVAVDPGESVAAGLPLLTLEKRQQVQVWVYLPARHAAYAQPGQELGLQLPDGTWQA
                     ARVVRQVYDTARVPAELQPPFASAARTLLVLVEPTEALADLWRINQLGLQARFPRDWA
                     RTFSLGD"
     gene            complement(22069..>22571)
                     /locus_tag="VIO83_11450"
     CDS             complement(22069..>22571)
                     /locus_tag="VIO83_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331926.1"
                     /translation="RAGLQRLLQALAGLPFAALHLDLEVEQLGWPVPAQRLRDWLDTL
                     ALAARESEWPLELSSHHRWFAEESGEVCVPCALPQLGVGQVSLMIYTANAARSAELAA
                     SIARRWPALRFRLAQSVEPQLPATESLAGQSAGELQRQVGRWQAQLQPEALAGIDWQA
                     WSHYPR"
BASE COUNT         3600 a         7836 c         7419 g         3716 t
ORIGIN      
        1 cggtagccca gctcgccgcg caggatgtcc tgcagccagc gccgcgagaa gccggccggc
       61 tgctcgtcga cctgcgggta gatcacgtgg gcgggcatga tgccgtcgag ctgcggcgcc
      121 agggcgacga agggcttgag gtcgcaggcg cggatctcgt ccagcgagcg ctcgtcgcgc
      181 ggaatcgcca cgtgcgaatc ggcctccggc cagccgtggc cggggaaatg cttgccggtg
      241 gcggccatgc cggctgaccg catgccggca atgaaggccg tcgccagctc gctcacctgc
      301 gccgggtcgc tgccgaaggc gcgcgagccg atcaccgtgc tgcgcccgtg gtcgagatcg
      361 agcaccgggg cgaagctgaa gtcgagaccg accgccagca cctcggtggc catcagccag
      421 ccgcaatggc cggccaggcg cagggcatcc ggcgcggcgg ccagcgcctg catcgacggc
      481 aggcgcacga agccctggcg caggcgctgc acccggccgc cctcctggtc caccgccagc
      541 agcaggtcgg gacgctcggc gcggatcgcc acgcacagct cgcgcacctg ctgcggatgc
      601 tcgatgttgc gggcgaacag gatcagcccg cccacctcgg gctggcgcag cagacgacga
      661 tcctcggccg tcagccaggt gccggcgata tccagcatca gggaaccaga catgaatact
      721 cctcaggcga gcgcggcagc ctgccactgc ggctgcggcg cctcgtcaat gatcacggaa
      781 cagtgcggcg gcacctgctc gaacagcgcc agcaggtcgg cgttgcgcag gcgaatgcag
      841 ccgtgcgagc gcgcctcgcc catcggctcg ctgtccgggg tgccgtgcag atagatgtag
      901 cggcggaagg tgtcgaccgc gccgaggcgg ttgcgccccg gctcgcagcc gctcagccac
      961 aggatgcgcg tgagtatcca gtcgcgctgc ggatgctgtg cggccagctc ggccgaccag
     1021 acctcgccgg tccagcgccg cccgaccagc accgcgcccg cgggcagatc ggcgccgatc
     1081 ttcgcccgca cctggtgacg cccgcgcggg gtgcaccccg agccctgctg ctcgcccggc
     1141 ccgttgaggg cggtggacac cggcaggcgc aggcacagcc ggcccgcggc aaagccgtac
     1201 aggcactggt cggcgatgga aatgtgcagc agatcgagca tggcggcggc ggcagtcata
     1261 ggggccgcta gcttagctga tccacccggg cgcggcaccc acgggttatt tgccgtggtt
     1321 gttcgccttg ccgttgcggt gcgggcgggc cgcgatcaag gctgggtcgg tcagcgcact
     1381 gtcggcgcgc atgccggcgg ccatgaacgg cacgagaaga ctcagcacct gctcggccga
     1441 gacatccacg tcgaagtcgc gggcaacgat ctcgcgcaac ccctcgatgc cggccatgct
     1501 gaagctcgcc gcgccgagca tgaaatgcac ccgccagaac agctccagcg gtggaatgcc
     1561 cggcaccgcc tcttgcagca gctgcaggta gcgacgaaac accttgccgt acaggccggc
     1621 aaggtagttg cgcagcttgc tctggtgctc gcgcacggcc atgccgagca ggcgcatgaa
     1681 cacccggagg ttgctgctgc cccggctgac ctccagcgcc agcgccacca gcaattcgag
     1741 cagctcctcg agcgtcgcgg gcttgccctc gcggctctcc agctcgccct cgaggcgctg
     1801 gcagaacggt ccgaggaaac gcacgaatac cgcctggatc agcgcgtcct tggagccgaa
     1861 gtgataattg accgctgcca ggttgacccc cgccttggcg gtgatcaagc gcagcgaggt
     1921 ctcggtgaaa cccttctcgg caaacaactg ctgcgccgca tcgagaattc gttcaactgt
     1981 tgcagactca accatgacta ctccatcagt cagacatctg ccccaaaccc ggggaacaat
     2041 ctatgtatct gacaagtatg gcaggctccg gccgcagcgc cagaaagccg cagcggccgc
     2101 gaagggtttg cgggggccat aaccctgtat atacttccag caatcagaaa agacagagcc
     2161 cgcccccatg cagaaactga ctccgcgcca gggcgaaatc ctggctttca tcaagacctg
     2221 gctggacgcg cacggttatc cacctacccg tgccgaaatc gcccaggagc tgggtttccg
     2281 ctcgcccaat gcggccgagg agcatctgcg cgcgctggcc cgcaagggcg ccatcgagat
     2341 gattcccggc gcctcgcgtg gcatccgcat tcccgatagc gcgccggcac tgcgcgaagg
     2401 cgaactgccg atcatcggtc gggtcgctgc cggtgcgccc atcctggccg agcagcatat
     2461 cgaggacagc tgccggatca gccccgagtt cttccatccc cgcgccgact tcctgctgcg
     2521 cgtgcacggc atgagcatga aggatgtggg cattctcgac ggcgacctgc tcgccgtgca
     2581 tgcctgccgc gaggcggtca acggacagat cgtggttgcg cggatcggtg atgaagtgac
     2641 ggtcaagcgt ttccagcgcc gcggcaagcg cgtgcagctg ctggcggaaa acccggaatt
     2701 tgccccgatc gtgatcgatc tggagcagcc ggatcaggag ttcgtcatcg aagggctgag
     2761 cgtcggcgtc attcgccgct aaacgccgcc ggcagggtcc gctgcgaatg ctgcgggccg
     2821 cggggaatca acttgaggtg cgggcgcggc gactcagtgc agcacccgcg gctcgtcgct
     2881 gtactcatgc tcgtagtcgc cgtcggccat gctgccggcc atctgcacgc ccagattgaa
     2941 catggccttg gccacctcga tatgctgccc cagcaggaag gccttggcat cctcggaaaa
     3001 ctcgagggtc accaaggcct cctcgtcgtc gccacgacgc agaacgatac ggccatcggg
     3061 caattccacg atttccagga aggaggtcgg catggggagg aagctccgcg aaaaaaggcc
     3121 gacattgtaa cagcgccatg cgtcacgccc tagcgtgacg acggcatccg ctggccacgc
     3181 gtcggacaaa ggcgccgcct gatcgcccgg caccccgccc gcttgccgcg caggcgtcgg
     3241 cgttcaccac tcactcatgc cgtcgcgaaa gcgcgtggcc agcgccttga tctgctgctg
     3301 ccaggcgacg aggctgtcca ggcccagctc gccctcctct gcctggccac ccacatcgac
     3361 cgccgtgatc agcgccagcg ccgggtcgac cttggccttc ttcgcctcgc gcggcggctc
     3421 gaacagctgg cgataggccg ccagcagctg ccccagccag ctctgccggt ccagtgccag
     3481 atccaccagt tcggccagct ccgggctggg cgcagcgtcc agcacctcgc gggtcagcaa
     3541 ctgctcgacc tgcggcgcag ccgcctgggg caagcggtag tagccggcga tctcgtgaca
     3601 cagcccgagc acggcgccat acagatggaa cagcgcgcct tcgcgagcgg cctggatctg
     3661 cgtgcgggca ttcagcgcct gcgccgcgtc ggcggcacgc caggcgtcca gcgccagacc
     3721 ggcgaagtag agtttctggt tggtgcgggt gtacagctcg ttggccatgg cggactcctc
     3781 gacagatgtc caagtatacg ctcgcgcccc ctgcaccggt cgccagcgga agcgggcctg
     3841 cagcaggatc gcgcgccccc aaaagcgaaa ccggccgggc gtctgcacgc accggccggt
     3901 ttggttgcag cgccaggggt aggactcagc gcttgtcttc caccgtccac ttgccgtcgg
     3961 cgtagaacgc gcgccagccg ctgggcttgc cgtccacctc gctctgcacg tactgctcct
     4021 tggtcttgcg gctgaagcgg atcaccgccg ggcggccgtc cgggtcctgc tgcggggcgt
     4081 cgagcaggaa gtggtacttg gcatccagtt cgtccttgtg cggaatcagc tcggctacca
     4141 gcggggcgcg ggtttcacgg ttcttcggga acaggctggc ggcgaggaac aggcccgagg
     4201 cgccgtcacg cagcacgtag acgtcgtcga ccttggcgca gcgcagctcc ggcatcttga
     4261 tcgggtcgat cttcggcggc gccgcctcgc cgctcttcag cagcttgcgg gtgttcttgc
     4321 aggcgctgtt ggtgcagccg aagaacttgc cgaaacggcc ggtcttcagt tgcatctcgc
     4381 tgccgcactt gtcgcactcc aggctcgggc cttcgtagcc cttgatgcgg tactggcctt
     4441 cctcgatctc gtagcccgag cagtccgggt tgttgccgca gatgtgcagc ttgtgctgct
     4501 cgtccagcag gtaggcgtcc atcgccgtgc cgcacttggc gcagcgacgc ttgctgcgca
     4561 gcacgcggga ctccgattcg ccctcgtcgt cggcggcgat ctcgtcgccg gggatcaggt
     4621 tgaccgtggc cttgcagcgc tccttgggcg gcagcgcgta gccggagcag ccgaggaaca
     4681 cgccggtgga ggcggtacgg atggtcatcg gccggccgca ttccttgcac ggaatatcgg
     4741 tcagggtcgg caggttggcg cgcatgccgc tgtcgctggc tgcggccacc tcgagcttgc
     4801 tcttgaagtc gccgtagaac tcgtcgagca ggtgcttcca ctcgcgctcg ccctgagcca
     4861 cgtcgtcgag gttctcttcc atgccggcgg tgaagccgta gtccatcagg ttggcgaagc
     4921 tctccgacag ccgctcggtg acgatgtcgc cgatcttctc ggcgtagaag cggcggttat
     4981 gcagggtcac gtagccgcgc tcctggatgg tcgagatgat cgccgcatag gtcgagggac
     5041 gaccgatgcc gcgcttttcc atctccttga ccaggctggc ttccgagtag cgcgccggcg
     5101 gcttggtgaa gtgctgggcc ggatcgagct ggagcagatc gagctggtcg cgctggagca
     5161 tgtcgggcag cacgtcgtcc tcgcccggct tgctctgcgg cggctgcacg cgggtgaagc
     5221 cgtcgaattt gaggatgcgg cccttggcgc gcagctcgaa atcgccggcc ttgaccgtca
     5281 cgctggtgga caggtactcg gccggcggca tctggcaggc gacgaactgg cgccagatca
     5341 gctcgtacag gcgctcggcg tcgcgctcca tgccggtcag ctgggtcggc ttgaggttga
     5401 cgtcggaagg acggatcgcc tcgtgtgctt cctgggcgcc ttccttgctg ctgtacacgt
     5461 tcggcttggc cggcaggtac tgctggccga actcgccctc gatgaagccg cgcgccatgt
     5521 cgatggcatc ctgcgacagg ttggtcgagt cggtacgcat gtaggtgatg tagccggcct
     5581 cgtacaggcg ctgggccatc atcatggtct tcttcacccc gaagcccaga cggttgctcg
     5641 ccgcctgctg cagggtggag gtgatgaagg gcgcggacgg cttgctcgag gtcggcttgt
     5701 cctcgcgctt ggccacctgg taggcggcgg acttgagcgc ggccacggcg gccatcgcct
     5761 gggcttcgtt gaccggcttg aacgcctcgc cgtgctggcg agccacctcg aagcgcacct
     5821 tggcctgctt ggcagtcgcc aggtcggcat gcacgtccca gtattcttcc gggacgaagg
     5881 cgcggatctc gcgctcgcga tccaccacca gcttcaccgc caccgattgc acgcggccgg
     5941 cggacaggcc gcgggcgatc ttggcccaca gcagcggcga gaccatgtag ccgaccacgc
     6001 ggtcgaggaa acgccgcgcc tgctgggcgt tgacccggtt gatgtccagc tcgccgggct
     6061 gggagaaggc gtcctggatg gccttcttgg tgatttcgtt gaacaccacg cgcttgtagc
     6121 ggctgtcgtc gccgccgatg gcttcgcgca ggtgccaggc gatcgcctcc ccctctctat
     6181 ccaagtcggt tgcgagatag atggtgtcgg catccttggc caggcggcgc agctcctcga
     6241 tcaccttttc cttgccgggc aggatctcgt actgggcctt ccagccgtgc tcgggatcga
     6301 tgcccatgcg cgcgaacagc tggcgcttgg ccttttcctt gggcgtcagc accggcgcat
     6361 cggatgcggc ggccttgccg cgcttgaccg gctccttgct ggccgagccg ctggtgggca
     6421 ggtcgcggat atgaccgatg ctcgacttca ccacgtactg gttgcccaga tacttgttga
     6481 tcgtcttggc cttggccggt gattccacga tgaccagaga tttgcccatg aatcggagag
     6541 ttcctgaaaa gatgtcgaga tgaacggcga ggaccgtcgg cgcaaaaaat gcaattaaat
     6601 gtaactgcgc cctgataaag ccacgctata tatagtggcc cgcacaacga ggtcaagcac
     6661 ggggcggtgc cggatcgtcc tcagcgtagc gaaattccac cctttcgctg cgcaccagag
     6721 caaagcgcgg caacgtctcg ccctccactt ccacggtttc gcgaaacatc gccagcggcc
     6781 gtacccacag gccgtagtcg ccatacagcg cctggtagac caccagctct tcctcggtct
     6841 cggagtgccg ggccagcccc agcacgcgat actcgcggcc cttgtagtga cggtagagcc
     6901 cggtttgcag cgtcatgcgc atttccctca gggtgtcgcc gaaacgagaa ccggggcaca
     6961 caggccccgg ttctcggcca atgggctatc agacgcgctc gaacaccgtc gcgataccct
     7021 gtccgagacc gatacacatg gtggccagac cgagggtcgc gccgcgctgc ttcatcacgt
     7081 tgagcagggt gccggaaatc cgcgccccgg agcagccgaa cgggtggccg agggcaatcg
     7141 cgccgccatt caggttgacc ttgtcgtcca tcttgtccag cagtttgagg tccttgagca
     7201 ccggcagcgc ctgggcggca aaggcctcgt tgagctcgac gcagtcgata tcctccaggc
     7261 tcaggccggc gcgctgcagc gccttgcgcg tcgccggcac cgggccgtag cccatgatcg
     7321 acggatcgac gccggccacc gccatggcgc ggatccgcgc cagcggctgc aggccgagat
     7381 ccatggcgcg ctgcccactc atcacgatca tgcacgaggc gccgtcggtg atctgcgagg
     7441 agctaccggc agtcacggtg ccgtgcttcg gatcgaacgc cggcctgagc gcggccaacc
     7501 cttccacagt ggtttccggg cggatggttt cgtcgtagtc gaagaccttg agcaagccat
     7561 gctcgtcgtg gccttccagg gggatcagct cgtcgcggaa caggccggtc acggtcgcct
     7621 gatgcgccag gcggtgcgag cgacaggcga actggtcctg ggcctcgcgg ctgatgccgt
     7681 gcatcttgcc cagcagttcg gcggtcaggc ccatcatgcc ggcggccttg gccacgtgca
     7741 gcgacagctg cgggttggca tccacgccgt gggtcattgg caggtggccc atgtgctcga
     7801 cgccgccgat cacgaacacc tcgccgttgc cgctctggat cgcttgggcg gcgctgtgca
     7861 gtgcgctcat cgacgagccg cacaggcggc tgacggtctg cgcgccgctg gtatgcggga
     7921 tcggcgtcag cagcgcggcc atccgcgcga tgttccagcc ctgttcgagg gtctggttga
     7981 cgcagcccca gatcacgtcc tccacctcgg ccggatcgac cagcgggttg cgcgccagca
     8041 gcttgctgat cagctgcgcc gacaggttct cggcacgggt gtggcgatgc atgccgccct
     8101 tggaacggcc catcggcgtg cggccgaagt cgacgatcac cacgtctctc ggattcaggc
     8161 tcataagatt ctctagctcc agtggtgatg cttcaggcga agaacgtgcg gccgtcgcgg
     8221 gccatctcgc gcaggccagc ggtcgactgg tacagcggcc ccagcgcgac atggtgatcg
     8281 gccagcgcga cgaacgcggc cacgccgatg gcgtcgatgt agcgcagcgc gccgccccgg
     8341 aagggcggga aaccgatgcc gtagaccagg cccatatccg cctcggcggc gctgtcgacg
     8401 atgccatcct ccaggcagcg caccgtttcc agacacaggg gcagcatcat gatggcgacg
     8461 atgtcctcgt cggacagctc gcgcggcgcc ccgagcaccg gcttgagcag ctcgtgcacg
     8521 ctcgggtcgg cgaccttctt cggcttgccg cccttgtcgg tctggtaggc gtagaagccc
     8581 cgcccgctct tctggccgag gcggcccgcc tcgtacagca cgtcgatggc gctgcgccag
     8641 tcgtcctgca tgcgctcggg atagcccgcc gccatcacct cgcgggcatg gtgggccgtg
     8701 tcgatgccga ccacgtcgag caggtaggcc ggccccatcg gccagccgaa cttctccatc
     8761 acctggtcga tgcgctggaa atcgatgccg gcggcgatca ggcgggcgaa gccgccgaag
     8821 tagggaaaca gcacgcgatt gaccaggaag cccgggcagt cgttgaccac gatcggcgtc
     8881 ttgcccatct tcctggcgta ggccacggtg gtggcgatgg ccgctgcgct ggtctgctcg
     8941 ccgcggatca cctccaccag cggcatcatg tgcaccgggt tgaagaagtg catgccgcag
     9001 aagttttccg gacgctgcag ggccttggcc agcaggttga tcgagatggt cgaggtgttg
     9061 ctggccagaa tggtgtccgc cctgacctgc ccctccacct cggccagcac cgcctgcttg
     9121 accttggggt tttccaccac ggcttccacc accaggtcga cctcggcgaa atcgccgtag
     9181 gacagggtcg gccggatggc attcagcgcc gcggccatct gcgccggcgt caggcggccc
     9241 ttctccacgc gctggccgag cagcttggcc gcttcgccga ggccgagctg gatggcctcc
     9301 tcgcggatgt ccttgagcag gatcggcgtg cccttgactg ccgactggta ggcgatgcca
     9361 ccgcccatga taccggcgcc gagcaccgcg gccagctcca cctcgcgggc ctgcttctcc
     9421 cagccgcgcg ccttgcgctt gagctcctgg tcgttgtgga acaggccgac caggctgcgc
     9481 gccacctcgg tcttggccag cttgaccagc gcctgcgact cgatctccag agccttgtcg
     9541 cggccgtgag cggcggcctt ctggatggtc ttcaccgcct cgagcggtgc cgggtagtgc
     9601 ggtccggcct ggccggccac gtagcccttg gcgctctcga aggccagcat ctgctcgatc
     9661 tggttgagct tgagcttgtc cagcttgggc tggcggcggg ccaggtgatc gagttcgccg
     9721 gcgatggcgc ggcgacacag atcgagggcc gcctcgcgca gtcttgccgg ggcgaccacc
     9781 gcgtcgacca caccgacctt cagcgccgtc tgggcatcgt tttccttgcc ggaggcgatc
     9841 cactcgatgg cgttgtcggc gccaaccagg cgtggcaggc gcacggtgcc gccccagccg
     9901 ggattgatgc ccaacttcac ttccggcaga ccgatcctgg cgccctcggc catcacccgg
     9961 aagtccgccg ccaggcacat ctccaggccg ccgccgaggg cgagaccgtt gatcgccacc
    10021 accgtcggca gcggcagatc ctcgaaggcg ctgaagaggc cgttggtttc caggttggcg
    10081 gccagcaggg cagcctccgg cagggcgaac aggccgagga attcgccgat atcggcgccg
    10141 acgatgaagg cctccttgcc gctggtgacg atcaccccgc ggagcgcggc attgccacgg
    10201 atctccgcaa tggcgctgcc caactcgctc agggtcagcc ggtcgaactt gttgaccgac
    10261 tcctccgcgc ggtcgaaccg caactcggcg atgccgtcct cgagcgcctg aaccgtgatg
    10321 gctttaccct ggtaaatcat cgcctgatct ccatgctatg taagcgcgaa cccgctgcag
    10381 ccggcatgcc ggtgcccaag agcatagccc ctgaccatcc cgcatgatcg gtggcaggga
    10441 cgaccgaacc aggcaagcag ccgactcata tacttgtttt atcgatgccc ggcaccttcg
    10501 gggaagtccg caggcttgtc aattgcgcca aacggcggcg gccacccttg cgcgtcaggc
    10561 atgaccaatg tcatgccgag gcccgtgcgc aggattcacc atctttcgga ccagctgcga
    10621 accgggccat actggccgaa atacccgctg ccaaggagaa cagccatgac ctaccaacat
    10681 ctgctgatcg cggtcgacct gaccgatgac tgccacaagg tgatcgagcg cgccgagtcg
    10741 ctggccggct gctgcgcggc caagctgacc ctggtgcacg tgatcgagcc catgtccatg
    10801 gccttcggcg gcgatgtgcc gctggacctc tcggccctgc aggagcagca gaccgagcag
    10861 gcccggcaac agctggaagc cttttccgcc cagcacccgc aactgggcaa ggaaaactgc
    10921 cgactgctgt tcggccagcc acgccaggaa atccatcgtc tggcccacga actgggctgc
    10981 gacctgatcg tgaccggcag ccacggccgc catggcctgg ccctgctgct cggctcgacc
    11041 tccaacgacc tgctgcacgg cgccacctgc gacgtgctgg cggtgcacct gcccaagccg
    11101 gagtaagtcg agatggcgtg cgccgcatca ggccttgcac ctgacgcggc gccagagcct
    11161 cagagcaggc cgtactcgct gctgctgctc atcaccagcg ggcgcagctt gctgagctgg
    11221 gcttccagcg aataggacac gctggacgcc atggagaaga agctcaggaa ggccttgagg
    11281 ttggagtcgc cctcgcaggt ggcgaggatg cgctgccaga acgcctggtt gaccagctgc
    11341 agctgctggt aattcttgat caagcccttc agctcccagt cggcatgccg gccttcgcgc
    11401 aggttgtagt actggcgcat caggtacagc gagacggtgc gcaggatgaa ctcctggttg
    11461 ctggcgaacg gcaggtgctg ctgggccatc ggcttgagcc ggcagagcat cgggcaggcg
    11521 ctggtggcca tcagcacgcc gagcagcgag cgcagcccgg tctccaggct gacctccttg
    11581 tagtactcgc gctcgggggt gatcacccgc accttggcct tctggaaggc cggcagcccg
    11641 cggaaatcct cgatcacccg gtacaggtca accgccgccg ggcagtggtc gtgcaggtcc
    11701 ttgttcagcg ggcagttgct gcactgctcg tgctccagcc gagtccagcg tggcgcttcg
    11761 ccagcctgca gcgggtcgta gctgcgatcc agttcgaccc ggtagttgaa ctcgtgattg
    11821 tcgtccaggg tgatcttata gtcgatggtc attccctact ccgtgtcctt atcagtcatc
    11881 aaccttccag ctcggcccag cgctccagca aacggtccag ctcctgctgg atgaactcca
    11941 gccgggccag cgccgcatgg gtggcttcct gcggccgcag atagaagtcg gggttggcga
    12001 cttcttcctg cagggccgcc tgctcgcgct ccagtgcctc catctgggcg ggcagcgcat
    12061 ccagctcacg ctgctccttg taactcagtt tcttgcgcgc gactgcgacg ggcgccgcac
    12121 tggccggctc caccggcgcc ggcgccgccg gcttgtcttc gcgtgcgggt ttgcccgcgg
    12181 cttccttgcc aacgcccagc aggcgtggcg agccgccctg gcgcagccag tcctgatagc
    12241 cgccgacgta ttcgcgcacc acaccctcgc cttcgaacac cagcgtgctg gtgaccacgt
    12301 tgtcgaggaa ggcacggtcg tggctgacca tcagcacggt gccagggaag ccgagcagca
    12361 cttcctcgag cagctcgagg gtttccacgt ccaggtcgtt ggtcggttcg tccagcacca
    12421 gcaggttggc cggcttgctg aacagcttgg ccagcagcag acgcgcacgc tcgccaccgg
    12481 acagcgcctt gaccggcgag cgcgcacgct gcgggctgaa caggaagtcg ccgagatagc
    12541 tgagcacgtg gcggttctgc ccgtcgatgc tgatgaattc gcggccttcg gcgatgttgt
    12601 cgatcaccgt cttttccgcg tcgagctggt ggcgcagctg gtcgaagtag gccacttcca
    12661 gcttggtgcc gatatggatg ctgccgccgg tcggctgcag ttcgccgagc agcagcttga
    12721 gcagggtggt cttgccggtg ccgttggcgc cgagcaggcc gatgcggtcg ccgcgctgca
    12781 ggaccatgga gaagtcgcgg atcagcggct cgccgcccgg gtgggcgaaa ctcacgtgct
    12841 cggcgacgat cacctgcttg ccggactttt ccgcgccgtc cagctggatg gtggcggtgc
    12901 cctgacgctc gcgccgttcg ctgcgctcgg cacgcatttc cttgagcgct cgcacgcggc
    12961 cttcgttacg ggtgcgacgg gccttgatgc cctggcggat ccacacttcc tcctgggcca
    13021 ggcgcttgtc gaacagcgcg ttggccgcct cctccgccgc cagctgctgc tccttgtgga
    13081 ccaggaagct ggcgtagtcg ccgttccagt cgatcaggtt gccgcggtcc agctcgagga
    13141 tgcgcgtggc caggctctgc aggaaggccc ggtcgtgggt gatgaacagc acagcaccgt
    13201 tgaagccgag caacgcctct tccagccagg cgatggcgcc gatgtcgagg tggttggtcg
    13261 gctcgtcgag cagcagcagg tccggttcgg cgaccagcgc ctgggccagc agcacgcgcc
    13321 ggcgccagcc gccggagagt tcggccaggg tcttttccgc cggcagctgc aggcggctca
    13381 gggtattttc caccaactgc tgcaggcgcc agccgtcgcg cgcttccagc tcctgctgca
    13441 catgcgacag cttgtccagg tcctcggcac tgtggatgtg ctgggacagg tggtggtact
    13501 gggcgatcag ctggccgacc tcggccaggc cctcggccac cacgtcgtac accgtgcgct
    13561 cgtcggccgg cggcagctcc tgcggcagct cgccgatctt cagcgacggc gcgcgccaga
    13621 tattgccgtc gtccggacgc tggtcgccct tcaccagctt gagcatgctc gacttgccgg
    13681 tgccgttgcg gccgatgatg cacacccgct cgccgcgggc gatctgccac gagaccccgt
    13741 ccagcaacgg cgtggcgccg taggcaaggg atacgtcggt gaacttgagc agggtcatgg
    13801 gggcgcctcc atcggaatgg gcgcgcattc tactcgcagt tgccagcggc cggggacaat
    13861 gctttgcttt aaccccgcgc ggcagaaggc taagcttgac gccatcgccc cgcccgtccc
    13921 catagcgtcg aaacttccat gcgcgttcgc ccgtttgccc tgtttgtctg cctgctggcc
    13981 ggtttcggcc tgcaggccca acccgccacc gccgcgtccc tgccccagca gcggaccctc
    14041 tacgacgaag ccaagcgcgc cctggccaag aacgaccccg cgccctacct gcgcaaccgc
    14101 gcggcgctgc gcgactatcc gctggagccg ctgctggcct acgacgagct gaccctgcgc
    14161 ctgaagtcgg ccagcaatgc ggagatcgag gccttcctca ccgagcacgg cgacctgcca
    14221 cagatcggct ggatgaagct gcgctggctg cgctggctgg ccgagcgcgg cgactggcag
    14281 accttcatca agcactactc ctcggccctc aacttcaccg agctggattg cctgttcggc
    14341 gagtaccagt tccgccacgg caaggcggcc gacggcgcgc tgaccgccga gcgcctgtgg
    14401 ctggtgggca agtcgcaacc caacacctgc gaccgcctgt tcgacctctg gcaggcccgc
    14461 ggcgggctga ccgaggagct gcgctggaag cgcgccaagc tggccgccga agcgcgcaac
    14521 tacggcctgg ccgccagcct ggtgcgcacc ctcggcagcc tgtcgcccca gggccggttg
    14581 ctgctcgacg tggcgcagaa acccgaactg ctgctgcagc gccagcgctt cgccgccagc
    14641 gaccgtcaca ccgccgacgt cgtcggcctc ggcttgcgcc gcctggcccg gcagaatccc
    14701 gagcaggccc ttggtctgct cgaggagtac agccgccagc tgcccttctc caacgaggaa
    14761 aaggtcgcca tcgcccgcga gatcggcctg accctggcgc gccgcttcga tgcccgcgcg
    14821 ctcaaggtga tgacccagta cgaccccgag ctgcgcgacg acaccgtcag cgagtggcgc
    14881 ggccgcctgc tgctgcgcct ggggcgctgg gcggaggtca accagctgac ccggcagatg
    14941 cccggcgatc tggccaccac caaccgctgg cgctactggc aggcgcgcag cctgcagctg
    15001 gcccagccca acagccaggc ctcgcagagc ctgtaccagc cggtggccgg cgagcgcgac
    15061 ttctacggct tcctcgccgc cgaccggatc aacgcgccgt accggctcaa caacaagccg
    15121 atggccctcg atccgaaggt gatgcagaag gtgcgcaata ccgccggcat ccgccgcgcc
    15181 ctggaattcc atgcccgcgg ccagatcgtc gacggccgcc gcgagtggta ccacgtcagc
    15241 cgcctgttca gccgcgagga gctggtggcc caggcgcgcc tcggctacga gatggagtgg
    15301 tacttcccgg cgatccgcgc catcagccag gcgcagtact gggacgacct ggacgtgcgc
    15361 ttcccgatgg cccaccagaa caccctaacc cgcgaggcac gcagccgtgg cctgcattcc
    15421 agctgggtgt tcgccatcac ccgccaggaa agcggcttca tggccgacgc gcgctcgggc
    15481 gtcggcgcca tgggcctgat gcagctgatg ccggccaccg ccaaggagac cgcgcggcgc
    15541 tacggcattc cgctggcctc gccgcagcag gcgctcaacc ccaacgtcaa catccagctc
    15601 ggcgccgcct acctcagcca ggtctacggc cagttcggcg gcaaccgcat cctcgcctcg
    15661 gccgcctaca acgccggccc cggccgcgtg cgccagtggc tgagcggtaa cgaacgcatg
    15721 ccgttcgaca tctggatcga gaccatcccc tacgacgaga cccgtcagta cgtgcagaac
    15781 gtgctgtcct actcggtgat ctacggggaa aagctcggcg cgccgcagaa actggtggag
    15841 tggcacgagc gcgagctggg gccgcagtaa gtcgaactcg gcgcaaatgt agggtgggtt
    15901 agccgcaccg cggcgtaacc caccaaggag gccggtaggc atcccttcac agcatggcga
    15961 ccaccagggg atggtgtgca cggcgcaccc tgcgaatgcc gtcagtcgag cagggtcacc
    16021 ggcatgccca cctccagcac gccggtgccg cgctggatca ggttctggcc gaagtacacg
    16081 ccatcggcat cgcggcggta ggtcgccagg gtgcgcagcg gttcaccgtc gtccgcacgc
    16141 tcgccgctgt gcggatcgag ggtgggaatg gcgcagcgcg aacagggctt gacccgctcg
    16201 aactccacct cgccgatgcg caggcgccgc cagccgtcct cggcataggg ggccgcgccc
    16261 tcgatcacca gattcggacg aaagcgcagc atctccagcg gccggccgac ccgcgccgcc
    16321 aggtcgtcca gcgacgcctg gccgatcagc agcaacggaa aaccgtcggc gaaacccacc
    16381 ttgtccccga cctgcgcgta ggccgtgtcc acctggcgcg cccggctgac cggcacatgc
    16441 accagccggc agtcgcgacc gagcaacgcg ctcagccact gtgccgcggc atcaccggca
    16501 tccggcgcct gcaggctgtc gctccagacg ctgaccccgc gcaacgcctg gtccgccggc
    16561 ggcaccggca cctgcagggc ggcctggccg gacgcgctca gctgcagtgt ccgctcatct
    16621 agccagcgcg cgccgacctg cgccatgcac ggcagctggc gctgggtgag gaagcgaccg
    16681 ctgcggctgt cgaccaccat ccaacgtcgg tcgccggcca ggccgagggc gtccaccgct
    16741 acgcgctgca ggctttcgcc gccgagggat ttgaccggaa aacggtagag cgcggagagt
    16801 cgcagcatgg ccgggcgtcc tgagggaaaa cgcccttata acggcccgcg cgcaacagcg
    16861 gcaagcgccc gtcggcgcta caacgccagc aaccgctcgc gcagcttctg gatctcgtcg
    16921 cgcagctgcg cggcggcctc gaactccagg tcgcgggcca gctggaacat cttctcctcc
    16981 atctgacgga tgcgcttggt gatctcgctc ggcgagcgca gctcggcttc gtaacgcgcg
    17041 ctgtcctcgg ccgccttggc cgccgcgccg cgcttgcggc tgcgcgagcc gggcacgctg
    17101 gcgccctcga ggatgtcctg gacgtccttc ttcacgccct ggggcacgat gccgtgcttg
    17161 tcgttgaagg cgatctgctt ggtgcgacgc cgctcggttt cgtcgatggc acgctgcatc
    17221 gaaccggtca tgcggtcggc gtagaggatc gcccggccgc taaggttgcg cgcggcgcgg
    17281 ccgatggtct ggatcagcga gcgctcgctg cgcaggaagc cttccttgtc ggcgtcgagg
    17341 atcgccacca gcgccacctc gggcatgtcc aggccctcgc gcagcaggtt gatgccgacc
    17401 agcacgtcga aggcgccgat gcgcaggtcg cggatgatct ccacccgctc cacggtgtcg
    17461 atgtccgagt gcaggtagcg cacccgcacg tcgtggtcgc tcaggtagtc ggtgaggtcc
    17521 tcggccatgc gcttggtcag ggtggtgacc agcacgcgct cctcggcggc cacgcacttg
    17581 cggatttccg agagcaggtc gtcgacctgg gtggtcgccg gacgcacctc gatctgcggg
    17641 tcgaccaggc cggtcgggcg caccacctgc tcgaccaccc gcccggcgtg ctcggcctcg
    17701 tagggcccgg gggtggcgga gacgaagatg gtctgcgggc tggccgcctc ccactcctcg
    17761 aacttcatcg gccggttgtc cagcgccgag ggcaggcgga agccgtactc caccagggtt
    17821 tccttgcgcg agcggtcgcc cttgtacatg gcgccgacct gcggcacggt gacatgcgac
    17881 tcgtcgatca ccagcagcgc gtcgtccggc aggtagtcgt agagggtcgg cggcggcgcg
    17941 ccgggatcgc gcccggacag gtagcgcgag tagttttcga tgccgttgca gtagcccagc
    18001 tcgatgatca tctccaggtc gaagcgggtg cgctgctcca ggcgctgcgc ctcgaccagc
    18061 ttgtcgtggg cgcgcaggta ttccaggcgt tccttcagct cgaccttgat ctgctccacc
    18121 gcctccagca gcgtctcgcg cggggtgacg tagtggctct tggggaagaa ggtgaagcgc
    18181 ggcagcttgc ggatcacctc gccggtcagc ggatcgaagg cggccaggct ctccacctcg
    18241 tcgtcgaaca gctcgatgcg gatcgcctcc agctcggatt cggccgggta gatgtcgatc
    18301 acgtcgccgc gcacgcggaa ggtggcgcgg gcgaagtcca tgtcgttgcg ggtgtactgc
    18361 agctcggcca ggcggcgcag cagctcgcgc tggtcgagtt tgtcgccgcg gtcgacgtgc
    18421 aggaccatct tcaggtacga ctcgggatcg cccagaccgt agatcgacga cacggtggcg
    18481 acgatgatcg cgtccggccg ctccagcagc gccttggtcg ccgacaggcg catctgctcg
    18541 atgtggtcgt tgatcgaggc gtccttctcg atgtaggtat ccgaggacgg cacgtaggct
    18601 tcgggctggt agtagtcgta gtaggagacg aaatactcca ccgcgttgtc ggggaagaag
    18661 ctgcggaact cgccatacag ctgcgcggcc agggtcttgt tcggcgccag caccagggtc
    18721 gggcgctgca ccttggcgat cacgttggcg atgctgaaag tcttgcccga gccggtcacg
    18781 ccgagcaggg tctggtgcga cagccccgcc tcgatcccct cgaccatctg gcggatcgcc
    18841 tgcggctggt cgccggcggg cttgaagcgg gtgacgagat gaaacttgga cataggcgta
    18901 ccctgagagg acaaactcct gcggcaacga gtacgccagg caggcgcccc gaccagaatg
    18961 cgacatcatg tcgcaccagt ggccattttc gccgcacctg cgtatcgccg caccgggatg
    19021 gtgctgtcta taatatcgcc ccgtttgcgt aaccccctag ccgcaatgac ggcagggtgt
    19081 tgcatcgcct cccaccctca ttctccgagc tgccgcacat gagtctgttc tccgccgtcg
    19141 aaatggctcc ccgcgacccc atcctgggcc tcaacgaagc cttcaacgcc gacacccgcc
    19201 cgggcaagat caatctgggc gtaggcgtgt acttcaccga ggaaggccgt attccgctgc
    19261 tgcgcgccgt gagcgaggcc gagaaggccc gcctggcggc ccagtcgccg cgcggctacc
    19321 tgccgatcga aggcatcgcc gcctacgaca gcgccgtgca gaagctgctg ttcggtgccg
    19381 actccgccct gctggccgaa ggccgtgtgg tcaccaccca ggccctcggc ggcaccggcg
    19441 ccctgaagac cggtgccgac ttcctcaagc gcctgctgcc caatgccgtg gtcgccatca
    19501 gcaacccgag ctgggaaaac caccgcgcgc tgttcgagtc tgccggcttc ccggtgcaga
    19561 actacagcta ctacgacgcc gccacccagg gcgtcaacct gaccggcatg ctcgccgacc
    19621 tgcgcgccct gccggcgcag tcgatcatcg tactgcacgc ctgctgccac aacccgaccg
    19681 gcgtcgacct gtcctccgag gactggaagc aggtgctgga agtggtgcgc gagcgcggtc
    19741 acgtgccgtt cctcgacatc gcctaccagg gcttcggcga cggcatcgcc gaggatgccg
    19801 aggcggtacg cctgttcgcc gactccggcc tgcagttctt cgtctccagc tccttctcca
    19861 agtcgttctc gctgtacggc gagcgcgtcg gtgcgctgtc gatcgtcacc gaatccaggg
    19921 aagaagccgg ccgcgtgctg tcgcaggtca agcgcgtgat ccgcaccaac tactccaacc
    19981 cgccgaccca cggtgccagc atcgtcgctg cggtgctcaa cagccccgag ctgcgtgccc
    20041 tgtgggaaga ggaactgggc gagatgcgcc agcgcatccg cagcctgcgc accagcatgg
    20101 tcgagcagct ggccgcccac ggcgccaagc gcgacttcgg cttcgtcgcc cgccagcgcg
    20161 gcatgttctc ctactccggc ttgaccagcg agcaggtcga gcgcctgaag aacgagttcg
    20221 gcatctacgc cgtcggcacc ggccgcatct gcgtcgccgc gctgaacagc aagaacctcg
    20281 aggtggtcac ccgcgccatc gtccaagtgc tgtgaaccga atgcgtgaag tcagcctcag
    20341 ggggttgact tcacctttca aatcagtagg ataggcaccg ttgttccgcg atagctcagt
    20401 cggtagagca aatgactgtt aatcattggg tccctggttc gagtccaggt cgcggagcca
    20461 gattacaaag cctcgggtga aagcccgggg cttttttatt gcccgccgga ctctcgccag
    20521 ggactgcgtc ccaggttatt cggccctaca ggcctccccc ttcggggccg cgctgaagcg
    20581 cgttcggctt gcgccgtcga gtccaggtcg cggagccaga ttccaaaaac cccagcctcg
    20641 gctggggttt tttattgcct gaaaatcgcc cccgccgagc ctcaggcctg cgccgccgag
    20701 aatctcgcct atggttgtag ctaccgaccg ccatgcggcg agccgttatc cggagggggg
    20761 gaacatgacc attcgcacac tgaagaaatc cctgggccac acgggcgagg cgcaggacga
    20821 gtgggaacag atcaccgatg gcagcatcgt ggtcagctac aacaccgtcc gcgccaacgc
    20881 cgatgtcagc tggcgcccct atgccaagga ggacgactgg caggatctgc cctatgtgtc
    20941 cgccgacctg cctggcagcg ccgagaagat cctcgagttc gtgaacgact acctcgagct
    21001 caacgaggaa gactgactgc ccgcgcggcc gctcaatcgc ccagcgagaa ggtccgtgcc
    21061 cagtcgcgag ggaaacgcgc ctgcagcccc aactggttga tccgccacag gtcggcgagg
    21121 gcttccgtcg gctccaccag taccagcagg gtgcgcgccg ccgaggcgaa cggcggctgc
    21181 agctccgccg gcacccgcgc cgtgtcgtag acctggcgca ccacccgggc cgcctgccag
    21241 gtgccatcgg gaagctgcag gcccagctcc tgccccggct gcgcataggc tgcatggcgg
    21301 gccggcaggt agacccagac ctgtacctgc tggcgcttct ccagggtcag cagcggcagc
    21361 ccggcggcga cgctctcgcc gggatcgacc gcgacatcca gcacccgccc gtcttccggc
    21421 gccagcacgt ccagaccggc caggcgctgc tccagccagc gccgctccag ccgccgctgc
    21481 acctgcgccg ccggctccgg ctgcagcagc cgctgctcga actgcagcaa ggcctgctgg
    21541 cgtgcatcgc gctcggcctc ggcctggaac agctcgaccc gcgtcgccgc accccgggcg
    21601 agcaggcgac gcacctcgcc cagacgctgc tcggccgccc ctgcggcact gcgcagcaac
    21661 tggcgctcgc gcgacggcaa ctcgcctgca gccaccggca cggccgcctc ggcatcggcc
    21721 agcagtgcca gccgttcacg ccactgcgga ttgtccaggc gcatcagcag atcgcccttg
    21781 gccacccgct gtccgggctc gaccaggatc tcgcggatct gcgcggactc gaaggcccgc
    21841 acctgcaggg ccggcacgta cagccgcgcc ggcgcctcga tgcgctccag cgccagcagc
    21901 cactgcccgc ccagccacag cagcggactg gcgaccagca gcagcaacag gtaccagcgc
    21961 agacggaagg ccatgcgctt acccggggcg tagagcacct gcagcccctg ttcctcggcc
    22021 gggcgggttt ccttgggact gtcgaaacgg accttcatgg gcgggtgctc aacggggata
    22081 gtgggaccag gcctgccagt cgatgccggc aagcgcttcg ggttgcagtt gcgcctgcca
    22141 gcggccgacc tgccgctgca gctcgccggc gctctgcccg gccaggcttt ccgtcgccgg
    22201 cagctgcggc tcgacgctct gcgccaggcg aaaacgcagc gccggccagc gccgggcgat
    22261 cgacgccgcc agctcggcgc tgcgcgccgc gttagcggtg tagatcatca ggctgacctg
    22321 gcccacgccc agctgcggca gggcgcaggg cacgcagacc tcgccgctct cctcggcaaa
    22381 ccagcggtga tggctggaca gctccagcgg ccattcgctc tcgcgagcgg ccagcgccag
    22441 ggtgtccagc cagtcgcgca ggcgctgcgc cggtaccggc cagcccagct gctcgacctc
    22501 caggtcgagg tgcagggcgg cgaacggcag cccggccagc gcctgcaaca aacgctgcag
    22561 cccggcgcgg c
//
LOCUS       DATCUN010000293        14183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_202698, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000293 DATCUN010000000
VERSION     DATCUN010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 14183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14183
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202698"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..2900
                     /gene="gyrA"
                     /locus_tag="VIO83_11455"
     CDS             123..2900
                     /gene="gyrA"
                     /locus_tag="VIO83_11455"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY8331927.1"
                     /translation="MGELAKEILPVNIEDELKQSYLDYAMSVIVGRALPDARDGLKPV
                     HRRVLFAMSELGNDWNKPYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSVDGDSAAAMRYTEVRMARLAHELLADLDKETVDWVPNYDGTEQIPAVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLGEVIDGCLALIDNADISIDELMRYIPGPDFP
                     TAGIINGRAGIIEAYRTGRGRVVMRARVEVEDIDKVGGRQQLVVTELPYQLNKARLIE
                     KIAELVKEKKLEGISELRDESDKDGMRVVIELRRGEVPEVVLNNLYAQTQMQSVFGIN
                     VVALVDGQPRTLNLKDMLEVFVRHRREVVTRRTVYELRKARERGHILEGQAVALSNID
                     PVIELIKQSPTPAEAKERLIATAWESSAVEAMVERAGADSCRPDDLDPQYGLREGKYY
                     LSPEQAQAILELRLHRLTGLEHEKLLAEYQEILTQIGELIRILSNPVRLMEVIREELE
                     KVKADFGDARRTEIVASQVDLTIADLITEEERVVTISHGGYAKSQPLAAYQAQRRGGR
                     GKSASGVKDEDYIEHLLVANSHATLLLFSSKGKVYWLRTFEIPEASRTARGRPLVNLL
                     PLDEGERITAMLQVDLEALRAQAPEGDEELDDSEGLVIEGELVEAESAEDSDAEVADD
                     EQDEPTGAYIFMATAFGTVKKTPLVQFSKPRSSGLIALKLEEGDTLIAAAITDGAKEV
                     MLFSDGGKVIRFKEKHVRTMGRTARGVRGMRLGEGQQLISMLIPEAQAQILTASERGY
                     GKRTAIEEFPQRGRGGQGVIAMVSNERNGKLVGAIQVQDGEEIMLISDQGTLVRTRVD
                     EVSSLSRNTQGVTLIKLASDETLVGLERVQEPSDNGEDEALDDEHADSAVDTGAEVAA
                     ADESAPQAGEE"
     gene            2991..4076
                     /gene="serC"
                     /locus_tag="VIO83_11460"
     CDS             2991..4076
                     /gene="serC"
                     /locus_tag="VIO83_11460"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEY8331928.1"
                     /translation="MSMRAHNFCAGPAALPEAVLHKAQAELLDWHGKGLSIMEMSHRS
                     DDYVAVAEKAEQDLRDLLAIPSNYKVLFLQGGASQQFAQIPLNLLPEDGIADYVDTGI
                     WSKKALEEARRFGNVNVAASAKAYDYFAIPGQNDWQLSKNAAYLHYASNETIGGLQFD
                     WVPDLGDVPLVVDMSSDILSRPIDVSQFGLIYAGAQKNIGPSGLVVVIVREDLLGKAR
                     SICPTMLDYKVAADNGSMYNTPATFSWYLSGLVFEWLKEQGGVEEMERRNRIKQELLY
                     GAIDASEFYTNPIAKNARSWMNVPFRLADEKLDKAFLAGAEARGLLNLKGHRSVGGMR
                     ASIYNAVGLDAVQALVGYMAEFEKEHG"
     gene            4076..5173
                     /gene="pheA"
                     /locus_tag="VIO83_11465"
     CDS             4076..5173
                     /gene="pheA"
                     /locus_tag="VIO83_11465"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY8331929.1"
                     /translation="MSEKEQLQALRVRIDSLDEQILGLISERARCAEEVARVKMAALQ
                     PGEKPVFYRPEREAWVLKHIMELNQGPLGNEEVARLFREIMSSCLALEHPLKVAYLGP
                     EGTFTQAAALKHFGNAVISQPMAAIDEVFREVAAGAVNFGVVPVENSTEGAVNHTLDS
                     FLEHDLVICGEVELRIHHHLLVGENTKTDKISRIYSHAQSLAQCRKWLDAHYPNVERV
                     AVSSNADAAKRVKSEWNSAAIAGDMAAQLYGLTKLAEKIEDRPDNSTRFLIIGNQEVP
                     PTGDDKTSIIVSTNNKPGALHDLLVPFQARGIDLTRIETRPSRSGKWTYVFFIDFVGH
                     QQDPQVKDVLEQISQESVAVKVLGSYPNAVL"
     gene            5240..6352
                     /gene="hisC"
                     /locus_tag="VIO83_11470"
     CDS             5240..6352
                     /gene="hisC"
                     /locus_tag="VIO83_11470"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY8331930.1"
                     /translation="MSCDFLALAVPGVQKLSPYVPGKPVDELARELSLDPAGIVKLAS
                     NENPLGPSPKALEAIRAELGDITRYPDGSGYQLKAKLAARFGLSPAQITLGNGSNDIL
                     DLVARAWLAPGLNAVFSQYAFAVYPISTQAVGAEGRAVPAKDYGHDLEAMLAAIDANT
                     RVVFIANPNNPTGTWFGPDALESFLSRVPAHVLVVLDEAYIEFAEGDELPNGLEYLAR
                     YPNLLVSRTLCKAYGLAGLRVGYSASSPQVADVLNRVRQPFNVNSLALVAACAALDDA
                     DYLAAGRRNNVAGMAQLEAGFRALGLKWIPSKGNFIAVDFGRDAAPINQALLQEGVIV
                     RPVAGYGMPTFLRVSIGLPAENQRFLEALAKVLGRG"
     gene            6345..8576
                     /locus_tag="VIO83_11475"
     CDS             6345..8576
                     /locus_tag="VIO83_11475"
                     /EC_number="1.3.1.12"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional prephenate
                     dehydrogenase/3-phosphoshikimate
                     1-carboxyvinyltransferase"
                     /protein_id="HEY8331931.1"
                     /translation="MAEPGRAPLVGRLVVVGLGLIGGSFAKGLRQQGLCREVVGVDLD
                     AESRRLAVQLGVVDRCESELATACVGADVIMLAVPILAMEKVLAQLAALDLGDAVLTD
                     VGSAKGNVVRAARQVLGAHIARFVPGHPIAGSEQSGVEAANGALYRRHKVILTPLAET
                     DAQALALVDRLWRALGADVEHMAVEHHDEVLAATSHLPHLLAFGLVDSLAHQSENLEI
                     FRYAAGGFRDFTRIAGSDPVMWHDIFLANREAVLRILDRYRSDLDGLREAIDHGDGHH
                     LLGVFTRARVAREHFSKILAQRAYVDAMHSNDLIFLAQPGGSLSGRIRVPGDKSISHR
                     SIMLGSLAEGTTEVEGFLEGEDALATIQAFRDMGVVIEGPHHGRVTVHGVGLHGLRAP
                     AGPLYMGNSGTSMRLLSGLLAAQPFDTVLTGDPSLSKRPMNRVAKPLREMGAVIETGP
                     EGRPPLNIKGGQRLTGMHYQMPMASAQVKSCLLLAGLYAAGETAVTEPAPTRDHTERM
                     LRGFGYPVEVHGATAKVESGHKLSATHIEVPADISSAAFFLVAASIAENSELVLEHVG
                     INPTRTGVIDILKLMGGDITLENQRIVGGEPVADLRVRSAKLKGIEIPEDLVPLAIDE
                     FPVLFVAAACAEGRTVLRGAEELRVKESDRIQVMADGLQACGVKAEPTPDGIVIDGGP
                     IGGGEVWAHGDHRIAMSFSVASLRASAPIRIHDCANVATSFPNFLALCAEVGVRVAKE
                     EQQ"
     gene            8573..9265
                     /gene="cmk"
                     /locus_tag="VIO83_11480"
     CDS             8573..9265
                     /gene="cmk"
                     /locus_tag="VIO83_11480"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY8331932.1"
                     /translation="MTRLAPVVTIDGPSGSGKGTVCSRLARQLGWHLLDSGALYRLLA
                     LAAGRHGIALDNETALETLAANLDVQFIAAQEGLHGQRILLEGEEVGDELRTEQAGAG
                     ASQVAVLPGVRSALLQRQRDFRVDPGLVADGRDMGTVVFADAPLKIFLTASAEERARR
                     RYLQLKDKVSGVNLTSLLEEIRARDERDMQRSVAPLKPASDAILLDSTELSIEQVLER
                     ILAELALRDLVG"
     gene            9387..11063
                     /gene="rpsA"
                     /locus_tag="VIO83_11485"
     CDS             9387..11063
                     /gene="rpsA"
                     /locus_tag="VIO83_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY8331933.1"
                     /translation="MSESFAELFEESLKSLDMQPGAIITGIVVDIDGDWVTVHAGLKS
                     EGVIPVEQFYNEQGELTIKVGDEVHVALDAVEDGFGETKLSREKAKRAESWIVLEAAF
                     AAEEVVKGIINGKVKGGFTVDVNGIRAFLPGSLVDVRPVRDTTHLENKELEFKVIKLD
                     QKRNNVVVSRRSVLEAENSAEREALLESLQEGQQVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRIKHPSEIVNVGDEIDVKVLKFDRERNRVSLGLKQLGEDPWVAIKARYPE
                     GTRVQARVTNLTDYGCFAELEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDEVEVMVLD
                     IDEERRRISLGIKQCKPNPWEEFSGQFNKGDRISGTIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNEAGEEAVRRFKKGDELDTVILSVDPERERISLGIKQLEDDPFSNYAALNEK
                     GTIVRGTVKEVDAKGAIITLADEVEGVLKASEISRDRVEDARNVLKEGEEVEAKIISV
                     DRKSRVISLSIKSKDVAEEKEAMKELRKQDVSVGPTTIGDLIKAQMGNQN"
     gene            11222..11557
                     /locus_tag="VIO83_11490"
     CDS             11222..11557
                     /locus_tag="VIO83_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2288 domain-containing protein"
                     /protein_id="HEY8331934.1"
                     /translation="MSDAISAGSEPSVLYAKLLGETATITWQELQRFFAGGNVLRVAG
                     ELDLVAVAEALAEDDGAKVQLWLTAGSLRKMEVADAEDYQARDPQLWAVVVAPWVLVQ
                     ERAEAPSLH"
     gene            11629..11994
                     /locus_tag="VIO83_11495"
     CDS             11629..11994
                     /locus_tag="VIO83_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5064 family protein"
                     /protein_id="HEY8331935.1"
                     /translation="MFEPGHIHVANPVAALGLPTYAADLYYEVRTGAKEGPVVHFRMA
                     GEIAGKPFEEEFEMSHDTVFNFASTMGGVAAKHGMPINHSPVLRHHKEFDAIFKDLRA
                     KLKVAVNGPLSAHDPNAGT"
     gene            complement(12014..12781)
                     /gene="phnE"
                     /locus_tag="VIO83_11500"
     CDS             complement(12014..12781)
                     /gene="phnE"
                     /locus_tag="VIO83_11500"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEY8331936.1"
                     /translation="MKRALNYALLLAILLAVAAAFAWLGLDFSGLTSAKGLALMGDYA
                     AGFFPADFSAAHLASIGRASLETLAMSALGTLLAALLGLLLALPAAGRCGRAAAALAR
                     FVLNALRSIPELVWAALMVLAAGLGPNAGTLALALHTTGVLGRLFAEALENTPAEPAE
                     AIRLAGGGHVAAFVYGTLPGVWPQLIAYSLYRWENNIRMASVLGFVGAGGLGQMLYVS
                     LSLFQQAQAATVILAMLLLVLAVDALSGWARQRWVSH"
     gene            complement(12778..13635)
                     /locus_tag="VIO83_11505"
     CDS             complement(12778..13635)
                     /locus_tag="VIO83_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8331937.1"
                     /translation="MLSHSTAPAKTAAEDLRDPAALPRLLLTLLALALLWPGLQLSEL
                     NLAVLFDPGNAATMGSFLAAFWPPAQDGEFLALLGRATLETLAIATAGMALAWLLAVP
                     CALLATRVLSVSALARGGRPRWWANALRWPVRLLLIVLRSIPEIVWALLFVRAVGLGP
                     TAGVLAIAITYSGMLGKVYAEICESVDPRPARALLAAGSPRLTAFAYGVLPNAAAEML
                     SYTVYRWECAIRASVVMGFVGAGGLGQQIDLSMRMFAGGEVASMLLTFFALVLLADQL
                     SRLLRSRLA"
     gene            complement(13629..>14183)
                     /locus_tag="VIO83_11510"
     CDS             complement(13629..>14183)
                     /locus_tag="VIO83_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8331938.1"
                     /translation="IHQSPPLPPRQRVVTAVLAGRLGQWPLWKGLLNLLHPLDSTGAR
                     DALARLDLADKLFERCDQLSGGQLQRVGIARVLYQQAELLLADEPVSAMDPVLASHTL
                     GVLTREAESRGVTLLASLHAVELALAHFPRIVGVRDGRIAFDLPAHEVTPQALDALYA
                     NEQLDLPLPASPMPAPQLVTIPRC"
BASE COUNT         2521 a         4768 c         4763 g         2131 t
ORIGIN      
        1 ccgcgccgca ggccatccgg catgcttctt cgttacaccg ccggcctcca gcgactcccc
       61 gccttgtgtt accattccgc gcttatcgca ggaccctgct gactaaaagg aaccaggctt
      121 ccatgggcga actggccaaa gaaatcctcc cggtcaatat cgaagacgaa ctcaagcagt
      181 cctacctcga ttacgccatg agcgtcatcg tcggacgggc gctgccggat gcgcgcgatg
      241 gtttgaagcc ggtgcatcgt cgcgtgcttt ttgccatgag cgagctgggc aacgactgga
      301 ataagccgta caagaagtct gcccgtgtgg tcggcgacgt gatcggtaaa tatcacccgc
      361 acggcgattc agccgtctac gacaccatcg tgcgtatggc ccagccgttc tcgctgcgct
      421 acatgctggt cgatggccag ggcaacttcg gctccgtcga cggtgactcc gccgccgcca
      481 tgcgttacac cgaggtgcgc atggcgcgcc tggcgcacga gctgctcgcc gacctggaca
      541 aggaaaccgt cgactgggta ccgaactacg acggcaccga gcagattccg gcggtcatgc
      601 cgaccaagat ccccaacctg ctggtcaacg gttccagcgg tatcgccgtg ggcatggcga
      661 ccaacatccc gccgcataac ctcggcgaag tcatcgatgg ctgtctggcg ctgatcgata
      721 acgcggacat cagcatcgac gagctgatgc gctacattcc cggtccggat ttccccacgg
      781 caggcatcat caacggtcgt gccggcatca tcgaggccta tcgcaccggc cgcgggcgcg
      841 tcgtcatgcg ggcccgcgtc gaggtcgagg acatcgacaa ggtcggcggc cgccagcagc
      901 tggtggtcac cgagctgccc taccagctga acaaggcgcg gctgatcgag aagatcgccg
      961 agctggtcaa ggagaagaag ctcgagggca tcagcgagct gcgcgacgag tcggacaagg
     1021 acggcatgcg cgtggtcatc gagctgcgcc gcggcgaagt gccggaggtg gtgctcaaca
     1081 acctctatgc gcagacccag atgcagagcg tgttcggcat caacgtggtg gcgctggtcg
     1141 acggccagcc gcgcacgctg aacctcaagg acatgctcga ggtgttcgtc cgtcaccgcc
     1201 gcgaagtggt gacccggcgc accgtgtacg agctgcgcaa ggcgcgcgag cgcggccaca
     1261 tcctcgaagg ccaggcggtg gcgctgtcca acatcgaccc ggtgatcgaa ctgatcaagc
     1321 agtcgccgac cccggccgag gccaaggagc gtttgatcgc caccgcctgg gaatccagcg
     1381 cggtggaagc catggtcgag cgcgccggcg ccgactcctg ccgcccggac gacctcgatc
     1441 cgcagtacgg cctgcgcgag ggtaagtact acctgtcgcc ggagcaggcc caggccatcc
     1501 tcgagctgcg cctgcaccgc ctgaccggtc tggagcacga gaagctgctg gccgagtacc
     1561 aggagatcct cactcagatc ggcgagctga tccgcatcct gagcaatccc gtgcgcctga
     1621 tggaagtcat ccgcgaggag ctggagaagg tcaaggccga cttcggcgat gcgcggcgca
     1681 ccgagatcgt cgcctcccag gtcgatctga ccatcgccga cctgatcacc gaagaagagc
     1741 gcgtggtgac catctcccac ggcggctacg ccaagtccca gccgctggcc gcctaccagg
     1801 cgcagcgccg cggcggccgc ggcaagtcgg cctccggggt gaaggacgag gactacatcg
     1861 agcacctgct ggtcgccaac agccacgcca ccctgctgct gttctccagc aagggcaagg
     1921 tctactggct gcgcaccttc gagattcccg aggcttcgcg caccgcgcgt ggccggccgc
     1981 tggtcaacct gctgccgctg gacgagggcg agcgcatcac cgcgatgctg caggtcgacc
     2041 tcgaggcgct gcgcgcccag gcgccggaag gcgacgagga gctggacgac agcgaaggcc
     2101 tggtgatcga gggcgagctg gtcgaggcgg aaagcgccga ggacagcgat gccgaggtcg
     2161 ccgacgacga gcaggacgag ccgaccggcg cctacatctt catggccacc gccttcggta
     2221 ccgtgaagaa gaccccgctg gtgcagttca gcaagccgcg cagttcgggc ctgatcgccc
     2281 tgaagctgga agagggcgac accctgatcg ccgccgccat caccgatggc gccaaggagg
     2341 tcatgctgtt ctccgacggc ggcaaggtga tccgcttcaa ggagaagcat gtgcgcacca
     2401 tgggccgtac cgcccgcggc gtgcgtggca tgcgtctggg cgagggccag cagctgattt
     2461 ccatgctgat ccccgaggcg caggcgcaga tcctcaccgc ttccgagcgc ggctacggca
     2521 agcgcaccgc catcgaggag ttcccgcagc gcggccgcgg cggccagggc gtgatcgcca
     2581 tggtcagcaa cgagcgcaat ggcaagctgg tcggcgccat ccaggtgcag gacggcgagg
     2641 agatcatgct gatctccgac cagggcaccc tggtgcgtac ccgtgtcgac gaagtgtcct
     2701 cgctgtcgcg caacacccag ggcgtgaccc tgatcaagct ggccagcgac gagaccctgg
     2761 tcggcctgga gcgcgtgcag gagccgtcgg acaacggtga ggacgaggcg ctggacgacg
     2821 agcacgcgga cagtgcggtc gacaccggcg cagaagtggc ggcggccgac gagagcgcgc
     2881 cgcaggccgg cgaagagtaa gccatcgcag gcgggatgcg tcccgcctgc cgtcgcagca
     2941 agatcggcga aggacgtacg gcgtcccttg cccaacaact gagagtggat gtgagcatgc
     3001 gagcccataa tttctgcgcc ggcccggccg cgctgcccga agccgttctg cacaaggccc
     3061 aggccgaact tctcgactgg cacggcaagg gcctgtcgat catggagatg agccaccgca
     3121 gcgacgacta cgtcgccgtc gccgagaagg ccgagcagga cctgcgcgac ctgctggcca
     3181 ttccgtcgaa ctacaaggtg ctgttcctgc agggcggcgc cagccagcag ttcgcccaga
     3241 tcccgctgaa cctgctgccc gaagacggca tcgccgacta cgtcgacacc ggcatctggt
     3301 cgaagaaggc cctcgaggag gcgcgccgct tcggcaacgt caacgtcgcc gccagcgcca
     3361 aggcctacga ctacttcgcc attcccgggc agaacgactg gcagctgtcg aagaacgccg
     3421 cctacctgca ctacgccagc aacgagacca tcggtggtct gcagttcgac tgggtgccgg
     3481 acctcggcga cgtgccgctg gtggtcgaca tgtcctcgga catcctctcc cggccgatcg
     3541 acgtgtcgca gttcggcctg atctacgccg gcgcgcagaa gaacatcggc cccagtggcc
     3601 tggtggtggt gatcgtccgc gaggacctgc tcggcaaggc gcgcagcatc tgcccgacca
     3661 tgctcgacta caaggtcgcc gccgacaacg gctcgatgta caacaccccg gcgaccttct
     3721 cctggtacct gtccggcctg gtgttcgagt ggctgaagga gcagggcggc gtcgaggaaa
     3781 tggagcggcg caaccgcatc aagcaggagc tgctgtacgg cgccatcgac gccagcgagt
     3841 tctacaccaa cccgatcgcc aagaacgccc gctcgtggat gaacgtgccg ttccgcctgg
     3901 ccgacgagaa gctggacaag gctttcctcg ccggcgccga ggcgcgcggc ctgctcaacc
     3961 tcaagggcca tcgttcggtc ggcggcatgc gtgcctccat ctacaacgcg gtcggtctgg
     4021 acgcggtgca ggcgctggtc ggctacatgg ccgaattcga gaaggagcac ggctgatgtc
     4081 cgaaaaggag cagttgcagg ccctgcgcgt gcgcatcgac agccttgacg agcagattct
     4141 cggcctgatc agcgagcgcg cgcgctgcgc cgaggaagtg gcgcgggtga agatggccgc
     4201 gctgcagcct ggcgagaagc cggtgttcta ccgtcccgag cgcgaagcct gggtgctcaa
     4261 gcacatcatg gagctcaatc aggggccgct cggcaacgag gaagtggcgc gcctgttccg
     4321 cgagatcatg tcgtcctgcc tggccctcga gcacccgctc aaggtcgcct acctcggccc
     4381 ggaaggcacc ttcacccagg ccgcggcgct caagcacttc ggcaatgcgg tgatcagcca
     4441 gccgatggcg gcgatcgacg aggtgttccg cgaagtggcc gccggcgcgg tcaacttcgg
     4501 cgtggtgccg gtggaaaact ccaccgaggg ggcggtcaac cacaccctcg acagcttcct
     4561 cgagcacgat ctggtgatct gcggcgaggt ggagctgcgc atccaccacc acctgctggt
     4621 cggcgagaac accaagaccg acaagatttc gcgcatctat tcccacgccc agtcgctggc
     4681 gcagtgccgc aagtggctgg acgcgcacta cccgaacgtc gagcgcgtcg cggtatccag
     4741 caacgccgat gccgccaagc gggtgaagag cgagtggaac agcgcggcga tcgccggcga
     4801 catggccgcc cagctgtatg ggctgaccaa gctggccgag aagatcgagg atcgcccgga
     4861 caactccacg cgctttttga tcatcggtaa tcaggaagtc ccgccgactg gcgacgacaa
     4921 gacctcgatc atcgtctcca ccaacaacaa gcccggcgcg ctgcacgacc tgctggtgcc
     4981 gttccaggcc cgcggcatcg acctgacccg catcgagacc cgcccgtcgc gcagcggcaa
     5041 gtggacctac gtgttcttca tcgacttcgt cggccaccag caggacccgc aggtcaagga
     5101 cgtgctggag cagatcagcc aggaatccgt ggccgtcaag gtgctcggct cctaccccaa
     5161 cgccgtactg taaaccgcgt ctgcgccggt ccgggtcgcc cttgccccgc ggccggcgat
     5221 ccttgccgga gacaaatcga tgagctgtga tttcctcgcc ctggccgtgc cgggtgtgca
     5281 gaagctgtcc ccctacgtgc cgggcaagcc ggtcgacgag ctggcccgcg agctgagcct
     5341 cgatccggcc ggcatcgtca agctggccag caacgagaac ccgctgggcc cgtcgcccaa
     5401 ggcgctggag gcgatccgcg ccgagctggg cgacatcacc cgttatccgg atggcagcgg
     5461 ctaccagctc aaggccaagc tggccgcgcg cttcgggctg agcccggcgc agatcaccct
     5521 gggcaatggc tccaacgaca tcctcgacct ggtcgcccgc gcctggctgg cgccggggct
     5581 gaacgcggta ttcagccagt acgccttcgc cgtctacccg atttccaccc aggcggtcgg
     5641 cgccgagggc cgcgccgtgc cggccaagga ctacggccac gacctcgagg ccatgctggc
     5701 ggccatcgac gccaacaccc gcgtggtgtt catcgccaac cccaacaacc cgaccggcac
     5761 ctggttcggc ccggacgccc tggagtcctt cctgtcgcgc gtgccggccc atgtgctggt
     5821 ggtgctcgac gaggcctaca tcgagtttgc cgagggcgac gagctgccca acggcctcga
     5881 gtacctcgcc cgttacccga acctgctggt ctcgcgcacc ctgtgcaagg cctacggcct
     5941 ggccggcctg cgcgtcggct attccgcctc ctcgccgcag gtggccgacg tgctcaaccg
     6001 cgtgcgccag ccgttcaacg tcaacagcct ggccctggtc gccgcctgcg ccgcgctgga
     6061 cgatgccgac tacctggccg caggccggcg caacaacgtt gccggcatgg cccagctgga
     6121 agccggcttc cgcgcactgg gtctcaagtg gattccgagc aagggcaact tcatcgccgt
     6181 cgacttcggt cgcgatgccg cgccgatcaa ccaggcgctg ctgcaggagg gcgtgatcgt
     6241 gcgtccggtg gccggctacg gcatgccgac cttcctgcgc gtgtccatcg gcctgccggc
     6301 ggagaaccag cgcttcctcg aggcgctggc caaggtgctc ggccgtggct gagccaggac
     6361 gtgcgcccct cgtcggccgc ctggtggtgg tcggcctcgg cctgatcggc ggctccttcg
     6421 ccaagggcct gcgccagcag ggcctgtgcc gcgaggtggt gggtgtcgat ctggatgccg
     6481 agtcgcgtcg cctggcggtg cagctgggcg tggtcgaccg ctgcgaaagc gagctggcca
     6541 cggcctgcgt cggcgccgac gtaatcatgc tggcggtgcc gatcctggcc atggagaagg
     6601 tgctggcgca gctggccgct cttgacctgg gcgatgccgt gctcactgac gtcggcagcg
     6661 ccaagggcaa cgtggtgcgc gccgcacgcc aggtgctcgg cgcgcacatc gctcgtttcg
     6721 tgcccggcca tccgatcgcc ggctccgagc agagcggggt ggaggcggcc aacggcgcgc
     6781 tgtaccgccg gcacaaggtg atccttaccc cgctggccga gaccgatgcg caggcgctgg
     6841 cgctggtcga ccgcctgtgg cgcgcgctgg gcgccgacgt cgagcacatg gccgtcgagc
     6901 accacgacga ggtgctcgct gccaccagcc acctgccgca cctgctggcc ttcggcctgg
     6961 tcgactcgct ggcccaccag agcgagaatc tggagatctt ccgctacgct gccggcggct
     7021 ttcgcgactt cacccgcatc gccggcagcg atccggtgat gtggcacgac atcttcctcg
     7081 ccaaccgcga ggcggtactg cgcattctcg accgttatcg cagcgacctc gacggcctgc
     7141 gcgaagcgat cgaccatgga gacgggcatc atctgctggg cgtattcacc cgcgcccgcg
     7201 tcgcccgtga gcatttcagc aaaattctgg cccaacgggc ctatgtggac gccatgcatt
     7261 ccaacgacct gatcttcctg gcccaaccgg gcggcagcct gagcggacgg attcgcgtcc
     7321 cgggcgacaa gtccatctcc caccgctcga tcatgctcgg ctcgctggcc gaaggcacca
     7381 ccgaggtgga gggcttcctc gagggcgagg acgccctggc caccatccag gccttccgcg
     7441 acatgggcgt ggtcatcgag ggcccgcacc acggccgcgt caccgtgcac ggcgtcggcc
     7501 tgcacggcct cagggcgccg gccggcccgc tgtacatggg caactccggc acctccatgc
     7561 gcctgctctc cggcctgctc gccgcccagc cgttcgacac cgtgctgacc ggcgacccgt
     7621 cgctgtccaa gcggccgatg aaccgcgtgg ccaagccgct gcgcgagatg ggcgcggtga
     7681 tcgagaccgg ccccgaaggc cgtccgccgc tgaacatcaa aggcggccag cgcctgaccg
     7741 gcatgcacta ccagatgccg atggccagcg cccaggtcaa gtcctgcctg ctgctggccg
     7801 gcctgtacgc cgccggcgag accgcggtga ccgagccggc gccgacccgc gaccacaccg
     7861 agcgcatgct gcgcggcttc ggctatccgg tcgaggtgca cggtgccacc gccaaggtgg
     7921 aaagcggcca caagctctcc gccacccaca tcgaggtgcc ggcggacatt tcctcggcgg
     7981 ccttcttcct ggtggcggcg agcatcgccg agaactccga actggtgctc gagcacgtcg
     8041 gcatcaaccc gacccgcacc ggggtgatcg acatcctcaa gctgatgggc ggtgacatca
     8101 ccctggagaa ccagcgcatc gtcggcggcg agccggtggc cgatctgcgc gtgcgcagtg
     8161 ccaagctcaa gggcatcgag attcccgagg atctggtgcc gctggccatc gacgagttcc
     8221 cggtgctgtt cgtcgccgcc gcctgtgccg agggccgcac cgtgctgcgc ggcgccgagg
     8281 agctgcgggt caaggagtcc gaccgcatcc aggtgatggc cgacggcctc caggcctgcg
     8341 gcgtcaaggc cgagccgacg ccggacggca tcgtcatcga cggcggcccg atcggcggcg
     8401 gcgaagtgtg ggcgcacggc gaccaccgca tcgccatgtc gttcagcgtc gcctcgctgc
     8461 gcgctagcgc gccgatccgc atccacgact gcgccaacgt ggccacctcg ttccccaact
     8521 tcctcgccct gtgtgccgag gtcggcgtgc gcgtggccaa ggaggaacag caatgacccg
     8581 cctggctccg gtcgtcacca tcgacgggcc gagcggctcg ggcaagggca cggtgtgcag
     8641 tcggctggcg cgccagctgg gctggcacct gctcgactcc ggtgcgctgt atcgcctgct
     8701 ggcgctggcc gccggtcgcc atggcatcgc cctggacaac gaaaccgcgc tggagaccct
     8761 ggcggccaac ctggacgtgc agttcatcgc cgcccaggaa ggcctgcacg gccagcgcat
     8821 cctcctcgag ggtgaggagg tcggcgacga gctgcgtacc gagcaggccg gcgccggcgc
     8881 ctcgcaggtc gccgtgctgc cgggcgtgcg cagcgcgctg ctgcagcgcc agcgcgattt
     8941 tcgcgtcgac cccggcctgg tggccgacgg ccgcgacatg ggtaccgtgg tatttgccga
     9001 cgcgccgctg aagattttcc tcaccgcgag cgccgaggag cgtgcccgtc ggcgctacct
     9061 gcagttgaag gacaaggtat ccggtgttaa tctgacaagt ctgctagaag agatccgtgc
     9121 acgcgacgag cgtgacatgc agcgatcagt ggctccgctg aagccggcgt ccgacgccat
     9181 cctgctggac tccactgaac tctcgatcga gcaggtactg gaacgcatcc tcgccgaact
     9241 cgccctgcgc gatctggtgg gatgacccag atgaggtgga cgggaaacca gtcccattgc
     9301 cgcccgcctc catatgacaa cgaacccgta ccgactgggg tgcggatcgg gcatatttcc
     9361 tgtgcccttg atcaacaggt atgaacatga gcgaaagctt cgcagaactt tttgaagaaa
     9421 gcctgaaatc cctcgacatg cagccgggtg ccatcatcac cggcatcgtg gtcgacatcg
     9481 acggtgactg ggttaccgtg catgccggcc tcaagtccga gggcgtcatc ccggtcgagc
     9541 agttctacaa cgagcagggc gaactgacca tcaaggtcgg tgacgaagtc cacgtggcgc
     9601 tggacgccgt ggaagacggc ttcggcgaaa ccaagctgtc ccgcgagaag gccaagcgcg
     9661 ccgagtcctg gatcgttctg gaagctgcgt tcgctgccga agaagtggtc aagggcatca
     9721 tcaacggcaa ggtcaagggc ggcttcaccg tcgacgtcaa cggcatccgc gcgttcctgc
     9781 cgggttcgct ggtcgacgtg cgtccggtgc gtgacaccac ccacctggaa aacaaagagc
     9841 tcgagttcaa ggtcatcaag ctcgaccaga agcgcaacaa cgttgtcgtt tcccgtcgca
     9901 gcgtgctgga agccgagaac agcgccgagc gcgaagctct gctcgagtcc ctgcaggaag
     9961 gccagcaggt caagggtatc gtcaagaacc tcaccgacta cggtgcgttc gttgacctgg
    10021 gcggcgtcga tggtctgctg cacatcaccg acatggcctg gaagcgtatc aagcatccgt
    10081 ccgagatcgt caacgttggc gacgagatcg acgtcaaggt gctgaagttc gaccgcgagc
    10141 gcaaccgcgt ctccctgggt ctgaagcagc tgggcgaaga tccgtgggtc gccatcaagg
    10201 cgcgctaccc ggaaggcacc cgcgttcagg cgcgcgttac caacctcacc gactacggct
    10261 gcttcgcaga gctggaagaa ggcgtcgagg gtctggtaca cgtgtccgaa atggactgga
    10321 ccaacaagaa catccacccg tcgaaggtcg tcaacgtcgg cgacgaagtg gaagtcatgg
    10381 ttctggacat cgacgaagag cgtcgtcgta tctccctcgg catcaagcag tgcaagccga
    10441 acccgtggga agagttctcc ggccagttca acaagggcga ccgcatctcc ggcaccatca
    10501 agtcgatcac cgatttcggt atcttcattg gtctggacgg cggcatcgac ggcctggttc
    10561 acctgtccga catctcctgg aacgaagccg gcgaagaagc cgtgcgtcgc ttcaagaagg
    10621 gcgacgagct ggacaccgtc atcctgtcgg tcgatccgga gcgcgagcgc atctccctgg
    10681 gcatcaagca gctggaagac gatccgttct ccaactacgc tgccctgaac gagaaaggca
    10741 ccatcgtgcg cggcaccgtc aaggaagtcg acgccaaggg cgccatcatc accctggctg
    10801 acgaagtcga aggcgtgctg aaggcttccg aaatcagccg tgaccgcgtt gaagacgcgc
    10861 gcaacgtgct gaaggaaggc gaagaagtcg aagccaagat catcagcgtg gatcgcaaga
    10921 gccgcgtgat cagcctgtcc atcaagtcga aggacgtggc tgaagagaag gaagcgatga
    10981 aggaactgcg caagcaagac gtgagcgtgg gtccgaccac catcggcgac ctgatcaagg
    11041 cacaaatggg caaccagaac taagttctga ctgttcatga aaaagggcga cttcggtcgc
    11101 cctttttcgt ttctggcgat tcttccccgg cgcccggacc agactccgtg cgtccatggg
    11161 cgacttgcct gtgcggggcc gggcgttatc atggcggccc tgacttccac ggtgagcccc
    11221 catgtccgat gccatctctg ccggttccga acccagcgtc ctgtatgcca agctgcttgg
    11281 cgaaaccgca accatcacct ggcaggagct gcagcgcttc ttcgccggcg gcaacgtcct
    11341 gcgcgttgcc ggtgagctgg atctggtggc ggtcgccgag gcgctggccg aggatgacgg
    11401 tgcgaaggtg cagctctggc tgaccgctgg cagcctgcgc aagatggaag tggccgatgc
    11461 cgaggactac caggcgcgcg atccgcagct gtgggccgtc gtggtggcgc cctgggtgct
    11521 ggtgcaggag cgtgctgagg cgccgtcttt gcactgatcc gctgccagcg ggctgcgcat
    11581 gaaagtcgga ctagactgat tgcagtttct gccaagagga atgcaatcat gttcgagccc
    11641 ggtcacatac acgtcgccaa cccggtggct gccttaggcc tgccgaccta cgccgccgat
    11701 ctttattacg aagtgcgcac tggcgccaag gaggggccgg tggtgcactt ccgcatggcg
    11761 ggcgagatcg ccggaaagcc tttcgaggaa gaattcgaga tgtcccatga caccgtgttc
    11821 aacttcgcca gcacgatggg gggggttgct gccaagcacg ggatgccgat caaccacagc
    11881 ccggtcttgc gccaccacaa ggaatttgac gccatcttca aggatctgcg cgccaagctc
    11941 aaagtggccg tcaacgggcc gttgagcgcc catgacccga acgcagggac ctgagcgcgg
    12001 cggccgcctg cggctagtgg ctgacccagc gctgccgtgc ccagccgctc agtgcatcca
    12061 ccgccagcac cagcagcagc atggcgagga tcaccgtggc ggcctgggcc tgctggaaca
    12121 ggctcaggct gacgtagagc atctggccga ggccgccggc gccgacgaag ccgagcacgc
    12181 tggccatgcg gatgttgttc tcccagcggt acagcgagta ggcgatcagc tgcggccata
    12241 cccccggcag ggtgccgtag acgaaggcgg cgacgtgccc accaccggct aggcggatgg
    12301 cctcggctgg ctcggccggg gtgttctcca gcgcctcggc gaacagccgg ccgagcacgc
    12361 cagtggtgtg cagggccagg gccagggtgc cggcattcgg gcccaggccg gcggccagca
    12421 ccatcagcgc tgcccacacc agttccggaa tcgaacgcaa tgcattgagc acgaagcgcg
    12481 ccagcgcggc agcggcgcgg ccgcagcgtc cggctgccgg cagcgccagc aacaggccga
    12541 gcagggcggc gagcagggtg ccgagcgccg acatggccag ggtttccagg ctggcgcggc
    12601 cgatgctggc gaggtgcgcc gccgagaagt ccgccgggaa gaagccggcg gcgtagtcgc
    12661 ccatcagcgc cagccccttg gcgctggtca gtcccgagaa gtccaggccg agccaggcga
    12721 aggcggcggc gacggccagc aggatcgcca gcagcagggc gtagttgagc gcgcgcttca
    12781 tgccagccgg ctcctcagca aacggctgag ctggtcggcc agcagcacca gggcgaagaa
    12841 ggtcagcagc atgctggcca cctcgccgcc ggcgaacatg cgcatcgaca ggtcgatctg
    12901 ctggccgagg ccgccggcgc cgacgaagcc catcaccacc gaggcgcgga tcgcgcactc
    12961 ccagcggtac accgtgtagg agagcatctc cgccgccgcg ttgggcagca cgccgtaggc
    13021 gaacgccgtc aggcgcgggc tgccggcggc gagcagggcg cgcgccgggc gcggatcgac
    13081 cgactcgcag atttccgcat agaccttgcc gagcatgcca ctgtaggtga tggcgatggc
    13141 cagcaccccg gcggtcgggc cgaggccgac ggcgcgcacg aacagcagcg cccagacgat
    13201 ctcgggaatg ctgcgcagca cgatcagcag caggcgcacc ggccagcgca gcgcatttgc
    13261 ccaccagcgc ggccggccgc cgcgggccag cgcggagacc gacagcacgc gggtggccag
    13321 cagtgcgcag ggcacggcga gcagccaggc cagcgccatg ccggcggtgg cgatggccag
    13381 ggtttccagg gtggcgcggc cgagcagggc gaggaactcg ccgtcttggg ccggcggcca
    13441 gaaggcggcg aggaagctgc ccatggtcgc ggcattgccc gggtcgaaca gcacggcgag
    13501 gttcagctcg ctgagctgca ggccgggcca cagcagggcc agcgccagca gggtcagcag
    13561 caggcgcggc agggcggcgg gatcgcgcag gtcctccgct gccgtcttcg caggtgcagt
    13621 cgagtggctc agcatcgcgg gatggtcacc agctgtggcg cgggcatcgg cgatgcgggc
    13681 agcggcaggt cgagctgctc gttggcgtac agggcgtcca gcgcctgcgg ggtcacctca
    13741 tgggccggca ggtcgaaggc gatgcgcccg tcgcgcacac cgacgatgcg cgggaagtgc
    13801 gccagcgcca gttccaccgc atgcaggctg gccagcaggg tgacgccgcg ggattccgcc
    13861 tcgcgagtca gcacgccgag ggtatggctg gccagtaccg ggtccatggc cgacaccggc
    13921 tcgtcggcca gcaggagttc ggcctgctgg tacagcacgc gggcgatgcc gacgcgctgc
    13981 agctggccgc cagagagctg gtcgcagcgt tcgaacagct tgtcggccag gtcgaggcgc
    14041 gccagcgcgt cgcgggcgcc ggtcgagtcg agcgggtgca gcaggttgag caggcccttc
    14101 cacagcggcc actggccgag gcggccggcc agcaccgcgg tgaccacccg ctggcgcggt
    14161 ggcaacggcg ggctctggtg gat
//
LOCUS       DATCUN010000294         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_52592, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000294 DATCUN010000000
VERSION     DATCUN010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52592"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..802
                     /locus_tag="VIO83_11515"
     CDS             14..802
                     /locus_tag="VIO83_11515"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HEY8331939.1"
                     /translation="MQYSEDKGSIMGDANQPRWKSSKVRDGIEEIHIRKWNYFIDYIQ
                     EAFSPGSGWVYRGHRKDYYKLESTIDRKIVDKSKTTKLRQDQLNRFKMAIRGKRGANP
                     PQYDEPLEWWALGQHFELLTPLVDWTHSPYTAAFFAFANTGKDDTRKRVIIALNKSEV
                     EGRPELASMVRFYTPFSDENARVLGQNGLFSYSDSGEELEAVIAHEFAGSTAAIIKKI
                     HLPNAERISVLRGLEQMNINHLTLFPDLSGASNYANMQFELYSN"
     gene            complement(834..1688)
                     /locus_tag="VIO83_11520"
     CDS             complement(834..1688)
                     /locus_tag="VIO83_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8331940.1"
                     /translation="MPYLLIVTLLWAFSFSLIGEYLAGQVDSDFAVLARVVIAALVFL
                     PFTVWRGLPGRLLAGFWLAGALQFGVTYLCLYRSFTVLTVPEVLLFTVLTPIYVTLLD
                     DALARRFNPWALVAAVVAVSGGAIIRFKPLEGDYLLGFLLLQLANATFAAGQVLCRQL
                     LARYPVEQPLQRYFGHFFLGALVLALPSFLLFGNPAKLPHTAVQWGVLLWMGLFATAL
                     GMFWWVKGSTRVNAGTLAVMNELHVPAGLVVNLLIWNRDADLPRLAAGGAVILASLWL
                     NRLGRRLA"
BASE COUNT          359 a          602 c          618 g          243 t
ORIGIN      
        1 gtgaccggct cccatgcagt actcagaaga caagggctcg atcatgggcg acgcaaatca
       61 accgcgctgg aagtccagca aggtcaggga cggcatcgag gaaatccaca tcaggaagtg
      121 gaactacttc atcgattaca ttcaggaagc cttttcgccc gggtccggct gggtctatcg
      181 cgggcaccgc aaggactact acaagctcga gtcgaccatc gaccgcaaga tcgtcgacaa
      241 gtcgaagacc accaagctgc gccaggacca actcaaccgc ttcaagatgg ccattcgcgg
      301 caagcgcggg gcgaacccgc cgcagtacga cgagccgctg gagtggtggg cgctcggcca
      361 gcatttcgag ttgctgacgc cgctggtgga ctggacccat tcgccctata ccgcggcgtt
      421 ctttgccttc gccaacaccg gcaaggacga cacccgcaag cgggtgatca ttgccctgaa
      481 caagagcgag gtggaaggtc gtcccgagct ggccagcatg gtgcgcttct atacgccgtt
      541 ctccgacgag aatgcgcggg tgctcggcca gaacgggctg ttctcctatt ccgactccgg
      601 ggaggagctg gaggcggtga tcgcccacga gtttgccggc tcgaccgccg ccatcatcaa
      661 gaagatccac ctgcccaacg ccgagcggat cagcgtgttg cgcgggctcg agcagatgaa
      721 catcaaccac ctgacgctgt tccccgatct cagcggcgcg agcaactacg ccaacatgca
      781 gttcgagctg tactccaact agccgcgtgg agggccggcg cggccttgcc gcctcacgcc
      841 aggcgccgcc ccagccggtt gagccacagc gaggcgagga tcaccgcgcc gccggcggcg
      901 aggcgcggca ggtcggcgtc gcggttccag atcagcaggt tgaccaccag gccggccggc
      961 acgtgcagtt cgttcatcac cgccagggtg ccggcgttca cccgcgtgct gcccttgacc
     1021 caccagaaca tccccagtgc ggtggcgaac aggcccatcc acagcagcac gccccactgc
     1081 acggcggtgt gcggcagctt ggccgggttg ccgaacagca ggaacgaggg cagggcgagg
     1141 accagtgcgc cgaggaagaa gtggccgaag tagcgctgca gcggctgctc gaccgggtag
     1201 cgcgccagca gctggcggca cagcacctgg ccggcggcga aggtggcgtt ggccagttgc
     1261 agcagtagga agccgagcag gtagtcgccc tccagcggct tgaagcggat gatcgccccg
     1321 ccgctcaccg ccaccacagc ggcgaccagc gcccaggggt tgaagcgccg cgccagggcg
     1381 tcgtcgagca gggtcacgta gatcggcgtg agcacggtga acagcagcac ctcgggcacc
     1441 gtcagcacgg tgaagctgcg gtacaggcac aggtaggtga cgccgaactg cagcgccccg
     1501 gccagccaga agccggcgag cagccgcccc ggcaggccgc gccagacggt gaacggcagg
     1561 aacaccaggg cggcgatcac cacccgcgcc agcaccgcga agtcgctgtc cacctggccg
     1621 gccaggtatt cgccgatcag gctgaaggag aaggcccaca gcagggtgac gatcaacagg
     1681 tagggcatgg actgcgcgcc ttgcgacgaa acgggcgcgg atcataggcc gcgctggtgg
     1741 ggagcgtcga gccgggcgtc gtcgccgcac ccgagtggcg gcggcgacgc tgccggccgg
     1801 ttcaggcggt cgaaccgtgg cc
//
LOCUS       DATCUN010000295         3640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_202846, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000295 DATCUN010000000
VERSION     DATCUN010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202846"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1807)
                     /gene="glyS"
                     /locus_tag="VIO83_11525"
     CDS             complement(<1..1807)
                     /gene="glyS"
                     /locus_tag="VIO83_11525"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY8331941.1"
                     /translation="MTTQNLLVELFVEELPPKALQKLGDAFATVLGDQLRAQGLTSAD
                     TAVTAYASPRRLAAHVSHVALKAEDKAVQQKLMPVSVGLNAEGQATPALLKKLQALGA
                     DLSDPAAAVAALKRAPDGKAEALFYDSLVTGATLDTGLQKALDEAIAKLPIPKVMSYQ
                     LETDCELPGWSSVHFVRPAHGLVALHGTTVVPVKALGLTAGCTTQGHRFEALVSPVVV
                     PHSDQYAEVLQSDGAVIASFAARRAEIARQLAAAAAKVGNGARPIDDNALLDEVTALV
                     ERPNVLICSFEKEFLDVPQECLILTMKANQKYFPLLDAAGKLTNKFLVVSNISPEDTS
                     FVTGGNERVVRPRLADAKFFFDQDRKKTLASRVEGLAKVVYHNKLGTQGERVERVRAI
                     AKAIATQLGDAALVADADQAALLAKTDLVTDMVGEFPELQGTMGRYYALNDGLGEVIA
                     DAIEDHYKPRFAGDTLPRNTAGVVVALADKLETLVGMFGIGNLPTGDRDPFALRRHAL
                     GVIRMLVEKDLPLDLHALLAGAVPAFGDKITDATAPLADFIYDRLAGSLREQGYSAQE
                     VDAVLALRPQRLAQVPQFLAAVRAFAALPESAALAA"
     gene            complement(1831..2751)
                     /gene="glyQ"
                     /locus_tag="VIO83_11530"
     CDS             complement(1831..2751)
                     /gene="glyQ"
                     /locus_tag="VIO83_11530"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016782854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY8331942.1"
                     /translation="MLTFQQIILKLQSYWDAQGCALLQPYDMEVGAGTSHTATFLRAI
                     GPEPWKAAYVQPSRRPKDGRYGENPNRLQHYYQFQVVLKPAPANILELYLGSLEALGF
                     DLKKNDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCKPATGEITY
                     GLERLAMYLQGVDNVYNLTWTEGADGSRLTYGDVYKQNEVEQSTYNFEHSDADFLFTA
                     FNAHEKQAKHLMEQQLALPAYEQVLKAAHSFNLLDARGAISVTERAAYIGRIRNLARA
                     VAQSYYESRERLGFPMAPREWVAQMAKKAA"
     gene            complement(3167..>3640)
                     /locus_tag="VIO83_11535"
     CDS             complement(3167..>3640)
                     /locus_tag="VIO83_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8331943.1"
                     /translation="QNFNLFPHLTVGRNVMLAPTLVTSQPKDVARQNAQRLLARVGLA
                     EKFDAMPEQLSGGQQQRVAIARALAMNPEILLCDEVTSALDPELVGEVLQVVEGLADQ
                     GMTLIMVTHEMGFARKVSDRVVFMHQGRVHEMGVPEQLFGNPQTPELRQFLGKLH"
BASE COUNT          595 a         1150 c         1208 g          687 t
ORIGIN      
        1 cggcggccag ggccgcgctt tcgggcagcg cggcaaaggc gcgcaccgcc gcgaggaact
       61 ggggcacctg cgccagccgc tgcgggcgca gggccagcac ggcatcgacc tcttgcgcgc
      121 tgtagccctg ctcgcgcagg ctgccagcga ggcggtcgta gatgaagtcg gccagcggcg
      181 ccgtggcgtc ggtgatcttg tcgccaaacg cgggcacggc accggccagc agggcgtgca
      241 ggtccagcgg caggtctttt tcaaccagca tgcggatcac gcccagcgca tggcggcgca
      301 gcgcaaaggg atcgcggtcg cccgtgggca ggttgccaat gccgaacatc cccaccaggg
      361 tttccagttt gtcggccagt gccacgacca cgccggcggt gttgcgcggc agcgtgtcgc
      421 cggcaaaacg gggcttgtag tggtcttcaa tggcgtcggc aatcacttcg cccaggccgt
      481 cattgagcgc gtagtagcgc cccatggtgc cctgcagctc ggggaactcg cccaccatgt
      541 cggtcacgag gtcggtcttg gccagcagcg cggcttggtc agcgtcggcc accagagcgg
      601 catcgcccag ctgcgtggcg atggccttgg cgatggcgcg cacgcgctcc acgcgctcgc
      661 cctgcgtgcc cagcttgttg tggtagacca ccttcgcgag gccttccacg cgcgaggcca
      721 gcgtcttctt acggtcctgg tcgaagaaga acttggcatc ggccaggcgc gggcgcacca
      781 cgcgctcgtt gccgccggtc acaaagctcg tgtcctcagg gctgatgttg ctcaccacca
      841 ggaacttgtt ggtcagcttg ccggccgcgt caagcagcgg gaagtatttc tggttggcct
      901 tcatcgtgag gatgaggcat tcctgcggca cgtcgagaaa ctctttttca aacgagcaga
      961 tgagcacgtt gggccgctcg accagcgcgg tcacttcatc gagcagcgcg ttgtcgtcaa
     1021 tcgggcgcgc gccgttgccg accttggcgg cagcggcggc cagctggcgg gcgatttcgg
     1081 cgcggcgcgc ggcaaagctg gcaatcacgg cgccgtcgct ttgcagcact tcggcgtact
     1141 ggtcggagtg cggcaccacc accgggctca ccagggcctc aaaacggtgg ccttgcgtgg
     1201 tgcaaccggc ggtgaggccc agggccttga cgggcaccac ggtggtgccg tgcagcgcga
     1261 ccaggccgtg cgcggggcgc acaaagtgaa cgctgctcca gccgggcagc tcgcagtcgg
     1321 tctccagctg gtagctcatt accttgggga tgggcagctt ggcaatggcc tcgtccaggg
     1381 ctttttgcag gccggtgtcc agcgtggcgc cggtgaccag gctgtcgtag aacagcgcct
     1441 cggccttgcc atcgggcgcg cgtttaagtg cggccacagc ggcggcgggg tcggacaggt
     1501 cggcgcccag cgcctgcagt tttttcagca aggcgggcgt ggcctggccc tcggcattca
     1561 agcccacgct cacgggcatg agcttttgct gcaccgcctt gtcctcggcc ttgagggcca
     1621 catggctcac atgggccgcc agccggcgcg gcgaggcata ggcggtgacg gcagtgtctg
     1681 cggaggtcag gccttgcgcc ctgagctggt cacccagcac cgtggcgaag gcatcgccca
     1741 gcttctggag cgccttgggg ggcagctctt ccacaaacag ttcgaccaga agattttgcg
     1801 ttgtcatttt cagaggctcc gggtcttcga tcaggcggct ttttttgcca tttgtgccac
     1861 ccactcacgc ggcgccatgg ggaagcccag gcgctcgcgg ctctcgtagt agctttgcgc
     1921 cacggcgcgt gccaggttgc ggatgcggcc gatgtaggcg gcgcgctcgg ttacgctgat
     1981 ggcgccccgc gcatccagca ggttgaagct gtgcgcggcc ttgagcacct gctcgtaggc
     2041 cggcagcgcg agctgctgct ccatgaggtg cttggcctgc ttttcgtgcg cattgaaggc
     2101 ggtgaacaaa aagtcggcgt cgctgtgctc gaagttgtag gtggattgtt ccacctcgtt
     2161 ttgcttgtac acatcgccgt aagtcagcct ggagccgtcc gcgccttcgg tccaggtcag
     2221 gttgtagacg ttgtccacgc cctgcaggta catggccaag cgctccaggc cgtaggtgat
     2281 ctcgcccgtg gcgggcttgc agtcgatgcc gcctacctgc tggaagtagg tgaactgcgt
     2341 gacttccatg ccgttgagcc agacctccca gcccaggccc caggcgccga gcgtggggtt
     2401 ttcccagtcg tcttcgacaa agcggatgtc gttctttttc agatcgaagc ccagcgcttc
     2461 gagcgagccg aggtacagct ccaggatgtt ggccggcgcg ggcttgagca ccacctggaa
     2521 ctggtaatag tgctgcaggc gattggggtt ctcgccatag cggccatctt tggggcggcg
     2581 gctgggctgc acgtaggcgg ccttccaggg ctcggggcca atcgctctca aaaacgtagc
     2641 agtgtgcgac gtaccggcgc ccacttccat gtcgtagggt tgcaaaagcg cgcagccctg
     2701 ggcgtcccag tacgactgca gtttcagaat gatttgttgg aatgtcagca tgacaagttt
     2761 ggatgcgtcg caagcaggca cggccggtgc ccagggatcg gaaggcagtt caggctgcgc
     2821 aaacaatgat tttaaggtgc ggcacggcga tttgccgttg tgtccgcggc cggctccgcc
     2881 aaggctacac gccgattcag gaatggcgcc gctttcgctg gcccgatcca ccccgcgaaa
     2941 tcgcccccct atgggcctga tgaaccgcca tcaccccgcc aaccatggag attctggaat
     3001 agcacgcagg ctcgcgctcc atccttggcg cgtacgcggc aaggcttatg gcatggagcg
     3061 cagccacgcc accggcgtct tggtggccca caaggccatc aaattcaata gcacttaccg
     3121 cttactccat aagcggtaga ggccattttc accagaaaac caggcgtcaa tgcagtttgc
     3181 caagaaactg gcgcagctcg ggtgtctgcg ggttgccaaa cagttgttcg ggcacgccca
     3241 tctcgtgcac acggccctgg tgcatgaaca ctacacggtc gctcaccttc cgggcaaaac
     3301 ccatttcgtg ggtcaccatg atcagcgtca tgccctggtc agcgagtcct tcgaccactt
     3361 gcaacacctc gccgaccagt tccgggtcga gtgccgacgt gacctcgtcg catagcagga
     3421 tctccgggtt catggccagc gcacgggcga tggccacgcg ctgctgttgg ccgcccgaga
     3481 gctgttcggg catggcatcg aacttttccg ccagaccgac gcgcgccagc agtcgctggg
     3541 cattctggcg cgccacgtcc ttgggctggc tggtgacgag cgtgggggcc agcatcacat
     3601 tgcggcccac cgtgaggtgc ggaaacaggt tgaagttctg
//
LOCUS       DATCUN010000296         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_108850, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000296 DATCUN010000000
VERSION     DATCUN010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108850"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VIO83_11540"
     CDS             <1..1065
                     /locus_tag="VIO83_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HEY8331944.1"
                     /translation="RSVYNTILAWRTEAFQQEKTRIGYKEASARLTALKKDPDRAYLN
                     EVSCVPLQQCLRHQQQAFQAFLDKRAGYPTTKSKKHRQSAEFTASAFKYQDGQLFLAK
                     CKESLDIRWTKKRETLPSAPSTVTVSRDTAGRYFVSLLCEVETELLPVTPRTTGIDLG
                     LKALFVTAEGEQIDNPRHTAKYAARLAKAQRALSRKQRGSKNRAKARQKVARLHTKIA
                     DTRLDGLHKLSRRVINENQVVCVESLAVKNLVRNRRLSKSIADAGWGELVRQLEYKAA
                     WAGRQLVKLDRWFPSSKRCSCCGHTLASLPLKVRDWTCPACGSEHDRDLNAAINIKAA
                     GLAVLALGEDVSGIGCVPLS"
     gene            complement(1186..2001)
                     /locus_tag="VIO83_11545"
     CDS             complement(1186..2001)
                     /locus_tag="VIO83_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016567780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4892 domain-containing protein"
                     /protein_id="HEY8331945.1"
                     /translation="MKAWRPALAVAALLASPAASADVPGSSDLEFLPRFARAEIVDYR
                     QEDSVERIYPQSALRRISGRLRVDGEIDVVGRLSALTYELPAGHSSGDAFAQARTALL
                     AQGALPLHWCEGRECGASSLWANNVFGNARLYGPDEQQAYLLLQLAAPRQNSLLVLYA
                     ITRGTRRAYLHVEQLEANAPLGEHLLPNAETLLRQLKTAGVLELAQLPGEPADPWLAL
                     LARVLRLDTGVRLLLSGRSAPAWRDALIEGGTRAVRLESAVSEAPGLRVEWLR"
     gene            complement(2146..>2394)
                     /locus_tag="VIO83_11550"
     CDS             complement(2146..>2394)
                     /locus_tag="VIO83_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003097867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8331946.1"
                     /translation="TLPSPLRLSPAQAEAFARALRCPVSLVLAEEGLPVQRAEWLALI
                     ESLPIALHRLPGGHHLHLDDEAGATAVADCFKALFAAP"
BASE COUNT          428 a          817 c          781 g          368 t
ORIGIN      
        1 cgctccgtct acaacacgat cctggcttgg cgcaccgagg cgtttcagca agagaaaaca
       61 cgcataggct acaaggaagc cagcgcccgg ttgaccgcgc tgaagaaaga cccggatcgg
      121 gcctatctca acgaggtgtc ctgtgtgccg ctgcagcagt gcctccggca ccagcagcag
      181 gcgttccaag cgttccttga caagcgcgcc ggctacccga cgacgaaatc gaagaaacac
      241 cgtcagtcgg ctgagttcac cgcctctgcc ttcaagtacc aggatggcca attgttcctc
      301 gccaagtgca aggagtcgct ggacatccgc tggaccaaga agcgggagac gttgccctct
      361 gcaccgtcga cggtgacggt ttcccgcgat accgccggcc gttactttgt ctcgctgctg
      421 tgcgaggtag aaaccgagct gctgccggtg acaccgcgga cgaccggcat cgacctcggc
      481 ctgaaggcgc tgttcgtcac cgctgaaggc gaacagatcg acaacccgcg ccacaccgcg
      541 aagtatgctg cccgcctggc gaaagcccag cgggcgctga gccgcaagca acggggctcg
      601 aagaaccggg ctaaagcccg acagaaggtc gcccgcctgc acacaaaaat tgccgatacc
      661 cgactcgatg gcttgcataa gctgtcgcgc agagtgatca acgaaaacca agtggtctgt
      721 gtcgagtcgt tggcggtgaa aaacctggtc cgcaaccggc gcctgagcaa gtccatcgcc
      781 gatgccggct ggggcgagct ggtccgccag ctcgagtaca aggcggcatg ggccgggcgc
      841 cagctggtca agctggaccg ctggttcccc agcagcaaac gctgttcgtg ctgcggtcac
      901 accctggcct cgctgccgct gaaggtccgc gactggactt gtccggcgtg tggctcggaa
      961 cacgaccgcg atctgaacgc cgcgatcaac attaaagccg ccgggctggc ggtgttagcc
     1021 cttggagagg acgtaagtgg catcggttgt gtaccgctgt cctgatcttg gtgaattggg
     1081 aatctccgtc cgtcagggcg gagagcagtc aacgggccat cccttgcaac aggctgccgg
     1141 ccgccgccgg cgaaaaaacc acacccgccg ccacgctgca gcggctcaac gcaaccactc
     1201 cacacgcaat cccggcgcct cgctcacggc gctttccagc cgcacggcgc gggtaccgcc
     1261 ttcgatcagg gcatcgcgcc aggccggcgc gctgcggccg ctgagcagca ggcgcacgcc
     1321 ggtatccaga cgcaggacac gggccagcag ggccagccag ggatcggccg gctcgcccgg
     1381 cagctgcgcc agttccagca caccggcggt cttcagctgg cgcagcaggg tttccgcgtt
     1441 gggcagcaga tgctcgccca gcggcgcatt ggcttccagt tgttcgacat gcaggtaggc
     1501 acgccgcgtg ccacgggtga tcgcatagag caccagcagg ctgttctgcc gcggcgcggc
     1561 caactgcagc aacaggtagg cctgttgctc gtcggggccg tacagccgcg cattgccaaa
     1621 cacgttgttg gcccacaggc tgctggcgcc gcattcgcgc ccctcgcacc agtgcagcgg
     1681 cagcgcgccc tgtgccagca gcgcggtgcg cgcctgggcg aaggcgtcgc cgctgctgtg
     1741 cccggccggc agctcgtagg tcagcgcgct caggcggccg accacgtcga tctcgccatc
     1801 cacccgcagg cggccgctga tgcgccgcaa ggcactctgc gggtagatgc gctcgacgct
     1861 gtcctcctgc cggtagtcga cgatttcggc gcgggcaaag cgcggcagga actccaggtc
     1921 gctgctgcct ggcacatccg cgctcgccgc cggactggcc agcagcgcgg cgacggccag
     1981 ggcgggtctc catgccttca tcggatcgtc ttcgtcggca cggcccgcag tgcgagctcg
     2041 aaggcaacgg acatcctgcg gcccttggcg gttccggcgg tatactgctc gatcatgcaa
     2101 ttctcccgtg caatcccgcc agcctcgaca gttgcccggc gcaagtcaag gcgcagcgaa
     2161 cagggctttg aaacagtctg ctactgcagt tgcgccagcc tcgtcgtcga ggtgcaggtg
     2221 atggcctccg ggcaggcggt gcagcgcgat cggcaggctc tcgatcagcg ccagccactc
     2281 ggcacgctgc acgggcagcc cctcctcggc cagcaccagg ctgaccgggc agcgtagcgc
     2341 gcgggcgaaa gcctcggcct gggccgggct caggcgcagc ggcgacggca gggt
//
LOCUS       DATCUN010000297         4886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_108922, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000297 DATCUN010000000
VERSION     DATCUN010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108922"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VIO83_11555"
     CDS             complement(<1..746)
                     /locus_tag="VIO83_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8331947.1"
                     /translation="MLKLHFKDGRQAPIWLVEERFSIGEDRRNQLVLAEPGIGAFHAE
                     IIQRDGLYFLRDCDSAGGTFVNGQRIATQYQLRPEDQLRIGSVELLLLDPGKAVVRPD
                     ATVRWYLQVNSGELLGKKFQLQPGSLGVGRSSKCELCFGDPELSRRHGEFFLKDDVLE
                     VKDLASANGVYVNRQKVDTAVLTPGDEVRMGSLSLLVIGPKVGARPLEEEDEDATQFM
                     KVVDLPKVDKPQAAGRAPAANPLRAAAQAEA"
     gene            complement(794..2530)
                     /locus_tag="VIO83_11560"
     CDS             complement(794..2530)
                     /locus_tag="VIO83_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY8331948.1"
                     /translation="MSDSALVIPGYTLHGPLGKGGMAEVHLATQQSLQRKVAIKILSK
                     SEDQAFIQRFIKEGHLVASLHHPSIITIYDIDQLADGRHYLAMEFLPGGDLARHKGEL
                     FAPERALAIVRQIASGLAVVHDKGLVHRDVKPANILFRGDGTAVLTDFGVAKDLDLDS
                     ELTQFGIAVGSPAYSSPEQAQCLPLDARSDIYSLGVILLEMLTGSNPFRGGNYTQTVM
                     NHVQMPAPALPAALAGCQVLLEHMLAKDPEQRFADCRALLAALDQVELTDPDETRIGP
                     AVGLTATRAETPLAPPQVKAAQAAKARRSVLPILLSVLALLVTLTAGGLYWHQKSQIN
                     DLLAQAEQRLAAGQLVEPAEGSAEHYFRAVLAIDARNQAALGGLERVKAARIAGFLAL
                     GEQRLGEGKLLEPVQDSADHYFNQALLLDAGNAGALDGLRRVLDARIAANLARAEQSL
                     AEQRLLQPEDDSAVHYYRQILEWAPGHEQAVAGLQRVAMLYRDLANAAYRKGNFPGAL
                     AMIDNGLQVEPQHPQLLRMRDEHQALLSSARAAKASQASRPAPRQSTQQGQPGQEESN
                     PIKRVWTNLFGN"
     gene            complement(2548..3336)
                     /locus_tag="VIO83_11565"
     CDS             complement(2548..3336)
                     /locus_tag="VIO83_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014597348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEY8331949.1"
                     /translation="MFITHPAPGPAYAALTAVGRVRQHNEDAILCCPRFNLWAVADGM
                     GGHQRGEVASALALKTLQSQIRRGEDLLGAIHGANAAILAEASQDDSQRGMGTTLVAV
                     CFSGADFQLAWVGDSRAYRVGATGIEQLSRDHSWVQAMVDAGRMTPEEASCHPRRNIV
                     TQCLGQADQELQVDQVQGRLGSGELLLLCSDGLTGELSDTEIQQLCASAATLDALVDD
                     LVESANRKGGKDNISCIVLGLVAPAPAEERPRSFLDKLLKPAKS"
     gene            complement(3534..>4886)
                     /gene="putA"
                     /locus_tag="VIO83_11570"
     CDS             complement(3534..>4886)
                     /gene="putA"
                     /locus_tag="VIO83_11570"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEY8331950.1"
                     /translation="MDRLAAAFPHWSATPVAERAAVLERLAYLLEVHRAELLALCCLE
                     AGKTLQDGIDEVREAVDFCRYYAAQARERLLRDELPGPTGERNELFYAGRGVFACISP
                     WNFPLAIFLGQIAAALVAGNTVLAKPAEQASLIAMRAVELLLEAGLPPAVLALLPGDG
                     ATLGAAFCSDARLAGVCFTGSTDTARLINRQLADKPGALAVLIAETGGQNAMIVDSTA
                     LPEQVVKDAVQSAFTSAGQRCSALRVLYVQEDIAPRVIELLAGAMAELRVGPPDRRAT
                     DVGPLIDGEARATLLAHIDRLKGEGRLIAETPLPAGLDGHFVAPVAFAIEGIHALERE
                     HFGPVLHVVRFAAGELEQVVAAINATGYGLTLGVHSRNDDTVRRIERLARVGNLYVNR
                     NQIGAVVGVQPFGGLGLSGTGPKAGGPNYLLRFVSERCTATNTAAVGGNAALLALGND
                     "
BASE COUNT          734 a         1694 c         1691 g          767 t
ORIGIN      
        1 gcttcggcct gcgccgcggc gcgcagcgga ttggccgccg gcgcccggcc ggccgcctgg
       61 ggcttgtcca ccttcggcag gtcgacgacc ttcatgaact gggtggcgtc ctcatcctcc
      121 tcctcgagcg gtcgagcccc caccttgggg ccgatcacca gcaggctcag cgaacccatg
      181 cgcacctcgt caccgggcgt cagcaccgcg gtgtcgacct tctgccggtt gacgtacacg
      241 ccattggccg aggccaggtc cttgacctcc agcacatcgt ccttgaggaa gaattcgccg
      301 tgccggcgcg acagttccgg atcgccaaag cacagctcgc acttgctcga acgaccgacg
      361 cccaggctgc ccggctggag ctggaacttc ttgcctagca gctcgcccga gttcacctgc
      421 agataccagc gcacggtggc atcggggcgc accacggcct tgcccggatc gagcagcagc
      481 agctcgacgg agccgatgcg cagctgatcc tccgggcgca gctggtactg ggtggcgatg
      541 cgctggccgt tgacgaaggt gccgccggcg ctgtcgcagt cgcggagaaa gtagaggccg
      601 tcgcgctgga tgatctcggc atggaaggcg ccgatcccgg gttcggcgag caccagctgg
      661 ttgcgccgat cctcgccgat gctgaagcgc tcttcgacca gccagatggg ggcctggcga
      721 ccgtccttga aatgcagttt gagcatatga agcctgtcct gttcacgtcc cgccggtcat
      781 ggcggcatgc ctgtcagtta ccgaatagat tggtccacac ccgcttgatc ggattgctct
      841 cttcctgccc cggctggccc tgctgcgtgg actgccgcgg cgccggccgg ctggcctggg
      901 atgccttggc ggcacgcgcc gaactcagca acgcctggtg ttcgtcgcgc atcctgagca
      961 gctgcggatg ctgcggctcc acctgcaggc cgttgtcgat catcgccagg gcgccgggga
     1021 agttgccctt gcggtaggcc gcattggcca ggtcgcgata cagcatggcc acgcgctgca
     1081 gcccggccac ggcctgctcg tgccccggcg cccattcgag aatctgccgg tagtaatgca
     1141 ccgcactgtc gtcttcgggc tgcagcagac gctgctcggc gaggctctgc tcggcgcggg
     1201 cgaggttggc ggcgatgcgc gcgtccagca cccggcgcag cccgtcgaga gcgccggcat
     1261 tgccggcgtc cagaagcagg gcctggttga agtaatggtc ggcgctgtcc tgcaccggct
     1321 ccagcagctt gccctcgccc aggcgctgct cgcccagcgc gaggaagccg gcgatgcgcg
     1381 cggccttgac ccgctcgaga ccgcccaggg ccgcctggtt gcgcgcatcg atcgccagca
     1441 ccgcgcggaa gtagtgctcg gcgctgccct ctgccggctc caccagttgt ccggcggcca
     1501 gacgctgctc ggcctgggcc agcaggtcgt tgatctggct cttctggtgc caatacaggc
     1561 cgccggcggt cagggtgacc agcagcgcca gcacactgag cagtatcggc agcaccgacc
     1621 tgcgcgcctt ggcggcctgc gcagccttga cctggggcgg agccagcggg gtctccgctc
     1681 gcgtggcggt caggccgacc gccgggccaa tgcgggtttc gtccggatcg gtcagctcga
     1741 cctggtccag cgccgccagc agcgcccggc agtcggcaaa gcgctgctcc gggtccttgg
     1801 ccagcatgtg ttcgagcaga acctggcagc ccgccagggc agccggcagg gccggtgctg
     1861 gcatctgcac gtggttcatc accgtctggg tgtagttgcc gccgcggaag ggattgctgc
     1921 cggtgagcat ctccagcagg atcaccccca ggctgtagat gtcgctgcgc gcgtccagcg
     1981 gcaggcactg cgcctgctcc ggactgctgt aggccgggct gccgacggcg ataccgaact
     2041 gggtcagttc gctgtcgagg tcgaggtcct tggccacgcc gaagtcggtg agcaccgcgg
     2101 tgccgtcgcc gcggaacagg atgttggccg gcttcacgtc gcgatgcacc aggcccttgt
     2161 cgtgcaccac ggccagacca ctggcgatct ggcgaacaat ggccagcgcg cgctccggcg
     2221 cgaacagttc gcccttgtgc cgggccaggt cgccgccggg caggaactcc atggccaggt
     2281 agtggcggcc gtcggccagc tggtcgatgt cgtagatggt gatgatcgag ggatgatgca
     2341 gagaggcgac cagatgcccc tccttgatga agcgctggat gaatgcctgg tcttcggact
     2401 tgctgagaat cttgatcgcc accttgcgct gcagcgactg ctgggtggcc aggtggactt
     2461 cggccatgcc gcccttgccc agcgggccgt gcagggtgta gccgggaatt acgagtgcgc
     2521 tgtcgctcat gggagtggga cgccgaatca ggattttgcg ggtttcaaca atttgtcgag
     2581 aaagctgcgc ggccgctctt cggcgggcgc tggcgccacc aagcccagca cgatgcagga
     2641 gatgttgtcc ttgccaccct tgcggttggc cgactcgacc agatcgtcga ccagggcatc
     2701 gagcgtggcg gcgctggcgc acagctgctg gatctcggta tcgctcagtt cgccggtcag
     2761 gccgtcgctg cacagtagca gcagctcgcc actgcccagg cgtccctgca cctgatcgac
     2821 ctggagttcc tggtcagcct gaccgagaca ctgggtgacg atattgcgcc gggggtggca
     2881 gctagcctcc tctggcgtca tccgcccggc atcgaccatt gcctgtaccc agctgtgatc
     2941 gcggctcaac tgctcgatgc cggtggcccc cacccggtag gcgcggctat cgccaaccca
     3001 ggccagctgg aaatcggcac cggaaaagca cacggcaacc agggtggtgc ccatgccgcg
     3061 ctggctatca tcctggctgg cctcggcgag aatcgccgca ttggccccat ggatggcgcc
     3121 cagcagatcc tcgccacggc ggatctggct ttgcagggtc ttcaacgcca gggcactggc
     3181 cacctcgccg cgctggtggc cgcccatgcc atcggccacc gcccacaggt tgaaccgcgg
     3241 gcagcacagg atggcgtctt cgttgtgctg acgcacccga ccgacggccg tcagcgccgc
     3301 gtatgccggg cctggagcgg gatgcgtgat gaacatgcgc gaggggctct caacagggcg
     3361 ttgacggtaa gggacggggc cggccagccg gcgaatgatg gccgggtaat ggccggcgca
     3421 caggataacc gggccccgcc ggccggagtg tggactgctt ggcacaatgc gggcgaggca
     3481 accacaacgc gcaatccggc ggcgtgccac agatcacgcc gcgcggcgcc cgatcagtcg
     3541 ttgcccagcg ccagcagcgc ggcgttgccg cccaccgcgg cggtgttggt cgccgtgcag
     3601 cgctcgctga cgaagcgcag caggtagttc ggccccccgg ccttgggacc ggtgcccgac
     3661 agaccgagcc cgccgaatgg ctgcaccccc accaccgcac cgatctggtt gcggttgacg
     3721 tagaggttgc cgacccgcgc cagccgctcg atacgccgca cggtgtcgtc gttgcggctg
     3781 tgcacgccga gggtcaggcc gtagccggtg gcgttgatcg ccgcgaccac ttgctccagc
     3841 tcgccggcgg caaagcgcac cacgtgcagc accgggccga aatgctcccg ctcgagcgcg
     3901 tggatgccct cgatcgcgaa ggccaccggg gcgacgaagt ggccgtccag cccggcgggc
     3961 aacggcgtct cggcaatcag gcggccctcg cccttgagcc ggtcgatgtg cgccagcagc
     4021 gtcgcgcgcg cctcgccgtc gatcagcggg ccgacatcgg tggcgcgccg gtcgggcgga
     4081 ccgacccgca gctcggccat ggcgccggcg agcagctcga tcacccgcgg ggcgatatcc
     4141 tcctgcacgt agagcacgcg cagcgccgaa cagcgctggc cggcactggt gaaggcggac
     4201 tgcacggcat ccttgaccac ctgctccggc agagcggtgg agtcgacgat catcgcgttc
     4261 tgcccgccgg tctcggcgat cagcacagcg agggcgccgg gcttgtcggc cagctggcga
     4321 ttgatcagtc gcgcggtgtc ggtcgagccg gtgaagcaca ccccggcgag gcgcgcatcg
     4381 ctgcagaagg ccgcccccag cgtggcgccg tcgccgggca gcagcgccag caccgcgggc
     4441 ggcagaccgg cctccagcag cagctcgacg gcacgcatgg cgatcaggct ggcctgctcg
     4501 gccggcttgg ccagcacggt gttgccggcc accagggccg cggcaatctg gccgaggaag
     4561 atcgccagcg ggaagttcca cggactgatg caggcgaaca cgccgcgccc cgcgtagaac
     4621 agttcgttgc gctcgccggt cggccccggc agttcgtcgc gcagcagacg ctcgcgggcc
     4681 tgcgcggcgt agtaacggca gaagtccacc gcctcgcgca cctcgtcgat gccgtcctgc
     4741 agggtcttgc cggcctccag gcagcacagc gccagcagct cggcgcggtg cacctccagc
     4801 aggtaggcca ggcgctcgag caccgccgcg cgctcggcca ccggggtggc gctccagtgc
     4861 ggaaaggccg ccgccagacg gtccat
//
LOCUS       DATCUN010000298         2495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_14997, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000298 DATCUN010000000
VERSION     DATCUN010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14997"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VIO83_11575"
     CDS             <1..314
                     /locus_tag="VIO83_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8331951.1"
                     /translation="AKGGVNALTACLALETAERGIRVNATAPGGTEAPPRRIPRNSAE
                     QSEQEKVWYQQIVDQTIDSSLMKRYGTIDEQAGAILFLASDEASYITGVTLPVGGGDL
                     G"
     gene            396..1736
                     /locus_tag="VIO83_11580"
     CDS             396..1736
                     /locus_tag="VIO83_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020192425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8331952.1"
                     /translation="MKKIDVHDIIDNAPFTRFHWMVMCLCALLLIFDGYDLFIYGVVL
                     PVLMKEWGLTPLQAGALGSYALFGMMFGALCFGTLADKIGRKKGIAICFVLFSGATVL
                     NGFASGPTEFGILRFVAGLGCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYAVGGML
                     SAGVGMYMLPRFGWEAMFFAAAVPLLLLPVIMLFLPESIGFLVRQGRSEQARALLERV
                     DPARKLQADDTLEMVDGKGKGAAVLELFREGRAVRTLSIWVAFFCCLLMVYALSSWLP
                     KLMANAGYSLGSSLSFLLALNLGSVVGAIGGGWLGDRMNLPRVLVAFFIVASVSISLL
                     GFKTPMPVLYLLIAVAGATTIGTQILLYACAAQFYGLSIRSTGLGWASGIGRNGAIVG
                     PLLGGALLGINLPLQLNFMAFAIPGAIAALAMVVFSLSGRSAVQAQSLADRAAA"
     gene            2201..>2495
                     /locus_tag="VIO83_11585"
     CDS             2201..>2495
                     /locus_tag="VIO83_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family outer membrane porin"
                     /protein_id="HEY8331953.1"
                     /translation="MMASSGVALAFPVAAMAAEGGFIEDSTATLQLRNYYFSRDFSDI
                     VGNAAAQDKSKAEEWAQGFILNFKSGYTPGTVGFGVDAIGLLGVKLDSGKGRVG"
BASE COUNT          402 a          814 c          791 g          488 t
ORIGIN      
        1 cggccaaggg cggcgtcaac gcactgaccg cctgcctggc cctggaaacc gccgagcgcg
       61 gcatccgcgt caatgccacc gcaccgggcg gcaccgaggc gccaccgcgg cgcatcccgc
      121 gcaacagcgc cgagcagagc gagcaggaga aggtctggta ccagcagatc gtcgaccaga
      181 ccatcgacag cagcctgatg aaacgctacg gcaccatcga cgaacaggcc ggggcgatcc
      241 tcttcctcgc ttccgacgag gcctcctaca tcaccggcgt gaccctgccg gtaggtggtg
      301 gcgacctcgg ctgaaccatc catttgtctt cccgcccgct gcgcccatgc gcagcgggtt
      361 tttcctgcgc tgcaaataaa aacaagagat aagccatgaa aaaaatcgac gtccacgaca
      421 tcatcgacaa cgcgcccttc acccgtttcc actggatggt catgtgcctg tgcgcgctgc
      481 tgctgatctt cgacggctac gacctgttca tctacggcgt ggtcctgccg gtgctgatga
      541 aggagtgggg gctgaccccg ctgcaggccg gcgcgctggg cagctatgcg ctgttcggca
      601 tgatgttcgg cgccctgtgt ttcggcaccc tggccgacaa gatcggccgc aagaagggca
      661 tcgccatctg cttcgtgctg ttcagcggtg ccaccgtgct caacggcttc gccagcggcc
      721 cgaccgagtt cggcatcctg cgcttcgtcg ccggcctcgg ctgtggcggc ctgatgccca
      781 acgcggtggc gctgatgaac gagtacgcgc ccaagcgcct gcgcagcacc ctggtcgcca
      841 tcatgttcag cggctacgcg gtgggcggca tgctttccgc cggcgtcggc atgtacatgc
      901 tgccgcgctt cggctgggag gcgatgttct tcgccgccgc cgtgccgctg ctgctgctgc
      961 cggtgatcat gctgttcctg cccgagtcga tcggtttcct ggtccgccag gggcgcagcg
     1021 agcaggcccg tgcgctgctc gagcgcgtcg atccggcgcg caagctgcag gctgacgaca
     1081 cgctggaaat ggtcgacggc aagggcaagg gcgctgccgt gctggagctg ttccgcgaag
     1141 gtcgtgcggt gcgcaccctg tccatctggg tggcgttctt ctgctgcctg ctgatggtct
     1201 acgcgctcag ctcctggctg cccaagctga tggccaacgc cggctacagc ctgggctcca
     1261 gcctgtcgtt cctgctcgcc ctgaacctcg gttcggtcgt cggcgccatc ggcggtggct
     1321 ggctgggcga tcgcatgaac ctgccgcggg tgctagtggc gttcttcatc gtcgcttcgg
     1381 tgtcgatcag cctgctcggc ttcaagaccc cgatgccggt gctctacctg ctgatcgccg
     1441 tcgccggcgc caccaccatc ggtacgcaga tcctcctgta cgcctgtgcc gcgcagttct
     1501 acggcctgtc gatccgttcc accggcctcg gctgggcctc cggcatcggc cgcaacggcg
     1561 ccatcgtcgg cccgctgctg ggcggggcgc tgctcggcat caacctgccg ctgcaactca
     1621 acttcatggc cttcgccatc ccgggggcga ttgccgccct cgccatggtg gtgttctcgc
     1681 tgagcggccg cagcgccgtg caggcgcaga gcctggcgga tcgcgcggcg gcctgaggcc
     1741 gtcatcgcgg gctgccggac gcagcacggc gcgccactcg ggtgcaccgt actgcgtccg
     1801 gcaatatata cggcgcgggc gtattcctat gcccatgccg atctccggaa taattattca
     1861 gggctactga agttgttcgg ctgccgatat cacttgctgc gctgatggtt gtatttctat
     1921 cggggttgaa tgggtgtgcg ttcagcgaac acataaataa agttgcggtt gtgttctgca
     1981 gtggaaaata gaagatgggc aacaggcgcg gttgcgaaac atccatcaga tgacgcagca
     2041 gccatcgcct tcgagttttc cgcggtatgc aattaatggg ttgttgcctt gttcagccta
     2101 ttgctgaaag ggccttgctt ggccggtcgt cctgtggcgg cggtgtattc aaataataaa
     2161 aaagagtagg cacatgaaaa aagcgaatct tccctatctg atgatggctt ccagcggtgt
     2221 ggcgctggca tttccggtcg cggccatggc agccgagggc ggcttcatcg aggactccac
     2281 cgcgacccta cagctgcgca actactactt cagccgcgac ttctccgaca tcgtcggcaa
     2341 cgcggcggcg caggacaaat ccaaggccga ggagtgggcc cagggcttca tcctcaactt
     2401 caagtccggc tacaccccgg gcaccgtcgg cttcggcgtc gatgccatcg gcctgctcgg
     2461 ggtcaagctg gacagcggca agggccgggt cggca
//
LOCUS       DATCUN010000299         2425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_53078, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000299 DATCUN010000000
VERSION     DATCUN010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53078"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /gene="tssG"
                     /locus_tag="VIO83_11590"
     CDS             <1..905
                     /gene="tssG"
                     /locus_tag="VIO83_11590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEY8331954.1"
                     /translation="APVVPGAPVPRAGNNPAVPVVRLYGLGLLGPNGPLPLHYTEMVR
                     ERTENHNDSTLADFLDLFHHRYLTHMYRAWSQSQAAAGLDRADDETFSRYIAQLTGHD
                     PLEIRTSVLPPHARMAASTHLSREARNPDGLAQTLARFFAVPVRLDEFVMHWIRIDDE
                     DQTRLGKPRTSSVMGVGAIAGEVVADRQNKFRLVLGPLQLDQYLRFTPQGKDLPLLVA
                     WVRGFVGYEFVWEVELRVRNDSAPPARLGDVEKLGWSTWLGGSKSAGAMATAAATAPT
                     APPVDYAVGMVFEPEQYLRQSLGA"
     gene            940..1998
                     /locus_tag="VIO83_11595"
     CDS             940..1998
                     /locus_tag="VIO83_11595"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HEY8331955.1"
                     /translation="MNAAALPSRPNPSPPLSERPAIDWLQPVSALAPCGPSLEYDHEY
                     AVLLSRMTPQADAQYGSFVGTPEVPNWAEVERDCQRLLLRTKDINLLVWLCRARTRLG
                     QAAGLAHVLGMLRELLEAWPEAVHPQLIIDGEAEPAVRANALAGLVDPEGLLADVGEI
                     AVASNTAMRLTVRDVERAFAVPRLPEAATPESVTQQLAALRASPRAPMPPLAWLEVAA
                     GHVHAIDAWARAQLGDEAPALTPLSRMLDLFVDPSARHAAADPAETEAVMETPLPVPA
                     IEPIAPDRVQAVTRDDVLARIRSAREWFEHHEPSSPVAVLLKQAERMVGKRFSQVADS
                     IPLELLQKWEGDGEEVSA"
     gene            1995..>2425
                     /locus_tag="VIO83_11600"
     CDS             1995..>2425
                     /locus_tag="VIO83_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331956.1"
                     /translation="MNANLGRVLVALSCGLIGAWIGVLLVARQVRRRLARAPDAAAHA
                     PAHADAIHALALSLDARFAAQQRAWQETVERLPQTVQQALQVEFDFLAQQQAQRDLHR
                     TQREQERDEAMRVLIASLKARVPAPEARPAVPSPAPAPAARP"
BASE COUNT          361 a          882 c          820 g          362 t
ORIGIN      
        1 aggcgcccgt ggtgccgggc gcgcccgtgc cgcgcgccgg caacaacccg gcggtgccgg
       61 tggtgcgtct gtatggcctc ggcctgctcg ggcccaacgg ccccttgccg ctgcactaca
      121 cggagatggt gcgcgagcgc accgagaacc acaacgacag cacgctcgcc gacttcctcg
      181 acctctttca tcaccgctac ctcacgcaca tgtaccgtgc gtggtcgcag agccaggccg
      241 cagccggcct cgatcgcgcc gacgacgaaa ctttcagccg ctacatcgcg cagctgacgg
      301 ggcatgaccc gctggagatc cgcacgtcgg tgctgccgcc ccatgcacgc atggccgcct
      361 ccacgcacct gagccgcgaa gcgcgcaacc ccgacggcct ggcgcagacc ctggcgcgct
      421 tcttcgcggt gcccgtccgg ctggacgaat tcgtcatgca ctggatccgc atcgacgacg
      481 aagaccaaac ccgtctcggc aagccgcgca cctccagcgt catgggcgtc ggcgcgatcg
      541 cgggggaggt cgtggccgat cggcagaaca agttcaggct tgtgctcggc cccctgcaac
      601 tcgaccagta cctgcgcttc accccgcagg gcaaggacct gccgctgctg gtggcgtggg
      661 tgcgcggctt cgtcggctac gagttcgtct gggaggtcga actgcgggtg cgcaacgaca
      721 gcgcgccacc cgcgcgcctg ggcgatgtcg agaagctcgg ctggtcgaca tggctgggcg
      781 gctccaagtc ggcgggtgcg atggcaaccg cagcagcaac agcgcccacc gcgccgccgg
      841 tcgactatgc cgtcggcatg gtgttcgagc ccgagcaata cctccgccag tctctcggcg
      901 catgacgcgt cgtcttcttc attttctaga tccgtccgca tgaacgccgc cgctctgcct
      961 tcccgcccga acccatcgcc gcctctctcg gagcgccccg ccatcgactg gctccagccg
     1021 gtgagcgccc tcgcgccctg cgggcccagc ctggagtacg accacgaata cgccgtgctg
     1081 ctgtcgcgca tgacgcccca ggccgacgcc cagtacggca gcttcgtcgg caccccggaa
     1141 gtgcccaact gggccgaggt ggagcgcgac tgccaacgcc tgctgctgcg caccaaggac
     1201 atcaatctgc tggtctggct gtgccgcgcc cgcacccgcc ttggccaggc ggcggggctg
     1261 gcgcatgtgc tcggcatgct gcgcgaactg ctggaagcct ggcccgaggc ggtgcacccg
     1321 caactgatca tcgacggcga ggccgagccg gcggtccgtg ccaacgccct ggccggcctg
     1381 gtggaccccg agggcctgct ggccgacgtg ggggagatcg ccgtggcctc gaacaccgcc
     1441 atgcgcctga cggtacgcga cgtggagcgc gcctttgccg tgccccggtt gcccgaggca
     1501 gcgacgccgg aatccgtgac acagcagctc gcggccctgc gcgcgtcgcc cagggcgccc
     1561 atgccgccgc tggcctggct ggaagtggcg gcgggtcacg tgcatgcgat cgatgcatgg
     1621 gcccgggcgc aactcggcga cgaggcgccc gcgctgacgc cgttgagccg gatgctcgac
     1681 ctgttcgtgg acccatcggc caggcacgct gcggccgacc ccgccgagac ggaggcggtc
     1741 atggaaacac ccttgccggt tcccgccatc gaacccatcg cgccggatcg cgttcaggcc
     1801 gtcacgcgcg acgatgtgct cgcacgcatc cggagcgccc gcgaatggtt cgagcatcac
     1861 gagcccagca gcccggtggc ggtgttgttg aagcaggccg agcgcatggt gggtaagcgc
     1921 ttctcgcagg tcgcggattc gattccgctc gaactgctgc agaaatggga aggcgacggc
     1981 gaggaggtga gcgcatgaac gccaacctgg ggcgggttct ggtggctttg tcgtgcggct
     2041 tgatcggggc gtggatcggt gtcctgctgg tggcccgcca ggtccggcgg cgcctcgcgc
     2101 gcgcgcccga cgccgccgcc catgcaccgg cacatgccga tgcgatccat gccctcgcgc
     2161 tatcgctcga cgcccggttt gccgcccagc agcgcgcctg gcaggaaacg gtcgagcgcc
     2221 ttccgcagac cgtacaacag gcgttgcagg tcgaattcga cttcctggcg cagcaacagg
     2281 cgcagcgcga tctccaccga actcagcgtg aacaggagcg cgacgaggca atgcgcgtgc
     2341 tcatcgcttc gttgaaggcg cgcgtgccgg cgcctgaagc gcgaccggcc gtcccgtctc
     2401 cggcgcctgc cccggcggcg cgtcc
//
LOCUS       DATCUN010000300         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_128052, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000300 DATCUN010000000
VERSION     DATCUN010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128052"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VIO83_11605"
     CDS             complement(<1..261)
                     /locus_tag="VIO83_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY8331957.1"
                     /translation="MRRTKEEAEQTRRALLAAAEQLFLEQGVAHTTLEQIARAAGVTR
                     GALYWHFENKAHLFQAMLEQVRLPPEQLAERLSTCGDRDPLQV"
     gene            409..552
                     /locus_tag="VIO83_11610"
     CDS             409..552
                     /locus_tag="VIO83_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331958.1"
                     /translation="MPLQILLILSALTAFAPLPIDFGALRAAWRAARYQPDSVAGVVS
                     AKV"
     gene            complement(654..932)
                     /gene="rpsT"
                     /locus_tag="VIO83_11615"
     CDS             complement(654..932)
                     /gene="rpsT"
                     /locus_tag="VIO83_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY8331959.1"
                     /translation="MANSPSAKKRARQAEKRRSHNASLRSMVRTYIKNVVKAIDAKDL
                     AKAQVAYTAAVPVIDRLADKGIIHKNKAARHKSRLNAHIKALGAAAQA"
     gene            1094..>2289
                     /gene="murJ"
                     /locus_tag="VIO83_11620"
     CDS             1094..>2289
                     /gene="murJ"
                     /locus_tag="VIO83_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY8331960.1"
                     /translation="MSGESTKSGLLRSSAVVSVMTLLSRVLGMVRDMVVAAYFGSSPA
                     ADAFFIAFKIPNFMRRLFAEGAFAQAFVPVLSEYRAQRSLAEVKQLVDRVAGTLGLTL
                     TAITALGVVASPYLIMLFAPGFRGEPEKLALAGELLRITFPYLLLISLTAFCGGILNS
                     YGRFAVPAFTPVLLNVCMIGATVLLTPYFDQPIMALAWGVFIAGMAQLLFQLPFLARI
                     QLLPIPRPNRKDEGVKRIMTLMLPALFGVSVSQINLLLDTVLASFLQTGSVSWLYYAD
                     RLSELPLGAFGIAIGTVILPSLSRLHASGDPKAFSATIDWALRMVLLVGVPAALALAI
                     LSEPLIASLFHYGKMTENDVVQAANALEAYSLGVLTFMLIKVLAPAFFARQDLKTPVR
                     IAVQCML"
BASE COUNT          324 a          711 c          768 g          486 t
ORIGIN      
        1 gacctgcagc ggatcgcggt cgccgcaggt ggacaggcgc tcggccagct gctccggcgg
       61 caggcgcact tgctcgagca tggcctggaa caggtgggcc ttgttctcga agtgccagta
      121 gagcgcgccg cgggtcacgc cagcggcgcg ggcgatctgc tcgagggtgg tgtgggccac
      181 tccctgttcg agaaacagct gttcggcggc agccagcagg gcgcggcgtg tctgctcggc
      241 ttcttctttg gtgcggcgca tccggtacct cgggcgaaga accacacctc gggtgtgggg
      301 atcgcggatt attttgtcag gctaattaca ggcatgcctg aatgtatctg ggcgtgacgg
      361 ctttccgtgc atcgccacgg tcatcgccag tcctgttcgg gaggactgat gccgctgcaa
      421 atcctgctga tcctcagcgc cctcacggcg ttcgcgccgt tgccaatcga ttttggcgcg
      481 ttgcgtgcgg cctggcgtgc ggctcggtac cagccggata gcgtcgcagg agtggtgtcg
      541 gccaaggttt agagtagtgg ggcggtgtgc gcagtgcgct ccctgcggtg ggcagcactt
      601 ttctgcgggc aacaaaaaac cggccgaagc cggtttcttg tcggaacaga agcttaagcc
      661 tgagcggctg cgccgagcgc cttgatgtgg gcgttcagac ggctcttgtg acgagcagct
      721 ttgttcttgt ggatgatgcc tttgtcagcc aggcggtcga tcaccggtac agcagcggtg
      781 taggcgactt gagccttggc caggtctttg gcgtcgatag ccttgaccac gttcttgatg
      841 taggtacgca ccatcgagcg caggctggcg ttgtggctgc ggcgcttctc agcctgtctg
      901 gcgcgttttt tggcggaggg ggagtttgcc accgtcgagc tcctcgaatt ctgggggata
      961 ccaaataaat aaggccgcga atcatgccga ggcctgacgg tgttgtcaag cgccggcggc
     1021 ggctgcgggt tgctggtcgg acgggcaggc actggcttaa actcgccgac ctttgcaggg
     1081 tggggattaa agcatgtccg gtgagtcgac caaaagcggt ctgttgcgct ccagtgccgt
     1141 agtcagtgtg atgaccctgc tctcaagggt gctcggcatg gtccgcgaca tggtggtggc
     1201 ggcctatttc ggctcgagcc cggcggcgga tgcgttcttc atcgccttca agattcccaa
     1261 cttcatgcgc cggctgttcg ccgagggcgc cttcgcccag gccttcgtcc cggtgctgtc
     1321 ggagtaccgc gcccagcgca gcctggccga ggtcaagcag ctggtcgacc gggtcgccgg
     1381 caccctcggg ctgaccctga ctgcgatcac cgccctcggc gtggtggcct cgccctacct
     1441 gatcatgctg ttcgcgccgg gctttcgcgg cgagccggaa aagctcgccc tggccggcga
     1501 gctgctgcgc atcacctttc cctacctgct gctgatctcg ctgaccgcct tctgcggcgg
     1561 cattctcaac agctacgggc gcttcgccgt gccggccttc acgccggtgc tgctcaacgt
     1621 ctgcatgatc ggcgcgaccg tgctgctcac cccgtatttc gaccagccga tcatggcgct
     1681 ggcctggggc gtgttcatcg ccggcatggc ccagctgctg ttccagctgc cgtttcttgc
     1741 gcgcatccag ctgctgccga tcccgcggcc gaaccgcaag gacgagggcg tcaagcggat
     1801 catgacgctg atgctgccgg cgctgttcgg cgtgtcggtc agccagatca acctgctgct
     1861 ggataccgtg ctggcgtcct tcctgcagac cggcagcgtg tcctggctgt actacgctga
     1921 ccgcctgtcg gaattgccgc tgggcgcctt cggcattgcc atcggcacgg tgatcctgcc
     1981 cagcctgtcg cgtctgcatg ccagcggcga tcccaaggcc ttctcggcga ccatcgactg
     2041 ggcgctgcgc atggtgctat tggtcggcgt gccggcggcg ctggccctgg cgatcctttc
     2101 cgagccgctg atcgccagcc tgttccacta cggcaagatg accgagaacg acgtggtgca
     2161 ggcggcgaat gcgctggagg cctattcgct gggcgtgctc accttcatgc tgatcaaggt
     2221 gctggcgccg gcattctttg cccgccagga tctgaagacc ccggtgcgta tcgccgtgca
     2281 gtgcatgct
//
LOCUS       DATCUN010000301         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_165837, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000301 DATCUN010000000
VERSION     DATCUN010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165837"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VIO83_11625"
     CDS             complement(<1..453)
                     /locus_tag="VIO83_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8331961.1"
                     /translation="MNAMLTVLRRELAAYFYTPLAYVFIVIFLFLNGIFTFYLGQFFS
                     RGQADLLPFFQFHPYLYLVLIPPVAMRLWAEERKTGTLELLMTLPITTGQAVVGKFLA
                     AWLFTAVALALTFPIWITVSYLGDPDHGAIVAGYLGSLLMAGAYLAIGA"
     gene            complement(450..1412)
                     /locus_tag="VIO83_11630"
     CDS             complement(450..1412)
                     /locus_tag="VIO83_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8331962.1"
                     /translation="MQPGVAVRHLTKDFGGLRAVDDLSFSVGGGEVVGFLGPNGAGKS
                     TTMKMITGYLAPSSGQVEVCGMDLAQQPLAIKAAIGYLPEGAPLYGDMTPRRFLDFVA
                     SVRGMEPAVRRQRIDAVVDRMALRGVLDQSIDTLSKGFKRRVGLAQAILHDPPVLILD
                     EPTDGLDPNQKHEVRELIRDIAADKVIIISTHILEEVEAICSRAVIIAGGRLLADGRP
                     AELASRSQFHNAVVLVLSGGPADLREQLGAVPGVQRVEVLEESAGRVRLRLLASHGRS
                     LLEAVSARLHEAGWQADELYVEQGRLDDVFRQLTHEVAGRETRS"
     gene            1782..>2010
                     /locus_tag="VIO83_11635"
     CDS             1782..>2010
                     /locus_tag="VIO83_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY8331963.1"
                     /translation="MTQAFTAAVVQMVSGSEVATNLASADRLIAQAAQAGARLVALPE
                     NFALLGRREQDKLAVAEADGGGPIQDFLADSA"
BASE COUNT          350 a          733 c          635 g          292 t
ORIGIN      
        1 ggcgccgatc gccaggtacg cgccggccat cagcaggctg cccagatatc cggccacgat
       61 ggcgccatgg tccggatcgc cgaggtaact gaccgtgatc cagatcggga aggtcagcgc
      121 cagcgccacc gccgtgaaca gccaggcggc caggaacttg ccgaccaccg cctggccggt
      181 ggtgatcggc agcgtcatca gcagctccag cgtgccggtc ttgcgctcct ccgcccacag
      241 gcgcatcgcc accggcggga tcagcaccag gtacaggtac ggatgaaact ggaagaacgg
      301 cagcaggtcg gcctgcccgc ggctgaagaa ctggcccaga taaaacgtga atatcccgtt
      361 caggaacagg aagatcacga tgaacacata ggccagcggg gtatagaaat acgccgccag
      421 ctcgcgccgc agcaccgtca gcatggcatt catgagcgcg tctcccggcc ggccacctcg
      481 tgcgtcagct gccggaacac atcgtccagg cgcccctgtt ccacatacag ctcgtcggcc
      541 tgccagccgg cctcatgcag cctggcgctg accgcctcca gaaggcttcg gccgtggctg
      601 gccagcaggc gcaggcggac ccgcccggcg gattcttcca atacctccac ccgctgcacg
      661 cccggcacgg cgcccagttg ctcacgcagg tcggcaggac cgccggacag caccagcacc
      721 accgcgttgt ggaactgcga gcgactggcc agttccgccg gccggccgtc ggccagcagg
      781 cgcccgccgg caatgatcac ggcgcggctg cagatggcct ccacctcttc caggatgtgg
      841 gtggagatga tgatcacctt gtcggcggcg atgtcgcgga tcagctcgcg cacttcgtgc
      901 ttttgattgg ggtccaggcc gtcggtcggc tcgtccagga tcaacaccgg cgggtcgtgc
      961 aggatggcct gcgccagacc cacgcggcgc ttgaagccct tcgacagggt gtcgatggac
     1021 tggtccagca cgccgcgcag cgccatgcgg tccaccaccg cgtcgatgcg ctggcgccgc
     1081 acggccggct ccatgccgcg cacgctggcg acgaaatcca gaaaccgccg cggcgtcatg
     1141 tcaccgtaca gcggcgcgcc ctccggcagg tagccgatgg cggccttgat cgccagcggc
     1201 tgctgggcca gatccatgcc gcacacctcg acctggccgc ttgaaggcgc caggtagccg
     1261 gtgatcattt tcatggtggt ggacttgccg gcgccgtttg gccccagaaa ccccaccacc
     1321 tcgccgccgc cgacgctgaa cgacagatcg tccaccgccc gcaggccacc gaaatccttg
     1381 gtcagatgcc tgaccgcgac ccctggctgc atgttctcct cgctttccaa atcctgccca
     1441 ccgcgtcggc aggacaaaag gcccggcatt ctagccacat gctcgaatcg gcgccgcgcc
     1501 ggcccagttg gcccaagggc gggcctccca caaataacgg atcgtaggag cggcgccccc
     1561 gccgcgatct ccccggatac cgcaccccca ccccatcggc ccgggggcgg gcctcctaca
     1621 ggcgtctgtg taggagcggc gccctcgccg cgattcttca atgccgaccc ttccaggcca
     1681 acccttcggc ccgagggcgg gcctcccaca gggtggcgaa tcttcagcca cctttcccta
     1741 ccataggcgc gattcgacca cgacagaccg aggcgagttt catgacccag gcattcacgg
     1801 cggcagtcgt gcagatggtg tccggcagcg aggtagcgac caatctggcc agtgccgacc
     1861 ggctgatcgc ccaggccgcg caggccggcg cccggctggt ggcgctgccg gagaacttcg
     1921 ccctgctcgg ccggcgcgag caggacaaac tggccgtggc cgaagccgac ggcggcgggc
     1981 cgatccagga tttcctggcc gacagcgcac
//
LOCUS       DATCUN010000302         8167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_89698, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000302 DATCUN010000000
VERSION     DATCUN010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89698"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VIO83_11640"
     CDS             <1..454
                     /locus_tag="VIO83_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017478245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY8331964.1"
                     /translation="QPVQAVAPASTAPKPAAVVAPVVEAAPKAAAVAVKPAAAAPVAS
                     APAPAAGGTGNSSWYLSQPVGQYTLQLLGTASEGAAQSMVRDGGGEYRYFRKLHQGRP
                     LYVVTYGRFSSPEAAKSAVSALPSRLQAGKPWPRTFASIQQEIRQAGR"
     gene            690..5135
                     /gene="gltB"
                     /locus_tag="VIO83_11645"
     CDS             690..5135
                     /gene="gltB"
                     /locus_tag="VIO83_11645"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEY8331965.1"
                     /translation="MKAGLYHPEQFKDNCGFGLIAHMHGQPSHHLLQTAIEALTCMTH
                     RGGINADGKTGDGCGLLLQKPDRFLRVLAAEQFGVHLPEQYAVGMVFLSQDETKAASA
                     REAVNAQLAAQGLSLVGWRAVPVDTSVLGRLAQECLPRIEQVFVSGEGLDDQQMAIKL
                     FYARRHAEVALKDDADFYVCSLSAKDIIYKGLMMPADLASFFPDLGDERLETAICVFH
                     QRFSTNTLPRWPLAQPFRFLAHNGEINTITGNRNWAVARRNKFANELLPDLESIAPLV
                     NRTGSDSSSMDNMLELMVTGGMDLFRGLRMLIPPAWQNVETMDVELRSFYEYNSMHME
                     PWDGPAGVVLTDGRHAVCLLDRNGLRPARWVTTKNGYITLASEVGVWNYAPEDVLAKG
                     RVGPGQILAVDTETGQVLHTADIDNRLKSRHPYRQWLRQNALRIQAGMDDDHGGATYD
                     AEQLKRYMKMFQVTFEERDQVLRPLAEQGQEAVGSMGDDTPMAVLSRRVRSNYDYFRQ
                     QFAQVTNPPIDPLREAIVMSLETCLGAERNVFEESADHANRAILNTPVISPAKWQTLM
                     TLDRPGFERQIIDLNYDESVGLEAAVRAIADQAEAAVRAGKVLLVLSDRHIAPGKLPV
                     HASLAVGAVHHRLTEKGLRCDCNILVETATARDPHHFAVLIGFGATAVYPFLAYEVLA
                     DLIRTGEVLGDLHESFKHYRKGISKGLLKILSKMGISTVASYRGAQLFEAIGLADEVV
                     ELSFRGVASRIQGARFVDLEAEQKLLAAEAWNNRKAIQQGGLLKFVHGGEYHAYNPDV
                     VRLLQEAVQQGDYARFRDYTALVDSRPVSMLRDLLKVKEAAQPLALDQVEPLNAIFKR
                     FDAAGISLGALSPEAHEALAEAMNRLGGRSNSGEGGEDPARYGTIKSSKIKQVATGRF
                     GVTPEYLVNAEVLQIKVAQGAKPGEGGQLPGGKVNGLIARLRYAVPGVTLISPPPHHD
                     IYSIEDLAQLIFDLKQVNPQALVSVKLVAEPGVGTIAAGVAKAYADLITISGYDGGTG
                     ASPLTSIKYAGSPWELGLAEAHQTLRGNDLRGKVRVQTDGGLKTGLDVIKAAILGAES
                     FGFGTAPMIALGCKYLRICHLNNCATGVATQNDQLRKDHFIGTVEMVMNFFTFIAEET
                     REWLAKLGVRSLGELIGRTDLLEMLPGETAKQGNLDLSPLLGSAHIPADKPQFCEVEK
                     NPPFDQGLLAEKMVEMALPAIKAKRGGEFELDICNCDRSIGARISGEIARVHGNQGMV
                     DAPISFRFKGTAGQSFGVWNAGGLNLRLEGDANDYVGKGMTGGKIVITPPQGSALQSQ
                     ESAIIGNTCLYGATGGKLFAAGTAGERFAVRNSGAHTVVEGTGDHCCEYMTGGFVCVL
                     GKTGGNFGSGMTGGFAYVLDDDNSFFDRVNHELVDLQRISGEAMEAYRSHLRRVLVEY
                     VEETGSAWGRQILDNLDDYARRFWLVKPKAASLKSLLSSTRANPQ"
     gene            5243..6661
                     /locus_tag="VIO83_11650"
     CDS             5243..6661
                     /locus_tag="VIO83_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8331966.1"
                     /translation="MTERLNNDFQFIEVGRKDPKKKLLRQRKKEFVEIYDLFKPQQAT
                     EQAHRCLGCGNPYCEWKCPVHNYIPNWLKLVSEGNILAAAELSHQTNTLPEVCGRVCP
                     QDRLCEGACTLADGFGAVTIGSVEKYITDTAFAMGWRPDMSKVVPTGKRVAVIGAGPA
                     GLGCADVLVRNGVTPVVFDKNPEIGGLLTFGIPEFKLEKSVLTRRREVFSGMGIEFRL
                     NTEVGKDVTMDQLLAEYDAVFMGMGTYSYMKGGFAGEDLPGVYDALDFLIANVNRNLG
                     FEKSADDFIDMAGKKVVVLGGGDTAMDCNRTSIRQGAKAVTCAYRRDEENMPGSRREV
                     KNAKDEGVKFLFNRQPIAIVGEGRVEGVKVIETRLGEPDARGRRSPEPIPGSEEILPA
                     DAVVIAFGFRPSPAPWFEQFDIAIDGQGRVVAPEQAQYKFQTSNPKIFAGGDMVRGSD
                     LVVTAIFEGRQAAEGILDYLGV"
     gene            6774..7838
                     /gene="hemE"
                     /locus_tag="VIO83_11655"
     CDS             6774..7838
                     /gene="hemE"
                     /locus_tag="VIO83_11655"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY8331967.1"
                     /translation="MTVLKNDRFLRALLKQPVDVTPVWMMRQAGRYLPEYRASRAKAG
                     DFMSLCMNPELACEVTLQPLDRYPLDAAILFSDILTIPDAMGLGLYFETGEGPRFKKV
                     ISTPADIAALPIPDPEKDLGYVMDAVRTIRRELNGRVPLIGFSGSPWTLATYMVEGGS
                     SKDFRKSKAMLYDNPQAMHALLDKLAQSVTSYLNGQILAGAQAVQIFDTWGGSLSDAA
                     YREFSLFYMERIVAGLIRQHEGREVPVILFTKNGGQWLEAMADTGATALGLDWTCDIR
                     NARARVGDKVALQGNMDPTVLYANPTAIRTEVARILERFGQGSGHVFNLGHGITPEVD
                     PAHAGVFIEAVHELSAQYHQ"
     gene            complement(7939..>8167)
                     /locus_tag="VIO83_11660"
     CDS             complement(7939..>8167)
                     /locus_tag="VIO83_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8331968.1"
                     /translation="ASSWVGRRALQARGQASSLYLFSYYLGSSTAGTLGGFFWQHGGW
                     TGVGVFIAALLAAALLVALHLSRLPPKQAEA"
BASE COUNT         1459 a         2800 c         2640 g         1268 t
ORIGIN      
        1 gcagcctgtc caggcagtgg cacccgcgtc gacggcgcct aagccggcag cagtggtggc
       61 accggttgtc gaagcagcgc ccaaggcggc tgcggtggcg gtgaagccgg cagcggctgc
      121 gccagtggcc agcgctccgg cgcctgccgc cggcggcacc ggcaacagca gctggtatct
      181 gtcgcagccc gtcggccagt acaccctgca gttgctcggt acggcgtcgg aaggcgcggc
      241 acagagcatg gtgcgcgatg gcggtggcga gtaccgctac ttccgcaagc tgcaccaggg
      301 caggccgctg tacgtggtca cctatggccg cttctccagc ccggaggcgg ccaagtcggc
      361 ggtaagcgcc ttgccgagcc gtttgcaggc cggcaagccg tggccgcgga ccttcgccag
      421 catccagcag gaaatccgcc aggccggtcg ctaaactcga cgggtggggg cgaaaaaacc
      481 ggtgatgcac ctaggcgcat caccggtttt tcttttataa atgcgacatg aaaatttcgc
      541 tttcgcgcca tttgttcttt ttcggacata agtcgcacgc tacaatggcg catccgtgat
      601 cgcagcaagc gccgcaaaag tgttggcaag gcgcgataag ttgcgaaaaa acgcccgaat
      661 cgaatagaag ttagccggtg agagtgccta tgaaagctgg tctgtaccat cccgagcagt
      721 tcaaggataa ctgcggcttc ggtctgattg cccacatgca tgggcagccc agtcaccacc
      781 tgctgcagac cgccattgaa gccctgacat gcatgaccca ccgcggcggc atcaacgccg
      841 acggcaagac cggcgacggc tgtggtctgc tactgcagaa gcccgatcgc ttcctgcgcg
      901 tgctggccgc cgagcagttc ggcgtgcacc tgccggagca gtatgccgtg ggcatggtct
      961 tcctcagcca ggacgagacc aaggctgcca gcgcccgcga ggcggtcaac gcccagctgg
     1021 cggcgcaggg cctgagcctg gtgggctggc gcgctgtgcc ggtggacacc tcggtgctcg
     1081 gccgcctcgc tcaggagtgc ctgccgcgca tcgagcaggt gttcgtcagc ggcgaaggcc
     1141 tcgacgacca gcagatggcg atcaagctgt tctatgcccg ccgccacgcc gaagtcgccc
     1201 tgaaggacga cgcggacttc tacgtctgca gcctgtcggc caaggacatc atctacaagg
     1261 gtctgatgat gccggccgac ctggccagct tcttccccga tctgggtgac gagcgcttgg
     1321 aaaccgcgat ctgcgtgttc caccagcgct tctccaccaa caccctgccg cgctggccgc
     1381 tggcccagcc gttccgcttc ctggcgcaca acggcgagat caacaccatc accggcaacc
     1441 gcaactgggc ggtggcgcgg cgcaacaagt tcgccaacga gctgctgccc gatctggaaa
     1501 gcatcgcgcc gctggtcaac cgcaccggtt ccgactcctc gagcatggac aacatgctcg
     1561 agctgatggt caccggcggc atggacctgt tccgcggcct gcgcatgctc attccgccgg
     1621 cctggcagaa cgtcgagacc atggacgtcg agctgcgcag cttctacgaa tacaactcca
     1681 tgcacatgga gccgtgggac ggtccggccg gcgtggtgct gaccgacggt cgccacgccg
     1741 tctgcctgct cgaccgcaac ggtctgcgcc cggcgcgctg ggtcaccacc aagaacggct
     1801 acatcaccct ggcctcggaa gtcggcgtgt ggaactacgc gccggaagac gtgctggcca
     1861 agggccgcgt cggtccgggg cagatcctcg ctgtggacac cgagaccggc caggtgctgc
     1921 acactgccga catcgacaac cgcctgaagt cgcggcatcc gtaccgccag tggctgcgcc
     1981 agaatgccct gcgcatccag gccgggatgg acgacgatca cggtggcgcg acctacgacg
     2041 ccgagcagct caagcgctac atgaaaatgt tccaggtcac cttcgaggag cgtgaccagg
     2101 tgctgcgccc gctcgccgag cagggccagg aagcggtcgg ctctatgggc gacgacaccc
     2161 cgatggcggt gctgtcgcgg cgcgtgcgct cgaactacga ctacttccgt cagcagttcg
     2221 cccaggtgac caacccgccg atcgacccgc tgcgcgaagc catcgtcatg tcgctggaaa
     2281 cctgcctggg tgccgagcgc aacgtgttcg aagagtccgc cgatcacgcc aaccgcgcga
     2341 tcctcaacac cccggtgatc tccccggcca agtggcagac gctgatgacc ctggaccgcc
     2401 cgggcttcga gcggcagatc atcgacctca actacgacga gtccgtgggc ctcgaggccg
     2461 ccgtgcgcgc catcgccgac caggccgagg ctgccgtgcg cgccggcaag gtgctgctgg
     2521 tgctgtccga ccgccacatc gcccccggca agctgccggt gcatgcctcg ctggccgtgg
     2581 gtgccgtgca ccaccgtctg accgagaagg gcctgcgctg cgattgcaac atcttggtgg
     2641 aaaccgccac cgcccgcgat ccgcaccact tcgccgtgct gatcggcttc ggtgccaccg
     2701 cggtctaccc gttcctcgcc tatgaggtac tggccgacct gatccgcacc ggcgaggtgc
     2761 tcggcgacct gcacgagtcg ttcaagcact accgcaaggg catctccaag ggcctgctga
     2821 agatcctctc gaagatgggc atctccaccg tcgcttccta ccgcggtgcg cagttgttcg
     2881 aagccatcgg cctggccgac gaggtggtcg agctgagctt ccgtggcgtc gccagccgca
     2941 tccagggcgc gcgcttcgtc gacctcgagg ccgagcagaa gctgctggcc gccgaggcgt
     3001 ggaacaaccg caaggcgatc cagcagggcg gcctgctcaa gttcgtccac ggcggcgaat
     3061 accatgccta caacccggac gtggtgcgtc tgctccagga agccgtgcag cagggcgact
     3121 acgcgcgctt ccgcgactac accgcgctgg tcgacagccg tccggtgtcg atgctccgtg
     3181 acctgctcaa ggtcaaggaa gcggcccagc cgctggcgct ggatcaggtc gagccgctca
     3241 acgccatctt caagcgtttc gacgccgccg gcatctccct cggcgcgctg tcgccggagg
     3301 cccacgaggc gctggccgag gcgatgaacc gtctgggcgg gcgctccaac tccggcgagg
     3361 gcggcgaaga cccggcccgc tacggcacca tcaagagctc gaagatcaag caggtcgcca
     3421 ccggccgctt cggcgtcacc ccggaatacc tggtcaacgc cgaagtgctg cagatcaagg
     3481 tcgcccaggg cgccaagccc ggcgagggtg gccagctgcc cggcggcaag gtcaacggcc
     3541 tgatcgcccg cctgcgctac gcggtgcctg gcgtgacctt gatctcgccg ccgccgcacc
     3601 acgacatcta ctcgatcgag gacctggccc agctgatctt cgacctcaag caggtcaacc
     3661 cgcaggcgct ggtgtcggtc aagctggtcg ccgagccggg cgtcggcacc atcgccgccg
     3721 gcgtggccaa ggcctacgcc gacctgatca ccatctccgg ctacgacggc ggcaccggcg
     3781 cttcgccgct gacctcgatc aagtacgccg gcagcccgtg ggagctgggc ctcgccgagg
     3841 cgcaccagac cctgcgcggc aacgacctgc gcggcaaggt ccgcgtgcag accgacggcg
     3901 gcctgaagac cggcctcgac gtgatcaagg ccgccatcct cggcgccgaa agcttcggct
     3961 tcggcaccgc gccgatgatc gccctgggct gcaagtacct gcgcatctgc cacctgaaca
     4021 actgcgccac cggcgtggcc acccagaacg accagctgcg caaggaccac ttcatcggca
     4081 ccgtcgagat ggtgatgaac ttcttcacct tcatcgccga ggaaacccgc gagtggctgg
     4141 ccaagctggg cgtgcgcagc ctgggcgagc tgatcgggcg taccgacctg ctggaaatgc
     4201 tcccgggcga gaccgccaag cagggcaacc tggacctcag cccgctgctc ggcagcgcgc
     4261 acattccggc cgacaagccg caattctgcg aggtggagaa gaacccgccg ttcgaccagg
     4321 gcctgctggc cgagaagatg gtcgagatgg cgctgccggc gatcaaggcc aagcgtggtg
     4381 gcgagttcga gctggacatc tgcaactgcg accgctccat cggtgcgcgc atctccggcg
     4441 aaatcgcccg ggtgcacggc aaccagggca tggtcgacgc gccgatcagc ttccgcttca
     4501 agggcaccgc ggggcagagc ttcggcgtgt ggaacgccgg cggcctcaac ctgcgcctgg
     4561 aaggcgacgc caacgactac gtcggcaagg gcatgaccgg cggcaagatc gtcatcaccc
     4621 cgccgcaggg cagtgcgctg cagagccagg aaagtgccat catcggcaac acctgcctgt
     4681 acggcgccac cggcggcaag ctgttcgccg cgggtaccgc gggcgagcgc ttcgcggtgc
     4741 gcaactccgg tgcccacacc gtggtggaag gcaccggcga tcactgctgc gaatacatga
     4801 ccggcggttt cgtctgcgtg ctgggcaaga ccggcggcaa cttcggctcc ggcatgacgg
     4861 gcggcttcgc ctacgtgctg gacgatgaca acagcttctt cgaccgcgtc aaccacgagc
     4921 tggtcgacct gcagcgcatc agcggcgagg ccatggaggc ctatcgcagc cacctgcgtc
     4981 gcgtgctggt cgagtacgta gaagagaccg gcagtgcctg gggtcgccag atcctcgaca
     5041 accttgacga ctacgcgcgc cgcttctggc tggtcaagcc gaaggccgcg agcctcaagt
     5101 cgctgctgtc cagcacccgg gccaacccgc agtaaggccg tgtcgaaccg caggtcgtgt
     5161 ccagcgaccg gcctgtcggt tccccagatg agtttcgcag cttgaagcac gtgacttgag
     5221 gctgcagcaa agaggttttg atatgactga acgtctgaac aacgacttcc agttcatcga
     5281 ggtcggccgc aaggatccga agaagaaact gctgcgccag cgcaagaagg aattcgtcga
     5341 gatctacgac ctgttcaagc cgcagcaggc gaccgaacag gcgcaccgct gcctgggttg
     5401 cggcaacccc tattgcgaat ggaagtgccc ggtgcacaac tacattccca actggctgaa
     5461 gctggtgtcc gagggcaaca tcctcgccgc cgccgaactg tcgcaccaga ccaacaccct
     5521 gccggaagtc tgcggccgcg tctgcccgca ggaccgcctg tgcgagggtg cctgcaccct
     5581 ggccgacggc ttcggcgcgg tgaccatcgg ctcggtggag aagtacatca ccgacaccgc
     5641 cttcgccatg ggctggcgtc cggacatgtc caaggtggtg ccgaccggca agcgcgttgc
     5701 ggtgatcggc gccggcccgg cgggtctcgg ctgcgccgac gtgctggtgc gcaacggcgt
     5761 gaccccggtg gtgttcgaca agaacccgga aatcggtggt ctgctgacct tcggcatccc
     5821 cgagttcaag ctggaaaaga gcgtgctgac ccgccgccgt gaagtgttca gcggcatggg
     5881 catcgagttc cgcctcaaca ccgaggtggg caaggacgtc accatggacc agctgctcgc
     5941 cgagtacgac gcggtgttca tgggcatggg cacctacagc tacatgaagg gcggcttcgc
     6001 cggcgaggac ctgccgggcg tctacgacgc gctggacttc ctgatcgcca acgtcaaccg
     6061 caacctcggc ttcgagaagt ccgccgacga cttcatcgac atggccggca agaaggtggt
     6121 ggtgctcggc ggtggcgaca ccgcgatgga ctgcaaccgc acctcgatcc gccagggcgc
     6181 caaggcagtg acctgcgcct atcgccgcga cgaggaaaac atgccgggtt cgcgccgcga
     6241 ggtgaagaac gccaaggacg agggggtgaa gttcctgttc aaccgccagc cgatcgccat
     6301 cgtcggcgaa ggccgcgtgg aaggcgtcaa ggtgatcgag acccgtctcg gcgagccgga
     6361 tgcccgtggc cgtcgcagcc ccgagccgat cccgggctcc gaggagatcc tgccggccga
     6421 cgcggtggtg atcgccttcg gcttccggcc gagcccggcg ccctggtttg agcagttcga
     6481 catcgccatc gatggccagg gccgcgtggt cgctccggag caggcgcagt acaagttcca
     6541 gaccagcaat ccgaagatct tcgccggcgg cgacatggtg cgcggctccg acctggtggt
     6601 caccgcgatc ttcgaaggcc gtcaggccgc cgaaggcatc ctcgactacc tgggcgtctg
     6661 atccccgccg gggctgaccc cggtcaagcg cgacacgaaa ggcacggcac ccgccgtgcc
     6721 ttttgcgttg tgctttgcga caatgcccgc ctgaattttc gccggaagcc gttatgaccg
     6781 tcctgaagaa cgaccgtttc cttcgcgccc tgctcaagca gcccgtcgac gtcaccccgg
     6841 tgtggatgat gcgccaggcc ggacgctacc tccccgagta ccgtgccagc cgcgccaagg
     6901 ccggtgactt catgagcctg tgcatgaatc ccgagctggc ctgcgaggtc accctgcagc
     6961 cgctggaccg ctatccgctg gatgccgcca tcctgttctc cgacatcctg accatcccgg
     7021 atgccatggg tctgggcctg tacttcgaga ccggcgaagg cccgcgtttc aagaaggtca
     7081 tcagcacccc ggccgacatc gccgcgctgc cgattcccga tccggaaaag gacctcggct
     7141 acgtgatgga tgcggtgcgc accatccgcc gcgagctcaa cggccgcgtg ccgctgatcg
     7201 gtttctccgg cagcccctgg accctggcca cctacatggt cgagggcgga tcaagcaagg
     7261 acttccgcaa gtccaaggcg atgctctacg acaacccgca ggccatgcac gcgctgctcg
     7321 acaagctggc ccagtcggtc accagctacc tcaacgggca gatcctcgcc ggcgcccagg
     7381 ccgtgcagat cttcgacacc tggggcggca gcctgtcgga tgcggcctac cgcgagttct
     7441 cgctgttcta catggaacgc atcgtcgccg gcctgatccg ccagcacgaa ggccgcgaag
     7501 tgccggtgat cctgttcacc aagaacggcg gccagtggct ggaggcgatg gccgacaccg
     7561 gcgccaccgc cctcggcctg gactggacct gcgacatccg caacgcccgc gcccgcgtcg
     7621 gcgacaaggt ggcgctgcag ggcaacatgg acccgaccgt gctgtacgcc aacccgacgg
     7681 cgatccgcac cgaagtggcg cgcatcctcg agcgtttcgg ccagggcagc ggccacgtgt
     7741 tcaacctcgg ccacggcatc accccggagg tcgatccggc ccacgccggg gtgttcatcg
     7801 aggcggtgca cgagctgtcg gcgcagtatc accagtgatc cgcgcgctgt tctgaaagac
     7861 aaaagcccga tcaatcgatc gggcttttgt ctttcagagg gtggaaaaga cctgcgctcg
     7921 ttttccaccc cggtgcgctc aggcctccgc ctgcttgggc ggcagccgcg acaggtgcaa
     7981 cgccaccagc agcgctgccg cgagcagtgc ggcgatgaac acgccgacac cggtccagcc
     8041 gccgtgctgc cagaagaagc cgccgagggt gccggcggtg ctggagccga ggtagtagct
     8101 gaacaggtac agcgacgagg cttggccgcg ggcctgcagg gcgcgacggc cgacccagct
     8161 gctggcc
//
LOCUS       DATCUN010000303         3245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_15338, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000303 DATCUN010000000
VERSION     DATCUN010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15338"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VIO83_11665"
     CDS             <1..264
                     /locus_tag="VIO83_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY8331969.1"
                     /translation="IGFGSTAVITGDVTQVDLPRGTKSGLAHVVEVLRDVPGISFTHF
                     KAKDVVRHPLVQRIVEAYDRYEHRLQGKPDKLNDTARDGRDAD"
     gene            254..733
                     /gene="ybeY"
                     /locus_tag="VIO83_11670"
     CDS             254..733
                     /gene="ybeY"
                     /locus_tag="VIO83_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY8331970.1"
                     /translation="MQIELDLQLATEAGELPDEAQLRRWCELALRQRTAPSELTIRIV
                     DEAEGRELNHTWRGKDYATNVLSFPAEVPEGPGGVPLLDIPLLGDLVICAQVVAREAG
                     EQGKSLDAHWAHLTIHGCLHLLGYDHIDDDEAEEMEALERQLLAELGYPDPYACDEE"
     gene            740..1579
                     /locus_tag="VIO83_11675"
     CDS             740..1579
                     /locus_tag="VIO83_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEY8331971.1"
                     /translation="MSEDRSSQGQRSWLERITQAFSHEPKSRQELLEVLREAHENKLL
                     DTEALAIVEGAIQVADLQVRDIMIPRSQIITIKACQSPQEFLPAVIDAAHSRYPVIGE
                     NLDEVVGILLAKDLLPLLLRDSTQAFDLHKLMRPATFVPESKRLNVLLREFRANRNHM
                     AIVIDEYGGVAGLVTIEDVLEQIVGDIEDEHDVEEDSYIKALPSGDFIVKALTPVEDF
                     NESFATTFAEDDFDTIGGVVMSTFGHMPKRNEVTDIGGFRFRVVNADSRRIHLLRVTP
                     LVR"
     gene            1752..>3245
                     /gene="lnt"
                     /locus_tag="VIO83_11680"
     CDS             1752..>3245
                     /gene="lnt"
                     /locus_tag="VIO83_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY8331972.1"
                     /translation="MRWITRPGWPGHLLALAAGALTPLALAPFDLWPLGLLSLALFYL
                     GLRGLAPRAALARGWCYGFGAFAAGTSWVYVSIHDYGAASPALAGLLTLLFSAGLALF
                     FALPAWVWVKWLRRERAPLADALAFAALWLAQEALRGWLFTGFPWLYIGYSQLAGPLA
                     GLAPLGGVWLLSFGMALTAALLANLPALLPRWSSALIALLLLVATWGSGLLLRDHAWT
                     QPAGAPLKVAAMQGNVEQSMKWDPEQLNAQLALYRDLTFAEAPADLYVWPETAVPVLK
                     EYAEGYLSVMSRFASERNAALITGVPLRQADGEGQPRYYNAVQVIGQGEGTYLKQKLV
                     PFGEYVPLQDVLRGLIAFFDLPMSDFARGPADQPMLQARGYRIAPYICYEVVYPEFAA
                     GLAARSELLLTVSNDAWFGTSIGPLQHLQMAQMRALEAGRWMIRATNNGVTALIDPFG
                     RLDAHVPQFQRAVLRGEVVPMQGLTPYLQWRAWPLTGLCALLLGWPLL"
BASE COUNT          509 a         1179 c         1055 g          502 t
ORIGIN      
        1 atcggcttcg gctccaccgc ggtgatcacc ggcgacgtca cccaggtcga cctgccgcgc
       61 ggcaccaagt cgggcctcgc ccacgtggtc gaggtgctgc gcgacgtgcc ggggatcagc
      121 ttcacccact tcaaggccaa ggacgtggtg cgccacccgc tggtgcaacg catcgtcgag
      181 gcctacgacc gctacgagca tcgcctgcaa ggcaagcccg acaagctcaa cgacactgca
      241 agggacggcc gcgatgcaga ttgaactgga tctgcagctg gccaccgagg ccggcgagct
      301 gccggacgaa gcacagctgc gccgctggtg cgaactggcc ctgcgccagc gcaccgcacc
      361 gtcggagctg accatccgca tcgtcgacga agccgaaggc cgcgagttga accacacctg
      421 gcgcggcaag gactacgcca ccaacgtgct gtcgttcccc gccgaggtgc ccgaaggtcc
      481 gggcggcgtt ccgctgctgg acatcccgct gctcggcgac ctggtgatct gcgcccaggt
      541 ggtcgcccgc gaggccggag agcagggcaa gagcctggac gcccactggg cgcacctgac
      601 catccacggc tgcctgcacc tgctcggcta cgatcatatc gacgacgacg aagccgagga
      661 aatggaagcg ctggagcggc agctgctggc cgaactgggc tatcccgacc cctatgcgtg
      721 tgacgaagag taaggacaca tgagcgaaga ccgatcgagc caggggcaga ggtcctggct
      781 ggaaagaatc acccaggcgt tttcccatga gccgaaaagc cgccaggagc tcctcgaagt
      841 cctgcgcgaa gcccatgaga acaagctgct cgacaccgaa gcgctggcga tcgtcgaggg
      901 cgccatccag gtcgccgacc tgcaggtgcg cgacatcatg atcccgcgct cgcagatcat
      961 caccatcaag gcctgccaga gcccccagga gttcctcccg gcggtgatcg acgccgccca
     1021 ctcgcgctac ccggtgatcg gcgagaacct cgacgaggtc gtcggtatcc tgctggccaa
     1081 ggacctgctg ccgctgctgc tgcgcgacag cacacaggcc ttcgacctgc acaagctgat
     1141 gcgtccggcc accttcgtcc ccgaatccaa gcgcctcaac gtgctgctgc gcgagttccg
     1201 cgccaatcgc aaccacatgg ccatcgtcat cgacgaatac ggcggcgtgg ccggcctggt
     1261 gaccatcgag gacgtgctgg agcagatcgt cggcgacatc gaggacgagc acgacgtcga
     1321 ggaggacagc tacatcaagg cgctgcccag cggcgacttc atcgtcaagg cgctgacccc
     1381 ggtggaggac ttcaacgaat ccttcgccac gaccttcgcc gaggacgact tcgacaccat
     1441 cggcggcgtg gtgatgagca ccttcggcca catgcctaaa cgcaacgaag tgactgacat
     1501 cggcggcttc cgcttccgcg tggtgaacgc cgacagccgg cgcatccacc tgttgcgagt
     1561 gacgccgctg gtgcgctgag actggcgagc gaggcgtagg gtggatggcc accccgtgga
     1621 aaactcgcga agcgattttc caccgggacg ccgcgttgtc cgctgggcaa ggcctgcggc
     1681 cgtttcccac cctacccgcc cgcgcgccgc tccgctaagc tgccctgccc cgcattctgc
     1741 aaggattccc catgcgctgg atcacccgcc ccggctggcc aggtcacctg cttgcgctgg
     1801 ctgccggcgc cctcacgccg ctggccctgg cgcccttcga cctgtggccg ctgggcctgc
     1861 tgtcgctggc cctgttctac ctcggcctgc gcggcctggc gccacgggcc gccctggcgc
     1921 gcggctggtg ctacggcttc ggcgccttcg ccgccggcac cagctgggtc tacgtcagca
     1981 tccacgacta cggcgccgcc tcgcccgccc tggccggcct gctgaccctg ctgttcagcg
     2041 ccgggctggc gctgttcttt gccctgccgg cctgggtctg ggtcaaatgg ctgcgccgcg
     2101 agcgcgcgcc gctggccgat gcgctggcct tcgccgccct gtggctggcc caggaagccc
     2161 tgcgcggctg gctgttcacc ggcttcccct ggctgtacat cggctacagc cagctcgccg
     2221 ggccgctggc cgggctggcc ccgctcggcg gcgtctggct gctgtcgttc ggcatggcgc
     2281 tgaccgcggc gctgctggcc aacctgccgg cgctgctgcc acgctggtcg tcggccctga
     2341 tcgccctgct gctgctcgtc gccacctggg gcagcggcct gctgctcagg gaccacgcct
     2401 ggacccagcc ggccggcgca ccgctcaagg tcgccgccat gcagggcaat gtcgaacaga
     2461 gcatgaagtg ggatcccgag cagctcaacg cccagctggc cctgtaccgc gacctgacct
     2521 ttgccgaggc gccggccgac ctgtacgtct ggccggaaac cgcagtgccg gtgctcaagg
     2581 aatacgccga gggctacctc agcgtgatga gccgtttcgc cagcgagcgc aacgccgcgc
     2641 tgatcaccgg cgtaccgttg cgccaggccg acggcgaagg ccagccgcgc tactacaacg
     2701 cggtgcaggt gatcggccag ggcgaaggca cctacctgaa gcagaagctg gtgccgttcg
     2761 gcgagtacgt gccgctgcag gatgtgctgc gcggactgat cgccttcttc gacctgccga
     2821 tgtccgactt cgcccgcggc ccggccgatc agcccatgct gcaggcgcgc ggctaccgca
     2881 tcgcccccta catctgctac gaggtggtct atccggaatt cgccgccggc ctcgccgcgc
     2941 gcagcgagct gctgctgacg gtgagcaacg acgcctggtt cggcacctcg atcggtccgc
     3001 tgcagcacct gcagatggcg cagatgcgtg ccctcgaggc cgggcgctgg atgatccgcg
     3061 ccaccaacaa cggcgtcacc gcgctgatcg acccgttcgg ccgcctcgac gcccatgtgc
     3121 cgcagttcca gcgcgccgtg ctgcgcggcg aggtggtgcc gatgcagggc ctgacgccct
     3181 acctgcagtg gcgcgcctgg ccgctgaccg gcctctgcgc cctgctgctc ggctggccgc
     3241 tgctg
//
LOCUS       DATCUN010000304         5206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_89866, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000304 DATCUN010000000
VERSION     DATCUN010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89866"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VIO83_11685"
     CDS             complement(<1..747)
                     /locus_tag="VIO83_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8331973.1"
                     /translation="MRTELDSCLAAVNQVLLGKDKQVRLALTCLLARGHLLLEDLPGM
                     GKTTLSHTLARVLGLSYQRIQFTSDLLPGDILGTSVFNRETGQFTFHPGPIFAELVLA
                     DEINRATPKSQSALLEAMEEGQVTIEGATRPLPQPFFVIATQNPSSQGGTFALPESQL
                     DRFLMRVSLGYPARSAEKALLLGEARRDLLPRLEPILDHAALARIQAQVPGIHASEAV
                     IDYVLRLVEATRTQPAFALGLSPRGSLALLA"
     gene            1164..1913
                     /locus_tag="VIO83_11690"
     CDS             1164..1913
                     /locus_tag="VIO83_11690"
                     /inference="COORDINATES: protein motif:HMM:NF013293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8331974.1"
                     /translation="MIGFGRTGKSVASVLLESDATALQWVIRGSANPERKSAADCLGV
                     VSDNPALIFGCDEIAIDSLLDRHPVDVIIDFSSADGLELYAREASQRGIAIISAISQY
                     PEERVEQLRALAEHTRVLWSPNITIGINFLILAAKILKQIAPEADIAIIEEHFAAKPE
                     TSGTARVIAETLDQCSDDIKSIRAGGIVGRHEILFGFPYQTVRLSHESIAREAFGNGV
                     LFAAKNLLARDSGFYSMQDLMEPYVYERTDN"
     gene            1993..4683
                     /locus_tag="VIO83_11695"
     CDS             1993..4683
                     /locus_tag="VIO83_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009314801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY8331975.1"
                     /translation="MPPAAPQDWHARSAQQCLEQLHSHAAGLSAEEAERRLRQYGPNL
                     LPETAGPGWFRRLLLQFHNLLIYVLLGAALLVGLLGHRLDALVILGVVAINALVGFIQ
                     EGKAEQAMRSIRKLLTLDSRVRRDGLVCSLPAEQLVPGDIVLLEAGDRVPADLRLLDC
                     RDLRIDEAMLTGESLPADKHCAAVAPAASLGDRHGMAYSGTLVSAGNGTGVVVATGQQ
                     TELGHISGLLGRVESLQTPLLADIQRFARQLTVAILLLAGLTYAVGVFWRDYSPSEML
                     MAAVGLAVAAIPEGLPAVLTIVLALGVQRMARHRAIIRRLPAVESLGAVTVICSDKTG
                     TLTRNEMTVQQVFTTAHAYQIGGVGYEPSGSLSREGCRLAPPPADDLLELARAGLLAN
                     SASLRQGAEGWQISGDPTEAALLTLAGKLGLDGDHELRRLPRVDSIPFSPELRCTASL
                     HHDHAGHGLIYLFGAPERLLELCNRQLRDGNAEPLDPRAWHARLHEGAAGGLRMIGLA
                     MRPLPAPQSQLDYADLEGDFILLGLVGMLDPPREEAIAAIAECRSAGIAVKMITGDHA
                     ATAAVIAERLGLGGGTPLTGAEIDQLDDAALDARLADTTVFARTNPAHKLRLVERLQA
                     RGERVAMTGDGVNDAPALKRADIGIAMGIKGTEAAKEAAQMVLADDNFATLTHAVEEG
                     RTVYDNLRKSILFILPTSGGQSLVLLFAIALGLSNLPITPLQILWVNMITAVTLALAL
                     AFEPAENNLMQRPPRDPAEPLLSGQLLWRLLFVGLLLTGASLGLFLYAEQLGLPLEVA
                     RTLAVNALVAGEIGYLFCARRLQGAASFGLRDNPMVWAMVLLVVPLQLAFSHWAPLQA
                     LFGTAALDARGWALVVACAVAVMLLVEMEKWLLRRRHARR"
     gene            complement(4710..>5206)
                     /locus_tag="VIO83_11700"
     CDS             complement(4710..>5206)
                     /locus_tag="VIO83_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015635258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY8331976.1"
                     /translation="ADAALREFARACCAGQRDRAALIGLMEQLHRRMPFTPGITQVET
                     TAAEAFALGLGVCQDHTHVFLACARSLGIPARYVSGYLCRAGSSELASHAWAEAWVDG
                     AWYSFDVTNRLTRPARHLKLAVGLDYLDACPVRGMRRGGQEQMHALVQEAPLLQNQLP
                     GQQQ"
BASE COUNT          807 a         1854 c         1773 g          772 t
ORIGIN      
        1 cgccagcagc gccaggctgc cgcgcggcga caggccgagg gcgaaggccg gctgggtgcg
       61 ggtggcctcg accaggcgca gcacgtaatc gatcaccgcc tcgctggcat ggatgcccgg
      121 cacctgtgcc tggatacgcg ccagcgcggc atggtcgagg atcggctcga ggcgcggcag
      181 caggtcgcgg cgggcctcgc cgagcagcag ggccttctcg gcgctgcgcg ccgggtagcc
      241 cagcgacacg cgcatcagga agcggtcgag ctgcgactcc ggcaaggcga aggtgccgcc
      301 ctggctggac gggttctgcg tggcgatgac gaagaacggc tgcggcagcg gccgggtggc
      361 gccctcgatg gtcacctggc cctcctccat cgcctcgagc agggcgctct ggctcttcgg
      421 cgtggcgcgg ttgatctcgt cggccagcac cagctcggcg aagatcggcc cgggatggaa
      481 ggtgaactgc ccggtttccc ggttgaacac cgaggtgccg aggatgtcgc cgggcagcag
      541 gtcggaggtg aactggatgc gctgatagct gagccccagc acccgcgcca gggtatggct
      601 gagggtggtc ttgcccatcc ccggcaggtc ttccagcagc agatggccac gtgccagcag
      661 gcaggtcagg gccaggcgca cctgtttgtc cttgccgagc agaacctggt tgacggccgc
      721 caggcagctg tccagttcgg tgcgcatgcg cgtactccct tcgcgaaaag cgctgatcgt
      781 actgggccag cggcgcgagg caaagcggat cggcgccgaa ttgccggcgc atccacgctt
      841 ggcgcaggtt gcggatcccg ccactgggcg agccgccgcc gccaggcggc aaagaccgtg
      901 gttttattgc ggaatattac gacgccgcag cccccgtaac tcgggggctg cggcgcagta
      961 tcccgcaatc gtcacatcca tcgcgcagcc ttggccccgg cctggcgcgt ggacccctcg
     1021 cgccgccatg gggtaaggtc gggacacggt cggtccgccc ggaccagccg caacactttg
     1081 aacgcgcagt cattttcccc gggagtatgc gtattcggac aacaacaatc agacactcga
     1141 gggcatggaa attgaaggtt ggattgatag gtttcggacg cacaggcaag tctgtcgctt
     1201 ccgttctgct tgaaagcgac gccactgcgc tgcagtgggt catccgcggc tcggccaatc
     1261 ccgagcgcaa gtcggccgcc gactgcctgg gcgtggtctc cgacaacccg gcgctgatct
     1321 tcggctgcga cgagatcgcc atcgacagcc tgctcgaccg ccatccggtc gacgtgatca
     1381 tcgacttctc cagcgccgac ggcctcgagt tgtatgcccg cgaggcgagc cagcgcggca
     1441 tcgccatcat cagcgccatc tcccagtatc ccgaggagcg ggtcgagcaa ctgcgggcac
     1501 tggccgagca cacccgggtg ctctggtcgc cgaacatcac catcggcatc aacttcctga
     1561 tcctggcggc gaagatcctc aagcagatcg ctcccgaagc cgatatcgcc atcatcgagg
     1621 agcacttcgc ggccaaaccg gaaacctccg gcaccgccag ggtcatcgcc gaaaccctcg
     1681 accagtgcag cgacgacatc aagtcgatcc gcgccggcgg catcgtcggc cgccacgaga
     1741 ttctcttcgg cttcccctac cagaccgtgc gcctcagcca cgagtccatc gcccgcgagg
     1801 ccttcggcaa tggcgtgctg ttcgccgcca agaacctgct ggcgcgggac agcggcttct
     1861 acagcatgca ggacctgatg gagccgtacg tctacgagcg cacggacaac tgaagcacca
     1921 tactcgactc ttgcggtgcc ggccgagcgg ctgcgccgca gagcatgacc cgcagcaacc
     1981 aggagaacag ccatgccccc ggcagcgcca caggactggc acgcacgcag cgcccaacaa
     2041 tgcctggagc agctgcacag ccacgctgcc gggttgagtg cagaagaggc tgagcgccgc
     2101 ctgcgccagt atggtcccaa cctcctgccg gaaaccgccg ggccgggctg gttccgccgg
     2161 ctgctgctgc agttccacaa cctgctgatc tatgtattgc tgggcgcggc gctgctggtg
     2221 gggctgctcg gccaccggct ggacgcactg gtgatcctcg gcgtggtggc gatcaacgcg
     2281 ctggtcggct tcatccagga gggcaaggcc gaacaggcca tgcgctcgat ccgcaagctg
     2341 ctgaccctcg acagccgggt gcgccgcgat ggcctggtct gctcgctgcc ggccgagcaa
     2401 ctggtgcccg gcgacatcgt cctgctcgaa gccggcgacc gcgtaccggc cgacctgcgc
     2461 ctgctcgact gtcgcgacct gcgcatcgac gaggccatgc tcaccggcga atcactgccg
     2521 gccgacaagc actgcgccgc cgtcgccccc gccgccagcc tcggcgaccg ccacggcatg
     2581 gcctattccg gcaccctggt cagtgccggc aacggcaccg gcgtggtggt ggccaccggc
     2641 caacagaccg aactaggcca catcagcggc ctgctgggcc gggtggaaag cctgcagacg
     2701 ccgctgctgg ccgacatcca gcgtttcgcc cgccagctca ccgtggccat cctgctgctc
     2761 gccggcctga cctatgcggt cggcgtgttc tggcgcgact actcgcccag cgagatgctg
     2821 atggcggccg tcggcctggc ggtggcggcg attcccgaag gcctgccggc ggtgctgacc
     2881 atcgtcctcg ccctcggcgt gcagcgcatg gcccgccatc gcgcgatcat ccgccgcctg
     2941 ccggcggtgg agagcctcgg tgcggtcacg gtgatctgct cggacaagac cggcaccctg
     3001 acccgcaacg agatgaccgt gcagcaggtg ttcaccacgg cgcacgctta ccagatcggc
     3061 ggcgtcggtt atgaacccag cggcagcctg agccgcgaag gctgccggct cgcaccaccg
     3121 ccggccgatg acctgctcga actggcccgc gccggcctgc tggccaacag cgccagcctg
     3181 cgccagggcg ccgaaggctg gcagatcagc ggcgacccca ccgaggcggc gctgctcacc
     3241 ctggccggca agctcggcct ggacggcgac catgagctgc gccgcctgcc gcgggtcgac
     3301 agcattccct tcagcccgga gctgcgctgc accgccagcc tgcatcatga ccacgccggc
     3361 cacggcctga tctatctgtt cggcgctccg gagcggctgc tcgagctgtg caaccgccag
     3421 ttgcgcgacg gcaacgccga gccgctcgat ccacgcgcct ggcatgcccg tctgcacgag
     3481 ggcgcggccg gcggcctgcg catgatcggc ctggccatgc gcccgctgcc ggcaccgcag
     3541 agccagctcg actacgccga cctcgaaggc gacttcattc tcctcggcct ggtcggcatg
     3601 ctcgacccgc cgcgcgagga ggccatcgcc gccatcgccg aatgccgcag cgccggcatc
     3661 gcggtgaaga tgatcaccgg cgaccatgcc gccaccgccg cggtgatcgc cgagcgtctg
     3721 ggcctggggg gcggcacgcc gctgaccgga gccgagatcg accagctcga cgatgccgca
     3781 ctggatgcgc gtctggccga caccacggtg ttcgcccgca ccaacccggc gcacaaactg
     3841 cgcctggtcg agcgtctgca ggcccgcggc gagcgggtgg cgatgaccgg cgacggggtc
     3901 aacgatgcgc cggcactcaa gcgcgccgac atcggcatcg ccatgggcat caagggcacc
     3961 gaggcggcca aggaagccgc gcagatggtg ctggccgacg acaacttcgc caccctcacc
     4021 catgcggtgg aggaaggccg cacggtctac gacaacctgc gcaagtcgat cctgttcatc
     4081 ctgccgacca gcggtggcca gtcgctggta ctgctgttcg ccatcgccct cgggctgagc
     4141 aacctgccga tcacgccgtt gcagatcctc tgggtcaaca tgatcaccgc cgtgaccctg
     4201 gcgctggcgc tggccttcga gccggccgag aacaacctga tgcagcgccc gccgcgcgat
     4261 ccggccgagc cgctgctgtc cgggcaactg ctgtggcgtc tgctgttcgt cggcctgctg
     4321 ctgaccggcg ccagcctcgg cctgttcctt tacgccgagc agctcggcct gccgctggag
     4381 gtcgcccgca ccctggcggt caacgccctg gtggccggcg agatcggcta cctgttctgc
     4441 gcccgccgcc tgcagggggc ggcgagcttc gggctgcgcg acaacccgat ggtctgggcc
     4501 atggtgctgc tggtggtgcc gctgcaactg gcgttcagcc actgggcgcc gctgcaggcg
     4561 ctgttcggca ccgccgcgct ggacgcccgc ggctgggcgc tggtggtggc ctgcgccgtg
     4621 gcggtgatgc tgctggtgga aatggagaaa tggctgctgc gccgacggca cgccaggcgc
     4681 tgacgccggc ccgcggccgg cgccagacct tactgctgct ggccgggcag ctggttctgc
     4741 agcagcgggg cttcctgcac cagggcgtgc atctgctcct ggccgccgcg gcgcatgccg
     4801 cgcaccgggc aggcgtcgag gtagtcgagc ccgaccgcca gcttgaggtg gcgcgcaggg
     4861 cgggtcaggc ggttggtcac gtcgaagctg taccaggcac cgtcgaccca ggcctcggcc
     4921 caggcgtggc tggccagctc gctgctcccg gcacggcaca ggtagccgga cacgtagcgc
     4981 gccggaatgc ccaggctgcg ggcacaggcc agaaacacgt gggtgtggtc ctggcacacg
     5041 ccgaggccga gggcgaaggc ttccgccgcg gtggtctcga cctgggtgat gcccggcgtg
     5101 aagggcatgc gccggtgcag ttgctccatc agcccgatca gcgccgcgcg atcacgctgt
     5161 ccggcgcagc aggcgcgggc gaactcgcgc agcgccgcgt cggccg
//
LOCUS       DATCUN010000305         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_240175, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000305 DATCUN010000000
VERSION     DATCUN010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240175"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2192)
                     /locus_tag="VIO83_11705"
     CDS             complement(<1..2192)
                     /locus_tag="VIO83_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008003123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY8331977.1"
                     /translation="MIERIAEFCIRRRAWVTGVLLTLTLILSWFALRIEVRTVFADML
                     PSRHEYVQTHEKFKDTFGGSNMVTIMFEVEEGDIFQTAVLDKVRSVTLGLREVSAVNQ
                     YQITSLASKKLKEVRASTAGIESRPLMWPDLPVDAEAMAELKRSVLRNPMVYGPYVSK
                     DLQATLVTVDFIDRQVDYSKVFHEVRALIAKVDDGSVKIRVVGDPILYGWVNHYLPET
                     IQLVLAALAVTLVMLFLLLRTWRGLFLPLLAGVVSAIWALGICRLLGIHFEPLVIVVA
                     MLITSRAVSHSVQIVNRFDDEIELLPPGADTSRTAARIALADLFRPGMLGVIADAACM
                     AVVALSPIPMLQKLTVLSVVWVSTLTVSAVILTPVLLSFIRKPHGMAHPLNCLPGLRK
                     VLDLAVLVCLSRARYAVLGVSLVVVVGAGLYALNLKIGDANPGSPILWPQAQYNQDSA
                     AINARFEGVDRMFVVLGDDSRPDLVKGNEALQAMNSFQRFIEAQPEVGGSVSIADVLP
                     QVNASLREGNPRYLELGDTGNLNGSLMAMLDSVSEPGDISRFADEHYANGSVTLMFRD
                     RQGETIRTAVARIKEFIASHPLSEGQWHLAGGLIGVMAAVNEIILSSQIEAIALALLV
                     LAVLCTLVYRSSVAGMLFMVPVIISNMLTFAFMTWKGIGMNINTVPVAALGIGLGVDY
                     AFYIADRVKEEIAIGSSPEHAIRQALHSSGMGVIVTASVLILATLLWWV"
     gene            complement(2228..>2650)
                     /locus_tag="VIO83_11710"
     CDS             complement(2228..>2650)
                     /locus_tag="VIO83_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008047635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEY8331978.1"
                     /translation="EFGRILLSDEQGQNWEEIASPVPASLMSVQFRDALNGVAVGLEG
                     ALLVTRDGGRQWDSVDLGIKDHLFDVLWDAGNGRWFASGALGRWVSGSAAGDWQSGAL
                     DERNLAWHTRALQVGSSIWLAGADVGRWDGRQWSPVNP"
BASE COUNT          446 a          886 c          921 g          397 t
ORIGIN      
        1 acccaccaca gcagggtggc gaggatcagc acgctggcgg tgacgatgac ccccatgccc
       61 gagctgtgca gggcctggcg gatggcgtgt tcggggctgc tgccgatggc gatctcttcc
      121 ttgacgcggt cggcgatgta gaaggcgtag tccaccccca ggccgatgcc gagggcggcc
      181 accggcacgg tgttgatgtt catgccgatg cccttccagg tcatgaaggc gaaggtcagc
      241 atgttggaga tgatcaccgg gaccatgaac agcatgccgg cgacgctgga gcggtagacc
      301 agggtgcaca gcacggcgag caccagcagg gccagggcga tggcctcgat ctggctggac
      361 aggatgattt cgttgaccgc cgccatcacg ccgatcaggc cgccggccag gtgccactgg
      421 ccctcgctca gcggatggct ggcgatgaac tccttgatgc gcgccaccgc ggtgcggatg
      481 gtctcgccct ggcggtcgcg gaacatcagg gtgaccgagc cgttggcgta gtgctcgtcg
      541 gcgaagcgcg agatgtcgcc cggctcggac accgaatcga gcatggccat caggctgccg
      601 ttgaggttgc ccgtgtcgcc cagctccagg tagcgcgggt tgccctcgcg caggctggcg
      661 ttgacctgcg gcagcacgtc ggcgatggac accgagccgc cgacctcggg ctgcgcctcg
      721 atgaagcgct ggaagctgtt catcgcctgc agggcctcgt tgcccttgac caggtcgggg
      781 cggctgtcgt cgccgagcac cacgaacatg cggtcgacgc cttcgaagcg cgcgttgatc
      841 gccgccgagt cctggttgta ctgcgcctgc ggccagagga tcggcgaacc gggattggcg
      901 tcgccgatct tcaggttcag cgcgtacagg ccggcaccga ccaccaccac caggctgacg
      961 ccgagcaccg cgtagcgggc ccgcgacagg cagaccagca cggccaggtc gagcaccttg
     1021 cgcagccccg gcaggcagtt gagcgggtgg gccatgccgt gcggcttgcg gatgaacgac
     1081 agcagcaccg gggtgaggat caccgcgctg acagtcaggg tcgacaccca gaccaccgac
     1141 agcacggtga gcttctgcag catcgggatc ggactcagcg ccaccaccgc catgcatgcg
     1201 gcgtcggcaa tcacccccag catgcccgga cggaacaggt cggccagggc gatccgcgcg
     1261 gcggtccgcg aggtgtcagc gcccggcggc agcagctcga tctcgtcgtc gaagcggttg
     1321 acgatctgca ccgagtgcga caccgcccgc gaggtgatca gcatggccac cacgatcacc
     1381 agcggctcga agtggatgcc cagcaggcgg cagatgccca gcgcccagat cgcgctgacc
     1441 acgccggcca gcagcggcag gaacagcccg cgccaggtgc gcagcaggag gaacagcatg
     1501 accagggtca ccgccagggc ggccagcacc agctggatgg tctccggcag gtagtggttg
     1561 acccagccgt agaggatcgg gtcgccgacc acgcggatct tcacgctgcc gtcgtcgacc
     1621 ttggcgatca gcgcgcggac ctcgtggaat accttgctgt agtcgacctg gcggtcgatg
     1681 aagtcgacgg tgaccagggt ggcctgcagg tccttggaca cgtagggacc gtagaccatc
     1741 gggttgcgca gcaccgagcg cttcagctcg gccatcgcct cggcgtccac cggcaggtcg
     1801 ggccacatca gcgggcgact ctcgatgccc gcggtggagg cgcgcacttc cttgagcttc
     1861 ttcgaggcca gcgaggtgat ctggtactgg ttgaccgccg agacctcgcg caggccgagg
     1921 gtgacgctgc gcaccttgtc gagcacggcg gtctggaaga tgtcgccctc ctcgacctcg
     1981 aacatgatgg tgaccatgtt cgagccgccg aaggtgtcct tgaacttctc gtgggtctgc
     2041 acgtactcgt ggcgcgaggg cagcatgtcg gcgaacacgg tgcgcacctc gatgcgcagc
     2101 gcgaaccagc tgaggatcag ggtcagggtg agcagcacgc cggtcaccca ggcgcgccga
     2161 cggatgcaga attcggctat gcgttcgatc atggtcgtat ctcgcagaag taggggccag
     2221 gggcggttca gggattcacg ggcgaccact ggcggccgtc ccagcggccg acgtcggcgc
     2281 cggccagcca gatgctcgag ccgacctgca gcgcgcgggt gtgccaggcc aggttgcgct
     2341 catccagcgc gccgctctgc cagtcgccgg cggcgctgcc gctcacccag cggcccagcg
     2401 cgccactggc gaaccagcgg ccgttgccgg cgtcccagag cacgtcgaac aggtggtcct
     2461 tgatgcccag gtcgacgctg tcccactgcc ggccaccgtc gcgggtgacc agcagggcgc
     2521 cttccagacc gaccgccacg ccgttcagcg catcgcggaa ctgcaccgac atcagcgagg
     2581 ccggcaccgg gctggcgatc tcctcccagt tctgcccctg ctcgtcgctg agcaggatgc
     2641 ggccgaactc
//
LOCUS       DATCUN010000306         2882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_15450, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000306 DATCUN010000000
VERSION     DATCUN010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15450"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VIO83_11715"
     CDS             <1..1134
                     /locus_tag="VIO83_11715"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY8331979.1"
                     /translation="GNLRAALRSDDTLAHLGGDGFALLLGDLDSAQDCAATLTGLQAV
                     IAAPVTIADSHLHTTASIGVTLYPQDNGDGDTLLRHADQAMFLAKEAGKNCYHLFDPE
                     SDRKAQGHRQQLERLRLALQRGEFVLHYQPKVDLGDGRLFGVEALIRWQDPQRGLIAP
                     GEFLPYIESVGTLERALGEWVLDTALAQLEQWLSSGLQVDMSVNISAHQLMHADFVDS
                     LDRLLCRHPAIPRARLELEILETAAIADMPHAIVVMQRCARLGVRFALDDFGTGYSSL
                     TYLRKLPVDTLKIDQSFVRDMLVDPDDRAIVDGVIRLAAAFNRHVIAEGVETLEHGQA
                     LQRLGCRLAQGYGIARPMPAEHLPVWLRDWQERAAWRQLAAQS"
     gene            1210..1806
                     /gene="wrbA"
                     /locus_tag="VIO83_11720"
     CDS             1210..1806
                     /gene="wrbA"
                     /locus_tag="VIO83_11720"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEY8331980.1"
                     /translation="MKKILVLYYSMYGHIEQMAEAVAAGAASVPGVEVTLKRVPETMP
                     EDLARQAGAKLDQQAALASPQELADYDAILFGTPTRFGNMAGQMRTFLDQTGGLWVKG
                     ALIGKLGSVFTSTGTGGGGETTITSFWHTLAHQGMLIVGLPYSAPELTDTSELRAGSP
                     YGAATIAGSDGSRQPSAKELALARFQGEHVAKLAVRLQ"
     gene            2007..>2882
                     /locus_tag="VIO83_11725"
     CDS             2007..>2882
                     /locus_tag="VIO83_11725"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8331981.1"
                     /translation="MKLLRTTLAALCMSASLAATAAPQSTVTLKVHHFMPNDSLTQRE
                     LIQPWAEKITRESAGRIRFHFFPAMQLGGTPAQLVDQVRDGSADIVWALPGYSSGRFP
                     LTGAFELPFMNRSAEASSQALWDFLQAHGQREYQGIHLLATHLTDSVLLHTRKQPILS
                     MADFAGLKLRTANPVQGRLIGLFGGLPQAMPINPVPAALARGLLDGAAVPWDVATSVK
                     LQKRVKHHTEMAEGMPKLMHAALVLAMNQARYDSLPADLRQIIDANSGRALSAQAGRL
                     WDTRVEENGRQLARAQ"
BASE COUNT          465 a         1096 c          937 g          384 t
ORIGIN      
        1 ggcaacctgc gcgcggcgct gcgcagcgac gacaccctgg cccacctggg cggcgacggc
       61 ttcgccctgc tgctcggcga cctcgactcc gcccaggact gcgcggcgac cctcaccggc
      121 ctgcaggcgg tcatcgccgc gccagtgacc atcgccgaca gccacctgca caccacggcg
      181 agcatcggtg tgaccctcta cccgcaggac aacggcgacg gcgacaccct gctgcgccat
      241 gccgaccagg cgatgttcct ggccaaggag gccggcaaga actgctacca cctgttcgac
      301 ccggaaagcg accgcaaggc ccagggccac cgccagcagc tggagcgcct gcgcctcgcc
      361 ctgcagcggg gcgagttcgt gctgcactac cagcccaagg tcgacctcgg cgacggccgg
      421 ctgttcggcg tcgaggcgct gatccgctgg caggacccgc agcgcgggct gatcgcgccg
      481 ggcgaattcc tcccctacat cgagagcgtc gggaccctgg agcgggcgct gggcgaatgg
      541 gtactggaca cggcactggc ccagctcgag cagtggctga gcagcgggct gcaggtcgac
      601 atgagcgtca acatcagcgc ccaccagctg atgcacgccg acttcgtcga cagcctggat
      661 cgcctgctgt gccgccaccc cgcgattccc cgcgcccggc tggaactgga gatcctcgaa
      721 accgcggcga tcgccgacat gccgcacgcc atcgtggtca tgcagcgctg cgccaggctg
      781 ggcgtgcgct tcgccctcga cgacttcggc accggctact cctcgctcac ctacctgcgc
      841 aagctgccgg tggacaccct gaagatcgac cagagcttcg tgcgcgacat gctggtcgac
      901 cccgacgacc gcgccatcgt cgacggcgtg attcgcctgg ccgccgcctt caaccgccac
      961 gtgatcgccg agggcgtgga aaccctcgag cacggccagg ccctgcagcg tctcggctgc
     1021 cgtcttgccc agggctacgg catcgcccgg ccgatgccgg cggagcacct gcccgtctgg
     1081 ctgcgcgact ggcaggagcg ggccgcctgg cggcagctgg ccgcgcagtc ctgaacgaac
     1141 gacggacgag gcggcggccg cccgccaccc tgctaggctg aacgggacac cctgtctgga
     1201 ggctcgtcga tgaagaagat cctggtgctg tactactcga tgtacgggca tatcgagcag
     1261 atggccgagg cggtggccgc cggcgcagcc agcgtgccgg gggtcgaagt gaccctcaag
     1321 cgcgtgccgg aaaccatgcc cgaggacctg gcgcgccagg ccggcgccaa gctcgaccag
     1381 caggcggcgc tggcctcgcc gcaggagctg gccgactacg acgccatcct gttcggcacg
     1441 ccgacccgct tcggcaacat ggccgggcag atgcgcacct tccttgacca gaccggcggc
     1501 ctgtgggtca agggcgcgct gatcggcaag ctcggcagcg tattcacctc caccggcacc
     1561 ggcggcggcg gggaaaccac catcacctcg ttctggcaca ccctcgccca ccagggcatg
     1621 ctcatcgtcg gcctgcccta cagcgcgccg gagctgaccg acaccagcga gctgcgcgcc
     1681 ggctcgccct acggcgcggc gaccatcgcc ggcagcgacg gctcgcgcca gcccagcgcc
     1741 aaggaactgg ccctggcgcg tttccagggc gagcacgtgg cgaagctggc ggtgcgcctg
     1801 cagtagtcgc ccggcgcggc cgcttccgtg caagcgcagg acggccgctg cctgccacgg
     1861 ccgtttccca ccgtttgtgt gcgcctaccg cccacaaacc accgcaggag attgtcgccg
     1921 gtctcggccg cgcaccagca taggcggcct gcccggccgg gcagcattgg cactgccatc
     1981 acccaacaag aaaaaggaca gtcacggtga aactcctgcg caccactctc gccgcactct
     2041 gcatgagtgc cagcctggcg gccacggccg ccccccagtc gaccgtcacc ctcaaggtgc
     2101 accacttcat gccgaatgac tcgctgaccc agcgcgagct catccagccc tgggcggaga
     2161 agatcacccg cgaatccgct ggccgcatcc gtttccactt cttcccggcg atgcagctcg
     2221 gcggcacgcc ggcgcagctg gtcgaccagg tgcgcgacgg ctcggcggat atcgtctggg
     2281 ccctgcccgg ctacagcagc gggcgcttcc cgctgaccgg ggccttcgaa ctgccgttca
     2341 tgaaccgctc tgccgaagcc agcagccagg ccctgtggga cttcctgcag gcccacggcc
     2401 agcgcgagta ccagggcatc catctgctcg ccacccacct gaccgacagc gtgctgctgc
     2461 acacgcgcaa gcagccgatc ctcagcatgg ccgacttcgc cggcctcaag ctgcgcaccg
     2521 ccaacccggt gcagggccgg ctgatcggcc tgttcggcgg cctgccgcag gccatgccga
     2581 tcaacccggt gcccgccgcg ctggcccgcg gcctgctcga cggcgctgcc gtgccgtggg
     2641 acgtggccac ctcggtcaag ctgcagaagc gggtcaagca ccacaccgag atggccgagg
     2701 gcatgcccaa gctgatgcac gcggccctgg tgctggcaat gaatcaggcc cgctacgaca
     2761 gcctgccggc cgacctgcgg cagatcatcg acgccaacag cggccgcgcc ctgtcggccc
     2821 aggctggccg cctgtgggac acccgcgtgg aggaaaacgg ccggcagctg gcccgcgccc
     2881 ag
//
LOCUS       DATCUN010000307         7345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_240261, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000307 DATCUN010000000
VERSION     DATCUN010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240261"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..949)
                     /locus_tag="VIO83_11730"
     CDS             complement(62..949)
                     /locus_tag="VIO83_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY8331982.1"
                     /translation="MASHEQYYVPEQSKWPIIASIGLLVTVVGLGTWFNDLKAARADS
                     TGPLVFFVGALLLAWMLFGWFGNVIQESRSGLYSGQMDRSFRWGMRWFIFSEVMFFAA
                     FFGALFYLRVFVGPWLDGAGDKGVSAMLWPDFQFAWPLLDNPDPKAYPAPQGIIDPWH
                     LPLINTVLLVTSSFTLTFAHHALRHDKRGALKAWLALTVLLGAAFLVLQAEEYVHAYT
                     ELDLTLRSGVYGATFFMLTGFHGAHVTIGALMLLVMLIRILRGHFAPAQHFGFEAASW
                     YWHFVDVVWLGLFVFVYVL"
     gene            complement(971..1513)
                     /locus_tag="VIO83_11735"
     CDS             complement(971..1513)
                     /locus_tag="VIO83_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY8331983.1"
                     /translation="MNDALPIHRLVTRLMLVVVAMFGFGFALVPLYDVMCQAFGINGK
                     TAGAYAGVQAVDEARTVRVQFLATNAAGMTWAFAPQQDELSVHPGASNQMLFVAHNPT
                     DHVMTAQAVPSVAPSKAAAFFHKTECFCFTQQVLQPGERIEMPVRFIVDRDLPKDVQH
                     LTLGYTLFDITEQKPPLAQY"
     gene            complement(1587..3188)
                     /gene="ctaD"
                     /locus_tag="VIO83_11740"
     CDS             complement(1587..3188)
                     /gene="ctaD"
                     /locus_tag="VIO83_11740"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY8331984.1"
                     /translation="MSAVIDSHPHGHVHGPAKGLMRWVLTTNHKDIGTLYLVFSFAMF
                     LFGGSMAMVIRAELFQPGLQIVQPEFFNQMTTMHGLVMVFGAVMPAFVGLANWMIPLM
                     IGAPDMALPRMNNFSFWLLPAAFGLMASTLFMAGGGPNFGWTFYAPLSTTYAPESVTF
                     FIFAVHLMGISSIMGAINVIATVLNLRAPGMTLMQMPLFVWTWLITAFLLIAVMPVLA
                     GVVTMMLMDIHFGTSFFSAAGGGDPVLFQHVFWFFGHPEVYIMILPAFGAVSAIIPTF
                     SRKPLFGYTSMVYATGAIAFLSFIVWAHHMFTVGIPLTGELFFMYATMLIAVPTGVKV
                     FNWASTMWRGSLSFEAPMLFAVAFVILFTIGGFSGLMLAIAPADFQYHDTYFVVAHFH
                     YVLVPGAIFGIFASAYYWLPKWTGHLYDETLAKLHFWLSFVGMNLAFFPMHFVGLAGM
                     PRRIPDYNLMFANFNMVSSIGAFLFGATQLLFLFIVIKCVRGGPVAAAKPWDGAHGLE
                     WTLPSPAPYHTFQTPPQVDERSRLH"
     gene            complement(3194..4318)
                     /gene="coxB"
                     /locus_tag="VIO83_11745"
     CDS             complement(3194..4318)
                     /gene="coxB"
                     /locus_tag="VIO83_11745"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY8331985.1"
                     /translation="MSRHPRLWLGAILCLAILPAQASWTVNMSPGATEVSRSVFDLHM
                     TIFWICVIIGVVVFGAMFWSMLMHRRSQGQQPAQFHEHTWVEILWTVVPFAILVLMAV
                     PATRTLLDIYDTAESDLDIQVTGYQWKWHYKYLGQDVEFFSNLATPRAQIDNQAPKDE
                     QYLLEVDNPLVVPVGAKVRFLVTATDVIHSWWVPALAVKKDAIPGFVNEAWTRIEQPG
                     IYRGQCTELCGKDHGFMPVVVEAKAPADFALWLAERKAEALKAQELTGKQWTFDELMA
                     RGEKAYLTTCAACHQPGGEGLPPMFKALKGSPIATGPKEGHLAIVLNGKPGTAMAAFG
                     KQLSEVDLAAIITYERNAWGNNSGDMVTPQDIVAFKQAQE"
     gene            4633..5265
                     /locus_tag="VIO83_11750"
     CDS             4633..5265
                     /locus_tag="VIO83_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017132155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331986.1"
                     /translation="MNIAALRERIDSALQHEVAHPPLVADLAARIDNLHRSIRLPAED
                     APGALLRFVHAYIEKVPAQVEAAAEVASAAGIDAQIKPVLRQAATFLLSPPPFMQEYQ
                     GLAAMLGEAYLAHRLVEEVNDRYIAHFGQPLIPLDTTVANLVAHQLIGEPLANQLDAA
                     VHQASDTLQDERIFATESADHYRAQLAEAAQDHAWPCLSRQLGIELQLAH"
     gene            complement(5280..5999)
                     /locus_tag="VIO83_11755"
     CDS             complement(5280..5999)
                     /locus_tag="VIO83_11755"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY8331987.1"
                     /translation="MNEPVQKKETLENADEALSNIVEGFKRFRRDVFPAQQELFKKLA
                     TAQNPRAMFITCADSRIVPELITQSSPGDLFVTRNVGNVVPPYGQMNGGVSTAIEYAV
                     VALGVRHIIICGHSDCGAMKAVLAPQSLESMPTVKGWLRHAEVARTVVEQNFARCDCG
                     QEHLELLTEENVVAQLNHLLTHPSVAARVASGQLFIHGWVYDIESCNIIAYDAESGRF
                     LPLDGAVVPMATPRSRFNLPG"
     gene            complement(6147..>7345)
                     /locus_tag="VIO83_11760"
     CDS             complement(6147..>7345)
                     /locus_tag="VIO83_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8331988.1"
                     /translation="MRLRDGLQLTAGALLGRPLRSLLTLLGVAIGIAAVALLTAIGAG
                     LRFYMLDTFSQFGTRIVAIHPGRTQTGGIGGLLASARPLTVADAEALRGLGHIEAVVP
                     IIQGSGAIRFGPRSRSSDILGAGHQLAEAWRFRLALGQFLPPARDGRSPPQAVLGHKL
                     RVELFGAANPLGELVRIGGTRFRVVGVMAEKGQMLGFDLDDVAYVPVDWAESLFNREG
                     LMEINVMYRPSTSSKALAERIRGLLIERHGAEDFSLTTQDEMLASLDRILAILSAAVA
                     GLGGISLLVGAVGILTIMTTTVSERTAEIGLLCALGARPRQVLGLFLAEAVLLSLAGG
                     LLGLLLLAVLLGVLHLAVPDLPLRPQPLFLLLALLLSALVGILAGLAPARKAAQLHPV
                     DALRSE"
BASE COUNT         1307 a         2383 c         2532 g         1123 t
ORIGIN      
        1 gcgaaagcgc gccgcgcgcc gccgcgcggc cagactcgcc atgccggcca tcgcgccccc
       61 gtcacagcac gtagacgaag acgaacaggc cgagccagac cacgtcgacg aagtgccagt
      121 accagctggc cgcctcgaag ccgaagtgct gcgccggcgc gaagtgcccg cgcaggatgc
      181 ggatcagcat caccagcagc atcagcgccc cgatggtgac gtgggcgccg tggaagccgg
      241 tgagcatgaa gaaggtcgcg ccgtacacgc cggagcgcag ggtcaggtcc agctcggtgt
      301 aggcatgcac gtactcctcg gcctgcaaca ccaggaaggc cgcgccgagc agcacggtca
      361 gcgccagcca ggccttgagt gcgccgcgct tgtcgtggcg cagggcgtgg tgggcgaagg
      421 tcagggtgaa gctggaggtc accagcagca cggtattgat cagcggcagg tgccaggggt
      481 cgatgatccc ctgcggcgcc gggtaggcct tgggatcggg gttgtccagc agcggccagg
      541 cgaactggaa gtccggccac agcatggcgc tgacgccctt gtcgccggcg ccgtccagcc
      601 aggggccgac gaacacccgc aggtagaaca gcgcgccgaa gaaggcggcg aagaacatca
      661 cctcggagaa gatgaaccag cgcatgcccc agcggaacga gcggtccatc tgcccgctgt
      721 acaggccgct gcggctctcc tggatgacgt tgccgaacca gccgaacagc atccaggcca
      781 gcagcagcgc gccgacgaag aacaccagcg ggccggtgga gtcggcgcgc gccgccttga
      841 gatcgttgaa ccaggtgccg aggccgacca cggtcaccag caagccgatg ctggcgatga
      901 tcggccactt gctctgttcc ggaacgtagt actgctcgtg gctggccatt gttgttctcc
      961 tgtcgtcgcg ctagtactgg gccagcggcg gcttttgctc ggtgatgtcg aacagcgtgt
     1021 aacccagggt caggtgctgg acgtccttcg gcaggtcgcg gtcgacgatg aagcgcaccg
     1081 gcatctcgat gcgctcgccc ggctgcagca cctgctgggt gaaacagaag cactcggtct
     1141 tgtggaaaaa agccgcggcc ttcgacggcg ccacgctggg caccgcctgg gcggtcatca
     1201 catggtcggt cgggttatgg gcgacgaaca gcatctggtt gctggcgccg ggatgcacgc
     1261 tgagctcgtc ctgctgcggg gcgaacgccc aggtcatgcc ggcggcattg gtggcaagga
     1321 actgcacgcg caccgtgcgc gcctcgtcca ccgcctgcac ccccgcatag gccccggcgg
     1381 tcttgccgtt gatgccgaag gcctggcaca tcacgtcgta cagcggcacc agggcgaagc
     1441 cgaatccgaa catggccacc accaccagca tcaggcgggt gaccaggcgg tggatcggca
     1501 atgcgtcgtt catggcgctc tcccgcaggg cggaccgcag cccacccgct tgctcatcgg
     1561 tgggccgcag acccacgccg cgaagttcag tgcagccggc tgcgctcgtc cacttgcggc
     1621 ggggtctgga aggtgtggta gggcgccggc gaaggcagcg tccactccag gccatgggcg
     1681 ccgtcccagg gcttggccgc ggccaccggg ccgccgcgca cgcacttgat gacgatgaac
     1741 aggaacagca gctgggtggc gccgaacagg aaggcgccga tgctggagac catgttgaag
     1801 ttggcgaaca tcaggttgta gtcggggatg cgccgcggca tgccggcgag gccgacgaag
     1861 tgcatgggga agaaggccag gttcatgccg acgaagctca gccagaagtg cagcttggcc
     1921 agcgtctcgt cgtacaggtg cccggtccac ttcggcagcc agtagtaggc cgaggcgaag
     1981 atgccgaaga tcgcccccgg caccagcacg tagtggaagt gcgccaccac gaagtaggtg
     2041 tcgtggtact ggaagtcggc cggggcgatg gccagcatca gcccggagaa gccgccgatg
     2101 gtgaacagga tgacgaaggc cacggcgaac agcatcggcg cctcgaagct cagcgaaccg
     2161 cgccacatgg tgctcgccca gttgaacacc ttcaccccgg tgggcacggc gatcagcatg
     2221 gtggcgtaca tgaagaacag ctcgccggtc agcgggatgc cgacggtgaa catgtggtgg
     2281 gcccagacga tgaacgacag gaaggcgatg gcgccggtgg cgtagaccat cgaggtgtag
     2341 ccgaacagcg gcttgcggct gaaggtgggg atgatcgcgc tgaccgcgcc gaaggcgggc
     2401 aggatcatga tgtacacctc gggatggccg aagaaccaga acacgtgctg gaacagcacc
     2461 gggtcgccgc cgccggcggc gctgaagaag ctggtgccga agtggatgtc catcagcatc
     2521 atggtcacca ccccggccag caccggcatc accgcgatca gcaggaaggc ggtgatcagc
     2581 caggtccaca cgaacagcgg catctgcatc agggtcatcc ccggcgcgcg caggttgagc
     2641 acggtggcga tcacgttgat cgcgcccatg atcgagctga tgcccatcag gtgcacggcg
     2701 aagatgaaga aggtgacgct ctccggcgcg taggtggtgg acagcggcgc gtagaaggtc
     2761 cagccgaagt tggggccacc gcccgccatg aacagggtgc tggccatcag gccgaaggcc
     2821 gccggcagca gccagaagct gaagttgttc atccgcggca gggccatgtc cggcgcaccg
     2881 atcatcagcg ggatcatcca gttggccagg ccgacgaagg ccggcatcac cgcgccgaac
     2941 accatgacca ggccgtgcat ggtggtcatc tggttgaaga attccggctg gacgatctgc
     3001 aggccgggct ggaacagctc ggcgcggatg accatggcca tgctgccgcc gaacaggaac
     3061 atggcgaagc tgaacaccag gtacagcgtg ccgatgtcct tgtggttggt ggtcagcacc
     3121 cagcgcatca gccctttggc cgggccgtgg acatggccat gcgggtgcga gtcgatcact
     3181 gcgctcatgt ccgtcactcc tgtgcctgct tgaacgccac tatgtcctgc ggcgtgacca
     3241 tgtcgccgct gttgttgccc caggcgttgc gttcgtaggt gatgatcgcg gccaggtcga
     3301 cctcggacag ctgcttgccg aaggcggcca tggcggtgcc cggcttgccg ttgaggacga
     3361 tggccagatg gccttccttc ggcccggtgg cgatcggcga gcccttgagc gccttgaaca
     3421 tcggcggcag gccctcgccc cccggctggt ggcaggcggc gcaggtggtc aggtaggcct
     3481 tctcgccgcg cgccatcagc tcgtcgaagg tccattgctt gccggtcagc tcctgggcct
     3541 tgagcgcctc ggccttgcgc tcggccagcc agagggcgaa gtccgcgggg gccttggcct
     3601 ccaccaccac cggcatgaag ccgtggtcct tgccgcacag ctcggtgcac tggccgcgat
     3661 agatgccggg ctgctcgatg cgcgtccacg cctcgttgac gaagccgggg atggcgtcct
     3721 tcttcaccgc cagcgcgggc acccaccagg aatggatcac gtcggtagcg gtgaccagga
     3781 agcgcacctt ggcgccgacc ggcaccacca gcgggttgtc cacctcgagc aggtactgct
     3841 cgtccttggg ggcctggttg tcgatctgcg cgcgcggggt ggcgaggttg ctgaagaact
     3901 cgacgtcctg accgaggtac ttgtagtgcc acttccactg gtaaccggtg acctggatgt
     3961 ccaggtccga ttcggcggtg tcatagatgt cgagcagggt gcgggtcgcc ggtacagcca
     4021 tcagcacgag gatggcgaag ggcaccacgg tccagaggat ttccacccag gtatgttcgt
     4081 ggaactgcgc cggctgctgc ccctggctgc ggcggtgcat gagcatcgac cagaacatgg
     4141 cgccgaacac caccacgccg atgatcacgc agatccagaa gatggtcatg tgcaggtcga
     4201 acaccgaacg gctgacctcg gtggcgcctg ggctcatgtt cactgtccag gacgcctgtg
     4261 ccggcagtat cgccaggcaa agaatggctc ccagccataa acgcggatgt cgcgacattc
     4321 cgggtcccct tctttcttgt tttattccgc cggcttcttg cctgcgccaa gggaggaaac
     4381 ggcgtcgaaa ctgctgacca ccaccgccga acgctacccc gcccgaccgg ctgccggttc
     4441 cttcagtgca cacctgacaa ttcgagtata gacaggcatc gaagactggc cagcggaccc
     4501 actgacggca gcgcccaagg cagggggtga tcacccgaat gagaagtctt tgccttgctg
     4561 cagatcaacc gggggcatgc ctgctgtgcc accatggcag catttccccc tgctcgacgg
     4621 agcacctcat ccatgaacat cgccgccctg cgtgagcgca tcgactcggc cctgcaacac
     4681 gaagtcgctc atccccctct ggtcgccgac ctcgcagcgc ggatcgacaa cctgcaccgc
     4741 tccatccgcc tgcccgcgga ggatgcgccg ggggctctgc tgcgcttcgt gcatgcttat
     4801 atcgagaagg tgccggcgca ggtggaagcc gctgccgaag tggcgagcgc ggccggcatc
     4861 gacgcgcaga tcaagccggt gctgcgccag gccgcgacgt tcttgctcag cccgccgccg
     4921 ttcatgcagg agtaccaggg cctggccgcc atgctgggcg aagcctatct ggcccatcgc
     4981 ctggtggagg aggtcaatga ccgctacatc gcccatttcg gccagccgct gatcccgctg
     5041 gataccacgg tggccaatct ggtggcgcac cagctgatcg gcgagccatt ggccaaccag
     5101 ctcgatgcgg ccgtgcatca ggccagcgac acgctgcagg atgagcgcat cttcgccacc
     5161 gagtcagcgg atcactatcg cgcgcaactg gccgaggcgg cgcaggacca cgcctggcca
     5221 tgcctgtcgc gccagctggg catcgagctg cagctggccc actgacgccg cggccggact
     5281 cagcctggca ggttgaagcg cgaacgcggg gtggccatcg gcaccaccgc gccgtcgagc
     5341 ggcaggaagc ggccgctttc cgcatcgtag gcgatgatgt tgcagctctc gatgtcgtag
     5401 acccagccgt ggatgaacag ctggccgctg gccacccgcg ccgccaccga gggatgggtc
     5461 agcaggtggt tgagctgggc caccacgttt tcctcggtga gcagctccag atgctcctgg
     5521 ccgcagtcgc agcgggcaaa gttctgctcc accacggtgc gcgccacctc ggcgtgccgc
     5581 agccagccct ttaccgtggg catgctttcc agcgactgcg gggcgagtac cgccttcatc
     5641 gcgccacaat cggagtggcc gcaaatgatg atgtggcgta cacccaaggc gaccaccgcg
     5701 tattcgatgg cggtggatac gccgccgttc atctggccgt agggcggcac cacgttaccg
     5761 acgttgcggg tgacgaacag gtcgcccggc gagctctggg tgatcagctc gggaacgatg
     5821 cgcgagtcgg cgcaggtgat gaacatggcg cgcgggttct gcgcggtggc gagcttcttg
     5881 aacagttcct gctgagcggg gaacacgtcg cggcgaaagc gcttgaagcc ctcgacgatg
     5941 ttgctcaggg cctcgtcggc gttctccagg gtctctttct tctgcacggg ctcattcacg
     6001 atcagttctc cgaacctttt tttggcgcgt ccaccaggct tgtggcggcg gacgcttttg
     6061 ggtgttctaa ccgacaacgg cggcggcggt aaagtcacac tcttgcactt gggctctgta
     6121 acggaatctg tcggcgccga gcctgctcac tcgctgcgca gcgcatccac cgggtgcagc
     6181 tgggcggcct tccgagccgg ggcgagaccg gcgaggatac cgaccagcgc cgaaagcagc
     6241 agggccagca ggaggaacag cggctgcgga cgcagcggca gatccggcac ggccaggtgc
     6301 aggacgccga gcagcacagc cagcagcagc aggccgagca gaccgccggc cagcgacagc
     6361 agcacggcct cggcgaggaa caggccgagc acctgccgcg ggcgggcgcc gagggcgcac
     6421 agcaggccga tctcggcggt gcgctcgctc accgtggtgg tcatgatggt gaggatgccc
     6481 acggcgccga ccagcaggga gatgccgccg agcccggcca ccgccgcgct gaggatggca
     6541 aggatgcggt cgaggctggc gagcatctcg tcctgggtgg tcaggctgaa gtcctcggcg
     6601 ccgtggcgct cgatcagcag gccgcggatc cgctcggcca gcgccttgga gctggtggac
     6661 gggcggtaca tcacgttgat ctccatcagc ccctcgcggt tgaacaggct ctcggcccag
     6721 tccaccggga cgtaggccac gtcgtcgaga tcgaagccga gcatctggcc cttttccgcc
     6781 atcaccccga ccacccggaa acgcgtgccg ccgatgcgca ccagctcgcc gagcggattg
     6841 gcggcgccga acagttcgac gcgcagcttg tggccgagca ccgcctgcgg cggcgagcgg
     6901 ccgtcacgcg cgggcggcag gaactggccg agagccagac ggaagcgcca ggcctcggcc
     6961 agctgatgac cggcgccgag aatgtcgctg ctgcggctgc gcgggccgaa gcggatcgcg
     7021 ccgctgccct ggatgatcgg caccaccgcc tcgatgtggc ccaggccgcg cagggcctcg
     7081 gcgtcggcca cggtcaatgg ccgggcgctg gccagcaggc cgccgatgcc gccggtctgg
     7141 gtgcgcccgg gatggatggc gacgatgcgc gtgccgaact gcgagaaggt atccagcatg
     7201 tagaagcgca ggcctgcgcc gatggcggtg agcagcgcca ccgcggcgat gccaatggcc
     7261 acgccgagca gggtcagcag gctgcgtagc ggccggccga gcagtgcgcc ggcggtcagc
     7321 tgcaggccat cgcgcaggcg catca
//
LOCUS       DATCUN010000308         2704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_278886, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000308 DATCUN010000000
VERSION     DATCUN010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278886"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VIO83_11765"
     CDS             complement(<1..365)
                     /locus_tag="VIO83_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015502401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY8331989.1"
                     /translation="MPWKRVISVIFTLLVFTAAVLLVRTLWLHYMDAAWTRDGRVRAE
                     VVNIAPDVAGLVVEVAVHDNQTVHKGDLLLRIDAEHYRTAVRQAEALVAARKAELNMR
                     EDNAARRADMNERVVSRENR"
     gene            complement(388..588)
                     /locus_tag="VIO83_11770"
     CDS             complement(388..588)
                     /locus_tag="VIO83_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HEY8331990.1"
                     /translation="MPREIALFGVYLPNLTLLFVITLAVGWGIDRLVAWSGLYRHAWH
                     PTLLRVSLFTCLYGALMLFVYH"
     gene            complement(578..>2704)
                     /locus_tag="VIO83_11775"
     CDS             complement(578..>2704)
                     /locus_tag="VIO83_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY8331991.1"
                     /translation="RRGLDEWAQSEGMIWAFIFKALLTAFIALWLAYRLELPQANTVL
                     ATVFIVMQPESGQVLAKSFYRILGTLLGLTVTVPLIALFAQERVLFLLCLSLWIGVCT
                     AGAARYRDFRGYGCVLAGYTAALIGLPSIAAPEGAFMQALWRVLEISLGILCSGLVSA
                     LILPQNSSTSLRRLLATRFRDFAGFAGAALAGQLDGQRLEQLNARFAGEAVVLDNLRN
                     ASAFEDPHFRMRSGRLGRLNHEFMVLSTRFHGLHQLLQRLQGEAGEQVRTALAPCLAG
                     VGAVLAPLGQHLCSEADAEVLAQRLSDSKRELMQQIRDARQQLAAQQPSDGQLLDFNT
                     AAEQLYRFTSDLHDYALTHASLLVHRHAREQWQDSFTPRANSLAAAVTGMRSALLVLL
                     LGGFWIATAWPSGETCVLTAAMVAGLMASAPSPWQQSLRLLIGAMGSAVLGGLLQFWV
                     LPWLDGFALLFCALAPVFAFGAWLLARSQTAGYGTGLLLWFCIVALPANLPAYDFHRL
                     LNEYLAALLAIGLTTLSLALILPPDRPWLWRRLEHDLRMRVVFAVSGRLKGLVAGFES
                     GTRDLLNQAQVSAVGRPEVQRRLLRWMFAVLEIGQAVIELRQEQARLPAEPCYGEGMP
                     WQVAIRALGRALVRLFIQPDEGNRLRALAAVEQAIATVRQTPENRPPQFDSSPLRRVL
                     SYLHFIRSSLLDPNSPLAAGSFVDAA"
BASE COUNT          447 a          928 c          982 g          347 t
ORIGIN      
        1 cggttctcgc gcgacaccac ccgctcgttc atgtcggcgc gccgcgcggc gttgtcctcg
       61 cgcatgttca gctcggcctt gcgggccgcg accagcgcct cggcctggcg cacggcggtg
      121 cggtagtgct cggcatcgat gcgcagcagc aggtcgccct tgtgcaccgt ctggttgtcg
      181 tgcaccgcca cctcgaccac caggccggcg acgtcggggg cgatgttgac cacctcggcg
      241 cgcacccggc cgtcgcgggt ccaggccgcg tccatgtagt gcagccagag cgtgcgcacc
      301 agcagcacgg cggcggtaaa gaccagcagg gtgaagatga cgctgatgac tcttttccag
      361 ggcatatcca ggctctatcg cggcagatca gtggtagacg aacagcatca gggcgccgta
      421 caggcaggtg aacaggctca cccgcagcag cgtcgggtgc caggcatggc ggtacaggcc
      481 gctccaggcc accaggcggt cgatgcccca gccgacggcc agggtgatga cgaacagcag
      541 ggtcaggttc ggcaggtaga cgccgaacag ggcgatttca cgcggcatca acgaaactcc
      601 cggcggccag cggcgaattg ggatcgagca gcgagctgcg gatgaagtgc aggtagctga
      661 gcacccggcg cagcggcgaa ctgtcgaact gcggcgggcg gttctccggg gtctggcgca
      721 cggtggcgat ggcctgctcc accgcggcca gggcgcgcag gcggttgccc tcgtcgggct
      781 ggatgaacag gcgcaccagc gcccggccga gggcgcggat ggcgacctgc cagggcatgc
      841 cctcgccgta gcagggctcg gccggcaggc gcgcctgctc ctggcgcagc tcgatcaccg
      901 cctggccgat ctccagcacc gcgaacatcc agcgcagcag gcggcgctgc acctcggggc
      961 ggccgaccgc actgacctgg gcctggttga gcaggtcgcg ggtgccgctc tcgaaaccgg
     1021 cgaccaggcc cttgagccgg ccgctgacgg cgaacaccac gcgcatgcgc aggtcgtgct
     1081 ccaggcgccg ccacagccag gggcggtcgg gcggcaggat cagcgccagg ctcagggtgg
     1141 tcaggccgat cgccagcagg gcggccaggt actcgttgag caggcgatgg aaatcgtagg
     1201 ccggcaggtt ggccggcagg gcgacgatgc agaaccacag cagcaggccg gtgccgtagc
     1261 cggcggtctg gctgcgcgcc agcagccagg cgccgaaggc gaacaccggc gccagcgcgc
     1321 agaacagcag ggcgaagccg tccagccacg gcagcaccca gaactgcagc aggccgccca
     1381 gcacggcgct gcccattgca ccgatcagca gcctgagcga ctgctgccac gggctgggcg
     1441 cactggccat caggccggcg accatcgccg cagtcagcac gcaggtttcg ccgctgggcc
     1501 aggccgtggc gatccagaag ccgccgagca gcagcaccag cagggcgctg cgcatcccgg
     1561 tcaccgccgc ggccaggctg ttggcgcgcg gggtgaagct gtcctgccac tgctcgcggg
     1621 catggcggtg gaccagcagc gaggcgtggg tcagcgcgta gtcgtgcagg tcgctggtga
     1681 agcgatagag ctgctcagcc gcggtattga agtcgagcag ttgcccgtcg ctcggctgct
     1741 gcgcagccag ctgctggcgg gcatcgcgga tctgctgcat cagctcgcgc ttgctgtcgc
     1801 tcaggcgctg cgccagcacc tcggcgtcgg cctcgctgca caggtgctgg ccgagcggcg
     1861 ccagtaccgc accgaccccg gccaggcacg gcgccagcgc cgtgcgcacc tgctcgccgg
     1921 cctcgccctg caggcgctgc agcagctggt gcaggccgtg gaagcgggtc gagagcacca
     1981 tgaactcgtg gttcaggcgg cccagccggc cgctgcgcat gcggaaatgc gggtcctcga
     2041 aggcgctggc gttgcgcagg ttgtcgagca ccaccgcctc gccggcgaag cgcgcattga
     2101 gctgctccag gcgctggccg tcgagctgac cggccagcgc cgcgccggcg aaaccggcga
     2161 agtcgcgaaa gcgcgtggcc agcaggcggc gcaggctggt actgctgttc tgcggcagga
     2221 tcagcgcact gaccagcccc gagcagagga tgcccaggct gatctccagc acccgccata
     2281 gcgcctgcat gaaggcgcct tccggggcag cgatggacgg caggccgatc agcgcggcgg
     2341 tgtagccggc cagcacgcag ccgtagccgc gaaagtcgcg gtagcgcgcc gcaccggcgg
     2401 tgcacacgcc gatccacagc gacaggcaga gcaggaacag cacccgctcc tgggcgaaca
     2461 gggcgatcag cggcaccgtc accgtcagcc ccagcagggt gccgaggatg cggtagaagc
     2521 tcttggccag cacctggccg ctctccggct gcatgacgat gaacaccgtg gccagcacgg
     2581 tgttggcctg cggcagttcc aggcgatagg ccagccacag ggcgatgaag gcggtgagca
     2641 gggccttgaa gatgaaggcc cagatcatcc cctcgctctg tgcccattcg tccaggccgc
     2701 ggcg
//
LOCUS       DATCUN010000309         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_90154, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000309 DATCUN010000000
VERSION     DATCUN010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90154"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..>1522
                     /locus_tag="VIO83_11780"
     CDS             15..>1522
                     /locus_tag="VIO83_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331992.1"
                     /translation="MKTARLMALTVCLAVATASPAWLYGADTPLNATAEKLGEVNFPV
                     SCSAEAQQEFNRAMALFHSFWFDPAKASFAKVLEHDPACGMAHWGIAIMSMGNPFTWA
                     ANPNAAKAGAPAVAEAQRVGAKSERENAYIGALALLLKDWQTSEFRPRALAYEKAMEG
                     VTRQYPEDDEARILYALALNITASPTDKTFANQLKAAAILEPLFKKYPNHPGVAHYLI
                     HTYDYAELAEKGLPYARAYAAIAPSVPHALHMPSHIFSRLGLWPEMVEGDRASYLAAK
                     AELKETTLGIGAYDALHAMDYMVFGQLQQAQDKATKALLDEAAAISKVNVENFVAAYA
                     FAAIPSRFALERGDWKQAAQLKLTPPDLAWHKFPQSEAILVFARGLGAARSGDVAAAH
                     KDVSRLETLKAALTAANNSYWAGQTEVQIQTVNAWIALADGRNDEALQSMRTAAEAEE
                     ASDKHPVTPGNVVPSRELLGEMLLITNQPTQALAEFERSLKRDPNRLRGLYGA"
BASE COUNT          288 a          533 c          485 g          216 t
ORIGIN      
        1 agtggaggtg acgaatgaaa accgcacgac tgatggcgct cacggtttgt ctggcagtgg
       61 ccacggcgag tccggcatgg ctgtacggcg cggacacccc gctgaatgcg accgccgaga
      121 agctcggcga ggtgaacttc ccggtctcct gcagtgccga ggcgcaacaa gaattcaacc
      181 gggcgatggc gctgttccat tcgttctggt tcgacccggc gaaggcctcg tttgccaagg
      241 tgctggaaca cgatcccgcg tgcggcatgg cgcactgggg catcgccatc atgtcgatgg
      301 gcaacccctt cacttgggcg gccaacccca acgccgccaa ggcgggcgcg ccagccgtgg
      361 cggaggccca gcgcgttggc gccaagagcg aacgggaaaa cgcctacatc ggcgcgctcg
      421 ccctgctcct caaggactgg cagacgagcg aattccgccc gcgggcgctg gcctacgaga
      481 aagcgatgga gggcgtgacc cgccaatatc ccgaggatga cgaagcccgg atcctctacg
      541 ccctcgcgct caacatcacg gcctcgccca ccgacaagac cttcgccaac cagctcaagg
      601 cggccgccat tctcgagccg ctgttcaaga aatatcccaa ccaccccggc gtggcgcact
      661 acctgattca cacctacgac tatgccgaac tggccgagaa aggcctgccc tatgcgcgcg
      721 cctacgccgc cattgccccc tcggtgccgc atgcgctgca catgccttcg cacatcttct
      781 cgcgtctcgg cctgtggccg gagatggtcg aaggcgaccg cgcctcgtac ctggcggcca
      841 aggccgaact caaggagacg acgctcggca tcggcgccta cgacgccctg cacgcgatgg
      901 actacatggt gttcggccag ctccagcagg cgcaggacaa ggccaccaag gcgctgctgg
      961 atgaagcggc ggccatcagc aaggtgaacg tggagaactt cgtcgccgcc tacgcgttcg
     1021 ccgcgattcc ctcgcgtttc gcgctggaac gcggcgactg gaagcaggcg gcgcagctca
     1081 agctcacccc tcccgatctt gcctggcaca agttcccgca gtcggaggcc atcctggtgt
     1141 tcgcccgcgg cctgggcgca gcgcgcagcg gcgacgtcgc cgccgcacac aaggatgtca
     1201 gccgcctgga gacgctcaag gccgccctga cggcggccaa caacagctac tgggcggggc
     1261 agactgaggt gcaaatccag accgtgaacg cctggatcgc cctggcggac gggcgcaacg
     1321 acgaggcgct gcaatcgatg cgcaccgcgg cagaggccga ggaggccagc gacaagcacc
     1381 ccgtcacgcc gggaaacgtg gtgccctcgc gcgagctgct gggcgagatg ctgctcatca
     1441 ccaaccagcc gacccaggcg ctggcggagt tcgaacgctc gctcaagcgc gatcccaatc
     1501 gcctgcgcgg cctgtacggc gc
//
LOCUS       DATCUN010000310         4221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_146730, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000310 DATCUN010000000
VERSION     DATCUN010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146730"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      62..288
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            412..2262
                     /gene="btuB"
                     /locus_tag="VIO83_11785"
     CDS             412..2262
                     /gene="btuB"
                     /locus_tag="VIO83_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor"
                     /protein_id="HEY8331993.1"
                     /translation="MNKVLLAPAAILLCGVSPFSRAAEPLSIAPLVVTATRTAQTAEQ
                     SLAAVTVIDRAEIERKQAASVPELLRSVPGVSLANNGGPGKSTSLFLRGTESDHLLVL
                     IDGIKVGSATSGSAAFQDLPVELIERIEIVRGPRSSLYGSEAIGGVIQIFTRKGTGAG
                     LKPFFSVGSGSHDSHSGSAGLSGRAGSSWFNLGVSASDTDGINVKQRSASGHEDDRDG
                     YRNLSASLRAGHRFANGLELDGSFLQADAHNDFDSTTGEDAYSDGKQKVAGLRARFEP
                     LEPWQVTLQAGRSEDKSDAFEDGAFYSRFDTRRDSLSWQNDVTLAAGHTLVLGVDHQH
                     DEVNGTKSYAEDSRDNDGYFAQYLGGAGRHDWQLALRRDDNQQFGDHETGSVAYGFAL
                     SDELRAYASYGTAFKAPTFNELYYPGYGNPDLQAEKSKSVELGLNGQPGWGHWSANVY
                     RTQIDDLISSIRVNGKSLAQGVDETRIRGLELQLGSELLGWQWNAGYSLMDAENRSQR
                     SSRSGISYYGKDLNRRPAQTVNLDIDRAFGRFSVGSTLYAQDSAYDDLENQDELSGYA
                     SLDLRGEYRLDDEWRLQARVSNLLDADYQTAAGYNQPGQAVYFTVRYQAL"
     gene            2319..2891
                     /locus_tag="VIO83_11790"
     CDS             2319..2891
                     /locus_tag="VIO83_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8331994.1"
                     /translation="MLDLSPRSRLAVGIALALLMAVTRGHHFGDLNLPSASWAVFFLA
                     GALLPARWAFAALFLEAVALDLVAITWGGVSDWCVTRAYGFLLPAYAVLWFAGRSYAR
                     VHLDGWATLGRLLSCVLASSFVSHLLSSGGFWFFSGRYPEPTLAGFLPRIAAYYPPSL
                     QALAFYLGIAALLLAAGHAWAQRSSVEVSR"
     gene            2888..3505
                     /gene="cobO"
                     /locus_tag="VIO83_11795"
     CDS             2888..3505
                     /gene="cobO"
                     /locus_tag="VIO83_11795"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY8331995.1"
                     /translation="MSTQDELARDERHKARMQRKKAVVDAKIAEASDERGLLLVNSGN
                     GKGKSSAAFGMLARTLGHGLKAGVVQFIKGAASTGEEAFFRRFPDEVRYFVMGEGFTW
                     ETQDRQRDIDKAEQAWAVARQLLQDPEIALVVLDELNIALKYGYLQLDTVLADLAARP
                     PLQHVVVTGRGALPGLIEAADTVSEIGLVKHAFQAGVKAQKGVEL"
     gene            3618..>4221
                     /locus_tag="VIO83_11800"
     CDS             3618..>4221
                     /locus_tag="VIO83_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019407264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEY8331996.1"
                     /translation="MNNPRHCPALLIAAPASGQGKTTVTAALARHHVNQGRRVRVFKC
                     GPDFLDPMILARACGAPVDSLDLWLVGEEECRRRLWQAAGEADLILIEGVMGLFDGTP
                     SAADLARRFGVPVLAVIDGAAMAQTFAAIAHGLASYQSDLPFAGVLANRVGSARHGEI
                     LHDCLPAHLRWFGALPRDAAIELPSRHLGLVQAAELADLDA"
BASE COUNT          650 a         1470 c         1413 g          688 t
ORIGIN      
        1 gctgctgcgc ttgcaactcg gctgccgttg cgaatttgac gcatcgctag caaccctcta
       61 accttcgcgc cctgcttcag gtgcccccca gccaaggctc ggcggggtga aacagggaag
      121 ccggtgcgtg tgcgaaatgt cgcacacaag gccggcgctg cccccgcaac ggtaggcgag
      181 tcaggatgtc gctataggcc actgtgctca ggcatgggaa ggcgcgactt cgggaacccc
      241 aggttccact cgcaagcccg gagaccggcc tgtcgcatcc acggtatcgc ggagggcgct
      301 acccgtcggg tgggcggctt tcctcatata tcccgtctgt tcgtcgtcat gtcctctgca
      361 tgcctcctgc aatcctgatc gcgcgggtga gcgcgatgca gagagtgagc gatgaacaaa
      421 gtccttctgg cgcctgcagc catcctgctg tgtggcgtat ctcccttttc ccgggcggcc
      481 gagccgctgt cgatcgcgcc tctggtggtg acggcgaccc gcaccgcgca gaccgccgag
      541 cagtcgctgg cggcggtgac ggtgatcgac cgcgcggaga tcgagcgcaa gcaggcggcc
      601 tccgtgcccg agctgttgcg cagcgtgccg ggtgtgtcgc tggccaacaa cggcggtccc
      661 ggcaagagca cctcgctgtt cctgcgcggg accgagtccg accacctgct ggtgctgatc
      721 gacggcatca aggtcggctc ggccaccagc ggcagcgctg ccttccagga cctgccggtg
      781 gagctgatcg agcgcatcga gatcgtccgc ggcccgcgct ccagcctgta cggttcggaa
      841 gccatcggcg gggtgatcca gatcttcacc cgcaagggca cgggggctgg cctcaagccg
      901 ttcttctcgg tcggcagcgg cagccacgat agccacagcg gcagcgccgg gctgtccggc
      961 cgcgccggct ctagctggtt caacctcggc gtctcggcca gcgacaccga tggcatcaac
     1021 gtcaagcaac gcagcgccag cggccatgag gacgaccgcg atggctaccg caacctgtcc
     1081 gcctcgctgc gcgccgggca ccgcttcgcc aatggtttgg agctggacgg cagcttcctg
     1141 caggccgacg cgcacaacga tttcgacagc accactggcg aggacgccta tagcgacggc
     1201 aagcagaagg tcgccggcct gcgcgcccgc ttcgagccac tggagccctg gcaggtgacc
     1261 ctgcaggccg ggcgcagtga ggacaagtcg gatgccttcg aggacggcgc cttctattcg
     1321 cgcttcgata cccgtcgcga ctcgttgtcc tggcagaacg atgtgaccct ggccgcaggc
     1381 cacaccctgg tgctgggcgt cgaccaccag cacgatgagg tcaacggcac caagagctac
     1441 gccgaggatt cgcgcgacaa cgacggctac ttcgcccagt acctcggcgg ggccggtcgt
     1501 cacgactggc agctggcgct gcgtcgcgac gacaaccagc agttcggcga ccacgagacc
     1561 ggcagcgtcg cctatggctt cgcactgagc gacgagctgc gcgcctatgc cagctatggc
     1621 accgcgttca aggcaccgac cttcaacgag ctgtattacc ccggctacgg caatcccgac
     1681 ctgcaggcgg agaagtcgaa aagtgtcgag ttgggcctca acggtcagcc cggctggggg
     1741 cactggtcgg ccaatgtcta tcgcacccag atcgacgacc tgatcagcag tattcgcgtc
     1801 aatggcaaga gcctggccca gggcgtggac gagacgcgca tccgcggtct cgagctgcag
     1861 ctcggtagcg agctgctcgg ctggcagtgg aatgccggct acagcctgat ggatgccgag
     1921 aaccgctccc agcgcagtag ccgcagcggc atctcctact acggcaagga tctcaaccgc
     1981 cgtccggcgc agacggtcaa tctcgatatc gatcgcgcct tcggccgctt cagcgtgggg
     2041 agcacgctct acgcccagga ctcggcctac gacgatctgg agaaccagga cgaactgtcc
     2101 ggctacgcca gcctcgacct gcgtggcgaa taccgtctcg acgacgagtg gcgcctgcag
     2161 gcacgggtga gcaacctgct cgacgccgac taccagactg ccgccggcta caaccagccg
     2221 gggcaggccg tgtacttcac cgtgcgctac caggcgctct gagtcaccgg cgccgtgcat
     2281 gcgcagggcg ccacaccgtt gtcatcagga gataccccat gctcgacctg tccccccgta
     2341 gccgcctcgc tgtgggcatc gccctggccc tgctgatggc cgtcacccgc ggccaccact
     2401 tcggcgacct caacctgccc agcgcctcct gggcggtgtt cttcctcgcc ggggcgctgc
     2461 tgccggcgcg ctgggcgttc gcggcgctgt tcctcgaggc ggtcgccctc gacctggtgg
     2521 cgatcacctg gggtggggtc agcgactggt gcgtgactcg cgcctacggc ttcctgctac
     2581 cggcctatgc cgtgctgtgg ttcgctggcc gcagctacgc ccgtgtgcac ctggatggct
     2641 gggcgaccct tggccgcctg ctgtcctgcg tgctggcttc cagcttcgtc agccacctgc
     2701 tgtccagcgg cggcttctgg ttcttctccg gccgctaccc cgagccgacc ctggccggct
     2761 tcctgccgcg cattgccgcc tactacccgc cgtcgctgca ggcgctggcc ttctacctcg
     2821 gcatcgcggc gctgctgctg gccgccgggc atgcctgggc gcagcgttcg agcgtcgagg
     2881 tgagccgatg agcactcagg acgaactggc ccgcgacgag cgccacaagg cgcgcatgca
     2941 gcgcaagaag gcggtggtcg acgccaagat cgccgaggcc agtgacgagc gcggcctgct
     3001 gctggtcaac agcggcaacg gcaagggcaa gagcagcgcg gccttcggca tgctggcgcg
     3061 caccctcggt cacggcctca aggccggcgt ggtgcagttc atcaagggcg cggcgagcac
     3121 cggcgaggag gcgttcttcc gccgcttccc cgacgaggtg cgctacttcg tgatgggcga
     3181 gggcttcacc tgggagaccc aggaccgcca gcgcgacatc gacaaggccg agcaggcctg
     3241 ggcggtggcc cgccagctgc tgcaggaccc cgagatcgcc ctggtggtgc tcgacgagct
     3301 gaacatcgcc ctcaagtacg gctacctgca gctggacacg gtactcgccg atctcgccgc
     3361 gcgcccgccg ctgcagcacg tggtggtcac cggccgcggc gcattgcccg ggctgatcga
     3421 ggcggccgac acggtcagcg agatcggcct ggtcaagcac gccttccagg ccggggtgaa
     3481 ggcgcagaaa ggcgtggagt tatgatgcaa gtgcttatcg cggctgccaa tgcgaatttt
     3541 ctcgcggcaa acgggtcatt caaggcaacc cggccgcaaa tggatgcgtg cctgtgtgtg
     3601 tttgcctgga ggctcccatg aacaaccctc gccactgtcc cgccctgctg atcgcggccc
     3661 ccgcttccgg gcagggcaag accacggtca ccgccgccct ggcccgccac catgtcaatc
     3721 agggccggcg cgtgcgggtg ttcaagtgcg gcccggactt tctcgatccg atgatcctcg
     3781 cccgcgcctg cggcgcgccg gtggactcgc tggacctgtg gctggtcggc gaggaggagt
     3841 gccggcggcg cctgtggcag gccgccggtg aggccgacct gatcctcatc gagggggtga
     3901 tgggcctgtt cgacggcacg ccctcggccg ccgacctggc gcggcgcttc ggcgtgccgg
     3961 tgctggcggt gatcgacggt gcggcgatgg cgcagacctt cgccgctatc gcccacggcc
     4021 tggccagcta ccagagcgac ctgccgttcg ccggggtgct ggccaaccgg gtcggcagcg
     4081 cgcgccatgg cgagatcctg cacgactgcc tgccggcgca tctgcgctgg ttcggcgcgc
     4141 tgccgcgcga tgccgccatc gagttgccca gccgccatct cggcctggtg caggccgccg
     4201 agctggccga tctcgatgcg c
//
LOCUS       DATCUN010000311         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_109844, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000311 DATCUN010000000
VERSION     DATCUN010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109844"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VIO83_11805"
     CDS             complement(<1..899)
                     /locus_tag="VIO83_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253599.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HEY8331997.1"
                     /translation="MDGIFLQQLINGLTLGAVYGLIAIGYTMVYGIIGMINFAHGEVY
                     MISAYLSAIGLALLSFFGLESFPLLIFATLLFTVCVTGLYGWVIERIAYKPLRNSTRL
                     APLISAIGISLILQNYVQISQGARQQGVPTLLDGAFRVAIGEGFLQISYTKVFILIAA
                     FAGMAVLTWVIRYTKLGRMCRATQQDRKMAQILGIDTDRVISAVFVIGAAMAALAGVL
                     ITMNYGTFDFYAGFVIGIKAFTAAVLGGIGSLPGAMLGGLLLGVAEAQFSGLVNTDYK
                     DVFSFSLLVLILIFRPQGLLGRP"
     gene            complement(1078..>1732)
                     /locus_tag="VIO83_11810"
     CDS             complement(1078..>1732)
                     /locus_tag="VIO83_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8331998.1"
                     /translation="FIVDTLKGARVAIIHDKDTYGQGIANAAKAQLNKRGVTEVLYEG
                     LTRGEKDFNALVTKLRGANVDVVYFGGLHPEAGPLLRQMREQGLNAHFVSGDGVVTDE
                     LVTTAGGPQYVKGAYMTFGADPRKIPEGQAVVEKLRAGGYEPEGYTLYAYASLQTMAA
                     AFNGVGSNDAEEASAWLKSHSVPTVMGSKAWDRKGDLKVSDYVLYQWDDQGKYHQLP"
BASE COUNT          301 a          555 c          594 g          282 t
ORIGIN      
        1 tggggccggc ccaggagtcc ttgcgggcgg aagatcagga tcagcaccag cagcgagaag
       61 ctgaacacgt ccttgtagtc ggtgttgacc aggccggaga actgcgcctc ggcgacgccg
      121 agcagcaggc cgccgagcat cgcgccgggc agcgagccga tgccaccgag caccgcggcg
      181 gtgaacgcct tgatgccaat gacgaagccg gcgtagaagt cgaaggtgcc gtagttcatg
      241 gtgatcagca cgccggccag cgcggccatg gcggcgccga tgacgaacac cgcgctgatc
      301 acccggtcgg tgtcgatgcc gaggatctgc gccatcttgc ggtcctgctg ggtggcccgg
      361 cacatgcggc cgagcttggt gtagcgaatc acccaggtca gcacggccat gccggcgaag
      421 gcggcgatca ggatgaacac cttggtgtag ctgatctgca ggaaaccctc gccgatggcg
      481 acgcggaagg cgccgtcgag cagggtcggc acgccctgct ggcgcgcgcc ctggctgatc
      541 tgcacgtagt tctgcaggat cagcgagatg ccgatggcgc tgatcaaggg cgccagccgg
      601 gtggagttgc gcagcggctt gtaggcgatg cgctcgatca cccagccgta caggccggtg
      661 acgcagacgg tgaacagcag ggtggcgaag atcagcagcg gaaaggattc caggccgaag
      721 aagctcagca gcgccaggcc gatggccgac agataggcgc tgatcatgta cacctcgccg
      781 tgggcgaaat tgatcatgcc gatgatgccg tagaccatgg tgtagccgat ggcgatcagc
      841 ccgtagaccg cgccgagggt cagcccgttg atcagctgct gcaggaagat gccgtccatg
      901 cgtgtcgcct cagggggaac ggtggggggc gcgccctcgc cgtggtgcgg acccagggcc
      961 cgccacccga gcaagagcgt cgccgcgagg tcgggcctgc agcaccgtat ggtggaaaag
     1021 acctgcggtc gttttccacc ctacggatcg gagatgcccg cctttaccag actcgcctca
     1081 cggcaactgg tggtacttgc cctgatcgtc ccactggtag agcacgtagt cgctgacctt
     1141 gaggtcgccc ttgcgatccc acgccttgct gcccatcacg gtcggcaccg aatggctctt
     1201 caaccaggcg ctggcctcct ccgcgtcgtt cgaaccgacg ccgttgaagg ccgccgccat
     1261 cgtctgcagc gaagcatagg cgtacagggt gtagccctcc ggctcgtagc cgccggcgcg
     1321 cagcttctcg accaccgcct gcccctcggg aatcttgcgc gggtcggcgc cgaaggtcat
     1381 gtaggcgccc ttgacgtact gcgggccgcc ggcagtggtg accagttcgt cggtgaccac
     1441 gccgtcgccg gagacgaagt gggcgttcag tccctgctcg cgcatctgcc gcagcagcgg
     1501 gccggcctcc ggatgcaggc cgccgaaata gaccacgtcg acattggcgc cacgcagctt
     1561 ggtgaccagc gcgttgaagt ccttctcgcc acgggtcagc ccctcgtaga gcacctcggt
     1621 gacgccgcgc ttgttgagct gggccttggc ggcattggcg atgccctgac cgtaggtgtc
     1681 cttgtcgtgg atgatcgcca cccgcgcgcc cttcagggtg tcgacgatga ag
//
LOCUS       DATCUN010000312         9323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_166712, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000312 DATCUN010000000
VERSION     DATCUN010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166712"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VIO83_11815"
     CDS             <1..244
                     /locus_tag="VIO83_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8331999.1"
                     /translation="QNNAGGKSRAPRDIGQRSRDSLLRELPLSEPAVRSSRETQPVIV
                     HKPSRSDRLPTAEQLEQLEQESRVRSEKPALLTRNK"
     gene            complement(309..947)
                     /locus_tag="VIO83_11820"
     CDS             complement(309..947)
                     /locus_tag="VIO83_11820"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEY8332000.1"
                     /translation="MNEILLRYRFRLLLVASGLLTLVMGWPAPPLWLEWGCLLGLVLA
                     GINTLRHKRLFQQLALLIGGINLLLLGLDQLIELPQALSDGLGLIAFYLLLCATLFHR
                     VTGERPVTKELLYGLCALYLQLGLAFAFAYQVVSGLVPGAFNSPDKLLLDDFVYYSLA
                     TLTTVGYGDIHAIAPAARLLAVAEAVMGVMFIALLVARSLALMHEEEDEEEL"
     gene            complement(1067..1642)
                     /locus_tag="VIO83_11825"
     CDS             complement(1067..1642)
                     /locus_tag="VIO83_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY8332001.1"
                     /translation="MLKKSLLALSLGSLLFGAQAMAADYKIDKEGQHAFVNFKISHLG
                     YSWLYGTFKDFDGSFSFDAAKPEESKVQVELKTASVDSNHAERDKHLRSADFLNVAKH
                     PTATFASTGVKSTGAGSADITGNLTLNGVTRPVVIAAKFVGEGDDPWGGYRAGFEGST
                     KLKLKDFDIQKDLGPASQEVELIISVEGVRQ"
     gene            complement(1669..2223)
                     /locus_tag="VIO83_11830"
     CDS             complement(1669..2223)
                     /locus_tag="VIO83_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY8332002.1"
                     /translation="MQWRNSARRYGLVSILLHWGVALAVFGLFGLGLWMVGLDYYSSW
                     YRRAPELHKGIGILLFLAMLLRLLWRLLSPPPAALASYSALTRRGAALVHGLLYLGLF
                     VLMLSGYLISTADGRAIEVFGWFSVPATLSGVDGQEDLAGTVHRWLAWALVGLAVLHA
                     LAALKHHFIDRDATLSRMLGRERT"
     gene            2415..3821
                     /locus_tag="VIO83_11835"
     CDS             2415..3821
                     /locus_tag="VIO83_11835"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEY8332003.1"
                     /translation="MSRNQQWMERDLAVLWHPCTQMKDHEQLPLIPIRRGEGVWLEDF
                     DGKRYLDAVSSWWVNVFGHANPRISARIKAQLDQLEHVMLAGFSHQPVIELSERLVRL
                     TPAGLEHVFYADNGSSGIEVALKMSFHYWRNCGQADKKRFVTLTNSYHGETVAAMSVG
                     DVALFTDTYKPLLLDTLKVPSPDCYHRPEGVSWEAHSRTMFAHMEQTLAEHHREIAAV
                     IVEPLIQGAGGMRMYHPIYLKLLREACDRYQVHLIHDEIAVGFGRTGTLFACEQAGIT
                     PDFLVLSKALTGGYLPMAAVLTTDTVYQAFYADYATLRAFLHSHTYTGNPLACAAALA
                     TLDIFEEDNVIANNRALAAHMGRACAHLAEHPHVAEVRQTGMALAIEMVQDKAGKTPY
                     PWQERRGMAVYQHALSRGALLRPLGSVVYFLPPYVITPEQIDFLAEVASEGIDRATRP
                     RVSVAVPAGAPAEFRDPG"
     gene            3927..4646
                     /locus_tag="VIO83_11840"
     CDS             3927..4646
                     /locus_tag="VIO83_11840"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019471551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY8332004.1"
                     /translation="MRLSRFFIDAPLALGRHALPEAQAHYIGRVLRLAAGDAVQLFDG
                     SGQEFVGELVEVGKKTVQVELREQFAGLAESPLRIHLGQGLSRGERMDWAIQKATELG
                     VAEITPLVSERCEVRLKDERADKRLAHWRQIAISACEQCGRSVLPTLHAPVVLADWLA
                     QTEAELKLVLHPVAEPLASHPRPQSLAFLIGPEGGLSEAEVQQAQRAGYHAARLGPRV
                     LRTETAPVVALSVAQQLWGDF"
     gene            complement(4729..5205)
                     /locus_tag="VIO83_11845"
     CDS             complement(4729..5205)
                     /locus_tag="VIO83_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY8332005.1"
                     /translation="MNQAPAAGTTPQTLSSLLLPLTDRTLLVPSVALAELINQRPAEP
                     RIGVPDWYLGDIVWRDLCLPLLSFETASSGTPPAAPAPGARIAVINALGGRPHLKFFA
                     LLVQGIPRPYKLDASLAPAGAPLAALELDSAQVEGGVARIPDLVELEQKLADIGLI"
     gene            complement(5234..6256)
                     /locus_tag="VIO83_11850"
     CDS             complement(5234..6256)
                     /locus_tag="VIO83_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY8332006.1"
                     /translation="MTERSSGRVAVIADTSLQRHVLQQTLLANGYQVLLNSDPLRLEG
                     EQLSACAPDLWLVDLAQLEDSPLIDQLLEQATVPVLFGEGQAPERHSEHYPRWERRLL
                     GKLKRLVGDPARAVGPSLEVLLNQGQRPARLNLPDSLASTPLAAGEPARQVWLLAASL
                     GGPEAVKEFLDALPGGLPLGFVYAQHIDASFEQRLPQAVGRHSQWPVNLARAGDWVRY
                     GEVVVAPIGQELGFDEDGRMRVGPRPWPEPYSPSIDQMMLNLAQQFPSCCGVIAFSGM
                     GSDGSAAAAYVLRKGGLIWTQTADSCACPSMPDSLREGGYSSFSASPRELAEALVARL
                     AEQYDQ"
     gene            complement(6249..>9323)
                     /locus_tag="VIO83_11855"
     CDS             complement(6249..>9323)
                     /locus_tag="VIO83_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8332007.1"
                     /translation="EAEAEAEAEAEAEAEAEATAEPAGFTPPAAEWPELAFDPVPEQP
                     PATSLGGGSQFAPAVQAHAVQAAEVVERDPELVQIFLEEGFDILDSASEALQQWIAEG
                     GKGAGLDALQRDLHTLKGGARMAEIAEVGDLAHELEFLYEGLADGRLPAERALFGLLQ
                     ESHDQLALMLEALRNDEALPDGEQQIEAISRFRRQAQQSADARQAAAFELPEPESALS
                     VEAPMELLLPTEPEQAAEPAAAPTGEARPEYVTPALSDSEVLPLFLEEAGDQLESLQA
                     ALRRWESSGAAPVDEFLRVLNTLKGGALLAGQQHLGALSEQLAQRLGEAGSASAELDD
                     LLPEVQAGYQRLLADVERLRGAGLVEEAAASSPTPLAPLAVKVEPLVQQLVATRLVPR
                     KVLPFVQRAQQEAAEAAAKRAPQELVKVPAELLEALVNLAGETSIFRGRVEQQVNDTA
                     FTLGEMEATIERVRDQLRRLDTETQAQILSRYQAVNERAGYEDFDPLEMDRYSQLQQL
                     SRALFESASDLLDLKETLTARNRDAETLLLQQARVNTELQEGLMRTRMVPFDRLVPRL
                     RRIVRQVAGELGKQVELVVHNAEGEMDRSVLEGMVAPLEHMLRNAVDHGIEPLDVRRA
                     AGKPDAGTIRLSLSREGGDILLTLADDGGGVNIAAVRRKAIERGLMAADADLSDYEVL
                     QFILEAGFSTAAKVTQISGRGVGMDVVSAGVKQLGGTVSIDSASGRGTRFLVRLPFTV
                     SVNRALMVLSGEDLYAIPLNTVEGIVRIPPYELEAYYQPNSPRFEYAGQQYQVKYLGE
                     LLNNGQRPKLNGQSLPLPVILVRSAEYATAVQVDSLAGSREIVVKSLGAQFSAVHSIS
                     GATILGDGRVVVILDLLAALRARHAQQLPRHAGAAQLEHESSKQRPPLVMVVDDSVTV
                     RKVTTRLLERNGMDVVTAKDGVDAIAQLQEIVPDVMLLDIEMPRMDGFEVATLVRHDE
                     RLKGVPIIMITSRTGDKHRERALAIGVNQYLGKPYQESVLLENILQLVPRHD"
BASE COUNT         1493 a         3270 c         3180 g         1380 t
ORIGIN      
        1 gcagaataac gccggcggca agagccgcgc gccgcgcgac atcggccagc gctcgcgcga
       61 cagcctgctg cgcgagctgc ccctgagcga gccggcggtg cgcagcagcc gcgagaccca
      121 gccggtgatc gtgcacaagc cctcgcgcag cgaccggctg cccaccgccg agcagcttga
      181 gcagctcgag caggaaagcc gcgtacgcag cgagaagccg gccctgctga cccgcaacaa
      241 gtaagcagcc gtcgcaacgc aaaacgccga ccccagggtc ggcgttttgc tgtgcgggcg
      301 ctgaaagact acagctcctc ctcgtcctct tcctcgtgca tcagtgccag gctgcgcgcc
      361 accagcaggg caatgaacat cacccccatc accgcctcgg ccacggccag cagccgcgcc
      421 gccggcgcga tggcatggat gtcgccgtag ccgaccgtgg tcagggtcgc caggctgtag
      481 tagacgaagt cgtccagcag cagcttgtcc ggactgttga acgcccccgg caccagcccg
      541 ctgaccacct ggtaggcgaa ggcgaaggcc aggcccagct gcaggtacag cgcgcacaag
      601 ccgtagagca gctccttggt caccggccgc tcgccggtga cgcgatggaa cagggtggcg
      661 cagagcagca ggtagaaggc gatcaggccc aggccgtcgc tcagcgcctg cggcagctcg
      721 atcagctggt cgaggccgag cagcagcagg ttgatcccgc cgatcagcag cgccagctgc
      781 tggaacaggc gcttgtggcg cagggtattg atcccggcca gcaccaggcc gagcaggcag
      841 ccccactcca gccacagcgg cggcgcgggc cagcccatca ccagggtcag caggccactg
      901 gcgaccagca gcaggcgaaa gcggtagcgc agcaggattt cgttcacacg ggccagccgg
      961 ggaggatgca gggagagcga gtctgcagaa ggccacccgc ggcgtccagt cgccccagca
     1021 acgacaacgc cgccccgagg agcggcgttg cggcagggtg cgcagcttac tggcgcacgc
     1081 cttccaccga gatgatcagc tcgacttcct gggacgccgg gccgaggtcc ttctggatgt
     1141 cgaagtcctt cagcttgagc ttggtgctgc cctcgaagcc ggcgcgatag ccgccccacg
     1201 ggtcgtcgcc ttcgccgacg aacttggcgg cgatcaccac cggcctggtc acgccgttga
     1261 gggtcaggtt gccggtgatg tcggcgctgc ctgcaccggt ggacttcacg ccggtcgacg
     1321 cgaaggtggc ggtcgggtgc ttggcgacgt tgaggaagtc ggcgctgcgc aggtgcttgt
     1381 cgcgctcggc gtggttggag tcgacgctgg cggtcttcag ctcgacctgg accttgctct
     1441 cctccggctt ggcagcgtcg aagctgaagc tgccgtcgaa gtccttgaag gtgccgtaca
     1501 gccagctgta gccgaggtgg ctgatcttga agttgacgaa ggcgtgctgg ccttccttgt
     1561 cgatcttgta gtcggcggcc atggcctgag cgccgaacag cagggaaccc aggctcaggg
     1621 cgagcaggga cttcttcagc atgggggaga ctccgttcag gggtggattc aggtgcgttc
     1681 gcggccgagc atgcggctca gggtcgcatc acggtcgatg aagtggtgct tgagcgccgc
     1741 cagggcatgc agcacggcca ggccgaccag cgcccaggcc agccagcggt ggacggtgcc
     1801 ggcgaggtct tcctggccgt ccacgccgct cagggtggcc ggcacgctga accagccgaa
     1861 cacctcgatg gcgcggccgt cggcggtgga gatcaggtag ccggagagca tcaggacgaa
     1921 caggccgaga tacagcaggc cgtgcaccag cgcggcgccg cggcgggtca gcgcgctgta
     1981 gctggccagc gccgcgggcg gcgggctgag cagacgccag agcaggcgca gcagcatggc
     2041 gaggaacagc aggatgccga tgcccttgtg cagctcgggc gcgcggcggt accagctgct
     2101 gtagtagtcg aggccgacca tccacagacc gaggccgaac aggccgaaca ccgccagggc
     2161 gacgccccag tggaggagaa tgctgaccag cccgtaacgc cgggcggaat tgcgccattg
     2221 catgagaagg attcccgtta aacgcgtgga gggaagacta tcaacatacc catcgatatg
     2281 aagcgcaaaa tttgtgtcaa aaagatccaa aatatcgata ccttgcccgg ccgtcaccgc
     2341 agcgtcctgc tcatcccggg gcggacggcg ctaaactgtg caccctgccc gatcccaccc
     2401 gaggagaacc gccgatgagc cgcaaccagc agtggatgga gcgcgacctt gccgtgctct
     2461 ggcacccctg tacgcagatg aaggatcacg agcagctgcc actgatcccg atccgccgcg
     2521 gcgagggcgt ttggctggag gacttcgacg gcaagcgcta cctggatgcg gtcagctctt
     2581 ggtgggtcaa cgtgttcggc catgccaatc cacgcatcag cgcgcggatc aaggcccagc
     2641 tcgaccagct cgagcacgtg atgctggccg gcttcagcca tcagccggtg atcgagttgt
     2701 ccgagcggct ggtacggctc accccggccg ggctggagca cgtgttctac gccgacaacg
     2761 gctcctcggg catcgaggtg gcgctgaaga tgagcttcca ctactggcgc aattgcggcc
     2821 aggcggacaa gaagcgcttc gtcaccctga ccaacagcta ccacggcgag accgtggcgg
     2881 ccatgtcggt gggcgacgtg gcgctgttca ccgacaccta caagccgctg ctgctggaca
     2941 ccctcaaggt gcccagcccg gactgctacc accgcccgga aggggtgagc tgggaggcgc
     3001 attcgcgcac catgttcgcc cacatggagc agaccctcgc cgagcatcac cgggagatcg
     3061 ccgcggtgat cgtcgagccg ctgatccagg gcgccggcgg catgcgcatg taccacccca
     3121 tctacctcaa gctgctgcgc gaggcctgcg accgctatca ggtgcacctg atccacgacg
     3181 agatcgccgt cggcttcggc cgcaccggca cgctgttcgc ctgcgagcag gccggcatca
     3241 ccccggactt cctggtgcta tccaaggcgc tcaccggcgg ctacctgccg atggccgcgg
     3301 tgctcaccac cgacaccgtg taccaggcgt tctacgccga ctacgccacc ctgcgcgcct
     3361 tcctgcactc gcacacctac accggcaacc cgctggcctg cgccgccgcc ctggccaccc
     3421 tggacatctt cgaggaggac aacgtcatcg cgaacaaccg ggcgctggcc gcgcacatgg
     3481 gccgcgcctg cgcccacctc gccgagcacc cgcatgtcgc cgaggtgcgc cagaccggca
     3541 tggccctggc catcgagatg gtccaggaca aggcgggcaa gacgccttac ccgtggcagg
     3601 agcggcgtgg catggcggtc taccagcacg cgctgtcgcg cggcgccctg ctgcgcccgc
     3661 tgggcagcgt ggtgtacttc ctgccgccct acgtgatcac ccccgagcag atcgacttcc
     3721 tcgccgaggt ggcgagcgaa ggcatcgacc gggccacccg cccccgcgtc agcgtggcgg
     3781 tgccggcggg cgcgccggcc gagttccgcg acccaggctg atctgcgggg gcacccgcgg
     3841 catcgcacga tgctgcggcc ggctgcggtg cactcggcgc acccggcggt taaactctgc
     3901 gctttccctg ctgaacgttc acgcccatgc gcctctcccg tttcttcatc gacgcccccc
     3961 tcgcccttgg ccgccatgcg ctgccggagg cccaggccca ctacatcggc cgcgtgctgc
     4021 gcctcgccgc cggcgatgcc gtgcagctgt tcgacggcag cggccaggag ttcgtcggcg
     4081 aactggtcga ggtgggcaag aagacggtgc aggtggagct gcgcgaacag ttcgccggcc
     4141 tggccgagtc gccgctgcgc atccacctgg gccagggcct gtcacgcggc gagcgcatgg
     4201 actgggcgat ccagaaggcc accgagctgg gcgtggccga gatcaccccg ctggtcagcg
     4261 agcgctgcga ggtgcgcctg aaggacgagc gggccgacaa gcgcctggcc cactggcggc
     4321 agatcgccat cagcgcctgc gagcagtgcg gacgctcggt gctgccgacc ctccatgcgc
     4381 ccgtcgtgct agccgactgg ctggcgcaga ccgaagccga gttgaagctg gtgctgcatc
     4441 cggtggccga accgctggcc agccatcccc gcccgcaatc cctggcgttc ctgatcggcc
     4501 cggagggcgg cctgtccgag gcggaggtgc agcaggcgca gcgcgccggc tatcacgccg
     4561 cccgcctcgg cccgcgggtg ttgcgcaccg aaaccgcacc ggtggtggcg ctcagcgtgg
     4621 cgcagcagct gtggggcgac ttctaggccg ccactgcggc cgccactgaa ccgcaaataa
     4681 acaggcccgc acgcggcggg cctgtttatt gctcagcggt cgcgcgcctc agatcaggcc
     4741 gatatccgcc agtttctgct ccagctcgac cagatcaggg attctcgcca cgcccccctc
     4801 cacctgtgcc gagtccagct ccagcgcggc cagcggtgcg ccggcaggcg ccaggctggc
     4861 gtcgagcttg tacggccgcg gaatgccctg caccagcagg gcgaagaact tcaggtgcgg
     4921 ccggccacca agggcgttga tcaccgcgat gcgcgcgccg ggcgccggcg ccgctggcgg
     4981 cgtgccactc gacgcggtct cgaaggacag cagcggcagg cacaggtcgc gccagacgat
     5041 gtcaccgaga taccagtccg gcacaccgat ccgcggctcg gccggacgct ggttgatcag
     5101 ctcggccagc gccacgctgg gcaccagcag ggtgcgatcg gtcaggggca gcaacaggct
     5161 gctgagggtt tgcggcgtag tgccggcagc gggggcttgg ttcatgcctg actcctgaaa
     5221 acggcggcgc ggatcactgg tcgtactgct cggccaaccg cgccaccagc gcttcggcca
     5281 actcgcgcgg cgacgcgctg aaactgctgt agccgccttc gcgcaggctg tccggcatgc
     5341 tcgggcaggc gcaactgtcg gccgtctggg tccagatcag gccgcccttg cgcagcacgt
     5401 aggcggccgc cgcactgccg tcgctgccca tgccgctgaa ggcgatgacg ccgcaacagg
     5461 acgggaactg ctgggccagg ttgagcatca tctggtcgat cgagggactg tagggctccg
     5521 gccacgggcg cggaccgacg cgcatgcgac catcctcgtc gaagcccagc tcctgcccga
     5581 tcggcgccac caccacctcg ccgtagcgca cccagtcgcc ggcacgggcc aggttgaccg
     5641 gccactggct gtggcgaccg acggcctgcg gcaggcgctg ctcgaacgag gcgtcgatgt
     5701 gttgggcata cacgaagccc agcggcaggc cgccgggcag cgcatcgagg aactccttga
     5761 ccgcttccgg accgcccagc gaagccgcca gcagccagac ctggcgcgcc ggctcgccgg
     5821 cagccagcgg cgtgctggcc agcgagtccg gcaggttgag ccgcgcgggg cgctgcccct
     5881 ggttgagcaa cacctccagg ctcgggccga ccgcccgggc cggatcgccc accaggcgct
     5941 tgagcttgcc gagcagccgc cgctcccagc gcgggtagtg ctcggagtgg cgctccggag
     6001 cctgaccctc gccgaacagc acgggcaccg tggcctgctc gagcaactgg tcgatcagcg
     6061 gcgaatcctc cagctgggcc agatcgacca gccacagatc cggcgcgcag gcgctcagct
     6121 gctcgccctc gaggcgcagc ggatcgctgt tgagcagcac ctgataaccg ttggcgagca
     6181 gggtctgctg cagcacatgg cgctgcaaag aggtatcggc gatgaccgcc acgcgaccgc
     6241 tgctacgttc agtcatggcg cggcaccagc tgcaggatgt tctccagcag caccgactcc
     6301 tgatacggct tgccgaggta ctggttgacc ccgatggcca gggcgcgctc gcggtgctta
     6361 tcgccggtcc gcgaggtgat catgatgatc ggcacccctt tgaggcgctc gtcgtgacgc
     6421 accagggtgg ccacctcgaa gccgtccatg cgcggcatct cgatgtccag cagcatgacg
     6481 tccgggacga tttcctgcag ctgggcgatg gcatccaccc cgtccttggc ggtcaccacg
     6541 tccatgccgt tgcgctccag cagacgggtg gtcaccttgc gcacggtgac cgagtcgtcg
     6601 accaccatga ccagcggcgg gcgctgcttg ctgctctcgt gctcgagctg cgcggcgccc
     6661 gcatgccgcg gcagctgctg ggcatggcgg gcacgcaggg cggccagcag gtcgagaatc
     6721 accaccaccc ggccatcgcc gagaatggtc gcgccggaga tgctgtggac ggcggagaac
     6781 tgcgcgccca ggctcttcac cacgatttcg cgcgaaccgg ccaggctgtc cacctgcacc
     6841 gccgtggcgt attcggcgga acgcaccaga atgaccggca gcggcaggct ctgcccgttc
     6901 agcttgggcc gctggccatt gttgagcagc tcgccgaggt acttgacctg atactgctgg
     6961 ccagcatatt cgaagcgcgg cgagttcggc tggtagtagg cctccagctc gtacggcggg
     7021 atgcgcacga taccttcgac ggtgttcagc gggatggcgt agaggtcctc gccggaaagc
     7081 accatcagcg cgcggttgac cgacacggtg aacggcaggc ggaccaagaa acgcgtgccc
     7141 cggccgctgg ccgagtcgat gctgaccgtg ccgccgagct gcttgacgcc ggcggacacc
     7201 acgtccatgc cgaccccgcg cccggagatc tgggtgacct tggcggcggt ggagaagccg
     7261 gcctcgagaa tgaactgcag cacctcgtag tccgacaggt cggcatcggc cgccatcagg
     7321 ccgcgctcga tggccttgcg gcgcaccgcg gcgatgttca cgccgccgcc atcgtcggcc
     7381 agggtcagca ggatgtcgcc accctcgcgg ctcaggctca ggcggatggt gccggcatcg
     7441 ggcttgcccg ccgcgcggcg cacgtccagc ggctcgatgc catggtcgac ggcgttgcgc
     7501 agcatgtgct ccagcggcgc caccatgcct tccagcacgc tgcggtccat ctcgccttcg
     7561 gcgttgtgca ccaccagctc gacctgcttg cccagctcgc cggccacctg ccggacgatg
     7621 cggcgcagac gcggcaccag gcgatcgaag ggcaccatgc gggtacgcat caggccttcc
     7681 tgcagctcgg tgttgacccg cgcctgctgc agcagcaggg tttccgcatc gcggttgcgc
     7741 gcggtgaggg tttccttgag gtcgagcaag tcggacgcgg actcgaacag cgcacgcgac
     7801 agctgctgca gctgcgagta gcggtccatt tccagcgggt cgaagtcctc gtagccagcc
     7861 cgctcgttga ccgcctgata gcgcgagaga atctgcgcct gggtctcggt gtccagacgc
     7921 cgcaactggt cgcgcacccg ctcgatggtc gcttccatct cgccgagcgt gaaggcggtg
     7981 tcgttcacct gctgctcgac ccggccgcgg aagatcgagg tctcgccggc gaggttgacc
     8041 agcgcttcga gcaactcggc cggcaccttg accagctcct gcggggcacg cttggcggcg
     8101 gcctcggccg cctcctgctg ggcccgctgg acgaagggca ggaccttgcg cggcaccagg
     8161 cgggtcgcga ccagttgctg caccagcggc tcgaccttga cggccagcgg cgcgagcggt
     8221 gtgggcgagg aagcggcagc ctcctcgacc agcccggctc cgcgcagccg ttcgacgtcg
     8281 gccagcaggc gctggtatcc ggcctgcact tcggggagca ggtcgtccag ttcagcgctc
     8341 gcgctgcccg cctcgcccag gcgctgcgcg agctgctcgc tcagcgcgcc gagatgctgc
     8401 tggccggcca gcagcgcacc gcccttgagg gtgttcagta cgcggaggaa ctcgtccacc
     8461 ggtgccgcac cgctgctttc ccagcgacgc agtgccgcct gcagggactc cagctgatcg
     8521 ccggcctctt cgaggaacag cggcaggact tcgctgtcgc tcagcgccgg ggtgacgtac
     8581 tcgggccgcg cctcgccagt cggcgccgcg gcgggctcgg cagcctgctc cggctcggtc
     8641 ggcagcagca actccatcgg cgcctcgacc gacagcgcgg actcgggctc gggcaactcg
     8701 aaggccgcag cctggcgcgc gtcggcgctc tgctgcgcct ggcggcggaa gcggctgatc
     8761 gcctcgatct gctgctcgcc gtccggcagc gcctcgtcgt tgcggagcgc ctcgagcatc
     8821 agcgccagct ggtcgtgact ttcctgcagc aggccgaaca gcgcgcgctc cgccggcagg
     8881 cggccgtcgg cgagtccttc gtagaggaac tccagctcgt gggccagatc gccgacttca
     8941 gcgatctcgg ccatccgcgc gccccccttg agggtgtgca gatcgcgctg cagggcgtcg
     9001 agaccggcgc ccttgccgcc ctcggcaatc cactgctgca acgcctcgct ggccgagtcg
     9061 aggatgtcga agccttcctc gaggaaaatc tgcaccagtt ccggatcacg ctcgacaacc
     9121 tcggcggcct ggacggcatg cgcctgcacg gccggggcga actgactgcc ccccccaagc
     9181 gacgtggccg gcggctgctc aggcacaggg tcgaaagcca gctccggcca ttcggccgcc
     9241 ggcggcgtga agccggcagg ctcggccgtg gcttcggctt cggcttcggc ttcggcttcg
     9301 gcttcggctt cggcttcggc ttc
//
LOCUS       DATCUN010000313         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_109966, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000313 DATCUN010000000
VERSION     DATCUN010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109966"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1854)
                     /locus_tag="VIO83_11860"
     CDS             complement(46..1854)
                     /locus_tag="VIO83_11860"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY8332008.1"
                     /translation="MRPHLLALALVAALAGCQPADAPTSGSTPAASQQAGDAAVDAAF
                     ADLSKRALDTWMQLSPISATQIGDHRYDAEIDDLSAAGQQKMLTAYKGLVADLDRIDV
                     SKLSRENQVDAAILRNQLQSEIWSAEVLQSSKWDPQIYNGVAGSAIYGLMAREFAPLP
                     ERLKSATARMEKLPQIFAQARENLDPARVPKIHAETVAKQNRGILSIVDTFITPHIGE
                     LPAEDGKRLQAAIDGLKKAVEEQQTWLDKTLVPNAKGDFRIGAELYDQKLKFALNSSL
                     SRAEIGERARAELKRVRQDMYGIAQTVLKDKPGAPALPANPSDEQQQAAIEAALELAY
                     ADKPARDKVVDDAKAALAQSTEFVRKHDLMTLPDSPVDIILMPEFQRGVAVAYCDSPG
                     PLDKNLKTFYAVSPIPDDWNDKQVDSFLREYNSRMIHLLSIHEGTPGHYLEGWHSGKF
                     PSTLRAVLRSGLFAEGWAVYTERMMQEQGYLDNDPLFHLVQLKFYLRTISNAILDQGV
                     HVDNWTREQAMQLMTHDAFQQESEASGKWVRAQLTSAQLPTYFVGAQEHFDTRKAVQD
                     KQGAKFNLKAYHDQMLSYGAPPVRFARQLMLDQPIE"
     gene            1967..>2152
                     /locus_tag="VIO83_11865"
     CDS             1967..>2152
                     /locus_tag="VIO83_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY8332009.1"
                     /translation="MEYQGSCHCGRIAFTVQADAPIKDVIDCNCSMCRRRGSLLWFAP
                     REAFQLGTDIADVATYQF"
BASE COUNT          318 a          688 c          736 g          410 t
ORIGIN      
        1 ggttcgcacc gggccgttgc ctgtctgacg aggaaccagg cgggtttact cgatcggctg
       61 gtccagcatc agctggcgcg cgaagcgcac cggcggggcg ccgtaggaca gcatctggtc
      121 gtggtaggcc ttcaggttga acttcgcgcc ctgcttgtcc tgcacggcct tgcgggtgtc
      181 gaagtgttcc tgcgcaccga cgaagtaggt cggcagctgg gccgaggtca gctgcgcgcg
      241 cacccacttg cccgacgctt cgctttcctg ctggaaggca tcgtgggtca tcagctgcat
      301 cgcctgctca cgcgtccagt tgtccacgtg cacgccctgg tccaggatcg cgttggaaat
      361 ggtgcgcagg tagaacttca gctgcaccag gtggaacagc ggatcgttgt ccaggtagcc
      421 ctgctcctgc atcatccgct cggtgtacac cgcccagcct tcggcgaaca ggcccgaacg
      481 cagcaccgcg cgcagcgtgg aggggaactt gcccgaatgc cagccttcca ggtaatggcc
      541 cggcgtgcct tcatggatgc tcagcaggtg gatcatgcgg ctgttgtatt cgcgcaggaa
      601 cgagtcgacc tgcttgtcgt tccagtcgtc gggaatcggc gagaccgcgt agaaggtctt
      661 caggttcttg tccagcgggc ccggcgaatc gcagtaggcc acggccacgc cgcgctggaa
      721 ctccggcatc aggatgatgt ccaccggcga atcgggcagg gtcatcaggt catgcttgcg
      781 cacgaactcg gtggactgcg ccagcgcggc cttggcatcg tcgaccacct tgtcgcgggc
      841 cggcttgtcg gcgtaggcca gttccagcgc tgcttcgatc gcggcctgct gctgttcgtc
      901 gctcgggttg gccggcagtg ccggggcgcc cggcttgtcc ttgagcacgg tctgcgcgat
      961 gccgtacatg tcctggcgca cgcgcttgag ttcggcgcgc gcgcgctcac cgatctcggc
     1021 gcgcgacagc gaggagttca aggcgaactt cagcttctgg tcgtacagct ccgcaccgat
     1081 gcggaagtcg cccttggcat tcggcaccag ggtcttgtcc agccaggtct gctgttcttc
     1141 aacggccttc ttcaagccat cgatggccgc ctgcaggcgc ttgccgtctt cggccggcag
     1201 ctcgccgatg tgcggggtga tgaaggtatc gacgatgctc aggatgccgc ggttctgctt
     1261 ggccacggtc tcggcgtgga tcttcggcac gcgggccggg tccaggtttt cgcgggcctg
     1321 ggcaaagatc tgcggcagct tctccatgcg cgcggtggcc gacttgaggc gctcgggcag
     1381 cggcgcgaat tcgcgtgcca tcaggccgta gatcgcgctg ccggccacgc cgttgtagat
     1441 ctgcggatcc cacttggacg actgcagcac ctcggcgctc cagatttccg actgcagctg
     1501 gttgcgcagg atcgccgcgt ccacctggtt ctcgcgcgag agcttgctga cgtcgatgcg
     1561 gtccaggtcg gcgacaaggc ccttgtaggc ggtcaacatc ttctgctggc cggcggcgct
     1621 caggtcgtcg atctccgcat cgtagcggtg gtcgccgatc tgggtggcgc tgatcggcga
     1681 cagctgcatc caggtatcca gcgcgcgctt ggacaggtcg gcgaaggcgg cgtcgaccgc
     1741 cgcatctccg gcctgctggc tggcggccgg ggtggaaccg ctggtcgggg catcggccgg
     1801 ctggcaaccg gcgagggcgg cgacgagggc gagggcaaga agatgcgggc gcatcggcat
     1861 tcctgtacgg ggtcaatcct cgagcatagg cccccggggc tgctttgtcc ccgtgccata
     1921 ggattaccat gggcgcatac aaggactctc cagcggagcg cggggcatgg aataccaggg
     1981 aagctgccat tgcggcagga tcgcattcac cgtgcaggcc gacgccccca tcaaggacgt
     2041 catcgactgc aactgctcga tgtgccgccg ccgcggcagc ctgctctggt tcgccccgcg
     2101 cgaggcgttc cagctgggca ccgatatcgc cgacgtggcg acctaccagt tc
//
LOCUS       DATCUN010000314         1872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_185810, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000314 DATCUN010000000
VERSION     DATCUN010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185810"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1535)
                     /locus_tag="VIO83_11870"
     CDS             complement(156..1535)
                     /locus_tag="VIO83_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8332010.1"
                     /translation="MNAFARRAASLLLVVISLPLSAFASTGQPTHEFKLDNGLKVIVR
                     EDHRAPVVVSQLWYRIGSSYERPGFTGLSHALEHMMFKGSAKLGPGESSRILRELGAE
                     ENAFTSADYTAYYQVLARDRLEIALEMEADRLAGLRLPADEFAREIEVIKEERRLRTE
                     DNPAALAWERFKTAAYPATGYRTPTIGWMSDLERMSVDDLRAWHNDWYSPNNAVLVVV
                     GDVSADQVKTLAQRHFAAIPARPLPAARAPLELPALGERRLTLKVKTQLPSLLMGFNV
                     PGLASADNPREVQALHLIAALLDGGYSARLATRLEREQELVSGASASYDPFTRGDSLF
                     VLSARPNVQQGKTLAQVEAGLWHELDTLKTQAPSAAEVARVQAQVIAGLVYERDSIAS
                     QATSIGQLETVGLSWQLLDQDLEALKAVTPADIQSAAQRLFQRERLTVAQVLPQDSAA
                     PLAEETRHE"
     gene            1661..>1872
                     /locus_tag="VIO83_11875"
     CDS             1661..>1872
                     /locus_tag="VIO83_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017903463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY8332011.1"
                     /translation="MFGSKDDKKTPDSGEKKGLFGWFRKKAQPEATEQPVAVEPEQVS
                     PAAVPTAEPLAVAVEVEVEQPVRAEPL"
BASE COUNT          272 a          591 c          713 g          296 t
ORIGIN      
        1 aggcgatcgg cgcggggttg gccggggcgg cgctgctggt ggaagccggc gtcgccgggg
       61 cgggagccgg gttgtccgac tcggccgccg gccggctgat gaacagcagc aggctgagca
      121 gggccagcag cgcggcgagc agcagcaggt ggcgcttatt catggcgggt ctcctcggcg
      181 agcggcgcgg cactgtcttg cggcagcacc tgcgccacgg tcaggcgttc gcgctggaac
      241 aggcgctggg cggcgctctg gatgtcggcg ggggtcaccg ccttgagggc ttcgaggtcc
      301 tggtcgagca gctgccagga caggccgacg gtttccagct ggccgatgct ggtggcctgg
      361 ctggcgatgg agtcgcgctc gtagaccagc ccggcgatca cctgggcctg cacccgcgcc
      421 acttccgcgg cgctcggcgc ctgggtcttg agggtgtcca gctcgtgcca gaggccggcc
      481 tcgacctggg cgagggtctt gccctgctgc acgttgggtc gcgcggagag gacgaacagg
      541 ctgtcgccgc gggtgaaggg atcgtaggaa gccgaggcgc cgctgaccag ctcctgctcg
      601 cgctccaggc gggtggccag gcgggcgctg tagccgccgt cgagcagggc ggcgatcagg
      661 tgcagggcct gcacctcgcg cgggttgtcg gcactggcca ggccgggcac gttgaagccc
      721 atcagcaggc tgggcagctg ggtcttgacc ttgagggtca gccgccgttc gccgagcgcc
      781 ggcagctcca gcggcgccct ggccgccggc agcggacggg ccgggatggc ggcgaagtga
      841 cgctgggcga gggtcttcac ctggtcggcg ctgacgtcgc cgaccaccac caggacggcg
      901 ttgttcgggc tgtaccagtc gttgtgccag gcgcgcaggt cgtccaccga catgcgctcg
      961 agatcgctca tccagccgat ggtcggcgtg cggtagccgg tggccgggta ggcggcggtc
     1021 ttgaagcgtt cccaggccag cgccgcggga ttgtcctcgg tgcgcaggcg gcgctcctcc
     1081 ttgatcacct cgatctcgcg ggcgaactcg tcggccggca ggcgcagacc ggccaggcgg
     1141 tcggcctcca tctccagggc gatttccagg cgatcacgcg ccagcacctg gtagtaggcg
     1201 gtgtagtcgg cgctggtgaa ggcattctcc tcggcgccca gctcgcgcag gatgcgcgag
     1261 gattcgccgg gaccgagctt ggcgctgccc ttgaacatca tgtgctcgag ggcgtgggac
     1321 aggccggtga agccgggccg ttcgtagctg gagccgatgc ggtaccacag ctgggagact
     1381 accaccggcg cgcgatggtc ctcgcgcacg atcaccttga ggccattgtc gagcttgaac
     1441 tcgtgggtcg gctggccagt gctggcgaag gcgctcagcg gcaggctgat gacgaccagc
     1501 agcaggctgg cggcgcggcg cgcgaatgca ttcatgaata gtgacctgtc agactgccgg
     1561 ccggttttgg cgccggcggg cgaggaggtg ctaggatacc caaccgattt tctggcggcc
     1621 acgtcagtgc gcagccgctc acttgagata caagccgttc atgtttggtt ccaaagacga
     1681 caagaagact ccggatagcg gagaaaagaa aggcctgttc ggctggttcc gcaaaaaagc
     1741 gcagcctgag gccaccgagc agcccgtcgc cgtcgagccc gagcaggtgt cgcccgccgc
     1801 cgtcccgact gccgaaccgc tggccgttgc ggtcgaggtt gaggtcgagc agccagtgcg
     1861 cgccgagccg ct
//
LOCUS       DATCUN010000315         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_110013, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000315 DATCUN010000000
VERSION     DATCUN010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110013"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1785
                     /locus_tag="VIO83_11880"
     CDS             <1..1785
                     /locus_tag="VIO83_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY8332012.1"
                     /translation="TAPPAGAPTPASSVAALKLDIDSLAVSNTRIDYHDAASGRQYSI
                     EGLDLRSGAIREGASIPLTLKGLVSSNQPQLQATSELAGNLRFDTALKRYLLEDVVLK
                     GEASGAPLGSKTLVFSAQGQLLVDLAAQVAEWNGLKLSTNQLHTLGELKVRELDKQPK
                     LEGGLSIAEFDLRAFLESIDQSLPPMADANTLRRVSLAAGLSGSPTGLTLQDLKLSVD
                     DSRFSGQLAVADFSRQSLRAQLKGDRLDLDRYLPPPSEAAEAASASRKAEVQKTAQSS
                     GAGNTPLPDKPTTHAWSEAPLLPLPVLRRLDAELAVNLGQLTLRKLPIDNARLDLKAQ
                     NGQLNLGELRGDLFDGRFTVSGSLDARSDTPQLQLAPKLDNIPLERLLQTLKPEEKPP
                     LRGSLQLDASLNARGNSEKALIDSLAGKASFVLDDGALADANLEQQLCRGIATLNRKT
                     LSSEFADKDTPLQELRGSLQLKNGVASNQDLRARIPGLTVNGKGDIDLRVLGLDYRLG
                     LTIDGDQRATPDPACQVNERYADIQWPLRCRGPLELGAKACRLDQDGLGKIAARMAGE
                     KLSEKLDEKLGDKVDPELKDALKNLFKR"
     gene            1782..2849
                     /gene="mutY"
                     /locus_tag="VIO83_11885"
     CDS             1782..2849
                     /gene="mutY"
                     /locus_tag="VIO83_11885"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY8332013.1"
                     /translation="MSERYFATRVLTWYDRHGRHDLPWQQAISPYRVWVSEIMLQQTQ
                     VATVLGYYERFMAALPSVQALAAASEDEVLHLWTGLGYYSRARNLHKTARIVVEQHGG
                     EFPRSVEALTELPGIGRSTAGAIASISMGLRAPILDGNVKRVLARFLAQDGYPGETRV
                     AAQLWDAAERLTPPTRVNHYTQAMMDLGATLCTRSKPSCLLCPLQDDCQAHRLGREKD
                     YPQPKPRKVLPQKHTLMPLLHDGDGAILLYRRPSSGLWGGLWSLPELAQRDELATLAA
                     RHGLRLGSEQPLAALSHTFSHFQLHIEPLLIAVEPHGHAVAEGDWLWYNLASPPRLGL
                     AAPVKKLLKRAAETIDPPASR"
     gene            2886..3158
                     /locus_tag="VIO83_11890"
     CDS             2886..3158
                     /locus_tag="VIO83_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008091489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEY8332014.1"
                     /translation="MTRMVMCRKYNQELPALDRQPFPGAKGEDIFNHISQKAWDEWQK
                     HQTMLINERRLNMMDAADRKFLQGEMDKFFAGEEYAQAEGYVPPSE"
     gene            3266..3341
                     /locus_tag="VIO83_11895"
     tRNA            3266..3341
                     /locus_tag="VIO83_11895"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3299..3301,aa:Phe,seq:gaa)
     gene            3370..3445
                     /locus_tag="VIO83_11900"
     tRNA            3370..3445
                     /locus_tag="VIO83_11900"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3403..3405,aa:Phe,seq:gaa)
BASE COUNT          660 a         1308 c         1092 g          454 t
ORIGIN      
        1 accgcgccac ctgccggcgc gccgacgccg gcgtcgagcg ttgcggcgct caagctggac
       61 atcgacagcc tggcggtgag caacacgcgc atcgactacc acgacgccgc cagcggccgg
      121 caatacagca tcgaaggcct cgacctgcgt agcggtgcga tccgcgaagg cgccagcatc
      181 cccctgaccc tcaagggcct ggtcagcagc aaccagccgc agctgcaggc caccagcgag
      241 ctcgccggca acctgcgctt cgataccgcg ctcaagcgct acctgctgga agacgtggtg
      301 ctcaagggcg aggcatccgg cgcgccgctc ggcagcaaga ccctcgtctt cagcgcccag
      361 ggccagctgc tggtcgacct cgccgcgcag gtcgccgaat ggaacggcct caagctcagc
      421 accaaccagc tccacaccct cggcgagctg aaggtgcgcg aactggacaa gcagcccaag
      481 ctcgaggggg gcctgtcgat tgccgagttc gacctgcgcg ccttcctcga gagcatcgac
      541 cagagcctgc cgccgatggc cgatgccaac acgctcaggc gcgtcagcct ggccgccggc
      601 ctcagcggca gcccgaccgg cctgaccctg caggacctca agctgagcgt ggacgacagc
      661 cgcttcagcg gccagctcgc cgtcgccgac ttcagccggc agagcctgcg cgcccagctc
      721 aagggcgacc gcctcgacct cgatcgctac ctgccgccac ccagcgaggc agccgaggcc
      781 gccagcgcca gccgcaaggc cgaagtgcag aagaccgccc agagcagcgg cgcgggcaac
      841 accccgctgc cggacaagcc gaccacccac gcctggagcg aggcgccgct gctgccgctg
      901 ccggtcctgc gccgcctgga tgccgaactg gccgtcaacc tcggccagct gaccctgcgc
      961 aagctgccga tcgacaacgc caggctcgat ctcaaggcac agaacggcca gctcaacctc
     1021 ggcgagctgc gcggcgacct gttcgacggg cgcttcaccg tcagcggcag cctcgatgcg
     1081 cgcagcgaca ccccgcaact gcaattggcg cccaagctcg acaacatccc gctggagcgc
     1141 ctgctgcaga ccctcaagcc ggaggaaaag cccccgctgc gcggcagcct gcaactggac
     1201 gccagcctca acgcccgcgg caacagcgag aaggcgctga tcgacagcct ggccggcaag
     1261 gccagcttcg tcctcgacga cggcgccctg gccgacgcca acctcgagca gcaactgtgc
     1321 cgcggcatcg ccaccctcaa ccgcaagacg ctgagcagcg agttcgccga caaggacacg
     1381 ccgctgcagg aactgcgcgg cagcctgcag ctgaaaaacg gcgtggccag caaccaggac
     1441 ctgcgcgcgc gcatccccgg cctgacggtg aacggcaagg gcgacatcga cctgcgcgtg
     1501 ctcggcctcg actaccgcct cggcctgacc atcgacggcg accagcgcgc gacgcccgac
     1561 ccggcctgcc aggtcaacga gcgctacgcc gacatccaat ggccgctgcg ctgccgcggt
     1621 ccgctggaac tgggcgccaa ggcctgccgc ctggaccagg acggcctcgg caagatcgcc
     1681 gccaggatgg ccggcgagaa actgagcgaa aaactcgacg aaaaactcgg cgacaaggtc
     1741 gacccggaac tcaaagatgc cctcaagaac ctgttcaaac gatgagtgaa cgctacttcg
     1801 ccacccgggt gctgacctgg tacgaccgcc acggccgcca cgacctgccc tggcagcagg
     1861 ccatcagccc ctaccgggtg tgggtctcgg aaatcatgct gcagcagacc caggtcgcta
     1921 ccgtgctcgg ctactacgag cgcttcatgg cggccctgcc cagcgtgcag gcgctggccg
     1981 ccgccagcga ggacgaggtg ctgcacctgt ggaccggcct cggctactac agccgcgccc
     2041 gcaacctgca caagaccgcg cggatcgtgg tcgagcagca tggcggcgag ttcccccgtt
     2101 cagtggaggc cctgaccgag ctgcccggca tcggccgctc caccgccggc gccatcgcca
     2161 gcatcagcat gggcctgcgc gcaccgatcc tcgacggcaa cgtcaagcgc gtgctggcgc
     2221 gcttcctcgc ccaggacggc tatcccggcg aaacgcgggt ggccgcgcag ctgtgggacg
     2281 ccgccgagcg cctgaccccg ccgacgcggg tcaaccacta cacccaggcg atgatggacc
     2341 tgggcgccac cctgtgcacc cgcagcaagc cgagctgcct gctctgcccg ctgcaggacg
     2401 actgccaggc gcatcgcctc ggccgcgaga aggactatcc gcagcccaag ccgcgcaagg
     2461 tcctgccgca gaaacacacg ctgatgccgc tgctgcacga cggcgacggc gccatcctgc
     2521 tctaccggcg gccgtccagc ggcctgtggg gcggcctgtg gagcctgccg gagctggccc
     2581 agcgcgacga actggccaca ctggccgcac gccatggcct gcgcctgggc agcgagcagc
     2641 ccctggcggc gctgagccac accttcagcc acttccagct gcacatcgag ccactgctga
     2701 tcgccgtcga gccgcacggc cacgccgtgg ccgaaggcga ctggctctgg tataacctcg
     2761 ccagcccgcc gcgcctgggg cttgccgctc cggtgaagaa gctgctcaag cgcgcggccg
     2821 aaaccatcga cccgccggcc agccgctgag gcagccgcgc cgccaccccc cgccaggaga
     2881 gcgcaatgac ccgcatggtg atgtgccgca agtacaacca ggaacttccc gccctcgacc
     2941 ggcagccctt cccgggcgcc aagggcgagg acatcttcaa ccacatctcg cagaaggcct
     3001 gggacgagtg gcagaagcac cagaccatgc tgatcaacga gcgccggctg aacatgatgg
     3061 atgccgccga tcgcaagttc ctgcagggcg agatggacaa gttcttcgcc ggcgaggagt
     3121 acgcccaggc ggaaggctac gtaccgccca gcgaatgacc cccggcaagc gcaacctttc
     3181 tcgctaagcg cttaaaaaaa ctgatttttt tgccgccgac agttgactct gcggcggcaa
     3241 agacggttta atgccggccg ttgttgccca ggtagctcag tcggtagagc aggggattga
     3301 aaatccccgt gtcggcggtt cgattccgtc cctgggcacc acttatttca ggtgcccaat
     3361 ccctgatatg cccaggtagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcggc
     3421 ggttcgattc cgtccctggg caccactgat tacgccaaaa agcctcagcc ccgtgctggg
     3481 gctttttgct ttccggacgg ccgcaaagcc gttc
//
LOCUS       DATCUN010000316         4341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_90636, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000316 DATCUN010000000
VERSION     DATCUN010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90636"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VIO83_11905"
     CDS             complement(<1..368)
                     /locus_tag="VIO83_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY8332015.1"
                     /translation="MTTRTYQGHAPQLGARAFVDPSAVVIGDVVLGADCSVWPLAVIR
                     GDMHRIRIGARTSIQDGSVLHITHAGPFNPDGYPLEIGDDVTVGHKVILHGCSIGSRV
                     LVGMGAIVMDGAVVEDEVVIG"
     gene            513..1505
                     /locus_tag="VIO83_11910"
     CDS             513..1505
                     /locus_tag="VIO83_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEY8332016.1"
                     /translation="MAHLRLLFDNFTLALLGVIALASLLPCSGSGAVLFEWLTRLAIA
                     LLFFFHGARLSRAAIIAGAGHWRLHLLVFACTFALFPLLGLALRPLLAPWLGAELYVG
                     ILYLCALPGTVQSAIAFTAMARGNLPAAVCSASASSILGIFLTPLLVSLLLGAEAGSG
                     STLEAIGRILVQLLLPFLAGHLARPWLAGWVDRYKAWLKYVDQGSILLVVYTAFSAAV
                     IGGLWRAVPGPALVLLTLACCLLLAIALLATTWLSRRLGFSVEDEIAIVFCGSKKSLA
                     TGAPMAQVLFAGGAVGAVLLPLMLFHQIQLMVCAVLAQRYARRAAAAEGSAALS"
     gene            1590..3686
                     /gene="prlC"
                     /locus_tag="VIO83_11915"
     CDS             1590..3686
                     /gene="prlC"
                     /locus_tag="VIO83_11915"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HEY8332017.1"
                     /translation="MIPPSARSNAVTATNPLLQDFDLPPYSAIRPEHVEPAVDQILAD
                     NRAAIAAILEHQGAAPDWNGLVLALDELNARLGRAWSPVSHLNAVCNSAELRSAYEAC
                     LPKLSAYYTELGQNRALYQAWHALADSPAAAGFEVAQQTILKHTLRDFHLSGIDLPEA
                     EQQRYGAIQMKLSELCSTFSNQVLDATQAWTKQIGDEAALAGLPDSAKAQMLQAAQAK
                     GLDGWLVTLEFPSYYAVMTYADDRALREELYTAYATRASDQGPNAGQHDNGPLMAEIL
                     DLRQELAKLLGFAHYAELSLATKMAESTEQVLGFLRDLAVRGKPFAEQDLAELRAFAA
                     ERGVSDLSSWDVGYFSEKLRHQRYEISQEQVRAWFPIDKVLSGLFAIVERLYGIQIEE
                     LKGFDTWHPDVRLFEITENGAHVGRFFFDLYARANKRGGAWMDGARDHWRNSAGERIS
                     PVANLVCNFTPPVATAAVNGRPALLTHDEVTTLFHEFGHGLHHLLTRVDYAGVSGING
                     VAWDAVELPSQFMENWCWESEGLALISGHFESGESLPQDLLDKMLAAKNFQSGLMMLR
                     QLEFSLFDFELHASHGDGRSVLEVLQAIRDEVSVMQPPAWNRFANSFSHIFAGGYAAG
                     YYSYKWAEVLSADAFSKFEEEGVLNPATGRAFREAILARGGSLEPMQLFVDFRGREPS
                     IDALLRHSGLTEEVAA"
     gene            3683..3967
                     /locus_tag="VIO83_11920"
     CDS             3683..3967
                     /locus_tag="VIO83_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HEY8332018.1"
                     /translation="MSEEILNPPVKRFIAGAVCPACEAMDTIQMWDEDGTPNRHCVTC
                     GYADTLDARGNSVPKELPTRVNVSALKPKVADPKVQTVQFFPNPKLKKPE"
     gene            complement(4037..>4341)
                     /locus_tag="VIO83_11925"
     CDS             complement(4037..>4341)
                     /locus_tag="VIO83_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY8332019.1"
                     /translation="LCVGAALRPQELRGEGPTLLGNSLARLLLMPLLAFGVARALALP
                     SMESTLLMLFFALPTAPTAYTLTRQLGGDGHLMAGLITLQTLLAALSLPLLLGWLG"
BASE COUNT          640 a         1526 c         1487 g          688 t
ORIGIN      
        1 ccgatcacca cttcgtcctc gaccaccgcg ccgtccatga cgatggcgcc catgccgacc
       61 agcacgcggc tgccgatgga gcagccgtgc aggatcacct tgtggccgac ggtgacgtcg
      121 tcgccgatct ccagcggata gccgtccggg ttgaacggcc cggcatgggt gatgtgcagc
      181 acgctgccgt cctggatgct ggtgcgcgcg ccgatgcgga tgcggtgcat gtcgccgcgg
      241 attaccgcca gcggccacac cgagcagtcg gcgccgagca ccacgtcgcc gatgaccacg
      301 gcgctgggat cgacgaaggc gcgggcgccg agttgcgggg catggccctg ataggttctg
      361 gtggtcacgc tggaggctcc ttggccggat tcattgtgcc gttctaatta ccacggccag
      421 cgtctgccgg cgactgtccg ctggcagggc tgacggcagg cgcgtcggca ggctaaggtg
      481 gccgcttcct gagttggcaa gccgagaccg ccatggccca cctgcgcctg ctgttcgaca
      541 acttcaccct cgccctgctc ggcgtgatcg ccctcgccag tctgctgccc tgtagcggca
      601 gcggcgccgt gctgttcgag tggctcaccc gcctggcgat tgccctgctg ttcttcttcc
      661 atggcgcgcg gctgtcgcgc gcggcgatca tcgccggcgc cggccactgg cggctgcacc
      721 tgctggtgtt cgcctgcacc ttcgccctgt tcccgctgct tggcctggcg ctgcggccgc
      781 tgctggcgcc ctggctgggg gcggagctgt acgtcggcat cctctacctg tgcgcgttgc
      841 cgggcacggt acagtcggcc atcgccttca ccgccatggc gcgcggcaac ctgcctgccg
      901 ccgtgtgcag cgcctcggcc tccagcatcc tggggatctt cctcaccccg ctgctggtga
      961 gcctgctgct gggcgccgag gccggcagcg gttcgaccct ggaggccatc ggccgcatcc
     1021 tggtgcagct gctgctgccg ttcctcgccg gccacctggc gcggccctgg ctggcgggct
     1081 gggtggaccg ctacaaggcc tggctgaaat acgtcgacca gggctcgatc ctgctggtgg
     1141 tgtacaccgc cttcagtgcg gcggtgatcg gcgggctgtg gcgggcggtg ccggggccgg
     1201 cgctggtgct gctgacgctg gcctgctgcc tgctgctggc catcgcgctg ctggccacca
     1261 cctggctcag ccggcggctg ggcttttccg tcgaggacga gatcgccatc gtgttctgcg
     1321 gctcgaagaa gagcctggcc accggcgcgc ccatggcgca ggtgctgttc gccggcggcg
     1381 cggtgggcgc ggtattgctg ccgctgatgc tgttccacca gatccagctg atggtctgtg
     1441 cggtgctggc gcagcgctat gcacggcgcg ccgcggcggc cgaggggagt gccgcgctgt
     1501 cctgaggcct gcgcggcctc ggcggctata tcggaccgat agccatcgcc caagctgcgt
     1561 tgtcggacat ttgtcattaa catgggccga tgattccccc gtctgcaagg tccaacgccg
     1621 tgactgcaac caatcccctg ctgcaagact tcgacctgcc gccctattcc gcgattcgcc
     1681 cggagcacgt cgagccagcc gtcgaccaga tcctcgccga caaccgcgcc gccatcgccg
     1741 cgatcctcga gcaccagggc gccgcgcccg actggaacgg cctggtgctg gcgctggacg
     1801 agctgaacgc gcgcctgggc cgcgcctgga gcccggtcag ccacctcaac gcggtgtgca
     1861 acagcgccga gctgcgtagc gcctacgagg cctgcctgcc caagctgtcg gcctactaca
     1921 ccgagctggg ccagaaccgc gcgctgtacc aggcctggca cgcactggcc gacagcccgg
     1981 cggcggccgg cttcgaggtg gcgcagcaga ccatcctcaa gcacaccctg cgcgacttcc
     2041 acctcagcgg catcgacctg ccggaagccg agcagcagcg ctacggcgcg atccagatga
     2101 agctgtccga gctgtgcagc accttctcca accaggtgct cgacgccacc caggcgtgga
     2161 ccaagcagat cggcgacgaa gccgcgctgg ccggcctgcc ggattcggcc aaggcgcaga
     2221 tgctgcaggc cgcccaggcc aaggggctgg acggctggct ggtgaccctg gagttcccca
     2281 gctactacgc ggtgatgacc tacgccgacg accgtgccct gcgcgaggag ctgtacaccg
     2341 cctacgccac ccgcgcctcc gaccaggggc cgaacgccgg ccagcacgac aacggcccgc
     2401 tgatggccga gatcctcgac ctgcgccagg agctggccaa gctgctgggc ttcgcccact
     2461 acgccgagct gtcgctggcc accaagatgg ccgagtccac cgagcaggtg ctcggcttcc
     2521 tcagggatct ggcggtgcgc ggcaagccgt tcgccgagca ggatctggcc gagctgcgcg
     2581 ccttcgccgc cgagcgcggc gtcagcgacc tctcgagctg ggacgtcggc tacttcagcg
     2641 agaagctgcg ccaccagcgc tacgagattt cccaggagca ggtgcgcgcc tggttcccga
     2701 tcgacaaggt gctgtccggg ctgttcgcca tcgtcgagcg cctgtacggc atccagatcg
     2761 aggagctcaa gggcttcgac acctggcacc cggacgtgcg cctgttcgag atcacggaaa
     2821 acggcgcgca cgtcggccgc ttcttcttcg acctctacgc gcgcgccaac aagcgcggcg
     2881 gcgcctggat ggacggcgcc cgcgaccact ggcgcaacag cgccggcgag cgcatcagcc
     2941 cggtggccaa cctggtgtgc aacttcactc cgccagtcgc tactgcggcg gtcaacggcc
     3001 ggccggcgct gctgacccac gacgaggtca ccaccctgtt ccacgagttc ggccacggcc
     3061 tgcaccacct gctgactcgc gtcgactacg ccggcgtctc cggcatcaac ggcgtggcct
     3121 gggacgccgt cgagctgccc agccagttca tggagaactg gtgctgggag tcggaaggtc
     3181 tggcgctgat ctccggccac ttcgagagcg gcgagtcgct gccgcaggac ctgctggaca
     3241 agatgctggc ggcgaagaac ttccagtccg ggctgatgat gctgcgccag ctggagttct
     3301 cgctgttcga cttcgagctg cacgccagcc atggcgacgg ccgcagcgtg ctcgaggtgc
     3361 tgcaggcgat ccgcgacgag gtgtcggtga tgcagccgcc ggcctggaac cgcttcgcca
     3421 acagcttctc gcacatcttc gccggcggtt acgcggccgg ctactacagc tacaagtggg
     3481 ccgaggtgct gtcggccgac gccttctcca agttcgagga agagggcgtg ctcaacccgg
     3541 ccaccggccg tgccttccgc gaggcgatcc tcgcccgcgg cggctcgctg gagccgatgc
     3601 agctgttcgt cgacttccgc ggccgcgagc cgtcgatcga cgccctgctg cgccatagtg
     3661 gcctgaccga ggaggtggca gcatgagcga ggaaatcctc aatccgccgg tgaaacgctt
     3721 catcgccggc gcggtgtgcc cggcctgcga ggcgatggac accatccaga tgtgggacga
     3781 ggacggcacg cccaatcgcc actgcgtgac ctgcggctac gccgataccc tcgacgcccg
     3841 cggcaactcg gtgcccaagg agctgccgac gcgggtcaac gtcagcgcgc tgaagccgaa
     3901 ggtggccgat cccaaggtgc agaccgtgca gttcttcccc aatccgaagc tgaagaagcc
     3961 ggagtagcgc gggaaggtcc tgccgtgacg gacaaggcgc ccaccgcaag gtcggcgcct
     4021 ttttcgtgcc tgcgcttcag cccagccagc cgagcagcag cggcaggctg agggcggcga
     4081 gcagggtctg cagggtgatg aggccggcca tcaggtggcc gtcgccgccg agctggcggg
     4141 tcagggtgta ggcggtgggc gcggtgggca gggcgaagaa cagcatgagc agggtgcttt
     4201 ccatgctcgg cagcgccagc gcgcgggcca cgccgaaggc cagcagcggc atcagcagca
     4261 ggcgcgccag gctgttgccg agcagcgtcg ggccttcgcc gcgcagctcc tgcgggcgca
     4321 gcgcggcgcc gacacagagc a
//
LOCUS       DATCUN010000317         4715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_54210, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000317 DATCUN010000000
VERSION     DATCUN010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54210"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VIO83_11930"
     CDS             <1..513
                     /locus_tag="VIO83_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEY8332020.1"
                     /translation="LAPRYVEFIGKEIQTAYLESYSEYGQNIFDRYVLYADFWIQDQE
                     YRDPETGEILNRGSLNEELEKIEKPAGISNPKDFRNEIVNFVLRARANNNGKNPSWLS
                     YEKLRAVIEKKMFSNTEELLPVISFNAKGSKDEQQKHNDFVRRMVERGYTEKQVRLLA
                     EWYLRVRKSQ"
     gene            599..1873
                     /locus_tag="VIO83_11935"
     CDS             599..1873
                     /locus_tag="VIO83_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HEY8332021.1"
                     /translation="MSYVIDRRLNGKNKSTVNRQRFLRRYRDHIKKAVEEAVSRRSIT
                     DMEHGEQISIPSRDIDEPVWHHGRGGRQTIIHPGNKEFISGERIPRPSGGGGGGAGQG
                     KASNSGEGMDEFVFQITQEEFLDFMFEDLALPNLVKKHLAGMDSFKTVRAGISSEGTP
                     ARLNIVRTLRSAHARRIALSGSSRARLRELKAELERLRIEEPDNFTDIRALEEEIGGL
                     RARIERLPFLDTFDLKYNLLVKQPNPTSKAVMFCLMDVSGSMTQATKDIAKRFFILLY
                     LFLKRSYDKIEVVFIRHHTSAKEVDEQEFFYSRETGGTIVSSALKLMQEVMAARYPVN
                     EWNIYAAQASDGDNWNDDSPVCRDILMRQIMPFVQYFSYVEITPREHQALWHEYEQVS
                     EAFPESFAQQQIVSAGDIYPVFRELFQRRVAA"
     gene            1870..3429
                     /locus_tag="VIO83_11940"
     CDS             1870..3429
                     /locus_tag="VIO83_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEY8332022.1"
                     /translation="MNPRAKKGQPLSTGSEWTFELIRTYDREIARLAERYALDTYPNQ
                     IEVITAEQMMDAYASVGMPIGYHHWSYGKHFLSTEKGYKRGQMGLAYEIVINSDPCIA
                     YLMEENTMCMQALVVAHACYGHNSFFKGNYLFRTWTDARSIIDYLVFARQYIDKCEER
                     HGIDAVEELLDSCHALMNYGVDRYKRPYPISAEEERQRQKEREEQLQQQVNDLWRTIP
                     KGAGKGRDDDGKRFPAEPQENILYFIEKHAPLLEPWQREVVRIVRKVAQYFYPQRQTQ
                     VMNEGWATFWHYTLLNDLYDEGLVTDGFMMEFLQYHTSVVYQPPFDSPHYSGINPYAL
                     GFAMYRDIRRICEEPTEEDRRWFPDIAGSDWLATLKFAMQNFKDESFIQQFLSPKLMR
                     EFRLFSILDDDQQEELLVDAIHDDNGYRTIRENLAAQYNLGNREPNIQIWSVDRRGDR
                     SLTLRHFQHERKPLGNSTEDVLRHLHRLWGFDVHLESMQGEQRTAVQHMPPRREREAE
                     GSHFDLIARPI"
     gene            3505..>4715
                     /locus_tag="VIO83_11945"
     CDS             3505..>4715
                     /locus_tag="VIO83_11945"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEY8332023.1"
                     /translation="MQIYKVGGAVRDRLLGRPVTETDWVVVGAHAEELLAQGFRPVGA
                     DFPVFLHPQSGEEYALARTERKSGRGYGGFTFYASPEVTLEEDLIRRDLTINAIAEDA
                     DGRLTDPYGGQHDLALHLLRHVSPAFAEDPLRVLRVARFAARYAPLGFRVAEETQALM
                     AEIAVSGELAELTAERVWKEIARALMEPRPEVFLQVLRDCGALQQLLPEVQALFGVPQ
                     PALHHPEIDTGEHVLMVLRQCAEHGQPLAVRWACLLHDLGKGLTDPQEWPRHIAHEAR
                     GLRLIETVNARCKAPRDCAELALLVGEYHTHAHRARELRAQTLLELFQHFDLYRRPER
                     FEAFIAASEMDARGRRGLEQQPYPQADYLRAAAGVARGVGVQALLEQGFTGAELGEAL
                     KRERLRALKAFR"
BASE COUNT          904 a         1657 c         1471 g          683 t
ORIGIN      
        1 ctggcgccgc gctacgtcga gttcatcggc aaggaaatcc agaccgccta cctggagtcc
       61 tacagcgagt acgggcagaa catcttcgac cgctacgtgc tgtacgccga cttctggatc
      121 caggaccagg agtaccgcga cccggagacc ggcgagatcc tcaaccgcgg ctcgctcaac
      181 gaggagctgg agaagatcga gaagccggcc ggcatcagca acccgaagga tttccgcaac
      241 gagatcgtca acttcgtgct gcgcgcccgg gccaacaaca acggcaagaa cccgtcgtgg
      301 ctgtcctacg agaagctgcg cgcggtgatc gagaagaaga tgttctccaa caccgaggag
      361 ctgctgccgg tcatcagctt caacgccaag ggcagcaagg acgagcagca gaagcacaac
      421 gacttcgtgc ggcgcatggt cgagcgcggc tacaccgaga aacaggtgcg cctgctcgcc
      481 gaatggtacc tgcgcgtgcg caagtcgcag tagtaatcta gggaaggccc ggcgcggagc
      541 gagaaccatc ccgctccgcg ccttgcgctc cgggtccaag accgcacggg agtacgccat
      601 gagttacgtc atcgaccggc gactgaacgg caagaacaag agcacggtga accgccagcg
      661 cttcctgcgg cgttaccgcg accacatcaa gaaggcggtg gaagaagccg tcagccgccg
      721 ctccattacc gacatggaac acggcgagca gatcagcatc ccctcacgcg acatcgacga
      781 gccggtgtgg caccacggcc gcggcggccg gcagaccatc atccacccgg gcaacaagga
      841 gttcatcagc ggcgagcgca tcccgcgccc ctccggcggc gggggcggcg gcgcgggcca
      901 gggcaaggcc agcaattccg gcgagggcat ggacgaattc gtgttccaga tcacccagga
      961 ggagttcctc gacttcatgt tcgaggacct ggcgctgccc aacctggtga agaagcacct
     1021 ggccggcatg gacagtttca agaccgtgcg cgccgggatc agcagcgagg gcacccccgc
     1081 gcgactgaac atcgtgcgca ccctgcgctc ggcccatgcc cggcgcatcg ccctcagcgg
     1141 ctcgagccgc gcgcgcctgc gcgagctcaa ggccgagctg gaacggctgc gcatcgagga
     1201 gccggacaac ttcacggata tccgcgccct ggaggaagaa atcggcggcc tgcgggcacg
     1261 catcgagcgc ctgccgttcc tcgatacctt cgacctcaag tacaacctgc tggtcaagca
     1321 gcccaacccg acctccaaag cggtgatgtt ctgcctgatg gacgtgtccg gctcgatgac
     1381 ccaggccacc aaggacatcg ccaagcggtt cttcatcctg ctgtacctgt tcctcaagcg
     1441 cagctacgac aagatcgagg tggtgttcat ccgccaccac accagcgcca aggaggtcga
     1501 cgagcaggag ttcttctact cgcgggaaac cggcggcacc atcgtctcca gcgcgctcaa
     1561 gctgatgcag gaagtcatgg ccgcccgcta cccggtcaac gaatggaaca tctacgccgc
     1621 ccaggcctcg gacggcgaca actggaacga cgactcgccg gtctgccgcg acatcctgat
     1681 gcgccagatc atgccgttcg tgcagtactt cagctatgtc gaaatcaccc cgcgcgagca
     1741 ccaggcgctg tggcacgagt acgagcaggt cagcgaagcc ttcccggaga gcttcgccca
     1801 gcagcagatc gtctccgccg gcgacatcta cccggtgttc cgcgaactgt tccagaggag
     1861 ggtcgccgca tgaaccctcg cgccaagaaa ggccagccgc tgtccaccgg ctcggaatgg
     1921 accttcgagc tgatccgcac ctacgaccgc gagatcgccc ggctggccga acgctatgcg
     1981 ctggacacct atcccaacca gatcgaggtg atcaccgccg agcagatgat ggatgcctac
     2041 gcctcggtgg gcatgccgat cggctaccac cactggtcct acggcaagca cttcctcagc
     2101 accgagaagg gctacaagcg cgggcagatg ggcctggcct acgagatcgt gatcaactcc
     2161 gacccgtgca tcgcctacct catggaagag aacaccatgt gcatgcaggc cctggtggtg
     2221 gcccatgcct gctacggtca caacagcttc ttcaagggca actacctgtt ccgcacttgg
     2281 accgacgccc gctcgatcat cgactacctg gtgttcgccc gccagtacat cgacaagtgc
     2341 gaggagcgcc acggcatcga cgcggtcgag gagctgctgg attcctgcca cgcgctgatg
     2401 aactacggcg tcgaccgcta caagcggccc tacccgatct ccgccgagga ggaacgccag
     2461 cgccagaagg aacgcgagga acaactgcag cagcaggtca acgacctgtg gcgcaccatc
     2521 cccaaggggg ccggcaaggg ccgcgacgac gacggcaagc gctttccggc cgagccgcag
     2581 gagaacatcc tctacttcat cgagaagcac gccccgctgc tcgagccctg gcagcgcgag
     2641 gtggtgcgca tcgtgcgcaa ggtcgcccag tacttctacc cgcagcggca gacccaggtg
     2701 atgaacgagg gctgggccac cttctggcac tacaccctgc tcaacgacct gtacgacgag
     2761 gggctggtca ccgatggctt catgatggag ttcctccagt accacaccag cgtggtctac
     2821 cagccgccgt tcgacagccc gcactacagc ggcatcaacc cctacgcgct gggcttcgcc
     2881 atgtaccgcg acatccggcg catctgcgag gagccgaccg aggaggatcg ccgctggttc
     2941 cccgacattg ccggcagtga ctggctggcc acgctgaagt tcgccatgca gaacttcaag
     3001 gacgagagct tcatccagca gttcctctcg cccaagctga tgcgcgaatt ccgcctgttc
     3061 agcatcctcg acgacgacca gcaggaggag ctgctggtgg atgccatcca tgacgacaat
     3121 ggctaccgca ccatccgcga aaatctggcc gcgcagtaca acctcggcaa ccgcgagccg
     3181 aacatccaga tctggagcgt cgaccgccgc ggcgaccgct cgctgacgct gcgccacttc
     3241 cagcacgagc gcaagccgct gggcaactcc accgaggatg tgctcaggca cctgcaccgc
     3301 ctgtggggct tcgatgtgca cctggaaagc atgcagggcg agcagcgcac ggccgtgcag
     3361 cacatgccgc cgcgccgcga gcgcgaggcg gaaggcagcc acttcgacct gatcgcccgc
     3421 ccgatctgac ctccgcgccg ccatcgcccc gcgggcggtg gcgcgttatc ctctgcgcaa
     3481 cttcagttgc ccgcaggaca tcccatgcag atctacaagg tgggcggcgc cgtacgcgac
     3541 cgcctgctcg gccgccccgt cacggaaacc gactgggtgg tggtcggcgc ccacgccgag
     3601 gaactgctcg cccagggctt tcgcccggtc ggcgccgact tcccggtgtt cctccacccg
     3661 cagagcggcg aggaatacgc cctcgcccgc accgagcgca agagcggacg gggctacggc
     3721 ggcttcacct tctacgccag tcccgaggtc accctcgagg aagatctgat ccgccgcgac
     3781 ctgaccatca atgccatcgc cgaggacgcc gacggccggc tgaccgatcc ctacggcggc
     3841 cagcacgacc tcgccctgca cctgttgcgc cacgtctcgc cggccttcgc cgaggacccg
     3901 ctgcgcgtgc tgcgcgtcgc ccgcttcgcc gcgcgctacg cgccgctggg ctttcgcgtg
     3961 gccgaggaaa cccaggcgct gatggccgag atcgccgtct ccggcgagct ggccgagctg
     4021 accgccgagc gggtctggaa ggaaatcgcc cgcgcgctga tggagccacg cccggaagtg
     4081 ttcctccagg tgctgcgcga ctgcggcgcc ctgcagcagc tgctgcccga ggtgcaggcg
     4141 ctgttcggcg tgccgcagcc ggcgctgcac cacccggaga tcgacaccgg cgagcatgtg
     4201 ctgatggtcc tgcgccagtg cgccgagcat ggccagccac tggcggtgcg ctgggcctgc
     4261 ctgttgcatg acctcggcaa gggcctcacc gatccgcagg agtggccgcg gcatatcgcc
     4321 catgaggcgc gcggcctgcg cctgatcgag acagtcaacg cccgctgcaa ggcgccgcgc
     4381 gactgcgccg aactggccct gctggtcggc gaataccaca cccacgcgca ccgcgcccgg
     4441 gagttgcgcg cgcagaccct gctggagctg ttccagcact tcgacctcta ccgccggccg
     4501 gagcgcttcg aggccttcat cgccgccagc gagatggatg cccgcggccg ccgcggcctc
     4561 gaacagcagc cctacccgca ggcggactac ctgcgcgcgg ccgccggcgt ggcacgtggc
     4621 gtcggcgtgc aggcgctgct cgagcagggt ttcacgggcg cggaactggg cgaggcactc
     4681 aagcgcgagc gactgcgggc gctcaaggct ttccg
//
LOCUS       DATCUN010000318         9181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_147269, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000318 DATCUN010000000
VERSION     DATCUN010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9181
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147269"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VIO83_11950"
     CDS             <1..635
                     /locus_tag="VIO83_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017906824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta
                     transmembrane domain-containing protein"
                     /protein_id="HEY8332024.1"
                     /translation="MHCADPGCLKACPSPGAIVKYANGIVDFNQDKCIGCGYCITGCP
                     FDVPRISQKDHKAYKCTLCSDRVAVGLEPACVKTCPTGAIVFGSKADMQEHAAGRIAD
                     LKERGFDQAGLYDPDGVGGTHVMYVLHHADQPSLYAGLPNAPAISLLVSLWKGITKPL
                     ALLAMGATVLAGFFHYVRIGPNRVDEEEDPGEPTVHQVDPTVHVVDPNKP"
     gene            719..1342
                     /locus_tag="VIO83_11955"
     CDS             719..1342
                     /locus_tag="VIO83_11955"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEY8332025.1"
                     /translation="MNNEIQRYTPSERSNHWAVAILFFLAGLSGLALFHPALFGLSGL
                     FGGGVWTRILHPFLGLAMFALFLIMAMRFAGHNRLNANDRQWLGQMGDVLNNREDKLP
                     EVGRYNAGQKLLFWVLVLCMLVLLASGIVIWRQYFGQLFGVGLIRLAAVAHALAAFVL
                     ITSIIVHIYAAIWVKGSVGAMLTGKVSRAWARKHHPGWFKDVERQRK"
     gene            1823..3976
                     /locus_tag="VIO83_11960"
     CDS             1823..3976
                     /locus_tag="VIO83_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY8332026.1"
                     /translation="MDMQRSKVQVMGIDAGGTMTDTFFVREDGNFVVGKAQSNPADES
                     LAIYNSSLDALSTWGQGVDDVYPELVTCVYSGTAMLNRVVQRKGLDVGLIVNRGFEQT
                     HSMGRAIQSYLGYALEERLHLNTHRYDEPLVPYSRTRGVTERTDVQGKVVIPLREEEV
                     RQATRELVEAGAQAIAIVLLQSHKNASSEKRARDVCREELQRLGSEIPVFASVDYYPS
                     RKESHRTNTTILEAYAAEPSRQTLAKVSERFKKHGAKFDLRVMATHGGTISWKAKELA
                     RTIVSGPIGGVIGSRLLGEALGYENIACSDIGGTSFDMALITKGNFAIASDPDMARLV
                     LSLPLVAMDSVGAGAGSFVRLDPYSGSIKLGPDSAGYRVGTCWPESGLDTVSVTDCHV
                     VLGYLNPDNFLGGAIKLDVERARRHIQEQIAGPLGLSVEDAAAGVIELLDLSLHEYLR
                     ANISAKGYNPTDFVCFSYGGAGPVHTYGYTEGLGFKDVVVPAWAAGFSAFGCACADFE
                     YRYDKSVDIALPQGGAAAEQEEACRVIQTAWDELAAKVIEEFVINGFRAEDVTLLPGY
                     RMQYMGQLNDLEITSPIGTAASAADWEQITAAFEDTYERVYASSARSPELGFSVTGAI
                     MRGLVATQKPVLPEDPEAGPVPQPEAHLGQRPFYRHKRWVDADVWRMEALQPGNHIVG
                     PAVIESDSTTFVVPDGFETTLDKHRLFHLKECGRN"
     gene            4014..6332
                     /locus_tag="VIO83_11965"
     CDS             4014..6332
                     /locus_tag="VIO83_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY8332027.1"
                     /translation="MNMMAKNAPGSLLGNGKTLQAFRGEVLKRTQESGFYNGLSSLPF
                     RESDPIGYEKLFSKIRGGLVHARETAKKIAASPIVEQEGELCFTLYNAVGDCVLTSTG
                     IIIHVGTMGAAIKYMIENDWESNPGIAPGDMFTNNDCSIGNVHPCDIATIVPIFWAGK
                     LIGWVGGVTHVIDTGAVTPGSMSTGQVQRFGDGYQVTCRKTGVNDQPLRDWLHESQRS
                     VRTPKYWILDERTRIAGCHMIRSMVEEVIASVGLEAYEKFAFEVIEEGRRGLQSRIKA
                     MTLPGTYRKVAFVDVPFKHEDVQTSSAFAKVDSIMHSPVEITIRGDASWRLDFEGSSR
                     WGWHTFNAHQVAFTSGIWVMMTQTLVPTQRINDGASFGTEFHLPKGAWCNPDDRRTGH
                     AYAWHFLVSGWSALWRGLGQAYYSRGYLEEVNSGNANTSNWLQGGGINQDGEVHAVNS
                     FEASSCGTGAMAFRDGLNHAAAIWNPEGDMGDIEVWEMAEPLLYLGRNVKCNSGGYGK
                     YRGGCGFETLRMVWNAQDWTMFFMGNGYMNSDWGLMGGYPAATGYRFEAHQTGLEQRI
                     EQGLSLPLGGDRDPAAPAYEQHIAASARVKRDKQCMTTEDCYENHDLYLNYLRGGPGF
                     GDPLERELAAIADDLNQNFVLPAYAEKVYGAVLAQDAKGHWTVDAAATEVRRREIRAE
                     RLQRSQPTREWMREERERIVTKHASAPVQQMFASSFSLSEKFLAEFKAFWDLPANWTL
                     TEDELDQPTYGSKYRMDLSLLPDVHTVVQVEE"
     gene            6344..6850
                     /locus_tag="VIO83_11970"
     CDS             6344..6850
                     /locus_tag="VIO83_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetone carboxylase subunit gamma"
                     /protein_id="HEY8332028.1"
                     /translation="MSTYTKEQVKNLVDGRLDWDTTTRMLTMPKDRERFALYLNVLQE
                     RVAWPDRIVLPLGPALYIVQSATSKHWVTKCRCGHEFCDYRQNWKLHANIHVRDTPES
                     MQEVYPALLAPDTQWQVYREYYCPQCATQLDVEAPTPWYPVIHDFEPDIDTFYRDWLE
                     LPLPERAD"
     gene            6997..8961
                     /locus_tag="VIO83_11975"
     CDS             6997..8961
                     /locus_tag="VIO83_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEY8332029.1"
                     /translation="MTFPMSRGVFSCPEDAPRISAAWQAVLRGAAPPRDLLRHPVDDS
                     WRRCLHGRVDPGLQHAPEPLTDAQLFAARQRHRRLLDSSAPALAMAEELMAQSGAMLI
                     LADGEGMILEARGDAATLGRAEHIHLMPGSPWGEQVCGTNAIGTALLLGHPVQIHAAE
                     HFCEGIQRWTCSATVIHDPRDGQILGVIDVSGESRTFSRHTLALAETTAQRIESALAK
                     QEMASRFRLLEASIEHLAADRGEGVMLFDPHGFLLRSNARLPAALAAQGIVLEPGQSC
                     RLAGLALGADGRPAGPLPPGLSAAWLQPIWAGGEVLGTLVRIPLPARTGRPLVLPSPG
                     AEDEVGFARLVGDSPALTAALHKARQLATARVPILLQGETGVGKEAFAQGIHYSGVGR
                     DGPFVAINCGSLSRDLLASELFGYAEGAFTGARRGGMKGKIEAAQGGTLFLDEIGEMP
                     LELQSHLLRVLEEGELFRLGESEPRKVDFRLVAATHRDLRQEVAKQRFREDLFYRVAV
                     TQIRIPPLRERKEDIALLARHILQRQAGRHGRPLPELAPEVVAALAGHDWPGNVRELR
                     NVLEGMLLLGGPRLTGEDLPDELRPLGGADAAVEGLLDENERQVICQAIDRSRGNLTQ
                     AAKALGIAKSTLYLRLRKYGLSRGSGATAD"
     gene            9044..>9181
                     /locus_tag="VIO83_11980"
     CDS             9044..>9181
                     /locus_tag="VIO83_11980"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY8332030.1"
                     /translation="MNKLYPNAHAALDGLVADGMTLAVGGFGLCGIPEALIAALRDSG
                     RK"
BASE COUNT         1533 a         3239 c         3059 g         1350 t
ORIGIN      
        1 gcatgcactg cgccgacccc ggctgcctca aggcctgtcc gagcccgggg gcgatcgtca
       61 agtacgccaa cggcatcgtc gacttcaatc aggacaagtg catcggctgc ggctactgca
      121 tcaccggctg cccgttcgac gtgccgcgca tctcgcagaa ggaccacaag gcctacaagt
      181 gcaccctgtg cagcgatcgc gtggcggtcg ggctggagcc ggcctgcgtg aagacctgcc
      241 cgaccggggc catcgtcttc ggcagcaagg cggacatgca ggagcatgcc gccgggcgga
      301 tcgccgacct caaggagcgc ggcttcgacc aggccgggct gtacgatccc gacggcgtcg
      361 gcggcaccca cgtgatgtac gtgctgcacc acgccgacca gccctcgctg tacgccggcc
      421 tgcccaacgc gccggcgatc agcctgctgg tcagcctgtg gaagggcatc accaagccgc
      481 tggcgctgct ggccatgggc gccaccgtgc tggccggctt cttccactac gtgcgcatcg
      541 gcccgaaccg ggtcgacgag gaggaggacc ccggcgagcc gacggtgcac caggtcgatc
      601 cgacagtgca tgtggtcgat ccgaacaagc cctgagtctt tccaccatgc cctcccccga
      661 tggggggagg gccagggaga ggggccgctc acggtctggc ctcttccggg agccaacgat
      721 gaacaacgag atccagcgct acaccccctc ggagcggtcc aaccactggg ccgtggccat
      781 cctcttcttc ctcgccggcc tgtccgggct ggcgctgttc catccggcgc tgttcgggct
      841 gtccggcctg ttcggcggcg gggtgtggac gcgcatcctg catccgttcc tcggcttggc
      901 gatgttcgcg ttgttcctga tcatggccat gcgttttgcc gggcataacc gcctcaacgc
      961 caacgaccgc cagtggctcg ggcagatggg cgacgtgctc aacaaccgcg aggacaagct
     1021 gccggaagtc ggccgctaca acgccgggca gaagctgctg ttctgggtgc tggtgctgtg
     1081 catgctggtg ctgctggcca gcggcatcgt catctggcgc cagtacttcg gccagctgtt
     1141 cggcgtcggg ctgatccgtc tcgccgccgt ggcccatgcg ctggccgcct tcgtactgat
     1201 caccagcatc atcgtgcaca tctacgcggc gatctgggtc aagggttcgg tgggcgccat
     1261 gctcaccggc aaggtcagcc gcgcctgggc gcgcaagcac catccgggct ggttcaagga
     1321 cgtggagcgc cagcgcaaat agccgaaaag atccggagga tggcgtaacg gccagggcgg
     1381 actcggcgcg aagcgacagt ccgccgcgta ccactgcccg caacgggcaa gccctgcgaa
     1441 tccgcgggtc gctcccgcac cggcgtgagc gaagatcagc ggcgcctgtt caccaccggg
     1501 cgcggagcat cgccagcccg ggttgccacg caggcgcgtg gcaaccatcc agaaatttcc
     1561 tccctcccgc gccatcaccc cccgaccgcc cgctacccgg ccgcccgcgc aacgccggcg
     1621 agcgtccgaa tttcatacgg cgccggacgc gggtatccat gcatcgaacg tccgggtgcc
     1681 tccagaattt ccgtgcgctc agcgcaatat caaccatttc aattgcttgc gtgatgcatg
     1741 cggcgttggc acaggcgctg caaaaggact tgggcgggta gcgcggctgc ctgccagaca
     1801 agaacaaaaa cgggagaacc ctatggacat gcaacgctcg aaagtccagg tgatgggcat
     1861 cgatgccggc ggcacgatga ccgacacttt cttcgtccgc gaggacggca acttcgtggt
     1921 gggcaaggcg cagagcaatc cggccgacga gtccctggct atctacaact cgtccctcga
     1981 cgccttgtcc acctggggac agggcgtcga cgacgtctac cccgagctgg tgacctgcgt
     2041 gtattccggc accgccatgc tcaaccgggt ggtgcagcgc aagggcctgg atgtcggcct
     2101 gatcgtcaac cggggcttcg agcagaccca ctccatgggc cgcgccatcc agagctatct
     2161 gggctatgcc ctggaggagc gcctgcacct caacacccac cgctacgacg aaccactggt
     2221 gccctattcg cgcacccgcg gggtcaccga gcgcaccgac gtgcagggca aggtggtcat
     2281 cccgctgcgc gaggaggagg tgcgccaggc cacccgcgag ctggtcgagg ccggcgccca
     2341 ggccatcgcc atcgtgctgc tgcagtcgca caagaacgcc agcagcgaga agcgcgcccg
     2401 cgatgtctgc cgcgaggagc tgcagcgcct gggcagcgag atcccggtgt ttgcctcggt
     2461 ggactactac ccgtcgcgca aggaaagcca ccgcaccaac accaccatcc tcgaagccta
     2521 cgccgccgag ccctcgcggc agaccctggc gaaggtcagc gagcgcttca agaagcatgg
     2581 cgccaagttc gacctgcggg tgatggccac ccacggcggc accatctcct ggaaggccaa
     2641 ggagctggcg cggaccatcg tctccgggcc gatcggcggg gtgatcggtt cccggctgct
     2701 cggcgaggcg ctgggctacg agaacatcgc ctgctcggac atcggcggta cgtccttcga
     2761 catggcgctg atcaccaagg gcaacttcgc catcgcctcc gacccggaca tggcccgcct
     2821 ggtgctgtcg ctgccgctgg tggcgatgga ctcggtgggc gcgggggcgg gcagcttcgt
     2881 gcgcctcgac ccctacagcg gctcgatcaa gctgggcccg gacagcgccg gctaccgggt
     2941 cggcacctgc tggccggaaa gcggcctgga caccgtctcg gtgaccgact gccacgtggt
     3001 gctcggctat ctcaacccgg acaacttcct cggcggcgcc atcaagctcg acgtggagcg
     3061 cgcccgccgc cacatccagg agcagatcgc cggccccctc ggcctgtccg tcgaggacgc
     3121 cgcggccggg gtcatcgagc tgctcgacct gtccctgcac gaatacctgc gcgccaacat
     3181 cagcgccaag ggctacaacc ccaccgactt cgtgtgcttc tcctacggcg gcgccggccc
     3241 ggtgcacacc tacggctaca ccgaggggct cggcttcaag gacgtggtgg tgccggcctg
     3301 ggcggcgggg ttctcggcct tcggctgcgc ctgcgccgac ttcgagtacc gctacgacaa
     3361 gagcgtcgac atcgccctgc cgcagggcgg cgcggcggcc gagcaggagg aggcctgccg
     3421 ggtgatccag acggcctggg acgaactggc cgccaaggtc atcgaggagt tcgtcatcaa
     3481 cggcttcagg gccgaggacg tcaccctgct gccgggctac cgcatgcagt acatgggcca
     3541 gctcaacgac ctggagatca cctcaccgat cggcaccgcg gcctcggccg ccgactggga
     3601 gcagatcacc gcggccttcg aggataccta cgagcgggtc tacgccagct ccgcgcgctc
     3661 gccggagctc ggcttctcgg tcaccggggc gatcatgcgc ggcctggtcg ccacccagaa
     3721 gccggtgctg cccgaggacc cggaggccgg gccggtgccg cagcccgagg cgcatctcgg
     3781 ccagcggccg ttctaccggc acaagcgctg ggtcgacgcc gacgtctggc gcatggaggc
     3841 cctgcagccg ggcaaccaca tcgtcggtcc ggcggtgatc gagtccgatt cgaccacctt
     3901 cgtggtgccg gacggcttcg agaccaccct cgacaagcat cgcctgttcc atctcaagga
     3961 atgcggccgc aactgaatcg gaccccacaa gaacaacaag cgagacgatc accatgaaca
     4021 tgatggccaa gaatgcgccc ggctccctgc tgggcaatgg caagaccctg caggccttcc
     4081 gcggcgaggt gctcaagcgc acccaggaga gcggcttcta caacgggctg agcagcctgc
     4141 cgttccgcga gagcgacccg atcggctacg agaagctgtt ctcgaagatc cgcggcggcc
     4201 tggtgcacgc ccgggaaacg gcgaagaaga tcgccgccag ccccatcgtc gagcaggagg
     4261 gcgaactttg cttcaccctg tacaacgcgg tgggcgactg cgtcctcact tccaccggga
     4321 tcatcatcca cgtcggcacc atgggcgcgg cgatcaagta catgatcgag aacgactggg
     4381 agagcaatcc cggcatcgct cccggcgaca tgttcaccaa caacgactgc tcgatcggca
     4441 acgtgcaccc ctgcgacatc gccaccatcg tgccgatctt ctgggccggc aagctgatcg
     4501 gctgggtcgg cggggtgacc cacgtgatcg acaccggggc ggtgaccccg ggctcgatgt
     4561 ccaccggcca ggtgcagcgc ttcggcgacg gctaccaggt cacctgccgc aagaccgggg
     4621 tcaacgacca gccgctgcgc gactggctgc acgagtcgca gcgctcggtg cgcacgccca
     4681 agtactggat tctcgacgag cgcacccgca tcgccggctg ccacatgatc cgcagcatgg
     4741 tcgaggaggt gatcgcctcg gtggggctgg aggcctacga gaagttcgcc ttcgaggtca
     4801 tcgaggaggg ccgccgcggc ctgcagtcgc ggatcaaggc gatgaccctg cccggcacct
     4861 accgcaaggt ggccttcgtc gacgtgccgt tcaagcacga ggacgtgcag acctcctcgg
     4921 ccttcgccaa ggtcgacagc atcatgcact cgccggtgga gatcaccatc cgcggcgacg
     4981 ccagctggcg cctcgacttc gagggttcca gccgctgggg ctggcacacc ttcaacgccc
     5041 accaggtggc cttcaccagc ggcatctggg tgatgatgac ccagaccctg gtgcccaccc
     5101 agcgcatcaa cgacggcgcc agcttcggca ccgagttcca cctgcccaag ggcgcctggt
     5161 gcaacccgga cgaccgccgt accggccacg cctatgcctg gcacttcctg gtctccggct
     5221 ggagcgcgct gtggcgcggc ctcggccagg cctactacag ccgcggctac ctggaggagg
     5281 tcaactccgg caacgccaac acctccaact ggctgcaggg cggcggcatc aaccaggatg
     5341 gcgaagtgca cgcggtgaac agcttcgaag cctcgtcctg cggcaccggc gccatggcgt
     5401 tccgcgacgg cctcaaccat gccgcggcga tctggaaccc cgagggcgac atgggcgaca
     5461 tcgaggtctg ggagatggcc gagccgctgc tgtacctggg gcgcaacgtc aagtgcaact
     5521 ccggcggcta cggcaagtac cgcggcggct gcggcttcga gaccctgcgc atggtgtgga
     5581 atgcccagga ctggacgatg ttcttcatgg gcaacggcta catgaactcc gactgggggc
     5641 tgatgggcgg ctatccggcg gccaccggct accgcttcga ggcgcaccag accggcctcg
     5701 agcagcgcat cgagcagggc ctgtcgctgc cgctgggcgg cgaccgcgac cccgccgcgc
     5761 cggcctacga gcagcacatc gcggccagcg ccagggtcaa gcgcgacaag cagtgcatga
     5821 ccaccgagga ctgctacgag aaccacgacc tctacctcaa ctacctgcgc ggcggcccgg
     5881 gcttcggcga tccgctggag cgcgaactgg cggccatcgc cgacgacctg aaccagaact
     5941 tcgtcctgcc ggcctacgcg gagaaggtct acggcgcggt gctcgcccag gatgccaagg
     6001 gccactggac ggtggacgcg gcggctaccg aggtgcgccg ccgggagatc cgcgccgagc
     6061 gcctgcagcg ctcgcagccg acccgcgagt ggatgcgcga ggagcgcgag cggatcgtca
     6121 ccaagcacgc ctcggcgccg gtgcagcaga tgttcgccag cagtttctcc ctgtcggaga
     6181 agttcctcgc cgaattcaag gcgttctggg atctgccggc caactggacg ctgaccgagg
     6241 acgaactcga ccagccgacc tatggctcca agtaccgcat ggacctgtcc ttgctgccgg
     6301 acgtccatac cgtggttcaa gtggaagaat aaggagagtg agcatgagca cctataccaa
     6361 ggaacaagtg aagaacctgg tggacggccg tctggactgg gacaccacca cccgcatgct
     6421 gaccatgccc aaggaccgcg aacgcttcgc gctgtacctg aacgtgctgc aggagcgcgt
     6481 cgcctggccg gaccgcatcg tcctgccgct ggggccggcg ctgtacatcg tgcagtcggc
     6541 gaccagcaag cactgggtga ccaagtgccg ctgcggccat gagttctgcg actaccggca
     6601 gaactggaag ctgcacgcca acatccacgt gcgcgacacc ccggagagca tgcaggaggt
     6661 ctatccggcg ctgctggcgc cggacaccca gtggcaggtg tatcgcgagt actactgccc
     6721 gcagtgcgcc acccagctgg acgtcgaggc gccgacgccc tggtatccgg tgatccatga
     6781 cttcgagccg gacatcgaca ccttctatcg cgactggctg gagctgccgc tgcccgagcg
     6841 cgccgactga gggtgcgcca ccgccccgcg tccggcgggg cggtggcctt ggcgcgaagc
     6901 actggtctag actgcttgcc agccggctgc cagggagcgg gagggctgtc cgctaccctg
     6961 gagcgggcac aacaataaga acggaggtga cagccgatga ctttcccgat gtcccgcggg
     7021 gtgttttcct gcccggaaga cgccccgcga atcagcgcgg catggcaggc cgtgctgcgc
     7081 ggtgcggcgc cgccgcgtga cctgctccgc catccggtcg acgattcctg gcgacgctgc
     7141 ctgcacgggc gggtcgatcc gggtctgcag cacgcccccg agccgctgac cgacgcccag
     7201 ttgttcgccg cccgccagcg ccaccggcgc ctgctggaca gcagcgcgcc ggcgctggcc
     7261 atggccgagg agctgatggc gcagagcggc gccatgctga tcctcgccga cggcgagggg
     7321 atgatcctcg aggcgcgcgg cgacgcggcg accctcggcc gcgccgaaca catccacctg
     7381 atgcctggca gtccgtgggg cgagcaggtt tgcggcacca acgccatcgg caccgccctg
     7441 ctgctcggcc acccggtgca gatccatgcg gccgagcact tctgcgaggg catccagcgc
     7501 tggacctgct cggcgacggt gatccacgat ccgcgcgacg gccagatcct cggtgtcatc
     7561 gacgtttccg gcgaatcgcg gaccttcagc cggcataccc tggcgctggc cgagaccacc
     7621 gcccagcgca tcgaatcggc gctggccaag caggagatgg ccagccgctt tcgcctgctc
     7681 gaagccagca tcgagcacct ggccgccgat cgcggcgagg gggtgatgct gttcgatccc
     7741 cacggtttcc tgctgcgcag caatgcccgc ctgccggcgg cgcttgccgc ccagggcatc
     7801 gtcctcgaac ccggccagtc ttgccgcctg gccggccttg ccctgggggc cgacggtcgt
     7861 cctgccggcc cgctgccgcc cgggttgagc gccgcctggc tgcagccgat ctgggcgggc
     7921 ggcgaagtcc tcggcaccct ggtacgcatt ccgttgcccg cccggacggg gcggccgctg
     7981 gtcctgccgt cacccggcgc ggaggacgag gtcggcttcg cccgcctggt gggcgacagc
     8041 ccggcgctca ccgcagcgct gcacaaggcc cggcagctgg ccacggcgcg ggtgccgatc
     8101 ctgctgcagg gcgagaccgg cgtgggcaag gaggccttcg cccagggtat ccactactcg
     8161 ggcgtcggcc gcgacggtcc cttcgtcgcc atcaactgcg gcagcctgtc ccgcgacctg
     8221 ctggccagcg agctgttcgg ctatgccgag ggtgccttca ccggtgcccg gcgcggcggc
     8281 atgaagggca agatcgaggc ggcccagggc ggcaccctgt tcctcgacga gatcggcgaa
     8341 atgcccctgg aactgcaaag ccacctgctg cgcgtgctgg aggagggcga gctgttccgc
     8401 ctcggcgaga gcgagccgcg caaggtcgac ttccgcctgg tcgccgccac ccaccgcgac
     8461 ctgcgccagg aggtggccaa gcagcgtttt cgcgaggacc tgttctaccg cgtggcggtc
     8521 acccagatcc gcattccgcc gctccgcgag cgcaaggaag acatcgcgct gctcgcccgc
     8581 cacatcctgc agcgtcaggc cgggcgccat ggccggccgc tgccggaact ggctcccgag
     8641 gtcgtggccg ctctggccgg ccatgactgg cccggcaacg tgcgcgagct gcgcaatgtc
     8701 ctcgagggca tgctgctgct gggcggtccg cggctgaccg gcgaggacct gccggacgag
     8761 ttgcgcccgc tcggcggggc cgacgcggcg gtcgagggcc tgctggatga aaacgagcgc
     8821 caggtcatct gccaggccat cgaccgcagc cggggcaacc tgacccaggc ggccaaggcc
     8881 ctgggcatcg ccaagagcac cctgtatctg cggctgcgca agtacggcct gtcgcgtggc
     8941 tccggcgcga cggccgatta gccggcgctg cgcggctgct gagccatggt gcgctgcgcg
     9001 caccgacacc gacctccaga cagaacaaca agagaggcag cccatgaaca agctctaccc
     9061 caacgcccac gccgccctcg acgggctggt ggcggacggc atgaccctcg ccgtcggcgg
     9121 cttcggcctg tgcggcatcc ccgaggcgct gatcgccgcg ctgcgcgaca gcggccgcaa
     9181 g
//
LOCUS       DATCUN010000319         9266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_166964, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000319 DATCUN010000000
VERSION     DATCUN010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9266
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166964"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VIO83_11985"
     CDS             <1..209
                     /locus_tag="VIO83_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016704520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY8332031.1"
                     /translation="SVWCPRLGWVDFDPTNNLIPALEHITLAWGRDFGDVSPLRGVIL
                     GGGSHDPEVSVTVMPLAEALARGL"
     gene            341..1192
                     /locus_tag="VIO83_11990"
     CDS             341..1192
                     /locus_tag="VIO83_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY8332032.1"
                     /translation="MDESNLISAGTEKVSQLWQVVADYGVAFGVKILAAIAFWVVGRW
                     LIGFAVGLVQKTLGSQQVDPTVLRYLGSFITVTLNILLVIGILGYFGVQTTTFAALIA
                     AVGLAIGMAWSGLLANLAAGGFIIVLRPFKVGDFISAGGVTGTVTEIGLFATAITTPD
                     NVLTLVGNNKIFGDNILNYTANPFRRVELKAQLSGAADWKAAAALLKERIAALDNVLA
                     EPAVEVEILDFNLVGPVLAVRPFCHNDHYWQVYFDTNRVIKDALGEAGFPAPMPAQMV
                     IVQQAGG"
     gene            complement(1303..3195)
                     /locus_tag="VIO83_11995"
     CDS             complement(1303..3195)
                     /locus_tag="VIO83_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8332033.1"
                     /translation="MAISPIPVDQRVIDIDSRRFASIEKALVELITNADDSYARLEKA
                     GSPVTGQIRIHYERHQTGAVLMIADQAEGMSFEQARFILTYGGAHSPLARGEGGGRGY
                     FGRGLKQAIYGLGYGWIETIQAGRFSRIELFRSENGGYLYDDGDGDRPARDKDYARLG
                     ITENGTKVTIVIENALTNISHYRSVVQSVANNVYLRDVLSRRTVELVHMQGGRELERS
                     GIVHYQEPPATLLLGPEQAGHFVHEQQSYPFTLTLKRALDVELATRGDERTNGLVVLS
                     GTAVLDCQLFEYENQVGTEYLFGTVHCPALIEKLGQGVAIISDEREGLNHKEPFVEAF
                     AKAVSRMIAPSVLAEREKLKHLQHASASSRTSHMIEHLLERMNRAAIQDLGISLPPAA
                     ADASAADAAQRPAALRFTTPFYYRQPGHPFHVTLLLDPAQLASGEVLHVEYVLPDTLH
                     IDPAPTEIAVGELHGSERIEWTLVGDGAGNRGEIAVRAGTHWAWCEVVVAEHASKHSH
                     GTAGHAIRHRTPHDHGELMFTGYEFRNLGNELDRAVYSPAERKILINTAAPTVQLYVD
                     GRGHFRDSARLLLAELFMDVISDELARFAVEKSGQPGNVEAFHAAKLDIIRRYGSEIH
                     LSFLTA"
     gene            3510..3986
                     /locus_tag="VIO83_12000"
     CDS             3510..3986
                     /locus_tag="VIO83_12000"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEY8332034.1"
                     /translation="MKPLLLALLAVLPASAALAADPEVPYPTGYRHWHHVKSMVIEQG
                     HPLFEAFGGIHHIYANASALEGYKAGSFPDGAVIVFDRLEAKRADSAVTEGARKDVAV
                     MHKDAKKYAATGGWGFEAFGGGDPTRPVVGAGAATACYACHAPEKAHDYVFSRPRD"
     gene            complement(4036..4725)
                     /locus_tag="VIO83_12005"
     CDS             complement(4036..4725)
                     /locus_tag="VIO83_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrdJb"
                     /protein_id="HEY8332035.1"
                     /translation="MTVKIGQRIKSFKVVDASQRPAEASAQQQAQPPVQLDETLQRPE
                     MLVGVTYKIKSPLFEHALYVTINDILLNAGTPFEQRRPFEIFINSKNMDHFQWIVALT
                     RIMSAVLRKGGDCTFLVEELKAVFDPRGGYLKKGGVYMPSIVAEIGAVLERHLIAIGM
                     LQGQQPDEAQQRYLAERRAAFEASQGAAALEPGEGFPPGAQLCGHCHTQAVVQLEGCA
                     TCLNCGHSKCG"
     gene            complement(4852..6993)
                     /locus_tag="VIO83_12010"
     CDS             complement(4852..6993)
                     /locus_tag="VIO83_12010"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEY8332036.1"
                     /translation="MAKRGKSVLPAAGNIPLQAASADIWAQKYRLVEPDGTPVDADID
                     ATWRRVARALADVETPELREHWYEQFLWALRNGAIPAGRIVANAGAQERRPATSTINC
                     TVSGTIGDSMDDILTKLHEGGLTLKAGCGIGYEFSTLRPRGALVSGAGARTSGPLSFM
                     DIFDKMCFTVSSAGGRRGAQMATFDVAHPDVREFIRAKREDGRLRQFNLSLLISDDFM
                     QAVESDADWPLLFPVHPREVAELDLDDPQQVLWRDWPLHEGYAVRDDGTVACKVYGKL
                     RARHLWDMLMASTYDFAEPGFILIDRVNQLNNNWWCEEIRASNPCGEQPLPPYGACLL
                     GSVNLTVFVAAPFSIKARFDWQRFRQVVRVFTRLLDNVVELNNLPLEAQRREILAKRR
                     HGMGFLGLGSALAMLRLRYGSAEACRFTEDVAREMAVVGWQMALELAQEKGPAPLLME
                     EFAVTAKMLRQRPELVADGFKLGDKVPGRVLHARYSRYLQRIAEVAPELVAALAEHGA
                     RFTHHSSIAPTGTISLSLANNASNGIEPSFAHRYVRNLVRPGRKAKEQVEVLSYELLA
                     YRALVDAQADPDSIDPAHRLPEWFVSADDIGPTQHVDIQAAAQKWVDSSISKTANVPT
                     DYPFETFKDIYRYAWRQGLKGCTTFRFNPAAFQGVLVREADLANTLYRFELEDGSVVE
                     LKGNELVEYDGEVNSAANLFDALKEGYYGKY"
     gene            7271..8932
                     /gene="mdcA"
                     /locus_tag="VIO83_12015"
     CDS             7271..8932
                     /gene="mdcA"
                     /locus_tag="VIO83_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEY8332037.1"
                     /translation="MTTTISPPPQWSRRRSEKQRRLAQVRQLADGVVLPTDKIVAALE
                     ALLAPGDRVVLEGNNQKQADFLSRSLAKVDPGRLHDLHMIMPSVSRSEHLDLFERGIA
                     RKLDFSFAGPQSLRIGQLLEDGLLEVGAIHTYIELYSRLLVDLIPNVALVAGFIADRE
                     GNIYTGPSTEDTPALVEPTAFSDGIVIVQVNEIVDDPRDLPRVDIPASWVDFVVVADK
                     PFYIEPLFTRDPRHIKPVHVLMAMMAIRGIYEKHKVQSLNHGIGFNTAAIELILPTYG
                     ERLGLKGKICRNWTLNPHPTLIPAIETGWVESVHCFGTELGMENYVAARPDVFFTGRD
                     GSLRSNRMMCQLAGQYAVDLFIGATLQVDGDGHSSTVTRGRLAGFGGAPNMGHDPRGR
                     RHATPAWLDMTEPVTMLERGKKLVVQMVETYQEGGKPTFVEQLDAVEVARKVGMPLAP
                     VMIYGDDVTHLLTEEGIAYLYKARSLEERQAMIAAVAGVTSIGLRHDPKDTERMRREG
                     LIALPEDLGIRRTDASRELLAAKSIAELVDWSGGLYNPPAKFRSW"
     gene            8932..>9266
                     /locus_tag="VIO83_12020"
     CDS             8932..>9266
                     /locus_tag="VIO83_12020"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HEY8332038.1"
                     /translation="MNAQIAVRPRLSLAEQLADLAVETLIDEVELSPKPALVDRRGSG
                     AHSDLSLELMHASAWSLWATFHDMAAAGQAAGQVDVALRETLGRLGRDGEAQMLAVTG
                     GVNTHRGAI"
BASE COUNT         1487 a         3085 c         3092 g         1602 t
ORIGIN      
        1 tttcggtgtg gtgcccgcgc ctgggctggg tcgacttcga ccccaccaac aacctgattc
       61 cggccctgga gcacatcacc ctggcctggg gccgcgactt cggcgacgtt tcgccgctgc
      121 gcggggtgat cctcggcggc ggcagccacg atcccgaggt cagcgtgacg gtgatgccgc
      181 tggcggaggc gttggcgcgg gggctgtgag gaggcggcct ggctgtcgcg cggcggttgc
      241 cggcgcaggg tgcaccgccc cggcgactcg gcgggagtag cggtttttcg cgcggctgct
      301 agcgtttcat gggtcagccc ctttgcgcag gtgatccgcc atggacgaaa gtaatctgat
      361 cagtgccggt accgaaaagg tcagtcaact ctggcaggtg gtcgccgact acggcgtggc
      421 tttcggcgtc aagatcctcg ccgccatcgc cttctgggtg gtcggccgct ggctgatcgg
      481 cttcgccgtc ggcctggtgc agaagaccct gggcagccag caggtcgacc cgacggtgct
      541 gcgctacctt ggctccttca tcaccgtcac cctcaacatc ctgctggtga tcggcatcct
      601 cggctacttc ggcgtgcaga ccaccacctt cgccgcgctg atcgccgcgg tcggcctggc
      661 catcggcatg gcctggtcgg ggttgctggc caacctggcg gccggcggct tcatcatcgt
      721 tctgcgccca ttcaaggtcg gcgacttcat cagcgccggc ggggtgaccg gcacggtcac
      781 cgagatcggc ctgttcgcca ccgcgatcac cacgccggac aacgtgctga ccctggtcgg
      841 caacaacaag atcttcggcg acaacatcct caactacacg gccaacccgt tccgccgcgt
      901 cgagctcaag gcccagctgt ccggtgccgc tgactggaag gccgcggccg ccctgctcaa
      961 ggagcgcatc gccgccctcg acaacgtgct ggccgagccg gcggtggaag tggaaattct
     1021 cgacttcaac ctggtcggcc cggtgctggc cgtgcgcccc ttctgtcaca acgaccacta
     1081 ctggcaggtc tacttcgaca ccaaccgggt gatcaaggat gctctcggcg aagccggctt
     1141 cccggcaccg atgccggcgc agatggtgat cgtgcagcag gccggcgggt agcacggcct
     1201 gtcccatcgg cgcgggacga catttgtctt tcgtcggagc cagcttgctg gcgatgtgct
     1261 tcgcgccaca aagcatcgcc agccagctgg ctcctacggc cctcaggcgg tgaggaacga
     1321 cagatggatc tcgctgccgt agcgacgaat gatgtccagc ttggcggcgt gaaaggcctc
     1381 gacattgcct ggctggcctg acttctcgac cgcgaagcgg gccagctcat cggaaatcac
     1441 atccatgaac agctcggcca gcagcagccg ggcggagtcg cggaaatgcc cgcggccatc
     1501 cacgtacagc tgcacggtcg gtgccgcggt gttgatcagg atcttccgct ccgccgggct
     1561 gtagaccgcg cggtccagct cgttgccaag gttgcggaac tcgtagccgg tgaacatcag
     1621 ctcgccatga tcgtggggtg tccgatgccg gatggcatgg ccggctgtgc catggctgtg
     1681 ttttgaagca tgctcggcca ccaccacctc gcaccaggcc caatgggttc ccgcccgcac
     1741 cgcgatctcg ccgcggttgc cggcgccatc ccccaccagc gtccattcga tccgctcact
     1801 gccgtgcaat tcgccaaccg caatctccgt cggcgccggg tcgatgtgca gggtgtcggg
     1861 cagcacatat tcgacatgca gcacctcgcc actggccagc tgggcggggt cgagcagcag
     1921 ggtgacgtgg aacggatgcc cgggctggcg gtaatagaac ggcgtggtga agcgcagcgc
     1981 cgccgggcgt tgtgccgcat cagcggcgct cgcatccgct gcggcgggcg gcaggctgat
     2041 gcccagatcc tggatggccg cccgattcat gcgctcgagc aggtgctcga tcatatggct
     2101 ggtgcggctg gaggcgctgg catgctgcag atgcttgagc ttttcccgct cggccagcac
     2161 gctgggggcg atcatgcggc tgaccgcctt ggcaaatgcc tccacgaagg gctccttgtg
     2221 attcagtccc tcgcgctcgt cgctgatgat ggccacgccc tggccgagct tttcgatcaa
     2281 ggccgggcaa tgcaccgtgc cgaacaggta ttcggtgccc acctggtttt catattcgaa
     2341 cagttggcaa tccagtaccg ccgtgccgga caggacaacc aagccattgg tgcgctcatc
     2401 gcccctggtc gccagttcca catccagagc gcgcttgagg gtgagggtga acggatagct
     2461 ctgttgctca tgcacgaaat gcccggcctg ctctgggccc agcagcagcg tggcgggtgg
     2521 ctcctggtaa tgcacgatac cgctgcgctc gagttccctg ccgccttgca tatgcaccag
     2581 ttcaacggtg cgccggctca gcacatcgcg cagatagacg ttgttcgcca ccgactgaac
     2641 aacggatcga tagtgggaga tgttggtcag tgcgttttca atgacgatgg tgaccttggt
     2701 gccgttttcg gtaattccca ggcgggcata atccttgtcc cgggctggcc gatcgccgtc
     2761 gccgtcgtca tacaggtagc cgccattctc gctgcggaat aactcgatgc gggaaaatcg
     2821 tccggcctgg atggtctcga tccagccata acccaggcca tagatggcct gcttcaagcc
     2881 ccggccgaaa taaccgcgcc cgccgccctc gccccgcgcc aagggactat gcgccccgcc
     2941 ataggtcagg atgaagcggg cctgttcgaa ggacatcccc tcggcctgat cggcaatcat
     3001 cagcaccgcg ccggtctgat gccgctcata gtggatgcgg atctggccgg tcaccggcga
     3061 gccggccttt tccagccggg catagctgtc gtcagcgttg gtgatcaatt cgaccagggc
     3121 tttttcgatg gacgcaaagc gccgcgagtc gatatcgatc acccgttgat ccaccgggat
     3181 aggggaaatg gccatgcagt actccttgtt caactcattg accggcacgc cataagcgct
     3241 gccacgtcac ctgctttgac agcgatctgg ggcgatccta cgctgctcaa gcatcgactc
     3301 gatgcatgcg tgtgtccagg cgggtcacga gcaggtttgg gcttttttct gccgtgcgct
     3361 ggccggagcg gttgtggggg gagtcgagac ggtgctccag ggcgatttcc gccgctttcc
     3421 ggctgtctcg cctttgtccg ggtaatcacg ggtcggttcc aggcgctggc tgcagcgtgg
     3481 cccggccgac ccgccacgag gatcacacca tgaaaccgct actgcttgcc ctgttggccg
     3541 tgctgcccgc ttccgcggcg ctggccgcag accccgaggt tccctatccg accggctatc
     3601 gccactggca tcacgtcaag agcatggtga tcgagcaggg ccatccgctg ttcgaggcct
     3661 tcggtggcat ccaccacatc tatgccaacg cgtcggccct ggaaggctac aaggccggga
     3721 gctttccgga tggcgcggtg atcgtcttcg accggctgga agccaagcgt gccgacagcg
     3781 cggtgaccga gggtgcgcgc aaggacgtgg ccgtgatgca caaggatgcg aagaagtacg
     3841 ccgcgacggg cggctggggc ttcgaagcct tcggcggcgg tgatccgacc cggccggtgg
     3901 tcggtgccgg cgcggcgacc gcctgctatg cctgccatgc tccggagaag gcccatgact
     3961 acgtgttcag tcggccgcgc gactgaatca gcgccatcag gcagggcgca gtgggcactg
     4021 cctgaagagt aaggctcagc cgcacttcga atgcccgcag ttcaggcagg tggcgcagcc
     4081 ttccagctgc accaccgcct gggtgtggca gtgcccgcag agctgggcgc cgggcgggaa
     4141 gccttcgccc ggctccagcg ccgcggcgcc ctggctggct tcgaaggcag cgcggcgttc
     4201 ggccagatag cgctgctgcg cttcgtccgg ctgctggcct tgcagcatgc cgatggcgat
     4261 caggtggcgc tccagcacgg cgccgatctc ggcgacgatc gacggcatgt acacgccacc
     4321 ctttttcagg tagccgccgc gcgggtcgaa caccgccttg agctcctcga ccaggaaggt
     4381 gcagtcgccg cccttgcgca gcaccgcgga catgatgcgg gtgagggcga cgatccactg
     4441 gaagtggtcc atgttcttcg agttgatgaa gatctcgaag ggccgccgct gctcgaaggg
     4501 cgtgccggcg ttgagcagga tgtcgttgat ggtcacgtag agggcgtgct cgaacagcgg
     4561 cgacttgatc ttgtaggtga cgccgaccag catctccggg cgctgcaggg tttcgtcgag
     4621 ctgcaccggc ggctgtgcct gctgttgcgc ggaggcctcg gcggggcgct ggctcgcgtc
     4681 gaccaccttg aagctcttga tgcgctgccc gatcttcacg gtcatggtgt tctcacgatg
     4741 cgcaggttgt gcgggtaggg tgggtaacgg cgcagcctta cccaccgttg tgggcctgcg
     4801 gtggaaaacg ctgcgcggtt ttccacccta caaagacgtc cgcctccgcc ttcaatactt
     4861 gccgtaatag ccttccttga gcgcgtcgaa caggttggcg gcgctgttca cctcgccgtc
     4921 gtactcgacc agttcgttgc ccttgagctc gactacgctg ccgtcctcca gctcgaagcg
     4981 gtacagggtg ttcgccaggt cggcctcgcg caccagcacg ccttggaagg cggccgggtt
     5041 gaagcggaag gtggtgcagc ccttgaggcc ctggcgccag gcgtagcggt agatgtcctt
     5101 gaaagtctcg aacgggtagt cggtgggcac gttggcggtc ttggagatcg acgagtcgac
     5161 ccacttctgc gccgccgcct ggatgtcgac gtgctgggtc gggccgatgt cgtcggcgct
     5221 gacgaaccac tccggcagcc ggtgcgccgg atcaatgctg tccggatcgg cctgcgcatc
     5281 gaccagcgct cggtaggcca gcagctcgta gctcagcacc tcgacctgct ccttggcctt
     5341 gcgtccgggg cgcaccaggt tgcgcacgta gcggtgggcg aagctcggct cgatgccgtt
     5401 ggaagcgttg ttggccaggc tcaggctgat ggtgccggtc ggcgcaatcg agctgtggtg
     5461 ggtgaagcgc gcgccgtgct cggccagcgc cgcgaccagc tcgggagcga cctcggcgat
     5521 gcgctgcagg tagcgcgagt agcgcgcgtg cagcacgcgg cctggcacct tgtcgccaag
     5581 cttgaagcca tcagcgacca gttccgggcg ctggcggagc atcttcgccg tcaccgcgaa
     5641 ctcctccata agcagcggtg ccgggccttt ctcctgcgcc agttccagcg ccatctgcca
     5701 gccgaccacc gccatctcgc gggccacgtc ctcggtgaac cggcaagcct cggcgctgcc
     5761 gtagcgcagg cgcagcatcg ccagcgcgga accgaggccg agaaaaccca tgccgtggcg
     5821 gcgcttggcg aggatctcac ggcgctgcgc ctccagcggc aggttgttga gctccaccac
     5881 gttgtccagc aggcgggtga acacgcgcac cacctggcgg aagcgctgcc agtcgaagcg
     5941 cgccttgatc gagaacggcg ccgcgacgaa cacggtcagg ttgaccgagc cgagcaggca
     6001 ggcgccgtag ggcggaaggg gctgttcgcc gcaaggattg ctcgcgcgga tctcctcgca
     6061 ccaccagttg ttgttcagct ggttgacccg gtcgatgagg atgaagcccg gctcggcgaa
     6121 gtcgtaggtc gaggccatca gcatgtccca caggtggcgg gcgcgcagct tgccatagac
     6181 cttgcaggcc accgtgccgt cgtcgcgcac ggcgtagccc tcgtgcagcg gccagtcgcg
     6241 ccagagcacc tgctgcggat cgtccaggtc cagttcggcc acctcgcgcg ggtgcaccgg
     6301 gaacagcagc ggccagtcgg catcgctctc cacggcctgc atgaagtcgt cgctgatcag
     6361 caggctgagg ttgaactggc gcaggcgacc gtcctcgcgc ttggcgcgga tgaactcgcg
     6421 cacgtccggg tgggcgacgt cgaaggtggc catctgcgcg ccgcgccgcc cgccggcgga
     6481 gctgacggtg aagcacatct tgtcgaagat atccatgaac gacagcggcc cgctagtgcg
     6541 cgcgccggcg ccggacacca gcgcgccgcg cgggcgcagg gtggagaact cgtagccgat
     6601 accgcaaccg gccttgaggg tcagcccgcc ttcatggagc ttggtaagga tgtcgtccat
     6661 cgagtcgccg atggtgccgg agacggtgca gttgatggtc gaggtggccg gcctgcgctc
     6721 ctgcgcgccg gcgttggcga cgatccgccc ggccgggatg gcgccgttgc gcagcgccca
     6781 gaggaactgt tcgtaccagt gctcgcgcag ttcgggcgtc tcgacgtcgg ccagcgcgcg
     6841 ggcgacccgc cgccaggtgg cgtcgatgtc ggcgtcgact ggtgtgccat caggctcgac
     6901 cagccggtac ttctgcgccc agatgtccgc cgaggcggcc tgcagcggga tgttgccagc
     6961 cgccggcagg acggacttgc cgcgtttggc catgggcccg gatctcccat cggtcgaagg
     7021 atcttttcac cgtagccgca gctcagcgag cgcgcacggc ggtcagccgg ggggatgtcc
     7081 gtcggcggca tcggcgcttg cctaccgagg catgtcgccg atatccgggg gcgcggatgg
     7141 agccggttgt gaggtggcga tccattgaaa ccattaacct tggagtaatg aatgcacgtc
     7201 gagcttgatt gaacgccccc cgcccgaaac cctaccgtga ctccacgaca gcggaacccg
     7261 tggagtagcc atgacaacaa caatttcccc cccgccgcag tggtcgcgtc ggcgcagcga
     7321 gaagcagcgc cgcctcgccc aggtgcgcca gctggccgac ggcgtggtcc tgccgaccga
     7381 caagatcgtc gccgcgctgg aagcgctgct cgcccccggc gatcgcgtgg tgctggaagg
     7441 caacaaccag aagcaggcgg acttcctgtc ccgctcgctg gccaaggtcg atccgggccg
     7501 cctgcacgat ctgcacatga tcatgcccag cgtcagccgc agcgagcacc tggacctgtt
     7561 cgagcgcggc atcgcccgca agctggactt ctccttcgcc ggcccgcaga gcctgcgcat
     7621 cggccaactg ctggaggacg gcctgctgga agtcggtgcc atccacacct acatcgagct
     7681 gtactcgcgc ctgctggtcg acctgatccc caacgtcgcc ctggtcgccg gctttatcgc
     7741 cgaccgtgaa ggcaacatct ataccggccc gagcaccgag gacaccccgg cgctggtcga
     7801 gccgacggca ttcagcgacg gcatcgtcat cgttcaggtc aacgagatcg tcgacgatcc
     7861 gcgcgacctg ccgcgcgtgg acatcccggc ttcctgggtc gacttcgtgg tggtggccga
     7921 caagccgttc tacatcgagc cgctgttcac ccgcgacccg cgccacatca agccggtgca
     7981 cgtgctgatg gcgatgatgg cgatccgcgg catctacgag aagcacaagg tgcagtcgct
     8041 caaccacggc atcggcttca acaccgcggc catcgagctg atcctgccga cctacggcga
     8101 gcgcctcggc ctcaaaggca agatctgccg caactggacg ctcaacccgc acccgaccct
     8161 gatcccggcc atcgagaccg gctgggtgga gagcgtgcac tgcttcggca ccgagctggg
     8221 catggagaac tacgtggcgg cgcgcccgga cgtgttcttc accggccgcg acggatcctt
     8281 gcgctccaac cgcatgatgt gccagctggc cggccagtac gcggtcgacc tgtttatcgg
     8341 cgcaacgttg caagtggatg gcgacggcca ttcctccacc gttacccgcg gccgcctggc
     8401 cggcttcggc ggcgcgccga acatgggcca cgacccgcgc ggccgccgtc atgccacgcc
     8461 ggcgtggctg gacatgaccg agccggtgac gatgctcgag cgcggcaaga agctggtggt
     8521 gcagatggtc gagacctacc aggagggcgg caaacccacc ttcgtcgagc agctcgacgc
     8581 ggtggaggtg gcgcgcaagg tcggcatgcc gctggcgccg gtgatgatct acggcgacga
     8641 cgtcacccac ctgctcaccg aggagggcat cgcctacctg tacaaggccc gttcgctgga
     8701 ggaacgccag gcgatgatcg ccgcggtggc cggcgtcacc agcatcggcc tgcgccacga
     8761 cccgaaggac accgagcgca tgcgccgcga agggctgatc gccctgccgg aggatctggg
     8821 catccgccgc accgacgcca gccgtgaact gctcgccgcc aagagcatcg ccgagctggt
     8881 cgactggtcc ggcggcctgt acaacccgcc cgccaagttc aggagctggt gatgaacgca
     8941 cagattgccg ttcgcccgcg cctgagcctg gccgagcagc tcgccgatct cgccgtcgag
     9001 acgctgatcg acgaggtcga gctgtcaccc aagccggcgc tggtcgaccg ccgcggcagc
     9061 ggcgcccaca gcgacctcag cctcgagctg atgcacgcct cggcgtggtc gctgtgggcg
     9121 accttccacg atatggccgc cgccggccag gcggccgggc aggtcgatgt ggccctgcgc
     9181 gaaaccctcg gccgcctcgg ccgcgacggc gaggcgcaga tgctcgcagt caccggcggg
     9241 gtcaacaccc accgcggggc gatctg
//
LOCUS       DATCUN010000320         2523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_186095, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000320 DATCUN010000000
VERSION     DATCUN010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186095"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VIO83_12025"
     CDS             <1..261
                     /locus_tag="VIO83_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8332039.1"
                     /translation="ALRRPPPAEQLAAIPGVAGAHPQGEGRWLVSFEPGADPTDALVH
                     SAAIGDWGLYELTRPRLSLEDVFLELAGDEVPESAPAQEQAA"
     gene            258..1019
                     /locus_tag="VIO83_12030"
     CDS             258..1019
                     /locus_tag="VIO83_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8332040.1"
                     /translation="MRAVLAIAGRELRVLFASPSAWAMLAVVQAILGYLFLTQIDMFL
                     QLRSQLMAMPDPPGLTEIVAPSLFGSAGIVLLMIVPLITMRSFAEERRNQTLPLLLSA
                     PVSVSGIVFGKYLALMGFLTLVTALTAMMPVSLLLGARLDLGLLAASVLGLWLLLGSF
                     AALGLFMSTLTSHPMTAAVSTFGLLLLLWILDWASGSAGEGSGELLRQLSMLNHYQPL
                     LKGVFAIADVAYFALFIVLFLGLSIRRLDAERLAG"
     gene            1024..2367
                     /locus_tag="VIO83_12035"
     CDS             1024..2367
                     /locus_tag="VIO83_12035"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HEY8332041.1"
                     /translation="MTGSNSSRFTLRLQGAVALLLFLAVIGLLGYLAHTYDRRFDWTA
                     SGRNSLSDASRQVLEKLPEPVTATVFARPNPELRASLDDLLRRYAAASPKFTVSFVNP
                     DQAPARVKELGIRADGTVLLELAGRREKLEQLDEQALTSALMRLSRSGERRLIFLAGH
                     GERSPDGQANHDLSTFTTALREQGVLATRPDPASGKDFPTDATLVITAPAVDLTGDEV
                     GAIQRFLTAGGNLLWLADPGPLHGLEPIAAELGLRFVPGTLIDPVGQAIAGSAHFAIA
                     TADNYRFHAALAGFEFMTLFPLAAGLEHDPKEPWAATDLIELGASGWAETGPLTEKVS
                     FDEGQDRRGPFILAAAFTRKLGEREQRVVVVGDGDFLSNSYVGNGGNLNLGLNLVNWL
                     AADEGLVSIPPRTAPDTELSLSRDNSLLIGFGFLFGLPAAFAAAGLGVWLRRRGR"
BASE COUNT          341 a          891 c          865 g          426 t
ORIGIN      
        1 gccctgcgcc ggccgccccc ggcagagcag ctggcggcca tccccggcgt cgccggcgcg
       61 cacccgcagg gcgagggccg ctggctggtc agcttcgaac ccggcgccga tccgaccgac
      121 gccctggtac acagtgcagc catcggcgac tggggcttgt acgagctgac ccgcccgcgc
      181 ctgtcgctgg aagatgtgtt cctggagctg gccggggacg aggtgcccga gtccgccccg
      241 gcgcaggagc aggcggcgtg agggcggtgc tggccatcgc cgggcgcgag ctgcgcgtgc
      301 tgttcgcctc gccatcggcc tgggccatgc tggccgtggt gcaggccatc ctgggctatc
      361 tgttcctgac ccagatcgac atgttcctgc agctgcgctc gcagctgatg gccatgcccg
      421 acccgccggg cctgaccgag atcgtcgccc cgtcgctgtt cggcagtgcc ggcatcgtgc
      481 tgctgatgat cgtgccgctg atcaccatgc gcagcttcgc cgaggagcgg cgcaaccaga
      541 ccctgccgct gctgctgtcg gcaccggtgt ccgtgtccgg catcgtgttt ggcaagtatc
      601 tggcgctgat gggatttctg acgctggtga cggcgctcac ggccatgatg ccggtcagcc
      661 tgctgctggg cgccaggctc gatcttggcc tgctggcggc cagcgtgctg ggtctttggc
      721 tgctgctggg cagcttcgcc gcgctgggcc tgttcatgtc gacgctgacc tcgcatccga
      781 tgacggcggc ggtgagcacc ttcggcctgc ttctgctgct gtggatcctg gactgggcca
      841 gcggcagcgc cggcgagggc agcggtgagt tgctgcgcca gttgtccatg ctcaaccact
      901 accagccgct gctcaagggc gtgttcgcca ttgccgacgt ggcgtatttc gccttgttca
      961 tcgtgctgtt cctgggtctg agcatccggc ggctggatgc cgagcggctg gccggctgag
     1021 cacatgacgg gttccaactc cagccgcttc acgctgcgcc tgcaaggcgc cgtcgcgctg
     1081 ctgctgttcc tggccgtgat cggcctgctg ggctacctgg cgcacaccta cgaccgccgc
     1141 ttcgactgga ccgcaagcgg gcgcaactcg ctgtcggacg ccagccggca ggtgctggaa
     1201 aagctccccg agccggtcac cgccacggtg tttgcccgtc ccaaccctga actgcgcgcc
     1261 agcctcgacg acctgctgcg gcgctacgcg gccgcctcgc ccaaattcac ggtcagtttc
     1321 gtcaatcccg atcaggcgcc ggcgcgggtc aaggaactgg gcatccgcgc cgacggcacc
     1381 gtgctgctgg aactggccgg ccggcgcgag aagctcgaac agctcgacga gcaggcgctg
     1441 accagcgccc tcatgcgcct gtcgcgcagc ggcgagcgca ggctgatttt cctggccggc
     1501 cacggcgagc gcagcccgga cgggcaggcc aaccacgatc tgtccacctt caccacggcg
     1561 ctgcgcgagc agggcgtgct ggcgacgcgc ccggacccgg ccagtggcaa ggattttccg
     1621 accgatgcga cgctggtgat caccgccccg gcagtcgacc tgaccggcga cgaggtgggc
     1681 gccatccagc gtttcctgac cgccggcggc aacctgctgt ggctggccga tccggggcca
     1741 ctgcacggcc tggaaccgat cgcggccgag cttggcctgc gtttcgtgcc cggcacgctg
     1801 atcgacccgg tgggccaggc catcgccggc agcgcgcact tcgccatcgc cacggcggac
     1861 aactaccgct tccacgccgc gcttgcgggc ttcgagttca tgaccttgtt tcccctggcg
     1921 gccggcctcg agcacgatcc gaaggaaccg tgggcagcca ccgacctgat cgaactgggc
     1981 gcctccggct gggccgagac cggcccgctg accgaaaaag tgagcttcga cgagggccag
     2041 gaccggcgcg gcccgttcat cctggcagcg gctttcacac gcaagctggg cgagcgcgaa
     2101 cagcgcgtgg tggtggtcgg cgacggcgat ttcctgtcca acagctacgt cggcaatggc
     2161 ggcaacctga acctgggcct gaatctggtg aactggctgg cggccgacga gggtctggtc
     2221 agcatcccgc cgcgcacggc gccggacacg gagctgtcgc tatcgcgcga caacagtttg
     2281 ctgatcggct tcggcttcct gttcggcctg ccggcggcct tcgcggcagc cggcctgggt
     2341 gtctggctgc gccgccgggg acgctgatgc gcggtcggct atggctgaac ctgggtcttg
     2401 cactgctcgt cgtcgccctg gcggcggtgg cctggttcca gcccggcaag acgccgccgc
     2461 caccccaggt caagctcacg aacctcgacc cgcagacggt caagcgcatc gagttctacc
     2521 cgc
//
LOCUS       DATCUN010000321         7544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_71935, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000321 DATCUN010000000
VERSION     DATCUN010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71935"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VIO83_12040"
     CDS             <1..654
                     /locus_tag="VIO83_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019094270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8332042.1"
                     /translation="VAAAFAPWPSAKALAALLAGADIWYHARLSLQRVGVGLLLAFAF
                     GVPLGVLTGLSRSFSQATTPVFQFLRMISPLSWMPIAVMVLGVGDAPVYFLLAFAAVW
                     PILLSTAVGVAQLDANWLLLARSLSATRRETLFRVILPGITAQILAGVRLSIGIIWIV
                     LVPAEMLGVSAGLGYFILDTRDRLAYSELMAAIVLIGALGYLLDLLARWLHARWLHA"
     gene            complement(722..1663)
                     /gene="panE"
                     /locus_tag="VIO83_12045"
     CDS             complement(722..1663)
                     /gene="panE"
                     /locus_tag="VIO83_12045"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020288684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY8332043.1"
                     /translation="MRFLVVGAGALGGYFGGRLLQAGHDVTFLLRPARAAQLAASGLV
                     IHSPAGDLHLASPPTVLAHQLQPHYDVVIVGCKAYDLDETIASFAAAVGPETVVLPLL
                     NGLRHLDTLTARFSYRRVLGGLGLISATLDETGAVLHLSQDHTLAFGELDGERTPRVE
                     AIAAAFAQAAFVGRLSDSILQEMWEKWVHIATIASITTLMRASIGDIVGAGADGLALD
                     LLDECSRIAAHNGFAPRPEAIARTRATVTAPDSRLTASLLKDIERGAPTEGEHILGDF
                     LRRGRWSTADTILLPVAYAQVKSYERQRAGRAPLLHA"
     gene            1940..3100
                     /locus_tag="VIO83_12050"
     CDS             1940..3100
                     /locus_tag="VIO83_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8332044.1"
                     /translation="MLNRHTFLRIAALLLAAPMAALAAPASGEPLYFAVSGPLTGQNA
                     QYGAQWKAGFDLALDGINAEGGIHGRPLAYVFEDSQSDPRQAVGLAQKFVNDKRFLIE
                     LGDFSSPASMAASPIYQRAGLVQFGFTNSHPDFTKGGDYIWSPSISQADAQPILAELA
                     VKVRGLKRVAVLFLNTDWGRTSKDVFVKSAEALGAQVVIAEGYQPEEKDFRSTLVRVR
                     DAKPDGLVLISYYADGALIAGQVRSVGLTQPIVASSSVYSPKFLELGGAAVEGVLTNT
                     SFFPGEPRSEVQRFVQGFRAEYGKEPDAFNAYAYDAVQFAAAALRAAGPQADRKGVRD
                     VLYKIKDVPSVVFGKATFDPQTRRVIGVRSVHLVVRNGQWGVADSQGSVASR"
     gene            3118..4953
                     /locus_tag="VIO83_12055"
     CDS             3118..4953
                     /locus_tag="VIO83_12055"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8332045.1"
                     /translation="MTFSFTSFFDYTVNGLIIGNVYALLAVGLALIFGVSHLINFAHG
                     SVYTVGAYIGWASITYLHTPLPLTLLGVVLGSGALGMLIERVGLRPLQGAARIAPLLA
                     TIGISFVLDILVQLVFSPDPRALPTQLPDWRLQIGSGTIGALDLLIAGIGLASAGVLF
                     GFLRFTRLGWAVRATAQDRDAAQQMGVDVNRVNQTVFAIASALGGLSGVLVGMYYNNI
                     SPAMSFQATLKGVVATVVGGVGNVPGAIVGSLLLGLTESYGVALFGTPYRNLFAFVLL
                     LLILLWKPNGLFGRRRALPPEPLTGTFIAPSRPWPVPRWLLVALFAIAAVLPLVEPSP
                     YVLQTLTNSWLYAALALSLTLVAGTIGQISLGHAGLLAIGAYASSLLTLDLHLPVGTS
                     IIVAGLLTSVIGTALIFPAFRLRGHYVSIATLGIGEIVALTILNWESLTRGPIGVSGI
                     PPLSLFGQDASSNNAFYWASFGAMVLLALLQWRLLRSHLGRTFRAVREDEVAARAYGV
                     NPDRYKAMAFGFGGFAAGVSGAFTAHLYSYINHETFTSQISILALTMVILGGLGNISG
                     ALLGAVALVSLPELFRVTAEYRMLIYGIALLLLIRFRPQGLLGTV"
     gene            4967..5548
                     /locus_tag="VIO83_12060"
     CDS             4967..5548
                     /locus_tag="VIO83_12060"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY8332046.1"
                     /translation="MSHLNDYLAEKRAAVLARNAAIEAGTAQPAQLKATVRAEGRSSV
                     RRLRIRDHQVISDSPPDFAGYNLGPSSPELQLGVLGTCVTHIFLIQAAERQVPLESLE
                     VEVTGVIDPRGGKPGHEQTPIWPHQIGYTVHIDSPASRADIDALFEAVERNCPILNLL
                     RNPQTIRAEVRLVNSRAASVPTDAKAADAVATA"
     gene            5529..6371
                     /locus_tag="VIO83_12065"
     CDS             5529..6371
                     /locus_tag="VIO83_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8332047.1"
                     /translation="MPSPLLEVAALTRRFGGLTAVDGVDLQVREGELVSVIGPNGAGK
                     TTLFNLVTGLDQPDGGRVLFGGTDITGLPAQELARRGLARTFQHGRVFANLSVLDNVL
                     VGAHTRLRAAKPAMRGVPGLAAIAELALGLLQPPSVLREARHLREHALEIIALFGDRL
                     TPRIHQPAHSLSYANRRRLEIARALALQPQLLLLDEPTAGMNESETVEMLQIIRGLKQ
                     RGETILLIEHKLDLVMQLSDRVVVLDDGRKIAEGLPEQVRHDPAVIEAYLGHRHIGEQ
                     VVAA"
     gene            6368..7213
                     /locus_tag="VIO83_12070"
     CDS             6368..7213
                     /locus_tag="VIO83_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8332048.1"
                     /translation="MIATVARSGLAPSSLWGEGRDEGLPERATTPISQGPAQPLLQLK
                     KISSFYGQVQAHFDLDLEVRPGQIVSLLGGNASGKSTTMKVILGLLKPRAGEVWFEGR
                     SLFGLSTPQIIRRGIGSVPEARRLFPAMTVRENLLMGAYARNDRKAVAEDLGRMLELF
                     PRVAERIEFQAGTLSGGEQQMVAMARALMGRPKLLCMDEPTMGLSPLYVDKVLDLIAR
                     INRQGVSVFMVEQNASLALQIAHYGYVLQTGRIVLSGPARQLLDDARIRDAYLGGGDA
                     ARTAS"
     gene            7230..>7544
                     /locus_tag="VIO83_12075"
     CDS             7230..>7544
                     /locus_tag="VIO83_12075"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8332049.1"
                     /translation="MTLPLDHIVIAVHDLDIAILDYTALGFHVLRGGDHPGRTTHNAL
                     VVFADGSYFELIAWKAPAPEERWWQLLQRHGEGIVDFALLPRSTAATVTAAGERGLLL
                     DGP"
BASE COUNT         1098 a         2585 c         2556 g         1305 t
ORIGIN      
        1 gtggccgcgg ccttcgcgcc ctggccatcg gccaaggcgc tggccgcgct gctcgcgggc
       61 gccgacatct ggtaccacgc acgcctgagc ctgcagcgcg tgggtgtcgg gctgctgctg
      121 gctttcgcgt tcggcgtgcc gctgggcgtt ctcacgggcc tgtcgcgcag tttttcgcag
      181 gccaccacgc cggtgttcca gttcctgcgg atgatctcgc cgctttcctg gatgccgata
      241 gccgtgatgg tgctcggcgt gggtgacgca ccggtctact tcctgctcgc tttcgcggcg
      301 gtctggccga tcctgctgag cactgcggtg ggtgtggccc agctcgatgc caactggctg
      361 ctgctcgcgc gcagcctgtc ggccacgcgg cgcgagacgc tgttccgcgt gatcctgccg
      421 ggtatcacgg cgcagatcct ggccggcgtg cggctgtcca tcggcatcat ctggatcgtg
      481 ctggtgcccg ccgagatgct gggcgtgtcg gccggcctgg gctacttcat cctcgatacg
      541 cgcgaccggc tggcgtactc ggaactgatg gccgccatcg tgctcatcgg ggcgctcggc
      601 tatctcctgg atctgctggc gcgttggctg catgcgcgct ggttgcatgc ttagcggccc
      661 cgcctgacgg ccgcatcccg gtgattccgt cgagttggtc gacgtctagg ctactcgcaa
      721 gtcaggcgtg gagcaggggg gctctgccgg cgcgctgccg ctcgtagctc ttcacctgtg
      781 catacgccac cggcagcagg atcgtatcgg cggtcgacca ccggccgcga cgcaggaaat
      841 cgcccagaat gtgctcgccc tcggtgggcg cgccgcgttc gatgtccttc agcagcgacg
      901 ccgtcaggcg cgaatcgggc gccgtcacgg tcgctcgcgt gcgcgcgatc gcttccggcc
      961 gaggcgcgaa gccgttgtgg gccgcgatgc ggctgcattc gtccagcagg tccagggcca
     1021 ggccgtcggc cccggcgccg acgatgtcgc cgatgctggc gcgcatcaac gtcgtgatgc
     1081 tggcgatcgt ggcgatgtgc acccatttct cccacatctc ctgcaggatc gagtcgctga
     1141 ggcggccgac gaaggcggct tgcgcgaaag cggcggcgat cgcctcgaca cgcggcgtgc
     1201 gctcgccgtc gagttcgccg aaggccaggg tgtggtcttg gctcagatgc agcacggcgc
     1261 ctgtctcgtc gagcgtcgcg gagatcaggc ccagaccgcc cagcacccgg cggtaactga
     1321 agcgcgccgt cagcgtgtcg aggtggcgca ggccgttgag cagcggcagg acgacggttt
     1381 ccggacccac ggcagcggcg aacgacgcga tggtctcgtc gaggtcgtac gccttgcagc
     1441 cgacgatcac gacgtcgtag tgcggctgga gctgatgggc gagaacggtc ggtggcgacg
     1501 ccaggtgcag gtcgcctgca gggctgtgaa taaccaggcc gctggccgcc agttgggccg
     1561 cacgcgcggg gcgcagcagg aaggtgacat cgtgcccggc ctgaagcagc cgcccgccga
     1621 agtagcctcc aagtgcgccg gcgcccacca cgagaaatcg catccgaatc tcctttcgtg
     1681 tcgccgatgc ctgtccccgc ggttgtggtg gacgcatggc gaaagcggtg cagcctatgg
     1741 gtaattcgcc atcccggcca cgacgttttc ggcatgtgct catgcgcaaa acaacggtgg
     1801 cgagcggccg cctgtcgccc cgtgtgcgtc ggggcagccg cacttactcg ctcggcagat
     1861 atcgctgtgc ctttttcttt gttcccaagg gagggacgtc cgcgaagaat ggccgccttc
     1921 tcactcacgg agcgcctgca tgctgaatcg ccacaccttc ctgcgcatcg cggccttgct
     1981 gctggccgcg cccatggcgg cgcttgcggc acctgcatcg ggcgagccgt tgtacttcgc
     2041 cgtcagcggc ccgctgaccg gccagaacgc gcagtacggg gcacagtgga aggcgggctt
     2101 cgacctcgcg ctggacggca tcaacgccga gggcggcatc cacgggcggc cactggccta
     2161 cgtgttcgaa gacagtcaaa gcgatccgcg ccaggctgtg gggctggcgc agaagttcgt
     2221 caacgacaag cgattcctga ttgagctggg cgacttctcc agcccggcct cgatggcggc
     2281 gtcgcccatc taccagcgcg cgggtctcgt gcagttcggc ttcaccaatt cgcaccccga
     2341 cttcaccaag ggcggcgact acatctggag cccgtcgatc agccaggccg atgcgcagcc
     2401 gatcctggcc gagctggcgg tgaaggtgcg cgggctgaaa cgcgtggccg tgctcttcct
     2461 caacaccgac tggggccgca ccagcaagga cgtgttcgtg aaatccgccg aggcgctcgg
     2521 tgcgcaggtg gtcatcgccg agggctacca gccggaggag aaggacttcc ggtcaacact
     2581 ggtccgcgtg cgagacgcca agcctgacgg gctggtgctc atctcttact acgccgatgg
     2641 cgcgctgatc gccgggcagg tgcgcagcgt cggcctgacg cagccgatcg tcgcgtcgag
     2701 ctcggtgtac tcgcccaagt tcctggaact gggcggcgcg gcggtggagg gcgtcctgac
     2761 caacaccagc ttcttccccg gcgagccgcg ctccgaggtc cagcgcttcg ttcagggctt
     2821 ccgcgccgag tacggcaagg agcccgatgc cttcaatgcc tacgcctacg atgcggtgca
     2881 gttcgccgct gccgcgctgc gcgcagccgg gccgcaggca gaccgcaagg gcgtgcgtga
     2941 cgttctgtac aagatcaagg acgtgccgag cgtggtcttc ggcaaggcca ccttcgaccc
     3001 gcagacgcga cgtgtgatcg gtgttcgcag cgttcacctg gtggtcagga acgggcagtg
     3061 gggtgtcgcc gacagccaag gctccgtcgc gtcgaggtaa cgcgttctcc gcgtccgatg
     3121 accttctcct tcacctcgtt cttcgactac acggtcaacg gcctgatcat cggcaacgtc
     3181 tatgcgctgc tggccgtcgg gctggcactg atcttcggtg tctcgcacct catcaacttc
     3241 gcgcacggat cggtctacac cgtcggggcc tacatcggtt gggcctcgat cacttacctg
     3301 cacaccccgc tgccgctcac gctgctgggg gtggtgctcg gcagcggtgc gctgggcatg
     3361 ctgatcgagc gggtcgggct gcggccgctg cagggggcgg cgcgcatcgc gcccttgctg
     3421 gcgaccattg gcatcagctt cgtgctcgac atcctggtgc aactggtctt cagccccgac
     3481 ccgcgcgcac tgcccacgca gttgccggac tggcgtctgc agatcggcag cggcaccatc
     3541 ggcgcgctcg acctgctcat cgccggcatc ggcctggcga gcgccggcgt gctgttcggt
     3601 ttcctgcgtt tcaccaggct gggctgggcc gtgcgcgcca ctgcgcagga ccgtgacgcc
     3661 gcgcagcaga tgggtgtgga cgtgaaccgc gtgaaccaga cggtgttcgc catcgcctcg
     3721 gcactgggtg gcctgtccgg ggtgttggtc ggcatgtact acaacaacat cagcccggcg
     3781 atgagcttcc aggcgacgct caagggcgtg gtggcgacgg tggtcggcgg cgtgggcaac
     3841 gtgcccggcg ccatcgtggg cagcctgctg ctggggctga ccgagagcta tggcgtggcg
     3901 ctcttcggca cgccctaccg caacctcttc gccttcgtgc tgctcctgct gatcctgctg
     3961 tggaagccca atgggctctt cggtcgcagg cgtgccttgc ccccggagcc gctcaccggc
     4021 accttcattg ccccgagcag gccctggccc gtgccgcgct ggctgctggt ggcgctgttc
     4081 gcgatcgcgg ccgtgctgcc gcttgtcgaa ccgtcaccgt acgtgctgca gacgctgacc
     4141 aattcctggc tttacgcggc gctcgcgctg agcctgacac tcgtcgccgg caccatcggg
     4201 cagatctcgc tcggccatgc ggggctgctg gccatcggcg cctacgcttc gtcgctgctg
     4261 acactcgacc tccacctgcc ggtgggcacc agcatcatcg tggcgggtct gctcacctcg
     4321 gtgatcggca ccgcgctgat ctttccggcc ttccgattgc gcgggcacta cgtgtcgatc
     4381 gccacactgg gcatcggcga gatcgtggcg ctgaccatcc tcaactggga aagcctcacg
     4441 cgcgggccga tcggcgtgtc cggcattccg ccgctgtcgc tcttcgggca ggacgcgtcg
     4501 tcgaacaacg ccttctattg ggcgtccttc ggtgccatgg tgttgcttgc gctgctgcag
     4561 tggcggctgt tgcgctcgca cctggggcgg accttccgcg cggtgcgcga ggacgaggtg
     4621 gccgcgcgcg cctacggcgt gaatcccgac cgctacaagg cgatggcctt cggcttcggc
     4681 gggttcgccg ccggcgtgag cggggcgttc acggcgcatc tctactcgta catcaaccac
     4741 gagaccttca cgtcgcagat ctccatcctc gcgctgacga tggtgatcct cggtggcctg
     4801 ggcaacatca gcggcgcgct gctcggcgcg gtggcgctgg tgagcctgcc cgagctgttc
     4861 cgcgtgaccg ccgagtaccg catgctgatc tatggcatcg cgctgctgct cctcatccga
     4921 ttccgcccgc agggtctgct gggcaccgtc tgaaagaaag aacgccatga gccacctgaa
     4981 cgactacctc gcggagaagc gtgccgccgt gttggcacgc aacgccgcca tcgaggcagg
     5041 caccgcgcag cctgcgcaac tcaaggccac cgtgcgcgcc gaaggccgca gcagcgtgcg
     5101 gcggctgcgt attcgcgacc atcaggtgat cagcgacagc ccgcccgatt tcgccggcta
     5161 caacctcggc ccgagttcgc cggagctgca gttgggcgtg ttgggcacct gcgtgacgca
     5221 catctttctc atccaggcag ccgagcgcca agtgccgctg gaaagccttg aggtcgaggt
     5281 aaccggcgtg atcgatccgc gcggcggcaa gcccggccac gagcagacgc ccatctggcc
     5341 ccaccagatc ggctacacgg tgcacatcga ctcgccggcg tcgcgcgccg acatcgacgc
     5401 gctgttcgag gcggtcgagc gcaactgccc gatcctcaac ctgctgcgca acccgcagac
     5461 aatccgggcc gaggtccggc tggtgaactc ccgcgcagcg tcggtgccca cggacgcgaa
     5521 agccgccgat gccgtcgcca ctgcttgagg tcgcagcgct cacgcgccgc ttcggcgggc
     5581 tcacggccgt ggatggggtc gacttgcagg tgcgagaagg cgaactggtc agcgtgatcg
     5641 ggcccaacgg cgccggcaag accacgctgt tcaacctggt caccggcctc gaccagcccg
     5701 acggcggccg cgtgctgttc ggcgggacgg acatcacggg cctgccggcg caggagctgg
     5761 cgcggcgcgg cctggcgcgc accttccagc acggccgggt gttcgccaac ctcagcgtgc
     5821 tcgacaacgt gctggtcggc gcgcacaccc gactgcgcgc ggccaagccg gcgatgcggg
     5881 gtgtgcccgg cctggccgcc atcgccgaac tggcgctggg cctgttgcag ccgccatcgg
     5941 tcttgcggga agctcggcat ctgcgcgagc atgcgcttga gatcatcgcg ctcttcggcg
     6001 accggctgac gcctcgcatc catcagccgg cgcacagcct gtcgtacgcc aaccgccggc
     6061 gcctcgagat cgcgcgtgcc ctggcgctgc agccgcagct gctgctgctc gacgagccga
     6121 ctgccggcat gaacgagagc gaaacggtcg agatgctgca aatcatccgt ggactcaagc
     6181 agcgcggcga gaccatcctg ttgatcgagc acaagctcga cttggtgatg cagctgtctg
     6241 accgggtggt ggtgctcgac gacggccgca agatcgcgga aggcttgccc gagcaggtgc
     6301 gccacgaccc ggcggtgatc gaggcctacc tggggcatcg ccacatcggc gaacaagtgg
     6361 tggccgcatg atcgcgaccg tcgcgcgcag cggactggcg ccctcatcgc tttggggcga
     6421 gggccgggat gaggggctgc ccgaacgcgc cacaacgccc atttcccaag gccccgcaca
     6481 gcccctgctt caactgaaga agatcagcag tttctatggt caggtccagg cgcacttcga
     6541 cctcgacctg gaagtccggc ccggccagat cgtgagcctg ctgggcggca acgccagcgg
     6601 caagtcgacc accatgaagg tgatcctcgg ccttctcaag ccccgcgcgg gcgaggtgtg
     6661 gttcgaaggt cggtcgctgt tcggtttgtc gacgccgcag atcatccgcc gcggcatcgg
     6721 ctcggtgccg gaggcgaggc gactgttccc cgcgatgacg gtgcgcgaga acctgctgat
     6781 gggcgcctat gcgcgcaacg accgcaaggc cgtggccgag gacctgggcc gcatgctgga
     6841 actcttcccg cgcgtggccg agcgcatcga gttccaggcc ggcacgctct cgggcggcga
     6901 gcagcagatg gtggccatgg cgcgtgccct catgggtcgg cccaagctcc tgtgcatgga
     6961 cgagccgacc atggggctgt cgccgctcta cgtcgacaag gtactcgacc tcattgcccg
     7021 catcaaccgg cagggcgtct cggtcttcat ggtcgagcag aacgccagcc tggccttgca
     7081 gatcgcgcac tacggctacg tgctgcagac cggccgcatc gtgctttccg gtccggcgcg
     7141 gcaactgctc gacgatgcgc gcatccgcga tgcctacctc ggtgggggcg atgccgcccg
     7201 caccgcctcc tgaaactgaa aggcacacca tgacccttcc gctggatcac atcgtcatcg
     7261 cggtccacga cctcgacatt gccatcctcg actacaccgc gctcggcttt cacgtgctgc
     7321 gcggcggcga ccatcccggc cgcacgaccc acaacgcgct cgtcgtcttt gccgatggca
     7381 gctacttcga gctgatcgcc tggaaggcgc cggcgccgga ggagcgctgg tggcaactgc
     7441 tgcagcgcca cggggagggc atcgtcgact tcgcgctgct gccgcgcagc accgccgcca
     7501 cggtgaccgc cgcgggtgaa cgcggcctgc tgctggacgg gccg
//
LOCUS       DATCUN010000322         1893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_54774, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000322 DATCUN010000000
VERSION     DATCUN010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54774"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1893)
                     /locus_tag="VIO83_12080"
     CDS             complement(<1..>1893)
                     /locus_tag="VIO83_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003108594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY8332050.1"
                     /translation="VIDQRLAAQPASPTAALLAIRVIDPACGSGHFLLAAARRLAEKL
                     AELRSLEGGQEGAIQPQDYRHALREVVARCIFGVDRNPMAIELARMALWLEGYEEGRP
                     LGFLDHHLQVGDALLGLTDLNVLEQGIAKDAFKPLSGDDKDVCKELAKANAAGLKQIA
                     KDLQGKQMLLGVDNRSGLEALRAIETLPADTPEQVAAKEQAWRHFLEDSAQSPLAHAA
                     DALVGAYLLPKTEDTAETVPTSITLHALLTDPQRAQTEHAAPIAAARAACEQARVFHW
                     PLAFPQVFAQGGFDCVLGNPPWERIKLQEEEFFATRHRDVAQARNKAERAQRIQWLSE
                     GMLARHLYPDLAHETHEDEAEKRLYREFVTARRAAEAASVFAHVKGADGGRYPLTGVG
                     DVNTYALFAETMLQIADSGGRAGFIVPSGLGTDDSTKRFFNSLVRNNRLVSFFGFDNA
                     KKIFPSVHPDTPFALVTLGATEFDPELAHYLLGIEHLADPRRRFTLTPEEFRLINPNT
                     LTCPVFRSERDAELTKKLYRAAPVLMRDAVIAGEGKQAKVVEPAQNPWGISFIRMLDM
                     ANDSHLFKTEPAPDRLPLYEAKLIHQFDHRWATYTPDGNSRDVTPAEKADPAFTVTPR
                     YWVEAR"
BASE COUNT          286 a          559 c          677 g          371 t
ORIGIN      
        1 tcgcgggcct ctacccagta gcgcggggtg acggtgaaag ccgggtcggc cttttccgcc
       61 ggcgtgacat cacggctgtt gccgtctggc gtgtaggtgg cccagcggtg gtcgaactgg
      121 tggatcagtt tggcctcgta cagcggcagg cggtcggggg ctggctcggt cttgaacaga
      181 tggctgtcgt tcgccatgtc cagcatgcga atgaacgaaa taccccacgg gttttgcgcg
      241 ggttccacca ccttggcctg cttgccctcg cctgcgatca cagcatcgcg catcagcacc
      301 ggggcggcgc ggtagagctt cttggtcagc tccgcgtcgc gctcgctgcg gaacaccggg
      361 caggtcagcg tgttggggtt gatgaggcgg aattcctcgg gcgtgagggt gaagcggcgg
      421 cgcgggtcgg ctaaatgttc gatgccgagc agatagtgag caagctccgg atcgaattcc
      481 gttgccccaa gagtcaccaa agcgaatggt gtatctggat gaactgaagg aaagattttc
      541 ttggcattat caaagccaaa gaaggaaaca agtcgattgt ttcttaccaa gctattgaag
      601 aagcgcttgg tagagtcgtc tgtcccaaga ccggagggga caataaatcc agctcgtcca
      661 ccggaatcag caatctgcag catcgtttcg gcaaacagcg cgtaggtgtt cacatcgccc
      721 acgccggtca gcgggtagcg gccgccgtcg gcgcctttca catgggcgaa cacgctggcg
      781 gcttccgccg cgcggcgggc ggtgacgaac tcgcggtaga ggcgtttttc tgcttcgtcc
      841 tcgtgcgttt cgtgggccag gtcggggtac aggtggcgtg ccagcatgcc ttcgctgagc
      901 cactggatac gctgggcgcg ttcggccttg ttgcgggctt gcgccacgtc gcggtggcga
      961 gtggcgaaga attcttcctc ttgcagcttg atgcgctccc acggcggatt gcccagcacg
     1021 cagtcgaagc cgccctgcgc aaacacctgc gggaaggcca gcggccagtg gaacacgcgg
     1081 gcctgttcgc aggccgcgcg tgcggcggcg atgggcgcgg cgtgctcggt ctgggcgcgt
     1141 tgcgggtcgg tcagcagcgc atgcagggtg atgctggtgg gcacggtttc tgccgtgtcc
     1201 tccgtcttgg gcagcaggta ggcgcccacc agcgcatcgg cggcgtgggc cagcgggctc
     1261 tgggcggagt cctccagaaa gtggcgccag gcttgttcct tggcggccac ctgttcgggc
     1321 gtgtcggcgg gcagggtttc gatggcgcgc agggcttcca ggccgctgcg gttgtccacg
     1381 cccagcagca tctgcttgcc ttgcaggtct ttggcgatct gcttgaggcc ggcggcgttg
     1441 gccttggcca gttccttgca gacatccttg tcgtcgccgc tcaatggttt gaaggcatcc
     1501 ttggcgatgc cttgttccag cacgttcagg tcggtcaggc cgagcagcgc gtcgcccact
     1561 tgcaggtggt ggtcgagaaa gcccagcggt cggccttcct cgtagccttc cagccacagg
     1621 gccatgcgcg ccagttcgat ggccatggga ttgcggtcca cgccgaagat gcagcgggcc
     1681 acgacttcgc gcagggcatg gcggtagtcc tgcggctgga tggcgccttc ctgcccgcct
     1741 tcgaggctgc gcagctcggc cagtttttcc gccaggcggc gggcggcggc gagcaggaag
     1801 tggccgctgc cgcaggccgg gtcgatgacg cggatggcca gcagcgcggc ggtggggctg
     1861 gccggctggg cggccagacg ctggtcgatg acc
//
LOCUS       DATCUN010000323         5969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_35667, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000323 DATCUN010000000
VERSION     DATCUN010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35667"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VIO83_12085"
     CDS             <1..687
                     /locus_tag="VIO83_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HEY8332051.1"
                     /translation="AMLGYVVALISFPVEMTSWPAPRGFEAVSPTLGLLEGLQRILGL
                     SANLPDAWTQATALDALKTNKSLTIAELWAGNPAFGSLGGYGSEAVNLAFLAGGLFLL
                     YKRVFTWHAPVGMLAAMALMSLLFWNGAGSDSNGSPLFHLLTGATMLGAFFIVTDPVS
                     GATSVLGRLIFGAGVGVLAYIIRTWGGYPDGVAFAVLLMNLTAPTIDYYTRPRTYGHR
                     QPERGFKLGD"
     gene            692..1354
                     /gene="rsxG"
                     /locus_tag="VIO83_12090"
     CDS             692..1354
                     /gene="rsxG"
                     /locus_tag="VIO83_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HEY8332052.1"
                     /translation="MLPEMPRSMLRNALILGLFAVATVGVVAITQQGTATRIQAAERE
                     AQVRALAEILPADSYDNHLLDNSVQLSDPLLGSARPLPAYLALKGGQPSAIILQAIAP
                     DGYSGSIRLLIGIHADGRLAGVRVLAHKETPGLGDKIELAKSPWVLAFNGKSLSDPDD
                     NGWGVKKDQGVFDQFAGATITPRAVVKAVHHALQYFDGHKAELFAQHAAAQPTPEQER
                     AQ"
     gene            1351..2064
                     /locus_tag="VIO83_12095"
     CDS             1351..2064
                     /locus_tag="VIO83_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HEY8332053.1"
                     /translation="MSQSYREIAVNGLWKNNPGLVQLLGLCPLLGTSNSTVNALGLGL
                     ATALVLTCSNAAVSMIRGVTQTAVRLPAFVMIIAALTTCIELLMQAFTYELYQILGIF
                     IPLITTNCVILGRADGFAAKNGIARSAFDGLMMGLGFALVLLALGAVRELLGTGVLFA
                     NMQLLFGPIAADWQLTVFSDYKGFLLAILPPGAFLVLGLLIAGKNILDARAEARAKAL
                     QPAAEPAASRRVRVTGVVE"
     gene            2061..2699
                     /gene="nth"
                     /locus_tag="VIO83_12100"
     CDS             2061..2699
                     /gene="nth"
                     /locus_tag="VIO83_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY8332054.1"
                     /translation="MNAAKRAEIFRRLKEDNPEPTTELEYQSIFELLVAVILSAQATD
                     VGVNKATARLFPVANTPAAIHALGVEGLEAYIKTIGLYRSKAKNVIETCRLLVERHAG
                     EVPSTREELEALPGVGRKTANVVLNTAFRQPAMAVDTHIFRVSNRTGIAPGKTVLEVE
                     KKLLKFVPKEYLLDAHHWLILHGRYVCKARKPQCGSCRIEDLCEYKHKTSDD"
     gene            2810..2995
                     /locus_tag="VIO83_12105"
     CDS             2810..2995
                     /locus_tag="VIO83_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017738282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8332055.1"
                     /translation="MAQAKTDLELEEDFVVDAEPEDAEAKDAPTSKTNLAKRRVIDNL
                     LEEKRLKKQLAEYDFDL"
     gene            complement(3098..3799)
                     /locus_tag="VIO83_12110"
     CDS             complement(3098..3799)
                     /locus_tag="VIO83_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8332056.1"
                     /translation="MAMQSFPDGYRALVIGASGAIGGAFVAALRDDPRCALVVGLDRH
                     SQPAIDFGREESIAEAATVLAAQGPFQLIINAAGILHGEAFMPEKKLADLHYDQLLET
                     FRVNTFGPALVVRHFSRLLDRQRSVLALLSAKVGSIEDNRLGGWYSYRASKAALNMLV
                     KTAAIEVRRSNPGALLLALHPGTVNSRLSQPFRGAEIGRPPALAAQQMLSVIDGLGAE
                     ASGQFHAWNGEWLPW"
     gene            complement(3800..4327)
                     /locus_tag="VIO83_12115"
     CDS             complement(3800..4327)
                     /locus_tag="VIO83_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015635050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 domain-containing protein"
                     /protein_id="HEY8332057.1"
                     /translation="MLKKFLLPLCLLLASCGQIEVEHYRDQQPQLDLRQYFSGRVDAW
                     GMFQKRSGEVIKRFHVVINGYAQGDKLILDEDFTYSDGSKQKRVWTLHPDGPGRWRGT
                     ADDVVGEARGEVAGNALRWRYVLSLPVDGKVYQVDFDDWMYLLDENTMANRSFMTKFG
                     FELGQVTLFFRKQVQ"
     gene            complement(4338..4880)
                     /locus_tag="VIO83_12120"
     CDS             complement(4338..4880)
                     /locus_tag="VIO83_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008044945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEY8332058.1"
                     /translation="MNDPASRQGTSKAWLLLLCLLSTGALANWQEAIPEAQVVGAGEL
                     RLFGLRIYSARLWSPAQPLATDTPFALELTYHRAISRDDLVDASVKEIRRLSGGAVSA
                     AQLAGWRAQMRQAFVDVEPGARITGVHLPGRGARFYVGQQLQHEVRDAAFAAAFFAIW
                     LDPRTRNPELRAQLLGTDAP"
     gene            complement(4967..>5969)
                     /locus_tag="VIO83_12125"
     CDS             complement(4967..>5969)
                     /locus_tag="VIO83_12125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HEY8332059.1"
                     /translation="WLHVEHEGRGLTLIAGKLLAERSQMLAAYRLKLWLALAAGAVLA
                     FLLGWLVSLRGLRPLRELAARARAIDVQHLHLRLDEVGEVSELRSLSAALNQMLARLE
                     DGFAQLSRFSEDLAHEMRTPLSNLMGHTQQTLRHPRSVEDYQNLLASNQEEYERLARM
                     IDSMLFLARTEQPQASVKREAVDLRGLVEQLCEYFEGMAEERELQLVNRAEGTLRADQ
                     GLLRRALANLLANALRHATPGSPVSIASQLCDGRLEVGVHNLGEPIGAEHLPRLFERF
                     YRCDPSRAQPGDSGGLGLAIVRSIMQLHGGSVRVESGEQGTRFILVFPLADRLNHKQ"
BASE COUNT         1037 a         2119 c         1902 g          911 t
ORIGIN      
        1 gcgatgctcg gctacgtggt ggcgctgatc tccttcccgg tggaaatgac cagctggccg
       61 gcgccgcgcg gcttcgaggc ggtcagcccg accctcggcc tgctcgaggg cctgcagcgc
      121 atcctcggcc tgagcgccaa cctgccggac gcctggaccc aggccaccgc gctggacgcc
      181 ctgaagacca acaagagcct gaccatcgcc gagctgtggg ccggcaatcc ggcgttcggt
      241 agcctgggcg gctacggcag cgaggcggtc aacctggcct tcctcgccgg cggcctgttc
      301 ctgctctaca agcgggtgtt cacctggcat gcgccggtgg gcatgctcgc cgccatggcg
      361 ctgatgagcc tgctgttctg gaacggcgcc ggctccgact ccaacggctc gccgctgttc
      421 cacctgctga ccggcgcgac catgctcggc gccttcttca tcgtcaccga cccggtgtcc
      481 ggcgccacca gcgtgctcgg ccggctgatc ttcggcgccg gcgtcggcgt gctggcctac
      541 atcatccgca cctggggcgg ctacccggac ggcgtagcct tcgccgtgct gctgatgaac
      601 ctgaccgcgc cgaccatcga ctactacacc cgtccgcgca cctacggcca tcgccagccg
      661 gaacgcggct tcaagctggg ggactgaggc catgctgccg gaaatgccgc gttcgatgct
      721 gagaaacgcc ctgatcctcg gcctgttcgc cgtcgccacc gtcggcgtcg tggcgatcac
      781 ccagcagggt accgccacgc gcatccaggc cgccgagcgc gaggcccagg tgcgcgcgct
      841 ggccgagatc ctgccggcgg acagctatga caaccacctg ctggacaaca gcgtgcagct
      901 cagcgatccg ctgctcggca gcgccaggcc gctgccggcc tacctggcgc tgaagggcgg
      961 ccagcccagc gccatcatcc tccaggccat cgccccggac ggctacagcg gcagcatccg
     1021 cctgttgatc ggcatccacg ccgacggccg cctggccggc gtgcgcgtgc tcgcccacaa
     1081 ggaaaccccg ggcctcggcg acaagatcga actggccaag agcccctggg tactggcgtt
     1141 caacggcaag tcgctgagcg atccggacga caacggctgg ggcgtgaaga aggatcaggg
     1201 cgtgttcgac cagttcgccg gcgccaccat caccccgcgc gcggtggtca aggcggtgca
     1261 ccacgccctg cagtatttcg acgggcacaa ggccgagctg ttcgcccagc acgccgccgc
     1321 ccaaccgacg cccgaacagg agcgcgcgca atgagccaga gctatcgcga gatcgccgtc
     1381 aacggcctgt ggaagaacaa ccccggcctg gtccagctgc tcggcctgtg cccgctgctc
     1441 ggcaccagca actcgacggt caacgccctc ggcctcggcc tggccaccgc gctggtgctg
     1501 acctgctcca acgccgcggt gtcgatgatc cgcggcgtga cccagaccgc cgtgcgcctg
     1561 ccggcgttcg tgatgatcat cgccgccctg accacctgca tcgaactgct gatgcaggcg
     1621 ttcacctacg agctgtacca gatcctcggc atcttcatcc cgctgatcac caccaactgc
     1681 gtgatcctcg gccgcgccga cggcttcgcg gcgaagaatg gcatcgcccg ttcggcgttc
     1741 gacggcctga tgatgggcct gggcttcgcc ctggtgctgc tggcgctcgg cgccgtgcgc
     1801 gagctgctcg gcaccggcgt actgttcgcc aacatgcagc tgctgttcgg ccccatcgcc
     1861 gccgactggc agctcaccgt gttcagcgac tacaagggct tcctgctggc gatcctgccg
     1921 ccgggcgcct tcctggtgct gggcctgctg atcgccggca agaacatcct cgatgcgcgt
     1981 gccgaggcgc gggccaaggc cctgcagccg gcggcggagc cggcagcgag ccgtcgcgta
     2041 cgcgtcaccg gggtagtgga atgaatgcgg ccaagcgcgc ggaaatcttc cgccgcctga
     2101 aggaagacaa tccagagccg accaccgagc tggaatacca aagcatcttc gagctgctgg
     2161 tggcggtaat cctctccgcc caggccaccg acgtcggggt caacaaggcc accgccaggc
     2221 tgttcccagt ggccaacacc cccgccgcca tccatgcgct gggcgtcgag ggcctcgagg
     2281 catacatcaa gaccatcggc ctgtaccgca gcaaggcgaa gaacgtcatc gagacctgcc
     2341 gcctgctggt cgagcgccac gccggcgagg tgccgagcac ccgcgaggag ctggaagccc
     2401 tgcccggcgt cggccgcaag accgccaacg tggtgctcaa caccgccttc cgccagccgg
     2461 ccatggcggt ggacacgcac atcttccggg tcagcaaccg cacaggcatc gcgccgggca
     2521 agaccgtgct ggaagtggaa aagaagctgc tcaagttcgt gccgaaagag tatctactcg
     2581 acgcccacca ctggctgatc ctgcacggcc gctacgtctg caaggcccgc aagccgcagt
     2641 gcggcagctg ccggatcgag gatctgtgcg agtacaagca caagacctcc gacgattgag
     2701 ccaataaaaa taattcctat caaccgatag aaaaaatctc ttttacccac ccctgctttg
     2761 ccgctataag gtgcgcgaaa cgccctctgt tctagtggag cagcatgcca tggcacaagc
     2821 taagactgac ctcgaactgg aagaagactt cgtcgtcgac gccgaaccgg aagacgccga
     2881 agccaaggat gccccgacca gcaagaccaa cctggccaag cgccgggtga tcgacaacct
     2941 gctggaagaa aagcgcctga agaagcagct cgccgaatac gacttcgacc tgtagtcccc
     3001 gcccgatgag ccccgccaac ctgcggggct tgtgttttgc caccacaccc gccccctcga
     3061 ccggccgcca ggccaggcgt agagcccggc tgggcgctta ccagggcagc cattcgccat
     3121 tccaggcatg gaactgcccg ctggcctcgg cacccaggcc gtcgatcacc gagagcatct
     3181 gctgcgccgc cagggccggt gggcgaccga tctcggcacc gcgaaacggc tgcgacagcc
     3241 gcgagttcac cgtccccgga tgcagggcca gcagcagcgc gccggggttg ctgcgccgca
     3301 cctcgatcgc cgcggtcttc accagcatgt tcagcgcggc cttggaagcc cggtagctgt
     3361 accagccgcc caggcgattg tcctcgatgc tgccgacctt ggccgacagc agcgccagca
     3421 cgctgcgctg gcgatccagc aagcggctga aatgacgcac gaccagcgcc gggccgaagg
     3481 tattgaccct gaaggtttcc agcagctggt cgtagtgcag gtcggccagc ttcttctccg
     3541 gcatgaacgc ctcgccatgc aggataccgg cagcattgat gatcagctgg aacggcccct
     3601 gcgccgccag gaccgtcgcg gcctcggcga tgctctcttc gcggccgaaa tcgatggccg
     3661 gctgcgaatg gcggtccagg cccaccacca gcgcgcagcg cggatcgtcg cgcaacgccg
     3721 cgacgaaggc gccgccgatg gccccggagg cgccgatcac cagggcgcga tacccgtcgg
     3781 gaaacgattg catggccatt cactgcacct gcttacggaa gaagagggtc acctggccga
     3841 gctcgaagcc gaacttggtc atgaacgaac ggttggccat ggtgttctcg tcgagcaggt
     3901 acatccagtc gtcgaaatcg acctggtaga ccttgccgtc caccggcagg ctcagcacgt
     3961 agcgccagcg cagcgcattg ccggccacct cgccgcgcgc ctcgccgacc acatcgtccg
     4021 cagtgccgcg ccagcgtccc gggccgtcgg gatgcagggt ccacacccgc ttctgcttgc
     4081 tgccgtcgct gtaggtgaag tcctcgtcga ggatcagctt gtcgccttgc gcatagccgt
     4141 tgatcaccac atggaagcgc ttgatcacct cccccgagcg cttctggaac atgccccagg
     4201 catccacccg gccgctgaag tactggcgca ggtcgagctg cggctgctgg tcgcgatagt
     4261 gctccacctc gatctgcccg caactggcga gcagcaggca cagcggcagc aggaatttct
     4321 tcagcatggc aatttcctca aggggcgtcg gtgcccagca gttgcgcgcg cagctccgga
     4381 ttgcgcgtgc gcgggtcgag ccagatggca aagaaggcag cggcgaaagc agcgtcgcgc
     4441 acctcgtgct gcaactgctg gccgacatag aagcgcgccc cccggcccgg caagtgcacg
     4501 ccggtgatcc gcgcgcctgg ctccacgtcg acgaacgcct ggcgcatctg cgcccgccag
     4561 ccggccagct gcgccgcgct caccgcgccg ccggacagcc ggcggatctc ctttacgctg
     4621 gcatcgacca gatcgtcgcg gctgatggcg cggtggtagg tgagttccag ggcgaagggg
     4681 gtgtctgtcg ccaatggctg cgcgggactc cacagccgcg cgctgtagat gcgcaggccg
     4741 aacaggcgca actcgccggc accgaccacc tgggcctcgg ggatggcctc ctgccaattg
     4801 gccaacgcgc cagtacttag caggcagagc aacagcagcc aggctttgct ggtcccctgg
     4861 cgggacgctg ggtcattcat cgcaggctcc tcgtcgcctt gccacaggca gggcgagccg
     4921 taaggtcacc cctcaaaacc tatacgataa ataaatattt gtacaattac tgtttatgat
     4981 tcaggcggtc ggcgagcgga aaaaccagaa taaagcgggt gccctgctcg ccgctttcga
     5041 cccgcacgct gccgccatgc agctgcatga tcgagcgcac gatcgccagg cccaagccgc
     5101 cggagtcgcc aggctgggca cgtgaagggt cgcagcgata gaaacgctcg aacaggcgcg
     5161 gcagatgctc ggcgccgatc ggctcgccca gattgtgcac gccgacttcg agccgcccgt
     5221 cgcaaagctg gctggcaatg ctcaccggac tgcccggcgt ggcatggcgc agggcgttgg
     5281 ccagcaggtt ggccagggcc ctgcgcagca ggccctggtc ggcccgcagg gtgccctcgg
     5341 cccggttgac cagctgcagc tcgcgctcct cggccatacc ctcgaagtac tcgcacaact
     5401 gctcgaccag cccgcgcagg tcgaccgcct cgcgcttcac cgaggcctgc ggctgctcgg
     5461 tgcgagcgag gaacagcatg ctgtcgatca tccgcgccag acgctcgtac tcctcctggt
     5521 tggaggccag cagattctgg tagtcctcga ccgagcgggg gtggcgcagg gtctgctggg
     5581 tgtggcccat caggttgctc agcggcgtgc gcatctcgtg ggcgagatcc tcggaaaagc
     5641 gcgacagctg ggcgaagcca tcctccagtc gcgccagcat ctggttcagc gccgcgctca
     5701 agctgcgcag ctcgctgacc tccccgacct cgtcgaggcg caggtgcagg tgctgcacgt
     5761 cgatggctcg cgcccgcgcc gccagctcgc gcagcggacg caggccgcgc aggctgacca
     5821 gccagccgag caggaaggcc agcacggccc cggcggccag cgccagccag agtttcagcc
     5881 ggtaggcggc gagcatctgg ctgcgctcgg cgagcagctt gccggcgatc agggtcaggc
     5941 cgcggccctc gtgctcgacg tgcagccag
//
LOCUS       DATCUN010000324         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_35742, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000324 DATCUN010000000
VERSION     DATCUN010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35742"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1897)
                     /locus_tag="VIO83_12130"
     CDS             complement(<1..1897)
                     /locus_tag="VIO83_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY8332060.1"
                     /translation="MNIPTLREWLYSAKALIAALLALYIALALPLDNPYWAMASVYVV
                     SHPLSGATRSKALFRALGTLLGAAAAVVMVPPFAHQPVMQSLVISLWVGVLLFLSLLD
                     RSPRSYIFMLAAYTVPLIALPQVEHPGAIFDAALARSEEILLGIACAAVVNAVLFPSR
                     IGPVIAARMDALLGDARAWIAGSLNPQQFGEAAPRTLHKLLGDVMALDGMIMQLSYDS
                     ASHLPARHAREFRARMAMLLPQLSSLIDPLHRLHSELGQLPAELSELLARLEAWLQSG
                     DAEGGRQLREHSLRLETWLSDSHPPQALLIGSALRQLRQLLVLWQDCLNLRQSFAEDH
                     PEQPPTLHYKVRQLIGGPRYYDYGLLAFSAASVGLSVFVACLLWIAVGWDYGFSGIFL
                     VAVASCFFAAQDNPAPFIKTFLNWTLISTVAAGVYLFGLLPNIHDFGMLAIALALPLL
                     LAGTLSGRPQFTVAVMLFAVQTISTITIQDAYKANFPLFADVALSNILGLVFAIVWAR
                     VSKPFGAEWAARRLARSTWRDLADLAQSEREHDHDRVAARMLDRTSQLVPRLTLVSDP
                     RLALTDITRDLRVCFRLLDLQRSLPVAIRRQVNPVLQEVHDYFQGCADAHHQLPPPER
                     LRARLDAS"
     gene            2088..>2487
                     /locus_tag="VIO83_12135"
     CDS             2088..>2487
                     /locus_tag="VIO83_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8332061.1"
                     /translation="MKSIPNYALYGEAAPPVWCDLLHVEWIRERSPHYQGEIRPHRHD
                     ALLQIFYVRRGSGEVSIENRRMSFAAPCLILLPCRTVHAFSHDQDTDGPVITAVQQPL
                     ESMARIVSVELLGLLQRPALIPLSWPADGHE"
BASE COUNT          419 a          787 c          942 g          339 t
ORIGIN      
        1 ggctggcgtc caggcgggcg cgcaggcgct cgggcggcgg cagctggtgg tgggcgtcgg
       61 cgcagccctg gaagtagtcg tgcacctcct gcagcaccgg gttcacctgg cggcggatgg
      121 cgacgggcag gctgcgctgc aggtcgagca ggcggaagca cacgcgcagg tcgcgggtga
      181 tgtcggtcag cgccaggcgc gggtcgctga ccagggtcag gcgcggcacc agctggctgg
      241 tgcggtcgag catgcgcgcg gcaacgcggt catggtcgtg ttcgcgctcg ctctgcgcca
      301 ggtcggcgag gtcgcgccag gtcgagcggg ccaggcggcg ggcggcccac tcggcgccga
      361 agggtttgct cacccgggcc cagacgatgg cgaacaccag gccgaggatg ttgctcaggg
      421 cgacatcggc gaacagcggg aagttggcct tgtaggcgtc ctggatggtg atggtggaga
      481 tggtctgcac ggcgaacagc atcaccgcca cggtgaactg cgggcgcccg gagagggtgc
      541 cggccagcag cagcggcagc gccagggcga tggccagcat gccgaagtcg tggatgttgg
      601 gcagcaggcc gaacaggtag acgccggcgg cgacggtgga gatcagcgtc cagttgagga
      661 acgtcttgat gaacggcgcc ggattgtcct gcgcggcgaa gaagcagctg gccaccgcga
      721 cgaggaagat gccgctgaaa ccgtagtccc agcccacggc gatccacagc aggcaggcga
      781 cgaacaccga caggcccacc gaggcggcgg agaaggccag caggccgtag tcgtagtagc
      841 gcggcccgcc gatcagctgg cgcaccttgt agtgcagggt cggcggctgc tccgggtggt
      901 cttcggcgaa gctctgccgc aggttcaggc agtcctgcca gagcaccagc agctggcgca
      961 gctggcgcag ggcgctgccg atcagcaacg cctgcggcgg gtggctgtcg ctgagccagg
     1021 tctccaggcg caggctgtgc tcgcgcagct ggcggccgcc ctcggcatcg ccgctctgca
     1081 gccaggcctc caggcgcgcg agcagttcgc tcagctccgc cggcagctgg cccagctcgc
     1141 tgtgcaggcg gtgcagcggg tcgatcagcg agctcagctg cggcagcagc atggccatgc
     1201 gcgcgcggaa ctcgcgggca tgccgggccg gcaggtggct ggcgctgtcg taggacagct
     1261 gcatgatcat gccgtccagg gccatcacgt cgccgagcag cttgtgcagg gtgcgcggcg
     1321 ccgcttcgcc gaactgctgc gggttcaggc tgccggcgat ccaggcgcgg gcgtcgccga
     1381 gcagggcgtc catgcgcgcg gcgatcaccg ggccgatgcg gctggggaac agtacggcgt
     1441 tgaccaccgc ggcgcaggcg ataccgagga ggatttcctc ggagcgcgcc agggcggcgt
     1501 cgaagatggc gcccggatgc tcgacctggg gcagggcgat cagcggcacg gtgtaggccg
     1561 ccagcatgaa gatgtagctg cgcgggctgc ggtcgagcag cgagaggaac agcagcacgc
     1621 cgacccacag cgagatgacc aggctctgca tcaccggctg atgggcgaac ggcggcacca
     1681 tgaccaccgc ggcggcggcg ccgagcaggg tgccgagggc gcggaacagc gccttggaac
     1741 gggtggcgcc ggacagcgga tgggacacaa cgtacaccga cgccatcgcc cagtaggggt
     1801 tgtcgagggg cagcgccagg gcgatgtaga gcgcgagcaa ggcggcgatc agcgctttgg
     1861 ccgaatacag ccattccctc aaggtaggga tgttcatcga tgcggaaagc tcggacagca
     1921 tgccggcggg cggcatggga gacgcgggga ggtagactta attaccatgt gttgcccatt
     1981 aaacgtcagt gcgccagaaa aaacggcact ttgaacagga caatccgtgc aggttggcct
     2041 gggcaagacg tccgtcgctg gccgcgccgc ctccacgagc gatcccgatg aagagcattc
     2101 ccaactatgc cctgtacggc gaggcggccc cgccggtgtg gtgcgacctg ctgcacgtcg
     2161 agtggattcg cgaacgcagc ccgcactacc agggcgagat ccgcccgcac cgccacgacg
     2221 ccctgctgca gatcttctac gtgcgccgcg gcagcggcga agtcagtatt gagaataggc
     2281 ggatgagctt cgccgcgccc tgcctgatcc tgctgccctg ccgcacggtg catgccttca
     2341 gccacgacca ggacaccgac gggccggtga tcaccgccgt gcagcagccg ctggagtcga
     2401 tggcgcggat cgtctcggtc gagctgctcg gcctgctgca gcgcccggcg ctgattccgc
     2461 tgtcctggcc ggccgacggc cacgagc
//
LOCUS       DATCUN010000325         2919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_224033, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000325 DATCUN010000000
VERSION     DATCUN010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224033"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VIO83_12140"
     CDS             <1..292
                     /locus_tag="VIO83_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3742 family protein"
                     /protein_id="HEY8332062.1"
                     /translation="RLAGRLVARGLPPGAATALLWLVKLAVLGVLLYAAFWLALLLVF
                     AVAAAWAMQHGTADQEEPDLLNRQAEERDHREGLFYHPASYNDDPDPRFEDD"
     gene            complement(304..1830)
                     /locus_tag="VIO83_12145"
     CDS             complement(304..1830)
                     /locus_tag="VIO83_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HEY8332063.1"
                     /translation="MTLYTTDYLEYYLTLVSWIVHNGVWNVLVASGVFAIPFLAIVIQ
                     EWLKARAEGADEGNKGVLSSARIENRVFVAIVVIMFAGIPFIDVDFTTLQFDRSRSAQ
                     CQVNVPEPSETGWSQSFSTLNNQSARVPVWWFFMHAISRAVTGASVAAIPCGTDLRQI
                     RMDIDNTRIADPVLAQEVADFTHNCYGPARARLFMDRPDLTEEQMHDVTWIGSRFFVD
                     TPGFYDTYRSRSPREGWPYDSNRDAGLAEVPSGAGYPTCRQWWSDGNGLRSRLLQQVD
                     PSLLTRVAGWAGFLSRDQVDDSVIRAIASPRQQKLNQGAVYSDYGGQIEMTLPNIVTR
                     AASDVGMTVGALGFFPAMDVMRQALPMVLALLKMALVICIPLALLVGTYDLKTLVTVS
                     VVQFALFFVDFWFQLARWIDSTILDALYGWGFGWNRPHSNFDPLIGLNNAFGDMLLNY
                     VLATMFLVLPAFWVTALAWTGIRAGNFLQGLAAGTRDAAQAGGRGSGILISAASPGKK
                     "
     gene            complement(1844..2185)
                     /locus_tag="VIO83_12150"
     CDS             complement(1844..2185)
                     /locus_tag="VIO83_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8332064.1"
                     /translation="MSWVAVRRYSARALLALLVLVLVLIAAAVVNLVGIRMAGGIDGW
                     RQWMDAHAGWFLVWRLLLYAVTVYGWLWMRRRLHAREPEGGARQRLLRVEVAAVLAVV
                     ALEVSQFMQAG"
     gene            complement(2182..>2919)
                     /locus_tag="VIO83_12155"
     CDS             complement(2182..>2919)
                     /locus_tag="VIO83_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HEY8332065.1"
                     /translation="PVRVVGDVTRAGYNLLNGRDVAATGSIDPGSCNGNLACETWRSP
                     DDAAAWAVRVLGEQEQRTCESCTKTTTTPGVGLTPLIQEEYEAKLQALQELVAGSRPT
                     SFANLQDASAASLPITRGVIEALRDEPDQDILARRLASEVALASVLEKALLLQRTLLT
                     GRKEPNVSANELAQKAVSHESDILDREILNLKTELELRRELASNSPTAIIQRHSTRST
                     GSRGIYEGDPTRNRIDELQRPPSGNNP"
BASE COUNT          495 a         1000 c          950 g          474 t
ORIGIN      
        1 ccggctcgcc ggccggttgg tagcgcgcgg tctgccccca ggcgcggcga cggcgctgct
       61 gtggctcgtg aagctggccg tgctgggagt actgctgtac gccgcgttct ggctggcgct
      121 cctgctggta ttcgcggtcg ccgccgcctg ggccatgcag cacggcaccg cggaccagga
      181 agaacccgac ctgctgaatc gccaggccga ggaacgcgat caccgcgaag gcctcttcta
      241 ccaccccgca tcctacaacg acgatcccga tcctcgattc gaggacgact gatcccggtc
      301 gcgctacttc ttgccgggac tcgccgcact gatcaggatg cctgagcccc tgcctcccgc
      361 ctgcgccgca tccctggtgc ccgcggccag tccctgcaag aagttgccgg cccggatgcc
      421 cgtccaggcc agtgccgtca cccagaacgc ggggagcacc aggaacatcg ttgcgagcac
      481 gtagttgagc aacatgtcgc cgaatgcgtt gttcagcccg atcaatggat cgaaattgct
      541 gtgcggccga ttccagccga atccccagcc atagagcgca tccaaaatgg tcgagtcgat
      601 ccacctggcc aactggaacc agaagtccac gaagaagagc gcgaactgca ccacgctgac
      661 ggtcaccaag gtcttgaggt cataggtgcc aaccaggagc gcgagcggaa tgcagatcac
      721 caacgccatc ttcagcagcg ccagcaccat cggcagtgct tgccgcatca cgtccatcgc
      781 aggaaagaag cccagcgcac cgaccgtcat gccgacgtcg ctcgcagcgc gcgtgacgat
      841 gttcggcaac gtcatctcga tctgcccgcc gtagtcggaa tagaccgcac cctggttcaa
      901 cttctgctgc cgcggcgacg cgatcgcgcg gatcacagag tcatccacct gatcccgact
      961 caggaagccg gcccaccccg caacacgcgt cagcaagctg gggtccacct gttgcagtag
     1021 ccgcgagcgc agcccattgc cgtccgacca ccactgccgg caggttggat agccggcgcc
     1081 gctgggcacc tcggccaggc cggcatcgcg attgctgtcg tagggccaac cctcgcgggg
     1141 ggacctggag cgataggtgt cgtagaagcc cggcgtgtcc acgaagaagc gcgagccgat
     1201 ccaggtgacg tcgtgcatct gctcctcggt gagatcggga cggtccatga acagccgcgc
     1261 acgcgcaggc ccgtagcagt tgtgggtgaa gtcggccacc tcctgggcca gcaccgggtc
     1321 ggcgatgcgc gtgttgtcga tgtccatgcg gatctgtcgc aggtccgtcc cacacggaat
     1381 ggccgcgacg gatgcgcccg tcaccgcgcg cgagatcgcg tgcatgaaga accaccacac
     1441 cggcacccgc gccgactgat tgttgagcgt gctgaacgac tgcgaccagc ccgtctccga
     1501 aggctccggc acgttgacct ggcattgcgc cgagcgtgag cgatcgaact gcagcgtggt
     1561 gaaatcgacg tcgatgaacg ggatgcccgc gaacatgatg acgacgatcg cgacgaagac
     1621 gcggttctcg atacgggccg aactcagcac gcccttgtta ccttcgtccg cgccttcggc
     1681 tcgtgccttg agccattcct ggatcacgat cgccaggaag ggaatcgcga acacgccgct
     1741 ggcgaccagc acgttccaga cgccgttgtg gacaatccac gaaaccagcg tcaggtagta
     1801 ctccaggtag tcggtcgtgt agagggtcat gggggactcc ggctcatccg gcctgcatga
     1861 attggctgac ttccagcgcg acgaccgcga gcaccgcggc cacctccacg cgcagcaggc
     1921 gctggcgcgc gccgccttcc ggctcccgtg cgtgcaggcg ccggcgcatc cacagccagc
     1981 cgtagaccgt gacggcatag agcagcaacc gccagaccag gaaccagccc gcgtgcgcat
     2041 ccatccactg tcgccagccg tcgatgccgc cggccatgcg gatgccgacc aggttgacca
     2101 cggcggcggc gatcagcacc aggaccagta ccagcaacgc cagcagcgcg cgggcactgt
     2161 agcggcggac ggcgacccag ctcacgggtt gttcccgctc ggtggtcgtt gcaactcgtc
     2221 gatccggttg cgggtcggat cgccctcgta gatgccgcgc gagccggtcg agcgcgtgct
     2281 gtgccgctgg atgatggcgg tcggcgagtt gctcgccagt tcgcggcgca gttcgagctc
     2341 cgtcttcagg ttgaggatct cgcggtcaag gatgtcgctc tcgtgggaga cggccttctg
     2401 cgccagttcg ttggcagaga cgttgggctc cttgcgcccg gtcaacagcg tgcgctgcag
     2461 tagcagtgcc ttctccagca cgctcgccag cgccacctcc gacgcgaggc gcctggccag
     2521 gatgtcttgg tcgggctcgt cgcgcagcgc ttcgatcacg ccgcgcgtga tcggcaggga
     2581 cgcggcgctg gcgtcctgca gattggcgaa gctggtcggc cggctgccgg ccaccagttc
     2641 ctgtagcgct tgcagcttgg cttcgtactc ctcctggatc agcggcgtca ggccgacgcc
     2701 cggcgtggtc gtcgtcttgg tgcaggactc gcaggtgcgc tgctcctgct cgcccagcac
     2761 gcgcacggcc caggcggccg cgtcgtcggg cgagcgccag gtctcgcagg ccaggttacc
     2821 gttgcaactg ccggggtcga tgctgccggt ggcggcgacg tcgcggccgt tgagcaggtt
     2881 gtagccggcg cgggtcacgt cgccgaccac gcgcaccgg
//
LOCUS       DATCUN010000326         5857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_91451, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000326 DATCUN010000000
VERSION     DATCUN010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91451"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VIO83_12160"
     CDS             complement(<1..1080)
                     /locus_tag="VIO83_12160"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEY8332066.1"
                     /translation="MQIGIPLETHAGETRVAATPETVKKLLAQGHRVIVQSGAGIAAS
                     IPDDAYAAVGAHIGDAAAALGAELVLKVVAPSAAELELMQPGAVLVGMLNPFDSDNLA
                     RMAGHGITAFALEAAPRTSRAQSLDVLSSQANIAGYKAVLLAAHHYPRFMPMLMTAAG
                     TVKAARVLVLGAGVAGLQAIATAKRLGAVIEASDVRPAVKEQIESLGAKFVEVPFETD
                     EERECAEGVGGYARPMPASWMERQAKAVHERAKQADIVITTALIPGRKAPTLLHEATV
                     AAMKPGSVVIDLAAAQGGNCPLTVADQVVVRHGVTLVGHSNLAALVPADASALYARNL
                     LDFLKLVIDGEGKFHLNREDDIVAAC"
     gene            1398..1718
                     /locus_tag="VIO83_12165"
     CDS             1398..1718
                     /locus_tag="VIO83_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8332067.1"
                     /translation="MTVACLDLARLRANADAASSLLKTLANPDRLLLLCQLAEGERSV
                     GELQAQLGIVQPTLSQQLAVLRREGLVSTRRDGKQIHYRVVDPAVLAVIHTLYQLFCA
                     EEHP"
     gene            1715..2155
                     /locus_tag="VIO83_12170"
     CDS             1715..2155
                     /locus_tag="VIO83_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEY8332068.1"
                     /translation="MTIDWAHFTPWSALAGGILLGLATGLFVLANGRIAGISGLLGSL
                     LKPKASGWLDSLLFILGLLLAPWLWLVFAELPVIEMQVGVPGLLLAGLLVGFGARLGA
                     GCTSGHGICGLSRLSPRSLVATLCFMATGFATVFVVRHLLGAGA"
     gene            2152..2568
                     /locus_tag="VIO83_12175"
     CDS             2152..2568
                     /locus_tag="VIO83_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEY8332069.1"
                     /translation="MNRLTAFLAGLLFGIGLLLAGMADPAKVLAFLDLAGNWDPSLAL
                     VMGGGIVAALIPFTLAKRRTQSLTGCPMQLPSKREPDRALVIGSLLFGIGWGIAGICP
                     GPAVVVFGSGNLAILPFFVAMLAGMGLFRWYESRRG"
     gene            2686..3156
                     /locus_tag="VIO83_12180"
     CDS             2686..3156
                     /locus_tag="VIO83_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8332070.1"
                     /translation="MLNKLIAGTAGLLLTCTAGLAVAQVSPEDQIEYRKAGYSFMAWN
                     MGKIKAQVVEGSVAYDQQQVAAAANAIAAIANSGMGALYGPGTEKSVGKEKTRVKPEF
                     FTQKEEVAKVAIAFNQAANNLAKVAAAGDKAQIAQAFGEVGKSCKGCHDKFRMD"
     gene            complement(3415..4110)
                     /locus_tag="VIO83_12185"
     CDS             complement(3415..4110)
                     /locus_tag="VIO83_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY8332071.1"
                     /translation="MTLHIRIWDLPTRVFHWSLALSVIGALVTGWIGGNWMVWHGRLG
                     LLIAGLLAFRLAWGVLGSTYVRFGQVLRLPQSLPAYLRGDWHGGGHNPLGWLSVLAML
                     AALGFQVASGLVANDDIAFTGPLYRLVDSETSSRLTSLHRQGLWLVVALVVLHLAALL
                     FYRLVRGENLVEAMLHGKRRVSANRPIKPASGGSLPALIVALAITGGTVWAANGSWIP
                     TPPPAPAQSTPAW"
     gene            complement(4158..4937)
                     /locus_tag="VIO83_12190"
     CDS             complement(4158..4937)
                     /locus_tag="VIO83_12190"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY8332072.1"
                     /translation="MKILHLLALTLLLAGCAGGLRIDDRFQSRDQSSRVEFIVLHYTS
                     SDLPDSLKTLSQGGVSAHYLLPADQAKIYRMVDEHRRAWHAGDSQWRGRTWLNASSIG
                     IEIVNPGYTDGPEGRHWHAYPQAQIDRLIALLKDLQARHGVAPANIVGHSDIAPQRKV
                     DPGPLFPWRQLAAAGLIPWPKAAVVQDKLRQLDGRLPEVAWFQHQLERAGYAVPQHGR
                     LDQATRNVLAAFQMKYRPERHDGQPDVHSAALLLALPPAAN"
     gene            complement(5021..>5857)
                     /locus_tag="VIO83_12195"
     CDS             complement(5021..>5857)
                     /locus_tag="VIO83_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY8332073.1"
                     /translation="RAPGSRAAQHNPQASGDGGLGECLRLLSFNIQVGIGTQRYRHYL
                     TRSWQHLLPHRERAVNLQRIGALLAEFDVVALQEVDGGSLRSDYINQVEHLAQLGNFP
                     YWYQQLNRNLGRLAQHSNGVLSRLRPSLLEDHPLPGPRGRGAILLRFGEGPEALAVVM
                     MHLALGAKTRTRQLAYIRELIHGYRHCVLMGDMNTHAGELLEDSPLRDLGLVAPQTCA
                     TYPSWQPQRCIDHILLSPQLTLERMRVVDAPISDHLPVAVDIRLPAALRQQQLTELPR
                     PA"
BASE COUNT          851 a         1887 c         2145 g          974 t
ORIGIN      
        1 gcaggcggcg acgatgtcgt cttcgcggtt gaggtggaac ttgccttcgc cgtcgatgac
       61 cagcttgagg aagtccagca ggttgcgcgc gtacagcgcc gaggcatccg ccggcaccag
      121 cgcggccagg ttgctgtggc cgaccagggt cacgccgtgg cgcaccacca cctggtcggc
      181 gacagtcagc gggcagttgc cgccctgcgc cgcggccagg tcgatgacca ccgaacccgg
      241 cttcatcgcg gcgacggtgg cctcgtgcag cagggtcggc gccttgcgcc cggggatcag
      301 cgcggtggtg atgacgatgt cggcctgctt ggcgcgctcg tgcaccgcct tggcctggcg
      361 ctccatccac gacgccggca tcggccgcgc gtagccgccg acaccctcgg cgcattcgcg
      421 ttcctcgtcg gtctcgaacg gcacctcgac gaacttggcg cccagcgact cgatctgctc
      481 cttgaccgcc ggacgcacgt ccgaggcctc gatcaccgca ccgagacgct tggccgtggc
      541 gatggcctgc aggccggcga cgccggcacc aagcaccagc acgcgggcgg ccttcaccgt
      601 gccggcggcg gtcatcagca tgggcatgaa gcgcgggtag tggtgggcag cgagcagcac
      661 cgccttgtag ccggcgatgt tggcctgcga ggacagcacg tcgaggctct gcgcgcgcga
      721 ggtgcgcggc gccgcctcga gagcgaaggc ggtgatgccg tgcccggcca tacgcgccag
      781 attgtcgctg tcgaacgggt tgagcatgcc gaccagcacg gcgccgggct gcatcagttc
      841 cagttcggcg gcgctggggg cgaccacctt gagcaccagc tcggcgccca gcgcggcggc
      901 ggcgtcgccg atatgcgcgc cgacggcggc gtaggcgtcg tcgggaatgc tggcggcaat
      961 cccggcaccg ctctgcacga tgacccggtg gccctgggcg aggagtttct tgaccgtttc
     1021 cggcgtggca gccacgcggg tttccccggc gtgggtttcg agcggtatac cgatttgcat
     1081 gagcaaatct ccattggcaa acgaaattgt tgttatgtgt cgctcccgct gggcgcctga
     1141 tccactggct tggcaggccg gcggcgctgt gcgcgtgccg gcggcggggc gtaattgagg
     1201 ggcattgtgc cggggcgggc ggggcggctt caataggttg gcgtgcaatt tctacgaaat
     1261 tgcaagtcga ctttggccaa agatagtggg tttttacacc taaagtagac gtgcggctgg
     1321 accgcgcgac catcaatcta ttgaaaaata gattgtaata aggcaaattg ttcacagact
     1381 aatcgctggg agtcgccatg actgtcgcct gtctcgatct cgcgcggctg cgcgccaatg
     1441 ccgatgcggc cagcagtctg ctgaagacct tggccaatcc ggatcgcctg ttgctgctgt
     1501 gccagctggc cgagggcgag cgcagcgtcg gcgagctgca ggcgcagctg ggcatcgtcc
     1561 agccgaccct gtcgcagcag ctggcggtac tgcgccgcga ggggctggtg agcacccgcc
     1621 gcgacggcaa gcagatccac taccgtgtcg tcgatcccgc cgtcctggcg gtcattcata
     1681 ccctgtatca actgttctgc gcggaggagc atccatgacc atcgactggg cccatttcac
     1741 cccctggagc gccctcgccg gcggcatcct gctcggtctg gcgaccggcc tgttcgtcct
     1801 cgccaacggc cgcatcgccg ggatcagcgg cctgctcggc agcctgctca agccgaaggc
     1861 cagcggctgg ctggacagcc tgctgttcat cctcggcctg ttgctggcgc cctggctgtg
     1921 gctggtcttc gccgaactgc cggtgatcga gatgcaggtc ggcgtgccgg gtctgctgct
     1981 ggccggcctg ctggtcggct tcggcgcccg tctgggtgcc ggttgcacca gcggccacgg
     2041 catctgcggc ctgtcgcggc tgtcgccgcg ctcgctggtg gcgaccctgt gcttcatggc
     2101 caccggcttc gccaccgtct tcgtcgtccg ccacctgctg ggagctggag catgaatcgt
     2161 ctgactgcat ttctcgccgg gctgctgttc ggcatcggcc tgctgctggc cggcatggcc
     2221 gatccggcca aggtgctggc cttcctcgac ctggccggca actgggatcc gtcgctggcc
     2281 ttggtgatgg gcggcggcat cgtcgcggcg ctgattccct tcaccttggc caagcgccgc
     2341 acccagtcgc tgaccggctg cccgatgcag ctgccgagca agcgcgagcc ggatcgggcg
     2401 ctggtgatcg gcagcctgct gttcggcatc ggctggggca tcgccggcat ctgtccgggg
     2461 ccggcggtgg tggtgttcgg cagcggcaat ctcgccatcc tgccgttctt cgtcgccatg
     2521 ctcgccggca tgggcctgtt ccgctggtat gaaagccgcc gcggctgagt ggcgcgattg
     2581 gcccggccgg gtatgccggg cgttcgggaa agggctactc tagggcactt gccggcccag
     2641 gccggcactt ctcgtgctta gtctcaagga aagggagaac acccgatgct gaacaaactg
     2701 atcgccggca ccgccggctt gctgctgacc tgcaccgccg gcctggccgt ggcccaggtc
     2761 agccccgaag accagatcga gtaccgcaag gccggctaca gcttcatggc ctggaacatg
     2821 ggcaagatca aggcgcaggt cgtcgagggc tcggtggcct atgaccagca gcaggtggct
     2881 gccgccgcca acgccatcgc cgccatcgcc aactccggca tgggcgcgct gtacggtccg
     2941 ggcaccgaga agtcggtggg caaggagaag acccgggtga agccggagtt cttcacccag
     3001 aaggaggagg tcgccaaggt ggccatcgcc ttcaaccagg ccgccaacaa cctggccaag
     3061 gtggctgccg cgggtgacaa ggcgcagatc gcccaggcct tcggcgaagt cggcaagagc
     3121 tgcaagggtt gccacgacaa gttccgcatg gactgacagc ccatgtgaaa gctgctgcgc
     3181 tcggtcatgc tgcgttaaaa agtggctcgg acagtggctt gccactgaac gcccgacagg
     3241 gcggcccgca gggcgagtga aacgagtact gctcatttac agctcgtaaa ctccgcgtcc
     3301 tcgccacttt ttgccttgcc tgaccttcgc tcgcgacgct ttcacaaggc ctgtatggct
     3361 gtttaccttg catgaaaaag ggcgacccgc gggtcgccct ttttcgttgt ggccctacca
     3421 ggccggtgtg ctctgcgccg gtgcgggtgg cggggtcgga atccagctgc cgttggccgc
     3481 ccacacggtg ccgccggtga tcgccagggc gacgatcagc gccggcaggc tgccgccgct
     3541 ggcgggcttg atcggccggt tcgccgacac ccggcgcttg ccgtggagca tggcctcgac
     3601 caggttctcg ccgcgcacca gacggtagaa cagcagcgcg gccaggtgca gcaccaccag
     3661 cgccaccacc agccacaggc cctggcggtg caggctggtc aggcggctgc tggtctcgga
     3721 gtcgaccagg cggtacagcg gcccggtgaa ggcgatgtcg tcgttggcga ccaggccgct
     3781 ggcgacctgg aagccgaggg cggcgagcat cgccagcacc gacaaccagc ccagcgggtt
     3841 gtgcccgccg ccgtgccagt cgccgcgcag gtaggccggc aggctctgcg gcaggcgcag
     3901 cacctggccg aagcgcacgt aggtggaacc gagcacgccc caggccaggc ggaaggccag
     3961 caggccggcg atcagcaggc cgaggcggcc gtgccagacc atccagttgc cgccgatcca
     4021 gccggtgacc agggcgccaa tgacgctgag cgccagcgac cagtggaaga cgcgggtggg
     4081 caggtcccag attcgtatgt gcagggtcat cggcactcct tgcgcggcgg gtggtgggca
     4141 gtctggctgc ggtccgttca gttggccgct ggcggcaggg ccagcagcag ggcggcgctg
     4201 tgcacgtcgg gctggccgtc gtggcgctcg gggcggtact tcatctggaa ggcggcgagc
     4261 acgttgcgcg tggcctggtc gagccggccg tgctgcggta ccgcatagcc ggcgcgttcg
     4321 agctggtgct ggaaccaggc gacctccggc agccggccgt cgagctggcg cagcttgtcc
     4381 tgcaccacgg cggccttggg ccaggggatc agtccggctg cggcgagctg gcgccagggg
     4441 aacagcgggc cggggtcgac cttgcgctgc ggggcgatgt cgctgtggcc gacgatgttg
     4501 gccggcgcca cgccgtggcg ggcctggagg tccttgagca gggcgatcag gcggtcgatc
     4561 tgcgcctgcg ggtaggcatg ccagtggcgc ccctcggggc cgtcggtgta gccgggattg
     4621 acgatctcga tgccgatcga gctggcgttc agccaggtgc gcccgcgcca ctggctgtcg
     4681 ccggcgtgcc aggcgcgacg gtgctcgtcg accatgcggt agatcttcgc ctggtcggcc
     4741 ggcagcaggt agtgggcgct gacgccgccc tggctgaggg tcttcagcga gtcgggcagg
     4801 tcgctggagg tgtagtggag gacgatgaat tcgacccggc tgctctggtc gcgcgactgg
     4861 aaacggtcgt cgatgcgcag gccgccggcg cagccggcga gcagcagggt gagggcaagc
     4921 aggtggagga tcttcatggc tgtgcggcgg cggatctggt gcggatggtc agcatacgcg
     4981 gttacgcggt gcgcggcgac cgcacgccag tcggcggcgc tcaggccggg cgcggcagct
     5041 cggtcagctg ctgctgacgc agggcggcgg gcaggcggat atccaccgcc accggcaggt
     5101 ggtcggagat cggcgcatcc actacgcgca tgcgctcgag ggtcagctgc gggctgagca
     5161 ggatgtggtc gatgcaacgc tgtggctgcc agctggggta ggtggcgcag gtctgcgggg
     5221 cgaccaggcc gagatcgcgc agcggcgagt cttccagcag ttcgccggca tgggtgttca
     5281 tgtcgcccat cagcacgcag tggcggtagc cgtggatcag ttcgcggatg taggccagct
     5341 ggcgggtgcg cgtcttggcg ccgagggcca ggtgcatcat caccaccgcc agcgcctccg
     5401 ggccttcgcc gaaacgcagc aggatggcgc cgcggccgcg cgggccgggc agcgggtggt
     5461 cttccagcag gctcgggcgc agacggctga gcacgccgtt gctgtgctgg gccaggcggc
     5521 cgaggttgcg gttgagctgc tggtaccagt aggggaagtt gccgagctgg gccagatgct
     5581 cgacctggtt gatgtagtcg gaacgcaggc tgccgccatc gacttcctgc agggcgacca
     5641 catcgaactc ggcgagcagg gcgccgatgc gctgcaggtt caccgcgcgc tcgcggtgcg
     5701 gcagcagatg ctgccagctg cgggtcaggt agtggcggta gcgctgggtg ccgatgccga
     5761 cctggatgtt gaagctgagc aggcgcaggc actcgccgag gccgccgtcg ccggaggcct
     5821 gcgggttgtg ctgcgcggcc cggctgccgg gcgcgcg
//
LOCUS       DATCUN010000327         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_167848, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000327 DATCUN010000000
VERSION     DATCUN010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167848"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VIO83_12200"
     CDS             <1..459
                     /locus_tag="VIO83_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008047633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8332074.1"
                     /translation="LMLRMDKNIAATQDWLGTEKPMFFSVQLFDKWVQNYDRDDALLY
                     SVGWGGQVQEHSVLATTIFDLSYNSGRIRPNLVLGADLSNGGGFAVPSVTFELSSKVR
                     WKVEYDAFWGSDARDGDECGFPNAASCDNATLFDYFNNRDQLYTSVTYLF"
     gene            480..>1619
                     /locus_tag="VIO83_12205"
     CDS             480..>1619
                     /locus_tag="VIO83_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008047631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HEY8332075.1"
                     /translation="MNALRFPLRHTLTVGLLAAAGSLSALAVAAELPAGTVISAANID
                     QVKNDTFDGHSIASLLTEKMEWRIRNSGWKLPLAKSQEVPLDPRWVKASQANVGKATI
                     NKEACRVDNWGAGAPFPNIDMKDPQAAEKLVWNWHLGQLVGDVSQVPQYTQLLIDGKK
                     GVHAEPVAEFSRYTMKGRLTSEQTVEGDGSERGRQLLYFKSPSDMKGLGTYTQMYDSD
                     KVNSVWAYIPAVRRVRQLSGGAWMDPVGSSDQLQDDLEIFNARPCWYPEYKLLGKRWV
                     LAVANSKTGLWNPEGKSFAEKYPVVENAAPYWNMNNNNFEPREVYVVEAITPSAHPYS
                     KKVLYIDAKYPRFYYAEAYDRKGEFWKFMEFHSYSNTADGDIRTTA"
BASE COUNT          316 a          563 c          500 g          240 t
ORIGIN      
        1 ctgatgctgc gcatggacaa gaacatcgcc gccacccagg actggctggg taccgagaag
       61 ccgatgttct tctcggtgca gctgttcgac aagtgggtgc agaactacga cagggacgac
      121 gcgctgctct acagcgtcgg ctggggcggc caggtccagg agcactcggt gctcgccacc
      181 accatcttcg acctcagcta caacagcggg cgcatccgcc cgaacctggt gctcggcgcc
      241 gacctcagca acggcggcgg cttcgccgtg ccctcggtca ccttcgagct gtccagcaag
      301 gtgcgctgga aggtcgagta cgacgccttc tggggcagcg acgcgcgcga cggcgacgag
      361 tgcggcttcc cgaatgcggc ctcctgcgac aacgccacgc tgttcgacta cttcaacaac
      421 cgcgatcagc tctacaccag tgtgacctac ctgttctgat ccccatcgga gagaccccca
      481 tgaacgcact ccgctttccc ctgcgccaca ccctgacggt cggtctcctc gcggcggccg
      541 gcagcctgtc ggccctcgcc gtcgccgccg aactgccggc gggcacggtg atttccgccg
      601 ccaacatcga ccaggtcaag aacgacacct tcgacggcca cagcatcgcc agcctgctga
      661 ccgagaagat ggaatggcgc atccgcaaca gcggctggaa gctgccgctg gccaagtccc
      721 aggaagtgcc gctcgacccg cgctgggtca aggcctcgca ggccaacgtg ggcaaggcca
      781 ccatcaacaa ggaagcctgc cgggtggaca actggggcgc cggcgccccc ttcccgaaca
      841 tcgacatgaa ggacccgcag gccgccgaga aactggtgtg gaactggcac ctcggccagc
      901 tggtcggcga cgtctcccag gtgccgcagt acacccagct gctgatcgac ggcaagaagg
      961 gcgtgcacgc cgagccggtc gccgagttca gccgctacac catgaagggc cgcctgacca
     1021 gcgagcagac ggtcgagggt gacggcagcg agcgcggccg ccagttgctg tacttcaagt
     1081 cgccctccga catgaagggt ctcggcacct acacccagat gtacgactcg gacaaggtca
     1141 acagcgtgtg ggcctacatt cccgccgtgc gccgggtgcg ccagctgtcc ggcggcgcct
     1201 ggatggaccc ggtcggctcc tccgaccagc tgcaggacga cctggagatc ttcaacgccc
     1261 ggccgtgctg gtaccccgag tacaagctgc tcggcaagcg ctgggtgctc gccgtggcca
     1321 acagcaagac cggcctgtgg aatccggagg gcaagagctt cgccgagaag tacccggtgg
     1381 tggagaacgc ggcgccctac tggaacatga acaacaacaa cttcgagccg cgtgaggtct
     1441 atgtggtcga ggcgatcacc ccgtcggcgc acccgtacag caagaaggtg ctgtacatcg
     1501 acgcgaagta cccgcgcttc tactacgccg aggcctacga ccgcaaaggc gagttctgga
     1561 agttcatgga gttccactcc tactccaaca ccgccgacgg cgacatccgc accaccgcc
//
LOCUS       DATCUN010000328         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_17248, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000328 DATCUN010000000
VERSION     DATCUN010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17248"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2695
                     /locus_tag="VIO83_12210"
     CDS             <1..>2695
                     /locus_tag="VIO83_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEY8332076.1"
                     /translation="LDEAMQPLPIGSIGELYIGGVGVARGYLKRPDLTEERFLASPFV
                     AGDRLYRTGDRCRFLADGNIEYLGRLDHQVKLRGQRIELGEIDAALLAQAGVRDAATL
                     LLDQRLVSFWCGDADEATLRAELSDSLPAHMQPSLFVQLDSLPLNSNGKLDRKALAAT
                     PLPQSQSDQPRTAPRNATESVLCELFGEVLGCSPVGIEDHFFQLGGHSLLATRLVSRV
                     RERLGVSLPLPLVFAQPTVAALAEHLRAAMPGESISAQPRPAQLPLGLAQRRFWMLSR
                     LVPESREYHMPFALTLRGELQVQVLREAFEQLSQRHLVLRSRIVEVDGEPQLLIDDHG
                     PALAVTDVATQDWASACANAQDELMTAFDLAAAAPWRAHLLQRQGSDENLLLVCLHHS
                     ATDGWAMQLLIDELTQAYAAGLNGQSPAWTPLEIDYVDFALWQQHPDTQARRSDSLDY
                     WKNHLGQEDYQLDLPIDHPRGAEANRRARQLTLRLGTQRAEAIRQFARQRGTTTYVVL
                     ATALSALLARYSGQREIRFGTPADQRDQPQAQTLLACLVNTLVIRSDVDQRAPAEQLL
                     TSLEADLRAAQAHADLPFATLVGAVSQNRDLSRTPLFQVLFSLNYGRVDSSQWPGLNV
                     EEQMLPVIDAKFEQSWEVQDDGSDMTLALEYQSALFDETTMQRWSAQWCALLDALVAT
                     PGRTLVELFPQQQDQQQLDRWNATERHYSGPTNLHTALSQQAALSPNAPALVFEGQRL
                     SYAELDQRAQTVARALRAAGVGTDSIVPLCMERSVEMVVALLGIVHAGAAWLPLDPEL
                     PAARLAFLIEDADAKVTLTQAQWLSRLPTGHQAWTLDALPQAPAFEPISVEAHDLAYV
                     LYTSGSTGQPKGVMNEHGALMNRLHWMQDAFPIGPNDRVLQK"
BASE COUNT          478 a          944 c          826 g          447 t
ORIGIN      
        1 cctcgacgag gccatgcaac cgttgccgat tggcagcatc ggcgagctgt acatcggtgg
       61 cgtcggcgtg gcccgtggtt acttgaaacg tccggacctg accgaggaac gcttcctcgc
      121 cagtccattc gtggccggtg accgcctgta ccgcacgggc gaccgttgcc gcttcctggc
      181 cgatggcaac atcgagtacc tggggcgtct cgaccatcag gtgaagttgc gcggccagcg
      241 catcgagctg ggggaaatcg acgccgcact gctcgcgcag gccggtgttc gcgatgccgc
      301 taccttgttg ctcgaccagc gcctggtgag tttctggtgc ggagacgccg atgaggcgac
      361 cctgcgtgcc gagttgagcg acagcctgcc cgcgcacatg cagcccagcc tgttcgtgca
      421 gctcgacagc ctgccgctca acagcaacgg caagctcgac cgcaaggcat tggccgccac
      481 gccgctgcca cagagccaaa gcgaccagcc gcgcacagca ccgcgcaacg ccactgaaag
      541 cgtactctgc gaactgttcg gcgaagtgct gggttgttcg ccagtcggca tcgaagatca
      601 cttcttccaa ctcggcggcc actccttgct ggccacccgc ctggtcagtc gggtccgtga
      661 gcgccttggg gtttccctgc ccttgccgct ggtgttcgcc caaccgacgg tcgcggccct
      721 ggccgagcat ctgcgcgcgg ccatgcccgg tgagtcgatc agcgcacaac cgcgcccggc
      781 gcaattgccc ctgggtctgg cccagcgccg attctggatg ctcagtcgcc tggtgccgga
      841 atcccgcgag taccacatgc cgtttgccct gaccttgcgc ggtgagctgc aggtccaggt
      901 cttgcgcgag gcctttgagc agctcagcca gcgccatctg gtgctgcgca gccggattgt
      961 cgaagtcgac ggcgagccgc aattgctgat cgacgaccac ggaccggcgc tcgcggtcac
     1021 tgacgtcgcg acccaggact gggcctcggc ctgcgccaac gcccaggacg aactgatgac
     1081 agcgttcgac ctggccgccg ccgcaccttg gcgcgcgcat ctgctgcaac gtcagggcag
     1141 cgacgaaaac ctgctgctgg tgtgcctgca ccacagtgcc accgacggtt gggccatgca
     1201 gctgctcatc gacgaactga cccaggccta cgccgccggc ctcaacgggc agtcgccggc
     1261 ctggacgccg ctggagatcg actacgtcga tttcgccctg tggcaacaac acccggacac
     1321 ccaggcccga cgcagtgaca gcctggacta ttggaaaaac cacctgggcc aggaagacta
     1381 ccaactcgac ctgcccatcg accatccgcg tggcgccgaa gcaaatcgtc gcgcccggca
     1441 actgaccctc cgtctcggca cccagcgcgc cgaagccatc cgtcagttcg cccgtcagcg
     1501 cggcaccacc acctatgtgg tgctcgccac ggccttgagc gcgctgctgg cgcgttacag
     1561 cggtcaacgg gagattcgct tcggcacccc ggcggaccag cgtgatcaac cgcaagccca
     1621 gacactgctg gcctgtctgg tcaacaccct ggtgatccgc agcgacgtcg accaacgggc
     1681 tccggccgag caattgctca ccagccttga ggccgacctg cgcgcagccc aggcccatgc
     1741 cgacctaccc ttcgccaccc tggtcggtgc tgtctcgcaa aaccgcgacc tgagccgtac
     1801 accgctgttc caggtgctgt tctccctcaa ctatggccgg gtggacagca gccaatggcc
     1861 ggggctgaac gtcgaagagc agatgctgcc ggtgatcgac gccaagttcg aacagagctg
     1921 ggaagtccag gacgatggca gcgacatgac gctggcgctg gaataccaat cggccttgtt
     1981 cgacgaaacg acgatgcaac gctggagcgc gcagtggtgt gcgctgcttg atgccttggt
     2041 ggcaacccct gggcgcacgc tggtcgaact gttcccgcaa cagcaggatc agcagcaact
     2101 ggatcgctgg aacgccaccg aacgtcacta cagcggcccg accaacctgc acaccgcgct
     2161 cagccaacaa gccgccctga gccccaacgc cccggcattg gtgttcgaag gtcagcgcct
     2221 gagctatgcc gaactggatc aacgggcgca aaccgttgcc cgggccctgc gcgccgccgg
     2281 cgtgggcact gacagcatcg tgccgctgtg catggagcgt tcggtggaga tggtcgtggc
     2341 cttgctcggt atcgtccatg ccggtgccgc gtggttgccg ctggacccgg aattgccggc
     2401 tgcacgcctg gcgttcctga ttgaggatgc cgatgccaag gtcaccttga cccaggcgca
     2461 gtggctgtcg cgcctgccaa ccggacatca agcctggacc ctcgacgctc tgccccaggc
     2521 tccagcgttc gagccgatca gcgttgaagc tcacgacctg gcctacgtgc tctacacctc
     2581 cggttccacc ggccaaccca aaggcgtgat gaacgaacac ggcgccttga tgaaccgcct
     2641 gcactggatg caggacgcct tcccgattgg cccgaacgac cgtgttctgc aaaag
//
LOCUS       DATCUN010000329         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_205412, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000329 DATCUN010000000
VERSION     DATCUN010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205412"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..437)
                     /locus_tag="VIO83_12215"
                     /pseudo
     CDS             complement(1..437)
                     /locus_tag="VIO83_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611802.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(557..1785)
                     /locus_tag="VIO83_12220"
     CDS             complement(join(557..1506,1506..1785))
                     /locus_tag="VIO83_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEY8332077.1"
                     /translation="MRKSTKFSPELRERAIRLVGEARGQHESQWAAVVSIAGKVGCTP
                     QTLLTWVRQHERDTGQRRGVTTAEQDRVKALERENRELRRANEILKLASGFFRPGGAR
                     PPHQAMRAFVDAHRGCFGVEPICKVLQIAPSGYRRHAARRKEPALSPARAQRDALLMP
                     KIEQVWRINMRVYGADKVWRQLRREGTSVARCTVERLMRQQGLRGVLRGKVVRTTTPD
                     TSAPCPLDRVQRAFRADRPNQLWVSDFTYVSTWQGFVYVAFVIDVFARRIVGWRVSAS
                     MQTDFVLDALEQALYARRRERGEGLVHHSDRGSQYVSIRYSERLAEAGIEPSVGSTGD
                     SYDNALAETINGLYKAELIHRRAPWKSRDAVELATLEWVSWFNHRRLLSSIGYVPPAE
                     AEAAYYQQLQPEQAIPA"
     misc_feature    complement(1391..1507)
                     /locus_tag="VIO83_12220"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          330 a          677 c          614 g          354 t
ORIGIN      
        1 cttcggggcg gcgatcagcg tcgcgtccac gagggtgccc tcgcgcagca gcaggccctg
       61 ccgggccagg tgcgcgttga tcgtctcgaa gatcacgccg gtcagaccct gatcctccag
      121 caggcggcgg aacttcagca gtgtggtcgc atccggcgcc gcctcgcggg ccaggtcgat
      181 gccgatgaag cggcggatgg cgtggctgtc gtacacggcg tcctcgatac cctcgtccga
      241 cagcccgaag cactgctgcg ccacatacat gcgcagcatg cgcgagagcc ccagcggcgg
      301 ccggccgggt agaacggcgc cagcgccgcc tccagctgcg cccatggcgt cagcgtctcc
      361 agctgcgcca ggaagcgctc gcggcgcgtc tgcttcttct tgccggcgta ttcgaggtcg
      421 gaaaagctcg tttgcatgac gtctgctcac cacggctgcg gaccaaggcc gcattgtctc
      481 aggagcacgg cctcacgcca ccagccggcg ggaataatga accgccccgg gaatcgtaga
      541 ggcccgttgg tttgagtcag gccgggatgg cctgctcggg ttgcagttgc tgatagtagg
      601 cggcttcagc ctcggccggt ggaacataac cgatggagct gagcaggcgg cggtggttga
      661 accaggacac ccattccagg gtagccagct cgaccgcgtc gcgggatttc cacggggccc
      721 ggcggtggat cagttccgcc ttgtagaggc cgttgatggt ctccgccagc gcgttgtcgt
      781 agctgtcccc cgtgctgccc acggagggct cgatgccggc ctcggcgagg cgctcgctgt
      841 aacggatcga aacgtactgc gacccgcgat cgctgtggtg gaccagcccc tcgccgcgtt
      901 ccctgcggcg ggcgtagagc gcctgctcca aggcatcgag gacgaaatcg gtctgcatcg
      961 aagcgctgac ccgccagccg acgatgcgac gggcaaagac gtcgatcacg aacgccacat
     1021 agacgaaccc ctgccaggtc gatacatagg tgaagtccga gacccagagc tggttggggc
     1081 gatcggccct gaaggcccgc tggacgcggt ccaggggaca gggggctgag gtatccggtg
     1141 tcgttgtcct cacaaccttg ccccgtagca cgccgcgcag cccctgctgg cgcatcagcc
     1201 gctccaccgt gcagcgggca acggatgtcc cctcacggcg cagctggcgc cagaccttgt
     1261 cggcgccgta gacccgcatg ttgatgcgcc agacctgctc gatctttggc atcagcaggg
     1321 catcgcgctg tgcccgcgca ggtgacaacg caggctcttt ccggcgggcc gcatgacgcc
     1381 gatagccgga tggggcgatc tgcagcacct tgcagatcgg ctcgaccccg aaacagcccc
     1441 ggtgggcatc cacaaacgcc ctcatcgctt ggtgtggcgg tcgagctccg cctgggcgaa
     1501 aaaagcactc gccagcttca ggatctcgtt ggcccggcgc agctcgcgat tctcgcgctc
     1561 cagcgccttc acccgatcct gctcagccgt cgtcacccca cgccgctgac cggtatcccg
     1621 ctcgtgctgc cgtacccagg tcagcagggt ttgcggcgtg cagcccacct tgcccgcgat
     1681 cgacaccacc gccgcccatt gcgactcgtg ctgcccacgt gcctctccca ccaaccggat
     1741 cgctcgctca cgcagctccg gtgaaaactt cgtcgacttc ctcatggctc cattctctca
     1801 agtgatggag cctccacaaa agccggggcg gttcaataaa tcagcgtttc cttagctact
     1861 taaatatatc ggacttctta ttttttgttt tattccatac tttcttaatg gttaacacct
     1921 acctggtcga tgtcctgcag cgcgtcggcc agccacccgg ccgcacgcgt cgccg
//
LOCUS       DATCUN010000330         4414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_17342, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000330 DATCUN010000000
VERSION     DATCUN010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17342"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VIO83_12225"
     CDS             <1..972
                     /locus_tag="VIO83_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011333321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8332078.1"
                     /translation="ASGQIQKIHVEVGDVVKEGQLLVEIDPSTQQAKLDAGRFSIENL
                     KAQLQEQRAQHDLAQQKFQRQQHLKAGNATREEDVQTAQAELRATQARIDMIQAQIRQ
                     AQASLRSDQAELGYTRIYAPMSGTVVALDAREGQTLNAQQQTPLILRIAKLSPMTVWA
                     EVSEADIGHVKPGMNAWFTTLSGGTRRWKSTVRQILPVPPKPLDQTSQGGGSPASSSK
                     SGSARVVLYTVLLDVDNADNALMAEMTTQVFFVAGQAQNVLTAPTAALQAGAQSDSQT
                     AQVVASNGRIEQRNVRTGISDRLRVQILDGLQEGDHLLIGPADGSGG"
     gene            973..2946
                     /locus_tag="VIO83_12230"
     CDS             973..2946
                     /locus_tag="VIO83_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEY8332079.1"
                     /translation="MHTPLIELSDIRKAYGGGDAPEVHVLRGIDLSIHAGEFVAIVGA
                     SGSGKSTLMNILGCLDRPTSGEYRFAGENVADLDSDELAWLRREAFGFVFQGYHLIPS
                     GSAQENVEMPAIYAGTPAAERHARAAALLERLGLATRTGNRPHQLSGGQQQRVSIARA
                     LMNGGHIILADEPTGALDSHSGKEVMALLDELASQGHVVILITHDREVAARAKRIIEI
                     RDGLIISDSALDNRAAQTPANPGALQAVDLRKRLTEGTQASGAWKGELVDAVQAAWRV
                     MWINRFRTALTLLGIIIGVASVVVMLAVGEGSKRQVMAQMGAFGSNIIYLSGASPNPR
                     TPLGIVTLDEVKAVASLPQVMRIMPVNGQEAGVRFGNLDHLSYVGGNDTNFPAIFNWP
                     VVQGSYFTEADERNAAAVAVIGHKVRTKLLKDVANPIGQYILIENVPFQVVGVLAEKG
                     ASSGDSDSDDRIAIPYSAASVRLFGTYNPEYIAIAAADARKVKETEHAIEQLMLRLHN
                     GKKDFELTNNAAMIQAEARTQNTLSLMLGSIAAISLLVGGIGVMNIMLMTVRERTREI
                     GIRMATGARQRDILRQFLTEAVMLSVVGGLAGIALALIVGGVLILSEVAVAFSLIAVL
                     GAFGCALVTGVVFGFMPARKAARLDPVTALTSE"
     gene            2954..4348
                     /locus_tag="VIO83_12235"
     CDS             2954..4348
                     /locus_tag="VIO83_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY8332080.1"
                     /translation="MKPQLSLLTLCLLLGACGSPAQRPDSGITPPTAWQSPHSSTATR
                     DNQQWWNRFGSPQLGRLIEQARVGSYDLAAAIARVRQAQAETVVAGGSQLPEVQAGAN
                     ANRQKLLRGNGYSQLDADSDNSAVDYFNANLTATYEIDFWGGQRASRDSAQFALQASE
                     FDQATVELTLLSGVANTYALTLSLQEQSRIAGLNLANAQNVLKLVQTRFDSGSATALE
                     LAQQKSLVAAQQRQLPLVRQQAIDAQISLATLLGRPVQELALGREHFDQLSWPAIDAG
                     VPSELLSRRPDIARAEAQLAAAQADVTVARAAMLPTLTLSAEIGSGADRAGDILRSPF
                     YNLTAGLLAPVFNNGRLGAERDQATARQEELLEAYRGAIINGFADVEKALNSIRGLDA
                     QRQWQSEELNQAQTAFNIAQSRYEAGAEDLLTVLETQRTLYAAQDLNVQLRLSRMQAS
                     IALYKALGGGWQVL"
BASE COUNT          874 a         1488 c         1355 g          697 t
ORIGIN      
        1 gcgtccgggc agattcaaaa gatccacgtg gaagtcggtg acgtggtcaa ggaaggccag
       61 ctgctggtgg aaatcgatcc ctccacgcaa caggccaaac tcgatgccgg gcgtttttcc
      121 atcgaaaacc tcaaggcgca gcttcaggag caacgcgcgc aacacgacct ggcccaacag
      181 aaattccagc gccagcaaca cctcaaggcc ggcaacgcca cccgcgaaga agacgtgcag
      241 actgcgcagg ccgaactgcg cgccacccag gcgcgcatcg acatgatcca ggcgcagatt
      301 cgccaggccc aggccagcct gcgcagcgat caggccgagc tcggctacac ccggatctat
      361 gcgcccatgt ccggcaccgt ggtcgcactg gatgcccgcg agggccagac cctcaacgcc
      421 cagcaacaga caccgctgat tctgcgcatc gccaaactgt cgccgatgac cgtctgggcc
      481 gaggtctccg aagccgacat cggccacgtc aaacccggca tgaacgcctg gttcaccacc
      541 ctgagcggcg gcacccggcg ctggaaaagc accgtgcggc agatcctgcc cgtaccgccc
      601 aaacccctag accaaaccag ccagggcggc ggcagccccg ccagctcgag caaaagcggc
      661 agcgcacggg tggtgctgta caccgtgctg ctggatgtcg ataacgccga caacgcgctg
      721 atggcggaaa tgaccactca ggtatttttc gtcgcaggcc aggcgcagaa cgtcctgacc
      781 gcaccgaccg ccgcgctgca ggccggcgcg caatccgaca gccagaccgc gcaagtggtg
      841 gccagcaatg gccgtatcga acaacgcaac gtgcgcaccg gtatcagcga ccgcctgcgg
      901 gtgcagatcc tcgacggcct gcaggaaggc gatcacttgt tgatcggccc ggccgacggg
      961 agtggcggtt gaatgcacac gcccctgatc gagctttcag acatccgcaa agcctacggc
     1021 ggtggcgacg cgcctgaagt ccatgtgttg cgcggcatcg acctgtcgat ccatgccggg
     1081 gaattcgtgg cgatcgtcgg cgcctccggc tccggcaagt cgacactgat gaacatcctc
     1141 ggctgcctcg accgtccgac ctcgggcgaa taccgcttcg ccggagaaaa cgtcgccgac
     1201 ctggacagcg acgaactggc ctggctgcgc cgcgaagcct ttggtttcgt gttccagggt
     1261 tatcacctga ttccgtccgg ttcggcccag gagaacgtcg agatgccggc gatctacgct
     1321 ggcactccag ccgccgaacg acatgcccgc gccgccgccc tgcttgagcg cctcggcctg
     1381 gccacacgca ccggcaatcg tccacaccag ctctccggtg gccagcagca acgggtgtcc
     1441 attgcccgcg ccttgatgaa cggcggccat atcatcctcg ccgacgaacc caccggcgcc
     1501 ctcgacagtc acagcggcaa ggaagtcatg gcgctgctcg acgagctggc gagccagggc
     1561 cacgtggtga tcctcattac ccatgatcgc gaagtggcgg cgcgggccaa gcgcatcatc
     1621 gagatccgcg acggcctgat catcagtgac agcgccctgg acaatcgcgc cgcgcaaacc
     1681 cctgccaatc ccggtgcatt gcaagcagtg gacctgcgca agcgcctgac tgaaggcacg
     1741 caagccagcg gtgcctggaa aggcgagctg gtcgacgcgg tgcaagccgc ctggcgggtg
     1801 atgtggatca acaggttccg caccgcgctg accctgctgg ggatcattat cggcgtggcg
     1861 tcggtggtgg tgatgctggc cgtcggcgaa ggcagcaagc gccaagtcat ggcccagatg
     1921 ggcgcgttcg gttccaacat catttacctc agtggcgcgt ccccaaaccc gcgcacgccg
     1981 ctgggtatcg tcaccctgga cgaagtcaag gcggtggcga gcctgcccca ggtgatgcgc
     2041 atcatgccgg tcaacggcca ggaagccggg gtacgcttcg gcaacctcga ccacctgagc
     2101 tacgttggcg gcaacgacac caacttcccg gcgatcttca actggccagt ggtgcaaggt
     2161 agctacttca ccgaggccga tgaacgaaac gccgcggcgg tcgcggtgat cggccacaag
     2221 gtgcggacca aactgctcaa ggacgtcgcc aatcccatcg gccagtacat cctgatcgaa
     2281 aacgtaccct tccaggtggt cggcgtgctg gcggaaaaag gcgccagctc cggcgactcc
     2341 gacagcgacg accgcatcgc catcccctac tccgccgcca gcgtgcggtt attcggcact
     2401 tacaacccgg aatacatcgc gattgccgcc gccgatgcgc gcaaggtcaa ggaaacggaa
     2461 cacgccatcg agcaattgat gctgcgcctg cacaacggca agaaggattt cgagctgacc
     2521 aacaacgcgg cgatgatcca ggccgaagcg cgcacgcaaa acaccctgtc gctgatgctc
     2581 ggttcgattg ccgcgatttc gctgctggtg ggcggcatcg gcgtgatgaa catcatgctc
     2641 atgaccgtgc gcgaacggac ccgcgagatc ggcatccgca tggccaccgg tgcccgccag
     2701 cgcgacatcc tgcgccagtt cctcaccgaa gcggtgatgc tttcggtggt cggtggcctg
     2761 gccgggatcg ccctggcgct gatcgtcggc ggcgtgctga tcctcagcga agtcgccgtc
     2821 gcgttctcct tgatagcggt gttgggcgcc ttcggctgcg ccctggtcac cggtgtcgtc
     2881 ttcggcttca tgccggcccg caaagctgcc cgactcgacc cggtcacggc ccttaccagt
     2941 gaatgatctt tccatgaagc cgcaactgag tttgctgacc ctctgcctgc tgctcggcgc
     3001 ctgcggcagt cccgcccagc gtccggacag cggcattacg ccgcccaccg cctggcaatc
     3061 accacactcc tcgactgcta cccgcgacaa ccagcaatgg tggaaccgct ttggcagccc
     3121 gcaactggga cgcctgatcg aacaggcccg agtcggcagc tacgacctgg ccgccgccat
     3181 tgcgcgggtg cgccaggcac aggccgaaac ggtggtcgcc ggtggttcgc agttaccgga
     3241 agtccaggca ggggcaaacg ccaaccgcca gaaattgctg cgcggcaatg gctacagcca
     3301 actggatgcc gacagcgaca acagcgccgt ggactatttc aacgccaacc tgaccgccac
     3361 ttacgaaatc gatttctggg gtggccagcg cgcttcccgt gacagtgcgc aattcgcgtt
     3421 gcaggccagc gagttcgatc aggcgacggt ggagctgacg ttgctcagcg gcgtcgccaa
     3481 cacctatgcg ctaaccctgt cgctgcagga gcaaagccgt attgccgggc tcaacctggc
     3541 caacgcgcag aacgtgttga aactggtgca gactcgcttc gattccggct ccgcgaccgc
     3601 actggaactg gctcagcaga aaagcctggt ggccgcgcaa caacggcaat taccgctggt
     3661 acggcagcag gccatcgacg cgcagatcag cctcgctacc ctcctcggcc gcccggtgca
     3721 ggaactggcg ctgggccggg aacatttcga tcaattgtcc tggcccgcca ttgacgcggg
     3781 cgtacccagc gaattgctca gccggcgccc ggacatcgcc cgggccgaag cgcaactggc
     3841 cgctgcccag gccgacgtca ccgtcgcccg cgccgcaatg ttgccgaccc tgaccctgag
     3901 cgcggaaatc ggctccggtg ccgaccgtgc cggtgacatc ctgcgcagcc ccttctacaa
     3961 cctcaccgcc ggtttactcg ccccggtgtt caacaacggg cgcctgggcg ccgagcgcga
     4021 ccaggccaca gcgcgccagg aggaactgct ggaagcctat cgtggtgcga tcatcaacgg
     4081 tttcgccgac gtggaaaaag ccctcaacag tattcgtggg ctggacgcac aacggcagtg
     4141 gcaaagcgag gaactgaacc aggcgcagac cgcgttcaac atcgcacaaa gccgctatga
     4201 agcgggtgcc gaggatttac tgacagtgct ggagacgcaa cgcacactgt atgcggcgca
     4261 ggatttgaat gtgcaactga ggctgtcgcg gatgcaggcg agcattgctt tgtacaaggc
     4321 gcttgggggt gggtggcagg ttttgtagtt gatcgttccc acgctctgcg tgggaatgca
     4381 gcccgggacg ctctgcgtcc catccagagc cgaa
//
LOCUS       DATCUN010000331        13237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_92039, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000331 DATCUN010000000
VERSION     DATCUN010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 13237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13237
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92039"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1848)
                     /locus_tag="VIO83_12240"
     CDS             complement(82..1848)
                     /locus_tag="VIO83_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017847301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEY8332081.1"
                     /translation="MIRRLIPLFALLLLAGLAPVLLPTFHVTLLNYIGLYSLVALGLV
                     LLTGIGGMTSFGQAAFVGIGAYASAVLTTQYGLSPWLGLLAGLAITLGVATSLGLLTL
                     RLAGHYLPIGTLAWGLALFYLFGNLPGLGGFGGLQNLPNISLFGWQVRDPADFSLLIG
                     LLLAISLWLLDNLLHSRQGRAIRTLKDSAGMAEAMGIDTARSRLQVFIIAALLAALSG
                     WLYAHLQRVVNPTPFSVVMGIEYLFMIVLGGVASLWGALLGAALLTLIKQVLQDVLPQ
                     LFGHADNVEMIFFGVLVILVLQYARGGLLPLLSGLFPTAQPRPLPEHPAHALPRRTMP
                     ANGQPLMVAEGLLKQFGGLVANKDMGLTVNGAEITALIGPNGAGKSTLFNLLSGVLAP
                     NAGQIVFLGQRIDGLSPRAIARLGVSRTFQHVRLLPDMSVLENVALGAHARGKAGLLR
                     SMLHLEREEEAALLAEARRQIERVGLGAALHTPAGSLPLGQQRIVEIARALCSDPLLL
                     LLDEPAAGLRHGEKQALAHLLEQLRSEGLGVLLVEHDMEFVMGLADQIVVMEFGQRLA
                     GGTPLQIQQNPAVLAAYLGGVE"
     gene            complement(1848..2885)
                     /locus_tag="VIO83_12245"
     CDS             complement(1848..2885)
                     /locus_tag="VIO83_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017847302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8332082.1"
                     /translation="MDISILAFLAQDGLTTGAIYALVALALVLVFSVTRTILVCQGDF
                     VTYSALTLATLQLGLVPGTLWLLLGCSLAACTLDSIAALRRGQSRRVPGLLARYLGLP
                     TVLWVVLDTFELAGLPAVLQILLTLALVTPLGPLLYRLVYQRVAETSVLLLLIVSVAV
                     HITLVGISLLMFGAEGVRTDALAEAVFMLGELPVGLQSLLVIASALLLIGLLYAFFGH
                     SLYGKALRATALNRKGAQLVGIPTQLAGKAAFGLAALIGAISGVLIGPLTTLYYDSGF
                     LISLKGFVAAIFGGLASYPIAAASAFFVGVLESFANFGASAYKEVIVFTLVIPVLLWL
                     SLKHPHLEEQH"
     gene            complement(3342..3923)
                     /locus_tag="VIO83_12250"
     CDS             complement(3342..3923)
                     /locus_tag="VIO83_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017847303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8332083.1"
                     /translation="MSSRTNLTREDWIHAAQEVLVTSGVDAVRVDTLAKELKITRGSF
                     YYHFKSRGELLEGILGNWRARATEDVILHLRNAHSSPLQQLHRLLELPSHGQTAKDAA
                     SIELGIRGWARRDKQARQAIDEVDSHRLSYIEGLLIQAGVPSGDAQDRAYLIYAYQIS
                     LSLLHSEDTPQQRLERSQRMAGILIPESCTLPA"
     gene            4219..5034
                     /locus_tag="VIO83_12255"
     CDS             4219..5034
                     /locus_tag="VIO83_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010567583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8332084.1"
                     /translation="MSANPKTLLAAGHQTHYHEAGQGEALFLLHGSGPGVSGWANWAN
                     TVPVFADRWRVVVPDIAGFGFTEFKDDAKYDIKLWVNHLLGIMDALDIEKASFVGNSF
                     GGALAIGLAVFAPERVNKLVLLGTPAGEFVQTPGLRGAWEYEPSLDNMRELMKLFPYD
                     VSLITEAMVQARYEASARPGAQQALRKLIPQPAADGPTLVKGFPAAALAKIVAPTLVV
                     HGREDRVVPPECGLLIANSIPDADLHLFGRCGHWVQSEQPRRFAALVRDFLSE"
     gene            5056..5337
                     /locus_tag="VIO83_12260"
     CDS             5056..5337
                     /locus_tag="VIO83_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010567584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol ring-cleavage dioxygenase"
                     /protein_id="HEY8332085.1"
                     /translation="MERALWQLCVERVAKERFRQDAVGFLGRFALEEAEVRMIVEFDV
                     AGLQAAGVNPMLTMGFWQELAPDRNMSLYKQRLGATDEHSAGFSAALKG"
     gene            5369..6217
                     /locus_tag="VIO83_12265"
     CDS             5369..6217
                     /locus_tag="VIO83_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017847306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HEY8332086.1"
                     /translation="MPHDPVMFVAPDAPPLAQREAVWAAYEACAERLAALQPTSVIIV
                     GCDHYILFGTHCLPRYVIGTGDVDGPIDRLPGLERGAVRNHQALAAHIVEHGDGQGVD
                     WTVARSFTVDHSFSIPHQLVVKPAEALLGRELPSIPVYLACGVDPYISLQRSADLGRQ
                     LRAAVEAFDADERVVIIGSGGISHWVGTAEMGRVAEDFDREILALGVNGDLQALCAYS
                     DEEILRRAGNGAMEIRNFACALAAIERPQGEVIAYEAVPAWVTGLGFVQLSVRAEHEP
                     CITLPT"
     gene            6189..6542
                     /locus_tag="VIO83_12270"
     CDS             6189..6542
                     /locus_tag="VIO83_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017847307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY8332087.1"
                     /translation="MSRVLLCQRDELAPGELKKVAHREGDAICVYNLGGTFYATTDMC
                     THATASLAEGDLEDDLITCPVHWGQFHVPSGKAVTFPCERHLRTYKVIVTDREVFADI
                     ALEADEALAAANSPV"
     gene            6582..7931
                     /locus_tag="VIO83_12275"
     CDS             6582..7931
                     /locus_tag="VIO83_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017847308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8332088.1"
                     /translation="MDNNKHIPLRTIESTPALGNLDRICDMEEGRLSGKVFWDPAIYE
                     QELEKIFARCWLFVAHESQLPKSGDYLTTSMGEDEVLVVRQKDASIKVFLNACPHRGN
                     KVCFAEAGNARGFICNYHGWSFGADGGRLMGMHESKCYEESQFDKSRHGLREARVASY
                     KGLVFATFASDAPTLEEYLGPMTWYMDVMLDMDEGGSEFLGGCIRSTIECNWKIAAEN
                     FVGDILHGGWTHDSAAKAMLGGPVANVGELPESYSVNWNGHGYEFATDVVGNAATLGE
                     KDINKYLHVLRPKVADRLGEFRSRLIGAISSFTVFPNFSFLPGQNTVRIWQPRGPHKI
                     ELFTWVIVNKNAPQEIKDKWRRGAMMTFSPTGVFEMDDGENWEYCTKSSRGVVTRYQD
                     LYVGLGMHTRLENSELPGNVFKGQLNECNARAFYQRWKDLLQAPTWADVPDRNGTLR"
     gene            7941..8471
                     /locus_tag="VIO83_12280"
     CDS             7941..8471
                     /locus_tag="VIO83_12280"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017847309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HEY8332089.1"
                     /translation="MTHEQQDSLVSVEETRRVEQFLLREARLLDGECWDAWLHGLSEA
                     IHYWMPGIENRRREDKSGPYCLEHMAFFDDGMRELKRRVARFKQPSAWAENPPTRNVH
                     LISNIEVYAGPAPGEYLAYSCFINVRSRGLDEHHQIAGRREDVLRLEADGLKLLKRKI
                     LIPNAMLLCKNINTFL"
     gene            8482..9294
                     /gene="hcaB"
                     /locus_tag="VIO83_12285"
     CDS             8482..9294
                     /gene="hcaB"
                     /locus_tag="VIO83_12285"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010567589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(cis-5,6-dihydroxycyclohexa-1,
                     3-dien-1-yl)propanoate dehydrogenase"
                     /protein_id="HEY8332090.1"
                     /translation="MAWLKGQVALVTGGTGGIGSAIVRRYVAEGARVAVMGRDAAQLA
                     ALADTLGDAVITIEGDVSRYQDNQRAVAATLEAFGRLDTFVGNAAVFDYFTRLDRIAP
                     EEFEGAFQKLFATNVQGYLLGAQASIEALRETRGSMIFTLSNSAFYAGGGGILYVTAK
                     HALVGMIRQLAYELAPEIRVNGVAPGATNTPMKSLEGLNSRSVPLNQIPGFEAGAAQA
                     VPLQRIAEPEDHTGHYVLLASRENSGLTTANIIHSDGGWEIRSAGAAKPRKA"
     gene            9337..10308
                     /locus_tag="VIO83_12290"
     CDS             9337..10308
                     /locus_tag="VIO83_12290"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010567590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY8332091.1"
                     /translation="MTRTSLSAPQQQLLNLFAGLNLPVLAAPMFLVSGPALVAACCQA
                     GIVGSLPAPNARTVEQLQEWLEQISRERELAQQRGEQWAPWMLNMIVHSTYDRFDAEL
                     ELVRRYQPPIVSTALGSPKRVLETVHAYGGVVIADVISPALARKAVAAGVDGLVLVCN
                     GAGGHTGSYNPFAFVAEVRQFWDGPLGLAGAISNGRDIHAAQVLGCDFSLVGTRLIAA
                     HESLVEAAYRDMLVACSLDDVTTTKAVSGVLANWMRPSLEKAGIDPSAENRASIDFSG
                     DISTANKAWKHVWSAGQGVGQITGTYSVAQLVDELHQGYRESRARQL"
     gene            10338..11120
                     /locus_tag="VIO83_12295"
     CDS             10338..11120
                     /locus_tag="VIO83_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017847312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8332092.1"
                     /translation="MHHENLQAAAAALALARSSRSPCPRISERFDIQSLHDAYAVQEI
                     NTQAALAAGRRLSGRKIGLTSLAVQQQLGVDQPDFGMLFADMEYRDGETIDCTRLIQP
                     KVEGEIAFVLGRDLPHPDTTLGELLRAVDHVLPALEIVDSAIEDWKITLVDTVADNAS
                     SGLYVLGKTPVSLRGLDLALEGMLLEKNAAQAAIGVGAACMGNPLDAALWLARTMALT
                     GRPLQAGDVLLSGALGPMVPVVAGDSLRLRLTRLGEVSCRFG"
     gene            11229..13088
                     /locus_tag="VIO83_12300"
     CDS             11229..13088
                     /locus_tag="VIO83_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010567592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HEY8332093.1"
                     /translation="MYDFFNDPTQLAPAKVLYEARADGSFVLSSPEPLQPYARCVGQW
                     LEHWAVQTPQRAYLAERRGAGWFSLNYAQVRQRVGALAQGLLELDLPAGRPLLMLSEN
                     DLDQALLTLAALHVGIPVSTISVAYSRNTGSGCSKLKAIVEVLKPALIFVNDGDAYCR
                     AIELVNPDCPVVAARNAQEIRGALALPLLYKAESPAVMQAFARLDGDTPARYLMTSGS
                     TGAPKAVVNTHGMLCANQQAIAQCWRFLEHEELVVLDWLPWSHVFGANHNFNLVLRNG
                     GTLYIDDGLPLPGQIDRTVENLKSVRPTLYFNVPRGYEVLLPYLERDEVLARALFGRL
                     RMLFYAAAALPQSSWARLAACATKVRDTPLFFATEWGSTETSPVVTNVHFAADKAGII
                     GLPVPGTQLKFVPCGDKLEMRVRGPSVFPRYLKDPVSSLAAFDEEGFYRIGDAGRLVD
                     PQRPELGVQFDGRVTEDFKLSSGTWVSVGTLRPRLVSALAPYVSDCVICGHDRDVIGA
                     LIYPSPALRDLLGAAGQQMNGAQLAMQSEVRLALCSGLLALAKEFPASSQHAMRLVIL
                     DSPPSLNDGEITDKGYINQRMALSLRAEAVTRLYADSLEPSVILLAEVYGEAV"
     gene            13085..>13237
                     /locus_tag="VIO83_12305"
     CDS             13085..>13237
                     /locus_tag="VIO83_12305"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017847314.1"
                     /note="catalyzes the formation of acetyl-CoA from
                     acetalaldehyde; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HEY8332094.1"
                     /translation="MNPAKLKVAIIGSGNIGTDLMIKILRNAKHLEMAVMVGIDPASD
                     GLARAQR"
BASE COUNT         2435 a         4126 c         4362 g         2314 t
ORIGIN      
        1 cctccaggct caggttgagt tggcgtaccg cttcgacctt gccgtggctg atcgtcaggt
       61 tgcgtacatc cagcaggcga ctcattcgac acctccaagg taggcggcaa gcaccgcagg
      121 gttttgctgt atctgcagcg gcgtgccacc ggccaggcgc tggccgaatt ccatcaccac
      181 gatctggtcg gccaggccca tgacgaattc catgtcgtgc tccaccagca gcacgccgag
      241 gccttcgctg cgcagttgtt cgagcaggtg ggcgagtgcc tgcttttcgc catggcgcag
      301 tccggcggcg ggttcgtcga gcaacagcaa caggggatcg ctgcacagcg ctcgggcaat
      361 ttcgacgatg cgctgctggc ccaacggcag gctgccggcg ggggtgtgca gggcggcgcc
      421 caggcccacg cgttcgatct ggcgccgggc ttcggccagc agggcggctt cctcttcgcg
      481 ctccagatgc agcatcgagc gcagcagccc ggctttgccc cgggcatggg cgccgagggc
      541 gacgttttcc aggacgctca tgtccggcaa caaacgcacg tgctggaagg tgcggctgac
      601 gcccagtcgg gcgatggcac gcggcgacag gccgtcgatg cgctggccga gaaaaacgat
      661 ctggccggca ttgggcgcca gcaccccgga caacagattg aacagggtcg acttgccggc
      721 gccgttggga ccgatcagcg cggtgatttc ggcgccattg acggtcaagc ccatgtcctt
      781 gttggccacc agtccgccaa actgcttgag cagaccttct gcgaccatca acggctgccc
      841 gttggcgggc atcgtccggc ggggcagcgc atgggctggg tgctcgggca gcggacgggg
      901 ttgtgccgtc gggaacaggc cactcagtaa tggcaacagg ccgccacggg cgtattgcag
      961 gaccaggatc accagcacgc cgaagaaaat catctcgacg ttgtcggcgt gtccgaacag
     1021 ctgtggcagt acgtcttgca gcacctgttt gatcagggtc agcagggctg cgccgagcag
     1081 tgcgccccag aggctggcaa cgccgccgag cacgatcatg aacagatact cgatacccat
     1141 caccaccgag aacggcgtgg gattgaccac acgttgcagg tgcgcgtaca gccagcccga
     1201 caacgccgcg agcagggccg cgatgatgaa cacctgcaag cggctgcgcg ccgtgtcgat
     1261 gcccatcgcc tcagccatgc cggcggaatc cttgagggtg cggatggcgc ggccctggcg
     1321 gctgtgcagc aggttgtcga gcagccacag gctgatcgcc aacagcagcc caatcagcag
     1381 gctgaagtcg gccgggtcgc ggacctgcca gccgaacagg ctgatattcg gcaggttttg
     1441 caggccgccg aagccgccca gcccgggcag gttgccgaac aggtaaaaca gcgccaggcc
     1501 ccaggccagg gtaccgatgg gcaggtagtg gccggccagg cgcagggtca acagacccag
     1561 gctggtggca accccaaggg taatcgccag gcccgcaagc aaaccgagcc agggcgacag
     1621 gccatattgg gtggtcagca ccgcgctggc gtaggcgccg ataccgacga acgcggcctg
     1681 gccgaacgag gtcatgccgc cgatgccggt cagcagcacc aggcccaggg cgaccaggct
     1741 gtacaggccg atgtaattca gcagcgtgac atggaaggtc ggcagcagca ctggtgccag
     1801 gcccgccaga agcagcaggg cgaacagggg gatcagtcga cggatcatca gtgttgctcc
     1861 tcaaggtgag ggtgcttcaa cgagagccac agcagtaccg ggatcaccag ggtgaacacg
     1921 atcacctcct tgtaggccga ggcgccgaag ttggcgaagc tctccaggac accgacgaaa
     1981 aacgcactgg ccgcggcaat cgggtaactg gccaggccgc cgaagatcgc agcgacaaac
     2041 cccttgaggc tgatcagaaa gcccgagtcg tagtacaggg tggtcagcgg gccgatcagc
     2101 acgccggaaa tggcgccgat caacgccgcc aggccgaacg ctgccttgcc ggccagctgt
     2161 gtggggatgc ccaccagttg cgcgcccttg cgattcagtg ccgtggcgcg cagggccttg
     2221 ccatacaggc tgtggccgaa gaacgcgtag agcaggccga tcagcagcag ggcgctggcg
     2281 atcaccaaca ggctttgcag gcccaccggc agctcaccga gcatgaacac cgcttcggcc
     2341 agggcatcgg tgcgaacccc ttctgcgccg aacatcagca agctgatacc caccagcgtg
     2401 atgtgtacag ccaccgatac gatcagcagc aacagcaccg aggtctcggc cacccgttga
     2461 tacaccaggc gatacagcag cggccccagc ggcgtgacca gcgccagggt cagcaggatc
     2521 tgcaacacgg ccggcaaacc ggccagctcg aaggtgtcca gcaccaccca cagcaccgtc
     2581 ggcaagccca ggtagcgtgc cagcaggccg ggcacccggc gcgattgacc acggcgcaac
     2641 gcggcgatgc tgtccagtgt gcaggcggcc agcgagcagc cgagcagcag ccacagggtg
     2701 ccgggcacca gccccagttg cagggtggcc agggtcaggg cgctgtaggt gacgaagtcg
     2761 ccctggcaca ccaggatggt gcgcgtcacc gagaacacca gcaccagggc cagcgccacc
     2821 aaggcataaa tcgcgcccgt ggtcaggccg tcctgggcga gaaacgccag tatcgagata
     2881 tccatgagcg gccctcagcg agcggtagga aagggaggcg aaggtgtgcg ccagcgggcg
     2941 tcggtcaatg ccgtgccggg gtgctgtcgt cgggagggcg aggtgtcaag cgcgcagggt
     3001 gtggccgtgc gcaggggtgc atgcaagtgc atagtgaggt acccgttttc tattcttatt
     3061 gtcgtgtgga taagcctgag ggagcgccat acacagcttt aaaatacaca tgtgaatgtg
     3121 tatgtataga ctacgcggga gggtgggcgc tttgtcaata ggcgccgctg taacggtttg
     3181 tttaaaggcg ggtttctaat ctatgttgtt gtttttagat aaaaaaataa aataatcttt
     3241 ttcatctgaa aagctcggaa cccagtgcgc gtgctgcttt gtgatcttga aagatacatt
     3301 catgtatttt tatgaatgcc gacgcaggtc ggcatccggg ctcaggcggg gagggtgcag
     3361 gattcgggga tcaggatgcc ggccatgcgc tggctgcgtt ccaggcgttg ttgcggggtg
     3421 tcttcgctgt gcagcaatga caggctgatc tggtaggcat agatcaggta ggcgcggtcc
     3481 tgggcatcgc cactgggcac gccggcctgg atcagcaggc cttcgatgta gctcaggcgg
     3541 tggctgtcga cttcgtcgat ggcctggcgc gcctgcttgt cgcggcgggc ccagccgcga
     3601 atgcccagct cgatcgaggc ggcgtccttg gcggtctgcc cgtgggaggg cagttcgagc
     3661 aggcgatgca gttgttgcag cggcgagctg tgggcattgc gcaggtgcag aatcacatct
     3721 tcggtggcgc gtgcgcgcca gttgccgagg atgccttcga gcagttcccc gcgacttttg
     3781 aagtggtagt agaagctgcc acgggtgatc ttcaattcct tggccagggt atcgacccgc
     3841 acagcgtcga cgccgctggt aaccaggact tcctgtgccg catgaatcca atcttcccgc
     3901 gtgaggttgg tgcgtgatga cattgtctag gggccttgtc caacaagctg gataagcgcc
     3961 agggcacagc agcgttcgac gcccgccttg gcagcgagcc gccattctaa cgcccggcgc
     4021 aacgacgaag ctattgccag cgcaccgact gtggcccgcg catgggggct gcgtgtgctc
     4081 ggattctaga gtacatatgt gtaggttggc ttttagatta tttgcacaaa gccacgtttt
     4141 tttaattgac gcaggtcaaa tcggtaaata gcatacagat acacatctgt atgttaagaa
     4201 taagaaccgg aggccgacat gagcgcaaat cccaagactc tgcttgcagc agggcaccag
     4261 actcactacc acgaagccgg tcagggcgag gccttgttcc tgttgcatgg ttcgggcccc
     4321 ggtgtatcgg gctgggccaa ctgggccaat accgtgccgg tgttcgctga tcgctggcgc
     4381 gtggtcgtgc cggacatcgc cggcttcggc ttcaccgagt tcaaggacga cgccaagtac
     4441 gacatcaagc tgtgggtcaa ccatctgctc ggcatcatgg atgccctgga catcgaaaaa
     4501 gcctcgttcg tcggcaattc gtttggcggc gcgctggcca tcggcctagc ggtgttcgca
     4561 ccggagcggg tcaacaagct ggtgttgctc ggcaccccgg ccggtgagtt cgtgcagacc
     4621 ccaggcctgc gcggcgcctg ggaatacgag ccatcgctgg acaacatgcg cgagctgatg
     4681 aagctgttcc cctatgacgt atcgctgatt accgaggcga tggtgcaggc acgttacgaa
     4741 gccagtgcgc gccctggtgc ccagcaggcg ctacgcaaac tgatcccgca gccggctgcc
     4801 gacggcccga ccctggtcaa gggttttccc gcggccgcgt tggccaagat cgtcgcgccg
     4861 acgctggtgg tacacggccg tgaagaccgc gtggtgccgc ccgagtgcgg gctgctgatc
     4921 gccaacagca ttcccgatgc cgacttgcac ctgttcgggc gctgcgggca ctgggtgcag
     4981 agcgagcagc cgcgccgctt tgccgcgctg gtccgcgatt tcctcagcga gtaggccggt
     5041 atgagcagaa acgttatgga gcgtgcgctc tggcagctgt gcgtcgagcg tgtcgccaaa
     5101 gagcgctttc ggcaggacgc ggtcgggttc ctcgggcgtt ttgccctgga ggaggccgag
     5161 gtccgcatga tcgtcgagtt cgatgttgcc gggctgcagg cggccggggt caacccgatg
     5221 ctgaccatgg gcttctggca ggagcttgcc cctgaccgca acatgagcct ctacaaacag
     5281 cgcctgggcg ctaccgacga acacagcgcg ggattttccg ccgcgctcaa ggggtgaacc
     5341 gatgggcaag atagtaggcg gcttctggat gccgcatgac ccggtaatgt tcgtggcacc
     5401 cgacgcgccg ccgttggcgc aacgtgaggc ggtatgggct gcatacgaag cctgcgccga
     5461 acgcctggcg gccttgcagc cgacctcggt gatcatcgtc ggttgcgacc attacattct
     5521 gttcggcacc cattgcctgc cgcgctatgt gatcggcacc ggcgatgtgg atggccccat
     5581 cgatcgcctg ccgggcctgg aacgtggcgc ggtgcgcaac catcaagcgt tggccgcgca
     5641 cattgtcgaa cacggcgatg ggcagggcgt ggactggacc gtggcgcgca gttttaccgt
     5701 cgaccattcc ttttccattc cgcaccaact ggtggtcaag ccggccgagg ccttgctcgg
     5761 gcgtgagttg ccgagcattc cggtgtacct ggcgtgtggc gttgacccgt acatctcgct
     5821 ccagcgttcg gccgacctcg gccggcagct gcgggcggcc gtcgaagcgt tcgatgccga
     5881 cgagcgtgtg gtgattatcg gcagtggcgg tatcagccac tgggtcggca ccgccgagat
     5941 gggccgggtg gccgaagact tcgatcgcga gattctcgcc ctcggcgtca atggcgacct
     6001 gcaagcgctg tgcgcctaca gcgacgaaga gatcctgcgt cgggccggca acggcgcgat
     6061 ggaaatccgt aacttcgcct gcgccctggc cgccatcgag cgcccgcagg gcgaagtgat
     6121 tgcctatgaa gccgtacccg cctgggtcac cgggctgggc tttgtgcaac tgagtgtgag
     6181 ggccgagcat gagccgtgta ttactttgcc aacgtgatga gctggcgcca ggggagctga
     6241 agaaggttgc ccaccgcgag ggcgacgcca tctgcgtcta caacctgggg ggcacgttct
     6301 acgccaccac cgacatgtgt acccacgcca ccgcttcgct ggccgagggc gacctggaag
     6361 acgacctgat tacctgcccg gtccactggg gccagttcca tgtgcccagc ggcaaggcgg
     6421 tgacgtttcc ctgcgagcgc cacttgcgca cctacaaggt catcgtcacc gaccgcgagg
     6481 tgttcgccga catcgccctg gaggctgacg aggcgctggc cgccgccaac agcccggtgt
     6541 gaccgggccg ctgtccaacc gtattcctgt gagtctgaac catggataac aacaaacaca
     6601 taccgctcag aaccattgaa agcacgcccg ccctgggcaa cctcgaccgt atctgcgaca
     6661 tggaggaagg gcgcctgtcc ggcaaggtct tctgggaccc ggcgatctac gagcaggagc
     6721 tggagaaaat cttcgcgcgc tgctggctgt tcgtcgcgca cgagtcccag ctgcccaagt
     6781 ccggcgacta cctgaccacc tcgatgggtg aagacgaagt gctggtggtg cgccagaagg
     6841 acgcctcgat caaggtcttc ctcaatgcct gcccgcaccg tggcaacaag gtgtgctttg
     6901 ccgaggccgg caacgcccgc ggctttatct gcaactacca tggctggtcg ttcggtgccg
     6961 atggcggccg gctgatgggc atgcacgagt ccaaatgcta cgaagaaagc cagttcgaca
     7021 agtccaggca cggcctgcgc gaggcgcgtg tcgccagcta taaaggcctg gttttcgcca
     7081 cgtttgccag cgatgcgccg accctggagg aatacctcgg gccgatgacc tggtacatgg
     7141 acgtgatgct cgacatggat gaaggcggca gcgagtttct cggtggttgc atccgctcca
     7201 ccattgagtg caactggaag atcgccgccg agaacttcgt cggtgacatc ctgcacggcg
     7261 gctggaccca cgattcggcg gccaaggcca tgctcggcgg gccggtggcg aatgtcggcg
     7321 agttgcccga gtcgtactcg gtgaactgga atggccacgg ctatgagttc gccaccgacg
     7381 tggtcggcaa cgccgcgacc ctgggcgaga aagacatcaa caaatacctg cacgtgctgc
     7441 gtcccaaggt ggcggaccgc ctcggcgaat tccgctcgcg gctgatcggg gcgatttcct
     7501 cgttcaccgt gttccccaac ttttcctttc tgccggggca aaacaccgtt cgcatctggc
     7561 agccgcgcgg cccgcacaag atcgagctgt tcacctgggt gatcgtcaac aaaaacgccc
     7621 cgcaggagat caaggacaaa tggcggcgcg gcgcgatgat gacgttctcg cccaccgggg
     7681 tgttcgagat ggacgacggc gagaactggg agtactgcac caagagcagc cgtggcgtgg
     7741 tcacgcgtta tcaggacctg tacgtcggcc tgggcatgca cacccgtctc gaaaacagcg
     7801 agttgcccgg caatgtgttc aagggccagc tcaacgaatg taatgcgcgg gcgttctatc
     7861 agcgctggaa ggacctgctg caggcaccga cctgggccga tgttccggac cgcaacggca
     7921 cgctgcgctg agggggccag atgacacacg aacaacagga cagcctcgtt tcagtcgagg
     7981 aaacccgccg cgtcgaacag ttcctgctgc gcgaagcgcg cctgcttgac ggcgaatgct
     8041 gggacgcgtg gctgcacggt ctcagcgaag ccatccacta ctggatgccg ggcatcgaga
     8101 accggcgccg cgaggacaaa tccgggccct attgcctgga acacatggcc ttcttcgatg
     8161 acggcatgcg cgaactcaag cgccgcgtgg cacgcttcaa gcagccgtcg gcctgggccg
     8221 agaacccgcc gacgcgcaac gttcacctga tcagcaacat cgaagtgtat gccggcccgg
     8281 cgccgggcga atacctggcc tactcgtgct ttatcaacgt gcgcagccgc ggcctggacg
     8341 agcatcacca gattgccggc cggcgcgagg atgtactgcg cctggaggcc gacggcctca
     8401 agttgctcaa gcgcaagatc ctgatcccta acgcgatgtt gctgtgcaag aacatcaaca
     8461 ccttcctctg agggctggca tatggcttgg ttaaaagggc aggtggcatt ggtcaccggc
     8521 ggtaccgggg ggattggcag cgccatcgtc cgtcgttacg tggccgaggg cgcgcgcgtc
     8581 gcggtcatgg gccgcgatgc ggcgcaactg gccgcgttgg ctgacacgct tggcgacgcg
     8641 gtcatcacga ttgaggggga tgtgtcgcgg tatcaggaca atcagcgtgc agtggcggcg
     8701 acccttgagg cgtttggtag gcttgacacc ttcgtcggca acgctgccgt gttcgattat
     8761 ttcacgcgcc tggaccgtat tgccccggaa gagttcgagg gcgccttcca gaaactcttc
     8821 gccaccaacg tgcagggtta tctgctgggc gcgcaagcct cgatcgaggc actgcgcgaa
     8881 acccggggca gcatgatctt taccctctcc aacagtgcct tttatgccgg cggcggtggc
     8941 attctctacg tcaccgccaa gcatgcgttg gtgggcatga ttcgccagct ggcttacgag
     9001 ctggcgccgg agatccgcgt caacggcgtg gctccggggg cgaccaacac gccgatgaaa
     9061 agcctggaag gcttgaacag ccgcagtgtg ccgctgaacc agatcccggg cttcgaagcc
     9121 ggtgcggcgc aggcggtgcc gttgcaacgg attgccgagc ccgaagacca caccgggcac
     9181 tacgtgctgt tggcctcgcg ggaaaattcc ggcctgacca cggcgaacat catccacagc
     9241 gatggcggct gggagatccg cagtgccggt gcagccaagc cgcgcaaggc ctgagccagg
     9301 ccgacgcctt gataactaga ataagagaga tgcgtgatga caagaacaag cctgagtgca
     9361 ccgcaacagc aactcctgaa cctgtttgcc gggcttaacc tcccggtcct cgcggcgccg
     9421 atgttcctgg tgtcggggcc ggccctggtg gccgcctgct gccaggccgg catcgtcggc
     9481 agccttccgg cgcccaatgc gcgaacggtc gagcaattgc aggaatggct ggagcagatc
     9541 agccgcgagc gtgagctggc gcagcaacgc ggcgagcagt gggcgccatg gatgctcaac
     9601 atgatcgtac acagcaccta cgaccgcttc gacgctgagc ttgagttggt gcgccgttat
     9661 cagccgccca tcgtgtccac agccctgggt agccccaagc gcgtccttga aaccgtacac
     9721 gcttatggcg gcgtggtgat cgccgacgtg atcagcccgg cactggcgcg caaggcggtg
     9781 gccgctggcg tcgatggcct ggtcctggtg tgcaacgggg cgggcgggca taccggcagc
     9841 tacaacccgt tcgccttcgt cgccgaagtg cgccagttct gggacggccc tctgggcttg
     9901 gccggggcga tttccaacgg ccgcgacatc catgcggcgc aggtattggg ctgcgatttc
     9961 tcgctggtcg ggacccgtct gattgccgcc cacgaaagcc tggtggaagc cgcctaccgc
    10021 gacatgctgg tggcctgctc gctggatgac gtgaccacca ccaaggcggt cagcggcgtg
    10081 cttgccaact ggatgcggcc gtcgctggaa aaggccggta tcgaccccag cgccgaaaac
    10141 cgcgccagca tcgacttttc cggtgacatc tccactgcca acaaggcctg gaaacatgtg
    10201 tggtcggccg gccagggcgt aggccagatc accggcacct acagcgtggc gcaactggtg
    10261 gacgagttgc accagggtta tcgcgaaagc cgcgcacgtc agttgtgagc catccagaac
    10321 cgttcaggag caaccccatg caccacgaaa accttcaggc cgccgccgct gcgctggccc
    10381 tggcgcggtc gagccgcagc ccttgcccac gcatttccga gcgtttcgac atccagtcgt
    10441 tgcacgacgc ctacgccgtg caagagatca acacccaggc cgccctggct gccggtcggc
    10501 gcctgagcgg gcgcaagatt ggcctgacgt ccctggcggt acagcagcag ttgggcgtcg
    10561 atcagcccga tttcggcatg ctgtttgccg acatggaata ccgcgatggc gagaccatcg
    10621 actgcacccg gttgatccag cccaaggtcg agggcgaaat tgccttcgtc ctggggcgcg
    10681 atctccctca ccccgatacg accctgggcg aattgctgcg cgccgtcgac cacgtgctgc
    10741 cggctctgga aatcgtcgac tcggcgatcg aggactggaa gatcaccctg gtcgataccg
    10801 tggccgacaa cgcttcatcg ggcctctacg tgctgggcaa gactcctgtc agcctgcgcg
    10861 gcctggacct ggcgctcgaa ggcatgctgc tggagaagaa cgcggcccag gcggccatcg
    10921 gcgtgggggc cgcgtgcatg ggcaacccac tcgacgctgc gctgtggctg gcgcgcacca
    10981 tggccctgac cgggcgcccg ttgcaggcgg gcgacgtgct gttgtcgggc gcgctcgggc
    11041 ctatggtgcc ggtggtggcg ggcgacagcc tgcgcttgcg cctcacccgc ctgggtgaag
    11101 tgagctgtcg tttcggctga ggccggcagg caacaaccct gctaccgggc tgacgacccg
    11161 gtggcgatta caacaataaa acgagtgagc agcgggctga tggcctgccg tattcacgag
    11221 ggcgtgcaat gtacgatttt ttcaatgatc cgacccaatt ggcccctgcc aaggtgctgt
    11281 atgaggcacg tgccgacggc agcttcgtgc tgagttcacc cgagcccctg cagccctatg
    11341 cgcgctgtgt cgggcagtgg ctcgaacact gggccgtgca gacaccgcag cgtgcctacc
    11401 tggccgagcg gcgcggcgcc ggctggttca gcctgaacta tgcccaggtg cgccagcgcg
    11461 tgggggcatt ggcccagggc ttgctcgagc tggatctgcc ggccgggcgc ccgctgctga
    11521 tgctctcgga aaacgatctg gaccaggcgc tgttgacctt ggcggcgctg cacgtgggca
    11581 tcccggtgtc gacgatttcc gtggcgtact cgcgcaacac cggcagcggg tgcagcaaac
    11641 tcaaggcgat tgtcgaagtg ctcaagcctg cgctgatttt cgtcaacgac ggcgatgcct
    11701 attgccgtgc catcgagttg gtcaacccgg attgcccggt ggtggcggcg cgcaacgccc
    11761 aggaaattcg cggggcgctg gccttgccgc tgttgtacaa ggccgaaagc ccagcggtga
    11821 tgcaagcgtt tgcacggctc gacggcgaca ccccggcgcg ctacctgatg acctccggtt
    11881 cgaccggtgc gcccaaggcg gtggtcaata cccacggcat gctctgcgcc aaccagcagg
    11941 cgattgccca gtgctggcgc tttcttgaac acgaagaact ggtggtgctc gactggttgc
    12001 cctggagcca tgtgttcggc gccaaccaca acttcaacct ggtactgcgc aacggcggca
    12061 ccttgtacat cgatgacggt ctgccgttgc cggggcaaat cgaccgcacc gtggaaaacc
    12121 tcaagtcggt gcgacccacc ctgtacttca acgtgccgcg cggctacgag gtcttgctgc
    12181 cgtacctcga gcgcgacgag gtactggcgc gcgccttgtt cgggcgcctg cgcatgctgt
    12241 tctacgccgc tgccgcgctg ccccagagca gttgggcgcg gctggcggcg tgtgccacca
    12301 aggtccgcga caccccgctg ttctttgcca ccgagtgggg ctcgaccgag acgtcgccgg
    12361 tggtcaccaa cgtgcatttc gccgccgaca aggccggcat catcggcctg ccggtgccgg
    12421 gcacgcagct caagttcgtg ccctgcggcg acaagctgga gatgcgcgtg cgcgggccgt
    12481 cggtgttccc gcgctacctg aaggacccgg tcagcagcct ggccgcgttc gatgaggagg
    12541 gcttctaccg cattggcgac gcggggcgct tggtcgaccc gcagcggccg gagttggggg
    12601 tgcagtttga cggtcgggtg actgaagact tcaaactcag cagcggcacc tgggtatcgg
    12661 tgggcaccct gcgtccgcgc ctggtcagtg ccttggcgcc ttacgtcagc gattgcgtga
    12721 tctgtgggca tgatcgcgac gtgatcggtg cgctgatcta cccgagcccg gccctgcgcg
    12781 atctgctcgg tgccgccgga caacagatga acggcgcaca actggcgatg caatccgagg
    12841 tgcgtctggc cttgtgttcg gggctgctgg cgctggccaa ggaattcccg gcgtcgtccc
    12901 agcatgccat gcgcctggtg attctcgact cgccgcccag cctcaacgac ggcgaaatca
    12961 ccgacaaagg ctacatcaac cagcgcatgg cgctgagcct gcgcgccgag gccgtgacgc
    13021 ggctgtacgc cgacagcctc gagcccagcg tcatcctcct cgctgaagtc tacggagaag
    13081 ccgtatgaac cctgcaaaac tcaaagtcgc catcatcggt tccggcaaca tcggcaccga
    13141 cctgatgatc aagatcctgc gcaacgccaa acacctggaa atggcggtca tggtcggcat
    13201 cgacccggcc tccgacggcc tcgcccgcgc ccagcgc
//
LOCUS       DATCUN010000332         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_36788, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000332 DATCUN010000000
VERSION     DATCUN010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36788"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VIO83_12310"
     CDS             <1..697
                     /locus_tag="VIO83_12310"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY8332095.1"
                     /translation="LGRVRLFELGRSFHPQAGEGTPAPLETQRVAAAVCGDADPQQWG
                     LATRKVDFHDLKGDLESLAAASGAVLTFQPSQRPYGHPARSAEVLRDGVSLGWIGQIH
                     PRLAKAMDIDVDVFAFELDLAPLAARQLPRAAELSRFPSVRRDLAFLVPEAAAWADLE
                     ATLRRAVGPLLRQVTLFDRYVGQGVEPGFKSLAMGLILQDKSRTLTDRDVESVVAEAV
                     SAMEREHHARIRG"
     gene            723..1022
                     /locus_tag="VIO83_12315"
     CDS             723..1022
                     /locus_tag="VIO83_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEY8332096.1"
                     /translation="MALTKAEMAEKLFDEVGLNKREAKEFVDAFFDVLREALEQGRQV
                     KLSGFGNFDLRRKNQRPGRNPKTGEEIPISARTVVTFRPGQKLKERVEAYAGSGQ"
     gene            1003..1359
                     /locus_tag="VIO83_12320"
     CDS             1003..1359
                     /locus_tag="VIO83_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8332097.1"
                     /translation="MLDPGSNRELPPIPAKRYFTIGEVSELCDVKPHVLRYWETEFPS
                     LEPAKRRGNRRYYQRHDVLMVRQIRGLLYEQGYTIGGARLRLEGAGARDESTLSNQII
                     KQTRMELEEVLQLLRR"
     gene            1427..1503
                     /locus_tag="VIO83_12325"
     tRNA            1427..1503
                     /locus_tag="VIO83_12325"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1461..1463,aa:Pro,seq:ggg)
BASE COUNT          264 a          481 c          515 g          252 t
ORIGIN      
        1 gctgggccgg gtgcgcctgt tcgagctggg ccgcagcttc cacccgcagg caggcgaggg
       61 caccccggca ccgctggaga cccagcgcgt ggccgccgcc gtgtgtggcg acgccgaccc
      121 gcagcagtgg ggcctggcca cccgcaaggt cgatttccac gacctcaagg gtgacctgga
      181 atcgctggcg gcggcctccg gcgcggtgct gaccttccag ccctcgcagc gcccgtacgg
      241 ccacccggcg cgctcggccg aggtgctgcg cgatggcgtc agcctgggct ggatcggcca
      301 gatccacccg cgcctggcca aggccatgga catcgacgtg gacgtgttcg ccttcgagct
      361 ggacctggcc ccgctggccg cccgccagct gccgcgtgcc gccgagctgt cgcgcttccc
      421 gtcggtgcga cgtgatctgg ccttcctggt gcccgaagcc gccgcctggg ccgacctgga
      481 agccaccctg cgccgggccg tcgggccgct gctgcggcag gtgaccctgt tcgaccgtta
      541 cgtcggccag ggggtcgagc cgggcttcaa gagtctcgct atgggcttga ttttgcagga
      601 caagtcgcgc actctgacgg accgcgacgt ggagtcggta gtggctgagg ctgtctctgc
      661 catggagcgt gaacaccacg cccggatccg cggttgagcg ggcagcatgg ggggtagcag
      721 gaatggcatt gaccaaggct gagatggccg aaaagctgtt cgacgaagtg ggtctgaaca
      781 agcgcgaagc caaggaattt gtcgacgcgt tcttcgatgt gttgcgtgag gcattggaac
      841 agggacgtca ggtgaagctg tccggcttcg gtaacttcga cctgcggcgc aagaaccagc
      901 gcccgggtcg caacccgaag accggcgagg aaattccgat ttccgcccgt acggtggtca
      961 ccttccgtcc gggccagaag ctcaaggaaa gggtagaggc atatgctgga tccgggcagt
     1021 aaccgcgaac ttccgccgat accggcaaag cgctacttca ccattggtga agtcagcgag
     1081 ctttgcgatg tcaaaccgca cgtgctgcgc tactgggaaa ccgagtttcc cagcctggag
     1141 ccggccaagc gccgcggcaa ccgtcgctac taccagcgcc acgacgtgct gatggtgcgc
     1201 cagatccgcg gcctgctcta cgaacagggc tacaccatcg gcggcgcgcg cctgcgcctg
     1261 gaaggtgccg gtgcccgcga cgaatcgacg ctgagcaacc agatcatcaa gcagacgcgg
     1321 atggagctgg aagaagtgct gcaattgctt cgtcgctgaa caaattcttc gataaaaccg
     1381 ttatactcgt cggcccgctg agaggcgggg acagtaccag catcatcggg gcgtagcgca
     1441 gcctggtagc gcatctgcct ggggggcaga gggtcgtcgg ttcaaatccg gccgtcccga
     1501 ccattgttgg ta
//
LOCUS       DATCUN010000333         5374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_18089, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000333 DATCUN010000000
VERSION     DATCUN010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18089"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VIO83_12330"
     CDS             complement(<1..500)
                     /locus_tag="VIO83_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HEY8332098.1"
                     /translation="MQPIKRLTPFIAVAGQLEPADMGTLAASGYRCVINNRPENEGEG
                     QPSSAEMRTAAETSGLEYHDLPVISGKISDEDVADFRELLDQVKGPVVAFCRTGTRST
                     TLWALAEAHHLDPEALLATAKEAGYDLAALQPRLEQRWQTLPNQPQPAAAYAHTPRYD
                     VLVIGGG"
     gene            complement(534..1400)
                     /locus_tag="VIO83_12335"
     CDS             complement(534..1400)
                     /locus_tag="VIO83_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8332099.1"
                     /translation="MNAHVEAFFDPATFTYSYVVSDPDSRQCALIDPVLDYDPAAGRT
                     SHQNADRLVAYVREHGLTVQWILETHVHADHMSAGHYLKEQLGGQLGIGDQIPVIQQT
                     FGKLFNVEPEFATDGRQFDHQFHDGETFRVGNVEARAIHTPGHTPACMTYLIGDAAFV
                     GDTLFMPDYGTARCDFPGGDARTLYHSIHAKLFSLADDTRVFMCHDYKAPGREEYLYE
                     TTIGAEREHNVHIHQGIDEEAFVAMRTARDATLGMPTLILPSVQINMRAGELPPAEDN
                     GTRYLKIPLDLL"
     gene            complement(1638..1952)
                     /locus_tag="VIO83_12340"
     CDS             complement(1638..1952)
                     /locus_tag="VIO83_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY8332100.1"
                     /translation="MSTVAKVIETMQARFNPGAATGLDLVFQFKIEDAENYYLSIKNG
                     ACTLTAGDAPAPNVALIMDSATLKGVLKGEISGMEAFMSGRLRTEGDMMLALKLGELF
                     PS"
     gene            2177..3199
                     /gene="sohB"
                     /locus_tag="VIO83_12345"
     CDS             2177..3199
                     /gene="sohB"
                     /locus_tag="VIO83_12345"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEY8332101.1"
                     /translation="MEFFTDYAGFLARAVTILAAILVVLASIAALRTRERRAGGQLDV
                     HKLNDFYKQLRERLQDSVLDKEQLKALRKEETKAEKAEKKAGSHKPRVYVLDFDGDIK
                     ASAGEHLRHEVTALLSLATPQDEVVVRLESGGGMVHSYGLASSQLARIRQAGVPLTVC
                     VDKVAASGGYMMACIGEKILAAPFAILGSIGVVAQLPNLHRLLKKHAIDFEVLTAGEY
                     KRTLTVFGENTDKGREKFQEDLDITHELFKNFVARYRPQLAIDEVATGEVWLGQAALG
                     KQLVDELMTSDEYLSERAQHAELFQLHYHEKKSLQERVGLATAQVLDGVLLRWWSRLS
                     QPRFWQ"
     gene            3225..4220
                     /locus_tag="VIO83_12350"
     CDS             3225..4220
                     /locus_tag="VIO83_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HEY8332102.1"
                     /translation="MSRFKALLVTESSSGQFDQAIVERELAELPAGEVLIRVRYSSLN
                     YKDALSATGSRGVTRAYPHTPGIDAAGEVVECATGEFQPGDQVIVTGYDLGVNSPGGF
                     AQYIRVPAAWVVRCPAGMTPREAMLLGTAGLTAGLCVEKLMHVGLDATAGSVLVTGAC
                     GGVGSVAVLLLTHLGYTVAASTGKLQQVEFLHRLGAQQIIDRAVLREGTEKPLLKMQW
                     AGAVDSVGGDLLFNVVKSLQYGASVACCGLTAGADMATASVLPFILRGVNLLGVDSVE
                     LPRAVKAAMWGKLAGPWKLETLEQLAREITLEELPEAISQILAGRMVGRMLVRID"
     gene            complement(4432..4926)
                     /locus_tag="VIO83_12355"
     CDS             complement(4432..4926)
                     /locus_tag="VIO83_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017905839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEY8332103.1"
                     /translation="MQRIIKNGEVIDERWHLLPVDASLEGISNSDDLIVPLGLWLASS
                     HALKVRDGGLGVWLQSHEQIEDIVDDLKHFQVIALDFPAFTDGRHFSSARLLRERYGY
                     QGEIRAIGDVLRDQLFFMQRCGFDAFAVRPDRDPYDALKGLSDFSVTYQTGVDQPLPL
                     FRRR"
     gene            complement(4910..>5374)
                     /locus_tag="VIO83_12360"
     CDS             complement(4910..>5374)
                     /locus_tag="VIO83_12360"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEY8332104.1"
                     /translation="LGLATPNIGLLTDIICCPGGDFCSLANAKSIPIAEAIQRRFDDL
                     DYVFDLGEIDLNISGCMNACGHHHIGNIGILGVDKKGAEFYQVSLGGEAGRNASLGQI
                     LGPSFAQDDMADVIAKVIEVYVEKRNEEERFIDTFRRIGIDSFKERVYAKNH"
BASE COUNT          865 a         1618 c         1918 g          973 t
ORIGIN      
        1 ccgccaccga tcaccagcac gtcgtagcgc ggggtgtgcg cgtaggccgc cgccggctgg
       61 ggctggttgg gcagggtctg ccagcgctgc tcgaggcgcg gctgcagggc ggcaaggtcg
      121 tagccggcct ccttcgccgt cgccagcagg gcctcgggat cgaggtgatg ggcctcggcc
      181 agcgcccaca gggtggtgga gcgggtgccg gtgcggcaga aggccactac cggccccttg
      241 acctggtcga gcagttcgcg gaagtcggcg acgtcctcgt cgctgatctt gccggaaatc
      301 accggcaggt cgtggtactc gaggcccgag gtttcggccg cggtgcgcat ctcggcgctg
      361 ctcggctggc cttcgccttc gttttccggg cggttgttga tcacgcagcg gtagccgctg
      421 gcggccaggg tgcccatgtc ggcaggttcg agctggccag ccacggcgat gaacggggtg
      481 agacgcttga tcggttgcat cttgttgttc tcctcagggt tgcctgggcc gaatcagagc
      541 aggtcgagcg ggatcttcag gtagcgggtg ccgttgtcct cggctggcgg cagttcgccg
      601 gcgcgcatgt tgatctgcac cgagggcagg atcagcgtcg gcatgccgag ggtggcgtcg
      661 cgggcggtac gcatggccac gaaggcttct tcgtcgatgc cctggtggat gtgcacgttg
      721 tgctcgcgct cggcgccgat ggtcgtttcg tacaggtact cctcgcgccc cggcgccttg
      781 tagtcgtggc acatgaacac ccgggtgtcg tccgcgaggc tgaacagctt ggcgtggatc
      841 gagtggtaca gggtgcgggc gtcgccgccg gggaagtcgc agcgggcggt gccgtagtcg
      901 ggcatgaaca gggtgtcgcc gacgaaggcg gcatcgccga tcaggtaggt catgcacgcc
      961 ggggtgtggc cgggggtgtg gatggcgcgg gcctcgacat tgccaacccg gaaggtctcg
     1021 ccgtcgtgga actggtggtc gaactggcgg ccatcggtgg cgaattcggg ttcgacgttg
     1081 aacagcttgc cgaaggtctg ctggatcact gggatctggt cgccgatgcc cagctggccg
     1141 ccgagctgct ccttgaggta gtggccggcg ctcatgtggt cggcatgcac atgggtttcc
     1201 aggatccact gcaccgtcag gccgtgctcg cgcacgtagg cgaccagccg gtcggcgttc
     1261 tggtgcgagg tacgcccggc ggcgggatcg tagtccagca ccgggtcgat cagcgcgcac
     1321 tggcggctgt ccgggtcgct caccacgtag ctgtaggtga aggtggcggg atcgaagaag
     1381 gcttcgacat gggcgttcat ggggcggtcc tccgcggcag ttcagatacc cgtaggggta
     1441 tggtgtgaac acagcctact gcgatttttg gttctatgca tagaataaaa acttatatat
     1501 gaagtcggcc atgatccgct ccccagtgtg gaccatgctg gcgttgctcg gggtctgtgg
     1561 cgggtgcggg agaggctgtc cggcggaaat gaaaaagccg gagcaagggc tccggctttg
     1621 ctgggacagg gcgcgaatca ggaggggaag agttcgccca gtttcagggc cagcatcatg
     1681 tcgccttcgg tgcgcagccg accgctcatg aaggcctcca tgccgctgat ctcgcctttc
     1741 aggacgccct tgagggtggc gctgtccatg atcagagcga cgttgggcgc gggggcgtcc
     1801 ccggcggtaa gggtgcaggc gccgttcttg atgctcaggt agtagttttc cgcgtcctcg
     1861 attttgaact ggaacaccag gtccagcccg gtagcagcgc cgggattgaa gcgggcctgc
     1921 atggtttcga tgactttggc gacagtgctc attatctggt ccttctctcg gcgatagtcg
     1981 tgccgctggc aggcgactgg ctgccggcga gggtaggggg tagcccaaat gcttgtcaac
     2041 ggcggcgcat acgcgcgttc gagtccgtca tggcgagcat ggcggccgcg ctggcgggtg
     2101 gtcgccatcc gttggcgccg gtatgcttga cgggtcgcgg ttcgtgatgc agcgaccatg
     2161 caaacaagga gtcaccgtgg agttttttac cgattacgcc ggttttctgg ccagggcagt
     2221 gaccatcctg gctgccatcc tcgtcgtcct ggccagcatt gccgcgctgc gcacccgcga
     2281 gcgtcgtgcc ggcgggcagc ttgacgtgca caagctcaac gacttctaca agcagctgcg
     2341 cgagcgcctg caggacagcg tgctggacaa ggagcagctg aaggcgctgc gcaaggaaga
     2401 aaccaaggcg gaaaaggccg agaagaaggc cggcagccac aagccgcggg tctacgtgct
     2461 ggacttcgac ggcgacatca aggcttcggc cggcgagcac ctgcgccacg aggtcactgc
     2521 gctgctcagc ctggccaccc cgcaggacga ggtggtggtg cgcctggaga gcggcggcgg
     2581 catggtgcac agctacggcc tggcctcctc gcagctggcg cgcatccgcc aggccggcgt
     2641 gccgctgacg gtgtgcgtgg acaaggtggc ggccagcggc ggctacatga tggcctgcat
     2701 cggcgagaag atcctcgccg cgcccttcgc catcctcggc tcgatcggcg tggtggcgca
     2761 gctgcccaac ctccaccgtc tgctgaagaa gcacgccatc gacttcgagg tgctcaccgc
     2821 cggcgagtac aagcgcaccc tgaccgtgtt cggcgagaat accgataagg gccgggagaa
     2881 gttccaggaa gacttggaca tcacccacga gctgttcaag aacttcgtcg cccgctaccg
     2941 cccgcagctg gccatcgacg aggtggccac cggcgaggtc tggctcggcc aggccgcact
     3001 gggcaagcag ctggtcgacg agctgatgac cagcgacgag tacctgtccg agcgtgccca
     3061 gcacgccgag ctgttccagc tgcactacca cgagaagaag agcctgcagg agcgcgtcgg
     3121 cctggccacc gcccaggtgc tcgacggggt gctgctgcgc tggtggagcc gcctgtcgca
     3181 gccgcgcttc tggcaatgac gatcatccaa gagaggagca cagaatgagc cgtttcaagg
     3241 ccctgctagt caccgaaagc agttcagggc aattcgacca ggccatcgtc gagcgcgagc
     3301 ttgccgagct gcctgcgggc gaggtgctca ttcgtgtgcg ctattcctcg ctcaactaca
     3361 aggacgcgct gtccgccacc ggcagtcgcg gggtgacccg cgcctatccc catacccccg
     3421 gcatcgacgc tgccggcgag gtggtggagt gtgctaccgg tgagttccag ccgggcgatc
     3481 aggtgatcgt caccggctac gatctgggag tgaacagccc aggtggcttt gcccagtaca
     3541 tccgcgtgcc ggccgcctgg gtggtgcgct gcccggcagg catgacgcca cgcgaggcca
     3601 tgctgctcgg caccgccgga ctcaccgccg ggctgtgtgt ggaaaagctg atgcacgtgg
     3661 ggctcgatgc cactgccggc tcggtgctgg tcaccggcgc ttgcggcggg gtgggcagcg
     3721 tcgcggtgct gctgctgacc caccttggct acacggtggc ggcctccacc ggcaagctgc
     3781 agcaggtcga gttcctccat cgcctcgggg cgcagcagat catcgaccgt gcggtactgc
     3841 gcgaggggac cgaaaagccg ctgctgaaga tgcagtgggc cggagcggtg gattccgtgg
     3901 gcggcgatct tctgttcaat gtggtcaagt cgctgcagta cggcgccagc gtggcctgct
     3961 gcggtctgac cgccggggcg gacatggcca cggcctcggt gctgccgttc atcctgcgcg
     4021 gcgtcaacct gctcggcgtc gactcggtgg agctgccgcg cgcggtcaag gccgccatgt
     4081 gggggaagct ggccggaccg tggaagctcg agaccctgga gcagctggct cgggagatca
     4141 ccctggagga gctgcccgag gcgatcagcc agattctcgc cgggcggatg gtcgggcgca
     4201 tgctggtgcg catcgactga gagcctgctc acgatctgct gcgcgtcggc catgcggcgt
     4261 tgaaatcggt ttcggaatgc tcatttgcaa ccagcaaact ccgcttcctc aaccgatttc
     4321 gccttgcctg gctctagctc gcgagatcgt gaacaggctc tgcgcctaat cgacagatgc
     4381 acaacaaaaa gccccgctca tgagcggggc tttttgttga cggggtggaa ctcagcggcg
     4441 gcggaacagc ggcagcggct ggtcgacgcc ggtctggtag gtcaccgaga agtccgacag
     4501 gcccttcagc gcgtcgtacg ggtcgcggtc cgggcgcacg gcgaaggcgt cgaaaccaca
     4561 gcgctgcatg aagaacagct ggtcgcgcag cacgtcgccg atggcgcgga tctcgccctg
     4621 gtagccgtag cgctcgcgca gcaggcgggc gctggagaag tggcggccgt cggtgaaggc
     4681 ggggaagtcg agggcgatga cctggaagtg cttgaggtcg tcgacgatgt cctcgatctg
     4741 ctcgtggctt tgcagccaca cgcccagacc gccgtcgcgc accttgaggg cgtggctgga
     4801 tgccagccac agacccagcg gcacgatcag gtcgtcgctg ttggagatgc cttcgaggct
     4861 ggcgtcgacc ggcagcaggt gccagcgctc gtcgatgact tcgccgttct taatgattct
     4921 ttgcatagac gcgctccttg aaggaatcga tgccgatacg gcggaaggta tcgatgaaac
     4981 gctcttcttc attgcgcttc tccacgtaca cctcgatgac cttggcgatc acgtcggcca
     5041 tgtcgtcctg ggcgaaggac gggccgagga tctggccgag gctggcgttg cggccggctt
     5101 cgccgcccag cgacacctgg tagaactccg cgcccttctt gtcgacgccg aggatgccga
     5161 tgttgccgat gtggtggtga ccacaggcgt tcatgcagcc ggagatgttc aggtcgatct
     5221 cgccgaggtc gaacacgtag tcgaggtcgt cgaagcggcg ctggatggct tcggcgatcg
     5281 ggatcgactt ggcgttggcc agcgagcaga agtcgccgcc cgggcagcag atgatgtcgg
     5341 tcagcaggcc gatgttcggc gtggccaggc ccag
//
LOCUS       DATCUN010000334         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_130475, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000334 DATCUN010000000
VERSION     DATCUN010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130475"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VIO83_12365"
     CDS             <1..486
                     /locus_tag="VIO83_12365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8332105.1"
                     /translation="VLDATAGLGRDAFVLASLGCEVSLIERQPLIAALLEDGLARAAR
                     DPEVAAIVAHMQLLTGNAVALMGAWQGEPPQVIYLDPMFPHRDKSALVKKEMRLFRPL
                     VGDDLDAPALLEAALALASHRVVVKRPRKAPCIDGAKPSYQLEGKSSRYDIYPKKALK
                     G"
     gene            522..1313
                     /locus_tag="VIO83_12370"
     CDS             522..1313
                     /locus_tag="VIO83_12370"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY8332106.1"
                     /translation="MQNVRVALFIDADNSPSRKIEAILAELAVLGVVSIRRAYGNWKS
                     PRLESWVQVLHEHAIQPIQQFDLVKGKNATDMAMAIDAMDVLHGKPVEVFCLVSSDCD
                     FTPLVTRLRAEGKQVIGFGERKSPEPYVNACSRFVYLEEMPVEVTIQPLGTSAVPAEA
                     EAEGLVCAPPAVAVICRRPGHELKGDSKLMSLLRNAVTFAADDDGWAQLSLVGARIAN
                     QASFDPRNYGYARLVDLFTAIDLFEIKRVGRHPLVRYKRRSVTPG"
     gene            complement(1321..>1839)
                     /locus_tag="VIO83_12375"
     CDS             complement(1321..>1839)
                     /locus_tag="VIO83_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEY8332107.1"
                     /translation="AMAWASRLIGGRALLRNWLLVYLGNVVGCLGTVALVLWADVASL
                     GAGAVGETALQIAQRKAELPMGAAFARGILCNALVCLAVWLAMGGHSLTDKFLAILLP
                     ISAFVTLGLEHSIANWFFLPYGLALDGNGSGWLAGAARNLAMVTAGNLVGGTLLVAGV
                     YWLAYLRGSKGA"
BASE COUNT          319 a          632 c          614 g          274 t
ORIGIN      
        1 gtgctggatg ccaccgctgg tctcggccgc gacgccttcg tcctcgccag cctgggttgc
       61 gaggtcagcc tgatcgagcg ccagccgctg atcgccgccc tgctcgagga cggcctggcg
      121 cgcgccgccc gcgaccccga agtggcggcc atcgtggccc atatgcagct gctgaccggc
      181 aacgccgtgg cgctgatggg cgcctggcag ggcgagccac cgcaggtgat ctacctcgac
      241 ccgatgttcc cgcaccgcga caagagcgcg ctggtgaaga aggaaatgcg cctgttccgt
      301 cccttggtgg gcgacgatct cgacgccccg gcgctgctcg aagcggctct ggcgctggcc
      361 agccaccggg tggtggtcaa gcgtccgcgc aaggcgccgt gcatcgacgg cgccaagccg
      421 tcctaccagc tcgaaggcaa gtccagccgc tacgacatct atccgaagaa ggcgctgaag
      481 ggctaggctg aggttactgg gacaaggaag agggccggcc cgtgcagaac gtccgcgtag
      541 cgctgttcat cgatgccgac aactcgccga gccgcaagat cgaggcgatc ctcgccgaac
      601 tggcggtgct cggggtggta tccatccgcc gtgcctacgg caactggaag agcccgcggc
      661 tggagtcctg ggtgcaggtc ctgcacgagc atgcgatcca gccgatccag cagttcgatc
      721 tggtcaaggg caagaacgcc accgacatgg ccatggccat cgacgccatg gacgtgctgc
      781 acggcaagcc ggtcgaggtg ttctgcctag tgtcctcgga ctgcgacttc accccgctgg
      841 tcacccgcct gcgtgccgaa ggcaagcagg tgatcggctt cggcgagcgc aagtcgcccg
      901 agccctacgt caatgcctgc tcgcgcttcg tgtatctgga agaaatgccg gtcgaggtga
      961 ccattcagcc gctgggcacc agcgcggtac cggccgaggc cgaggcagag ggcctggtct
     1021 gtgcaccacc ggcagtggcg gtgatctgcc ggcgtcccgg ccacgaactc aagggcgaca
     1081 gcaagctgat gagcctgctg cgcaatgcgg tgaccttcgc cgccgacgac gacggctggg
     1141 cgcagctcag cctggtcggc gcacggattg ccaaccaggc gtccttcgac ccgcgcaact
     1201 acggctatgc ccggctggtg gacctgttta cggccatcga cctgttcgag atcaagcgcg
     1261 tcggtcgcca cccgctggtg cgctacaagc gccgcagcgt cacgcccgga tagccgtcgc
     1321 tcaggcccct ttgctgccgc gcagataggc cagccagtac acgccggcga ccagcagggt
     1381 gccgccgacc agattgcctg cggtgaccat ggcgaggttt cgcgccgcgc cagccagcca
     1441 cccactgcca ttgccgtcca gcgccaggcc gtagggcagg aagaaccagt tggcgatcga
     1501 gtgttcgagg ccgagggtga cgaatgcact gatcggcagc aggatggcga gaaacttgtc
     1561 ggtcaggctg tgcccgccca tcgccagcca gaccgccagg cacaccaagg cattgcacag
     1621 gatgccgcgt gcaaaggcgg cgcccatggg tagctcggcc ttgcgctggg cgatctgcag
     1681 cgccgtttcc ccgaccgctc cagcgcccag gctggctacg tccgcccaca gcaccagcgc
     1741 aaccgtgccg aggcagccga cgacattgcc caggtagacg agcaaccagt tgcgcagcag
     1801 cgcgcggccg ccgatcaagc ggcttgccca ggccatggc
//
LOCUS       DATCUN010000335         6803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_111940, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000335 DATCUN010000000
VERSION     DATCUN010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111940"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VIO83_12380"
     CDS             <1..241
                     /locus_tag="VIO83_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY8332108.1"
                     /translation="LKRLVKSPYISLPNLLAGRLLVPELIQDAATPETLAATLLPLLD
                     DGHVQTEGFDAIHRALRRGASERAAEAVLQLVERV"
     gene            241..840
                     /gene="rnhB"
                     /locus_tag="VIO83_12385"
     CDS             241..840
                     /gene="rnhB"
                     /locus_tag="VIO83_12385"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY8332109.1"
                     /translation="MQLGLDFAMVEELVAGVDEVGRGPLCGPVVTAAVILDPLRPILG
                     LNDSKKLSEARREKLFDEIREKALAWCIGRAEVEEIDRLNILQATFLAMQRAVEGLHI
                     QPRLALIDGNRCPKLAVPSAPVIQGDGKVPAIAAASILAKVSRDREMAELDALYPGYG
                     MAGHKGYPTAVHLEALRRLGPTPIHRRSFAPVRALLEDG"
     gene            911..4429
                     /gene="dnaE"
                     /locus_tag="VIO83_12390"
     CDS             911..4429
                     /gene="dnaE"
                     /locus_tag="VIO83_12390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY8332110.1"
                     /translation="MTASFVHLRVHTEYSLVDGLVRVKPLVKAAAAAGMPAVAVTDMS
                     NMCSLVKFYKAAMGGGVKPICGADIWLASREEDGPLSRLTLLAMDGKGYRNLTELISR
                     GWTEGQHNDLVIIQRDWVKEAAEGLIALSGAREGEIGLALVNGHPEDAEALLREWSEV
                     FPERFYLEVQRTSRAGDEEYLHAAVELATRCAAPLVATNDVRFLKQDDFEAHETRVCI
                     GEGRTLDDPRRPRTYSDQQYLKTPAEMAELFSDLPEALENTVEIARRCNIEVRLGTHF
                     LPNFPVPEGMTIDDYLRRVSFEGLEERLEVLLPRDTPDYEAKKQVYIDRLEFELGTII
                     QMGFPGYFLIVMDFIKWAKNNGVPVGPGRGSGAGSLVAYVLKITDLDPLAYDLLFERF
                     LNPERVSMPDFDVDFCMDGRDRVIDYVAEAYGRNAVSQIITFGTMAAKAVVRDVARVQ
                     GKSYGLADRLSKMIPFEVGMTLEKAYEMEEPLRDFLSVDEDAREIWDMALKLEGITRG
                     TGKHAGGVVIAPTKLTDFAPIACDDEGGGLVTQFDKDDVEAAGLVKFDFLGLRTLTII
                     KWALETINREQAKKGLEPLNIDFIPLDDKPTYTLLQKAETTAVFQLESRGMKELIKKL
                     KPDCLEDLIALVALFRPGPLQSGMVDDFINRKHGRAEISYPHSDYQYEGLKPVLAPTY
                     GIILYQEQVMQIAQVMAGYTLGGADMLRRAMGKKKPEEMAKQRGGFIEGCANNGIDGD
                     LAGNIFDLVEKFAGYGFNKSHSAAYGLVSYQTAWLKAHFPSPFMAAVLSADMHNTDKV
                     VILIEECRSMKLRIDAPDVNTSEFKFTVNDDGRIVYGLGAVKGVGEGPVEAIVEARAA
                     GPFKDLFDFCARVDLKRINKRTLEALIRCGALDRLGPYYHDEPKAYQATLDLNRAVLM
                     AAMEEAVRAAEQAVRSADSGHMDLFGGVFAEAEADVYANYLKVRELTLKERLRGEKET
                     LGLYLTGHPIDEYEGEVRRFARQRIIDLRPSRETQTVAGLIVNLRVMKNKRGDKMGFV
                     TLDDRSGRIEASLFAEAFAAAQALLQTDALVVVEGEVSMDDFSGGLRLRAKRVMSLEE
                     ARTGLAESLRMKVDGEQLKGERLRWLAELCGQHRGACPITLDYSGSQARALLQFGDQW
                     RIDPNDSLIQTLRDQFGRDNVFLQYR"
     gene            4830..6170
                     /gene="iscB"
                     /locus_tag="VIO83_12395"
     CDS             4830..6170
                     /gene="iscB"
                     /locus_tag="VIO83_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease IscB"
                     /protein_id="HEY8332111.1"
                     /translation="MAVYVLDKSGKPLMPCSEKRARLLLARGRARVHRLAPLVIRLVD
                     RRLTDSAVQPLTLKLDPGSRFTGLALVRQQDRGVSVLSLLELIHRGAAISKAMGQRAS
                     YRRRRRSQNLRHRAPRFDNRNRPEGWLPPSLRHRLDTTLSWVQRLRRWAPITDLTIER
                     VKFDLQKLQNPEISGIEYQQGALQGYEVREYLLEKWGRSCAYCAAESVPLEIEHILAR
                     SNGGSDRVTNLTLACRPCNQRKANQPVEVFLKGKPELLTRIKARSKAPLRDAAAVNAT
                     RNALFDALLQTRLPVATGTGAQTKFNRQRLGIPKTHALDAACVGEVESVASWRRPTLA
                     IKATGRGEYQRTRLTAHGFPRGYLTRQKRHFGFQTGDQVRAQVTAGKKAGAHQGRVAV
                     RQTGSFNIQTPHGVVQGVHHRHCTLIQRADGYAYSQLPFDSAQPRKEAARAGAR"
     gene            6228..>6803
                     /gene="accA"
                     /locus_tag="VIO83_12400"
     CDS             6228..>6803
                     /gene="accA"
                     /locus_tag="VIO83_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEY8332112.1"
                     /translation="MNPNYLDFEQPIADLQAKIEGLRMVGNDNSLNISEEIARLQDKT
                     QSLTQSIFSNLTSWQIARLARHPRRPYTLDYIEHIFEEFDELHGDRHFADDAAIVGGV
                     ARLDGKPVMIIGHQKGREVREKVRRNFGMPRPEGYRKACRLMEMAERFKLPILTFIDT
                     PGAYPGIDAEERGQSEAIAWNLRVMARLKTPI"
BASE COUNT         1251 a         2250 c         2235 g         1067 t
ORIGIN      
        1 cctcaagcgc ctggtgaaaa gcccgtacat ctcgctgccc aacctgctgg ccgggcgcct
       61 gctggtgccc gagctgatcc aggacgcggc gacgcccgag accctggccg ccacgctgtt
      121 gccgctgctg gacgacggtc atgtgcagac cgaaggcttc gacgccatcc accgggcgtt
      181 gcgccggggg gcttccgaac gtgcggccga ggccgtgctg cagctggtgg agcgcgtctg
      241 atgcagctcg gtctggattt cgccatggtg gaagagctgg tcgccggggt cgacgaggtg
      301 gggcgcggtc cgctgtgcgg gccggtggtg accgcggcgg tgatcctcga tccgctgcgg
      361 ccgatcctcg gcctcaacga ttcgaagaag ctcagcgagg cgcgccgcga gaagttgttc
      421 gacgaaatcc gcgaaaaggc actggcctgg tgcatcggtc gtgccgaagt cgaggagatc
      481 gatcggctga atatcctcca ggcgaccttc ctggccatgc agcgggcagt cgaagggctg
      541 cacatccagc cgcgcctggc gctgatcgac ggcaatcgct gcccgaagct ggcggtaccc
      601 agcgcgccgg tgatccaggg cgacggcaag gtgccggcga tcgccgccgc ctcgatcctg
      661 gccaaggtca gccgcgaccg cgaaatggcc gagctggatg ccctctaccc gggctacggc
      721 atggccgggc acaagggcta tcccaccgca gtgcacctcg aggcactgcg ccgcctcggg
      781 ccgaccccca ttcatcggcg ctccttcgct ccggtgcggg cattgctgga agacggctga
      841 gtcaaggctg tggtttgccc caaggcccgg tacaattccg ggccttgtcg tttctgcctc
      901 acggattgcc atgactgcct ccttcgtcca tctgcgcgtg cacaccgaat attccctggt
      961 cgatggcctg gtgcgggtca aaccgctggt caaggcggcc gcggcggcgg gcatgccggc
     1021 ggtggcggtc accgacatga gcaacatgtg ctcgctggtc aagttctaca aggcggcgat
     1081 gggcggcgga gtcaagccga tctgcggcgc ggacatctgg ctggccagcc gcgaggaaga
     1141 cggtccgctc agccgcctga ctctgctggc gatggacggc aagggctatc gcaacctcac
     1201 cgagctgatc tcgcgcggct ggaccgaagg ccagcacaat gacctggtga tcatccagcg
     1261 cgactgggtg aaggaagcgg ccgagggctt gatcgccttg tcgggcgccc gcgagggcga
     1321 gatcggtctg gccctggtca atggccatcc cgaggacgcc gaggcactgc tgcgcgagtg
     1381 gtcggaggtg tttcccgagc gcttctacct cgaggtgcag cgcaccagcc gcgccggcga
     1441 cgaagagtac ctgcatgccg ctgtcgagct ggccacgcgc tgcgctgcgc cgctggtggc
     1501 gaccaacgac gtgcgcttcc tcaagcagga cgacttcgag gcccacgaga cccgcgtgtg
     1561 catcggcgag ggacgcaccc tcgacgatcc gcgccggccg cgcacctact cggaccagca
     1621 gtacctgaag accccggcag agatggctga gctgttcagc gacctccccg aggcgctgga
     1681 aaacaccgtc gagatcgccc gccgctgcaa catcgaggtg cgcctgggca cccacttcct
     1741 gcccaacttc ccggtgcccg aaggcatgac catcgacgac tacctgcgcc gggtgtcgtt
     1801 cgagggcctc gaggagcgcc tggaggttct gttgcccagg gacacgccgg actacgaggc
     1861 gaagaagcag gtctacatcg accgtctgga gttcgagctg ggtaccatca tccagatggg
     1921 tttccccggc tacttcctga tcgtgatgga cttcatcaag tgggcgaaga acaacggcgt
     1981 gccggtcggt cccggccggg gctcgggtgc cggctcgctg gtggcctacg tgctgaagat
     2041 caccgacctc gacccgctgg cctacgacct gctgttcgaa cgcttcctca accccgagcg
     2101 ggtgtccatg cccgacttcg acgtcgactt ctgcatggac ggccgcgacc gggtgatcga
     2161 ctacgtggcc gaggcctacg ggcgcaacgc ggtcagccag atcatcacct tcggcaccat
     2221 ggcggccaag gcggtggtgc gcgacgtggc gcgggtgcag ggcaagtcct acggcctggc
     2281 cgaccgcctg tcgaagatga ttcccttcga agtcggcatg accctggaaa aagcctacga
     2341 gatggaggag ccgctgcgcg acttcctgtc cgtcgacgag gatgccaggg aaatctggga
     2401 catggccctc aagctcgagg gtatcacccg cggcaccggc aagcacgccg gcggcgtggt
     2461 gatcgcgccg accaagctca ccgattttgc gccgatcgcc tgcgacgacg agggtggcgg
     2521 cctggtgacc cagttcgaca aggacgacgt cgaggcggcc ggcctggtca agttcgactt
     2581 cctcggcctg cgcaccctga ccatcatcaa gtgggcgctg gagacgatca accgcgaaca
     2641 ggcgaagaag ggcctggagc cgctgaacat cgacttcatc ccgctggacg acaagccgac
     2701 ctacacgctg ctgcagaagg ccgagaccac cgcggtattc cagctcgaat cgcgcggcat
     2761 gaaggagctg atcaagaagc tcaagccgga ctgcctggaa gacctcatcg ccctggtggc
     2821 gctgttccgt cccggcccgc tgcagtcggg catggtggat gacttcatca accgcaagca
     2881 cggccgtgcg gagatatcct atccgcactc cgactatcag tacgagggcc tcaagccggt
     2941 actggcgccg acctacggca tcatcctgta ccaggaacag gtgatgcaga tcgcccaggt
     3001 gatggctggc tacaccctcg gcggcgcgga catgctgcgt cgggcgatgg gcaagaagaa
     3061 gcccgaggaa atggccaagc agcgcggcgg cttcatcgag ggctgcgcca acaacggcat
     3121 cgatggcgat ctggcgggca acatcttcga cctggtggag aagttcgccg gctacggctt
     3181 caacaagtcg cactcggccg cctacggcct ggtctcctat cagaccgcct ggctgaaggc
     3241 ccacttcccg tcgccgttca tggccgcggt gctgtcggcg gacatgcaca acaccgataa
     3301 ggtggtgatc ctcatcgagg agtgccgcag catgaagctg cgcatcgacg cccccgacgt
     3361 caacacctcc gagttcaagt tcaccgtcaa cgacgacggg cgcatcgtct acggcctggg
     3421 tgcggtcaag ggcgtcggcg aggggccggt ggaggccatc gtcgaggcgc gcgccgcggg
     3481 gccgttcaag gacctgttcg acttctgcgc gcgcgtcgac ctcaaacgca tcaacaagcg
     3541 caccctggag gcgctgatcc gctgcggcgc gctggatcgc ctcggcccgt actaccacga
     3601 cgagcccaag gcctatcagg ccaccctgga cctcaatcgc gccgtgctga tggcggcgat
     3661 ggaggaagcg gtgcgcgcgg cggagcaggc ggtgcgcagt gccgacagcg ggcacatgga
     3721 cctgttcggc ggggtgttcg ccgaggccga ggcggacgtc tacgccaact acctcaaggt
     3781 ccgcgagctg accctcaagg agcgcctgcg cggcgagaag gaaacccttg gcctgtacct
     3841 cactggccat ccgatcgacg agtacgaggg cgaggtgcgc cgctttgccc gccagcgcat
     3901 catcgacctg cgtccgtcgc gcgagactca gacggtggcc ggcctgatcg tcaacctgcg
     3961 ggtgatgaag aacaagcgcg gcgacaagat gggcttcgtc accctcgacg accgctccgg
     4021 acgcatcgag gcctcgctgt tcgccgaggc gttcgccgcc gcccaggcgc tgctgcagac
     4081 cgatgcgctg gtggtggtgg aaggcgaagt cagcatggac gacttctccg gcggcctgcg
     4141 cctgcgcgcc aagcgggtga tgagcctgga ggaggcgcgc accggcctgg ccgagagcct
     4201 gcgcatgaag gtcgacggcg agcagctcaa gggtgagcgc ctgcgctggc tggccgagct
     4261 gtgcggccag caccgcggcg cctgcccgat caccctcgac tacagtggta gccaggcgcg
     4321 cgccctgctg cagttcggcg accaatggcg aatcgacccg aacgacagcc tgattcagac
     4381 tttgcgtgac cagttcgggc gcgacaacgt cttcctgcaa taccgttaag ggcgggtctc
     4441 gcaacccgcc tggctcgacc cgcgacgccc gatcccgtaa agtcgcgtgt ttgttttccg
     4501 agccgacccg caacaagtta tccacggatg cctgcgtcaa ctacccctcc ctgaagtaga
     4561 ggggcttgtg gggtaaccca taagccgggt tggccaggga gagccgtatc caatcggctc
     4621 cgtttgcaac aggtcgaaca gacccacttc gggatgcttc ctcagtcccg aactctggaa
     4681 ggctgggatc atgctggcgc aaggtaaaac gccgaaggtc ccggtcgccg cgacagcggg
     4741 agccggttgc agacattccc gaggggagcg agggtctcag ccctccgtca ccaggcccgt
     4801 aagggcagag atcgaaaagg aggatcgcca tggcggtcta cgtgttggac aagagcggaa
     4861 agccgctgat gccgtgcagc gagaaacgtg cgcgcctgct gttggcgcgg ggtcgcgccc
     4921 gtgttcaccg gctggcgccg ttggtgatcc gcctggtgga tcgccggctg accgatagcg
     4981 ccgtgcaacc gctgacgctc aagctcgatc cgggcagccg gtttaccggc ctggccctgg
     5041 tgcgccagca ggatcgcggg gtgtcggtgt tgtcactgct agagctaatt caccgtggcg
     5101 ccgctatcag caaagccatg gggcagcgcg ccagctaccg tcgccgccgc cgcagtcaga
     5161 acctgcgcca tcgcgccccg cgcttcgata atcgcaaccg gccggaaggc tggctgccgc
     5221 cgagtctgcg gcatcggctg gacaccacgt tgagctgggt gcagcggctg cgccgctggg
     5281 ccccgatcac cgaccttacc atcgagcgcg taaagttcga cctacagaaa ctgcaaaatc
     5341 cggaaatcag cggcatcgag taccagcaag gcgcgttgca gggctacgag gtgcgcgaat
     5401 acctgctgga gaaatggggg cgtagctgcg cctattgcgc tgcggaaagc gtcccgctgg
     5461 aaattgagca catcctggcc cggagcaatg gtggcagcga ccgggtgacc aacctgaccc
     5521 tggcgtgtcg accgtgcaac cagcgcaagg ccaaccagcc ggtcgaggtg ttcctgaagg
     5581 gcaaacccga actgctgacc cggatcaagg cccggtccaa ggctccgctt cgagatgccg
     5641 cagccgtcaa cgcgacccgc aacgccctgt tcgacgcctt actgcaaacc cgcctgccag
     5701 tggccaccgg caccggggcg cagaccaagt tcaaccgcca acgcctgggt atcccgaaaa
     5761 cccatgcgct cgatgccgcc tgcgtcggcg aggtggaatc agtagccagc tggcgaagac
     5821 ccacgctggc gatcaaggct accggccgcg gcgagtacca gcggacccgc ctgaccgccc
     5881 acggtttccc tcgcggctac ctgacccggc agaaacgcca cttcggattc cagaccgggg
     5941 atcaggtacg agcccaggtg acggccggca aaaaggccgg cgcccatcag ggccgcgtgg
     6001 ctgtgcgcca gaccggcagc ttcaacatcc agacacctca tggcgtcgtg caaggcgttc
     6061 accaccgcca ctgcaccctg atccagcgag ccgatggcta cgcctattcc caactgccgt
     6121 ttgatagcgc ccaaccccgg aaggaggcag cgagagcagg ggcacgctga gcgtgccgcg
     6181 ctatccctcc ccgcactgaa gtacggggtt tctcgcgcaa aagatagatg aacccgaatt
     6241 atctggattt cgaacagccg atcgccgacc tgcaagccaa gatcgaaggc ctgcgcatgg
     6301 tgggcaacga caactcgttg aacatcagcg aggagattgc ccgcctgcag gacaagacgc
     6361 agtcgctgac ccagagcatt ttcagcaatc tgaccagctg gcagatcgcg cgcctggccc
     6421 gtcatccgcg tcgtccctac accctcgact acatcgagca catcttcgaa gagttcgacg
     6481 aactgcacgg cgaccgccac tttgccgacg atgccgccat cgtcggtggc gtcgcgcgcc
     6541 tggacggcaa gccggtgatg atcatcggcc accagaaggg ccgcgaggtg cgcgagaagg
     6601 tgcgtcgcaa cttcggcatg ccgcgtccgg aaggctaccg caaggcctgc cgcctgatgg
     6661 aaatggccga gcgcttcaag ctgccgatcc tgaccttcat cgacaccccc ggcgcctacc
     6721 cgggcatcga cgccgaagag cgtggccaga gcgaggccat cgcctggaac ctgcgcgtga
     6781 tggcgcgcct gaagaccccg atc
//
LOCUS       DATCUN010000336         6768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_92402, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000336 DATCUN010000000
VERSION     DATCUN010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92402"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..861)
                     /locus_tag="VIO83_12405"
     CDS             complement(1..861)
                     /locus_tag="VIO83_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY8332113.1"
                     /translation="MSAGLAITITLLVFVVITIAKGVRLVSQGEEWIVERLGKYHSTL
                     RPGLNILIPYLDNVAYKLVTKDIILDVKEQEVITRDNAVILANAIAFVKVTDPVKAVY
                     GVTDFSEAIRNLIMTTLRSIVGEMELDAALSSRDTIKARLRESIADEAVDWGLTVKSV
                     EIQDIKPSESMQRAMELQAAAERERKAAVTKAEGAKQAAILEAEARLESAKRDANAQV
                     MLAEASAEAIRRVTSAVGNEPGPMMYLLGEKYIASLEKLAASDNAKVVVMPADLQETL
                     RGVLGKLGGR"
     gene            complement(900..1343)
                     /locus_tag="VIO83_12410"
     CDS             complement(900..1343)
                     /locus_tag="VIO83_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003257542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY8332114.1"
                     /translation="MIIEWWYWAIGGILLILLELAIPAFFVIWFGLGALLVAGVLLVA
                     GALSLTAQLLLWILASLAMVGLWLRVFRPSRHRTLVGTADGEVIGEVGLLVGAVAPYQ
                     RGKVRFQRPILGSEEWACMAESNITAGERVRVVSIEGSYLKVARA"
     gene            complement(1446..1742)
                     /locus_tag="VIO83_12415"
     CDS             complement(1446..1742)
                     /locus_tag="VIO83_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY8332115.1"
                     /translation="MDVSKTRTSFYRRLYVAWLIDSATATSVPALMAATGMPRRTAQD
                     TVAALAELDIDCQFQQDDGERHNAGHYAIRAWGAIDKRWVGAHLAQIKAVLGYP"
     gene            complement(1756..3123)
                     /gene="nhaD"
                     /locus_tag="VIO83_12420"
     CDS             complement(1756..3123)
                     /gene="nhaD"
                     /locus_tag="VIO83_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HEY8332116.1"
                     /translation="MYAWMALIFVVGYLCIALEHPLKIDKAASAILTAVLCWTVLVLG
                     ADSILPAAEAAVASAAGDHGVVGELRHHLGEISEILFFLLGAMTIVELVDAHEGFRVI
                     TDRIQTRKRVQLLWLIGFITFFLSAALDNLTTTIVMISLLRKLIGDPRERWFYAGIVV
                     IAANAGGAWSPIGDVTTTMLWIGNQITTGGIISSLFLPSLVCLLVPLVVLSFILSGET
                     ERPQNGLTEEGRRDPTTAFERQLVLALGLGSLLFVPVFKTVTHLPPYMGILFGLGVLW
                     VATEVIHRGKNDEDKHPLSVIGVLRKIDTPSILFFLGILLAVSSLATAGHLTQVAGAL
                     KDSLGNVYAINIAIGLLSAVVDNVPLVAGAMKMYPLVTPAAAAAGDTGWLQHFIADGT
                     FWEMLAYCAGTGGSCLIIGSAAGVAAMGMERINFLWYLKYISGLALLGYLGGAATYIA
                     LRTLI"
     gene            complement(3291..6137)
                     /locus_tag="VIO83_12425"
     CDS             complement(3291..6137)
                     /locus_tag="VIO83_12425"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY8332117.1"
                     /translation="MDKTYQPHEIESRWYAEWEAKNYFAPQGSGESYTIALPPPNVTG
                     SLHMGHGFNNAIMDALIRFRRMQGRNTLWQPGTDHAGIATQMVVERQLAAQNLSRHDL
                     GREKFLEKVWEWKEESGGTITRQIRRLGSSVDWSRERFTMDEGLSEAVKEAFVRLHED
                     GLIYRGKRLVNWDTKLHTAISDLEVENHDEKGSLWNLRYPLADGAKTAEGQDYLIVAT
                     TRPETMLGDSAVAVNPQDPRYQALIGKFVELPLVGRRIPIIGDDYCDPEFGTGCVKIT
                     PAHDFNDYEVGKRHHLPLINILDKDAAILATAQVFNLDGSVNAAFDARLPAQFAGLDR
                     FEARKQIVAAFDAAGLLEKIDDHALKVPKGDRSGTIIEPWLTDQWYVSTKPLAEKAIA
                     AVEDGQIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRDEAE
                     VRAKHNLGDIALRQDDDVLDTWFSSGLWTFSTLGWPQQTEFLKTFHPTDVLVTGFDII
                     FFWVARMIMMSLHLTGQIPFKTVYVHGLVRDGQGHKMSKSKGNVLDPLDIVDGIDLET
                     LVTKRTSGMMQPKLAEKIAKQTRAEFPDGIASYGTDALRFTFCSLASTGRDIKFDMGR
                     VEGYRNFCNKLWNATNFVLENTDGKDCGAHDEAAAGAPVELSPVDRWIISALQRCEQD
                     VTRHLDQFRFDLAAQSLYEFVWDEYCAWYLELVKPVLWDEQASVERQRGTRRTLVRVL
                     EVILRLAHPFMPFITEEIWQRIKALAGKSGDTLMLQPWPEANESRIDAAAEGDIEWVK
                     QLMLGVRQIRGEMKISMAKRIDVVLANASAEDQRRLADFEPLLAKLAKFESVRVLAAG
                     EEAPLAATALVGEMEVLVPMAGLIDKDAELARLDKELQRLQGEVQRVGGKLANEGFVA
                     KAPEAVLDKERAKLAEAEQALAKLAEQRAKIAAL"
     gene            complement(6336..>6768)
                     /locus_tag="VIO83_12430"
     CDS             complement(6336..>6768)
                     /locus_tag="VIO83_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY8332118.1"
                     /translation="DQLKGRWTLLFFGYTFCPDICPTTLAELRQLKSQLSPEALARLQ
                     VTMVSIDPHRDTPAQLKQYLGYYKAGYSGLTGELADIQALANAVSIPFIPGDTSREHY
                     TVDHSGNLAVIGPDGRQRGFIRAPLNVAKLAVQLPALLKQD"
BASE COUNT         1044 a         2134 c         2403 g         1187 t
ORIGIN      
        1 ttagcgcccg cccagcttgc cgagcacgcc gcgcagggtt tcctgcaggt cggcgggcat
       61 caccaccacc ttggcgttgt cgcttgccgc gagcttttcc agcgaggcga tgtacttctc
      121 gccgagcagg tacatcatcg gcccgggctc gttgccgacc gcgctggtca cccggcggat
      181 cgcttcggcc gaggcttcgg ccagcatcac ctgggcgttg gcgtcgcgct tggccgactc
      241 caggcgggcc tcggcttcga ggatcgccgc ctgcttggcg ccttcggcct tggtcaccgc
      301 ggccttgcgc tcgcgctcgg cggcggcctg cagctccatg gcccgctgca tggattccga
      361 cggcttgatg tcctggatct ccaccgactt caccgtcagg ccccagtcca ccgcctcgtc
      421 ggcgatgctt tcgcgcaggc gcgccttgat ggtgtcgcgc gaggacagcg ccgcgtccag
      481 ctccatttcg ccgacgatcg agcgcagggt ggtcatgatc aggttgcgga tcgcctcgga
      541 gaagtcggtg acgccgtaca ccgccttgac cgggtcggtg accttcacga aggcgatggc
      601 gttggcgagg atcaccgcgt tgtcgcgggt gatcacttcc tgttccttga cgtcgaggat
      661 gatgtccttg gtcaccagct tgtaggcgac gttgtccagg taggggatca ggatgttcag
      721 gccgggacgc agggtgctgt ggtacttgcc gagccgctcg acgatccact cctcgccctg
      781 ggagaccagg cgcacgccct tggcgatggt gatgaccacg aacaccagca gggtgatggt
      841 gatggctagt cctgcactca tgtacttctc cttgaaatca gtccttgaaa ccgggttgat
      901 caggcgcggg ctaccttgag gtagctgcct tcgatggata ccacgcgaac ccgctccccg
      961 gcggtgatgt tcgactcggc catgcaggcc cactcctcgg agccgaggat cggccgctgg
     1021 aagcgcacct tgccgcgctg gtagggcgcg acggcgccga ccagcaggcc gacctcgccg
     1081 atcacctcgc cgtcggcggt gccgaccagg gtgcggtgcc gactcgggcg gaacacgcgc
     1141 agccacaggc cgaccatcgc cagcgaggcg aggatccaca gcagcagttg tgcggtcagc
     1201 gacagcgcgc cggcgaccag caggacgccg gccaccagca gggcgccgag gccgaaccag
     1261 atcacgaaga acgccgggat cgccagctcc agcaggatca gcaggatgcc gccgattgcc
     1321 caataccacc actcgataat catcgcgcct ccatttgcag cgaaaattcc cgctccggtg
     1381 gcggagcggg ctggagcaag gatatcgccg aaccgctgtc cgggtcagtg accgtctgca
     1441 ggcgctcagg gatagccgag cacggccttg atctgcgcca gatgcgcgcc gacccagcgc
     1501 ttgtcgatgg cgccccaagc gcggattgcg tagtggccgg cgttgtgccg ctcgccgtcg
     1561 tcctgctgga actggcagtc gatgtccagc tcggccagcg ccgccacggt gtcctgggcg
     1621 gtgcgccgcg gcatgccggt ggcggccatc agcgccggca cgctggtggc ggtggcgctg
     1681 tcgatcagcc aggccacgta caggcggcgg tagaagctgg tgcgggtctt gctcacgtcc
     1741 atgtgcggtg ctccgtcaga tcagggtgcg cagggcgatg taggtggcgg cgccgccgag
     1801 gtagccgagc agggccaggc cgctgatgta cttgaggtac cagaggaagt tgatccgctc
     1861 catgcccatg gcggcgacgc cggccgccga gccgatgatc aggcagctgc cgccggtgcc
     1921 ggcgcagtag gcgagcatct cccagaaggt gccgtcggcg atgaagtgtt gcagccagcc
     1981 ggtgtcgccg gcggccgcgg cagccggcgt gaccagcggg tacatcttca tcgcgccggc
     2041 caccagcggc acgttgtcga ccacggcgga gagcaggccg atggcgatgt tgatggcgta
     2101 gacgttgccg aggctgtcct tcagtgcgcc ggcgacctgg gtcaggtggc cggcggtggc
     2161 caggctggac accgccagca ggatgccgag gaagaacagg atgctcgggg tgtcgatctt
     2221 gcgcagtacg ccgatcaccg acagcgggtg cttgtcctcg tcgttcttgc cgcggtggat
     2281 cacctcggta gctacccaga gcacgcccag gccgaacagg atgcccatgt acggcggcag
     2341 gtgggtgacg gtcttgaaca ccggcacgaa cagcagcgag ccgaggccca gcgccagcac
     2401 cagctggcgc tcgaaggccg tggtcgggtc gcggcggcct tcttcggtga ggccgttctg
     2461 cgggcgctcg gtttcgccgc tgaggatgaa gctgagcacc accagcggca ccagcaggca
     2521 caccaggctg ggcaggaaca ggctgctgat gatgccgccg gtggtgatct ggttgccgat
     2581 ccacagcatg gtggtggtga cgtcgccgat cggcgaccag gcgccgccgg cgttggcggc
     2641 gatcaccacg atgccggcgt agaaccagcg ctcgcgcgga tcgccgatca gcttgcgcag
     2701 cagcgagatc atcacgatgg tggtggtcag gttgtccagc gcggcggaga ggaagaaggt
     2761 gatgaagccg atcagccaca gcaactgcac gcgcttgcgg gtctggatgc ggtcggtgat
     2821 gacgcgaaag ccctcgtggg cgtcgaccag ctcgacgatg gtcatggcgc cgagcaggaa
     2881 gaacaggatt tcggagattt caccgaggtg gtggcgcagc tcgccgacca cgccatgatc
     2941 gccggcggca ctggctacgg cggcctccgc ggccggcagg atgctgtcgg caccgaggac
     3001 cagcacggtc cagcacagca cggcggtgag aatggccgag gcggccttgt cgattttcag
     3061 cgggtgttcg agggcgatgc agaggtaacc gacgacaaag atcagggcca tccaggcgta
     3121 catgggcggg cttccggtgg gtcagtgggc ggattgttgt tttcggatgt tgcaactggc
     3181 gggcgagcat gcctgatgag gggcggcgct gaaaagcgcc gctctgtcgc agcgcacaga
     3241 aaaaggcccg ctaccttgcg gtagcgggcc ttgcgcctga cagcggcggt ttagagcgcg
     3301 gcgatcttgg cgcgctgctc ggccagcttg gccagcgcct gctcggcctc ggccagcttg
     3361 gcgcgttcct tgtccagcac ggcttccggc gccttggcga cgaagccttc gttggccagc
     3421 ttgccgccga cgcgctgcac ctcgccttgc aggcgctgca gttccttgtc gaggcgcgcc
     3481 agctcggcgt ccttgtcgat cagcccggcc atcggcacca gcacttccat ctcgccgacc
     3541 agcgcggtgg cggccagcgg cgcttcctcg ccggcggcca gcacgcgcac cgattcgaac
     3601 ttggccagct tggccagcag cggctcgaag tcggccaggc ggcgctggtc ctcggcactg
     3661 gcgttggcca gcaccacgtc gatgcgcttg gccatggaga tcttcatttc gccacggatc
     3721 tgccgcacgc cgagcatcag ctgcttgacc cactcgatgt cgccttcggc ggccgcgtcg
     3781 atgcggctct cgttggcttc cggccagggc tgcagcatca gggtgtcacc ggacttgccg
     3841 gccagcgcct tgatgcgctg ccagatttcc tcggtgatga acggcatgaa cggatgggcc
     3901 aggcgcagga tgacctccag cacgcgcacc agggtgcggc gggtgccgcg ctggcgctcg
     3961 acggaagcct gctcgtccca cagcaccggc ttgaccagct ccagatacca ggcgcagtac
     4021 tcgtcccaga cgaactcgta gagcgactgg gcggccaggt cgaagcggaa ctggtcgagg
     4081 tgacgggtga cgtcctgctc gcagcgctgc agcgcggaga tgatccagcg gtccaccggc
     4141 gacagctcga ccggagcgcc ggccgccgcc tcgtcatggg cgccgcagtc cttgccgtcg
     4201 gtgttctcca gcacgaagtt ggtggcgttc cacagcttgt tgcagaagtt gcggtagccc
     4261 tcgacgcggc ccatgtcgaa cttgatgtcg cggccggtgg aggccagcga gcagaaggtg
     4321 aagcgcagcg cgtcggtgcc gtagctggcg atgccgtcgg ggaactcggc gcgggtctgc
     4381 ttggcgatct tctcggccag cttgggctgc atcatgccgc tggtgcgctt ggtcaccagg
     4441 gtttccaggt cgatgccgtc gacgatgtcc aaaggatcca gcacgttgcc cttggacttg
     4501 gacatcttgt ggccctggcc gtcgcgcacc agaccgtgga cgtagacggt cttgaacggg
     4561 atctgcccgg tcaggtgcag gctcatcatg atcatccggg cgacccagaa gaagatgatg
     4621 tcgaagccgg tgaccagcac gtcggtgggg tggaaggtct tcaggaactc ggtctgctgc
     4681 ggccagccga gggtggagaa ggtccacagg ccggagctga accaggtgtc cagcacgtcg
     4741 tcgtcctggc gcagggcgat gtcgccgagg ttgtgcttgg cgcgcacttc ggcctcgtcg
     4801 cggccgacgt agacgttgcc ggcctcgtcg taccaggccg ggatgcggtg gccccaccac
     4861 agctggcggc tgatgcacca gtcctggatg tcgcgcatcc agctgaagta catgttctcg
     4921 tactgcttgg gtacgaactg gatctggccg tcttccacgg cggcgatggc cttctcggcc
     4981 agcggcttgg tggagacgta ccactggtcg gtcagccacg gctcgatgat ggtgccggag
     5041 cggtcgccct tgggcacctt gagggcgtgg tcgtcgatct tctccagcag gccggcggcg
     5101 tcgaaggcgg cgacgatctg cttgcgcgcc tcgaagcggt cgaggccggc gaactgcgcc
     5161 ggcaggcggg cgtcgaacgc ggcgttgacg ctgccgtcga ggttgaacac ctgggcggtg
     5221 gcgaggatcg ccgcgtcctt gtcgaggatg ttgatcagcg gcaggtggtg gcgcttgccg
     5281 acttcatagt cgttgaagtc gtgggccggg gtgatcttca cgcagccggt gccgaactcg
     5341 ggatcgcagt agtcgtcgcc gatgatcggg atgcgccggc cgaccagtgg cagctcgacg
     5401 aacttgccga tcagcgcctg gtagcgcgga tcttgcgggt tcaccgccac ggccgagtcg
     5461 ccgagcatgg tttccggacg ggtggtggct acgatcaggt agtcctggcc ctcggcggtc
     5521 ttggcgccgt cggccagcgg gtagcgcagg ttccacagcg aacccttctc gtcgtggttc
     5581 tccacttcga ggtcggagat cgccgtgtgc agcttggtgt cccagttgac caggcgcttg
     5641 ccgcggtaga tcaggccgtc ctcgtgcaga cgcacaaagg cttccttgac cgcctcggag
     5701 aggccctcgt ccatggtgaa gcgctcgcgc gaccagtcca ccgaggagcc gagacggcgg
     5761 atctgccggg tgatggtgcc gccggactct tccttccact cccagacctt ctcgaggaac
     5821 ttctcgcggc cgaggtcgtg gcgcgacagg ttctgcgcgg ccagctggcg ctccaccacc
     5881 atctgggtgg cgatgccggc gtggtcggtg cccggctgcc acagggtgtt gcggccctgc
     5941 atgcggcgga agcggatcag cgcgtccatg atcgcgttgt tgaagccgtg gcccatgtgc
     6001 aggctgccgg tgacgttggg cggcggcagg gcgatggtgt aggactcgcc ggaaccttgc
     6061 ggcgcgaagt agttcttcgc ctcccactcg gcgtaccagc gggattcgat ttcgtggggt
     6121 tggtaggtct tgtccatgcg cggcgggacc cttgcggcag ctgatcggaa aaccgcgaag
     6181 tataacgggg atcgggcgca gggcgtaggg catgctcgat ggggcggacc tgtgtagggt
     6241 ggatggccac cccgtggaaa actcgcgcag cgattttccg ccatgagccg cacactggtg
     6301 gacaaggctg cgccgttgtc caccctaccc catcctcagt cctgcttgag cagtgccggc
     6361 agctgcaccg ccagcttggc gacgttcagc ggcgcgcgga tgaagccgcg ctgacggccg
     6421 tccgggccga tcacggcgag attgccgctg tggtcgacgg tgtagtgctc gcggctggtg
     6481 tcgccgggaa tgaagggaat gctcaccgcg ttggccagcg cctggatgtc cgccagctcg
     6541 ccggtcagcc cgctgtagcc ggccttgtag tagccgaggt actgcttgag ctgcgcgggc
     6601 gtatcgcgat gggggtcgat gctgaccatg gtcacctgca ggcgggccag cgcctccggc
     6661 gacagctggc tcttgagctg gcgcagctcg gccagggtgg tcgggcagat gtccgggcag
     6721 aaggtgtagc cgaagaacag cagggtccaa cggcccttga gctggtcc
//
LOCUS       DATCUN010000337         5684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_56256, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000337 DATCUN010000000
VERSION     DATCUN010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56256"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VIO83_12435"
     CDS             complement(<1..229)
                     /locus_tag="VIO83_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HEY8332119.1"
                     /translation="MSVTQQTLEQLAAALRGAEASGEAVEPLRGLIGEDNGAAAYAIQ
                     RLNIAHGVANGRRVVGRKIGLTNPKVQAQLGV"
     gene            complement(595..1458)
                     /locus_tag="VIO83_12440"
     CDS             complement(595..1458)
                     /locus_tag="VIO83_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8332120.1"
                     /translation="MTSITLSEASTSQFVRIQDGELDLNIHYNDCGAGAETVVMLHGS
                     GPGASGWANFNRNIEPLVAAGYRVILMDCPGWSKSDSIVCTGSRSDLNARVLKGLVDA
                     LQLDRVHILGNSMGGHSAVAFALANPERIGKLVLMGGGTGGASLFTPMPTEGIKLLQG
                     LYREPTIDNLKKMMNVFVFDSSDLTEELFQARLDNMLNRRDHLENFVKSLAANPKQFP
                     DFSPRLAEIKAQTLVIWGSNDRFVPMDAGLRLLAGLPNAELHVFNRCGHWAQWEHADK
                     FNRMVLDFLSH"
     gene            complement(1476..2033)
                     /locus_tag="VIO83_12445"
     CDS             complement(1476..2033)
                     /locus_tag="VIO83_12445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY8332121.1"
                     /translation="MSKEINQLLDWIPNADLAALPVVSADDHRVGMRNLAAAVTIITA
                     RDGDTHLGLTATAVCSVTAEPPRLVVFVNKKVAASAAILNSGALCVNVLSGEQEHEAK
                     VFAGMVEGVHGEARFQHGQWHDLASGVPVLDGAVANFDCRVVKVFDESTHHAFLCEVL
                     ATRGQQGGEPLLYLNGAFRRLAPQA"
     gene            complement(2104..3300)
                     /locus_tag="VIO83_12450"
     CDS             complement(2104..3300)
                     /locus_tag="VIO83_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8332122.1"
                     /translation="MSHTDMQIPSEAEIVARAEAMIPWLREKADATEKARMVSRETIQ
                     AFQEAGFFKILQPKRWGGYEMHPNVLNKVLMELARGCPSSAWNVMVLGVHPFEIGLLD
                     PRCGDELWGEDNTRLVSSSYAPFGTVTRVDGGYQLNGEWLTSSGCDHAAGGAFLGGRV
                     VNDAGQMEFRSFWVQASDFEIVDDWHVVGLAGTGSKKLLIKEVFVPTYRSHVIGDYDQ
                     HSHGHVNNLYKVPFFFVFYAAVSSVIIGMARGMVDLYVDHMKPRQNLNQAVGAAVNDP
                     FIKSRLGEAYAKILGATTRVLHNTEEAWSYASQGQLVPLDVRVRHFATNQFTGGECFD
                     AAHMIFKKTATRGVWLSSPMQRQMRDILVGANHITQNQDNIGDLLGGQLLGNPLPAAN
                     PFGVKA"
     gene            complement(3371..4309)
                     /locus_tag="VIO83_12455"
     CDS             complement(3371..4309)
                     /locus_tag="VIO83_12455"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008047644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HEY8332123.1"
                     /translation="MTVKLKCLSHTPLRGLNDPGAAVVAEVDAALAKARAEVEAFDPE
                     LIVIFAPDHYNGLFYDLMPPFVIATAAESVGDYLTQPGPLSVAKDLALELTRHVLDSD
                     VDIALSHRLQVDHGCTQTLEELTGSLDRYPVIPIIINSVAPPFAPYRRVRKLGEAVGR
                     FIAKLDKRVLILGTGGLSHEPPVPLLADAPEAISEFLIAGRNPTPEARAARQARTIAA
                     GKIYGTAESLQTPLNADWDLAFIDLLVQGRLDEIDEFKIDEISKAAGRSAHEVRTWVA
                     AFAALAATGAYSARQDYYRAINEWIAGYGVVSAEQN"
     gene            4796..5611
                     /locus_tag="VIO83_12460"
     CDS             4796..5611
                     /locus_tag="VIO83_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEY8332124.1"
                     /translation="MSGTRETEYKTVRGLTRGLQILNALNRVDGGASPARLAELTGLH
                     RTTVRRLLETLQDEGYVRRSESDDSFRLNLKVRELSEGFRDEQWISELGAPLLAELLL
                     EVHWPTDLCTLDVDAMVVRETTHRFSRLSFHRSMVGRRLPMLITATGLAYLAFCPEAE
                     RERIVELLAKRDDEDCRLARNPVALDNLLRRTRHRGYGENFGSWNSEERTASIAVPIC
                     GEQRVYGCLNLVYVLKAMTIEQAAQRYLPALQRVAATIEQGIREREQAGPVLA"
BASE COUNT          912 a         1815 c         1992 g          965 t
ORIGIN      
        1 cgacgccgag ctgggcctgc accttggggt tggtcaggcc gatcttgcga ccgaccacgc
       61 ggcgaccgtt ggccacgccg tgggcgatgt tcaggcgctg gatggcgtag gccgccgcgc
      121 cgttgtcctc gccgatcagc ccgcgcagcg gctcgacggc ctctccgctc gcctcggcgc
      181 cgcgcagggc ggcggccagt tgttccaggg tctgttgagt aacgctcatg gcttacctcg
      241 ggtgcgcgca aaacgggtgc cgccgctgat ccgcaagcga atcgcggacc gttggctgtc
      301 tatgctccag caggtaacga cggtcggcgc acgaggcgct tgaccgtatc cgcactgggg
      361 cctttgttgt gggcgcgagt ataggaacgg ccgaaaaacc ccgacaacga aaaacgcctc
      421 acgcgtgcac ttagtgcaca tcattgtttt taaagaattt ttcagacaaa aagcccgcac
      481 cagccgttct cccgacaagg cgggtgacgg acggtacggg caggtaagcc ggccgcagcc
      541 agaggctgcg gcgaccgcaa aacggggccg gcgccagcgc gccagccgaa cggatcagtg
      601 gctcaggaag tccagcacca tgcggttgaa cttgtcggcg tgttcccact gcgcccagtg
      661 gccgcagcgg ttgaacacgt gcagctcggc attcggcagg ccggcgagca ggcgcaggcc
      721 ggcgtccatc ggcacgaagc ggtcgttgct gccccagatg accagggtct gcgccttgat
      781 ctcggcgagg cgcgggctga agtcggggaa ctgcttgggg ttggccgcca ggctcttgac
      841 gaagttctcc aggtggtcgc gacgattgag catgttgtcg agacgggcct ggaacagctc
      901 ctcggtgagg tcgctgctgt cgaagacgaa gacgttcatc atcttcttca ggttgtcgat
      961 ggtcggctcg cggtacaggc cctgcagcag cttgatgcct tcggtcggca tcggcgtgaa
     1021 caggctggcg ccgccggtgc caccgcccat cagcaccagc ttgccgatgc gctcggggtt
     1081 ggccagggcg aaggcgacgg cgctgtggcc acccatggag ttgccgagga tgtgcacgcg
     1141 gtccagctgc agcgcgtcga ccaggccctt gagcacgcgg gcgttgaggt cggagcgcga
     1201 gccggtgcag acgatcgagt cgctcttgct ccagcccggg cagtccatga ggatcacccg
     1261 gtagccggca gccaccagcg gctcgatgtt gcggttgaag ttggcccagc cgctggcgcc
     1321 gggaccggag ccgtgcagca tgaccacggt ttccgcacct gcgccgcagt cgttgtagtg
     1381 gatgttcagg tcgagctcgc cgtcctggat acgcacgaac tggctggtgg aggcttcgct
     1441 cagggtgata ctggtcatca actggttcct cgaaatcagg cttggggggc gaggcggcgg
     1501 aaggcgccgt tcaggtacag gagcggctcg ccgccctgct ggccacgggt ggccaggacc
     1561 tcgcagagga aagcgtggtg ggtgctctcg tcgaagacct tgaccacccg gcagtcgaag
     1621 ttggccacgg cgccgtcgag taccggcacg ccgctggcca ggtcgtgcca ctggccatgc
     1681 tggaagcgcg cctcgccgtg cacgccctcg accatgccgg cgaacacctt ggcttcgtgc
     1741 tcctgctccc cggacagcac gttcacgcac agcgcgccgc tgttgaggat cgccgcgctg
     1801 gcggcgacct tcttgttgac gaacaccacc aggcgcggcg gctcggcggt caccgaacag
     1861 accgcggtgg cggtcaggcc gagatgggtg tcgccgtcgc gcgcggtgat gatggtcacc
     1921 gcggcggcca ggttgcgcat accgacgcgg tggtcgtcgg cgctgaccac cggcagggcg
     1981 gccaggtcgg cgttggggat ccagtccagc agctgattga tttctttgct catgacacgt
     2041 gctctcacgc agcgggcaga tgtgggcggg ctctttggcc cgcccatcgg ttccgactcg
     2101 gaatcaggcc ttgacgccga acgggttggc cgccggcagc gggttgccga gcagctggcc
     2161 gccgagcagg tcgccgatgt tgtcctggtt ctgggtgatg tggttggcac cgaccaggat
     2221 gtcgcgcatc tgccgctgca tcgggctgct cagccagacg ccacgggtgg cggtcttctt
     2281 gaagatcatg tgcgcggcat cgaagcactc gccgccggtg aactggttgg tggcgaagtg
     2341 gcgcacgcgc acgtccagcg gcaccagctg gccctgggaa gcgtagctcc aggcttcctc
     2401 ggtgttgtgc agcacgcggg tggtggcacc gaggatcttg gcgtaggcct cgcccaggcg
     2461 gctcttgatg aacgggtcgt tgaccgcggc accgaccgcc tggttgagat tctgccgcgg
     2521 cttcatgtgg tcgacgtaga ggtcgaccat gccgcgcgcc atgccgatga tcaccgagga
     2581 tacggcggcg tagaacacga agaagaacgg caccttgtac aggttgttga cgtggccgtg
     2641 ggagtgctgg tcgtagtcgc cgatcacgtg gctgcgatag gtcggcacga agacttcctt
     2701 gatgagcagc ttcttgctgc cggtgccggc caggccgacc acatgccagt cgtcgacgat
     2761 ctcgaagtcg ctggcctgca cccagaacga gcggaactcc atctggccgg catcgttgac
     2821 cacgcggccg ccgaggaagg caccgccggc agcgtgatcg caaccgctgg aggtcagcca
     2881 ctcgccgttg agctgatagc cgccgtcgac acgggtgacg gtgccgaagg gcgcgtagga
     2941 ggaggacacc agacgggtgt tgtcctcgcc ccacagctcg tcgccgcagc gcgggtcgag
     3001 caggccgatc tcgaaggggt gcacaccgag caccatcacg ttccaggcgc tggacgggca
     3061 accgcgggcc agttccatca gcaccttgtt cagcacgttg gggtgcatct cgtagccgcc
     3121 ccagcgcttg ggctgcagga tcttgaagaa gccggcttcc tggaaggcct ggatggtctc
     3181 gcgggagacc atgcgcgcct tctcggtggc gtcggccttc tcgcgcagcc aggggatcat
     3241 agcctcggca cgggcaacga tttcggcttc gctggggatc tgcatatcgg tgtggctcat
     3301 ctttctatct ctcttcgccg cgagtggcgc tgcgggtgat gaatcagggt gaggctgggc
     3361 ccgcaaaggc tcagttctgc tcggcgctta ccacgccata gccggcaatc cattcgttga
     3421 tcgcccggta gtagtcctga cgggcggagt aggcgccggt ggcagccagc gccgcgaagg
     3481 cagcgaccca ggtgcgcact tcgtgcgccg agcggccggc ggccttggag atttcgtcga
     3541 tcttgaactc gtcgatctcg tcgaggcggc cctgcaccag caggtcgatg aaggccaggt
     3601 cccagtcggc gttgagcggg gtctgcagcg actccgcagt gccgtagatc ttgccggcag
     3661 cgatggtgcg cgcctggcgg gcggcccgcg cttccggggt cggattgcgc ccggcgatga
     3721 ggaactcgct gatcgcttcg ggggcgtcgg ccagcagcgg cacgggcggc tcgtgcgaca
     3781 gcccgccggt gccgaggatc agcacgcgct tgtcgagctt ggcgatgaag cggcccaccg
     3841 cctcgcccag cttgcgcacc cggcgatagg gggcgaacgg cggcgccacc gagttgatga
     3901 tgatcgggat caccggatag cggtcgaggc tgccggtcag ctcttccagg gtctgggtgc
     3961 agccgtggtc cacctgcagg cggtgcgaca gggcgatatc gacgtcgctg tcgagcacgt
     4021 ggcgagtcag ctcgagggcc aggtctttgg ccaccgacaa cgggccgggc tgggtcaggt
     4081 agtcgcccac cgactcggca gcggtggcga tcacgaacgg aggcatcagg tcgtagaaca
     4141 ggccgttgta gtgatccggc gcgaagatca cgatcagctc gggatcgaag gcctcgactt
     4201 cggcacgggc cttggccagc gccgcatcga cttcggcgac gacagcggcg cccgggtcat
     4261 tgaggccacg caggggggta tgcgacaggc atttaagttt tacagtcatg aaacctccat
     4321 gatcgtcgga gaccacccgc ctccgccgac caacgcgcaa cgtgtcaggt aagctgccgt
     4381 ggctggccgg tgtcgccgca ctggcgccgc cgggatccac tcgccatcag gctggctcgt
     4441 cacctggttg ttttttgttc agaacgacgg cggccatttt aggggcgtga ctcgggaagg
     4501 aaaaaaacta tctaccctta cgtgcactat gtgcaccatg ttgtttttaa aggttttttt
     4561 gcctttcccg gtgtgcttgc cggtcacgtt gcacggcctc gaccgctctg cgccggggat
     4621 cgggcagcga gccgcccctg cggatggcgt cgccaacggg cgcgccgggc gcggatgaaa
     4681 cgggcactgg ccggccctgc gcaggggctg gaacagcgcg gcgccgccat ctgggctggc
     4741 aggcgacagg catgcttgaa tacggcgaag gcggacttga tcaggtgaca gacagatgag
     4801 cggcacgcgc gaaaccgaat acaagaccgt acgcggcctg acccgcggcc tgcagatcct
     4861 caatgccctc aaccgggtgg acggcggcgc cagcccggcc cggctggccg agctgaccgg
     4921 cctgcaccgc accacggtgc gccgcctgct ggagaccctg caggacgagg gctacgtgcg
     4981 gcgcagcgag tcggacgaca gcttccgcct caacctcaag gtgcgcgagc tgagcgaggg
     5041 cttccgcgac gagcagtgga tttccgagct gggcgcaccg ttgctggccg agctgctgct
     5101 ggaggtgcac tggccgaccg acctgtgcac cctggacgtc gacgccatgg tggtgcgcga
     5161 gaccacccac cgcttcagcc gcctgtcgtt ccaccgctcg atggtcggcc gccgcctgcc
     5221 gatgctgatc accgccaccg gcctggccta cctggcgttc tgcccggagg ccgagcgcga
     5281 gcgcatcgtc gagctgctgg ccaagcgcga cgacgaggac tgtcgcctgg cgcgcaaccc
     5341 ggtggcgctc gacaacctgc tgcgtcgcac ccgccaccgc ggctacggcg agaacttcgg
     5401 cagctggaac agcgaggagc gcaccgcctc catcgccgtg ccgatctgcg gcgaacagcg
     5461 ggtctacggc tgcctcaacc tggtctacgt gctcaaggcg atgaccatcg agcaggccgc
     5521 ccagcgctac ctgccggcgc tgcagcgggt cgccgccacc atcgagcagg gcatccgcga
     5581 acgcgagcag gccgggccgg tgctggcgtg atccctcggg cggccggggc gtggtagaag
     5641 atcgctgcgc gagtcttcta ccctacggtt ccgctccggc cacc
//
LOCUS       DATCUN010000338         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_130854, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000338 DATCUN010000000
VERSION     DATCUN010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130854"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1233
                     /locus_tag="VIO83_12465"
     CDS             19..1233
                     /locus_tag="VIO83_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY8332125.1"
                     /translation="MNSPANLNPRPFAPRYPQLGTGPVPVSTAVDPAFFELERERIFR
                     GMWVNLILREEDLPRPGDYFVRYLEAQDASILVVRGDDGKLRAFHNVCTHRGNQVADE
                     GAGHCKGFQCNFHGWTFDTRGRLAFVTDEQRFHNLDKSALGLPAVHLDTWRGFIFVNL
                     AKRPAQTLELAMAEFNQDLKPFPFEQMVLAGRYRYAVNANWKVTMNAFQESYHGAFVH
                     KLSVGQCISPEDPYLAVQRIKLQENGNQSVSVHLAPHDLSPAEKLAFGFGATFTQGGG
                     AAQAFPGTAQAQVRDWGFDLNIIFPYSRINVGAGWYYVDAFWPVAHDRTVYELAYYFP
                     MPQRASEMISQEYSKIVLRDLSREDLATNEATHRSLRSGAIRQMNLSDQEIAVRHLYH
                     TVAARCGKELAK"
     gene            1230..1592
                     /locus_tag="VIO83_12470"
     CDS             1230..1592
                     /locus_tag="VIO83_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8332126.1"
                     /translation="MSAATALPKPFAHLECWLSFALPSEDARMRRRLSASMDELVGLY
                     RALVEEMPAIAAHLDQWPLAELPAEQKPLLYLALSFMESAMAVEAFHQPGPPHATTPE
                     SMVVFPGRAEAMLNPGNT"
     gene            complement(1771..>2138)
                     /locus_tag="VIO83_12475"
     CDS             complement(1771..>2138)
                     /locus_tag="VIO83_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8332127.1"
                     /translation="NNMPSRKKALYYALTGETFTGKQAHDLGYATKSVPLAQLDAETD
                     RVVKMLTRLGRVVLGKTKEVYEKVLDMDFEAAIDYEMAKMWELSRETNDDWVRTALVS
                     FKKREFKPGLQSYELKRDV"
BASE COUNT          359 a          722 c          685 g          372 t
ORIGIN      
        1 cctagaggag atgtgcccat gaacagtccc gcgaacctca acccgcggcc gttcgcgccg
       61 cgctatccgc agctgggcac cgggccggtg ccggtcagta ccgcggtcga cccggcgttc
      121 ttcgagctgg agcgcgagcg catctttcgc ggcatgtggg tcaacctgat cctgcgcgag
      181 gaggatctgc cccggcccgg cgactatttc gtgcgctacc tggaagccca ggacgcctcc
      241 atcctggtgg tgcgcggcga cgacggcaaa ctgcgggcct tccacaacgt ctgcacgcac
      301 cgcggcaacc aggtggcgga tgagggcgcc ggccattgca agggttttca gtgcaacttt
      361 cacggctgga ccttcgacac ccgcggccgg ctggccttcg tcaccgacga gcagcgtttc
      421 cacaacctgg acaaatcggc cctgggcctg ccggcggtgc acctggacac ctggcgcgga
      481 ttcattttcg tgaacctggc caagcgaccg gcgcagacgc tggagctggc catggccgag
      541 ttcaatcagg atctgaaacc gtttccgttc gagcagatgg tgctggccgg ccgttaccgc
      601 tacgccgtca atgccaactg gaaggtgacc atgaacgcct tccaggagtc ctaccacggt
      661 gccttcgtgc acaagctgtc ggtcggccag tgcatcagcc cggaggaccc gtacctagcc
      721 gtgcagcgca tcaagttgca ggaaaacggc aaccagtccg tgtcggtgca tctggcgccg
      781 cacgacctca gcccggccga gaagctggcc ttcggtttcg gcgccacctt cacgcagggt
      841 ggcggtgccg cccaggcttt ccccggcacc gcgcaggcac aagtgcgcga ctgggggttc
      901 gacctgaaca tcatctttcc gtactcgcgc atcaacgtcg gcgccggctg gtattacgtg
      961 gacgccttct ggccggtggc gcacgacagg acggtgtacg agctggccta ttacttcccg
     1021 atgccgcagc gggcctcgga aatgatcagt caggaataca gcaagatcgt gctgcgggac
     1081 ctgtcgcgcg aggacctggc caccaacgag gccacgcacc gctcgctgcg ctccggcgcc
     1141 atccgccaga tgaacctgtc ggaccaggaa atcgccgtgc gccacctgta ccacacggtc
     1201 gccgcccgct gcggcaagga gctggcgaaa tgagcgccgc gaccgcgttg ccaaagccgt
     1261 tcgcgcatct ggagtgctgg ctgtcgtttg cgctgcccag cgaagacgca cgcatgcgcc
     1321 gccgcctgtc ggcctccatg gatgagctcg tcggcctcta ccgggcgctg gtggaggaaa
     1381 tgccggccat cgccgcgcat ctggatcaat ggcccctggc cgaactgccg gccgagcaaa
     1441 agccgctgct gtacctggcg ctgtcgttca tggaatcggc catggccgtg gaggccttcc
     1501 accagcccgg gccgccgcat gccacgacgc cagagagcat ggtggtgttt ccggggcggg
     1561 ccgaggcgat gttgaatccg ggaaacacct gacgtatcca gcgtttcccg cagcgcctgc
     1621 cgcaacaagg catcgaaaaa ctgtaggagc ccggctatgc cgggcgatat cgcgcagcgc
     1681 agctgcgctc cacaaggtat tcacaggtgc cggccgccga gacccgcggc aggccgggcg
     1741 tccgcacctc ccgccgctgg ccagagtcgc tcagacgtcc cgcttgagct cgtaggattg
     1801 caggccgggc ttgaactcgc gcttcttgaa cgacaccagc gccgtgcgca cccagtcgtc
     1861 gttggtctcg cgggacagtt cccacatctt ggccatttcg tagtcgatgg cggcttcgaa
     1921 atccatgtcc agcaccttct cgtagacctc cttggtcttg ccgagcacca cgcgacccag
     1981 gcgcgtcagc atcttgacca cgcggtcggt ctcggcgtcc agctgcgcca gcggcacgga
     2041 tttggtggcg tagcccaggt cgtgcgcctg cttgccggtg aaggtctcgc cggtcagggc
     2101 gtagtagagc gccttcttgc gcgagggcat gttgttgg
//
LOCUS       DATCUN010000339         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_37180, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000339 DATCUN010000000
VERSION     DATCUN010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37180"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1536)
                     /locus_tag="VIO83_12480"
     CDS             complement(166..1536)
                     /locus_tag="VIO83_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003118339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY8332128.1"
                     /translation="MEQATPTLAELHQMIRMSDTINMEIFYWWCIALMIVIHAGFLSY
                     EIGASRLKNALTAGVKNILAFAFIVPTFFFFGWWIYNGFAGGIVPDLAGAAASLPWSS
                     SMGPNVQDNATGIFWAAFVLFAATTASIMSGAIIERTRMSAFIVLAIILGSVVWILGA
                     AWSWHPKGWLTTEWGFHDVGCAGAVHLIAGFFTLGVVMNVGARIGRFNADGSANPIVG
                     HSMPMSVIGLMLVIVGFFGFLGGCIIYNVGGQWTNIYGQPTTLSAFAFNALMGFAGGL
                     IGAYLTTREPFWMMSGGLVGIISVAPGLDVYYPGLSYLIGMGVAAAAPLVQNLLVKRG
                     LDDAVGAFAVHGFGGFMGLVISGLFIAGYPNVAEGAPAVNLLGQLGGAVVLAILGFFP
                     GYLISLAMKKAGWLRVPAHAEERGLDLVEVPAQAYPEWAITGAGSTVVASAAVATPAI
                     GQAKPA"
BASE COUNT          293 a          485 c          541 g          237 t
ORIGIN      
        1 cggtcagggc gacccccagc gccaggatca ccgccatcac ggccggcttg tcggcgaagg
       61 tatacacggt gctggcacct tcccagctgc tgatcggact gctcatggca ttgctccttt
      121 ggccgatgcg cggggcgggt gccgccgcgc ttacgggagt ggtgttcagg ccggcttggc
      181 ctggccgatg gcgggtgtgg cgaccgctgc gctggcgacc acggtgctac cggcgccggt
      241 gatcgcccat tccgggtagg cctgggccgg cacctcaacc agatccaggc cgcgctcctc
      301 ggcgtgcgca gggacgcgca gccagccggc cttcttcatg gccagggaaa tcaggtagcc
      361 ggggaagaag ccgaggattg ccagcaccac ggccccgccg agttggccga gcaagttcac
      421 cgccggtgcg ccttcggcga cgttcgggta accggcgatg aacaggccgc tgatcaccag
      481 gcccatgaag ccaccgaagc cgtgcacggc gaaggcaccg actgcatcgt ccaggccacg
      541 tttcaccagc aagttctgta ccagcggcgc agcggcagcg acgcccatgc cgatcaggta
      601 ggacaggccc gggtagtaga cgtcgaggcc cggggccacc gagatgatgc caaccaagcc
      661 accggacatc atccagaagg gttcgcgggt ggtcaggtag gcaccgatga ggccgccggc
      721 gaaacccatc agggcgttga aggcgaaggc ggagagggtg gtgggctggc cgtagatgtt
      781 ggtccactgg ccgccgacgt tgtagatgat gcagccgccg aggaagccga agaagccgac
      841 gatcaccagc atcagaccga tcacgctcat cggcatgctg tggccgacga tggggttggc
      901 gctgccgtcg gcgttgaagc ggccgatgcg cgcgccgacg ttcatcacca caccgagggt
      961 gaagaagccg gcgatcaggt gcaccgcgcc ggcgcagccg acgtcatgga agccccattc
     1021 ggtggtcagc cagcctttcg ggtgccagct ccaggccgcg ccgaggatcc acaccaccga
     1081 gccgaggatg atcgccagca cgatgaaggc gctcattcgt gtgcgctcga tgatcgcccc
     1141 ggacatgatc gacgcagtgg tggcggcgaa cagcacgaac gcggcccaga agatgccggt
     1201 ggcgttgtcc tgcacgttcg ggcccatgct cgagctccat ggcaggctgg ccgcggcgcc
     1261 ggccagatcc gggacgatgc cgccagcgaa gccgttgtag atccaccagc cgaagaagaa
     1321 gaaggtcggc acgatgaagg cgaaggcgag gatgttcttc accccggcgg tcagcgcgtt
     1381 cttcagtcgc gaggcgccga tctcgtagga gaggaagccg gcgtggatga cgatcatcag
     1441 cgcgatacac caccagtaga agatttccat gttgatggtg tcggacatgc ggatcatctg
     1501 gtgcagctct gcgagcgtgg gcgttgcctg ttccataggt gaaacctcga tgcgat
//
LOCUS       DATCUN010000340         5731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_73710, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000340 DATCUN010000000
VERSION     DATCUN010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73710"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3186)
                     /gene="hrpA"
                     /locus_tag="VIO83_12485"
     CDS             complement(<1..3186)
                     /gene="hrpA"
                     /locus_tag="VIO83_12485"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEY8332129.1"
                     /translation="MSAVPAFDQLLKNLDQALNADRHRLRRQLHELRKQSGTPSWDEA
                     RLAQWLERFQASVAKVEARRQSVPRIRYDDALPIAARREEIKDAISKHQVVVIAGETG
                     SGKTTQLPKICLEVGRGVHGLIGHTQPRRLAARSVATRVAEEIGTPLGELVGYQVRFE
                     DHSGEQSLIKLMTDGILLAETQHDRYLEKYDTLIVDEAHERSLNIDFLLGYLKNLLPR
                     RPDLKVIITSATIDLERFSKHFNDAPIIEVSGRTYPVDTWYRPLAAEQDENGESLFDD
                     LSVEQGILRSLDEIEAHERSVGQRPGDVLVFLPGEREIREAAEVLRKANLRHTEVMPL
                     YARLTPAEQQKIFKPAGGRKIVLATNVAETSLTVPGIRYVIDSGTARISRYSYRSKVQ
                     RLPIEAVSQASANQRKGRCGRVEPGICIRLYAEEDFLARPEFTDPEILRTNLAAVILQ
                     MLHLRLGDIQAFPFIEPPDGKAISDGFNLLQELSAVNRENQLTLLGRQLARLPIDPRL
                     GRMLLEGAQQGSLAEVLVVVSALSVQDPRERPMERQQAADQAHAQWKNPDSDFAALIN
                     LWHGFEAMRQELGANALRNWCRKNFLNYLRLREWRDAHRQLLLTCRDLQLPLNKLEAP
                     EASESRVDNGAALSTASTSQKGRGELDQRARQAVAAQDERQQNAQTLKNYAAVHKAIL
                     AGLLSQVGQKTEEGDYIGARQRRFWLHPSSVIGRKRPQWVMAAELVETTKLFARMVAK
                     IEPDWIEPLAPHLVKKNHLEPHWEKKRGQVVAYEQITLYGLIIVGRRPVHYGPIDPPA
                     ARELFIREGLVRGEINSRAKCLSANQQLLELFDELEAKARRRDILADEETLYAFYDAR
                     LPADIYQTASFETWYKRESAKNPQLLIMREEDVLAREASEITAQQYPDSLSIGRLQLP
                     LTYHFEPNHPRDGVTVRVPAPLLPQLPAERLDWLVPGLLETKCVALVRNLPKALRKNF
                     VPVPDFVGAAQAKLAFGEGSLPEALGRELTRMTGARVPEEAWADAAAQLESHLKMNIE
                     VVDAHGKFLGEGRDLAELTARFAEASQAALAL"
     gene            complement(3302..4990)
                     /gene="fadD1"
                     /locus_tag="VIO83_12490"
     CDS             complement(3302..4990)
                     /gene="fadD1"
                     /locus_tag="VIO83_12490"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016983687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD1"
                     /protein_id="HEY8332130.1"
                     /translation="MNEHFWTDKYPAGIAAEIDPDRYPNIQTVLRESCQRFADQPAFS
                     NLGHTLSYGELYELSGAFAAWLQEHTDLAPGERIAIQLPNLLQYPVAVFGALRAGLVV
                     VNTNPLYTAREMEHQFNDSGAKALVCLANMAHLAEQVLPHTGIRHVVVTEVGDLLAPL
                     KRLLVNSVVRYVKKLVPAYSLPQAVKFTEVLARGRGRPLREAQPKAAEVAVLQYTGGT
                     TGVAKGAMLTHRNLIANMLQCQALMADNLGEGCEVLITPLPLYHIYAFTFHCMAMMLM
                     GNHNVLITNPRDLSAMVGAMARWKFSGFVGLNTLFVALCNHEGFRRLDFSALKLTISG
                     GMALQQAAAERWEDITGCAICEGYGLTETSPVVSVNPRQHNQPGTIGIPMPSTLCKVI
                     DAAGNDLGLGMPGELCVKGPQVMKGYWQRPEATAEVLDADGWLKTGDIAVIQPDGYLR
                     IVDRKKDMILVSGFNVYPNELEEVLASLPGVQLGAAIGVPDEKTGEAVKLFVVRKPGA
                     ELSAEQVLAHMRANLTGYKVPRQIEFRDSLPVSNVGKILRRELRDQELKARATA"
     gene            5438..>5731
                     /locus_tag="VIO83_12495"
     CDS             5438..>5731
                     /locus_tag="VIO83_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY8332131.1"
                     /translation="MSLVENIPYDELQVGQKASYQRTVAERDIQLFAEVSGDRNPVHL
                     DAEYAATTQFKERIAHGMLTGALISAAIACTLPGPGTIYLGQNLRFTRPVKLGD"
BASE COUNT          814 a         1935 c         1992 g          990 t
ORIGIN      
        1 cagcgccagc gcggcctggc tggcctcggc gaagcgcgcg gtcagctcgg cgaggtcccg
       61 gccttcgccg aggaatttgc cgtgcgcatc gaccacctcg atattcatct tcaggtggct
      121 ttccagctgg gcggcggcgt cggcccaggc ttcctccggc acgcgggcac cggtcatgcg
      181 cgtaagctcg cggcccagcg cctcgggcag cgagccctcg ccaaaggcca gcttggcctg
      241 cgcggcgccg acgaagtccg gcaccggcac gaagttcttg cgcagcgcct tgggcaggtt
      301 gcgcaccagc gccacgcact tggtttccag caggcccggc accagccagt cgaggcgctc
      361 ggccggcagc tgcggcagca gcggcgccgg cacgcgcacg gtgacgccgt cgcgcgggtg
      421 gttgggctcg aagtggtagg tcagcggcag ctgcaggcgg ccgatgctca ggctgtccgg
      481 gtactgctgc gcagtgatct cgctggcctc gcgggccagc acgtcttcct cgcgcatgat
      541 caacagctgc ggattcttcg cgctctcgcg cttgtaccag gtctcgaagc tggcggtctg
      601 gtagatgtcc gccggcaggc gtgcgtcgta gaaggcgtac agggtttcct cgtcggcgag
      661 gatgtcacgg cggcgcgcct tggcttccag ctcgtcgaat aactccagca gctgctggtt
      721 ggcgctcagg cacttggcgc ggctgttgat ctcgccgcgc accaggccct cgcggatgaa
      781 cagctcgcgc gcggccggcg gatcgatagg gccgtagtgc accgggcggc ggccgacgat
      841 gatcagcccg tagagggtga tctgctcgta ggccaccacc tggccgcgct tcttctccca
      901 gtgcggctcg aggtggttct tcttgaccag gtgcggcgcc agcggctcga tccagtccgg
      961 ctcgatcttg gccaccatgc gcgcgaacag cttggtggtt tccaccagct cggcggccat
     1021 cacccactgc gggcgcttgc ggccgatcac gctggaaggg tgcagccaga agcgccgctg
     1081 gcgggcgccg atgtagtcgc cctcctcggt cttctggccg acctggctga gcaggccggc
     1141 aaggatcgcc ttgtgcaccg cggcgtagtt cttcagggtc tgcgcgttct gctggcgctc
     1201 gtcctgggcg gcgacggcct ggcgggcgcg ctggtcgagt tcgccgcggc ctttctgcga
     1261 cgtgctggcg gtggacaagg ctgcgccgtt gtccacccta ctctcggacg cttccggcgc
     1321 ctccagcttg ttgagcggca gctggaggtc gcggcaggtc agcagcagct ggcggtgggc
     1381 gtcgcgccat tcgcgcaggc gcagatagtt gaggaagttc ttgcggcacc agttgcgcag
     1441 ggcgttggcg cccagttcct ggcgcatggc ctcgaagccg tgccacaggt tgatcagcgc
     1501 ggcgaagtcg gaatccgggt tcttccactg cgcatgggcc tggtcggcgg cctgctggcg
     1561 ctccatcggc cgctcgcgcg ggtcctgcac cgacagggcg ctgaccacca ccagcacctc
     1621 ggccaggctg ccctgctggg cgccctccag cagcatgcgg cccagacgcg ggtcgatcgg
     1681 caggcgcgcc agctggcggc ccagcagggt cagctggttc tcgcggttga ccgccgagag
     1741 ctcctgcagc aggttgaagc cgtcgctgat cgccttgccg tccggcggct cgatgaacgg
     1801 gaacgcctgt atgtcgccca ggcgcaggtg cagcatctgc aggatcaccg cggccaggtt
     1861 ggtgcgcagg atctccggat cggtgaattc gggacgggcg aggaaatcct cctcggcgta
     1921 caggcggatg cagatgcccg gctcgacccg gccgcagcga cccttgcgct ggttggcgct
     1981 ggcctgggac accgcctcga tgggcaggcg ctggaccttg gagcggtagc tgtagcggct
     2041 gatgcgcgcg gtgccgctgt cgatcacgta gcggatgccc ggcacggtca gcgaggtctc
     2101 ggcgacgttg gtggccagca cgatcttgcg cccgccggcc ggcttgaaga tcttctgctg
     2161 ctcggccggg gtgaggcgcg cgtacagcgg catcacctcg gtgtggcgca ggttggcctt
     2221 gcgcagcacc tcggcggcct cgcggatctc gcgctcgccg gggaggaaca ccagcacgtc
     2281 gccggggcgc tggcccaccg agcgctcgtg cgcctcgatc tcgtcgagag agcgcaggat
     2341 gccctgctcc accgagaggt cgtcgaacag cgactcgccg ttctcgtcct gctcggcggc
     2401 cagcggccgg taccaggtgt ctaccgggta ggtgcgcccg gacacctcga tgatcggcgc
     2461 gtcgttgaag tgcttggaga agcgctccaa gtcgatggtc gccgaggtga tgatcacctt
     2521 gaggtcgggg cggcgcggca gcaggttctt caggtagccg agcaggaagt cgatgttcag
     2581 gctgcgctcg tgggcctcgt cgacgatcag ggtgtcgtac ttttccaggt agcggtcgtg
     2641 ctgggtctcg gcgagcagga tgccgtcggt catcagcttg atcagcgact gctcgccgct
     2701 gtggtcctcg aagcgcacct ggtagccgac cagctcgccc agcggcgtgc cgatttcctc
     2761 ggccacgcgg gtcgccacgc tgcgcgccgc caggcggcgc ggctgggtgt ggccgatcag
     2821 cccgtgcacg ccgcggccga cctccaggca gatcttcggc agctgggtgg tcttgcccga
     2881 accggtctcg ccggcgatca ccaccacctg gtgcttgctg atcgcgtcct tgatctcctc
     2941 gcggcgcgcg gcgatcggca gcgcatcgtc gtagcggatg cgcggcacgc tctgccggcg
     3001 cgcctcgacc ttggccacgg aggcctggaa gcgctccagc cactgcgcca gcctggcctc
     3061 gtcccacgat ggcgtgcccg actgcttgcg cagctcgtgg agctggcggc gcaggcggtg
     3121 gcggtcggcg ttgagcgcct ggtcgaggtt cttcagcaac tggtcgaagg cgggtacggc
     3181 actcatcggg cgggcgtcgg cttggtattt tcggggcggg gattgtcgca gatttgcgcg
     3241 gggaatggcg gcggtgcgga tcgtgccccc gcgcgggcac gatccgcacc ggacgacagc
     3301 ctcaggccgt agcgcgcgcc ttcagctcct ggtcgcgcag ctcgcggcgc aggatcttgc
     3361 cgacgttgct gaccggcagg ctgtcgcgga actcgatctg ccgcggcacc ttgtagccgg
     3421 tgaggttggc gcgcatgtgc gccagcacct gttcggcgct cagctcggcg ccgggcttgc
     3481 gcaccacgaa cagcttgacc gcttcgccgg tcttctcgtc cggcacgccg atggcggcgc
     3541 ccagctgcac ccccggcagg ctggccagca cttcctccag ctcgttgggg tacacgttga
     3601 agccggagac caggatcatg tccttcttgc ggtcgacgat gcgcaagtag ccatccggct
     3661 ggatcaccgc gatgtcgccg gtcttcagcc agccgtcggc gtccagcacc tcggcggtgg
     3721 cctcggggcg ctgccagtag cccttcatca cctgcgggcc cttcacgcac agctcgccgg
     3781 gcatgccgag tccgaggtcg ttgccggccg cgtcgatcac cttgcacagg gtcgacggca
     3841 tgggaatgcc gatggtgccg ggctggttgt gctggcgcgg gttgaccgac accaccgggc
     3901 tggtctcggt caggccgtag ccttcgcaga tggcacagcc ggtgatgtcc tcccagcgct
     3961 cggcggcggc ctgctgcagg gccatgccgc cggagatggt cagcttcagt gcagagaagt
     4021 cgaggcggcg gaaaccctcg tgattgcaca gggcgacgaa cagggtgttg aggccgacaa
     4081 agccgctgaa cttccaccgg gccattgcgc cgaccatcgc cgacaaatcg cgcgggttgg
     4141 tgatcagcac gttgtggttg cccatcagca tcatcgccat gcagtgaaag gtgaaggcgt
     4201 agatgtggta cagcggcagc ggcgtgatca gcacctcgca gccttcaccg aggttgtcgg
     4261 ccatcagcgc ctggcactgc agcatgttgg cgatcaggtt gcggtgggtg agcatggcgc
     4321 ccttggccac gccggtggtg ccgccggtgt actgcagcac cgccacctcg gcagccttgg
     4381 gttgcgcctc gcgcagcggc cggccgcgtc cgcgcgccag cacctcggtg aacttcaccg
     4441 cctgcggcag gctgtaggcc ggcaccagct tcttcacgta gcgcaccacg ctgttgacca
     4501 acaggcgctt gagcggcgcc aacaggtcgc cgacctcggt gacgaccaca tggcggatgc
     4561 cggtatgcgg cagcacctgc tcggccagat gcgccatgtt ggccaggcag accagcgcct
     4621 tggcgccgga gtcgttgaac tggtgctcca tctcccgcgc ggtgtacagc gggttggtgt
     4681 tgaccaccac cagcccggcg cgcagggcgc cgaacaccgc gacgggatac tgcagcaggt
     4741 tgggcagttg gatggcgatg cgctcgcccg gcgccagatc ggtgtgctcc tgcagccagg
     4801 cggcgaaggc gccggagagc tcgtacagct cgccgtagct gagggtatgg ccgaggttgc
     4861 tgaacgccgg ctggtcggca aagcgctggc aggactcgcg cagcaccgtc tggatgttcg
     4921 ggtagcggtc ggggtcgatc tcggcagcga tccccgcggg gtatttgtcg gtccagaagt
     4981 gttcgttcat cgaggctctc tcccgagcga cggcagccgc gcctgtcagc cggtcccgga
     5041 caggcgcaac gccccgcctc tgtgcgccgg ccatggcgct cttgttatgt ccggtccgca
     5101 ccaccactgc cgccagcggc accgcgagga ccatcgtcca gcgagaataa cagcttcccc
     5161 cacgggcgcg tctgcgcggg agcgcaagcc gttcaactca ccctagccca atcagctgcg
     5221 ccacagcagc gtcacggcgg ccatcctgcc cgacatcgac tacccgccgc tctggctgac
     5281 gcagttcagc cgcttcatgc gtgctccctt gtgtagtagc gggcctgggc tttcctgtaa
     5341 cgtagcctgc catccgcttg cgccggacac ctccgtcgtg cgccggcgtc agccgacgcc
     5401 aaccatccgt atagccatgc aaaccctagg agagcgcatg agtctggtag aaaacatccc
     5461 ctacgatgaa ctgcaggtcg gtcagaaagc ctcctaccag cgcactgtgg cggaacgcga
     5521 cattcagctg ttcgccgagg tttccggcga ccgcaacccg gtgcacctgg atgccgaata
     5581 cgccgccacc acccagttca aggagcgcat cgcccacggc atgctcaccg gtgcgctgat
     5641 cagcgcggcc atcgcctgca ccctgccggg gccgggcacc atctacctcg gccagaacct
     5701 gcgcttcacc cgtccggtga agctcggcga c
//
LOCUS       DATCUN010000341        18894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U7359 k141_149929, whole
            genome shotgun sequence.
ACCESSION   DATCUN010000341 DATCUN010000000
VERSION     DATCUN010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955172
            Sequence Read Archive: SRR2901746
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 18894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:48:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,515
            CDSs (total)                      :: 2,478
            Genes (coding)                    :: 2,440
            CDSs (with protein)               :: 2,440
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 29 of 38
            Pseudo Genes (internal stop)      :: 8 of 38
            Pseudo Genes (multiple problems)  :: 11 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18894
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149929"
                     /isolate="SMAG_U7359"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="Canada"
                     /lat_lon="48.4244 N 123.3881 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VIO83_12500"
     CDS             complement(<1..330)
                     /locus_tag="VIO83_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020289308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEY8332132.1"
                     /translation="MSYCKQNNCEGLILRILWMVLFVLVWQVAELVLLAVVVVQLVYR
                     LFKGRPNVDVMNFGDSLSQYLAQIGRFGSFHSDEKPWPFTDWPTPREPQGEGAEAVRA
                     AAEAAAPA"
     gene            complement(357..1379)
                     /locus_tag="VIO83_12505"
     CDS             complement(357..1379)
                     /locus_tag="VIO83_12505"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY8332133.1"
                     /translation="MTEQQPIAVLGGGSFGTALANLLAENGQRVRLWMRDAAQAEAIR
                     VKRENPRYLKGIKVHAGVEPVTDLAATLQACDLVFVVLPSSALRAALQPVADLLAGKL
                     LVSTTKGIEANTFKLMSQILEEIAPQARIGVISGPNLAREIAEHALTATVVASEDEEL
                     CRRVQQVLHGRTFRVYASRDRFGVELGGALKNVYAIMSGMAVALGMGENTKSMLITRA
                     LAEMTRFAVQMGANPMTFLGLAGVGDLIVTCSSPKSRNYQVGYALGQGLSLEDAVTRL
                     GEVAEGVNTIRVLKARADQLGVYMPLVAGLHAILFEGRTLEQVIGLLMSGEPKTDVDF
                     ISASGF"
     gene            1533..3674
                     /locus_tag="VIO83_12510"
     CDS             1533..3674
                     /locus_tag="VIO83_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8332134.1"
                     /translation="MVGCIRIALALLAGALLTGKALAADLFASTPELPAVLTATRLQQ
                     APAAVPGSVSVIDKELIRASGARELPELLRLVPGMLVVPNGNTTTVNYHGSSANQARR
                     LQVLVDGRSVYRSGFAQVDWNDIPLAIEDIERIEVFRGPNTVSYGANALMAVVNILTR
                     APRDSHGSRLKVTQGQRGINDWYASHGFGWSGGDLRLSLSGLEDDGFDHKADGSDFRD
                     SQRLSRLNLRVNQELDASQSLDWQLALKEGSNQIDNHYQPVFPIAAQPWEQDGDADVR
                     ARDYAGSLRWNLDLSAEHSLYVQGNVQHWERLRQWRACEGQLAFSPELRDLWNRDPDY
                     VLAVGEAVTEGLPLPGTDAQKAAIAAILAQSPPALAPATGKPVFAHSCGLINEDVRES
                     RFDLEIQDTLSLSDNLRLVSGLSYRHDRADSQVYLNGARSKDTWSLFSQFEWHIDPHW
                     LLQGGAMFEDDTSVGSSLSPRIALNYLLTPAHGLRAVYSEAVRSPDMFENHVDWQYRV
                     RDLRPIALGQREARYFASARGPGDLEQERMRSRELGYHGSFAELGLNLDVKLFYDEIS
                     DLISEPLKVYDFTPSNDNHLRLKGVESELDWQVTAHDRLRLGHAYIEFDASHRIDQRL
                     TPRHSGSLAWLHDWGRGWSSSLFYYGADSLNEYRFERTDVRVAKRLQFGSHRLELAGL
                     LQQRLDDEPLGWENNNYDERQVFWFSAEFEL"
     gene            3693..4598
                     /locus_tag="VIO83_12515"
     CDS             3693..4598
                     /locus_tag="VIO83_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017132975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8332135.1"
                     /translation="MSILYHFAALACLLALLVWPPSSMASEILLAASNDSPALRRFVA
                     DLAARRPGDRVRLVPASELTTPSALPADSRLVLLGADALDWRLSDPGGPPTLVMQISR
                     VQAHKRLKDSQPAHLTLLWSDPSPERQLHLIRQLLPQARRIGLLYGDDSRFLVNEVRQ
                     HAAAQGLAINAWFWPDTRDSRPLSRMLDESDVLLGVDDPALFNPRTIKSVLLASYGRK
                     QVLIGPSAAFVPAGSLSSSYSDQRDWLNSLDELLSQPPSQWPRAAYPRYFKVLSNPQV
                     ARSLGIELSDDVDQAGRLQHWESNK"
     gene            4598..6523
                     /locus_tag="VIO83_12520"
     CDS             4598..6523
                     /locus_tag="VIO83_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8332136.1"
                     /translation="MMRWGQSDIRTRTLAISLGPALLLTLLLTGYFTYTRLQDLRQEL
                     TQTGQQIANQLAPATEYGVISGNLPILDSLLRASLDTPHMRFIEVRDLEGEVLAYVEQ
                     AHEMREARIEVFEAPILRQTITLDDSFLLTPPQPQADTGQDVLGLVLVGMTDEAFSER
                     QQQILLRAGGLAVLALALTLLMAYRLARSLAEPIRTMGRAVQAIQGGEYHTELPTDDR
                     GELGELARHINRMASALEQAQSEQQRSITELIAAREEAERANRAKSDFLAMMSHELRT
                     PMNGVLGMLQLLETTEQSSEQVEYTTLATESTEHLLKVINDILDFSRIERGSLALENI
                     PFRLLELIVSSVQVFRPSAQQRGLQLLMDNQPGLESIEVRGDPTRLRQILVNLLGNAL
                     KFTEEGSITVTTRWQVLDNQTLWLTCAVHDTGIGIPASRLQHMFDAFEQADTSTSRRY
                     GGTGLGLSIARTLAEHMGGNLRAESVVGQGSTFTLEIPLPWRQVEVEEQAILAGTQDQ
                     GAGETVLLVEDNPVNQTVIEAMLRSLGFQVQLVSDGLQALQAVREQAYAAILMDCRLP
                     GLDGYAATRQIRAAEGPNRDAPIIALTANALQGDRETCLAAGMNDYLAKPFKRSDLSL
                     ALQRWIGSRPSTGGESV"
     gene            6710..7225
                     /gene="fabA"
                     /locus_tag="VIO83_12525"
     CDS             6710..7225
                     /gene="fabA"
                     /locus_tag="VIO83_12525"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HEY8332137.1"
                     /translation="MTKQNAFTQEELLSCSRGELFGPGNAQLPAPNMLMMDRIVHISE
                     HGGKYGKGEIVAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWQGNPGR
                     GRALGSGEVKFFGQVLPTAKKVTYNIHIKRTITRSLILAIADGTVSVDGREIYSAEGL
                     RVGLFTSTDSF"
     gene            7237..8457
                     /gene="fabB"
                     /locus_tag="VIO83_12530"
     CDS             7237..8457
                     /gene="fabB"
                     /locus_tag="VIO83_12530"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HEY8332138.1"
                     /translation="MRRVVITGMGIVSCLGNDKETVSANLRASRPGIRFNQSYADMGL
                     RSQVSGSVDLDLEALIDRKVYRFMGDAAAFAYLSMEQAIKDAGLTEEEISNPRVGLIA
                     GSGGASTLNQMEALDILREKGVKRVGPYRVPRTMGSTVSACLATPFKIKGVNYTISSA
                     CATSAHCIGNAMEQIQMGKQDMVFAGGGEEEHWSQSFLFDAMGALSTQYNATPEKASR
                     AYDAKRDGFVIAGGGGMVVVEELEHALKRGAKIYAEIVGYGATSDGYDMVAPSGEGAI
                     RCMQQALATVDTPIDYLNTHGTSTPVGDVAEARAVRTVFGDKAPAISSTKSLSGHSLG
                     AAGVQEAIYSLLMMEGNFIAGSANIDELDPEVADLPIVTQTRENVTLNTVMSNSFGFG
                     GTNATLVLKRWTGK"
     gene            8649..8840
                     /locus_tag="VIO83_12535"
     CDS             8649..8840
                     /locus_tag="VIO83_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017337873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEY8332139.1"
                     /translation="MQCNVGKTDKILRIVAGLVLLALGATGVIGWWGLIGLVPLATGL
                     LSWCPAYGLLGIKTCGKDQ"
     gene            8842..9276
                     /locus_tag="VIO83_12540"
     CDS             8842..9276
                     /locus_tag="VIO83_12540"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY8332140.1"
                     /translation="MTLKTIRASGNLDHAFAISVEAGNHVIRMDQPKNAAGQDLGPTP
                     LELILAAVAGCFGTIGRFLAHQRKFELRGMQFEIEGDYDPDGLLGRRDDVRPGFQALR
                     VKVAIDADLTREEKQAVLAEIERRCPLADNLVNGTRLESTLA"
     gene            complement(9342..9553)
                     /locus_tag="VIO83_12545"
                     /pseudo
     CDS             complement(9342..9553)
                     /locus_tag="VIO83_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009396448.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3859 domain-containing protein"
     gene            9656..11473
                     /gene="ftsH"
                     /locus_tag="VIO83_12550"
     CDS             9656..11473
                     /gene="ftsH"
                     /locus_tag="VIO83_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY8332141.1"
                     /translation="MDKKFQWNAWYFLLAFSLLLMFQGWWSERQAVEPIAYSEFLKLL
                     KDGKLSELSINNQQISGRLLEPINNRSSFVTNRVDPALAEELAASGAKFTAVRENTLV
                     ANLLSWLLPLVLIFAFWSFLFRRLTDKQGLGGLVSVGKSKAKVFVERDTGVTFEDVAG
                     IDEAKAELEEIVSFLKDQPKYSRLGARIPKGILLVGPPGTGKTLVAKAVAGEAGVPFF
                     SISGSEFVEMFVGVGAARVRDLFEQARKAAPCIIFIDELDALGKMRGVGAFGGNDEKE
                     QTLNQLLAELDGFDPREGVVLLAATNRPEILDPALLRAGRFDRQIVVDRPDRKGRVAI
                     LKVHVKRIQIAADVDLDQVAGITPGFTGADLANLVNEAAILATRRSAEQVAMDDFTRA
                     VERIVAGAERRGRLLLPHEREVVAYHEMGHALVASSLPGMDPVHKVSIIPRSIGALGY
                     TMQRPTDDRFLITLGELKNRMVVLLAGRAAEQLLFGEISTGAADDLVKVTDIARQIIT
                     RFGMSADLGQAVLERQSNSFLGDNLIAMREKDYSEQTAREIDLAVRQLIDEAYRRAGE
                     VLQQRRAELDEGARLLLERETITPEDFAPLRPERAAAAP"
     gene            11572..12597
                     /locus_tag="VIO83_12555"
     CDS             11572..12597
                     /locus_tag="VIO83_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8332142.1"
                     /translation="MSIDHRPLIYQAYLYTLAFALLAVGGISLLDVLLQQQPSPYRML
                     LLPDSALLALALGATLLAAVLRQRRACLLACASVLLLMSYTLLYNNLYGGAEQGRSLL
                     GDSPRLHSEPVLLLGLLAVVLLAGCLDGRLARRLGELAGALVMALGLLGLLARIWPAL
                     APPVPGFMLEVPTLSGLTLIFLGLAVLLLNRLGTLPGLAPFDRRTLLAGVCGVLLSTA
                     SWALLSQQSINALSEQSQLLLARLQSVSRETLSARLALIQRMAERWQASASMPSAPLW
                     QQEAGSYLRDFPNLGLLAVLDERLAPQRSQVRAPMAQLWLESFLADPARRGVTGCSRC
                     APGARRR"
     gene            12594..14615
                     /locus_tag="VIO83_12560"
     CDS             12594..14615
                     /locus_tag="VIO83_12560"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8332143.1"
                     /translation="MSASLNLLGNGPMALIAVPLHLPGQQAQLLVASLDVGDTLGSLL
                     GGNLGEFIIRVHEGGQLIYDSAGTRPAHDPTPVGSGQTRLAQTPEWTIGTYLEPGRGQ
                     AGSRLLAILVLLAGLALSFFLMFTQRLAWGAQQQALHLRERQRFDEAQSRVLDMISTL
                     QPLPQILEAICRLIEQRDPRLHCSILLVDSSGQRLTLGAAPSLPEHYNQAVDGLPIRE
                     GTGSCGTAAFRRQAVLVNDIAHDPLWQGFHELAASSDLHACWSTPLFAASGDLLGTFA
                     IYQQQAMVVDTALEELVATASHLAAIAIEHKTTRQRLQESEQRFRSLFACNPDPVFAF
                     DLRGCFERVNPVASQLLGYREDELRGAHFTQYLAPTEAQRIQPLLLKVARGETHAFEL
                     QCHNRAGELLELDVTQLPIVIDRAIVGVFCIAKDIRQRKSAERTLQSTLAELERSNRE
                     LQEFAFVASHDLQEPLRKIQTFAERLRTRPAGLDEQGRDYLQRMSSAAERMQALIHDL
                     LAYSRVATRTQPFVRLHLERVLDAVLQDLEHVVERSGAQIVRAPLPAIAGDPSQMRQL
                     LQNLLSNAIKFHKPGELPQVRIYAEHQPTGEWSLCVSDQGVGFDEKYLDRIFHPFQRL
                     HSRQEYPGTGIGLAIVNKIAERHGARLSASSQPGQGATFRVTFPAADKE"
     gene            14622..15080
                     /locus_tag="VIO83_12565"
     CDS             14622..15080
                     /locus_tag="VIO83_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8332144.1"
                     /translation="MSSPLPAPILIADDDPDDCLLAREAFSESRIPNRLEFVHDGEQL
                     LDYLHHRPPYDDARRYPLPGLIVLDLNMPRMDGRTALRALKDDPQLRQIPVIMLSTSS
                     DAEDIRRSYADGVNSFISKPASYSGLLEVVRSLSGYWLNTVELPLEPPPS"
     gene            15077..17194
                     /locus_tag="VIO83_12570"
     CDS             15077..17194
                     /locus_tag="VIO83_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8332145.1"
                     /translation="MTLPDAPLRLLLIEDDEDDFLIIRDLLGEAGNLHYVLDWADSFE
                     RGRCAIDSGVHDLYLIDNRLGTDSGLELVRHAIARNMHQPLILLTGRDDSELDARALE
                     LGASDYLVKGQFDGRLLGRSIRYALERASARERLGRSDNRAALLQRCLEASYNGVLIV
                     DAQAADQPIIYANPAFERITGYREEEVLGRNCRFLQGGETRQPAIGEIRRSLAEHREV
                     HVVLRNFRKDGSPFWNDLYIAPVPDERGVVTHYVGVQNDISERKRFESELAYNASHDV
                     LTGLPNRSLLEDRLLQGCQIARRYSRELAVMLIDLDGFKPINDTLGHAVGDKVLVEVA
                     QRMGAQIRPGDTLARLGGDEFVVLLPDLAHGEDVLLVAERLIRSISRPYRLNELELHV
                     TASIGITLSDGEIEQPMKLIQQADLAMYQAKQHGRNNYQWYTEHLNQEVSERMTLRNE
                     LQKAIESMSFELHYQPQIDAHSGAVIGSEALLRWPHAERGLISPLRFIPVAEDTGQII
                     PISQWVLDTACRHNRQLLDRGLARGAISVNISAVHFLRSNFIETVRRALDSSGLPASL
                     LELEITESVLLKNAERAIYLLHELKKIGVGLALDDFGTGYSSLSYLKDLPIDKVKIDR
                     SFVQELISDPRDAAITQGIISMAHHLNLKVVAEGVESAEQAAFLNRNQCDAFQGFLYA
                     RPMPFAELEGYLQRQAQLEMAGH"
     gene            complement(17247..>18894)
                     /locus_tag="VIO83_12575"
     CDS             complement(17247..>18894)
                     /locus_tag="VIO83_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY8332146.1"
                     /translation="TLGAHCWQSAQARLCADAQRLLPDPQLRMRLRGFDLARLAGALP
                     EDFAMVGQLGGDLRLDLPASGPSGELRLDAGSGQLRLRQRTDGHEQWLDVPYRQLSLV
                     SQLRPQRVDSQLRLAGPSLGTLELDTVIDPRPASRPLSGSFRLDGLDLALARPFVSQV
                     ERLEGRIDGRGQLSGSLLAPVVNGRVQLRDGHVSGGELPLTFEALQLTMTIAGQQALV
                     DGRWRSGEHGQGALTGSLDWAVAPQMDLQIRGSRLPAVVEPYASVEVEPDLQLGLRDH
                     QFSLGGQLAIPRGQIKVRELPAQAVTVSGDAQIIGEQTAAARMPGLKMDLRLALGEDR
                     LRFSGFGLSADLKGHLRIKDNLDSRGTLKLENGRYRAYGQRLTLRRAQLIFAGPIDQP
                     LLDIEAIRTVDEVTAGLRLTGRADAPVSEVFSEPAMSQEQALSYLVLGRAPGSSGDSN
                     LVGQAALALGLAGGTPVAGAIAQRLGVRDFQLDSEGSGTSSSVFASGYLSEKLSLRYG
                     VGVFEQGNIFALRYELGKKLYLEAASGLASSLDIFYKRDY"
BASE COUNT         3258 a         6768 c         5998 g         2870 t
ORIGIN      
        1 ggcaggggca gcggcctcgg cggcggcgcg tacggcctcg gcgccctcgc cctgcggctc
       61 gcgcggggtc ggccagtcgg tgaacggcca gggcttctcg tcgctgtgga agctgccgaa
      121 gcggccgatc tgcgccaggt actggctgag actgtcaccg aagttcatca cgtccacatt
      181 cggccgcccc ttgaacaatc ggtagaccag ttgcaccacc accaccgcca gcagcaccag
      241 ttcggcaacc tgccagacca gcacgaacag caccatccat aggatccgca ggatcaggcc
      301 ttcacaattg ttctgtttgc agtaggacat gcaactcgct cccttgcgta tgcaatctag
      361 aaaccgctgg cggaaatgaa atcgacgtcg gtcttcggct cgccggacat cagcaggccg
      421 atgacctgtt cgagggtgcg accctcgaaa agtatggcat gcaggccggc aaccagcggc
      481 atgtacacac ccagctggtc ggcgcgggcc ttgagcacgc ggatggtgtt gaccccctcg
      541 gccacctcgc ccagccgcgt caccgcatcc tccaggctca gcccctggcc cagagcgtag
      601 ccgacctgat agttgcggct cttcggtgac gaacaggtga cgatcaggtc gccgaccccc
      661 gccaggccga ggaaggtcat cgggttggcg cccatctgca cggcgaagcg ggtcatctcg
      721 gccagcgcgc gggtgatcag catgctcttg gtgttctcgc ccatgcccag cgcgacggcc
      781 atgccggaca tgatggcgta gacgttcttc agcgccccgc ccagctcgac gccgaagcgg
      841 tcgcgactgg cgtagacgcg gaaggtgcgc ccgtgcagca cctgctgcac gcgccggcag
      901 agctcctcgt cctcgctggc gaccaccgtg gcggtcagcg cgtgctcggc gatctcgcgc
      961 gccaggttcg gcccggaaat caccccgatg cgcgcctgcg gggcgatttc ctcgaggatc
     1021 tgactcatca gcttgaaggt gttcgcctcg atgcccttgg tggtgctgac cagcagcttg
     1081 cccgccagca aatcggccac gggctgcagc gccgctcgca gggccgagga cggcagcacg
     1141 acgaacacca gatcgcaggc ctgcagggtc gccgccagat cggtgaccgg ctcgacgccc
     1201 gcatgcacct tgatgccctt gagatagcgc gggttctcgc gcttcacccg aatcgcctcg
     1261 gcctgcgccg cgtcgcgcat ccacaggcgc acgcgctggc cattttccgc cagcaggttg
     1321 gccagcgcgg tgccgaagct gccgccgccc agcacggcaa tgggttgctg ctcagtcatg
     1381 atgaatccgt atccagccgc cacgcggcaa tcgctgcatt atacggggcc cccgccggcc
     1441 taccagcaca ctgtgattgc cctcgctggc aaaatgcgtc gcctcgggta ccatgcgcgc
     1501 ccgcacattt gttcagacta ggggatgccg ccatggttgg ctgcatacgc atcgcactgg
     1561 cgctgctggc cggcgcgctg ctgaccggca aggcacttgc cgcagacctc ttcgccagca
     1621 cgcccgagct gcccgcagtg ctcaccgcca cgcgcctgca gcaggcaccg gcggcagtac
     1681 cgggcagcgt cagcgtgatc gacaaggaac tgatccgcgc cagcggcgcc cgcgagctgc
     1741 cggagctgct gcggctggtg ccgggcatgc tggtggtgcc gaacggcaac accaccaccg
     1801 tcaactacca cggtagcagc gccaaccagg cccgccgcct gcaggtgctg gtggacggcc
     1861 gctcggtgta tcgctcgggc ttcgcccagg tcgactggaa cgacatcccg ctagccatcg
     1921 aggatatcga gcgcatcgaa gtgttccgcg gccccaatac ggtcagctac ggcgccaacg
     1981 cgctgatggc ggtggtcaac atcctgaccc gcgccccgcg cgacagccac ggcagccgcc
     2041 tgaaggtgac ccagggccag cgcgggatca acgactggta cgccagccac ggcttcggct
     2101 ggagcggcgg cgacctgcgc ctgtcgctgt ccggtctgga ggatgatggc ttcgaccaca
     2161 aggctgatgg cagcgatttt cgcgacagcc agcggctcag ccggctcaac ctgcgtgtca
     2221 accaggagct ggatgccagc cagtccctcg actggcagct ggcactcaag gaaggcagca
     2281 accagatcga caaccactac cagccggttt tcccgatcgc cgcccagccg tgggaacagg
     2341 acggcgacgc ggacgtgcgc gcccgcgact atgccggcag tctgcgctgg aatctcgacc
     2401 tcagcgccga acacagcctg tacgtgcagg gcaacgtgca gcactgggag cgtctgcgcc
     2461 agtggcgggc ctgcgaaggg cagctggcgt tcagtccgga gttgcgtgat ctttggaacc
     2521 gcgacccaga ctacgtgctc gcggtcggcg aagcggtcac agagggcctg ccactccctg
     2581 gaacggatgc gcaaaaagcc gcgattgcag caatcctcgc gcaatcccca ccagccctcg
     2641 ccccggcaac cggcaagccg gttttcgccc attcctgcgg cctgatcaac gaagatgtgc
     2701 gcgagagccg cttcgacctc gaaatccaag acaccctcag cctgtccgac aacctgcgcc
     2761 tggtcagtgg cctgagctac cgccacgacc gggccgattc gcaggtctac ctcaacggcg
     2821 cgcgcagcaa ggacacctgg agcctgttca gccagttcga gtggcatatc gacccgcact
     2881 ggctgctgca gggcggcgcc atgttcgagg acgacacctc ggtcggcagc tcgctgtcgc
     2941 cgcgcatcgc cctcaactac ctgctcaccc cggcccacgg tctgcgcgcg gtgtattccg
     3001 aagcggtgcg ctcgccggac atgttcgaga accatgtcga ctggcaatac cgcgtgcgcg
     3061 acctgcgtcc gatcgctctc ggccagcgcg aggcgcgcta cttcgccagc gcccgcggac
     3121 ccggcgatct cgagcaggag cgcatgcgct cgcgggaact gggctaccac ggcagcttcg
     3181 ccgaactcgg cctcaacctc gacgtcaaac tgttctacga cgagatcagc gacctgatca
     3241 gcgagccgct caaggtgtac gatttcaccc cgagcaacga caatcacctg cgcctcaagg
     3301 gcgtcgagag cgaactggac tggcaggtga ccgcccatga tcgcctgcgc ctcggccatg
     3361 cctatatcga attcgacgcc agccaccgga tcgaccagcg cctgacgccg cgccatagcg
     3421 gctcgctcgc ctggctgcac gactggggtc gcggttggtc gagcagcctg ttctactatg
     3481 gagctgatag tcttaacgag taccgattcg agcgcaccga cgtgcgggtg gccaaacgcc
     3541 tgcagttcgg cagccaccga ctggaactgg ccgggctgct gcaacagcgc ctggacgatg
     3601 agccgctcgg ctgggagaac aacaattacg acgagcgcca ggtgttctgg ttctccgccg
     3661 agttcgaact ctgacccatg ccgaactcaa ggatgagtat tctgtatcac ttcgccgcgc
     3721 tggcctgcct actggccctg ctcgtctggc cgccgtccag catggccagc gagatcctgc
     3781 tcgccgccag caacgacagc ccggccctgc gccggttcgt cgctgacctg gccgcgcgcc
     3841 ggccgggaga tcgggtgcgc ctggtgcccg ccagcgaact gaccacgccc tcggccctgc
     3901 ccgccgacag ccgcctggtg ctcctcggtg ccgacgcgct ggactggcgc ctgagcgatc
     3961 cgggcgggcc gcccaccctg gtcatgcaga tcagccgggt gcaggcccat aagcgcctga
     4021 aagacagcca gccagcgcat ctgaccctgt tgtggagcga tccgtcgccg gagcgccagt
     4081 tacacctgat ccgccagctg ctgccgcagg cacggcggat cggcctgctg tatggcgacg
     4141 acagccgctt cctggtcaac gaagtgcgcc agcatgcggc cgcgcaggga ctggcgatca
     4201 acgcctggtt ctggccggac acccgcgaca gccgcccact gagccggatg ctcgacgaaa
     4261 gcgacgtgct gctcggcgtg gatgatccgg ccctgttcaa cccgcggaca atcaagagcg
     4321 tgctgctcgc cagctacggc cgcaagcagg tgctgatcgg accgagcgcg gccttcgttc
     4381 ccgccggcag cctgagcagc agctacagcg accagcgtga ctggctcaac agtctcgacg
     4441 aattgttgtc gcagccgccg tcgcaatggc cccgcgccgc ctatccacgc tatttcaagg
     4501 tactcagcaa cccgcaggtc gcccgctcgc tgggcatcga gctgagcgac gacgtcgacc
     4561 aggccggacg cctgcagcac tgggagagca acaaatgatg atgcgctggg gacagagcga
     4621 tatccgcacc cgcaccctgg ccatcagcct tggcccggcg ctgctgctga ccctgctgct
     4681 gaccggctac ttcacctaca cccgcctgca ggacctgcgc caggagctga cccagaccgg
     4741 ccagcagatc gccaaccagc tggcgccggc caccgaatac ggcgtgatct ccggcaacct
     4801 gccgatcctc gacagcctgc tgcgtgccag cctggacacc ccgcacatgc gcttcatcga
     4861 ggtacgcgat ctcgaaggcg aggtgctcgc ctatgtcgaa caggctcacg agatgcgcga
     4921 ggcgcggatc gaagtgttcg aggcgccgat cctgcgccag acgatcacgc tcgacgactc
     4981 cttcctgctc acgcctcccc agccgcaggc cgataccggc caggacgtcc ttggcctggt
     5041 gctggtcggc atgaccgacg aggccttcag cgagcgccag caacagatcc tgctgcgtgc
     5101 cggcggcctg gccgtgctgg ccctggccct gaccctgctg atggcctatc gcctggcacg
     5161 cagcctggcc gagccgatcc gcaccatggg ccgcgccgtg caggcgatcc agggcggcga
     5221 gtaccacacc gagctgccaa ccgatgaccg cggcgagctc ggcgaactgg cgcgccatat
     5281 caaccgcatg gccagcgcac tggagcaggc gcagagcgaa cagcaacgca gcatcaccga
     5341 actgattgcc gcccgcgagg aagccgagcg ggccaaccgc gccaagtcgg acttcctggc
     5401 gatgatgagc cacgagctgc gcacgccaat gaacggtgtg ctgggcatgc tgcaactgct
     5461 ggaaaccacc gagcagagca gcgagcaggt cgaatacacc acgctggcca ccgaatccac
     5521 cgagcacctg ctcaaggtga tcaacgacat cctcgacttc tcgcgcatcg agcgtggctc
     5581 gctggcgctg gagaatatcc cgttccggct gctcgagctg atcgtcagct cggtgcaggt
     5641 atttcgcccc agcgcccagc agcgcggcct gcagttgctg atggacaacc agccgggcct
     5701 ggagagcatc gaggtgcgcg gcgatcccac ccgcctgcgc cagatcctgg tcaacctgct
     5761 cggcaatgcg ctgaagttca ccgaggaagg cagcatcacc gtgaccaccc gctggcaggt
     5821 gctcgacaac cagaccctct ggctgacctg cgcggtgcac gacaccggca tcggcattcc
     5881 ggccagccgt ctgcagcaca tgttcgacgc cttcgagcag gccgacacct cgacctcgcg
     5941 ccgctatggc ggcaccggcc tcggcctgtc catcgcccgc accctggccg agcacatggg
     6001 cggcaacctg cgggccgaaa gcgtcgtcgg ccagggctcg accttcaccc tggaaatccc
     6061 cctgccctgg cgccaggtgg aggtcgagga acaggcgatc ctcgccggca cccaggacca
     6121 gggcgccggc gagaccgtgc tgctggtaga ggacaatccg gtcaaccaga cggtgatcga
     6181 ggccatgctg cgcagcctcg gctttcaggt ccagctggtc agcgacggcc tgcaggccct
     6241 gcaggccgtc cgcgagcagg cctacgcggc gattctcatg gactgtcgcc tgcccggtct
     6301 ggacggctat gccgccaccc ggcagatccg cgccgcagag ggaccgaacc gtgacgcgcc
     6361 gatcattgcc ctgaccgcca atgccttgca gggcgaccgc gaaacctgcc tcgcggcagg
     6421 gatgaacgac tacctggcca agccgttcaa acgctccgac ctgagcctcg ccctgcagcg
     6481 ctggataggg tcgagaccat cgacgggtgg cgaatctgtg tgaaatacgg cacgcttgca
     6541 cattcgcaca gtcatggacg gcaccattcg tcgcagaatg accattgccg cgctgtgact
     6601 ttcatcaaca ggccatagtc tatgactagg tcgcaggcac gcttccatca gggagggcgt
     6661 ggccagaaag atctgccccc tgtggtacag ggaccgtcga ggagctcgca tgaccaaaca
     6721 aaacgccttc actcaggaag aactgctcag ctgcagccgc ggtgaactgt tcggcccggg
     6781 taacgcgcaa ctgcccgccc ccaatatgtt gatgatggac cgcatcgtcc acatcagcga
     6841 acacggcggc aagtacggca agggcgaaat cgtggccgag ctcgacatca acccggacct
     6901 gtggttcttc ggctgccact tcatcggtga cccggtgatg cccggctgcc tgggcctcga
     6961 cgccatgtgg cagctggtcg gcttctacct cggctggcag ggcaaccccg gccgcggccg
     7021 tgccctgggc tcgggcgaag taaaattctt cggccaggtc ttgccgaccg ccaagaaagt
     7081 cacctacaac atccatatca agcgcaccat cacccgctcg ctgatcctgg ccattgccga
     7141 cggcaccgtg agcgtagatg gccgcgagat ctacagtgcc gaaggcctgc gcgtcggcct
     7201 gttcacttcc actgatagct tttaagggtt acccgcatgc gtcgcgtcgt gatcaccggg
     7261 atgggcatcg tgtcctgcct gggcaatgac aaagagactg tttccgccaa cctgcgcgcc
     7321 agccgcccgg gcatccgctt caaccagtcc tatgccgaca tggggctgcg cagccaggtc
     7381 tccggctccg tcgacctcga cctcgaagcg ctgatcgacc gcaaggtcta ccgcttcatg
     7441 ggtgacgccg ccgccttcgc ctacctgtcc atggaacagg cgatcaagga tgccggcctg
     7501 accgaggaag agatctccaa cccgcgcgtc ggcctgatcg ccggctccgg tggcgcttcc
     7561 accctcaacc agatggaagc cctggacatc ctgcgcgaaa agggcgtcaa gcgcgtcggc
     7621 ccgtaccgcg tgccgcgcac catgggcagc accgtgtccg cctgcctggc caccccgttc
     7681 aagatcaagg gtgtgaacta caccatctcc tccgcttgcg ccaccagtgc ccactgcatc
     7741 ggcaacgcca tggagcagat ccagatgggc aagcaggaca tggtcttcgc cggcggcggt
     7801 gaagaggagc attggagcca gagcttcctg ttcgacgcca tgggcgcgct gtccacccag
     7861 tacaacgcca ctccggaaaa ggcctcgcgc gcctacgacg ccaagcgtga cggcttcgtc
     7921 atcgccggcg gcggcggcat ggtggtggtc gaggagctgg agcacgcgct caagcgcggc
     7981 gccaagatct acgccgagat cgtcggctac ggtgccactt ccgacggcta cgacatggtc
     8041 gccccgagcg gcgaaggcgc gatccgctgc atgcagcagg cgctggccac cgtcgacacc
     8101 ccgatcgact acctgaacac ccacggtacc tcgactccgg tcggcgacgt ggccgaagcc
     8161 cgtgccgtgc gcaccgtgtt cggcgacaag gctccggcca tcagctcgac caagagcctg
     8221 tccggccact cgctgggcgc cgccggggtg caggaagcga tctacagcct gctgatgatg
     8281 gaaggcaact tcatcgccgg ctcggccaac atcgacgagc tggacccgga agtggccgat
     8341 ctgccgatcg tcacccagac ccgcgagaac gtcaccctga acacggtgat gagcaacagc
     8401 ttcggcttcg gcggcaccaa cgccacgctg gtcctgaagc gctggaccgg caagtaagtc
     8461 gatctcgctg cactgcaacg gcaccttcgg gtgccgttgt cgtttctgcc tcccgcgctt
     8521 gtgcgtacag cccccgctgg cgcccacata ctccataggg tatacagaag atctgagccc
     8581 ggtggatgcc gcatgcggcc aggccgagcg catgggcagc cgccccaacc cgcaaaggag
     8641 agatgcagat gcaatgcaat gttggcaaga cggacaagat cctgcgcatc gtcgccggcc
     8701 tggtcctgtt ggcactcggt gccaccggcg tgatcggctg gtgggggttg atcggcttgg
     8761 tgccgctggc gaccggcctg ctgagctggt gcccggccta tggcctgctc ggcatcaaga
     8821 cctgcggcaa ggaccagtga gatgacgctg aagaccatcc gcgccagcgg caacctcgat
     8881 cacgccttcg ccatcagcgt ggaagccggc aaccatgtca tccgcatgga ccagccgaag
     8941 aacgccgccg gccaggacct cgggccgacg ccgctggaac tcatcctcgc cgccgtcgcc
     9001 ggctgcttcg gcaccatcgg ccgcttcctc gctcaccagc gcaaattcga gctgcgcggc
     9061 atgcagttcg agatcgaggg cgactacgac ccggacggcc tgctcggccg ccgcgacgac
     9121 gtgcgcccag gcttccaggc actgcgggtg aaggtggcaa tcgacgccga cctgactcgc
     9181 gaggagaagc aggcggtgct cgccgagatc gaacggcgct gcccgttggc cgacaacctg
     9241 gtcaacggca cccgtctgga aagcacgctg gcctgatccc ggccgcagaa acgacaaggc
     9301 ccgcagcaca gcgctgcggg ccttgtcgtt tctggcagcg atcagcgtac gtcggagcgc
     9361 tgctcagcca gcttgcggtc gccctggaac gccatgaagt gccactcgcc gggcacgact
     9421 tcgtagcgct cgctgaactc gcaggccatc acgtcctgca cggcagccgg cgcctgctgc
     9481 tgcaccccct cgaagcggtc gtgacgcttg ccgtccgggg tcaccacgct ggggtgaagt
     9541 acagcggggt atccttgacc ggcttgccgc tggggtgacc tgtccacggg caagcccgga
     9601 ccggcacaga cggtctatcc tgcactaacc gagcacgggc gcagcggggt gcggcatgga
     9661 caagaagttt caatggaacg cctggtattt cctgctggcc ttcagcctgc tgctgatgtt
     9721 ccagggctgg tggagcgagc gccaagccgt cgaacccatc gcctatagcg aattcctcaa
     9781 gctgctcaag gacggcaagc tgagcgagct gagcatcaat aaccagcaga tcagcggccg
     9841 gctgctggag ccgatcaaca accgcagcag cttcgtcacc aaccgcgtcg atcccgcgct
     9901 ggccgaggaa ctggccgcct ccggggccaa gttcaccgcg gtgcgcgaga acaccctggt
     9961 cgccaatctg ctgtcctggc tgctgccact ggtgctgatc ttcgccttct ggagcttcct
    10021 gttccgccgc ctgaccgaca agcagggcct cggcggcctg gtcagcgtcg gcaagtccaa
    10081 ggccaaggtg tttgtggagc gcgacaccgg agtgaccttc gaggacgtcg ccggcatcga
    10141 cgaagccaag gccgagctgg aagagatcgt ctccttcctc aaggaccagc ccaagtacag
    10201 ccgcctcggt gcgcgcattc ccaagggcat cctgctggtc ggcccgcccg gcaccggcaa
    10261 gaccctggtg gccaaggcgg tggccggcga agccggggtg ccgttcttct ccatctccgg
    10321 ctcggaattc gtcgagatgt tcgtcggcgt cggcgccgca cgggtgcgcg acctgttcga
    10381 gcaggcacgc aaggcggcgc cctgcatcat cttcatcgac gaactggacg ccctgggcaa
    10441 gatgcgcggg gtcggcgcct tcggcggcaa cgacgagaag gaacagaccc tcaaccagct
    10501 gctcgccgaa ctggacggct tcgacccccg cgaaggcgtg gtgctgctgg ccgccaccaa
    10561 ccgcccggaa atcctcgacc ccgcgctgct gcgcgccggc cgcttcgatc ggcagatcgt
    10621 cgtcgaccgc ccggaccgca agggtcgggt ggccatcctc aaggtgcacg tcaagcgcat
    10681 ccagatcgcc gccgacgtcg atctggacca ggtcgccggc atcacccccg gcttcaccgg
    10741 cgccgacctg gccaacctgg tcaacgaggc ggccatcctc gccacccgcc gcagcgccga
    10801 acaggtcgcc atggacgact tcacccgcgc ggtggagcgc atcgtcgccg gcgccgagcg
    10861 ccgcggccgc ctgctgctgc cccacgagcg cgaggtggtg gcctatcacg agatgggcca
    10921 cgcgctagtc gccagcagcc tgccgggcat ggacccggtg cacaaggtgt cgatcattcc
    10981 gcgctccatc ggcgccctcg gctacaccat gcagcggcca accgatgacc gcttcctgat
    11041 caccctcggc gagctgaaga accgcatggt ggtgctgctc gccggccgcg cggccgagca
    11101 gctgctgttc ggcgaaatct ccaccggcgc ggccgacgac ctggtcaagg tcaccgacat
    11161 cgcccggcag atcatcaccc gcttcggcat gagcgccgat ctcggccagg cggtgctgga
    11221 gcgccagagc aacagtttcc tcggcgacaa cctgattgcc atgcgcgaga aggactattc
    11281 cgagcagacc gcccgggaga tcgacctggc cgtgcgccag ttgatcgacg aggcctaccg
    11341 ccgcgccggc gaggtcctcc agcagcgccg cgccgaactg gatgagggcg cccgcctgct
    11401 gctcgagcgg gaaaccatca ctcccgaaga cttcgcgccg ctgcgcccgg aacgcgcggc
    11461 agcggcgccg tgagaggcac cgctagcggc atcccgcccg cggcggttgt cagcacacaa
    11521 ggacgtgcac atgatcggat ctcacccgca gcgcaaggca ggacagctcc cgtgtcgata
    11581 gaccatcgtc cgctgatcta ccaggcctac ctgtacaccc tggcctttgc cctgctggcg
    11641 gtcggcggga tcagcctgct cgacgtcctg ctgcaacaac agccgagccc gtaccgcatg
    11701 ctgttgctgc cagacagcgc cctgctggcg ctggccctgg gcgcgaccct gctggcggca
    11761 gtcctgcgcc agcgccgcgc ctgcctgctg gcctgcgcca gcgtacttct gctgatgagc
    11821 tacaccctgc tgtacaacaa cctgtacggg ggagccgagc aggggcgttc gctgctcggc
    11881 gacagcccgc gcctgcacag cgaaccggtg ttgctgctgg ggctgctggc ggtggtactc
    11941 ctcgccggct gccttgacgg gcgcctggca cgacggctgg gcgagctggc cggcgcgctg
    12001 gtcatggccc tgggactgct cggtctgctg gcccgcatat ggccggcgct ggcccccccc
    12061 gtaccgggct tcatgctcga ggttcccacc ctcagcggcc tgaccctgat cttcctcggc
    12121 ctggcggtgt tgctgctcaa ccggctgggc acgctgccgg gcctggcacc cttcgaccgc
    12181 cggaccctgc tggcaggcgt ctgtggcgtg ctgctgtcaa ccgccagctg ggctctgctc
    12241 agccagcaga gcatcaatgc cctctccgag cagagccagc tgctgctcgc gcgcttgcaa
    12301 agcgtcagcc gggagaccct gagtgcccgc ctggcgctga tccagcgcat ggccgagcgc
    12361 tggcaggcca gcgccagcat gccttccgcc cccctgtggc aacaggaggc cggcagctac
    12421 ctgcgcgact tccccaacct gggactgctc gccgtactgg acgaacgcct ggcgccccag
    12481 cgcagccagg tccgcgcgcc catggcccag ctctggctgg agagcttcct ggccgacccg
    12541 gcgcggcgcg gcgtgactgg ctgcagcagg tgcgcgccgg gcgccaggcg cagatgagcg
    12601 cctcgctgaa cctgctcggc aacggcccga tggcgctgat cgccgtcccc ctgcacctgc
    12661 cgggccagca agcacagctg ctggtggcca gcctggatgt cggcgacacc ctgggcagcc
    12721 tgctcggcgg caacctcggt gaattcatca tccgcgtgca cgagggtggc cagctgatct
    12781 acgactcggc cggtacccga ccggcgcacg atccgacccc ggtgggcagc ggccagaccc
    12841 gcctggcgca aaccccggag tggacgatcg gcacctatct cgaacccggg cgcggccagg
    12901 ccggcagtcg cttgctggcc atcctggtgc tgctcgccgg cctcgccctg agctttttcc
    12961 tgatgttcac ccagcgcctg gcctgggggg cccagcaaca ggcgctgcac ctgcgcgagc
    13021 ggcaacgctt cgacgaggcg cagagccgcg tgctggacat gatctccacc ctgcaaccgc
    13081 tgccgcagat cctcgaggca atctgccggc tgatcgaaca gcgcgatccg cgcctgcact
    13141 gctcgatcct gctggtcgac agcagcggcc agcgcctgac gctgggcgcc gcacccagcc
    13201 tgcccgagca ctacaaccag gccgtcgacg gcctgccgat ccgcgaaggg acgggctcct
    13261 gcggcaccgc cgccttccgc cgccaggcgg tgctggtcaa cgacattgcc cacgacccgc
    13321 tgtggcaggg cttccacgaa ctggccgcca gcagcgacct gcatgcctgc tggtcgaccc
    13381 cgctgttcgc cgccagcggc gatctgctcg gcaccttcgc catctaccag cagcaagcga
    13441 tggtggtgga taccgccctg gaggagctgg tcgccaccgc cagccatctg gccgccatcg
    13501 ccatcgagca caagaccacc cgccagcgcc tgcaggaaag cgagcaacgc ttccgctcgc
    13561 tgttcgcctg caatcccgac ccggtgttcg ccttcgacct gcgcggctgc ttcgagcggg
    13621 tcaatccggt cgccagccag ctgctcggct accgcgagga cgagctgcgc ggcgcccact
    13681 tcacccaata cctggcgccg accgaggcgc agcgcatcca gccgctgctg ctcaaggtgg
    13741 cacgcggcga aacccacgcc ttcgagctgc agtgccacaa ccgtgccggc gagctgctgg
    13801 agctggacgt cacccagctg ccgatcgtga tcgaccgcgc catcgtcggg gtgttctgca
    13861 tcgccaagga catccgccag cgcaagagcg ccgagcggac cctgcagagc accctggccg
    13921 agctggagcg cagcaaccga gaactgcagg agttcgcctt cgtcgcctcc cacgacctgc
    13981 aggagccgct gcgcaagatc cagaccttcg ccgagcggct gcggacccgc cccgccgggc
    14041 tggacgagca gggccgcgac tatctccagc gcatgagttc ggccgccgag cgcatgcagg
    14101 cgctgatcca cgacctgctg gcctactcgc gcgtcgccac ccgcacccaa ccgttcgtgc
    14161 gcctccacct ggagcgggtg ctcgacgccg tcctgcagga tctggagcat gtcgtcgagc
    14221 gcagcggcgc gcagatcgtc cgcgcgccgc tgcccgccat cgccggcgat ccgtcgcaaa
    14281 tgcgccagct gctgcagaac ctgctgagca acgcgatcaa gttccacaag ccgggcgagc
    14341 tgccacaggt gcgcatctat gctgaacatc aacccacggg cgagtggagc ctgtgcgtga
    14401 gtgaccaagg cgtggggttc gatgaaaaat acctcgaccg catcttccac ccgttccagc
    14461 gcctgcacag ccgccaggaa tacccgggca ccggcatcgg tctggcgatc gtcaacaaga
    14521 tcgccgaacg ccacggcgcc cgactgtcgg ccagcagcca gccggggcaa ggcgccacct
    14581 tccgcgtcac cttccccgcc gcagacaagg agtgaatggc catgtcgagc ccgctacccg
    14641 cgcccatcct gattgccgac gatgacccgg acgactgcct gctcgcccgc gaggccttca
    14701 gcgaaagccg cataccgaac cggctggagt tcgtccacga tggcgagcag ctgctcgact
    14761 acctgcacca ccggccgccc tacgacgacg cccggcgcta tcccctgccc ggcctgatcg
    14821 tcctcgacct gaacatgccg cgcatggatg gccgcaccgc gctgcgggcg ctgaaggacg
    14881 acccgcagct gcgacaaatt ccggtgatca tgctcagcac ctccagcgac gcagaagaca
    14941 tccgccgaag ctatgccgac ggggtaaact ccttcatcag caagccggcc agctacagcg
    15001 gcctgctcga agtagtccgc agtctcagcg gctactggct gaacaccgtc gaactgccgc
    15061 tggagccgcc accctcatga ccctgcccga cgcccccctg cgcctgctgc tgatcgagga
    15121 tgacgaggac gacttcctga tcatccgcga cctgctgggc gaagccggca acctgcatta
    15181 cgtgctggac tgggcagaca gcttcgagcg cggccgctgc gccatcgaca gtggcgtgca
    15241 cgacctgtat ctgatcgaca accgtctcgg caccgactcc ggcctggagc tggtgcgcca
    15301 tgccatcgct cgcaacatgc atcagccact gatcctgctc accggccggg acgacagcga
    15361 actcgacgcc cgcgccctcg aactgggcgc ctcggactac ctggtcaagg gccagtttga
    15421 cgggcgtctg ctcgggcgca gcatccgcta tgcgctggag cgcgccagtg cgcgcgagcg
    15481 cctggggcgc agcgacaacc gagccgccct gctgcagcgc tgcctggaag ccagctacaa
    15541 cggcgtgctg atcgtcgatg cgcaggccgc ggaccagccg atcatctatg ccaacccggc
    15601 cttcgagcgc atcaccggct accgcgagga agaggtactg gggcgcaact gccgctttct
    15661 gcagggcggc gaaacccgcc agcccgccat cggcgagatc cgccgcagcc tggccgagca
    15721 ccgcgaggtg cacgtggtgc tgcgcaactt ccgcaaggat ggcagcccct tctggaacga
    15781 cctgtacatc gccccggtgc cggacgagcg cggcgtggtc acccactacg tcggcgtgca
    15841 gaacgacatt tccgagcgca agcgcttcga gtcggagctg gcctacaacg ccagccacga
    15901 cgtgctgacc ggcctgccca accgctcgct gctggaggac cgcctgctgc agggctgcca
    15961 gatcgcccgg cgctactcgc gggagctggc ggtgatgctg atcgacctcg atggcttcaa
    16021 gccgatcaac gacaccctcg gccacgcggt cggcgacaag gtgctggtcg aggtggcgca
    16081 gcgcatgggc gcacagatcc gccccggcga caccctcgcc cgcctcggcg gcgacgagtt
    16141 cgtcgtcctg ctgccggacc tggcccatgg cgaggacgtg ctgctggtgg ccgagcgcct
    16201 gatccgcagc atctcgcgcc cctaccgcct caacgaactg gaactgcacg tcaccgccag
    16261 tatcggcatc accctcagcg acggcgagat cgagcagccg atgaagctga tccagcaggc
    16321 cgacctggcc atgtaccaag ccaagcagca cggccgcaac aactaccagt ggtacaccga
    16381 gcacctcaac caggaagtca gcgagcgcat gaccctgcgc aacgagctgc agaaagccat
    16441 cgagagcatg agcttcgagc tgcactacca gccgcagatc gacgcccaca gcggcgcggt
    16501 gatcggcagc gaggccctgc tgcgctggcc gcatgccgag cgcggcttga tctcgccgct
    16561 gcgcttcatc ccggtcgccg aggacaccgg gcagattatt cccatcagcc agtgggtgct
    16621 ggacaccgcc tgccggcaca accgccagtt gctcgaccgc ggcctggccc gcggcgccat
    16681 ctcggtgaac atctcggcgg tgcacttcct gcgcagcaac ttcatcgaga ccgtgcgccg
    16741 cgcactggac agcagcggcc tgccggcgag cctgctggag ctggaaatca ccgaaagcgt
    16801 gctgctgaag aacgccgagc gggcgatcta tctgctccac gaactgaaga aaatcggcgt
    16861 cggcctggca ctggacgact tcggcaccgg ctattccagc ctcagctacc tcaaggatct
    16921 gccgatcgac aaggtcaaga tcgaccgctc cttcgttcag gagctgatca gcgacccgcg
    16981 cgacgccgcc atcacccagg gcatcatctc catggcccat cacctcaacc tcaaggtggt
    17041 ggccgagggc gtggaaagcg ccgagcaggc cgccttcctc aaccgcaacc agtgcgacgc
    17101 cttccagggc ttcctctacg ccaggccgat gccgtttgcc gagctggagg gctatctgca
    17161 gcggcaggcg cagctggaga tggccgggca ctgagcgtgc gctgcgggca cggcttgctc
    17221 cccgccagcg gacttgctgc cagccctcag tagtcgcgct tgtagaagat atccagcgaa
    17281 ctggccaggc cgctggccgc ttccaggtag agcttcttgc ccagttcgta gcgcagggca
    17341 aagatgttgc cctgctcgaa cacgccgacg ccgtagcgca ggctgagctt ctccgagagg
    17401 taaccactgg cgaacacgct gctgctggtg ccggagcctt cactgtccag ctgaaaatcc
    17461 ctgaccccga ggcgctgggc gatcgccccg gctaccggcg tgccgccggc taggcccagg
    17521 gccaaggcgg cctggccgac caggttgctg tcgccgctgc tgcccggcgc gcgacccagc
    17581 accagatagg acagcgcctg ctcctggctc atcgccggtt cggagaacac ctcgctgacc
    17641 ggggcgtcgg cgcgcccggt caggcgcagg ccggcagtca cttcatcgac ggtgcggatc
    17701 gcctcgatgt ccagcagcgg ctggtcgatc ggcccggcga agatcagctg ggcgcggcgc
    17761 agggtcaggc gctggccgta ggcgcggtag cggccgtttt ccagcttcag ggtgccgcgg
    17821 ctgtcgaggt tgtccttgat gcgcagatgg cccttgaggt cggcgctcag gccgaagccg
    17881 gagaagcgca ggcggtcctc gccgagcgcc agacgcaggt ccatcttcag ccccggcatg
    17941 cgcgccgcgg ccgtctgctc gccgatgatc tgcgcatccc ccgagaccgt gaccgcctgg
    18001 gccggcaact cgcgcacctt gatctgcccg cgcggaatgg cgagctggcc gccgaggctg
    18061 aactggtggt cgcgcaggcc gagctgcagg tccggctcga cctcgacgct ggcatagggc
    18121 tcgaccaccg ccggcaggcg gctgccgcga atctgcaggt ccatctgcgg cgcgaccgcc
    18181 cagtccaggc tgccggtcag cgcgccctgc ccgtgctcgc cgctgcgcca gcggccatcg
    18241 accagcgcct gctggccagc gatggtcatg gtcagttgca gcgcctcgaa ggtcagcggc
    18301 agctcgccgc cgctgacatg gccatcgcgc aattgcacgc ggccgttgac taccggcgcc
    18361 agcaggctgc cggacagctg gccgcggccg tcgatgcgcc cctcgagacg ctcgacctgg
    18421 ctgacgaagg gtcgcgccag ggcgagatcc aggccgtcga ggcggaagct gccggacagc
    18481 ggccggctgg ccgggcgcgg gtcgatgacg gtgtccagct ccagggtgcc caagctcggt
    18541 ccggccaggc gcagctggct atcgacccgc tggggccgca gctggctgac caggctgagc
    18601 tgccggtagg ggacatccag ccactgctca tggccgtcgg tccgctggcg caggcgcagc
    18661 tggccgctgc cggcatccag gcggagttcg ccgctcggcc cgctggccgg caggtcgaga
    18721 cgcagatcgc cgccgagctg gccgaccatg gcgaaatcct ccggcagggc gccggccagc
    18781 cgcgccaggt cgaacccccg caggcgcatc cgcagctgtg gatcgggcag cagacgctgg
    18841 gcgtcggcgc acagcctggc ctgcgccgac tgccagcagt gcgcgccgag ggtc
//
